http://192.168.164.12:81/ricewiki/api.php?action=feedcontributions&feedformat=atom&user=DaweihRiceWiki - User contributions [en]2026-05-07T07:38:01ZUser contributionsMediaWiki 1.30.0https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Rice_expression_breadth.png&diff=184365File:Rice expression breadth.png2014-07-21T14:54:42Z<p>Daweih: </p>
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<div></div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Seedling_four-leaf_stage.rpkm.jpeg&diff=184364File:Seedling four-leaf stage.rpkm.jpeg2014-07-21T14:38:41Z<p>Daweih: </p>
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<div></div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Shoots.rpkm.jpeg&diff=184363File:Shoots.rpkm.jpeg2014-07-21T14:38:27Z<p>Daweih: </p>
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<div></div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Endosperm-25_DAP.rpkm.jpeg&diff=184362File:Endosperm-25 DAP.rpkm.jpeg2014-07-21T14:38:13Z<p>Daweih: </p>
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<div></div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Seed-5_or_10_DAP.rpkm.jpeg&diff=184361File:Seed-5 or 10 DAP.rpkm.jpeg2014-07-21T14:37:52Z<p>Daweih: </p>
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<div></div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Pistil.rpkm.jpeg&diff=184360File:Pistil.rpkm.jpeg2014-07-21T14:37:26Z<p>Daweih: </p>
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<div></div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Post_or_Pre-emergence_inflorescence.rpkm.jpeg&diff=184359File:Post or Pre-emergence inflorescence.rpkm.jpeg2014-07-21T14:36:33Z<p>Daweih: </p>
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<div></div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Leaves-20_days.rpkm.jpeg&diff=184358File:Leaves-20 days.rpkm.jpeg2014-07-21T14:35:56Z<p>Daweih: </p>
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<div></div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Embryo-25_DAP.rpkm.jpeg&diff=184330File:Embryo-25 DAP.rpkm.jpeg2014-07-21T14:01:04Z<p>Daweih: </p>
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<div></div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Anther.rpkm.jpg&diff=184329File:Anther.rpkm.jpg2014-07-21T13:57:50Z<p>Daweih: </p>
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<div></div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os04g0106300&diff=181809Os04g01063002014-06-09T05:26:40Z<p>Daweih: Created page with "==Structured Information== {{JaponicaGene| GeneName = Os04g0106300| Description = Similar to Arginase (EC 3.5.3.1)| Version = NM_001058548.1 GI:115456825 GeneID:4334912| Lengt..."</p>
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<div>==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os04g0106300|<br />
Description = Similar to Arginase (EC 3.5.3.1)|<br />
Version = NM_001058548.1 GI:115456825 GeneID:4334912|<br />
Length = 4240 bp|<br />
Definition = Oryza sativa Japonica Group Os04g0106300, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|<br />
AP = Chromosome 4:387137..391376|<br />
CDS = 387263..387409,388810..388969,389058..389191,389410..389619,389932..390042<br>,390883..391143|<br />
GCID = <gbrowseImage1><br />
name=NC_008397:387137..391376<br />
source=RiceChromosome04<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008397:387137..391376<br />
source=RiceChromosome04<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgggcggcgtggcggcgggcaccaggtggatccaccacgtccggcggctcagcgccgccaaggtgtcggcggacgccctggagcgcggccagagccgggtcatcgacgcctccctcaccctcatccgcgagcgcgccaagctcaaggcagagttgctgcgcgctcttggtggtgtgaaagcttcagcatgcctcttaggtgttcctcttggtcacaactcatcgttcttacagggacctgcatttgctcctccccggataagggaagccatttggtgtggaagtaccaactctagcacagaagaaggcaaagaactcaatgatcctcgagtgctaacagatgttggtgatgtccccatacaagagattcgtgactgtggtgttgaagatgacagattgatgaatgttgtaagcgagtctgtcaaaacagtgatggaggaagatcctcttcggccattggtcctgggaggcgatcactcaatatcttatccagttgttagggctgtgtctgaaaagcttggtggacctgttgacattcttcaccttgacgcacatccagatatctacgatgcttttgaaggaaacatctattcgcatgcttcttcatttgcaagaataatggaaggaggttatgctaggaggcttctacaggttggaatcagatcaattaccaaagaagggcgtgagcaggggaagagatttggtgtggaacagtatgagatgcgcactttttcaaaagatagggagaagcttgaaagtctgaaacttggggaaggtgtgaagggagtgtacatctcagttgacgtggactgcctcgatcccgctttcgcgccaggtgtctctcacattgagccaggaggcctctccttccgcgacgtgctcaacatcctccataacctgcaaggagatgttgtcgccggagatgtggtggagttcaacccgcagcgtgacacggtggacgggatgacggctatggttgcagccaagctggtccgggagctcacagccaagatctccaagtga</cdnaseq>|<br />
AA = <aaseq>MGGVAAGTRWIHHVRRLSAAKVSADALERGQSRVIDASLTLIRE RAKLKAELLRALGGVKASACLLGVPLGHNSSFLQGPAFAPPRIREAIWCGSTNSSTEE GKELNDPRVLTDVGDVPIQEIRDCGVEDDRLMNVVSESVKTVMEEDPLRPLVLGGDHS ISYPVVRAVSEKLGGPVDILHLDAHPDIYDAFEGNIYSHASSFARIMEGGYARRLLQV GIRSITKEGREQGKRFGVEQYEMRTFSKDREKLESLKLGEGVKGVYISVDVDCLDPAF APGVSHIEPGGLSFRDVLNILHNLQGDVVAGDVVEFNPQRDTVDGMTAMVAAKLVREL TAKISK</aaseq>|<br />
DNA = <dnaseqindica>127..273#1674..1833#1922..2055#2274..2483#2796..2906#3747..4007#ggggcggtgcactgcattattgttgcctcgctcgctcgatcgatcccctctcctctccaaatcccatccccaaatcccgaatcctccatcgagatcgatcgacgtcgagcggagcgaaggggggatatgggcggcgtggcggcgggcaccaggtggatccaccacgtccggcggctcagcgccgccaaggtgtcggcggacgccctggagcgcggccagagccgggtcatcgacgcctccctcaccctcatccgcgagcgcgccaagctcaaggtcctctctctctctctctccccacccatttcatcttcatcctgctttgcagcgatcctctttctccaggttccccttctttttttaaaaagctgcatattcctttcggttcgatttcaccccctttttcttttatttatttatattcgcccagttaatgggtaatcatgtttagtgtcagtatcacccggtctttttcttttcttttcttttccttttactagtggtagcatgtgctctccctttgttaacattgtatcctgctactgctaatcgattagttaattatacaccattctgttgttctcacacttgctctctctctctctctctctctctctctctgcacctgtgcacccaaaaatttttttttttttgctacttaactttactgacttactaccagtaataataatctcttaatgcaacaaccataatatagcacttgcgttcagatagggggcatccttacaacactactctgatgtcgcatttgattctgaaacccccacccacctgtactacccaaaccccccttttttttttacttatgttgcttttaagtcgattcaacacgcaccaaatgttgtaggaccagactttcaacagcacaagtactccacacaagaaattaagtggcttacctttacaatcagctcttccctcaaattgtaggacaagatacaggactatcattaatacgacccttggctttgtcatgaactgactgttgtatgacctgttttttcttcttttctgtgaaatttaatcaatcattaattaaccatctaccaggctttcaaatcatatcgcgaatgcgtaatggatacatgggttggatctgtacacctacattttacatctgctacctcaagtgtttttttgtttgcaactcattttatatatgctacctcaagtgttattttttttgtctgcaacttatgtaacgattgagtccttttaaggctcatatcaaaattatttagtgggccaaggactaagatatggtcaaagatggtccttcgcacacagcattgctttcagcctttgcttcagctgtaatccaacgtcctcttagcttaatctacagatttatgttctttctctgaagcaattatgtttaccatgttgcacctgaatacctaacaaattagagatggaaaagtttaacatttggagctactttgtttgagttcgtaaataaacctatggctcattgagagtggcattaatcgtacattctctgcaaactagtcttagtgtttttttttcagattctaaagattttaaacttcagtttctacaagattacttcattctaggatcaaatcttttaattaatcttgtaacatttctgacaactctgccaaccctgacttggaacttttgcttgtgtgcaggcagagttgctgcgcgctcttggtggtgtgaaagcttcagcatgcctcttaggtgttcctcttggtcacaactcatcgttcttacagggacctgcatttgctcctccccggataagggaagccatttggtgtggaagtaccaactctagcacagaagaaggtacagttaacgtgttcaaactatatagtagcatatcttgtatacaataagcatttattgaaatatgccttgccttcttgttgttcaggcaaagaactcaatgatcctcgagtgctaacagatgttggtgatgtccccatacaagagattcgtgactgtggtgttgaagatgacagattgatgaatgttgtaagcgagtctgtcaaaacagtgatggaggaagtgagcacattatcccaccatgcttgtttcaaaaacttgtttgtcgatcatctcagctatttccttgacagtagttagcataattttttgaagtttatttaaggatctggtgaagtctgacatacatcttaattaattttaggacaaatatttgcttggacaaaacttaaagctattgaagcagagtaatttattaactatgccatacttttatgtaggatcctcttcggccattggtcctgggaggcgatcactcaatatcttatccagttgttagggctgtgtctgaaaagcttggtggacctgttgacattcttcaccttgacgcacatccagatatctacgatgcttttgaaggaaacatctattcgcatgcttcttcatttgcaagaataatggaaggaggttatgctaggaggcttctacaggtacttttatacagcttttcgttgttttttgagggaatcaccaggagggttggtatcccacttgtatatctttgagttctgcattccagaactatcatagtactactgaatggatgtttatctaaacaaagggactactattgaatgaacgaacgaatgaatgaagtatctcttttatttttaagcccaaatttaaggagaaggaaaatacaaacacaatattgaaatattgggttataatcttctctataactagcgcagctgtcattgctctgaaaatatggtttcacctttaatcctgatggttttcaggttggaatcagatcaattaccaaagaagggcgtgagcaggggaagagatttggtgtggaacagtatgagatgcgcactttttcaaaagatagggagaagcttgaaagtctggtaattttactaaaaataaccacgtcaatgtctttgcaatcacctgggtaattttaatatgatgattggggacatatagtattgatgtgtgttatgattctttgtagtgtgataattgcaaatttgcacttggccattcatttaaacatactaatgtatctatttggcccaaaggagttttagggtccttttgggctctgttactgcctccacagcttttggtctctcttcaagttgcaactctgatacctctaccataatgttagataaactatcatgggattaagtttaatgctgtaatctattcgtagatgctctgtttggacagccttgtctacaacaggttggcaaccaaggccatgggtgtctagtttgaactttcaatgctttggcgatgttaacctaggaaactttggtagcttggaccaagatttgccattaacttggtttgtcaccaaaatttggcaaggggccgttcgcattggctccctaatctgtttttataaccaaacttcatcagtcgatgggctttgtagtgtgccttgtggaaatagctattcataaaattgcttaaaaaataaagtggcagagtgacagttaccttgtcaaaaccatcaagatctgttggaaagtcaccctgttacctgtcattgttgtatttaaccagctgaaaaaggaaggttcacaagtggaacttaggattaagccaatgttttatttattttccaaagtaaaattttgatgccatccaatctatgtatatgtgtttcccagttgggatccatagcatggatatccttttatccaaatgtctaacttattctttttatttattggcagaaacttggggaaggtgtgaagggagtgtacatctcagttgacgtggactgcctcgatcccgctttcgcgccaggtgtctctcacattgagccaggaggcctctccttccgcgacgtgctcaacatcctccataacctgcaaggagatgttgtcgccggagatgtggtggagttcaacccgcagcgtgacacggtggacgggatgacggctatggttgcagccaagctggtccgggagctcacagccaagatctccaagtgagcatccattcagattcagggcatatcatatcaccaaccaaccccttgagtctgaagcagcaaagaggatgattcccagactcctttagctgttagtctaggttcctatgtagtagacatcagctatgccagattttgtatgtgaatatactcattaggttgcaataatgtttgcctccattttgcacttgtgatgttatggttatccctcatcatcgtgtgctagaagaatgc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001058548.1 RefSeq:Os04g0106300]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 4]]<br />
[[Category:Chromosome 4]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os04g0473900&diff=172941Os04g04739002014-05-26T11:18:49Z<p>Daweih: /* Structured Information */</p>
<hr />
<div>''DWA1'' encodes an enzymatic megaprotein conserved in vascular plants，and ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice.<br />
<br />
==Annotated Information==<br />
===Function===<br />
''DWA1'' encodes an enzymatic megaprotein (2,391 aa in length) conserved in vascular plants, including an oxidoreductase-like domain, a prokaryotic nonribosomal peptide synthetase-like module, an AMP-binding domain, and an allene oxide synthase-like domain. The AMP-binding domain exhibits in vitro enzymatic activity in activating long-chain fatty acids to form acyl-CoA. Expression pattern analysis shows that ''DWA1'' is preferentially expressed in vascular tissues and epidermal layers and strongly induced by drought stress. The ''dwa1'' mutant was highly sensitive to drought stress compared to the WT and DWA1-functional complementation （DWA1-FC）plants restored drought resistance. Further dissections uncovers that the ''dwa1'' mutant was impaired in cuticular wax accumulation and significantly suppressed many wax-related genes expression under drought conditions and reduced levels of very-long-chain fatty acids. Conversely, plants overexpressing ''DWA1'' were significantly up-regulated many wax-related genes expression under drought conditions and elevated levels of very-long-chain fatty acids. The results suggest that ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice<ref name="ref1"/>.<br />
<br />
===Mutation===<br />
[[File:图片3.png|right|thumb|200px|Fig.1 Identification of the drought-hypersensitive ''dwa1'' mutant in rice, in which functional complementation of ''dwa1'' restore the drought resistance(from reference<ref name="ref1" />)]]<br />
'''(1)'''There is an insertion of T-DNA in the sixth exon of ''DWA1'' (Fig.1A).<br><br />
'''(2)'''RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1B).<br><br />
'''(3)'''During the course of drought treatment, the mutant wilted and exhibited leaf rolling earlier than the WT.After severe drought treatment followed by rewatering, more than 90% of the WT plants survive, whereas the ''dwa1'' mutant plants are almost dead(Fig.1C and D).<br><br />
'''(4)'''Water loss rate of mutant is faster than WT(Fig.1E).<br><br />
'''(5)'''DWA1-functional complementation(DWA1-FC)lines which is inserted the full-length genomic fragment of ''DWA1'' into the ''dwa1'' mutant restore drought resistance(Fig.1F and G).<br><br />
'''(6)'''Plant height at the reproductive stage are also restored in the DWA1-FC lines(Fig.1H).<br />
<br />
===Expression===<br />
[[File:图片4.png|right|thumb|200px|Fig.2 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference<ref name="ref1" />)]]<br />
[[File:图片5.png|right|thumb|200px|Fig.3 Drought-induced expression of ''DWA1''.(from reference<ref name="ref1" />)]]<br />
'''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.2A).<br><br />
'''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.2B 1-2), axillary bud (Fig.2B3), young panicle (Fig.2B4),spikelet (Fig.2B5), stem (Fig.2B6), young panicle (Fig.2B7), pistil (Fig.2B8), stamen (Fig.2B9), young embryo (Fig.2B10), SEM picture of rice leaf cuticle (Fig.2B11), cross-sections of stem (Fig.2B12) and leaf (Fig.2B13),and semithin sections of leaf (Fig.2B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.<br><br />
'''(3)'''What's more,after various phytohormones treatment,''DWA1'' expresses higher,such as abscisic acid, jasmonic acid, indole-3-acetic acid, and<br />
gibberellic acid (Fig.2C).<br><br />
'''(4)'''Under drought treatment,GUS staining shows that''DWA1'' is induced.For example,in Fig.3B,GUS staining in leaf before (1 and 3) and after (2 and 4) drought stress is shown.<br><br />
<br />
===Evolution===<br />
[[File:1(DWA1).png|right|thumb|200px|Fig.4 Unrooted phylogenetic tree and domain architecture of DWA1 protein orthologs in vascular plants and microbial relatives(from reference<ref name="ref1" />)]]<br />
At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region<ref name="ref1"/>(Fig.4).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi<ref name="ref2"/>,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain<ref name="ref1"/>.<br />
<br />
==Labs working on this gene==<br />
*National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan,China<br />
<br />
==References==<br />
<references><br />
<ref name="ref1">Xiaoyi Zhu, Lizhong Xiong.（2013）Putative megaenzyme DWA1 plays essential roles in drought resistance by regulating stress-induced wax<br />
deposition in rice.PANS 110(44): 17790-17795.</ref><br />
<ref name="ref2">Marahiel MA, Stachelhaus T, Mootz HD.(1997) Modular peptide synthetases involved in nonribosomal peptide synthesis.Chem Rev 97(7):2651–2674.</ref><br />
</references></div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os04g0473900&diff=171566Os04g04739002014-05-24T04:23:54Z<p>Daweih: </p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
<br />
==Annotated Information==<br />
===Function===<br />
This gene is regarded as ''DWA1''.Putative ''DWA1'' plays important roles in drought resistance.The ''DWA1'' is composed of seven exons(Fig.1) The open reading fame (7,176 bp) encodes a protein consisting of 2,391 aa in ''japonica'' rice, Nipponbare, which is one of the 30 megaproteins (>2,300 aa) predicted in the rice genome<ref name="ref1"/>.<br />
<br />
===Mutation===<br />
[[File:图片3.png|right|thumb|200px|Fig.1 Identification of the drought-hypersensitive ''dwa1'' mutant in rice, in which functional complementation of ''dwa1'' restore the drought resistance(from reference<ref name="ref1" />)]]<br />
'''(1)'''There is an insertion of T-DNA in the sixth exon of ''DWA1'' (Fig.1A).<br><br />
'''(2)'''RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1B).<br><br />
'''(3)'''During the course of drought treatment, the mutant wilted and exhibited leaf rolling earlier than the WT.After severe drought treatment followed by rewatering, more than 90% of the WT plants survive, whereas the ''dwa1'' mutant plants are almost dead(Fig.1C and D).<br><br />
'''(4)'''Water loss rate of mutant is faster than WT(Fig.1E).<br><br />
'''(5)'''DWA1-functional complementation(DWA1-FC)lines which is inserted the full-length genomic fragment of ''DWA1'' into the ''dwa1'' mutant restore drought resistance(Fig.1F and G).<br><br />
'''(6)'''Plant height at the reproductive stage are also restored in the DWA1-FC lines(Fig.1H).<br />
<br />
===Expression===<br />
[[File:图片4.png|right|thumb|200px|Fig.2 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference<ref name="ref1" />)]]<br />
[[File:图片5.png|right|thumb|200px|Fig.3 Drought-induced expression of DWA1.(from reference<ref name="ref1" />)]]<br />
'''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.2A).<br><br />
'''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.2B 1-2), axillary bud (Fig.2B3), young panicle (Fig.2B4),spikelet (Fig.2B5), stem (Fig.2B6), young panicle (Fig.2B7), pistil (Fig.2B8), stamen (Fig.2B9), young embryo (Fig.2B10), SEM picture of rice leaf cuticle (Fig.2B11), cross-sections of stem (Fig.2B12) and leaf (Fig.2B13),and semithin sections of leaf (Fig.2B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.<br><br />
'''(3)'''What's more,after various phytohormones treatment,''DWA1'' expresses higher,such as abscisic acid, jasmonic acid, indole-3-acetic acid, and<br />
gibberellic acid (Fig.2C).<br><br />
'''(4)'''Under drought treatment,GUS staining shows that''DWA1'' is induced.For example,in Fig.3B,GUS staining in leaf before (1 and 3) and after (2 and 4) drought stress is shown.<br />
<br />
===Evolution===<br />
[[File:1(DWA1).png|right|thumb|200px|Fig.2 Unrooted phylogenetic tree and domain architecture of DWA1 protein orthologs in vascular plants and microbial relatives(from reference<ref name="ref1" />)]]<br />
At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region<ref name="ref1"/>(Fig.2).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi<ref name="ref2"/>,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain<ref name="ref1"/>.<br />
<br />
==Labs working on this gene==<br />
*National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan,China<br />
<br />
<br />
==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os04g0473900|<br />
Description = Adrenodoxin reductase family protein|<br />
Version = NM_001187049.1 GI:297723228 GeneID:9266980|<br />
Length = 9882 bp|<br />
Definition = Oryza sativa Japonica Group Os04g0473900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|<br />
AP = Chromosome 4:24097480..24107361|<br />
CDS = 24097480..24100068,24100784..24104053,24104151..24104329,24104410..24104769,24104855..24105296<br>,24105409..24105542,24105689..24105892,24107127..24107361|<br />
GCID = <gbrowseImage1><br />
name=NC_008397:24097480..24107361<br />
source=RiceChromosome04<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008397:24097480..24107361<br />
source=RiceChromosome04<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactgatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttctggctcagaaaggcatctcaattccagattcttctgatcaggttggacttgttgtgattgcagaggttagagagggtaaggcagtctctaaagaggttgttaacaacatcaaagcacgtgtggtggaggagcatggtgtagctgttgcatctgtgaagcttatcaagcctagaaccatatgcaagacaacatctgggaagatacgaagatttgaatgcatgaggcagtttgttgacaatactcttagtttggctaagggcaaccatgtatctaagaaaaagggtctttttcggtctctgacaacaggaactggcatggagtcaaagaggtccctcttgaggcaaactgttgacctcaccattagccattggcccaaaagccaagttaaaaactccaatgaaataactgagtttctgacacaaatagtgtcagagcacactggcatttcaaaggataaaatttctctaactgacagtctaccttcgtatggttttgattctattgcagtggtacgagcagcccagaagctctcagattttcttggtgttcctgttggggccattgacatctttacagcaagctgcatttcggagcttgcaagcttcttggagaatttggtgcataagtcacaaacacagttggcaccttggcccaaaagcaaagtgaaaaactccaaggtgattatcgagtttctgacaaagattgtatcagatcaaacagggattccaaaggataaaatttctccgatggatagcctaccttcatatggttttgattcaattgcggtggtccaagcagcacagaaactctccgattttctcggtgtacctgttggagccattgacatatttacagctggttgcatttctgagctagctaccttcttggagaatttggcgcataagtcacagtcacagttggcacctggtgcatcttgctatattgaggatgagactcaagtggatcccatggatgccattagtccagaattcagtgttttgggtacgggcatcctgcaactgctggctcttacttatgtctgttttgtgctgctactcccagcatatcttgctagttcgacatacatgagcattttctctacagtcagtctggtcagatcaccattgctgagctatcttagttctttggttatggcacctattgtgtggatcttttatatctcgctcacttctttatcactgtctatcttggggaagtccttcctacagccaaattatgttctgatccctgatgtttcaatatggtcagttgactttgtgaaatggtgggcactaaacaaagcacaagcattagctgcaaagatgctagctgtgcatctaaagggcacaattttcttaaattattggttcaagatgcaaggagctcggattggttcatctgttgtcatcgacacagtagacataaccgatccatccctgttgacggttgcagatggtgcagttcttgcagaaggcgctcttgttcagggccatgaagtctgtaatgaagtgctgagttttaggcctatctggattggctgtgaggcttcgattggtccttatgcggtgcttcagaagggaactgttgtggaagatggagctgttgttccaccattgcagaagactggggctgggaaatcaacacgtcggactagcagaacttctgtaagcataaagaaagaagcagccaaagcaaacatgatacttgaacacctcgttagcatctacgcagttggcatcctgggtgctctgtccggtgcaatcgtctacacactatacactcacttgtccggcaaagcggcttcgcctctgcatttctcctttgcatgcattgctggtgcgttccactggttaccagctgccatcactgcctatgctgttattgtccaggagacacccacaagtgcactgtcatttgctctcttcactgccttcgccgacctgagctatggtgtcatcctcagtattctcaccagtataaccagcagagcccttgctgcaaagcctggaaccaagcagaatggcattgcgagtttgatccaccggcgcataaccatctctgctcatgtcaggtttgccaagatgctatctggaacagaagccttctgcgtgtacctgaggcttctcggagcaaagattggcaggcattgctctatccgagccatcaatccggtggcgaatcctgagctgatcagcgtcggtgatggcgtccatctaggtgacttctgcaatattgttccagggttctactccaaggggggattcaccagtgcagagattaaggtccaggagaacaccgtggttggcagtggcagcttgctcctccctggctgtgtcctccaggagaatgtcatcctcggcgcgctctccgtcgcgccggagaacgctgttctccggcgtggcggcgtctacgtcgggtcacagtctccggccatggtgaagaacaccttgcttgacgaggatgaacggatcgaagagatggatcaggcatacaagaagattgttggcaacctcgcggcgaacttggccatcaccaccatgaacgtcaagtcgaggtacttccaccgcatcggcgtgagcggccgcggcgtcctgaggatgtacgaggagataccgtcgtttccccggcacaagatcttcgcctccggcaagtccttcccggtgatcgtccgccacagcaacagcctgagcgccgacgacgacgcgaggctcgacgcccgcggcgccgcggtgcggatcctctcggacaacgacggcgaggcgcctctcctcgacctcacactcaagagcggcaaggcgttctacgcgcgcaccatcgccgacttcgccacctggctggtctgcggcctgccggcgagggaggagcaggtcaagcgctcgccgcacatccgcgacgccgtctggggctctctccggagcaccgactcgtacaccgtgctccactactactccaacatatgccggctgctgcggttcgacgacggcagggagatgtacgccaagttcaagctccgtcctgctgatccagacgtccccgaggactccggcaaggtggtgccgaggggcatcctgccgccggagaccggcgcgatccccagggacgaggatgacacgcgccctctgctcttcctcgccgacgacttccgccggagagtgggctcaccggacggggtgcgctacgtgttccagctgcagctcagggaggtgcccaccgacgccgccgcccgcgacgtcgccctcgactgcacccggccgtgggacgaggccgagttcccttacatcgacgtcggcgaggtcagcatcggccgcaacctcccgacggaggagacggagaagctcgagttcaacccgttcctccggtgccccgaggtggacgtcatcccggcgacgtcgtgcgcgcagagcgcgtccatcgaccacggccgctcgctggtctacgagatatgccagcgcctgcggaacggcgagccgctgcccgcgtcgtggcgagctttcctggagcagtcggacaccaagatcgacctgtcaggatgcccggtcgctgccgccgcgacacccacaaggtctaacgccggcgacgccacgaaggtgacgctggccaggacgtggtaccaggcgctctgggccacgctgtgccagccgctgctgcaaacgctggtgccgtactctgtcctgggcctcgtcatcttcctcccgctcaggggcctcctcgccgtcgccgcggccacccgcttcccgctctactggctcctcccggcgttctgggccgcgtcgggcgtcgccgccatggccacgtgcgcggcggcgaagtgggcgctggtcggcagcagggtcgacggcgacacggtgcacatctggtcgccggccgtgttcctggacacggtctggcaggccgtgcgcgccgccacggcggagtacttcgccgagctgacgcccgggtcggcgccgttcgcggcgtggatgcgggtgatgggcgcctccgtgtcccccggcgacggcgtgtacgtggactccatgggcgcgctgctgaacccggagatggtgcgcctggagcgcggcgccgccgtcgggcgggacgcgctgctgttcggccacgtgtacgagggcgaggccgggaaggtgaagttcggcgccgtgtccgtcggggaggacgggttcgtcggcagccgcgccgtggccatgcccagcgtcacggtggacgacggcggctgcctcgccgcgctcgggctggccatgaagggggagacggtgaagcacagcatgtaa</cdnaseq>|<br />
