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Marine biotechnology (New York, N.Y.)
Feb, 2023;25 (1): 192-203.

Cost-Efficient Production of the Sphingan WL Gum by Sphingomonas sp. WG Using Molasses and Sucrose as the Carbon Sources.

Jianlin Liu, Hui Li, Xuanyu Zhang, Lin Yue, Wei Lu, Shaohua Ma, Ziyu Zhu, Dong Wang, Hu Zhu, Jiqian Wang
Author Information
  1. Jianlin Liu: State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering and Biotechnology, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, Shandong, People's Republic of China.
  2. Hui Li: State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering and Biotechnology, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, Shandong, People's Republic of China.
  3. Xuanyu Zhang: State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering and Biotechnology, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, Shandong, People's Republic of China.
  4. Lin Yue: State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering and Biotechnology, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, Shandong, People's Republic of China.
  5. Wei Lu: State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering and Biotechnology, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, Shandong, People's Republic of China.
  6. Shaohua Ma: Petroleum Industry Training Center, China University of Petroleum (East China), Qingdao, Shandong, People's Republic of China.
  7. Ziyu Zhu: School of Resources and Environment, University of Jinan, Jinan, 250022, People's Republic of China.
  8. Dong Wang: State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering and Biotechnology, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, Shandong, People's Republic of China.
  9. Hu Zhu: College of Chemistry and Materials Science, Fujian-Taiwan Science and Technology Cooperation Base of Biomedical Materials and Tissue Engineering, Engineering Research Center of Industrial Biocatalysis, Fujian Normal University, Fuzhou, Fujian, People's Republic of China. zhuhu@fjnu.edu.cn.
  10. Jiqian Wang: State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering and Biotechnology, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, Shandong, People's Republic of China. jqwang@upc.edu.cn.
PMID: 36635576 DOI: 10.1007/s10126-022-10193-1

Abstract

The polysaccharide WL gum is produced by the marine microorganism Sphingomonas sp. WG and presents great commercial utility potential in many industries especially in oil industries. However, the high fermentation cost limits its wide application. Therefore, an efficient production system at a lower cost was established using beet molasses to partially replace the commonly used carbon sources. Four different molasses were screened and their composition was investigated. One-factor design and RSM statistical analysis were employed to optimize the WL gum fermentation medium. The effects of molasses on the rheological properties and gene expression of WL gum were also investigated. The results showed that the pretreated beet molasses generated both high broth viscosity and WL gum production (12.94 Pa·s and 11.16 g/L). Heavy metal ions and ash were found to be the key factors in unpretreated and pretreated molasses affecting WL production. The cost-efficient production medium contained (g/L): sucrose 61.79, molasses 9.95, yeast extract 1.23, KHPO 1, MgSO 0.1, ZnSO 0.1 and the WL gum production reached 40.25 ± 1.15 g/L. The WL gum product WL-molasses showed the higher apparent viscosity, and viscous modulus and elastic modulus than WL-sucrose and WL-mix, which might be related to its highest molecular mass. The higher expressional level of genes such as pgm, ugp, ugd, rmlA, welS, and welG in WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity. This work provided a cost-efficient method for WL gum production.

Keywords

Cost-efficient production system Gene expressional level Molasses composition Rheological properties Sphingomonas sp. WG WL gum

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Grants

  1. 31800075/National Natural Science Foundation of China
  2. U1805234/National Natural Science Foundation of China
  3. 20CX02202A/Fundamental Research Funds for the Central Universities and the Development Fund of State Key Laboratory of Heavy Oil Processing
  4. ERCIB2020-01/Engineering Research Center of Industrial Biocatalysis, Fujian Province Universities
  5. 2015AA020925/National High-tech Research and Development Program
  6. 2021D039/Fujian-Taiwan Science and Technology Cooperation Base of Biomedical Materials and Tissue Engineering

MeSH Term

Sphingomonas
Sucrose
Molasses
Carbon
Fermentation
Culture Media

Chemicals

sphingan
Sucrose
Carbon
Culture Media
Journal Article

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