http://192.168.164.12:81/ricewiki/api.php?action=feedcontributions&feedformat=atom&user=BaixueluoRiceWiki - User contributions [en]2026-05-08T17:23:45ZUser contributionsMediaWiki 1.30.0https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175715Os06g05695002014-06-01T14:39:31Z<p>Baixueluo: /* Evolution */</p>
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<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
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===Expression==<br />
[[File:1.png|right|thumb|150px| ''The biosynthetic pathway of GA.(from reference'' <ref name="ref1" />)]]<br />
[[File:2.png|right|thumb|150px| ''GA biosynthesis.(from reference'' <ref name="ref2" />)]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ''ent''-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.The bioactive GAs (GA1and GA4) are synthesized through a series of oxidation reactions of ''ent''-kaurene by two types of oxidases. Both ''ent''-kaurene oxidase and ''ent''-kaurenoic acid oxidase are cytochrome P450 monoo xygenases that success ively convert ''ent''-kaurene to GA12.GA12 is further converted tobioactive GA s by two 2-oxoglutarate-dependent dioxygenases, GA 20-oxidase and GA3-oxidase.GA 2-oxidase is another member of the 2-oxoglutarate-dependent dioxygenase family and is responsible for GA inactivation<ref name="ref1" />.The ring contraction catalyzed by the cytochrome P450 (CYP) ''ent''-kaurenoic acid oxidase (KAO) is then the committed step in GA-specific biosynthesis.OsKO2 exhibits KO activity, OsKOL 4 does not, instead catalyzing C3a hydroxylation of ''ent''-sandaracopimaradiene and ''ent''-cassadiene, as well as ''ent''-kaurene <ref name="ref2" />.<br />
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===Evolution===<br />
[[File:4.png|right|thumb|150px| ''Phenotypic Characterization of the gdd1 Mutant.(from reference'' <ref name="ref4" />)]]<br />
[[File:5.png|right|thumb|150px| ''Map-Based Cloning of GDD1 and Complementation Test.(from reference''<ref name="ref4" />)]]<br />
A rice mutant with greatly decreased height was isolated from a transgene line after ''Agrobacterium tumifaciens''–mediated T-DNA insertion. The mutant, designated ''gibberellin-deficient dwarf1(gdd1)'', retained a stable phenotype and showed segregation of the b-glucuronidase (GUS) gene with the T-DNA insertions until T2 progeny, suggesting that the phenotype was independent of the T-DNA insertion, the mutant with neither the T-DNA insertion nor GUS fragment from the T2 progeny was used for analysis the function of ''GDD1'' .To understand the molecular mechanism responsible for the<br />
gdd1 phenotype, we used a map-based cloning approach to isolate GDD1. All F1 progeny showed a height phenotype similar to that of the wild type. Tests of heterozygotes with F2 progeny yielded a segregation of 401 normal and 144 dwarf plants (x2[3:1] = 0.59 < x20.05= 3.84; P > 0.05), indicating that the dwarf phenotype of the gdd1 mutant is caused by a recessive mutation in a single nuclear gene<ref name="ref4" />.The GA7 gene encodes the enzyme ''ent''-kaurene synthetase A, which catalyzes the conversion of GGPP to CPP. Subcellular localization of the GA1 protein was studied using 35S-labeled GA1 protein and isolated pea chloroplasts. The results showed that the GAl protein is imported into and processed in pea chloroplasts in vitro.DNA sequences of GA7 genomic DNA and cDNA were obtained using the dideoxy method with Sequenase version 2.0 T7 DNA polymerase (U.S.Biochemical Corp.)and both single and double-stranded DNA templates.The 1.4-kb Hindlll DNA in the ga7-9 mutant was amplified by PCR and reamplified by asymmetric PCR, and the single-stranded DNA templates were sequenced directly. The 1.4-kb DNA fragments spanning intron 12 to exon 15 were amplified from genomic DNA isolated from ga7-7 and ga7-4 by PCR. These PCR-amplified DNA products were cloned into the Smal site of the pBluescript SK+ vector, and DNA sequences were obtained by using double-stranded DNA templates isolated from severa1 independent clones<ref name="ref6" />.<br />
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==Labs working on this gene==<br />
Department of Biochemistry, Okayama University of Science, Okayama 700–0005, Japan<br />
National Institute for Basic B iology, O kazaki444–8585, Japan<br />
School of Life Science,Grad uate University for Advanced Studies, O kazaki 444–8585, Japan<br />
Department of Botany, Box 91000, Duke University, Durham, North Carolina 27708-1000<br />
Frontier Research Program, The lnstitute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama 351-01, Japan<br />
Research Center for Molecular and Developmental Biology, Key Laboratory of Photosyn thesis and Environ mental Molecular<br />
Physiology, Institute of Botany , Chinese Academy of Sciences , Beijing 100093, China<br />
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==References==<br />
1、Ken-ichiro Hayashi, Keisuke Horie, Yu ji Hiwatashi et al.（2012）Endogenous Diterpenes Derived from ''ent''-Kaurene, a Common Gibberellin Precursor, Regulate Protonema Diff erentiation of the Moss ''Physcomitrella patens''1[W][OA]. Plant Physiology 153:1085-1097.<br />
<br />
2、Qiang Wang, Matthew L . Hillwig, Yisheng Wu et al.（2012）CYP701A8: A Rice ''ent''-Kaurene Oxidase P aralog Diverted to More Specialized Diterpenoid Metabolism1[W][OA]. Plant Physiology 158:1418-1425.<br />
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3、Shifeng Zhu , Feng Gao, Xue song Cao et al.(2005)The Rice Dwarf Virus P2 Protein Interacts with ''ent''-Kaurene Oxidases in Vivo, Leading to Reduced Biosynthesis of Gibberellins and Rice Dwarf Symptoms1.Plant Physiology 139:1935-1945.<br />
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4、Juan Li,Jiafu Jiang,Qian Qian et al.(2011)Mutation of Rice BC12/GDD1, Which Encodes a Kinesin-Like Protein That Binds to a GA Biosynthesis Gene Promoter, Leads to Dwarfism with Impaired Cell Elongation.The Plant Cell 23:628-640.<br />
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5、Fritz M. Schomburg,Colleen M. Bizzell,Dong Ju Lee et ai.(2003)Overexpression of a Novel Class of Gibberellin 2-Oxidases Decreases Gibberellin Levels and Creates Dwarf Plants.The Plant Cell 15:151-163. <br />
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6、Tai-ping Sun,Yuji Kamiya.(1994)The Arabidopsis GAl Locus Encodes the Cyclase ''ent''-Kaurene Synthetase A of Gibberellin Biosynthesis.The Plant Cell 6:1509-1518.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175714Os06g05695002014-06-01T14:37:37Z<p>Baixueluo: /* References */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
<br />
===Expression==<br />
[[File:1.png|right|thumb|150px| ''The biosynthetic pathway of GA.(from reference'' <ref name="ref1" />)]]<br />
[[File:2.png|right|thumb|150px| ''GA biosynthesis.(from reference'' <ref name="ref2" />)]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ''ent''-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.The bioactive GAs (GA1and GA4) are synthesized through a series of oxidation reactions of ''ent''-kaurene by two types of oxidases. Both ''ent''-kaurene oxidase and ''ent''-kaurenoic acid oxidase are cytochrome P450 monoo xygenases that success ively convert ''ent''-kaurene to GA12.GA12 is further converted tobioactive GA s by two 2-oxoglutarate-dependent dioxygenases, GA 20-oxidase and GA3-oxidase.GA 2-oxidase is another member of the 2-oxoglutarate-dependent dioxygenase family and is responsible for GA inactivation<ref name="ref1" />.The ring contraction catalyzed by the cytochrome P450 (CYP) ''ent''-kaurenoic acid oxidase (KAO) is then the committed step in GA-specific biosynthesis.OsKO2 exhibits KO activity, OsKOL 4 does not, instead catalyzing C3a hydroxylation of ''ent''-sandaracopimaradiene and ''ent''-cassadiene, as well as ''ent''-kaurene <ref name="ref2" />.<br />
<br />
===Evolution===<br />
[[File:4.png|right|thumb|150px| ''Phenotypic Characterization of the gdd1 Mutant.(from reference'' <ref name="ref4" />)]]<br />
[[File:5.png|right|thumb|150px| ''Map-Based Cloning of GDD1 and Complementation Test.(from reference''<ref name="ref4" />)]]<br />
A rice mutant with greatly decreased height was isolated from a transgene line after ''Agrobacterium tumifaciens''–mediated T-DNA insertion. The mutant, designated ''gibberellin-deficient dwarf1(gdd1)'', retained a stable phenotype and showed segregation of the b-glucuronidase (GUS) gene with the T-DNA insertions until T2 progeny, suggesting that the phenotype was independent of the T-DNA insertion, the mutant with neither the T-DNA insertion nor GUS fragment from the T2 progeny was used for analysis the function of ''GDD1'' .To understand the molecular mechanism responsible for the<br />
gdd1 phenotype, we used a map-based cloning approach to isolate GDD1. All F1 progeny showed a height phenotype similar to that of the wild type. Tests of heterozygotes with F2 progeny yielded a segregation of 401 normal and 144 dwarf plants (x2[3:1] = 0.59 < x20.05= 3.84; P > 0.05), indicating that the dwarf phenotype of the gdd1 mutant is caused by a recessive mutation in a single nuclear gene<ref name="ref4" />.The GA7 gene encodes the enzyme ''ent''-kaurene synthetase A, which catalyzes the conversion of GGPP to CPP. Subcellular localization of the GA1 protein was studied using 35S-labeled GA1 protein and isolated pea chloroplasts. The results showed that the GAl protein is imported into and processed in pea chloroplasts in vitro.DNA sequences of GA7 genomic DNA and cDNA were obtained using the dideoxy method with Sequenase version 2.0 T7 DNA polymerase (U.S.Biochemical Corp.)and both single and double-stranded DNA templates.The 1.4-kb Hindlll DNA in the ga7-9 mutant was amplified by PCR and reamplified by asymmetric PCR, and the single-stranded DNA templates were sequenced directly. The 1.4-kb DNA fragments spanning intron 12 to exon 15 were amplified from genomic DNA isolated from ga7-7 and ga7-4 by PCR. These PCR-amplified DNA products were cloned into the Smal site of the pBluescript SK+ vector, and DNA sequences were obtained by using double-stranded DNA templates isolated from severa1 independent clones<ref name="ref6" />. <br />
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You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Department of Biochemistry, Okayama University of Science, Okayama 700–0005, Japan<br />
National Institute for Basic B iology, O kazaki444–8585, Japan<br />
School of Life Science,Grad uate University for Advanced Studies, O kazaki 444–8585, Japan<br />
Department of Botany, Box 91000, Duke University, Durham, North Carolina 27708-1000<br />
Frontier Research Program, The lnstitute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama 351-01, Japan<br />
Research Center for Molecular and Developmental Biology, Key Laboratory of Photosyn thesis and Environ mental Molecular<br />
Physiology, Institute of Botany , Chinese Academy of Sciences , Beijing 100093, China<br />
<br />
==References==<br />
1、Ken-ichiro Hayashi, Keisuke Horie, Yu ji Hiwatashi et al.（2012）Endogenous Diterpenes Derived from ''ent''-Kaurene, a Common Gibberellin Precursor, Regulate Protonema Diff erentiation of the Moss ''Physcomitrella patens''1[W][OA]. Plant Physiology 153:1085-1097.<br />
<br />
2、Qiang Wang, Matthew L . Hillwig, Yisheng Wu et al.（2012）CYP701A8: A Rice ''ent''-Kaurene Oxidase P aralog Diverted to More Specialized Diterpenoid Metabolism1[W][OA]. Plant Physiology 158:1418-1425.<br />
<br />
3、Shifeng Zhu , Feng Gao, Xue song Cao et al.(2005)The Rice Dwarf Virus P2 Protein Interacts with ''ent''-Kaurene Oxidases in Vivo, Leading to Reduced Biosynthesis of Gibberellins and Rice Dwarf Symptoms1.Plant Physiology 139:1935-1945.<br />
<br />
4、Juan Li,Jiafu Jiang,Qian Qian et al.(2011)Mutation of Rice BC12/GDD1, Which Encodes a Kinesin-Like Protein That Binds to a GA Biosynthesis Gene Promoter, Leads to Dwarfism with Impaired Cell Elongation.The Plant Cell 23:628-640.<br />
<br />
5、Fritz M. Schomburg,Colleen M. Bizzell,Dong Ju Lee et ai.(2003)Overexpression of a Novel Class of Gibberellin 2-Oxidases Decreases Gibberellin Levels and Creates Dwarf Plants.The Plant Cell 15:151-163. <br />
<br />
6、Tai-ping Sun,Yuji Kamiya.(1994)The Arabidopsis GAl Locus Encodes the Cyclase ''ent''-Kaurene Synthetase A of Gibberellin Biosynthesis.The Plant Cell 6:1509-1518.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175713Os06g05695002014-06-01T14:35:01Z<p>Baixueluo: /* References */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
