RiceWiki - User contributions [en]

Os01g0128300

2014-12-03T13:37:42Z

潘文波:

Please input one-sentence summary here.

==Annotated Information==
===Function===
[[File:J003.png|right|thumb|350px|''MHZ4 expression and protein subcellular localization.<ref name="ref1" />'']]
The MHZ4 gene encodes a protein of 228 amino acids that harbors an N-terminal signal peptide and four transmembrane domains as predicted by the SMART program (http://smart.embl-heidelberg.de/) (Figure 2B). The MHZ4 sequence shared 47% identity and 64% similarity with Arabidopsis ABA4 (AT1G67080), which is required for neoxanthin formation in the ABA biosynthesis pathway through an unknown mechanism [32].
===Expression===
MHZ4 accumulation was examined by semiquantitative RT-PCR. The transcripts were detected in all organs from vegetative to reproductive stages and found to be more abundant in young leaves (Figure 3A). Transgenic rice plants harboring MHZ4 promoter::β-glucuronidase (GUS) construct were also generated and GUS staining assay was performed to evaluate the promoter activity. In etiolated seedlings, MHZ4 expression was detected in both roots and coleoptiles, and the signals were also present in the vascular tissues of roots (Figure 3B, a to e). In root apexes, GUS signals were observed in the putative quiescent center (QC) and root caps (Figure 3B, f). In field-grown plants, MHZ4 was expressed in leaf blades, young stem nodes, the base of axillary buds and adventitious roots derived from the nodes (Figure 3B, g to j). In reproductive organs, the expression of MHZ4 was detected in the anthers and pistil of young flowers, lemma of mature flowers, and parts of developing grains (Figure 3B, k to n).

===Evolution===
[[File:1.png|right|thumb|350px|''Phylogenetic analysis revealed that MHZ4 .<ref name="ref1" />'']]
Phylogenetic analysis revealed that MHZ4 protein is conserved from cyanobacteria to higher plants and is more closely related to homologues from monocotyledonous plants (Figure 2E).

===Ethylene Response Phenotypes===
[[File:g001.png|left|thumb|350px|. ''Ethylene responses in etiolated mhz4 seedlings..<ref name="ref1" />'']]

The mhz4 is identified previously in our screen for ethylene-response mutants in rice [45]. For dark-grown wild type (WT) seedlings, ethylene inhibited root growth but promoted coleoptile elongation in a dose-dependent manner (Figure 1A–C). The roots of etiolated mhz4 seedlings were about 10% shorter than that of WT seedlings under normal conditions. Upon ethylene treatment, mhz4 roots were insensitive to ethylene inhibition at lower concentrations (≤1 ppm) but displayed mild growth inhibition at higher concentrations (10 to 100 ppm), e.g. about 20% inhibition in mhz4 compared with about 70% inhibition in the WT at 10 ppm ethylene (Figure 1A and 1B). This indicates that the roots of mhz4 are less sensitive to ethylene. On the other hand, the coleoptiles of mhz4 were slightly but significantly (P<0.004) longer than that of WT seedlings in the absence of ethylene and were much longer than that of WT under all concentrations of ethylene treatment, indicating that the coleoptiles of mhz4 are hypersensitive to ethylene (Figure 1A and 1C). These results indicate that mhz4 mutation oppositely affects ethylene responses in roots and coleoptiles of etiolated rice seedlings.

To further confirm the ethylene responsiveness of mhz4 mutant, we examined expressions of ethylene-inducible genes originally identified from a chip analysis (GSE51153; [45]) and a RNA-seq analysis (SRP041468). Two genes including disease resistance response gene and peptidylprolyl isomerase gene were found to be induced by ethylene in both shoots and roots of WT seedlings (Figure 1D). Four genes (OsERF002, OsRRA5, OsRAP2.8 and OsIAA20) were found to be induced mainly in roots and four genes (SHR5, OsERF063, OsERF073 and photosystem II 10 kDa polypeptide gene) mainly in shoots of WT seedlings (Figure 1E). In mhz4 shoots, the ethylene inducible genes were constitutively expressed at a level higher than that in ethylene-treated WT. Ethylene treatment of mhz4 did not further increase the transcript levels probably due to their expression levels were already very high. It should be noted that we used shoots instead of coleoptiles for the gene expression analyses because similar ethylene responses were found for coleoptiles and shoots [46]. In mhz4 roots, the transcripts of ethylene inducible genes remained at a similar or lower level when compared to those in WT in the absence of ethylene, and these genes showed no or only slight inductions compared to their ethylene inductions in WT roots (Figure 1D, E). These results indicate enhanced and reduced ethylene response in mhz4 shoots/coleoptiles and roots, respectively, at gene expression level

==Labs working on this gene==
1.Biao Ma, Cui-Cui Yin, Si-Jie He, Xiang Lu, Wan-Ke Zhang, Shou-Yi Chen, Jin-Song Zhang
State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
2.Tie-Gang Lu
Biotechnology Research Institute/National Key Facility for Genetic Resources and Gene Improvement, Chinese Academy of Agricultural Sciences, Beijing, China
==References==
1. Biao Ma , Cui-Cui Yin , Si-Jie He, Xiang Lu, Wan-Ke Zhang, Tie-Gang Lu, Shou-Yi Chen mail, Jin-Song Zhang .Ethylene-Induced Inhibition of Root Growth Requires Abscisic Acid Function in Rice (Oryza sativa L.) Seedlings. plos genetics. DOI: 10.1371/journal.pgen.1004701

==Structured Information==
{{JaponicaGene|
GeneName = Os01g0128300|
Description = Conserved hypothetical protein|
Version = NM_001048446.2 GI:297596022 GeneID:4325995|
Length = 1631 bp|
Definition = Oryza sativa Japonica Group Os01g0128300, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:1568258..1569888|
CDS = 1568258..1568296,1568775..1568880,1568994..1569082,1569162..1569290,1569377..1569477 ,1569577..1569769,1569853..1569888|
GCID = <gbrowseImage1>
name=NC_008394:1568258..1569888
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:1568258..1569888
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggctctcctcctcctctcctccgccgctcgggttggcgtggctgcgccactggcgctgaggcagcagcgcccggtggtgctgcccggtggccagctccgcacgggaagcggcgccggcgcagcgtcggcgtgggcggcgcgccctctccggccggagctcgccgcggtctcccgccccgccgtccccgcccgcggcagggcgcctctgttccggcctcgcgcatggatggcgtcgtctcagattgccagctccgccttcacctggggcaccatcgccgtcctccctttctacactctcatggtcgtcgcccccaacgccgacgtcaccaagcgtgccgtggatagcagcgcgccgtacgtcgccctcggcatcctctacgcctacctgctctacctatcctggactcccgacaccctacgcgccatgttcgccagcaagtactggctccccgagttgacggggatcgtcaggatgttcgccagcgagatgaccgtcgcctccgcctggatccacctcctagccgtcgacctcttcgccgcaaggcaggtgtaccatgacggcatcaagaacaacatagagaccaggcattcggtttctctgtgcctgctcttctgcccaattgggatcgcgactcacgttctgactaaggcctatgtgcttcttacaaataaatgtgctttccaataa</cdnaseq>|
AA = <aaseq>MAALLLLSSAARVGVAAPLALRQQRPVVLPGGQLRTGSGAGAAS AWAARPLRPELAAVSRPAVPARGRAPLFRPRAWMASSQIASSAFTWGTIAVLPFYTLM VVAPNADVTKRAVDSSAPYVALGILYAYLLYLSWTPDTLRAMFASKYWLPELTGIVRM FASEMTVASAWIHLLAVDLFAARQVYHDGIKNNIETRHSVSLCLLFCPIGIATHVLTK AYVLLTNKCAFQ</aaseq>|
DNA = <dnaseqindica>1593..1631#1009..1114#807..895#599..727#412..512#120..312#1..36#atggcggctctcctcctcctctcctccgccgctcgggtacgtagccggtaacacatgactcagtcaatcttggcacttgcggagttgcggtagcttcagttcttgatttgctgctgcaggttggcgtggctgcgccactggcgctgaggcagcagcgcccggtggtgctgcccggtggccagctccgcacgggaagcggcgccggcgcagcgtcggcgtgggcggcgcgccctctccggccggagctcgccgcggtctcccgccccgccgtccccgcccgcggcagggcgcctctgttccggcctcgcgcatgtaacgcctctgcggccccattcaattctagctctaccacaactgattggtgtaggactgactgacgaatttcgccatggatttggttgtgaggagcagggatggcgtcgtctcagattgccagctccgccttcacctggggcaccatcgccgtcctccctttctacactctcatggtcgtcgcccccaacgccgacgtcgtaagaattcctcaatcctccaacccacacacacacaatcgaattgctctcacttgctcctctaatctttctctgtgctggcgcagaccaagcgtgccgtggatagcagcgcgccgtacgtcgccctcggcatcctctacgcctacctgctctacctatcctggactcccgacaccctacgcgccatgttcgccagcaagtactggctccccgaggtcagtcaatcaatcagtgacaccactctgcactccatgatcactagcattctctcaacctttctcttctcctgcgcagttgacggggatcgtcaggatgttcgccagcgagatgaccgtcgcctccgcctggatccacctcctagccgtcgacctcttcgccgcaaggtgagcaaattcttgattcatgtgtgtagtagtgtacctatggatgatggttgtatgtgttgcaagcgtgaggaggttgttaaaatggcttgagttttgggatcacaatgcaggcaggtgtaccatgacggcatcaagaacaacatagagaccaggcattcggtttctctgtgcctgctcttctgcccaattgggatcgcgactcacgttctgactaaggtactggcgggttcaattggtcgctcacattgatcgattgtatgtaggttcatattgcttagatggtgtatttttatgtgattcatatatgaataaaatttattcaattaactatgtgttacatcgagttcaatgtgattcttgtttttccccacgtattgaatcgatttggcagctgaacatccctataccggtttagcgtactctactttctcagtagctaatgaagaaaagtgcaattgacaatatgatcagagataagcgacaactcttaaatgtttgcaaataaagaaatgagatgattagagggaaatgaatcgatttgaaacacacttattcaagtggtacactgttcaaacatagaggaattacaacaattacatttatgaatgctatactaggcttatgtgtttcttgtcacagactactttcaattgtacatgaatgacagcatttacagttttgaatgctgtactaggcctatgtgcttcttacaaataaatgtgctttccaataa</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001048446.2 RefSeq:Os01g0128300]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

File:G001.png

2014-12-03T13:33:16Z

潘文波:

File:1.png

2014-12-03T13:29:09Z

潘文波: uploaded a new version of "File:1.png"

[[File:1.png]]

File:J003.png

2014-12-03T13:25:57Z

潘文波:

Os03g0719100

2014-06-26T12:37:12Z

潘文波:

Please input one-sentence summary here.

==Annotated Information==
===Function===
[[File:PCE 2195 f6.gif|right|thumb|300px|'''Figure 1a.''' Complementation of OsSIZ1 and OsSIZ2 and their corresponding expression levels in siz1 mutant plants.]]
[[File:PCE 2195 f7.gif|right|thumb|300px|'''Figure 1b.''' Suppression of ABA-hypersensitivity of siz1-2 by complementation of OsSIZ1 and OsSIZ2.]]

Sumoylation is a post-translational regulatory process in diverse cellular processes in eukaryotes, involving conjugation/deconjugation of small ubiquitin-like modifier (SUMO) proteins to other proteins thus modifying their function. The PIAS [protein inhibitor of activated signal transducers and activators of transcription (STAT)] and SAP (scaffold attachment factor A/B/acinus/PIAS)/MIZ (SIZ) proteins exhibit SUMO E3-ligase activity that facilitates the conjugation of SUMO proteins to target substrates. Here, we report the isolation and molecular characterization of Oryza sativa SIZ1 (OsSIZ1) and SIZ2 (OsSIZ2), rice homologs of Arabidopsis SIZ1. The rice SIZ proteins are localized to the nucleus and showed sumoylation activities in a tobacco system. Our analysis showed increased amounts of SUMO conjugates associated with environmental stresses such as high and low temperature, NaCl and abscisic acid (ABA) in rice plants. The expression of OsSIZ1 and OsSIZ2 in siz1-2 Arabidopsis plants partially complemented the morphological mutant phenotype and enhanced levels of SUMO conjugates under heat shock conditions. In addition, ABA-hypersensitivity of siz1-2 seed germination was partially suppressed by OsSIZ1 and OsSIZ2. The results suggest that rice SIZ1 and SIZ2 are able to functionally complement Arabidopsis SIZ1 in the SUMO conjugation pathway. Their effects on the Arabidopsis mutant suggest a function for these genes related to stress responses and stress adaptation.

===Expression===
[[File:1234235.png|left|thumb|300px|'''Figure 2.''' Expression of OsSIZ1 and OsSIZ2 by RT-PCR analysis .]]
To determine whether OsSIZ1 and OsSIZ2 show tissue-specific expression, we analyzed their expression levels in various rice tissues. As shown in Fig. 2, transcripts of OsSIZ1 and OsSIZ2 were detected in all tissues of rice, but to a lesser extent in root. Next, we investigated whether the expression of OsSIZ genes could be induced in response to abiotic, biotic, and hormone stimuli. However, none of these treatments induced transcription of the OsSIZ genes

[[File:45y644t.gif|left|thumb|300px|'''Figure 4.''' Subcellular localization of OsSIZ1 and OsSIZ2.]]

To identify the subcellular localization of OsSIZ1 and OsSIZ2 in rice, we used GFP and RFP reporter gene fusion constructs in rice protoplasts. The GFP sequence was fused to the C-termini of the OsSIZ1 and OsSIZ2 cDNAs under the control of the CaMV35S promoter (Fig. 3a). Each chimeric OsSIZ1/-2::GFP construct was introduced into rice protoplasts along with an nuclear localization signal (NLS)::RFP construct that represented an authentic nuclear localization marker (Heikal et al. 2000). To further test and monitor potential monocot/dicot differences in localization masked by the heterologous system used, we expressed the GFP fusion constructs in tobacco epidermis cells and in Arabidopsis protoplasts (data not shown). Taken together, the subcellular distributions of each OsSIZ1/-2::GFP signal clearly overlapped with those of the NLS::RFP red fluorescence signals, indicating that OsSIZ1/2 are localized to the nucleus (Fig. 4b).

===Evolution===
[[File:NPH 3538 sm FigS1-3.jpg|right|thumb|300px|'''Figure 3.''' Schematic diagram of SIZ protein domains, amino acid comparison of SIZ1 protein with its orthologs in other plant species. .]]

Similar to most of the characterized and annotated SIZ/PIAS (SAP and MIZ/Protein Inhibitor of Activated STAT) SUMO E3 ligases from all organisms, rice SIZ1 also contains SAP, PINIT, SP-RING, a SUMO binding motif (hhhSXSaaa), a C-terminal nuclear localization signal (NLS) and the plant-specific PHD domain (plant homeodomain with C4HC3-type Zn-finger) (Fig. 3a,b). PINIT (Pro-Ile-Asn-Ile-Thr) and SP-RING, containing a zinc-finger (C2HC3), are essential for SUMO E3 ligase activity, and SAP (scaffold attachment factors SAF-A/B, Acinus, PIAS, a helix–extended loop–helix) forms a helix–extended loop–helix structure probably involved in DNA binding (Aravind & Koonin, 2000). Amino acid comparison revealed that all plant SIZs possess all consensus domains (Fig. 3b). A BlastP search analysis with SIZ1 amino acid sequences used as a query was employed to generate a rooted phylogenetic tree to illustrate the relationship of rice SIZs to their plant orthologs. As shown in Fig. 3c, rice SIZ1 and SIZ2 are positioned in different clades, and AtSIZ1 is closer to rice SIZ1 than to SIZ2. Sequence similarity analysis also confirmed that rice SIZ1 is closely related to AtSIZ1 (Fig. 3d). The amino acid sequences of SIZ1 and SIZ2 showed high similarity to sorghum loci Sb09g002225 and Sb08g000380, with 65.0% and 57.1% identity, respectively (data not shown). This is probably because both rice and sorghum originate from the grass family and have high synteny for sharing similar panicle architecture.
You can also add sub-section(s) at will.

==Labs working on this gene==
1
Division of Applied Life Science (BK21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, 660-701, Korea

2
College of Natural Resources and Life Science, Dong-A University, Busan, 604-714, Korea

3
Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana, 47907-2054, USA

4
King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia

5
Departments of Plant Biology and of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

==References==
'''ref 1''' PARK, H. C., KIM, H., KOO, S. C., PARK, H. J., CHEONG, M. S., HONG, H., BAEK, D., CHUNG, W. S., KIM, D. H., BRESSAN, R. A., LEE, S. Y., BOHNERT, H. J. and YUN, D.-J. (2010), Functional characterization of the SIZ/PIAS-type SUMO E3 ligases, OsSIZ1 and OsSIZ2 in rice. Plant, Cell & Environment, 33: 1923–1934. doi: 10.1111/j.1365-3040.2010.02195.x

