http://192.168.164.12:81/ricewiki/api.php?action=feedcontributions&feedformat=atom&user=DYM 2026-05-24T04:27:54Z User contributions MediaWiki 1.30.0 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184087 2014-06-14T07:08:55Z

<p>DYM: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig. 1.jpg|right|thumb|150px|'''Figure 1. Expression Profiles of Wda1 by GUS Assay and RT-PCR.''']]<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Fig. 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig 2.jpg|right|thumb|150px|'''Figure 2. Scheme of the Wda1 Gene, Multiple Alignments, and Phylogenic Analysis of WDA1-Related Proteins.''' ]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> &lt;references&gt; <br /> &lt;ref name=&quot;ref1&quot;&gt;Ki-Hong Jung,Min-Jung Han,Dong-yeun Lee,et al.Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development.The Plant Cell,2006,18(11):3015-3032.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0471100|<br /> Description = Sterol desaturase family protein|<br /> Version = NM_001071361.1 GI:115482465 GeneID:4348869|<br /> Length = 5861 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0471100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:17906207..17912067|<br /> CDS = 17906494..17906655,17906746..17906922,17907019..17907117,17907202..17907402,17907731..17907838&lt;br&gt;,17907926..17908307,17908870..17909089,17910772..17911004,17911137..17911360&lt;br&gt;,17911825..17911884|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactgggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccattatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATNPGLFTEWPWKKLGSFKYVLLAPWVAHGWYEVATKGWREVD LGYIAILPSLLLRMLHNQAWITISRLQNARGRRQIVRRGIEFDQVDRERNWDDQIILS GILLYLGALYVPGGQHLPLWRTDGAGLIALLHAGPVEFLYYWFHRALHHHFLYTHYHS HHHSSIVTEPITSVIHPFAELVAYELLFSIPLIACALTGTASIIAFEMYLIYIDFMNN MGHCNFELVPSWLFTWFPPLKYLMYTPSFHSLHHTQFRTNYSLFMPFYDYIYNTMDKS SDTLYENSLKNNEEEEAVDVVHLTHLTTLHSIYHMRPGFAEFASRPYVSRWYMRMMWP LSWLSMVLTWTYGSSFTVERNVMKKIRMQSWAIPRYSFHYGLDWEKEAINDLIEKAVC EADKNGAKVVSLGLLNQAHTLNKSGEQYLLKYPKLGARIVDGTSLAAAVVVNSIPQGT DQVILAGNVSKVARAVAQALCKKNIKVTMTNKQDYHLLKPEIPETVADNLSFSKTGTA KVWLIGDGLDSAEQFRAQKGTLFIPYSQFPPKMVRKDSCSYSTTPAMAVPKTLQNVHS CENWLPRRVMSAWRIAGILHALEGWNEHECGDKVLDMDKVWSAAIMHGFCPVAQG&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5413..5574#5146..5322#4951..5049#4666..4866#4230..4337#3761..4142#2979..3198#1064..1296#708..931#184..243#atatgagactctgacacgtacgcacagctagatcgaagccctggtcgagtttataaattgagatatatccgtcctccattctcaggccagttcttgcaagactcttgccgtgttgagactgcagaaacgagagctagctagccagaggctgcaagaagattctgcacccatagatcgtctaccatggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaaggtaatcaaaattaaccttccatcttcttctccttaattcagttacttcactgtcttctggcagctcgatcgagcaaataaacgcacagcttctagttccatccatggcagcactgcaccatgaccattatgttcttgtttgagttcgctagcttctgtttgatatcaaagcatacattgccgcatgtgcagctagcagtgcatgagattaattctcttgcatctgctgcagatcagtaatgcaggggcatgaactttcctgcagcacctctgtgcagcaaataaactgttaaagagtacatggttaaataagcttttgccacatcgtctttgattaggcaaaaacattcagtatacacaacttccgacctttacaaattacaacagtaacttcagctataaattatagcacatgtgagttaattcgatggatggattcatgcatgtatatgtgtgcatatgcagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactggtatgccaaacaccataaaattacgtacaacccacgcggtgtgttcgtggcggctagcttcaccttcgagattaattaattcaacctcattatagttgatgtgttgttacgtgtatggtggtgtgcgtgcagggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatgtacgtaattaattgccactagagacggtttatcctcgaggctccaaacattacattctcttttaggcaataacctttgagcttctatattagtatcgaattgtgttgcgtcataaatatttttacaaaatttttattcatcatctaaagttatcaatgatcaaagttcaatcaatactaatagttacaagtaatttgaaatagaggaagtaagttgtactacctagttgtaccatcattgctccaaacaacctattacatgatattactttcgtcccaaacctctaaatgaatattaagattttggtaatacaaattcttaaaattttgagacagaaatattactagacctgtaaccttgggataaattaaaccacggtaatcttccaatagaacaaagtaaaaaacaagtcttttccataaggacacaaaacaggaggtgtacttcgtcagatgcaagtgtcgcgcgtgtccgttcctcaggaaacacagagtgttcctgtggccagtcgatcgctacttcaattccctcctccttcaatgcatgcctgaacaataattgtaggcctagagagtgtgtgagttaaggaatgcaaaggacacacgatatttcatggaccattgagatcgaagtaagccgaccgatcgatcgcgagctaaatttatgtagcccatcgaatccgttgctgaatgcctgaccaaacggagaagcaaacgtggtccatgaacaacgtatcagagacttttagacaagagagaaatcacattctaaggggttttttcccccttttttttgagtggaagagttttttttattgagaggtatcgagaagtactatatttttcgtgtaagctagccaaatatcagtgcgttgcaacttgcaacggattattaaaaatagcaaaattaagtccttgttaaattttttttaaaaaaaaatggttgaaacacaacttttcatcttaaagaaacaaacaaaaatgccatgcttagaaaattaaagagctaaagtatagtacagtatatatagtacaggcgactctcccaaaccttaataccatggcccatatatcagtgacccaggccctgggcaaactggtccacggcccagggcctgagatgaaaggcccgatgagaaatatgcatttttactaggaataagtctatttttcctcctttcaaccatgtaactttgtgttgggccaaaatttccatggggcggcctaggccttggcctatttctgggtccgccccttatcagtgattgtggtgatggcagattcactaccaccactactatcttttaatagtagtaaagattttggtgtcatcttgtaattttaagatgtacaagacggtaccataaattgactttaaagtgatttgaatggatgtgtaatgggagaaatcgcttttcaaatgtaaaataatgcactacttgtattagtactaaattttacattagagaacacgatatattcagtgattcttgataaccataaaatccctttaagattatggtgttttctttaagtactgttaaaaaaaaaccaatagtgtctactggttacactgcagaagaagagaaccctgcagactgcagtatttgtaattgagcaagcagatacatatacctaaatgcatatatgttcattacctacaattcctaacgttgttgcagccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtaagtacttcatcaggctcttaataatacttccttcttccttaaatgtttgacgccgttgacttttttaaacatgtttgaccgttcgtcttattcaaaaacttttgtgaaatgtgtaaaactatatgtatacataaaagtatatttaacaataaatcaaatgatagaaaaagaattaataattatttaaatttttgaataagacgaacggtcaaatatttttaaaaaagtcaatggcatcaaatattttgggatggagggagtatgtaaaagagtaactttacagtttttaagaaagcataggggaagtaccatattttatattggagaaattttacggttttcagtatttcttttaagataaaggaggctttattgatcttggatacaatatactctaaaaccactcctgatatctgcatagctaggatgcacatggtctatagttattgaaaaagccaaataaaagaagatgatgtgcatgtcactctgcaaaataaataacttgatcatacatgctgttttcactgttataataatctgaacttatgtgatatattaggtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccatgtaagagtaaccatgttcagtgcatgattcacagatggtgtgcatgataaattgataatcaggttagaaacattttgttttgtgcagtatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggtagctcatttcctaatgaattcagagcaagttgaaacattatcatccatacttcagaattcagatgttagctatatgtccacatgtactgccaaattttagtcaagataaaacagttagtgcttaataattgtttatcttcagggaaaaggtttagtcataaaggtaggaaaataaaaaaattaagtagcaaagagtccgttgacagtagtccaacagaactaataaattctgtacatttcatgagaatacaatatgtagaaattagggaagtatgtactcatgcatggttgaactggtaattgacatacaatatcttcaatataaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtaattaatccactactcacctctgatttattttatagcatgcaaaaggataccatgttgtaacaaaccactattcttgtacaggtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtaagaatagatttcctcatttttcctacattctctggacaaatgaatagaatgtataagagaaatatcattgcctaataaaccaaaaattttcaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgaggtagtagtaataacctgttgaaataatccagagattcagacgaacttcacatgatctaatcacaaaatttttgaactttttttttgacagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttgatgattcagttgcaaagtatcagaataagttttagctggattatgctatgtggtcaccgtatcagaaagagagatgttctgttggcaagaagagatatagtatctgagtaatttcatctgccatggtttttaaaacattgttcatcatctcctttgatcaaagactggccatggctactttctgtataagtatctgtcctttttagtccatagtgtaattcagcaacaaaacactcttggcattgttatgcagtctcatgaatgtttttccagcatacggaattacgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071361.1 RefSeq:Os10g0471100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184086 2014-06-14T07:06:25Z

<p>DYM: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig. 1.jpg|right|thumb|150px|'''Figure 1. Expression Profiles of Wda1 by GUS Assay and RT-PCR.''']]<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Fig. 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig 2.jpg|right|thumb|150px|'''Figure 2. Scheme of the Wda1 Gene, Multiple Alignments, and Phylogenic Analysis of WDA1-Related Proteins.''' ]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> &lt;references&gt; <br /> &lt;ref name=&quot;ref1&quot;&gt;Ki-Hong Jung,Min-Jung Han,Dong-yeun Lee,et al.Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development.The Plant Cell,2006,18(11):3015-3032.</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184085 2014-06-14T07:05:55Z

<p>DYM: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig. 1.jpg|right|thumb|150px|'''Figure 1. Expression Profiles of Wda1 by GUS Assay and RT-PCR.''']]<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Fig. 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig 2.jpg|right|thumb|150px|'''Figure 2. Scheme of the Wda1 Gene, Multiple Alignments, and Phylogenic Analysis of WDA1-Related Proteins.''' ]]<br /> <br /> ===Structured Information===<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> &lt;references&gt; <br /> &lt;ref name=&quot;ref1&quot;&gt;Ki-Hong Jung,Min-Jung Han,Dong-yeun Lee,et al.Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development.The Plant Cell,2006,18(11):3015-3032.</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184084 2014-06-14T07:05:26Z

<p>DYM: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig. 1.jpg|right|thumb|150px|'''Figure 1. Expression Profiles of Wda1 by GUS Assay and RT-PCR.''']]<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Fig. 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig 2.jpg|right|thumb|150px|'''Figure 2. Scheme of the Wda1 Gene, Multiple Alignments, and Phylogenic Analysis of WDA1-Related Proteins.''' ]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> &lt;references&gt; <br /> &lt;ref name=&quot;ref1&quot;&gt;Ki-Hong Jung,Min-Jung Han,Dong-yeun Lee,et al.Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development.The Plant Cell,2006,18(11):3015-3032.</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184083 2014-06-14T07:05:13Z

<p>DYM: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig. 1.jpg|right|thumb|150px|'''Figure 1. Expression Profiles of Wda1 by GUS Assay and RT-PCR.''']]<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Fig. 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig 2.jpg|right|thumb|150px|'''Figure 2. Scheme of the Wda1 Gene, Multiple Alignments, and Phylogenic Analysis of WDA1-Related Proteins.''' ]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> &lt;references&gt; <br /> &lt;ref name=&quot;ref1&quot;&gt;Ki-Hong Jung,Min-Jung Han,Dong-yeun Lee,et al.Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development.The Plant Cell,2006,18(11):3015-3032.<br /> <br /> ==Structured Information==</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184082 2014-06-14T07:04:37Z

<p>DYM: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig. 1.jpg|right|thumb|150px|'''Figure 1. Expression Profiles of Wda1 by GUS Assay and RT-PCR.''']]<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Fig. 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig 2.jpg|right|thumb|150px|'''Figure 2. Scheme of the Wda1 Gene, Multiple Alignments, and Phylogenic Analysis of WDA1-Related Proteins.''' ]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> &lt;references&gt; <br /> &lt;ref name=&quot;ref1&quot;&gt;Ki-Hong Jung,Min-Jung Han,Dong-yeun Lee,et al.Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development.The Plant Cell,2006,18(11):3015-3032.<br /> <br /> ===Structured Information===</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184081 2014-06-14T07:04:16Z

<p>DYM: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig. 1.jpg|right|thumb|150px|'''Figure 1. Expression Profiles of Wda1 by GUS Assay and RT-PCR.''']]<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Fig. 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig 2.jpg|right|thumb|150px|'''Figure 2. Scheme of the Wda1 Gene, Multiple Alignments, and Phylogenic Analysis of WDA1-Related Proteins.''' ]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> &lt;references&gt; <br /> &lt;ref name=&quot;ref1&quot;&gt;Ki-Hong Jung,Min-Jung Han,Dong-yeun Lee,et al.Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development.The Plant Cell,2006,18(11):3015-3032.<br /> <br /> ==Structured Information==</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184080 2014-06-14T07:01:15Z

<p>DYM: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig. 1.jpg|right|thumb|150px|'''Figure 1. Expression Profiles of Wda1 by GUS Assay and RT-PCR.''']]<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Fig. 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig 2.jpg|right|thumb|150px|'''Figure 2. Scheme of the Wda1 Gene, Multiple Alignments, and Phylogenic Analysis of WDA1-Related Proteins.''' ]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> &lt;references&gt; <br /> &lt;ref name=&quot;ref1&quot;&gt;Ki-Hong Jung,Min-Jung Han,Dong-yeun Lee,et al.Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development.The Plant Cell,2006,18(11):3015-3032.<br /> <br /> ==Structured Information==<br /> ===Function===<br /> ===Expression===<br /> ===Evolution===</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184079 2014-06-14T06:59:36Z

<p>DYM: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig. 1.jpg|right|thumb|150px|'''Figure 1. Expression Profiles of Wda1 by GUS Assay and RT-PCR.''']]<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Fig. 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig 2.jpg|right|thumb|150px|'''Figure 2. Scheme of the Wda1 Gene, Multiple Alignments, and Phylogenic Analysis of WDA1-Related Proteins.''' ]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> &lt;references&gt; <br /> &lt;ref name=&quot;ref1&quot;&gt;Ki-Hong Jung,Min-Jung Han,Dong-yeun Lee,et al.Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development.The Plant Cell,2006,18(11):3015-3032.<br /> <br /> ==Structured Information==<br /> ==Annotated Information==<br /> ===Function===<br /> ===Expression===<br /> ===Evolution===</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184078 2014-06-14T06:56:49Z

<p>DYM: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig. 1.jpg|right|thumb|150px|'''Figure 1. Expression Profiles of Wda1 by GUS Assay and RT-PCR.''']]<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Fig. 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig 2.jpg|right|thumb|150px|'''Figure 2. Scheme of the Wda1 Gene, Multiple Alignments, and Phylogenic Analysis of WDA1-Related Proteins.''' ]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> &lt;references&gt; <br /> &lt;ref name=&quot;ref1&quot;&gt;Ki-Hong Jung,Min-Jung Han,Dong-yeun Lee,et al.Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development.The Plant Cell,2006,18(11):3015-3032.<br /> <br /> ==Structured Information==</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184077 2014-06-14T06:56:33Z

<p>DYM: /* Structured Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig. 1.jpg|right|thumb|150px|'''Figure 1. Expression Profiles of Wda1 by GUS Assay and RT-PCR.''']]<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Fig. 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig 2.jpg|right|thumb|150px|'''Figure 2. Scheme of the Wda1 Gene, Multiple Alignments, and Phylogenic Analysis of WDA1-Related Proteins.''' ]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184076 2014-06-14T06:55:37Z

<p>DYM: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig. 1.jpg|right|thumb|150px|'''Figure 1. Expression Profiles of Wda1 by GUS Assay and RT-PCR.''']]<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Fig. 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig 2.jpg|right|thumb|150px|'''Figure 2. Scheme of the Wda1 Gene, Multiple Alignments, and Phylogenic Analysis of WDA1-Related Proteins.''' ]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> &lt;references&gt; <br /> &lt;ref name=&quot;ref1&quot;&gt;Ki-Hong Jung,Min-Jung Han,Dong-yeun Lee,et al.Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development.The Plant Cell,2006,18(11):3015-3032.</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Fig_2.jpg&diff=184075 2014-06-14T06:52:44Z

<p>DYM: uploaded a new version of &amp;quot;File:Fig 2.jpg&amp;quot;</p> <hr /> <div></div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184074 2014-06-14T06:50:20Z

<p>DYM: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig. 1.jpg|right|thumb|150px|'''Figure 1. Expression Profiles of Wda1 by GUS Assay and RT-PCR.''']]<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Figure 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> &lt;references&gt; <br /> &lt;ref name=&quot;ref1&quot;&gt;Ki-Hong Jung,Min-Jung Han,Dong-yeun Lee,et al.Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development.The Plant Cell,2006,18(11):3015-3032.</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184073 2014-06-14T06:49:21Z