<br />
===Expression==<br />
[[File:1.png|right|thumb|150px| ''The biosynthetic pathway of GA.(from reference'' <ref name="ref1" />)]]<br />
[[File:2.png|right|thumb|150px| ''GA biosynthesis.(from reference'' <ref name="ref2" />)]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ''ent''-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.The bioactive GAs (GA1and GA4) are synthesized through a series of oxidation reactions of ''ent''-kaurene by two types of oxidases. Both ''ent''-kaurene oxidase and ''ent''-kaurenoic acid oxidase are cytochrome P450 monoo xygenases that success ively convert ''ent''-kaurene to GA12.GA12 is further converted tobioactive GA s by two 2-oxoglutarate-dependent dioxygenases, GA 20-oxidase and GA3-oxidase.GA 2-oxidase is another member of the 2-oxoglutarate-dependent dioxygenase family and is responsible for GA inactivation<ref name="ref1" />.The ring contraction catalyzed by the cytochrome P450 (CYP) ''ent''-kaurenoic acid oxidase (KAO) is then the committed step in GA-specific biosynthesis.OsKO2 exhibits KO activity, OsKOL 4 does not, instead catalyzing C3a hydroxylation of ''ent''-sandaracopimaradiene and ''ent''-cassadiene, as well as ''ent''-kaurene <ref name="ref2" />.<br />
<br />
===Evolution===<br />
[[File:4.png|right|thumb|150px| ''Phenotypic Characterization of the gdd1 Mutant.(from reference'' <ref name="ref4" />)]]<br />
[[File:5.png|right|thumb|150px| ''Map-Based Cloning of GDD1 and Complementation Test.(from reference''<ref name="ref4" />)]]<br />
A rice mutant with greatly decreased height was isolated from a transgene line after ''Agrobacterium tumifaciens''–mediated T-DNA insertion. The mutant, designated ''gibberellin-deficient dwarf1(gdd1)'', retained a stable phenotype and showed segregation of the b-glucuronidase (GUS) gene with the T-DNA insertions until T2 progeny, suggesting that the phenotype was independent of the T-DNA insertion, the mutant with neither the T-DNA insertion nor GUS fragment from the T2 progeny was used for analysis the function of ''GDD1'' .To understand the molecular mechanism responsible for the<br />
gdd1 phenotype, we used a map-based cloning approach to isolate GDD1. All F1 progeny showed a height phenotype similar to that of the wild type. Tests of heterozygotes with F2 progeny yielded a segregation of 401 normal and 144 dwarf plants (x2[3:1] = 0.59 < x20.05= 3.84; P > 0.05), indicating that the dwarf phenotype of the gdd1 mutant is caused by a recessive mutation in a single nuclear gene<ref name="ref4" />.The GA7 gene encodes the enzyme ''ent''-kaurene synthetase A, which catalyzes the conversion of GGPP to CPP. Subcellular localization of the GA1 protein was studied using 35S-labeled GA1 protein and isolated pea chloroplasts. The results showed that the GAl protein is imported into and processed in pea chloroplasts in vitro.DNA sequences of GA7 genomic DNA and cDNA were obtained using the dideoxy method with Sequenase version 2.0 T7 DNA polymerase (U.S.Biochemical Corp.)and both single and double-stranded DNA templates.The 1.4-kb Hindlll DNA in the ga7-9 mutant was amplified by PCR and reamplified by asymmetric PCR, and the single-stranded DNA templates were sequenced directly. The 1.4-kb DNA fragments spanning intron 12 to exon 15 were amplified from genomic DNA isolated from ga7-7 and ga7-4 by PCR. These PCR-amplified DNA products were cloned into the Smal site of the pBluescript SK+ vector, and DNA sequences were obtained by using double-stranded DNA templates isolated from severa1 independent clones<ref name="ref6" />. <br />
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You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Department of Biochemistry, Okayama University of Science, Okayama 700–0005, Japan<br />
National Institute for Basic B iology, O kazaki444–8585, Japan<br />
School of Life Science,Grad uate University for Advanced Studies, O kazaki 444–8585, Japan<br />
Department of Botany, Box 91000, Duke University, Durham, North Carolina 27708-1000<br />
Frontier Research Program, The lnstitute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama 351-01, Japan<br />
Research Center for Molecular and Developmental Biology, Key Laboratory of Photosyn thesis and Environ mental Molecular<br />
Physiology, Institute of Botany , Chinese Academy of Sciences , Beijing 100093, China<br />
<br />
==References==<br />
1、Ken-ichiro Hayashi, Keisuke Horie, Yu ji Hiwatashi et al.（2012）Endogenous Diterpenes Derived froment-Kaurene, a Common Gibberellin Precursor, Regulate Protonema Diff erentiation of the Moss ''Physcomitrella patens''1[W][OA]. Plant Physiology 153:1085-1097.<br />
<br />
2、Qiang Wang, Matthew L . Hillwig, Yisheng Wu et al.（2012）CYP701A8: A Rice ent-Kaurene Oxidase P aralog Diverted to More Specialized Diterpenoid Metabolism1[W][OA]. Plant Physiology 158:1418-1425.<br />
<br />
3、Shifeng Zhu , Feng Gao, Xue song Cao et al.(2005)The Rice Dwarf Virus P2 Protein Interacts with ''ent''-Kaurene Oxidases in Vivo, Leading to Reduced Biosynthesis of Gibberellins and Rice Dwarf Symptoms1.Plant Physiology 139:1935-1945.<br />
<br />
4、Juan Li,Jiafu Jiang,Qian Qian et al.(2011)Mutation of Rice BC12/GDD1, Which Encodes a Kinesin-Like Protein That Binds to a GA Biosynthesis Gene Promoter, Leads to Dwarfism with Impaired Cell Elongation.The Plant Cell 23:628-640.<br />
<br />
5、Fritz M. Schomburg,Colleen M. Bizzell,Dong Ju Lee et ai.(2003)Overexpression of a Novel Class of Gibberellin 2-Oxidases Decreases Gibberellin Levels and Creates Dwarf Plants.The Plant Cell 15:151-163. <br />
<br />
6、Tai-ping Sun,Yuji Kamiya.(1994)The Arabidopsis GAl Locus Encodes the Cyclase ''ent''-Kaurene Synthetase A of Gibberellin Biosynthesis.The Plant Cell 6:1509-1518.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175710Os06g05695002014-06-01T14:23:52Z<p>Baixueluo: /* References */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
<br />
===Expression==<br />
[[File:1.png|right|thumb|150px| ''The biosynthetic pathway of GA.(from reference'' <ref name="ref1" />)]]<br />
[[File:2.png|right|thumb|150px| ''GA biosynthesis.(from reference'' <ref name="ref2" />)]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ''ent''-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.The bioactive GAs (GA1and GA4) are synthesized through a series of oxidation reactions of ''ent''-kaurene by two types of oxidases. Both ''ent''-kaurene oxidase and ''ent''-kaurenoic acid oxidase are cytochrome P450 monoo xygenases that success ively convert ''ent''-kaurene to GA12.GA12 is further converted tobioactive GA s by two 2-oxoglutarate-dependent dioxygenases, GA 20-oxidase and GA3-oxidase.GA 2-oxidase is another member of the 2-oxoglutarate-dependent dioxygenase family and is responsible for GA inactivation<ref name="ref1" />.The ring contraction catalyzed by the cytochrome P450 (CYP) ''ent''-kaurenoic acid oxidase (KAO) is then the committed step in GA-specific biosynthesis.OsKO2 exhibits KO activity, OsKOL 4 does not, instead catalyzing C3a hydroxylation of ''ent''-sandaracopimaradiene and ''ent''-cassadiene, as well as ''ent''-kaurene <ref name="ref2" />.<br />
<br />
===Evolution===<br />
[[File:4.png|right|thumb|150px| ''Phenotypic Characterization of the gdd1 Mutant.(from reference'' <ref name="ref4" />)]]<br />
[[File:5.png|right|thumb|150px| ''Map-Based Cloning of GDD1 and Complementation Test.(from reference''<ref name="ref4" />)]]<br />
A rice mutant with greatly decreased height was isolated from a transgene line after ''Agrobacterium tumifaciens''–mediated T-DNA insertion. The mutant, designated ''gibberellin-deficient dwarf1(gdd1)'', retained a stable phenotype and showed segregation of the b-glucuronidase (GUS) gene with the T-DNA insertions until T2 progeny, suggesting that the phenotype was independent of the T-DNA insertion, the mutant with neither the T-DNA insertion nor GUS fragment from the T2 progeny was used for analysis the function of ''GDD1'' .To understand the molecular mechanism responsible for the<br />
gdd1 phenotype, we used a map-based cloning approach to isolate GDD1. All F1 progeny showed a height phenotype similar to that of the wild type. Tests of heterozygotes with F2 progeny yielded a segregation of 401 normal and 144 dwarf plants (x2[3:1] = 0.59 < x20.05= 3.84; P > 0.05), indicating that the dwarf phenotype of the gdd1 mutant is caused by a recessive mutation in a single nuclear gene<ref name="ref4" />.The GA7 gene encodes the enzyme ''ent''-kaurene synthetase A, which catalyzes the conversion of GGPP to CPP. Subcellular localization of the GA1 protein was studied using 35S-labeled GA1 protein and isolated pea chloroplasts. The results showed that the GAl protein is imported into and processed in pea chloroplasts in vitro.DNA sequences of GA7 genomic DNA and cDNA were obtained using the dideoxy method with Sequenase version 2.0 T7 DNA polymerase (U.S.Biochemical Corp.)and both single and double-stranded DNA templates.The 1.4-kb Hindlll DNA in the ga7-9 mutant was amplified by PCR and reamplified by asymmetric PCR, and the single-stranded DNA templates were sequenced directly. The 1.4-kb DNA fragments spanning intron 12 to exon 15 were amplified from genomic DNA isolated from ga7-7 and ga7-4 by PCR. These PCR-amplified DNA products were cloned into the Smal site of the pBluescript SK+ vector, and DNA sequences were obtained by using double-stranded DNA templates isolated from severa1 independent clones<ref name="ref6" />. <br />
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You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Department of Biochemistry, Okayama University of Science, Okayama 700–0005, Japan<br />
National Institute for Basic B iology, O kazaki444–8585, Japan<br />
School of Life Science,Grad uate University for Advanced Studies, O kazaki 444–8585, Japan<br />
Department of Botany, Box 91000, Duke University, Durham, North Carolina 27708-1000<br />
Frontier Research Program, The lnstitute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama 351-01, Japan<br />
Research Center for Molecular and Developmental Biology, Key Laboratory of Photosyn thesis and Environ mental Molecular<br />
Physiology, Institute of Botany , Chinese Academy of Sciences , Beijing 100093, China<br />
<br />
==References==<br />
1、Ken-ichiro Hayashi, Keisuke Horie, Yu ji Hiwatashi et al.（2012）Endogenous Diterpenes Derived froment-Kaurene, a Common Gibberellin Precursor, Regulate Protonema Diff erentiation of the Moss ''Physcomitrella patens''1[W][OA]. Plant Physiology 153:1085-1097.<br />
<br />
2、Qiang Wang, Matthew L . Hillwig, Yisheng Wu et al.（2012）CYP701A8: A Rice ent-Kaurene Oxidase P aralog Diverted to More Specialized Diterpenoid Metabolism1[W][OA]. Plant Physiology 158:1418-1425.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175707Os06g05695002014-06-01T14:22:16Z<p>Baixueluo: /* =Expression */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
<br />
===Expression==<br />
[[File:1.png|right|thumb|150px| ''The biosynthetic pathway of GA.(from reference'' <ref name="ref1" />)]]<br />
[[File:2.png|right|thumb|150px| ''GA biosynthesis.(from reference'' <ref name="ref2" />)]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ''ent''-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.The bioactive GAs (GA1and GA4) are synthesized through a series of oxidation reactions of ''ent''-kaurene by two types of oxidases. Both ''ent''-kaurene oxidase and ''ent''-kaurenoic acid oxidase are cytochrome P450 monoo xygenases that success ively convert ''ent''-kaurene to GA12.GA12 is further converted tobioactive GA s by two 2-oxoglutarate-dependent dioxygenases, GA 20-oxidase and GA3-oxidase.GA 2-oxidase is another member of the 2-oxoglutarate-dependent dioxygenase family and is responsible for GA inactivation<ref name="ref1" />.The ring contraction catalyzed by the cytochrome P450 (CYP) ''ent''-kaurenoic acid oxidase (KAO) is then the committed step in GA-specific biosynthesis.OsKO2 exhibits KO activity, OsKOL 4 does not, instead catalyzing C3a hydroxylation of ''ent''-sandaracopimaradiene and ''ent''-cassadiene, as well as ''ent''-kaurene <ref name="ref2" />.<br />
<br />
===Evolution===<br />
[[File:4.png|right|thumb|150px| ''Phenotypic Characterization of the gdd1 Mutant.(from reference'' <ref name="ref4" />)]]<br />
[[File:5.png|right|thumb|150px| ''Map-Based Cloning of GDD1 and Complementation Test.(from reference''<ref name="ref4" />)]]<br />