AA = <aaseq>MEERRATSPSRHSFCRDALGRRGGESGGGLVYIYRSPLRSPLQR HGLNREWRLERWRWRWRWEAATWRQRAAAHARIGEMDPKRSIDDQFSKLHPSLPVDTR IGIVGAGPSGLSAAYALAKLGYRNVTLFEKCHTVSGMCESIDIEEELGSDFEEMDTHK LSLIDSQTGNIRDLEVAEDYVSMVSLTLKLQDEANKSGRAGLHALSGLASDPTHEFLK QNGINSMPKSVAYGYTASGYGFVQDMPYAFIQEFTRTSMAGKIRRFKHGYMSMWERLS KSLPFEVFCDTQVLNVKRNSCGANVTIKNNNGEKQVLEFDKIILSGAVAFKNSKTYRS SSLTDGESEVVELNNLERELFSKVQTIDYYTTVVKINGFEHIPKGFYYFGEFMEDPTT IGHPVAMQRFFADTNIFLFWSYGNSADIKGSFVAKCVTDVVSSMGGNVQKVILQRRFK YFPHVSSKDMKDGFYERLESQLQGFQNTYYVGGLLAFELTERNASYSIMAVCKHFAID GEGELTPYVKRLFPLSHNRNPSPPRDLGELEGVEFPDLPSLDGYLQYWGTHKVTEKNV IYTWINEEGKLMNRRTYQELHGNASYIAQKLLTSTKPVIKPGDRVLLIHLPGLEFIDA FFGCIRAGVIPVPVLPPDPMQSGGQALLKVENISKMCNAVAILSTSSYHAAVRAGYIK NIVTLAKRVQKCSAQWPDIPWIHTDSWIKNYRRSSDSFNSDTVLFTKPQPSDLCFLQF TSGSTGDAKGVMITHEGLIHNVKTMKKRYRSTSKTVLVSWLPQYHDMGLIGGLFTALV SGGTSVLFSPMIFIRNPLLWLQTINDYHGTHSAGPNFAFELVIRRLEAEKNKVYDLSS MVFLMIAAEPVRQKTVRRFIELTQPFGLSEGVLAPGYGLAENCVYVTCAFGECKPVFI DWQGRVCCGYVEQDDTDTLIRIVDPDSLTEHQEDGVEGEIWISSPSSGVGYWGNSEMS QRTFFNQLKNHPNKKFTRTGDLGRTIDGNLFITGRIKDLIIVAGRNIYSADVEKTVES SSEVLRPGCCAVVGIPEEVLAQKGISIPDSSDQVGLVVIAEVREGKAVSKEVVNNIKA RVVEEHGVAVASVKLIKPRTICKTTSGKIRRFECMRQFVDNTLSLAKGNHVSKKKGLF RSLTTGTGMESKRSLLRQTVDLTISHWPKSQVKNSNEITEFLTQIVSEHTGISKDKIS LTDSLPSYGFDSIAVVRAAQKLSDFLGVPVGAIDIFTASCISELASFLENLVHKSQTQ LAPWPKSKVKNSKVIIEFLTKIVSDQTGIPKDKISPMDSLPSYGFDSIAVVQAAQKLS DFLGVPVGAIDIFTAGCISELATFLENLAHKSQSQLAPGASCYIEDETQVDPMDAISP EFSVLGTGILQLLALTYVCFVLLLPAYLASSTYMSIFSTVSLVRSPLLSYLSSLVMAP IVWIFYISLTSLSLSILGKSFLQPNYVLIPDVSIWSVDFVKWWALNKAQALAAKMLAV HLKGTIFLNYWFKMQGARIGSSVVIDTVDITDPSLLTVADGAVLAEGALVQGHEVCNE VLSFRPIWIGCEASIGPYAVLQKGTVVEDGAVVPPLQKTGAGKSTRRTSRTSVSIKKE AAKANMILEHLVSIYAVGILGALSGAIVYTLYTHLSGKAASPLHFSFACIAGAFHWLP AAITAYAVIVQETPTSALSFALFTAFADLSYGVILSILTSITSRALAAKPGTKQNGIA SLIHRRITISAHVRFAKMLSGTEAFCVYLRLLGAKIGRHCSIRAINPVANPELISVGD GVHLGDFCNIVPGFYSKGGFTSAEIKVQENTVVGSGSLLLPGCVLQENVILGALSVAP ENAVLRRGGVYVGSQSPAMVKNTLLDEDERIEEMDQAYKKIVGNLAANLAITTMNVKS RYFHRIGVSGRGVLRMYEEIPSFPRHKIFASGKSFPVIVRHSNSLSADDDARLDARGA AVRILSDNDGEAPLLDLTLKSGKAFYARTIADFATWLVCGLPAREEQVKRSPHIRDAV WGSLRSTDSYTVLHYYSNICRLLRFDDGREMYAKFKLRPADPDVPEDSGKVVPRGILP PETGAIPRDEDDTRPLLFLADDFRRRVGSPDGVRYVFQLQLREVPTDAAARDVALDCT RPWDEAEFPYIDVGEVSIGRNLPTEETEKLEFNPFLRCPEVDVIPATSCAQSASIDHG RSLVYEICQRLRNGEPLPASWRAFLEQSDTKIDLSGCPVAAAATPTRSNAGDATKVTL ARTWYQALWATLCQPLLQTLVPYSVLGLVIFLPLRGLLAVAAATRFPLYWLLPAFWAA SGVAAMATCAAAKWALVGSRVDGDTVHIWSPAVFLDTVWQAVRAATAEYFAELTPGSA PFAAWMRVMGASVSPGDGVYVDSMGALLNPEMVRLERGAAVGRDALLFGHVYEGEAGK VKFGAVSVGEDGFVGSRAVAMPSVTVDDGGCLAALGLAMKGETVKHSM</aaseq>|<br />
DNA = <dnaseqindica>7294..9882#3309..6578#3033..3211#2593..2952#2066..2507#1820..1953#1470..1673#1..235#atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggaggtatggattccccttccccactcgctgcgtgatgaaccgatgaaccgtagtacttgatgatatgcctgatttgcttcggcattgtgccgcttctctgttagctgggagtttgtggcgtgtcgtttaactgaagtagagatgtgatgatgatctgaggtgagcgccattttttttttttggctaactctgtttgttctattcagtgaatatgagctctcttttttgtcccctttttatcattctgcttctatgggtcgcggagcgttcttggttggtgcagtgcaactgcaatgtcagtatgcatgccatagtaatgtgatggtgggatgtaatgctcctctgcttgctcgagctctttcttggttgtctatctatcttggatgatttgatcaatgtactacttattatctgctgatagtacccggggtccatttgcgctagtattgaacttcttcttctcttataatttgctgaaatcgcgagattatcgcttaggtttatcaatcagaacattatcatgtttatgcttcggttcgttcggtactggcacatgtcaaataagcggaaattctgctactttttcggcttaccatgaatggcacccaacatagcgtggttgttttgtcatgttgaactagtacaaatttttgcaccttaatatttgctttccgtgcatatccaattaacctgcccatcaaacttcttttcagatagctattattggggaaaaatattttcccagcttcttttatagtgactagactgtttatacatgcctgcgtaattgcttccttttgatgagctagtacattatagaccttgattttgttttgatggtacgggttggttattggtgtccatataatgaaattgcaacttgcgaccactccatgatagttacctggtcattccttgttggttaataagctattatttcaaatatgaatgttcatcctacttgtcatattatagacatttgtcacaattcaagtgaggaagaaattctgtaattttctatgggctagttgctgcgttaactacaataatggggctgacatcctgaggttatagtgttcgattaccaagttctaatttattaagaggaatgcaagtcaacatgcatgcctagcccatttacttttaatgagtagtgtaacttctgttgcagaagtactaatgacagctttctatataaatttcacaggttgatttatcaaggacaacaattcgtcagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaaggtgaggaaactactcagcaaaattatacagtcatcattttgtgcacacttttagttatcagcaacattcgtatgtaggacgaatatatgatttgggaggacaagttatagcagcaaacagtgcccctgttataactcaccttgcagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggtgagttgttctagaataaaatttaaatttgaggtaactggataataagcatctaattatttataatattctctctatttcttgcatgcgtgttattcaataattcttacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactggtgagcatattttaaattcagtttggctggcattgaagctatagtatttcagttgccttattagtatgtatgatcttttgttcagatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaaggtatatatgttaataatatttctcctgttgcaaggtcttcttttttctcgtgataacattggtaatttcctgcattgcagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaaggtgagcaagaaataatggcgtaatatatctcaattattaattccaccttcatcaaatttcttagagaaattgtgcatatatttttccatacttgcagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttc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/>
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001187049.1 RefSeq:Os04g0473900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 4]]<br />
[[Category:Chromosome 4]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os04g0473900&diff=171565Os04g04739002014-05-24T04:21:47Z<p>Daweih: /* References */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
<br />
==Annotated Information==<br />
===Function===<br />
This gene is regarded as ''DWA1''.Putative ''DWA1'' plays important roles in drought resistance.The ''DWA1'' is composed of seven exons(Fig.1) The open reading fame (7,176 bp) encodes a protein consisting of 2,391 aa in ''japonica'' rice, Nipponbare, which is one of the 30 megaproteins (>2,300 aa) predicted in the rice genome<ref name="ref1"/>.<br />
<br />
===Mutation===<br />
[[File:图片3.png|right|thumb|200px|Fig.1 Identification of the drought-hypersensitive ''dwa1'' mutant in rice, in which functional complementation of ''dwa1'' restore the drought resistance(from reference<ref name="ref1" />)]]<br />
'''(1)'''There is an insertion of T-DNA in the sixth exon of ''DWA1'' (Fig.1A).<br><br />
'''(2)'''RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1B).<br><br />
'''(3)'''During the course of drought treatment, the mutant wilted and exhibited leaf rolling earlier than the WT.After severe drought treatment followed by rewatering, more than 90% of the WT plants survive, whereas the ''dwa1'' mutant plants are almost dead(Fig.1C and D).<br><br />
'''(4)'''Water loss rate of mutant is faster than WT(Fig.1E).<br><br />
'''(5)'''DWA1-functional complementation(DWA1-FC)lines which is inserted the full-length genomic fragment of ''DWA1'' into the ''dwa1'' mutant restore drought resistance(Fig.1F and G).<br><br />
'''(6)'''Plant height at the reproductive stage are also restored in the DWA1-FC lines(Fig.1H).<br />
<br />
===Expression===<br />
[[File:图片4.png|right|thumb|200px|Fig.2 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference<ref name="ref1" />)]]<br />
[[File:图片5.png|right|thumb|200px|Fig.3 Drought-induced expression of DWA1.(from reference<ref name="ref1" />)]]<br />
'''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.2A).<br><br />
'''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.2B 1-2), axillary bud (Fig.2B3), young panicle (Fig.2B4),spikelet (Fig.2B5), stem (Fig.2B6), young panicle (Fig.2B7), pistil (Fig.2B8), stamen (Fig.2B9), young embryo (Fig.2B10), SEM picture of rice leaf cuticle (Fig.2B11), cross-sections of stem (Fig.2B12) and leaf (Fig.2B13),and semithin sections of leaf (Fig.2B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.<br><br />
'''(3)'''What's more,after various phytohormones treatment,''DWA1'' expresses higher,such as abscisic acid, jasmonic acid, indole-3-acetic acid, and<br />
gibberellic acid (Fig.2C).<br><br />
'''(4)'''Under drought treatment,GUS staining shows that''DWA1'' is induced.For example,in Fig.3B,GUS staining in leaf before (1 and 3) and after (2 and 4) drought stress is shown.<br />
<br />
===Evolution===<br />
[[File:1(DWA1).png|right|thumb|200px|Fig.2 Unrooted phylogenetic tree and domain architecture of DWA1 protein orthologs in vascular plants and microbial relatives(from reference<ref name="ref1" />)]]<br />
At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region<ref name="ref1"/>(Fig.2).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi<ref name="ref2"/>,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain<ref name="ref1"/>.<br />
<br />
==Labs working on this gene==<br />
*National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan,China<br><br />
<br />
<br />
==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os04g0473900|<br />
Description = Adrenodoxin reductase family protein|<br />
Version = NM_001187049.1 GI:297723228 GeneID:9266980|<br />
Length = 9882 bp|<br />
Definition = Oryza sativa Japonica Group Os04g0473900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|<br />
AP = Chromosome 4:24097480..24107361|<br />
CDS = 24097480..24100068,24100784..24104053,24104151..24104329,24104410..24104769,24104855..24105296<br>,24105409..24105542,24105689..24105892,24107127..24107361|<br />
GCID = <gbrowseImage1><br />
name=NC_008397:24097480..24107361<br />
source=RiceChromosome04<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008397:24097480..24107361<br />
source=RiceChromosome04<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactgatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttctggctcagaaaggcatctcaattccagattcttctgatcaggttggacttgttgtgattgcagaggttagagagggtaaggcagtctctaaagaggttgttaacaacatcaaagcacgtgtggtggaggagcatggtgtagctgttgcatctgtgaagcttatcaagcctagaaccatatgcaagacaacatctgggaagatacgaagatttgaatgcatgaggcagtttgttgacaatactcttagtttggctaagggcaaccatgtatctaagaaaaagggtctttttcggtctctgacaacaggaactggcatggagtcaaagaggtccctcttgaggcaaactgttgacctcaccattagccattggcccaaaagccaagttaaaaactccaatgaaataactgagtttctgacacaaatagtgtcagagcacactggcatttcaaaggataaaatttctctaactgacagtctaccttcgtatggttttgattctattgcagtggtacgagcagcccagaagctctcagattttcttggtgttcctgttggggccattgacatctttacagcaagctgcatttcggagcttgcaagcttcttggagaatttggtgcataagtcacaaacacagttggcaccttggcccaaaagcaaagtgaaaaactccaaggtgattatcgagtttctgacaaagattgtatcagatcaaacagggattccaaaggataaaatttctccgatggatagcctaccttcatatggttttgattcaattgcggtggtccaagcagcacagaaactctccgattttctcggtgtacctgttggagccattgacatatttacagctggttgcatttctgagctagctaccttcttggagaatttggcgcataagtcacagtcacagttggcacctggtgcatcttgctatattgaggatgagactcaagtggatcccatggatgccattagtccagaattcagtgttttgggtacgggcatcctgcaactgctggctcttacttatgtctgttttgtgctgctactcccagcatatcttgctagttcgacatacatgagcattttctctacagtcagtctggtcagatcaccattgctgagctatcttagttctttggttatggcacctattgtgtggatcttttatatctcgctcacttctttatcactgtctatcttggggaagtccttcctacagccaaattatgttctgatccctgatgtttcaatatggtcagttgactttgtgaaatggtgggcactaaacaaagcacaagcattagctgcaaagatgctagctgtgcatctaaagggcacaattttcttaaattattggttcaagatgcaaggagctcggattggttcatctgttgtcatcgacacagtagacataaccgatccatccctgttgacggttgcagatggtgcagttcttgcagaaggcgctcttgttcagggccatgaagtctgtaatgaagtgctgagttttaggcctatctggattggctgtgaggcttcgattggtccttatgcggtgcttcagaagggaactgttgtggaagatggagctgttgttccaccattgcagaagactggggctgggaaatcaacacgtcggactagcagaacttctgtaagcataaagaaagaagcagccaaagcaaacatgatacttgaacacctcgttagcatctacgcagttggcatcctgggtgctctgtccggtgcaatcgtctacacactatacactcacttgtccggcaaagcggcttcgcctctgcatttctcctttgcatgcattgctggtgcgttccactggttaccagctgccatcactgcctatgctgttattgtccaggagacacccacaagtgcactgtcatttgctctcttcactgccttcgccgacctgagctatggtgtcatcctcagtattctcaccagtataaccagcagagcccttgctgcaaagcctggaaccaagcagaatggcattgcgagtttgatccaccggcgcataaccatctctgctcatgtcaggtttgccaagatgctatctggaacagaagccttctgcgtgtacctgaggcttctcggagcaaagattggcaggcattgctctatccgagccatcaatccggtggcgaatcctgagctgatcagcgtcggtgatggcgtccatctaggtgacttctgcaatattgttccagggttctactccaaggggggattcaccagtgcagagattaaggtccaggagaacaccgtggttggcagtggcagcttgctcctccctggctgtgtcctccaggagaatgtcatcctcggcgcgctctccgtcgcgccggagaacgctgttctccggcgtggcggcgtctacgtcgggtcacagtctccggccatggtgaagaacaccttgcttgacgaggatgaacggatcgaagagatggatcaggcatacaagaagattgttggcaacctcgcggcgaacttggccatcaccaccatgaacgtcaagtcgaggtacttccaccgcatcggcgtgagcggccgcggcgtcctgaggatgtacgaggagataccgtcgtttccccggcacaagatcttcgcctccggcaagtccttcccggtgatcgtccgccacagcaacagcctgagcgccgacgacgacgcgaggctcgacgcccgcggcgccgcggtgcggatcctctcggacaacgacggcgaggcgcctctcctcgacctcacactcaagagcggcaaggcgttctacgcgcgcaccatcgccgacttcgccacctggctggtctgcggcctgccggcgagggaggagcaggtcaagcgctcgccgcacatccgcgacgccgtctggggctctctccggagcaccgactcgtacaccgtgctccactactactccaacatatgccggctgctgcggttcgacgacggcagggagatgtacgccaagttcaagctccgtcctgctgatccagacgtccccgaggactccggcaaggtggtgccgaggggcatcctgccgccggagaccggcgcgatccccagggacgaggatgacacgcgccctctgctcttcctcgccgacgacttccgccggagagtgggctcaccggacggggtgcgctacgtgttccagctgcagctcagggaggtgcccaccgacgccgccgcccgcgacgtcgccctcgactgcacccggccgtgggacgaggccgagttcccttacatcgacgtcggcgaggtcagcatcggccgcaacctcccgacggaggagacggagaagctcgagttcaacccgttcctccggtgccccgaggtggacgtcatcccggcgacgtcgtgcgcgcagagcgcgtccatcgaccacggccgctcgctggtctacgagatatgccagcgcctgcggaacggcgagccgctgcccgcgtcgtggcgagctttcctggagcagtcggacaccaagatcgacctgtcaggatgcccggtcgctgccgccgcgacacccacaaggtctaacgccggcgacgccacgaaggtgacgctggccaggacgtggtaccaggcgctctgggccacgctgtgccagccgctgctgcaaacgctggtgccgtactctgtcctgggcctcgtcatcttcctcccgctcaggggcctcctcgccgtcgccgcggccacccgcttcccgctctactggctcctcccggcgttctgggccgcgtcgggcgtcgccgccatggccacgtgcgcggcggcgaagtgggcgctggtcggcagcagggtcgacggcgacacggtgcacatctggtcgccggccgtgttcctggacacggtctggcaggccgtgcgcgccgccacggcggagtacttcgccgagctgacgcccgggtcggcgccgttcgcggcgtggatgcgggtgatgggcgcctccgtgtcccccggcgacggcgtgtacgtggactccatgggcgcgctgctgaacccggagatggtgcgcctggagcgcggcgccgccgtcgggcgggacgcgctgctgttcggccacgtgtacgagggcgaggccgggaaggtgaagttcggcgccgtgtccgtcggggaggacgggttcgtcggcagccgcgccgtggccatgcccagcgtcacggtggacgacggcggctgcctcgccgcgctcgggctggccatgaagggggagacggtgaagcacagcatgtaa</cdnaseq>|<br />
AA = <aaseq>MEERRATSPSRHSFCRDALGRRGGESGGGLVYIYRSPLRSPLQR HGLNREWRLERWRWRWRWEAATWRQRAAAHARIGEMDPKRSIDDQFSKLHPSLPVDTR IGIVGAGPSGLSAAYALAKLGYRNVTLFEKCHTVSGMCESIDIEEELGSDFEEMDTHK LSLIDSQTGNIRDLEVAEDYVSMVSLTLKLQDEANKSGRAGLHALSGLASDPTHEFLK QNGINSMPKSVAYGYTASGYGFVQDMPYAFIQEFTRTSMAGKIRRFKHGYMSMWERLS KSLPFEVFCDTQVLNVKRNSCGANVTIKNNNGEKQVLEFDKIILSGAVAFKNSKTYRS SSLTDGESEVVELNNLERELFSKVQTIDYYTTVVKINGFEHIPKGFYYFGEFMEDPTT IGHPVAMQRFFADTNIFLFWSYGNSADIKGSFVAKCVTDVVSSMGGNVQKVILQRRFK YFPHVSSKDMKDGFYERLESQLQGFQNTYYVGGLLAFELTERNASYSIMAVCKHFAID GEGELTPYVKRLFPLSHNRNPSPPRDLGELEGVEFPDLPSLDGYLQYWGTHKVTEKNV IYTWINEEGKLMNRRTYQELHGNASYIAQKLLTSTKPVIKPGDRVLLIHLPGLEFIDA FFGCIRAGVIPVPVLPPDPMQSGGQALLKVENISKMCNAVAILSTSSYHAAVRAGYIK NIVTLAKRVQKCSAQWPDIPWIHTDSWIKNYRRSSDSFNSDTVLFTKPQPSDLCFLQF TSGSTGDAKGVMITHEGLIHNVKTMKKRYRSTSKTVLVSWLPQYHDMGLIGGLFTALV SGGTSVLFSPMIFIRNPLLWLQTINDYHGTHSAGPNFAFELVIRRLEAEKNKVYDLSS MVFLMIAAEPVRQKTVRRFIELTQPFGLSEGVLAPGYGLAENCVYVTCAFGECKPVFI DWQGRVCCGYVEQDDTDTLIRIVDPDSLTEHQEDGVEGEIWISSPSSGVGYWGNSEMS QRTFFNQLKNHPNKKFTRTGDLGRTIDGNLFITGRIKDLIIVAGRNIYSADVEKTVES SSEVLRPGCCAVVGIPEEVLAQKGISIPDSSDQVGLVVIAEVREGKAVSKEVVNNIKA RVVEEHGVAVASVKLIKPRTICKTTSGKIRRFECMRQFVDNTLSLAKGNHVSKKKGLF RSLTTGTGMESKRSLLRQTVDLTISHWPKSQVKNSNEITEFLTQIVSEHTGISKDKIS LTDSLPSYGFDSIAVVRAAQKLSDFLGVPVGAIDIFTASCISELASFLENLVHKSQTQ LAPWPKSKVKNSKVIIEFLTKIVSDQTGIPKDKISPMDSLPSYGFDSIAVVQAAQKLS DFLGVPVGAIDIFTAGCISELATFLENLAHKSQSQLAPGASCYIEDETQVDPMDAISP EFSVLGTGILQLLALTYVCFVLLLPAYLASSTYMSIFSTVSLVRSPLLSYLSSLVMAP IVWIFYISLTSLSLSILGKSFLQPNYVLIPDVSIWSVDFVKWWALNKAQALAAKMLAV HLKGTIFLNYWFKMQGARIGSSVVIDTVDITDPSLLTVADGAVLAEGALVQGHEVCNE VLSFRPIWIGCEASIGPYAVLQKGTVVEDGAVVPPLQKTGAGKSTRRTSRTSVSIKKE AAKANMILEHLVSIYAVGILGALSGAIVYTLYTHLSGKAASPLHFSFACIAGAFHWLP AAITAYAVIVQETPTSALSFALFTAFADLSYGVILSILTSITSRALAAKPGTKQNGIA SLIHRRITISAHVRFAKMLSGTEAFCVYLRLLGAKIGRHCSIRAINPVANPELISVGD GVHLGDFCNIVPGFYSKGGFTSAEIKVQENTVVGSGSLLLPGCVLQENVILGALSVAP ENAVLRRGGVYVGSQSPAMVKNTLLDEDERIEEMDQAYKKIVGNLAANLAITTMNVKS RYFHRIGVSGRGVLRMYEEIPSFPRHKIFASGKSFPVIVRHSNSLSADDDARLDARGA AVRILSDNDGEAPLLDLTLKSGKAFYARTIADFATWLVCGLPAREEQVKRSPHIRDAV WGSLRSTDSYTVLHYYSNICRLLRFDDGREMYAKFKLRPADPDVPEDSGKVVPRGILP PETGAIPRDEDDTRPLLFLADDFRRRVGSPDGVRYVFQLQLREVPTDAAARDVALDCT RPWDEAEFPYIDVGEVSIGRNLPTEETEKLEFNPFLRCPEVDVIPATSCAQSASIDHG RSLVYEICQRLRNGEPLPASWRAFLEQSDTKIDLSGCPVAAAATPTRSNAGDATKVTL ARTWYQALWATLCQPLLQTLVPYSVLGLVIFLPLRGLLAVAAATRFPLYWLLPAFWAA SGVAAMATCAAAKWALVGSRVDGDTVHIWSPAVFLDTVWQAVRAATAEYFAELTPGSA PFAAWMRVMGASVSPGDGVYVDSMGALLNPEMVRLERGAAVGRDALLFGHVYEGEAGK VKFGAVSVGEDGFVGSRAVAMPSVTVDDGGCLAALGLAMKGETVKHSM</aaseq>|<br />
DNA = <dnaseqindica>7294..9882#3309..6578#3033..3211#2593..2952#2066..2507#1820..1953#1470..1673#1..235#atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggaggtatggattccccttccccactcgctgcgtgatgaaccgatgaaccgtagtacttgatgatatgcctgatttgcttcggcattgtgccgcttctctgttagctgggagtttgtggcgtgtcgtttaactgaagtagagatgtgatgatgatctgaggtgagcgccattttttttttttggctaactctgtttgttctattcagtgaatatgagctctcttttttgtcccctttttatcattctgcttctatgggtcgcggagcgttcttggttggtgcagtgcaactgcaatgtcagtatgcatgccatagtaatgtgatggtgggatgtaatgctcctctgcttgctcgagctctttcttggttgtctatctatcttggatgatttgatcaatgtactacttattatctgctgatagtacccggggtccatttgcgctagtattgaacttcttcttctcttataatttgctgaaatcgcgagattatcgcttaggtttatcaatcagaacattatcatgtttatgcttcggttcgttcggtactggcacatgtcaaataagcggaaattctgctactttttcggcttaccatgaatggcacccaacatagcgtggttgttttgtcatgttgaactagtacaaatttttgcaccttaatatttgctttccgtgcatatccaattaacctgcccatcaaacttcttttcagatagctattattggggaaaaatattttcccagcttcttttatagtgactagactgtttatacatgcctgcgtaattgcttccttttgatgagctagtacattatagaccttgattttgttttgatggtacgggttggttattggtgtccatataatgaaattgcaacttgcgaccactccatgatagttacctggtcattccttgttggttaataagctattatttcaaatatgaatgttcatcctacttgtcatattatagacatttgtcacaattcaagtgaggaagaaattctgtaattttctatgggctagttgctgcgttaactacaataatggggctgacatcctgaggttatagtgttcgattaccaagttctaatttattaagaggaatgcaagtcaacatgcatgcctagcccatttacttttaatgagtagtgtaacttctgttgcagaagtactaatgacagctttctatataaatttcacaggttgatttatcaaggacaacaattcgtcagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaaggtgaggaaactactcagcaaaattatacagtcatcattttgtgcacacttttagttatcagcaacattcgtatgtaggacgaatatatgatttgggaggacaagttatagcagcaaacagtgcccctgttataactcaccttgcagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggtgagttgttctagaataaaatttaaatttgaggtaactggataataagcatctaattatttataatattctctctatttcttgcatgcgtgttattcaataattcttacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactggtgagcatattttaaattcagtttggctggcattgaagctatagtatttcagttgccttattagtatgtatgatcttttgttcagatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaaggtatatatgttaataatatttctcctgttgcaaggtcttcttttttctcgtgataacattggtaatttcctgcattgcagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaaggtgagcaagaaataatggcgtaatatatctcaattattaattccaccttcatcaaatttcttagagaaattgtgcatatatttttccatacttgcagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttc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/>
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001187049.1 RefSeq:Os04g0473900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 4]]<br />
[[Category:Chromosome 4]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os04g0473900&diff=171563Os04g04739002014-05-24T04:20:21Z<p>Daweih: /* Labs working on this gene */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
<br />
==Annotated Information==<br />
===Function===<br />
This gene is regarded as ''DWA1''.Putative ''DWA1'' plays important roles in drought resistance.The ''DWA1'' is composed of seven exons(Fig.1) The open reading fame (7,176 bp) encodes a protein consisting of 2,391 aa in ''japonica'' rice, Nipponbare, which is one of the 30 megaproteins (>2,300 aa) predicted in the rice genome<ref name="ref1"/>.<br />
<br />
===Mutation===<br />
[[File:图片3.png|right|thumb|200px|Fig.1 Identification of the drought-hypersensitive ''dwa1'' mutant in rice, in which functional complementation of ''dwa1'' restore the drought resistance(from reference<ref name="ref1" />)]]<br />
'''(1)'''There is an insertion of T-DNA in the sixth exon of ''DWA1'' (Fig.1A).<br><br />
'''(2)'''RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1B).<br><br />
'''(3)'''During the course of drought treatment, the mutant wilted and exhibited leaf rolling earlier than the WT.After severe drought treatment followed by rewatering, more than 90% of the WT plants survive, whereas the ''dwa1'' mutant plants are almost dead(Fig.1C and D).<br><br />
'''(4)'''Water loss rate of mutant is faster than WT(Fig.1E).<br><br />
'''(5)'''DWA1-functional complementation(DWA1-FC)lines which is inserted the full-length genomic fragment of ''DWA1'' into the ''dwa1'' mutant restore drought resistance(Fig.1F and G).<br><br />
'''(6)'''Plant height at the reproductive stage are also restored in the DWA1-FC lines(Fig.1H).<br />
<br />
===Expression===<br />
[[File:图片4.png|right|thumb|200px|Fig.2 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference<ref name="ref1" />)]]<br />
[[File:图片5.png|right|thumb|200px|Fig.3 Drought-induced expression of DWA1.(from reference<ref name="ref1" />)]]<br />
'''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.2A).<br><br />
'''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.2B 1-2), axillary bud (Fig.2B3), young panicle (Fig.2B4),spikelet (Fig.2B5), stem (Fig.2B6), young panicle (Fig.2B7), pistil (Fig.2B8), stamen (Fig.2B9), young embryo (Fig.2B10), SEM picture of rice leaf cuticle (Fig.2B11), cross-sections of stem (Fig.2B12) and leaf (Fig.2B13),and semithin sections of leaf (Fig.2B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.<br><br />
'''(3)'''What's more,after various phytohormones treatment,''DWA1'' expresses higher,such as abscisic acid, jasmonic acid, indole-3-acetic acid, and<br />
gibberellic acid (Fig.2C).<br><br />
'''(4)'''Under drought treatment,GUS staining shows that''DWA1'' is induced.For example,in Fig.3B,GUS staining in leaf before (1 and 3) and after (2 and 4) drought stress is shown.<br />
<br />
===Evolution===<br />
[[File:1(DWA1).png|right|thumb|200px|Fig.2 Unrooted phylogenetic tree and domain architecture of DWA1 protein orthologs in vascular plants and microbial relatives(from reference<ref name="ref1" />)]]<br />
At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region<ref name="ref1"/>(Fig.2).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi<ref name="ref2"/>,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain<ref name="ref1"/>.<br />
<br />
==Labs working on this gene==<br />
*National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan,China<br><br />
<br />
<br />
==References==<br />
<references><br />
<ref name="ref1">Xiaoyi Zhu, Lizhong Xiong.（2013）Putative megaenzyme DWA1 plays essential roles in drought resistance by regulating stress-induced wax<br />
deposition in rice.PANS 110(44): 17790-17795.</ref><br />
<ref name="ref2">Marahiel MA, Stachelhaus T, Mootz HD.(1997) Modular peptide synthetases involved in nonribosomal peptide synthesis.Chem Rev 97(7):2651–2674.</ref><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os04g0473900|<br />
Description = Adrenodoxin reductase family protein|<br />
Version = NM_001187049.1 GI:297723228 GeneID:9266980|<br />
Length = 9882 bp|<br />
Definition = Oryza sativa Japonica Group Os04g0473900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|<br />
AP = Chromosome 4:24097480..24107361|<br />
CDS = 24097480..24100068,24100784..24104053,24104151..24104329,24104410..24104769,24104855..24105296<br>,24105409..24105542,24105689..24105892,24107127..24107361|<br />
GCID = <gbrowseImage1><br />
name=NC_008397:24097480..24107361<br />
source=RiceChromosome04<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008397:24097480..24107361<br />
