Alastair Taylor, Sylvia Blum, Madeleine Ball, Owen Birch, Hsuan Chou, Julia Greenwood, Shane Swann, Lara Pocock, Max Allsworth, Billy Boyle, Kerstin Geillinger-Kaestle
A new pre-clinical method for capturing breath samples from intubated mice is presented. This method significantly reduces background levels, allowing more accurate measurements of VOCs originating from the breath ("on-breath") as opposed to background contamination. The method was developed by integrating industry-standard volatile-capturing sorbent tubes with respiratory mechanics measurement equipment (flexiVent), resulting in a mouse breath sample that can be transported and analyzed by TD-GC-MS and other central lab technologies. Using the methodology, the discrimination between on-breath VOCs from background compounds provides a cleaner dataset, which can accelerate the validation of VOCs identified from mouse models and their translation to clinical trials. Three metrics were developed to identify on-breath VOCs, with 22 identified using Type 1 (50% of the breath samples exceeding three standard deviations above the mean signal of the system blanks), 34 with Type 2 (-value ≤ .05 between paired breath and blank samples), and 61 with Type 3 (ROC-AUC value ≥ 0.8 to differentiate between breath and blank samples). The number of compounds seen at elevated levels on mouse breath was quantified and compared to the levels seen on human breath samples to compare methodologies.
Multidiscip Respir Med. 2020 Feb 19;15(1):36
[PMID:
32269772]
World J Gastroenterol. 2014 Sep 28;20(36):12847-59
[PMID:
25278682]
J Clin Med. 2020 Dec 24;10(1):
[PMID:
33374433]
J Breath Res. 2022 Feb 14;16(2):
[PMID:
35086080]
Thorax. 2011 Sep;66(9):804-9
[PMID:
21749985]
Microbiome. 2021 Jul 8;9(1):157
[PMID:
34238386]
Biotechnol Adv. 2011 May-Jun;29(3):351-64
[PMID:
21272631]
Front Pediatr. 2019 Mar 19;7:68
[PMID:
30941334]
J Breath Res. 2020 May 27;14(3):034001
[PMID:
32163929]
Am J Respir Crit Care Med. 2019 Aug 15;200(4):444-453
[PMID:
30973757]
Allergy Asthma Clin Immunol. 2021 Nov 27;17(1):121
[PMID:
34838143]
J Chromatogr A. 2013 Aug 2;1301:179-89
[PMID:
23764192]
J Breath Res. 2022 Feb 23;16(2):
[PMID:
35120340]
Curr Drug Metab. 2005 Jun;6(3):227-40
[PMID:
15975041]
Gut. 1985 Aug;26(8):834-42
[PMID:
4018650]
Am J Respir Crit Care Med. 2024 Nov 1;210(9):1101-1112
[PMID:
38820123]
J Breath Res. 2024 Feb 12;18(2):
[PMID:
38290132]
Metabolites. 2019 Mar 18;9(3):
[PMID:
30889835]
Molecules. 2022 Apr 07;27(8):
[PMID:
35458579]
JAMA Oncol. 2019 Jan 1;5(1):e182815
[PMID:
30128487]
J Breath Res. 2014 Sep;8(3):034001
[PMID:
24946087]
J Breath Res. 2017 Dec 06;12(1):016007
[PMID:
29211685]
Metabolites. 2022 Dec 21;13(1):
[PMID:
36676933]
Am J Gastroenterol. 2017 May;112(5):775-784
[PMID:
28323273]
Metabolomics. 2024 Jul 8;20(4):72
[PMID:
38977623]
J Breath Res. 2014 Sep;8(3):037101
[PMID:
25189420]
Molecules. 2020 Dec 10;25(24):
[PMID:
33321824]
Nat Rev Drug Discov. 2017 Jun;16(6):381-382
[PMID:
28529317]
Am J Respir Crit Care Med. 2024 Aug 1;210(3):288-297
[PMID:
38635834]
Metabolomics. 2024 Sep 6;20(5):102
[PMID:
39242444]
EBioMedicine. 2015 Jul 26;2(9):1243-50
[PMID:
26501124]
Biomedicines. 2023 Nov 01;11(11):
[PMID:
38001958]
EBioMedicine. 2020 Apr;54:102725
[PMID:
32330874]
J Breath Res. 2023 Apr 19;17(3):
[PMID:
37001512]
Neurotoxicol Teratol. 2013 Mar-Apr;36:82-90
[PMID:
22975621]
J Vis Exp. 2013 May 15;(75):e50172
[PMID:
23711876]
J Anal Toxicol. 2024 Mar 28;48(3):171-179
[PMID:
38334750]
J Breath Res. 2023 May 03;17(3):
[PMID:
37074671]
Anesth Analg. 2019 Oct;129(4):e126-e129
[PMID:
30489316]
Diagnostics (Basel). 2019 Dec 17;9(4):
[PMID:
31861135]
J Breath Res. 2021 Apr 21;15(3):
[PMID:
33761469]
J Breath Res. 2014 Mar;8(1):016004
[PMID:
24566092]
J Proteome Res. 2014 Dec 5;13(12):5281-92
[PMID:
25245235]