A rice mutant with greatly decreased height was isolated from a transgene line after ''Agrobacterium tumifaciens''–mediated T-DNA insertion. The mutant, designated ''gibberellin-deficient dwarf1(gdd1)'', retained a stable phenotype and showed segregation of the b-glucuronidase (GUS) gene with the T-DNA insertions until T2 progeny, suggesting that the phenotype was independent of the T-DNA insertion, the mutant with neither the T-DNA insertion nor GUS fragment from the T2 progeny was used for analysis the function of ''GDD1'' .To understand the molecular mechanism responsible for the<br />
gdd1 phenotype, we used a map-based cloning approach to isolate GDD1. All F1 progeny showed a height phenotype similar to that of the wild type. Tests of heterozygotes with F2 progeny yielded a segregation of 401 normal and 144 dwarf plants (x2[3:1] = 0.59 < x20.05= 3.84; P > 0.05), indicating that the dwarf phenotype of the gdd1 mutant is caused by a recessive mutation in a single nuclear gene<ref name="ref4" />.The GA7 gene encodes the enzyme ''ent''-kaurene synthetase A, which catalyzes the conversion of GGPP to CPP. Subcellular localization of the GA1 protein was studied using 35S-labeled GA1 protein and isolated pea chloroplasts. The results showed that the GAl protein is imported into and processed in pea chloroplasts in vitro.DNA sequences of GA7 genomic DNA and cDNA were obtained using the dideoxy method with Sequenase version 2.0 T7 DNA polymerase (U.S.Biochemical Corp.)and both single and double-stranded DNA templates.The 1.4-kb Hindlll DNA in the ga7-9 mutant was amplified by PCR and reamplified by asymmetric PCR, and the single-stranded DNA templates were sequenced directly. The 1.4-kb DNA fragments spanning intron 12 to exon 15 were amplified from genomic DNA isolated from ga7-7 and ga7-4 by PCR. These PCR-amplified DNA products were cloned into the Smal site of the pBluescript SK+ vector, and DNA sequences were obtained by using double-stranded DNA templates isolated from severa1 independent clones<ref name="ref6" />. <br />
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You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
Department of Biochemistry, Okayama University of Science, Okayama 700–0005, Japan<br />
National Institute for Basic B iology, O kazaki444–8585, Japan<br />
School of Life Science,Grad uate University for Advanced Studies, O kazaki 444–8585, Japan<br />
Department of Botany, Box 91000, Duke University, Durham, North Carolina 27708-1000<br />
Frontier Research Program, The lnstitute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama 351-01, Japan<br />
Research Center for Molecular and Developmental Biology, Key Laboratory of Photosyn thesis and Environ mental Molecular<br />
Physiology, Institute of Botany , Chinese Academy of Sciences , Beijing 100093, China<br />
<br />
==References==<br />
1、Ken-ichiro Hayashi, Keisuke Horie, Yu ji Hiwatashi et al.（2012）Endogenous Diterpenes Derived froment-Kaurene, a Common Gibberellin Precursor, Regulate Protonema Diff erentiation of the Moss ''Physcomitrella patens'' <ref name="ref1[W][OA]" />. Plant Physiology 153:1085-1097.<br />
2、Qiang Wang, Matthew L . Hillwig, Yisheng Wu et al.（2012）CYP701A8: A Rice ent-Kaurene Oxidase P aralog Diverted to More Specialized Diterpenoid Metabolism1<ref name="ref1[W][OA]" />. Plant Physiology 158:1418-1425.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175706Os06g05695002014-06-01T14:21:12Z<p>Baixueluo: /* =Expression */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
<br />
===Expression==<br />
[[File:1.png|right|thumb|150px| The biosynthetic pathway of GA.(from reference <ref name="ref1" />)]]<br />
[[File:2.png|right|thumb|150px| GA biosynthesis.(from reference <ref name="ref2" />)]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ''ent''-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.The bioactive GAs (GA1and GA4) are synthesized through a series of oxidation reactions of ''ent''-kaurene by two types of oxidases. Both ''ent''-kaurene oxidase and ''ent''-kaurenoic acid oxidase are cytochrome P450 monoo xygenases that success ively convert ''ent''-kaurene to GA12.GA12 is further converted tobioactive GA s by two 2-oxoglutarate-dependent dioxygenases, GA 20-oxidase and GA3-oxidase.GA 2-oxidase is another member of the 2-oxoglutarate-dependent dioxygenase family and is responsible for GA inactivation<ref name="ref1" />.The ring contraction catalyzed by the cytochrome P450 (CYP) ''ent''-kaurenoic acid oxidase (KAO) is then the committed step in GA-specific biosynthesis.OsKO2 exhibits KO activity, OsKOL 4 does not, instead catalyzing C3a hydroxylation of ''ent''-sandaracopimaradiene and ''ent''-cassadiene, as well as ''ent''-kaurene <ref name="ref2" />.<br />
<br />
===Evolution===<br />
[[File:4.png|right|thumb|150px| ''Phenotypic Characterization of the gdd1 Mutant.(from reference'' <ref name="ref4" />)]]<br />
[[File:5.png|right|thumb|150px| ''Map-Based Cloning of GDD1 and Complementation Test.(from reference'' <ref name="ref4" />)]]<br />
A rice mutant with greatly decreased height was isolated from a transgene line after ''Agrobacterium tumifaciens''–mediated T-DNA insertion. The mutant, designated ''gibberellin-deficient dwarf1(gdd1)'', retained a stable phenotype and showed segregation of the b-glucuronidase (GUS) gene with the T-DNA insertions until T2 progeny, suggesting that the phenotype was independent of the T-DNA insertion, the mutant with neither the T-DNA insertion nor GUS fragment from the T2 progeny was used for analysis the function of ''GDD1'' .To understand the molecular mechanism responsible for the<br />
gdd1 phenotype, we used a map-based cloning approach to isolate GDD1. All F1 progeny showed a height phenotype similar to that of the wild type. Tests of heterozygotes with F2 progeny yielded a segregation of 401 normal and 144 dwarf plants (x2[3:1] = 0.59 < x20.05= 3.84; P > 0.05), indicating that the dwarf phenotype of the gdd1 mutant is caused by a recessive mutation in a single nuclear gene<ref name="ref4" />.The GA7 gene encodes the enzyme ''ent''-kaurene synthetase A, which catalyzes the conversion of GGPP to CPP. Subcellular localization of the GA1 protein was studied using 35S-labeled GA1 protein and isolated pea chloroplasts. The results showed that the GAl protein is imported into and processed in pea chloroplasts in vitro.DNA sequences of GA7 genomic DNA and cDNA were obtained using the dideoxy method with Sequenase version 2.0 T7 DNA polymerase (U.S.Biochemical Corp.)and both single and double-stranded DNA templates.The 1.4-kb Hindlll DNA in the ga7-9 mutant was amplified by PCR and reamplified by asymmetric PCR, and the single-stranded DNA templates were sequenced directly. The 1.4-kb DNA fragments spanning intron 12 to exon 15 were amplified from genomic DNA isolated from ga7-7 and ga7-4 by PCR. These PCR-amplified DNA products were cloned into the Smal site of the pBluescript SK+ vector, and DNA sequences were obtained by using double-stranded DNA templates isolated from severa1 independent clones<ref name="ref6" />. <br />
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You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Department of Biochemistry, Okayama University of Science, Okayama 700–0005, Japan<br />
National Institute for Basic B iology, O kazaki444–8585, Japan<br />
School of Life Science,Grad uate University for Advanced Studies, O kazaki 444–8585, Japan<br />
Department of Botany, Box 91000, Duke University, Durham, North Carolina 27708-1000<br />
Frontier Research Program, The lnstitute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama 351-01, Japan<br />
Research Center for Molecular and Developmental Biology, Key Laboratory of Photosyn thesis and Environ mental Molecular<br />
Physiology, Institute of Botany , Chinese Academy of Sciences , Beijing 100093, China<br />
<br />
==References==<br />
1、Ken-ichiro Hayashi, Keisuke Horie, Yu ji Hiwatashi et al.（2012）Endogenous Diterpenes Derived froment-Kaurene, a Common Gibberellin Precursor, Regulate Protonema Diff erentiation of the Moss ''Physcomitrella patens'' <ref name="ref1[W][OA]" />. Plant Physiology 153:1085-1097.<br />
2、Qiang Wang, Matthew L . Hillwig, Yisheng Wu et al.（2012）CYP701A8: A Rice ent-Kaurene Oxidase P aralog Diverted to More Specialized Diterpenoid Metabolism1<ref name="ref1[W][OA]" />. Plant Physiology 158:1418-1425.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175705Os06g05695002014-06-01T14:19:53Z<p>Baixueluo: /* References */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
<br />
===Expression==<br />
[[File:1.png|right|thumb|150px| The biosynthetic pathway of GA.(from reference <ref name="ref1" />)]]<br />
[[File:2.png|right|thumb|150px| GA biosynthesis.(from reference <ref name="ref2" />)]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ''ent''-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.The bioactive GAs (GA1and GA4) are synthesized through a series of oxidation reactions of ''ent''-kaurene by two types of oxidases. Both ''ent''-kaurene oxidase and ''ent''-kaurenoic acid oxidase are cytochrome P450 monoo xygenases that success ively convert ''ent''-kaurene to GA12.GA12 is further converted tobioactive GA s by two 2-oxoglutarate-dependent dioxygenases, GA 20-oxidase and GA3-oxidase.GA 2-oxidase is another member of the 2-oxoglutarate-dependent dioxygenase family and is responsible for GA inactivation<ref name="ref1" />.The ring contraction catalyzed by the cytochrome P450 (CYP) ''ent''-kaurenoic acid oxidase (KAO) is then the committed step in GA-specific biosynthesis.OsKO2 exhibits KO activity, OsKOL 4 does not, instead catalyzing C3a hydroxylation of ''ent''-sandaracopimaradiene and ''ent''-cassadiene, as well as ''ent''-kaurene <ref name="ref2" />.<br />
<br />
===Evolution===<br />
[[File:4.png|right|thumb|150px| Phenotypic Characterization of the gdd1 Mutant.(from reference <ref name="ref4" />)]]<br />
[[File:5.png|right|thumb|150px| Map-Based Cloning of GDD1 and Complementation Test.(from reference <ref name="ref4" />)]]<br />
A rice mutant with greatly decreased height was isolated from a transgene line after ''Agrobacterium tumifaciens''–mediated T-DNA insertion. The mutant, designated ''gibberellin-deficient dwarf1(gdd1)'', retained a stable phenotype and showed segregation of the b-glucuronidase (GUS) gene with the T-DNA insertions until T2 progeny, suggesting that the phenotype was independent of the T-DNA insertion, the mutant with neither the T-DNA insertion nor GUS fragment from the T2 progeny was used for analysis the function of ''GDD1'' .To understand the molecular mechanism responsible for the<br />
gdd1 phenotype, we used a map-based cloning approach to isolate GDD1. All F1 progeny showed a height phenotype similar to that of the wild type. Tests of heterozygotes with F2 progeny yielded a segregation of 401 normal and 144 dwarf plants (x2[3:1] = 0.59 < x20.05= 3.84; P > 0.05), indicating that the dwarf phenotype of the gdd1 mutant is caused by a recessive mutation in a single nuclear gene<ref name="ref4" />.The GA7 gene encodes the enzyme ''ent''-kaurene synthetase A, which catalyzes the conversion of GGPP to CPP. Subcellular localization of the GA1 protein was studied using 35S-labeled GA1 protein and isolated pea chloroplasts. The results showed that the GAl protein is imported into and processed in pea chloroplasts in vitro.DNA sequences of GA7 genomic DNA and cDNA were obtained using the dideoxy method with Sequenase version 2.0 T7 DNA polymerase (U.S.Biochemical Corp.)and both single and double-stranded DNA templates.The 1.4-kb Hindlll DNA in the ga7-9 mutant was amplified by PCR and reamplified by asymmetric PCR, and the single-stranded DNA templates were sequenced directly. The 1.4-kb DNA fragments spanning intron 12 to exon 15 were amplified from genomic DNA isolated from ga7-7 and ga7-4 by PCR. These PCR-amplified DNA products were cloned into the Smal site of the pBluescript SK+ vector, and DNA sequences were obtained by using double-stranded DNA templates isolated from severa1 independent clones<ref name="ref6" />. <br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Department of Biochemistry, Okayama University of Science, Okayama 700–0005, Japan<br />
National Institute for Basic B iology, O kazaki444–8585, Japan<br />
School of Life Science,Grad uate University for Advanced Studies, O kazaki 444–8585, Japan<br />
Department of Botany, Box 91000, Duke University, Durham, North Carolina 27708-1000<br />