<p>DYM: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs&lt;ref name=&quot;ref1&quot; /&gt;.<br /> [[File:Fig. 1.jpg|right|thumb|150px|&quot;Figure 1. Expression Profiles of Wda1 by GUS Assay and RT-PCR.&quot;]]<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Figure 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> &lt;references&gt; <br /> &lt;ref name=&quot;ref1&quot;&gt;Ki-Hong Jung,Min-Jung Han,Dong-yeun Lee,et al.Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development.The Plant Cell,2006,18(11):3015-3032.</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Fig._1.jpg&diff=184072 2014-06-14T06:43:17Z

<p>DYM: uploaded a new version of &amp;quot;File:Fig. 1.jpg&amp;quot;</p> <hr /> <div>Fig. 1 Distribution of CysR10 and CysP13 in PB-Is of developing rice endosperm at 3 WAF &lt;ref name=&quot;ref2&quot; /&gt;. (D) The distribution of CysR10 as visualized using anti-CysR10 and rhodamine-conjugated secondary antibodies. (E) The localization of CysP13 using anti-CysP13 and FITC-conjugated secondary antibodies. (F) Merged image of D and E. Bars in (D–F): 10mm.</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184071 2014-06-14T06:33:52Z

<p>DYM: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Figure 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> &lt;references&gt; <br /> &lt;ref name=&quot;ref1&quot;&gt;Ki-Hong Jung,Min-Jung Han,Dong-yeun Lee,et al.Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development.The Plant Cell,2006,18(11):3015-3032.</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184070 2014-06-14T06:33:41Z

<p>DYM: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Figure 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> &lt;references&gt; <br /> &lt;ref name=&quot;ref1&quot;&gt;Ki-Hong Jung,Min-Jung Han,Dong-yeun Lee,et al.Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development.The Plant Cell,2006,18(11):3015-3032.</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184069 2014-06-14T06:31:38Z

<p>DYM: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs.<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Figure 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> &lt;references&gt; <br /> &lt;ref name=&quot;ref1&quot;&gt;Ki-Hong Jung,Min-Jung Han,Dong-yeun Lee,et al.Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development.The Plant Cell,2006,18(11):3015-3032.</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184068 2014-06-14T06:29:58Z

<p>DYM: /* Structured Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs.<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Figure 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> &lt;references&gt; <br /> &lt;ref name=&quot;ref1&quot;&gt;Ki-Hong Jung,Min-Jung Han,Dong-yeun Lee,et al.Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development.The Plant Cell,2006,18(11):3015-3032.</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184067 2014-06-14T06:29:23Z

<p>DYM: /* Structured Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs.<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Figure 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0471100|<br /> Description = Sterol desaturase family protein|<br /> Version = NM_001071361.1 GI:115482465 GeneID:4348869|<br /> Length = 5861 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0471100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:17906207..17912067|<br /> CDS = 17906494..17906655,17906746..17906922,17907019..17907117,17907202..17907402,17907731..17907838&lt;br&gt;,17907926..17908307,17908870..17909089,17910772..17911004,17911137..17911360&lt;br&gt;,17911825..17911884|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactgggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccattatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATNPGLFTEWPWKKLGSFKYVLLAPWVAHGWYEVATKGWREVD LGYIAILPSLLLRMLHNQAWITISRLQNARGRRQIVRRGIEFDQVDRERNWDDQIILS GILLYLGALYVPGGQHLPLWRTDGAGLIALLHAGPVEFLYYWFHRALHHHFLYTHYHS HHHSSIVTEPITSVIHPFAELVAYELLFSIPLIACALTGTASIIAFEMYLIYIDFMNN MGHCNFELVPSWLFTWFPPLKYLMYTPSFHSLHHTQFRTNYSLFMPFYDYIYNTMDKS SDTLYENSLKNNEEEEAVDVVHLTHLTTLHSIYHMRPGFAEFASRPYVSRWYMRMMWP LSWLSMVLTWTYGSSFTVERNVMKKIRMQSWAIPRYSFHYGLDWEKEAINDLIEKAVC EADKNGAKVVSLGLLNQAHTLNKSGEQYLLKYPKLGARIVDGTSLAAAVVVNSIPQGT DQVILAGNVSKVARAVAQALCKKNIKVTMTNKQDYHLLKPEIPETVADNLSFSKTGTA KVWLIGDGLDSAEQFRAQKGTLFIPYSQFPPKMVRKDSCSYSTTPAMAVPKTLQNVHS CENWLPRRVMSAWRIAGILHALEGWNEHECGDKVLDMDKVWSAAIMHGFCPVAQG&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5413..5574#5146..5322#4951..5049#4666..4866#4230..4337#3761..4142#2979..3198#1064..1296#708..931#184..243#atatgagactctgacacgtacgcacagctagatcgaagccctggtcgagtttataaattgagatatatccgtcctccattctcaggccagttcttgcaagactcttgccgtgttgagactgcagaaacgagagctagctagccagaggctgcaagaagattctgcacccatagatcgtctaccatggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaaggtaatcaaaattaaccttccatcttcttctccttaattcagttacttcactgtcttctggcagctcgatcgagcaaataaacgcacagcttctagttccatccatggcagcactgcaccatgaccattatgttcttgtttgagttcgctagcttctgtttgatatcaaagcatacattgccgcatgtgcagctagcagtgcatgagattaattctcttgcatctgctgcagatcagtaatgcaggggcatgaactttcctgcagcacctctgtgcagcaaataaactgttaaagagtacatggttaaataagcttttgccacatcgtctttgattaggcaaaaacattcagtatacacaacttccgacctttacaaattacaacagtaacttcagctataaattatagcacatgtgagttaattcgatggatggattcatgcatgtatatgtgtgcatatgcagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactggtatgccaaacaccataaaattacgtacaacccacgcggtgtgttcgtggcggctagcttcaccttcgagattaattaattcaacctcattatagttgatgtgttgttacgtgtatggtggtgtgcgtgcagggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatgtacgtaattaattgccactagagacggtttatcctcgaggctccaaacattacattctcttttaggcaataacctttgagcttctatattagtatcgaattgtgttgcgtcataaatatttttacaaaatttttattcatcatctaaagttatcaatgatcaaagttcaatcaatactaatagttacaagtaatttgaaatagaggaagtaagttgtactacctagttgtaccatcattgctccaaacaacctattacatgatattactttcgtcccaaacctctaaatgaatattaagattttggtaatacaaattcttaaaattttgagacagaaatattactagacctgtaaccttgggataaattaaaccacggtaatcttccaatagaacaaagtaaaaaacaagtcttttccataaggacacaaaacaggaggtgtacttcgtcagatgcaagtgtcgcgcgtgtccgttcctcaggaaacacagagtgttcctgtggccagtcgatcgctacttcaattccctcctccttcaatgcatgcctgaacaataattgtaggcctagagagtgtgtgagttaaggaatgcaaaggacacacgatatttcatggaccattgagatcgaagtaagccgaccgatcgatcgcgagctaaatttatgtagcccatcgaatccgttgctgaatgcctgaccaaacggagaagcaaacgtggtccatgaacaacgtatcagagacttttagacaagagagaaatcacattctaaggggttttttcccccttttttttgagtggaagagttttttttattgagaggtatcgagaagtactatatttttcgtgtaagctagccaaatatcagtgcgttgcaacttgcaacggattattaaaaatagcaaaattaagtccttgttaaattttttttaaaaaaaaatggttgaaacacaacttttcatcttaaagaaacaaacaaaaatgccatgcttagaaaattaaagagctaaagtatagtacagtatatatagtacaggcgactctcccaaaccttaataccatggcccatatatcagtgacccaggccctgggcaaactggtccacggcccagggcctgagatgaaaggcccgatgagaaatatgcatttttactaggaataagtctatttttcctcctttcaaccatgtaactttgtgttgggccaaaatttccatggggcggcctaggccttggcctatttctgggtccgccccttatcagtgattgtggtgatggcagattcactaccaccactactatcttttaatagtagtaaagattttggtgtcatcttgtaattttaagatgtacaagacggtaccataaattgactttaaagtgatttgaatggatgtgtaatgggagaaatcgcttttcaaatgtaaaataatgcactacttgtattagtactaaattttacattagagaacacgatatattcagtgattcttgataaccataaaatccctttaagattatggtgttttctttaagtactgttaaaaaaaaaccaatagtgtctactggttacactgcagaagaagagaaccctgcagactgcagtatttgtaattgagcaagcagatacatatacctaaatgcatatatgttcattacctacaattcctaacgttgttgcagccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtaagtacttcatcaggctcttaataatacttccttcttccttaaatgtttgacgccgttgacttttttaaacatgtttgaccgttcgtcttattcaaaaacttttgtgaaatgtgtaaaactatatgtatacataaaagtatatttaacaataaatcaaatgatagaaaaagaattaataattatttaaatttttgaataagacgaacggtcaaatatttttaaaaaagtcaatggcatcaaatattttgggatggagggagtatgtaaaagagtaactttacagtttttaagaaagcataggggaagtaccatattttatattggagaaattttacggttttcagtatttcttttaagataaaggaggctttattgatcttggatacaatatactctaaaaccactcctgatatctgcatagctaggatgcacatggtctatagttattgaaaaagccaaataaaagaagatgatgtgcatgtcactctgcaaaataaataacttgatcatacatgctgttttcactgttataataatctgaacttatgtgatatattaggtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccatgtaagagtaaccatgttcagtgcatgattcacagatggtgtgcatgataaattgataatcaggttagaaacattttgttttgtgcagtatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggtagctcatttcctaatgaattcagagcaagttgaaacattatcatccatacttcagaattcagatgttagctatatgtccacatgtactgccaaattttagtcaagataaaacagttagtgcttaataattgtttatcttcagggaaaaggtttagtcataaaggtaggaaaataaaaaaattaagtagcaaagagtccgttgacagtagtccaacagaactaataaattctgtacatttcatgagaatacaatatgtagaaattagggaagtatgtactcatgcatggttgaactggtaattgacatacaatatcttcaatataaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtaattaatccactactcacctctgatttattttatagcatgcaaaaggataccatgttgtaacaaaccactattcttgtacaggtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtaagaatagatttcctcatttttcctacattctctggacaaatgaatagaatgtataagagaaatatcattgcctaataaaccaaaaattttcaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgaggtagtagtaataacctgttgaaataatccagagattcagacgaacttcacatgatctaatcacaaaatttttgaactttttttttgacagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttgatgattcagttgcaaagtatcagaataagttttagctggattatgctatgtggtcaccgtatcagaaagagagatgttctgttggcaagaagagatatagtatctgagtaatttcatctgccatggtttttaaaacattgttcatcatctcctttgatcaaagactggccatggctactttctgtataagtatctgtcctttttagtccatagtgtaattcagcaacaaaacactcttggcattgttatgcagtctcatgaatgtttttccagcatacggaattacgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071361.1 RefSeq:Os10g0471100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]<br /> &lt;references&gt; <br /> &lt;ref name=&quot;ref1&quot;&gt;Ki-Hong Jung,Min-Jung Han,Dong-yeun Lee,et al.Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development.The Plant Cell,2006,18(11):3015-3032.</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184066 2014-06-14T06:13:55Z

<p>DYM: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs.<br /> <br /> ===Evolution===<br /> The Wda1 Gene derived from a T-DNA Insertional Mutation. The transcript of the wda1 gene is 2337 bp long, including an 1866-bp coding sequence. The locus name of this gene has been registered as OSJNBa0079L16.17 in GenBank (http://www.ncbi.nlm.nih.gov) and as LOC_Os10g33250 in The Institute for Genomic Research database (http://tigrblast.tigr.org/euk-blast/). The primary structure of the gene comprises 10 exons and 9 introns (Figure 2A ). The T-DNA insertion was located at 311 bp downstream from the ATG start codon, in the first intron of the gene. Multiple alignments of plant proteins showing high similarity with the WDA1 protein(Fig. 2B). WDA1 (top line) was aligned with three rice proteins (LOC_Os02g56920, LOC_Os02g40780, and Os Wax2-like [LOC_Os09g25850]) and four Arabidopsis proteins (CER1, CER1-like, At2g37700, and WAX2). Black background indicates amino acid residues that are &gt;70% conserved, and gray background indicates amino acids that are &gt;30% conserved. Asterisks indicate perfectly matched amino acids, colons indicate highly conserved amino acids, and dots indicate low-matched amino acids among these eight proteins. Phylogenic analysis of proteins similar to WDA1 suggests that WDA1 is likely involved in VLCFA metabolism.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0471100|<br /> Description = Sterol desaturase family protein|<br /> Version = NM_001071361.1 GI:115482465 GeneID:4348869|<br /> Length = 5861 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0471100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:17906207..17912067|<br /> CDS = 17906494..17906655,17906746..17906922,17907019..17907117,17907202..17907402,17907731..17907838&lt;br&gt;,17907926..17908307,17908870..17909089,17910772..17911004,17911137..17911360&lt;br&gt;,17911825..17911884|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactgggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccattatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATNPGLFTEWPWKKLGSFKYVLLAPWVAHGWYEVATKGWREVD LGYIAILPSLLLRMLHNQAWITISRLQNARGRRQIVRRGIEFDQVDRERNWDDQIILS GILLYLGALYVPGGQHLPLWRTDGAGLIALLHAGPVEFLYYWFHRALHHHFLYTHYHS HHHSSIVTEPITSVIHPFAELVAYELLFSIPLIACALTGTASIIAFEMYLIYIDFMNN MGHCNFELVPSWLFTWFPPLKYLMYTPSFHSLHHTQFRTNYSLFMPFYDYIYNTMDKS SDTLYENSLKNNEEEEAVDVVHLTHLTTLHSIYHMRPGFAEFASRPYVSRWYMRMMWP LSWLSMVLTWTYGSSFTVERNVMKKIRMQSWAIPRYSFHYGLDWEKEAINDLIEKAVC EADKNGAKVVSLGLLNQAHTLNKSGEQYLLKYPKLGARIVDGTSLAAAVVVNSIPQGT DQVILAGNVSKVARAVAQALCKKNIKVTMTNKQDYHLLKPEIPETVADNLSFSKTGTA KVWLIGDGLDSAEQFRAQKGTLFIPYSQFPPKMVRKDSCSYSTTPAMAVPKTLQNVHS CENWLPRRVMSAWRIAGILHALEGWNEHECGDKVLDMDKVWSAAIMHGFCPVAQG&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5413..5574#5146..5322#4951..5049#4666..4866#4230..4337#3761..4142#2979..3198#1064..1296#708..931#184..243#atatgagactctgacacgtacgcacagctagatcgaagccctggtcgagtttataaattgagatatatccgtcctccattctcaggccagttcttgcaagactcttgccgtgttgagactgcagaaacgagagctagctagccagaggctgcaagaagattctgcacccatagatcgtctaccatggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaaggtaatcaaaattaaccttccatcttcttctccttaattcagttacttcactgtcttctggcagctcgatcgagcaaataaacgcacagcttctagttccatccatggcagcactgcaccatgaccattatgttcttgtttgagttcgctagcttctgtttgatatcaaagcatacattgccgcatgtgcagctagcagtgcatgagattaattctcttgcatctgctgcagatcagtaatgcaggggcatgaactttcctgcagcacctctgtgcagcaaataaactgttaaagagtacatggttaaataagcttttgccacatcgtctttgattaggcaaaaacattcagtatacacaacttccgacctttacaaattacaacagtaacttcagctataaattatagcacatgtgagttaattcgatggatggattcatgcatgtatatgtgtgcatatgcagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactggtatgccaaacaccataaaattacgtacaacccacgcggtgtgttcgtggcggctagcttcaccttcgagattaattaattcaacctcattatagttgatgtgttgttacgtgtatggtggtgtgcgtgcagggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatgtacgtaattaattgccactagagacggtttatcctcgaggctccaaacattacattctcttttaggcaataacctttgagcttctatattagtatcgaattgtgttgcgtcataaatatttttacaaaatttttattcatcatctaaagttatcaatgatcaaagttcaatcaatactaatagttacaagtaatttgaaatagaggaagtaagttgtactacctagttgtaccatcattgctccaaacaacctattacatgatattactttcgtcccaaacctctaaatgaatattaagattttggtaatacaaattcttaaaattttgagacagaaatattactagacctgtaaccttgggataaattaaaccacggtaatcttccaatagaacaaagtaaaaaacaagtcttttccataaggacacaaaacaggaggtgtacttcgtcagatgcaagtgtcgcgcgtgtccgttcctcaggaaacacagagtgttcctgtggccagtcgatcgctacttcaattccctcctccttcaatgcatgcctgaacaataattgtaggcctagagagtgtgtgagttaaggaatgcaaaggacacacgatatttcatggaccattgagatcgaagtaagccgaccgatcgatcgcgagctaaatttatgtagcccatcgaatccgttgctgaatgcctgaccaaacggagaagcaaacgtggtccatgaacaacgtatcagagacttttagacaagagagaaatcacattctaaggggttttttcccccttttttttgagtggaagagttttttttattgagaggtatcgagaagtactatatttttcgtgtaagctagccaaatatcagtgcgttgcaacttgcaacggattattaaaaatagcaaaattaagtccttgttaaattttttttaaaaaaaaatggttgaaacacaacttttcatcttaaagaaacaaacaaaaatgccatgcttagaaaattaaagagctaaagtatagtacagtatatatagtacaggcgactctcccaaaccttaataccatggcccatatatcagtgacccaggccctgggcaaactggtccacggcccagggcctgagatgaaaggcccgatgagaaatatgcatttttactaggaataagtctatttttcctcctttcaaccatgtaactttgtgttgggccaaaatttccatggggcggcctaggccttggcctatttctgggtccgccccttatcagtgattgtggtgatggcagattcactaccaccactactatcttttaatagtagtaaagattttggtgtcatcttgtaattttaagatgtacaagacggtaccataaattgactttaaagtgatttgaatggatgtgtaatgggagaaatcgcttttcaaatgtaaaataatgcactacttgtattagtactaaattttacattagagaacacgatatattcagtgattcttgataaccataaaatccctttaagattatggtgttttctttaagtactgttaaaaaaaaaccaatagtgtctactggttacactgcagaagaagagaaccctgcagactgcagtatttgtaattgagcaagcagatacatatacctaaatgcatatatgttcattacctacaattcctaacgttgttgcagccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtaagtacttcatcaggctcttaataatacttccttcttccttaaatgtttgacgccgttgacttttttaaacatgtttgaccgttcgtcttattcaaaaacttttgtgaaatgtgtaaaactatatgtatacataaaagtatatttaacaataaatcaaatgatagaaaaagaattaataattatttaaatttttgaataagacgaacggtcaaatatttttaaaaaagtcaatggcatcaaatattttgggatggagggagtatgtaaaagagtaactttacagtttttaagaaagcataggggaagtaccatattttatattggagaaattttacggttttcagtatttcttttaagataaaggaggctttattgatcttggatacaatatactctaaaaccactcctgatatctgcatagctaggatgcacatggtctatagttattgaaaaagccaaataaaagaagatgatgtgcatgtcactctgcaaaataaataacttgatcatacatgctgttttcactgttataataatctgaacttatgtgatatattaggtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccatgtaagagtaaccatgttcagtgcatgattcacagatggtgtgcatgataaattgataatcaggttagaaacattttgttttgtgcagtatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggtagctcatttcctaatgaattcagagcaagttgaaacattatcatccatacttcagaattcagatgttagctatatgtccacatgtactgccaaattttagtcaagataaaacagttagtgcttaataattgtttatcttcagggaaaaggtttagtcataaaggtaggaaaataaaaaaattaagtagcaaagagtccgttgacagtagtccaacagaactaataaattctgtacatttcatgagaatacaatatgtagaaattagggaagtatgtactcatgcatggttgaactggtaattgacatacaatatcttcaatataaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtaattaatccactactcacctctgatttattttatagcatgcaaaaggataccatgttgtaacaaaccactattcttgtacaggtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtaagaatagatttcctcatttttcctacattctctggacaaatgaatagaatgtataagagaaatatcattgcctaataaaccaaaaattttcaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgaggtagtagtaataacctgttgaaataatccagagattcagacgaacttcacatgatctaatcacaaaatttttgaactttttttttgacagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttgatgattcagttgcaaagtatcagaataagttttagctggattatgctatgtggtcaccgtatcagaaagagagatgttctgttggcaagaagagatatagtatctgagtaatttcatctgccatggtttttaaaacattgttcatcatctcctttgatcaaagactggccatggctactttctgtataagtatctgtcctttttagtccatagtgtaattcagcaacaaaacactcttggcattgttatgcagtctcatgaatgtttttccagcatacggaattacgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071361.1 RefSeq:Os10g0471100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184065 2014-06-14T05:58:23Z

