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07 26, 2023;13 (1): 12133.

Sericin coated thin polymeric films reduce keratinocyte proliferation via the mTOR pathway and epidermal inflammation through IL17 signaling in psoriasis rat model.

Pornanong Aramwit, Kamonpan Fongsodsri, Khwanchanok Tuentam, Onrapak Reamtong, Tipparat Thiangtrongjit, Tapanee Kanjanapruthipong, Vamsi K Yadavalli, Sumate Ampawong
Author Information
  1. Pornanong Aramwit: Bioactive Resources for Innovative Clinical Applications Research Unit, Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok, 10330, Thailand.
  2. Kamonpan Fongsodsri: Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Ratchathewi, Bangkok, 10400, Thailand.
  3. Khwanchanok Tuentam: Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Ratchathewi, Bangkok, 10400, Thailand.
  4. Onrapak Reamtong: Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Ratchawithi Road, Ratchathewi, Bangkok, 10400, Thailand.
  5. Tipparat Thiangtrongjit: Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Ratchawithi Road, Ratchathewi, Bangkok, 10400, Thailand.
  6. Tapanee Kanjanapruthipong: Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Ratchathewi, Bangkok, 10400, Thailand.
  7. Vamsi K Yadavalli: Department of Chemical and Life Science Engineering, Virginia Commonwealth University, 601 W Main Street, Richmond, VA, 23284, USA.
  8. Sumate Ampawong: Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Ratchathewi, Bangkok, 10400, Thailand. am_sumate@hotmail.com.
PMID: 37495626 DOI: 10.1038/s41598-023-39218-y

Abstract

Therapeutic treatment forms can play significant roles in resolving psoriatic plaques or promoting wound repair in psoriatic skin. Considering the biocompatibility, mechanical strength, flexibility, and adhesive properties of silk fibroin sheets/films, it is useful to combine them with anti-psoriatic agents and healing stimulants, notably silk sericin. Here, we evaluate the curative properties of sericin-coated thin polymeric films (ScF) fabricated from silk fibroin, using an imiquimod-induced psoriasis rat model. The film biocompatibility and psoriatic wound improvement capacity was assessed. A proteomics study was performed to understand the disease resolving mechanisms. Skin-implantation study exhibited the non-irritation property of ScF films, which alleviate eczema histopathology. Immunohistochemical and gene expression revealed the depletion of β-defensin, caspase-3 and -9, TNF-α, CCL-20, IL-1β, IL-17, TGF-β, and Wnt expressions and S100a14 mRNA level. The proteomics study suggested that ScF diminish keratinocyte proliferation via the mTOR pathway by downregulating mTOR protein, corresponding to the modulation of TNF-α, Wnt, and IL-1β levels, leading to the enhancement of anti-inflammatory environment by IL-17 downregulation. Hematology data demonstrated the safety of using these biomaterials, which provide a potential therapeutic-option for psoriasis treatment due to desirable effects, especially anti-proliferation and anti-inflammation, functioning via the mTOR pathway and control of IL-17 signaling.

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MeSH Term

Rats
Animals
Sericins
Interleukin-17
Tumor Necrosis Factor-alpha
Fibroins
Psoriasis
Skin
Inflammation
Anti-Inflammatory Agents
TOR Serine-Threonine Kinases
Polymers
Keratinocytes

Chemicals

Sericins
Interleukin-17
Tumor Necrosis Factor-alpha
Fibroins
Anti-Inflammatory Agents
TOR Serine-Threonine Kinases
Polymers
mTOR protein, rat
Journal Article Research Support, Non-U.S. Gov't

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