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0719100|
Description = DNA-binding SAP domain containing protein|
Version = NM_001057627.1 GI:115454982 GeneID:4333925|
Length = 8026 bp|
Definition = Oryza sativa Japonica Group Os03g0719100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:29888631..29896656|
CDS = 29888925..29888969,29890016..29890096,29890498..29890546,29890633..29890711,29890796..29890955 ,29891341..29891474,29891848..29891916,29892098..29892185,29892430..29892505 ,29893301..29893374,29893512..29893619,29894033..29894194,29894517..29894621 ,29894704..29894784,29894872..29895886,29896120..29896235|
GCID = <gbrowseImage1>
name=NC_008396:29888631..29896656
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:29888631..29896656
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcgctcgaccccgccgacgatccgctgctcgccgattgcaagtacaagttgaatcactttagaataaaagagctaaaggatgtcctgcatcagcttggacttccaaagcaaggaaggaaacaggaacttgtggacaaaattatagcagtattgtctgatcaacaagaacaagactccagattgaatggcttgccaaacaaaaagatggttgggaaagaaactgtggctaaaatagttgatgacacttttgcaaaaatgaatggctccacaaatgctgttccagcctctagaaatcagactgactcggggcacattgtaaagcctaaaaggaagtcagatgattctgctcagttggatgtcaaagtccgttgtccctgtggttattccatggccaatgattccatgattaagtgtgaaggtccacaatgcaatacacagcaacatgtaggctgcgttatcatatctgagaagcctgcagacagtgttcctccagaattaccaccacacttttattgtgatatgtgccgaatcactcgagctgaccctttctgggttactgttaatcatcctgtacttccagtctcaataactccctgtaaagtagcatctgatgggtcatatgctgtacagtatttcgagaagacctttccgctatcaagagctaattgggagatgcttcagaaagatgaatatgacctccaggtttggtgtatcctattcaatgatagcgtacctttcaggatgcagtggcctttacattctgatattcaaattaatggtattcctattagggttgtcaacaggcaacccacacaacagttaggggtgaatggtagagacgatggtccagttttaacagcatatgtgagagaagggtctaataagattgtcctatctagaagtgactctcgcacattctgtttgggagtcaggattgccaagaggagatctgtagaacaggtcctatctttggtgccaaaggaacaagatggtgagaactttgataatgcccttgctcgtgtgcgtcgctgtgttggtggtggaaccgaggcagataatgctgacagtgacagtgatattgaagttgtagctgattctgtctctgtgaatctccgatgccctatgactggttcaaggatcaagatagctggccggttcaaaccctgcgttcacatgggttgttttgatttagaagcttttgtggaacttaatcaacgttcaagaaagtggcagtgcccaatttgcctgaaaaactactctttggacaacataatcattgatccttacttcaaccgcataactgctttggtccaaagttgtggagatgatgtatctgaaattgatgtcaagcctgacggttcctggagagtcaagggtggagcagaactgaagggtcttgcacagtggcatttacctgatggcactctctgtatgcctacagatacaaggtccaagcccaacataagaattgtaaagcaagagattaaagaagaaccgttgtctgaagaaacaggtggccgtcttaagttgggaatcagaagaaacaacaatggccagtgggaaattaacaagagattggattctaacaatggtcagaatggatatattgaagatgaaaactgtgttgtttcagcaagcaacactgatgatgagaacagcaaaaatggaatctataatccagaaccagggcaattcgatcaactaaccagcaatatctatgatcttgattcttctcctatggatgcacattttcctccagcaccaacagagcaggatgtaatagttctgagtgactcagatgatgataatgttatggtgttatcacctggtgatgttaatttcagttcagcacatgacaatgggaatgcattcccacccaacccacctgaagcttcaggaatttgtggggaacaacctagaggagccggcccagatgtgacttcatttcttgacggtttcgatgatctggaactgccattttgggaatcttcgagctctcaagatgctgcaggcacacaggtgacagacaatcaatgcgaaatgcaaaatttcattgtcaaccatcaatttttgcacgagccaattttaggggttaacttgggtggaacagcagcatcaaacacactggaatgtgagcatgatggtgcgttacaagcttgtcagtcgagtgatcaagatggtgatcagaatcaaacatgccatgatgggcactccggggatttgacaaatcttagtatcattagcactcaagatagtttaactaatggcaaaaatgcttctcagaaaaggacaaactgtgaagatggcacggcaggtttagacggctcagttgtcaggagcgcgaatggcttgagaggagagatgccaccacttgggcaggagcaggatcgtacagttagacaaaagttgatattaacaatcgagtcagactctgattag</cdnaseq>|
AA = <aaseq>MALDPADDPLLADCKYKLNHFRIKELKDVLHQLGLPKQGRKQEL VDKIIAVLSDQQEQDSRLNGLPNKKMVGKETVAKIVDDTFAKMNGSTNAVPASRNQTD SGHIVKPKRKSDDSAQLDVKVRCPCGYSMANDSMIKCEGPQCNTQQHVGCVIISEKPA DSVPPELPPHFYCDMCRITRADPFWVTVNHPVLPVSITPCKVASDGSYAVQYFEKTFP LSRANWEMLQKDEYDLQVWCILFNDSVPFRMQWPLHSDIQINGIPIRVVNRQPTQQLG VNGRDDGPVLTAYVREGSNKIVLSRSDSRTFCLGVRIAKRRSVEQVLSLVPKEQDGEN FDNALARVRRCVGGGTEADNADSDSDIEVVADSVSVNLRCPMTGSRIKIAGRFKPCVH MGCFDLEAFVELNQRSRKWQCPICLKNYSLDNIIIDPYFNRITALVQSCGDDVSEIDV KPDGSWRVKGGAELKGLAQWHLPDGTLCMPTDTRSKPNIRIVKQEIKEEPLSEETGGR LKLGIRRNNNGQWEINKRLDSNNGQNGYIEDENCVVSASNTDDENSKNGIYNPEPGQF DQLTSNIYDLDSSPMDAHFPPAPTEQDVIVLSDSDDDNVMVLSPGDVNFSSAHDNGNA FPPNPPEASGICGEQPRGAGPDVTSFLDGFDDLELPFWESSSSQDAAGTQVTDNQCEM QNFIVNHQFLHEPILGVNLGGTAASNTLECEHDGALQACQSSDQDGDQNQTCHDGHSG DLTNLSIISTQDSLTNGKNASQKRTNCEDGTAGLDGSVVRSANGLRGEMPPLGQEQDR TVRQKLILTIESDSD</aaseq>|
DNA = <dnaseqindica>295..339#1386..1466#1868..1916#2003..2081#2166..2325#2711..2844#3218..3286#3468..3555#3800..3875#4671..4744#4882..4989#5403..5564#5887..5991#6074..6154#6242..7256#7490..7605#actccagccgcccccctggctggctctgcctctggctccggttcttgccgctcgcgatccccaaagcgcggcaatccgccgcacgcgcgcgcgcgcgctcgcactccctccgccccgccgcgccgtccctcctgacgcggcgtctcgccgccgccgccgccgccgccaggttggtggtcgtcgcttcaggttcgcggaagccagcgcgtgggcaagcgtcctggcctccagggacgagccagggttcgccgggaaggaggacggtgagttcattccccatcggattttccagccatggcgctcgaccccgccgacgatccgctgctcgccgattgcaaggtaaccgtaatccatctaatcttttttgagtaaatttcacaaaactacaggtattttgcataatttatcacgaaactacaaatttaagagcttgtttcacaaacctacagattcagtgtctccgtttatcacaaagctacaggtattttgtacaatctattacaaaactacagatttaagaacttgtttcacaaaactatagatttagtgtattcgtttatcacagagctacacatttagtgtctttatttatcacaaaactacatatttagtgtctccattatcacaaaactacattttttaataatgctaaaacctgtagatttgtgataatgaagatactaaacttgtagttttgtgataaatgaagatattaaatctgtagttttgtaaaacaagttcctaaatctgtagttttgtgatatattgtgcaaagtacctgcagttttgtgataaacgaagacattgaatctatagttttgtgaaacaagttcttaaatctgtagttttgtgatagatcgtgcaaagtatatgtagttttatgaaatttactcaccttttttttttggggtggggggggggggggttgcggttggtctggatggatggtgccatgtctcttgcttctacctaaagccctcctcttccatccgtcccgattcattaagattttccaactcgatctgcgtataccgctcctttctttacaattcatcagtttcatgttgccttagattacccccatttttcatgttaccactatctttcttttatcaagaacccatcttttttgcatttccggctggaatgcaaggcactggggcagccacttgacagcatgcttaatttcttgttcaaaaggttgctcacaaatctattgtcaatgctacattaatcttttttcacttgtgctgtttcccatagcttggcaactttgtcttactaacgttgctatatggaacacatttggtggatggattatttatgttggtgagcccaaatagcttatgcctcctttttatgtttaaacatcattttgcatgcagtacaagttgaatcactttagaataaaagagctaaaggatgtcctgcatcagcttggacttccaaagcaaggaaggaaacaggtttgttcactgcatcttccttcatctttttcattacacaattactttctagtcatcgcttaattaacaccaatggcgctagttaccattcttccttttccttttcttaactacgtaaaatcgctccctctcttaaaagaacttcctaaaaaaactggtccacccttaaaaagacatcgtctaaaaaaaaatctggtgatcctgtaatcccgttaccatgtatggacatgtaactagtattgtgggaaaatgtgagattcagttgggtttaatggcctccatggcaatagcccagacatattttgcaattaaattaacttgtatgtgacttctagtattgcacatatgggatttgttcacttatggaccatttgggtcttgtgctttcgcttcatttacaggaacttgtggacaaaattatagcagtattgtctgatcaacaagaacaaggtacattacactgttgcttttgaaattttgatattttttactggtgtcgactactaaaaggtcatgatttcattttgttcttgcagactccagattgaatggcttgccaaacaaaaagatggttgggaaagaaactgtggctaaaatagttgatgacacttttgcgtgagcttactttctttatatacctttttcaatgatccaattagctatttacaaggcattataaactttgtgtactgcttccagaaaaatgaatggctccacaaatgctgttccagcctctagaaatcagactgactcggggcacattgtaaagcctaaaaggaagtcagatgattctgctcagttggatgtcaaagtccgttgtccctgtggttattccatggccaatgattccatgattaaggtactgttcagaaattcatattacagttacaagtataatcatgcaatatctaattgtcctccaccagccaatccaacagaacctgtaaaatttcttctaaaccttgtactgatcatgctgaattatttgggttactattcatttatgatttatccatcatgagtcttatgtgctgaaacttgaatacagctaattgttttgggttacacatttattcatcgtgagtcttatgtaaaacagaaatgttataccaaatgtagcacctaagtgttgtgttatttcattatttcaaaaattggtagcatctcgtaatctttttcgtgacagttcctgttgcttgcttctgtttagtgttattatccacaatttgtttgccccttttcagtgtgaaggtccacaatgcaatacacagcaacatgtaggctgcgttatcatatctgagaagcctgcagacagtgttcctccagaattaccaccacacttttattgtgatatgtgccgaatcactcgagctgacccgtaagttcttctatactctctgatgtagcaactcatccttactgaaccacccaattgtcctgcgatcacatgcttaatttcttccttgtgccataatgatgtggtatatcaagttcttcatggtagttcaagctcaaacatcttattcttttagttctgtggcattatttatttatttatttatagaactattttttttctgagcaaaaccattttttctgtgaactttaaaactctatgatttctctttattcccgttatgtgcgttttcttgtacgacattgttaggctgagtacataatttaccaaattcctatttggtcttgatatcatagcatattgttctaacatgatttccttctttaacttgcagtttctgggttactgttaatcatcctgtacttccagtctcaataactccctgtaaagtagcatctgatgggtgagttatctattattttgtcaccaaagaacattatttaatgttggttcccaaaatgttggtattgtcacatgtggaatagaagggtcgatctgggggcaagtgggcaggatggatcctacattgttctccacttccttttttcccctcttttttgtttgtgattcatgcttatgtccaggtcatatgctgtacagtatttcgagaagacctttccgctatcaagagctaattgggagatgcttcagaaagatgaatatgacctccaggttaactccctgttccaatgaaccatcattaattttgttcacgatgaccattcctaatcattttgagccataacttattaagatctgtaaaatcgaacctttgcaaagacatctcagtatagagtataaactatggttattatgctttcttattgattttatgctagagaatatttgagttgtttgaccaatatgtatccaattatggccttgattttctgttgcattctttattttcatgcaggtttggtgtatcctattcaatgatagcgtacctttcaggatgcagtggcctttacattctgatattcaaattaatggtatatgcctgagttttctgtgcagcaatgcatattctataacagggttaatagaaaacggtttattcagttaaagaatcatgtcctcaaactcattgcaaatggtatatgcctgagcttactgtacgtaacaatgtatattctataatagggttcatagagaacggtttattcagttagagaatcacgtcctcaaactcattgcactagttttgtttgtataatccatgctagactaacagaacagtgtcgtggaacatacattgtttaatacgctgctttgttatcagcagttcttttacagcttgttgaatttttggacggtgtgttttctgccttgaacttggttgaagggcctcataatctcttggctaatcagtgtttgctgttaggatgtcttgagcttatgttttctgtggtacaattgagcctatttgcccaagctaggtggatgatggtgcctgttactgcattaaaaaccaaacttcatcttcagctcatattttgttgcattaccagttgttttgctcctcgttttactttattgtgggtgatttgtgcatgcacatctttttgcatctatgttttcatgcacttgcatattcctttatttcggcaatttagaaatgtagcaccttgcaagtttacatccttttagctatatctgatctcttgtcacttcatacgttatcttaaacatgccgtgataaatggtcatctgtaccgaatagtagttgaaatgttcaatcataaatctgaactgttcatgttggcaattggttttcttttcttcaggtattcctattagggttgtcaacaggcaacccacacaacagttaggggtgaatggtagagacgatggtccagttgtaagcatcttattctcataaagcatgctattctcttattcaattattcatctgagatattatataca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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057627.1 RefSeq:Os03g0719100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

File:PCE 2195 f7.gif

2014-06-26T12:33:59Z

潘文波:

File:PCE 2195 f6.gif

2014-06-26T12:33:32Z

潘文波:

File:45y644t.gif

2014-06-26T12:13:35Z

潘文波:

File:1234235.png

2014-06-26T12:12:50Z

潘文波:

Os05g0125000

2014-06-26T12:03:54Z

潘文波: /* Evolution */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Sumoylation is a post-translational regulatory process in diverse cellular processes in eukaryotes, involving conjugation/deconjugation of small ubiquitin-like modifier (SUMO) proteins to other proteins thus modifying their function. The PIAS [protein inhibitor of activated signal transducers and activators of transcription (STAT)] and SAP (scaffold attachment factor A/B/acinus/PIAS)/MIZ (SIZ) proteins exhibit SUMO E3-ligase activity that facilitates the conjugation of SUMO proteins to target substrates. Here, we report the isolation and molecular characterization of Oryza sativa SIZ1 (OsSIZ1) and SIZ2 (OsSIZ2), rice homologs of Arabidopsis SIZ1. The rice SIZ proteins are localized to the nucleus and showed sumoylation activities in a tobacco system. Our analysis showed increased amounts of SUMO conjugates associated with environmental stresses such as high and low temperature, NaCl and abscisic acid (ABA) in rice plants. The expression of OsSIZ1 and OsSIZ2 in siz1-2 Arabidopsis plants partially complemented the morphological mutant phenotype and enhanced levels of SUMO conjugates under heat shock conditions. In addition, ABA-hypersensitivity of siz1-2 seed germination was partially suppressed by OsSIZ1 and OsSIZ2. The results suggest that rice SIZ1 and SIZ2 are able to functionally complement Arabidopsis SIZ1 in the SUMO conjugation pathway. Their effects on the Arabidopsis mutant suggest a function for these genes related to stress responses and stress adaptation[1].

OsSIZ1 Regulates the Vegetative Growth and Reproductive Development in Rice.Two homologous genes from rice (Oryza sativa) were isolated and designated as OsSIZ1 and OsSIZ2 based on amino acid sequence homology to AtSIZ1 and their phylogenetic relationship. The function in the vegetative growth and reproductive development in rice was investigated using OsSIZ1 mutants containing a T-DNA insertion. The results showed that the mutant Ossiz1 exhibited the significant changes in several growth and developmental parameters, including primary root length, adventitious root number, plant height, leaf and panicle length, flower formation, and seed-setting rate compared with wild type. Taking together these results indicate that OsSIZ1 plays an important role in regulating growth and development in rice[2].

Rice SIZ1, a SUMO E3 ligase, controls spikelet fertility through regulation of anther dehiscence.Genetic results revealed the co-segregation of single T-DNA insertional recessive mutation with the observed phenotypes in siz1. In addition to showing reduced plant height, tiller number and seed set percentage, both the siz1 mutant and SIZ1-RNAi transgenic plants showed obvious defects in anther dehiscence, but not pollen viability. The anther indehiscence in siz1 was probably a result of defects in endothecium development before anthesis. Interestingly, rice orthologs of AtIRX and ZmMADS2, which are essential for endothecium development during anther dehiscence, were significantly down-regulated in siz1. Compared with the wild-type, the sumoylation profile of high-molecular-weight proteins in mature spikelets was reduced significantly in siz1 and the SIZ1-RNAi line with notably reduced SIZ1 expression. The nuclear localization signal located in the SIZ1 C-terminus was sufficient for its nuclear targeting in bombarded onion epidermis.The results suggest the functional role of SIZ1, a SUMO E3 ligase, in regulating rice anther dehiscence[3].

Heterologous expression of OsSIZ1, a rice SUMO E3 ligase, enhances broad abiotic stress tolerance in transgenic creeping bentgrass[4].

GO assignment(s): GO:0009901, GO:0009737, GO:0009651, GO:0009408, GO:0009409, GO:0016925

===Expression===
[[File:Untitled.png|right|thumb|300px|'''Figure 1.''' Nucleus-localized SIZ1 in bombarded onion epidermal cells. .]]

In rice, SIZ1 was universally present in all examined tissues and all developmental stages (data not shown). Similar to most of the characterized and annotated SIZ/PIAS (SAP and MIZ/Protein Inhibitor of Activated STAT) SUMO E3 ligases from all organisms.

In Arabidopsis, AtSIZ1 protein is primarily localized in the nucleus, and PHR1, a MYB transcriptional activator, is its direct target in response to phosphate deficiency .Therefore, the subcellular localization is important to reveal the potential functions of rice SIZs. Recently, Park et al. (2010) showed the nuclear localization of OsSIZ1 and OsSIZ2 in rice protoplasts. To verify whether NLS in the SIZ1 C-terminus is responsible for its nuclear targeting, the NLS-containing C-terminal SIZ1 was fused to the N-terminus of eGFP for particle bombardment-mediated transient assay in onion epidermis (Fig. 1). The mRFP-tagged Ethylene Response Factor 4 (P35S::ERF4-mRFP:T35S), a known transcription factor, was used as a positive nuclear localization marker. Cells expressing the SIZ1–CT–eGFP fusion construct showed co-localization of GFP signals with ERF4 specifically in the nuclei (Fig. 3b). Our subcellular localization study suggested that the NLS-containing C-terminus of SIZ1 was sufficient for its nuclear targeting.

===Evolution===
[[File:NPH 3538 sm FigS1-3.jpg|left|thumb|300px|'''Figure 2.''' Schematic diagram of SIZ protein domains, amino acid comparison of SIZ1 protein with its orthologs in other plant species. .]]

Similar to most of the characterized and annotated SIZ/PIAS (SAP and MIZ/Protein Inhibitor of Activated STAT) SUMO E3 ligases from all organisms, rice SIZ1 also contains SAP, PINIT, SP-RING, a SUMO binding motif (hhhSXSaaa), a C-terminal nuclear localization signal (NLS) and the plant-specific PHD domain (plant homeodomain with C4HC3-type Zn-finger) (Fig. 2a,b). PINIT (Pro-Ile-Asn-Ile-Thr) and SP-RING, containing a zinc-finger (C2HC3), are essential for SUMO E3 ligase activity, and SAP (scaffold attachment factors SAF-A/B, Acinus, PIAS, a helix–extended loop–helix) forms a helix–extended loop–helix structure probably involved in DNA binding (Aravind & Koonin, 2000). Amino acid comparison revealed that all plant SIZs possess all consensus domains (Fig. 2b). A BlastP search analysis with SIZ1 amino acid sequences used as a query was employed to generate a rooted phylogenetic tree to illustrate the relationship of rice SIZs to their plant orthologs. As shown in Fig. S2c, rice SIZ1 and SIZ2 are positioned in different clades, and AtSIZ1 is closer to rice SIZ1 than to SIZ2. Sequence similarity analysis also confirmed that rice SIZ1 is closely related to AtSIZ1 (Fig. 2d). The amino acid sequences of SIZ1 and SIZ2 showed high similarity to sorghum loci Sb09g002225 and Sb08g000380, with 65.0% and 57.1% identity, respectively (data not shown). This is probably because both rice and sorghum originate from the grass family and have high synteny for sharing similar panicle architecture.

==Labs working on this gene==
Please input related labs here.

==References==
[1]Hyeong Cheol Park, Hun Kim, Sung Cheol Koo, Hee Jin Park, Mi Sun Cheong, Hyewon Hong, Dongwon Baek, Woo Sik Chung, Doh Hoon Kim, Ray A. Bressan, Sang Yeol Lee, Hans J. Bohnert, Dae-Jin Yun.Plant, Cell & Environment, 2010, 33(11): 1923-1934

[2]Huadun Wang, Kousar Makeen, Yan Yan, Yue Cao, Shubin Sun, Guohua Xu.Plant Molecular Biology Reporter, 2011, 29(2): 411-417 DOI: 10.1007/s11105-010-0232-y

[3]Saminathan Thangasamy, Cian-Ling Guo, Ming-Hsiang Chuang, Ming-Hsing Lai, Jychian Chen, Guang-Yuh Jauh.New Phytologist, 2011, 189(3): 869-882 DOI: 10.1111/j.1469-8137.2010.03538.x

[4]Zhigang Li, Qian Hu, Man Zhou, Joshua Vandenbrink, Dayong Li, Nick Menchyk, Shane Reighard, Ayla Norris, Haibo Liu, Dongfa Sun, Hong Luo
Plant Biotechnology Journal, 2013, 11(4): 432-445 DOI: 10.1111/pbi.12030