<p>DYM: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs.<br /> <br /> ===Evolution===<br /> Phylogenic analysis suggests that WDA1 is likely involved in VLCFA metabolism.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0471100|<br /> Description = Sterol desaturase family protein|<br /> Version = NM_001071361.1 GI:115482465 GeneID:4348869|<br /> Length = 5861 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0471100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:17906207..17912067|<br /> CDS = 17906494..17906655,17906746..17906922,17907019..17907117,17907202..17907402,17907731..17907838&lt;br&gt;,17907926..17908307,17908870..17909089,17910772..17911004,17911137..17911360&lt;br&gt;,17911825..17911884|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactgggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccattatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATNPGLFTEWPWKKLGSFKYVLLAPWVAHGWYEVATKGWREVD LGYIAILPSLLLRMLHNQAWITISRLQNARGRRQIVRRGIEFDQVDRERNWDDQIILS GILLYLGALYVPGGQHLPLWRTDGAGLIALLHAGPVEFLYYWFHRALHHHFLYTHYHS HHHSSIVTEPITSVIHPFAELVAYELLFSIPLIACALTGTASIIAFEMYLIYIDFMNN MGHCNFELVPSWLFTWFPPLKYLMYTPSFHSLHHTQFRTNYSLFMPFYDYIYNTMDKS SDTLYENSLKNNEEEEAVDVVHLTHLTTLHSIYHMRPGFAEFASRPYVSRWYMRMMWP LSWLSMVLTWTYGSSFTVERNVMKKIRMQSWAIPRYSFHYGLDWEKEAINDLIEKAVC EADKNGAKVVSLGLLNQAHTLNKSGEQYLLKYPKLGARIVDGTSLAAAVVVNSIPQGT DQVILAGNVSKVARAVAQALCKKNIKVTMTNKQDYHLLKPEIPETVADNLSFSKTGTA KVWLIGDGLDSAEQFRAQKGTLFIPYSQFPPKMVRKDSCSYSTTPAMAVPKTLQNVHS CENWLPRRVMSAWRIAGILHALEGWNEHECGDKVLDMDKVWSAAIMHGFCPVAQG&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5413..5574#5146..5322#4951..5049#4666..4866#4230..4337#3761..4142#2979..3198#1064..1296#708..931#184..243#atatgagactctgacacgtacgcacagctagatcgaagccctggtcgagtttataaattgagatatatccgtcctccattctcaggccagttcttgcaagactcttgccgtgttgagactgcagaaacgagagctagctagccagaggctgcaagaagattctgcacccatagatcgtctaccatggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaaggtaatcaaaattaaccttccatcttcttctccttaattcagttacttcactgtcttctggcagctcgatcgagcaaataaacgcacagcttctagttccatccatggcagcactgcaccatgaccattatgttcttgtttgagttcgctagcttctgtttgatatcaaagcatacattgccgcatgtgcagctagcagtgcatgagattaattctcttgcatctgctgcagatcagtaatgcaggggcatgaactttcctgcagcacctctgtgcagcaaataaactgttaaagagtacatggttaaataagcttttgccacatcgtctttgattaggcaaaaacattcagtatacacaacttccgacctttacaaattacaacagtaacttcagctataaattatagcacatgtgagttaattcgatggatggattcatgcatgtatatgtgtgcatatgcagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactggtatgccaaacaccataaaattacgtacaacccacgcggtgtgttcgtggcggctagcttcaccttcgagattaattaattcaacctcattatagttgatgtgttgttacgtgtatggtggtgtgcgtgcagggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatgtacgtaattaattgccactagagacggtttatcctcgaggctccaaacattacattctcttttaggcaataacctttgagcttctatattagtatcgaattgtgttgcgtcataaatatttttacaaaatttttattcatcatctaaagttatcaatgatcaaagttcaatcaatactaatagttacaagtaatttgaaatagaggaagtaagttgtactacctagttgtaccatcattgctccaaacaacctattacatgatattactttcgtcccaaacctctaaatgaatattaagattttggtaatacaaattcttaaaattttgagacagaaatattactagacctgtaaccttgggataaattaaaccacggtaatcttccaatagaacaaagtaaaaaacaagtcttttccataaggacacaaaacaggaggtgtacttcgtcagatgcaagtgtcgcgcgtgtccgttcctcaggaaacacagagtgttcctgtggccagtcgatcgctacttcaattccctcctccttcaatgcatgcctgaacaataattgtaggcctagagagtgtgtgagttaaggaatgcaaaggacacacgatatttcatggaccattgagatcgaagtaagccgaccgatcgatcgcgagctaaatttatgtagcccatcgaatccgttgctgaatgcctgaccaaacggagaagcaaacgtggtccatgaacaacgtatcagagacttttagacaagagagaaatcacattctaaggggttttttcccccttttttttgagtggaagagttttttttattgagaggtatcgagaagtactatatttttcgtgtaagctagccaaatatcagtgcgttgcaacttgcaacggattattaaaaatagcaaaattaagtccttgttaaattttttttaaaaaaaaatggttgaaacacaacttttcatcttaaagaaacaaacaaaaatgccatgcttagaaaattaaagagctaaagtatagtacagtatatatagtacaggcgactctcccaaaccttaataccatggcccatatatcagtgacccaggccctgggcaaactggtccacggcccagggcctgagatgaaaggcccgatgagaaatatgcatttttactaggaataagtctatttttcctcctttcaaccatgtaactttgtgttgggccaaaatttccatggggcggcctaggccttggcctatttctgggtccgccccttatcagtgattgtggtgatggcagattcactaccaccactactatcttttaatagtagtaaagattttggtgtcatcttgtaattttaagatgtacaagacggtaccataaattgactttaaagtgatttgaatggatgtgtaatgggagaaatcgcttttcaaatgtaaaataatgcactacttgtattagtactaaattttacattagagaacacgatatattcagtgattcttgataaccataaaatccctttaagattatggtgttttctttaagtactgttaaaaaaaaaccaatagtgtctactggttacactgcagaagaagagaaccctgcagactgcagtatttgtaattgagcaagcagatacatatacctaaatgcatatatgttcattacctacaattcctaacgttgttgcagccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtaagtacttcatcaggctcttaataatacttccttcttccttaaatgtttgacgccgttgacttttttaaacatgtttgaccgttcgtcttattcaaaaacttttgtgaaatgtgtaaaactatatgtatacataaaagtatatttaacaataaatcaaatgatagaaaaagaattaataattatttaaatttttgaataagacgaacggtcaaatatttttaaaaaagtcaatggcatcaaatattttgggatggagggagtatgtaaaagagtaactttacagtttttaagaaagcataggggaagtaccatattttatattggagaaattttacggttttcagtatttcttttaagataaaggaggctttattgatcttggatacaatatactctaaaaccactcctgatatctgcatagctaggatgcacatggtctatagttattgaaaaagccaaataaaagaagatgatgtgcatgtcactctgcaaaataaataacttgatcatacatgctgttttcactgttataataatctgaacttatgtgatatattaggtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccatgtaagagtaaccatgttcagtgcatgattcacagatggtgtgcatgataaattgataatcaggttagaaacattttgttttgtgcagtatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggtagctcatttcctaatgaattcagagcaagttgaaacattatcatccatacttcagaattcagatgttagctatatgtccacatgtactgccaaattttagtcaagataaaacagttagtgcttaataattgtttatcttcagggaaaaggtttagtcataaaggtaggaaaataaaaaaattaagtagcaaagagtccgttgacagtagtccaacagaactaataaattctgtacatttcatgagaatacaatatgtagaaattagggaagtatgtactcatgcatggttgaactggtaattgacatacaatatcttcaatataaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtaattaatccactactcacctctgatttattttatagcatgcaaaaggataccatgttgtaacaaaccactattcttgtacaggtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtaagaatagatttcctcatttttcctacattctctggacaaatgaatagaatgtataagagaaatatcattgcctaataaaccaaaaattttcaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgaggtagtagtaataacctgttgaaataatccagagattcagacgaacttcacatgatctaatcacaaaatttttgaactttttttttgacagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttgatgattcagttgcaaagtatcagaataagttttagctggattatgctatgtggtcaccgtatcagaaagagagatgttctgttggcaagaagagatatagtatctgagtaatttcatctgccatggtttttaaaacattgttcatcatctcctttgatcaaagactggccatggctactttctgtataagtatctgtcctttttagtccatagtgtaattcagcaacaaaacactcttggcattgttatgcagtctcatgaatgtttttccagcatacggaattacgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071361.1 RefSeq:Os10g0471100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184064 2014-06-14T05:26:39Z

<p>DYM: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced.<br /> <br /> ===Expression===<br /> Wda1 is strongly expressed in the epidermal cells of rice anthers. Wda1 transcript is abundant during flowering stages.Because the GUS gene located in T-DNA was fused to Wda1, the level and pattern of expression of the fusion gene could be assayed for GUS activity. Experiments have showed temporal and spatial expression patterns of the Wda1-GUS fusion product in heterozygous spikelets at various flowering stages(Fig.1). High levels of GUS activity were observed in spikelets from the premeiosis stage to the heading stage (Fig. 1A and 1K ). GUS activity was also prominent in the outer and inner epidermis (Fig. 1H) and trichomes (Fig. 1G) of the palea/lemma, lodicules (Fig. 1C, 1E, 1F, and 1I), stigmas (Fig. 1C), and anthers (Fig. 1C, 1D, 1I, and 1J). Cross sections of heterozygotic anthers at the vacuolated pollen stage revealed that GUS was expressed strongly in the epidermis but only weakly in the tapetum, endothecium, and connective tissue (Fig. 1J). In vegetative tissue, GUS activity was mostly absent except at collar regions (between the leaf sheath and the leaf blade) and the base of shoots. Semiquantitative RT-PCR analyses have been performed to examine whether GUS activity truly represented Wda1 expression patterns. Wda1 was highly expressed in panicles at various developmental stages (Fig. 1K) but only very weakly in the roots and shoots of 7-d-old seedlings. Only low transcript levels were detected in the developing seeds and flag leaves. In developing spikelets, transcript was detected in all floral organs (Fig. 1K and 1L), but it was barely detectable in the wda1 anthers (Fig. 1L). LOC_Os02g40780, which is the most similar rice gene to Wda1, was also expressed throughout panicle development. However, its expression was much higher in the shoots and flag leaves. In mature spikelets, transcript levels were low in the anthers and palea/lemma and barely detectable in the ovaries and lodicules (Fig. 1L). These results indicate that Wda1 functions primarily in the panicles, especially in the reproductive organs, whereas LOC_Os02g40780 may play roles in both vegetative and reproductive organs.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0471100|<br /> Description = Sterol desaturase family protein|<br /> Version = NM_001071361.1 GI:115482465 GeneID:4348869|<br /> Length = 5861 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0471100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:17906207..17912067|<br /> CDS = 17906494..17906655,17906746..17906922,17907019..17907117,17907202..17907402,17907731..17907838&lt;br&gt;,17907926..17908307,17908870..17909089,17910772..17911004,17911137..17911360&lt;br&gt;,17911825..17911884|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactgggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccattatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATNPGLFTEWPWKKLGSFKYVLLAPWVAHGWYEVATKGWREVD LGYIAILPSLLLRMLHNQAWITISRLQNARGRRQIVRRGIEFDQVDRERNWDDQIILS GILLYLGALYVPGGQHLPLWRTDGAGLIALLHAGPVEFLYYWFHRALHHHFLYTHYHS HHHSSIVTEPITSVIHPFAELVAYELLFSIPLIACALTGTASIIAFEMYLIYIDFMNN MGHCNFELVPSWLFTWFPPLKYLMYTPSFHSLHHTQFRTNYSLFMPFYDYIYNTMDKS SDTLYENSLKNNEEEEAVDVVHLTHLTTLHSIYHMRPGFAEFASRPYVSRWYMRMMWP LSWLSMVLTWTYGSSFTVERNVMKKIRMQSWAIPRYSFHYGLDWEKEAINDLIEKAVC EADKNGAKVVSLGLLNQAHTLNKSGEQYLLKYPKLGARIVDGTSLAAAVVVNSIPQGT DQVILAGNVSKVARAVAQALCKKNIKVTMTNKQDYHLLKPEIPETVADNLSFSKTGTA KVWLIGDGLDSAEQFRAQKGTLFIPYSQFPPKMVRKDSCSYSTTPAMAVPKTLQNVHS CENWLPRRVMSAWRIAGILHALEGWNEHECGDKVLDMDKVWSAAIMHGFCPVAQG&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5413..5574#5146..5322#4951..5049#4666..4866#4230..4337#3761..4142#2979..3198#1064..1296#708..931#184..243#atatgagactctgacacgtacgcacagctagatcgaagccctggtcgagtttataaattgagatatatccgtcctccattctcaggccagttcttgcaagactcttgccgtgttgagactgcagaaacgagagctagctagccagaggctgcaagaagattctgcacccatagatcgtctaccatggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaaggtaatcaaaattaaccttccatcttcttctccttaattcagttacttcactgtcttctggcagctcgatcgagcaaataaacgcacagcttctagttccatccatggcagcactgcaccatgaccattatgttcttgtttgagttcgctagcttctgtttgatatcaaagcatacattgccgcatgtgcagctagcagtgcatgagattaattctcttgcatctgctgcagatcagtaatgcaggggcatgaactttcctgcagcacctctgtgcagcaaataaactgttaaagagtacatggttaaataagcttttgccacatcgtctttgattaggcaaaaacattcagtatacacaacttccgacctttacaaattacaacagtaacttcagctataaattatagcacatgtgagttaattcgatggatggattcatgcatgtatatgtgtgcatatgcagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactggtatgccaaacaccataaaattacgtacaacccacgcggtgtgttcgtggcggctagcttcaccttcgagattaattaattcaacctcattatagttgatgtgttgttacgtgtatggtggtgtgcgtgcagggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatgtacgtaattaattgccactagagacggtttatcctcgaggctccaaacattacattctcttttaggcaataacctttgagcttctatattagtatcgaattgtgttgcgtcataaatatttttacaaaatttttattcatcatctaaagttatcaatgatcaaagttcaatcaatactaatagttacaagtaatttgaaatagaggaagtaagttgtactacctagttgtaccatcattgctccaaacaacctattacatgatattactttcgtcccaaacctctaaatgaatattaagattttggtaatacaaattcttaaaattttgagacagaaatattactagacctgtaaccttgggataaattaaaccacggtaatcttccaatagaacaaagtaaaaaacaagtcttttccataaggacacaaaacaggaggtgtacttcgtcagatgcaagtgtcgcgcgtgtccgttcctcaggaaacacagagtgttcctgtggccagtcgatcgctacttcaattccctcctccttcaatgcatgcctgaacaataattgtaggcctagagagtgtgtgagttaaggaatgcaaaggacacacgatatttcatggaccattgagatcgaagtaagccgaccgatcgatcgcgagctaaatttatgtagcccatcgaatccgttgctgaatgcctgaccaaacggagaagcaaacgtggtccatgaacaacgtatcagagacttttagacaagagagaaatcacattctaaggggttttttcccccttttttttgagtggaagagttttttttattgagaggtatcgagaagtactatatttttcgtgtaagctagccaaatatcagtgcgttgcaacttgcaacggattattaaaaatagcaaaattaagtccttgttaaattttttttaaaaaaaaatggttgaaacacaacttttcatcttaaagaaacaaacaaaaatgccatgcttagaaaattaaagagctaaagtatagtacagtatatatagtacaggcgactctcccaaaccttaataccatggcccatatatcagtgacccaggccctgggcaaactggtccacggcccagggcctgagatgaaaggcccgatgagaaatatgcatttttactaggaataagtctatttttcctcctttcaaccatgtaactttgtgttgggccaaaatttccatggggcggcctaggccttggcctatttctgggtccgccccttatcagtgattgtggtgatggcagattcactaccaccactactatcttttaatagtagtaaagattttggtgtcatcttgtaattttaagatgtacaagacggtaccataaattgactttaaagtgatttgaatggatgtgtaatgggagaaatcgcttttcaaatgtaaaataatgcactacttgtattagtactaaattttacattagagaacacgatatattcagtgattcttgataaccataaaatccctttaagattatggtgttttctttaagtactgttaaaaaaaaaccaatagtgtctactggttacactgcagaagaagagaaccctgcagactgcagtatttgtaattgagcaagcagatacatatacctaaatgcatatatgttcattacctacaattcctaacgttgttgcagccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtaagtacttcatcaggctcttaataatacttccttcttccttaaatgtttgacgccgttgacttttttaaacatgtttgaccgttcgtcttattcaaaaacttttgtgaaatgtgtaaaactatatgtatacataaaagtatatttaacaataaatcaaatgatagaaaaagaattaataattatttaaatttttgaataagacgaacggtcaaatatttttaaaaaagtcaatggcatcaaatattttgggatggagggagtatgtaaaagagtaactttacagtttttaagaaagcataggggaagtaccatattttatattggagaaattttacggttttcagtatttcttttaagataaaggaggctttattgatcttggatacaatatactctaaaaccactcctgatatctgcatagctaggatgcacatggtctatagttattgaaaaagccaaataaaagaagatgatgtgcatgtcactctgcaaaataaataacttgatcatacatgctgttttcactgttataataatctgaacttatgtgatatattaggtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccatgtaagagtaaccatgttcagtgcatgattcacagatggtgtgcatgataaattgataatcaggttagaaacattttgttttgtgcagtatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggtagctcatttcctaatgaattcagagcaagttgaaacattatcatccatacttcagaattcagatgttagctatatgtccacatgtactgccaaattttagtcaagataaaacagttagtgcttaataattgtttatcttcagggaaaaggtttagtcataaaggtaggaaaataaaaaaattaagtagcaaagagtccgttgacagtagtccaacagaactaataaattctgtacatttcatgagaatacaatatgtagaaattagggaagtatgtactcatgcatggttgaactggtaattgacatacaatatcttcaatataaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtaattaatccactactcacctctgatttattttatagcatgcaaaaggataccatgttgtaacaaaccactattcttgtacaggtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtaagaatagatttcctcatttttcctacattctctggacaaatgaatagaatgtataagagaaatatcattgcctaataaaccaaaaattttcaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgaggtagtagtaataacctgttgaaataatccagagattcagacgaacttcacatgatctaatcacaaaatttttgaactttttttttgacagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttgatgattcagttgcaaagtatcagaataagttttagctggattatgctatgtggtcaccgtatcagaaagagagatgttctgttggcaagaagagatatagtatctgagtaatttcatctgccatggtttttaaaacattgttcatcatctcctttgatcaaagactggccatggctactttctgtataagtatctgtcctttttagtccatagtgtaattcagcaacaaaacactcttggcattgttatgcagtctcatgaatgtttttccagcatacggaattacgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071361.1 RefSeq:Os10g0471100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184063 2014-06-14T04:59:25Z

