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02 09, 2023;13 (2):

Spatio-Temporal Expression Pattern of CAKUT Candidate Genes and during Human Kidney Development.

Daniela Veljačić Visković, Mirela Lozić, Martina Vukoja, Violeta Šoljić, Katarina Vukojević, Merica Glavina Durdov, Natalija Filipović, Bernarda Lozić
Author Information
  1. Daniela Veljačić Visković: Paediatric Diseases Department, University Hospital of Split, Spinčićeva 1, 21 000 Split, Croatia. ORCID
  2. Mirela Lozić: Department of Anatomy, Histology and Embryology, University of Split School of Medicine, Šoltanska 2, 21 000 Split, Croatia. ORCID
  3. Martina Vukoja: Laboratory of Morphology, Department of Histology and Embryology, School of Medicine, University of Mostar, 88 000 Mostar, Bosnia and Herzegovina.
  4. Violeta Šoljić: Laboratory of Morphology, Department of Histology and Embryology, School of Medicine, University of Mostar, 88 000 Mostar, Bosnia and Herzegovina.
  5. Katarina Vukojević: Department of Anatomy, Histology and Embryology, University of Split School of Medicine, Šoltanska 2, 21 000 Split, Croatia. ORCID
  6. Merica Glavina Durdov: Department of Pathology, University Hospital Split, 21 000 Split, Croatia. ORCID
  7. Natalija Filipović: Department of Anatomy, Histology and Embryology, University of Split School of Medicine, Šoltanska 2, 21 000 Split, Croatia. ORCID
  8. Bernarda Lozić: Paediatric Diseases Department, University Hospital of Split, Spinčićeva 1, 21 000 Split, Croatia.
PMID: 36830709 DOI: 10.3390/biom13020340

Abstract

We aimed to investigate expression of the novel susceptibility genes for CAKUT, and , proposed by a systematic in silico approach, in developing and postnatal healthy human kidneys to provide information about their spatiotemporal expression pattern. We analyzed expression of their protein products by immunohistochemistry and immunofluorescence and quantified relative mRNA levels by RT-qPCR. Statistically significant differences in expression patterns were observed between certain developmental stages. Strong expression of DLG1 was observed in the developing kidney, with a gradual decrease from the first phase of kidney development (Ph1) until the third phase (Ph3), when most nephrons are formed; at later stages, the highest expression was observed in the tubules. KIF12 was highly expressed in the developing structures, especially in Ph1, with a gradual decrease until the postnatal phase, which would indicate a significant role in nephrogenesis. Co-localization of DLG1 and KIF12 was pronounced in Ph1, especially on the apical side of the tubular epithelial cells. Thereafter, their expression gradually became weaker and was only visible as punctate staining in Ph4. The direct association of with as control genes of normal kidney development may reveal their new functional aspect in renal tubular epithelial cells.

Keywords

DLG1 KIF12 Ki-67 kidney development α-tubulin

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Grants

  1. IP-06-2016-2575/Croatian Science Foundation
  2. CRP/BIH18-03/International Centre for Genetic Engineering and Biotechnology

MeSH Term

Humans
Kidney
Vesico-Ureteral Reflux
Nephrons
Urogenital Abnormalities
Discs Large Homolog 1 Protein
Kinesins

Chemicals

DLG1 protein, human
Discs Large Homolog 1 Protein
KIF12 protein, human
Kinesins
Journal Article Research Support, Non-U.S. Gov't

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