==Structured Information==
{{JaponicaGene|
GeneName = Os05g0125000|
Description = DNA-binding SAP domain containing protein|
Version = NM_001061052.1 GI:115461834 GeneID:4337672|
Length = 11525 bp|
Definition = Oryza sativa Japonica Group Os05g0125000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|
AP = Chromosome 5:1404985..1416509|
CDS = 1405234..1405260,1405979..1406059,1406301..1406343,1406547..1406625,1406734..1406893 ,1407008..1407135,1407577..1407645,1408239..1408326,1408959..1409034 ,1409210..1409283,1411078..1411185,1412226..1412387,1412622..1412726 ,1412890..1412970,1413046..1414246,1415097..1415242|
GCID = <gbrowseImage1>
name=NC_008398:1404985..1416509
source=RiceChromosome05
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008398:1404985..1416509
source=RiceChromosome05
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggacctggtttccagctgcaaggataaactggcatactttagaataaaggaactcaaagatatattaaatcagctcggcttaccaaagcaaggaaagaagcaggatcttattgatagggtgttggcactcttaacagatgagcaaggtcaaaggcatcatggatggggaaggaaaaattctctcaccaaggaggcagtggcaaaaattgttgatgatacatacaggaaaatgcaaatccaatgtgctcctgatctagccaccaggagccacagcggatcagatttcagtttcaggcctatagaggaagcctatgactctttccagccagaggccaaagttcgctgcatttgcagtagcacaatggttaatgacagcatgatccagtgtgaagatcagcgatgccaagtgtggcaacatttgaattgtgtactcattccagataagcctggggagagcgctgaagttccacccgttttctactgtgaattatgccgactgagtcgggcagacccattttgggtcactgctggaaatccattactcccagtgaaattcgtgtcatctggtgttacaaatgatggaacaagtgtacctcaaagtgtggagaaaagcttccagctttctcgatcagatagagaaactgtccagagacaagaatatgacctccaggtttggtgcatgcttttgaatgacaaagttcagttcaggatgcagtggccccaatatgcagaattgcatgttaatggtatttctgtacgagtagtgactagacctggttctcaattactagggataaatggacgggatgatggtccactgataaccacatgcagtagagagggaattaataaaatttgcttatcaagggtcgatgctcggacattttgctttggtgttcgaattgctaaacggaggactgttgctcaggttttgaacttagttccaaaggaagctgaaggagagtcttttgagcatgctcttgctcgtgttcggcgctgtctcggaggtggagacactgcagagaatgctgatagtgacagtgatttggaagtggttgcggagtctgttacagtcaaccttcgttgccctaatagtggatccagaatgaggattgctgggagattcaagccttgcattcacatgggttgttttgatcttgaaactttcgtggaattgaatcaacggtcccgcaagtggcaatgtccaatatgtttaaagaattactctcttgagagcttgatgattgatccttacttcaataggattacttctctgttgcgcaattgcaatgaggatgtcaatgaggttgatgttaagcctgacggatcttggcgtgtgaagggtgatgctgcaagtagagaattatctcagtggcatatgcctgatggtaccctttgtaatcctaaggaagatgtcaaacctgccatgcaaaatggaaatgaacaaatgatggaaggtacttctgatggacagaaatctttgaaaattggaataaagagaaatccaaatggaatctgggaagttagtagcaaagcagatgacaagaagccttctgtggttggaaatcgcatgcaaaacaatagtgggttccgagctctaaacaacattatgcatatgagcaatagcccaactagtagttatagagacggggaagacccaagtgtgaaccaagagagcaataggcatgttgacttatcattgaacaatggtaataatgagtttgacagtttctctctcaattttggccaagcatgcaatacagatgatagaccacagcaacaacataatgccacagacgtcattgttcttagtgattctgatgaagagaatgatgctatggtttgtccaccagctgtctatgacaatactaccactgcaaatggtagtggttttcctttcaccactaatggtattggatatactgaaaggtaccaggaagatgccggcgttggtacaagtggccttggtttattgagtaacaatgttgatgattttgagatgaataactggcaaatgcattcttcttatcaacaacctgaacaaggcttccagttttttgggaatgatactgatgtccataatacttttgttggttcacacaattcctttggcttagcaccaaatgactattctcttgattgtaatgttggcgtagaggaggcttcggtaactcctgctctttcagtctgccggaatagtaatgaaatgcatggaagtttggttgataacccactggctttggttggcgatgatccatccttgcaaatttttcttccaagtcaaccttcctctgttcctcttcaggaagaacttagcgagcgtgctaatgcaccaaatggggttcagtctgatgattggatatcccttacactcgcagcgggtggaggtggtaacgaagagcctgcacctgctgatgtcaattcacagccacaaattccatcaacagagacagggatcgaaccattgaccgatgctgcttctgcatttctgagcacaaacattgaaagacgtagcggagctgatttaaatccaagaaggatagaaaatatattttctcatcctcgccagcctcggtctgttaggcctcgactctgtttatcaatagatactgattctgagtag</cdnaseq>|
AA = <aaseq>MADLVSSCKDKLAYFRIKELKDILNQLGLPKQGKKQDLIDRVLA LLTDEQGQRHHGWGRKNSLTKEAVAKIVDDTYRKMQIQCAPDLATRSHSGSDFSFRPI EEAYDSFQPEAKVRCICSSTMVNDSMIQCEDQRCQVWQHLNCVLIPDKPGESAEVPPV FYCELCRLSRADPFWVTAGNPLLPVKFVSSGVTNDGTSVPQSVEKSFQLSRSDRETVQ RQEYDLQVWCMLLNDKVQFRMQWPQYAELHVNGISVRVVTRPGSQLLGINGRDDGPLI TTCSREGINKICLSRVDARTFCFGVRIAKRRTVAQVLNLVPKEAEGESFEHALARVRR CLGGGDTAENADSDSDLEVVAESVTVNLRCPNSGSRMRIAGRFKPCIHMGCFDLETFV ELNQRSRKWQCPICLKNYSLESLMIDPYFNRITSLLRNCNEDVNEVDVKPDGSWRVKG DAASRELSQWHMPDGTLCNPKEDVKPAMQNGNEQMMEGTSDGQKSLKIGIKRNPNGIW EVSSKADDKKPSVVGNRMQNNSGFRALNNIMHMSNSPTSSYRDGEDPSVNQESNRHVD LSLNNGNNEFDSFSLNFGQACNTDDRPQQQHNATDVIVLSDSDEENDAMVCPPAVYDN TTTANGSGFPFTTNGIGYTERYQEDAGVGTSGLGLLSNNVDDFEMNNWQMHSSYQQPE QGFQFFGNDTDVHNTFVGSHNSFGLAPNDYSLDCNVGVEEASVTPALSVCRNSNEMHG SLVDNPLALVGDDPSLQIFLPSQPSSVPLQEELSERANAPNGVQSDDWISLTLAAGGG GNEEPAPADVNSQPQIPSTETGIEPLTDAASAFLSTNIERRSGADLNPRRIENIFSHP RQPRSVRPRLCLSIDTDSE</aaseq>|
DNA = <dnaseqindica>250..276#995..1075#1317..1359#1563..1641#1750..1909#2024..2151#2593..2661#3255..3342#3975..4050#4226..4299#6094..6201#7242..7403#7638..7742#7906..7986#8062..9262#10113..10258#aaaccacaacgaactaccccctcctcgtcgagccgacgcgagagaggaaagtgggttgcggcttgctgcgcgtgtggagtcgccattccccaattcgctgcgccgcccgccggatctcgtcttgccccctgcggcggcggtttgggcccccccttccgatcggtttcccccccgcacatggtcgtggcggcggcggaggtggtggtggtgcgggagtagggaggcgggcgaaccgaggcggcggcgccgatggcggacctggtttccagctgcaaggtagactcgcgttcccccgcccgccggtaaattcgaccccgatctccgccgattccgcggcgtaattcggcgccggggggggggaggggattcgttgcgtcgcgcgcgtctgttttggggcggggaaggttgcggcgtgcctggccccgcccttgctcgccgtgtttgcctgtagatgatgagatgattagttagttttgtcgatagttttagttttttttttccagtggttgctgtggagtttattagtgcctagctctctggcgtttagggttgcggattaatcggtgatttcagtatagcacctggcgaggggcttcactgggcagttcaattgcttaggctggcgctttcgcttgttggcaaatcccccacatttgcttgatccgtagtttgtcccatgctgtatggttttgtatcatacgggaagcatccagatgtttcatgtcatactaatcactaaataagttgctcagaaaattgctaattttcactttcttcagtttggtagtttgggtccttgtgttccatgacagttaatttagcatggttagttgcgtcatgcttaattccaggcaagagtatctctgctttgatttggaattgatcccagtatttagtccatggatcaaacgccagttgtaccttatgtatgtgtcatgccaaatgaattgctttggtaactttagcattgacccctttgcctttttcattgcaggataaactggcatactttagaataaaggaactcaaagatatattaaatcagctcggcttaccaaagcaaggaaagaagcaggtcagcaaattttgttcctgttaatcttttttacatcaccatctatcagacggcagtttcaactaaatgagaatatgagatgtaagcttttgaggtgaaccggtagtatagactctcataatacagcattggtgggatctttggtggtaatggtggtctgatatgaagagctcatttcaatgagctatttcttagctcgttgtcttttgttttctgatatacttctcttatattctcacaggatcttattgatagggtgttggcactcttaacagatgagcaaggtatgcatttgtctgttgtttctgcttccctggtctatgttagtaccttacatatcaagtcatgtactgacatgctaaaatttgcactgtttcattccctgtatttgtttcagtggtgtctaatccatcagcatgaacggatgatgccttttctattttctagtcttaaaaacctaaatttgtgtgttcttttcttgttgcaggtcaaaggcatcatggatggggaaggaaaaattctctcaccaaggaggcagtggcaaaaattgttgatgatacatacaggttaaatttcattgtttcataaaattgaccatgtggcataatagtgtggatatttgagatgaaatcagtggtgcttctcttttgagatctttatgccttcattagcaggaaaatgcaaatccaatgtgctcctgatctagccaccaggagccacagcggatcagatttcagtttcaggcctatagaggaagcctatgactctttccagccagaggccaaagttcgctgcatttgcagtagcacaatggttaatgacagcatgatccaggtattataacattttttatttcttacattcctttctttttgaaagatcgttacggcatgtgccatccctctttttattattattatttaaatcatgtatcatgtctttctgcagtgtgaagatcagcgatgccaagtgtggcaacatttgaattgtgtactcattccagataagcctggggagagcgctgaagttccacccgttttctactgtgaattatgccgactgagtcgggcagacccgtatgtctttctactatttgatttttctttgggtggggttaagcaggtaaatctgtggtggaaaccactgcattttttaaagaaaaatcaaaacaaaaacatatttagggaaggtgatagtacatgctcttgtaattccatgtgcaaacttgattttgcttgtgagatatgaaaatggaaaacagtgaacgaaaagacaaacatcaagaattcaagatgtgtggcttttaccttgttgtttctcaatttcatatctcattaatgaaattagttttgatcttctgatttcacacagtttaataatgtatcacctcctctgtgcatgagattacttggggattttttgaaacttgtacaaaatggtttccagtttatcaccatatattctacaaaatgggattaagttttgatttgctgcagctacatgctttgaatttgcagattttgggtcactgctggaaatccattactcccagtgaaattcgtgtcatctggtgttacaaatgatgggtgggtataaatggattcttcttactggcaacattttaactgataataatccgtggctgactatttatttattattgatgctcttctttctctatttcgtctttgcaaaaatggcagtttttccaacacctttatttacattcactggtagctcgctaggctctccctttcagtcactccttcaaatagaaaaacagttcatgacacttttttcatcatgcctcttaaatttttttcattgttgcacaatcatagaatgtttacagttacactacggctcataattacatacgtaggtaaaatccattgttgatacttgatagtcatgagtttactttgtcacatgcctctccatatattgtacaaggctcttcttagtagttacttgtcacattataataatccttgtaacatatggtaaattctccagttaacgttatctgtgactatacaacattttgtctagcacatttatggacaacagtatgcttagaaggttcaattgctattctatttactctaaattgtctcgtttaatgatcatgtgttacttgtgatgtatgacatccttattaatcctctcatatctttagaacaagtgtacctcaaagtgtggagaaaagcttccagctttctcgatcagatagagaaactgtccagagacaagaatatgacctccaggtttgttctattctacaaagctaagttaaatatgcttgagttttggagtcctcagttcaacttaatattgcgtcaatattgtaatatcataatgctcttgagtaccaagtaagaaaccatatgggtttatgccactcagttgtttaggacgttcataagtttttttgttgattttaggcttatatgaatagctcttatcaatggtgaaggcaaacctttgaccaattcattttccttttgtaacatggaattcacacaatatgagtaatgcgtctattgtgcaattgttttcattggttgtattgtgaagacaaagcactgtggataagttatgttctctatgtaacatgaaatattcttaatttgttctgttacaagccaactgtatttactttgtgttgttgcaaattttcatctgtaccatgtggatgctacttgctagcattgtacaaagaaattttctttgtgcaaaagtttgaatttatgtggaccaggctgatttcgaattcattttgtccaatgatgaaacatggtttaattcatttcgtattgtttttttccttgcataaatctaagatatgcatgcatatattgtttgtttatactatctgaagcagtgaatgtgttgacaggtttggtgcatgcttttgaatgacaaagttcagttcaggatgcagtggccccaatatgcagaattgcatgttaatggtattgttgatgattttgtagcacaatttgattcactttcatatttgaattgcatcccagcttaaatattgtcttatatgttgtgcagttatgctgttgttggttaatacttcacgttggtctttctttaaaataagttactatctgtgtaacattagtttcattttatgttcaggtatttctgtacgagtagtgactagacctggttctcaattactagggataaatggacgggatgatggtccactggtaagttcaatttccttctgtcacattggtaagatctagatactaattggcagagatttggctatcctatttgatattcctgtatgttattctattttaagttgtgccatttttgttgttctccggttgacagaacctaacgttattgagtggagttacaaccttaaaatggaaccatgttatgttacaaaacctttgggcttgttttcattgatctgtagttaaaatttgctttatcttctagtggcggcctgtaaaaatttgcttctgtatttttaatgctcctatgaaatctcctaaaatcatattcgcagtaactcttaggtggaaagtctcattgagaatcttagctggatgtgtttagtatgagaaaaatttactgcccagtttgaaacagattattgactagattattgtcatggattatttacatgatcatagatatagattaaatatttgataaataaacttaacgaatacagcatagaacaagggatctaaataatgcagtagaagaaaggttttttttttttttgagaaaaacgtagttttagaggcatggagcaaataatctgtgacagtaatccagttaagtagctgaaaagaacagggcctggttgaagatccagttaagcttgcagagtcttgcagctaggggtgatacagttgaaaaccagcattaaggtttggaagaatagtgcattctcttagtttaggaagtactgtgagaacaaccatcggttgacagtagttgttgtcttatctagtcactaggaatcatggccatgagtctgaatcatcagtcgccacaacagtatcacctatcttacccagtgggcaccagccaccatgtgaatcttgcatatggagagctggagacttggcttattgcttattctggtttctaatgattgtaaggctttctattcttattacgcatatcctgtctacgaaaaaggacaatatgccactccattcgttgggtttagacgaaatgccacccactagaatgcaaaggataaaatgccactcaataggttaccttcaggatattttgccactttggggggattttaagaccaaaatacccttggctgtggagagagagaggaagaaagggaaatggctctgctcgccggtggcagcggtgagcgtgcgggggtgagctgcgatgggggaggacagcggcgacgatggggggaggaggacggcagcgctgacgggacggcctcacgcgctcgcagcttgccacctccggcgcctcccccccccctccccccccccccccccccgcgcgtttgccactcaccgctcgctgctcacggcaccgccgtcctcctcccccatcgtcgtccgcccctgcacactcaccaccccccgtcggcaagcacggacactgcccagcttgaccttctccggcgctcccctttcccctctttcttcctctctccacagccaagggtatattggtcttaaaatccctcaaagtggcaaaatatcctgaaggtaacctattgagtggcattttatcctttgcattctagtgggtggcattttgtctgaacccaacgaatggagtggcatattgtcctccttctcctacttggcgttatgcttccattttaaatattcccaggcatagggcattatttttacatgaagcaagctttcacatgcattctttatatagtaacgcttgttgccacattgctttacttttaattatatttccaaagttttggttctgcatagcttgtactatcattctcatggttgttttccctttatttgcagataaccacatgcagtagagagggaattaataaaatttgcttatcaagggtcgatgctcggacattttgctttggtgttcgaattgctaaacggaggactgttgctcaggtatttccatttgaccttttgatttatgaaatgataattttcagattcacttctcccagatgtgtttgttgatttgattgaaatataatctccttttgaaatgtgtacaagcaaaactgaggtttctacatgagtttattgtattcttgaaccatattcctctgtatacatcattgcacttttaattatttcacaagaacgacatttatgagccacaagatcaaattaggaatttatactgtcgagatctttccaaacggttctcaaatttcactcttctctaagggtgctgtcaacactcatctgaccaggaaagaaatccagttagccgcttggtccatcaaccttcattgacattatggtcaagctctgagtactgatagggaaaactgcaaattacaccacaaaaaaaatcatatagtttgcaaagattaacattagctttatctgttggttggtggcaatgactaatagggatatttttgcaagttatgacttcaatgatctgagcattatacgcgtgtttctttttgaactgttgtttcttgtacaccaaactgcttttagtttagaactgttgaatggctttccagttcaatccattttgatggtgatgttcttaatagaaattcgttccacaaattcgctattgaagcatataaatacatatcttgcctaattgttgctgtgaagtgcttactcctctgttttcaaaagaaacaattttgctctacttttaagcagtatgtacattatggtcgtattatgttacatgttcctattgaagtagtcatgcatggtttagtttaacattttctgatgaattacaaaatttaataaagttattacctggaaatgtctgaaattttattaccctcctctttgcttaaaggaagaaacgtatattttaagcataataatgaaagggggaatgctaagcgctgtctttatttccttgagatgtttcattcctctagaaaggccaacaactctcttagctaacaccttttctgattctaattgagctattgtttaaccaggttttgaacttagttccaaaggaagctgaaggagagtcttttgagcatgctcttgctcgtgttcggcgctgtctcggaggtggagacactgcagagaatgctgatagtgacagtgatttggaagtggttgcggagtctgttacagtcaaccttcgttgccctgtgagtttttgttacgtataactgtttagttagcttaacatatgtttgttaagtgtacactacagtatgcagatatagtcattctatcaggttattgatgtccattgttcattgatctggagtagttttagcacatataggttctaatgtcatgtcaagatgtgtgtaaggctaagcctttcttgtgatgcaaaattggagactaatctaatttgttttctaacatgtcttcagaatagtggatccagaatgaggattgctgggagattcaagccttgcattcacatgggttgttttgatcttgaaactttcgtggaattgaatcaacggtcccgcaaggttagatcttgtgttccctgatgctcatccttcagatacatgtgtgttcatccaggaaagagttacttttttctggagctcacaacccaccttttgacatgttgatgctttcatattttccttttatatcctctgattgctcttttctcttgtttctatacagtggcaatgtccaatatgtttaaagaattactctcttgagagcttgatgattgatccttacttcaataggattacttctctggtaagttttcattgtacctctaaatctttttgtcagatccacagcattttgtgattatttttcttttggatgcagttgcgcaattgcaatgaggatgtcaatgaggttgatgttaagcctgacggatcttggcgtgtgaagggtgatgctgcaagtagagaattatctcagtggcatatgcctgatggtaccctttgtaatcctaaggaagatgtcaaacctgccatgcaaaatggaaatgaacaaatgatggaaggtacttctgatggacagaaatctttgaaaattggaataaagagaaatccaaatggaatctgggaagttagtagcaaagcagatgacaagaagccttctgtggttggaaatcgcatgcaaaacaatagtgggttccgagctctaaacaacattatgcatatgagcaatagcccaactagtagttatagagacggggaagacccaagtgtgaaccaagagagcaataggcatgttgacttatcattgaacaatggtaataatgagtttgacagtttctctctcaattttggccaagcatgcaatacagatgatagaccacagcaacaacataatgccacagacgtcattgttcttagtgattctgatgaagagaatgatgctatggtttgtccaccagctgtctatgacaatactaccactgcaaatggtagtggttttcctttcaccactaatggtattggatatactgaaaggtaccaggaagatgccggcgttggtacaagtggccttggtttattgagtaacaatgttgatgattttgagatgaataactggcaaatgcattcttcttatcaacaacctgaacaaggcttccagttttttgggaatgatactgatgtccataatacttttgttggttcacacaattcctttggcttagcaccaaatgactattctcttgattgtaatgttggcgtagaggaggcttcggtaactcctgctctttcagtctgccggaatagtaatgaaatgcatggaagtttggttgataacccactggctttggttggcgatgatccatccttgcaaatttttcttccaagtcaaccttcctctgttcctcttcaggaagaacttagcgagcgtgctaatgcaccaaatggggttcagtctgatgattggatatcccttacactcgcagcgggtggaggtggtaacgaagagcctgcacctgctgatgtcaattcacagccacaaattccatcaacagagacagggatcgaaccattgaccgatgctggtttgtctcccttttccgagtttagttctttattcatgtgacagttatattctaaggcaatggtttactcttgaaatatttttaccaacaacttaaaatttctatccatttgcttagtcatattttttttcagttgaattatttccttgtatgatattatatccgtgcgtgatactttgcttttccatgttttcataactagttaaattggctaacaattatcttaacataattttgttgtgaactgattactgactggaaagaaaacagtgatgaattagcacaatgtggataagactactggaaacatagttgctgctaacctttggtgatccttgagtatctcttcaactaagttaccaagcatgattattccacctttttgtttcatgagtaaagagttcattagtttttgctcttaatgcattttttaggggctgcagagacccacatcaccaattgtattgctaagcttatacttgtagcaaatgtcgattcacaccgggcctgctttctcttttctttagtaacactaccaattttgaaggaatttgcgtagtatcaagatatttattataccaaatgcttctaagcctgtcttctattatcagcccatctctagggtggtttggtagttacaatttctatggattttgctgtctgttatggtcgcctgtataacaggggttatgcttggttggaacatatgcaggaacatccaggtcttttgtctgctagtggttattgttgggtgttcccaccactatcatcatgtattatcagcaattattgttgtgattgctcagttggtccaaaatattggcataattcctttactcgttttttgcagcttctgcatttctgagcacaaacattgaaagacgtagcggagctgatttaaatccaagaaggatagaaaatatattttctcatcctcgccagcctcggtctgttaggcctcgactctgtttatcaatagatactgattctgagtagtttggacatcataacggggtaactgagtttgcattagtttggcaaagctgccatccagaaatcatgatttatactgaggtggggttatcggtcgtctggttgatgtaaagaaaaacaccagcattgatgctttgttgcctcaatggtttacaagctttcaagtgttctatttgatccaggagggatctgcatagaggacatctgatggtgagcataactgattagtccttttcccctgtgttactgttccagtttccttgtagtttgttgtttgcatgtgattaaagttgttaatttgcaagtgggcatgtcttttaccaaatgaaaagggaaagaatgacatagaagatcccttcccattcattaatgacaccatctggagttcgatcacaataggataaaaatgttgcagattataacaggataaataagagcaaattaatatgctgttagtttctaagcacatcatgaagttgaatattcattcttataaagcttgtttgtgcttcgattggctttagagcttacacatcatgttaggttctcttatttgcatttcccaaaatttagttgttgtctgacatatattcatatgtaacacaaaacatctacagtagtcgagattttcagataagatgggagtcgcagtgagtaagcatagctgtgtaggcttcttgttagaagcttgtctaaagtagttctttgccagcagtcaatgaataaaatttgcaatatatatgtctgcttctctgggtagtagaattattcgctgaagtagtccaaataagaagtaaaagatttgaacctcgtataacaagaatgttcccagtttcttgtgcagttgttaacagatgatgctataaggtttttactttaatatcacagaaattgttgaggcttgtcaacaaaaaaaggcatgatactgtagttacattccttctcattggaggtcgctaatgttttttttttttttctgttaggttggtatagaaaattttctaactgggtatcgaggcttaatgtggcgactggagcagtttgtacattttttttgttttgacttttactggatataaggaatagaggtggggtcatccctggaccctggatgcaagacaaatacatgtgaatgttttgggtgtaccatcattgtatttagggtttgggggtactaatttagttgtttattaacctggtatttgatggagaataaattattcctggaagtggcggccaataaacagagtcgttggggttttacttgggt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001061052.1 RefSeq:Os05g0125000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 5]]
[[Category:Chromosome 5]]

File:NPH 3538 sm FigS1-3.jpg

2014-06-26T11:59:47Z

潘文波:

Os05g0125000

2014-06-26T11:56:54Z

潘文波: /* Expression */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Sumoylation is a post-translational regulatory process in diverse cellular processes in eukaryotes, involving conjugation/deconjugation of small ubiquitin-like modifier (SUMO) proteins to other proteins thus modifying their function. The PIAS [protein inhibitor of activated signal transducers and activators of transcription (STAT)] and SAP (scaffold attachment factor A/B/acinus/PIAS)/MIZ (SIZ) proteins exhibit SUMO E3-ligase activity that facilitates the conjugation of SUMO proteins to target substrates. Here, we report the isolation and molecular characterization of Oryza sativa SIZ1 (OsSIZ1) and SIZ2 (OsSIZ2), rice homologs of Arabidopsis SIZ1. The rice SIZ proteins are localized to the nucleus and showed sumoylation activities in a tobacco system. Our analysis showed increased amounts of SUMO conjugates associated with environmental stresses such as high and low temperature, NaCl and abscisic acid (ABA) in rice plants. The expression of OsSIZ1 and OsSIZ2 in siz1-2 Arabidopsis plants partially complemented the morphological mutant phenotype and enhanced levels of SUMO conjugates under heat shock conditions. In addition, ABA-hypersensitivity of siz1-2 seed germination was partially suppressed by OsSIZ1 and OsSIZ2. The results suggest that rice SIZ1 and SIZ2 are able to functionally complement Arabidopsis SIZ1 in the SUMO conjugation pathway. Their effects on the Arabidopsis mutant suggest a function for these genes related to stress responses and stress adaptation[1].

