http://192.168.164.12:81/ricewiki/api.php?action=feedcontributions&feedformat=atom&user=Ban+BuzhuRiceWiki - User contributions [en]2026-08-27T14:35:53ZUser contributionsMediaWiki 1.30.0https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=171450Os01g02017002014-05-24T01:50:02Z<p>Ban Buzhu: </p>
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<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.(B) Panicle at the heading stage.(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .(G) and (H) Pollen grains at stage 12 stained by I2-KI.(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.<br />
MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]<br />
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[[File:Figure 3. Analyses of the MT-1-4b-amiRNA Lines.png|right|thumb|320px|'''Figure 2.''' ''Analyses of the MT-1-4b-amiRNA Lines. <ref name="ref1" />.'']]<br />
[[File:Figure 4. Molecular Identification of Rice MADS3..png|right|thumb|320px|'''Figure 3.''' ''Molecular Identification of Rice MADS3. <ref name="ref1" />'']]<br />
[[File:Figure 5. Rice MADS3 Expression Pattern Analysis..png|right|thumb|320px|'''Figure 4.''' Rice MADS3 Expression Pattern Analysis. <ref name="ref1" />'']]<br />
[[File:Figure 6. Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily.png|right|thumb|320px|'''Figure 5.''' Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily. <ref name="ref2" />'']]<br />
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'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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*'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.<br />
*'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.<br />
*'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.<br />
*The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br><br />
*mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs<ref name="ref2" />.<br />
* mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. <br />
*mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable '''OsMADS3''' transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />.<br />
* mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
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===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
*[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
*the ABC model can also partially explain how stamen determination is specified in the monocot plant rice <ref name="ref2" />. For example, a rice B-class gene, SUPERWOMEN1 (SPW1 or MADS16), which is orthologous to the Arabidopsis APETALA3 gene, has been shown to be crucial for stamen specification . spw1 mutants show homeotic conversions of stamens to carpels and lodicules to palea/lemma-like structures. In Arabidopsis, the C-class gene AGAMOUS (AG) acts to specify stamen and carpel identities and floral meristem determinacy . Studies in rice identified two C-class MADS box genes, MADS3 and MADS58, that may have distinct functions in specifying stamen identity, with MADS3 playing a more important role <ref name="ref2" />. <br />
*Other MADS box genes, such as SPOROCYTELESS/NOZZLE (SPL/NZZ) <ref name="ref5" /><ref name="ref6" />.andAG from Arabidopsis and MADS2 from maize <ref name="ref6" />, have been implicated in regulating anther development. SPL/NZZ regulates the formation of anther walls and pollen mother cells, as the primary sporogenous cells cannot form pollen mother cells in spl anthers, thereby blocking early cell differentiation [5].AGhas been shown to activate the expression of SPL/NZZ, suggesting that this gene is necessary for early stamen development <ref name="ref7" />. During later developmental stages, AG continues its expression in the anther and regulates anther dehiscence by directly regulating the expression of the gene that encodes a jasmonic acid (JA) synthetic enzyme, DEFECTIVE IN ANTHER DEHISCENCE1 <ref name="ref7" />. Maize MADS2 is required for anther dehiscence and pollen maturation, and knockdown of MADS2 resulted in abortion of anthers and defective pollen development<ref name="ref7" />..<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
<ref name="ref5"><br />
Yang, Y., Fanning, L., and Jack, T. (2003). The K domain mediates heterodimerization of the Arabidopsis floral organ identity proteins, APETALA3 and PISTILLATA. Plant J. 33: 47–59.</ref><br />
<ref name="ref6"><br />
Schiefthaler, U., Balasubramanian, S., Sieber, P., Chevalier, D.,Wisman, E., and Schneitz, K. (1999). Molecular analysis of NOZZLE,a gene involved in pattern formation and early sporogenesis duringsex organ development in Arabidopsis thaliana. Proc. Natl. Acad. Sci.USA 96: 11664–11669.</ref><br />
<ref name="ref7"><br />
Itoh, J., Nonomura, K., Ikeda, K., Yamaki, S., Inukai, Y., Yamagishi, H., Kitano, H., and Nagato, Y. (2005). Rice plant development: From zygote to spikelet. Plant Cell Physiol. 46: 23–47.</ref><br />
</references><br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttc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/>
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=171449Os01g02017002014-05-24T01:48:41Z<p>Ban Buzhu: </p>
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<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.(B) Panicle at the heading stage.(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .(G) and (H) Pollen grains at stage 12 stained by I2-KI.(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.<br />
MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]<br />
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[[File:Figure 3. Analyses of the MT-1-4b-amiRNA Lines.png|right|thumb|320px|'''Figure 2.''' ''Analyses of the MT-1-4b-amiRNA Lines. <ref name="ref1" />.'']]<br />
[[File:Figure 4. Molecular Identification of Rice MADS3..png|right|thumb|320px|'''Figure 3.''' ''Molecular Identification of Rice MADS3. <ref name="ref1" />'']]<br />
[[File:Figure 5. Rice MADS3 Expression Pattern Analysis..png|right|thumb|320px|'''Figure 4.''' Rice MADS3 Expression Pattern Analysis. <ref name="ref1" />'']]<br />
[[File:Figure 6. Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily.png|right|thumb|320px|'''Figure 5.''' Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily. <ref name="ref2" />'']]<br />
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'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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*'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.<br />
*'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.<br />
*'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.<br />
*The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br><br />
*mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs<ref name="ref2" />.<br />
* mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. <br />
*mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable '''OsMADS3''' transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />.<br />
* mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
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===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
*[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
* the ABC model can also partially explain how stamen determination is specified in the monocot plant rice <ref name="ref2" />. For example, a rice B-class gene, SUPERWOMEN1 (SPW1 or MADS16), which is orthologous to the Arabidopsis APETALA3 gene, has been shown to be crucial for stamen specification . spw1 mutants show homeotic conversions of stamens to carpels and lodicules to palea/lemma-like structures. In Arabidopsis, the C-class gene AGAMOUS (AG) acts to specify stamen and carpel identities and floral meristem determinacy . Studies in rice identified two C-class MADS box genes, MADS3 and MADS58, that may have distinct functions in specifying stamen identity, with MADS3 playing a more important role <ref name="ref2" />. <br />
*Other MADS box genes, such as SPOROCYTELESS/NOZZLE (SPL/NZZ) <ref name="ref5" /><ref name="ref6" />.andAG from Arabidopsis and MADS2 from maize <ref name="ref6" />, have been implicated in regulating anther development. SPL/NZZ regulates the formation of anther walls and pollen mother cells, as the primary sporogenous cells cannot form pollen mother cells in spl anthers, thereby blocking early cell differentiation [5].AGhas been shown to activate the expression of SPL/NZZ, suggesting that this gene is necessary for early stamen development <ref name="ref7" />. During later developmental stages, AG continues its expression in the anther and regulates anther dehiscence by directly regulating the expression of the gene that encodes a jasmonic acid (JA) synthetic enzyme, DEFECTIVE IN ANTHER DEHISCENCE1 <ref name="ref7" />. Maize MADS2 is required for anther dehiscence and pollen maturation, and knockdown of MADS2 resulted in abortion of anthers and defective pollen development<ref name="ref7" />..<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
<ref name="ref5"><br />
Yang, Y., Fanning, L., and Jack, T. (2003). The K domain mediates heterodimerization of the Arabidopsis floral organ identity proteins, APETALA3 and PISTILLATA. Plant J. 33: 47–59.</ref><br />
<ref name="ref6"><br />
Schiefthaler, U., Balasubramanian, S., Sieber, P., Chevalier, D.,Wisman, E., and Schneitz, K. (1999). Molecular analysis of NOZZLE,a gene involved in pattern formation and early sporogenesis duringsex organ development in Arabidopsis thaliana. Proc. Natl. Acad. Sci.USA 96: 11664–11669.</ref><br />
<ref name="ref7"><br />
Itoh, J., Nonomura, K., Ikeda, K., Yamaki, S., Inukai, Y., Yamagishi, H., Kitano, H., and Nagato, Y. (2005). Rice plant development: From zygote to spikelet. Plant Cell Physiol. 46: 23–47.</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170937Os01g02017002014-05-22T16:49:01Z<p>Ban Buzhu: </p>
<hr />
<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
<br />
==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.(B) Panicle at the heading stage.(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .(G) and (H) Pollen grains at stage 12 stained by I2-KI.(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.<br />
MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]<br />
<br />
[[File:Figure 3. Analyses of the MT-1-4b-amiRNA Lines.png|right|thumb|320px|'''Figure 2.''' ''Analyses of the MT-1-4b-amiRNA Lines. <ref name="ref1" />.'']]<br />
[[File:Figure 4. Molecular Identification of Rice MADS3..png|right|thumb|320px|'''Figure 3.''' ''Molecular Identification of Rice MADS3. <ref name="ref1" />'']]<br />
[[File:Figure 5. Rice MADS3 Expression Pattern Analysis..png|right|thumb|320px|'''Figure 4.''' Rice MADS3 Expression Pattern Analysis. <ref name="ref1" />'']]<br />
[[File:Figure 6. Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily.png|right|thumb|320px|'''Figure 5.''' Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily. <ref name="ref2" />'']]<br />
<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
<br />
*'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.<br />
*'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.<br />
