http://192.168.164.12:81/ricewiki/api.php?action=feedcontributions&feedformat=atom&user=Damonsong 2026-10-12T04:45:54Z User contributions MediaWiki 1.30.0 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172079 2014-05-25T04:02:32Z

<p>Damonsong: </p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> 1. Cross-species complementation test of OsRTHs in ''Arabidopsis''('''fig.1''').<br /> <br /> Functional analyses of plant RTHs in Arabidopsis. The seedling triple-response phenotype of ethylene-grown wild type (Col-0) and ''35S:OsRTH1'' transformation lines (A), (''ETR1'')4LOF and a mutant expressing ''35S:OsRTH1'' (B),'' etr1''-7 and a mutant expressing ''35S:OsRTH1'' (C), and ''etr1-2 rte1-2 ''and a mutant expressing ''35S:OsRTH1'' (D). (E) The seedling triple-response phenotype of the wild type (Col-0) expressing ''35S:OsRTH2'' and ''35S:OsRTH3.'' (F) Seedling triple-response phenotype of ''etr1-2 rte1-2 ''expressing ''35S:OsRTH2'' and'' OsRTH3''. Leaf senescence phenotype of the wild type (Col-0) (G) and ethylene-insensitive ''etr1-2'' (H); phenotype of wild type (Col-0), (''ETR1'')4LOF (J), ''etr1-2 rte1-2'' (K), and'' etr1-7'' (L) expressing ''35S:OsRTH1''. Air and ethylene indicate the phenotype of the same plants before and after the treatment, respectively. Chlorophyll a measurement (M) and SAG12 expression (N) of the wild type (Col-0), ''etr1-2'', and ''35S:OsRTH1'' transformants in the corresponding mutation background as indicated. Error bars indicate the standard error (SE) for the means of five measurements. RT-PCR, analysis of the mRNA level of corresponding transgenes at the translational level. a (air) and b (ethylene) indicate a statistically significant difference (a-0.01) between the wild type and mutant or transformation lines.<br /> <br /> <br /> <br /> '''Fig.1 expression in ''Arabidopsis'''''<br /> [[File:Fig.2 expression.png]]<br /> <br /> <br /> <br /> 2. GFP-fused OsRTHs are associated with the Golgi('''fig.2''').<br /> <br /> '''Fig. 2 GFP-fused OsRTHs location'''<br /> <br /> [[File:Fig. 3 GFP.png]]<br /> <br /> <br /> <br /> <br /> 3. OsRTH1 overexpression prevents ethylene-induced riceleaf senescence('''fig.3''').<br /> <br /> '''Fig. 3 OsRTH1 overexpression in rice'''<br /> [[File:Fig. 4 corn.png]]<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> <br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 4A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 4B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 4C).<br /> <br /> <br /> <br /> '''Fig. 4'''<br /> [[File:Fig.1_evolution.png]]<br /> <br /> ==Labs working on this gene==<br /> 1. National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai), Institute of Plant<br /> Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.<br /> <br /> 2. Plant Gene Research Center, National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese<br /> Academy of Sciences.<br /> <br /> ==References==<br /> 1. Greco M, Chiappetta A, Bruno L, et al. In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning[J]. Journal of experimental botany, 2012, 63(2): 695-709.<br /> <br /> 2. Wuriyanghan H, Zhang B, Cao W H, et al. The ethylene receptor ETR2 delays floral transition and affects starch accumulation in rice[J]. The Plant Cell Online, 2009, 21(5): 1473-1494.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172077 2014-05-25T03:59:37Z