OsSIZ1 Regulates the Vegetative Growth and Reproductive Development in Rice.Two homologous genes from rice (Oryza sativa) were isolated and designated as OsSIZ1 and OsSIZ2 based on amino acid sequence homology to AtSIZ1 and their phylogenetic relationship. The function in the vegetative growth and reproductive development in rice was investigated using OsSIZ1 mutants containing a T-DNA insertion. The results showed that the mutant Ossiz1 exhibited the significant changes in several growth and developmental parameters, including primary root length, adventitious root number, plant height, leaf and panicle length, flower formation, and seed-setting rate compared with wild type. Taking together these results indicate that OsSIZ1 plays an important role in regulating growth and development in rice[2].

Rice SIZ1, a SUMO E3 ligase, controls spikelet fertility through regulation of anther dehiscence.Genetic results revealed the co-segregation of single T-DNA insertional recessive mutation with the observed phenotypes in siz1. In addition to showing reduced plant height, tiller number and seed set percentage, both the siz1 mutant and SIZ1-RNAi transgenic plants showed obvious defects in anther dehiscence, but not pollen viability. The anther indehiscence in siz1 was probably a result of defects in endothecium development before anthesis. Interestingly, rice orthologs of AtIRX and ZmMADS2, which are essential for endothecium development during anther dehiscence, were significantly down-regulated in siz1. Compared with the wild-type, the sumoylation profile of high-molecular-weight proteins in mature spikelets was reduced significantly in siz1 and the SIZ1-RNAi line with notably reduced SIZ1 expression. The nuclear localization signal located in the SIZ1 C-terminus was sufficient for its nuclear targeting in bombarded onion epidermis.The results suggest the functional role of SIZ1, a SUMO E3 ligase, in regulating rice anther dehiscence[3].

Heterologous expression of OsSIZ1, a rice SUMO E3 ligase, enhances broad abiotic stress tolerance in transgenic creeping bentgrass[4].

GO assignment(s): GO:0009901, GO:0009737, GO:0009651, GO:0009408, GO:0009409, GO:0016925

===Expression===
[[File:Untitled.png|right|thumb|300px|'''Figure 1.''' Nucleus-localized SIZ1 in bombarded onion epidermal cells. .]]

In rice, SIZ1 was universally present in all examined tissues and all developmental stages (data not shown). Similar to most of the characterized and annotated SIZ/PIAS (SAP and MIZ/Protein Inhibitor of Activated STAT) SUMO E3 ligases from all organisms.

In Arabidopsis, AtSIZ1 protein is primarily localized in the nucleus, and PHR1, a MYB transcriptional activator, is its direct target in response to phosphate deficiency .Therefore, the subcellular localization is important to reveal the potential functions of rice SIZs. Recently, Park et al. (2010) showed the nuclear localization of OsSIZ1 and OsSIZ2 in rice protoplasts. To verify whether NLS in the SIZ1 C-terminus is responsible for its nuclear targeting, the NLS-containing C-terminal SIZ1 was fused to the N-terminus of eGFP for particle bombardment-mediated transient assay in onion epidermis (Fig. 1). The mRFP-tagged Ethylene Response Factor 4 (P35S::ERF4-mRFP:T35S), a known transcription factor, was used as a positive nuclear localization marker. Cells expressing the SIZ1–CT–eGFP fusion construct showed co-localization of GFP signals with ERF4 specifically in the nuclei (Fig. 3b). Our subcellular localization study suggested that the NLS-containing C-terminus of SIZ1 was sufficient for its nuclear targeting.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
[1]Hyeong Cheol Park, Hun Kim, Sung Cheol Koo, Hee Jin Park, Mi Sun Cheong, Hyewon Hong, Dongwon Baek, Woo Sik Chung, Doh Hoon Kim, Ray A. Bressan, Sang Yeol Lee, Hans J. Bohnert, Dae-Jin Yun.Plant, Cell & Environment, 2010, 33(11): 1923-1934

[2]Huadun Wang, Kousar Makeen, Yan Yan, Yue Cao, Shubin Sun, Guohua Xu.Plant Molecular Biology Reporter, 2011, 29(2): 411-417 DOI: 10.1007/s11105-010-0232-y

[3]Saminathan Thangasamy, Cian-Ling Guo, Ming-Hsiang Chuang, Ming-Hsing Lai, Jychian Chen, Guang-Yuh Jauh.New Phytologist, 2011, 189(3): 869-882 DOI: 10.1111/j.1469-8137.2010.03538.x

[4]Zhigang Li, Qian Hu, Man Zhou, Joshua Vandenbrink, Dayong Li, Nick Menchyk, Shane Reighard, Ayla Norris, Haibo Liu, Dongfa Sun, Hong Luo
Plant Biotechnology Journal, 2013, 11(4): 432-445 DOI: 10.1111/pbi.12030

==Structured Information==
{{JaponicaGene|
GeneName = Os05g0125000|
Description = DNA-binding SAP domain containing protein|
Version = NM_001061052.1 GI:115461834 GeneID:4337672|
Length = 11525 bp|
Definition = Oryza sativa Japonica Group Os05g0125000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|
AP = Chromosome 5:1404985..1416509|
CDS = 1405234..1405260,1405979..1406059,1406301..1406343,1406547..1406625,1406734..1406893 ,1407008..1407135,1407577..1407645,1408239..1408326,1408959..1409034 ,1409210..1409283,1411078..1411185,1412226..1412387,1412622..1412726 ,1412890..1412970,1413046..1414246,1415097..1415242|
GCID = <gbrowseImage1>
name=NC_008398:1404985..1416509
source=RiceChromosome05
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008398:1404985..1416509
source=RiceChromosome05
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggacctggtttccagctgcaaggataaactggcatactttagaataaaggaactcaaagatatattaaatcagctcggcttaccaaagcaaggaaagaagcaggatcttattgatagggtgttggcactcttaacagatgagcaaggtcaaaggcatcatggatggggaaggaaaaattctctcaccaaggaggcagtggcaaaaattgttgatgatacatacaggaaaatgcaaatccaatgtgctcctgatctagccaccaggagccacagcggatcagatttcagtttcaggcctatagaggaagcctatgactctttccagccagaggccaaagttcgctgcatttgcagtagcacaatggttaatgacagcatgatccagtgtgaagatcagcgatgccaagtgtggcaacatttgaattgtgtactcattccagataagcctggggagagcgctgaagttccacccgttttctactgtgaattatgccgactgagtcgggcagacccattttgggtcactgctggaaatccattactcccagtgaaattcgtgtcatctggtgttacaaatgatggaacaagtgtacctcaaagtgtggagaaaagcttccagctttctcgatcagatagagaaactgtccagagacaagaatatgacctccaggtttggtgcatgcttttgaatgacaaagttcagttcaggatgcagtggccccaatatgcagaattgcatgttaatggtatttctgtacgagtagtgactagacctggttctcaattactagggataaatggacgggatgatggtccactgataaccacatgcagtagagagggaattaataaaatttgcttatcaagggtcgatgctcggacattttgctttggtgttcgaattgctaaacggaggactgttgctcaggttttgaacttagttccaaaggaagctgaaggagagtcttttgagcatgctcttgctcgtgttcggcgctgtctcggaggtggagacactgcagagaatgctgatagtgacagtgatttggaagtggttgcggagtctgttacagtcaaccttcgttgccctaatagtggatccagaatgaggattgctgggagattcaagccttgcattcacatgggttgttttgatcttgaaactttcgtggaattgaatcaacggtcccgcaagtggcaatgtccaatatgtttaaagaattactctcttgagagcttgatgattgatccttacttcaataggattacttctctgttgcgcaattgcaatgaggatgtcaatgaggttgatgttaagcctgacggatcttggcgtgtgaagggtgatgctgcaagtagagaattatctcagtggcatatgcctgatggtaccctttgtaatcctaaggaagatgtcaaacctgccatgcaaaatggaaatgaacaaatgatggaaggtacttctgatggacagaaatctttgaaaattggaataaagagaaatccaaatggaatctgggaagttagtagcaaagcagatgacaagaagccttctgtggttggaaatcgcatgcaaaacaatagtgggttccgagctctaaacaacattatgcatatgagcaatagcccaactagtagttatagagacggggaagacccaagtgtgaaccaagagagcaataggcatgttgacttatcattgaacaatggtaataatgagtttgacagtttctctctcaattttggccaagcatgcaatacagatgatagaccacagcaacaacataatgccacagacgtcattgttcttagtgattctgatgaagagaatgatgctatggtttgtccaccagctgtctatgacaatactaccactgcaaatggtagtggttttcctttcaccactaatggtattggatatactgaaaggtaccaggaagatgccggcgttggtacaagtggccttggtttattgagtaacaatgttgatgattttgagatgaataactggcaaatgcattcttcttatcaacaacctgaacaaggcttccagttttttgggaatgatactgatgtccataatacttttgttggttcacacaattcctttggcttagcaccaaatgactattctcttgattgtaatgttggcgtagaggaggcttcggtaactcctgctctttcagtctgccggaatagtaatgaaatgcatggaagtttggttgataacccactggctttggttggcgatgatccatccttgcaaatttttcttccaagtcaaccttcctctgttcctcttcaggaagaacttagcgagcgtgctaatgcaccaaatggggttcagtctgatgattggatatcccttacactcgcagcgggtggaggtggtaacgaagagcctgcacctgctgatgtcaattcacagccacaaattccatcaacagagacagggatcgaaccattgaccgatgctgcttctgcatttctgagcacaaacattgaaagacgtagcggagctgatttaaatccaagaaggatagaaaatatattttctcatcctcgccagcctcggtctgttaggcctcgactctgtttatcaatagatactgattctgagtag</cdnaseq>|
AA = <aaseq>MADLVSSCKDKLAYFRIKELKDILNQLGLPKQGKKQDLIDRVLA LLTDEQGQRHHGWGRKNSLTKEAVAKIVDDTYRKMQIQCAPDLATRSHSGSDFSFRPI EEAYDSFQPEAKVRCICSSTMVNDSMIQCEDQRCQVWQHLNCVLIPDKPGESAEVPPV FYCELCRLSRADPFWVTAGNPLLPVKFVSSGVTNDGTSVPQSVEKSFQLSRSDRETVQ RQEYDLQVWCMLLNDKVQFRMQWPQYAELHVNGISVRVVTRPGSQLLGINGRDDGPLI TTCSREGINKICLSRVDARTFCFGVRIAKRRTVAQVLNLVPKEAEGESFEHALARVRR CLGGGDTAENADSDSDLEVVAESVTVNLRCPNSGSRMRIAGRFKPCIHMGCFDLETFV ELNQRSRKWQCPICLKNYSLESLMIDPYFNRITSLLRNCNEDVNEVDVKPDGSWRVKG DAASRELSQWHMPDGTLCNPKEDVKPAMQNGNEQMMEGTSDGQKSLKIGIKRNPNGIW EVSSKADDKKPSVVGNRMQNNSGFRALNNIMHMSNSPTSSYRDGEDPSVNQESNRHVD LSLNNGNNEFDSFSLNFGQACNTDDRPQQQHNATDVIVLSDSDEENDAMVCPPAVYDN TTTANGSGFPFTTNGIGYTERYQEDAGVGTSGLGLLSNNVDDFEMNNWQMHSSYQQPE QGFQFFGNDTDVHNTFVGSHNSFGLAPNDYSLDCNVGVEEASVTPALSVCRNSNEMHG SLVDNPLALVGDDPSLQIFLPSQPSSVPLQEELSERANAPNGVQSDDWISLTLAAGGG GNEEPAPADVNSQPQIPSTETGIEPLTDAASAFLSTNIERRSGADLNPRRIENIFSHP RQPRSVRPRLCLSIDTDSE</aaseq>|
DNA = <dnaseqindica>250..276#995..1075#1317..1359#1563..1641#1750..1909#2024..2151#2593..2661#3255..3342#3975..4050#4226..4299#6094..6201#7242..7403#7638..7742#7906..7986#8062..9262#10113..10258#aaaccacaacgaactaccccctcctcgtcgagccgacgcgagagaggaaagtgggttgcggcttgctgcgcgtgtggagtcgccattccccaattcgctgcgccgcccgccggatctcgtcttgccccctgcggcggcggtttgggcccccccttccgatcggtttcccccccgcacatggtcgtggcggcggcggaggtggtggtggtgcgggagtagggaggcgggcgaaccgaggcggcggcgccgatggcggacctggtttccagctgcaaggtagactcgcgttcccccgcccgccggtaaattcgaccccgatctccgccgattccgcggcgtaattcggcgccggggggggggaggggattcgttgcgtcgcgcgcgtctgttttggggcggggaaggttgcggcgtgcctggccccgcccttgctcgccgtgtttgcctgtagatgatgagatgattagttagttttgtcgatagttttagttttttttttccagtggttgctgtggagtttattagtgcctagctctctggcgtttagggttgcggattaatcggtgatttcagtatagcacctggcgaggggcttcactgggcagttcaattgcttaggctggcgctttcgcttgttggcaaatcccccacatttgcttgatccgtagtttgtcccatgctgtatggttttgtatcatacgggaagcatccagatgtttcatgtcatactaatcactaaataagttgctcagaaaattgctaattttcactttcttcagtttggtagtttgggtccttgtgttccatgacagttaatttagcatggttagttgcgtcatgcttaattccaggcaagagtatctctgctttgatttggaattgatcccagtatttagtccatggatcaaacgccagttgtaccttatgtatgtgtcatgccaaatgaattgctttggtaactttagcattgacccctttgcctttttcattgcaggataaactggcatactttagaataaaggaactcaaagatatattaaatcagctcggcttaccaaagcaaggaaagaagcaggtcagcaaattttgttcctgttaatcttttttacatcaccatctatcagacggcagtttcaactaaatgagaatatgagatgtaagcttttgaggtgaaccggtagtatagactctcataatacagcattggtgggatctttggtggtaatggtggtctgatatgaagagctcatttcaatgagctatttcttagctcgttgtcttttgttttctgatatacttctcttatattctcacaggatcttattgatagggtgttggcactcttaacagatgagcaaggtatgcatttgtctgttgtttctgcttccctggtctatgttagtaccttacatatcaagtcatgtactgacatgctaaaatttgcactgtttcattccctgtatttgtttcagtggtgtctaatccatcagcatgaacggatgatgccttttctattttctagtcttaaaaacctaaatttgtgtgttcttttcttgttgcaggtcaaaggcatcatggatggggaaggaaaaattctctcaccaaggaggcagtggcaaaaattgttgatgatacatacaggttaaatttcattgtttcataaaattgaccatgtggcataatagtgtggatatttgagatgaaatcagtggtgcttctcttttgagatctttatgccttcattagcaggaaaatgcaaatccaatgtgctcctgatctagccaccaggagccacagcggatcagatttcagtttcaggcctatagaggaagcctatgactctttccagccagaggccaaagttcgctgcatttgcagtagcacaatggttaatgacagcatgatccaggtattataacattttttatttcttacattcctttctttttgaaagatcgttacggcatgtgccatccctctttttattattattatttaaatcatgtatcatgtctttctgcagtgtgaagatcagcgatgccaagtgtggcaacatttgaattgtgtactcattccagataagcctggggagagcgctgaagttccacccgttttctactgtgaattatgccgactgagtcgggcagacccgtatgtctttctactatttgatttttctttgggtggggttaagcaggtaaatctgtggtggaaaccactgcattttttaaagaaaaatcaaaacaaaaacatatttagggaaggtgatagtacatgctcttgtaattccatgtgcaaacttgattttgcttgtgagatatgaaaatggaaaacagtgaacgaaaagacaaacatcaagaattcaagatgtgtggcttttaccttgttgtttctcaatttcatatctcattaatgaaattagttttgatcttctgatttcacacagtttaataatgtatcacctcctctgtgcatgagattacttggggattttttgaaacttgtacaaaatggtttccagtttatcaccatatattctacaaaatgggattaagttttgatttgctgcagctacatgctttgaatttgcagattttgggtcactgctggaaatccattactcccagtgaaattcgtgtcatctggtgttacaaatgatgggtgggtataaatggattcttcttactggcaacattttaactgataataatccgtggctgactatttatttattattgatgctcttctttctctatttcgtctttgcaaaaatggcagtttttccaacacctttatttacattcactggtagctcgctaggctctccctttcagtcactccttcaaatagaaaaacagttcatgacacttttttcatcatgcctcttaaatttttttcattgttgcacaatcatagaatgtttacagttacactacggctcataattacatacgtaggtaaaatccattgttgatacttgatagtcatgagtttactttgtcacatgcctctccatatattgtacaaggctcttcttagtagttacttgtcacattataataatccttgtaacatatggtaaattctccagttaacgttatctgtgactatacaacattttgtctagcacatttatggacaacagtatgcttagaaggttcaattgctattctatttactctaaattgtctcgtttaatgatcatgtgttacttgtgatgtatgacatccttattaatcctctcatatctttagaacaagtgtacctcaaagtgtggagaaaagcttccagctttctcgatcagatagagaaactgtccagagacaagaatatgacctccaggtttgttctattctacaaagctaagttaaatatgcttgagttttggagtcctcagttcaacttaatattgcgtcaatattgtaatatcataatgctcttgagtaccaagtaagaaaccatatgggtttatgccactcagttgtttaggacgttcataagtttttttgttgattttaggcttatatgaatagctcttatcaatggtgaaggcaaacctttgaccaattcattttccttttgtaacatggaattcacacaatatgagtaatgcgtctattgtgcaattgttttcattggttgtattgtgaagacaaagcactgtggataagttatgttctctatgtaacatgaaatattcttaatttgttctgttacaagccaactgtatttactttgtgttgttgcaaattttcatctgtaccatgtggatgctacttgctagcattgtacaaagaaattttctttgtgcaaaagtttgaatttatgtggaccaggctgatttcgaattcattttgtccaatgatgaaacatggtttaattcatttcgtattgtttttttccttgcataaatctaagatatgcatgcatatattgtttgtttatactatctgaagcagtgaatgtgttgacaggtttggtgcatgcttttgaatgacaaagttcagttcaggatgcagtggccccaatatgcagaattgcatgttaatggtattgttgatgattttgtagcacaatttgattcactttcatatttgaattgcatcccagcttaaatattgtcttatatgttgtgcagttatgctgttgttggttaatacttcacgttggtctttctttaaaataagttactatctgtgtaacattagtttcattttatgttcaggtatttctgtacgagtagtgactagacctggttctcaattactagggataaatggacgggatgatggtccactggtaagttcaatttccttctgtcacattggtaagatctagatactaattggcagagatttggctatcctatttgatattcctgtatgttattctattttaagttgtgccatttttgttgttctccggttgacagaacctaacgttattgagtggagttacaaccttaaaatggaaccatgttatgttacaaaacctttgggcttgttttcattgatctgtagttaaaatttgctttatcttctagtggcggcctgtaaaaatttgcttctgtatttttaatgctcctatgaaatctcctaaaatcatattcgcagtaactcttaggtggaaagtctcattgagaatcttagctggatgtgtttagtatgagaaaaatttactgcccagtttgaaacagattattgactagattattgtcatggattatttacatgatcatagatatagattaaatatttgataaataaacttaacgaatacagcatagaacaagggatctaaataatgcagta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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001061052.1 RefSeq:Os05g0125000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 5]]
[[Category:Chromosome 5]]

File:Untitled.png

2014-06-26T11:54:24Z

潘文波:

Os04g0530500

2014-05-29T10:55:42Z

潘文波:

OsRDCP1 was induced by drought stress. Overexpression of OsRDCP1 increased tolerance to drought stress in rice.

==Annotated Information==
===Function===
[[File:1-s2.0-S0168945211000641-gr4.jpg|right|thumb|300px|'''Figure 1.''' Protein In vitro self-ubiquitination analysis of OsRDCP1.]]

OsRDCP1 exhinit self-ubiquitination activity in vivo. MBP-OsRDCP1 produced high-molecular-mass smear ladders in a time-dependent manner ( Fig. 1A). In contrast, no ubiquitination was detected when the E1, E2, ATP, or Ub was excluded from the reaction mixture(Fig. 1).

[[File:1-s2.0-S0168945211000641-gr6.jpg|right|thumb|300px|'''Figure 6.''' Overexpression of OsRDCP1 increased tolerance to drought stress in transgenic rice plants.]]
35S:OsRDCP1 transgenic lines exhibited considerably
less visual symptoms of drought stress. After 15 days
of drought stress, these transgenic lines appeared
healthy and showed reduced desiccation damage
relative to the wild-type and osrtbp1 plants. while
no significant phenotypic differences were detected
between the wild-type and osrtbp1 plants.