*'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.<br />
*The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br><br />
*mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs<ref name="ref2" />.<br />
* mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. <br />
*mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable '''OsMADS3''' transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />.<br />
* mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
<br />
===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
<br />
===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
<br />
===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
<br />
==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
<br />
*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
<br />
*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
<br />
*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
<br />
*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
<br />
*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
<br />
==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170934Os01g02017002014-05-22T16:47:10Z<p>Ban Buzhu: </p>
<hr />
<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
<br />
==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.(B) Panicle at the heading stage.(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .(G) and (H) Pollen grains at stage 12 stained by I2-KI.(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.<br />
MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]<br />
<br />
[[File:Figure 3. Analyses of the MT-1-4b-amiRNA Lines.png|right|thumb|320px|'''Figure 2.''' ''Analyses of the MT-1-4b-amiRNA Lines. <ref name="ref1" />.'']]<br />
[[File:Figure 4. Molecular Identification of Rice MADS3..png|right|thumb|320px|'''Figure 3.''' ''Molecular Identification of Rice MADS3. <ref name="ref1" />'']]<br />
[[File:Figure 5. Rice MADS3 Expression Pattern Analysis..png|right|thumb|320px|'''Figure 4.''' Rice MADS3 Expression Pattern Analysis. <ref name="ref1" />'']]<br />
[[File:Figure 6. Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily.png|right|thumb|320px|'''Figure 5.''' Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily. <ref name="ref2" />'']]<br />
[[File:Figure 3. Analyses of the MT-1-4b-amiRNA Lines.png|right|thumb|320px|'''Figure 5.''' ''123 <ref name="ref1" />.'']]<br />
<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
<br />
*'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.<br />
*'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.<br />
*'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.<br />
*The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br><br />
*mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs<ref name="ref2" />.<br />
* mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. <br />
*mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable '''OsMADS3''' transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />.<br />
* mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
<br />
===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
<br />
===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
<br />
===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
<br />
==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
<br />
*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
<br />
*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
<br />
*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
<br />
*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
<br />
*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
<br />
*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
<br />
==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170921Os01g02017002014-05-22T16:26:52Z<p>Ban Buzhu: </p>
<hr />
<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
<br />
==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.(B) Panicle at the heading stage.(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .(G) and (H) Pollen grains at stage 12 stained by I2-KI.(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.<br />
MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]<br />
<br />
[[File:Figure 3. Analyses of the MT-1-4b-amiRNA Lines.png|right|thumb|320px|'''Figure 2.''' ''Analyses of the MT-1-4b-amiRNA Lines. <ref name="ref1" />.'']]<br />
[[File:Figure 4. Molecular Identification of Rice MADS3..png|right|thumb|320px|'''Figure 3.''' ''Molecular Identification of Rice MADS3. <ref name="ref1" />'']]<br />
[[File:Figure 5. Rice MADS3 Expression Pattern Analysis..png|right|thumb|320px|'''Figure 4.''' Rice MADS3 Expression Pattern Analysis. <ref name="ref1" />'']]<br />
[[File:Figure 6. Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily.png|right|thumb|320px|'''Figure 5.''' Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily. <ref name="ref2" />'']]<br />
<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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*'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.<br />
*'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.<br />
*'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.<br />
*The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br><br />
*mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs<ref name="ref2" />.<br />
* mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. <br />
*mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable '''OsMADS3''' transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />.<br />
* mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
<br />
===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
<br />
*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
<br />
*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
<br />
==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170920Os01g02017002014-05-22T16:25:28Z<p>Ban Buzhu: </p>
<hr />
<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
<br />
==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.(B) Panicle at the heading stage.(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .(G) and (H) Pollen grains at stage 12 stained by I2-KI.(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.<br />
MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]<br />
<br />
[[File:Figure 3. Analyses of the MT-1-4b-amiRNA Lines.png|right|thumb|320px|'''Figure 2.''' ''Analyses of the MT-1-4b-amiRNA Lines. <ref name="ref1" />.'']]<br />
[[File:Figure 4. Molecular Identification of Rice MADS3..png|right|thumb|320px|'''Figure 3.''' ''Molecular Identification of Rice MADS3. <ref name="ref1" />'']]<br />
[[File:Figure 5. Rice MADS3 Expression Pattern Analysis..png|right|thumb|320px|'''Figure 4.''' Rice MADS3 Expression Pattern Analysis. <ref name="ref1" />'']]<br />
[[File:Figure 6. Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily.png|right|thumb|320px|'''Figure 5.''' Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily. <ref name="ref2" />'']]<br />
<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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*'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.<br />
*'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.<br />
*'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.<br />
*The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br><br />
*mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs<ref name="ref2" />.<br />
* mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. <br />
*mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />.<br />
* mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
<br />
===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
<br />
*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
<br />
*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
<br />
*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
<br />
==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170917Os01g02017002014-05-22T16:23:02Z<p>Ban Buzhu: </p>
<hr />
<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
<br />
==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.(B) Panicle at the heading stage.(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .(G) and (H) Pollen grains at stage 12 stained by I2-KI.(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.<br />
MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]<br />
<br />
[[File:Figure 3. Analyses of the MT-1-4b-amiRNA Lines.png|right|thumb|320px|'''Figure 2.''' ''Analyses of the MT-1-4b-amiRNA Lines. <ref name="ref1" />.'']]<br />
[[File:Figure 4. Molecular Identification of Rice MADS3..png|right|thumb|320px|'''Figure 3.''' ''Molecular Identification of Rice MADS3. <ref name="ref1" />'']]<br />
[[File:Figure 5. Rice MADS3 Expression Pattern Analysis..png|right|thumb|320px|'''Figure 4.''' Rice MADS3 Expression Pattern Analysis. <ref name="ref1" />'']]<br />
[[File:Figure 6. Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily.png|right|thumb|320px|'''Figure 5.''' Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily. <ref name="ref2" />'']]<br />
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'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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*'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.<br />
*'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.<br />
*'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.<br />
*The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br><br />
*mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. <br />
*mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
<br />
===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
<br />
==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170916Os01g02017002014-05-22T16:19:55Z<p>Ban Buzhu: </p>
<hr />
<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.(B) Panicle at the heading stage.(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .(G) and (H) Pollen grains at stage 12 stained by I2-KI.(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.<br />
MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]<br />
<br />
[[File:Figure 3. Analyses of the MT-1-4b-amiRNA Lines.png|right|thumb|320px|'''Figure 2.''' ''Analyses of the MT-1-4b-amiRNA Lines. <ref name="ref1" />.'']]<br />
[[File:Figure 4. Molecular Identification of Rice MADS3..png|right|thumb|320px|'''Figure 3.''' ''Molecular Identification of Rice MADS3. <ref name="ref1" />'']]<br />
[[File:Figure 5. Rice MADS3 Expression Pattern Analysis..png|right|thumb|320px|'''Figure 4.''' Rice MADS3 Expression Pattern Analysis. <ref name="ref1" />'']]<br />
[[File:Figure 6. Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily.png|right|thumb|320px|'''Figure 5.''' Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily. <ref name="ref2" />'']]<br />
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'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br><br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
<br />
===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
<br />
==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170915Os01g02017002014-05-22T16:17:14Z<p>Ban Buzhu: </p>