Frontier Research Program, The lnstitute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama 351-01, Japan<br />
Research Center for Molecular and Developmental Biology, Key Laboratory of Photosyn thesis and Environ mental Molecular<br />
Physiology, Institute of Botany , Chinese Academy of Sciences , Beijing 100093, China<br />
<br />
==References==<br />
1、Ken-ichiro Hayashi, Keisuke Horie, Yu ji Hiwatashi et al.（2012）Endogenous Diterpenes Derived froment-Kaurene, a Common Gibberellin Precursor, Regulate Protonema Diff erentiation of the Moss ''Physcomitrella patens'' <ref name="ref1[W][OA]" />. Plant Physiology 153:1085-1097.<br />
2、Qiang Wang, Matthew L . Hillwig, Yisheng Wu et al.（2012）CYP701A8: A Rice ent-Kaurene Oxidase P aralog Diverted to More Specialized Diterpenoid Metabolism1<ref name="ref1[W][OA]" />. Plant Physiology 158:1418-1425.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175697Os06g05695002014-06-01T14:03:04Z<p>Baixueluo: /* Labs working on this gene */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
<br />
===Expression==<br />
[[File:1.png|right|thumb|150px| The biosynthetic pathway of GA.(from reference <ref name="ref1" />)]]<br />
[[File:2.png|right|thumb|150px| GA biosynthesis.(from reference <ref name="ref2" />)]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ''ent''-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.The bioactive GAs (GA1and GA4) are synthesized through a series of oxidation reactions of ''ent''-kaurene by two types of oxidases. Both ''ent''-kaurene oxidase and ''ent''-kaurenoic acid oxidase are cytochrome P450 monoo xygenases that success ively convert ''ent''-kaurene to GA12.GA12 is further converted tobioactive GA s by two 2-oxoglutarate-dependent dioxygenases, GA 20-oxidase and GA3-oxidase.GA 2-oxidase is another member of the 2-oxoglutarate-dependent dioxygenase family and is responsible for GA inactivation<ref name="ref1" />.The ring contraction catalyzed by the cytochrome P450 (CYP) ''ent''-kaurenoic acid oxidase (KAO) is then the committed step in GA-specific biosynthesis.OsKO2 exhibits KO activity, OsKOL 4 does not, instead catalyzing C3a hydroxylation of ''ent''-sandaracopimaradiene and ''ent''-cassadiene, as well as ''ent''-kaurene <ref name="ref2" />.<br />
<br />
===Evolution===<br />
[[File:4.png|right|thumb|150px| Phenotypic Characterization of the gdd1 Mutant.(from reference <ref name="ref4" />)]]<br />
[[File:5.png|right|thumb|150px| Map-Based Cloning of GDD1 and Complementation Test.(from reference <ref name="ref4" />)]]<br />
A rice mutant with greatly decreased height was isolated from a transgene line after ''Agrobacterium tumifaciens''–mediated T-DNA insertion. The mutant, designated ''gibberellin-deficient dwarf1(gdd1)'', retained a stable phenotype and showed segregation of the b-glucuronidase (GUS) gene with the T-DNA insertions until T2 progeny, suggesting that the phenotype was independent of the T-DNA insertion, the mutant with neither the T-DNA insertion nor GUS fragment from the T2 progeny was used for analysis the function of ''GDD1'' .To understand the molecular mechanism responsible for the<br />
gdd1 phenotype, we used a map-based cloning approach to isolate GDD1. All F1 progeny showed a height phenotype similar to that of the wild type. Tests of heterozygotes with F2 progeny yielded a segregation of 401 normal and 144 dwarf plants (x2[3:1] = 0.59 < x20.05= 3.84; P > 0.05), indicating that the dwarf phenotype of the gdd1 mutant is caused by a recessive mutation in a single nuclear gene<ref name="ref4" />.The GA7 gene encodes the enzyme ''ent''-kaurene synthetase A, which catalyzes the conversion of GGPP to CPP. Subcellular localization of the GA1 protein was studied using 35S-labeled GA1 protein and isolated pea chloroplasts. The results showed that the GAl protein is imported into and processed in pea chloroplasts in vitro.DNA sequences of GA7 genomic DNA and cDNA were obtained using the dideoxy method with Sequenase version 2.0 T7 DNA polymerase (U.S.Biochemical Corp.)and both single and double-stranded DNA templates.The 1.4-kb Hindlll DNA in the ga7-9 mutant was amplified by PCR and reamplified by asymmetric PCR, and the single-stranded DNA templates were sequenced directly. The 1.4-kb DNA fragments spanning intron 12 to exon 15 were amplified from genomic DNA isolated from ga7-7 and ga7-4 by PCR. These PCR-amplified DNA products were cloned into the Smal site of the pBluescript SK+ vector, and DNA sequences were obtained by using double-stranded DNA templates isolated from severa1 independent clones<ref name="ref6" />. <br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Department of Biochemistry, Okayama University of Science, Okayama 700–0005, Japan<br />
National Institute for Basic B iology, O kazaki444–8585, Japan<br />
School of Life Science,Grad uate University for Advanced Studies, O kazaki 444–8585, Japan<br />
Department of Botany, Box 91000, Duke University, Durham, North Carolina 27708-1000<br />
Frontier Research Program, The lnstitute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama 351-01, Japan<br />
Research Center for Molecular and Developmental Biology, Key Laboratory of Photosyn thesis and Environ mental Molecular<br />
Physiology, Institute of Botany , Chinese Academy of Sciences , Beijing 100093, China<br />
<br />
==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175689Os06g05695002014-06-01T13:56:30Z<p>Baixueluo: /* Evolution */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
<br />
===Expression==<br />
[[File:1.png|right|thumb|150px| The biosynthetic pathway of GA.(from reference <ref name="ref1" />)]]<br />
[[File:2.png|right|thumb|150px| GA biosynthesis.(from reference <ref name="ref2" />)]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ''ent''-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.The bioactive GAs (GA1and GA4) are synthesized through a series of oxidation reactions of ''ent''-kaurene by two types of oxidases. Both ''ent''-kaurene oxidase and ''ent''-kaurenoic acid oxidase are cytochrome P450 monoo xygenases that success ively convert ''ent''-kaurene to GA12.GA12 is further converted tobioactive GA s by two 2-oxoglutarate-dependent dioxygenases, GA 20-oxidase and GA3-oxidase.GA 2-oxidase is another member of the 2-oxoglutarate-dependent dioxygenase family and is responsible for GA inactivation<ref name="ref1" />.The ring contraction catalyzed by the cytochrome P450 (CYP) ''ent''-kaurenoic acid oxidase (KAO) is then the committed step in GA-specific biosynthesis.OsKO2 exhibits KO activity, OsKOL 4 does not, instead catalyzing C3a hydroxylation of ''ent''-sandaracopimaradiene and ''ent''-cassadiene, as well as ''ent''-kaurene <ref name="ref2" />.<br />
<br />
===Evolution===<br />
[[File:4.png|right|thumb|150px| Phenotypic Characterization of the gdd1 Mutant.(from reference <ref name="ref4" />)]]<br />
[[File:5.png|right|thumb|150px| Map-Based Cloning of GDD1 and Complementation Test.(from reference <ref name="ref4" />)]]<br />
A rice mutant with greatly decreased height was isolated from a transgene line after ''Agrobacterium tumifaciens''–mediated T-DNA insertion. The mutant, designated ''gibberellin-deficient dwarf1(gdd1)'', retained a stable phenotype and showed segregation of the b-glucuronidase (GUS) gene with the T-DNA insertions until T2 progeny, suggesting that the phenotype was independent of the T-DNA insertion, the mutant with neither the T-DNA insertion nor GUS fragment from the T2 progeny was used for analysis the function of ''GDD1'' .To understand the molecular mechanism responsible for the<br />
gdd1 phenotype, we used a map-based cloning approach to isolate GDD1. All F1 progeny showed a height phenotype similar to that of the wild type. Tests of heterozygotes with F2 progeny yielded a segregation of 401 normal and 144 dwarf plants (x2[3:1] = 0.59 < x20.05= 3.84; P > 0.05), indicating that the dwarf phenotype of the gdd1 mutant is caused by a recessive mutation in a single nuclear gene<ref name="ref4" />.The GA7 gene encodes the enzyme ''ent''-kaurene synthetase A, which catalyzes the conversion of GGPP to CPP. Subcellular localization of the GA1 protein was studied using 35S-labeled GA1 protein and isolated pea chloroplasts. The results showed that the GAl protein is imported into and processed in pea chloroplasts in vitro.DNA sequences of GA7 genomic DNA and cDNA were obtained using the dideoxy method with Sequenase version 2.0 T7 DNA polymerase (U.S.Biochemical Corp.)and both single and double-stranded DNA templates.The 1.4-kb Hindlll DNA in the ga7-9 mutant was amplified by PCR and reamplified by asymmetric PCR, and the single-stranded DNA templates were sequenced directly. The 1.4-kb DNA fragments spanning intron 12 to exon 15 were amplified from genomic DNA isolated from ga7-7 and ga7-4 by PCR. These PCR-amplified DNA products were cloned into the Smal site of the pBluescript SK+ vector, and DNA sequences were obtained by using double-stranded DNA templates isolated from severa1 independent clones<ref name="ref6" />. <br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Please input related labs here.<br />
<br />
==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175684Os06g05695002014-06-01T13:47:16Z<p>Baixueluo: /* Evolution */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
<br />
===Expression==<br />
[[File:1.png|right|thumb|150px| The biosynthetic pathway of GA.(from reference <ref name="ref1" />)]]<br />
[[File:2.png|right|thumb|150px| GA biosynthesis.(from reference <ref name="ref2" />)]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ''ent''-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.The bioactive GAs (GA1and GA4) are synthesized through a series of oxidation reactions of ''ent''-kaurene by two types of oxidases. Both ''ent''-kaurene oxidase and ''ent''-kaurenoic acid oxidase are cytochrome P450 monoo xygenases that success ively convert ''ent''-kaurene to GA12.GA12 is further converted tobioactive GA s by two 2-oxoglutarate-dependent dioxygenases, GA 20-oxidase and GA3-oxidase.GA 2-oxidase is another member of the 2-oxoglutarate-dependent dioxygenase family and is responsible for GA inactivation<ref name="ref1" />.The ring contraction catalyzed by the cytochrome P450 (CYP) ''ent''-kaurenoic acid oxidase (KAO) is then the committed step in GA-specific biosynthesis.OsKO2 exhibits KO activity, OsKOL 4 does not, instead catalyzing C3a hydroxylation of ''ent''-sandaracopimaradiene and ''ent''-cassadiene, as well as ''ent''-kaurene <ref name="ref2" />.<br />
<br />
===Evolution===<br />
[[File:4.png|right|thumb|150px| Phenotypic Characterization of the gdd1 Mutant.(from reference <ref name="ref4" />)]]<br />
[[File:5.png|right|thumb|150px| Map-Based Cloning of GDD1 and Complementation Test.(from reference <ref name="ref4" />)]]<br />
A rice mutant with greatly decreased height was isolated from a transgene line after ''Agrobacterium tumifaciens''–mediated T-DNA insertion. The mutant, designated ''gibberellin-deficient dwarf1(gdd1)'', retained a stable phenotype and showed segregation of the b-glucuronidase (GUS) gene with the T-DNA insertions until T2 progeny, suggesting that the phenotype was independent of the T-DNA insertion, the mutant with neither the T-DNA insertion nor GUS fragment from the T2 progeny was used for analysis the function of ''GDD1'' .To understand the molecular mechanism responsible for the<br />
gdd1 phenotype, we used a map-based cloning approach to isolate GDD1. The mutant was crossed with 9311, a wild-type polymorphic indica variety. All F1 progeny showed a height phenotype similar to that of the wild type. Tests of heterozygotes with F2 progeny yielded a segregation of 401 normal and 144 dwarf plants (x2[3:1] = 0.59 < x20.05= 3.84; P > 0.05), indicating that the dwarf phenotype of the gdd1 mutant is caused by a recessive mutation in a single nuclear gene<ref name="ref4" />.<br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Please input related labs here.<br />
<br />
==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:7.png&diff=175677File:7.png2014-06-01T13:37:39Z<p>Baixueluo: </p>
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<div></div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175674Os06g05695002014-06-01T13:30:54Z<p>Baixueluo: /* =Expression */</p>
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<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
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===Expression==<br />
[[File:1.png|right|thumb|150px| The biosynthetic pathway of GA.(from reference <ref name="ref1" />)]]<br />
