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Science (New York, N.Y.)
03 26, 2021;371 (6536): 1350-1355.

Molecular mechanism of cytokinin-activated cell division in .

Weibing Yang, Sandra Cortijo, Niklas Korsbo, Pawel Roszak, Katharina Schiessl, Aram Gurzadyan, Raymond Wightman, Henrik Jönsson, Elliot Meyerowitz
Author Information
  1. Weibing Yang: Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK. ORCID
  2. Sandra Cortijo: Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK. ORCID
  3. Niklas Korsbo: Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK. ORCID
  4. Pawel Roszak: Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK. ORCID
  5. Katharina Schiessl: Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK. ORCID
  6. Aram Gurzadyan: Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK. ORCID
  7. Raymond Wightman: Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK. ORCID
  8. Henrik Jönsson: Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK. meyerow@caltech.edu henrik.jonsson@slcu.cam.ac.uk. ORCID
  9. Elliot Meyerowitz: Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK. meyerow@caltech.edu henrik.jonsson@slcu.cam.ac.uk. ORCID
PMID: 33632892 DOI: 10.1126/science.abe2305

Abstract

Mitogens trigger cell division in animals. In plants, cytokinins, a group of phytohormones derived from adenine, stimulate cell proliferation. Cytokinin signaling is initiated by membrane-associated histidine kinase receptors and transduced through a phosphorelay system. We show that in the shoot apical meristem (SAM), cytokinin regulates cell division by promoting nuclear shuttling of Myb-domain protein 3R4 (MYB3R4), a transcription factor that activates mitotic gene expression. Newly synthesized MYB3R4 protein resides predominantly in the cytoplasm. At the G2-to-M transition, rapid nuclear accumulation of MYB3R4-consistent with an associated transient peak in cytokinin concentration-feeds a positive feedback loop involving importins and initiates a transcriptional cascade that drives mitosis and cytokinesis. An engineered nuclear-restricted MYB3R4 mimics the cytokinin effects of enhanced cell proliferation and meristem growth.

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Grants

  1. /Howard Hughes Medical Institute

MeSH Term

Active Transport, Cell Nucleus
Arabidopsis
Arabidopsis Proteins
Cell Cycle Checkpoints
Cell Division
Cell Nucleus
Cytokinins
Cytoplasm
Gene Expression Regulation, Plant
Karyopherins
Meristem
Mitosis
Plant Growth Regulators
Signal Transduction
Trans-Activators

Chemicals

Arabidopsis Proteins
Cytokinins
Karyopherins
MYB3R1 protein, Arabidopsis
MYB3R4 protein, Arabidopsis
Plant Growth Regulators
Trans-Activators
Journal Article Research Support, Non-U.S. Gov't

Links to CNCB-NGDC Resources

GEN: GEND000322 (Transcriptional regulation of cell division and cytokinin response in Arabidopsis shoot apical meristem)

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