OpenLB
Open Library of Bioscience
Advanced Search
Advanced healthcare materials
04, 2022;11 (7): e2101426.

Biopolymer Nano-Network for Antimicrobial Peptide Protection and Local Delivery.

Natthaporn Klubthawee, Giovanni Bovone, Bruno Marco-Dufort, Elia A Guzzi, Ratchaneewan Aunpad, Mark W Tibbitt
Author Information
  1. Natthaporn Klubthawee: Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathum Thani, 12120, Thailand.
  2. Giovanni Bovone: Macromolecular Engineering Laboratory, Department of Mechanical and Process Engineering, ETH Zurich, Zurich, 8092, Switzerland. ORCID
  3. Bruno Marco-Dufort: Macromolecular Engineering Laboratory, Department of Mechanical and Process Engineering, ETH Zurich, Zurich, 8092, Switzerland. ORCID
  4. Elia A Guzzi: Macromolecular Engineering Laboratory, Department of Mechanical and Process Engineering, ETH Zurich, Zurich, 8092, Switzerland. ORCID
  5. Ratchaneewan Aunpad: Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathum Thani, 12120, Thailand.
  6. Mark W Tibbitt: Macromolecular Engineering Laboratory, Department of Mechanical and Process Engineering, ETH Zurich, Zurich, 8092, Switzerland. ORCID
PMID: 34936732 DOI: 10.1002/adhm.202101426

Abstract

Antimicrobial resistance (AMR) develops when bacteria no longer respond to conventional antimicrobial treatment. The limited treatment options for resistant infections result in a significantly increased medical burden. Antimicrobial peptides offer advantages for treatment of resistant infections, including broad-spectrum activity and lower risk of resistance development. However, sensitivity to proteolytic cleavage often limits their clinical application. Here, a moldable and biodegradable colloidal nano-network is presented that protects bioactive peptides from enzymatic degradation and delivers them locally. An antimicrobial peptide, PA-13, is encapsulated electrostatically into positively and negatively charged nanoparticles made of chitosan and dextran sulfate without requiring chemical modification. Mixing and concentration of oppositely charged particles form a nano-network with the rheological properties of a cream or injectable hydrogel. After exposure to proteolytic enzymes, the formed nano-network loaded with PA-13 eliminates Pseudomonas aeruginosa during in vitro culture and in an ex vivo porcine skin model while the unencapsulated PA-13 shows no antibacterial effect. This demonstrates the ability of the nano-network to protect the antimicrobial peptide in an enzyme-challenged environment, such as a wound bed. Overall, the nano-network presents a useful platform for antimicrobial peptide protection and delivery without impacting peptide bioactivity.

Keywords

antimicrobial peptides antimicrobial resistance biopolymer nanoparticles nano-networks peptide stability