source=RiceChromosome04<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactgatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttctggctcagaaaggcatctcaattccagattcttctgatcaggttggacttgttgtgattgcagaggttagagagggtaaggcagtctctaaagaggttgttaacaacatcaaagcacgtgtggtggaggagcatggtgtagctgttgcatctgtgaagcttatcaagcctagaaccatatgcaagacaacatctgggaagatacgaagatttgaatgcatgaggcagtttgttgacaatactcttagtttggctaagggcaaccatgtatctaagaaaaagggtctttttcggtctctgacaacaggaactggcatggagtcaaagaggtccctcttgaggcaaactgttgacctcaccattagccattggcccaaaagccaagttaaaaactccaatgaaataactgagtttctgacacaaatagtgtcagagcacactggcatttcaaaggataaaatttctctaactgacagtctaccttcgtatggttttgattctattgcagtggtacgagcagcccagaagctctcagattttcttggtgttcctgttggggccattgacatctttacagcaagctgcatttcggagcttgcaagcttcttggagaatttggtgcataagtcacaaacacagttggcaccttggcccaaaagcaaagtgaaaaactccaaggtgattatcgagtttctgacaaagattgtatcagatcaaacagggattccaaaggataaaatttctccgatggatagcctaccttcatatggttttgattcaattgcggtggtccaagcagcacagaaactctccgattttctcggtgtacctgttggagccattgacatatttacagctggttgcatttctgagctagctaccttcttggagaatttggcgcataagtcacagtcacagttggcacctggtgcatcttgctatattgaggatgagactcaagtggatcccatggatgccattagtccagaattcagtgttttgggtacgggcatcctgcaactgctggctcttacttatgtctgttttgtgctgctactcccagcatatcttgctagttcgacatacatgagcattttctctacagtcagtctggtcagatcaccattgctgagctatcttagttctttggttatggcacctattgtgtggatcttttatatctcgctcacttctttatcactgtctatcttggggaagtccttcctacagccaaattatgttctgatccctgatgtttcaatatggtcagttgactttgtgaaatggtgggcactaaacaaagcacaagcattagctgcaaagatgctagctgtgcatctaaagggcacaattttcttaaattattggttcaagatgcaaggagctcggattggttcatctgttgtcatcgacacagtagacataaccgatccatccctgttgacggttgcagatggtgcagttcttgcagaaggcgctcttgttcagggccatgaagtctgtaatgaagtgctgagttttaggcctatctggattggctgtgaggcttcgattggtccttatgcggtgcttcagaagggaactgttgtggaagatggagctgttgttccaccattgcagaagactggggctgggaaatcaacacgtcggactagcagaacttctgtaagcataaagaaagaagcagccaaagcaaacatgatacttgaacacctcgttagcatctacgcagttggcatcctgggtgctctgtccggtgcaatcgtctacacactatacactcacttgtccggcaaagcggcttcgcctctgcatttctcctttgcatgcattgctggtgcgttccactggttaccagctgccatcactgcctatgctgttattgtccaggagacacccacaagtgcactgtcatttgctctcttcactgccttcgccgacctgagctatggtgtcatcctcagtattctcaccagtataaccagcagagcccttgctgcaaagcctggaaccaagcagaatggcattgcgagtttgatccaccggcgcataaccatctctgctcatgtcaggtttgccaagatgctatctggaacagaagccttctgcgtgtacctgaggcttctcggagcaaagattggcaggcattgctctatccgagccatcaatccggtggcgaatcctgagctgatcagcgtcggtgatggcgtccatctaggtgacttctgcaatattgttccagggttctactccaaggggggattcaccagtgcagagattaaggtccaggagaacaccgtggttggcagtggcagcttgctcctccctggctgtgtcctccaggagaatgtcatcctcggcgcgctctccgtcgcgccggagaacgctgttctccggcgtggcggcgtctacgtcgggtcacagtctccggccatggtgaagaacaccttgcttgacgaggatgaacggatcgaagagatggatcaggcatacaagaagattgttggcaacctcgcggcgaacttggccatcaccaccatgaacgtcaagtcgaggtacttccaccgcatcggcgtgagcggccgcggcgtcctgaggatgtacgaggagataccgtcgtttccccggcacaagatcttcgcctccggcaagtccttcccggtgatcgtccgccacagcaacagcctgagcgccgacgacgacgcgaggctcgacgcccgcggcgccgcggtgcggatcctctcggacaacgacggcgaggcgcctctcctcgacctcacactcaagagcggcaaggcgttctacgcgcgcaccatcgccgacttcgccacctggctggtctgcggcctgccggcgagggaggagcaggtcaagcgctcgccgcacatccgcgacgccgtctggggctctctccggagcaccgactcgtacaccgtgctccactactactccaacatatgccggctgctgcggttcgacgacggcagggagatgtacgccaagttcaagctccgtcctgctgatccagacgtccccgaggactccggcaaggtggtgccgaggggcatcctgccgccggagaccggcgcgatccccagggacgaggatgacacgcgccctctgctcttcctcgccgacgacttccgccggagagtgggctcaccggacggggtgcgctacgtgttccagctgcagctcagggaggtgcccaccgacgccgccgcccgcgacgtcgccctcgactgcacccggccgtgggacgaggccgagttcccttacatcgacgtcggcgaggtcagcatcggccgcaacctcccgacggaggagacggagaagctcgagttcaacccgttcctccggtgccccgaggtggacgtcatcccggcgacgtcgtgcgcgcagagcgcgtccatcgaccacggccgctcgctggtctacgagatatgccagcgcctgcggaacggcgagccgctgcccgcgtcgtggcgagctttcctggagcagtcggacaccaagatcgacctgtcaggatgcccggtcgctgccgccgcgacacccacaaggtctaacgccggcgacgccacgaaggtgacgctggccaggacgtggtaccaggcgctctgggccacgctgtgccagccgctgctgcaaacgctggtgccgtactctgtcctgggcctcgtcatcttcctcccgctcaggggcctcctcgccgtcgccgcggccacccgcttcccgctctactggctcctcccggcgttctgggccgcgtcgggcgtcgccgccatggccacgtgcgcggcggcgaagtgggcgctggtcggcagcagggtcgacggcgacacggtgcacatctggtcgccggccgtgttcctggacacggtctggcaggccgtgcgcgccgccacggcggagtacttcgccgagctgacgcccgggtcggcgccgttcgcggcgtggatgcgggtgatgggcgcctccgtgtcccccggcgacggcgtgtacgtggactccatgggcgcgctgctgaacccggagatggtgcgcctggagcgcggcgccgccgtcgggcgggacgcgctgctgttcggccacgtgtacgagggcgaggccgggaaggtgaagttcggcgccgtgtccgtcggggaggacgggttcgtcggcagccgcgccgtggccatgcccagcgtcacggtggacgacggcggctgcctcgccgcgctcgggctggccatgaagggggagacggtgaagcacagcatgtaa</cdnaseq>|<br />
AA = <aaseq>MEERRATSPSRHSFCRDALGRRGGESGGGLVYIYRSPLRSPLQR HGLNREWRLERWRWRWRWEAATWRQRAAAHARIGEMDPKRSIDDQFSKLHPSLPVDTR IGIVGAGPSGLSAAYALAKLGYRNVTLFEKCHTVSGMCESIDIEEELGSDFEEMDTHK LSLIDSQTGNIRDLEVAEDYVSMVSLTLKLQDEANKSGRAGLHALSGLASDPTHEFLK QNGINSMPKSVAYGYTASGYGFVQDMPYAFIQEFTRTSMAGKIRRFKHGYMSMWERLS KSLPFEVFCDTQVLNVKRNSCGANVTIKNNNGEKQVLEFDKIILSGAVAFKNSKTYRS SSLTDGESEVVELNNLERELFSKVQTIDYYTTVVKINGFEHIPKGFYYFGEFMEDPTT IGHPVAMQRFFADTNIFLFWSYGNSADIKGSFVAKCVTDVVSSMGGNVQKVILQRRFK YFPHVSSKDMKDGFYERLESQLQGFQNTYYVGGLLAFELTERNASYSIMAVCKHFAID GEGELTPYVKRLFPLSHNRNPSPPRDLGELEGVEFPDLPSLDGYLQYWGTHKVTEKNV IYTWINEEGKLMNRRTYQELHGNASYIAQKLLTSTKPVIKPGDRVLLIHLPGLEFIDA FFGCIRAGVIPVPVLPPDPMQSGGQALLKVENISKMCNAVAILSTSSYHAAVRAGYIK NIVTLAKRVQKCSAQWPDIPWIHTDSWIKNYRRSSDSFNSDTVLFTKPQPSDLCFLQF TSGSTGDAKGVMITHEGLIHNVKTMKKRYRSTSKTVLVSWLPQYHDMGLIGGLFTALV SGGTSVLFSPMIFIRNPLLWLQTINDYHGTHSAGPNFAFELVIRRLEAEKNKVYDLSS MVFLMIAAEPVRQKTVRRFIELTQPFGLSEGVLAPGYGLAENCVYVTCAFGECKPVFI DWQGRVCCGYVEQDDTDTLIRIVDPDSLTEHQEDGVEGEIWISSPSSGVGYWGNSEMS QRTFFNQLKNHPNKKFTRTGDLGRTIDGNLFITGRIKDLIIVAGRNIYSADVEKTVES SSEVLRPGCCAVVGIPEEVLAQKGISIPDSSDQVGLVVIAEVREGKAVSKEVVNNIKA RVVEEHGVAVASVKLIKPRTICKTTSGKIRRFECMRQFVDNTLSLAKGNHVSKKKGLF RSLTTGTGMESKRSLLRQTVDLTISHWPKSQVKNSNEITEFLTQIVSEHTGISKDKIS LTDSLPSYGFDSIAVVRAAQKLSDFLGVPVGAIDIFTASCISELASFLENLVHKSQTQ LAPWPKSKVKNSKVIIEFLTKIVSDQTGIPKDKISPMDSLPSYGFDSIAVVQAAQKLS DFLGVPVGAIDIFTAGCISELATFLENLAHKSQSQLAPGASCYIEDETQVDPMDAISP EFSVLGTGILQLLALTYVCFVLLLPAYLASSTYMSIFSTVSLVRSPLLSYLSSLVMAP IVWIFYISLTSLSLSILGKSFLQPNYVLIPDVSIWSVDFVKWWALNKAQALAAKMLAV HLKGTIFLNYWFKMQGARIGSSVVIDTVDITDPSLLTVADGAVLAEGALVQGHEVCNE VLSFRPIWIGCEASIGPYAVLQKGTVVEDGAVVPPLQKTGAGKSTRRTSRTSVSIKKE AAKANMILEHLVSIYAVGILGALSGAIVYTLYTHLSGKAASPLHFSFACIAGAFHWLP AAITAYAVIVQETPTSALSFALFTAFADLSYGVILSILTSITSRALAAKPGTKQNGIA SLIHRRITISAHVRFAKMLSGTEAFCVYLRLLGAKIGRHCSIRAINPVANPELISVGD GVHLGDFCNIVPGFYSKGGFTSAEIKVQENTVVGSGSLLLPGCVLQENVILGALSVAP ENAVLRRGGVYVGSQSPAMVKNTLLDEDERIEEMDQAYKKIVGNLAANLAITTMNVKS RYFHRIGVSGRGVLRMYEEIPSFPRHKIFASGKSFPVIVRHSNSLSADDDARLDARGA AVRILSDNDGEAPLLDLTLKSGKAFYARTIADFATWLVCGLPAREEQVKRSPHIRDAV WGSLRSTDSYTVLHYYSNICRLLRFDDGREMYAKFKLRPADPDVPEDSGKVVPRGILP PETGAIPRDEDDTRPLLFLADDFRRRVGSPDGVRYVFQLQLREVPTDAAARDVALDCT RPWDEAEFPYIDVGEVSIGRNLPTEETEKLEFNPFLRCPEVDVIPATSCAQSASIDHG RSLVYEICQRLRNGEPLPASWRAFLEQSDTKIDLSGCPVAAAATPTRSNAGDATKVTL ARTWYQALWATLCQPLLQTLVPYSVLGLVIFLPLRGLLAVAAATRFPLYWLLPAFWAA SGVAAMATCAAAKWALVGSRVDGDTVHIWSPAVFLDTVWQAVRAATAEYFAELTPGSA PFAAWMRVMGASVSPGDGVYVDSMGALLNPEMVRLERGAAVGRDALLFGHVYEGEAGK VKFGAVSVGEDGFVGSRAVAMPSVTVDDGGCLAALGLAMKGETVKHSM</aaseq>|<br />
DNA = <dnaseqindica>7294..9882#3309..6578#3033..3211#2593..2952#2066..2507#1820..1953#1470..1673#1..235#atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggaggtatggattccccttccccactcgctgcgtgatgaaccgatgaaccgtagtacttgatgatatgcctgatttgcttcggcattgtgccgcttctctgttagctgggagtttgtggcgtgtcgtttaactgaagtagagatgtgatgatgatctgaggtgagcgccattttttttttttggctaactctgtttgttctattcagtgaatatgagctctcttttttgtcccctttttatcattctgcttctatgggtcgcggagcgttcttggttggtgcagtgcaactgcaatgtcagtatgcatgccatagtaatgtgatggtgggatgtaatgctcctctgcttgctcgagctctttcttggttgtctatctatcttggatgatttgatcaatgtactacttattatctgctgatagtacccggggtccatttgcgctagtattgaacttcttcttctcttataatttgctgaaatcgcgagattatcgcttaggtttatcaatcagaacattatcatgtttatgcttcggttcgttcggtactggcacatgtcaaataagcggaaattctgctactttttcggcttaccatgaatggcacccaacatagcgtggttgttttgtcatgttgaactagtacaaatttttgcaccttaatatttgctttccgtgcatatccaattaacctgcccatcaaacttcttttcagatagctattattggggaaaaatattttcccagcttcttttatagtgactagactgtttatacatgcctgcgtaattgcttccttttgatgagctagtacattatagaccttgattttgttttgatggtacgggttggttattggtgtccatataatgaaattgcaacttgcgaccactccatgatagttacctggtcattccttgttggttaataagctattatttcaaatatgaatgttcatcctacttgtcatattatagacatttgtcacaattcaagtgaggaagaaattctgtaattttctatgggctagttgctgcgttaactacaataatggggctgacatcctgaggttatagtgttcgattaccaagttctaatttattaagaggaatgcaagtcaacatgcatgcctagcccatttacttttaatgagtagtgtaacttctgttgcagaagtactaatgacagctttctatataaatttcacaggttgatttatcaaggacaacaattcgtcagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaaggtgaggaaactactcagcaaaattatacagtcatcattttgtgcacacttttagttatcagcaacattcgtatgtaggacgaatatatgatttgggaggacaagttatagcagcaaacagtgcccctgttataactcaccttgcagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggtgagttgttctagaataaaatttaaatttgaggtaactggataataagcatctaattatttataatattctctctatttcttgcatgcgtgttattcaataattcttacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactggtgagcatattttaaattcagtttggctggcattgaagctatagtatttcagttgccttattagtatgtatgatcttttgttcagatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaaggtatatatgttaataatatttctcctgttgcaaggtcttcttttttctcgtgataacattggtaatttcctgcattgcagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaaggtgagcaagaaataatggcgtaatatatctcaattattaattccaccttcatcaaatttcttagagaaattgtgcatatatttttccatacttgcagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttc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/>
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001187049.1 RefSeq:Os04g0473900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 4]]<br />
[[Category:Chromosome 4]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os04g0473900&diff=171562Os04g04739002014-05-24T04:19:53Z<p>Daweih: /* References */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
<br />
==Annotated Information==<br />
===Function===<br />
This gene is regarded as ''DWA1''.Putative ''DWA1'' plays important roles in drought resistance.The ''DWA1'' is composed of seven exons(Fig.1) The open reading fame (7,176 bp) encodes a protein consisting of 2,391 aa in ''japonica'' rice, Nipponbare, which is one of the 30 megaproteins (>2,300 aa) predicted in the rice genome<ref name="ref1"/>.<br />
<br />
===Mutation===<br />
[[File:图片3.png|right|thumb|200px|Fig.1 Identification of the drought-hypersensitive ''dwa1'' mutant in rice, in which functional complementation of ''dwa1'' restore the drought resistance(from reference<ref name="ref1" />)]]<br />
'''(1)'''There is an insertion of T-DNA in the sixth exon of ''DWA1'' (Fig.1A).<br><br />
'''(2)'''RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1B).<br><br />
'''(3)'''During the course of drought treatment, the mutant wilted and exhibited leaf rolling earlier than the WT.After severe drought treatment followed by rewatering, more than 90% of the WT plants survive, whereas the ''dwa1'' mutant plants are almost dead(Fig.1C and D).<br><br />
'''(4)'''Water loss rate of mutant is faster than WT(Fig.1E).<br><br />
'''(5)'''DWA1-functional complementation(DWA1-FC)lines which is inserted the full-length genomic fragment of ''DWA1'' into the ''dwa1'' mutant restore drought resistance(Fig.1F and G).<br><br />
'''(6)'''Plant height at the reproductive stage are also restored in the DWA1-FC lines(Fig.1H).<br />
<br />
===Expression===<br />
[[File:图片4.png|right|thumb|200px|Fig.2 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference<ref name="ref1" />)]]<br />
[[File:图片5.png|right|thumb|200px|Fig.3 Drought-induced expression of DWA1.(from reference<ref name="ref1" />)]]<br />
'''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.2A).<br><br />
'''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.2B 1-2), axillary bud (Fig.2B3), young panicle (Fig.2B4),spikelet (Fig.2B5), stem (Fig.2B6), young panicle (Fig.2B7), pistil (Fig.2B8), stamen (Fig.2B9), young embryo (Fig.2B10), SEM picture of rice leaf cuticle (Fig.2B11), cross-sections of stem (Fig.2B12) and leaf (Fig.2B13),and semithin sections of leaf (Fig.2B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.<br><br />
'''(3)'''What's more,after various phytohormones treatment,''DWA1'' expresses higher,such as abscisic acid, jasmonic acid, indole-3-acetic acid, and<br />
gibberellic acid (Fig.2C).<br><br />
'''(4)'''Under drought treatment,GUS staining shows that''DWA1'' is induced.For example,in Fig.3B,GUS staining in leaf before (1 and 3) and after (2 and 4) drought stress is shown.<br />
<br />
===Evolution===<br />
[[File:1(DWA1).png|right|thumb|200px|Fig.2 Unrooted phylogenetic tree and domain architecture of DWA1 protein orthologs in vascular plants and microbial relatives(from reference<ref name="ref1" />)]]<br />
At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region<ref name="ref1"/>(Fig.2).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi<ref name="ref2"/>,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain<ref name="ref1"/>.<br />
<br />
==Labs working on this gene==<br />
*National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan,China<br><br />
<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os04g0473900|<br />
Description = Adrenodoxin reductase family protein|<br />
Version = NM_001187049.1 GI:297723228 GeneID:9266980|<br />
Length = 9882 bp|<br />
Definition = Oryza sativa Japonica Group Os04g0473900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|<br />
AP = Chromosome 4:24097480..24107361|<br />
CDS = 24097480..24100068,24100784..24104053,24104151..24104329,24104410..24104769,24104855..24105296<br>,24105409..24105542,24105689..24105892,24107127..24107361|<br />
GCID = <gbrowseImage1><br />
name=NC_008397:24097480..24107361<br />
source=RiceChromosome04<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008397:24097480..24107361<br />
source=RiceChromosome04<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactgatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttctggctcagaaaggcatctcaattccagattcttctgatcaggttggacttgttgtgattgcagaggttagagagggtaaggcagtctctaaagaggttgttaacaacatcaaagcacgtgtggtggaggagcatggtgtagctgttgcatctgtgaagcttatcaagcctagaaccatatgcaagacaacatctgggaagatacgaagatttgaatgcatgaggcagtttgttgacaatactcttagtttggctaagggcaaccatgtatctaagaaaaagggtctttttcggtctctgacaacaggaactggcatggagtcaaagaggtccctcttgaggcaaactgttgacctcaccattagccattggcccaaaagccaagttaaaaactccaatgaaataactgagtttctgacacaaatagtgtcagagcacactggcatttcaaaggataaaatttctctaactgacagtctaccttcgtatggttttgattctattgcagtggtacgagcagcccagaagctctcagattttcttggtgttcctgttggggccattgacatctttacagcaagctgcatttcggagcttgcaagcttcttggagaatttggtgcataagtcacaaacacagttggcaccttggcccaaaagcaaagtgaaaaactccaaggtgattatcgagtttctgacaaagattgtatcagatcaaacagggattccaaaggataaaatttctccgatggatagcctaccttcatatggttttgattcaattgcggtggtccaagcagcacagaaactctccgattttctcggtgtacctgttggagccattgacatatttacagctggttgcatttctgagctagctaccttcttggagaatttggcgcataagtcacagtcacagttggcacctggtgcatcttgctatattgaggatgagactcaagtggatcccatggatgccattagtccagaattcagtgttttgggtacgggcatcctgcaactgctggctcttacttatgtctgttttgtgctgctactcccagcatatcttgctagttcgacatacatgagcattttctctacagtcagtctggtcagatcaccattgctgagctatcttagttctttggttatggcacctattgtgtggatcttttatatctcgctcacttctttatcactgtctatcttggggaagtccttcctacagccaaattatgttctgatccctgatgtttcaatatggtcagttgactttgtgaaatggtgggcactaaacaaagcacaagcattagctgcaaagatgctagctgtgcatctaaagggcacaattttcttaaattattggttcaagatgcaaggagctcggattggttcatctgttgtcatcgacacagtagacataaccgatccatccctgttgacggttgcagatggtgcagttcttgcagaaggcgctcttgttcagggccatgaagtctgtaatgaagtgctgagttttaggcctatctggattggctgtgaggcttcgattggtccttatgcggtgcttcagaagggaactgttgtggaagatggagctgttgttccaccattgcagaagactggggctgggaaatcaacacgtcggactagcagaacttctgtaagcataaagaaagaagcagccaaagcaaacatgatacttgaacacctcgttagcatctacgcagttggcatcctgggtgctctgtccggtgcaatcgtctacacactatacactcacttgtccggcaaagcggcttcgcctctgcatttctcctttgcatgcattgctggtgcgttccactggttaccagctgccatcactgcctatgctgttattgtccaggagacacccacaagtgcactgtcatttgctctcttcactgccttcgccgacctgagctatggtgtcatcctcagtattctcaccagtataaccagcagagcccttgctgcaaagcctggaaccaagcagaatggcattgcgagtttgatccaccggcgcataaccatctctgctcatgtcaggtttgccaagatgctatctggaacagaagccttctgcgtgtacctgaggcttctcggagcaaagattggcaggcattgctctatccgagccatcaatccggtggcgaatcctgagctgatcagcgtcggtgatggcgtccatctaggtgacttctgcaatattgttccagggttctactccaaggggggattcaccagtgcagagattaaggtccaggagaacaccgtggttggcagtggcagcttgctcctccctggctgtgtcctccaggagaatgtcatcctcggcgcgctctccgtcgcgccggagaacgctgttctccggcgtggcggcgtctacgtcgggtcacagtctccggccatggtgaagaacaccttgcttgacgaggatgaacggatcgaagagatggatcaggcatacaagaagattgttggcaacctcgcggcgaacttggccatcaccaccatgaacgtcaagtcgaggtacttccaccgcatcggcgtgagcggccgcggcgtcctgaggatgtacgaggagataccgtcgtttccccggcacaagatcttcgcctccggcaagtccttcccggtgatcgtccgccacagcaacagcctgagcgccgacgacgacgcgaggctcgacgcccgcggcgccgcggtgcggatcctctcggacaacgacggcgaggcgcctctcctcgacctcacactcaagagcggcaaggcgttctacgcgcgcaccatcgccgacttcgccacctggctggtctgcggcctgccggcgagggaggagcaggtcaagcgctcgccgcacatccgcgacgccgtctggggctctctccggagcaccgactcgtacaccgtgctccactactactccaacatatgccggctgctgcggttcgacgacggcagggagatgtacgccaagttcaagctccgtcctgctgatccagacgtccccgaggactccggcaaggtggtgccgaggggcatcctgccgccggagaccggcgcgatccccagggacgaggatgacacgcgccctctgctcttcctcgccgacgacttccgccggagagtgggctcaccggacggggtgcgctacgtgttccagctgcagctcagggaggtgcccaccgacgccgccgcccgcgacgtcgccctcgactgcacccggccgtgggacgaggccgagttcccttacatcgacgtcggcgaggtcagcatcggccgcaacctcccgacggaggagacggagaagctcgagttcaacccgttcctccggtgccccgaggtggacgtcatcccggcgacgtcgtgcgcgcagagcgcgtccatcgaccacggccgctcgctggtctacgagatatgccagcgcctgcggaacggcgagccgctgcccgcgtcgtggcgagctttcctggagcagtcggacaccaagatcgacctgtcaggatgcccggtcgctgccgccgcgacacccacaaggtctaacgccggcgacgccacgaaggtgacgctggccaggacgtggtaccaggcgctctgggccacgctgtgccagccgctgctgcaaacgctggtgccgtactctgtcctgggcctcgtcatcttcctcccgctcaggggcctcctcgccgtcgccgcggccacccgcttcccgctctactggctcctcccggcgttctgggccgcgtcgggcgtcgccgccatggccacgtgcgcggcggcgaagtgggcgctggtcggcagcagggtcgacggcgacacggtgcacatctggtcgccggccgtgttcctggacacggtctggcaggccgtgcgcgccgccacggcggagtacttcgccgagctgacgcccgggtcggcgccgttcgcggcgtggatgcgggtgatgggcgcctccgtgtcccccggcgacggcgtgtacgtggactccatgggcgcgctgctgaacccggagatggtgcgcctggagcgcggcgccgccgtcgggcgggacgcgctgctgttcggccacgtgtacgagggcgaggccgggaaggtgaagttcggcgccgtgtccgtcggggaggacgggttcgtcggcagccgcgccgtggccatgcccagcgtcacggtggacgacggcggctgcctcgccgcgctcgggctggccatgaagggggagacggtgaagcacagcatgtaa</cdnaseq>|<br />
AA = <aaseq>MEERRATSPSRHSFCRDALGRRGGESGGGLVYIYRSPLRSPLQR HGLNREWRLERWRWRWRWEAATWRQRAAAHARIGEMDPKRSIDDQFSKLHPSLPVDTR IGIVGAGPSGLSAAYALAKLGYRNVTLFEKCHTVSGMCESIDIEEELGSDFEEMDTHK LSLIDSQTGNIRDLEVAEDYVSMVSLTLKLQDEANKSGRAGLHALSGLASDPTHEFLK QNGINSMPKSVAYGYTASGYGFVQDMPYAFIQEFTRTSMAGKIRRFKHGYMSMWERLS KSLPFEVFCDTQVLNVKRNSCGANVTIKNNNGEKQVLEFDKIILSGAVAFKNSKTYRS SSLTDGESEVVELNNLERELFSKVQTIDYYTTVVKINGFEHIPKGFYYFGEFMEDPTT IGHPVAMQRFFADTNIFLFWSYGNSADIKGSFVAKCVTDVVSSMGGNVQKVILQRRFK YFPHVSSKDMKDGFYERLESQLQGFQNTYYVGGLLAFELTERNASYSIMAVCKHFAID GEGELTPYVKRLFPLSHNRNPSPPRDLGELEGVEFPDLPSLDGYLQYWGTHKVTEKNV IYTWINEEGKLMNRRTYQELHGNASYIAQKLLTSTKPVIKPGDRVLLIHLPGLEFIDA FFGCIRAGVIPVPVLPPDPMQSGGQALLKVENISKMCNAVAILSTSSYHAAVRAGYIK NIVTLAKRVQKCSAQWPDIPWIHTDSWIKNYRRSSDSFNSDTVLFTKPQPSDLCFLQF TSGSTGDAKGVMITHEGLIHNVKTMKKRYRSTSKTVLVSWLPQYHDMGLIGGLFTALV SGGTSVLFSPMIFIRNPLLWLQTINDYHGTHSAGPNFAFELVIRRLEAEKNKVYDLSS MVFLMIAAEPVRQKTVRRFIELTQPFGLSEGVLAPGYGLAENCVYVTCAFGECKPVFI DWQGRVCCGYVEQDDTDTLIRIVDPDSLTEHQEDGVEGEIWISSPSSGVGYWGNSEMS QRTFFNQLKNHPNKKFTRTGDLGRTIDGNLFITGRIKDLIIVAGRNIYSADVEKTVES SSEVLRPGCCAVVGIPEEVLAQKGISIPDSSDQVGLVVIAEVREGKAVSKEVVNNIKA RVVEEHGVAVASVKLIKPRTICKTTSGKIRRFECMRQFVDNTLSLAKGNHVSKKKGLF RSLTTGTGMESKRSLLRQTVDLTISHWPKSQVKNSNEITEFLTQIVSEHTGISKDKIS LTDSLPSYGFDSIAVVRAAQKLSDFLGVPVGAIDIFTASCISELASFLENLVHKSQTQ LAPWPKSKVKNSKVIIEFLTKIVSDQTGIPKDKISPMDSLPSYGFDSIAVVQAAQKLS DFLGVPVGAIDIFTAGCISELATFLENLAHKSQSQLAPGASCYIEDETQVDPMDAISP EFSVLGTGILQLLALTYVCFVLLLPAYLASSTYMSIFSTVSLVRSPLLSYLSSLVMAP IVWIFYISLTSLSLSILGKSFLQPNYVLIPDVSIWSVDFVKWWALNKAQALAAKMLAV HLKGTIFLNYWFKMQGARIGSSVVIDTVDITDPSLLTVADGAVLAEGALVQGHEVCNE VLSFRPIWIGCEASIGPYAVLQKGTVVEDGAVVPPLQKTGAGKSTRRTSRTSVSIKKE AAKANMILEHLVSIYAVGILGALSGAIVYTLYTHLSGKAASPLHFSFACIAGAFHWLP AAITAYAVIVQETPTSALSFALFTAFADLSYGVILSILTSITSRALAAKPGTKQNGIA SLIHRRITISAHVRFAKMLSGTEAFCVYLRLLGAKIGRHCSIRAINPVANPELISVGD GVHLGDFCNIVPGFYSKGGFTSAEIKVQENTVVGSGSLLLPGCVLQENVILGALSVAP ENAVLRRGGVYVGSQSPAMVKNTLLDEDERIEEMDQAYKKIVGNLAANLAITTMNVKS RYFHRIGVSGRGVLRMYEEIPSFPRHKIFASGKSFPVIVRHSNSLSADDDARLDARGA AVRILSDNDGEAPLLDLTLKSGKAFYARTIADFATWLVCGLPAREEQVKRSPHIRDAV WGSLRSTDSYTVLHYYSNICRLLRFDDGREMYAKFKLRPADPDVPEDSGKVVPRGILP PETGAIPRDEDDTRPLLFLADDFRRRVGSPDGVRYVFQLQLREVPTDAAARDVALDCT RPWDEAEFPYIDVGEVSIGRNLPTEETEKLEFNPFLRCPEVDVIPATSCAQSASIDHG RSLVYEICQRLRNGEPLPASWRAFLEQSDTKIDLSGCPVAAAATPTRSNAGDATKVTL ARTWYQALWATLCQPLLQTLVPYSVLGLVIFLPLRGLLAVAAATRFPLYWLLPAFWAA SGVAAMATCAAAKWALVGSRVDGDTVHIWSPAVFLDTVWQAVRAATAEYFAELTPGSA PFAAWMRVMGASVSPGDGVYVDSMGALLNPEMVRLERGAAVGRDALLFGHVYEGEAGK VKFGAVSVGEDGFVGSRAVAMPSVTVDDGGCLAALGLAMKGETVKHSM</aaseq>|<br />
DNA = <dnaseqindica>7294..9882#3309..6578#3033..3211#2593..2952#2066..2507#1820..1953#1470..1673#1..235#atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggaggtatggattccccttccccactcgctgcgtgatgaaccgatgaaccgtagtacttgatgatatgcctgatttgcttcggcattgtgccgcttctctgttagctgggagtttgtggcgtgtcgtttaactgaagtagagatgtgatgatgatctgaggtgagcgccattttttttttttggctaactctgtttgttctattcagtgaatatgagctctcttttttgtcccctttttatcattctgcttctatgggtcgcggagcgttcttggttggtgcagtgcaactgcaatgtcagtatgcatgccatagtaatgtgatggtgggatgtaatgctcctctgcttgctcgagctctttcttggttgtctatctatcttggatgatttgatcaatgtactacttattatctgctgatagtacccggggtccatttgcgctagtattgaacttcttcttctcttataatttgctgaaatcgcgagattatcgcttaggtttatcaatcagaacattatcatgtttatgcttcggttcgttcggtactggcacatgtcaaataagcggaaattctgctactttttcggcttaccatgaatggcacccaacatagcgtggttgttttgtcatgttgaactagtacaaatttttgcaccttaatatttgctttccgtgcatatccaattaacctgcccatcaaacttcttttcagatagctattattggggaaaaatattttcccagcttcttttatagtgactagactgtttatacatgcctgcgtaattgcttccttttgatgagctagtacattatagaccttgattttgttttgatggtacgggttggttattggtgtccatataatgaaattgcaacttgcgaccactccatgatagttacctggtcattccttgttggttaataagctattatttcaaatatgaatgttcatcctacttgtcatattatagacatttgtcacaattcaagtgaggaagaaattctgtaattttctatgggctagttgctgcgttaactacaataatggggctgacatcctgaggttatagtgttcgattaccaagttctaatttattaagaggaatgcaagtcaacatgcatgcctagcccatttacttttaatgagtagtgtaacttctgttgcagaagtactaatgacagctttctatataaatttcacaggttgatttatcaaggacaacaattcgtcagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaaggtgaggaaactactcagcaaaattatacagtcatcattttgtgcacacttttagttatcagcaacattcgtatgtaggacgaatatatgatttgggaggacaagttatagcagcaaacagtgcccctgttataactcaccttgcagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggtgagttgttctagaataaaatttaaatttgaggtaactggataataagcatctaattatttataatattctctctatttcttgcatgcgtgttattcaataattcttacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactggtgagcatattttaaattcagtttggctggcattgaagctatagtatttcagttgccttattagtatgtatgatcttttgttcagatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaaggtatatatgttaataatatttctcctgttgcaaggtcttcttttttctcgtgataacattggtaatttcctgcattgcagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaaggtgagcaagaaataatggcgtaatatatctcaattattaattccaccttcatcaaatttcttagagaaattgtgcatatatttttccatacttgcagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttc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/>
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001187049.1 RefSeq:Os04g0473900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 4]]<br />
[[Category:Chromosome 4]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os04g0473900&diff=171560Os04g04739002014-05-24T04:16:50Z<p>Daweih: Undo revision 171559 by Daweih (talk)</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
<br />
==Annotated Information==<br />
===Function===<br />
This gene is regarded as ''DWA1''.Putative ''DWA1'' plays important roles in drought resistance.The ''DWA1'' is composed of seven exons(Fig.1) The open reading fame (7,176 bp) encodes a protein consisting of 2,391 aa in ''japonica'' rice, Nipponbare, which is one of the 30 megaproteins (>2,300 aa) predicted in the rice genome<ref name="ref1"/>.<br />
<br />
===Mutation===<br />
[[File:图片3.png|right|thumb|200px|Fig.1 Identification of the drought-hypersensitive ''dwa1'' mutant in rice, in which functional complementation of ''dwa1'' restore the drought resistance(from reference<ref name="ref1" />)]]<br />
'''(1)'''There is an insertion of T-DNA in the sixth exon of ''DWA1'' (Fig.1A).<br><br />
'''(2)'''RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1B).<br><br />
'''(3)'''During the course of drought treatment, the mutant wilted and exhibited leaf rolling earlier than the WT.After severe drought treatment followed by rewatering, more than 90% of the WT plants survive, whereas the ''dwa1'' mutant plants are almost dead(Fig.1C and D).<br><br />
'''(4)'''Water loss rate of mutant is faster than WT(Fig.1E).<br><br />
'''(5)'''DWA1-functional complementation(DWA1-FC)lines which is inserted the full-length genomic fragment of ''DWA1'' into the ''dwa1'' mutant restore drought resistance(Fig.1F and G).<br><br />
'''(6)'''Plant height at the reproductive stage are also restored in the DWA1-FC lines(Fig.1H).<br />
<br />
===Expression===<br />
[[File:图片4.png|right|thumb|200px|Fig.2 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference<ref name="ref1" />)]]<br />
[[File:图片5.png|right|thumb|200px|Fig.3 Drought-induced expression of DWA1.(from reference<ref name="ref1" />)]]<br />
'''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.2A).<br><br />
'''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.2B 1-2), axillary bud (Fig.2B3), young panicle (Fig.2B4),spikelet (Fig.2B5), stem (Fig.2B6), young panicle (Fig.2B7), pistil (Fig.2B8), stamen (Fig.2B9), young embryo (Fig.2B10), SEM picture of rice leaf cuticle (Fig.2B11), cross-sections of stem (Fig.2B12) and leaf (Fig.2B13),and semithin sections of leaf (Fig.2B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.<br><br />