<p>Damonsong: /* References */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> 1. Cross-species complementation test of OsRTHs in ''Arabidopsis''('''fig.2''').<br /> <br /> Functional analyses of plant RTHs in Arabidopsis. The seedling triple-response phenotype of ethylene-grown wild type (Col-0) and ''35S:OsRTH1'' transformation lines (A), (''ETR1'')4LOF and a mutant expressing ''35S:OsRTH1'' (B),'' etr1''-7 and a mutant expressing ''35S:OsRTH1'' (C), and ''etr1-2 rte1-2 ''and a mutant expressing ''35S:OsRTH1'' (D). (E) The seedling triple-response phenotype of the wild type (Col-0) expressing ''35S:OsRTH2'' and ''35S:OsRTH3.'' (F) Seedling triple-response phenotype of ''etr1-2 rte1-2 ''expressing ''35S:OsRTH2'' and'' OsRTH3''. Leaf senescence phenotype of the wild type (Col-0) (G) and ethylene-insensitive ''etr1-2'' (H); phenotype of wild type (Col-0), (''ETR1'')4LOF (J), ''etr1-2 rte1-2'' (K), and'' etr1-7'' (L) expressing ''35S:OsRTH1''. Air and ethylene indicate the phenotype of the same plants before and after the treatment, respectively. Chlorophyll a measurement (M) and SAG12 expression (N) of the wild type (Col-0), ''etr1-2'', and ''35S:OsRTH1'' transformants in the corresponding mutation background as indicated. Error bars indicate the standard error (SE) for the means of five measurements. RT-PCR, analysis of the mRNA level of corresponding transgenes at the translational level. a (air) and b (ethylene) indicate a statistically significant difference (a-0.01) between the wild type and mutant or transformation lines.<br /> <br /> <br /> <br /> '''Fig.2 expression in ''Arabidopsis'''''<br /> [[File:Fig.2 expression.png]]<br /> <br /> <br /> <br /> 2. GFP-fused OsRTHs are associated with the Golgi('''fig.3''').<br /> <br /> '''Fig. 3 GFP-fused OsRTHs location'''<br /> <br /> [[File:Fig. 3 GFP.png]]<br /> <br /> <br /> <br /> <br /> 3. OsRTH1 overexpression prevents ethylene-induced riceleaf senescence('''fig.4''').<br /> <br /> '''Fig. 4 OsRTH1 overexpression in rice'''<br /> [[File:Fig. 4 corn.png]]<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> <br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 1A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 1B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 1C).<br /> <br /> <br /> <br /> '''Fig. 1'''<br /> [[File:Fig.1_evolution.png]]<br /> <br /> ==Labs working on this gene==<br /> 1. National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai), Institute of Plant<br /> Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.<br /> <br /> 2. Plant Gene Research Center, National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese<br /> Academy of Sciences.<br /> <br /> ==References==<br /> 1. Greco M, Chiappetta A, Bruno L, et al. In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning[J]. Journal of experimental botany, 2012, 63(2): 695-709.<br /> <br /> 2. Wuriyanghan H, Zhang B, Cao W H, et al. The ethylene receptor ETR2 delays floral transition and affects starch accumulation in rice[J]. The Plant Cell Online, 2009, 21(5): 1473-1494.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172076 2014-05-25T03:58:26Z

<p>Damonsong: /* Labs working on this gene */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> 1. Cross-species complementation test of OsRTHs in ''Arabidopsis''('''fig.2''').<br /> <br /> Functional analyses of plant RTHs in Arabidopsis. The seedling triple-response phenotype of ethylene-grown wild type (Col-0) and ''35S:OsRTH1'' transformation lines (A), (''ETR1'')4LOF and a mutant expressing ''35S:OsRTH1'' (B),'' etr1''-7 and a mutant expressing ''35S:OsRTH1'' (C), and ''etr1-2 rte1-2 ''and a mutant expressing ''35S:OsRTH1'' (D). (E) The seedling triple-response phenotype of the wild type (Col-0) expressing ''35S:OsRTH2'' and ''35S:OsRTH3.'' (F) Seedling triple-response phenotype of ''etr1-2 rte1-2 ''expressing ''35S:OsRTH2'' and'' OsRTH3''. Leaf senescence phenotype of the wild type (Col-0) (G) and ethylene-insensitive ''etr1-2'' (H); phenotype of wild type (Col-0), (''ETR1'')4LOF (J), ''etr1-2 rte1-2'' (K), and'' etr1-7'' (L) expressing ''35S:OsRTH1''. Air and ethylene indicate the phenotype of the same plants before and after the treatment, respectively. Chlorophyll a measurement (M) and SAG12 expression (N) of the wild type (Col-0), ''etr1-2'', and ''35S:OsRTH1'' transformants in the corresponding mutation background as indicated. Error bars indicate the standard error (SE) for the means of five measurements. RT-PCR, analysis of the mRNA level of corresponding transgenes at the translational level. a (air) and b (ethylene) indicate a statistically significant difference (a-0.01) between the wild type and mutant or transformation lines.<br /> <br /> <br /> <br /> '''Fig.2 expression in ''Arabidopsis'''''<br /> [[File:Fig.2 expression.png]]<br /> <br /> <br /> <br /> 2. GFP-fused OsRTHs are associated with the Golgi('''fig.3''').<br /> <br /> '''Fig. 3 GFP-fused OsRTHs location'''<br /> <br /> [[File:Fig. 3 GFP.png]]<br /> <br /> <br /> <br /> <br /> 3. OsRTH1 overexpression prevents ethylene-induced riceleaf senescence('''fig.4''').<br /> <br /> '''Fig. 4 OsRTH1 overexpression in rice'''<br /> [[File:Fig. 4 corn.png]]<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> <br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 1A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 1B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 1C).<br /> <br /> <br /> <br /> '''Fig. 1'''<br /> [[File:Fig.1_evolution.png]]<br /> <br /> ==Labs working on this gene==<br /> 1. National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai), Institute of Plant<br /> Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.<br /> <br /> 2. Plant Gene Research Center, National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese<br /> Academy of Sciences.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172074 2014-05-25T03:55:34Z