===Homologues===
[[File:1-s2.0-S0168945211000641-gr1.jpg|left|thumb|300px|'''Figure 2.''' Protein Identification of five homologous rice RING E3 OsRDCP family members. ]]
These five rice ''OsRDCPs'' (O. sativa RING domain-containing proteins) paralogs
possess a single RING motif in their N-terminal
regions and a putative membrane-anchoring domain in
their C-terminal regions.The predicted OsRDCP
paralogs were 25–79% identical to each other at the
amino acid level .
among the five paralogs, OsRDCP1 was significantly induced by drought stress in both
shoot and root tissues, while the other OsRDCP
members were constitutively expressed ( Fig2). The
level of the OsRDCP4 transcript was the highest in
rice seedlings.

===Expression===
[[File:3456788.png|left|thumb|300px|'''Figure 3.''' RNA gel blot analyses of OsRDCP1 mRNA. Light-grown 2- or 4-week-
old rice plants . ]]
OsRDCP1 transcript (approximately
1.2 kb) levels were significantly augmented in
response to 5–30% water loss ( Fig. 3C). This
increase was seen in both shoots and roots with
similar degrees of induction. Significant induction
of OsRDCP1 transcripts was also detected following
cold stress (2, 6, and 24 h at 4 °C) ( Fig. 3D). In
contrast, expression of OsRDCP1 was unaffected by
high salinity (150 mM NaCl) for at least 24 h.

===Evolution===
[[File:Dfghj.png|left|thumb|300px|'''Figure 4.''' Phylogenetic relationship of RING Ub-ligase homologs from rice, Arabidopsis , hot pepper, and humans . ]]
The coding region of OsRDCP1
is 762 bp and encodes 253 amino acids with a
molecular mass of 28.1 kDa and a calculated pI of
7.2. Full-length OsRDCP1 is 37%, 29%, and 31%
identical to hot pepper CaRma1H1 [12], Arabidopsis
AtRma1, and human RING protein HsRma1, respectively (
Fig. 4). In addition, the RING domain of OsRDCP1 is
48–78% identical to those of hot pepper,
Arabidopsis, and human RING proteins.

You can also add sub-section(s) at will.

==Labs working on this gene==
Woo Taek Kim.Department of Systems Biology, College
of Life Science and Biotechnology, Yonsei University,
Seoul 120-749, Republic of Korea.

==References==
1. Hansol Bae;Sung Keun Kim;Seok Keun Cho;Bin Goo Kang;Woo Taek Kim Overexpression of OsRDCP1, a rice RING domain-containing E3 ubiquitin ligase, increased tolerance to drought stress in rice (Oryza sativa L.)
Plant Science, 2011, 180(6): 775-782

==Structured Information==
{{JaponicaGene|
GeneName = Os04g0530500|
Description = Zinc finger, RING-type domain containing protein|
Version = NM_001059925.1 GI:115459579 GeneID:4336483|
Length = 3313 bp|
Definition = Oryza sativa Japonica Group Os04g0530500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|
AP = Chromosome 4:26931200..26934512|
CDS = 26931390..26932151|
GCID = <gbrowseImage1>
name=NC_008397:26931200..26934512
source=RiceChromosome04
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008397:26931200..26934512
source=RiceChromosome04
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggaccagatttacatggctgctgtcaataacaagacttcattgcctgatgatgagccaatgaagaaaattagtggagatatgcctgtgactgctggaaacgcttgctttgactgcaacatttgccttgattttgcagcagagccggtggttactctttgtggtcatctctactgctggccttgcatctatgagtggctatgtccaggggttggatcgactgccagtaacaacagcagtttggcaaggcgacagtgccctgtgtgtaaggctacactctccccagacatgctggtgccattgtatggtcgtggggggagtctgaagaaatcactgaatggtgtgcccattccacgacgaccaaccgtgcagcgggaggcggttgagcatcaaaacacacacaataacatcgatgatcggcaccatgagaacatggaacccagccctccaccacaaccgctgcggcattcgagccatcattccagtgcaactgaatttgattttatttacccgccatcaccaattgggcgtggcttgatccactcgacggctggtggtgtgcttggggggatggcagtggcggtgctaccatgggcattccgtggccaggtgccaccgagcatgttcatgagtccccactatgtcacggcacacaatatgagctctagagcgaggagacatcagatggaagttgagaggtcactgcatcagatctggttcttcctcttcgtttttgtggtgctgtgcctgctcttgttctga</cdnaseq>|
AA = <aaseq>MDQIYMAAVNNKTSLPDDEPMKKISGDMPVTAGNACFDCNICLD FAAEPVVTLCGHLYCWPCIYEWLCPGVGSTASNNSSLARRQCPVCKATLSPDMLVPLY GRGGSLKKSLNGVPIPRRPTVQREAVEHQNTHNNIDDRHHENMEPSPPPQPLRHSSHH SSATEFDFIYPPSPIGRGLIHSTAGGVLGGMAVAVLPWAFRGQVPPSMFMSPHYVTAH NMSSRARRHQMEVERSLHQIWFFLFVFVVLCLLLF</aaseq>|
DNA = <dnaseqindica>2362..3123#cccgtctcgcctcctccgcaagtgacgtcgacgcctcatctcccctccctctttttccccgcagcgatacggcattcgtgacctcccgattgttgccaacccccctcgccttccgattgctgccacccccgcgcctcgccgcttgcccgtctcctgttcgtccctcgggagcgggaggctagggccgacagcagcgttcgcgccggcgcgggattgggttctttgttcttgtcaggtcagcgggatgaaatctcttgtttccttttgatgtttttttttgtttttgcccgaaagattttgcgttgtctggtcgattggaggaccggtgtgtgtcatgggggttgatctcggttggattcttaggttgctgcctgcccttgggttagctccgaaaggttctgatgctttgctgctgctgctcctcctggcttgtgctgttcgtgcttggggttgtgatgcctttgtccttctcgaggaacaagggaagatcgttgttagctctgtcggtggtctcctttcctttcctcggtttggccaattgtgtgttgatgcgcctgccgcactaggaaatcgacggtgctaaaatcgtttttctacccaatgtccaaccgagaaattgtttatgtttagatttagtgcagtatcaatttgaaccgcactagggaatcgacggtgctaaaatcgtttttttacccaatatccaacttccaaacttctaatttcgtttgtgtattgacgaatgcgtgccctgtgttagacatgagcagacagcatttggaggatttactaccatacacaaatctgaacaattttaacaaaatgtgttcgcactccaaaacatgatttatgatgtaatacactgtttctccacaacattgacttcttgtgtgaattcttaatggtgatagcggtggatttgttgtgtgaattcttaatggtgatagcggttgatgctgctgagcttagcagatccgtgtgcttgattattgtttcgtttcgtagtctcacattcaggatcagttattttgtttcttaaggctactcttattagccgtctatctaaattttaacactaggaatattgatgtgctcccacatttatctaaaatactcaatgtaacaaaacaacgtgtctgaatttgcgatagcctgagcttaagctcaccagttaataatattacacatgggatgagagcaatcaagaaatttcgaagatgctggtggacaaataattcagtgttccctgattctaatacagtatattgccattatctcttggttataattattttagtcggtcactagatatgtgaagtagtggtcttttcggtgtatttgatttaattcaatatgccaaatgatgtagctaatccatcattgttgtgtccacatcactttccacaccttgttttttttttcaataattcacttgtcgatggaccttatcaaccacataaagttcactatgtcatggaagcatttattggagattgggatgggatgcctctcaggcttagtcaactctgctggtttattcgtccatagctgcttacctgaatatttctatagaatcaattagaagagaggcagtattaatctagtcaggacctgccaggctgccatccttgtctaagccgccgtttgtaaactgtgggtgcatattaaatgctaactgttttgtactatgccttttcttcccaaggcatagtaactttaagttggcaatgatggttaatcttaatagtattgactttgcacacataggacataagctgtttcatttccttgctgacaaaagttccccatttatcctgatagataatacttgtagcgtagggataagaatgtcatttgtgataaaaaataaagggcagtgtatctagttcctattgaaaaaattggataatccattccatgactgctgactcagcctgctaggaaagatgatggattcttccttgatgtatcaattgattactaaccaggataatattaagtagtttttttctggcatatagacacggagttttggagctttacctaacatctgcaccactcttcagacttgttaaatgtatttggatagacttcgctggacaggatgatgtggtgtgggccaaaaagctgcattttacctggacccgcttagtttcatataaatctgttggtaccatgtggcttgcgttgttccttccattctcatttctggtgtgttttaagtttttatttctgtacaatgattagtaaagtgggccccactcagtgatgctttacttaactacatgtcatatgcattcctagtgacatatttttgtttgttgaactgcagtgctgtgtttgctcgcaagggtcatcgtgagaatggaccagatttacatggctgctgtcaataacaagacttcattgcctgatgatgagccaatgaagaaaattagtggagatatgcctgtgactgctggaaacgcttgctttgactgcaacatttgccttgattttgcagcagagccggtggttactctttgtggtcatctctactgctggccttgcatctatgagtggctatgtccaggggttggatcgactgccagtaacaacagcagtttggcaaggcgacagtgccctgtgtgtaaggctacactctccccagacatgctggtgccattgtatggtcgtggggggagtctgaagaaatcactgaatggtgtgcccattccacgacgaccaaccgtgcagcgggaggcggttgagcatcaaaacacacacaataacatcgatgatcggcaccatgagaacatggaacccagccctccaccacaaccgctgcggcattcgagccatcattccagtgcaactgaatttgattttatttacccgccatcaccaattgggcgtggcttgatccactcgacggctggtggtgtgcttggggggatggcagtggcggtgctaccatgggcattccgtggccaggtgccaccgagcatgttcatgagtccccactatgtcacggcacacaatatgagctctagagcgaggagacatcagatggaagttgagaggtcactgcatcagatctggttcttcctcttcgtttttgtggtgctgtgcctgctcttgttctgaagcaagatggtggtgtattccttttgtaaatacgggaaggcaagacactaatgggtggatgcatatggtaaaagagggtggttaacactgttggtctgtatcgataagcatgtgcctaaagcatgctgatatagagatgtaatgtgacaagacttaaggtgaaatgcacatttcattcttctcttgtcct</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001059925.1 RefSeq:Os04g0530500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 4]]
[[Category:Chromosome 4]]

File:1-s2.0-S0168945211000641-gr6.jpg

2014-05-29T10:49:23Z

潘文波:

File:1-s2.0-S0168945211000641-gr4.jpg

2014-05-29T10:46:13Z

潘文波:

File:Dfghj.png

2014-05-29T10:41:55Z

潘文波:

File:3456788.png

2014-05-29T10:38:41Z

潘文波:

File:1-s2.0-S0168945211000641-gr1.jpg

2014-05-29T10:33:29Z

潘文波:

Os01g0673600

2014-05-29T10:07:09Z

潘文波:

OsUBC13 can protect from spontaneous and environmental DNA damage. OsUbc13 catalyzes K63 polyubiquitination in vitro,suggesting that it is a housekeeping gene.

==Annotated Information==
===Function===
[[File:12284 2012 18 Fig3 HTML.jpg|right|thumb|300px|'''Figure 1.''' Physical interaction of OsUbc13 with Mms2/Uev1A from other species.]]
[[File:12284 2012 18 Fig4 HTML.jpg|right|thumb|300px|'''Figure 4.''' In vitro ubiquitin conjugation assays using purified proteins. ]]

OsUBC13 Functionally complements yeast ubc13 null mutant.OsUBC13 rescues the yeast ubc13 mutant from killing by MMS (alkylation damage), 4-nitroquinoline 1-oxide (4NQO, bulky lesions) and UV irradiation to a level comparable to that in wild-type cells.

OsUbc13 Physically interacts with yeast and human E2 variants((hUev1A and hMms2, yMms2 ).in pull-down assay (Figure 1). In E. coli cells co-transformed with plasmids expressing His6-OsUbc13 and GST-Uev, His6-OsUbc13 could be co-purified by GST-hUev1A (Lane 2) and GST-hMms2 (Lane 4) from total cell lysates. As a negative control, GST alone (Lane 6) was unable to pull-down His6-OsUbc13 under the same experimental conditions.all three Uevs from human and yeast can form stable heterodimers with OsUbc13 in vitro and in vivo.

OsUbc13, hUev1A and hMms2 were purified for an in vitro ubiquitination assay. As shown in Figure4, OsUbc13 (Lane 1), hUev1A (Lane 3) and hMms2 (Lane 7) alone did not promote free poly-ubiquitin chain formation. When both OsUbc13 and a Uev (hUev1A or hMms2) were present in the same reaction, di-Ub, tri-Ub and even longer Ub chains were readily formed (Lanes 4 and 8). Moreover, the assembly of free poly-Ub chains was not affected by the Ub-K48R mutation (Lanes 5 and 9); however, the Ub-K63R mutation completely abolished the Ub chain formation (Lanes 6 and 10). The above results indicate that with the assistance of hUev1A or hMms2, OsUbc13, can catalyze free poly-ubiquitin chain formation through K63 linkage.

===Expression===
[[File:12284 2012 18 Fig5 HTML.jpg|left|thumb|300px|'''Figure 2.''' Quantitative analyses of OsUBC13 expression. ]]

the expression of OsUBC13 (LOC_Os01g48280) remains high and remarkably stable during development (Figure 2) and in different tissues.abscisic acid (ABA), salicylic acid (SA) and jasmonic acid (JA) were selected to represent hormone treatments, while methyl methanesulfonate (MMS), cisplatin and hydrogen peroxide (H2O2) were chosen to represent DNA damage treatments. As shown in Figure 2b and quantitated by densitometry (data not shown), no obvious alteration was observed by any given treatments under our experimental conditions.

===Evolution===
[[File:12284 2012 18 Fig1 HTML.jpg|left|thumb|300px|'''Figure 3.''' Sequence analysis of OsUbc13 and its gene structure. ]]
OsUbc13 also shows a high degree of conservation with Ubc13s from other eukaryotic organisms. Moreover, critical amino acid residues defined in hUbc13, including Cys87 in the active site for Ub thioester formation , Met64 for the physical interaction with RING finger E3 ligases ,Glu55, Phe57 and Arg70, which form a “pocket” that determine the specific interaction with Mms2 , as well as a Q100 residue as the target of bacterial OspI deamidation, are all conserved in OsUbc13 (Figure 3a).

==Labs working on this gene==
'''Wei Xiao'''. Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, S7N 5E5, Canada.Email: wei.xiao@usask.ca

==References==
Zang et al.;Rice UBC13, a candidate housekeeping gene, is required for K63-linked polyubiquitination and tolerance to DNA damage
Rice, 2012, 5: 24

==Structured Information==
{{JaponicaGene|
GeneName = Os01g0673600|
Description = Similar to Ubiquitin-conjugating enzyme E2|
Version = NM_001050369.1 GI:115439108 GeneID:4325905|
Length = 3027 bp|
Definition = Oryza sativa Japonica Group Os01g0673600, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:29424431..29427457|
CDS = 29424710..29424747,29424839..29424936,29425195..29425268,29425348..29425389,29426179..29426237 ,29426888..29426974,29427089..29427116,29427271..29427306|
GCID = <gbrowseImage1>
name=NC_008394:29424431..29427457
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:29424431..29427457
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccaacagcaacctcccccggcgaatcatcaaggagacgcagcgactcctcagcgagccagcgccgggaatcagcgcgtctccgtcggaggagaacatgcgctacttcaacgtcatgatccttggcccggcacagtccccctatgaaggtggagtttttaagcttgaactctttttacctgaggaatatcctatggctgctccaaaggttaggttcctgaccaaaatataccaccccaacattgacaagcttggtaggatatgccttgacattctcaaggacaaatggagcccagcccttcagattcggacagttcttttgagtatccaggcactcctaagtgcaccaaaccctgatgatcctctctctgataacattgcaaagcactggaaagccaatgaagcagaagctgttgaaacagcaaaggagtggactcgcctgtatgccagcggtgcataa</cdnaseq>|
AA = <aaseq>MANSNLPRRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGP AQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQ IRTVLLSIQALLSAPNPDDPLSDNIAKHWKANEAEAVETAKEWTRLYASGA</aaseq>|
DNA = <dnaseqindica>2711..2748#2522..2619#2190..2263#2069..2110#1221..1279#484..570#342..369#152..187#ccccgtttcccccaccctcggctgacggctcctcctcctcctcctcctcttcctccctcccgatcccctcccccgcacgaaactcaaagcatccccggcgccgcagctccccggaggaggaagcccccgcgccccgccccgaccagatccgatggccaacagcaacctcccccggcgaatcatcaaggtcgcgcctccgatctgattgcctgggcgtagatctccccacgcctctaacccgattccccccttttcttttctttttttttttattttccctttctgtgctgattttttgtttttgctgtctgtgtgttcgcgtctggtttggatctgcgcaggagacgcagcgactcctcagcgagccaggtgcgggcggttttttttttttttatgtatcgcggattttggcgatggtgttgtgtttttccgtggtttgggttcgtgttgttctgatggttttgggttggtttgtttgtgcagcgccgggaatcagcgcgtctccgtcggaggagaacatgcgctacttcaacgtcatgatccttggcccggcacagtccccctatgaaggtacggcacccgatggatacgttatttgttgtagagggtgttacggctcccgatggatatgttatttgttgtagagggtggtcgattagggcgtttctaggtataccgcagaattgggtgattctgtataaacccaattactagaagtgttaattatgtgtcagtcatggacgggcacgcatttcgtaagcctgcttttgcttgattaggctggttcttttgcacttaccagttttagtttctcgggcggcgggcacttgcatgttctatgtgtctcatattccctttctagtatgaagttagtaatgctaaaacactagtgacttttagcaataatgttctaaactcggattatatacgtgacttataagtgcttttcttttatgtgttaacttgtttgatgctttgtgcggcaaaggttgcccttgattttccataaccaaattgatctcttcgatttatttgattagactaattatgagtattgatattgttggcttttcatgttgtggtgtataatttaaatatttcctttcttaaaccctttttgcgttaattcgggttggatatataatgggtgttaacagttagtagctttagatggtttattctattatcatttggcttcctgatattgttctttctatcaggtggagtttttaagcttgaactctttttacctgaggaatatcctatggctgctccaaaggtaacaagacatagataaactttggttatgttttatatgatattatggggactgtataccttcaagctaattatgtcgtatttagaattgcgtgtggttatatactcatattaaaagtcaggacatttctgcattcattgacttgtgttgtgaagcggagcattaatttggtgacattggactcatgcatataaccaaatacaggaaagtagctgaaacatttaatttgtttgaaccattctttggcaaaaaaaaatttttattctttatagcctaatttttagttacccatatttaatcgtgtcgctctggaaccatcgttggttttatatgttatatgtgatgtcttgttggtaatagttttgttacatttgcttgatttccttttagggataatgcaaaccctgcctatttgagatgtaatttgcaatgagatcctgtgccaaggaccaaggttgtaagatatacttttggtcctgtgggaacttttagctgtgtcctgagcatcagcacgccattgcttctcttgaccgtctttttgcttcaagttcttaaaggtgttcttaacatcacattgttcgtttggtgttcagccaaattataccttgattagttatttttttagtctgtttggcttgacaatgtcatagattggaagggtgaagaaatattactcatatgttatacaaccataccggattattgtctacttcagtctccctaagtgtaaagctatataattcattattgtctacctcagtctccattttttctttgttgcgtatgcaggttaggttcctgaccaaaatataccaccccaacattgacaaggtttgcagtcacattcctctttgttatttagtttacttcgtaatacattgtttattcattaataggtttacttttgtagcttggtaggatatgccttgacattctcaaggacaaatggagcccagcccttcagattcggacagttcttttgaggtctctcccccgttgcctgcacatgtgtattagctttttatatcctgcacaaattccttgtctgctaagctgttgtcttattacgattgacatgatttctgttgaatttgaattatttgttagcttacctgcatttagtaattttatcactgcccttgatgtgtttttccaacaaaaactcttgagcattgtcaactattggaagttaggggtatcactttgctatacttaccttgtgacaacactcttttttttcagtatccaggcactcctaagtgcaccaaaccctgatgatcctctctctgataacattgcaaagcactggaaagccaatgaagcagaagctgttgaaacaggtaaattgaagctagcaatccagttaacagtgtctcccctcacactctgattttattggttgcctgataagcgatggtctggcatttgcagcaaaggagtggactcgcctgtatgccagcggtgcataaaacccaatgcctctcgtgatgtaataacccgtcatgctttagccttaatcaaatgccatttgcttgataagaacaaactggagatattggcagtggaagggagtttaaatgactaccgcttgaattatttgtctgtatacatgtggttggttccctttaccctagggcacccttgactaatcagtatctgtggtagaacttggtgctcctccaatatatttattactttatgttggaaaacttaaattgttgaaattggatctttgttcgtgtggctgc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050369.1 RefSeq:Os01g0673600]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

Os01g0673600

2014-05-29T09:49:34Z

潘文波:

OsUBC13 can protect from spontaneous and environmental DNA damage. OsUbc13 catalyzes K63 polyubiquitination in vitro,suggesting that it is a housekeeping gene.

==Annotated Information==
===Function===
[[File:12284 2012 18 Fig1 HTML.jpg|right|thumb|300px|'''Figure 1.''' Physical interaction of OsUbc13 with Mms2/Uev1A from other species.]]
[[File:12284 2012 18 Fig4 HTML.jpg|right|thumb|300px|'''Figure 4.''' In vitro ubiquitin conjugation assays using purified proteins. ]]

OsUBC13 Functionally complements yeast ubc13 null mutant.OsUBC13 rescues the yeast ubc13 mutant from killing by MMS (alkylation damage), 4-nitroquinoline 1-oxide (4NQO, bulky lesions) and UV irradiation to a level comparable to that in wild-type cells.