<hr />
<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
<br />
==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.(B) Panicle at the heading stage.(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .(G) and (H) Pollen grains at stage 12 stained by I2-KI.(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.<br />
MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]<br />
[[File:Figure 2. Analyses of ROS and Superoxide Anion levels in Wild-Type.png|right|thumb|320px|'''Figure 2.''' ''Analyses of ROS and Superoxide Anion levels in Wild-Type and mads3-4 Anthers. <ref name="ref2" />.<br>A) and (B) ROS (A) and superoxide anion (O2; [B]) levels. Data are presented as means 6 SE (n = 3).(C) and (D) NBT staining analysis of O2 production from early stages 8 to 12 showing the shallow to dark-blue colors.(E) and (F) O2 localization in the tapetum and microspores in tissue sections of anthers at stage 9. Bars = 5 mm. Msp, microspore; T, tapetum; WT, wild type. BS8, anthers before stage 8; S8-S12, anthers at stage 8 to 12.'']]<br />
[[File:Figure 3. Analyses of the MT-1-4b-amiRNA Lines.png|right|thumb|320px|'''Figure 3.''' ''Analyses of the MT-1-4b-amiRNA Lines. <ref name="ref1" />.'']]<br />
[[File:Figure 4. Molecular Identification of Rice MADS3..png|right|thumb|320px|'''Figure 4.''' ''Molecular Identification of Rice MADS3. <ref name="ref1" />'']]<br />
[[File:Figure 5. Rice MADS3 Expression Pattern Analysis..png|right|thumb|320px|'''Figure 5.''' Rice MADS3 Expression Pattern Analysis. <ref name="ref1" />'']]<br />
[[File:Figure 6. Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily.png|right|thumb|320px|'''Figure 6.''' Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily. <ref name="ref2" />'']]<br />
<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
<br />
'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br><br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
<br />
===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
<br />
===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
<br />
==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
<br />
*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
<br />
*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
<br />
==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170914Os01g02017002014-05-22T16:16:21Z<p>Ban Buzhu: </p>
<hr />
<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
<br />
==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.(B) Panicle at the heading stage.(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .(G) and (H) Pollen grains at stage 12 stained by I2-KI.(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.<br />
MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]<br />
[[File:Figure 2. Analyses of ROS and Superoxide Anion levels in Wild-Type.png|right|thumb|320px|'''Figure 2.''' ''Analyses of ROS and Superoxide Anion levels in Wild-Type and mads3-4 Anthers. <ref name="ref2" />.<br>A) and (B) ROS (A) and superoxide anion (O2; [B]) levels. Data are presented as means 6 SE (n = 3).(C) and (D) NBT staining analysis of O2 production from early stages 8 to 12 showing the shallow to dark-blue colors.(E) and (F) O2 localization in the tapetum and microspores in tissue sections of anthers at stage 9. Bars = 5 mm. Msp, microspore; T, tapetum; WT, wild type. BS8, anthers before stage 8; S8-S12, anthers at stage 8 to 12.'']]<br />
[[File:Figure 3. Analyses of the MT-1-4b-amiRNA Lines.png|right|thumb|320px|'''Figure 3.''' ''Analyses of the MT-1-4b-amiRNA Lines. <ref name="ref1" />.'']]<br />
[[File:Figure 4. Molecular Identification of Rice MADS3..png|right|thumb|320px|'''Figure 4.''' ''Molecular Identification of Rice MADS3. <ref name="ref1" />'']]<br />
[[File:Figure 5. Rice MADS3 Expression Pattern Analysis..png|right|thumb|320px|'''Figure 5.''' ' Rice MADS3 Expression Pattern Analysis. <ref name="ref1" />'']]<br />
[[File:Figure 6. Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily.png|right|thumb|320px|'''Figure 6.''' Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily. <ref name="ref2" />'']]<br />
<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
<br />
'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br><br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
<br />
===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
<br />
===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
<br />
===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
<br />
==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
<br />
*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
<br />
*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
<br />
==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170913Os01g02017002014-05-22T16:15:50Z<p>Ban Buzhu: </p>
<hr />
<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
<br />
==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.(B) Panicle at the heading stage.(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .(G) and (H) Pollen grains at stage 12 stained by I2-KI.(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.<br />
MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]<br />
[[File:Figure 2. Analyses of ROS and Superoxide Anion levels in Wild-Type.png|right|thumb|320px|'''Figure 2.''' ''Analyses of ROS and Superoxide Anion levels in Wild-Type and mads3-4 Anthers. <ref name="ref2" />.<br>A) and (B) ROS (A) and superoxide anion (O2; [B]) levels. Data are presented as means 6 SE (n = 3).(C) and (D) NBT staining analysis of O2 production from early stages 8 to 12 showing the shallow to dark-blue colors.(E) and (F) O2 localization in the tapetum and microspores in tissue sections of anthers at stage 9. Bars = 5 mm. Msp, microspore; T, tapetum; WT, wild type. BS8, anthers before stage 8; S8-S12, anthers at stage 8 to 12.'']]<br />
[[File:Figure 3. Analyses of the MT-1-4b-amiRNA Lines..png|right|thumb|320px|'''Figure 3.''' ''Analyses of the MT-1-4b-amiRNA Lines. <ref name="ref1" />.'']]<br />
[[File:Figure 4. Molecular Identification of Rice MADS3..png|right|thumb|320px|'''Figure 4.''' ''Molecular Identification of Rice MADS3. <ref name="ref1" />'']]<br />
[[File:Figure 5. Rice MADS3 Expression Pattern Analysis..png|right|thumb|320px|'''Figure 5.''' ' Rice MADS3 Expression Pattern Analysis. <ref name="ref1" />'']]<br />
[[File:Figure 6. Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily.png|right|thumb|320px|'''Figure 6.''' Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily. <ref name="ref2" />'']]<br />
<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
<br />
'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br><br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
<br />
===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
<br />
===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
<br />
===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
<br />
==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
<br />
*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
<br />
*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
<br />
==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170911Os01g02017002014-05-22T16:13:59Z<p>Ban Buzhu: </p>
<hr />
<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
<br />
==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.(B) Panicle at the heading stage.(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .(G) and (H) Pollen grains at stage 12 stained by I2-KI.(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.<br />
MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]<br />
[[File:Figure 2. Analyses of ROS and Superoxide Anion levels in Wild-Type.png|right|thumb|320px|'''Figure 2.''' ''Analyses of ROS and Superoxide Anion levels in Wild-Type and mads3-4 Anthers. <ref name="ref2" />.<br>A) and (B) ROS (A) and superoxide anion (O2; [B]) levels. Data are presented as means 6 SE (n = 3).(C) and (D) NBT staining analysis of O2 production from early stages 8 to 12 showing the shallow to dark-blue colors.(E) and (F) O2 localization in the tapetum and microspores in tissue sections of anthers at stage 9. Bars = 5 mm. Msp, microspore; T, tapetum; WT, wild type. BS8, anthers before stage 8; S8-S12, anthers at stage 8 to 12.'']]<br />
[[File:Figure 3. Analyses of the MT-1-4b-amiRNA Lines..png|right|thumb|320px|'''Figure 3.''' ''Analyses of the MT-1-4b-amiRNA Lines. <ref name="ref1" />.'']]<br />
[[File:Figure 4. Molecular Identification of Rice MADS3.png|right|thumb|320px|'''Figure 4.''' ''Molecular Identification of Rice MADS3. <ref name="ref1" />.'']]<br />
[[File:Figure 5. Rice MADS3 Expression Pattern Analysis..png|right|thumb|320px|'''Figure 5.''' ' Rice MADS3 Expression Pattern Analysis. <ref name="ref1" />.'']]<br />
[[File:Figure 6. Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily.png|right|thumb|320px|'''Figure 6.''' ' Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily. <ref name="ref2" />.'']]<br />
<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
<br />
'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br><br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
<br />
===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
<br />
===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
<br />
==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
<br />
*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
<br />
*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
<br />
==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170909Os01g02017002014-05-22T16:13:23Z<p>Ban Buzhu: </p>
<hr />
<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
<br />
==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.(B) Panicle at the heading stage.(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .(G) and (H) Pollen grains at stage 12 stained by I2-KI.(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.<br />
MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]<br />
[[File:Figure 2. Analyses of ROS and Superoxide Anion levels in Wild-Type.png|right|thumb|320px|'''Figure 2.''' ''Analyses of ROS and Superoxide Anion levels in Wild-Type and mads3-4 Anthers. <ref name="ref2" />.<br>A) and (B) ROS (A) and superoxide anion (O2; [B]) levels. Data are presented as means 6 SE (n = 3).(C) and (D) NBT staining analysis of O2 production from early stages 8 to 12 showing the shallow to dark-blue colors.(E) and (F) O2 localization in the tapetum and microspores in tissue sections of anthers at stage 9. Bars = 5 mm. Msp, microspore; T, tapetum; WT, wild type. BS8, anthers before stage 8; S8-S12, anthers at stage 8 to 12.'']]<br />
[[File:Figure 3. Analyses of the MT-1-4b-amiRNA Lines.png|right|thumb|320px|'''Figure 3.''' ''Analyses of the MT-1-4b-amiRNA Lines. <ref name="ref1" />.'']]<br />
[[File:Figure 4. Molecular Identification of Rice MADS3.png|right|thumb|320px|'''Figure 4.''' ''Molecular Identification of Rice MADS3. <ref name="ref1" />.'']]<br />
[[File:Figure 5. Rice MADS3 Expression Pattern Analysis.png|right|thumb|320px|'''Figure 5.''' ' Rice MADS3 Expression Pattern Analysis. <ref name="ref1" />.'']]<br />
