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International journal of molecular sciences
Aug 03, 2022;23 (15):

Identification of Diverse Stress-Responsive Xylem Sap Peptides in Soybean.

Wai-Ching Sin, Hon-Ming Lam, Sai-Ming Ngai
Author Information
  1. Wai-Ching Sin: Center for Soybean Research of the State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, China. ORCID
  2. Hon-Ming Lam: Center for Soybean Research of the State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, China. ORCID
  3. Sai-Ming Ngai: Center for Soybean Research of the State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, China.
PMID: 35955768 DOI: 10.3390/ijms23158641

Abstract

Increasing evidence has revealed that plant secretory peptides are involved in the long-distance signaling pathways that help to regulate plant development and signal stress responses. In this study, we purified small peptides from soybean () xylem sap via hlorophenol extraction and conducted an in-depth peptidomic analysis using a mass spectrometry (MS) and bioinformatics approach. We successfully identified 14 post-translationally modified peptide groups belonging to the peptide families CEP (C-terminally encoded peptides), CLE (CLAVATA3/embryo surrounding region-related), PSY (plant peptides containing tyrosine sulfation), and XAP (xylem sap-associated peptides). Quantitative PCR (qPCR) analysis showed unique tissue expression patterns among the peptide-encoding genes. Further qPCR analysis of some of the peptide-encoding genes showed differential stress-response profiles toward various abiotic stress factors. Targeted MS-based quantification of the nitrogen deficiency-responsive peptides, GmXAP6a and GmCEP-XSP1, demonstrated upregulation of peptide translocation in xylem sap under nitrogen-deficiency stress. Quantitative proteomic analysis of overexpression in hairy soybean roots revealed that significantly impacts stress response-related proteins. This study provides new insights that root-to-shoot peptide signaling plays important roles in regulating plant stress-response mechanisms.

Keywords

C-terminally encoded peptide CLV3/ESR-related long-distance peptide signaling peptidomic post-translational modification root-to-shoot sulfated peptide xylem sap

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Grants

  1. AoE/M-403/16/Innovation Technology Fund of Innovation Technology Commission: Funding Support to State Key Laboratory of Agrobiotechnology and RGC-Area of Excellence Scheme

MeSH Term

Humans
Nitrogen
Peptides
Plant Proteins
Plant Roots
Proteomics
Glycine max
Xylem

Chemicals

Peptides
Plant Proteins
Nitrogen
Journal Article

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