OsUbc13 Physically interacts with yeast and human E2 variants((hUev1A and hMms2, yMms2 ).in pull-down assay (Figure 1). In E. coli cells co-transformed with plasmids expressing His6-OsUbc13 and GST-Uev, His6-OsUbc13 could be co-purified by GST-hUev1A (Lane 2) and GST-hMms2 (Lane 4) from total cell lysates. As a negative control, GST alone (Lane 6) was unable to pull-down His6-OsUbc13 under the same experimental conditions.all three Uevs from human and yeast can form stable heterodimers with OsUbc13 in vitro and in vivo.

OsUbc13, hUev1A and hMms2 were purified for an in vitro ubiquitination assay. As shown in Figure4, OsUbc13 (Lane 1), hUev1A (Lane 3) and hMms2 (Lane 7) alone did not promote free poly-ubiquitin chain formation. When both OsUbc13 and a Uev (hUev1A or hMms2) were present in the same reaction, di-Ub, tri-Ub and even longer Ub chains were readily formed (Lanes 4 and 8). Moreover, the assembly of free poly-Ub chains was not affected by the Ub-K48R mutation (Lanes 5 and 9); however, the Ub-K63R mutation completely abolished the Ub chain formation (Lanes 6 and 10). The above results indicate that with the assistance of hUev1A or hMms2, OsUbc13, can catalyze free poly-ubiquitin chain formation through K63 linkage.

===Expression===
[[File:12284 2012 18 Fig5 HTML.jpg|left|thumb|300px|'''Figure 2.''' Quantitative analyses of OsUBC13 expression. ]]

the expression of OsUBC13 (LOC_Os01g48280) remains high and remarkably stable during development (Figure 2) and in different tissues.abscisic acid (ABA), salicylic acid (SA) and jasmonic acid (JA) were selected to represent hormone treatments, while methyl methanesulfonate (MMS), cisplatin and hydrogen peroxide (H2O2) were chosen to represent DNA damage treatments. As shown in Figure 2b and quantitated by densitometry (data not shown), no obvious alteration was observed by any given treatments under our experimental conditions.

===Evolution===
[[File:12284 2012 18 Fig1 HTML.jpg|left|thumb|300px|'''Figure 3.''' Sequence analysis of OsUbc13 and its gene structure. ]]
OsUbc13 also shows a high degree of conservation with Ubc13s from other eukaryotic organisms. Moreover, critical amino acid residues defined in hUbc13, including Cys87 in the active site for Ub thioester formation , Met64 for the physical interaction with RING finger E3 ligases ,Glu55, Phe57 and Arg70, which form a “pocket” that determine the specific interaction with Mms2 , as well as a Q100 residue as the target of bacterial OspI deamidation, are all conserved in OsUbc13 (Figure 3a).

==Labs working on this gene==
'''Wei Xiao'''. Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, S7N 5E5, Canada.Email: wei.xiao@usask.ca

==References==
Zang et al.;Rice UBC13, a candidate housekeeping gene, is required for K63-linked polyubiquitination and tolerance to DNA damage
Rice, 2012, 5: 24

==Structured Information==
{{JaponicaGene|
GeneName = Os01g0673600|
Description = Similar to Ubiquitin-conjugating enzyme E2|
Version = NM_001050369.1 GI:115439108 GeneID:4325905|
Length = 3027 bp|
Definition = Oryza sativa Japonica Group Os01g0673600, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:29424431..29427457|
CDS = 29424710..29424747,29424839..29424936,29425195..29425268,29425348..29425389,29426179..29426237 ,29426888..29426974,29427089..29427116,29427271..29427306|
GCID = <gbrowseImage1>
name=NC_008394:29424431..29427457
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:29424431..29427457
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccaacagcaacctcccccggcgaatcatcaaggagacgcagcgactcctcagcgagccagcgccgggaatcagcgcgtctccgtcggaggagaacatgcgctacttcaacgtcatgatccttggcccggcacagtccccctatgaaggtggagtttttaagcttgaactctttttacctgaggaatatcctatggctgctccaaaggttaggttcctgaccaaaatataccaccccaacattgacaagcttggtaggatatgccttgacattctcaaggacaaatggagcccagcccttcagattcggacagttcttttgagtatccaggcactcctaagtgcaccaaaccctgatgatcctctctctgataacattgcaaagcactggaaagccaatgaagcagaagctgttgaaacagcaaaggagtggactcgcctgtatgccagcggtgcataa</cdnaseq>|
AA = <aaseq>MANSNLPRRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGP AQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQ IRTVLLSIQALLSAPNPDDPLSDNIAKHWKANEAEAVETAKEWTRLYASGA</aaseq>|
DNA = <dnaseqindica>2711..2748#2522..2619#2190..2263#2069..2110#1221..1279#484..570#342..369#152..187#ccccgtttcccccaccctcggctgacggctcctcctcctcctcctcctcttcctccctcccgatcccctcccccgcacgaaactcaaagcatccccggcgccgcagctccccggaggaggaagcccccgcgccccgccccgaccagatccgatggccaacagcaacctcccccggcgaatcatcaaggtcgcgcctccgatctgattgcctgggcgtagatctccccacgcctctaacccgattccccccttttcttttctttttttttttattttccctttctgtgctgattttttgtttttgctgtctgtgtgttcgcgtctggtttggatctgcgcaggagacgcagcgactcctcagcgagccaggtgcgggcggttttttttttttttatgtatcgcggattttggcgatggtgttgtgtttttccgtggtttgggttcgtgttgttctgatggttttgggttggtttgtttgtgcagcgccgggaatcagcgcgtctccgtcggaggagaacatgcgctacttcaacgtcatgatccttggcccggcacagtccccctatgaaggtacggcacccgatggatacgttatttgttgtagagggtgttacggctcccgatggatatgttatttgttgtagagggtggtcgattagggcgtttctaggtataccgcagaattgggtgattctgtataaacccaattactagaagtgttaattatgtgtcagtcatggacgggcacgcatttcgtaagcctgcttttgcttgattaggctggttcttttgcacttaccagttttagtttctcgggcggcgggcacttgcatgttctatgtgtctcatattccctttctagtatgaagttagtaatgctaaaacactagtgacttttagcaataatgttctaaactcggattatatacgtgacttataagtgcttttcttttatgtgttaacttgtttgatgctttgtgcggcaaaggttgcccttgattttccataaccaaattgatctcttcgatttatttgattagactaattatgagtattgatattgttggcttttcatgttgtggtgtataatttaaatatttcctttcttaaaccctttttgcgttaattcgggttggatatataatgggtgttaacagttagtagctttagatggtttattctattatcatttggcttcctgatattgttctttctatcaggtggagtttttaagcttgaactctttttacctgaggaatatcctatggctgctccaaaggtaacaagacatagataaactttggttatgttttatatgatattatggggactgtataccttcaagctaattatgtcgtatttagaattgcgtgtggttatatactcatattaaaagtcaggacatttctgcattcattgacttgtgttgtgaagcggagcattaatttggtgacattggactcatgcatataaccaaatacaggaaagtagctgaaacatttaatttgtttgaaccattctttggcaaaaaaaaatttttattctttatagcctaatttttagttacccatatttaatcgtgtcgctctggaaccatcgttggttttatatgttatatgtgatgtcttgttggtaatagttttgttacatttgcttgatttccttttagggataatgcaaaccctgcctatttgagatgtaatttgcaatgagatcctgtgccaaggaccaaggttgtaagatatacttttggtcctgtgggaacttttagctgtgtcctgagcatcagcacgccattgcttctcttgaccgtctttttgcttcaagttcttaaaggtgttcttaacatcacattgttcgtttggtgttcagccaaattataccttgattagttatttttttagtctgtttggcttgacaatgtcatagattggaagggtgaagaaatattactcatatgttatacaaccataccggattattgtctacttcagtctccctaagtgtaaagctatataattcattattgtctacctcagtctccattttttctttgttgcgtatgcaggttaggttcctgaccaaaatataccaccccaacattgacaaggtttgcagtcacattcctctttgttatttagtttacttcgtaatacattgtttattcattaataggtttacttttgtagcttggtaggatatgccttgacattctcaaggacaaatggagcccagcccttcagattcggacagttcttttgaggtctctcccccgttgcctgcacatgtgtattagctttttatatcctgcacaaattccttgtctgctaagctgttgtcttattacgattgacatgatttctgttgaatttgaattatttgttagcttacctgcatttagtaattttatcactgcccttgatgtgtttttccaacaaaaactcttgagcattgtcaactattggaagttaggggtatcactttgctatacttaccttgtgacaacactcttttttttcagtatccaggcactcctaagtgcaccaaaccctgatgatcctctctctgataacattgcaaagcactggaaagccaatgaagcagaagctgttgaaacaggtaaattgaagctagcaatccagttaacagtgtctcccctcacactctgattttattggttgcctgataagcgatggtctggcatttgcagcaaaggagtggactcgcctgtatgccagcggtgcataaaacccaatgcctctcgtgatgtaataacccgtcatgctttagccttaatcaaatgccatttgcttgataagaacaaactggagatattggcagtggaagggagtttaaatgactaccgcttgaattatttgtctgtatacatgtggttggttccctttaccctagggcacccttgactaatcagtatctgtggtagaacttggtgctcctccaatatatttattactttatgttggaaaacttaaattgttgaaattggatctttgttcgtgtggctgc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050369.1 RefSeq:Os01g0673600]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

File:12284 2012 18 Fig1 HTML.jpg

2014-05-29T09:22:51Z

潘文波: uploaded a new version of "File:12284 2012 18 Fig1 HTML.jpg"

File:12284 2012 18 Fig1 HTML.jpg

2014-05-29T09:22:45Z

潘文波:

File:12284 2012 18 Fig5 HTML.jpg

2014-05-29T09:19:30Z

潘文波:

File:12284 2012 18 Fig4 HTML.jpg

2014-05-29T09:15:37Z

潘文波:

File:12284 2012 18 Fig4 HTML.jpg

2014-05-29T09:15:37Z

潘文波: uploaded a new version of "File:12284 2012 18 Fig4 HTML.jpg"

File:12284 2012 18 Fig3 HTML.jpg

2014-05-29T09:10:55Z

潘文波:

Os03g0272300

2014-05-28T16:18:55Z

潘文波:

OsSDIR1 is a functional E3 ubiquitin ligase which plays crucial roles in the plant response to abiotic stress like drought and NaCl.

==Annotated Information==
===Function===
[[File:11103 2011 9775 Fig5 HTML.gif|right|thumb|350px| ''35S::OsSDIR1 can rescue the drought sensitivity of the Arabidopsis sdir1-1 mutant plants. '']]

OsSDIR1 was detected in all tissues of rice and up-regulated by drought and NaCl, but not by ABA. In vitro ubiquitination assays demonstrated that OsSDIR1 is a functional E3 ubiquitin ligase and that the RING finger region is required for its activity. OsSDIR1 could complement the drought sensitive phenotype of the sdir1 mutant and overexpressing transgenic Arabidopsis were more sensitive to ABA, indicating that the OsSDIR1 gene is a functional ortholog of SDIR1. Upon drought treatment, the OsSDIR1-transgenic rice showed strong drought tolerance compared to control plants. Analysis of the stomata aperture revealed that there were more closed stomatal pores in transgenic plants than those of control plants. This result was also confirmed by the water loss assay and leaf related water content (RWC) measurements during drought treatment.

===Expression===
[[File:11103 2011 9775 Fig2 HTML.gif|left|thumb|350px|''Tissue expression pattern of OsSDIR1.'' ]]

The expression of OsSDIR1 was detected in all tissues of rice, including leaves, stems, roots and spikes, and the highest level was found in the roots. the transcript level of OsSDIR1 was up-regulated by drought and NaCl. Upon drought treatment, the expression level of OsSDIR1 gene was slightly increased during the early period of the treatment time course, and an obvious induction was detected at 24 h. The induction by NaCl appeared very early, at 1 h after the start of the treatment and about a threefold level of induction was detected, reaching the highest at 4 h after which the expression returned back to normal levels. It is interesting that the expression of OsSDIR1 was suppressed by cold treatment, and the reduction of expression was observed from the 2-h time point, with almost no expression detectable from 8 h onward. No changes in expression levels were detected by the treatment with a stress-related hormone ABA at any point in the time course

===Localization===

[[File:11103 2011 9775 Fig3 HTML.gif|right|thumb|350px|''Intracellular membrane localization of the OsSDIR1 protein. '' ]]
Transient expression in onion epidermal cells demonstrated that GFP alone was found in both the cytosol and the nucleus, whereas GFP-OsSDIR1 was found densely localized in the plasma membrane and other intracellular membranes. The fluorescence was also detected on the membrane of the nucleus since the pattern appeared to be on the surface, and it is also different from the dense localization of GFP itself in the nucleus. Given the high similarity of amino acids between Arabidopsis SDIR1 and OsSDIR1, the OsSDIR1 protein could be localized on the intracellular membrane

===Evolution===
It was found that the SDIR1 protein is plant specific, and homologs could only be detected in plant species rather than in other organisms. SDIR1 homologs were found both in mono- and dicotyledonous plants. Only one rice homolog composed of 280 amino acids with 69% identity and 76% similarity to Arabidopsis SDIR1 was identified. It is interesting that four homologs were detected in the EST database from grape (Vitis vinifera), with identities of 72, 70, 68 and 57%. SDIR1 homologs all contain two putative transmembrane domains at the N-terminus and a C3H2C3 RING finger at the C-terminus of the protein.
[[File:11103 2011 9775 Fig1 HTML.gif|''Alignment of peptide sequences of plant SDIR1 homologs. Amino acid sequence analysis of Arabidopsis SDIR1 with putative homologous proteins from V. vinifera (CBI31650.3), Glycine max (ACU20874.1), O. sativa (ABF95226.1), Z. mays (NP_001131833), and S. bicolor (XP_002468109.1).'']]

==Labs working on this gene==
'''XieQi'''. State Key Laboratory of Plant Genomics, National Center
for Plant Gene Research, Institute of Genetics
and Developmental Biology, Chinese Academy of Sciences,
Datun Road, Beijing 100101, China
e-mail: qxie@genetics.ac.cn

==References==
1.Gao T1, Wu Y, Zhang Y. et al. OsSDIR1 overexpression greatly improves drought tolerance in transgenic rice.Plant Mol Biol. 2011 May;76(1-2):145-56. doi: 10.1007/s11103-011-9775-z

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0272300|
Description = Zinc finger, RING-type domain containing protein|
Version = NM_001056231.1 GI:115452190 GeneID:4332396|
Length = 3582 bp|
Definition = Oryza sativa Japonica Group Os03g0272300, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:9183459..9187040|
CDS = 9184249..9184296,9184389..9184617,9184706..9184841,9185005..9185053,9185680..9185805 ,9186560..9186673|
GCID = <gbrowseImage1>
name=NC_008396:9183459..9187040
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:9183459..9187040
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgttgttcatggtactgaactcacaccagatgtctccgaactttttggtttggctggttttgggtgtgttcctaatggccaccagcctaaggatgtacgccacatgccagcagctccaagcgcaggcgcaagctcacgctgcagctgccaacggcttccttggacacactgagctgcgggtgcatgtcccgccgaccatagctctcgccacacgaggccgtttgcagagccttaggctccaacttgcgctacttgaccgcgagttcgatgatttagattatgatgcccttagggccttggatgcagacaatagccctcatgctccttcgatgagtgaagaagaaataaatgctcttcctgtattcaaatacaaggttcaggcacatcagggaagtgcctctttccgaaaaagtgatggaccatctcaaccatctgtttcttctactgaatctggcaatgagaaaaagcaggatcgtttcaaggctgatgcaactgacaacaccctggaggatgagttgacatgcagtgtttgcttagaacaagtcgttgtgggtgatctattgagaagcctaccatgcctgcaccagtttcatgcaaactgcatcgatccatggttgcgccaacagggaacatgcccagtttgcaagcaccgtgtaagcgatgggtggcatggtgaagcagatgcttctaacatggtataa</cdnaseq>|
AA = <aaseq>MLFMVLNSHQMSPNFLVWLVLGVFLMATSLRMYATCQQLQAQAQ AHAAAANGFLGHTELRVHVPPTIALATRGRLQSLRLQLALLDREFDDLDYDALRALDA DNSPHAPSMSEEEINALPVFKYKVQAHQGSASFRKSDGPSQPSVSSTESGNEKKQDRF KADATDNTLEDELTCSVCLEQVVVGDLLRSLPCLHQFHANCIDPWLRQQGTCPVCKHR VSDGWHGEADASNMV</aaseq>|
DNA = <dnaseqindica>791..838#931..1159#1248..1383#1547..1595#2222..2347#3102..3215#actgctcgctctcgttttccccatccgcaccggctgctcctcgtcgcgccgacgcctccgcgcgccagcgaatcgccgcctcgagaggcccctcgtcgccgatcaggtgagtgcccctcctcgagagtcacgctcgttactcgattccgcggctgtttcaagatcaggcaggccctagaatctgggcgaaggttctcttgacttctggtagccttcgggggtttatctgggcgtttcccgcgttgaggcgtcatggacatgggagccgtgggggattttctaggtagggttttggttggggcgtcgctttcctcatattttgtcggtggctttgctgggcttcaggggcgtggcctcgtcgagtttttttgttcctcggaggggcggcaaggaggcggatttggagcaattcgtaagactagtggtggtggtggtggtggtggctggttggcgccatgagcttcgtgttccgggggagcagaggtgacatcgaggccggaggcttcccggggttcgccccggagcgccgcgccatggtgagcgtttctgcttaaatgtaggttttggcatcctaaagtagatctttttttgttatgaatttctgactcccatctgtgtgggggggcttgtatgcagcggattcatgcgggtggtcggccggtgaacagcaacctggcttttcttgtcactggtaatgtgggcatgccctttgtactaccgatattattaagctgtaaaaaatatcgattttctagttaatgcgatctattattctttttgggcagttcttatgttgttcatggtactgaactcacaccagatgtctccgaactttttggtaagttatcaattgatggtatgtattatgcttatatagcttgatggagaaatttcatgatttgtcgggttgcgcggaattgtctcttgtaggtttggctggttttgggtgtgttcctaatggccaccagcctaaggatgtacgccacatgccagcagctccaagcgcaggcgcaagctcacgctgcagctgccaacggcttccttggacacactgagctgcgggtgcatgtcccgccgaccatagctctcgccacacgaggccgtttgcagagccttaggctccaacttgcgctacttgaccgcgagttcgatgatttaggtgatacactttattcacttaggttaattcatatactgcttcttactctcctgcaagcattacattgtagctgatgttttgcttgcagattatgatgcccttagggccttggatgcagacaatagccctcatgctccttcgatgagtgaagaagaaataaatgctcttcctgtattcaaatacaaggttcaggcacatcagggaagtgcctctttccgaaaaaggtaagatgtattatccttttggctacattttttagctggcatagcaaacgcatgataagtctacaactgccttccatctctgataagtaaccaaccatataattccgaatttgtctactttgtatgggatgtaactaaccatttaattcttttgattctatagtgatggaccatctcaaccatctgtttcttctactgaatctggcaatgaggtaataaataaatactgacatgattatgaagaggttattctttgaatggcatctactgttattgtttgcataggcatgttgacatcctggcatgactaagataggagcatagaatgtgccatctatgtgttctttagcttgtattgatggaaatcattgagcgttactccctccgtcaacaaatataaaggattctaggtggtttagcagaatttaaggtcgaggtgaaattacttcgatgctgctagcagttttcttgattgggaaatatcacgcctagcggtgcaaccatcagtgagcaataaatacagtggttggatggatttggatagtagtactacaaaatctcttatatttgtggacatattttgaaccccaaaatccattatatttgtggaaagagtacttggttactttgcggttatttcatattgtggtggtatttcttagaattaaaatgcttttttgatgttcactatagtcaaccgtggtagctccataacaatcacaaagttgatgcttacacttttatttgtctattactcagcatgcctagaattacaatcagctgtgctgtttggtctggattatccttgttctagtgacttgttttttttgctgtgtagaaaaagcaggatcgtttcaaggctgatgcaactgacaacaccctggaggatgagttgacatgcagtgtttgcttagaacaagtcgttgtgggtgatctattgagaagcctaccatgcctgcaccaggtaatcattatactagaattatcttttttttagacatgtatactagaatcatcttgagcttgttttctttgtatgacagcaatagcaggctttatttcctagttatatctgctgtgctatgatcatggtgcctgttagcgtgttatatagtaactgtcctgacattttgttaaccaaatcccaaaacatgctaggaaaccactatatttgatgccatttgcaatttatgtgtgtttagtgaaaaagcaaagggaaaacttttgcatcatttcctatagctatcgtgtttcggaaaggaattgtttctttttccagtatgctaaatgctaagtatcttactctctcctgtttctgtaaggtgtggttcgagtgtttgactgtgatggtctttgatgttgaaccttggccactacacaaaatacgtgatctgtgcttggtttggctgcttaatcttgtattcaaatttgtaccggtctgagttccaaatatgtcatatatgctcacaatttttagtaaaatttaaatttgaatgaccatagccacatcaatgacaactatatactgtgttttgttatgttcgagttttcatagtgatacgatttatcctatatgagtgaaactatcatacactggctttttgctgttgtctttcaagtttttagctttccatcgaaggattgacattggtgctattagttttttgtgtcatgaggtgggactgcaaggtttacttactttcccacttgttttgcagtttcatgcaaactgcatcgatccatggttgcgccaacagggaacatgcccagtttgcaagcaccgtgtaagcgatgggtggcatggtgaagcagatgcttctaacatggtataagctgctgggagtggccagcaggtcttaagctaaaactactgacttaacccacggaatgaggattagctgtttagtcttgaggagtgtttttgtacataaaaaaacgttcacttctctgtgatgtttatgcatcaaaaggttgtgatatttttggttgacctgtaccctgttcagtatgaactcacatacaatcctttttactctgtttccgtggggaaattggcgtattatgggtgtttaaggctgaagatcagtagaggatttaagatccaagagtagtggtctggtctctattagtcttaagtaaaaatgatcttaactgttagtattatcacaagtaaaaaggagaaactgatttatgagttct</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001056231.1 RefSeq:Os03g0272300]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

File:11103 2011 9775 Fig5 HTML.gif

2014-05-28T16:02:52Z

潘文波: uploaded a new version of "File:11103 2011 9775 Fig5 HTML.gif"

File:11103 2011 9775 Fig5 HTML.gif

2014-05-28T16:02:51Z

潘文波:

File:11103 2011 9775 Fig4 HTML.gif

2014-05-28T15:59:05Z

潘文波:

File:11103 2011 9775 Fig3 HTML.gif

2014-05-28T15:58:19Z

潘文波:

File:11103 2011 9775 Fig1 HTML.gif

2014-05-28T15:32:30Z

潘文波:

File:11103 2011 9775 Fig2 HTML.gif

2014-05-28T15:32:19Z

潘文波:

OrsajCp110

2014-05-24T08:44:31Z

潘文波: Created page with "{{Plastid| GeneName = OrsajCp110 ORF137| Description =ORF137| Version = GeneID:3131497| Length = 414 bp| Definition = Oryza sativa Japonica Group ''OrsajCp110 ORF137'', Plasti..."