'''(3)'''What's more,after various phytohormones treatment,''DWA1'' expresses higher,such as abscisic acid, jasmonic acid, indole-3-acetic acid, and<br />
gibberellic acid (Fig.2C).<br><br />
'''(4)'''Under drought treatment,GUS staining shows that''DWA1'' is induced.For example,in Fig.3B,GUS staining in leaf before (1 and 3) and after (2 and 4) drought stress is shown.<br />
<br />
===Evolution===<br />
[[File:1(DWA1).png|right|thumb|200px|Fig.2 Unrooted phylogenetic tree and domain architecture of DWA1 protein orthologs in vascular plants and microbial relatives(from reference<ref name="ref1" />)]]<br />
At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region<ref name="ref1"/>(Fig.2).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi<ref name="ref2"/>,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain<ref name="ref1"/>.<br />
<br />
==Labs working on this gene==<br />
*National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan,China<br><br />
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<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os04g0473900|<br />
Description = Adrenodoxin reductase family protein|<br />
Version = NM_001187049.1 GI:297723228 GeneID:9266980|<br />
Length = 9882 bp|<br />
Definition = Oryza sativa Japonica Group Os04g0473900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|<br />
AP = Chromosome 4:24097480..24107361|<br />
CDS = 24097480..24100068,24100784..24104053,24104151..24104329,24104410..24104769,24104855..24105296<br>,24105409..24105542,24105689..24105892,24107127..24107361|<br />
GCID = <gbrowseImage1><br />
name=NC_008397:24097480..24107361<br />
source=RiceChromosome04<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008397:24097480..24107361<br />
source=RiceChromosome04<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactgatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttctggctcagaaaggcatctcaattccagattcttctgatcaggttggacttgttgtgattgcagaggttagagagggtaaggcagtctctaaagaggttgttaacaacatcaaagcacgtgtggtggaggagcatggtgtagctgttgcatctgtgaagcttatcaagcctagaaccatatgcaagacaacatctgggaagatacgaagatttgaatgcatgaggcagtttgttgacaatactcttagtttggctaagggcaaccatgtatctaagaaaaagggtctttttcggtctctgacaacaggaactggcatggagtcaaagaggtccctcttgaggcaaactgttgacctcaccattagccattggcccaaaagccaagttaaaaactccaatgaaataactgagtttctgacacaaatagtgtcagagcacactggcatttcaaaggataaaatttctctaactgacagtctaccttcgtatggttttgattctattgcagtggtacgagcagcccagaagctctcagattttcttggtgttcctgttggggccattgacatctttacagcaagctgcatttcggagcttgcaagcttcttggagaatttggtgcataagtcacaaacacagttggcaccttggcccaaaagcaaagtgaaaaactccaaggtgattatcgagtttctgacaaagattgtatcagatcaaacagggattccaaaggataaaatttctccgatggatagcctaccttcatatggttttgattcaattgcggtggtccaagcagcacagaaactctccgattttctcggtgtacctgttggagccattgacatatttacagctggttgcatttctgagctagctaccttcttggagaatttggcgcataagtcacagtcacagttggcacctggtgcatcttgctatattgaggatgagactcaagtggatcccatggatgccattagtccagaattcagtgttttgggtacgggcatcctgcaactgctggctcttacttatgtctgttttgtgctgctactcccagcatatcttgctagttcgacatacatgagcattttctctacagtcagtctggtcagatcaccattgctgagctatcttagttctttggttatggcacctattgtgtggatcttttatatctcgctcacttctttatcactgtctatcttggggaagtccttcctacagccaaattatgttctgatccctgatgtttcaatatggtcagttgactttgtgaaatggtgggcactaaacaaagcacaagcattagctgcaaagatgctagctgtgcatctaaagggcacaattttcttaaattattggttcaagatgcaaggagctcggattggttcatctgttgtcatcgacacagtagacataaccgatccatccctgttgacggttgcagatggtgcagttcttgcagaaggcgctcttgttcagggccatgaagtctgtaatgaagtgctgagttttaggcctatctggattggctgtgaggcttcgattggtccttatgcggtgcttcagaagggaactgttgtggaagatggagctgttgttccaccattgcagaagactggggctgggaaatcaacacgtcggactagcagaacttctgtaagcataaagaaagaagcagccaaagcaaacatgatacttgaacacctcgttagcatctacgcagttggcatcctgggtgctctgtccggtgcaatcgtctacacactatacactcacttgtccggcaaagcggcttcgcctctgcatttctcctttgcatgcattgctggtgcgttccactggttaccagctgccatcactgcctatgctgttattgtccaggagacacccacaagtgcactgtcatttgctctcttcactgccttcgccgacctgagctatggtgtcatcctcagtattctcaccagtataaccagcagagcccttgctgcaaagcctggaaccaagcagaatggcattgcgagtttgatccaccggcgcataaccatctctgctcatgtcaggtttgccaagatgctatctggaacagaagccttctgcgtgtacctgaggcttctcggagcaaagattggcaggcattgctctatccgagccatcaatccggtggcgaatcctgagctgatcagcgtcggtgatggcgtccatctaggtgacttctgcaatattgttccagggttctactccaaggggggattcaccagtgcagagattaaggtccaggagaacaccgtggttggcagtggcagcttgctcctccctggctgtgtcctccaggagaatgtcatcctcggcgcgctctccgtcgcgccggagaacgctgttctccggcgtggcggcgtctacgtcgggtcacagtctccggccatggtgaagaacaccttgcttgacgaggatgaacggatcgaagagatggatcaggcatacaagaagattgttggcaacctcgcggcgaacttggccatcaccaccatgaacgtcaagtcgaggtacttccaccgcatcggcgtgagcggccgcggcgtcctgaggatgtacgaggagataccgtcgtttccccggcacaagatcttcgcctccggcaagtccttcccggtgatcgtccgccacagcaacagcctgagcgccgacgacgacgcgaggctcgacgcccgcggcgccgcggtgcggatcctctcggacaacgacggcgaggcgcctctcctcgacctcacactcaagagcggcaaggcgttctacgcgcgcaccatcgccgacttcgccacctggctggtctgcggcctgccggcgagggaggagcaggtcaagcgctcgccgcacatccgcgacgccgtctggggctctctccggagcaccgactcgtacaccgtgctccactactactccaacatatgccggctgctgcggttcgacgacggcagggagatgtacgccaagttcaagctccgtcctgctgatccagacgtccccgaggactccggcaaggtggtgccgaggggcatcctgccgccggagaccggcgcgatccccagggacgaggatgacacgcgccctctgctcttcctcgccgacgacttccgccggagagtgggctcaccggacggggtgcgctacgtgttccagctgcagctcagggaggtgcccaccgacgccgccgcccgcgacgtcgccctcgactgcacccggccgtgggacgaggccgagttcccttacatcgacgtcggcgaggtcagcatcggccgcaacctcccgacggaggagacggagaagctcgagttcaacccgttcctccggtgccccgaggtggacgtcatcccggcgacgtcgtgcgcgcagagcgcgtccatcgaccacggccgctcgctggtctacgagatatgccagcgcctgcggaacggcgagccgctgcccgcgtcgtggcgagctttcctggagcagtcggacaccaagatcgacctgtcaggatgcccggtcgctgccgccgcgacacccacaaggtctaacgccggcgacgccacgaaggtgacgctggccaggacgtggtaccaggcgctctgggccacgctgtgccagccgctgctgcaaacgctggtgccgtactctgtcctgggcctcgtcatcttcctcccgctcaggggcctcctcgccgtcgccgcggccacccgcttcccgctctactggctcctcccggcgttctgggccgcgtcgggcgtcgccgccatggccacgtgcgcggcggcgaagtgggcgctggtcggcagcagggtcgacggcgacacggtgcacatctggtcgccggccgtgttcctggacacggtctggcaggccgtgcgcgccgccacggcggagtacttcgccgagctgacgcccgggtcggcgccgttcgcggcgtggatgcgggtgatgggcgcctccgtgtcccccggcgacggcgtgtacgtggactccatgggcgcgctgctgaacccggagatggtgcgcctggagcgcggcgccgccgtcgggcgggacgcgctgctgttcggccacgtgtacgagggcgaggccgggaaggtgaagttcggcgccgtgtccgtcggggaggacgggttcgtcggcagccgcgccgtggccatgcccagcgtcacggtggacgacggcggctgcctcgccgcgctcgggctggccatgaagggggagacggtgaagcacagcatgtaa</cdnaseq>|<br />
AA = <aaseq>MEERRATSPSRHSFCRDALGRRGGESGGGLVYIYRSPLRSPLQR HGLNREWRLERWRWRWRWEAATWRQRAAAHARIGEMDPKRSIDDQFSKLHPSLPVDTR IGIVGAGPSGLSAAYALAKLGYRNVTLFEKCHTVSGMCESIDIEEELGSDFEEMDTHK LSLIDSQTGNIRDLEVAEDYVSMVSLTLKLQDEANKSGRAGLHALSGLASDPTHEFLK QNGINSMPKSVAYGYTASGYGFVQDMPYAFIQEFTRTSMAGKIRRFKHGYMSMWERLS KSLPFEVFCDTQVLNVKRNSCGANVTIKNNNGEKQVLEFDKIILSGAVAFKNSKTYRS SSLTDGESEVVELNNLERELFSKVQTIDYYTTVVKINGFEHIPKGFYYFGEFMEDPTT IGHPVAMQRFFADTNIFLFWSYGNSADIKGSFVAKCVTDVVSSMGGNVQKVILQRRFK YFPHVSSKDMKDGFYERLESQLQGFQNTYYVGGLLAFELTERNASYSIMAVCKHFAID GEGELTPYVKRLFPLSHNRNPSPPRDLGELEGVEFPDLPSLDGYLQYWGTHKVTEKNV IYTWINEEGKLMNRRTYQELHGNASYIAQKLLTSTKPVIKPGDRVLLIHLPGLEFIDA FFGCIRAGVIPVPVLPPDPMQSGGQALLKVENISKMCNAVAILSTSSYHAAVRAGYIK NIVTLAKRVQKCSAQWPDIPWIHTDSWIKNYRRSSDSFNSDTVLFTKPQPSDLCFLQF TSGSTGDAKGVMITHEGLIHNVKTMKKRYRSTSKTVLVSWLPQYHDMGLIGGLFTALV SGGTSVLFSPMIFIRNPLLWLQTINDYHGTHSAGPNFAFELVIRRLEAEKNKVYDLSS MVFLMIAAEPVRQKTVRRFIELTQPFGLSEGVLAPGYGLAENCVYVTCAFGECKPVFI DWQGRVCCGYVEQDDTDTLIRIVDPDSLTEHQEDGVEGEIWISSPSSGVGYWGNSEMS QRTFFNQLKNHPNKKFTRTGDLGRTIDGNLFITGRIKDLIIVAGRNIYSADVEKTVES SSEVLRPGCCAVVGIPEEVLAQKGISIPDSSDQVGLVVIAEVREGKAVSKEVVNNIKA RVVEEHGVAVASVKLIKPRTICKTTSGKIRRFECMRQFVDNTLSLAKGNHVSKKKGLF RSLTTGTGMESKRSLLRQTVDLTISHWPKSQVKNSNEITEFLTQIVSEHTGISKDKIS LTDSLPSYGFDSIAVVRAAQKLSDFLGVPVGAIDIFTASCISELASFLENLVHKSQTQ LAPWPKSKVKNSKVIIEFLTKIVSDQTGIPKDKISPMDSLPSYGFDSIAVVQAAQKLS DFLGVPVGAIDIFTAGCISELATFLENLAHKSQSQLAPGASCYIEDETQVDPMDAISP EFSVLGTGILQLLALTYVCFVLLLPAYLASSTYMSIFSTVSLVRSPLLSYLSSLVMAP IVWIFYISLTSLSLSILGKSFLQPNYVLIPDVSIWSVDFVKWWALNKAQALAAKMLAV HLKGTIFLNYWFKMQGARIGSSVVIDTVDITDPSLLTVADGAVLAEGALVQGHEVCNE VLSFRPIWIGCEASIGPYAVLQKGTVVEDGAVVPPLQKTGAGKSTRRTSRTSVSIKKE AAKANMILEHLVSIYAVGILGALSGAIVYTLYTHLSGKAASPLHFSFACIAGAFHWLP AAITAYAVIVQETPTSALSFALFTAFADLSYGVILSILTSITSRALAAKPGTKQNGIA SLIHRRITISAHVRFAKMLSGTEAFCVYLRLLGAKIGRHCSIRAINPVANPELISVGD GVHLGDFCNIVPGFYSKGGFTSAEIKVQENTVVGSGSLLLPGCVLQENVILGALSVAP ENAVLRRGGVYVGSQSPAMVKNTLLDEDERIEEMDQAYKKIVGNLAANLAITTMNVKS RYFHRIGVSGRGVLRMYEEIPSFPRHKIFASGKSFPVIVRHSNSLSADDDARLDARGA AVRILSDNDGEAPLLDLTLKSGKAFYARTIADFATWLVCGLPAREEQVKRSPHIRDAV WGSLRSTDSYTVLHYYSNICRLLRFDDGREMYAKFKLRPADPDVPEDSGKVVPRGILP PETGAIPRDEDDTRPLLFLADDFRRRVGSPDGVRYVFQLQLREVPTDAAARDVALDCT RPWDEAEFPYIDVGEVSIGRNLPTEETEKLEFNPFLRCPEVDVIPATSCAQSASIDHG RSLVYEICQRLRNGEPLPASWRAFLEQSDTKIDLSGCPVAAAATPTRSNAGDATKVTL ARTWYQALWATLCQPLLQTLVPYSVLGLVIFLPLRGLLAVAAATRFPLYWLLPAFWAA SGVAAMATCAAAKWALVGSRVDGDTVHIWSPAVFLDTVWQAVRAATAEYFAELTPGSA PFAAWMRVMGASVSPGDGVYVDSMGALLNPEMVRLERGAAVGRDALLFGHVYEGEAGK VKFGAVSVGEDGFVGSRAVAMPSVTVDDGGCLAALGLAMKGETVKHSM</aaseq>|<br />
DNA = <dnaseqindica>7294..9882#3309..6578#3033..3211#2593..2952#2066..2507#1820..1953#1470..1673#1..235#atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggaggtatggattccccttccccactcgctgcgtgatgaaccgatgaaccgtagtacttgatgatatgcctgatttgcttcggcattgtgccgcttctctgttagctgggagtttgtggcgtgtcgtttaactgaagtagagatgtgatgatgatctgaggtgagcgccattttttttttttggctaactctgtttgttctattcagtgaatatgagctctcttttttgtcccctttttatcattctgcttctatgggtcgcggagcgttcttggttggtgcagtgcaactgcaatgtcagtatgcatgccatagtaatgtgatggtgggatgtaatgctcctctgcttgctcgagctctttcttggttgtctatctatcttggatgatttgatcaatgtactacttattatctgctgatagtacccggggtccatttgcgctagtattgaacttcttcttctcttataatttgctgaaatcgcgagattatcgcttaggtttatcaatcagaacattatcatgtttatgcttcggttcgttcggtactggcacatgtcaaataagcggaaattctgctactttttcggcttaccatgaatggcacccaacatagcgtggttgttttgtcatgttgaactagtacaaatttttgcaccttaatatttgctttccgtgcatatccaattaacctgcccatcaaacttcttttcagatagctattattggggaaaaatattttcccagcttcttttatagtgactagactgtttatacatgcctgcgtaattgcttccttttgatgagctagtacattatagaccttgattttgttttgatggtacgggttggttattggtgtccatataatgaaattgcaacttgcgaccactccatgatagttacctggtcattccttgttggttaataagctattatttcaaatatgaatgttcatcctacttgtcatattatagacatttgtcacaattcaagtgaggaagaaattctgtaattttctatgggctagttgctgcgttaactacaataatggggctgacatcctgaggttatagtgttcgattaccaagttctaatttattaagaggaatgcaagtcaacatgcatgcctagcccatttacttttaatgagtagtgtaacttctgttgcagaagtactaatgacagctttctatataaatttcacaggttgatttatcaaggacaacaattcgtcagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaaggtgaggaaactactcagcaaaattatacagtcatcattttgtgcacacttttagttatcagcaacattcgtatgtaggacgaatatatgatttgggaggacaagttatagcagcaaacagtgcccctgttataactcaccttgcagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggtgagttgttctagaataaaatttaaatttgaggtaactggataataagcatctaattatttataatattctctctatttcttgcatgcgtgttattcaataattcttacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactggtgagcatattttaaattcagtttggctggcattgaagctatagtatttcagttgccttattagtatgtatgatcttttgttcagatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaaggtatatatgttaataatatttctcctgttgcaaggtcttcttttttctcgtgataacattggtaatttcctgcattgcagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaaggtgagcaagaaataatggcgtaatatatctcaattattaattccaccttcatcaaatttcttagagaaattgtgcatatatttttccatacttgcagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttctggctcagaaaggcatctcaattccagattcttctgatcaggttggacttgttgtgattgcagaggttagagagggtaaggcagtctctaaagaggttgttaacaacatcaaagcacgtgtggtggaggagcatggtgtagctgttgcatctgtgaagcttatcaagcctagaaccatatgcaagacaacatctgggaagatacgaagatttgaatgcatgaggcagtttgttgacaatactcttagtttggctaagggcaaccatgtatctaagaaaaagggtctttttcggtctctgacaacaggaactggcatggagtcaaagaggtccctcttgaggcaaactgttgacctcaccattagccattggcccaaaagccaagttaaaaactccaatgaaataactgagtttctgacacaaatagtgtcagagcacactggcatttcaaaggataaaatttctctaactgacagtctaccttcgtatggttttgattctattgcagtggtacgagcagcccagaagctctcagattttcttggtgttcctgttggggccattgacatctttacagcaagctgcatttcggagcttgcaagcttcttggagaatttggtgcataagtcacaaacacagttggcaccttggcccaaaagcaaagtgaaaaactccaaggtgattatcgagtttctgacaaagattgtatcagatcaaacagggattccaaaggataaaatttctccgatggatagcctaccttcatatggttttgattcaattgcggtggtccaagcagcacagaaactctccgattttctcggtgtacctgttggagccattgacatatttacagctggttgcatttctgagctagctaccttcttggagaatttggcgcataagtcacagtcacagttggcacctggtgcatcttgctatattgaggatgagactcaagtggatcccatggatgccattagtccagaattcagtgttttgggtacgggcatcctgcaactgctggctcttacttatgtctgttttgtgctgctactcccagcatatcttgctagttcgacatacatgagcattttctctacagtcagtctggtcagatcaccattgctgagctatcttagttctttggttatggcacctattgtgtggatcttttatatctcgctcacttctttatcactgtctatcttggggaagtccttcctacagccaaattatgttctgatccctgatgtttcaatatggtcagttgactttgtgaaatggtgggcactaaacaaagcacaagcattagctgcaaagatgctagctgtgcatctaaagggcacaattttcttaaattattggttcaagatgcaaggagctcggattggttcatctgttgtcatcgacacagtagacataaccgatccatccctgttgacggttgcagatggtgcagttcttgcagaaggcgctcttgttcagggccatgaagtctgtaatgaagtgctgagttttaggcctatctggattggctgtgaggcttcgattggtccttatgcggtgcttcagaagggaactgttgtggaagatggagctgttgttccaccattgcagaagactggggctgggaaatcaacacgtcggactagcagaacttctgtaagcataaaggtaactaaagtatgtagacttcataatctacttcggtgtaataaaactatgatttgttatggtatacaacgatagtttgttccttcctgaccagaaacgtatagttgattatttctagacattgaatatagtaagctacagacttataattacaccataggccatttgaatttccttgtggtctttaacatggatgaagctgccaccaaagaacttccctacctttttacatttagtgaaaatattttttgagattcaggaagggacatatattttatcagggaaccataagcaaatcatttatgtacaagtggtcaagcagaaaaatcatataaattaaacaagatgaagggtttgtttggattgaagtcaatcttttggcaacttcaatagtattgtatagatatttggtccgataccaaatttttcccacactagtgcaaattctactcttaacaaatttggtagtgccgaaatttaccaatgttttggcacttccagtattttggtaagacaaaaaaatggcttcaaaccaaactagcccaaactcttttctgcatggtgcatagtgtctgcgtgtgtgcattttcattcgacagtggggtatatatctaactgccttcaaagttctcgattgcttctgcagaacatgtttgttcatggccatatttatataatgttattcgaatacaaaccgagtattttttctttgcagaaagaagcagccaaagcaaacatgatacttgaacacctcgttagcatctacgcagttggcatcctgggtgctctgtccggtgcaatcgtctacacactatacactcacttgtccggcaaagcggcttcgcctctgcatttctcctttgcatgcattgctggtgcgttccactggttaccagctgccatcactgcctatgctgttattgtccaggagacacccacaagtgcactgtcatttgctctcttcactgccttcgccgacctgagctatggtgtcatcctcagtattctcaccagtataaccagcagagcccttgctgcaaagcctggaaccaagcagaatggcattgcgagtttgatccaccggcgcataaccatctctgctcatgtcaggtttgccaagatgctatctggaacagaagccttctgcgtgtacctgaggcttctcggagcaaagattggcaggcattgctctatccgagccatcaatccggtggcgaatcctgagctgatcagcgtcggtgatggcgtccatctaggtgacttctgcaatattgttccagggttctactccaaggggggattcaccagtgcagagattaaggtccaggagaacaccgtggttggcagtggcagcttgctcctccctggctgtgtcctccaggagaatgtcatcctcggcgcgctctccgtcgcgccggagaacgctgttctccggcgtggcggcgtctacgtcgggtcacagtctccggccatggtgaagaacaccttgcttgacgaggatgaacggatcgaagagatggatcaggcatacaagaagattgttggcaacctcgcggcgaacttggccatcaccaccatgaacgtcaagtcgaggtacttccaccgcatcggcgtgagcggccgcggcgtcctgaggatgtacgaggagataccgtcgtttccccggcacaagatcttcgcctccggcaagtccttcccggtgatcgtccgccacagcaacagcctgagcgccgacgacgacgcgaggctcgacgcccgcggcgccgcggtgcggatcctctcggacaacgacggcgaggcgcctctcctcgacctcacactcaagagcggcaaggcgttctacgcgcgcaccatcgccgacttcgccacctggctggtctgcggcctgccggcgagggaggagcaggtcaagcgctcgccgcacatccgcgacgccgtctggggctctctccggagcaccgactcgtacaccgtgctccactactactccaacatatgccggctgctgcggttcgacgacggcagggagatgtacgccaagttcaagctccgtcctgctgatccagacgtccccgaggactccggcaaggtggtgccgaggggcatcctgccgccggagaccggcgcgatccccagggacgaggatgacacgcgccctctgctcttcctcgccgacgacttccgccggagagtgggctcaccggacggggtgcgctacgtgttccagctgcagctcagggaggtgcccaccgacgccgccgcccgcgacgtcgccctcgactgcacccggccgtgggacgaggccgagttcccttacatcgacgtcggcgaggtcagcatcggccgcaacctcccgacggaggagacggagaagctcgagttcaacccgttcctccggtgccccgaggtggacgtcatcccggcgacgtcgtgcgcgcagagcgcgtccatcgaccacggccgctcgctggtctacgagatatgccagcgcctgcggaacggcgagccgctgcccgcgtcgtggcgagctttcctggagcagtcggacaccaagatcgacctgtcaggatgcccggtcgctgccgccgcgacacccacaaggtctaacgccggcgacgccacgaaggtgacgctggccaggacgtggtaccaggcgctctgggccacgctgtgccagccgctgctgcaaacgctggtgccgtactctgtcctgggcctcgtcatcttcctcccgctcaggggcctcctcgccgtcgccgcggccacccgcttcccgctctactggctcctcccggcgttctgggccgcgtcgggcgtcgccgccatggccacgtgcgcggcggcgaagtgggcgctggtcggcagcagggtcgacggcgacacggtgcacatctggtcgccggccgtgttcctggacacggtctggcaggccgtgcgcgccgccacggcggagtacttcgccgagctgacgcccgggtcggcgccgttcgcggcgtggatgcgggtgatgggcgcctccgtgtcccccggcgacggcgtgtacgtggactccatgggcgcgctgctgaacccggagatggtgcgcctggagcgcggcgccgccgtcgggcgggacgcgctgctgttcggccacgtgtacgagggcgaggccgggaaggtgaagttcggcgccgtgtccgtcggggaggacgggttcgtcggcagccgcgccgtggccatgcccagcgtcacggtggacgacggcggctgcctcgccgcgctcgggctggccatgaagggggagacggtgaagcacagcatgtaa</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001187049.1 RefSeq:Os04g0473900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 4]]<br />
[[Category:Chromosome 4]]<br />
<br />
==References==<br />
<references><br />
<ref name="ref1">Xiaoyi Zhu, Lizhong Xiong.（2013）Putative megaenzyme DWA1 plays essential roles in drought resistance by regulating stress-induced wax<br />
deposition in rice.PANS 110(44): 17790-17795.</ref><br />
<ref name="ref2">Marahiel MA, Stachelhaus T, Mootz HD.(1997) Modular peptide synthetases involved in nonribosomal peptide synthesis.Chem Rev 97(7):2651–2674.</ref></div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os04g0473900&diff=171559Os04g04739002014-05-24T04:16:04Z<p>Daweih: </p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
<br />
==Annotated Information==<br />
===Function===<br />
This gene is regarded as ''DWA1''.Putative ''DWA1'' plays important roles in drought resistance.The ''DWA1'' is composed of seven exons(Fig.1) The open reading fame (7,176 bp) encodes a protein consisting of 2,391 aa in ''japonica'' rice, Nipponbare, which is one of the 30 megaproteins (>2,300 aa) predicted in the rice genome<ref name="ref1"/>.<br />
<br />
===Mutation===<br />
[[File:图片3.png|right|thumb|200px|Fig.1 Identification of the drought-hypersensitive ''dwa1'' mutant in rice, in which functional complementation of ''dwa1'' restore the drought resistance(from reference<ref name="ref1" />)]]<br />
'''(1)'''There is an insertion of T-DNA in the sixth exon of ''DWA1'' (Fig.1A).<br><br />
'''(2)'''RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1B).<br><br />
'''(3)'''During the course of drought treatment, the mutant wilted and exhibited leaf rolling earlier than the WT.After severe drought treatment followed by rewatering, more than 90% of the WT plants survive, whereas the ''dwa1'' mutant plants are almost dead(Fig.1C and D).<br><br />
'''(4)'''Water loss rate of mutant is faster than WT(Fig.1E).<br><br />
'''(5)'''DWA1-functional complementation(DWA1-FC)lines which is inserted the full-length genomic fragment of ''DWA1'' into the ''dwa1'' mutant restore drought resistance(Fig.1F and G).<br><br />
'''(6)'''Plant height at the reproductive stage are also restored in the DWA1-FC lines(Fig.1H).<br />
<br />
===Expression===<br />
[[File:图片4.png|right|thumb|200px|Fig.2 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference<ref name="ref1" />)]]<br />
[[File:图片5.png|right|thumb|200px|Fig.3 Drought-induced expression of DWA1.(from reference<ref name="ref1" />)]]<br />
'''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.2A).<br><br />
'''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.2B 1-2), axillary bud (Fig.2B3), young panicle (Fig.2B4),spikelet (Fig.2B5), stem (Fig.2B6), young panicle (Fig.2B7), pistil (Fig.2B8), stamen (Fig.2B9), young embryo (Fig.2B10), SEM picture of rice leaf cuticle (Fig.2B11), cross-sections of stem (Fig.2B12) and leaf (Fig.2B13),and semithin sections of leaf (Fig.2B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.<br><br />
'''(3)'''What's more,after various phytohormones treatment,''DWA1'' expresses higher,such as abscisic acid, jasmonic acid, indole-3-acetic acid, and<br />
gibberellic acid (Fig.2C).<br><br />
'''(4)'''Under drought treatment,GUS staining shows that''DWA1'' is induced.For example,in Fig.3B,GUS staining in leaf before (1 and 3) and after (2 and 4) drought stress is shown.<br />
<br />
===Evolution===<br />
[[File:1(DWA1).png|right|thumb|200px|Fig.2 Unrooted phylogenetic tree and domain architecture of DWA1 protein orthologs in vascular plants and microbial relatives(from reference<ref name="ref1" />)]]<br />
At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region<ref name="ref1"/>(Fig.2).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi<ref name="ref2"/>,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain<ref name="ref1"/>.<br />
<br />
==Labs working on this gene==<br />
*National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan,China<br><br />
<br />
<br />
==References==<br />
<references><br />
<ref name="ref1">Xiaoyi Zhu, Lizhong Xiong.（2013）Putative megaenzyme DWA1 plays essential roles in drought resistance by regulating stress-induced wax<br />
deposition in rice.PANS 110(44): 17790-17795.</ref><br />
<ref name="ref2">Marahiel MA, Stachelhaus T, Mootz HD.(1997) Modular peptide synthetases involved in nonribosomal peptide synthesis.Chem Rev 97(7):2651–2674.</ref><br />
<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os04g0473900|<br />
Description = Adrenodoxin reductase family protein|<br />
Version = NM_001187049.1 GI:297723228 GeneID:9266980|<br />
Length = 9882 bp|<br />
Definition = Oryza sativa Japonica Group Os04g0473900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|<br />
AP = Chromosome 4:24097480..24107361|<br />
CDS = 24097480..24100068,24100784..24104053,24104151..24104329,24104410..24104769,24104855..24105296<br>,24105409..24105542,24105689..24105892,24107127..24107361|<br />
GCID = <gbrowseImage1><br />
name=NC_008397:24097480..24107361<br />
source=RiceChromosome04<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008397:24097480..24107361<br />
source=RiceChromosome04<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactgatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttctggctcagaaaggcatctcaattccagattcttctgatcaggttggacttgttgtgattgcagaggttagagagggtaaggcagtctctaaagaggttgttaacaacatcaaagcacgtgtggtggaggagcatggtgtagctgttgcatctgtgaagcttatcaagcctagaaccatatgcaagacaacatctgggaagatacgaagatttgaatgcatgaggcagtttgttgacaatactcttagtttggctaagggcaaccatgtatctaagaaaaagggtctttttcggtctctgacaacaggaactggcatggagtcaaagaggtccctcttgaggcaaactgttgacctcaccattagccattggcccaaaagccaagttaaaaactccaatgaaataactgagtttctgacacaaatagtgtcagagcacactggcatttcaaaggataaaatttctctaactgacagtctaccttcgtatggttttgattctattgcagtggtacgagcagcccagaagctctcagattttcttggtgttcctgttggggccattgacatctttacagcaagctgcatttcggagcttgcaagcttcttggagaatttggtgcataagtcacaaacacagttggcaccttggcccaaaagcaaagtgaaaaactccaaggtgattatcgagtttctgacaaagattgtatcagatcaaacagggattccaaaggataaaatttctccgatggatagcctaccttcatatggttttgattcaattgcggtggtccaagcagcacagaaactctccgattttctcggtgtacctgttggagccattgacatatttacagctggttgcatttctgagctagctaccttcttggagaatttggcgcataagtcacagtcacagttggcacctggtgcatcttgctatattgaggatgagactcaagtggatcccatggatgccattagtccagaattcagtgttttgggtacgggcatcctgcaactgctggctcttacttatgtctgttttgtgctgctactcccagcatatcttgctagttcgacatacatgagcattttctctacagtcagtctggtcagatcaccattgctgagctatcttagttctttggttatggcacctattgtgtggatcttttatatctcgctcacttctttatcactgtctatcttggggaagtccttcctacagccaaattatgttctgatccctgatgtttcaatatggtcagttgactttgtgaaatggtgggcactaaacaaagcacaagcattagctgcaaagatgctagctgtgcatctaaagggcacaattttcttaaattattggttcaagatgcaaggagctcggattggttcatctgttgtcatcgacacagtagacataaccgatccatccctgttgacggttgcagatggtgcagttcttgcagaaggcgctcttgttcagggccatgaagtctgtaatgaagtgctgagttttaggcctatctggattggctgtgaggcttcgattggtccttatgcggtgcttcagaagggaactgttgtggaagatggagctgttgttccaccattgcagaagactggggctgggaaatcaacacgtcggactagcagaacttctgtaagcataaagaaagaagcagccaaagcaaacatgatacttgaacacctcgttagcatctacgcagttggcatcctgggtgctctgtccggtgcaatcgtctacacactatacactcacttgtccggcaaagcggcttcgcctctgcatttctcctttgcatgcattgctggtgcgttccactggttaccagctgccatcactgcctatgctgttattgtccaggagacacccacaagtgcactgtcatttgctctcttcactgccttcgccgacctgagctatggtgtcatcctcagtattctcaccagtataaccagcagagcccttgctgcaaagcctggaaccaagcagaatggcattgcgagtttgatccaccggcgcataaccatctctgctcatgtcaggtttgccaagatgctatctggaacagaagccttctgcgtgtacctgaggcttctcggagcaaagattggcaggcattgctctatccgagccatcaatccggtggcgaatcctgagctgatcagcgtcggtgatggcgtccatctaggtgacttctgcaatattgttccagggttctactccaaggggggattcaccagtgcagagattaaggtccaggagaacaccgtggttggcagtggcagcttgctcctccctggctgtgtcctccaggagaatgtcatcctcggcgcgctctccgtcgcgccggagaacgctgttctccggcgtggcggcgtctacgtcgggtcacagtctccggccatggtgaagaacaccttgcttgacgaggatgaacggatcgaagagatggatcaggcatacaagaagattgttggcaacctcgcggcgaacttggccatcaccaccatgaacgtcaagtcgaggtacttccaccgcatcggcgtgagcggccgcggcgtcctgaggatgtacgaggagataccgtcgtttccccggcacaagatcttcgcctccggcaagtccttcccggtgatcgtccgccacagcaacagcctgagcgccgacgacgacgcgaggctcgacgcccgcggcgccgcggtgcggatcctctcggacaacgacggcgaggcgcctctcctcgacctcacactcaagagcggcaaggcgttctacgcgcgcaccatcgccgacttcgccacctggctggtctgcggcctgccggcgagggaggagcaggtcaagcgctcgccgcacatccgcgacgccgtctggggctctctccggagcaccgactcgtacaccgtgctccactactactccaacatatgccggctgctgcggttcgacgacggcagggagatgtacgccaagttcaagctccgtcctgctgatccagacgtccccgaggactccggcaaggtggtgccgaggggcatcctgccgccggagaccggcgcgatccccagggacgaggatgacacgcgccctctgctcttcctcgccgacgacttccgccggagagtgggctcaccggacggggtgcgctacgtgttccagctgcagctcagggaggtgcccaccgacgccgccgcccgcgacgtcgccctcgactgcacccggccgtgggacgaggccgagttcccttacatcgacgtcggcgaggtcagcatcggccgcaacctcccgacggaggagacggagaagctcgagttcaacccgttcctccggtgccccgaggtggacgtcatcccggcgacgtcgtgcgcgcagagcgcgtccatcgaccacggccgctcgctggtctacgagatatgccagcgcctgcggaacggcgagccgctgcccgcgtcgtggcgagctttcctggagcagtcggacaccaagatcgacctgtcaggatgcccggtcgctgccgccgcgacacccacaaggtctaacgccggcgacgccacgaaggtgacgctggccaggacgtggtaccaggcgctctgggccacgctgtgccagccgctgctgcaaacgctggtgccgtactctgtcctgggcctcgtcatcttcctcccgctcaggggcctcctcgccgtcgccgcggccacccgcttcccgctctactggctcctcccggcgttctgggccgcgtcgggcgtcgccgccatggccacgtgcgcggcggcgaagtgggcgctggtcggcagcagggtcgacggcgacacggtgcacatctggtcgccggccgtgttcctggacacggtctggcaggccgtgcgcgccgccacggcggagtacttcgccgagctgacgcccgggtcggcgccgttcgcggcgtggatgcgggtgatgggcgcctccgtgtcccccggcgacggcgtgtacgtggactccatgggcgcgctgctgaacccggagatggtgcgcctggagcgcggcgccgccgtcgggcgggacgcgctgctgttcggccacgtgtacgagggcgaggccgggaaggtgaagttcggcgccgtgtccgtcggggaggacgggttcgtcggcagccgcgccgtggccatgcccagcgtcacggtggacgacggcggctgcctcgccgcgctcgggctggccatgaagggggagacggtgaagcacagcatgtaa</cdnaseq>|<br />