<p>Damonsong: /* Expression */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> 1. Cross-species complementation test of OsRTHs in ''Arabidopsis''('''fig.2''').<br /> <br /> Functional analyses of plant RTHs in Arabidopsis. The seedling triple-response phenotype of ethylene-grown wild type (Col-0) and ''35S:OsRTH1'' transformation lines (A), (''ETR1'')4LOF and a mutant expressing ''35S:OsRTH1'' (B),'' etr1''-7 and a mutant expressing ''35S:OsRTH1'' (C), and ''etr1-2 rte1-2 ''and a mutant expressing ''35S:OsRTH1'' (D). (E) The seedling triple-response phenotype of the wild type (Col-0) expressing ''35S:OsRTH2'' and ''35S:OsRTH3.'' (F) Seedling triple-response phenotype of ''etr1-2 rte1-2 ''expressing ''35S:OsRTH2'' and'' OsRTH3''. Leaf senescence phenotype of the wild type (Col-0) (G) and ethylene-insensitive ''etr1-2'' (H); phenotype of wild type (Col-0), (''ETR1'')4LOF (J), ''etr1-2 rte1-2'' (K), and'' etr1-7'' (L) expressing ''35S:OsRTH1''. Air and ethylene indicate the phenotype of the same plants before and after the treatment, respectively. Chlorophyll a measurement (M) and SAG12 expression (N) of the wild type (Col-0), ''etr1-2'', and ''35S:OsRTH1'' transformants in the corresponding mutation background as indicated. Error bars indicate the standard error (SE) for the means of five measurements. RT-PCR, analysis of the mRNA level of corresponding transgenes at the translational level. a (air) and b (ethylene) indicate a statistically significant difference (a-0.01) between the wild type and mutant or transformation lines.<br /> <br /> <br /> <br /> '''Fig.2 expression in ''Arabidopsis'''''<br /> [[File:Fig.2 expression.png]]<br /> <br /> <br /> <br /> 2. GFP-fused OsRTHs are associated with the Golgi('''fig.3''').<br /> <br /> '''Fig. 3 GFP-fused OsRTHs location'''<br /> <br /> [[File:Fig. 3 GFP.png]]<br /> <br /> <br /> <br /> <br /> 3. OsRTH1 overexpression prevents ethylene-induced riceleaf senescence('''fig.4''').<br /> <br /> '''Fig. 4 OsRTH1 overexpression in rice'''<br /> [[File:Fig. 4 corn.png]]<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> <br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 1A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 1B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 1C).<br /> <br /> <br /> <br /> '''Fig. 1'''<br /> [[File:Fig.1_evolution.png]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172073 2014-05-25T03:53:44Z

<p>Damonsong: /* Expression */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> 1. Cross-species complementation test of OsRTHs in ''Arabidopsis''('''fig.2''').<br /> <br /> Functional analyses of plant RTHs in Arabidopsis. The seedling triple-response phenotype of ethylene-grown wild type (Col-0) and ''35S:OsRTH1'' transformation lines (A), (''ETR1'')4LOF and a mutant expressing ''35S:OsRTH1'' (B),'' etr1''-7 and a mutant expressing ''35S:OsRTH1'' (C), and ''etr1-2 rte1-2 ''and a mutant expressing ''35S:OsRTH1'' (D). (E) The seedling triple-response phenotype of the wild type (Col-0) expressing ''35S:OsRTH2'' and ''35S:OsRTH3.'' (F) Seedling triple-response phenotype of ''etr1-2 rte1-2 ''expressing ''35S:OsRTH2'' and'' OsRTH3''. Leaf senescence phenotype of the wild type (Col-0) (G) and ethylene-insensitive ''etr1-2'' (H); phenotype of wild type (Col-0), (''ETR1'')4LOF (J), ''etr1-2 rte1-2'' (K), and'' etr1-7'' (L) expressing ''35S:OsRTH1''. Air and ethylene indicate the phenotype of the same plants before and after the treatment, respectively. Chlorophyll a measurement (M) and SAG12 expression (N) of the wild type (Col-0), ''etr1-2'', and ''35S:OsRTH1'' transformants in the corresponding mutation background as indicated. Error bars indicate the standard error (SE) for the means of five measurements. RT-PCR, analysis of the mRNA level of corresponding transgenes at the translational level. a (air) and b (ethylene) indicate a statistically significant difference (a-0.01) between the wild type and mutant or transformation lines.<br /> <br /> <br /> <br /> '''Fig.2 expression'''<br /> [[File:Fig.2 expression.png]]<br /> <br /> <br /> <br /> 2. GFP-fused OsRTHs are associated with the Golgi('''fig.3''').<br /> <br /> '''Fig. 3 GFP'''<br /> <br /> [[File:Fig. 3 GFP.png]]<br /> <br /> <br /> <br /> <br /> 3. OsRTH1 overexpression prevents ethylene-induced riceleaf senescence('''fig.4''').<br /> <br /> '''Fig. 4'''<br /> [[File:Fig. 4 corn.png]]<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> <br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 1A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 1B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 1C).<br /> <br /> <br /> <br /> '''Fig. 1'''<br /> [[File:Fig.1_evolution.png]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Fig._4_corn.png&diff=172072 2014-05-25T03:53:32Z

