Recellularization of Acellular Xeno Kidney Scaffold: An In Vivo Method to Generate Bioartificial Kidney. - National Genomics Data Center (CNCB-NGDC)

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Recellularization of Acellular Xeno Kidney Scaffold: An In Vivo Method to Generate Bioartificial Kidney.

Kishor Tardalkar, Nilesh Bhamare, Jeevitaa Kshersagar, Leena Chaudhari, Nimish Deshpande, Jitendra Patil, Rakesh Kumar Sharma, Meghnad G Joshi

Author Information

Kishor Tardalkar: Department of Stem Cells & Regenerative Medicine, D Y Patil Education Society (Deemed to be University), Kolhapur, Maharashtra, India.
Nilesh Bhamare: Department of Stem Cells & Regenerative Medicine, D Y Patil Education Society (Deemed to be University), Kolhapur, Maharashtra, India.
Jeevitaa Kshersagar: Department of Stem Cells & Regenerative Medicine, D Y Patil Education Society (Deemed to be University), Kolhapur, Maharashtra, India.
Leena Chaudhari: Department of Stem Cells & Regenerative Medicine, D Y Patil Education Society (Deemed to be University), Kolhapur, Maharashtra, India.
Nimish Deshpande: Department of Stem Cells & Regenerative Medicine, D Y Patil Education Society (Deemed to be University), Kolhapur, Maharashtra, India.
Jitendra Patil: Department of Radiology, D Y Patil Medical College, D Y Patil Education Society (Deemed to be University), Kolhapur, Maharashtra, India.
Rakesh Kumar Sharma: Department of Obstetrics and Gynaecology, D Y Patil Medical College, D Y Patil Education Society (Deemed to be University), Kolhapur, Maharashtra, India.
Meghnad G Joshi: Department of Stem Cells & Regenerative Medicine, D Y Patil Education Society (Deemed to be University), Kolhapur, Maharashtra, India.

PMID: 37610657 DOI: 10.1007/5584_2023_785

A significant hurdle for kidney tissue engineering is reproducing the complex three-dimensional structure of the kidney. In our study, a stepwise approach of generating a reproducible Xeno kidney scaffold from a goat kidney is described, which can be implanted and recellularized by host cells. We have proposed a combination of sodium dodecyl sulfate and Triton-X-100-based protocol to generate a reproducible Xeno kidney scaffold, which was then analyzed by histology, DNA quantification, SEM, and renal angiography. Further, a small portion from the cortico-medullar region of the acellular scaffold was implanted in the rat's kidney subcapsular pocket for a period of 1 month, to check the recruitment of host cells into the scaffold. Post implantation, the extracellular matrix of the scaffold was well preserved and it did not induce any damage or inflammation in the native kidney. Implantation of the Xeno scaffold resulted in apparent early vascularization which helped in the recruitment of the host cells, which was characterized by histology, immunohistochemistry, and scanning electron microscopy. Implanted Xeno scaffold showed AQP-1, Nephrin, α-SMA, and VEGF expression in proximal tubules and renal glomerulus. Importantly, Ki-67 and WTAP-expressing cells were also observed near proximal tubules suggesting a high level of proliferation in the scaffold. Thus, showing the potential of Xeno kidney development that can be recellularized by the host cell to engineer into a functional kidney.

Extracellular matrix Glomerulus In vivo recellularization Kidney tissue engineering Xeno kidney scaffold

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Rats

Animals

Tissue Scaffolds

Tissue Engineering

Extracellular Matrix

Kidney

DNA

DNA

Journal Article