AA = <aaseq>MEERRATSPSRHSFCRDALGRRGGESGGGLVYIYRSPLRSPLQR HGLNREWRLERWRWRWRWEAATWRQRAAAHARIGEMDPKRSIDDQFSKLHPSLPVDTR IGIVGAGPSGLSAAYALAKLGYRNVTLFEKCHTVSGMCESIDIEEELGSDFEEMDTHK LSLIDSQTGNIRDLEVAEDYVSMVSLTLKLQDEANKSGRAGLHALSGLASDPTHEFLK QNGINSMPKSVAYGYTASGYGFVQDMPYAFIQEFTRTSMAGKIRRFKHGYMSMWERLS KSLPFEVFCDTQVLNVKRNSCGANVTIKNNNGEKQVLEFDKIILSGAVAFKNSKTYRS SSLTDGESEVVELNNLERELFSKVQTIDYYTTVVKINGFEHIPKGFYYFGEFMEDPTT IGHPVAMQRFFADTNIFLFWSYGNSADIKGSFVAKCVTDVVSSMGGNVQKVILQRRFK YFPHVSSKDMKDGFYERLESQLQGFQNTYYVGGLLAFELTERNASYSIMAVCKHFAID GEGELTPYVKRLFPLSHNRNPSPPRDLGELEGVEFPDLPSLDGYLQYWGTHKVTEKNV IYTWINEEGKLMNRRTYQELHGNASYIAQKLLTSTKPVIKPGDRVLLIHLPGLEFIDA FFGCIRAGVIPVPVLPPDPMQSGGQALLKVENISKMCNAVAILSTSSYHAAVRAGYIK NIVTLAKRVQKCSAQWPDIPWIHTDSWIKNYRRSSDSFNSDTVLFTKPQPSDLCFLQF TSGSTGDAKGVMITHEGLIHNVKTMKKRYRSTSKTVLVSWLPQYHDMGLIGGLFTALV SGGTSVLFSPMIFIRNPLLWLQTINDYHGTHSAGPNFAFELVIRRLEAEKNKVYDLSS MVFLMIAAEPVRQKTVRRFIELTQPFGLSEGVLAPGYGLAENCVYVTCAFGECKPVFI DWQGRVCCGYVEQDDTDTLIRIVDPDSLTEHQEDGVEGEIWISSPSSGVGYWGNSEMS QRTFFNQLKNHPNKKFTRTGDLGRTIDGNLFITGRIKDLIIVAGRNIYSADVEKTVES SSEVLRPGCCAVVGIPEEVLAQKGISIPDSSDQVGLVVIAEVREGKAVSKEVVNNIKA RVVEEHGVAVASVKLIKPRTICKTTSGKIRRFECMRQFVDNTLSLAKGNHVSKKKGLF RSLTTGTGMESKRSLLRQTVDLTISHWPKSQVKNSNEITEFLTQIVSEHTGISKDKIS LTDSLPSYGFDSIAVVRAAQKLSDFLGVPVGAIDIFTASCISELASFLENLVHKSQTQ LAPWPKSKVKNSKVIIEFLTKIVSDQTGIPKDKISPMDSLPSYGFDSIAVVQAAQKLS DFLGVPVGAIDIFTAGCISELATFLENLAHKSQSQLAPGASCYIEDETQVDPMDAISP EFSVLGTGILQLLALTYVCFVLLLPAYLASSTYMSIFSTVSLVRSPLLSYLSSLVMAP IVWIFYISLTSLSLSILGKSFLQPNYVLIPDVSIWSVDFVKWWALNKAQALAAKMLAV HLKGTIFLNYWFKMQGARIGSSVVIDTVDITDPSLLTVADGAVLAEGALVQGHEVCNE VLSFRPIWIGCEASIGPYAVLQKGTVVEDGAVVPPLQKTGAGKSTRRTSRTSVSIKKE AAKANMILEHLVSIYAVGILGALSGAIVYTLYTHLSGKAASPLHFSFACIAGAFHWLP AAITAYAVIVQETPTSALSFALFTAFADLSYGVILSILTSITSRALAAKPGTKQNGIA SLIHRRITISAHVRFAKMLSGTEAFCVYLRLLGAKIGRHCSIRAINPVANPELISVGD GVHLGDFCNIVPGFYSKGGFTSAEIKVQENTVVGSGSLLLPGCVLQENVILGALSVAP ENAVLRRGGVYVGSQSPAMVKNTLLDEDERIEEMDQAYKKIVGNLAANLAITTMNVKS RYFHRIGVSGRGVLRMYEEIPSFPRHKIFASGKSFPVIVRHSNSLSADDDARLDARGA AVRILSDNDGEAPLLDLTLKSGKAFYARTIADFATWLVCGLPAREEQVKRSPHIRDAV WGSLRSTDSYTVLHYYSNICRLLRFDDGREMYAKFKLRPADPDVPEDSGKVVPRGILP PETGAIPRDEDDTRPLLFLADDFRRRVGSPDGVRYVFQLQLREVPTDAAARDVALDCT RPWDEAEFPYIDVGEVSIGRNLPTEETEKLEFNPFLRCPEVDVIPATSCAQSASIDHG RSLVYEICQRLRNGEPLPASWRAFLEQSDTKIDLSGCPVAAAATPTRSNAGDATKVTL ARTWYQALWATLCQPLLQTLVPYSVLGLVIFLPLRGLLAVAAATRFPLYWLLPAFWAA SGVAAMATCAAAKWALVGSRVDGDTVHIWSPAVFLDTVWQAVRAATAEYFAELTPGSA PFAAWMRVMGASVSPGDGVYVDSMGALLNPEMVRLERGAAVGRDALLFGHVYEGEAGK VKFGAVSVGEDGFVGSRAVAMPSVTVDDGGCLAALGLAMKGETVKHSM</aaseq>|<br />
DNA = <dnaseqindica>7294..9882#3309..6578#3033..3211#2593..2952#2066..2507#1820..1953#1470..1673#1..235#atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggaggtatggattccccttccccactcgctgcgtgatgaaccgatgaaccgtagtacttgatgatatgcctgatttgcttcggcattgtgccgcttctctgttagctgggagtttgtggcgtgtcgtttaactgaagtagagatgtgatgatgatctgaggtgagcgccattttttttttttggctaactctgtttgttctattcagtgaatatgagctctcttttttgtcccctttttatcattctgcttctatgggtcgcggagcgttcttggttggtgcagtgcaactgcaatgtcagtatgcatgccatagtaatgtgatggtgggatgtaatgctcctctgcttgctcgagctctttcttggttgtctatctatcttggatgatttgatcaatgtactacttattatctgctgatagtacccggggtccatttgcgctagtattgaacttcttcttctcttataatttgctgaaatcgcgagattatcgcttaggtttatcaatcagaacattatcatgtttatgcttcggttcgttcggtactggcacatgtcaaataagcggaaattctgctactttttcggcttaccatgaatggcacccaacatagcgtggttgttttgtcatgttgaactagtacaaatttttgcaccttaatatttgctttccgtgcatatccaattaacctgcccatcaaacttcttttcagatagctattattggggaaaaatattttcccagcttcttttatagtgactagactgtttatacatgcctgcgtaattgcttccttttgatgagctagtacattatagaccttgattttgttttgatggtacgggttggttattggtgtccatataatgaaattgcaacttgcgaccactccatgatagttacctggtcattccttgttggttaataagctattatttcaaatatgaatgttcatcctacttgtcatattatagacatttgtcacaattcaagtgaggaagaaattctgtaattttctatgggctagttgctgcgttaactacaataatggggctgacatcctgaggttatagtgttcgattaccaagttctaatttattaagaggaatgcaagtcaacatgcatgcctagcccatttacttttaatgagtagtgtaacttctgttgcagaagtactaatgacagctttctatataaatttcacaggttgatttatcaaggacaacaattcgtcagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaaggtgaggaaactactcagcaaaattatacagtcatcattttgtgcacacttttagttatcagcaacattcgtatgtaggacgaatatatgatttgggaggacaagttatagcagcaaacagtgcccctgttataactcaccttgcagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggtgagttgttctagaataaaatttaaatttgaggtaactggataataagcatctaattatttataatattctctctatttcttgcatgcgtgttattcaataattcttacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactggtgagcatattttaaattcagtttggctggcattgaagctatagtatttcagttgccttattagtatgtatgatcttttgttcagatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaaggtatatatgttaataatatttctcctgttgcaaggtcttcttttttctcgtgataacattggtaatttcctgcattgcagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaaggtgagcaagaaataatggcgtaatatatctcaattattaattccaccttcatcaaatttcttagagaaattgtgcatatatttttccatacttgcagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttc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/>
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001187049.1 RefSeq:Os04g0473900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 4]]<br />
[[Category:Chromosome 4]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os04g0473900&diff=171557Os04g04739002014-05-24T04:04:41Z<p>Daweih: </p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
<br />
==Annotated Information==<br />
===Function===<br />
This gene is regarded as ''DWA1''.Putative ''DWA1'' plays important roles in drought resistance.The ''DWA1'' is composed of seven exons(Fig.1) The open reading fame (7,176 bp) encodes a protein consisting of 2,391 aa in ''japonica'' rice, Nipponbare, which is one of the 30 megaproteins (>2,300 aa) predicted in the rice genome<ref name="ref1"/>.<br />
<br />
===Mutation===<br />
[[File:图片3.png|right|thumb|200px|Fig.1 Identification of the drought-hypersensitive ''dwa1'' mutant in rice, in which functional complementation of ''dwa1'' restore the drought resistance(from reference<ref name="ref1" />)]]<br />
'''(1)'''There is an insertion of T-DNA in the sixth exon of ''DWA1'' (Fig.1A).<br><br />
'''(2)'''RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1B).<br><br />
'''(3)'''During the course of drought treatment, the mutant wilted and exhibited leaf rolling earlier than the WT.After severe drought treatment followed by rewatering, more than 90% of the WT plants survive, whereas the ''dwa1'' mutant plants are almost dead(Fig.1C and D).<br><br />
'''(4)'''Water loss rate of mutant is faster than WT(Fig.1E).<br><br />
'''(5)'''DWA1-functional complementation(DWA1-FC)lines which is inserted the full-length genomic fragment of ''DWA1'' into the ''dwa1'' mutant restore drought resistance(Fig.1F and G).<br><br />
'''(6)'''Plant height at the reproductive stage are also restored in the DWA1-FC lines(Fig.1H).<br />
<br />
===Expression===<br />
[[File:图片4.png|right|thumb|200px|Fig.2 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference<ref name="ref1" />)]]<br />
[[File:图片5.png|right|thumb|200px|Fig.3 Drought-induced expression of DWA1.(from reference<ref name="ref1" />)]]<br />
'''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.2A).<br><br />
'''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.2B 1-2), axillary bud (Fig.2B3), young panicle (Fig.2B4),spikelet (Fig.2B5), stem (Fig.2B6), young panicle (Fig.2B7), pistil (Fig.2B8), stamen (Fig.2B9), young embryo (Fig.2B10), SEM picture of rice leaf cuticle (Fig.2B11), cross-sections of stem (Fig.2B12) and leaf (Fig.2B13),and semithin sections of leaf (Fig.2B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.<br><br />
'''(3)'''What's more,after various phytohormones treatment,''DWA1'' expresses higher,such as abscisic acid, jasmonic acid, indole-3-acetic acid, and<br />
gibberellic acid (Fig.2C).<br><br />
'''(4)'''Under drought treatment,GUS staining shows that''DWA1'' is induced.For example,in Fig.3B,GUS staining in leaf before (1 and 3) and after (2 and 4) drought stress is shown.<br />
<br />
===Evolution===<br />
[[File:1(DWA1).png|right|thumb|200px|Fig.2 Unrooted phylogenetic tree and domain architecture of DWA1 protein orthologs in vascular plants and microbial relatives(from reference<ref name="ref1" />)]]<br />
At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region<ref name="ref1"/>(Fig.2).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi<ref name="ref2"/>,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain<ref name="ref1"/>.<br />
<br />
==Labs working on this gene==<br />
*National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan,China<br><br />
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<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os04g0473900|<br />
Description = Adrenodoxin reductase family protein|<br />
Version = NM_001187049.1 GI:297723228 GeneID:9266980|<br />
Length = 9882 bp|<br />
Definition = Oryza sativa Japonica Group Os04g0473900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|<br />
AP = Chromosome 4:24097480..24107361|<br />
CDS = 24097480..24100068,24100784..24104053,24104151..24104329,24104410..24104769,24104855..24105296<br>,24105409..24105542,24105689..24105892,24107127..24107361|<br />
GCID = <gbrowseImage1><br />
name=NC_008397:24097480..24107361<br />
source=RiceChromosome04<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008397:24097480..24107361<br />
source=RiceChromosome04<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactgatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttctggctcagaaaggcatctcaattccagattcttctgatcaggttggacttgttgtgattgcagaggttagagagggtaaggcagtctctaaagaggttgttaacaacatcaaagcacgtgtggtggaggagcatggtgtagctgttgcatctgtgaagcttatcaagcctagaaccatatgcaagacaacatctgggaagatacgaagatttgaatgcatgaggcagtttgttgacaatactcttagtttggctaagggcaaccatgtatctaagaaaaagggtctttttcggtctctgacaacaggaactggcatggagtcaaagaggtccctcttgaggcaaactgttgacctcaccattagccattggcccaaaagccaagttaaaaactccaatgaaataactgagtttctgacacaaatagtgtcagagcacactggcatttcaaaggataaaatttctctaactgacagtctaccttcgtatggttttgattctattgcagtggtacgagcagcccagaagctctcagattttcttggtgttcctgttggggccattgacatctttacagcaagctgcatttcggagcttgcaagcttcttggagaatttggtgcataagtcacaaacacagttggcaccttggcccaaaagcaaagtgaaaaactccaaggtgattatcgagtttctgacaaagattgtatcagatcaaacagggattccaaaggataaaatttctccgatggatagcctaccttcatatggttttgattcaattgcggtggtccaagcagcacagaaactctccgattttctcggtgtacctgttggagccattgacatatttacagctggttgcatttctgagctagctaccttcttggagaatttggcgcataagtcacagtcacagttggcacctggtgcatcttgctatattgaggatgagactcaagtggatcccatggatgccattagtccagaattcagtgttttgggtacgggcatcctgcaactgctggctcttacttatgtctgttttgtgctgctactcccagcatatcttgctagttcgacatacatgagcattttctctacagtcagtctggtcagatcaccattgctgagctatcttagttctttggttatggcacctattgtgtggatcttttatatctcgctcacttctttatcactgtctatcttggggaagtccttcctacagccaaattatgttctgatccctgatgtttcaatatggtcagttgactttgtgaaatggtgggcactaaacaaagcacaagcattagctgcaaagatgctagctgtgcatctaaagggcacaattttcttaaattattggttcaagatgcaaggagctcggattggttcatctgttgtcatcgacacagtagacataaccgatccatccctgttgacggttgcagatggtgcagttcttgcagaaggcgctcttgttcagggccatgaagtctgtaatgaagtgctgagttttaggcctatctggattggctgtgaggcttcgattggtccttatgcggtgcttcagaagggaactgttgtggaagatggagctgttgttccaccattgcagaagactggggctgggaaatcaacacgtcggactagcagaacttctgtaagcataaagaaagaagcagccaaagcaaacatgatacttgaacacctcgttagcatctacgcagttggcatcctgggtgctctgtccggtgcaatcgtctacacactatacactcacttgtccggcaaagcggcttcgcctctgcatttctcctttgcatgcattgctggtgcgttccactggttaccagctgccatcactgcctatgctgttattgtccaggagacacccacaagtgcactgtcatttgctctcttcactgccttcgccgacctgagctatggtgtcatcctcagtattctcaccagtataaccagcagagcccttgctgcaaagcctggaaccaagcagaatggcattgcgagtttgatccaccggcgcataaccatctctgctcatgtcaggtttgccaagatgctatctggaacagaagccttctgcgtgtacctgaggcttctcggagcaaagattggcaggcattgctctatccgagccatcaatccggtggcgaatcctgagctgatcagcgtcggtgatggcgtccatctaggtgacttctgcaatattgttccagggttctactccaaggggggattcaccagtgcagagattaaggtccaggagaacaccgtggttggcagtggcagcttgctcctccctggctgtgtcctccaggagaatgtcatcctcggcgcgctctccgtcgcgccggagaacgctgttctccggcgtggcggcgtctacgtcgggtcacagtctccggccatggtgaagaacaccttgcttgacgaggatgaacggatcgaagagatggatcaggcatacaagaagattgttggcaacctcgcggcgaacttggccatcaccaccatgaacgtcaagtcgaggtacttccaccgcatcggcgtgagcggccgcggcgtcctgaggatgtacgaggagataccgtcgtttccccggcacaagatcttcgcctccggcaagtccttcccggtgatcgtccgccacagcaacagcctgagcgccgacgacgacgcgaggctcgacgcccgcggcgccgcggtgcggatcctctcggacaacgacggcgaggcgcctctcctcgacctcacactcaagagcggcaaggcgttctacgcgcgcaccatcgccgacttcgccacctggctggtctgcggcctgccggcgagggaggagcaggtcaagcgctcgccgcacatccgcgacgccgtctggggctctctccggagcaccgactcgtacaccgtgctccactactactccaacatatgccggctgctgcggttcgacgacggcagggagatgtacgccaagttcaagctccgtcctgctgatccagacgtccccgaggactccggcaaggtggtgccgaggggcatcctgccgccggagaccggcgcgatccccagggacgaggatgacacgcgccctctgctcttcctcgccgacgacttccgccggagagtgggctcaccggacggggtgcgctacgtgttccagctgcagctcagggaggtgcccaccgacgccgccgcccgcgacgtcgccctcgactgcacccggccgtgggacgaggccgagttcccttacatcgacgtcggcgaggtcagcatcggccgcaacctcccgacggaggagacggagaagctcgagttcaacccgttcctccggtgccccgaggtggacgtcatcccggcgacgtcgtgcgcgcagagcgcgtccatcgaccacggccgctcgctggtctacgagatatgccagcgcctgcggaacggcgagccgctgcccgcgtcgtggcgagctttcctggagcagtcggacaccaagatcgacctgtcaggatgcccggtcgctgccgccgcgacacccacaaggtctaacgccggcgacgccacgaaggtgacgctggccaggacgtggtaccaggcgctctgggccacgctgtgccagccgctgctgcaaacgctggtgccgtactctgtcctgggcctcgtcatcttcctcccgctcaggggcctcctcgccgtcgccgcggccacccgcttcccgctctactggctcctcccggcgttctgggccgcgtcgggcgtcgccgccatggccacgtgcgcggcggcgaagtgggcgctggtcggcagcagggtcgacggcgacacggtgcacatctggtcgccggccgtgttcctggacacggtctggcaggccgtgcgcgccgccacggcggagtacttcgccgagctgacgcccgggtcggcgccgttcgcggcgtggatgcgggtgatgggcgcctccgtgtcccccggcgacggcgtgtacgtggactccatgggcgcgctgctgaacccggagatggtgcgcctggagcgcggcgccgccgtcgggcgggacgcgctgctgttcggccacgtgtacgagggcgaggccgggaaggtgaagttcggcgccgtgtccgtcggggaggacgggttcgtcggcagccgcgccgtggccatgcccagcgtcacggtggacgacggcggctgcctcgccgcgctcgggctggccatgaagggggagacggtgaagcacagcatgtaa</cdnaseq>|<br />
AA = <aaseq>MEERRATSPSRHSFCRDALGRRGGESGGGLVYIYRSPLRSPLQR HGLNREWRLERWRWRWRWEAATWRQRAAAHARIGEMDPKRSIDDQFSKLHPSLPVDTR IGIVGAGPSGLSAAYALAKLGYRNVTLFEKCHTVSGMCESIDIEEELGSDFEEMDTHK LSLIDSQTGNIRDLEVAEDYVSMVSLTLKLQDEANKSGRAGLHALSGLASDPTHEFLK QNGINSMPKSVAYGYTASGYGFVQDMPYAFIQEFTRTSMAGKIRRFKHGYMSMWERLS KSLPFEVFCDTQVLNVKRNSCGANVTIKNNNGEKQVLEFDKIILSGAVAFKNSKTYRS SSLTDGESEVVELNNLERELFSKVQTIDYYTTVVKINGFEHIPKGFYYFGEFMEDPTT IGHPVAMQRFFADTNIFLFWSYGNSADIKGSFVAKCVTDVVSSMGGNVQKVILQRRFK YFPHVSSKDMKDGFYERLESQLQGFQNTYYVGGLLAFELTERNASYSIMAVCKHFAID GEGELTPYVKRLFPLSHNRNPSPPRDLGELEGVEFPDLPSLDGYLQYWGTHKVTEKNV IYTWINEEGKLMNRRTYQELHGNASYIAQKLLTSTKPVIKPGDRVLLIHLPGLEFIDA FFGCIRAGVIPVPVLPPDPMQSGGQALLKVENISKMCNAVAILSTSSYHAAVRAGYIK NIVTLAKRVQKCSAQWPDIPWIHTDSWIKNYRRSSDSFNSDTVLFTKPQPSDLCFLQF TSGSTGDAKGVMITHEGLIHNVKTMKKRYRSTSKTVLVSWLPQYHDMGLIGGLFTALV SGGTSVLFSPMIFIRNPLLWLQTINDYHGTHSAGPNFAFELVIRRLEAEKNKVYDLSS MVFLMIAAEPVRQKTVRRFIELTQPFGLSEGVLAPGYGLAENCVYVTCAFGECKPVFI DWQGRVCCGYVEQDDTDTLIRIVDPDSLTEHQEDGVEGEIWISSPSSGVGYWGNSEMS QRTFFNQLKNHPNKKFTRTGDLGRTIDGNLFITGRIKDLIIVAGRNIYSADVEKTVES SSEVLRPGCCAVVGIPEEVLAQKGISIPDSSDQVGLVVIAEVREGKAVSKEVVNNIKA RVVEEHGVAVASVKLIKPRTICKTTSGKIRRFECMRQFVDNTLSLAKGNHVSKKKGLF RSLTTGTGMESKRSLLRQTVDLTISHWPKSQVKNSNEITEFLTQIVSEHTGISKDKIS LTDSLPSYGFDSIAVVRAAQKLSDFLGVPVGAIDIFTASCISELASFLENLVHKSQTQ LAPWPKSKVKNSKVIIEFLTKIVSDQTGIPKDKISPMDSLPSYGFDSIAVVQAAQKLS DFLGVPVGAIDIFTAGCISELATFLENLAHKSQSQLAPGASCYIEDETQVDPMDAISP EFSVLGTGILQLLALTYVCFVLLLPAYLASSTYMSIFSTVSLVRSPLLSYLSSLVMAP IVWIFYISLTSLSLSILGKSFLQPNYVLIPDVSIWSVDFVKWWALNKAQALAAKMLAV HLKGTIFLNYWFKMQGARIGSSVVIDTVDITDPSLLTVADGAVLAEGALVQGHEVCNE VLSFRPIWIGCEASIGPYAVLQKGTVVEDGAVVPPLQKTGAGKSTRRTSRTSVSIKKE AAKANMILEHLVSIYAVGILGALSGAIVYTLYTHLSGKAASPLHFSFACIAGAFHWLP AAITAYAVIVQETPTSALSFALFTAFADLSYGVILSILTSITSRALAAKPGTKQNGIA SLIHRRITISAHVRFAKMLSGTEAFCVYLRLLGAKIGRHCSIRAINPVANPELISVGD GVHLGDFCNIVPGFYSKGGFTSAEIKVQENTVVGSGSLLLPGCVLQENVILGALSVAP ENAVLRRGGVYVGSQSPAMVKNTLLDEDERIEEMDQAYKKIVGNLAANLAITTMNVKS RYFHRIGVSGRGVLRMYEEIPSFPRHKIFASGKSFPVIVRHSNSLSADDDARLDARGA AVRILSDNDGEAPLLDLTLKSGKAFYARTIADFATWLVCGLPAREEQVKRSPHIRDAV WGSLRSTDSYTVLHYYSNICRLLRFDDGREMYAKFKLRPADPDVPEDSGKVVPRGILP PETGAIPRDEDDTRPLLFLADDFRRRVGSPDGVRYVFQLQLREVPTDAAARDVALDCT RPWDEAEFPYIDVGEVSIGRNLPTEETEKLEFNPFLRCPEVDVIPATSCAQSASIDHG RSLVYEICQRLRNGEPLPASWRAFLEQSDTKIDLSGCPVAAAATPTRSNAGDATKVTL ARTWYQALWATLCQPLLQTLVPYSVLGLVIFLPLRGLLAVAAATRFPLYWLLPAFWAA SGVAAMATCAAAKWALVGSRVDGDTVHIWSPAVFLDTVWQAVRAATAEYFAELTPGSA PFAAWMRVMGASVSPGDGVYVDSMGALLNPEMVRLERGAAVGRDALLFGHVYEGEAGK VKFGAVSVGEDGFVGSRAVAMPSVTVDDGGCLAALGLAMKGETVKHSM</aaseq>|<br />
DNA = <dnaseqindica>7294..9882#3309..6578#3033..3211#2593..2952#2066..2507#1820..1953#1470..1673#1..235#atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggaggtatggattccccttccccactcgctgcgtgatgaaccgatgaaccgtagtacttgatgatatgcctgatttgcttcggcattgtgccgcttctctgttagctgggagtttgtggcgtgtcgtttaactgaagtagagatgtgatgatgatctgaggtgagcgccattttttttttttggctaactctgtttgttctattcagtgaatatgagctctcttttttgtcccctttttatcattctgcttctatgggtcgcggagcgttcttggttggtgcagtgcaactgcaatgtcagtatgcatgccatagtaatgtgatggtgggatgtaatgctcctctgcttgctcgagctctttcttggttgtctatctatcttggatgatttgatcaatgtactacttattatctgctgatagtacccggggtccatttgcgctagtattgaacttcttcttctcttataatttgctgaaatcgcgagattatcgcttaggtttatcaatcagaacattatcatgtttatgcttcggttcgttcggtactggcacatgtcaaataagcggaaattctgctactttttcggcttaccatgaatggcacccaacatagcgtggttgttttgtcatgttgaactagtacaaatttttgcaccttaatatttgctttccgtgcatatccaattaacctgcccatcaaacttcttttcagatagctattattggggaaaaatattttcccagcttcttttatagtgactagactgtttatacatgcctgcgtaattgcttccttttgatgagctagtacattatagaccttgattttgttttgatggtacgggttggttattggtgtccatataatgaaattgcaacttgcgaccactccatgatagttacctggtcattccttgttggttaataagctattatttcaaatatgaatgttcatcctacttgtcatattatagacatttgtcacaattcaagtgaggaagaaattctgtaattttctatgggctagttgctgcgttaactacaataatggggctgacatcctgaggttatagtgttcgattaccaagttctaatttattaagaggaatgcaagtcaacatgcatgcctagcccatttacttttaatgagtagtgtaacttctgttgcagaagtactaatgacagctttctatataaatttcacaggttgatttatcaaggacaacaattcgtcagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaaggtgaggaaactactcagcaaaattatacagtcatcattttgtgcacacttttagttatcagcaacattcgtatgtaggacgaatatatgatttgggaggacaagttatagcagcaaacagtgcccctgttataactcaccttgcagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggtgagttgttctagaataaaatttaaatttgaggtaactggataataagcatctaattatttataatattctctctatttcttgcatgcgtgttattcaataattcttacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactggtgagcatattttaaattcagtttggctggcattgaagctatagtatttcagttgccttattagtatgtatgatcttttgttcagatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaaggtatatatgttaataatatttctcctgttgcaaggtcttcttttttctcgtgataacattggtaatttcctgcattgcagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaaggtgagcaagaaataatggcgtaatatatctcaattattaattccaccttcatcaaatttcttagagaaattgtgcatatatttttccatacttgcagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttctggctcagaaaggcatctcaattccagattcttctgatcaggttggacttgttgtgattgcagaggttagagagggtaaggcagtctctaaagaggttgttaacaacatcaaagcacgtgtggtggaggagcatggtgtagctgttgcatctgtgaagcttatcaagcctagaaccatatgcaagacaacatctgggaagatacgaagatttgaatgcatgaggcagtttgttgacaatactcttagtttggctaagggcaaccatgtatctaagaaaaagggtctttttcggtctctgacaacaggaactggcatggagtcaaagaggtccctcttgaggcaaactgttgacctcaccattagccattggcccaaaagccaagttaaaaactccaatgaaataactgagtttctgacacaaatagtgtcagagcacactggcatttcaaaggataaaatttctctaactgacagtctaccttcgtatggttttgattctattgcagtggtacgagcagcccagaagctctcagattttcttggtgttcctgttggggccattgacatctttacagcaagctgcatttcggagcttgcaagcttcttggagaatttggtgcataagtcacaaacacagttggcaccttggcccaaaagcaaagtgaaaaactccaaggtgattatcgagtttctgacaaagattgtatcagatcaaacagggattccaaaggataaaatttctccgatggatagcctaccttcatatggttttgattcaattgcggtggtccaagcagcacagaaactctccgattttctcggtgtacctgttggagccattgacatatttacagctggttgcatttctgagctagctaccttcttggagaatttggcgcataagtcacagtcacagttggcacctggtgcatcttgctatattgaggatgagactcaagtggatcccatggatgccattagtccagaattcagtgttttgggtacgggcatcctgcaactgctggctcttacttatgtctgttttgtgctgctactcccagcatatcttgctagttcgacatacatgagcattttctctacagtcagtctggtcagatcaccattgctgagctatcttagttctttggttatggcacctattgtgtggatcttttatatctcgctcacttctttatcactgtctatcttggggaagtccttcctacagccaaattatgttctgatccctgatgtttcaatatggtcagttgactttgtgaaatggtgggcactaaacaaagcacaagcattagctgcaaagatgctagctgtgcatctaaagggcacaattttcttaaattattggttcaagatgcaaggagctcggattggttcatctgttgtcatcgacacagtagacataaccgatccatccctgttgacggttgcagatggtgcagttcttgcagaaggcgctcttgttcagggccatgaagtctgtaatgaagtgctgagttttaggcctatctggattggctgtgaggcttcgattggtccttatgcggtgcttcagaagggaactgttgtggaagatggagctgttgttccaccattgcagaagactggggctgggaaatcaacacgtcggactagcagaacttctgtaagcataaaggtaactaaagtatgtagacttcataatctacttcggtgtaataaaactatgatttgttatggtatacaacgatagtttgttccttcctgaccagaaacgtatagttgattatttctagacattgaatatagtaagctacagacttataattacaccataggccatttgaatttccttgtggtctttaacatggatgaagctgccaccaaagaacttccctacctttttacatttagtgaaaatattttttgagattcaggaagggacatatattttatcagggaaccataagcaaatcatttatgtacaagtggtcaagcagaaaaatcatataaattaaacaagatgaagggtttgtttggattgaagtcaatcttttggcaacttcaatagtattgtatagatatttggtccgataccaaatttttcccacactagtgcaaattctactcttaacaaatttggtagtgccgaaatttaccaatgttttggcacttccagtattttggtaagacaaaaaaatggcttcaaaccaaactagcccaaactcttttctgcatggtgcatagtgtctgcgtgtgtgcattttcattcgacagtggggtatatatctaactgccttcaaagttctcgattgcttctgcagaacatgtttgttcatggccatatttatataatgttattcgaatacaaaccgagtattttttctttgcagaaagaagcagccaaagcaaacatgatacttgaacacctcgttagcatctacgcagttggcatcctgggtgctctgtccggtgcaatcgtctacacactatacactcacttgtccggcaaagcggcttcgcctctgcatttctcctttgcatgcattgctggtgcgttccactggttaccagctgccatcactgcctatgctgttattgtccaggagacacccacaagtgcactgtcatttgctctcttcactgccttcgccgacctgagctatggtgtcatcctcagtattctcaccagtataaccagcagagcccttgctgcaaagcctggaaccaagcagaatggcattgcgagtttgatccaccggcgcataaccatctctgctcatgtcaggtttgccaagatgctatctggaacagaagccttctgcgtgtacctgaggcttctcggagcaaagattggcaggcattgctctatccgagccatcaatccggtggcgaatcctgagctgatcagcgtcggtgatggcgtccatctaggtgacttctgcaatattgttccagggttctactccaaggggggattcaccagtgcagagattaaggtccaggagaacaccgtggttggcagtggcagcttgctcctccctggctgtgtcctccaggagaatgtcatcctcggcgcgctctccgtcgcgccggagaacgctgttctccggcgtggcggcgtctacgtcgggtcacagtctccggccatggtgaagaacaccttgcttgacgaggatgaacggatcgaagagatggatcaggcatacaagaagattgttggcaacctcgcggcgaacttggccatcaccaccatgaacgtcaagtcgaggtacttccaccgcatcggcgtgagcggccgcggcgtcctgaggatgtacgaggagataccgtcgtttccccggcacaagatcttcgcctccggcaagtccttcccggtgatcgtccgccacagcaacagcctgagcgccgacgacgacgcgaggctcgacgcccgcggcgccgcggtgcggatcctctcggacaacgacggcgaggcgcctctcctcgacctcacactcaagagcggcaaggcgttctacgcgcgcaccatcgccgacttcgccacctggctggtctgcggcctgccggcgagggaggagcaggtcaagcgctcgccgcacatccgcgacgccgtctggggctctctccggagcaccgactcgtacaccgtgctccactactactccaacatatgccggctgctgcggttcgacgacggcagggagatgtacgccaagttcaagctccgtcctgctgatccagacgtccccgaggactccggcaaggtggtgccgaggggcatcctgccgccggagaccggcgcgatccccagggacgaggatgacacgcgccctctgctcttcctcgccgacgacttccgccggagagtgggctcaccggacggggtgcgctacgtgttccagctgcagctcagggaggtgcccaccgacgccgccgcccgcgacgtcgccctcgactgcacccggccgtgggacgaggccgagttcccttacatcgacgtcggcgaggtcagcatcggccgcaacctcccgacggaggagacggagaagctcgagttcaacccgttcctccggtgccccgaggtggacgtcatcccggcgacgtcgtgcgcgcagagcgcgtccatcgaccacggccgctcgctggtctacgagatatgccagcgcctgcggaacggcgagccgctgcccgcgtcgtggcgagctttcctggagcagtcggacaccaagatcgacctgtcaggatgcccggtcgctgccgccgcgacacccacaaggtctaacgccggcgacgccacgaaggtgacgctggccaggacgtggtaccaggcgctctgggccacgctgtgccagccgctgctgcaaacgctggtgccgtactctgtcctgggcctcgtcatcttcctcccgctcaggggcctcctcgccgtcgccgcggccacccgcttcccgctctactggctcctcccggcgttctgggccgcgtcgggcgtcgccgccatggccacgtgcgcggcggcgaagtgggcgctggtcggcagcagggtcgacggcgacacggtgcacatctggtcgccggccgtgttcctggacacggtctggcaggccgtgcgcgccgccacggcggagtacttcgccgagctgacgcccgggtcggcgccgttcgcggcgtggatgcgggtgatgggcgcctccgtgtcccccggcgacggcgtgtacgtggactccatgggcgcgctgctgaacccggagatggtgcgcctggagcgcggcgccgccgtcgggcgggacgcgctgctgttcggccacgtgtacgagggcgaggccgggaaggtgaagttcggcgccgtgtccgtcggggaggacgggttcgtcggcagccgcgccgtggccatgcccagcgtcacggtggacgacggcggctgcctcgccgcgctcgggctggccatgaagggggagacggtgaagcacagcatgtaa</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001187049.1 RefSeq:Os04g0473900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 4]]<br />