<p>DYM: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced.<br /> <br /> ===Expression===<br /> Wda1 is a male-sterile mutant of rice, wax-deficient anther1 (wda1), that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 encodes a protein with high similarity to proteins involved in wax production. Wda1 transcript is abundant during flowering stages.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0471100|<br /> Description = Sterol desaturase family protein|<br /> Version = NM_001071361.1 GI:115482465 GeneID:4348869|<br /> Length = 5861 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0471100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:17906207..17912067|<br /> CDS = 17906494..17906655,17906746..17906922,17907019..17907117,17907202..17907402,17907731..17907838&lt;br&gt;,17907926..17908307,17908870..17909089,17910772..17911004,17911137..17911360&lt;br&gt;,17911825..17911884|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactgggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccattatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATNPGLFTEWPWKKLGSFKYVLLAPWVAHGWYEVATKGWREVD LGYIAILPSLLLRMLHNQAWITISRLQNARGRRQIVRRGIEFDQVDRERNWDDQIILS GILLYLGALYVPGGQHLPLWRTDGAGLIALLHAGPVEFLYYWFHRALHHHFLYTHYHS HHHSSIVTEPITSVIHPFAELVAYELLFSIPLIACALTGTASIIAFEMYLIYIDFMNN MGHCNFELVPSWLFTWFPPLKYLMYTPSFHSLHHTQFRTNYSLFMPFYDYIYNTMDKS SDTLYENSLKNNEEEEAVDVVHLTHLTTLHSIYHMRPGFAEFASRPYVSRWYMRMMWP LSWLSMVLTWTYGSSFTVERNVMKKIRMQSWAIPRYSFHYGLDWEKEAINDLIEKAVC EADKNGAKVVSLGLLNQAHTLNKSGEQYLLKYPKLGARIVDGTSLAAAVVVNSIPQGT DQVILAGNVSKVARAVAQALCKKNIKVTMTNKQDYHLLKPEIPETVADNLSFSKTGTA KVWLIGDGLDSAEQFRAQKGTLFIPYSQFPPKMVRKDSCSYSTTPAMAVPKTLQNVHS CENWLPRRVMSAWRIAGILHALEGWNEHECGDKVLDMDKVWSAAIMHGFCPVAQG&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5413..5574#5146..5322#4951..5049#4666..4866#4230..4337#3761..4142#2979..3198#1064..1296#708..931#184..243#atatgagactctgacacgtacgcacagctagatcgaagccctggtcgagtttataaattgagatatatccgtcctccattctcaggccagttcttgcaagactcttgccgtgttgagactgcagaaacgagagctagctagccagaggctgcaagaagattctgcacccatagatcgtctaccatggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaaggtaatcaaaattaaccttccatcttcttctccttaattcagttacttcactgtcttctggcagctcgatcgagcaaataaacgcacagcttctagttccatccatggcagcactgcaccatgaccattatgttcttgtttgagttcgctagcttctgtttgatatcaaagcatacattgccgcatgtgcagctagcagtgcatgagattaattctcttgcatctgctgcagatcagtaatgcaggggcatgaactttcctgcagcacctctgtgcagcaaataaactgttaaagagtacatggttaaataagcttttgccacatcgtctttgattaggcaaaaacattcagtatacacaacttccgacctttacaaattacaacagtaacttcagctataaattatagcacatgtgagttaattcgatggatggattcatgcatgtatatgtgtgcatatgcagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactggtatgccaaacaccataaaattacgtacaacccacgcggtgtgttcgtggcggctagcttcaccttcgagattaattaattcaacctcattatagttgatgtgttgttacgtgtatggtggtgtgcgtgcagggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatgtacgtaattaattgccactagagacggtttatcctcgaggctccaaacattacattctcttttaggcaataacctttgagcttctatattagtatcgaattgtgttgcgtcataaatatttttacaaaatttttattcatcatctaaagttatcaatgatcaaagttcaatcaatactaatagttacaagtaatttgaaatagaggaagtaagttgtactacctagttgtaccatcattgctccaaacaacctattacatgatattactttcgtcccaaacctctaaatgaatattaagattttggtaatacaaattcttaaaattttgagacagaaatattactagacctgtaaccttgggataaattaaaccacggtaatcttccaatagaacaaagtaaaaaacaagtcttttccataaggacacaaaacaggaggtgtacttcgtcagatgcaagtgtcgcgcgtgtccgttcctcaggaaacacagagtgttcctgtggccagtcgatcgctacttcaattccctcctccttcaatgcatgcctgaacaataattgtaggcctagagagtgtgtgagttaaggaatgcaaaggacacacgatatttcatggaccattgagatcgaagtaagccgaccgatcgatcgcgagctaaatttatgtagcccatcgaatccgttgctgaatgcctgaccaaacggagaagcaaacgtggtccatgaacaacgtatcagagacttttagacaagagagaaatcacattctaaggggttttttcccccttttttttgagtggaagagttttttttattgagaggtatcgagaagtactatatttttcgtgtaagctagccaaatatcagtgcgttgcaacttgcaacggattattaaaaatagcaaaattaagtccttgttaaattttttttaaaaaaaaatggttgaaacacaacttttcatcttaaagaaacaaacaaaaatgccatgcttagaaaattaaagagctaaagtatagtacagtatatatagtacaggcgactctcccaaaccttaataccatggcccatatatcagtgacccaggccctgggcaaactggtccacggcccagggcctgagatgaaaggcccgatgagaaatatgcatttttactaggaataagtctatttttcctcctttcaaccatgtaactttgtgttgggccaaaatttccatggggcggcctaggccttggcctatttctgggtccgccccttatcagtgattgtggtgatggcagattcactaccaccactactatcttttaatagtagtaaagattttggtgtcatcttgtaattttaagatgtacaagacggtaccataaattgactttaaagtgatttgaatggatgtgtaatgggagaaatcgcttttcaaatgtaaaataatgcactacttgtattagtactaaattttacattagagaacacgatatattcagtgattcttgataaccataaaatccctttaagattatggtgttttctttaagtactgttaaaaaaaaaccaatagtgtctactggttacactgcagaagaagagaaccctgcagactgcagtatttgtaattgagcaagcagatacatatacctaaatgcatatatgttcattacctacaattcctaacgttgttgcagccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtaagtacttcatcaggctcttaataatacttccttcttccttaaatgtttgacgccgttgacttttttaaacatgtttgaccgttcgtcttattcaaaaacttttgtgaaatgtgtaaaactatatgtatacataaaagtatatttaacaataaatcaaatgatagaaaaagaattaataattatttaaatttttgaataagacgaacggtcaaatatttttaaaaaagtcaatggcatcaaatattttgggatggagggagtatgtaaaagagtaactttacagtttttaagaaagcataggggaagtaccatattttatattggagaaattttacggttttcagtatttcttttaagataaaggaggctttattgatcttggatacaatatactctaaaaccactcctgatatctgcatagctaggatgcacatggtctatagttattgaaaaagccaaataaaagaagatgatgtgcatgtcactctgcaaaataaataacttgatcatacatgctgttttcactgttataataatctgaacttatgtgatatattaggtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccatgtaagagtaaccatgttcagtgcatgattcacagatggtgtgcatgataaattgataatcaggttagaaacattttgttttgtgcagtatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggtagctcatttcctaatgaattcagagcaagttgaaacattatcatccatacttcagaattcagatgttagctatatgtccacatgtactgccaaattttagtcaagataaaacagttagtgcttaataattgtttatcttcagggaaaaggtttagtcataaaggtaggaaaataaaaaaattaagtagcaaagagtccgttgacagtagtccaacagaactaataaattctgtacatttcatgagaatacaatatgtagaaattagggaagtatgtactcatgcatggttgaactggtaattgacatacaatatcttcaatataaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtaattaatccactactcacctctgatttattttatagcatgcaaaaggataccatgttgtaacaaaccactattcttgtacaggtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtaagaatagatttcctcatttttcctacattctctggacaaatgaatagaatgtataagagaaatatcattgcctaataaaccaaaaattttcaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgaggtagtagtaataacctgttgaaataatccagagattcagacgaacttcacatgatctaatcacaaaatttttgaactttttttttgacagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttgatgattcagttgcaaagtatcagaataagttttagctggattatgctatgtggtcaccgtatcagaaagagagatgttctgttggcaagaagagatatagtatctgagtaatttcatctgccatggtttttaaaacattgttcatcatctcctttgatcaaagactggccatggctactttctgtataagtatctgtcctttttagtccatagtgtaattcagcaacaaaacactcttggcattgttatgcagtctcatgaatgtttttccagcatacggaattacgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071361.1 RefSeq:Os10g0471100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184062 2014-06-14T04:58:18Z