{{Plastid|
GeneName = OrsajCp110 ORF137|
Description =ORF137|
Version = GeneID:3131497|
Length = 414 bp|
Definition = Oryza sativa Japonica Group ''OrsajCp110 ORF137'', Plastid gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Plastid|Plastid]]|
AP = Plastid:133419..133832|
CDS =133419..133832|
GCID = <gbrowseImage3>
name=NC_001320:133419..133832
source=Rice_Japonica_Plastid
preset=GeneLocation
</gbrowseImage3>|
GSID = <gbrowseImage2>
name=NC_001320:133419..133832
source=Rice_Japonica_Plastid
preset=GeneLocation
</gbrowseImage2>|
AA = <aaseq>MGAFPSPPPWGWSTGFITTPLTTGRLPSQHLDPALPKLFWFTPTLPTCPTVAKQFWDTKRTSPDGNLKVADLPSFAISFATAPAALANCPPLPRVISMLCMAVPKGISVEVDSSFLSKNPFPNCTSFFQSIRLSRCI</aaseq>|
DNA = <dnaseqindica>1..414#ATGGGGGCTTTCCCTTCACCGCCCCCATGGGGGTGGTCCACAGGGTTCATAACTACCCCTCTTACTACGGGGCGTTTACCTAGCCAACACTTAGACCCGGCTCTACCCAAACTTTTTTGGTTCACCCCAACATTACCCACTTGTCCGACTGTTGCTAAGCAGTTTTGGGATACCAAACGGACCTCCCCAGATGGTAATCTTAAAGTGGCCGATTTACCTTCTTTTGCAATCAGTTTCGCTACAGCACCTGCTGCTCTAGCTAATTGCCCACCCCTTCCACGTGTGATTTCTATGTTATGTATGGCCGTGCCTAAGGGCATATCGGTTGAAGTAGATTCTTCTTTTCTCTCAAAAAACCCCTTCCCAAACTGTACAAGCTTCTTCCAAAGCATACGGCTTTCTAGATGTATATGA</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/gene/?term=Orsajcp110 NCBI GENE: Orsajcp110]
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Plastid]]
[[Category:Plastid]]

OrsajCp077

2014-05-24T08:33:12Z

潘文波:

{{Plastid|
GeneName = OrsajCp077 ORF72|
Description =ORF72|
Version = GeneID:3131480|
Length = 216 bp|
Definition = Oryza sativa Japonica Group ''OrsajCp077 ORF72'', Plastid gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Plastid|Plastid]]|
AP = Plastid:90227..90442|
CDS =90227..90442|
GCID = <gbrowseImage3>
name=NC_001320:90227..90442
source=Rice_Japonica_Plastid
preset=GeneLocation
</gbrowseImage3>|
GSID = <gbrowseImage2>
name=NC_001320:90227..90442
source=Rice_Japonica_Plastid
preset=GeneLocation
</gbrowseImage2>|
AA = <aaseq>MNFIVIEIHVLPRQNFELAILLPNRQRLTSVERMIHSDRYEDPTTLHCRIHVPYLKRVDLCASLMVQSSSC</aaseq>|
DNA = <dnaseqindica>1..216#ATGAATTTCATAGTAATAGAAATCCATGTCCTACCGAGACAGAATTTCGAACTTGCTATCCTCTTGCCTAATAGGCAAAGATTGACCTCTGTAGAAAGAATGATTCATTCGGATCGATATGAGGACCCAACTACGTTGCATTGCAGAATCCATGTTCCATATTTGAAGAGGGTTGACCTCTGTGCTTCTCTCATGGTACAATCCTCTTCCTGCTGA</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/gene/?term=OrsajCp077 NCBI GENE: OrsajCp077]
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Plastid]]
[[Category:Plastid]]

OrsajCp077

2014-05-24T08:31:19Z

潘文波: Created page with "{{Plastid| GeneName = OrsajCp077 ORF72| Description =ORF72| Version = GeneID:3131480| Length = 216 bp| Definition = Oryza sativa Japonica Group ''OrsajCp077 ORF72'', Plastid g..."

{{Plastid|
GeneName = OrsajCp077 ORF72|
Description =ORF72|
Version = GeneID:3131480|
Length = 216 bp|
Definition = Oryza sativa Japonica Group ''OrsajCp077 ORF72'', Plastid gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Plastid|Plastid]]|
AP = Plastid:90227..90442|
CDS =90227..90442|
GCID = <gbrowseImage3>
name=NC_001320:90227..90442
source=Rice_Japonica_Plastid
preset=GeneLocation
</gbrowseImage3>|
GSID = <gbrowseImage2>
name=NC_001320:90227..90442
source=Rice_Japonica_Plastid
preset=GeneLocation
</gbrowseImage2>|
AA = <aaseq>MNFIVIEIHVLPRQNFELAILLPNRQRLTSVERMIHSDRYEDPTTLHCRIHVPYLKRVDLCASLMVQSSSC</aaseq>|
DNA = <dnaseqindica>1..216#ATGAATTTCATAGTAATAGAAATCCATGTCCTACCGAGACAGAATTTCGAACTTGCTATCCTCTTGCCTAATAGGCAAAGATTGACCTCTGTAGAAAGAATGATTCATTCGGATCGATATGAGGACCCAACTACGTTGCATTGCAGAATCCATGTTCCATATTTGAAGAGGGTTGACCTCTGTGCTTCTCTCATGGTACAATCCTCTTCCTGCTGA</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/gene?term=3131418 NCBI Gene:psbL]
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Plastid]]
[[Category:Plastid]]

Os10g0573450

2014-05-24T08:22:38Z

潘文波: /* Function */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Similar to Chloroplast 50S ribosomal protein L2

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0573450|
Description = Similar to Chloroplast 50S ribosomal protein L2|
Version = NM_001189356.1 GI:297727842 GeneID:9269991|
Length = 1272 bp|
Definition = Oryza sativa Japonica Group Os10g0573450, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:23225998..23227269|
CDS = 23225998..23226049,23226713..23227121|
GCID = <gbrowseImage1>
name=NC_008403:23225998..23227269
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:23225998..23227269
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>aggagaatacttaataatacggcgaaacatttatacaaaacacctatcccgagcacacgcaagggaaccatagataggcaagtgaaatccaatccacgaaataatttgatccatggacggcaccgttgtggtaaaggtcgtaattccagaggaatcattaccgcaaggcatagagggggaggtcataagcgcctataccgtaaaatcgattttcgacggaatcaaaaagacatatctggtagaatcgtaaccatagaatacgaccctaatcgaaatgcgtacatttgtctcatacactatggggatggtgagaagggatatattttacatcccagaggggctataattggagatactattgtttctggtacaaaagttcctatatcaatgggaaatgccctacctttgaccgatatgcccttaggcacggccatacataacatagaaatcacacgtggaag</cdnaseq>|
AA = <aaseq>RRILNNTAKHLYKTPIPSTRKGTIDRQVKSNPRNNLIHGRHRCG KGRNSRGIITARHRGGGHKRLYRKIDFRRNQKDISGRIVTIEYDPNRNAYICLIHYGD GEKGYILHPRGAIIGDTIVSGTKVPISMGNALPLTDMPLGTAIHNIEITRG</aaseq>|
DNA = <dnaseqindica>1221..1272#149..557#taaggtagtagctgtgaatagccatcgactgcccggaaagggtagaagaatgggccctattctaggacatacaatgcattacagacgtatgatcattacccttcaaccgggttattctattccacttctagatagagaaaaaaactaaaggagaatacttaataatacggcgaaacatttatacaaaacacctatcccgagcacacgcaagggaaccatagataggcaagtgaaatccaatccacgaaataatttgatccatggacggcaccgttgtggtaaaggtcgtaattccagaggaatcattaccgcaaggcatagagggggaggtcataagcgcctataccgtaaaatcgattttcgacggaatcaaaaagacatatctggtagaatcgtaaccatagaatacgaccctaatcgaaatgcgtacatttgtctcatacactatggggatggtgagaagggatatattttacatcccagaggggctataattggagatactattgtttctggtacaaaagttcctatatcaatgggaaatgccctacctttgagtgcggtttgaactattgatttacgtaattggaagtaaccaattaggtttacgacgaaacctagaaatcgatcactgattcaatttgactacctctacaggatagacctcaacagaaaactgttgagtaacggcagcaagtgattgagttcaggagttcctcatagaaaattattgactctagagatatggtaatatggagaagacaaaattgtttgaagcacgcacagaaccggaagcgccccttgtttcaaagagaggaggacgggttattcacatttaatttgatggtcagaggcgaattgaaagctaagcagtggtaattaagacccccgggggaaaatagggatgtctcctacgttacccataatatgtagaagtatcgacgtaatttcatagagtcattcgatctgaatgctacatgaagaacataagccagatgacggaacgcggagacctagaatgtagaagatcataacatgagcgattcggcatatttggattactttcctatatatccactcatgtggtacttcatcatacgattcatataagatccatctgtctagagatcgtcatattcatctagaaagccgtatgctttggaagaagcttgtacagtttgggaaggggttttttgagagaaaagaagaatctacttcaaccgatatgcccttaggcacggccatacataacatagaaatcacacgtggaag</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001189356.1 RefSeq:Os10g0573450]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

OrsajCp070

2014-05-24T08:09:43Z

潘文波: Created page with "{{Plastid| GeneName = OrsajCp070 ORF137| Description = ORF137| Version = GeneID:3131477| Length = 414bp| Definition = Oryza sativa Japonica Group ''OrsajCp070 ORF137'', Plasti..."

{{Plastid|
GeneName = OrsajCp070 ORF137|
Description = ORF137|
Version = GeneID:3131477|
Length = 414bp|
Definition = Oryza sativa Japonica Group ''OrsajCp070 ORF137'', Plastid gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Plastid|Plastid]]|
AP = Plastid:81286..81699|
CDS = 81286..81699|
GCID = <gbrowseImage3>
name=NC_001320:81286..81699
source=Rice_Japonica_Plastid
preset=GeneLocation
</gbrowseImage3>|
GSID = <gbrowseImage2>
name=NC_001320:81286..81699
source=Rice_Japonica_Plastid
preset=GeneLocation
</gbrowseImage2>|
AA = <aaseq>MGAFPSPPPWGWSTGFITTPLTTGRLPSQHLDPALPKLFWFTPTLPTCPTVAKQFWDTKRTSPDGNLKVADLPSFAISFATAPAALANCPPLPRVISMLCMAVPKGISVEVDSSFLSKNPFPNCTSFFQSIRLSRCI</aaseq>|
DNA = <dnaseqindica>1..414#ATGGGGGCTTTCCCTTCACCGCCCCCATGGGGGTGGTCCACAGGGTTCATAACTACCCCTCTTACTACGGGGCGTTTACCTAGCCAACACTTAGACCCGGCTCTACCCAAACTTTTTTGGTTCACCCCAACATTACCCACTTGTCCGACTGTTGCTAAGCAGTTTTGGGATACCAAACGGACCTCCCCAGATGGTAATCTTAAAGTGGCCGATTTACCTTCTTTTGCAATCAGTTTCGCTACAGCACCTGCTGCTCTAGCTAATTGCCCACCCCTTCCACGTGTGATTTCTATGTTATGTATGGCCGTGCCTAAGGGCATATCGGTTGAAGTAGATTCTTCTTTTCTCTCAAAAAACCCCTTCCCAAACTGTACAAGCTTCTTCCAAAGCATACGGCTTTCTAGATGTATATGA</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/gene/?term=OrsajCP070 NCBI Gene:OrsajCp070 ORF137 ]
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Plastid]]
[[Category:Plastid]]

OrsajCp074

2014-05-24T08:02:21Z

潘文波: Created page with "{{Plastid| GeneName = OrsajCp074 ORF249| Description = ORF249| Version = GeneID:3131479| Length = 750 bp| Definition = Oryza sativa Japonica Group ''OrsajCp074 ORF249'', Plast..."

{{Plastid|
GeneName = OrsajCp074 ORF249|
Description = ORF249|
Version = GeneID:3131479|
Length = 750 bp|
Definition = Oryza sativa Japonica Group ''OrsajCp074 ORF249'', Plastid gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Plastid|Plastid]]|
AP = Plastid:83997..84746|
CDS = 83997..84746|
GCID = <gbrowseImage3>
name=NC_001320:83997..84746
source=Rice_Japonica_Plastid
preset=GeneLocation
</gbrowseImage3>|
GSID = <gbrowseImage2>
name=NC_001320:83997..84746
source=Rice_Japonica_Plastid
preset=GeneLocation
</gbrowseImage2>|
AA = <aaseq>MTKDETLLVFTLVVSSVSVFLFGILLFMVLISATRDFRERTKSKLVKIMIWAGIVVITFAIAVRIYPIFIFLLKERIKPLVEALYDKLPWIWEVSLSRYWDRLIDFLDRYLWACAQRIQTGIRKQKGEFVVTFSCRVKKRLYARAIEVGIHLSLLSNLFWILKTTLAVGYRLLWVLYYIISFEGFLGSFRLYLVYFGFYCLLFSGKWLRTSEDRGERQAEISGILLRGMLIECAFSVLCLEEDSNLHAL</aaseq>|
DNA = <dnaseqindica>1..750#ATGACAAAAGACGAGACTCTACTAGTCTTCACTCTTGTGGTTTCCTCGGTTTCTGTTTTCTTATTCGGGATCTTGCTTTTCATGGTTCTCATCTCTGCAACTCGCGATTTTCGCGAGAGAACCAAATCCAAGTTGGTGAAGATCATGATTTGGGCTGGCATAGTAGTGATTACCTTTGCAATTGCGGTTCGAATCTATCCGATCTTTATCTTTTTGCTCAAAGAACGAATAAAACCCCTTGTCGAAGCCCTTTATGATAAGCTTCCCTGGATCTGGGAAGTTTCTCTTTCACGGTATTGGGATCGTTTGATCGATTTCCTTGATCGCTACTTATGGGCGTGCGCTCAAAGGATACAAACAGGGATTCGCAAACAAAAAGGGGAATTCGTAGTCACTTTTTCCTGTCGCGTAAAAAAAAGGCTTTACGCGAGAGCAATAGAGGTTGGGATACATCTATCTCTTCTGAGCAACCTCTTTTGGATTCTTAAGACCACCCTTGCAGTAGGATACCGTCTGCTTTGGGTTCTTTATTATATTATCTCCTTCGAGGGATTTTTAGGATCGTTCAGGCTATATTTAGTCTATTTTGGCTTTTACTGTCTCCTTTTCTCAGGGAAGTGGTTAAGGACCTCAGAAGATAGAGGAGAGCGCCAGGCCGAGATTTCCGGAATACTTCTACGGGGAATGCTCATTGAATGCGCATTCTCCGTATTATGCCTTGAAGAGGACTCGAACCTCCACGCTCTTTAG</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/gene/?term=OrsajCP074 NCBI Gene:OrsajCp074 ORF249]
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Plastid]]
[[Category:Plastid]]

OrsajCp011

2014-05-24T06:50:21Z

潘文波:

{{Plastid|
GeneName = OrsajCp011 ORF91 |
Description = ORF100|
Version = GeneID:3131453|
Length = 270 bp|
Definition = Oryza sativa Japonica Group ''unnamed protein product; ORF91 '', Plastid gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Plastid|Plastid]]|
AP = Plastid:14077..14346|
CDS = 14077..14346|
GCID = <gbrowseImage3>
name=NC_001320:14077..14346|
source=Rice_Japonica_Plastid
preset=GeneLocation
</gbrowseImage3>|
GSID = <gbrowseImage2>
name=NC_001320:14077..14346|
source=Rice_Japonica_Plastid
preset=GeneLocation
</gbrowseImage2>|
AA = <aaseq> MVYGYGKSNM PHPNRKRKGT DTQYDYWEEL LVMVSGLYVL FCVFLVLFIF FDSFKQESNK
LELSGKEEKK KLNGENRLSR DIQNLLYIK</aaseq>|
DNA = <dnaseqindica>1..270#ATGGTATATGGATATGGAAAATCCAACATGCCCCATCCTAATAGGAAAAGGAAGGGAACAGATACCCAAT
ATGATTATTGGGAAGAACTTTTGGTAATGGTCTCGGGCCTATATGTTCTTTTTTGTGTGTTCTTAGTTCT
ATTCATCTTCTTTGATTCTTTTAAACAAGAATCTAATAAACTAGAATTGAGTGGAAAAGAAGAGAAGAAA
AAGTTAAACGGAGAGAATCGACTAAGCAGAGACATTCAGAATCTTCTTTACATCAAGTAA</dnaseqindica>|
Link = http://www.ncbi.nlm.nih.gov/gene/?term=OrsajCp011 NCBI Gene:OrsajCp011 ORF91
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Plastid]]
[[Category:Plastid]]

OrsajCp012

2014-05-24T06:49:28Z

潘文波: Created page with "{{Plastid| GeneName = OrsajCp012 ORF70 | Description = ORF70| Version = GeneID:3131454| Length = 213 bp| Definition = Oryza sativa Japonica Group ''unnamed protein product; OR..."

{{Plastid|
GeneName = OrsajCp012 ORF70 |
Description = ORF70|
Version = GeneID:3131454|
Length = 213 bp|
Definition = Oryza sativa Japonica Group ''unnamed protein product; ORF70 '', Plastid gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Plastid|Plastid]]|
AP = Plastid:14571..14783|
CDS = 14571..14783|
GCID = <gbrowseImage3>
name=NC_001320:14571..14783|
source=Rice_Japonica_Plastid
preset=GeneLocation
</gbrowseImage3>|
GSID = <gbrowseImage2>
name=NC_001320:14571..14783|
source=Rice_Japonica_Plastid
preset=GeneLocation
</gbrowseImage2>|
AA = <aaseq> MKTFSIYVLF PLIGWGINEF APFIYPYGWV LIFSDILLVC FGERLKQFLT GIPWKNLRVQ
NFAIFLKRIC </aaseq>|
DNA = <dnaseqindica>1..213#ATGAAAACTTTCTCCATTTATGTTTTATTTCCTCTAATTGGGTGGGGTATAAACGAATTCGCGCCCTTCA
TATACCCATATGGCTGGGTATTAATTTTCAGTGATATACTTCTTGTCTGTTTTGGTGAGAGGTTGAAACA
ATTTTTAACAGGCATCCCTTGGAAAAACCTTCGAGTTCAAAACTTTGCAATTTTTCTTAAGCGGATTTGT
TGA</dnaseqindica>|
Link = http://www.ncbi.nlm.nih.gov/gene/?term=OrsajCp012 NCBI Gene:OrsajCp012 ORF70
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Plastid]]
[[Category:Plastid]]

OrsajCp011

2014-05-24T06:42:02Z

潘文波: Created page with "{{Plastid| GeneName = OrsajCp0011 ORF91 | Description = ORF100| Version = GeneID:3131453| Length = 270 bp| Definition = Oryza sativa Japonica Group ''unnamed protein produc..."