<p>Damonsong: </p> <hr /> <div></div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172070 2014-05-25T03:51:00Z

<p>Damonsong: /* Expression */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> 1. Cross-species complementation test of OsRTHs in ''Arabidopsis''('''fig.2''').<br /> <br /> Functional analyses of plant RTHs in Arabidopsis. The seedling triple-response phenotype of ethylene-grown wild type (Col-0) and ''35S:OsRTH1'' transformation lines (A), (''ETR1'')4LOF and a mutant expressing ''35S:OsRTH1'' (B),'' etr1''-7 and a mutant expressing ''35S:OsRTH1'' (C), and ''etr1-2 rte1-2 ''and a mutant expressing ''35S:OsRTH1'' (D). (E) The seedling triple-response phenotype of the wild type (Col-0) expressing ''35S:OsRTH2'' and ''35S:OsRTH3.'' (F) Seedling triple-response phenotype of ''etr1-2 rte1-2 ''expressing ''35S:OsRTH2'' and'' OsRTH3''. Leaf senescence phenotype of the wild type (Col-0) (G) and ethylene-insensitive ''etr1-2'' (H); phenotype of wild type (Col-0), (''ETR1'')4LOF (J), ''etr1-2 rte1-2'' (K), and'' etr1-7'' (L) expressing ''35S:OsRTH1''. Air and ethylene indicate the phenotype of the same plants before and after the treatment, respectively. Chlorophyll a measurement (M) and SAG12 expression (N) of the wild type (Col-0), ''etr1-2'', and ''35S:OsRTH1'' transformants in the corresponding mutation background as indicated. Error bars indicate the standard error (SE) for the means of five measurements. RT-PCR, analysis of the mRNA level of corresponding transgenes at the translational level. a (air) and b (ethylene) indicate a statistically significant difference (a-0.01) between the wild type and mutant or transformation lines.<br /> <br /> <br /> <br /> '''Fig.2 expression'''<br /> [[File:Fig.2 expression.png]]<br /> <br /> 2.GFP-fused OsRTHs are associated with the Golgi('''fig.3''')<br /> <br /> '''Fig. 3 GFP'''<br /> <br /> [[File:Fig. 3 GFP.png]]<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> <br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 1A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 1B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 1C).<br /> <br /> <br /> <br /> '''Fig. 1'''<br /> [[File:Fig.1_evolution.png]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Fig._3_GFP.png&diff=172068 2014-05-25T03:49:47Z

<p>Damonsong: </p> <hr /> <div></div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172064 2014-05-25T03:46:45Z

<p>Damonsong: /* Expression */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> 1. Cross-species complementation test of OsRTHs in ''Arabidopsis''('''fig.2''').<br /> <br /> Functional analyses of plant RTHs in Arabidopsis. The seedling triple-response phenotype of ethylene-grown wild type (Col-0) and ''35S:OsRTH1'' transformation lines (A), (''ETR1'')4LOF and a mutant expressing ''35S:OsRTH1'' (B),'' etr1''-7 and a mutant expressing ''35S:OsRTH1'' (C), and ''etr1-2 rte1-2 ''and a mutant expressing ''35S:OsRTH1'' (D). (E) The seedling triple-response phenotype of the wild type (Col-0) expressing ''35S:OsRTH2'' and ''35S:OsRTH3.'' (F) Seedling triple-response phenotype of ''etr1-2 rte1-2 ''expressing ''35S:OsRTH2'' and'' OsRTH3''. Leaf senescence phenotype of the wild type (Col-0) (G) and ethylene-insensitive ''etr1-2'' (H); phenotype of wild type (Col-0), (''ETR1'')4LOF (J), ''etr1-2 rte1-2'' (K), and'' etr1-7'' (L) expressing ''35S:OsRTH1''. Air and ethylene indicate the phenotype of the same plants before and after the treatment, respectively. Chlorophyll a measurement (M) and SAG12 expression (N) of the wild type (Col-0), ''etr1-2'', and ''35S:OsRTH1'' transformants in the corresponding mutation background as indicated. Error bars indicate the standard error (SE) for the means of five measurements. RT-PCR, analysis of the mRNA level of corresponding transgenes at the translational level. a (air) and b (ethylene) indicate a statistically significant difference (a-0.01) between the wild type and mutant or transformation lines.<br /> <br /> <br /> <br /> '''Fig.2 expression'''<br /> [[File:Fig.2 expression.png]]<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> <br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 1A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 1B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 1C).<br /> <br /> <br /> <br /> '''Fig. 1'''<br /> [[File:Fig.1_evolution.png]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172062 2014-05-25T03:46:35Z

