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International journal of molecular sciences
Feb 14, 2023;24 (4):

Effect of Nanosecond Pulsed Currents on Directions of Cell Elongation and Migration through Time-Lapse Analysis.

Hayato Tada, Satoshi Uehara, Chia-Hsing Chang, Ken-Ichi Yano, Takehiko Sato
Author Information
  1. Hayato Tada: Institute of Fluid Science, Tohoku University, Sendai 980-8577, Japan.
  2. Satoshi Uehara: Institute of Fluid Science, Tohoku University, Sendai 980-8577, Japan.
  3. Chia-Hsing Chang: Institute of Fluid Science, Tohoku University, Sendai 980-8577, Japan.
  4. Ken-Ichi Yano: Institute of Industrial Nanomaterials, Kumamoto University, Kumamoto 860-0862, Japan.
  5. Takehiko Sato: Institute of Fluid Science, Tohoku University, Sendai 980-8577, Japan.
PMID: 36835235 DOI: 10.3390/ijms24043826

Abstract

It is generally known that cells elongate perpendicularly to an electric field and move in the direction of the field when an electric field is applied. We have shown that irradiation of plasma-simulated nanosecond pulsed currents elongates cells, but the direction of cell elongation and migration has not been elucidated. In this study, a new time-lapse observation device that can apply nanosecond pulsed currents to cells was constructed, and software to analyze cell migration was created to develop a device that can sequentially observe cell behavior. The results showed nanosecond pulsed currents elongate cells but do not affect the direction of elongation and migration. It was also found the behavior of cells changes depending on the conditions of the current application.

Keywords

agarose bridge cell behavior cell morphology chemical species free discharge-simulated electric current electric field microchannel migration velocity plasma medicine

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Grants

  1. 16H02311/Japan Society for the Promotion of Science
  2. 19H00743/Japan Society for the Promotion of Science
  3. 19H04271/Japan Society for the Promotion of Science

MeSH Term

Time-Lapse Imaging
Electricity
Cell Movement
Journal Article

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