[[File:Figure 6. Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily.png|right|thumb|320px|'''Figure 6.''' ' Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily. <ref name="ref2" />.'']]<br />
<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
<br />
'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br><br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
<br />
===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
<br />
===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
<br />
==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
<br />
*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
<br />
*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
<br />
==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170905Os01g02017002014-05-22T16:08:42Z<p>Ban Buzhu: </p>
<hr />
<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
<br />
==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.(B) Panicle at the heading stage.(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .(G) and (H) Pollen grains at stage 12 stained by I2-KI.(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.<br />
MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]<br />
[[File:Figure 2. Analyses of ROS and Superoxide Anion levels in Wild-Type.png|right|thumb|320px|'''Figure 2.''' ''Analyses of ROS and Superoxide Anion levels in Wild-Type and mads3-4 Anthers. <ref name="ref2" />.<br>A) and (B) ROS (A) and superoxide anion (O2; [B]) levels. Data are presented as means 6 SE (n = 3).(C) and (D) NBT staining analysis of O2 production from early stages 8 to 12 showing the shallow to dark-blue colors.(E) and (F) O2 localization in the tapetum and microspores in tissue sections of anthers at stage 9. Bars = 5 mm. Msp, microspore; T, tapetum; WT, wild type. BS8, anthers before stage 8; S8-S12, anthers at stage 8 to 12.'']]<br />
[[File:Figure 3. Analyses of the MT-1-4b-amiRNA Lines.png|right|thumb|320px|'''Figure 3.''' ''Analyses of the MT-1-4b-amiRNA Lines. <ref name="ref1" />.'']]<br />
<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br><br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
<br />
===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170902Os01g02017002014-05-22T16:07:44Z<p>Ban Buzhu: </p>
<hr />
<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.(B) Panicle at the heading stage.(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .(G) and (H) Pollen grains at stage 12 stained by I2-KI.(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.<br />
MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]<br />
[[File:Figure 2. Analyses of ROS and Superoxide Anion levels in Wild-Type.png|right|thumb|320px|'''Figure 2.''' ''Analyses of ROS and Superoxide Anion levels in Wild-Type and mads3-4 Anthers.(from reference) <ref name="ref2" />.<br>A) and (B) ROS (A) and superoxide anion (O2; [B]) levels. Data are presented as means 6 SE (n = 3).(C) and (D) NBT staining analysis of O2 production from early stages 8 to 12 showing the shallow to dark-blue colors.(E) and (F) O2 localization in the tapetum and microspores in tissue sections of anthers at stage 9. Bars = 5 mm. Msp, microspore; T, tapetum; WT, wild type. BS8, anthers before stage 8; S8-S12, anthers at stage 8 to 12.'']]<br />
[[File:Figure 3. Analyses of the MT-1-4b-amiRNA Lines..png|right|thumb|320px|'''Figure 3.''' ''Analyses of the MT-1-4b-amiRNA Lines.(from reference) <ref name="ref1" />.'']]<br />
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'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br><br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
<br />
===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170900Os01g02017002014-05-22T16:03:58Z<p>Ban Buzhu: </p>
<hr />
<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.(B) Panicle at the heading stage.(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .(G) and (H) Pollen grains at stage 12 stained by I2-KI.(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.<br />
MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]<br />
[[File:Figure 2. Analyses of ROS and Superoxide Anion levels in Wild-Type.png|right|thumb|320px|'''Figure 2.''' ''Analyses of ROS and Superoxide Anion levels in Wild-Type and mads3-4 Anthers.(from reference) <ref name="ref2" />.<br>A) and (B) ROS (A) and superoxide anion (O2; [B]) levels. Data are presented as means 6 SE (n = 3).(C) and (D) NBT staining analysis of O2� production from early stages 8 to 12 showing the shallow to dark-blue colors.(E) and (F) O2 localization in the tapetum and microspores in tissue sections of anthers at stage 9. Bars = 5 mm. Msp, microspore; T, tapetum; WT, wild type. BS8, anthers before stage 8; S8-S12, anthers at stage 8 to 12.'']]<br />
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'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br><br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
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===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170898Os01g02017002014-05-22T16:03:21Z<p>Ban Buzhu: </p>
<hr />
<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.(B) Panicle at the heading stage.(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .(G) and (H) Pollen grains at stage 12 stained by I2-KI.(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.<br />
MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]<br />
[[File:Figure 2. Analyses of ROS and Superoxide Anion levels in Wild-Type .png|right|thumb|320px|'''Figure 2.''' ''Analyses of ROS and Superoxide Anion levels in Wild-Type and mads3-4 Anthers.(from reference) <ref name="ref2" />.<br>A) and (B) ROS (A) and superoxide anion (O2; [B]) levels. Data are presented as means 6 SE (n = 3).(C) and (D) NBT staining analysis of O2� production from early stages 8 to 12 showing the shallow to dark-blue colors.(E) and (F) O2 localization in the tapetum and microspores in tissue sections of anthers at stage 9. Bars = 5 mm. Msp, microspore; T, tapetum; WT, wild type. BS8, anthers before stage 8; S8-S12, anthers at stage 8 to 12.'']]<br />
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'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br><br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
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===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170897Os01g02017002014-05-22T16:02:51Z<p>Ban Buzhu: </p>
<hr />
<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.(B) Panicle at the heading stage.(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .(G) and (H) Pollen grains at stage 12 stained by I2-KI.(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.<br />
MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]<br />
[[File:Figure 2. Analyses of ROS and Superoxide Anion levels in Wild-Type and mads3-4 Anthers..png|right|thumb|320px|'''Figure 2.''' ''Analyses of ROS and Superoxide Anion levels in Wild-Type and mads3-4 Anthers.(from reference) <ref name="ref2" />.<br>A) and (B) ROS (A) and superoxide anion (O2; [B]) levels. Data are presented as means 6 SE (n = 3).(C) and (D) NBT staining analysis of O2� production from early stages 8 to 12 showing the shallow to dark-blue colors.(E) and (F) O2 localization in the tapetum and microspores in tissue sections of anthers at stage 9. Bars = 5 mm. Msp, microspore; T, tapetum; WT, wild type. BS8, anthers before stage 8; S8-S12, anthers at stage 8 to 12.'']]<br />
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'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br><br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
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===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170894Os01g02017002014-05-22T15:58:31Z<p>Ban Buzhu: </p>
<hr />
<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.<br />
(B) Panicle at the heading stage.<br />
(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .<br />
(G) and (H) Pollen grains at stage 12 stained by I2-KI.<br />
(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.<br />
MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]<br />
<br />
<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
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===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170891Os01g02017002014-05-22T15:52:29Z<p>Ban Buzhu: </p>
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<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
[[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref4" />.<br>(C and D) shows a Complementation test. Cd concentration in the shoots (C) and roots (D) of three independent transgenic lines carrying ''OsHMA3'' and empty vector in Anjana Dhan background. (E and F) shows the Effect of decreased expression of '''OsHMA3''' on Cd accumulation. Cd concentration in the shoots (E) and roots (F) of three independent RNAi lines and vector control plants in Nipponbare background.'']]<br />
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'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
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===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170887Os01g02017002014-05-22T15:46:37Z<p>Ban Buzhu: </p>
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<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.[[File:Figure 1. Phenotypic Analysis of mads3-4..png]]'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
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===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170864Os01g02017002014-05-22T15:05:00Z<p>Ban Buzhu: </p>
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<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
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===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170863Os01g02017002014-05-22T15:01:22Z<p>Ban Buzhu: </p>
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<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.[[File:Figure 1. Phenotypic Analysis of mads3-4]]'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
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===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170858Os01g02017002014-05-22T14:52:18Z<p>Ban Buzhu: </p>
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<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.[[File:Figure 1. Phenotypic Analysis of mads3-4.]]'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
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===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Figure_6._Phylogenetic_Analysis_of_MADS_Domain_Proteins_in_the_AG_Subfamily.png&diff=170857File:Figure 6. Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily.png2014-05-22T14:50:43Z<p>Ban Buzhu: </p>
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<div></div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Figure_5._Rice_MADS3_Expression_Pattern_Analysis..png&diff=170856File:Figure 5. Rice MADS3 Expression Pattern Analysis..png2014-05-22T14:50:28Z<p>Ban Buzhu: </p>
