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08 24, 2024;16 (9):

The First Isolation of Insect-Specific Alphavirus () in Culex (Melanoconion) Mosquitoes in the Brazilian Amazon.

Bruna Ramos, Valéria Carvalho, Eliana da Silva, Maria Freitas, Landeson Junior Barros, Maissa Santos, Jamilla Augusta Pantoja, Ercília Gonçalves, Joaquim Nunes Neto, José Wilson Junior, Durval Vieira, Daniel Dias, Ana Cecília Cruz, Bruno Nunes, Sandro Silva, Carine Aragão, Alexandre Casseb, Lívia Martins
Author Information
  1. Bruna Ramos: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, Km 07, s/n, Ananindeua 67030-000, PA, Brazil. ORCID
  2. Valéria Carvalho: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, Km 07, s/n, Ananindeua 67030-000, PA, Brazil. ORCID
  3. Eliana da Silva: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, Km 07, s/n, Ananindeua 67030-000, PA, Brazil. ORCID
  4. Maria Freitas: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, Km 07, s/n, Ananindeua 67030-000, PA, Brazil. ORCID
  5. Landeson Junior Barros: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, Km 07, s/n, Ananindeua 67030-000, PA, Brazil.
  6. Maissa Santos: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, Km 07, s/n, Ananindeua 67030-000, PA, Brazil.
  7. Jamilla Augusta Pantoja: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, Km 07, s/n, Ananindeua 67030-000, PA, Brazil.
  8. Ercília Gonçalves: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, Km 07, s/n, Ananindeua 67030-000, PA, Brazil.
  9. Joaquim Nunes Neto: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, Km 07, s/n, Ananindeua 67030-000, PA, Brazil. ORCID
  10. José Wilson Junior: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, Km 07, s/n, Ananindeua 67030-000, PA, Brazil.
  11. Durval Vieira: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, Km 07, s/n, Ananindeua 67030-000, PA, Brazil.
  12. Daniel Dias: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, Km 07, s/n, Ananindeua 67030-000, PA, Brazil.
  13. Ana Cecília Cruz: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, Km 07, s/n, Ananindeua 67030-000, PA, Brazil. ORCID
  14. Bruno Nunes: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, Km 07, s/n, Ananindeua 67030-000, PA, Brazil. ORCID
  15. Sandro Silva: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, Km 07, s/n, Ananindeua 67030-000, PA, Brazil. ORCID
  16. Carine Aragão: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, Km 07, s/n, Ananindeua 67030-000, PA, Brazil. ORCID
  17. Alexandre Casseb: Institute of Animal Health and Production, Federal Rural University of Amazônia, President Tancredo Neves Boulevard, 2501, Belem 66077-830, PA, Brazil.
  18. Lívia Martins: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, Km 07, s/n, Ananindeua 67030-000, PA, Brazil. ORCID
PMID: 39339832 DOI: 10.3390/v16091355

Abstract

Advances in diagnostic techniques coupled with ongoing environmental changes have resulted in intensified surveillance and monitoring of arbovirus circulation in the Amazon. This increased effort has resulted in increased detection of insect-specific viruses among hematophagous arthropods collected in the field. This study aimed to document the first isolation of in mosquitoes collected within the Brazilian Amazon. Arthropods belonging to the family Culicidae were collected within a forest fragment located in the Environmental Protection Area of the metropolitan region of Belem. Subsequently, these specimens were meticulously identified to the species level. Afterward, the collected batches were macerated, and the resulting supernatant was then inoculated into C6/36 and Vero cell cultures to facilitate viral isolation. The presence of arboviruses within the inoculated cell cultures was determined through indirect immunofluorescence analysis. Furthermore, positive supernatant samples underwent nucleotide sequencing to precisely identify the viral strains present. Notably, a batch containing () mosquitoes was identified to be positive for the genus via indirect immunofluorescence. This study is the first report on insect-specific alphavirus isolation in Brazil and the first-ever description of isolation within Amazon Forest remnants.

Keywords

Agua Salud alphavirus Amazon insect-specific viruses mosquitoes

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Grants

  1. CNPq-process number 141723/2018-1/National Council for Scientific and Technological Development
  2. 141723/2018-1/National Council for Scientific and Technological Development

MeSH Term

Animals
Alphavirus
Brazil
Vero Cells
Chlorocebus aethiops
Culex
Mosquito Vectors
Phylogeny
Arboviruses
Journal Article Research Support, Non-U.S. Gov't

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