<p>Damonsong: /* Expression */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> 1. Cross-species complementation test of OsRTHs in ''Arabidopsis''('''fig.2''').<br /> Functional analyses of plant RTHs in Arabidopsis. The seedling triple-response phenotype of ethylene-grown wild type (Col-0) and ''35S:OsRTH1'' transformation lines (A), (''ETR1'')4LOF and a mutant expressing ''35S:OsRTH1'' (B),'' etr1''-7 and a mutant expressing ''35S:OsRTH1'' (C), and ''etr1-2 rte1-2 ''and a mutant expressing ''35S:OsRTH1'' (D). (E) The seedling triple-response phenotype of the wild type (Col-0) expressing ''35S:OsRTH2'' and ''35S:OsRTH3.'' (F) Seedling triple-response phenotype of ''etr1-2 rte1-2 ''expressing ''35S:OsRTH2'' and'' OsRTH3''. Leaf senescence phenotype of the wild type (Col-0) (G) and ethylene-insensitive ''etr1-2'' (H); phenotype of wild type (Col-0), (''ETR1'')4LOF (J), ''etr1-2 rte1-2'' (K), and'' etr1-7'' (L) expressing ''35S:OsRTH1''. Air and ethylene indicate the phenotype of the same plants before and after the treatment, respectively. Chlorophyll a measurement (M) and SAG12 expression (N) of the wild type (Col-0), ''etr1-2'', and ''35S:OsRTH1'' transformants in the corresponding mutation background as indicated. Error bars indicate the standard error (SE) for the means of five measurements. RT-PCR, analysis of the mRNA level of corresponding transgenes at the translational level. a (air) and b (ethylene) indicate a statistically significant difference (a-0.01) between the wild type and mutant or transformation lines.<br /> <br /> <br /> <br /> '''Fig.2 expression'''<br /> [[File:Fig.2 expression.png]]<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> <br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 1A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 1B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 1C).<br /> <br /> <br /> <br /> '''Fig. 1'''<br /> [[File:Fig.1_evolution.png]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172061 2014-05-25T03:45:38Z

<p>Damonsong: /* Expression */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> 1. Cross-species complementation test of OsRTHs in ''Arabidopsis''.<br /> Functional analyses of plant RTHs in Arabidopsis. The seedling triple-response phenotype of ethylene-grown wild type (Col-0) and ''35S:OsRTH1'' transformation lines (A), (''ETR1'')4LOF and a mutant expressing ''35S:OsRTH1'' (B),'' etr1''-7 and a mutant expressing ''35S:OsRTH1'' (C), and ''etr1-2 rte1-2 ''and a mutant expressing ''35S:OsRTH1'' (D). (E) The seedling triple-response phenotype of the wild type (Col-0) expressing ''35S:OsRTH2'' and ''35S:OsRTH3.'' (F) Seedling triple-response phenotype of ''etr1-2 rte1-2 ''expressing ''35S:OsRTH2'' and'' OsRTH3''. Leaf senescence phenotype of the wild type (Col-0) (G) and ethylene-insensitive ''etr1-2'' (H); phenotype of wild type (Col-0), (''ETR1'')4LOF (J), ''etr1-2 rte1-2'' (K), and'' etr1-7'' (L) expressing ''35S:OsRTH1''. Air and ethylene indicate the phenotype of the same plants before and after the treatment, respectively. Chlorophyll a measurement (M) and SAG12 expression (N) of the wild type (Col-0), ''etr1-2'', and ''35S:OsRTH1'' transformants in the corresponding mutation background as indicated. Error bars indicate the standard error (SE) for the means of five measurements. RT-PCR, analysis of the mRNA level of corresponding transgenes at the translational level. a (air) and b (ethylene) indicate a statistically significant difference (a-0.01) between the wild type and mutant or transformation lines.<br /> <br /> <br /> <br /> '''Fig.2 expression.png'''<br /> [[File:Fig.2 expression.png]]<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> <br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 1A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 1B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 1C).<br /> <br /> <br /> <br /> '''Fig. 1'''<br /> [[File:Fig.1_evolution.png]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172060 2014-05-25T03:45:25Z

<p>Damonsong: /* Expression */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> 1. Cross-species complementation test of OsRTHs in ''Arabidopsis''.<br /> Functional analyses of plant RTHs in Arabidopsis. The seedling triple-response phenotype of ethylene-grown wild type (Col-0) and ''35S:OsRTH1'' transformation lines (A), (''ETR1'')4LOF and a mutant expressing ''35S:OsRTH1'' (B),'' etr1''-7 and a mutant expressing ''35S:OsRTH1'' (C), and ''etr1-2 rte1-2 ''and a mutant expressing ''35S:OsRTH1'' (D). (E) The seedling triple-response phenotype of the wild type (Col-0) expressing ''35S:OsRTH2'' and ''35S:OsRTH3.'' (F) Seedling triple-response phenotype of ''etr1-2 rte1-2 ''expressing ''35S:OsRTH2'' and'' OsRTH3''. Leaf senescence phenotype of the wild type (Col-0) (G) and ethylene-insensitive ''etr1-2'' (H); phenotype of wild type (Col-0), (''ETR1'')4LOF (J), ''etr1-2 rte1-2'' (K), and'' etr1-7'' (L) expressing ''35S:OsRTH1''. Air and ethylene indicate the phenotype of the same plants before and after the treatment, respectively. Chlorophyll a measurement (M) and SAG12 expression (N) of the wild type (Col-0), ''etr1-2'', and ''35S:OsRTH1'' transformants in the corresponding mutation background as indicated. Error bars indicate the standard error (SE) for the means of five measurements. RT-PCR, analysis of the mRNA level of corresponding transgenes at the translational level. a (air) and b (ethylene) indicate a statistically significant difference (a-0.01) between the wild type and mutant or transformation lines.<br /> <br /> <br /> <br /> '''Fig.2 expression.png'''<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> <br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 1A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 1B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 1C).<br /> <br /> <br /> <br /> '''Fig. 1'''<br /> [[File:Fig.1_evolution.png]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172059 2014-05-25T03:45:07Z

