Establishing Air-Liquid Interface (ALI) Airway Culture Models for Infectious Disease Research.

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Kim R Chiok, Nuraina A Dahlan, Arinjay Banerjee, Neeraj Dhar

Author Information

Kim R Chiok: Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada.
Nuraina A Dahlan: Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada.
Arinjay Banerjee: Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada.
Neeraj Dhar: Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada. neeraj.dhar@usask.ca.

PMID: 38888776 DOI: 10.1007/978-1-0716-3890-3_10

Air-liquid interface (ALI) airway culture models serve as a powerful tool to emulate the characteristic features of the respiratory tract in vitro. These models are particularly valuable for studying emerging respiratory viral and bacterial infections. Here, we describe an optimized protocol to obtain the ALI airway culture models using normal human bronchial epithelial cells (NHBECs). The protocol outlined below enables the generation of differentiated mucociliary airway epithelial cultures by day 28 following exposure to air.

Air-liquid interface (ALI) airway culture Host-pathogen interaction In vitro models

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Humans

Cell Culture Techniques

Epithelial Cells

Bronchi

Respiratory Mucosa

Air

Cells, Cultured

Communicable Diseases

Journal Article

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