[[File:2.png|right|thumb|150px| GA biosynthesis.(from reference <ref name="ref2" />)]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ''ent''-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.The bioactive GAs (GA1and GA4) are synthesized through a series of oxidation reactions of ''ent''-kaurene by two types of oxidases. Both ''ent''-kaurene oxidase and ''ent''-kaurenoic acid oxidase are cytochrome P450 monoo xygenases that success ively convert ''ent''-kaurene to GA12.GA12 is further converted tobioactive GA s by two 2-oxoglutarate-dependent dioxygenases, GA 20-oxidase and GA3-oxidase.GA 2-oxidase is another member of the 2-oxoglutarate-dependent dioxygenase family and is responsible for GA inactivation<ref name="ref1" />.The ring contraction catalyzed by the cytochrome P450 (CYP) ''ent''-kaurenoic acid oxidase (KAO) is then the committed step in GA-specific biosynthesis.OsKO2 exhibits KO activity, OsKOL 4 does not, instead catalyzing C3a hydroxylation of ''ent''-sandaracopimaradiene and ''ent''-cassadiene, as well as ''ent''-kaurene <ref name="ref2" />.<br />
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===Evolution===<br />
[[File:4.png|right|thumb|150px| Phenotypic Characterization of the gdd1 Mutant.(from reference <ref name="ref4" />)]]<br />
[[File:5.png|right|thumb|150px| Map-Based Cloning of GDD1 and Complementation Test.(from reference <ref name="ref4" />)]]<br />
A rice mutant with greatly decreased height was isolated from a transgene line after ''Agrobacterium tumifaciens''–mediated T-DNA insertion. The mutant, designated ''gibberellin-deficient dwarf1(gdd1)'', retained a stable phenotype and showed segregation of the b-glucuronidase (GUS) gene with the T-DNA insertions until T2 progeny, suggesting that the phenotype was independent of the T-DNA insertion, the mutant with neither the T-DNA insertion nor GUS fragment from the T2 progeny was used for analysis the function of ''GDD1'' <ref name="ref4" />.To understand the molecular mechanism responsible for the<br />
gdd1 phenotype, we used a map-based cloning approach to isolate GDD1. The mutant was crossed with 9311, a wild-type polymorphic indica variety. All F1 progeny showed a height phenotype similar to that of the wild type. Tests of heterozygotes with F2 progeny yielded a segregation of 401 normal and 144 dwarf plants (x2[3:1] = 0.59 < x20.05= 3.84; P > 0.05), indicating that the dwarf phenotype of the gdd1 mutant is caused by a recessive mutation in a single nuclear gene.<br />
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==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:5.png&diff=175672File:5.png2014-06-01T13:29:45Z<p>Baixueluo: uploaded a new version of &quot;File:5.png&quot;</p>
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<div></div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175670Os06g05695002014-06-01T13:23:39Z<p>Baixueluo: /* =Expression */</p>
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<div>Please input one-sentence summary here.<br />
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==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
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===Expression==<br />
[[File:1.png|right|thumb|150px| The biosynthetic pathway of GA.(from reference <ref name="ref1" />)]]<br />
[[File:2.png|right|thumb|150px| GA biosynthesis.(from reference <ref name="ref2" />)]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ''ent''-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.The bioactive GAs (GA1and GA4) are synthesized through a series of oxidation reactions of ''ent''-kaurene by two types of oxidases. Both ''ent''-kaurene oxidase and ''ent''-kaurenoic acid oxidase are cytochrome P450 monoo xygenases that success ively convert ''ent''-kaurene to GA12.GA12 is further converted tobioactive GA s by two 2-oxoglutarate-dependent dioxygenases, GA 20-oxidase and GA3-oxidase.GA 2-oxidase is another member of the 2-oxoglutarate-dependent dioxygenase family and is responsible for GA inactivation<ref name="ref1" />.The ring contraction catalyzed by the cytochrome P450 (CYP) ''ent''-kaurenoic acid oxidase (KAO) is then the committed step in GA-specific biosynthesis.OsKO2 exhibits KO activity, OsKOL 4 does not, instead catalyzing C3a hydroxylation of ''ent''-sandaracopimaradiene and ''ent''-cassadiene, as well as ''ent''-kaurene <ref name="ref2" />.<br />
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===Evolution===<br />
[[File:4.png|right|thumb|150px| GA biosynthesis.(from reference <ref name="ref4" />)]]<br />
A rice mutant with greatly decreased height was isolated from a transgene line after ''Agrobacterium tumifaciens''–mediated T-DNA insertion. The mutant, designated ''gibberellin-deficient dwarf1(gdd1)'', retained a stable phenotype and showed segregation of the b-glucuronidase (GUS) gene with the T-DNA insertions until T2 progeny (see Supplemental Figure 1 online), suggesting that the phenotype was independent of the T-DNA insertion, the mutant with neither the T-DNA insertion nor GUS fragment from the T2 progeny was used for analysis the function of ''GDD1'' <ref name="ref4" />.<br />
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You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:4.png&diff=175669File:4.png2014-06-01T13:23:19Z<p>Baixueluo: uploaded a new version of &quot;File:4.png&quot;</p>
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<div></div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175668Os06g05695002014-06-01T13:13:17Z<p>Baixueluo: /* =Expression */</p>
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<div>Please input one-sentence summary here.<br />
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==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
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===Expression==<br />
[[File:1.png|right|thumb|150px| The biosynthetic pathway of GA.(from reference <ref name="ref1" />)]]<br />
[[File:2.png|right|thumb|150px| GA biosynthesis.(from reference <ref name="ref2" />)]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ''ent''-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.The bioactive GAs (GA1and GA4) are synthesized through a series of oxidation reactions of ''ent''-kaurene by two types of oxidases. Both ''ent''-kaurene oxidase and ''ent''-kaurenoic acid oxidase are cytochrome P450 monoo xygenases that success ively convert ''ent''-kaurene to GA12.GA12 is further converted tobioactive GA s by two 2-oxoglutarate-dependent dioxygenases, GA 20-oxidase and GA3-oxidase.GA 2-oxidase is another member of the 2-oxoglutarate-dependent dioxygenase family and is responsible for GA inactivation<ref name="ref1" />.The ring contraction catalyzed by the cytochrome P450 (CYP) ''ent''-kaurenoic acid oxidase (KAO) is then the committed step in GA-specific biosynthesis.OsKO2 exhibits KO activity, OsKOL 4 does not, instead catalyzing C3a hydroxylation of ''ent''-sandaracopimaradiene and ''ent''-cassadiene, as well as ''ent''-kaurene .<ref name="ref2" />.<br />
===Evolution===<br />
Please input evolution information here.<br />
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You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175667Os06g05695002014-06-01T13:09:59Z<p>Baixueluo: /* =Expression */</p>
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<div>Please input one-sentence summary here.<br />
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==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
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===Expression==<br />
[[File:1.png|right|thumb|150px| The biosynthetic pathway of GA.(from reference <ref name="ref1" />)]]<br />
[[File:2.png|right|thumb|150px| GA biosynthesis(from reference <ref name="ref2" />)]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ''ent''-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.The bioactive GAs (GA1and GA4) are synthesized through a series of oxidation reactions of ''ent''-kaurene by two types of oxidases. Both ''ent''-kaurene oxidase and ''ent''-kaurenoic acid oxidase are cytochrome P450 monoo xygenases that success ively convert ''ent''-kaurene to GA12.GA12 is further converted tobioactive GA s by two 2-oxoglutarate-dependent dioxygenases, GA 20-oxidase and GA3-oxidase.GA 2-oxidase is another member of the 2-oxoglutarate-dependent dioxygenase family and is responsible for GA inactivation<ref name="ref1" />.The ring contraction catalyzed by the cytochrome P450 (CYP) ''ent''-kaurenoic acid oxidase (KAO) is then the committed step in GA-specific biosynthesis. <br />
===Evolution===<br />
Please input evolution information here.<br />
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You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:2.png&diff=175666File:2.png2014-06-01T13:08:20Z<p>Baixueluo: uploaded a new version of &quot;File:2.png&quot;</p>
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<div></div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175665Os06g05695002014-06-01T13:02:52Z<p>Baixueluo: /* =Expression */</p>
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<div>Please input one-sentence summary here.<br />
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==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
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===Expression==<br />
[[File:1.png|right|thumb|150px| The biosynthetic pathway of GA.(from reference <ref name="ref1" />)]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ''ent''-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.The bioactive GAs (GA1and GA4) are synthesized through a series of oxidation reactions of ''ent''-kaurene by two types of oxidases. Both ''ent''-kaurene oxidase and ''ent''-kaurenoic acid oxidase are cytochrome P450 monoo xygenases that success ively convert ''ent''-kaurene to GA12.GA12 is further converted tobioactive GA s by two 2-oxoglutarate-dependent dioxygenases, GA 20-oxidase and GA3-oxidase.GA 2-oxidase is another member of the 2-oxoglutarate-dependent dioxygenase family and is responsible for GA inactivation.<br />
===Evolution===<br />
Please input evolution information here.<br />
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You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175664Os06g05695002014-06-01T13:02:18Z<p>Baixueluo: /* =Expression */</p>
<hr />
<div>Please input one-sentence summary here.<br />
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==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
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===Expression==<br />
[[File:1.png|right|thumb|150px| The biosynthetic pathway of GA.The enzyme names are shown in boldface below or to the right of each arrow.(from reference <ref name="ref1" />)]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ''ent''-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.The bioactive GAs (GA1and GA4) are synthesized through a series of oxidation reactions of ''ent''-kaurene by two types of oxidases. Both ''ent''-kaurene oxidase and ''ent''-kaurenoic acid oxidase are cytochrome P450 monoo xygenases that success ively convert ''ent''-kaurene to GA12.GA12 is further converted tobioactive GA s by two 2-oxoglutarate-dependent dioxygenases, GA 20-oxidase and GA3-oxidase.GA 2-oxidase is another member of the 2-oxoglutarate-dependent dioxygenase family and is responsible for GA inactivation.<br />
===Evolution===<br />
Please input evolution information here.<br />
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You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175663Os06g05695002014-06-01T13:00:36Z<p>Baixueluo: /* =Expression */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
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===Expression==<br />
[[File:1.png|right|thumb|150px| (from reference <ref name="ref1" />)]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ''ent''-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.The bioactive GAs (GA1and GA4) are synthesized through a series of oxidation reactions of ''ent''-kaurene by two types of oxidases. Both ''ent''-kaurene oxidase and ''ent''-kaurenoic acid oxidase are cytochrome P450 monoo xygenases that success ively convert ''ent''-kaurene to GA12.GA12 is further converted tobioactive GA s by two 2-oxoglutarate-dependent dioxygenases, GA 20-oxidase and GA3-oxidase.GA 2-oxidase is another member of the 2-oxoglutarate-dependent dioxygenase family and is responsible for GA inactivation.<br />