<p>Damonsong: /* Expression */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> 1. Cross-species complementation test of OsRTHs in ''Arabidopsis''.<br /> Functional analyses of plant RTHs in Arabidopsis. The seedling triple-response phenotype of ethylene-grown wild type (Col-0) and ''35S:OsRTH1'' transformation lines (A), (''ETR1'')4LOF and a mutant expressing ''35S:OsRTH1'' (B),'' etr1''-7 and a mutant expressing ''35S:OsRTH1'' (C), and ''etr1-2 rte1-2 ''and a mutant expressing ''35S:OsRTH1'' (D). (E) The seedling triple-response phenotype of the wild type (Col-0) expressing ''35S:OsRTH2'' and ''35S:OsRTH3.'' (F) Seedling triple-response phenotype of ''etr1-2 rte1-2 ''expressing ''35S:OsRTH2'' and'' OsRTH3''. Leaf senescence phenotype of the wild type (Col-0) (G) and ethylene-insensitive ''etr1-2'' (H); phenotype of wild type (Col-0), (''ETR1'')4LOF (J), ''etr1-2 rte1-2'' (K), and'' etr1-7'' (L) expressing ''35S:OsRTH1''. Air and ethylene indicate the phenotype of the same plants before and after the treatment, respectively. Chlorophyll a measurement (M) and SAG12 expression (N) of the wild type (Col-0), ''etr1-2'', and ''35S:OsRTH1'' transformants in the corresponding mutation background as indicated. Error bars indicate the standard error (SE) for the means of five measurements. RT-PCR, analysis of the mRNA level of corresponding transgenes at the translational level. a (air) and b (ethylene) indicate a statistically significant difference (a-0.01) between the wild type and mutant or transformation lines.<br /> <br /> <br /> <br /> '''Fig.2 expression.png'''<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> <br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 1A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 1B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 1C).<br /> <br /> <br /> <br /> '''Fig. 1'''<br /> [[File:Fig.1_evolution.png]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172058 2014-05-25T03:44:13Z

<p>Damonsong: /* Expression */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> 1. Cross-species complementation test of OsRTHs in ''Arabidopsis''.<br /> Functional analyses of plant RTHs in Arabidopsis. The seedling triple-response phenotype of ethylene-grown wild type (Col-0) and ''35S:OsRTH1'' transformation lines (A), (''ETR1'')4LOF and a mutant expressing ''35S:OsRTH1'' (B),'' etr1''-7 and a mutant expressing ''35S:OsRTH1'' (C), and ''etr1-2 rte1-2 ''and a mutant expressing ''35S:OsRTH1'' (D). (E) The seedling triple-response phenotype of the wild type (Col-0) expressing ''35S:OsRTH2'' and ''35S:OsRTH3.'' (F) Seedling triple-response phenotype of ''etr1-2 rte1-2 ''expressing ''35S:OsRTH2'' and'' OsRTH3''. Leaf senescence phenotype of the wild type (Col-0) (G) and ethylene-insensitive ''etr1-2'' (H); phenotype of wild type (Col-0), (''ETR1'')4LOF (J), ''etr1-2 rte1-2'' (K), and'' etr1-7'' (L) expressing ''35S:OsRTH1''. Air and ethylene indicate the phenotype of the same plants before and after the treatment, respectively. Chlorophyll a measurement (M) and SAG12 expression (N) of the wild type (Col-0), ''etr1-2'', and ''35S:OsRTH1'' transformants in the corresponding mutation background as indicated. Error bars indicate the standard error (SE) for the means of five measurements. RT-PCR, analysis of the mRNA level of corresponding transgenes at the translational level. a (air) and b (ethylene) indicate a statistically significant difference (a-0.01) between the wild type and mutant or transformation lines.<br /> <br /> <br /> <br /> '''Fig.2 expression.png'''<br /> [[File:Fig.2 expression.png]]<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> <br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 1A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 1B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 1C).<br /> <br /> <br /> <br /> '''Fig. 1'''<br /> [[File:Fig.1_evolution.png]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Fig.2_expression.png&diff=172055 2014-05-25T03:43:39Z