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<div></div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Figure_4._Molecular_Identification_of_Rice_MADS3..png&diff=170854File:Figure 4. Molecular Identification of Rice MADS3..png2014-05-22T14:50:14Z<p>Ban Buzhu: </p>
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<div></div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Figure_3._Analyses_of_the_MT-1-4b-amiRNA_Lines.png&diff=170853File:Figure 3. Analyses of the MT-1-4b-amiRNA Lines.png2014-05-22T14:49:59Z<p>Ban Buzhu: </p>
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<div></div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Figure_2._Analyses_of_ROS_and_Superoxide_Anion_levels_in_Wild-Type.png&diff=170852File:Figure 2. Analyses of ROS and Superoxide Anion levels in Wild-Type.png2014-05-22T14:49:32Z<p>Ban Buzhu: </p>
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<div></div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Figure_1._Phenotypic_Analysis_of_mads3-4..png&diff=170851File:Figure 1. Phenotypic Analysis of mads3-4..png2014-05-22T14:47:12Z<p>Ban Buzhu: </p>
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<div></div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170850Os01g02017002014-05-22T14:45:28Z<p>Ban Buzhu: </p>
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<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
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===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS boy<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170849Os01g02017002014-05-22T14:43:04Z<p>Ban Buzhu: </p>
<hr />
<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
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===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS box[<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170847Os01g02017002014-05-22T14:41:34Z<p>Ban Buzhu: </p>
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<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
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===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS box[<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300]<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170845Os01g02017002014-05-22T14:39:49Z<p>Ban Buzhu: /* Annotated Information */</p>
<hr />
<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
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===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS box[<ref name="ref2" />.<br />
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===Knowledge Extension===<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170843Os01g02017002014-05-22T14:35:36Z<p>Ban Buzhu: </p>
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<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
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===Expression===<br />
the expression of '''OsMADS3'''is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of '''OsMADS3''' in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in '''OsMADS3''' expression was detected in anthers starting from stage 9, when young microspores form. The expression of '''OsMADS3''' peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the '''OsMADS3'''transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the '''OsMADS3''' promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS box[<ref name="ref2" />.<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170835Os01g02017002014-05-22T14:26:27Z<p>Ban Buzhu: </p>
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<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of OSMADS3 result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
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===Expression===<br />
the expression of MADS3 is detectable in stamen primordia<ref name="ref2" />, when the lemma and palea primordia initiate, but disappears soon after the appearance of the stamen primordia. However, mads3-4 seems to show strong defects in late anther development (Figures 1C to 1F). RT-PCR detected no obvious expression of MADS3 in vegetative organs, nonreproductive floral organs (i.e., glumes, lemma, and palea) (Figure 5A). By contrast, an increase in MADS3 expression was detected in anthers starting from stage 9, when young microspores form. The expression of MADS3 peaked at stage 11, when the bicellular pollen forms, and decreased at stage 12 (Figure 5A). In addition, the MADS3 transcript was weakly detected in the pistil at the heading stage (Figure 5A). Consistent with the RT-PCR data, transgenic rice plants expressing the GUS gene driven by the MADS3 promoter (3.1 kb) exhibited GUS activity from stage 9 to 12 during anther development (Figure 5B). GUS activity was also observed in the stigma of the pistil (Figure 5C). Furthermore, transverse anther sections showed GUS expression<ref name="ref1" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS box[<ref name="ref2" />.<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170823Os01g02017002014-05-22T13:52:17Z<p>Ban Buzhu: </p>
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<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of OSMADS3 result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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'''OsMADS3''' plays a synergistic role with '''OsMADS13''' in both ovule development and floral meristem termination<ref name="ref3" />.'''OsMADS3''' represses the expression of the putative A-class gene DEP ('''OsMADS15''')<ref name="ref3" />.'''OsMADS3''' and '''OsMADS58''' have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of '''OsMADS3''' and '''OsMADS58'''can be activated by '''OsMADS6''' to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
'''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.<br />
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===Expression===<br />
Expression of '''OsMADS3''' was first detected at the time when the lemma and palea primordia were just initiating (Figure 2A). At this stage, strong expression of '''OsMADS3''' was observed mainly in whorl 3, where the stamen primordia subsequently initiate, and only a weak signal was detected in whorl 4 (Figure 2A). Soon after initiation of the stamen primordia, the '''OsMADS3''' signal disappeared from the developing stamen primordia, but weak expression remained in whorl 4 (Figure 2B). Expression of '''OsMADS3''' was highly elevated in whorl 4 just before carpel development initiated (Figure 2C), but it disappeared from the carpel primordia after emergence of the carpel bulge (Figure 2D). At this stage, strong expression of '''OsMADS3''' was observed in the floral meristem, which later produces the ovule primordia (Figure 2D), but it was down regulated once the ovule primordia initiated (Figure 2E)<ref name="ref2" />.<br />
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===Evolution===<br />
'''OsMADS3''' is most closely related to '''OsMADS58''' among the rice MADS box genes. '''OsMADS3''' and '''OsMADS58''' share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS box[<ref name="ref2" />.<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170822Os01g02017002014-05-22T13:45:33Z<p>Ban Buzhu: </p>
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<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of OSMADS3 result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.MADS3 is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />. <br />
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OsMADS3 plays a synergistic role with OsMADS13 in both ovule development and floral meristem termination<ref name="ref3" />.OsMADS3 represses the expression of the putative A-class gene DEP (OsMADS15)<ref name="ref3" />.OSMADS3 and OSMADS58 have been subfunctionalized such that they play more predominant roles in distinct whorls<ref name="ref2" />.The expression of MADS3 and MADS58 can be activated by MADS6 to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[<ref name="ref4" />.<br />
===Mutation===<br />
MADS3 alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of MADS3, seems to be another intermediate mutant with reduced expression of MADS3 <ref name="ref1" />.<br />
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===Expression===<br />
Expression of OSMADS3 was first detected at the time when the lemma and palea primordia were just initiating (Figure 2A). At this stage, strong expression of OSMADS3 was observed mainly in whorl 3, where the stamen primordia subsequently initiate, and only a weak signal was detected in whorl 4 (Figure 2A). Soon after initiation of the stamen primordia, the OSMADS3 signal disappeared from the developing stamen primordia, but weak expression remained in whorl 4 (Figure 2B). Expression ofOSMADS3 was highly elevated in whorl 4 just before carpel development initiated (Figure 2C), but it disappeared from the carpel primordia after emergence of the carpel bulge (Figure 2D). At this stage, strong expression of OSMADS3 was observed in the floral meristem, which later produces the ovule primordia (Figure 2D), but it was down regulated once the ovule primordia initiated (Figure 2E)<ref name="ref2" />.<br />
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===Evolution===<br />
OSMADS3 is most closely related to OSMADS58 among the rice MADS box genes. OSMADS3 and OSMADS58 share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS box[<ref name="ref2" />.<br />
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==Labs working on this gene==<br />
*School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
<br />
*Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
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*Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
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*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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*Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
<references><br />
<ref name="ref1"><br />
Hu L, Liang W, Yin C, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. The Plant Cell Online, 2011, 23(2): 515-533.</ref><br />
<ref name="ref2"><br />
Yamaguchi T, Lee D Y, Miyao A, et al. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa[J]. The Plant Cell Online, 2006, 18(1): 15-28.</ref><br />
<ref name="ref3"><br />
Li, H.F., Liang, W.Q., Yin, C.S., Zhu, L., and Zhang, D.B. (2011).Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.Plant Physiol. 156: 263–274.</ref><br />
<ref name="ref4"><br />
Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170809Os01g02017002014-05-22T13:00:04Z<p>Ban Buzhu: </p>
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<div>The rice '''OsMADS3'''(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants<ref name="ref1" /><ref name="ref2" />.<br />
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==Annotated Information==<br />
===Function===<br />
'''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of OSMADS3 result severe defects in stamen identity and lodicule number[1][2].MADS3 is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b[1]. <br />