{{Plastid|
GeneName = OrsajCp0011 ORF91 |
Description = ORF100|
Version = GeneID:3131453|
Length = 270 bp|
Definition = Oryza sativa Japonica Group ''unnamed protein product; ORF91 '', Plastid gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Plastid|Plastid]]|
AP = Plastid:14077..14346|
CDS = 14077..14346|
GCID = <gbrowseImage3>
name=NC_001320:14077..14346|
source=Rice_Japonica_Plastid
preset=GeneLocation
</gbrowseImage3>|
GSID = <gbrowseImage2>
name=NC_001320:14077..14346|
source=Rice_Japonica_Plastid
preset=GeneLocation
</gbrowseImage2>|
AA = <aaseq> MVYGYGKSNM PHPNRKRKGT DTQYDYWEEL LVMVSGLYVL FCVFLVLFIF FDSFKQESNK
LELSGKEEKK KLNGENRLSR DIQNLLYIK</aaseq>|
DNA = <dnaseqindica>1..270#ATGGTATATGGATATGGAAAATCCAACATGCCCCATCCTAATAGGAAAAGGAAGGGAACAGATACCCAAT
ATGATTATTGGGAAGAACTTTTGGTAATGGTCTCGGGCCTATATGTTCTTTTTTGTGTGTTCTTAGTTCT
ATTCATCTTCTTTGATTCTTTTAAACAAGAATCTAATAAACTAGAATTGAGTGGAAAAGAAGAGAAGAAA
AAGTTAAACGGAGAGAATCGACTAAGCAGAGACATTCAGAATCTTCTTTACATCAAGTAA</dnaseqindica>|
Link = http://www.ncbi.nlm.nih.gov/gene/?term=OrsajCp011 NCBI Gene:OrsajCp011 ORF91
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Plastid]]
[[Category:Plastid]]

ORF100

2014-05-24T06:32:06Z

潘文波: Created page with "{{Plastid| GeneName = OrsajCp007 ORF100 | Description = ORF100| Version = GeneID:3131451| Length = 303 bp| Definition = Oryza sativa Japonica Group ''unnamed protein produc..."

{{Plastid|
GeneName = OrsajCp007 ORF100 |
Description = ORF100|
Version = GeneID:3131451|
Length = 303 bp|
Definition = Oryza sativa Japonica Group ''unnamed protein product; ORF100 '', Plastid gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Plastid|Plastid]]|
AP = Plastid:8349..8651|
CDS = 8349..8651|
GCID = <gbrowseImage3>
name=NC_001320:8349..8651
source=Rice_Japonica_Plastid
preset=GeneLocation
</gbrowseImage3>|
GSID = <gbrowseImage2>
name=NC_001320:8349..8651
source=Rice_Japonica_Plastid
preset=GeneLocation
</gbrowseImage2>|
AA = <aaseq> MKLYKWIFLY FLRLRPRKAR ISRYLLFHIL VTRCSIGIRR NRNPFPLHIK MDFESQFFFS
ISLLFFRILF LFLYPCNRER VGKGRLLFFI FSLKNRLSWK </aaseq>|
DNA = <dnaseqindica>1..303#ATGAAATTGTATAAGTGGATTTTTTTGTATTTCCTTAGACTTAGACCACGCAAGGCAAGAATTTCTCGCT
ATTTACTATTTCATATTCTTGTTACTAGATGTTCTATAGGAATAAGAAGAAATCGCAACCCCTTTCCGCT
ACACATAAAAATGGATTTCGAAAGTCAATTTTTCTTTTCAATATCTTTACTTTTTTTCAGAATCCTATTT
TTGTTCTTATACCCATGCAATAGAGAGCGAGTGGGAAAAGGGAGGTTACTTTTTTTCATTTTTTCCTTAA
AAAATAGGCTTTCTTGGAAATAG</dnaseqindica>|
Link = http://www.ncbi.nlm.nih.gov/gene/?term=OrsajCp007 NCBI Gene:OrsajCp007 ORF100
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Plastid]]
[[Category:Plastid]]

Os01g0197700

2014-05-23T10:31:48Z

潘文波:

Gn1a, is a gene for cytokinin oxidase/dehydrogenase (OsCKX2). Reduced expression of OsCKX2 causes cytokinin accumulation in inflorescence meristems and increases the number of reproductive organs, resulting in enhanced grain yield.

==Annotated Information==
===Function===
[[File:Eeee.jpg|right|thumb|350px| ''
QTL analysis and molecular cloning. (A) Gross morphology of Koshihikari and Habataki at maturity.(D) Comparison of grain number in the main panicle of Koshihikari (Ko), Habataki (Ha), and 5150. (H) High-resolution linkage map of Gn1a. (I) OsCKX2 structure and mutation sites in Habataki (blue) and 5150 (red). (K) Comparison of grain number per main panicle in nontransgenic and transgenic lines. 2 copy CKX2, transgenic <ref name="ref1" />).'']]
Transgenic plants carrying two copies of the sense strand of OsCKX2 that was highly expressed showed reduced grain numbers compared to TC65. However, transgenic plants with antisense strands of OsCKX2 that had reduced levels of expression developed higher grain numbers.
OsCKX2 reduce CKX activity in Habataki, NIL-Gn1a, and 5150, and the increased production of CK conjugates to reduce the overall CK activity .

===Protein Structure===
[[File:搜狗截图14年05月23日1804 1.png|left|thumb|550px| ''Conserved domains on OsCKX2.'']]

The OsCKX2 of Koshihikari and Habataki consist of four exons and three introns and encode proteins of 565 or 563 amino acids, respectively. the region between 74 – 255 is FAD-binding PCMH-type domian. it has UDP-N-acetylmuramate dehydrogenase activity, cytokinin dehydrogenase activity and flavin adenine dinucleotide binding.<ref name="ref2" />

===Expression===
[[File:Dfghjj.png|left|thumb|150px| ''Expression profile of OsCKX2.'']]
transgenic rice harboring an OsCKX2 promoter::β-glucuronidase (GUS) construct. GUS expression was observed mainly in the vascular tissue in developing culms, inflorescence meristems, and young flowers in the T2 generation of transgenic plants . The expression of OsCKX2 in inflorescence meristems might regulate the CK level to control flower number. CK is known to be translocated acropetally via the xylem and systemically via the phloem . The high levels of expression in these tissues suggest that OsCKX2 plays a role in regulating CK levels in the vascular system of developing culms, where CK is transported to the inflorescence meristems<ref name="ref1" />.

===Evolution===
[[File:Fggg.png|right|thumb|250px| ''Phylogenetic relationship of CKX proteins in rice and Arabidopsis.'']]
Phylogenetic relationship of CKX proteins in rice and Arabidopsis.<ref name="ref1" />

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1">
Ashikari, M., Sakakibara, H., Lin, S., Yamamoto, T., Takashi, T., Nishimura, A., ... & Matsuoka, M. (2005). Cytokinin oxidase regulates rice grain production. Science, 309(5735), 741-745.</ref>
<ref name="ref2">
http://www.uniprot.org/uniprot/Q4ADV8</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os01g0197700|
Description = OsCKX2，cytokinin oxidase/dehydrogenase|
Version =AB205193.1 GI:71609872.(gene version=Gene ID: 4327334)|
Length = 1698 bp|
Definition = Oryza sativa Japonica Group OsCKX2 mRNA for cytokinin oxidase/dehydrogenase, complete cds.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:5273310..5274501|
CDS = 1..625,717..1192|
GCID = <gbrowseImage1>
name=NC_008394:5273310..5274501
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:5273310..5274501
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgaagcaagagcaggtcaggatggcagtgctcctcatgctcaactgcttcgtcaaggccacggcgccgccgccatggccgccgtcggcttcgtccgcctccttcctcgacgacctcggcgacctcggcatcgcgccgctcatccgcgccgacgaggcgggcaccgcgcgcgcctccgccgactttggcaacctctccgtcgccggcgtcggggcgcctcggctcgccgccgccgccgccgtgctctacccgtcgcgccccgccgacatcgccgcgctgctgcgcgcgtcgtgcgcacgcccggcgccgttcgcggtgtccgcgcgggggtgtggccactcggtgcacggccaggcctccgcgcccgacggcgtcgtcgtcgacatggcgtcgctcggccgcctgcagggcggcggcgcgcggcgcctcgccgtgtcagtggaggggcggtacgtcgacgccggcggcgagcagctgtgggtggacgtgctgcgcgcgtccatggcgcacgggctcacgccggtgtcgtggacagactacctccacctcaccgtcggcggcacgctgtccaacgccggcatcagcggccaggccttccgccatggcccccagatttccaacgtgctagagctcgacgtcatcaccggtgtcggggagatggtgacgtgctcgaaggagaaggcgccggacctgttcgacgcggtgctgggcgggctggggcagttcggcgtcatcacgcgggcgcgcatcccgctcgcgccggcgccggcgagggcgcggtgggtgcggttcgtgtacacgacggcggcggcgatgacggccgaccaggagcgcctcatcgccgtcgatcgcgccggcggcgccggcgcggtgggcgggctgatggactacgtcgagggctcggtccacctgaaccagggcctggtcgagacctggcgcacgcagccgcagccgccttcgccgtcctcctcctcctcctcatccttcttctccgacgccgacgaggcccgcgtcgccgcgctcgccaaggaggccggcggcgtgctgtatttcctcgagggcgccatctacttcggcggcgccgccgggccgtccgccgccgacgttgacaagaggatggatgtgctgcgtcgcgagctgcggcacgagcgcgggttcgtgttcgcgcaggacgtggcgtacgccgggttcctggaccgcgtccacgacggcgagctcaagctccgcgccgcggggctctgggacgtgccgcacccatggctgaacctgttcctcccccgctccggcgtcctcgccttcgccgacggcgtcttccacggcatcctcagccgcacccccgccatgggccccgtcctcatctaccccatgaaccgcaacaagtgggacagtaacatgtcggcagtgatcaccgacgacgacggtgacgaggtgttctacacggtggggatcctgcggtcggcggcggcggccggcgacgtggggaggctggaggagcagaacgacgagatcttgggtttctgcgaggtggccgggatagcctacaagcagtacctgccttactacggcagccaggcagagtggcagaagcggcacttcggtgccaatctctggccaagattcgtgcagcggaagagcaagtatgatccaaaggccatcctgtcccgtggccaggggattttcacgtcaccactcgcatga</cdnaseq>|
AA = <aaseq>MKQEQVRMAVLLMLNCFVKATAPPPWPPSASSASFLDDLGDLGI
APLIRADEAGTARASADFGNLSVAGVGAPRLAAAAAVLYPSRPADIAALLRASCARPA
PFAVSARGCGHSVHGQASAPDGVVVDMASLGRLQGGGARRLAVSVEGRYVDAGGEQLW
VDVLRASMAHGLTPVSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVLELDVITGVG
EMVTCSKEKAPDLFDAVLGGLGQFGVITRARIPLAPAPARARWVRFVYTTAAAMTADQ
ERLIAVDRAGGAGAVGGLMDYVEGSVHLNQGLVETWRTQPQPPSPSSSSSSSFFSDAD
EARVAALAKEAGGVLYFLEGAIYFGGAAGPSAADVDKRMDVLRRELRHERGFVFAQDV
AYAGFLDRVHDGELKLRAAGLWDVPHPWLNLFLPRSGVLAFADGVFHGILSRTPAMGP
VLIYPMNRNKWDSNMSAVITDDDGDEVFYTVGILRSAAAAGDVGRLEEQNDEILGFCE
VAGIAYKQYLPYYGSQAEWQKRHFGANLWPRFVQRKSKYDPKAILSRGQGIFTSPLA</aaseq>|
DNA = <dnaseqindica>1..625#717..1192#ATGGCAGTGCTCCTCATGCTCAACTGCTTCGTCAAGGCCACGGCGCCGCCGCCATGGCCGCCGTCGGCTT
CGTCCGCCTCCTTCCTCGACGACCTCGGCGACCTCGGCATCGCGCCGCTCATCCGCGCCGACGAGGCGGG
CACCGCGCGCGCCTCCGCCGACTTTGGCAACCTCTCCGTCGCCGGCGTCGGGGCGCCTCGGCTCGCCGCC
GCCGCCGCCGTGCTCTACCCGTCGCGCCCCGCCGACATCGCCGCGCTGCTGCGCGCGTCGTGCGCACGCC
CGGCGCCGTTCGCGGTGTCCGCGCGGGGGTGTGGCCACTCGGTGCACGGCCAGGCCTCCGCGCCCGACGG
CGTCGTCGTCGACATGGCGTCGCTCGGCCGCCTGCAGGGCGGCGGCGCGCGGCGCCTCGCCGTGTCAGTG
GAGGGGCGGTACGTCGACGCCGGCGGCGAGCAGCTGTGGGTGGACGTGCTGCGCGCGTCCATGGCGCACG
GGCTCACGCCGGTGTCGTGGACAGACTACCTCCACCTCACCGTCGGCGGCACGCTGTCCAACGCCGGCAT
CAGCGGCCAGGCCTTCCGCCATGGCCCCCAGATTTCCAACGTGCTAGAGCTCGACGTCATCACCGGTACG
TAGATCCATCACATCTACTAAGACACGCGCCGCCATGATCGAGGTAATTAAGGTATAGGTGTTTTGACGT
ATACATGTATCTGCAGGTGTCGGGGAGATGGTGACGTGCTCGAAGGAGAAGGCGCCGGACCTGTTCGACG
CGGTGCTGGGCGGGCTGGGGCAGTTCGGCGTCATCACGCGGGCGCGCATCCCGCTCGCGCCGGCGCCGGC
GAGGGCGCGGTGGGTGCGGTTCGTGTACACGACGGCGGCGGCGATGACGGCCGACCAGGAGCGCCTCATC
GCCGTCGATCGCGCCGGCGGCGCCGGCGCGGTGGGCGGGCTGATGGACTACGTCGAGGGCTCGGTCCACC
TGAACCAGGGCCTGGTCGAGACCTGGCGCACGCAGCCGCAGCCGCCTTCGCCGTCCTCCTCCTCCTCCTC
ATCCTTCTTCTCCGACGCCGACGAGGCCCGCGTCGCCGCGCTCGCCAAGGAGGCCGGCGGCGTGCTGTAT
TTCCTCGAGGGCGCCATCTACTTCGGCGGCGCCGCCGGGCCGTCCGCCGCCGACGTTGACAAGGTATACT
AG
</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NC_008394.3?report=fasta&from=5273310&to=5274501&strand=true]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

File:Fggg.png

2014-05-23T10:22:16Z

潘文波:

File:搜狗截图14年05月23日1804 1.png

2014-05-23T10:16:09Z

潘文波:

File:Eeee.jpg

2014-05-23T10:06:00Z

潘文波:

File:Dfghjj.png

2014-05-23T10:05:00Z

潘文波:

Os01g0197700

2014-05-23T09:47:28Z

潘文波: Created page with "==Structured Information== {{JaponicaGene| GeneName = Os01g0197700| Description = OsCKX2，cytokinin oxidase/dehydrogenase| Version =AB205193.1 GI:71609872.(gene version=Gene..."

==Structured Information==
{{JaponicaGene|
GeneName = Os01g0197700|
Description = OsCKX2，cytokinin oxidase/dehydrogenase|
Version =AB205193.1 GI:71609872.(gene version=Gene ID: 4327334)|
Length = 1698 bp|
Definition = Oryza sativa Japonica Group OsCKX2 mRNA for cytokinin oxidase/dehydrogenase, complete cds.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:5273310..5274501|
CDS = 1..625,717..1192|
GCID = <gbrowseImage1>
name=NC_008394:5273310..5274501
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:5273310..5274501
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgaagcaagagcaggtcaggatggcagtgctcctcatgctcaactgcttcgtcaaggccacggcgccgccgccatggccgccgtcggcttcgtccgcctccttcctcgacgacctcggcgacctcggcatcgcgccgctcatccgcgccgacgaggcgggcaccgcgcgcgcctccgccgactttggcaacctctccgtcgccggcgtcggggcgcctcggctcgccgccgccgccgccgtgctctacccgtcgcgccccgccgacatcgccgcgctgctgcgcgcgtcgtgcgcacgcccggcgccgttcgcggtgtccgcgcgggggtgtggccactcggtgcacggccaggcctccgcgcccgacggcgtcgtcgtcgacatggcgtcgctcggccgcctgcagggcggcggcgcgcggcgcctcgccgtgtcagtggaggggcggtacgtcgacgccggcggcgagcagctgtgggtggacgtgctgcgcgcgtccatggcgcacgggctcacgccggtgtcgtggacagactacctccacctcaccgtcggcggcacgctgtccaacgccggcatcagcggccaggccttccgccatggcccccagatttccaacgtgctagagctcgacgtcatcaccggtgtcggggagatggtgacgtgctcgaaggagaaggcgccggacctgttcgacgcggtgctgggcgggctggggcagttcggcgtcatcacgcgggcgcgcatcccgctcgcgccggcgccggcgagggcgcggtgggtgcggttcgtgtacacgacggcggcggcgatgacggccgaccaggagcgcctcatcgccgtcgatcgcgccggcggcgccggcgcggtgggcgggctgatggactacgtcgagggctcggtccacctgaaccagggcctggtcgagacctggcgcacgcagccgcagccgccttcgccgtcctcctcctcctcctcatccttcttctccgacgccgacgaggcccgcgtcgccgcgctcgccaaggaggccggcggcgtgctgtatttcctcgagggcgccatctacttcggcggcgccgccgggccgtccgccgccgacgttgacaagaggatggatgtgctgcgtcgcgagctgcggcacgagcgcgggttcgtgttcgcgcaggacgtggcgtacgccgggttcctggaccgcgtccacgacggcgagctcaagctccgcgccgcggggctctgggacgtgccgcacccatggctgaacctgttcctcccccgctccggcgtcctcgccttcgccgacggcgtcttccacggcatcctcagccgcacccccgccatgggccccgtcctcatctaccccatgaaccgcaacaagtgggacagtaacatgtcggcagtgatcaccgacgacgacggtgacgaggtgttctacacggtggggatcctgcggtcggcggcggcggccggcgacgtggggaggctggaggagcagaacgacgagatcttgggtttctgcgaggtggccgggatagcctacaagcagtacctgccttactacggcagccaggcagagtggcagaagcggcacttcggtgccaatctctggccaagattcgtgcagcggaagagcaagtatgatccaaaggccatcctgtcccgtggccaggggattttcacgtcaccactcgcatga</cdnaseq>|
AA = <aaseq>MKQEQVRMAVLLMLNCFVKATAPPPWPPSASSASFLDDLGDLGI
APLIRADEAGTARASADFGNLSVAGVGAPRLAAAAAVLYPSRPADIAALLRASCARPA
PFAVSARGCGHSVHGQASAPDGVVVDMASLGRLQGGGARRLAVSVEGRYVDAGGEQLW
VDVLRASMAHGLTPVSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVLELDVITGVG
EMVTCSKEKAPDLFDAVLGGLGQFGVITRARIPLAPAPARARWVRFVYTTAAAMTADQ
ERLIAVDRAGGAGAVGGLMDYVEGSVHLNQGLVETWRTQPQPPSPSSSSSSSFFSDAD
EARVAALAKEAGGVLYFLEGAIYFGGAAGPSAADVDKRMDVLRRELRHERGFVFAQDV
AYAGFLDRVHDGELKLRAAGLWDVPHPWLNLFLPRSGVLAFADGVFHGILSRTPAMGP
VLIYPMNRNKWDSNMSAVITDDDGDEVFYTVGILRSAAAAGDVGRLEEQNDEILGFCE
VAGIAYKQYLPYYGSQAEWQKRHFGANLWPRFVQRKSKYDPKAILSRGQGIFTSPLA</aaseq>|
DNA = <dnaseqindica>1..625#717..1192#ATGGCAGTGCTCCTCATGCTCAACTGCTTCGTCAAGGCCACGGCGCCGCCGCCATGGCCGCCGTCGGCTT
CGTCCGCCTCCTTCCTCGACGACCTCGGCGACCTCGGCATCGCGCCGCTCATCCGCGCCGACGAGGCGGG
CACCGCGCGCGCCTCCGCCGACTTTGGCAACCTCTCCGTCGCCGGCGTCGGGGCGCCTCGGCTCGCCGCC
GCCGCCGCCGTGCTCTACCCGTCGCGCCCCGCCGACATCGCCGCGCTGCTGCGCGCGTCGTGCGCACGCC
CGGCGCCGTTCGCGGTGTCCGCGCGGGGGTGTGGCCACTCGGTGCACGGCCAGGCCTCCGCGCCCGACGG
CGTCGTCGTCGACATGGCGTCGCTCGGCCGCCTGCAGGGCGGCGGCGCGCGGCGCCTCGCCGTGTCAGTG
GAGGGGCGGTACGTCGACGCCGGCGGCGAGCAGCTGTGGGTGGACGTGCTGCGCGCGTCCATGGCGCACG
GGCTCACGCCGGTGTCGTGGACAGACTACCTCCACCTCACCGTCGGCGGCACGCTGTCCAACGCCGGCAT
CAGCGGCCAGGCCTTCCGCCATGGCCCCCAGATTTCCAACGTGCTAGAGCTCGACGTCATCACCGGTACG
TAGATCCATCACATCTACTAAGACACGCGCCGCCATGATCGAGGTAATTAAGGTATAGGTGTTTTGACGT
ATACATGTATCTGCAGGTGTCGGGGAGATGGTGACGTGCTCGAAGGAGAAGGCGCCGGACCTGTTCGACG
CGGTGCTGGGCGGGCTGGGGCAGTTCGGCGTCATCACGCGGGCGCGCATCCCGCTCGCGCCGGCGCCGGC
GAGGGCGCGGTGGGTGCGGTTCGTGTACACGACGGCGGCGGCGATGACGGCCGACCAGGAGCGCCTCATC
GCCGTCGATCGCGCCGGCGGCGCCGGCGCGGTGGGCGGGCTGATGGACTACGTCGAGGGCTCGGTCCACC
TGAACCAGGGCCTGGTCGAGACCTGGCGCACGCAGCCGCAGCCGCCTTCGCCGTCCTCCTCCTCCTCCTC
ATCCTTCTTCTCCGACGCCGACGAGGCCCGCGTCGCCGCGCTCGCCAAGGAGGCCGGCGGCGTGCTGTAT
TTCCTCGAGGGCGCCATCTACTTCGGCGGCGCCGCCGGGCCGTCCGCCGCCGACGTTGACAAGGTATACT
AG
</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NC_008394.3?report=fasta&from=5273310&to=5274501&strand=true]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]