<p>DYM: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is a male-sterile mutant of rice, that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition.<br /> <br /> ===Expression===<br /> Wda1 is a male-sterile mutant of rice, wax-deficient anther1 (wda1), that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 encodes a protein with high similarity to proteins involved in wax production. Wda1 transcript is abundant during flowering stages.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0471100|<br /> Description = Sterol desaturase family protein|<br /> Version = NM_001071361.1 GI:115482465 GeneID:4348869|<br /> Length = 5861 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0471100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:17906207..17912067|<br /> CDS = 17906494..17906655,17906746..17906922,17907019..17907117,17907202..17907402,17907731..17907838&lt;br&gt;,17907926..17908307,17908870..17909089,17910772..17911004,17911137..17911360&lt;br&gt;,17911825..17911884|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactgggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccattatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATNPGLFTEWPWKKLGSFKYVLLAPWVAHGWYEVATKGWREVD LGYIAILPSLLLRMLHNQAWITISRLQNARGRRQIVRRGIEFDQVDRERNWDDQIILS GILLYLGALYVPGGQHLPLWRTDGAGLIALLHAGPVEFLYYWFHRALHHHFLYTHYHS HHHSSIVTEPITSVIHPFAELVAYELLFSIPLIACALTGTASIIAFEMYLIYIDFMNN MGHCNFELVPSWLFTWFPPLKYLMYTPSFHSLHHTQFRTNYSLFMPFYDYIYNTMDKS SDTLYENSLKNNEEEEAVDVVHLTHLTTLHSIYHMRPGFAEFASRPYVSRWYMRMMWP LSWLSMVLTWTYGSSFTVERNVMKKIRMQSWAIPRYSFHYGLDWEKEAINDLIEKAVC EADKNGAKVVSLGLLNQAHTLNKSGEQYLLKYPKLGARIVDGTSLAAAVVVNSIPQGT DQVILAGNVSKVARAVAQALCKKNIKVTMTNKQDYHLLKPEIPETVADNLSFSKTGTA KVWLIGDGLDSAEQFRAQKGTLFIPYSQFPPKMVRKDSCSYSTTPAMAVPKTLQNVHS CENWLPRRVMSAWRIAGILHALEGWNEHECGDKVLDMDKVWSAAIMHGFCPVAQG&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5413..5574#5146..5322#4951..5049#4666..4866#4230..4337#3761..4142#2979..3198#1064..1296#708..931#184..243#atatgagactctgacacgtacgcacagctagatcgaagccctggtcgagtttataaattgagatatatccgtcctccattctcaggccagttcttgcaagactcttgccgtgttgagactgcagaaacgagagctagctagccagaggctgcaagaagattctgcacccatagatcgtctaccatggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaaggtaatcaaaattaaccttccatcttcttctccttaattcagttacttcactgtcttctggcagctcgatcgagcaaataaacgcacagcttctagttccatccatggcagcactgcaccatgaccattatgttcttgtttgagttcgctagcttctgtttgatatcaaagcatacattgccgcatgtgcagctagcagtgcatgagattaattctcttgcatctgctgcagatcagtaatgcaggggcatgaactttcctgcagcacctctgtgcagcaaataaactgttaaagagtacatggttaaataagcttttgccacatcgtctttgattaggcaaaaacattcagtatacacaacttccgacctttacaaattacaacagtaacttcagctataaattatagcacatgtgagttaattcgatggatggattcatgcatgtatatgtgtgcatatgcagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactggtatgccaaacaccataaaattacgtacaacccacgcggtgtgttcgtggcggctagcttcaccttcgagattaattaattcaacctcattatagttgatgtgttgttacgtgtatggtggtgtgcgtgcagggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatgtacgtaattaattgccactagagacggtttatcctcgaggctccaaacattacattctcttttaggcaataacctttgagcttctatattagtatcgaattgtgttgcgtcataaatatttttacaaaatttttattcatcatctaaagttatcaatgatcaaagttcaatcaatactaatagttacaagtaatttgaaatagaggaagtaagttgtactacctagttgtaccatcattgctccaaacaacctattacatgatattactttcgtcccaaacctctaaatgaatattaagattttggtaatacaaattcttaaaattttgagacagaaatattactagacctgtaaccttgggataaattaaaccacggtaatcttccaatagaacaaagtaaaaaacaagtcttttccataaggacacaaaacaggaggtgtacttcgtcagatgcaagtgtcgcgcgtgtccgttcctcaggaaacacagagtgttcctgtggccagtcgatcgctacttcaattccctcctccttcaatgcatgcctgaacaataattgtaggcctagagagtgtgtgagttaaggaatgcaaaggacacacgatatttcatggaccattgagatcgaagtaagccgaccgatcgatcgcgagctaaatttatgtagcccatcgaatccgttgctgaatgcctgaccaaacggagaagcaaacgtggtccatgaacaacgtatcagagacttttagacaagagagaaatcacattctaaggggttttttcccccttttttttgagtggaagagttttttttattgagaggtatcgagaagtactatatttttcgtgtaagctagccaaatatcagtgcgttgcaacttgcaacggattattaaaaatagcaaaattaagtccttgttaaattttttttaaaaaaaaatggttgaaacacaacttttcatcttaaagaaacaaacaaaaatgccatgcttagaaaattaaagagctaaagtatagtacagtatatatagtacaggcgactctcccaaaccttaataccatggcccatatatcagtgacccaggccctgggcaaactggtccacggcccagggcctgagatgaaaggcccgatgagaaatatgcatttttactaggaataagtctatttttcctcctttcaaccatgtaactttgtgttgggccaaaatttccatggggcggcctaggccttggcctatttctgggtccgccccttatcagtgattgtggtgatggcagattcactaccaccactactatcttttaatagtagtaaagattttggtgtcatcttgtaattttaagatgtacaagacggtaccataaattgactttaaagtgatttgaatggatgtgtaatgggagaaatcgcttttcaaatgtaaaataatgcactacttgtattagtactaaattttacattagagaacacgatatattcagtgattcttgataaccataaaatccctttaagattatggtgttttctttaagtactgttaaaaaaaaaccaatagtgtctactggttacactgcagaagaagagaaccctgcagactgcagtatttgtaattgagcaagcagatacatatacctaaatgcatatatgttcattacctacaattcctaacgttgttgcagccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtaagtacttcatcaggctcttaataatacttccttcttccttaaatgtttgacgccgttgacttttttaaacatgtttgaccgttcgtcttattcaaaaacttttgtgaaatgtgtaaaactatatgtatacataaaagtatatttaacaataaatcaaatgatagaaaaagaattaataattatttaaatttttgaataagacgaacggtcaaatatttttaaaaaagtcaatggcatcaaatattttgggatggagggagtatgtaaaagagtaactttacagtttttaagaaagcataggggaagtaccatattttatattggagaaattttacggttttcagtatttcttttaagataaaggaggctttattgatcttggatacaatatactctaaaaccactcctgatatctgcatagctaggatgcacatggtctatagttattgaaaaagccaaataaaagaagatgatgtgcatgtcactctgcaaaataaataacttgatcatacatgctgttttcactgttataataatctgaacttatgtgatatattaggtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccatgtaagagtaaccatgttcagtgcatgattcacagatggtgtgcatgataaattgataatcaggttagaaacattttgttttgtgcagtatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggtagctcatttcctaatgaattcagagcaagttgaaacattatcatccatacttcagaattcagatgttagctatatgtccacatgtactgccaaattttagtcaagataaaacagttagtgcttaataattgtttatcttcagggaaaaggtttagtcataaaggtaggaaaataaaaaaattaagtagcaaagagtccgttgacagtagtccaacagaactaataaattctgtacatttcatgagaatacaatatgtagaaattagggaagtatgtactcatgcatggttgaactggtaattgacatacaatatcttcaatataaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtaattaatccactactcacctctgatttattttatagcatgcaaaaggataccatgttgtaacaaaccactattcttgtacaggtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtaagaatagatttcctcatttttcctacattctctggacaaatgaatagaatgtataagagaaatatcattgcctaataaaccaaaaattttcaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgaggtagtagtaataacctgttgaaataatccagagattcagacgaacttcacatgatctaatcacaaaatttttgaactttttttttgacagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttgatgattcagttgcaaagtatcagaataagttttagctggattatgctatgtggtcaccgtatcagaaagagagatgttctgttggcaagaagagatatagtatctgagtaatttcatctgccatggtttttaaaacattgttcatcatctcctttgatcaaagactggccatggctactttctgtataagtatctgtcctttttagtccatagtgtaattcagcaacaaaacactcttggcattgttatgcagtctcatgaatgtttttccagcatacggaattacgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071361.1 RefSeq:Os10g0471100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184061 2014-06-14T04:49:01Z

<p>DYM: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is strongly expressed in the epidermal cells of rice anthers. The wda1 mutant exhibits developmental and biochemical phenotypes that reveal the functioning of Wda1 in the epidermal layer and tapetum of the anther wall, the orbicule in the peritapetal region and pollen development, and the biosynthesis of VLCFA derivatives required for cuticle formation and wax deposition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced.<br /> <br /> ===Expression===<br /> Wda1 is a male-sterile mutant of rice, wax-deficient anther1 (wda1), that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 encodes a protein with high similarity to proteins involved in wax production. Wda1 transcript is abundant during flowering stages.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0471100|<br /> Description = Sterol desaturase family protein|<br /> Version = NM_001071361.1 GI:115482465 GeneID:4348869|<br /> Length = 5861 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0471100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:17906207..17912067|<br /> CDS = 17906494..17906655,17906746..17906922,17907019..17907117,17907202..17907402,17907731..17907838&lt;br&gt;,17907926..17908307,17908870..17909089,17910772..17911004,17911137..17911360&lt;br&gt;,17911825..17911884|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactgggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccattatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATNPGLFTEWPWKKLGSFKYVLLAPWVAHGWYEVATKGWREVD LGYIAILPSLLLRMLHNQAWITISRLQNARGRRQIVRRGIEFDQVDRERNWDDQIILS GILLYLGALYVPGGQHLPLWRTDGAGLIALLHAGPVEFLYYWFHRALHHHFLYTHYHS HHHSSIVTEPITSVIHPFAELVAYELLFSIPLIACALTGTASIIAFEMYLIYIDFMNN MGHCNFELVPSWLFTWFPPLKYLMYTPSFHSLHHTQFRTNYSLFMPFYDYIYNTMDKS SDTLYENSLKNNEEEEAVDVVHLTHLTTLHSIYHMRPGFAEFASRPYVSRWYMRMMWP LSWLSMVLTWTYGSSFTVERNVMKKIRMQSWAIPRYSFHYGLDWEKEAINDLIEKAVC EADKNGAKVVSLGLLNQAHTLNKSGEQYLLKYPKLGARIVDGTSLAAAVVVNSIPQGT DQVILAGNVSKVARAVAQALCKKNIKVTMTNKQDYHLLKPEIPETVADNLSFSKTGTA KVWLIGDGLDSAEQFRAQKGTLFIPYSQFPPKMVRKDSCSYSTTPAMAVPKTLQNVHS CENWLPRRVMSAWRIAGILHALEGWNEHECGDKVLDMDKVWSAAIMHGFCPVAQG&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5413..5574#5146..5322#4951..5049#4666..4866#4230..4337#3761..4142#2979..3198#1064..1296#708..931#184..243#atatgagactctgacacgtacgcacagctagatcgaagccctggtcgagtttataaattgagatatatccgtcctccattctcaggccagttcttgcaagactcttgccgtgttgagactgcagaaacgagagctagctagccagaggctgcaagaagattctgcacccatagatcgtctaccatggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaaggtaatcaaaattaaccttccatcttcttctccttaattcagttacttcactgtcttctggcagctcgatcgagcaaataaacgcacagcttctagttccatccatggcagcactgcaccatgaccattatgttcttgtttgagttcgctagcttctgtttgatatcaaagcatacattgccgcatgtgcagctagcagtgcatgagattaattctcttgcatctgctgcagatcagtaatgcaggggcatgaactttcctgcagcacctctgtgcagcaaataaactgttaaagagtacatggttaaataagcttttgccacatcgtctttgattaggcaaaaacattcagtatacacaacttccgacctttacaaattacaacagtaacttcagctataaattatagcacatgtgagttaattcgatggatggattcatgcatgtatatgtgtgcatatgcagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactggtatgccaaacaccataaaattacgtacaacccacgcggtgtgttcgtggcggctagcttcaccttcgagattaattaattcaacctcattatagttgatgtgttgttacgtgtatggtggtgtgcgtgcagggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatgtacgtaattaattgccactagagacggtttatcctcgaggctccaaacattacattctcttttaggcaataacctttgagcttctatattagtatcgaattgtgttgcgtcataaatatttttacaaaatttttattcatcatctaaagttatcaatgatcaaagttcaatcaatactaatagttacaagtaatttgaaatagaggaagtaagttgtactacctagttgtaccatcattgctccaaacaacctattacatgatattactttcgtcccaaacctctaaatgaatattaagattttggtaatacaaattcttaaaattttgagacagaaatattactagacctgtaaccttgggataaattaaaccacggtaatcttccaatagaacaaagtaaaaaacaagtcttttccataaggacacaaaacaggaggtgtacttcgtcagatgcaagtgtcgcgcgtgtccgttcctcaggaaacacagagtgttcctgtggccagtcgatcgctacttcaattccctcctccttcaatgcatgcctgaacaataattgtaggcctagagagtgtgtgagttaaggaatgcaaaggacacacgatatttcatggaccattgagatcgaagtaagccgaccgatcgatcgcgagctaaatttatgtagcccatcgaatccgttgctgaatgcctgaccaaacggagaagcaaacgtggtccatgaacaacgtatcagagacttttagacaagagagaaatcacattctaaggggttttttcccccttttttttgagtggaagagttttttttattgagaggtatcgagaagtactatatttttcgtgtaagctagccaaatatcagtgcgttgcaacttgcaacggattattaaaaatagcaaaattaagtccttgttaaattttttttaaaaaaaaatggttgaaacacaacttttcatcttaaagaaacaaacaaaaatgccatgcttagaaaattaaagagctaaagtatagtacagtatatatagtacaggcgactctcccaaaccttaataccatggcccatatatcagtgacccaggccctgggcaaactggtccacggcccagggcctgagatgaaaggcccgatgagaaatatgcatttttactaggaataagtctatttttcctcctttcaaccatgtaactttgtgttgggccaaaatttccatggggcggcctaggccttggcctatttctgggtccgccccttatcagtgattgtggtgatggcagattcactaccaccactactatcttttaatagtagtaaagattttggtgtcatcttgtaattttaagatgtacaagacggtaccataaattgactttaaagtgatttgaatggatgtgtaatgggagaaatcgcttttcaaatgtaaaataatgcactacttgtattagtactaaattttacattagagaacacgatatattcagtgattcttgataaccataaaatccctttaagattatggtgttttctttaagtactgttaaaaaaaaaccaatagtgtctactggttacactgcagaagaagagaaccctgcagactgcagtatttgtaattgagcaagcagatacatatacctaaatgcatatatgttcattacctacaattcctaacgttgttgcagccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtaagtacttcatcaggctcttaataatacttccttcttccttaaatgtttgacgccgttgacttttttaaacatgtttgaccgttcgtcttattcaaaaacttttgtgaaatgtgtaaaactatatgtatacataaaagtatatttaacaataaatcaaatgatagaaaaagaattaataattatttaaatttttgaataagacgaacggtcaaatatttttaaaaaagtcaatggcatcaaatattttgggatggagggagtatgtaaaagagtaactttacagtttttaagaaagcataggggaagtaccatattttatattggagaaattttacggttttcagtatttcttttaagataaaggaggctttattgatcttggatacaatatactctaaaaccactcctgatatctgcatagctaggatgcacatggtctatagttattgaaaaagccaaataaaagaagatgatgtgcatgtcactctgcaaaataaataacttgatcatacatgctgttttcactgttataataatctgaacttatgtgatatattaggtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccatgtaagagtaaccatgttcagtgcatgattcacagatggtgtgcatgataaattgataatcaggttagaaacattttgttttgtgcagtatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggtagctcatttcctaatgaattcagagcaagttgaaacattatcatccatacttcagaattcagatgttagctatatgtccacatgtactgccaaattttagtcaagataaaacagttagtgcttaataattgtttatcttcagggaaaaggtttagtcataaaggtaggaaaataaaaaaattaagtagcaaagagtccgttgacagtagtccaacagaactaataaattctgtacatttcatgagaatacaatatgtagaaattagggaagtatgtactcatgcatggttgaactggtaattgacatacaatatcttcaatataaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtaattaatccactactcacctctgatttattttatagcatgcaaaaggataccatgttgtaacaaaccactattcttgtacaggtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtaagaatagatttcctcatttttcctacattctctggacaaatgaatagaatgtataagagaaatatcattgcctaataaaccaaaaattttcaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgaggtagtagtaataacctgttgaaataatccagagattcagacgaacttcacatgatctaatcacaaaatttttgaactttttttttgacagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttgatgattcagttgcaaagtatcagaataagttttagctggattatgctatgtggtcaccgtatcagaaagagagatgttctgttggcaagaagagatatagtatctgagtaatttcatctgccatggtttttaaaacattgttcatcatctcctttgatcaaagactggccatggctactttctgtataagtatctgtcctttttagtccatagtgtaattcagcaacaaaacactcttggcattgttatgcagtctcatgaatgtttttccagcatacggaattacgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071361.1 RefSeq:Os10g0471100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184060 2014-06-14T04:30:36Z