===Evolution===<br />
Please input evolution information here.<br />
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==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175661Os06g05695002014-06-01T12:53:13Z<p>Baixueluo: /* =Expression */</p>
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<div>Please input one-sentence summary here.<br />
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==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
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===Expression==<br />
[[File:1.png|right|thumb|150px|]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ent-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.<br />
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===Evolution===<br />
Please input evolution information here.<br />
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You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:1.png&diff=175660File:1.png2014-06-01T12:52:22Z<p>Baixueluo: uploaded a new version of &quot;File:1.png&quot;: Reverted to version as of 12:44, 1 June 2014</p>
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<div></div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:1.png&diff=175659File:1.png2014-06-01T12:51:50Z<p>Baixueluo: uploaded a new version of &quot;File:1.png&quot;</p>
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<div></div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175658Os06g05695002014-06-01T12:49:00Z<p>Baixueluo: /* =Expression */</p>
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<div>Please input one-sentence summary here.<br />
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==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
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===Expression==<br />
[[File:1.jpg]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ent-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.<br />
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===Evolution===<br />
Please input evolution information here.<br />
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You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:1.png&diff=175657File:1.png2014-06-01T12:47:21Z<p>Baixueluo: uploaded a new version of &quot;File:1.png&quot;</p>
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<div></div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175656Os06g05695002014-06-01T12:45:51Z<p>Baixueluo: /* =Expression */</p>
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<div>Please input one-sentence summary here.<br />
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==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
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===Expression==<br />
[[File:1.jpg|right|thumb|150px|]]<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ent-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.<br />
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===Evolution===<br />
Please input evolution information here.<br />
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You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:1.png&diff=175655File:1.png2014-06-01T12:44:22Z<p>Baixueluo: uploaded a new version of &quot;File:1.png&quot;</p>
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<div></div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175646Os06g05695002014-06-01T12:19:58Z<p>Baixueluo: /* =Expression */</p>
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<div>Please input one-sentence summary here.<br />
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==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
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===Expression==<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA biosynthetic pathway.The GA biosynthetic pathway can be classified into three stages. In the first stage, geranylgeranyl diphosphate is cyclized to ''ent-kaurene'' by copalyl diphosphate synthase and ''ent-kaurene'' synthase. In the second stage,''ent-kaurene'' is oxidized by ''ent-kaurene'' oxidase to ''ent-kaurenoic'' acid, which in turn is oxidized by ent-kaurenoic acid oxidase in three steps to GA12<ref name="ref5" />.[[File:Example.jpg]]<br />
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===Evolution===<br />
Please input evolution information here.<br />
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You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175645Os06g05695002014-06-01T12:10:53Z<p>Baixueluo: /* Function */</p>
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<div>Please input one-sentence summary here.<br />
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==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ''ent-kaurene'' oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ''ent-kaurene'' by 2 terpene syntheses, ''ent-copalyl'' diphosphate synthase, and ''ent-kaurene'' synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ''ent-kaurene'' oxidase (KO) and ''ent-kaurenoic'' acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic reticulum<ref name="ref4" />.<br />
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===Expression==<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA bio-synthetic pathway<ref name="ref5" />.<br />
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===Evolution===<br />
Please input evolution information here.<br />
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You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175641Os06g05695002014-06-01T12:05:19Z<p>Baixueluo: /* =Expression */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ent-kaurene oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,<br />
causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ent-kaurene by 2 terpene syntheses, ent-copalyl diphosphate synthase, andent-kaurene synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ent-kaurene oxidase (KO) and ent-kaurenoic acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic<br />
reticulum<ref name="ref4" />.<br />
<br />
===Expression==<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2β-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2β-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function ''Atga2ox7 Atga2ox8'' mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA bio-synthetic pathway<ref name="ref5" />.<br />
<br />
===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175639Os06g05695002014-06-01T12:03:25Z<p>Baixueluo: /* =Expression */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ent-kaurene oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,<br />
causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ent-kaurene by 2 terpene syntheses, ent-copalyl diphosphate synthase, andent-kaurene synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ent-kaurene oxidase (KO) and ent-kaurenoic acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic<br />
reticulum<ref name="ref4" />.<br />
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===Expression==<br />
Degradation of active C19-gibberellins (GAs) by dioxygenases through 2�-hydroxylation yields inactive GA products.We identified two genes in Arabidopsis (''AtGA2ox7'' and ''AtGA2ox8''),using an activation-tagging mutant screen, that encode2-hydroxylases.GA levels in both activation-tagged lines were reduced significantly, and the lines displayed dwarf phenotypes typical of mutants with a GA deficiency.Double loss-of-function Atga2ox7 Atga2ox8 mutants had twofold to fourfold higher levels of active GAs and displayed phenotypes associated with excess GAs, such as early bolting in short days,resistance to the GA biosynthesis inhibitor ancymidol, and decreased mRNA levels of AtGA20ox1,a gene in the GA bio-synthetic pathway<ref name="ref5" />.<br />
<br />
===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175634Os06g05695002014-06-01T11:52:20Z<p>Baixueluo: /* Function */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ent-kaurene oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,<br />
causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.GAs are biosyn-thesized from geranylgranyl diphosph ate, which is converted to ent-kaurene by 2 terpene syntheses, ent-copalyl diphosphate synthase, andent-kaurene synthase, in plants.Then ent-kaurene is converted to GA12 by two cytochrome P450 enzymes, ent-kaurene oxidase (KO) and ent-kaurenoic acid oxidase (KAO). KO locates in the outer membrane of the plastid, and KAO locates in the endoplasmic<br />
reticulum<ref name="ref4" />.<br />
<br />
===Expression==<br />
Please input expression information here.<br />
<br />
===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175632Os06g05695002014-06-01T11:49:34Z<p>Baixueluo: /* Function */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ent-kaurene oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.Therefore,The Rice dwarf virus (RDV), a member of the genus Phytoreovirus,<br />
causes dwarfed growth phenotypes in infected rice ( Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector.P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression.P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development<ref name="ref3" />.<br />
<br />
===Expression==<br />
Please input expression information here.<br />
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===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175563Os06g05695002014-06-01T10:09:52Z<p>Baixueluo: /* Function */</p>
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<div>Please input one-sentence summary here.<br />
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==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway<ref name="ref1" />.All higher plants contain an ent-kaurene oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" /><br />
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===Expression==<br />
Please input expression information here.<br />
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===Evolution===<br />
Please input evolution information here.<br />
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You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175561Os06g05695002014-06-01T10:08:20Z<p>Baixueluo: /* Function */</p>
<hr />
<div>Please input one-sentence summary here.<br />
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==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway[1].All higher plants contain an ent-kaurene oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref5" /><br />
<br />
===Expression==<br />
Please input expression information here.<br />
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===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175560Os06g05695002014-06-01T10:06:53Z<p>Baixueluo: /* Function */</p>
<hr />
<div>Please input one-sentence summary here.<br />
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==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway[1].All higher plants contain an ent-kaurene oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref1" />.<br />
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===Expression==<br />
Please input expression information here.<br />
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===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175558Os06g05695002014-06-01T10:05:34Z<p>Baixueluo: /* Function */</p>
<hr />
<div>Please input one-sentence summary here.<br />
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==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway[1].All higher plants contain an ent-kaurene oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref3" />.<br />
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===Expression==<br />
Please input expression information here.<br />
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===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800&diff=175556Os01g08838002014-06-01T10:04:16Z<p>Baixueluo: /* Function */</p>
<hr />
<div>The rice ''semidwarf-1 (sd1)'' gene is well known as the "green revolution gene" and controls the plant height of rice.<br />
<br />
== Annotated Information ==<br />
=== Function ===<br />
[[File:Example.jpg|right|thumb|150px|''Semidwarf VS. normal-type rice plants at ripening (from reference <ref name="ref3" />).'']]<br />
<br />