[[Category:Chromosome 4]]<br />
<br />
==References==<br />
<references><br />
<ref name="ref1">Xiaoyi Zhu, Lizhong Xiong.（2013）Putative megaenzyme DWA1 plays essential roles in drought resistance by regulating stress-induced wax<br />
deposition in rice.PANS 110(44): 17790-17795.</ref><br />
<ref name="ref2">Marahiel MA, Stachelhaus T, Mootz HD.(1997) Modular peptide synthetases involved in nonribosomal peptide synthesis.Chem Rev 97(7):2651–2674.</ref></div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0649700&diff=169621Os02g06497002014-05-19T03:02:06Z<p>Daweih: </p>
<hr />
<div>This gene encodes ATP-dependent zinc metalloprotease FTSH 7, chloroplastic, homologue of AtFtsH7. It is a predicted gene base on homology comparison.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Please input function information here.<br />
<br />
===Expression===<br />
Please input expression information here.<br />
<br />
===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Please input related labs here.<br />
<br />
==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os02g0649700|<br />
Description = Peptidase M41, FtsH extracellular domain containing protein|<br />
Version = NM_001054119.1 GI:115447608 GeneID:4330159|<br />
Length = 6155 bp|<br />
Definition = Oryza sativa Japonica Group Os02g0649700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br />
AP = Chromosome 2:27043137..27049291|<br />
CDS = 27043333..27044217,27045333..27045398,27045492..27045626,27045737..27045802,27045884..27046069<br>,27046145..27046231,27046353..27046466,27046542..27046666,27046743..27046851<br>,27047666..27047791,27047879..27048199,27048514..27048637,27048773..27048897<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008395:27043137..27049291<br />
source=RiceChromosome02<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008395:27043137..27049291<br />
source=RiceChromosome02<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggcctccgcctccgccgccgccgaaaccctcgcggcggcgtcgctccccgtcgcgtcgccttcgcggtcgctgctgcggccgctccccaggagagcgtccgcgggaggcggctgctccgcgtccgtacgaatctccgccgtgccgccgcgggggctcgggttcgcggtcgtccagcgccgcgtcctccgccgcccgccggcggcgcgcgccaacgtcgagcgcgaaggggacggggccgaagcgtccgggccaggagaggcgtcgtcctcctcctctggagatggggaccgcgacggggcggcggcggcggcggaggcaggaggtgatggcgccagcacgagcaccacgagcgcggccgcgacgcctccgcagccgccctcatcgaagaggggggagaacaagtggcggcggaagctgattaagggcggaggcgttgggcggtggctttgggagcccatagttcaggggagggaaatggggttcctcctgctccagcttgggttcgccatattcgcgctccgaatgctgcggcccgagatcgcgctgcctggctcagagccccggccacagacaacgtacgtcagcgtcccatacagcgacttcctggcgagcatagacaagaaccaggtgaagaaggtcgaggtggacggggtgcacatcatgttcaggctccgtcctgaggttgaggcccgtgcaatggagcagccacaggtgcagcgtggaacagattctgttgctgataatgctggagttccaaggcgaattgtcttcacaacaacacgtccagtggacattaagacaccatatgaaaaaatggtggagaatagtgttgagtttgggtcgccggacaaaaggtctggtggcttgctcaattcggctttggtggctcttatttatgttgtattaattgctgtagttctgcagcgattgccaataagcttttctcagcattcagcaggccagctgaggaatcggaagaattcaaactctggtggggccaaagtttcagaaagtaccgatatagttacatttgctgatgtggctggagtagatgaggccaaagaagagctggaagagattgtggagttcctaaggaacccagaaagatatattcgtcttggtgcgcgacctccacgaggtgtcttgttggtgggccttccaggaactggaaagacgcttcttgcaaaagctgtcgcaggggaagctgaagtcccattcataagttgctctgcaagtgaatttgtggagttgtatgtaggcatgggagcagctcgagtgcgtgacctatttgctagagctaaaaaggaatctccttcgattatttttattgatgagattgatgctgtggcaaaaagccgtgatggccgttatcgtatcgtcagtaatgatgagcgagagcagacactaaatcaattactcacggaaatggatggatttgacaccaactcagctgtcattgtacttggagcaacaaaccgagcagatgttttagatcctgccctccgacgccctgggagatttgaccgtgtagttatggttgaagctcccgacagatttggaagagaatctattctgaaagttcatgtgagcagaaaggagcttccgcttggtaaagatgtagatcttagtgacattgctgcaatgacaactggttttactggggcagaccttgcaaatttagtaaatgaagctgctttgttggctgggagatcaaataaagaaatagtggaaaaaattgacttcatctgtgcagtggagcgctctattgctggcattgagaaaaagcatgcaaagctgaagggtaatgaaaaggctgttgttgcacggcatgaagttggtcatgccgttgtgggaactgctgtagcaaacctattgccagggcagccacgtgtggagaaattgagtatattgccaaggtcaggtggtgcattgggctttacatacacccctcccacaacagaagatagatatttgctctttgttgatgaactgcgtggacgtctagtaacacttttgggtggaagggcagcagaggaagtagtactgtcagggcgtgtttctactggagcacttgatgacataaggcgtgccactgacatggcatacaaggctgtagcagagtatggtcttaatcagcgcataggtccgatatcggtggcaacattgtcaaatggtgggctagatgaatccggaggctctccatggggaagggatcagggccatctagttgaccttgtccagcgggaagtaaaagcccttcttcaatcagcccttgatgtcgctctttcagttgtccgtgctaatcctacagttttggaaggccttggagcatatttagaagagaatgaaaaggttgagggcgaggagcttcaagagtggctcaagtcggttgttgcaccaaaagaattgacctcatttataagaggaaagcaagagcaggttcttcagctggaagcaggctcctag</cdnaseq>|<br />
AA = <aaseq>MASASAAAETLAAASLPVASPSRSLLRPLPRRASAGGGCSASVR ISAVPPRGLGFAVVQRRVLRRPPAARANVEREGDGAEASGPGEASSSSSGDGDRDGAA AAAEAGGDGASTSTTSAAATPPQPPSSKRGENKWRRKLIKGGGVGRWLWEPIVQGREM GFLLLQLGFAIFALRMLRPEIALPGSEPRPQTTYVSVPYSDFLASIDKNQVKKVEVDG VHIMFRLRPEVEARAMEQPQVQRGTDSVADNAGVPRRIVFTTTRPVDIKTPYEKMVEN SVEFGSPDKRSGGLLNSALVALIYVVLIAVVLQRLPISFSQHSAGQLRNRKNSNSGGA KVSESTDIVTFADVAGVDEAKEELEEIVEFLRNPERYIRLGARPPRGVLLVGLPGTGK TLLAKAVAGEAEVPFISCSASEFVELYVGMGAARVRDLFARAKKESPSIIFIDEIDAV AKSRDGRYRIVSNDEREQTLNQLLTEMDGFDTNSAVIVLGATNRADVLDPALRRPGRF DRVVMVEAPDRFGRESILKVHVSRKELPLGKDVDLSDIAAMTTGFTGADLANLVNEAA LLAGRSNKEIVEKIDFICAVERSIAGIEKKHAKLKGNEKAVVARHEVGHAVVGTAVAN LLPGQPRVEKLSILPRSGGALGFTYTPPTTEDRYLLFVDELRGRLVTLLGGRAAEEVV LSGRVSTGALDDIRRATDMAYKAVAEYGLNQRIGPISVATLSNGGLDESGGSPWGRDQ GHLVDLVQREVKALLQSALDVALSVVRANPTVLEGLGAYLEENEKVEGEELQEWLKSV VAPKELTSFIRGKQEQVLQLEAGS</aaseq>|<br />
DNA = <dnaseqindica>197..1081#2197..2262#2356..2490#2601..2666#2748..2933#3009..3095#3217..3330#3406..3530#3607..3715#4530..4655#4743..5063#5378..5501#5637..5761#gccggcctcgcctcggcctctgccttcctcatctctccccctctctctctctctctctctccctcctcctcctcaccccgacccctccccgccgattcctccctcctccacgcgcgcgcggcgcggcgcagcgagcgctctccgattcttccggctccgagggattagggttccgggggggcacgcgtccgctgacatggcctccgcctccgccgccgccgaaaccctcgcggcggcgtcgctccccgtcgcgtcgccttcgcggtcgctgctgcggccgctccccaggagagcgtccgcgggaggcggctgctccgcgtccgtacgaatctccgccgtgccgccgcgggggctcgggttcgcggtcgtccagcgccgcgtcctccgccgcccgccggcggcgcgcgccaacgtcgagcgcgaaggggacggggccgaagcgtccgggccaggagaggcgtcgtcctcctcctctggagatggggaccgcgacggggcggcggcggcggcggaggcaggaggtgatggcgccagcacgagcaccacgagcgcggccgcgacgcctccgcagccgccctcatcgaagaggggggagaacaagtggcggcggaagctgattaagggcggaggcgttgggcggtggctttgggagcccatagttcaggggagggaaatggggttcctcctgctccagcttgggttcgccatattcgcgctccgaatgctgcggcccgagatcgcgctgcctggctcagagccccggccacagacaacgtacgtcagcgtcccatacagcgacttcctggcgagcatagacaagaaccaggtgaagaaggtcgaggtggacggggtgcacatcatgttcaggctccgtcctgaggttgaggcccgtgcaatggagcagccacaggtgcagcgtggaacagattctgttgctgataatgctggagttccaaggcgaattgtcttcacaacaacacgtccagtggacattaagacaccatatgaaaaaatggtggagaatagtgttgagtttgggtcgccggacaaaaggtctggtggcttgctcaattcggctttggtaaggcactacagctgaaaaataaatttgttatacgtgtggtatttttaggctggatatcttagtatagggaaagcaataatcacatcagaacatagatagggaagaatgcagtatagcatgtagaatgatggttattgtaggactggtgtggtgttgagttttgaaatggagagatcaattagttgcagtgacttatgcaaactagttattttgtggcttatagcactatatacagcatacttaccactggccagccatgaaggagaagaaaatgctgtgtgtttaactgtttactattagcatgaaggctcgagatatttgcaactgcgtgagagcatataattcatgttagtttcaacttatggaaaatcagaagcatcattgctcaatttgctaacagaagactcagaaatcacatattccatggtgtttggtgatagttgaaatgatgttttatagacatggtagttcagagttaaaagtttactatatttgtgccctggtcacagataagccgattattacaagaacataaattatagtcagttgcatatcacaaaaaatcctcgcatcaatatgccaaataaatcttcggatatgacctgaaataatggaattatctgaggattcatgtgggtatttctacatgcagttgtaatattacgcagtagattgagtttctaacctgacttcaaatgcaaattgaaagcacaacttttaacattaagactttaaaatcactagtacttttcttcgttattatcatattcccctcgtcccttcatccttgtgaactcttcattcatcaccactgttgtataccttgaatgagaaaacaggatggaatgatttatggattggttcacacgaaaaactgaacttccagttgaatgttggaacttacagatcaatgaattaatggggttttgttaaaacttagtatggcaaaatggaatcctaagtagaaaataggttacagagcataccatttatctgagacagcactgttttgctgtttcattttgttcctaacttcttctttattagaaatatacagctctctctcctagctgcgtttttttgtcctaaccatgcccttattttttttgcaggtggctcttatttatgttgtattaattgctgtagttctgcagcgattgccaataagcttttctcaggtactgatgaatgcacttcattacttcaatcaactaatgaacatactcggttatatctttaatagatataatatagtttcttgtgtatatcagcattcagcaggccagctgaggaatcggaagaattcaaactctggtggggccaaagtttcagaaagtaccgatatagttacatttgctgatgtggctggagtagatgaggccaaagaagagctggaagagattgtggtatgtgttttatgctcaatgttactggtatatgcagttatccactgatattatgccttatctgttgactcgaggggaataagctagttatatcttaatcttatgttcaggagttcctaaggaacccagaaagatatattcgtcttggtgcgcgacctccacgaggtgtcttgttggtcagttctgcagtcatttaagtaatgatgaatgttgctggactagagtttttgtactatgatgtaattcattatttacaggtgggccttccaggaactggaaagacgcttcttgcaaaagctgtcgcaggggaagctgaagtcccattcataagttgctctgcaagtgaatttgtggagttgtatgtaggcatgggagcagctcgagtgcgtgacctatttgctagagctaaaaaggaatctccttcgattatttttattgatgaggtaacctactgtttgaggacaaaaatctagttttatgtgcttaaatgtgtgataactaattgcatttctttgtagattgatgctgtggcaaaaagccgtgatggccgttatcgtatcgtcagtaatgatgagcgagagcagacactaaatcaattactcacggtattgatagtaaagagtcctttaatcttgtgattctgactatattttactcacatacacacatctttagttggggtccaattgaattatcttcgtgttaattttctctgaatctacctaggaaatggatggatttgacaccaactcagctgtcattgtacttggagcaacaaaccgagcagatgttttagatcctgccctccgacgccctgggagatttgaccgtgtagttatggtaaattgatcttctcaataaaaaaaagtgatactggatctctgttgtctgaggtcatagatatttatgtgataggttgaagctcccgacagatttggaagagaatctattctgaaagttcatgtgagcagaaaggagcttccgcttggtaaagatgtagatcttagtgacattgctgcaatgacaactggttttactgggtgagaattcttctgctcctttttaataagcatgcagcattttcagttgatttgacattctttttacactatacagggcagaccttgcaaatttagtaaatgaagctgctttgttggctgggagatcaaataaagaaatagtggaaaaaattgacttcatctgtgcagtggagcgctctattgctgtatgttcctttatctcaaattcgtttgctattattgtttgctaaggtcattttatacaaggaaatttcgtctgcttggtttcttatttctatcctagttttcttaaccgatttacaagaatatgtggaatggcaacttatacaagacaaatatctagatatccattttgaaactattcttgacttgtctctcccaattctgcttttgtagtataggtaataaagctgccagtgcattatgtccactaaatgcgttcggtttcttgcaatgaatttatgtctatttccacccttgctggtagtcaaattttttaaagtttttgaaataccgttcaacttgtgagttttttgatagattttggggccgtttggtagggctccaactcctaaatttaattccaagagttgggtctgaagtggatttgtggagctgcctaaacccagcttcacctctctagttcattttgtgagagcactccagccagctccactccattcattggtggagctgaaactgtttggttgggctccagctctaggagcggtggagtttgagctagagatatgccaaacaggccctttatggattttctttatttgataatggggacgccataaggtcatatatgatgttactgtcctcattcttttcctacatgctgtccattgattcagcactgaaggaatggcccttttgttgatccttgccctagttcaatattgttatggaagatgtatctgttccagctgttagagtagacattgttttacttccacattgtttcctggaaactaactggaattattttcttagggcattgagaaaaagcatgcaaagctgaagggtaatgaaaaggctgttgttgcacggcatgaagttggtcatgccgttgtgggaactgctgtagcaaacctattgccagggcagccacgtgtggaggtttgaacgcaagcaactatcgatagaacagtttaagaaaatgttcgtcttgggcttacattttagtgttgttcttttttccaccagaaattgagtatattgccaaggtcaggtggtgcattgggctttacatacacccctcccacaacagaagatagatatttgctctttgttgatgaactgcgtggacgtctagtaacacttttgggtggaagggcagcagaggaagtagtactgtcagggcgtgtttctactggagcacttgatgacataaggcgtgccactgacatggcatacaaggctgtagcagagtatggtcttaatcagcgcataggtccgatatcggtggcaacattgtcaaatggtgggctagatgaatccggaggctctccatggggaagggatcaggtctgtctcaaaatgtccctactcctttacttgaattttttatttggaatttggacattttcgcgggtacttttttttttaactttttagaaagtcaagttcttttttagaaagattacccgtctttgtccttgcctacgaatcagtccacattttgaacaaattttacgaacagaagctctttttttcatgtttccgtattcctttattaaactatgaatttactcttttggaaaaaaaaaagtctctttgcctgacaaatgcgtaagctaccatctgcatgtattgtgctcacactgcaaaactaatgacagggccatctagttgaccttgtccagcgggaagtaaaagcccttcttcaatcagcccttgatgtcgctctttcagttgtccgtgctaatcctacagttttggaaggccttggagcatatttagaaggtaaaaaactagaccaaattatcaggttgcatatgttattgtggctctcctttctatgatgggttttgcttcattagattaaaaatgaaaactgtttaacctatgcataacggctttcttctctaccccattcagagaatgaaaaggttgagggcgaggagcttcaagagtggctcaagtcggttgttgcaccaaaagaattgacctcatttataagaggaaagcaagagcaggttcttcagctggaagcaggctcctagcactatacattggtctcgtagaatcaattaattaatttaacacaagtagctccaacagtttaaactgtacaagttctgaggtacgcaactcacgcccaagccaagacccttgtgatgctgtatatatctatgtgccgctccacgacccttgactacaggtctaccaggaatgattcatagcgagttgtaaaccccggagtattgtaagttccttcggtttcctcgtttgccgttggatgttgtttatagattaggcgggtggtacacatgtaaaagaatattcgtttttcttctgtgttctttctctttctgttcatcctgcggaaggaattttttgttacattgtcatcttggcaaatattaataggaataattcatatacgtcgttgatcct</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054119.1 RefSeq:Os02g0649700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 2]]<br />
[[Category:Chromosome 2]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0458400&diff=169614Os05g04584002014-05-19T02:56:36Z<p>Daweih: </p>
<hr />
<div>This gene encodes ATP-dependent zinc metalloprotease FTSH 8, mitochondrial, homologue of AtFtsH3/10. It is a predicted gene base on homology comparison.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Please input function information here.<br />
<br />
===Expression===<br />
Please input expression information here.<br />
<br />
===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
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==References==<br />
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<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os05g0458400|<br />
Description = Similar to AAA-metalloprotease FtsH|<br />
Version = NM_001062280.1 GI:115464290 GeneID:4339002|<br />
Length = 5798 bp|<br />
Definition = Oryza sativa Japonica Group Os05g0458400, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br />
AP = Chromosome 5:22564129..22569926|<br />
CDS = 22564340..22564406,22564498..22564728,22564835..22564909,22565567..22565700,22566145..22566630<br>,22566810..22567577,22568323..22568550,22569250..22569729|<br />
GCID = <gbrowseImage1><br />
name=NC_008398:22564129..22569926<br />
source=RiceChromosome05<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008398:22564129..22569926<br />
source=RiceChromosome05<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgagcctcgcctcgctcgcccgcgcgctctcccgccgctccgcgccctcctcctcccgcgcgcgccagggtttctccctcggtgggctaggtgggaccaccaggtcgccgccgccgccgtcgtcgccgctcccatcgctccatggcggggagggcggtgggctgggattgggattcgtccgcgggtacctgacggcggcgctggggaggcccgccgcggtgaaggctgggacagactggaggtccatcctcgcgaacccgcagttccggaggctcttctctgacgggtccaagaagaattacgagaactactaccccaagggcaagaaggaggcgccgaagggggatgggagtaacaagtctgattcgaagcaagattcaagtacagacgatcaatggaatttccaggagactgcctcaaagcaattgcaaaattttctggcacctctactgtttcttggactgatgctctcgtccttgtcgtcgagctcatcagaccaaaaggagataagcttccaggaattcaagaataagctactagaacctggtttagttgaccgcattgttgtttcaaataaatcagtggcaaaggtctatgtcaggagttcaccacagtcgaacagccaaggtcagaacactgatgccatcattaccaccaatgatgttccgagcaagcatactcccagcagatacaagtattacttcaatattggaagtgttgattcctttgaagaaaagttagaggaagcccaggaagctttgggagtagatccacatgattttgttccagtaacttatgttgctgaagtgaattggttccaagaagtaatgagatttgccccaacagtctttcttgttgggctaatatatttgatgagcaaaagaatgcagagtggttttaatattggaggtggacctggcaagggtggccgtggtatttttaatattggaaaagctcaggtgacgaagatggacaaaaattccaaaaacaaggtgttttttaaggatgtagctggttgtgatgaagctaagcaagaaataatggagtttgtgcatttccttaagaatcccaagaagtatgaagagttaggagctaagataccaaaaggtgccttgcttgtgggtcctcctggaacaggcaagactctgcttgccaaagctacagcaggagagtctggtgtgcccttcctgtctatttctggatcagatttcatggaaatgtttgttggggttggaccatccagggtacggaacttattccaagaagctcggcaatgtgctcccagtattatatttattgatgagatcgatgcgattggtcgtgcaagaggccgtggagggttttctggttcaaatgatgagcgtgaaagtactttgaaccagctgcttgtagagatggatgggtttggtacaacttctggtgttgttgttcttgctggtacaaataggcctgatattttagataaagccttgctaaggcctggaagatttgatcgccaaataacaatagacaagccagatataaagggccgtgatcaaatattccgcatatatcttaaaaaacttaaacttgacaatgagccatcattttattcgcaaaggctagcggctttgacacctggatttgcaggagctgacattgccaatgtttgtaatgaagctgctttaattgctgcgagaagtgaggaaactcagattaccatgcaacattttgaatctgcaattgatagaattattggtggtttggagaagaaaaacaaggtcatcagcaaactggagcgccgcactgttgcttaccatgaatctggtcatgctgttgctggatggtttttagagcatgcagagcctcttctgaaagttacaattgttccccgtggaacagctgctttaggatttgcacagtatgttccaaatgaaaatcttttgatgacaaaagaacagctttttgatatgacatgcatgacattaggtggccgagctgcagaggaggttttgattggaaggatctccactggtgctcagaatgacctggagaaagttaccaaaatgacctatgcgcaggttgctgtatacggtttcagcgaaaaggttgggcttctgtccttccctcagagggacgatggctttgaaatgaccaaaccttacagcaaccaaacagcatccatcatcgacgatgaggtgagggaatgggttggcaaggcgtacaaaaaaacagtggaactgataactgagcacaaggagcaagtcgccaaaatcgctgaaatgctgcttgagaaggaggtcctccaccaggacgatttggttagggtattaggagaacggcctttcaaggcatccgagccaactaactacgacctcttcaagcaagggttccaggatgaagaagacagcaagaaccaagaggcggccaaaacccctcagcctgatgatgacggaacaccctcgcttggcgaggttgtacccacgtaa</cdnaseq>|<br />
AA = <aaseq>MSLASLARALSRRSAPSSSRARQGFSLGGLGGTTRSPPPPSSPL PSLHGGEGGGLGLGFVRGYLTAALGRPAAVKAGTDWRSILANPQFRRLFSDGSKKNYE NYYPKGKKEAPKGDGSNKSDSKQDSSTDDQWNFQETASKQLQNFLAPLLFLGLMLSSL SSSSSDQKEISFQEFKNKLLEPGLVDRIVVSNKSVAKVYVRSSPQSNSQGQNTDAIIT TNDVPSKHTPSRYKYYFNIGSVDSFEEKLEEAQEALGVDPHDFVPVTYVAEVNWFQEV MRFAPTVFLVGLIYLMSKRMQSGFNIGGGPGKGGRGIFNIGKAQVTKMDKNSKNKVFF KDVAGCDEAKQEIMEFVHFLKNPKKYEELGAKIPKGALLVGPPGTGKTLLAKATAGES GVPFLSISGSDFMEMFVGVGPSRVRNLFQEARQCAPSIIFIDEIDAIGRARGRGGFSG SNDERESTLNQLLVEMDGFGTTSGVVVLAGTNRPDILDKALLRPGRFDRQITIDKPDI KGRDQIFRIYLKKLKLDNEPSFYSQRLAALTPGFAGADIANVCNEAALIAARSEETQI TMQHFESAIDRIIGGLEKKNKVISKLERRTVAYHESGHAVAGWFLEHAEPLLKVTIVP RGTAALGFAQYVPNENLLMTKEQLFDMTCMTLGGRAAEEVLIGRISTGAQNDLEKVTK MTYAQVAVYGFSEKVGLLSFPQRDDGFEMTKPYSNQTASIIDDEVREWVGKAYKKTVE LITEHKEQVAKIAEMLLEKEVLHQDDLVRVLGERPFKASEPTNYDLFKQGFQDEEDSK NQEAAKTPQPDDDGTPSLGEVVPT</aaseq>|<br />
DNA = <dnaseqindica>212..278#370..600#707..781#1439..1572#2017..2502#2682..3449#4195..4422#5122..5601#ggtaaaacgccttctccccctcctcttccgccgccgcggcgatccctctttcacctccacctccacctgctcaggacgcggccacgcagtagtcgcggagaaaagaaaccgaaaagaaaaaaaaaaatctagctacgcatctcggcccggcccccccgccgccgcgcgctcggatcccccgacggctcgccgccacggcgccgccgccgccatgagcctcgcctcgctcgcccgcgcgctctcccgccgctccgcgccctcctcctcccgcgcgcgccaggcaagtctccgccctctccctcttatcttccctgaatcttccgccaccgcgtctgggtggggggctgattgtgtggatggcttttttttagggtttctccctcggtgggctaggtgggaccaccaggtcgccgccgccgccgtcgtcgccgctcccatcgctccatggcggggagggcggtgggctgggattgggattcgtccgcgggtacctgacggcggcgctggggaggcccgccgcggtgaaggctgggacagactggaggtccatcctcgcgaacccgcagttccggaggctcttctctgacgggtccaagaagagtgagtcccatccgcccttttttgatcggttcggatggatggctttaggtgctaaaagtttgtggttgtgtttttgtgtgtgtgtgtgtatttttatgatttttagattacgagaactactaccccaagggcaagaaggaggcgccgaagggggatgggagtaacaagtctgattcgaagcgtaagttgccgtcttttttttctcggttgcatcatttgtttgtgtggagaatggtgatcatgttgtgaaattaatagaatgcactgcacagcttttaatacaatagcatgtaactggtgtactgattgtcattgaaccaacgaggtgaataatcaggacatgttatttttcttgcgctgcaagtgcttggtcgactgatgccaaatggaaaaatagcttgtgtagtgcaaccaatgatggcttgtgagcctatggtgtacaaccacttacttgtcagtgttcttaaaaaacgaatgcaaactagatccttgttcagttttaaaaaaatgcagggatacactgtaatttagtataaaaactcagaacctcagtggttatctaacttaagaggctgaaccgctgaaccaaataaacacagtagcacagaaagttccagattttggtctttgctagatcgttagttctctggaaattaaaacgtgccgctgacctagcactttcatcttaataatttctttgggagattagtacgggttttgatggagattggagcctagattttagtggctggaatgccaagagtccttttgagggcaacaaaataataaactcttagtgcatcattgcgtgtgggctaatgtctggtttgatatgcagaagattcaagtacagacgatcaatggaatttccaggagactgcctcaaagcaattgcaaaattttctggcacctctactgtttcttggactgatgctctcgtccttgtcgtcgagctcatcagaccaaaaggaggtaatttttcactcccttctgagttctgacagattatattcacttgttctgctatgcacatgaatctcttcctatatactagaaaaaagtggatggaagaaaactatgttgcgattataaggactataaaagttgtacttaccccaatcaaattactgcaatcagctattgagcattctgtaataaatagttcaatacaagcagtactagatccagatggcagcaatacattcttctcaccgtgaagttttatcttaaactgacaaatggtagagaaaatacctgccgctcctctttagatggtttcaccttatgcctgcaccagtcaacatatagcagacaactgattttgtggttgcatatttattctttttaaatctatggtgaacaattattctcgatcttaaatggaatctcaagatattttgcttcaaatttctgcagataagcttccaggaattcaagaataagctactagaacctggtttagttgaccgcattgttgtttcaaataaatcagtggcaaaggtctatgtcaggagttcaccacagtcgaacagccaaggtcagaacactgatgccatcattaccaccaatgatgttccgagcaagcatactcccagcagatacaagtattacttcaatattggaagtgttgattcctttgaagaaaagttagaggaagcccaggaagctttgggagtagatccacatgattttgttccagtaacttatgttgctgaagtgaattggttccaagaagtaatgagatttgccccaacagtctttcttgttgggctaatatatttgatgagcaaaagaatgcagagtggttttaatattggaggtggacctggcaagggtggccgtggtatttttaatattggaaaagctcaggtgacgaagatggacaaaaattccaaaaacaaggttagttcattctcttcttctgttctactttgaaacgactgtcaaatatgaaatatatacatgcacattgattctcaagctctgctagttccacctattcaataatgcagtagctactgcttaaacgttgttaagttacaataaaaactgtataatgacaagcttttctgttggtccaggtgttttttaaggatgtagctggttgtgatgaagctaagcaagaaataatggagtttgtgcatttccttaagaatcccaagaagtatgaagagttaggagctaagataccaaaaggtgccttgcttgtgggtcctcctggaacaggcaagactctgcttgccaaagctacagcaggagagtctggtgtgcccttcctgtctatttctggatcagatttcatggaaatgtttgttggggttggaccatccagggtacggaacttattccaagaagctcggcaatgtgctcccagtattatatttattgatgagatcgatgcgattggtcgtgcaagaggccgtggagggttttctggttcaaatgatgagcgtgaaagtactttgaaccagctgcttgtagagatggatgggtttggtacaacttctggtgttgttgttcttgctggtacaaataggcctgatattttagataaagccttgctaaggcctggaagatttgatcgccaaataacaatagacaagccagatataaagggccgtgatcaaatattccgcatatatcttaaaaaacttaaacttgacaatgagccatcattttattcgcaaaggctagcggctttgacacctggatttgcaggagctgacattgccaatgtttgtaatgaagctgctttaattgctgcgagaagtgaggaaactcagattaccatgcaacattttgaatctgcaattgatagaattattggtggtttggagaagaaaaacaaggtaatgtgaatatgtgattatgtgccagtttgaagataatctactccaaactgttaaagcaataaatgcaacctgcaaagctgattagtgcctgatttacacattactccagcattgtgtagttctgtatagtgctctcatgtgaaccggttatgctctattttgatacacattttttgctggtttaatgactatggaagttttgtttttttaaaaaaacaaaaacaaaagaacggtacataatgactagtgcattcattaagttcctgttctaggtggaccttaattaagttaagtgatttgtgaatgtggcatgatagttcgtgtagatcactagatggttgatcatgcatgaatgtttaggtgagagtgctgtaaacaaggtaaactatataaaataagtagtggatctacatgcaaatataaaaaactctaggtcttactcagaaatggatggtcacttaagtgtacgcaaaataatgatttctgaaatttgtggctttgcacacacaatatctgcagtatgtttcccttttggaatgcccaagccataactgttttttcacctttaacttgttccatagtaaatatataaaatatctactttcagtaagggagtaatgcacagatcaatgcttagtaacttagtaactttcttttatatgaaatgacttgttctcaacatgtgatagtcatgtttccccatttatttttcttacctcttagcctgttaatcaaattgcaggtcatcagcaaactggagcgccgcactgttgcttaccatgaatctggtcatgctgttgctggatggtttttagagcatgcagagcctcttctgaaagttacaattgttccccgtggaacagctgctttaggatttgcacagtatgttccaaatgaaaatcttttgatgacaaaagaacagctttttgatatgacatgcatgacattaggtggccgagctgcagaggaggtacacactaattcttttacagtcttatgactgatttatgttaatagctaccttttgtggatatatctttcaaattcctactgctgagtgctgacacaccatttcttttcagtcttcttatgacagcatgcgtgttatgtttttatgactagaacgaatacaaggtttttctgcatttgacagctgtaaaaaaattaaaaaaaaatggttaactaggttacagtttacacattcaattatgatgacttggtgaatacgagtaatattatcacaatttggtagtcaaactcttgttggtttggttacatcaagacttctaaccatttatgactctaaccagaaaggcaaattgatgtttgtgtgtcttcatattacactgttggttagcatgaattctttgatgtttaacatggtactttctttttttgcttggaccatatgcttatgtacggcgagactcgacatggattgccgtcatgccatttgattactttaacatcggtcctctctcattattttcagcttcttccatacattttacttattgtcatataacataatgaaataacaataagccaatccaagtgctgggttgtggtatttgttcattccatatttccatgattacgctgtcaaatcgttcacaatgttggcagtggaatgatacctaatgtagatgttttgattgccttgttacaggttttgattggaaggatctccactggtgctcagaatgacctggagaaagttaccaaaatgacctatgcgcaggttgctgtatacggtttcagcgaaaaggttgggcttctgtccttccctcagagggacgatggctttgaaatgaccaaaccttacagcaaccaaacagcatccatcatcgacgatgaggtgagggaatgggttggcaaggcgtacaaaaaaacagtggaactgataactgagcacaaggagcaagtcgccaaaatcgctgaaatgctgcttgagaaggaggtcctccaccaggacgatttggttagggtattaggagaacggcctttcaaggcatccgagccaactaactacgacctcttcaagcaagggttccaggatgaagaagacagcaagaaccaagaggcggccaaaacccctcagcctgatgatgacggaacaccctcgcttggcgaggttgtacccacgtaatcctgcagcaaatttacagctgtaaattgcactcctattcgatagattcttgtttgttgttgcagcataagaaaatttgtataaattaacgccctcgcctaatctctgatgtgggaacccagttggcttttcgccggccatagtgtttccctgttttaaatgaatacgaagagctagtcgaggatcatgatttgagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062280.1 RefSeq:Os05g0458400]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 5]]<br />