<p>Damonsong: </p> <hr /> <div></div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172049 2014-05-25T03:30:29Z

<p>Damonsong: /* Evolution */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> <br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 1A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 1B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 1C).<br /> <br /> <br /> <br /> '''Fig. 1'''<br /> [[File:Fig.1_evolution.png]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172047 2014-05-25T03:29:56Z

<p>Damonsong: /* Evolution */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> <br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 1A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 1B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 1C).<br /> <br /> <br /> <br /> '''''Fig. 1'''''<br /> [[File:Fig.1_evolution.png]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172045 2014-05-25T03:23:58Z

<p>Damonsong: /* Evolution */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> <br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 1A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 1B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 1C).<br /> <br /> <br /> [[File:Fig.1_evolution.png]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172044 2014-05-25T03:23:46Z

<p>Damonsong: /* Evolution */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> <br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 1A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 1B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 1C).<br /> [[File:Fig.1_evolution.png]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Fig.1_evolution.png&diff=172043 2014-05-25T03:23:14Z

<p>Damonsong: </p> <hr /> <div></div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172041 2014-05-25T03:21:05Z

<p>Damonsong: /* Evolution */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> <br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 1A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 1B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 1C).<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172040 2014-05-25T03:20:41Z

<p>Damonsong: /* Evolution */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 1A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 1B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 1C).<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172039 2014-05-25T03:20:31Z

<p>Damonsong: /* Evolution */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> <br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 1A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 1B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 1C).<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172037 2014-05-25T03:19:57Z

<p>Damonsong: /* Evolution */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> The RTH sequence and gene structure of evolutionarily representative plant species, including ''Physcomitrella patens'', Selaginella moellendorffii,'' Arabidopsis'', and rice were compared (Banks et al., 2011).<br /> In addition to the putative, C-terminal transmembrane domains, these RTHs have two conserved regions (CR1 and CR2) and two non-conserved regions (NCR1 and NCR2).<br /> NCR1 may not have a role in RTE1 function because the NCR1-lacking ND49rte1 isoform is still functional in ethylene signalling (Zhou et al., 2007) (Fig. 1A). The<br /> structures of ''AtRTH'','' OsRTH1'' (Os01g0711600), and ''OsRTH3'' (Os03G0799500) are similar to that of ''RTE1''.They have three exons and two introns. The putative start codon is located at the beginning of exon 2 and the stop codon is located at the end of exon 3. ''PpRTH1'' and ''OsRTH2'' (Os05g0539800) are structurally distinct from ''RTE1'' and have three introns and four exons (Fig. 1B). Because of the lack of genomic sequence information, the gene structures for the moss ''PpRTH2'' and lycophyte ''SmRTH'' could not be compared. On pairwise sequence alignment of RTHs and RTE1 (http://www.ebi.ac.uk/Tools/psa/emboss_needle/), RTE1 had the highest sequence identity with and similarity to OsRTH1, and the lowest with AtRTH, OsRTH3, and RTHs of lower plants (Fig. 1C).<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172019 2014-05-25T02:45:33Z

<p>Damonsong: /* Function */</p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Overexpression of ''Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1)'' results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene ''Ethylene Response1 (ETR1)''. There're three types of rice ''RTE1'' homologues ''(OsRTH genes)'', results from a cross-species complementation test in ''Arabidopsis'' showed that ''OsRTH1'' overexpression complemented the ''rte1-2'' loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ''ETR1''-dependent manner. In contrast, ''OsRTH2'' and ''OsRTH3'' overexpression did not complement ''rte1-2'' or confer ethylene insensitivity. In rice, ''OsRTH1'' overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. ''OsRTH1'' may be an ''RTE1'' orthologue of rice and modulate rice ethylene responses.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172004 2014-05-25T02:31:01Z

<p>Damonsong: </p> <hr /> <div>The rice Os01g0711600 gene expresses protein called '''''OsRTH1''''', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0711600&diff=172002 2014-05-25T02:29:47Z