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OsMADS3 plays a synergistic role with OsMADS13 in both ovule development and floral meristem termination[3].OsMADS3 represses the expression of the putative A-class gene DEP (OsMADS15)[3].OSMADS3 and OSMADS58 have been subfunctionalized such that they play more predominant roles in distinct whorls[2].The expression of MADS3 and MADS58 can be activated by MADS6 to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[4].<br />
===Mutation===<br />
MADS3 alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules[2]. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs [2]. mads3-4, which results in a mutation in the middle region of the K-domain of MADS3, seems to be another intermediate mutant with reduced expression of MADS3 [1].<br />
<br />
===Expression===<br />
Expression of OSMADS3 was first detected at the time when the lemma and palea primordia were just initiating (Figure 2A). At this stage, strong expression of OSMADS3 was observed mainly in whorl 3, where the stamen primordia subsequently initiate, and only a weak signal was detected in whorl 4 (Figure 2A). Soon after initiation of the stamen primordia, the OSMADS3 signal disappeared from the developing stamen primordia, but weak expression remained in whorl 4 (Figure 2B). Expression ofOSMADS3 was highly elevated in whorl 4 just before carpel development initiated (Figure 2C), but it disappeared from the carpel primordia after emergence of the carpel bulge (Figure 2D). At this stage, strong expression of OSMADS3 was observed in the floral meristem, which later produces the ovule primordia (Figure 2D), but it was down regulated once the ovule primordia initiated (Figure 2E)[2].<br />
<br />
===Evolution===<br />
OSMADS3 is most closely related to OSMADS58 among the rice MADS box genes. OSMADS3 and OSMADS58 share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS box[2].<br />
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==Labs working on this gene==<br />
(1)School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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(2)Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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(3)Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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(4)Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
<br />
(5)b Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
<br />
(6) National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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(7) Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170808Os01g02017002014-05-22T12:48:00Z<p>Ban Buzhu: </p>
<hr />
<div>The rice OsMADS3(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants[1][2].<br />
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==Annotated Information==<br />
===Function===<br />
OsMADS3(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of OSMADS3 result severe defects in stamen identity and lodicule number[1][2].MADS3 is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b[1]. <br />
OsMADS3 plays a synergistic role with OsMADS13 in both ovule development and floral meristem termination[3].OsMADS3 represses the expression of the putative A-class gene DEP (OsMADS15)[3].OSMADS3 and OSMADS58 have been subfunctionalized such that they play more predominant roles in distinct whorls[2].The expression of MADS3 and MADS58 can be activated by MADS6 to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[4].<br />
===Mutation===<br />
MADS3 alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules[2]. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs [2]. mads3-4, which results in a mutation in the middle region of the K-domain of MADS3, seems to be another intermediate mutant with reduced expression of MADS3 [1].<br />
<br />
===Expression===<br />
Expression of OSMADS3 was first detected at the time when the lemma and palea primordia were just initiating (Figure 2A). At this stage, strong expression of OSMADS3 was observed mainly in whorl 3, where the stamen primordia subsequently initiate, and only a weak signal was detected in whorl 4 (Figure 2A). Soon after initiation of the stamen primordia, the OSMADS3 signal disappeared from the developing stamen primordia, but weak expression remained in whorl 4 (Figure 2B). Expression ofOSMADS3 was highly elevated in whorl 4 just before carpel development initiated (Figure 2C), but it disappeared from the carpel primordia after emergence of the carpel bulge (Figure 2D). At this stage, strong expression of OSMADS3 was observed in the floral meristem, which later produces the ovule primordia (Figure 2D), but it was down regulated once the ovule primordia initiated (Figure 2E)[2].<br />
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===Evolution===<br />
OSMADS3 is most closely related to OSMADS58 among the rice MADS box genes. OSMADS3 and OSMADS58 share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS box[2].<br />
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==Labs working on this gene==<br />
(1)School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
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(2)Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
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(3)Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
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(4)Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
<br />
(5)b Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
<br />
(6) National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
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(7) Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
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==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170807Os01g02017002014-05-22T12:47:17Z<p>Ban Buzhu: </p>
<hr />
<div>The rice OsMADS3(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants[1][2].<br />
<br />
==Annotated Information==<br />
===Function===<br />
OsMADS3(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of OSMADS3 result severe defects in stamen identity and lodicule number[1][2].MADS3 is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b[1]. <br />
OsMADS3 plays a synergistic role with OsMADS13 in both ovule development and floral meristem termination[3].OsMADS3 represses the expression of the putative A-class gene DEP (OsMADS15)[3].OSMADS3 and OSMADS58 have been subfunctionalized such that they play more predominant roles in distinct whorls[2].The expression of MADS3 and MADS58 can be activated by MADS6 to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[4].<br />
===Mutation===<br />
MADS3 alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules[2]. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs [2]. mads3-4, which results in a mutation in the middle region of the K-domain of MADS3, seems to be another intermediate mutant with reduced expression of MADS3 [1].<br />
<br />
===Expression===<br />
Expression of OSMADS3 was first detected at the time when the lemma and palea primordia were just initiating (Figure 2A). At this stage, strong expression of OSMADS3 was observed mainly in whorl 3, where the stamen primordia subsequently initiate, and only a weak signal was detected in whorl 4 (Figure 2A). Soon after initiation of the stamen primordia, the OSMADS3 signal disappeared from the developing stamen primordia, but weak expression remained in whorl 4 (Figure 2B). Expression ofOSMADS3 was highly elevated in whorl 4 just before carpel development initiated (Figure 2C), but it disappeared from the carpel primordia after emergence of the carpel bulge (Figure 2D). At this stage, strong expression of OSMADS3 was observed in the floral meristem, which later produces the ovule primordia (Figure 2D), but it was down regulated once the ovule primordia initiated (Figure 2E)[2].<br />
<br />
===Evolution===<br />
OSMADS3 is most closely related to OSMADS58 among the rice MADS box genes. OSMADS3 and OSMADS58 share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS box[2].<br />
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==Labs working on this gene==<br />
(1)School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
(2)Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
(3)Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
(4)Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
(5)b Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
(6) National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
(7) Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
<br />
==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170806Os01g02017002014-05-22T12:46:52Z<p>Ban Buzhu: /* Function */</p>
<hr />
<div>The rice OsMADS3(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants[1][2].<br />
<br />
==Annotated Information==<br />
===Function===<br />
<br />
===Mutation===<br />
MADS3 alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules[2]. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs [2]. mads3-4, which results in a mutation in the middle region of the K-domain of MADS3, seems to be another intermediate mutant with reduced expression of MADS3 [1].<br />
<br />
===Expression===<br />
Expression of OSMADS3 was first detected at the time when the lemma and palea primordia were just initiating (Figure 2A). At this stage, strong expression of OSMADS3 was observed mainly in whorl 3, where the stamen primordia subsequently initiate, and only a weak signal was detected in whorl 4 (Figure 2A). Soon after initiation of the stamen primordia, the OSMADS3 signal disappeared from the developing stamen primordia, but weak expression remained in whorl 4 (Figure 2B). Expression ofOSMADS3 was highly elevated in whorl 4 just before carpel development initiated (Figure 2C), but it disappeared from the carpel primordia after emergence of the carpel bulge (Figure 2D). At this stage, strong expression of OSMADS3 was observed in the floral meristem, which later produces the ovule primordia (Figure 2D), but it was down regulated once the ovule primordia initiated (Figure 2E)[2].<br />
<br />
===Evolution===<br />
OSMADS3 is most closely related to OSMADS58 among the rice MADS box genes. OSMADS3 and OSMADS58 share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS box[2].<br />
<br />
<br />
==Labs working on this gene==<br />
(1)School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
(2)Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
(3)Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
(4)Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
(5)b Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
(6) National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
(7) Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
<br />
==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170805Os01g02017002014-05-22T12:46:27Z<p>Ban Buzhu: </p>
<hr />
<div>The rice OsMADS3(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants[1][2].<br />
<br />
==Annotated Information==<br />
===Function===<br />