<p>DYM: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is strongly expressed in the epidermal cells of rice anthers. The wda1 mutant exhibits developmental and biochemical phenotypes that reveal the functioning of Wda1 in the epidermal layer and tapetum of the anther wall, the orbicule in the peritapetal region and pollen development, and the biosynthesis of VLCFA derivatives required for cuticle formation and wax deposition. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced.<br /> <br /> ===Expression===<br /> Wda1 is a male-sterile mutant of rice, wax-deficient anther1 (wda1), that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 encodes a protein with high similarity to proteins involved in wax production.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0471100|<br /> Description = Sterol desaturase family protein|<br /> Version = NM_001071361.1 GI:115482465 GeneID:4348869|<br /> Length = 5861 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0471100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:17906207..17912067|<br /> CDS = 17906494..17906655,17906746..17906922,17907019..17907117,17907202..17907402,17907731..17907838&lt;br&gt;,17907926..17908307,17908870..17909089,17910772..17911004,17911137..17911360&lt;br&gt;,17911825..17911884|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactgggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccattatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATNPGLFTEWPWKKLGSFKYVLLAPWVAHGWYEVATKGWREVD LGYIAILPSLLLRMLHNQAWITISRLQNARGRRQIVRRGIEFDQVDRERNWDDQIILS GILLYLGALYVPGGQHLPLWRTDGAGLIALLHAGPVEFLYYWFHRALHHHFLYTHYHS HHHSSIVTEPITSVIHPFAELVAYELLFSIPLIACALTGTASIIAFEMYLIYIDFMNN MGHCNFELVPSWLFTWFPPLKYLMYTPSFHSLHHTQFRTNYSLFMPFYDYIYNTMDKS SDTLYENSLKNNEEEEAVDVVHLTHLTTLHSIYHMRPGFAEFASRPYVSRWYMRMMWP LSWLSMVLTWTYGSSFTVERNVMKKIRMQSWAIPRYSFHYGLDWEKEAINDLIEKAVC EADKNGAKVVSLGLLNQAHTLNKSGEQYLLKYPKLGARIVDGTSLAAAVVVNSIPQGT DQVILAGNVSKVARAVAQALCKKNIKVTMTNKQDYHLLKPEIPETVADNLSFSKTGTA KVWLIGDGLDSAEQFRAQKGTLFIPYSQFPPKMVRKDSCSYSTTPAMAVPKTLQNVHS CENWLPRRVMSAWRIAGILHALEGWNEHECGDKVLDMDKVWSAAIMHGFCPVAQG&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5413..5574#5146..5322#4951..5049#4666..4866#4230..4337#3761..4142#2979..3198#1064..1296#708..931#184..243#atatgagactctgacacgtacgcacagctagatcgaagccctggtcgagtttataaattgagatatatccgtcctccattctcaggccagttcttgcaagactcttgccgtgttgagactgcagaaacgagagctagctagccagaggctgcaagaagattctgcacccatagatcgtctaccatggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaaggtaatcaaaattaaccttccatcttcttctccttaattcagttacttcactgtcttctggcagctcgatcgagcaaataaacgcacagcttctagttccatccatggcagcactgcaccatgaccattatgttcttgtttgagttcgctagcttctgtttgatatcaaagcatacattgccgcatgtgcagctagcagtgcatgagattaattctcttgcatctgctgcagatcagtaatgcaggggcatgaactttcctgcagcacctctgtgcagcaaataaactgttaaagagtacatggttaaataagcttttgccacatcgtctttgattaggcaaaaacattcagtatacacaacttccgacctttacaaattacaacagtaacttcagctataaattatagcacatgtgagttaattcgatggatggattcatgcatgtatatgtgtgcatatgcagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactggtatgccaaacaccataaaattacgtacaacccacgcggtgtgttcgtggcggctagcttcaccttcgagattaattaattcaacctcattatagttgatgtgttgttacgtgtatggtggtgtgcgtgcagggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatgtacgtaattaattgccactagagacggtttatcctcgaggctccaaacattacattctcttttaggcaataacctttgagcttctatattagtatcgaattgtgttgcgtcataaatatttttacaaaatttttattcatcatctaaagttatcaatgatcaaagttcaatcaatactaatagttacaagtaatttgaaatagaggaagtaagttgtactacctagttgtaccatcattgctccaaacaacctattacatgatattactttcgtcccaaacctctaaatgaatattaagattttggtaatacaaattcttaaaattttgagacagaaatattactagacctgtaaccttgggataaattaaaccacggtaatcttccaatagaacaaagtaaaaaacaagtcttttccataaggacacaaaacaggaggtgtacttcgtcagatgcaagtgtcgcgcgtgtccgttcctcaggaaacacagagtgttcctgtggccagtcgatcgctacttcaattccctcctccttcaatgcatgcctgaacaataattgtaggcctagagagtgtgtgagttaaggaatgcaaaggacacacgatatttcatggaccattgagatcgaagtaagccgaccgatcgatcgcgagctaaatttatgtagcccatcgaatccgttgctgaatgcctgaccaaacggagaagcaaacgtggtccatgaacaacgtatcagagacttttagacaagagagaaatcacattctaaggggttttttcccccttttttttgagtggaagagttttttttattgagaggtatcgagaagtactatatttttcgtgtaagctagccaaatatcagtgcgttgcaacttgcaacggattattaaaaatagcaaaattaagtccttgttaaattttttttaaaaaaaaatggttgaaacacaacttttcatcttaaagaaacaaacaaaaatgccatgcttagaaaattaaagagctaaagtatagtacagtatatatagtacaggcgactctcccaaaccttaataccatggcccatatatcagtgacccaggccctgggcaaactggtccacggcccagggcctgagatgaaaggcccgatgagaaatatgcatttttactaggaataagtctatttttcctcctttcaaccatgtaactttgtgttgggccaaaatttccatggggcggcctaggccttggcctatttctgggtccgccccttatcagtgattgtggtgatggcagattcactaccaccactactatcttttaatagtagtaaagattttggtgtcatcttgtaattttaagatgtacaagacggtaccataaattgactttaaagtgatttgaatggatgtgtaatgggagaaatcgcttttcaaatgtaaaataatgcactacttgtattagtactaaattttacattagagaacacgatatattcagtgattcttgataaccataaaatccctttaagattatggtgttttctttaagtactgttaaaaaaaaaccaatagtgtctactggttacactgcagaagaagagaaccctgcagactgcagtatttgtaattgagcaagcagatacatatacctaaatgcatatatgttcattacctacaattcctaacgttgttgcagccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtaagtacttcatcaggctcttaataatacttccttcttccttaaatgtttgacgccgttgacttttttaaacatgtttgaccgttcgtcttattcaaaaacttttgtgaaatgtgtaaaactatatgtatacataaaagtatatttaacaataaatcaaatgatagaaaaagaattaataattatttaaatttttgaataagacgaacggtcaaatatttttaaaaaagtcaatggcatcaaatattttgggatggagggagtatgtaaaagagtaactttacagtttttaagaaagcataggggaagtaccatattttatattggagaaattttacggttttcagtatttcttttaagataaaggaggctttattgatcttggatacaatatactctaaaaccactcctgatatctgcatagctaggatgcacatggtctatagttattgaaaaagccaaataaaagaagatgatgtgcatgtcactctgcaaaataaataacttgatcatacatgctgttttcactgttataataatctgaacttatgtgatatattaggtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccatgtaagagtaaccatgttcagtgcatgattcacagatggtgtgcatgataaattgataatcaggttagaaacattttgttttgtgcagtatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggtagctcatttcctaatgaattcagagcaagttgaaacattatcatccatacttcagaattcagatgttagctatatgtccacatgtactgccaaattttagtcaagataaaacagttagtgcttaataattgtttatcttcagggaaaaggtttagtcataaaggtaggaaaataaaaaaattaagtagcaaagagtccgttgacagtagtccaacagaactaataaattctgtacatttcatgagaatacaatatgtagaaattagggaagtatgtactcatgcatggttgaactggtaattgacatacaatatcttcaatataaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtaattaatccactactcacctctgatttattttatagcatgcaaaaggataccatgttgtaacaaaccactattcttgtacaggtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtaagaatagatttcctcatttttcctacattctctggacaaatgaatagaatgtataagagaaatatcattgcctaataaaccaaaaattttcaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgaggtagtagtaataacctgttgaaataatccagagattcagacgaacttcacatgatctaatcacaaaatttttgaactttttttttgacagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttgatgattcagttgcaaagtatcagaataagttttagctggattatgctatgtggtcaccgtatcagaaagagagatgttctgttggcaagaagagatatagtatctgagtaatttcatctgccatggtttttaaaacattgttcatcatctcctttgatcaaagactggccatggctactttctgtataagtatctgtcctttttagtccatagtgtaattcagcaacaaaacactcttggcattgttatgcagtctcatgaatgtttttccagcatacggaattacgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071361.1 RefSeq:Os10g0471100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184059 2014-06-14T04:28:32Z

<p>DYM: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is strongly expressed in the epidermal cells of rice anthers. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. The wda1 mutant exhibits developmental and<br /> biochemical phenotypes that reveal the functioning of Wda1 in the epidermal layer and tapetum of the anther wall, the orbicule in the<br /> peritapetal region and pollen development, and the biosynthesis of VLCFA derivatives required for cuticle formation and wax deposition. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced.<br /> <br /> ===Expression===<br /> Wda1 is a male-sterile mutant of rice, wax-deficient anther1 (wda1), that was generated through T-DNA insertional mutation, and is preferentially expressed in epidermis of various floral organs, including Anthers. This mutant shows morphological changes in its pollen and anther walls as well as alterations in its wax composition. Wda1 encodes a protein with high similarity to proteins involved in wax production.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0471100|<br /> Description = Sterol desaturase family protein|<br /> Version = NM_001071361.1 GI:115482465 GeneID:4348869|<br /> Length = 5861 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0471100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:17906207..17912067|<br /> CDS = 17906494..17906655,17906746..17906922,17907019..17907117,17907202..17907402,17907731..17907838&lt;br&gt;,17907926..17908307,17908870..17909089,17910772..17911004,17911137..17911360&lt;br&gt;,17911825..17911884|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactgggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccattatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATNPGLFTEWPWKKLGSFKYVLLAPWVAHGWYEVATKGWREVD LGYIAILPSLLLRMLHNQAWITISRLQNARGRRQIVRRGIEFDQVDRERNWDDQIILS GILLYLGALYVPGGQHLPLWRTDGAGLIALLHAGPVEFLYYWFHRALHHHFLYTHYHS HHHSSIVTEPITSVIHPFAELVAYELLFSIPLIACALTGTASIIAFEMYLIYIDFMNN MGHCNFELVPSWLFTWFPPLKYLMYTPSFHSLHHTQFRTNYSLFMPFYDYIYNTMDKS SDTLYENSLKNNEEEEAVDVVHLTHLTTLHSIYHMRPGFAEFASRPYVSRWYMRMMWP LSWLSMVLTWTYGSSFTVERNVMKKIRMQSWAIPRYSFHYGLDWEKEAINDLIEKAVC EADKNGAKVVSLGLLNQAHTLNKSGEQYLLKYPKLGARIVDGTSLAAAVVVNSIPQGT DQVILAGNVSKVARAVAQALCKKNIKVTMTNKQDYHLLKPEIPETVADNLSFSKTGTA KVWLIGDGLDSAEQFRAQKGTLFIPYSQFPPKMVRKDSCSYSTTPAMAVPKTLQNVHS CENWLPRRVMSAWRIAGILHALEGWNEHECGDKVLDMDKVWSAAIMHGFCPVAQG&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5413..5574#5146..5322#4951..5049#4666..4866#4230..4337#3761..4142#2979..3198#1064..1296#708..931#184..243#atatgagactctgacacgtacgcacagctagatcgaagccctggtcgagtttataaattgagatatatccgtcctccattctcaggccagttcttgcaagactcttgccgtgttgagactgcagaaacgagagctagctagccagaggctgcaagaagattctgcacccatagatcgtctaccatggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaaggtaatcaaaattaaccttccatcttcttctccttaattcagttacttcactgtcttctggcagctcgatcgagcaaataaacgcacagcttctagttccatccatggcagcactgcaccatgaccattatgttcttgtttgagttcgctagcttctgtttgatatcaaagcatacattgccgcatgtgcagctagcagtgcatgagattaattctcttgcatctgctgcagatcagtaatgcaggggcatgaactttcctgcagcacctctgtgcagcaaataaactgttaaagagtacatggttaaataagcttttgccacatcgtctttgattaggcaaaaacattcagtatacacaacttccgacctttacaaattacaacagtaacttcagctataaattatagcacatgtgagttaattcgatggatggattcatgcatgtatatgtgtgcatatgcagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactggtatgccaaacaccataaaattacgtacaacccacgcggtgtgttcgtggcggctagcttcaccttcgagattaattaattcaacctcattatagttgatgtgttgttacgtgtatggtggtgtgcgtgcagggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatgtacgtaattaattgccactagagacggtttatcctcgaggctccaaacattacattctcttttaggcaataacctttgagcttctatattagtatcgaattgtgttgcgtcataaatatttttacaaaatttttattcatcatctaaagttatcaatgatcaaagttcaatcaatactaatagttacaagtaatttgaaatagaggaagtaagttgtactacctagttgtaccatcattgctccaaacaacctattacatgatattactttcgtcccaaacctctaaatgaatattaagattttggtaatacaaattcttaaaattttgagacagaaatattactagacctgtaaccttgggataaattaaaccacggtaatcttccaatagaacaaagtaaaaaacaagtcttttccataaggacacaaaacaggaggtgtacttcgtcagatgcaagtgtcgcgcgtgtccgttcctcaggaaacacagagtgttcctgtggccagtcgatcgctacttcaattccctcctccttcaatgcatgcctgaacaataattgtaggcctagagagtgtgtgagttaaggaatgcaaaggacacacgatatttcatggaccattgagatcgaagtaagccgaccgatcgatcgcgagctaaatttatgtagcccatcgaatccgttgctgaatgcctgaccaaacggagaagcaaacgtggtccatgaacaacgtatcagagacttttagacaagagagaaatcacattctaaggggttttttcccccttttttttgagtggaagagttttttttattgagaggtatcgagaagtactatatttttcgtgtaagctagccaaatatcagtgcgttgcaacttgcaacggattattaaaaatagcaaaattaagtccttgttaaattttttttaaaaaaaaatggttgaaacacaacttttcatcttaaagaaacaaacaaaaatgccatgcttagaaaattaaagagctaaagtatagtacagtatatatagtacaggcgactctcccaaaccttaataccatggcccatatatcagtgacccaggccctgggcaaactggtccacggcccagggcctgagatgaaaggcccgatgagaaatatgcatttttactaggaataagtctatttttcctcctttcaaccatgtaactttgtgttgggccaaaatttccatggggcggcctaggccttggcctatttctgggtccgccccttatcagtgattgtggtgatggcagattcactaccaccactactatcttttaatagtagtaaagattttggtgtcatcttgtaattttaagatgtacaagacggtaccataaattgactttaaagtgatttgaatggatgtgtaatgggagaaatcgcttttcaaatgtaaaataatgcactacttgtattagtactaaattttacattagagaacacgatatattcagtgattcttgataaccataaaatccctttaagattatggtgttttctttaagtactgttaaaaaaaaaccaatagtgtctactggttacactgcagaagaagagaaccctgcagactgcagtatttgtaattgagcaagcagatacatatacctaaatgcatatatgttcattacctacaattcctaacgttgttgcagccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtaagtacttcatcaggctcttaataatacttccttcttccttaaatgtttgacgccgttgacttttttaaacatgtttgaccgttcgtcttattcaaaaacttttgtgaaatgtgtaaaactatatgtatacataaaagtatatttaacaataaatcaaatgatagaaaaagaattaataattatttaaatttttgaataagacgaacggtcaaatatttttaaaaaagtcaatggcatcaaatattttgggatggagggagtatgtaaaagagtaactttacagtttttaagaaagcataggggaagtaccatattttatattggagaaattttacggttttcagtatttcttttaagataaaggaggctttattgatcttggatacaatatactctaaaaccactcctgatatctgcatagctaggatgcacatggtctatagttattgaaaaagccaaataaaagaagatgatgtgcatgtcactctgcaaaataaataacttgatcatacatgctgttttcactgttataataatctgaacttatgtgatatattaggtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccatgtaagagtaaccatgttcagtgcatgattcacagatggtgtgcatgataaattgataatcaggttagaaacattttgttttgtgcagtatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggtagctcatttcctaatgaattcagagcaagttgaaacattatcatccatacttcagaattcagatgttagctatatgtccacatgtactgccaaattttagtcaagataaaacagttagtgcttaataattgtttatcttcagggaaaaggtttagtcataaaggtaggaaaataaaaaaattaagtagcaaagagtccgttgacagtagtccaacagaactaataaattctgtacatttcatgagaatacaatatgtagaaattagggaagtatgtactcatgcatggttgaactggtaattgacatacaatatcttcaatataaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtaattaatccactactcacctctgatttattttatagcatgcaaaaggataccatgttgtaacaaaccactattcttgtacaggtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtaagaatagatttcctcatttttcctacattctctggacaaatgaatagaatgtataagagaaatatcattgcctaataaaccaaaaattttcaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgaggtagtagtaataacctgttgaaataatccagagattcagacgaacttcacatgatctaatcacaaaatttttgaactttttttttgacagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttgatgattcagttgcaaagtatcagaataagttttagctggattatgctatgtggtcaccgtatcagaaagagagatgttctgttggcaagaagagatatagtatctgagtaatttcatctgccatggtttttaaaacattgttcatcatctcctttgatcaaagactggccatggctactttctgtataagtatctgtcctttttagtccatagtgtaattcagcaacaaaacactcttggcattgttatgcagtctcatgaatgtttttccagcatacggaattacgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071361.1 RefSeq:Os10g0471100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184058 2014-06-14T04:12:04Z

