Jianyu Lu, Zhenhui Wang, Jinxi Li, Qian Zhao, Fan Qi, Fu Wang, Chunxiao Xiaoyang, Guofei Tan, Hanlu Wu, Michael K Deyholos, Ningning Wang, Yingnan Liu, Jian Zhang
Flax is an important cash crop globally with a variety of commercial uses. It has been widely used for fiber, oil, nutrition, feed and in composite materials. Growth regulatory factor (GRF) is a transcription factor family unique to plants, and is involved in regulating many processes of growth and development. Bioinformatics analysis of the GRF family in flax predicted 17 genes, which all contained the characteristic QLQ and WRC domains. Equally, 15 of 17 (88%) are predicted to be regulated by lus-miR396 miRNA. Phylogenetic analysis of GRFs from flax and several other well-characterized species defined five clades; genes were found in four clades. Most gene promoters contained cis-regulatory elements known to be responsive to hormones and stress. The chromosomal locations and collinearity of genes were also analyzed. The three-dimensional structure of proteins was predicted using homology modeling. The transcript expression data indicated that most family members were highly expressed in flax fruit and embryos, whereas , and were enriched in response to salt stress. Real-time quantitative fluorescent PCR (qRT-PCR) showed that both and were up-regulated under ABA and MeJA stimuli, indicating that these genes were involved in defense. was demonstrated to be localized to the nucleus as expected for a transcription factor. These results provide a basis for further exploration of the molecular mechanism of gene function and obtaining improved flax breeding lines.
Plant Physiol. 2018 Apr;176(4):2963-2976
[PMID:
29439209]
Plant Physiol. 2014 Jan;164(1):249-58
[PMID:
24285851]
Plant Physiol. 2014 Apr;164(4):1952-66
[PMID:
24532604]
PeerJ. 2021 Apr 8;9:e11235
[PMID:
33889451]
Int J Mol Sci. 2019 Oct 21;20(20):
[PMID:
31640280]
Nat Methods. 2015 Apr;12(4):357-60
[PMID:
25751142]
PLoS One. 2023 May 10;18(5):e0285494
[PMID:
37163544]
Nat Plants. 2015 Dec 21;2:15203
[PMID:
27250749]
Plant Cell Physiol. 2004 Jul;45(7):897-904
[PMID:
15295073]
Genet Mol Biol. 2020 Jul 24;43(3):20200080
[PMID:
32706846]
Front Plant Sci. 2020 Oct 12;11:572319
[PMID:
33154762]
Development. 2010 Jan;137(1):103-12
[PMID:
20023165]
Physiol Mol Biol Plants. 2022 Sep;28(9):1717-1735
[PMID:
36387975]
Front Plant Sci. 2023 Apr 14;14:1159223
[PMID:
37123828]
Mol Plant. 2015 Oct 5;8(10):1455-65
[PMID:
26187814]
Plant Signal Behav. 2022 Dec 31;17(1):2041280
[PMID:
35318879]
Plant Sci. 2023 Jan;326:111502
[PMID:
36252856]
Plant J. 2003 Oct;36(1):94-104
[PMID:
12974814]
Biometals. 2021 Jun;34(3):639-660
[PMID:
33783656]
Mol Plant. 2015 Jul;8(7):998-1010
[PMID:
25620770]
Plant Biol (Stuttg). 2013 Sep;15(5):892-8
[PMID:
23173976]
Int J Mol Sci. 2018 Oct 17;19(10):
[PMID:
30336563]
Plant Physiol. 2014 May;165(1):160-74
[PMID:
24596329]
Sci Rep. 2018 Sep 7;8(1):13447
[PMID:
30194309]
Plants (Basel). 2021 Sep 15;10(9):
[PMID:
34579449]
Plant Cell. 2012 Aug;24(8):3393-405
[PMID:
22942381]
PLoS Genet. 2018 Jul 9;14(7):e1007484
[PMID:
29985961]
Nucleic Acids Res. 2015 Apr 20;43(7):e47
[PMID:
25605792]
Plants (Basel). 2022 Aug 05;11(15):
[PMID:
35956530]
Bioinformatics. 2018 Sep 1;34(17):i884-i890
[PMID:
30423086]
Plant Physiol. 2000 Mar;122(3):695-704
[PMID:
10712532]
Int J Mol Sci. 2022 Jun 15;23(12):
[PMID:
35743113]
Plant Physiol. 2012 May;159(1):321-35
[PMID:
22419826]
BMC Plant Biol. 2019 Jun 21;19(1):269
[PMID:
31226949]
Funct Integr Genomics. 2017 Sep;17(5):503-512
[PMID:
28236273]
Int J Mol Sci. 2017 May 13;18(5):
[PMID:
28505092]
Front Genet. 2022 Feb 03;12:727674
[PMID:
35185998]
Nat Rev Genet. 2009 May;10(5):305-17
[PMID:
19360022]
Plant J. 2005 Jul;43(1):68-78
[PMID:
15960617]
Mol Plant. 2023 Nov 6;16(11):1733-1742
[PMID:
37740491]
BMC Plant Biol. 2010 Apr 19;10:71
[PMID:
20403198]
Bioinformatics. 2010 Jan 1;26(1):139-40
[PMID:
19910308]
Nucleic Acids Res. 2019 May 7;47(8):e47
[PMID:
30783653]
Front Plant Sci. 2016 Apr 06;7:399
[PMID:
27092149]
Plant Sci. 2016 Jun;247:1-12
[PMID:
27095395]
BMB Rep. 2019 Apr;52(4):227-238
[PMID:
30885290]
Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13374-9
[PMID:
15326298]
BMC Genomics. 2020 Aug 24;21(1):575
[PMID:
32831017]
Nat Plants. 2015 Dec 21;2:15195
[PMID:
27250747]
BMC Genomics. 2023 Jun 16;24(1):334
[PMID:
37328802]
J Exp Bot. 2012 Jun;63(10):3727-40
[PMID:
22442419]
Mol Biol Rep. 2016 Oct;43(10):1059-67
[PMID:
27491940]
Plant J. 2014 Aug;79(3):413-26
[PMID:
24888433]
Front Plant Sci. 2023 Mar 01;14:1112354
[PMID:
36938021]
BMC Bioinformatics. 2021 Feb 1;22(1):41
[PMID:
33526006]
Plant Cell. 2013 Sep;25(9):3570-83
[PMID:
24076976]
Res Vet Sci. 2013 Aug;95(1):103-9
[PMID:
23465779]
PLoS One. 2014 May 29;9(5):e98477
[PMID:
24875638]
BMC Plant Biol. 2020 May 8;20(1):200
[PMID:
32384927]
Nutrients. 2020 Oct 16;12(10):
[PMID:
33081119]