<p>Damonsong: </p> <hr /> <div>The rice Os01g0711600 gene express '''OsRTH1'''''Italic text'', which functionally related with ethylene insensitivity in rice and other species.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0711600|<br /> Description = Protein of unknown function DUF778 family protein|<br /> Version = NM_001050583.1 GI:115439536 GeneID:4325934|<br /> Length = 2967 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0711600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:31328501..31331467|<br /> CDS = 31329927..31330232,31331004..31331423|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:31328501..31331467<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAPNKISSMDAGAAFDDEDASSSNSLQELWPVGEIDPKRARFPC CIVWTPLPIVSWLAPYIGHAGICREDGTVLDFAGSNLVSMDNFAYGSIARYLQLDRKK CCFPVNLATHVCERSYKHAEAGTAISWDDALQLGMRSFGHKFYNLFTCNCYSFVANCL NRLAYNGSVKWNVLNVAALVWLRGQWVDKMSVVRSFFPFLTVTCVGILMAGWPFLIGM AAFSSLLIGWFVFAVYCMKDLVC&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1427..1732#2504..2923#tgcgcttaccatttgcgccgcccatcgcgtccagctagcgcctggcgcggctgcagatgcagctgctgagctgcaggtgcatgcacctttcccacccccacctccctctctccccttcgtcttcctctatagcccgcgccgcgcctccctgtccccgagtccgaagcccgaatccgaagcgaagtgaccgcaagagctcacccccgagtccccccctcgccggatcgcgcgggccgcctacctcctcccgtcgcaccagctaccgcggtgagttgctcccctctcgatctctgctctgttaggttcggttggttcgtggccgtgctggctgcggcagtgttggggaaggacggggggttttgggggctagtttacgattcgattttccctctgctgtgtttgtgtttttcttttccccagcgaggatgaaactattacatatgactatgtggttatgtggtatcgaagcagatcgaataattgatccggaatgtataggagtagtacttttgttcgaatcgacataggcgtttactagtttgtgcggttgggtacaaacaaattttgtaagaggaagcttaatagtgtacccaactactagcttcaattcatctatagccaatcaaatacatcattcatacaatagttacatactacactattaatacttggtcccacctgtcatacacacacttcgtcttggagtccgtgctacactgccattctttctccttatttttcttcttaaaatatgtttgcagctagcttatagcctgctattgtacataatctaatggctaataaggttctcgccgcggcggtaactctgccccttgcgtgagctgagctcagaagatgatgtcaccaagtgcactaatgactcatgtattcttgatgatctttgttgaagtagtttactatttggggtagtataaagtctaaaactgccatttggtgctaatcagggccgttgtagctgtttcttttttcattttgatgccttgatgctctccttgagaattaagatactacaataattgtactagccaaaaagggattaggagtttgaaagatgatgaactggggaaggactaaagtttgtaatgaatggccgcataggtaatgatttgatctgtggagaatttgtaaaggaagttaacaaagtttgttctattcaagaaattgttttgctctcatctctttttccatattcaacatcttttattagattttattgtgacatgacattttgtttgtgtatttgtcataacagaggtaccaaactggatatatgattgagtcttcatctattactttttgtcctgaagtatgggggcctaaatttttgtcattaatttcagggaatgcgaagatcttgcgatggaagctagtcaaatctgatttcgtttaagtacaagtaacaacaatggcaccaaacaaaatttcctcaatggatgctggagcagcttttgatgacgaagatgctagttccagcaattcactgcaggagttgtggccagttggcgagatagatcctaagagagcaagatttccttgttgcattgtttggactcctcttcctattgtctcgtggcttgctccttacatagggcatgctggaatctgtcgagaggatgggactgtcttggattttgctggttcaaatctggtgagcatggataattttgcatatggttctattgcgagatatctccagcttgacagaaagaaggtatgcatgattcgaatatttttcatttcttcatttggaatggtgtacataattcaacctacttgatagtagtttattcatccaaaatggcaaccctcatagctataggatccatgaaaacccttgttgacttaaaaaaaacacactactagtatgttattttgacatttacttcctgtgtcaatagcttcaaaatgatttgtgctgctgttgcaagaaaacaatctgaacttcctctctacttaagttgcttgaaacaaaattttcatgatcttactgttctttaaagagaacattttccaagctaattcctaaatatagaaatctttaaacagtgggctatttaagtaatgataaaacatattttaaaatttccatgcttgagaaaaaagtgaaagaaatataaaaattatgagttggggacactgaacctggggcttcagccagtgccaggtatcacggactgatcatgacagtacatggctagaacaagggcaattgggcaaaggctgaatattactagcgtttgctcatgaaaatctgttgatcaattccaatatttatttaatgatagcagtgttagtgtgttatttacaaataacttgtcaaaaacgtcaaatatgtgctgaagttgcataagatgtttttgtatcatcttaatccatagataaacttcttgtcaattgtcatttcattaaaaaaaatagtagtgtcatttcaaataggttagttttcttgacactctgtatgttctttttttctcttgactagtgttgctttcctgttaatctcgcgacacatgtatgtgagcggtcctacaagcatgcagaagcaggaacggcgatttcctgggacgacgctctgcaactgggcatgagaagtttcgggcacaagttctataatctgtttacctgcaattgctactcatttgtggcaaactgcttgaatcggcttgcctacaatggctccgtgaagtggaatgtgttgaatgtggctgctcttgtttggttgcgtggccaatgggtggataaaatgtctgttgttcgttcattctttccttttctaactgtgacatgcgttggcattttaatggctggctggcctttcctaatagggatggcagccttctcttctctcttgattggatggtttgtatttgctgtatactgcatgaaggatctagtgtgctgactcttttaaagaacattggcactgaatcctgtgatgaattatgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050583.1 RefSeq:Os01g0711600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Damonsong