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Oral diseases
09, 2024;30 (6): 3897-3911.

Comparative analysis of two arecoline-induced oral submucous fibrosis models.

Shijie Tang, Lanxin Jiang, Ying Zhou, Tong Zhou, Yang Peng, Shuting Zhou, Yuan Yue, Xiaoqiang Xia, Jing Li, Qianming Chen, Yuchen Jiang, Xiaodong Feng
Author Information
  1. Shijie Tang: State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, China. ORCID
  2. Lanxin Jiang: State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
  3. Ying Zhou: State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
  4. Tong Zhou: State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
  5. Yang Peng: State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
  6. Shuting Zhou: State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
  7. Yuan Yue: State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, China. ORCID
  8. Xiaoqiang Xia: State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
  9. Jing Li: State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
  10. Qianming Chen: Key Laboratory of Oral Biomedical Research of Zhejiang Province, Affiliated Stomatology Hospital, Zhejiang University School of Stomatology, Hangzhou, China.
  11. Yuchen Jiang: State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, China. ORCID
  12. Xiaodong Feng: State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, China. ORCID
PMID: 38098250 DOI: 10.1111/odi.14825

Abstract

OBJECTIVE: The limited understanding of the molecular mechanism for oral submucosal fibrosis (OSF) poses challenges to the development of effective prevention and treatment strategies. The lack of suitable animal models is a major hindrance. Therefore, this study aimed to address this issue by comparing commonly used arecoline-induced water drinking and injection mouse models.
MATERIALS AND METHODS: The mice were subjected to two protocols: receiving 2 mg/mL arecoline in drinking water and 4 mg/mL arecoline saline solution injections every other day. Tissues were collected at regular 4-week intervals, with a final time point of 20 weeks. Stereo microscopy and histomorphological analysis were performed on live and harvested tissues, respectively.
RESULTS: During arecoline treatment, collagen deposition and myofibroblast proliferation progressively increased in both models. Changes in the collagen I/III ratio indicated that both models exhibited characteristics of the early and intermediate stages of OSF after 20 weeks of arecoline induction. The water-drinking model also demonstrated multi-organ fibrosis involving the tongue, lungs, and small intestine.
CONCLUSION: Both the water drinking and injection mouse models effectively induced OSF, but the water-drinking model better mirrored the observed pathogenesis in patients with OSF. These models provide valuable tools for investigating the mechanisms underlying OSF.

Keywords

arecoline mouse models oral potentially malignant disorders oral submucous fibrosis

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Grants

  1. 2019-I2M-5-004/CAMS Innovation Fund for Medical Sciences
  2. YJ201987/Fundamental Research Funds for the Central Universities
  3. QDJF2019-3/Scientific Research Foundation, West China Hospital of Stomatology Sichuan University
  4. RD-03-202110/Scientific Research Foundation, West China Hospital of Stomatology Sichuan University
  5. RCDWJS2021-8/Scientific Research Foundation, West China Hospital of Stomatology Sichuan University
  6. 2021ZYD0090/Sichuan Province Science and Technology Support Program
  7. 2022YFS0207/Sichuan Province Science and Technology Support Program
  8. 82170971/National Natural Science Foundations of China
  9. 82002888/National Natural Science Foundations of China

MeSH Term

Animals
Arecoline
Oral Submucous Fibrosis
Disease Models, Animal
Mice
Tongue
Myofibroblasts
Male
Intestine, Small
Lung
Collagen Type I
Cell Proliferation
Collagen Type III

Chemicals

Arecoline
Collagen Type I
Collagen Type III
Journal Article Comparative Study Research Support, Non-U.S. Gov't

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