OsMADS3(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of OSMADS3 result severe defects in stamen identity and lodicule number[1][2].MADS3 is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b[1]. figure 1.<br />
OsMADS3 plays a synergistic role with OsMADS13 in both ovule development and floral meristem termination[3].OsMADS3 represses the expression of the putative A-class gene DEP (OsMADS15)[3].OSMADS3 and OSMADS58 have been subfunctionalized such that they play more predominant roles in distinct whorls[2].The expression of MADS3 and MADS58 can be activated by MADS6 to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[4].<br />
===Mutation===<br />
MADS3 alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules[2]. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs [2]. mads3-4, which results in a mutation in the middle region of the K-domain of MADS3, seems to be another intermediate mutant with reduced expression of MADS3 [1].<br />
<br />
===Expression===<br />
Expression of OSMADS3 was first detected at the time when the lemma and palea primordia were just initiating (Figure 2A). At this stage, strong expression of OSMADS3 was observed mainly in whorl 3, where the stamen primordia subsequently initiate, and only a weak signal was detected in whorl 4 (Figure 2A). Soon after initiation of the stamen primordia, the OSMADS3 signal disappeared from the developing stamen primordia, but weak expression remained in whorl 4 (Figure 2B). Expression ofOSMADS3 was highly elevated in whorl 4 just before carpel development initiated (Figure 2C), but it disappeared from the carpel primordia after emergence of the carpel bulge (Figure 2D). At this stage, strong expression of OSMADS3 was observed in the floral meristem, which later produces the ovule primordia (Figure 2D), but it was down regulated once the ovule primordia initiated (Figure 2E)[2].<br />
<br />
===Evolution===<br />
OSMADS3 is most closely related to OSMADS58 among the rice MADS box genes. OSMADS3 and OSMADS58 share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS box[2].<br />
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==Labs working on this gene==<br />
(1)School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
(2)Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
(3)Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
(4)Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
(5)b Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
(6) National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
(7) Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
<br />
==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170804Os01g02017002014-05-22T12:45:52Z<p>Ban Buzhu: /* Function */</p>
<hr />
<div>The rice OsMADS3(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants[1][2].<br />
<br />
==Annotated Information==<br />
===Function===<br />
<br />
===Mutation===<br />
MADS3 alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules[2]. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs [2]. mads3-4, which results in a mutation in the middle region of the K-domain of MADS3, seems to be another intermediate mutant with reduced expression of MADS3 [1].<br />
<br />
===Expression===<br />
Expression of OSMADS3 was first detected at the time when the lemma and palea primordia were just initiating (Figure 2A). At this stage, strong expression of OSMADS3 was observed mainly in whorl 3, where the stamen primordia subsequently initiate, and only a weak signal was detected in whorl 4 (Figure 2A). Soon after initiation of the stamen primordia, the OSMADS3 signal disappeared from the developing stamen primordia, but weak expression remained in whorl 4 (Figure 2B). Expression ofOSMADS3 was highly elevated in whorl 4 just before carpel development initiated (Figure 2C), but it disappeared from the carpel primordia after emergence of the carpel bulge (Figure 2D). At this stage, strong expression of OSMADS3 was observed in the floral meristem, which later produces the ovule primordia (Figure 2D), but it was down regulated once the ovule primordia initiated (Figure 2E)[2].<br />
<br />
===Evolution===<br />
OSMADS3 is most closely related to OSMADS58 among the rice MADS box genes. OSMADS3 and OSMADS58 share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS box[2].<br />
<br />
<br />
==Labs working on this gene==<br />
(1)School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
(2)Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
(3)Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
(4)Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
(5)b Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
(6) National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
(7) Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
<br />
==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170803Os01g02017002014-05-22T12:45:36Z<p>Ban Buzhu: </p>
<hr />
<div>The rice OsMADS3(Os01g0201700) is a member of MADS-box transcription factors in rice, it belongs to class C genes of the ABC model of flower development in plants[1][2].<br />
<br />
==Annotated Information==<br />
===Function===<br />
OsMADS3 plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of OSMADS3 result severe defects in stamen identity and lodicule number[1][2].MADS3 is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b[1]. figure 1.<br />
OsMADS3 plays a synergistic role with OsMADS13 in both ovule development and floral meristem termination[3].OsMADS3 represses the expression of the putative A-class gene DEP (OsMADS15)[3].OSMADS3 and OSMADS58 have been subfunctionalized such that they play more predominant roles in distinct whorls[2].The expression of MADS3 and MADS58 can be activated by MADS6 to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[4].<br />
<br />
===Mutation===<br />
MADS3 alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br />
mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules[2]. mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs [2]. mads3-4, which results in a mutation in the middle region of the K-domain of MADS3, seems to be another intermediate mutant with reduced expression of MADS3 [1].<br />
<br />
===Expression===<br />
Expression of OSMADS3 was first detected at the time when the lemma and palea primordia were just initiating (Figure 2A). At this stage, strong expression of OSMADS3 was observed mainly in whorl 3, where the stamen primordia subsequently initiate, and only a weak signal was detected in whorl 4 (Figure 2A). Soon after initiation of the stamen primordia, the OSMADS3 signal disappeared from the developing stamen primordia, but weak expression remained in whorl 4 (Figure 2B). Expression ofOSMADS3 was highly elevated in whorl 4 just before carpel development initiated (Figure 2C), but it disappeared from the carpel primordia after emergence of the carpel bulge (Figure 2D). At this stage, strong expression of OSMADS3 was observed in the floral meristem, which later produces the ovule primordia (Figure 2D), but it was down regulated once the ovule primordia initiated (Figure 2E)[2].<br />
<br />
===Evolution===<br />
OSMADS3 is most closely related to OSMADS58 among the rice MADS box genes. OSMADS3 and OSMADS58 share 96 and 67% sequence identity in the MADS domain and across the whole protein, respectively.OSMADS3 and OSMADS58 share similar genomic organization: they contain 10 exons and 9 introns at the same positions and have a large intron after the second exon, which partly contains the MADS box[2].<br />
<br />
<br />
==Labs working on this gene==<br />
(1)School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<br />
(2)Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of Education,Shanghai Jiao Tong University, Shanghai 200240, China<br />
(3)Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824<br />
(4)Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<br />
(5)b Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea<br />
(6) National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan<br />
(7) Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br />
<br />
==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170802Os01g02017002014-05-22T12:40:10Z<p>Ban Buzhu: /* Function */</p>
<hr />
<div>Please input one-sentence summary here.<br />
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==Annotated Information==<br />
===Function===<br />
OsMADS3 plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of OSMADS3 result severe defects in stamen identity and lodicule number[1][2].MADS3 is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b[1]. figure 1.<br />
OsMADS3 plays a synergistic role with OsMADS13 in both ovule development and floral meristem termination[3].OsMADS3 represses the expression of the putative A-class gene DEP (OsMADS15)[3].OSMADS3 and OSMADS58 have been subfunctionalized such that they play more predominant roles in distinct whorls[2].The expression of MADS3 and MADS58 can be activated by MADS6 to regulates the stamen, carpel, and meristem identities at early stages and specifies carpel/ovule development[4].<br />
<br />
===Mutation===<br />
Please input expression information here.<br />
<br />
===Expression===<br />
Please input expression information here.<br />
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===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
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==References==<br />
Please input cited references here.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170721Os01g02017002014-05-22T09:15:58Z<p>Ban Buzhu: /* Annotated Information */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Please input expression information here.<br />
<br />
===Mutation===<br />
Please input expression information here.<br />
<br />
===Expression===<br />
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<br />
===Evolution===<br />
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<br />
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==Labs working on this gene==<br />
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==References==<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170720Os01g02017002014-05-22T09:15:31Z<p>Ban Buzhu: /* Annotated Information */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Please input expression information here.<br />
<br />
===Function===<br />
Please input expression information here.<br />
<br />
===Expression===<br />
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<br />
===Evolution===<br />
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==Labs working on this gene==<br />
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==References==<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170719Os01g02017002014-05-22T09:14:12Z<p>Ban Buzhu: /* Annotated Information */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Please input expression information here.<br />
<br />
===Expression===<br />
Please input expression information here.<br />
<br />
===Evolution===<br />
Please input evolution information here.<br />
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==Labs working on this gene==<br />
