Sorghum virgatum (Sv) is a wild subspecies of sorghum (Sorghum bicolor ssp. Verticilliflorum (L.) Moench). We employed Hi-C sequencing and HiFi technology in this study, successfully assembly a high-quality genome for Sv. This assembled genome is 795 Mb in total and scaffold N50 is 62.47 Mb. Within the Sv genome, 27,851 protein-coding genes were predicted. Repeat annotation detected 563.96 Mb of repetitive elements, making up 71.86% of the genome. This chromosome-level sv genome offers valuable insights to support comparative genomic studies within the Poaceae family, and it will aid genome-driven breeding and germplasm enhancement for modern sorghum.
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