<p>DYM: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is strongly expressed in the epidermal cells of rice anthers. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. The wda1 mutant exhibits developmental and<br /> biochemical phenotypes that reveal the functioning of Wda1 in the epidermal layer and tapetum of the anther wall, the orbicule in the<br /> peritapetal region and pollen development, and the biosynthesis of VLCFA derivatives required for cuticle formation and wax deposition. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> Wda1 Is Preferentially Expressed in Epidermis of Various Floral Organs, including Anthers.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0471100|<br /> Description = Sterol desaturase family protein|<br /> Version = NM_001071361.1 GI:115482465 GeneID:4348869|<br /> Length = 5861 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0471100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:17906207..17912067|<br /> CDS = 17906494..17906655,17906746..17906922,17907019..17907117,17907202..17907402,17907731..17907838&lt;br&gt;,17907926..17908307,17908870..17909089,17910772..17911004,17911137..17911360&lt;br&gt;,17911825..17911884|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactgggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccattatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATNPGLFTEWPWKKLGSFKYVLLAPWVAHGWYEVATKGWREVD LGYIAILPSLLLRMLHNQAWITISRLQNARGRRQIVRRGIEFDQVDRERNWDDQIILS GILLYLGALYVPGGQHLPLWRTDGAGLIALLHAGPVEFLYYWFHRALHHHFLYTHYHS HHHSSIVTEPITSVIHPFAELVAYELLFSIPLIACALTGTASIIAFEMYLIYIDFMNN MGHCNFELVPSWLFTWFPPLKYLMYTPSFHSLHHTQFRTNYSLFMPFYDYIYNTMDKS SDTLYENSLKNNEEEEAVDVVHLTHLTTLHSIYHMRPGFAEFASRPYVSRWYMRMMWP LSWLSMVLTWTYGSSFTVERNVMKKIRMQSWAIPRYSFHYGLDWEKEAINDLIEKAVC EADKNGAKVVSLGLLNQAHTLNKSGEQYLLKYPKLGARIVDGTSLAAAVVVNSIPQGT DQVILAGNVSKVARAVAQALCKKNIKVTMTNKQDYHLLKPEIPETVADNLSFSKTGTA KVWLIGDGLDSAEQFRAQKGTLFIPYSQFPPKMVRKDSCSYSTTPAMAVPKTLQNVHS CENWLPRRVMSAWRIAGILHALEGWNEHECGDKVLDMDKVWSAAIMHGFCPVAQG&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5413..5574#5146..5322#4951..5049#4666..4866#4230..4337#3761..4142#2979..3198#1064..1296#708..931#184..243#atatgagactctgacacgtacgcacagctagatcgaagccctggtcgagtttataaattgagatatatccgtcctccattctcaggccagttcttgcaagactcttgccgtgttgagactgcagaaacgagagctagctagccagaggctgcaagaagattctgcacccatagatcgtctaccatggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaaggtaatcaaaattaaccttccatcttcttctccttaattcagttacttcactgtcttctggcagctcgatcgagcaaataaacgcacagcttctagttccatccatggcagcactgcaccatgaccattatgttcttgtttgagttcgctagcttctgtttgatatcaaagcatacattgccgcatgtgcagctagcagtgcatgagattaattctcttgcatctgctgcagatcagtaatgcaggggcatgaactttcctgcagcacctctgtgcagcaaataaactgttaaagagtacatggttaaataagcttttgccacatcgtctttgattaggcaaaaacattcagtatacacaacttccgacctttacaaattacaacagtaacttcagctataaattatagcacatgtgagttaattcgatggatggattcatgcatgtatatgtgtgcatatgcagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactggtatgccaaacaccataaaattacgtacaacccacgcggtgtgttcgtggcggctagcttcaccttcgagattaattaattcaacctcattatagttgatgtgttgttacgtgtatggtggtgtgcgtgcagggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatgtacgtaattaattgccactagagacggtttatcctcgaggctccaaacattacattctcttttaggcaataacctttgagcttctatattagtatcgaattgtgttgcgtcataaatatttttacaaaatttttattcatcatctaaagttatcaatgatcaaagttcaatcaatactaatagttacaagtaatttgaaatagaggaagtaagttgtactacctagttgtaccatcattgctccaaacaacctattacatgatattactttcgtcccaaacctctaaatgaatattaagattttggtaatacaaattcttaaaattttgagacagaaatattactagacctgtaaccttgggataaattaaaccacggtaatcttccaatagaacaaagtaaaaaacaagtcttttccataaggacacaaaacaggaggtgtacttcgtcagatgcaagtgtcgcgcgtgtccgttcctcaggaaacacagagtgttcctgtggccagtcgatcgctacttcaattccctcctccttcaatgcatgcctgaacaataattgtaggcctagagagtgtgtgagttaaggaatgcaaaggacacacgatatttcatggaccattgagatcgaagtaagccgaccgatcgatcgcgagctaaatttatgtagcccatcgaatccgttgctgaatgcctgaccaaacggagaagcaaacgtggtccatgaacaacgtatcagagacttttagacaagagagaaatcacattctaaggggttttttcccccttttttttgagtggaagagttttttttattgagaggtatcgagaagtactatatttttcgtgtaagctagccaaatatcagtgcgttgcaacttgcaacggattattaaaaatagcaaaattaagtccttgttaaattttttttaaaaaaaaatggttgaaacacaacttttcatcttaaagaaacaaacaaaaatgccatgcttagaaaattaaagagctaaagtatagtacagtatatatagtacaggcgactctcccaaaccttaataccatggcccatatatcagtgacccaggccctgggcaaactggtccacggcccagggcctgagatgaaaggcccgatgagaaatatgcatttttactaggaataagtctatttttcctcctttcaaccatgtaactttgtgttgggccaaaatttccatggggcggcctaggccttggcctatttctgggtccgccccttatcagtgattgtggtgatggcagattcactaccaccactactatcttttaatagtagtaaagattttggtgtcatcttgtaattttaagatgtacaagacggtaccataaattgactttaaagtgatttgaatggatgtgtaatgggagaaatcgcttttcaaatgtaaaataatgcactacttgtattagtactaaattttacattagagaacacgatatattcagtgattcttgataaccataaaatccctttaagattatggtgttttctttaagtactgttaaaaaaaaaccaatagtgtctactggttacactgcagaagaagagaaccctgcagactgcagtatttgtaattgagcaagcagatacatatacctaaatgcatatatgttcattacctacaattcctaacgttgttgcagccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtaagtacttcatcaggctcttaataatacttccttcttccttaaatgtttgacgccgttgacttttttaaacatgtttgaccgttcgtcttattcaaaaacttttgtgaaatgtgtaaaactatatgtatacataaaagtatatttaacaataaatcaaatgatagaaaaagaattaataattatttaaatttttgaataagacgaacggtcaaatatttttaaaaaagtcaatggcatcaaatattttgggatggagggagtatgtaaaagagtaactttacagtttttaagaaagcataggggaagtaccatattttatattggagaaattttacggttttcagtatttcttttaagataaaggaggctttattgatcttggatacaatatactctaaaaccactcctgatatctgcatagctaggatgcacatggtctatagttattgaaaaagccaaataaaagaagatgatgtgcatgtcactctgcaaaataaataacttgatcatacatgctgttttcactgttataataatctgaacttatgtgatatattaggtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccatgtaagagtaaccatgttcagtgcatgattcacagatggtgtgcatgataaattgataatcaggttagaaacattttgttttgtgcagtatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggtagctcatttcctaatgaattcagagcaagttgaaacattatcatccatacttcagaattcagatgttagctatatgtccacatgtactgccaaattttagtcaagataaaacagttagtgcttaataattgtttatcttcagggaaaaggtttagtcataaaggtaggaaaataaaaaaattaagtagcaaagagtccgttgacagtagtccaacagaactaataaattctgtacatttcatgagaatacaatatgtagaaattagggaagtatgtactcatgcatggttgaactggtaattgacatacaatatcttcaatataaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtaattaatccactactcacctctgatttattttatagcatgcaaaaggataccatgttgtaacaaaccactattcttgtacaggtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtaagaatagatttcctcatttttcctacattctctggacaaatgaatagaatgtataagagaaatatcattgcctaataaaccaaaaattttcaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgaggtagtagtaataacctgttgaaataatccagagattcagacgaacttcacatgatctaatcacaaaatttttgaactttttttttgacagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttgatgattcagttgcaaagtatcagaataagttttagctggattatgctatgtggtcaccgtatcagaaagagagatgttctgttggcaagaagagatatagtatctgagtaatttcatctgccatggtttttaaaacattgttcatcatctcctttgatcaaagactggccatggctactttctgtataagtatctgtcctttttagtccatagtgtaattcagcaacaaaacactcttggcattgttatgcagtctcatgaatgtttttccagcatacggaattacgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071361.1 RefSeq:Os10g0471100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184057 2014-06-14T04:10:24Z

<p>DYM: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is strongly expressed in the epidermal cells of rice anthers. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. The wda1 mutant exhibits developmental and<br /> biochemical phenotypes that reveal the functioning of Wda1 in the epidermal layer and tapetum of the anther wall, the orbicule in the<br /> peritapetal region and pollen development, and the biosynthesis of VLCFA derivatives required for cuticle formation and wax deposition. Scanning electron microscopy analysis shows that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0471100|<br /> Description = Sterol desaturase family protein|<br /> Version = NM_001071361.1 GI:115482465 GeneID:4348869|<br /> Length = 5861 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0471100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:17906207..17912067|<br /> CDS = 17906494..17906655,17906746..17906922,17907019..17907117,17907202..17907402,17907731..17907838&lt;br&gt;,17907926..17908307,17908870..17909089,17910772..17911004,17911137..17911360&lt;br&gt;,17911825..17911884|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactgggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccattatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATNPGLFTEWPWKKLGSFKYVLLAPWVAHGWYEVATKGWREVD LGYIAILPSLLLRMLHNQAWITISRLQNARGRRQIVRRGIEFDQVDRERNWDDQIILS GILLYLGALYVPGGQHLPLWRTDGAGLIALLHAGPVEFLYYWFHRALHHHFLYTHYHS HHHSSIVTEPITSVIHPFAELVAYELLFSIPLIACALTGTASIIAFEMYLIYIDFMNN MGHCNFELVPSWLFTWFPPLKYLMYTPSFHSLHHTQFRTNYSLFMPFYDYIYNTMDKS SDTLYENSLKNNEEEEAVDVVHLTHLTTLHSIYHMRPGFAEFASRPYVSRWYMRMMWP LSWLSMVLTWTYGSSFTVERNVMKKIRMQSWAIPRYSFHYGLDWEKEAINDLIEKAVC EADKNGAKVVSLGLLNQAHTLNKSGEQYLLKYPKLGARIVDGTSLAAAVVVNSIPQGT DQVILAGNVSKVARAVAQALCKKNIKVTMTNKQDYHLLKPEIPETVADNLSFSKTGTA KVWLIGDGLDSAEQFRAQKGTLFIPYSQFPPKMVRKDSCSYSTTPAMAVPKTLQNVHS CENWLPRRVMSAWRIAGILHALEGWNEHECGDKVLDMDKVWSAAIMHGFCPVAQG&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5413..5574#5146..5322#4951..5049#4666..4866#4230..4337#3761..4142#2979..3198#1064..1296#708..931#184..243#atatgagactctgacacgtacgcacagctagatcgaagccctggtcgagtttataaattgagatatatccgtcctccattctcaggccagttcttgcaagactcttgccgtgttgagactgcagaaacgagagctagctagccagaggctgcaagaagattctgcacccatagatcgtctaccatggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaaggtaatcaaaattaaccttccatcttcttctccttaattcagttacttcactgtcttctggcagctcgatcgagcaaataaacgcacagcttctagttccatccatggcagcactgcaccatgaccattatgttcttgtttgagttcgctagcttctgtttgatatcaaagcatacattgccgcatgtgcagctagcagtgcatgagattaattctcttgcatctgctgcagatcagtaatgcaggggcatgaactttcctgcagcacctctgtgcagcaaataaactgttaaagagtacatggttaaataagcttttgccacatcgtctttgattaggcaaaaacattcagtatacacaacttccgacctttacaaattacaacagtaacttcagctataaattatagcacatgtgagttaattcgatggatggattcatgcatgtatatgtgtgcatatgcagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactggtatgccaaacaccataaaattacgtacaacccacgcggtgtgttcgtggcggctagcttcaccttcgagattaattaattcaacctcattatagttgatgtgttgttacgtgtatggtggtgtgcgtgcagggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatgtacgtaattaattgccactagagacggtttatcctcgaggctccaaacattacattctcttttaggcaataacctttgagcttctatattagtatcgaattgtgttgcgtcataaatatttttacaaaatttttattcatcatctaaagttatcaatgatcaaagttcaatcaatactaatagttacaagtaatttgaaatagaggaagtaagttgtactacctagttgtaccatcattgctccaaacaacctattacatgatattactttcgtcccaaacctctaaatgaatattaagattttggtaatacaaattcttaaaattttgagacagaaatattactagacctgtaaccttgggataaattaaaccacggtaatcttccaatagaacaaagtaaaaaacaagtcttttccataaggacacaaaacaggaggtgtacttcgtcagatgcaagtgtcgcgcgtgtccgttcctcaggaaacacagagtgttcctgtggccagtcgatcgctacttcaattccctcctccttcaatgcatgcctgaacaataattgtaggcctagagagtgtgtgagttaaggaatgcaaaggacacacgatatttcatggaccattgagatcgaagtaagccgaccgatcgatcgcgagctaaatttatgtagcccatcgaatccgttgctgaatgcctgaccaaacggagaagcaaacgtggtccatgaacaacgtatcagagacttttagacaagagagaaatcacattctaaggggttttttcccccttttttttgagtggaagagttttttttattgagaggtatcgagaagtactatatttttcgtgtaagctagccaaatatcagtgcgttgcaacttgcaacggattattaaaaatagcaaaattaagtccttgttaaattttttttaaaaaaaaatggttgaaacacaacttttcatcttaaagaaacaaacaaaaatgccatgcttagaaaattaaagagctaaagtatagtacagtatatatagtacaggcgactctcccaaaccttaataccatggcccatatatcagtgacccaggccctgggcaaactggtccacggcccagggcctgagatgaaaggcccgatgagaaatatgcatttttactaggaataagtctatttttcctcctttcaaccatgtaactttgtgttgggccaaaatttccatggggcggcctaggccttggcctatttctgggtccgccccttatcagtgattgtggtgatggcagattcactaccaccactactatcttttaatagtagtaaagattttggtgtcatcttgtaattttaagatgtacaagacggtaccataaattgactttaaagtgatttgaatggatgtgtaatgggagaaatcgcttttcaaatgtaaaataatgcactacttgtattagtactaaattttacattagagaacacgatatattcagtgattcttgataaccataaaatccctttaagattatggtgttttctttaagtactgttaaaaaaaaaccaatagtgtctactggttacactgcagaagaagagaaccctgcagactgcagtatttgtaattgagcaagcagatacatatacctaaatgcatatatgttcattacctacaattcctaacgttgttgcagccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtaagtacttcatcaggctcttaataatacttccttcttccttaaatgtttgacgccgttgacttttttaaacatgtttgaccgttcgtcttattcaaaaacttttgtgaaatgtgtaaaactatatgtatacataaaagtatatttaacaataaatcaaatgatagaaaaagaattaataattatttaaatttttgaataagacgaacggtcaaatatttttaaaaaagtcaatggcatcaaatattttgggatggagggagtatgtaaaagagtaactttacagtttttaagaaagcataggggaagtaccatattttatattggagaaattttacggttttcagtatttcttttaagataaaggaggctttattgatcttggatacaatatactctaaaaccactcctgatatctgcatagctaggatgcacatggtctatagttattgaaaaagccaaataaaagaagatgatgtgcatgtcactctgcaaaataaataacttgatcatacatgctgttttcactgttataataatctgaacttatgtgatatattaggtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccatgtaagagtaaccatgttcagtgcatgattcacagatggtgtgcatgataaattgataatcaggttagaaacattttgttttgtgcagtatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggtagctcatttcctaatgaattcagagcaagttgaaacattatcatccatacttcagaattcagatgttagctatatgtccacatgtactgccaaattttagtcaagataaaacagttagtgcttaataattgtttatcttcagggaaaaggtttagtcataaaggtaggaaaataaaaaaattaagtagcaaagagtccgttgacagtagtccaacagaactaataaattctgtacatttcatgagaatacaatatgtagaaattagggaagtatgtactcatgcatggttgaactggtaattgacatacaatatcttcaatataaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtaattaatccactactcacctctgatttattttatagcatgcaaaaggataccatgttgtaacaaaccactattcttgtacaggtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtaagaatagatttcctcatttttcctacattctctggacaaatgaatagaatgtataagagaaatatcattgcctaataaaccaaaaattttcaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgaggtagtagtaataacctgttgaaataatccagagattcagacgaacttcacatgatctaatcacaaaatttttgaactttttttttgacagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttgatgattcagttgcaaagtatcagaataagttttagctggattatgctatgtggtcaccgtatcagaaagagagatgttctgttggcaagaagagatatagtatctgagtaatttcatctgccatggtttttaaaacattgttcatcatctcctttgatcaaagactggccatggctactttctgtataagtatctgtcctttttagtccatagtgtaattcagcaacaaaacactcttggcattgttatgcagtctcatgaatgtttttccagcatacggaattacgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071361.1 RefSeq:Os10g0471100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184056 2014-06-14T03:49:45Z