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==References==<br />
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<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhuhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0201700&diff=170717Os01g02017002014-05-22T09:13:29Z<p>Ban Buzhu: /* Mutation */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
<br />
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<br />
===Expression===<br />
Please input expression information here.<br />
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===Evolution===<br />
Please input evolution information here.<br />
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==Labs working on this gene==<br />
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==References==<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0201700|<br />
Description = Similar to MADS box protein|<br />
Version = NM_001185293.1 GI:297719720 GeneID:9271572|<br />
Length = 9393 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0201700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:5558512..5567904|<br />
CDS = 5560361..5560379,5560446..5560507,5561618..5561699,5567047..5567333,5567665..5567679<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:5558512..5567904<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgatgaacatgatgaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacagtctaactcggcttgcctaa</cdnaseq>|<br />
AA = <aaseq>MMNMMTDLSCGPSSMTELTAAAAPAGSGSSAAVAAGSSEKMGRG KIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSV KSTVERYKKANSDTSNSGTVAEVNAQHYQQESSKLRQQISSLQNANSLTRLA</aaseq>|<br />
DNA = <dnaseqindica>7526..7544#7398..7459#6206..6287#572..858#226..240#atcagattggaattggccacaaaagctactgctaactacattcacctcacatttcttcttcccctttccctcctcctcctcttcttcctcttcttctcccccacctctctctttaatacctctcctcctcctgttgctgcttctgcctctctcacatcttctttctttccttctctggatctcttccatcttcagaagagagagcactagttagcatacccatccatgatgaacatgatggtaaattctggctttcttgcttttggataaattttgcttcctttcttaacttgagcacaagcttgtgttatatgtggtgtggaatcttggttgccatgttgtgaggatttagctagagagtcaagaaagaggaatatatgctttatgtagataggagtaggatctctgggtctttaaacatcaccatgacaagcaaagataagaacaggagagcagttcttgattattatttttcttctcatcaagaaattaagccggagatagacatggcagctgcacgcagtgattcacttcttgatttcttgatttgggttgttgcgtttgtgtccagaccgatctgagctgcgggccatcgtcgatgacggagctgaccgcggcagcggcgccggctgggtcaggatcgtcggcggcggtggcggcggggagcagcgagaagatggggagggggaagatcgagataaagcggatcgagaacacgacgaaccggcaggtgaccttctgcaagcgccgcaatggcctcctgaagaaggcgtacgagctgtccgtcctctgcgacgccgaggttgccctcatcgtcttctccagccgcggccgcctctacgagtacgccaacaacaggtgattcacaaaatactatctcatctcatcctacttatgcttatataatccataaaattaaccaagaggtctaaaaaggctatggaaatatgcatacacaatagaagctacacatatgtttcttttttttaaaaaaaaagaagaaaatgtttgttggttgagttcatggttgtccaaattggagcaagtatggagatagctttcatgggggcaaagagaggggagggtcaaatgggagatgcatcttttgttacttctgttattaactgtctcgtgttatttggtgtttctggggggaaagaaagaaaatcgcaacagtttctctcttgcttgcttgtgtgtgtgtgctagctagcttcttttgctgctttcctttgtgcttctggtgaggtgagctagatgtttgagatagatctaggacagtgcttgctgtgtgtagtatgctgctgcttcctttctttctctctctctctctctctctctcttgtgtgtgtgagatgagagagagagagagatgttcttctttggcttggtggtcctgctcttttcttgctgtatttatgttttttcctttctctctccactgtggcaagctgtgtgcttgtatgtctgtgtggcagagctagctgtagagatagatgaagagagagagagaggtgagctttaagatctctctcacacctcatgatgtgtatgatcacacacatatttgcacaaaaaatatagagcatcctggggctccattcatcatctttcccttttgtgtagctttgcttgtgtagaagcagatctctagatagcaaggagagtgtgtatggttggccatggcattgatgtactagtagttagtttgtacatgaatgtgccaagtgttagtgtatgtatgtttgatcaggtaataagggtagagggataaacaagcagctgatgatataacactctctctctctcttgtgttccagtttcttttgcatcatctcctgtttccaaccaatgagctgctagggttagggcttcatccccatgtgcatctctctctctctctctcatgagtgtgtgtggctgtgtggtgcaaattttagtcccgagcaacttcacgagggcaggtgatgcctgacagcaccactgtacttttgaacaggaaccaaacacaaagaagggatccaacaaagaaacagcagtaaagatgaaaaaaatacagtaaagtatataggggagtacatatgcatgcgtttgatttactgaaagaatttacacaaagatgattgttccatattttttgcatgtctccctttcctcataagctatacatactgaataatgtgattatataaaaatattagctagtctttttttacaaatgattttgttatgactattagttctagttatgtgtcgtatgcatgtaaataaaaaagtggcaggataagcttatttgttttttcatcaaataaagaaaactaaaatttgtgtaccaaaatatcagtttcaatgttatgtgaaaacatgtcatgcaggcataaaaaaaattacacacatggccactgttgtctagttatgttgaagaaaaaactgaaaaactgtggtctgcatgaagtatttgaaatcaattaataaaacatattgactttttcttttttgcttcgcgactaatcaatcctccttgaaaaacgaaattagaagcacatatatacgttttgttgcactgcttaaacatggggatcagaaacctctcatgtatcctcaccaaaaaaaaaaaaagagaaagaaaaagaagcctctcttgtacggatgacacgtgtccatgcagccactgtcgcagtagaaatggcgtccaatggcgtggcagcaatggtacaactactgggcctgcatttgtattgggagactacaagaggagtgaagaaaagctggtggctttggtcagaatgggaatgggtaaaagcctacagttgtgtagtgtagtctctgtgtgtgcgttgctgccttgttgccctcgtttgtccattgggttaaaaccagtctcactgttgctgcccaatcatgtgaaatcctgtgcccagcttcacattgggcagccaaatagcgttctcgagaatcctaaggtttcatttctttcttgccaggaagatagtaggattgatgtctgtacttcagtactaccattactccaatttctttggtcctcgaacaatatgtttactggtacattaccactgtaatgtgacatcttttttgcatttttttagataatggacatcttttttgctacccaaaatataagaaattctaaggaatgacagaaagtaggtgaaataaaataggaaataattgtggtaggttgatattgaggagcaggtgaaaaaagtttttatatttaggatggagggattaagtaatttcttgcctgaaaatcttcatttgggttggaggggattgatagtgctcgctgcttgatatgattgtttgttagttattttatgcagcaattgcacgctttatgtttggacaaaggaaaatttcaaaggttgtagtgaaaagacatgagctagctagcagtcacttcacagtagcatgcatgaacagaagatctttgaagaatatttttggagaaccttttggggatatctttggtatacagtgatctctagctaggttttttttagggtagtactccgtatactatttccacatatttgtcggtccttggaaattaaaatgatttattctcatggatttatcatcataagcagtttctgctcaagtattgtcagcagtttgtgttattgacttgtttggtagttttttttttttttgaagaacttatttgctagttggctgactaggtagcaagtacaccattttggaaggtggccttccacaattttctgtctaaatatgcttaattatatggacataacaatatctcctttgtgacaataaaaactgtggaactacgtagttccacgtatacgttacgtggttacattagtaagtacaaccttgtaaaacattagcatgtcatatgcattgatctgcctaagaaacgagaataaaatctctgcgatgagaagtactgttggagagtttatagattgcttaaggtaataggatattttttgttttcttgttatatactattcatcgtaagctctgctcctattgccgcatgccctgttttcttcaagctattcaagctagatatgatgctttgcttgataactatgcaagaaaaaacaagatttataaggtcaaaataagtttgcttcctacatgcttgggtttttcatatgttaaggttgaaaatcataattaacaaacacaccaaaactaaaatagaatacataatgtgatcgtgaggtgaacacgggataagtgttgtttgggctgtcaaacgtacgttctatacaactgaactccaaatatatattatcatccataccatcatttttgagttttcttatttttcaaaaaagagaaaatactagtgtatccccagtccatagtatatctaagccttgatttttttaccttttatttatagtattattagagttcacttaagaacttagctctggtactactcatttttttatactaaatttatgaatgcactttgtaagtcatcacatatttgtacatctaattgtttagcaatgtgcacaaaattaactatttgttatctagttaaggtgtatattaatatgattccgtgatttcgttaacttgtactgaaaacggagtagcatgttagggccactattctatagctttatctgttaattcgaacctgcaatttcagaagtgtctttatatcggtttcaaagagtgataaagcgcacatacactgatccttagtccattatatgtagtcttgtatgctctttttcggatgaaaatagttttatacgcttggtgccattattctcttgacatagtgtctcaaaattttgtaacttaatgctgatctttagttcattatatgtagtcttatatgctctttttcggatgaaaatagttttatacgcttgctaccattattctctcttgacgtagtctctcaaaattccgaacttcacagtctcagtttgcatactttttgttttctcttctttgatttagggagccttgagttattttcgttgaatagtacgtattactagtatataagaagcacatagcagcgacttttgatttgcagtcattttataatatgaaaaataccccacaaaaaataatatgaaaaatagaataagtgaaactagtaataatcggcaagtgctaactaatggggataaatataactcatgaaatattgccaaataagatgatgtctgaatacactggagtcgcacaattgtttttttccttgtctggtggcgtcagctagcgtttgtgttcacttgtcccttcgacacaccggtatatcacgatttcttgtgccgtacccaaaaacaaaatcatggataagaaacacacatcacaccaatggagtcgtctagtctcattttgtgcatcaaaacccccagatcgatgacaggaaaagctctctatctcctcggtaaagagaaacagtactccactgtgctactctgcagtagttcatgaatgagcgagtgacttgtgatagcgcacgattcggaccaatgtgctatgttgtgttttaactatggtcatttgcccttgaacgccacattctaaatgtgaagtattccctcctgttcatatgataaatcgtttgatttttttttctaatcaaacttctttaagtttattaaattatagaaaaatatattagtatgttcaacataaaacaaaaatattatcaaaataattaatgtttaatttaatgaaactaatttgatattttagatgttaaatttttttataaacttgatcaaactaacaaagtttgactagaaaagaaaatcaaatgacttataatatgaaatggagggaatactagatattaatgtcagtttcaactttctactgcacttatttatactgtattctctgtattactaattgatgttggttattttcgtccttttagtagtacatgaagatgctgacaagctatgctcagttgcctcttgtgtaaacttttaaaaccaatacttatacgataatttcgggatagtaaattcaattaattatcatacttttctccctcatagctagcttgccgaaaattatatgttctagaaaaaaaaacttttttggaattgcctagcgagtaagttttttttttcgcagtcgtcttggctatactgttctgaaactggtaatttcttgtgtaggagtgattaaacataacccttgactaatcatggtcatattgcagtgtgaaatccaccgttgagaggtacaagaaggcaaacagtgacacctccaactctggcacagttgcagaagtcaatgcccaggtaacatcatgtcatttctttcagtctacattcttcaactagtactaaattttccgaattgactccgagatgtggacatatatagatatcagaataacagtaagagcaatctttaaatgctatagaactttcaacattcttactagttactgttgtgcaaacgcaaacatgcatgacctctctgagcaaaatcttcagacaagaaatcctgagcagaaagttatgggcattacacagcggttattgcagccaaccaatgctctgaggagtcaggatcctctgtatgatccagtggatcacatgcaattgttcattagaccaatatcgtaatttcatggcggtagagatgcctaggggttcacaaatcttagacaaacattgaaaatttgtggattaatgtagagaacaattaaaaccatcaattgtttatgcttagtcatgtccaccagccatgaattaatcttgcaatttatgagtatcataaaccagctagtagtcaaaaactgcagtatgtatccccttgaaggcttgaacagaactatttggtgtgctcagtatattataggttgaaggaaactataggggtgtgaagtagtatttctaataagaattaatttcctggcaccacatgaaatattactccataaacaaatgtactacgtaagtagttagtagtagtaaaaacagactatctgaatttatacactaactccatcctatttcttaacttatataacttaatttgatgattctatcccagcacagtaagctactatagtatgaaccttcagactgcactgatgtttttctgctagagtggtgtattgcatacagtagatttttattgaaaaaaaaacttggcctgtaaaatttcttatagctaaatctgagagatcatgtagttttttgtgaagaatatgacgtacgtaacataaaacttttaaacaaagacatgtttaatacataagtgaaaattgaaatagactaattataacccaaacaggagtttttctctgcgaatgttcggttgaacatcatttcagctagaatatgttttcaacataaactttatgtactgatgaatgctatcatatatgctgcaaactgcagcactaccagcaggagtcctccaaactgcgccaacaaatcagtagcttacagaacgcaaacaggtaaagattccagatacgtatgacttacttttgcaccaatataaaccaaaatgttctgtattgcagtctaactcggcttgcctaaatatctctagtaggaccatagtgggggattctatcaacaccatgagcctcagggaccttaaacaggtagagaacaggctggagaaaggcatagctaagataagggctagaaaggtatatagtgcaactcaatttaaagcattatataacttatagtatacatcagctatttcttcatgacttttgactaagtgcaaattctatgttctttaattccatgcatgagctatcgatgtcctttgtatatctcaatttcattttgatcgtcctaatcaacccagtggacatttctttatcgcagaatgagctgttatatgctgaagttgagtacatgcagaaaagggtaagtaagatcatagcacgagaactcgttttatgccaaccgtacgtagtttcctttcttttgcatgtcttagttccatttttcagaccaaattacatgtcacatatatattattcttcaggaagttgagctgcagaatgacaacatgtacctgaggagcaaggtaaatacattcctatgaggataagttattgcagttccaatatagttaattaccaactgaattctttctgagagctgtggcctctgattatataaataattgattatgtaggttgttgagaatgagaggggacagcagccactgaacatgatgggggcagcatcaacaagtgaatacgatcatatggttaataacccatatgattccaggaactttcttcaagtgaacatcatgcagcagcctcagcattacgcccatcagctgcagccaactacccttcaactcgggtaaattgagatcaattaactgaatatatatgcttacagaatttcatgctccagagatcgagattatattattcagtctttttgtgtttcttacaaatccagctacatatgttaatatacagttcttgtttaacttttgtttctattctgcaggcagcagccggccttcaattagttttggtgtagacaccgtacgtacacacgtacgttaggtaaataaaacactcagtactgtagatttatacctacacaaaagtatatatttgcagagtataatccagttaggcaagttgatatttaccctctgctatttttacataactgccagcggatactatttactttcgaactttggcaactcgtctttggaaatgataaaatgttattaaagtgtgctatatcttaaatacaagcatcggacttttgttcactttggtctaacaatttgagagaaaacaatgttcaatattcaaatggaaagtaaatgttgtcagttatttagaaacgaagggagtatatgtattactggcattcatcttaataattaactcagtaactgtttcttacatctcttgtagttgaaatctgaaagtgtgttgtatctgttgcagatgaaatctgaaggcgtcgatacccgcggctacgtaacagcgtgatcaacctgaagaaatcgatgcagtaattctatgtgttcgtcagatcatcataaccagtattgtcattaggcgttgtgaaaaaaaaatgtgtcagcatgcattgcaagctagcgtttgtggctgctcatcagctgcatgcgtaattgcgtacgtgcacaaattaaacagaccttgccaccattgtgccatattgtgtgtgttgtatgatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatataaacagcacgtttgtgctctgattcgtgtaactgatcagcagatcaaactctaccactgccgatatatatgtgaggaccatggattgatgcattgtcgttgatcttc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185293.1 RefSeq:Os01g0201700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Ban Buzhu