This gene is originally derived from the Chinese cultivar Dee-Geo-Woo-Gen (DGWG). It encodes an oxidase enzyme involved in the biosynthesis of gibberellin, which is a plant growth hormone. The rice genome carries at least two GA20ox genes (GA20ox-1 and GA20ox-2). SD1 corresponds to GA20ox-2. Mutation of SD1 will cause a semi-dwarf phenotype of rice without seed yield being affected <ref name="ref1" />. It is not surprising that a rice semidwarfing gene encodes GA20-ox since successful production of semidwarf plants using antisense or overexpressed GA20-ox genes has been reported in Arabidopsis, Solanum dulcamara, potato, and lettuce <ref name="ref3" />.<br />
<br />
It is a key enzyme in the biosynthesis of gibberellin that catalyses the three steps GA53->GA44->GA19->GA20. Impaired GA 20-oxidase activity will cause elevated content of GA53, and reduced amount of G20<ref name="ref3" />. However, there is slight difference between different strains. The extent of GA1 is lower in Doongara (semi-dwarf rice strain) when compared with Kyeema (tall), while there is no significant difference between Calrose76 (semi-dwarf rice strain) and Calrose (tall). Calrose76 has lower contents of GA44 and of GA19 than Calrose, while there is no significant difference between Doongara (semi-dwarf rice strain) and Kyeema (tall) <ref name="ref3" />.<br />
<br />
'''GO assignment(s):''' [http://amigo.geneontology.org/amigo/term/GO:0005506 GO:0005506], [http://amigo.geneontology.org/amigo/term/GO:0016216 GO:0016216], [http://amigo.geneontology.org/amigo/term/GO:0017000 GO:0017000]<br />
<br />
=== Mutation ===<br />
'Dee-Geo-Woo-Gen' (semi-dwarf rice strain): A 383-base-pair deletion from the genome (A 280-bp deletion within the coding region), which induces a frameshift that creates a stop codon in SD1, may be related with the semi-dwarf phenotype <ref name="ref1" /><ref name="ref2" />.<br />
<br />
Calrose76 (semi-dwarf rice strain): The DNA sequence of Calrose76 is identical to Calrose (tall) except for a C to T transition at position 798 that resulted in a change of the predicted amino acid leucine (Leu-266) in Calrose to phenylalanine in Calrose76 <ref name="ref2" />.<br />
<br />
It has been found that introgression of a chromosomal block containing the SD1 allele from tropical japonica is associated with a change in growth patterns in BHA1 (one weedy rice population) <ref name="ref7" />.<br />
<br />
=== Expression ===<br />
This gene is strongly expressed in the leaf blade, stem and unopened flower, whereas GA20ox-1 is predominantly expressed in the unopened flower <ref name="ref1" />. <br />
<br />
Among the DGWG-type sd-1 mutants, IR24 and Habataki have little transcript of this gene, while Milyang 23 expresses a normal or greater amount of truncated transcript. No significant difference is observed between Calrose and its single-nucleotide-substitution mutant Calrose 76 <ref name="ref3" />.<br />
<br />
Analysis of the tissue- and stage-specificity of transcription of sd1 in Nipponbare revealed that this<br />
gene was expressed within 48 hr after sowing, as well as in 10-day-old plants, 30-day-old leaves, and flowering panicles; no transcription is detected in 24-hr-old seedlings or in 14-day-old roots. Transcript accumulates predominantly in adult leaves <ref name="ref3" />.<br />
<br />
{| class='wikitable' style="text-align:center"<br />
|-<br />
! | Primer<br />
! | Forward primer<br />
! | Reverse primer<br />
|-<br />
| rowspan="3"|Gene amplication<br />
| | 5'-CAACTCACTCCCGCTCAACACAGC-3'<br />
| | 5'-TTTGAAATGCAATGTCGTCCACC-3' (used to amplify exon 1 <ref name="ref2" />)<br />
|-<br />
| | 5'-GCGCCAATGGGGTAATTAAAACG-3'<br />
| | 5'-GGCATTCCATTGTTTGTGATTGG-3' (used to amplify exon 2 <ref name="ref2" />)<br />
|-<br />
| | 5'-GTTTGTCCTTGTCGCGTTGCTCAG-3'<br />
| | 5'-TCTGTTCGTTCCGTTTCGTTCCG-3' (used to amplify exon 3 <ref name="ref2" />)<br />
|-<br />
| rowspan="2"|RT-PCR<br />
| | 5'-CAACTCACTCCCGCTCAACACAGC-3'<br />
| | 5'-GTTCGTTCCGTTTCGGTTCCG-3' <ref name="ref3" /><br />
|-<br />
| | 5'-AGCTGGACATGCCCGTGGTC-3'<br />
| | 5'-TTGAGCTGCTGTCCGCGAAG-3' <ref name="ref3" /><br />
|}<br />
<br />
=== Evolution ===<br />
This gene is conserved in ''Arabidopsis'' (47% identity) and pea (50% identity). GA20ox-2 shows 47.8% identity to<br />
GA20ox-1 in rice. There are at least three GA20-ox genes in Arabidopsis <ref name="ref1" /><ref name="ref2" />.<br />
<br />
=== Knowledge Extension ===<br />
<br />
[[File:Gibberellin SD1 RHT Signal.PNG|right|thumb|500px|''Gibberellin signalling pathway (from reference <ref name="ref4" />).'']]<br />
<br />
Except ''sd1'', another ‘green revolution’ gene named ''Rht1'', which encodes a GA signal suppressor DELLA protein. The deletion in the N-terminal region of the native RHT1 constitutively suppresses GA signaling, consequently resulting in a dominant semi-dwarf phenotype <ref name="ref5" />. Both sd1 and Rht1 are associated with GA pathway, indicating the importance of GA in the regulation of developmental processes and making it a prime target for improving crop yield <ref name="ref4" />. <br />
<br />
The wheat green-revolution gene Rht (for ‘reduced height’) <ref name="ref5" /> is a gain-of-function allele caused by a mutation in a transcription factor that is associated with the gibberellin signalling pathway. As wheat has a hexaploid genome, it does not contain recessive alleles such as ''sd1'' in rice that might otherwise be used to produce a semi-dwarf strain of wheat. Although the genetic and biochemical functions of the rice SD1 and wheat RHT proteins are completely different (that is, recessive versus dominant, loss-of-function versus gain-offunction events, enzyme versus transcription factor, respectively), the products of both genes are linked with gibberellin malfunction <ref name="ref1" />.<br />
<br />
In rice, ''Slr1'' gene encodes the DELLA protein. Three semi-dominant dwarf mutants (''Slr1-d1'', ''Slr1-d2'' and ''Slr1-d3'') associated with this gene have been identified, which were caused by gain-of-function mutations in the N-terminal region of SLR1. These three mutants are responsive to GA at a reduced rate, with later SLRl degradation, and showing reduced interaction activity with GID1 (GA receptor) comparing with wild type rice <ref name="ref6" />.<br />
<br />
== Labs working on this gene ==<br />
*Bioscience Center, and Graduate School of Bioagricultural Science, Nagoya University, Nagoya 464-8601, Japan<br />
*Honda R&D, Wako Research Center, Wako 351-0193, Japan<br />
*International Rice Research Institute, Manila, DAPO Box 7777, Philippines<br />
*BioResources Center, and Plant Molecular Biology Laboratory, Riken, Tsukuba 305-0074, Japan<br />
*Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra ACT 2601, Australia<br />
*Plant Genome Center, 1-25-2 Kannondai, Tsukuba, Ibaraki 305-0856, Japan<br />
<br />
== References ==<br />
<references><br />
<ref name="ref1">Sasaki A, Ashikari M, Ueguchi-Tanaka M, Itoh H, Nishimura A, et al. (2002) Green revolution: a mutant gibberellin-synthesis gene in rice. Nature 416: 701-702.</ref><br />
<ref name="ref2">Spielmeyer W, Ellis MH, Chandler PM (2002) Semidwarf (sd-1), "green revolution" rice, contains a defective gibberellin 20-oxidase gene. Proc Natl Acad Sci U S A 99: 9043-9048.</ref><br />
<ref name="ref3">Monna L, Kitazawa N, Yoshino R, Suzuki J, Masuda H, et al. (2002) Positional cloning of rice semidwarfing gene, sd-1: rice "green revolution gene" encodes a mutant enzyme involved in gibberellin synthesis. DNA Res 9: 11-17.</ref><br />
<ref name="ref4">Hedden P. (2003) The genes of the Green Revolution. Trends Genet 19: 5-9.</ref><br />
<ref name="ref5">Peng J, Richards DE, Hartley NM, Murphy GP, Devos KM, et al. (1999) 'Green revolution' genes encode mutant gibberellin response modulators. Nature 400: 256-261.</ref><br />
<ref name="ref6">Asano K, Hirano K, Ueguchi-Tanaka M, Angeles-Shim RB, Komura T, et al. (2009) Isolation and characterization of dominant dwarf mutants, ''Slr1-d'', in rice. Mol Genet Genomics 281: 223-231.</ref><br />
<ref name="ref7">Reagon M, Thurber CS, Olsen KM, Jia Y, Caicedo AL (2011) The long and the short of it: SD1 polymorphism and the evolution of growth trait divergence in U.S. weedy rice. Mol Ecol 20: 3743-3756.</ref><br />
</references><br />
<br />
== Structured Information ==<br />
{{JaponicaGene|<br />
GeneName = Os01g0883800|<br />
Description = Gibberellin 20 oxidase 2 (EC 1.14.11.-) (Gibberellin C-20 oxidase 2) (GA 20-oxidase 2) (Os20ox2) (Semidwarf-1 protein)|<br />
Version = NM_001051549.1 GI:115441468 GeneID:4325003|<br />
Length = 2743 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0883800, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:40138313..40141055|<br />
CDS = 40138313..40138869,40138972..40139293,40140765..40141055|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:40138313..40141055<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:40138313..40141055<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggtggccgagcaccccacgccaccacagccgcaccaaccaccgcccatggactccaccgccggctctggcattgccgccccggcggcggcggcggtgtgcgacctgaggatggagcccaagatcccggagccattcgtgtggccgaacggcgacgcgaggccggcgtcggcggcggagctggacatgcccgtggtcgacgtgggcgtgctccgcgacggcgacgccgaggggctgcgccgcgccgcggcgcaggtggccgccgcgtgcgccacgcacgggttcttccaggtgtccgagcacggcgtcgacgccgctctggcgcgcgccgcgctcgacggcgccagcgacttcttccgcctcccgctcgccgagaagcgccgcgcgcgccgcgtcccgggcaccgtgtccggctacaccagcgcccacgccgaccgcttcgcctccaagctcccatggaaggagaccctctccttcggcttccacgaccgcgccgccgcccccgtcgtcgccgactacttctccagcaccctcggccccgacttcgcgccaatggggagggtgtaccagaagtactgcgaggagatgaaggagctgtcgctgacgatcatggaactcctggagctgagcctgggcgtggagcgaggctactacagggagttcttcgcggacagcagctcaatcatgcggtgcaactactacccgccatgcccggagccggagcggacgctcggcacgggcccgcactgcgaccccaccgccctcaccatcctcctccaggacgacgtcggcggcctcgaggtcctcgtcgacggcgaatggcgccccgtcagccccgtccccggcgccatggtcatcaacatcggcgacaccttcatggcgctgtcgaacgggaggtataagagctgcctgcacagggcggtggtgaaccagcggcgggagcggcggtcgctggcgttcttcctgtgcccgcgggaggacagggtggtgcggccgccgccgagcgccgccacgccgcagcactacccggacttcacctgggccgacctcatgcgcttcacgcagcgccactaccgcgccgacacccgcacgctcgacgccttcacgcgctggctcgcgccgccggccgccgacgccgccgcgacggcgcaggtcgaggcggccagctga</cdnaseq>|<br />
AA = <aaseq>MVAEHPTPPQPHQPPPMDSTAGSGIAAPAAAAVCDLRMEPKIPE PFVWPNGDARPASAAELDMPVVDVGVLRDGDAEGLRRAAAQVAAACATHGFFQVSEHG VDAALARAALDGASDFFRLPLAEKRRARRVPGTVSGYTSAHADRFASKLPWKETLSFG FHDRAAAPVVADYFSSTLGPDFAPMGRVYQKYCEEMKELSLTIMELLELSLGVERGYY REFFADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDVGGLEVLVDGEWRP VSPVPGAMVINIGDTFMALSNGRYKSCLHRAVVNQRRERRSLAFFLCPREDRVVRPPP SAATPQHYPDFTWADLMRFTQRHYRADTRTLDAFTRWLAPPAADAAATAQVEAAS</aaseq>|<br />
DNA = <dnaseqindica>1..557#660..981#2453..2743#atggtggccgagcaccccacgccaccacagccgcaccaaccaccgcccatggactccaccgccggctctggcattgccgccccggcggcggcggcggtgtgcgacctgaggatggagcccaagatcccggagccattcgtgtggccgaacggcgacgcgaggccggcgtcggcggcggagctggacatgcccgtggtcgacgtgggcgtgctccgcgacggcgacgccgaggggctgcgccgcgccgcggcgcaggtggccgccgcgtgcgccacgcacgggttcttccaggtgtccgagcacggcgtcgacgccgctctggcgcgcgccgcgctcgacggcgccagcgacttcttccgcctcccgctcgccgagaagcgccgcgcgcgccgcgtcccgggcaccgtgtccggctacaccagcgcccacgccgaccgcttcgcctccaagctcccatggaaggagaccctctccttcggcttccacgaccgcgccgccgcccccgtcgtcgccgactacttctccagcaccctcggccccgacttcgcgccaatggggtaattaaaacgatggtggacgacattgcatttcaaattcaaaacaaattcaaaacacaccgaccgagattatgctgaattcaaacgcgtttgtgcgcgcaggagggtgtaccagaagtactgcgaggagatgaaggagctgtcgctgacgatcatggaactcctggagctgagcctgggcgtggagcgaggctactacagggagttcttcgcggacagcagctcaatcatgcggtgcaactactacccgccatgcccggagccggagcggacgctcggcacgggcccgcactgcgaccccaccgccctcaccatcctcctccaggacgacgtcggcggcctcgaggtcctcgtcgacggcgaatggcgccccgtcagccccgtccccggcgccatggtcatcaacatcggcgacaccttcatggtaaaccatctcctattctcctctcctctgttctcctctgcttcgaagcaacagaacaagtaattcaagcttttttttctctctcgcgcgaaattgacgagaaaaataagatcgtggtaggggcggggctttcagctgaaagcgggaagaaaccgacctgacgtgatttctctgttccaatcacaaacaatggaatgccccactcctccatgtgttatgatttatctcacatcttatagttaataggagtaagtaacaagctacttttttcatattatagttcgtttgattttttttttttaaagtttttttagttttatccaaatttattgaaaaacttagcaacgtttataataccaaattagtctcatttagtttaatattgtatatattttgataatatatttatgttatattaaaaatattactatatttttctataaacattattaaaagccatttataatataaaatggaaggagtaattaatatggatctcccccgacatgagaatattttccgatggtgtgacgacgccatgtaagcttcggtgggcctggacggccagaggtgccaacagccacgtccaacaacccctgggtccccccctaacactccaaacagtagtgagtagtgtctcgtcgcgttttagtatttgatgacaaacaaagtgtgagttgagttagccaccaccaacttgcacacgagcacatacatttgtgtccattctcgccagtcacttccatctctagtcctaactcctatctagcgatgtaagcggataatttcatcatccgtatataaacctgtttgttatagttaatttcctatataatactataacagtatacattttaaaagaaaacaaaattaggataaacaggccctgctcctatccatccatggcacttggaaggaccagactcggtcatgccatgccaagccaagatatgggttatggaagagtagagaagaggagagatgagagataagcatgcgttctcctcctcgttggatgtgtattttggagggatttgtgtagtagtagcagcggcgccgcggggacggatgcggatggtggcgctttcggtggcgttttcccgggggggttttggtttggcgcttgggggggatggcatggcgcggcgtgcggctgcacgccacacacacgcgcgcgcacgcacgtacgtcgtcgtcgccgcgggcggacggtagcttagggtggtgtgttccgcgcgcgggcgcggattgttccatgccgatcgatttggcgccaccctcgccgcggctcttgtcgcgtcgtgcgcctctctcgcgcggtttgtccttgtcgcgttgctcagccggcgacgggggcacggacattggcgatgtagccctgcacgtgtcggcctctccgttgatgaatgatgatgtatgtatgtatttttttttgtctgaaggaatttgtggggaattgttgtgtgtgcaggcgctgtcgaacgggaggtataagagctgcctgcacagggcggtggtgaaccagcggcgggagcggcggtcgctggcgttcttcctgtgcccgcgggaggacagggtggtgcggccgccgccgagcgccgccacgccgcagcactacccggacttcacctgggccgacctcatgcgcttcacgcagcgccactaccgcgccgacacccgcacgctcgacgccttcacgcgctggctcgcgccgccggccgccgacgccgccgcgacggcgcaggtcgaggcggccagctga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051549.1 RefSeq:Os01g0883800]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175555Os06g05695002014-06-01T10:03:54Z<p>Baixueluo: /* Function */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway[1].All higher plants contain an ent-kaurene oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (''OsKO2/CYP701A6'') is required for GA biosynthesis <ref name="ref2" />.<br />
