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Methods in molecular biology (Clifton, N.J.)
2023;2571 149-155.

Metabolomics Analysis of Aqueous Humor Based on Liquid Chromatography-Mass Spectrometry.

Karolina Pietrowska, Diana Anna Dmuchowska, Adam Kretowski, Michal Ciborowski
Author Information
  1. Karolina Pietrowska: Metabolomics Laboratory, Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland.
  2. Diana Anna Dmuchowska: Department of Ophthalmology, Medical University of Bialystok, Bialystok, Poland.
  3. Adam Kretowski: Metabolomics Laboratory, Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland.
  4. Michal Ciborowski: Metabolomics Laboratory, Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland. michal.ciborowski@umb.edu.pl.
PMID: 36152159 DOI: 10.1007/978-1-0716-2699-3_15

Abstract

Aqueous humor (AH) is a transparent fluid that fills the anterior segment of the eye. The composition and level of metabolites in AH are important for understanding its physiology and changes caused by the occurrence of eye disease. A simple method for the preparation and analysis of AH samples was developed using the liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) technique. The analyses were performed using two types of chromatography: reversed-phase liquid chromatography-mass spectrometry (LC-RP-MS) and hydrophilic interaction liquid chromatography-mass spectrometry (LC-HILIC-MS), in the sample prepared by one protocol.

Keywords

Aqueous humor HILIC analysis LC–MS Reversed-phase analysis Untargeted metabolomics

References

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MeSH Term

Aqueous Humor
Chromatography, Liquid
Chromatography, Reverse-Phase
Hydrophobic and Hydrophilic Interactions
Mass Spectrometry
Metabolomics
Journal Article

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