[[Category:Chromosome 5]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0229066&diff=169610Os06g02290662014-05-19T02:51:30Z<p>Daweih: </p>
<hr />
<div>This gene encodes ATP-dependent zinc metalloprotease FTSH 6 (OsFtsH6) in Oryza sativa, homologue of AtFtsH6. It is a predicted gene base on homology comparison.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Please input function information here.<br />
<br />
===Expression===<br />
Please input expression information here.<br />
<br />
===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Please input related labs here.<br />
<br />
==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0229066|<br />
Description = Twin-arginine translocation pathway signal domain containing protein|<br />
Version = NM_001187744.1 GI:297724618 GeneID:9268843|<br />
Length = 1461 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0229066, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:6701957..6703417|<br />
CDS = 6701957..6703417|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:6701957..6703417<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:6701957..6703417<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgtcgctgacgacgacgacgagccggctgccgatctgcagggcgcagggcggcggcgtcgccaaggagaagcggacgacgccgccacctgccaagatcacgccgccttcgtcgtcgtcgtcggaggccgccggactaagcaggaggaggctgctgcaatcggccggcctcgggctcggcctcggcctaacggcggcgaggccggcgcgagccgaggcgacggcgccggaggaggtgacgtcgaacaggatgtcctactcccggttcctggagtacctcgacgccggcgccgtcaagaaggtggacttcttcgagaacggcacggtggccgtcgccgaggtggacgacgcggcggcgctgtcgagggtgcaccgcgtcaaggtgcagctcccgggcctccccgccgagctcgtccggaagctccgggacaagggcgtcgacttcgcggcgcacccggtggagcccagcgccggggtgatgctgctcgacctcctcgtcaacttcggcttccctctcctcttcgtcgcctcgctcctgtggcggtcgccgaccatgaacaaccccggcggcgggcctagcctcccgttcgggctcggcaagtccaaggccaagttccagatggagcccaaaaccggcgtcacgttcgacgacgtcgccggcgtggacgaggccaagcaggacttccaggagatcgtccagttcttgaagttcccggagaagttcacggcggtcggtgcgaggacccccaagggcgtgctgctggtcggcccgccggggacggggaagacgctgctggccaaggcgatcgccggcgaggccggcgtgcccttcttctcgctgtccggctccgagttcatcgagatgttcgtcggcgtcggcgcgtcgcgggtgcgcgacctgttcgacagggccaaggcgagcgcgccgtgcctcgtcttcatcgacgagatcgacgccgtggggcggcagcgcggcgcggggatcggcggcgggaacgacgagagggagcagacgctgaaccagctgctcaccgagatggacgggttcggcggcggcgacggcggcgtcgtcgtgatcgccgccaccaaccggccggagatcctcgacgcggcgctgctccggccggggcgcttcgaccggcgggtgtccgtcggcctccccgacgtgcgcggacgggaggagatcctcctcgtgcacggcgccaacaagcggctcgaccccggcgtgtcgctcgccgtggtcgccatgcgcacgccggggttcagcggcgccgacctcgccaacctcatgaacgaggccgccatcctcgccggccgccgcggcaaggaccgcatcaccgtcagcgagatcgacgactccatcgaccgcatcgtcgccggcctcgagggcaccagcatgaccgacggcaagagcaagatgctcgtcgcctaccatgagatcggccacgccgtgtgcgcgtaa</cdnaseq>|<br />
AA = <aaseq>MSLTTTTSRLPICRAQGGGVAKEKRTTPPPAKITPPSSSSSEAA GLSRRRLLQSAGLGLGLGLTAARPARAEATAPEEVTSNRMSYSRFLEYLDAGAVKKVD FFENGTVAVAEVDDAAALSRVHRVKVQLPGLPAELVRKLRDKGVDFAAHPVEPSAGVM LLDLLVNFGFPLLFVASLLWRSPTMNNPGGGPSLPFGLGKSKAKFQMEPKTGVTFDDV AGVDEAKQDFQEIVQFLKFPEKFTAVGARTPKGVLLVGPPGTGKTLLAKAIAGEAGVP FFSLSGSEFIEMFVGVGASRVRDLFDRAKASAPCLVFIDEIDAVGRQRGAGIGGGNDE REQTLNQLLTEMDGFGGGDGGVVVIAATNRPEILDAALLRPGRFDRRVSVGLPDVRGR EEILLVHGANKRLDPGVSLAVVAMRTPGFSGADLANLMNEAAILAGRRGKDRITVSEI DDSIDRIVAGLEGTSMTDGKSKMLVAYHEIGHAVCA</aaseq>|<br />
DNA = <dnaseqindica>1..1461#atgtcgctgacgacgacgacgagccggctgccgatctgcagggcgcagggcggcggcgtcgccaaggagaagcggacgacgccgccacctgccaagatcacgccgccttcgtcgtcgtcgtcggaggccgccggactaagcaggaggaggctgctgcaatcggccggcctcgggctcggcctcggcctaacggcggcgaggccggcgcgagccgaggcgacggcgccggaggaggtgacgtcgaacaggatgtcctactcccggttcctggagtacctcgacgccggcgccgtcaagaaggtggacttcttcgagaacggcacggtggccgtcgccgaggtggacgacgcggcggcgctgtcgagggtgcaccgcgtcaaggtgcagctcccgggcctccccgccgagctcgtccggaagctccgggacaagggcgtcgacttcgcggcgcacccggtggagcccagcgccggggtgatgctgctcgacctcctcgtcaacttcggcttccctctcctcttcgtcgcctcgctcctgtggcggtcgccgaccatgaacaaccccggcggcgggcctagcctcccgttcgggctcggcaagtccaaggccaagttccagatggagcccaaaaccggcgtcacgttcgacgacgtcgccggcgtggacgaggccaagcaggacttccaggagatcgtccagttcttgaagttcccggagaagttcacggcggtcggtgcgaggacccccaagggcgtgctgctggtcggcccgccggggacggggaagacgctgctggccaaggcgatcgccggcgaggccggcgtgcccttcttctcgctgtccggctccgagttcatcgagatgttcgtcggcgtcggcgcgtcgcgggtgcgcgacctgttcgacagggccaaggcgagcgcgccgtgcctcgtcttcatcgacgagatcgacgccgtggggcggcagcgcggcgcggggatcggcggcgggaacgacgagagggagcagacgctgaaccagctgctcaccgagatggacgggttcggcggcggcgacggcggcgtcgtcgtgatcgccgccaccaaccggccggagatcctcgacgcggcgctgctccggccggggcgcttcgaccggcgggtgtccgtcggcctccccgacgtgcgcggacgggaggagatcctcctcgtgcacggcgccaacaagcggctcgaccccggcgtgtcgctcgccgtggtcgccatgcgcacgccggggttcagcggcgccgacctcgccaacctcatgaacgaggccgccatcctcgccggccgccgcggcaaggaccgcatcaccgtcagcgagatcgacgactccatcgaccgcatcgtcgccggcctcgagggcaccagcatgaccgacggcaagagcaagatgctcgtcgcctaccatgagatcggccacgccgtgtgcgcgtaa</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001187744.1 RefSeq:Os06g0229066]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0229066&diff=169607Os06g02290662014-05-19T02:45:38Z<p>Daweih: </p>
<hr />
<div>This gene encodes ATP-dependent zinc metalloprotease FTSH 6 (OsFtsH6) in Oryza sativa Chloroplast, homologue of AtFtsH6. It is a predicted gene base on homology comparison.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Please input function information here.<br />
<br />
===Expression===<br />
Please input expression information here.<br />
<br />
===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Please input related labs here.<br />
<br />
==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0229066|<br />
Description = Twin-arginine translocation pathway signal domain containing protein|<br />
Version = NM_001187744.1 GI:297724618 GeneID:9268843|<br />
Length = 1461 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0229066, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:6701957..6703417|<br />
CDS = 6701957..6703417|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:6701957..6703417<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:6701957..6703417<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgtcgctgacgacgacgacgagccggctgccgatctgcagggcgcagggcggcggcgtcgccaaggagaagcggacgacgccgccacctgccaagatcacgccgccttcgtcgtcgtcgtcggaggccgccggactaagcaggaggaggctgctgcaatcggccggcctcgggctcggcctcggcctaacggcggcgaggccggcgcgagccgaggcgacggcgccggaggaggtgacgtcgaacaggatgtcctactcccggttcctggagtacctcgacgccggcgccgtcaagaaggtggacttcttcgagaacggcacggtggccgtcgccgaggtggacgacgcggcggcgctgtcgagggtgcaccgcgtcaaggtgcagctcccgggcctccccgccgagctcgtccggaagctccgggacaagggcgtcgacttcgcggcgcacccggtggagcccagcgccggggtgatgctgctcgacctcctcgtcaacttcggcttccctctcctcttcgtcgcctcgctcctgtggcggtcgccgaccatgaacaaccccggcggcgggcctagcctcccgttcgggctcggcaagtccaaggccaagttccagatggagcccaaaaccggcgtcacgttcgacgacgtcgccggcgtggacgaggccaagcaggacttccaggagatcgtccagttcttgaagttcccggagaagttcacggcggtcggtgcgaggacccccaagggcgtgctgctggtcggcccgccggggacggggaagacgctgctggccaaggcgatcgccggcgaggccggcgtgcccttcttctcgctgtccggctccgagttcatcgagatgttcgtcggcgtcggcgcgtcgcgggtgcgcgacctgttcgacagggccaaggcgagcgcgccgtgcctcgtcttcatcgacgagatcgacgccgtggggcggcagcgcggcgcggggatcggcggcgggaacgacgagagggagcagacgctgaaccagctgctcaccgagatggacgggttcggcggcggcgacggcggcgtcgtcgtgatcgccgccaccaaccggccggagatcctcgacgcggcgctgctccggccggggcgcttcgaccggcgggtgtccgtcggcctccccgacgtgcgcggacgggaggagatcctcctcgtgcacggcgccaacaagcggctcgaccccggcgtgtcgctcgccgtggtcgccatgcgcacgccggggttcagcggcgccgacctcgccaacctcatgaacgaggccgccatcctcgccggccgccgcggcaaggaccgcatcaccgtcagcgagatcgacgactccatcgaccgcatcgtcgccggcctcgagggcaccagcatgaccgacggcaagagcaagatgctcgtcgcctaccatgagatcggccacgccgtgtgcgcgtaa</cdnaseq>|<br />
AA = <aaseq>MSLTTTTSRLPICRAQGGGVAKEKRTTPPPAKITPPSSSSSEAA GLSRRRLLQSAGLGLGLGLTAARPARAEATAPEEVTSNRMSYSRFLEYLDAGAVKKVD FFENGTVAVAEVDDAAALSRVHRVKVQLPGLPAELVRKLRDKGVDFAAHPVEPSAGVM LLDLLVNFGFPLLFVASLLWRSPTMNNPGGGPSLPFGLGKSKAKFQMEPKTGVTFDDV AGVDEAKQDFQEIVQFLKFPEKFTAVGARTPKGVLLVGPPGTGKTLLAKAIAGEAGVP FFSLSGSEFIEMFVGVGASRVRDLFDRAKASAPCLVFIDEIDAVGRQRGAGIGGGNDE REQTLNQLLTEMDGFGGGDGGVVVIAATNRPEILDAALLRPGRFDRRVSVGLPDVRGR EEILLVHGANKRLDPGVSLAVVAMRTPGFSGADLANLMNEAAILAGRRGKDRITVSEI DDSIDRIVAGLEGTSMTDGKSKMLVAYHEIGHAVCA</aaseq>|<br />
DNA = <dnaseqindica>1..1461#atgtcgctgacgacgacgacgagccggctgccgatctgcagggcgcagggcggcggcgtcgccaaggagaagcggacgacgccgccacctgccaagatcacgccgccttcgtcgtcgtcgtcggaggccgccggactaagcaggaggaggctgctgcaatcggccggcctcgggctcggcctcggcctaacggcggcgaggccggcgcgagccgaggcgacggcgccggaggaggtgacgtcgaacaggatgtcctactcccggttcctggagtacctcgacgccggcgccgtcaagaaggtggacttcttcgagaacggcacggtggccgtcgccgaggtggacgacgcggcggcgctgtcgagggtgcaccgcgtcaaggtgcagctcccgggcctccccgccgagctcgtccggaagctccgggacaagggcgtcgacttcgcggcgcacccggtggagcccagcgccggggtgatgctgctcgacctcctcgtcaacttcggcttccctctcctcttcgtcgcctcgctcctgtggcggtcgccgaccatgaacaaccccggcggcgggcctagcctcccgttcgggctcggcaagtccaaggccaagttccagatggagcccaaaaccggcgtcacgttcgacgacgtcgccggcgtggacgaggccaagcaggacttccaggagatcgtccagttcttgaagttcccggagaagttcacggcggtcggtgcgaggacccccaagggcgtgctgctggtcggcccgccggggacggggaagacgctgctggccaaggcgatcgccggcgaggccggcgtgcccttcttctcgctgtccggctccgagttcatcgagatgttcgtcggcgtcggcgcgtcgcgggtgcgcgacctgttcgacagggccaaggcgagcgcgccgtgcctcgtcttcatcgacgagatcgacgccgtggggcggcagcgcggcgcggggatcggcggcgggaacgacgagagggagcagacgctgaaccagctgctcaccgagatggacgggttcggcggcggcgacggcggcgtcgtcgtgatcgccgccaccaaccggccggagatcctcgacgcggcgctgctccggccggggcgcttcgaccggcgggtgtccgtcggcctccccgacgtgcgcggacgggaggagatcctcctcgtgcacggcgccaacaagcggctcgaccccggcgtgtcgctcgccgtggtcgccatgcgcacgccggggttcagcggcgccgacctcgccaacctcatgaacgaggccgccatcctcgccggccgccgcggcaaggaccgcatcaccgtcagcgagatcgacgactccatcgaccgcatcgtcgccggcctcgagggcaccagcatgaccgacggcaagagcaagatgctcgtcgcctaccatgagatcggccacgccgtgtgcgcgtaa</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001187744.1 RefSeq:Os06g0229066]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0669400&diff=169602Os06g06694002014-05-19T02:38:39Z<p>Daweih: </p>
<hr />
<div>This gene encodes FtsH protease 2 (OsFtsH2) in Oryza sativa, homologue of AtFtsH2/8.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Please input function information here.<br />
<br />
===Expression===<br />
Please input expression information here.<br />
<br />
===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Please input related labs here.<br />
<br />
==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0669400|<br />
Description = Similar to FtsH protease (VAR2) (Zinc dependent protease)|<br />
Version = NM_001064856.1 GI:115469443 GeneID:4341796|<br />
Length = 3459 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0669400, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:28601612..28605070|<br />
CDS = 28601758..28602099,28602344..28602584,28602685..28602998,28603327..28604202,28604262..28604318<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:28601612..28605070<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:28601612..28605070<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>agctcgcgcttgggaacctcggggtcgggcttcccaccttgttgggtgctaagagagtatctagacaaggacagagtgaagaaggttgatttgtttgagaatggcacgattgccattgtagaggctatttctcctgagctcggcaaccgtgtgcagagagttcgtgtgcagcttcctggtctaagccaggagcttctccagaagttgagggaaaagaatatcgattttgctgcacacagcaaccaggaggactctggttctcttctgttcaacctgatcggaaacttggctttcccacttatccttattggtggtctatttttgctgtcaagaagagcacaaggtggccttggtggacctaacggtcctggctttccccttggttttggtcaatctagggccaagtttcagatggaacccaacactggtgttacatttgatgatgttgctggtgtcgatgaagcaaagcaagacttcatggaagtggttgagttcttgaagaaaccagaaaggttcaccgctgttggtgctcgcatccctaagggtgttctgcttgttggtcctcctggaactggtaagactttgcttgccaaggctattgcaggggaagctggtgtgccgtttttctcgatatcaggatctgagtttgtggagatgtttgtcggagttggtgcctcccgggttcgtgatctcttcaagaaggcaaaggagaatgctccttgcatagtatttgttgatgaaattgatgctgttgggaggcaaagagggacaggtattggtggtggaaatgatgaaagggagcagactttgaatcagttgctgactgagatggatggttttgaggggaacactggaatcattgttattgctgccaccaacagggctgatatcctggattctgctttactcagacctggacgttttgacagacaggtaagtgttgatgttcctgatgtacgcggaaggacagagattcttaaggtgcacggtagcaacaagaagttcgatactgatgtctctcttgaagtcatagcaatgagaacacctggctttagtggagcagacttggcaaatcttctaaatgaagcagccatattagcgggccgacgtggtcggacagcaatttcctcaaaggagattgatgattcaattgacagaatagtggctggtatggaaggaactgtgatgacagatgggaagagcaaaagtcttgttgcttaccatgaagttgggcatgcaatttgtggaactttgacgcctggccacgaccctgtccaaaaggttactttgatcccgaggggtcaagctcgtggtctcacatggttcattccaatggacgacccaacgctcatctccaggcaacaactgttcgccagaattgttggtggccttggcggtcgtgctgctgaggagatcatttttggggagcctgaggtgaccacaggagctgctggtgacttgcagcaaattactggcctagccaagcagatggtggttacattcggtatgtcggacattggtccgtggtcgctaatggactcaggagctcagagcggcgatgtcatcatgaggatgatggcaaggaactccatgtcggagaagctcgcggaggacatcgataccgctgtgaagcggttgtccgatgaggcctacgagatcgctttgagccagatcaggagcaaccgggaggccatggacaagatcgtggaggtgctccttgagaaggagacattgagtggtgacgagttcagggcgattctctccgagttcacggagatccctgtcgagaaccgggttcccccagccacgccggcagctctccctgcctaa</cdnaseq>|<br />
AA = <aaseq>SSRLGTSGSGFPPCWVLREYLDKDRVKKVDLFENGTIAIVEAIS PELGNRVQRVRVQLPGLSQELLQKLREKNIDFAAHSNQEDSGSLLFNLIGNLAFPLIL IGGLFLLSRRAQGGLGGPNGPGFPLGFGQSRAKFQMEPNTGVTFDDVAGVDEAKQDFM EVVEFLKKPERFTAVGARIPKGVLLVGPPGTGKTLLAKAIAGEAGVPFFSISGSEFVE MFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGTGIGGGNDEREQTLNQLLTE MDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVSVDVPDVRGRTEILKVHGSNKK FDTDVSLEVIAMRTPGFSGADLANLLNEAAILAGRRGRTAISSKEIDDSIDRIVAGME GTVMTDGKSKSLVAYHEVGHAICGTLTPGHDPVQKVTLIPRGQARGLTWFIPMDDPTL ISRQQLFARIVGGLGGRAAEEIIFGEPEVTTGAAGDLQQITGLAKQMVVTFGMSDIGP WSLMDSGAQSGDVIMRMMARNSMSEKLAEDIDTAVKRLSDEAYEIALSQIRSNREAMD KIVEVLLEKETLSGDEFRAILSEFTEIPVENRVPPATPAALPA</aaseq>|<br />
DNA = <dnaseqindica>2972..3313#2487..2727#2073..2386#869..1744#753..809#agttcggcccaccccccccccctctcccctccacgccccgctccgatccactcaggtgcgtgctcgtttgctttggattcagtgcttccctttacgatttcgcttgcgtgcgtgggtgcgtgcgtctggttctgtttctagcttgaatccagtgtgtaagtgtgtggcgtttggtctctgatgcgatgcggatgttctgtttgttagccagtatgcttttcggaatttggagtactggtctagctttttactggtaaagatggtgggactgagatcgttagcgtgttcgttccaatcgataggttaatcagcaagtagaagctacggtgagctactgaattcggatttcatactggtttggggatgatggttgtagctgtttgtagttcgagaagggattacaatagcttctacttgtaggggtgattgtgaagagatgctttacctttgttcttgaatacttacggtaggacagcttgttagttacagctaagttggattgatatgaagtgattagcttagttgaagtatcggatgaagcaatttctgccatacaacacttgtatgattgacaagatctccctttcattacttttaacagtgagcgaagatggctccaacatccatgagccttgctgcaaagacgccgcttcccttctcgacacttccttcgagtggcgttgcacagaggccagtttctgtgactgcctccctcgagcacaagactaatgatgccagaaggaagttcctaaagctcgcgcttgggaacctcggggtcgggcttcccaccttgttgggtgctaagagagctcttgctgaagagcaaggtgtctcgtcgtcgagaatgtcttactcgaggttccttgagtatctagacaaggacagagtgaagaaggttgatttgtttgagaatggcacgattgccattgtagaggctatttctcctgagctcggcaaccgtgtgcagagagttcgtgtgcagcttcctggtctaagccaggagcttctccagaagttgagggaaaagaatatcgattttgctgcacacagcaaccaggaggactctggttctcttctgttcaacctgatcggaaacttggctttcccacttatccttattggtggtctatttttgctgtcaagaagagcacaaggtggccttggtggacctaacggtcctggctttccccttggttttggtcaatctagggccaagtttcagatggaacccaacactggtgttacatttgatgatgttgctggtgtcgatgaagcaaagcaagacttcatggaagtggttgagttcttgaagaaaccagaaaggttcaccgctgttggtgctcgcatccctaagggtgttctgcttgttggtcctcctggaactggtaagactttgcttgccaaggctattgcaggggaagctggtgtgccgtttttctcgatatcaggatctgagtttgtggagatgtttgtcggagttggtgcctcccgggttcgtgatctcttcaagaaggcaaaggagaatgctccttgcatagtatttgttgatgaaattgatgctgttgggaggcaaagagggacaggtattggtggtggaaatgatgaaagggagcagactttgaatcagttgctgactgagatggatggttttgaggggaacactggaatcattgttattgctgccaccaacagggctgatatcctggattctgctttactcagacctggacgttttgacagacaggttagtttttatccactgaacattcttttggatgtgaacatctgttgtattctagtttttaactcattcaattgtgacacaattaagtaaaaatgtttcagctatagcgtttacctttgttctgtattattgaagttctcacgtggttttaggctagaagtccaataaatactcgttcttggttgtagcttactcacgcaatcagtttggatgcttttggtttcgagcttgagttatctttgagtgttctcaaagttagaattttgttatcctcatatgtccaaaaccttgctaacattatggatataccttttaacttgttgcacaggtaagtgttgatgttcctgatgtacgcggaaggacagagattcttaaggtgcacggtagcaacaagaagttcgatactgatgtctctcttgaagtcatagcaatgagaacacctggctttagtggagcagacttggcaaatcttctaaatgaagcagccatattagcgggccgacgtggtcggacagcaatttcctcaaaggagattgatgattcaattgacagaatagtggctggtatggaaggaactgtgatgacagatgggaagagcaaaagtcttgttgcttaccatgaagttgggcatgcaatttgtgggtaagagataatctgcatatttgctaactgtgcttttgtttattacttgcatatcaaaattccgtgcaactattcatatctggaaatgtattctttgtagaactttgacgcctggccacgaccctgtccaaaaggttactttgatcccgaggggtcaagctcgtggtctcacatggttcattccaatggacgacccaacgctcatctccaggcaacaactgttcgccagaattgttggtggccttggcggtcgtgctgctgaggagatcatttttggggagcctgaggtgaccacaggagctgctggtgacttgcagcaaattactggcctagccaagcaggtacgctatatttctattctagaactttgctaattgatctacagaaactttgaagcagtagactatgtaatctgttctgcttctgcaccttttatgacttacaaaccagtctggaggataaatttgctgcaaaataacatgctagaaaaactgacagcatttacatatatattaaacatctgttcttagtccttcccctttctttcttttgctaattacacctggggtcacaatgcttctgcagatggtggttacattcggtatgtcggacattggtccgtggtcgctaatggactcaggagctcagagcggcgatgtcatcatgaggatgatggcaaggaactccatgtcggagaagctcgcggaggacatcgataccgctgtgaagcggttgtccgatgaggcctacgagatcgctttgagccagatcaggagcaaccgggaggccatggacaagatcgtggaggtgctccttgagaaggagacattgagtggtgacgagttcagggcgattctctccgagttcacggagatccctgtcgagaaccgggttcccccagccacgccggcagctctccctgcctaaatcccactagttttgtaggaatggaacatgctgtaagatgttcatattttcgattcttggtgtacactaactttactttgccggccggattttttggcatactacatgtaacttactcttggttaattataaaatgtaattagctg</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064856.1 RefSeq:Os06g0669400]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0725900&diff=169600Os06g07259002014-05-19T02:27:02Z<p>Daweih: </p>
<hr />
<div>This gene encodes FtsH protease 1 (OsFtsH1) in Oryza sativa, homologue of AtFtsH1/5.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Please input function information here.<br />
<br />
===Expression===<br />
Please input expression information here.<br />
<br />
===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Please input related labs here.<br />
<br />
==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0725900|<br />
Description = Similar to Cell division protein ftsH homolog, chloroplast precursor (EC 3.4.24.-) (DS9)|<br />
Version = NM_001065160.1 GI:115470051 GeneID:4342113|<br />
Length = 7643 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0725900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31747348..31754990|<br />
CDS = 31747627..31747848,31752229..31752400,31752490..31752673,31752768..31753014,31753720..31754955<br>|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31747348..31754990<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31747348..31754990<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggcgccgccgtgctccatctcctccgcctcccacctcctcatcactgcctccctccccaagccctccctccgcccacctcgcctcccccaccccaagcccctccccgccgccctcctcgccctcgccgccgccgcccccacgctccccgccctcgccgacgtgcccgcgccacccccctccccgacccaggatgtccaggtactagaggccccctccccggccgccaaccctttctccaacgccctcctcaccgcgcctaagcccacctcctccgccgccgccgacctccccgagggcgcccagtggcgctacagcgagttcctcagcgccgtcaagaagggcaaggtcgagcgcgtcaggttctccaaggatggtggcctactccagctcaccgccatcgacggccgccgcgccaccgtcgtcgtccccaacgaccccgatctcattgacatcctcgccaccaacggcgtcgacatctccgtcgccgagggcgacgccgccggccccggcggattcctcgccttcgtcggcaacctcctcttccccttcctcgctttcgctggcctcttcttcctcttccgccgcgcccagggcggccccggcgctggacctggcggcctcggcgggcccatggacttcggccgctccaagagcaagttccaggaggtgcccgagactggcgtcaccttcgtcgacgtcgccggcgccgaccaggccaagctcgagctgcaggaggtcgtcgatttcctcaagaaccccgacaagtacaccgcgctcggggccaagatccccaagggatgcctgctcgtcggcccgcccggaaccgggaagacactgctggcgcgtgccgtcgccggggaggccggagtgccattcttctcctgcgccgcgtccgagttcgttgagctgttcgtcggtgtcggtgcctccagggtcagggacctgttcgagaaggccaaggccaaggccccttgcatcgtcttcattgatgaaattgatgccgtcgggaggcagcgtggtgctgggctcggagggggaaatgatgagagggagcagaccattaaccagctcttgactgagatggatggatttgccggcaatagcggtgtcattgtgctcgccgcgacaaacaggcctgatgtgctcgacgcggctcttcttcgccctgggaggttcgatcggcaggtcaccgtagacaggccggatgtcgccgggcgtgtcaagattcttgaggttcactcgagaggaaaggctctggcaaaagatgtcgactttgagaaaattgcccggagaacccctggtttcactggagctgatcttcaaaacttgatgaatgaggctgctattcttgctgctcgtcgtgatctgaaggagattagcaaggatgagatctctgatgctttggagaggatcattgctgggcctgagaagaaaaatgctgttgtttcagaagagaagaggagacttgtggcttaccatgaggctggtcatgcccttgttggtgccctcatgcctgaatatgaccctgttgctaagatttccatcattcctcgtggtcaagctggtggactcacattctttgctccaagcgaggagaggcttgagtctggattgtacagcaggagctacctagagaaccaaatggccgtagctcttggtggaagggttgccgaagaggtgatctttggccaagaaaatgtgacaactggggcctccaacgattttatgcaggtttcacgtgttgcaagacaaatggttgaaagatttggattcagcaagaagatcgggcaagttgcgattggtggacctggtggaaatcctttcttgggccagcagatgtcgagccagaaggactactcgatggccaccgcggatgttgtggatgctgaggtgagggagctcgtggagaaggcctactcaagagcaacgcagatcatcacgacacacatcgacatcctccacaagcttgctcagcttctgatggagaaggagacggtggacggggaagagttcatgagcctgttcatcgacggtcaggcagagttgtttgtcgcttaa</cdnaseq>|<br />
AA = <aaseq>MAPPCSISSASHLLITASLPKPSLRPPRLPHPKPLPAALLALAA AAPTLPALADVPAPPPSPTQDVQVLEAPSPAANPFSNALLTAPKPTSSAAADLPEGAQ WRYSEFLSAVKKGKVERVRFSKDGGLLQLTAIDGRRATVVVPNDPDLIDILATNGVDI SVAEGDAAGPGGFLAFVGNLLFPFLAFAGLFFLFRRAQGGPGAGPGGLGGPMDFGRSK SKFQEVPETGVTFVDVAGADQAKLELQEVVDFLKNPDKYTALGAKIPKGCLLVGPPGT GKTLLARAVAGEAGVPFFSCAASEFVELFVGVGASRVRDLFEKAKAKAPCIVFIDEID AVGRQRGAGLGGGNDEREQTINQLLTEMDGFAGNSGVIVLAATNRPDVLDAALLRPGR FDRQVTVDRPDVAGRVKILEVHSRGKALAKDVDFEKIARRTPGFTGADLQNLMNEAAI LAARRDLKEISKDEISDALERIIAGPEKKNAVVSEEKRRLVAYHEAGHALVGALMPEY DPVAKISIIPRGQAGGLTFFAPSEERLESGLYSRSYLENQMAVALGGRVAEEVIFGQE NVTTGASNDFMQVSRVARQMVERFGFSKKIGQVAIGGPGGNPFLGQQMSSQKDYSMAT ADVVDAEVRELVEKAYSRATQIITTHIDILHKLAQLLMEKETVDGEEFMSLFIDGQAE LFVA</aaseq>|<br />