<br />
===Expression==<br />
Please input expression information here.<br />
<br />
===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Please input related labs here.<br />
<br />
==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175552Os06g05695002014-06-01T09:56:37Z<p>Baixueluo: /* Function */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway[1].All higher plants contain an ent-kaurene oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( ''Oryza sativa'') contains five CYP701A subfamily members in its genome,despite the fact that only one (OsKO2/CYP701A6) is required for GA biosynthesis[2].<br />
<br />
===Expression==<br />
Please input expression information here.<br />
<br />
===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Please input related labs here.<br />
<br />
==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175530Os06g05695002014-06-01T09:24:20Z<p>Baixueluo: /* Function */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway[1].All higher plants contain an ent-kaurene oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( Oryza sativa) contains five CYP701A subfamily members in its genome,despite the fact that only one (OsKO2/CYP701A6) is required for GA biosynthesis[2].<br />
<br />
===Expression==<br />
Please input expression information here.<br />
<br />
===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Please input related labs here.<br />
<br />
==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175529Os06g05695002014-06-01T09:23:00Z<p>Baixueluo: /* Annotated Information */</p>
<hr />
<div>Please input one-sentence summary here.<br />
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==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway[1].All higher plants contain an ent-kaurene oxidase (KO), as such a cytochrome P450 (CYP) 701 family member is required for gibberellin (GA) phytohormone biosynthesis. While gene expansion and functional diversification of GA-biosynthesis-derived diterpene synthases into more specialized metabolism has been demonstrated, no functionally divergent KO/CYP701 homologs have been previously identified. Rice ( Oryza sativa) contains five CYP701A subfamily members in its genome,despite the fact that only one (OsKO2/CYP701A6) is required for GA biosynthesis[2].<br />
<br />
<br />
Physcomitrella patens.<br />
===Expression==<br />
Please input expression information here.<br />
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===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluohttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0569500&diff=175522Os06g05695002014-06-01T09:12:41Z<p>Baixueluo: /* Annotated Information */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized froment-kaurene via ent- kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway. No GAs or functionally orthologous GID1-DELLA components have been found in the moss Physcomitrella patens.P. patens produces ent-kaurenoic acid, an ent-kaurene metabolite in the GA biosynthesis pathway. <br />
<br />
Physcomitrella patens.<br />
===Expression===<br />
Please input expression information here.<br />
<br />
===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0569500|<br />
Description = Similar to Ent-kaurene oxidase 1 (Fragment)|<br />
Version = NM_001064440.1 GI:115468611 GeneID:4341343|<br />
Length = 6995 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0569500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:22898131..22905125|<br />
CDS = 22898131..22898642,22898989..22899157,22899243..22899497,22899652..22899792,22899893..22900051<br>,22901401..22901561,22904990..22905125|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:22898131..22905125<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</cdnaseq>|<br />
AA = <aaseq>MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPP PAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMI DKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRE RMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREIS KDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIA KNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDD TTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAG RRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPRG RM</aaseq>|<br />
DNA = <dnaseqindica>6484..6995#5969..6137#5629..5883#5334..5474#5075..5233#3565..3725#1..136#atggagtcgatgctcgtagccggagcgggcgcggcggcggtggcggccgtcgggggcctcgtcgcggcggccgcgctcgccgacaagctcgtcgcggcgccgccgccgcgcaagaaccgcgccaacccgcctccaggtaattaattaatccagctcgccgtgttcgtttcgatcgatcaaatgatcttctccaattggtttgatcctcactgctgctgtgtctcggtgttcgtcgtactctctccattgattgatctctgttggagatggcagtgtatgtggttagtttgttcgtcggaaaatatgtcgaattttctctgtttcagctagtgcgtaagctttttttttttgatggaatggtaatttctggtgtcgcaacaattagaaatgagcatgaatgatcagtttggcgtagataacaggatttgagctataacagcactcacggctgcgacagcacacctattgtgcacaaacaagtactccgccgttctaaaacataagtattttttttacatgtacacgatctccgagatgatactttaatcaacaatatctacaaaagtaaaatgtcttaaataaaaagagttgcatattatgatagttcgtttaatgataaatataataaaattaaatttacatgattaatcttttttaaaaagtttgacttagcactgttctaaaaatgcttatattttgggacagagggagtaatataaatggaggaggtggttagtggttttggcagatgtaaatatagtataattgtagtgtaattacattgtaacttacatataactacgatataaatttgaaccgttcgatttgctttaaaattcgcaagggatgtaggaaaaaatcacaacccactcacatgtgaggcgattcagtaccattacctccgttttcacataagtagcacatcacctagggatttttgaaaattacatactccctccgagctgatgatattagtcgctttaaataaggataaaatcaaaccttaaaatctttgactataaataatttctaaaatatttatcttaaaaaatgaaaaccatatatatatatatatatatatatatatatatatattagtcttaaaaagtacttcaataaaatcatatatttgttgatatttctatatatattataatagaaaatagttatcaaagctactctttggagactgtgcccttgtccaaaacgacaaatattattaacctggagggagtacaagttaaactatagttacatgtaagttacgttgtaattacatcacggtatttacacctatcaaattttttagcgaaaatttaacgacaaatgtatagctaatcccttaattttgatatattgcttgaaaatcgaattttccgaacagcaatttgtcgcgatggaccatataggaattaaagtggataatccaaatccactagattttattgaaactatatatcctaatcctaatactaatccgttggattttgaaaaaatacttaccatatctccgttcgattggattcagaacggatcagattgaaaagtgttaaatcatttttcatccttttttttttgagaaatcctttttttttctgtagtcctcggttcagttttgactgaatgtctgaaagggatgttgtttaattaacttgaaacagttcaaaattacaagcattcatgtctctgtcagttgaagtagccttctccttctcttctatatgttcgtcagactacttcagaaagtaggtacttcctctagtcacgaatatgttactgagaggctataactaaaaacacagtacctctgatttgtaatagataatgcccttaacatttgaaaatatgtttactcatccatcttatttatagaatttatataattattatttattttttgagttattttattattagaaatattttaagcataattcatatcttattcacttacccaaaatttttaaataagacgaatagtcaaacgtgtatcaaaaattaactgagccggagagaggatatctgaaactatgaaaagaggagcaattaaccatttcttccttaatccgcctgcatgcaacttgaaatcacaaaagcaaactcaagtcaatgccatggggaaaatttacagtagcccctgcttgcaggtaccaaaagaaaattgatcaatttcatgcaggtgtgaccaaagtactggattccaggcatagcacaagaacattccacggaagtaccacagcaaactcaatcaatcgttttacttggtccaaaagaattaagaaaactgtgcacctcttaacccattctaatgatgaggatccatttcctaattaaaggactttgttgtcaatcaattagactatattaataaagatgagccatttattttatcttttttttttttgaaaaatgttgaccagagaagagaaacatcccaaaggcattgccatcttacctgtatacggactctgttgccaccgttcttaaaaacttttaaaatgattttcattttctacggaaaagagtattaatgtagaatagtaaaaaaaatcattatcagtatttccgacggtggcggggagttatataatatatatttatgatgaattgtattcccctgcttttgttgaaacaaagtgttctttcaatatctcattcactattcatttgacttatatggattttaaaaataggcaatttatatgccataccgttatccattctgagcatggactaaatatttagaacgtaaattttgaacagactaaccttttagtgtgccggaactattaaaaaaaattaaagacttttattaatccataaaatatgtccgaaaatgtgttcttcagtttgttttcttctggttaagtatgcactggttctagttggcacatgtataatagttgtgattgcatatctattttgctgattaagagtaaacaaatggaaataactttctttgagttacgctttcagttaagattgttttgaaattattaatgtgcaaaatgaaagtagttattatagatcctttcagatatgtctatttcgatgagccactagtgcctgtgttgaaaatgcaaattattctagacaaattttgctagaggactttgtgacttcgtgattttagctgtatgacactgcacgaagtaactttgggggacaacactctataaacgtgaaaattgactcgtcgacaccgcttctattagaatattcattccaggttgaatatgagagtgaaatcatgtgaaagttctgaaatgcctctgagcctacatgtgaggtctaccatctctctatcactggccaaaatttcatcagatatttttagcttatttgattgtgtttactccatctgtccatcatctatccatgtacatagctagcattcttactgaaaaatgacccaataaaaggaaatatagaacctgtacatgttaaattacttgcttgaatgtttggtgttttcatttatgcagagacgaattaccaaaaacaaattaatttcagtccactacaagagctttagtaataacatcgttagttattgtaatgcagctgttcctggtttacccattattggaaatctgcatcaattgaaagaaaagaagcctcatcagacgtttgcaaaatggtctgaaacttatggaccaatctacactataaagaccggagcttctccagtggttgtgctcaattcaactgaagtagccaaggaggtatgcaagttgtcctttttcagcatttttttgttacctgttaatgttttcattttctttccgactacccaaattggtgtccccatatactctccactcttttacccacacttttcattcactgatatcctggactagcaccacaatttgctcgagctaagggtgctggtggtccatctttgttaggaaataaaataaaattatcatgactgttgcatcaagcaatgcatgtattcttacataattgtaactctggtgaccgcatactgttataaaatcaacctatccgacattacaactccaagtcttaaattataaccgactttatacacatattcgacacaaactctaacaaataaagtaataactaattcaatagaaacaaacatagacttatatggttcaaaatttaatcttagttttccccttttttttcaaaattttcacttaatctatctttagactcttttgaatttctattcaaacagataaaactctgaagagcaaggaattcaaaagagtacaaatatgtgatgtttttttttttaaaaaagatgaattcaaactgatgaaatttagaataaataggcaaggtttcagattgatcaactcgaaaaaaattgctctaaaatgctaaaacacgaatgaaggggtagttaaggacaaggaagcacacgtggttacagttgtaccattgtttttacgcttttcaaattatccttacacatgcaaaatagaggtgcttttagtatgatgtgtatgtaaattgcatggttttgacaatttcaaatgctatcctatccggttacttagtttacagttgatttttttacttgtgcaaaagtttagaattgtaagtggacatttctcttgataaattaaatgtgaatatcataattacaattaattcagatcagaaatgaaattaacctacaacatgaactctagagctaatatgatattattttttgtttctcttttttcgtggaggtaaaactgtaaacgctttggttcatgcaattgtgctatgatgttggcttctagttcatgacatgggtcctatgatgtttgatatgttttatcttccttattattcttggcttcaaggttgacatgtttgtatatgtttgtgcaaataccttgtttggaaagaaaataaaggcaatgtaaggaataacatcccaaagacttgaactctgtttgatcttcaatatatctaagtcttcaaaaaagaacttttatactacttttttcttactcttgcatagttacatatactcaataatgataatctcatattaattggtctgtaaataacaaaaacacatattctgtactactttttccaggcgatgattgacaaattctcatccatatctactcgaaagctaccaaaagcaatgtctgtgctaactcgtaaaagtatggtcgcaatcagcgactacggtgactaccaaaagatggcgaagcgtaatattatgattggcatgttaggttttaatgcacaggtacaaatctcagcaaaatttaaattcaatagtggattttgggtaccattagcactcagttaattgagctctaacaatgaaatgcttgcataaatttcagaaacagtttcgcggtacaagagagaggatgatcagtaacgtgttaagcactttgcataagttggtttctcttgacccacattcccctctgaacttcagggatgtttacattaatgagctgttcagcttgtccttgatccaggtttgtattacctctgaacttgaactctccagtggtccgtttgttacttccaatacctgtcatgattgttatgaacttataattgttgcaatttcgtagttactaggactgatcttgattgttatgaacttatatatatttgttacaatttcagagtttaggtgaggatgtgagttcagtttatgtggaagagtttgggagggagatatccaaggacgaaatctttgatgtccttgtgcatgagatgatgatgtgtgcagttgaggctgactggagggactacttcccctacctcagctggcttccaaacaagagcttcgacacaattgtgtctactacagaattcagacgagatgctatcatgaatgcattgatcaagaagcagaaggagaggattgcacgcggagaggtgacacaaaacttctattcgtatccaaaaataactttttttttctgcagatcatctgaactgaactgtaacgtattgcatgcaggcaagggcatcctacattgacttcttgctggaagctgagaggagtgcacagctgacagatgaccaactgatgctgctgctgtcggagtccatcctggctgcagctgatactgtcctggtgaccaccgaatggaccatgtatgagattgccaagaaccctgacaaacaggcaagaaattaactgatactacctccagttttattttttggtttcggacaacacatgttttagcatgttttctttgtaacttgtcgtaaacgtcaattaattttgtccggagggagtactttcaattatcttttgtatatactctttcttgtattgtattgtgatcctctgacataactttctcttgatatggtcattgacatgtcagcgatcatgtctctataactttacgagaaagaaagtcagaggatctcaatccctctttctttcaaatgcatttcagcattttgttgccaaccaaactaataaacaattgattgtttcttgttcttggcacatcgatcaggagctactctaccaagagatccgagaggcgtgcggcggcgaggcggtgaccgaggacgacttgccgcggctgccgtacctcaacgccgtgttccacgagacgctgcggctgcactccccggtgccggtgctgcccccgaggttcgtccacgacgacaccacgctcgccggctacgacatcgcggcgggcacccagatgatgatcaacgtgtacgcgtgccacatggacgagaaggtgtgggagtcgccgggggagtggtcgccggagaggttcctcggcgaggggttcgaggtggcggacaggtacaagacgatggcgttcggcgccgggaggaggacctgcgcggggagcctgcaggcgatgaacatcgcgtgcgtcgccgtggcgcgcctcgtgcaggagctcgagtggaggctgagggagggcgacggggacaaggaggacaccatgcagttcaccgccttgaagcttgacccgctgcatgtccacctcaagcccagaggaaggatgtga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064440.1 RefSeq:Os06g0569500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Baixueluo