<p>DYM: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is strongly expressed in the epidermal cells of rice anthers. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular waxx crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced.<br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0471100|<br /> Description = Sterol desaturase family protein|<br /> Version = NM_001071361.1 GI:115482465 GeneID:4348869|<br /> Length = 5861 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0471100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:17906207..17912067|<br /> CDS = 17906494..17906655,17906746..17906922,17907019..17907117,17907202..17907402,17907731..17907838&lt;br&gt;,17907926..17908307,17908870..17909089,17910772..17911004,17911137..17911360&lt;br&gt;,17911825..17911884|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactgggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccattatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATNPGLFTEWPWKKLGSFKYVLLAPWVAHGWYEVATKGWREVD LGYIAILPSLLLRMLHNQAWITISRLQNARGRRQIVRRGIEFDQVDRERNWDDQIILS GILLYLGALYVPGGQHLPLWRTDGAGLIALLHAGPVEFLYYWFHRALHHHFLYTHYHS HHHSSIVTEPITSVIHPFAELVAYELLFSIPLIACALTGTASIIAFEMYLIYIDFMNN MGHCNFELVPSWLFTWFPPLKYLMYTPSFHSLHHTQFRTNYSLFMPFYDYIYNTMDKS SDTLYENSLKNNEEEEAVDVVHLTHLTTLHSIYHMRPGFAEFASRPYVSRWYMRMMWP LSWLSMVLTWTYGSSFTVERNVMKKIRMQSWAIPRYSFHYGLDWEKEAINDLIEKAVC EADKNGAKVVSLGLLNQAHTLNKSGEQYLLKYPKLGARIVDGTSLAAAVVVNSIPQGT DQVILAGNVSKVARAVAQALCKKNIKVTMTNKQDYHLLKPEIPETVADNLSFSKTGTA KVWLIGDGLDSAEQFRAQKGTLFIPYSQFPPKMVRKDSCSYSTTPAMAVPKTLQNVHS CENWLPRRVMSAWRIAGILHALEGWNEHECGDKVLDMDKVWSAAIMHGFCPVAQG&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5413..5574#5146..5322#4951..5049#4666..4866#4230..4337#3761..4142#2979..3198#1064..1296#708..931#184..243#atatgagactctgacacgtacgcacagctagatcgaagccctggtcgagtttataaattgagatatatccgtcctccattctcaggccagttcttgcaagactcttgccgtgttgagactgcagaaacgagagctagctagccagaggctgcaagaagattctgcacccatagatcgtctaccatggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaaggtaatcaaaattaaccttccatcttcttctccttaattcagttacttcactgtcttctggcagctcgatcgagcaaataaacgcacagcttctagttccatccatggcagcactgcaccatgaccattatgttcttgtttgagttcgctagcttctgtttgatatcaaagcatacattgccgcatgtgcagctagcagtgcatgagattaattctcttgcatctgctgcagatcagtaatgcaggggcatgaactttcctgcagcacctctgtgcagcaaataaactgttaaagagtacatggttaaataagcttttgccacatcgtctttgattaggcaaaaacattcagtatacacaacttccgacctttacaaattacaacagtaacttcagctataaattatagcacatgtgagttaattcgatggatggattcatgcatgtatatgtgtgcatatgcagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactggtatgccaaacaccataaaattacgtacaacccacgcggtgtgttcgtggcggctagcttcaccttcgagattaattaattcaacctcattatagttgatgtgttgttacgtgtatggtggtgtgcgtgcagggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatgtacgtaattaattgccactagagacggtttatcctcgaggctccaaacattacattctcttttaggcaataacctttgagcttctatattagtatcgaattgtgttgcgtcataaatatttttacaaaatttttattcatcatctaaagttatcaatgatcaaagttcaatcaatactaatagttacaagtaatttgaaatagaggaagtaagttgtactacctagttgtaccatcattgctccaaacaacctattacatgatattactttcgtcccaaacctctaaatgaatattaagattttggtaatacaaattcttaaaattttgagacagaaatattactagacctgtaaccttgggataaattaaaccacggtaatcttccaatagaacaaagtaaaaaacaagtcttttccataaggacacaaaacaggaggtgtacttcgtcagatgcaagtgtcgcgcgtgtccgttcctcaggaaacacagagtgttcctgtggccagtcgatcgctacttcaattccctcctccttcaatgcatgcctgaacaataattgtaggcctagagagtgtgtgagttaaggaatgcaaaggacacacgatatttcatggaccattgagatcgaagtaagccgaccgatcgatcgcgagctaaatttatgtagcccatcgaatccgttgctgaatgcctgaccaaacggagaagcaaacgtggtccatgaacaacgtatcagagacttttagacaagagagaaatcacattctaaggggttttttcccccttttttttgagtggaagagttttttttattgagaggtatcgagaagtactatatttttcgtgtaagctagccaaatatcagtgcgttgcaacttgcaacggattattaaaaatagcaaaattaagtccttgttaaattttttttaaaaaaaaatggttgaaacacaacttttcatcttaaagaaacaaacaaaaatgccatgcttagaaaattaaagagctaaagtatagtacagtatatatagtacaggcgactctcccaaaccttaataccatggcccatatatcagtgacccaggccctgggcaaactggtccacggcccagggcctgagatgaaaggcccgatgagaaatatgcatttttactaggaataagtctatttttcctcctttcaaccatgtaactttgtgttgggccaaaatttccatggggcggcctaggccttggcctatttctgggtccgccccttatcagtgattgtggtgatggcagattcactaccaccactactatcttttaatagtagtaaagattttggtgtcatcttgtaattttaagatgtacaagacggtaccataaattgactttaaagtgatttgaatggatgtgtaatgggagaaatcgcttttcaaatgtaaaataatgcactacttgtattagtactaaattttacattagagaacacgatatattcagtgattcttgataaccataaaatccctttaagattatggtgttttctttaagtactgttaaaaaaaaaccaatagtgtctactggttacactgcagaagaagagaaccctgcagactgcagtatttgtaattgagcaagcagatacatatacctaaatgcatatatgttcattacctacaattcctaacgttgttgcagccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtaagtacttcatcaggctcttaataatacttccttcttccttaaatgtttgacgccgttgacttttttaaacatgtttgaccgttcgtcttattcaaaaacttttgtgaaatgtgtaaaactatatgtatacataaaagtatatttaacaataaatcaaatgatagaaaaagaattaataattatttaaatttttgaataagacgaacggtcaaatatttttaaaaaagtcaatggcatcaaatattttgggatggagggagtatgtaaaagagtaactttacagtttttaagaaagcataggggaagtaccatattttatattggagaaattttacggttttcagtatttcttttaagataaaggaggctttattgatcttggatacaatatactctaaaaccactcctgatatctgcatagctaggatgcacatggtctatagttattgaaaaagccaaataaaagaagatgatgtgcatgtcactctgcaaaataaataacttgatcatacatgctgttttcactgttataataatctgaacttatgtgatatattaggtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccatgtaagagtaaccatgttcagtgcatgattcacagatggtgtgcatgataaattgataatcaggttagaaacattttgttttgtgcagtatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggtagctcatttcctaatgaattcagagcaagttgaaacattatcatccatacttcagaattcagatgttagctatatgtccacatgtactgccaaattttagtcaagataaaacagttagtgcttaataattgtttatcttcagggaaaaggtttagtcataaaggtaggaaaataaaaaaattaagtagcaaagagtccgttgacagtagtccaacagaactaataaattctgtacatttcatgagaatacaatatgtagaaattagggaagtatgtactcatgcatggttgaactggtaattgacatacaatatcttcaatataaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtaattaatccactactcacctctgatttattttatagcatgcaaaaggataccatgttgtaacaaaccactattcttgtacaggtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtaagaatagatttcctcatttttcctacattctctggacaaatgaatagaatgtataagagaaatatcattgcctaataaaccaaaaattttcaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgaggtagtagtaataacctgttgaaataatccagagattcagacgaacttcacatgatctaatcacaaaatttttgaactttttttttgacagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttgatgattcagttgcaaagtatcagaataagttttagctggattatgctatgtggtcaccgtatcagaaagagagatgttctgttggcaagaagagatatagtatctgagtaatttcatctgccatggtttttaaaacattgttcatcatctcctttgatcaaagactggccatggctactttctgtataagtatctgtcctttttagtccatagtgtaattcagcaacaaaacactcttggcattgttatgcagtctcatgaatgtttttccagcatacggaattacgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071361.1 RefSeq:Os10g0471100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div>

DYM https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0471100&diff=184055 2014-06-14T03:49:11Z

<p>DYM: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> Wax-deficient anther1 (Wda1), a rice homolog of Arabidopsis CER1, is strongly expressed in the epidermal cells of rice anthers. Wda1 mutant shows defects in the biosynthesis of very-long-chain fatty acids in both layers of anthers. Scanning electron microscopy analysis shows that epicuticular waxx crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mature anthers. Biochemical analysis of the wda1 mutant anthers showed that the levels of very-long-chain alkanes, alkens, fatty acids, and primary alchols are severely reduced.<br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0471100|<br /> Description = Sterol desaturase family protein|<br /> Version = NM_001071361.1 GI:115482465 GeneID:4348869|<br /> Length = 5861 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0471100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:17906207..17912067|<br /> CDS = 17906494..17906655,17906746..17906922,17907019..17907117,17907202..17907402,17907731..17907838&lt;br&gt;,17907926..17908307,17908870..17909089,17910772..17911004,17911137..17911360&lt;br&gt;,17911825..17911884|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:17906207..17912067<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactgggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccattatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATNPGLFTEWPWKKLGSFKYVLLAPWVAHGWYEVATKGWREVD LGYIAILPSLLLRMLHNQAWITISRLQNARGRRQIVRRGIEFDQVDRERNWDDQIILS GILLYLGALYVPGGQHLPLWRTDGAGLIALLHAGPVEFLYYWFHRALHHHFLYTHYHS HHHSSIVTEPITSVIHPFAELVAYELLFSIPLIACALTGTASIIAFEMYLIYIDFMNN MGHCNFELVPSWLFTWFPPLKYLMYTPSFHSLHHTQFRTNYSLFMPFYDYIYNTMDKS SDTLYENSLKNNEEEEAVDVVHLTHLTTLHSIYHMRPGFAEFASRPYVSRWYMRMMWP LSWLSMVLTWTYGSSFTVERNVMKKIRMQSWAIPRYSFHYGLDWEKEAINDLIEKAVC EADKNGAKVVSLGLLNQAHTLNKSGEQYLLKYPKLGARIVDGTSLAAAVVVNSIPQGT DQVILAGNVSKVARAVAQALCKKNIKVTMTNKQDYHLLKPEIPETVADNLSFSKTGTA KVWLIGDGLDSAEQFRAQKGTLFIPYSQFPPKMVRKDSCSYSTTPAMAVPKTLQNVHS CENWLPRRVMSAWRIAGILHALEGWNEHECGDKVLDMDKVWSAAIMHGFCPVAQG&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5413..5574#5146..5322#4951..5049#4666..4866#4230..4337#3761..4142#2979..3198#1064..1296#708..931#184..243#atatgagactctgacacgtacgcacagctagatcgaagccctggtcgagtttataaattgagatatatccgtcctccattctcaggccagttcttgcaagactcttgccgtgttgagactgcagaaacgagagctagctagccagaggctgcaagaagattctgcacccatagatcgtctaccatggccacaaacccaggactcttcacggagtggccatggaagaagcttggcagcttcaaggtaatcaaaattaaccttccatcttcttctccttaattcagttacttcactgtcttctggcagctcgatcgagcaaataaacgcacagcttctagttccatccatggcagcactgcaccatgaccattatgttcttgtttgagttcgctagcttctgtttgatatcaaagcatacattgccgcatgtgcagctagcagtgcatgagattaattctcttgcatctgctgcagatcagtaatgcaggggcatgaactttcctgcagcacctctgtgcagcaaataaactgttaaagagtacatggttaaataagcttttgccacatcgtctttgattaggcaaaaacattcagtatacacaacttccgacctttacaaattacaacagtaacttcagctataaattatagcacatgtgagttaattcgatggatggattcatgcatgtatatgtgtgcatatgcagtacgtgttgctggcgccgtgggtagcgcacgggtggtatgaggtggcgaccaaggggtggcgggaggtggatctcgggtacatcgccatcctcccgtcgttgctgctgcggatgctgcacaaccaagcctggatcaccatctcccgcctccaaaacgcccgtggccgccgccagattgttcgccgcggcatcgagttcgaccaggttgaccgcgagaggaactggtatgccaaacaccataaaattacgtacaacccacgcggtgtgttcgtggcggctagcttcaccttcgagattaattaattcaacctcattatagttgatgtgttgttacgtgtatggtggtgtgcgtgcagggacgaccagatcatcctgagcggtatcctactatacctcggcgcactgtacgtaccgggcgggcaacacttaccgctgtggaggacggatggcgcggggctgatcgccttgctgcatgcggggccagtggagttcctctactactggttccatcgtgcgttgcaccaccactttctctacacccactaccactcgcaccaccactcttcaatcgtcactgagcccatcacatgtacgtaattaattgccactagagacggtttatcctcgaggctccaaacattacattctcttttaggcaataacctttgagcttctatattagtatcgaattgtgttgcgtcataaatatttttacaaaatttttattcatcatctaaagttatcaatgatcaaagttcaatcaatactaatagttacaagtaatttgaaatagaggaagtaagttgtactacctagttgtaccatcattgctccaaacaacctattacatgatattactttcgtcccaaacctctaaatgaatattaagattttggtaatacaaattcttaaaattttgagacagaaatattactagacctgtaaccttgggataaattaaaccacggtaatcttccaatagaacaaagtaaaaaacaagtcttttccataaggacacaaaacaggaggtgtacttcgtcagatgcaagtgtcgcgcgtgtccgttcctcaggaaacacagagtgttcctgtggccagtcgatcgctacttcaattccctcctccttcaatgcatgcctgaacaataattgtaggcctagagagtgtgtgagttaaggaatgcaaaggacacacgatatttcatggaccattgagatcgaagtaagccgaccgatcgatcgcgagctaaatttatgtagcccatcgaatccgttgctgaatgcctgaccaaacggagaagcaaacgtggtccatgaacaacgtatcagagacttttagacaagagagaaatcacattctaaggggttttttcccccttttttttgagtggaagagttttttttattgagaggtatcgagaagtactatatttttcgtgtaagctagccaaatatcagtgcgttgcaacttgcaacggattattaaaaatagcaaaattaagtccttgttaaattttttttaaaaaaaaatggttgaaacacaacttttcatcttaaagaaacaaacaaaaatgccatgcttagaaaattaaagagctaaagtatagtacagtatatatagtacaggcgactctcccaaaccttaataccatggcccatatatcagtgacccaggccctgggcaaactggtccacggcccagggcctgagatgaaaggcccgatgagaaatatgcatttttactaggaataagtctatttttcctcctttcaaccatgtaactttgtgttgggccaaaatttccatggggcggcctaggccttggcctatttctgggtccgccccttatcagtgattgtggtgatggcagattcactaccaccactactatcttttaatagtagtaaagattttggtgtcatcttgtaattttaagatgtacaagacggtaccataaattgactttaaagtgatttgaatggatgtgtaatgggagaaatcgcttttcaaatgtaaaataatgcactacttgtattagtactaaattttacattagagaacacgatatattcagtgattcttgataaccataaaatccctttaagattatggtgttttctttaagtactgttaaaaaaaaaccaatagtgtctactggttacactgcagaagaagagaaccctgcagactgcagtatttgtaattgagcaagcagatacatatacctaaatgcatatatgttcattacctacaattcctaacgttgttgcagccgtcatccatccgtttgctgagctcgttgcctacgaattgctcttctcgatcccactgatcgcctgtgccttgactggaactgcttccataattgcattcgagatgtatttgatttacattgatttcatgaacaacatggggcactgcaacttcgagttggtacccagctggctcttcacatggttccctcctctcaagtatctcatgtacacgccatcgtaagtacttcatcaggctcttaataatacttccttcttccttaaatgtttgacgccgttgacttttttaaacatgtttgaccgttcgtcttattcaaaaacttttgtgaaatgtgtaaaactatatgtatacataaaagtatatttaacaataaatcaaatgatagaaaaagaattaataattatttaaatttttgaataagacgaacggtcaaatatttttaaaaaagtcaatggcatcaaatattttgggatggagggagtatgtaaaagagtaactttacagtttttaagaaagcataggggaagtaccatattttatattggagaaattttacggttttcagtatttcttttaagataaaggaggctttattgatcttggatacaatatactctaaaaccactcctgatatctgcatagctaggatgcacatggtctatagttattgaaaaagccaaataaaagaagatgatgtgcatgtcactctgcaaaataaataacttgatcatacatgctgttttcactgttataataatctgaacttatgtgatatattaggtttcattctctgcaccacactcagtttcggacaaactactcccttttcatgccgttctacgattatatctacaacaccatggacaagtcatcagacactctgtatgagaactcactgaaaaacaatgaggaagaagaagcagtggatgtggttcaccttacacacctgaccaccctgcattccatctaccacatgaggcccggtttcgccgaatttgcgtccaggccctacgtttccaggtggtacatgaggatgatgtggcctctgtcatggctctccatggtgctgacatggacgtacggttcttcattcactgttgagaggaatgtcatgaagaagatcaggatgcagtcatgggccataccaagatacagtttccatgtaagagtaaccatgttcagtgcatgattcacagatggtgtgcatgataaattgataatcaggttagaaacattttgttttgtgcagtatggattggattgggagaaggaagctatcaatgatcttatcgaaaaggcggtatgtgaagctgacaagaatggggctaaagttgtaagccttggactcctgaatcaggtagctcatttcctaatgaattcagagcaagttgaaacattatcatccatacttcagaattcagatgttagctatatgtccacatgtactgccaaattttagtcaagataaaacagttagtgcttaataattgtttatcttcagggaaaaggtttagtcataaaggtaggaaaataaaaaaattaagtagcaaagagtccgttgacagtagtccaacagaactaataaattctgtacatttcatgagaatacaatatgtagaaattagggaagtatgtactcatgcatggttgaactggtaattgacatacaatatcttcaatataaggcacataccctgaataaaagtggagaacaatatctgctgaaatacccaaagttgggagcaagaattgtcgatggaaccagcttagctgctgcagtggttgtcaacagtatcccccaaggcacagaccaagtaattcttgcaggaaatgtttccaaggtggcgcgtgctgtagcgcaagcattatgcaagaaaaatatcaaagtaattaatccactactcacctctgatttattttatagcatgcaaaaggataccatgttgtaacaaaccactattcttgtacaggtcacgatgacaaacaagcaagattatcatttactcaagccagaaataccagaaactgtagctgacaatctttcgttttcgaaaacaggcaccgcaaaggtaagaatagatttcctcatttttcctacattctctggacaaatgaatagaatgtataagagaaatatcattgcctaataaaccaaaaattttcaggtttggttaattggtgatggtctagattctgctgaacaattcagagcacagaaaggaactctgtttatcccatactcacaatttcctccaaagatggtacgcaaggacagctgcagctactcgacaactcctgcaatggctgtaccaaaaacgctgcagaatgtgcattcatgcgaggtagtagtaataacctgttgaaataatccagagattcagacgaacttcacatgatctaatcacaaaatttttgaactttttttttgacagaattggctgccaaggagggttatgagcgcatggcgaatcgcgggaattcttcatgcgttggagggatggaatgagcatgaatgtggtgacaaggtgcttgatatggacaaagtttggtctgctgcaattatgcatgggttctgcccagttgctcaaggttgatgattcagttgcaaagtatcagaataagttttagctggattatgctatgtggtcaccgtatcagaaagagagatgttctgttggcaagaagagatatagtatctgagtaatttcatctgccatggtttttaaaacattgttcatcatctcctttgatcaaagactggccatggctactttctgtataagtatctgtcctttttagtccatagtgtaattcagcaacaaaacactcttggcattgttatgcagtctcatgaatgtttttccagcatacggaattacgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071361.1 RefSeq:Os10g0471100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div>

DYM