References

ACS Biomater Sci Eng. 2021 Sep 13;7(9):4102-4127 [PMID: 34137581]
Pharmaceutics. 2018 Jan 18;10(1): [PMID: 29346275]
RSC Adv. 2018 Feb 16;8(14):7533-7549 [PMID: 35539132]
Adv Wound Care (New Rochelle). 2013 Oct;2(8):438-447 [PMID: 24688830]
Front Cell Infect Microbiol. 2018 Jun 15;8:196 [PMID: 29963501]
Wound Repair Regen. 2021 Mar;29(2):316-326 [PMID: 33480137]
Sci Rep. 2020 Jun 4;10(1):9117 [PMID: 32499514]
ACS Nano. 2013 May 28;7(5):4194-201 [PMID: 23638642]
Pharmaceuticals (Basel). 2013 Aug 21;6(8):1055-81 [PMID: 24276381]
Microorganisms. 2017 Mar 07;5(1): [PMID: 28272369]
Int J Mol Sci. 2021 Jan 11;22(2): [PMID: 33440780]
Front Microbiol. 2021 Jan 13;11:592220 [PMID: 33519729]
Int J Antimicrob Agents. 2009 Nov;34(5):457-61 [PMID: 19524411]
J Pharm Sci. 1999 Sep;88(9):933-7 [PMID: 10479357]
Pharmaceutics. 2020 Sep 02;12(9): [PMID: 32887353]
Adv Healthc Mater. 2022 Apr;11(7):e2101426 [PMID: 34936732]
Antimicrob Agents Chemother. 2009 Feb;53(2):593-602 [PMID: 19029324]
Acta Biochim Biophys Sin (Shanghai). 2017 Oct 1;49(10):916-925 [PMID: 28981608]
J R Army Med Corps. 1993 Jun;139(2):49-51 [PMID: 8355236]
Lancet Infect Dis. 2005 Sep;5(9):568-80 [PMID: 16122680]
RSC Adv. 2020 Oct 22;10(64):38746-38754 [PMID: 35518403]
PLoS One. 2010 Sep 10;5(9): [PMID: 20844765]
Biomacromolecules. 2004 Sep-Oct;5(5):1882-92 [PMID: 15360302]
Appl Microbiol Biotechnol. 2013 Apr;97(7):3085-96 [PMID: 23053090]
Front Neurosci. 2017 Feb 14;11:73 [PMID: 28261050]
Lancet Infect Dis. 2005 Aug;5(8):481-93 [PMID: 16048717]
Biomaterials. 2010 Jun;31(18):4980-6 [PMID: 20303585]
Int J Pharm. 2003 Nov 6;266(1-2):29-37 [PMID: 14559391]
J Infect Public Health. 2013 Dec;6(6):410-5 [PMID: 23999348]
J Clin Microbiol. 2000 Feb;38(2):918-22 [PMID: 10655417]
AAPS PharmSciTech. 2007 Nov 30;8(4):E98 [PMID: 18181558]
Part Fibre Toxicol. 2016 Feb 29;13:12 [PMID: 26926244]
Am J Respir Crit Care Med. 2002 Apr 1;165(7):867-903 [PMID: 11934711]
Methods Mol Biol. 2010;618:359-69 [PMID: 20094875]
Trends Mol Med. 2014 Mar;20(3):166-78 [PMID: 24380647]
ACS Appl Mater Interfaces. 2019 Feb 20;11(7):6741-6750 [PMID: 30676016]
Eur J Pharm Biopharm. 2004 Mar;57(2):163-9 [PMID: 15018971]
Lancet. 2011 Jan 15;377(9761):228-41 [PMID: 21146207]
Trop Med Infect Dis. 2021 May 18;6(2): [PMID: 34070228]
Environ Technol. 2007 Dec;28(12):1357-63 [PMID: 18341146]
Nat Nanotechnol. 2018 Jan;13(1):65-71 [PMID: 29203912]
J Biomed Nanotechnol. 2014 May;10(5):864-70 [PMID: 24734539]
Eur J Pharm Biopharm. 2011 May;78(1):10-8 [PMID: 21138767]
Molecules. 2019 May 21;24(10): [PMID: 31117310]
Arch Dermatol. 1998 Aug;134(8):1006-9 [PMID: 9722732]
Vet Pathol. 2012 Mar;49(2):344-56 [PMID: 21441112]
Drug Discov Today. 2006 Oct;11(19-20):905-10 [PMID: 16997140]
ACS Appl Mater Interfaces. 2014 Jul 9;6(13):10005-13 [PMID: 24938653]
Skin Res Technol. 2007 Feb;13(1):19-24 [PMID: 17250528]
Adv Mater. 2017 Mar;29(11): [PMID: 28067959]
PLoS One. 2012;7(8):e39373 [PMID: 22879874]
Nanoscale. 2017 Oct 19;9(40):15379-15389 [PMID: 28975171]
Lancet Infect Dis. 2020 Sep;20(9):e216-e230 [PMID: 32653070]
Adv Mater. 2011 Mar 25;23(12):H119-24 [PMID: 21394793]
Am J Clin Dermatol. 2011 Jun 1;12(3):157-69 [PMID: 21469761]
J Am Chem Soc. 2007 Dec 19;129(50):15615-22 [PMID: 18041836]
Adv Drug Deliv Rev. 2007 Sep 30;59(11):1152-61 [PMID: 17889400]
J Pept Sci. 2017 Nov;23(11):824-832 [PMID: 28833783]
Biomaterials. 1999 Sep;20(18):1701-8 [PMID: 10503971]
Bioconjug Chem. 2019 Feb 20;30(2):305-324 [PMID: 30428665]
Curr Cardiol Rep. 2013 Apr;15(4):346 [PMID: 23420442]

MeSH Term

Animals
Anti-Bacterial Agents
Anti-Infective Agents
Antimicrobial Peptides
Chitosan
Microbial Sensitivity Tests
Peptides
Pseudomonas aeruginosa
Swine

Chemicals

Anti-Bacterial Agents
Anti-Infective Agents
Antimicrobial Peptides
Peptides
Chitosan
Journal Article Research Support, Non-U.S. Gov't

Word Cloud

Similar Articles

Cited By