extracts: Phytochemical screening, antibacterial and antioxidant activities.

Rafael Viteri, Ady Giordano, Gloria Montenegro, Flavia Zacconi
Author Information
  1. Rafael Viteri: Organic Chemistry Department, Faculty of Chemistry and of Pharmacy, Pontificia Universidad Católica de Chile, Santiago, Chile.
  2. Ady Giordano: Inorganic Chemistry Department, Faculty of Chemistry and of Pharmacy, Pontificia Universidad Católica de Chile, Santiago, Chile.
  3. Gloria Montenegro: Plant Science Department, Faculty of Agriculture and Forestry, Pontificia Universidad Católica de Chile, Santiago, Chile.
  4. Flavia Zacconi: Organic Chemistry Department, Faculty of Chemistry and of Pharmacy, Pontificia Universidad Católica de Chile, Santiago, Chile. ORCID

Abstract

is a native and dominant species from the Temperate Forest in the southern region of Chile. It is a crucial melliferous species producing unifloral honey with outstanding antibacterial properties with great commercial value at international markets. A phytochemical screening was developed by colorimetric and gravimetric methods. Antioxidant activities were assessed by using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and ferric ion reducing antioxidant power (FRAP) method. The minimum bactericidal concentration (MBC) was the method used to determine the antibacterial activity. The results revealed the highest antioxidant activity for the methanolic extracts of branches (IC 35.7 µg/mL for DPPH, IC 11.0 µg/mL for ABTS, and 4.6 mM FeSO·7HO/g in FRAP), attributed to its high content of total phenols (332.8 mg GAE/g). The quantification of the MBC led to values in the range of 0.78-12.5 mg/mL for and , and 6.25-50 mg/mL for and , respectively. The presented work provides valuable first evidence regarding the potential application of extracts as antioxidants and antibacterial agents in the nutraceutical and phytopharmaceutical industry.

Keywords

MeSH Term

Anti-Bacterial Agents
Antioxidants
Escherichia coli
Microbial Sensitivity Tests
Phytochemicals
Plant Extracts
Staphylococcus aureus

Chemicals

Anti-Bacterial Agents
Antioxidants
Phytochemicals
Plant Extracts

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