DNA = <dnaseqindica>7143..7364#2591..2762#2318..2501#1977..2223#36..1271#tctccacccccaccttcgcccacctcgcatcgaccatggcgccgccgtgctccatctcctccgcctcccacctcctcatcactgcctccctccccaagccctccctccgcccacctcgcctcccccaccccaagcccctccccgccgccctcctcgccctcgccgccgccgcccccacgctccccgccctcgccgacgtgcccgcgccacccccctccccgacccaggatgtccaggtactagaggccccctccccggccgccaaccctttctccaacgccctcctcaccgcgcctaagcccacctcctccgccgccgccgacctccccgagggcgcccagtggcgctacagcgagttcctcagcgccgtcaagaagggcaaggtcgagcgcgtcaggttctccaaggatggtggcctactccagctcaccgccatcgacggccgccgcgccaccgtcgtcgtccccaacgaccccgatctcattgacatcctcgccaccaacggcgtcgacatctccgtcgccgagggcgacgccgccggccccggcggattcctcgccttcgtcggcaacctcctcttccccttcctcgctttcgctggcctcttcttcctcttccgccgcgcccagggcggccccggcgctggacctggcggcctcggcgggcccatggacttcggccgctccaagagcaagttccaggaggtgcccgagactggcgtcaccttcgtcgacgtcgccggcgccgaccaggccaagctcgagctgcaggaggtcgtcgatttcctcaagaaccccgacaagtacaccgcgctcggggccaagatccccaagggatgcctgctcgtcggcccgcccggaaccgggaagacactgctggcgcgtgccgtcgccggggaggccggagtgccattcttctcctgcgccgcgtccgagttcgttgagctgttcgtcggtgtcggtgcctccagggtcagggacctgttcgagaaggccaaggccaaggccccttgcatcgtcttcattgatgaaattgatgccgtcgggaggcagcgtggtgctgggctcggagggggaaatgatgagagggagcagaccattaaccagctcttgactgagatggatggatttgccggcaatagcggtgtcattgtgctcgccgcgacaaacaggcctgatgtgctcgacgcggctcttcttcgccctgggaggttcgatcggcaggtcaccgtagacaggccggatgtcgccgggcgtgtcaagattcttgaggtgagcacttgcctattgtttagctagcttgtgatttcctatgcttttagtagatattgttctgaatttcaatgaaccaattcacaaatttgcttagttcagtgccattgatgataatttaattggcattcttatagtagctttgtccatttagtcgaaattactaattgcttagaacatgaagaacttaactaaaaatcaaactttgcctgagctgactcaaagatggaccattgttcatgtatcatagcatatgttgttatacagctgaccatggcatgattttgctctgcatcatagttcaagatattatgtacagttactttaaccgtagaatgatcttattgtggtctgcatatctaatcaggagcagctgtactcatttgcagaaatttatgtggttgatatatatgttagctcatatcaatccgtatgggtactttgcaatttctgtttatgtagtccattccatcgtcctaagagggatgctggttttgcagtgctacttcatctagtctttgatgctccttaacttagggatttcaatgtatttccatctactgctaattttctttgaagaagctatgctgagatacatagagtgcctttctgaatgctgatcgacttttccttatggcttcccaatttgataagcttttatctgtaccaaagcgcaagtttacaagacatgttattgtctgataggttcactcgagaggaaaggctctggcaaaagatgtcgactttgagaaaattgcccggagaacccctggtttcactggagctgatcttcaaaacttgatgaatgaggctgctattcttgctgctcgtcgtgatctgaaggagattagcaaggatgagatctctgatgctttggagaggatcattgctgggcctgagaagaaaaatgctgttgtttcagaagagaagaggagacttgtggcttaccatggtaaatcttgtcaccatattaacttgtgacttgagctgaaattatcctggtgtgcagaggttcctaatccatttttgaacttttcaatttgcagaggctggtcatgcccttgttggtgccctcatgcctgaatatgaccctgttgctaagatttccatcattcctcgtggtcaagctggtggactcacattctttgctccaagcgaggagaggcttgagtctggattgtacagcaggagctacctagagaaccaaatggccgtagctcttggtggaaggtgggttttagtgaaatgttcatatctgcgtacatgtgctattgttgttgtgcaaagtcttgaactgtgtgagcttacatggtttgcagggttgccgaagaggtgatctttggccaagaaaatgtgacaactggggcctccaacgattttatgcaggtttcacgtgttgcaagacaaatggttgaaagatttggattcagcaagaagatcgggcaagttgcgattggtggacctggtggaaatcctttcttgggccagcaggtaaatcactataactgaaatagactatcaaagaagaaaaacttcttaccctagaagcagagtctgtgaaaattaactgaagaactagacgtgcaaacttcaacaatgacaacatatgagcccagcctttcttgccctagtgtgcatctagtgttctgaagtgtctactgtcctatggggaaggtccatttcctgaagttcagtttgttcctttaaccttgatgaatagtatagtctagtctttcctgtaactttgtgtcacgccccgaactagtcccgaccggaactagcccgtgacgctccaaattaacctgttaatcgataccagtcctaggaaacagtgctggtatcacagggagacagagtatcacagcaacagaggtctctttattatagagtagaagtacagtcatgttgggctgcggacagatcccgagctcacaactgcattacaaaaggaaagcggaagccaggacttggaccaatcatcacaggcgcgacttgggaactaggccgaaaccctaaaactcatcgtagccgacttgctcctggaagaactcctcgtcagcaggatccacttcatcttcttcagcaactggggggggggggggattatttatatagagcaagggtgagtacgggagtactcagcaagccaggggaaaataagtgtttaatgcaggcttcaaggaaaggctgttgtttttgcaattgattttatttgaactctttttctaaaacaactaggtgagtgcttctcaaacgacacggatgagacagtgcgtctcgtccggtcggagtatgtgcaatgtgtcagtctttaggattgcaacaaggttggcacccggccaacagcttttcaaacggccacccgggccaacaacttttcaaacggccacccgggcctagctgatctcatcagctgcagatttttcaaacatcgaaccccttttcacaacagcaatttcacaagcagtagtcaaacaaaactacgctaggaatcacctcacatccgcccatgaccgtgggcacggctgttcgaacagtttaatgacctctgcagagggggtacactttacccacatgacattactaacccggatcatccagcccgtggggatcagccacgtcgggagacctccaagctttcatgacaaggcatttcgaaagccgacacaggtttaccatatgccgacgagaggggtcccagaccaacaacaggttaggtcccagaccatactgtgccaggaagcccaggggtcctccccgacaccaccccggcgaatccacatgtctctcggcatcaaggctcccccgattagcaatttactcagccaggggcatcccattccacccatgtggtcgtacttgtcttatgttcggatgaaattccaaggaaacggtccttaagtgcaagagcgggaaaccgtacacccggtacgttccccggtccgcggttttggaaattcatttagttcgcaagcaccgacccaggtgtcgggttttccaagtcttttgtaaacctaagttttacccaagttgtttttcagattttaagtttgaaggcgtccgtcgatactcgcgcaggatgcacggatatcgagacgcaactagatggttacaaggggacatgatataacaattaacaaagggggggatctaatgcagcaaattaggtaagtccgccaatctgccttgcagacgggacaacggattaagtgtgatcctatcagtgcataatatttttcaagcaacataattaaatttcaaatataggctcaagatgttcaaaggtggcttgccttgctcgagatctggagcttgatcctcgaaatcctcgcactgcgggtcttcgggctctgaaactatacgcgaaacggaacaactcaacaaacggcgaaaataaagccctattaatgacctctaagcgtgccattagatagatctcgagatttgaggaattttggaagttgaacggagtcaaacggatttacggttgggaagatattgaatttctaaaattattgaatttttgatctaaaggaaaaggatttaattaaatcctttttggaaaagaaaaagaaaagaagggagggagggaatatagacttttctcggacggctagggcgcggcccaagagaaatggagcggtccggccgagctaaatgggccggccggcccaaggcggcccaaactcgcgcgcgcgcgggagggaggagagagagagccggtggaccgggctcaccacgcgcggtcccgggtgggacccgcttgtcagcggctcggctcaccgtgcggagggagcacgcgacgcacgcgcgcgggcggggaagggacgcggtgcatgcgcgcggtccgcggtggacgcaggtgcggcgggcccacgcgcagcctcacggctcgcggtggatcgcgcgcacgcggagggaccgaccggggtcggcccgacccggtctggctgagctggcgccgacgtggcgcctacgtggctgccacgcgggccggcgggaggtagacgacgacccggccgcgaatggacggcgggcggcggcggcgagcggcggagcgaaccacggcgatacgggcaacagcgagcacaccggacggttgcacgtgacaaggggaggcgagccaacggctcggattcgccggaggttgctcgacggcggcggattgcggcggcggcaaccggcggcgggagaagagggaaacggcgacgaggtcacgagaggtcgattcccggcggtgagagcatctacgcggcttcgggaactcgacgctagcgtcggattgggcggaactacgccgagcgaggccggcgacgagcgggtgctacggagctcgggcggcgacggcggcgagcacacggcgagcgacggcaacggtcggggcggcgccggctaactacggggagtctactcaagctactaccgaaagctagggggaggagatggagcgaggaggaagaacggagagagacactaccgtgcgggacggggcgcagtgacgagaggccgacggcgcgggagtgatctctccggcctcgggccgaggaagaggaagaagagggcgcgcccggagtccccttccgcgtccacgaacgcaccggctctcccggcgcttggcgatggacggcggaggcgaggcagagcacgggagcggcggcaacggtgaggaggcgaatgggagaggaaaggaagggagggggaagacgggtttaaatgggcggcaatgtcggtttgagagaggggaaccgacatgggcggtcaagccgacgagctggcgctccggctagcgtccaaagcggcgacaggaggtgacgccggcgagaggggaaaaaggggagaaaaagaggaaaaggagggagagaaagggggctcgcccctttgccgattcgggaaaaggaggagggagcggggagactcggcagggagagaggggctctctgcctccgccccttggaggctagcgcgcggagtggcgggggccgggcgatgacgacggcgatgacggtgggatggtttggagcggcgcgacgacacgggcgacaggcgcgggctggcgcggcagggggggggtgacggcggcggcgaccaggcggtcggccactcggcacgcgcgcgcgggaagcaacgggcggcgcggctgggcagcgggaccggcgacgcggacggcgcagacaacgcggctcgggcgggaaaccacgcgggcgcgcgcgcgagcaacggcgcgcggggccggctttgcgagcggggagctcgcgcgagaggagggggcgctcggcgcggctctcgcacgcgcgagcagcgagcggggggaaaaacgcgccggggtggggagggagcgagagagagagagagagcgcccgggagagagggggccggggaggagatgggccgagcggaattcggcccatcgaacctgggggaggcaaactagacttttgcggagggattcgatttggaaggatttggattcggaattgaactcgacgatagatcggggattgagatcttgagatggcacggacactagacaacaatcaaagaaacaaatttcgcaattagggtttttcggagataattttcccgctaggcgccacgacggaacgggcgctacactttgccactcacatagtcatatactgccaatgttgtcatcatcaacacacttggtctataaaagcagtgacaaaatcaaagagtagtgccagttaggcacacaactcttttgtgtggctgtgagtagattactggttgccctgccagacgtggcaatctgtctaacctgcgttggttttgcttctcggaatttctatcggcaattcgtagtatctgtgtgaatggtagcatgatatttttcatatcctttggttgcagatgtcgagccagaaggactactcgatggccaccgcggatgttgtggatgctgaggtgagggagctcgtggagaaggcctactcaagagcaacgcagatcatcacgacacacatcgacatcctccacaagcttgctcagcttctgatggagaaggagacggtggacggggaagagttcatgagcctgttcatcgacggtcaggcagagttgtttgtcgcttaattgcgttgtatgttcatccccttgggtgaaatggagaaggcgaatttgtaggaagcacatcaatttaggcgagatgtttttgcatatacttgtatagttcattgtaaagaatggcaaatactgacattgatcaagtaaacagttggtgcttacgattatagcaccgctaactaaacatgattccactgtgctcgctgtacatctgcactgaagacatgtcgcagttgcaggcttgcagctatttcatccgtgtggactttaatatgtttgaatccagat</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065160.1 RefSeq:Os06g0725900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0842600&diff=169482Os01g08426002014-05-18T09:34:41Z<p>Daweih: </p>
<hr />
<div>This gene encodes FtsH protease 3 (OsFtsH3), homologue of AtFtsH3/10.<br />
==Annotated Information==<br />
===Function===<br />
Please input function information here.<br />
<br />
===Expression===<br />
Please input expression information here.<br />
<br />
===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Please input related labs here.<br />
<br />
==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0842600|<br />
Description = Similar to AAA-metalloprotease FtsH|<br />
Version = NM_001051309.1 GI:115440988 GeneID:4327502|<br />
Length = 5416 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0842600, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:37938310..37943725|<br />
CDS = 37938596..37939060,37939135..37939362,37939901..37940668,37940838..37941320,37942471..37942598<br>,37943127..37943195,37943276..37943480,37943593..37943655|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:37938310..37943725<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:37938310..37943725<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgagcctctcctccctctcccgcgccctcgcccggtccgcgcgctcctcccggcagcgccagggttctctgctcggggggcatggcgggctccgtgcttcgtcgccgccgctgccctgcggcgagttgggttttctgcgcagctatgtgacgtcagttatcgggaatcgggcagcggtggcctccggggcagggaagggtggggactggaggttcctgcttgctagccggcagttccggcggctattctccgacaagtcgaagaaaaaccacgggaagcattcggaggaggagaataaaggtaaaggggatgagagtgacaagtccgactcaaagaagcaatccagttcaggtgatcaatggaatttcgaggagagcattaaacaattcaaggacatgattgctcctttattcctttttggcctattattgttatctgcatcagcatcttcatcagagcaggagataagcttccaagaattcaagaataagttgttggaacctggtttagttgaccacattgttgtttcaaacaagtcaattgctaaggtttacgtcaggagttcaccttcaattgatcgaattcaggatagtgatatccatataactacaagtcatcttcctgggatagagtctcctagcagctacaagtattacttcaatattggaagtgttgactcctttgaagagaagttacaagaggcccagaaagcattggagatagatccacactattatgtcccaatcacttacactactgaagcaaaatggtttgaggaagtaatgaagtatgtcccaaccgttctaattattggattaatatatttgcttgggaaaaggatacaaaatggatttactgttgggggtggtcctgggaaaggaggccgtagcattttcagtattggaaaagttcaagtgacaaaattggataaaaactccaaaaataaggtgttctttaaggatgtagctggctgtgatgaagccaaacaagaaattatggaatttgttcatttcctgaaaaatcccaagaaatatgaagaactgggagcaaaaataccaaagggtgctcttcttgttggtcccccaggcacaggaaagactctccttgctaaagctactgcaggagagtctggtgtgcccttcttgtctatttctggttcagacttcatggaaatgtttgttggtgttggaccatccagggtgcggaacttatttcaagaagctcggcagtgttcacctagtattgtattcattgatgaaattgatgcaatcggccgtgctagaggccgtggaggtttttctggtggacatgatgagcgtgaaagcacattgaaccagttacttgtagagatggatgggtttggaactacatctggtgttgttgttcttgctggtaccaataggcctgacatcctggataaggctttgctaagaccaggaagatttgatcgtcagataagtattgataaaccagacataaagggtcgtgatcaaatatttcgcatctaccttaaaaagctgaaactggacaaggaaccatcattttattcacaaagattagctgctttgacacctggatttgctggagctgacattgccaatgtttgtaatgaggcagctttaattgctgcaagaagtgagggcacactcattacaatgcaacattttgaatctgcaattgatagggttatcggaggcttggaaaagaaaaacaaggtgattagcaagttggagcggcgaactgttgcttaccatgaatctggtcatgctgttgctggatggtttttggagcatgccgagccactactgaaggtcacaattgttcctcgtggaacagctgctttaggttttgctcagtatgtgccaaatgacaatcttttgatgactaaagagcagctttttgacatgacctgcatgacactaggtggccgagctgctgaggaggtcttgattggaaagatctcgactggtgcacagaatgatctggagaaagttactaagatgacttatgcccaggttgcagtatatggcttcagcgaaaaggttggccttctttctttccctcaaagggaggatggttttgagatgagcaaaccatatagcagccagacggcatccatcattgacactgaggtgagggaatgggtcgctaaggcctatgaaaaaactgttgagcttataaaacaacataaagatcaagttgcacaaatcgccgagctgctgcttgagaaagaggttctccaccaggatgatcttgtccaggttctaggggaacgcccattcaagacactcgagccaactaattatgatcgattcaaacaaggttttcaagatgaagacagcaataggaatgctgaattgtcaaatgcagatggagcatcatcccttggagaggctgtggcatcataa</cdnaseq>|<br />
AA = <aaseq>MSLSSLSRALARSARSSRQRQGSLLGGHGGLRASSPPLPCGELG FLRSYVTSVIGNRAAVASGAGKGGDWRFLLASRQFRRLFSDKSKKNHGKHSEEENKGK GDESDKSDSKKQSSSGDQWNFEESIKQFKDMIAPLFLFGLLLLSASASSSEQEISFQE FKNKLLEPGLVDHIVVSNKSIAKVYVRSSPSIDRIQDSDIHITTSHLPGIESPSSYKY YFNIGSVDSFEEKLQEAQKALEIDPHYYVPITYTTEAKWFEEVMKYVPTVLIIGLIYL LGKRIQNGFTVGGGPGKGGRSIFSIGKVQVTKLDKNSKNKVFFKDVAGCDEAKQEIME FVHFLKNPKKYEELGAKIPKGALLVGPPGTGKTLLAKATAGESGVPFLSISGSDFMEM FVGVGPSRVRNLFQEARQCSPSIVFIDEIDAIGRARGRGGFSGGHDERESTLNQLLVE MDGFGTTSGVVVLAGTNRPDILDKALLRPGRFDRQISIDKPDIKGRDQIFRIYLKKLK LDKEPSFYSQRLAALTPGFAGADIANVCNEAALIAARSEGTLITMQHFESAIDRVIGG LEKKNKVISKLERRTVAYHESGHAVAGWFLEHAEPLLKVTIVPRGTAALGFAQYVPND NLLMTKEQLFDMTCMTLGGRAAEEVLIGKISTGAQNDLEKVTKMTYAQVAVYGFSEKV GLLSFPQREDGFEMSKPYSSQTASIIDTEVREWVAKAYEKTVELIKQHKDQVAQIAEL LLEKEVLHQDDLVQVLGERPFKTLEPTNYDRFKQGFQDEDSNRNAELSNADGASSLGE AVAS</aaseq>|<br />
DNA = <dnaseqindica>4666..5130#4364..4591#3058..3825#2406..2888#1128..1255#531..599#246..450#71..133#gggatcaaaaccctaatacggcccgcccccgcctcgatctccgtccaatgcgctcgccgccgccgccgccatgagcctctcctccctctcccgcgccctcgcccggtccgcgcgctcctcccggcagcgccaggtgagtgcccgagccgcaccccctccccttcccctctccgattggtgtggcttcttcctgcgcgtcctcgccctaatggctcgggcggcgtgtgcggatgtcgttgccgtagggttctctgctcggggggcatggcgggctccgtgcttcgtcgccgccgctgccctgcggcgagttgggttttctgcgcagctatgtgacgtcagttatcgggaatcgggcagcggtggcctccggggcagggaagggtggggactggaggttcctgcttgctagccggcagttccggcggctattctccgacaagtcgaagaaaagtaagctttctggctggttaggtggtttttggggatatctggtgactctgttaatatttaacgttgccttttttattcagaccacgggaagcattcggaggaggagaataaaggtaaaggggatgagagtgacaagtccgactcaaagagtaagtgcttattttaacctgcattcctcgctcttgtgttggatatggtttattaaggtgttgagctttgcaaaatattttgaaagagagtagacttttacataccagtggcgacttaaccttccgagtgctgccctaaaatcatgctatatcataacttgtgatttctagttttagtatactgataatagaaaacaatgttaagaagatgatatgcagagcttttgtaagtggcaatacatgttaaacagagtggggctagttgatgttttattttattgcacaaatacccctaacagtatgtaagtatcatgtatagctgaaaatgctaatatttgagttttagtgcctcataaactgcccgctcacctttaagggagaggtactagcatgagtattgtggaatacgacaacatgagcctttagcccaaagcaagttggttgcgtacaatttgtaaagtttgatgcatccgtatatagcattatactgcaaaccacatacttaatgtggtcatttctggtgtgcagagcaatccagttcaggtgatcaatggaatttcgaggagagcattaaacaattcaaggacatgattgctcctttattcctttttggcctattattgttatctgcatcagcatcttcatcagagcaggaggtctgttcctactgaaaaaccgactgttaaacatgagcaacagtagtggcttgtgtgtttcattgtcattttacctgttcacacttgtggatagaagatagctgttatcaaaatccccatttactttactgaaggtcctcgcctttggtcagccttataaatttcagaaaagaatcattccaattctgtgtggtatataaattttgtcagtgttcattttgctgcagtccaattagagaggtgatatgaggttatatataaggttactttttttatcttgaaaaaaatatttggtggtttcgctggcctagcaaagtggtaaggaaaaaaaaattatgctttcacatgtagtccttagtttatgttttatttgtacataacacaattgatgataattaattagctgcccccctccattcctaaataattttcactatactattcatatcctaagtttgacttctcatattttatcaagaaacttgtaaatatttaaaagtatactccctccatccctagatgtaagggattctagctatgaatctggacacgtgcatgtccagatccatatatttatagccagaattccttaaatttagagacagagggagtactatattatgaaattatattacatgctaaatatgatcatactgttttcattcatcaaaacatgatatatatatatatttttaaaaaagttgtggtcaaagttgtaacagcaccggttaatggtgacaagcaaatgtaaacagagggagtatgactgaagttatggttgactgaaaagatttaaatcacatagttgtactgatggagatggtatgcttttgctcgagagcattaatgtgattatttggtgcagcatgtgaccctagctacagtacttcattactgatggcatatcatatcctatcctaagagtttgatatagggcattttagcatgagcttcgtatgttgttagtgccatgttggtatgaaatgcaatatggttggtattctacatcctattaatcatctaatcctgtataactatatcatatatccacacttcagttccctaccagtttctttttctgtgcgacacatacattcatttcctagtccattataacttgtgatacccacttaattgtaattcttttcagataagcttccaagaattcaagaataagttgttggaacctggtttagttgaccacattgttgtttcaaacaagtcaattgctaaggtttacgtcaggagttcaccttcaattgatcgaattcaggatagtgatatccatataactacaagtcatcttcctgggatagagtctcctagcagctacaagtattacttcaatattggaagtgttgactcctttgaagagaagttacaagaggcccagaaagcattggagatagatccacactattatgtcccaatcacttacactactgaagcaaaatggtttgaggaagtaatgaagtatgtcccaaccgttctaattattggattaatatatttgcttgggaaaaggatacaaaatggatttactgttgggggtggtcctgggaaaggaggccgtagcattttcagtattggaaaagttcaagtgacaaaattggataaaaactccaaaaataaggttagtttttatttgctatttccatctcttgctattttgaacaaaacatgaaattcatatccttttttgtaaatgtttgttctgtagctttattcaccattgacaaagaacatacaataacatattcatataattctactgaagtaaccttgtttcttgtgtgctacaggtgttctttaaggatgtagctggctgtgatgaagccaaacaagaaattatggaatttgttcatttcctgaaaaatcccaagaaatatgaagaactgggagcaaaaataccaaagggtgctcttcttgttggtcccccaggcacaggaaagactctccttgctaaagctactgcaggagagtctggtgtgcccttcttgtctatttctggttcagacttcatggaaatgtttgttggtgttggaccatccagggtgcggaacttatttcaagaagctcggcagtgttcacctagtattgtattcattgatgaaattgatgcaatcggccgtgctagaggccgtggaggtttttctggtggacatgatgagcgtgaaagcacattgaaccagttacttgtagagatggatgggtttggaactacatctggtgttgttgttcttgctggtaccaataggcctgacatcctggataaggctttgctaagaccaggaagatttgatcgtcagataagtattgataaaccagacataaagggtcgtgatcaaatatttcgcatctaccttaaaaagctgaaactggacaaggaaccatcattttattcacaaagattagctgctttgacacctggatttgctggagctgacattgccaatgtttgtaatgaggcagctttaattgctgcaagaagtgagggcacactcattacaatgcaacattttgaatctgcaattgatagggttatcggaggcttggaaaagaaaaacaaggtattttttacggtgccaattttcatttgcctctactagttgatttatcaaattttacataagaattttgcaacctggctgccaaactatccagtatccacaatgctgctaggctacgagtacataaataaacaaaattgttgcaaatactttgttgttgggctgccatactttttgttttatcaattcaggaggtagcaaattttattatatatatgttaaacaatatcggcttggtcaatgttgtcaattctatatgagaactgcttataactgttttttcttaaattgcaaataattgctatattttattcttgatgtgactgaggcgcactttgaaaccttcacatttgctttgagagcctaggttggtttgtacaattttaaaagtgatttcagtgttctctttgttacttgttacagcagaaatcattgcataattaagtgctgttgcattggatgtaaagtattatatggggcatgaacaattggacattcatgtgtagcggttatgtacttatcaatctgtccaccacaggtgattagcaagttggagcggcgaactgttgcttaccatgaatctggtcatgctgttgctggatggtttttggagcatgccgagccactactgaaggtcacaattgttcctcgtggaacagctgctttaggttttgctcagtatgtgccaaatgacaatcttttgatgactaaagagcagctttttgacatgacctgcatgacactaggtggccgagctgctgaggaggtacacaacattttctaacttatcagtagagtttattcttaagcaaatgacttaattatgtgattttttttcaggtcttgattggaaagatctcgactggtgcacagaatgatctggagaaagttactaagatgacttatgcccaggttgcagtatatggcttcagcgaaaaggttggccttctttctttccctcaaagggaggatggttttgagatgagcaaaccatatagcagccagacggcatccatcattgacactgaggtgagggaatgggtcgctaaggcctatgaaaaaactgttgagcttataaaacaacataaagatcaagttgcacaaatcgccgagctgctgcttgagaaagaggttctccaccaggatgatcttgtccaggttctaggggaacgcccattcaagacactcgagccaactaattatgatcgattcaaacaaggttttcaagatgaagacagcaataggaatgctgaattgtcaaatgcagatggagcatcatcccttggagaggctgtggcatcataacttggacagaattgtaggtgtatatcgcatgtttgctgaacagattattaatatttctgaggcatgagaagctgtataaaggtgttcatatgccaagttatggctattttgctagattgcgatttccacttcgcgcaggcgttcaatctgttgaatctgccgataactagtggttcagttcatgaattttcagaaatttacttgacgatttgatctctgttcctgtaaggctgtaacctagcactagggcatgagaagttgtataaaggtgttcgtggtttttctg</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051309.1 RefSeq:Os01g0842600]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0842600&diff=169481Os01g08426002014-05-18T09:32:47Z<p>Daweih: </p>
<hr />
<div>This gene encodes FtsH protease (OsFtsH3), homologue of AtFtsH3/10.<br />
==Annotated Information==<br />
===Function===<br />
Please input function information here.<br />
<br />
===Expression===<br />
Please input expression information here.<br />
<br />
===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
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==Labs working on this gene==<br />
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==References==<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0842600|<br />
Description = Similar to AAA-metalloprotease FtsH|<br />
Version = NM_001051309.1 GI:115440988 GeneID:4327502|<br />
Length = 5416 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0842600, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:37938310..37943725|<br />
CDS = 37938596..37939060,37939135..37939362,37939901..37940668,37940838..37941320,37942471..37942598<br>,37943127..37943195,37943276..37943480,37943593..37943655|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:37938310..37943725<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:37938310..37943725<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgagcctctcctccctctcccgcgccctcgcccggtccgcgcgctcctcccggcagcgccagggttctctgctcggggggcatggcgggctccgtgcttcgtcgccgccgctgccctgcggcgagttgggttttctgcgcagctatgtgacgtcagttatcgggaatcgggcagcggtggcctccggggcagggaagggtggggactggaggttcctgcttgctagccggcagttccggcggctattctccgacaagtcgaagaaaaaccacgggaagcattcggaggaggagaataaaggtaaaggggatgagagtgacaagtccgactcaaagaagcaatccagttcaggtgatcaatggaatttcgaggagagcattaaacaattcaaggacatgattgctcctttattcctttttggcctattattgttatctgcatcagcatcttcatcagagcaggagataagcttccaagaattcaagaataagttgttggaacctggtttagttgaccacattgttgtttcaaacaagtcaattgctaaggtttacgtcaggagttcaccttcaattgatcgaattcaggatagtgatatccatataactacaagtcatcttcctgggatagagtctcctagcagctacaagtattacttcaatattggaagtgttgactcctttgaagagaagttacaagaggcccagaaagcattggagatagatccacactattatgtcccaatcacttacactactgaagcaaaatggtttgaggaagtaatgaagtatgtcccaaccgttctaattattggattaatatatttgcttgggaaaaggatacaaaatggatttactgttgggggtggtcctgggaaaggaggccgtagcattttcagtattggaaaagttcaagtgacaaaattggataaaaactccaaaaataaggtgttctttaaggatgtagctggctgtgatgaagccaaacaagaaattatggaatttgttcatttcctgaaaaatcccaagaaatatgaagaactgggagcaaaaataccaaagggtgctcttcttgttggtcccccaggcacaggaaagactctccttgctaaagctactgcaggagagtctggtgtgcccttcttgtctatttctggttcagacttcatggaaatgtttgttggtgttggaccatccagggtgcggaacttatttcaagaagctcggcagtgttcacctagtattgtattcattgatgaaattgatgcaatcggccgtgctagaggccgtggaggtttttctggtggacatgatgagcgtgaaagcacattgaaccagttacttgtagagatggatgggtttggaactacatctggtgttgttgttcttgctggtaccaataggcctgacatcctggataaggctttgctaagaccaggaagatttgatcgtcagataagtattgataaaccagacataaagggtcgtgatcaaatatttcgcatctaccttaaaaagctgaaactggacaaggaaccatcattttattcacaaagattagctgctttgacacctggatttgctggagctgacattgccaatgtttgtaatgaggcagctttaattgctgcaagaagtgagggcacactcattacaatgcaacattttgaatctgcaattgatagggttatcggaggcttggaaaagaaaaacaaggtgattagcaagttggagcggcgaactgttgcttaccatgaatctggtcatgctgttgctggatggtttttggagcatgccgagccactactgaaggtcacaattgttcctcgtggaacagctgctttaggttttgctcagtatgtgccaaatgacaatcttttgatgactaaagagcagctttttgacatgacctgcatgacactaggtggccgagctgctgaggaggtcttgattggaaagatctcgactggtgcacagaatgatctggagaaagttactaagatgacttatgcccaggttgcagtatatggcttcagcgaaaaggttggccttctttctttccctcaaagggaggatggttttgagatgagcaaaccatatagcagccagacggcatccatcattgacactgaggtgagggaatgggtcgctaaggcctatgaaaaaactgttgagcttataaaacaacataaagatcaagttgcacaaatcgccgagctgctgcttgagaaagaggttctccaccaggatgatcttgtccaggttctaggggaacgcccattcaagacactcgagccaactaattatgatcgattcaaacaaggttttcaagatgaagacagcaataggaatgctgaattgtcaaatgcagatggagcatcatcccttggagaggctgtggcatcataa</cdnaseq>|<br />
AA = <aaseq>MSLSSLSRALARSARSSRQRQGSLLGGHGGLRASSPPLPCGELG FLRSYVTSVIGNRAAVASGAGKGGDWRFLLASRQFRRLFSDKSKKNHGKHSEEENKGK GDESDKSDSKKQSSSGDQWNFEESIKQFKDMIAPLFLFGLLLLSASASSSEQEISFQE FKNKLLEPGLVDHIVVSNKSIAKVYVRSSPSIDRIQDSDIHITTSHLPGIESPSSYKY YFNIGSVDSFEEKLQEAQKALEIDPHYYVPITYTTEAKWFEEVMKYVPTVLIIGLIYL LGKRIQNGFTVGGGPGKGGRSIFSIGKVQVTKLDKNSKNKVFFKDVAGCDEAKQEIME FVHFLKNPKKYEELGAKIPKGALLVGPPGTGKTLLAKATAGESGVPFLSISGSDFMEM FVGVGPSRVRNLFQEARQCSPSIVFIDEIDAIGRARGRGGFSGGHDERESTLNQLLVE MDGFGTTSGVVVLAGTNRPDILDKALLRPGRFDRQISIDKPDIKGRDQIFRIYLKKLK LDKEPSFYSQRLAALTPGFAGADIANVCNEAALIAARSEGTLITMQHFESAIDRVIGG LEKKNKVISKLERRTVAYHESGHAVAGWFLEHAEPLLKVTIVPRGTAALGFAQYVPND NLLMTKEQLFDMTCMTLGGRAAEEVLIGKISTGAQNDLEKVTKMTYAQVAVYGFSEKV GLLSFPQREDGFEMSKPYSSQTASIIDTEVREWVAKAYEKTVELIKQHKDQVAQIAEL LLEKEVLHQDDLVQVLGERPFKTLEPTNYDRFKQGFQDEDSNRNAELSNADGASSLGE AVAS</aaseq>|<br />
DNA = <dnaseqindica>4666..5130#4364..4591#3058..3825#2406..2888#1128..1255#531..599#246..450#71..133#gggatcaaaaccctaatacggcccgcccccgcctcgatctccgtccaatgcgctcgccgccgccgccgccatgagcctctcctccctctcccgcgccctcgcccggtccgcgcgctcctcccggcagcgccaggtgagtgcccgagccgcaccccctccccttcccctctccgattggtgtggcttcttcctgcgcgtcctcgccctaatggctcgggcggcgtgtgcggatgtcgttgccgtagggttctctgctcggggggcatggcgggctccgtgcttcgtcgccgccgctgccctgcggcgagttgggttttctgcgcagctatgtgacgtcagttatcgggaatcgggcagcggtggcctccggggcagggaagggtggggactggaggttcctgcttgctagccggcagttccggcggctattctccgacaagtcgaagaaaagtaagctttctggctggttaggtggtttttggggatatctggtgactctgttaatatttaacgttgccttttttattcagaccacgggaagcattcggaggaggagaataaaggtaaaggggatgagagtgacaagtccgactcaaagagtaagtgcttattttaacctgcattcctcgctcttgtgttggatatggtttattaaggtgttgagctttgcaaaatattttgaaagagagtagacttttacataccagtggcgacttaaccttccgagtgctgccctaaaatcatgctatatcataacttgtgatttctagttttagtatactgataatagaaaacaatgttaagaagatgatatgcagagcttttgtaagtggcaatacatgttaaacagagtggggctagttgatgttttattttattgcacaaatacccctaacagtatgtaagtatcatgtatagctgaaaatgctaatatttgagttttagtgcctcataaactgcccgctcacctttaagggagaggtactagcatgagtattgtggaatacgacaacatgagcctttagcccaaagcaagttggttgcgtacaatttgtaaagtttgatgcatccgtatatagcattatactgcaaaccacatacttaatgtggtcatttctggtgtgcagagcaatccagttcaggtgatcaatggaatttcgaggagagcattaaacaattcaaggacatgattgctcctttattcctttttggcctattattgttatctgcatcagcatcttcatcagagcaggaggtctgttcctactgaaaaaccgactgttaaacatgagcaacagtagtggcttgtgtgtttcattgtcattttacctgttcacacttgtggatagaagatagctgttatcaaaatccccatttactttactgaaggtcctcgcctttggtcagccttataaatttcagaaaagaatcattccaattctgtgtggtatataaattttgtcagtgttcattttgctgcagtccaattagagaggtgatatgaggttatatataaggttactttttttatcttgaaaaaaatatttggtggtttcgctggcctagcaaagtggtaaggaaaaaaaaattatgctttcacatgtagtccttagtttatgttttatttgtacataacacaattgatgataattaattagctgcccccctccattcctaaataattttcactatactattcatatcctaagtttgacttctcatattttatcaagaaacttgtaaatatttaaaagtatactccctccatccctagatgtaagggattctagctatgaatctggacacgtgcatgtccagatccatatatttatagccagaattccttaaatttagagacagagggagtactatattatgaaattatattacatgctaaatatgatcatactgttttcattcatcaaaacatgatatatatatatatttttaaaaaagttgtggtcaaagttgtaacagcaccggttaatggtgacaagcaaatgtaaacagagggagtatgactgaagttatggttgactgaaaagatttaaatcacatagttgtactgatggagatggtatgcttttgctcgagagcattaatgtgattatttggtgcagcatgtgaccctagctacagtacttcattactgatggcatatcatatcctatcctaagagtttgatatagggcattttagcatgagcttcgtatgttgttagtgccatgttggtatgaaatgcaatatggttggtattctacatcctattaatcatctaatcctgtataactatatcatatatccacacttcagttccctaccagtttctttttctgtgcgacacatacattcatttcctagtccattataacttgtgatacccacttaattgtaattcttttcagataagcttccaagaattcaagaataagttgttggaacctggtttagttgaccacattgttgtttcaaacaagtcaattgctaaggtttacgtcaggagttcaccttcaattgatcgaattcaggatagtgatatccatataactacaagtcatcttcctgggatagagtctcctagcagctacaagtattacttcaatattggaagtgttgactcctttgaagagaagttacaagaggcccagaaagcattggagatagatccacactattatgtcccaatcacttacactactgaagcaaaatggtttgaggaagtaatgaagtatgtcccaaccgttctaattattggattaatatatttgcttgggaaaaggatacaaaatggatttactgttgggggtggtcctgggaaaggaggccgtagcattttcagtattggaaaagttcaagtgacaaaattggataaaaactccaaaaataaggttagtttttatttgctatttccatctcttgctattttgaacaaaacatgaaattcatatccttttttgtaaatgtttgttctgtagctttattcaccattgacaaagaacatacaataacatattcatataattctactgaagtaaccttgtttcttgtgtgctacaggtgttctttaaggatgtagctggctgtgatgaagccaaacaagaaattatggaatttgttcatttcctgaaaaatcccaagaaatatgaagaactgggagcaaaaataccaaagggtgctcttcttgttggtcccccaggcacaggaaagactctccttgctaaagctactgcaggagagtctggtgtgcccttcttgtctatttctggttcagacttcatggaaatgtttgttggtgttggaccatccagggtgcggaacttatttcaagaagctcggcagtgttcacctagtattgtattcattgatgaaattgatgcaatcggccgtgctagaggccgtggaggtttttctggtggacatgatgagcgtgaaagcacattgaaccagttacttgtagagatggatgggtttggaactacatctggtgttgttgttcttgctggtaccaataggcctgacatcctggataaggctttgctaagaccaggaagatttgatcgtcagataagtattgataaaccagacataaagggtcgtgatcaaatatttcgcatctaccttaaaaagctgaaactggacaaggaaccatcattttattcacaaagattagctgctttgacacctggatttgctggagctgacattgccaatgtttgtaatgaggcagctttaattgctgcaagaagtgagggcacactcattacaatgcaacattttgaatctgcaattgatagggttatcggaggcttggaaaagaaaaacaaggtattttttacggtgccaattttcatttgcctctactagttgatttatcaaattttacataagaattttgcaacctggctgccaaactatccagtatccacaatgctgctaggctacgagtacataaataaacaaaattgttgcaaatactttgttgttgggctgccatactttttgttttatcaattcaggaggtagcaaattttattatatatatgttaaacaatatcggcttggtcaatgttgtcaattctatatgagaactgcttataactgttttttcttaaattgcaaataattgctatattttattcttgatgtgactgaggcgcactttgaaaccttcacatttgctttgagagcctaggttggtttgtacaattttaaaagtgatttcagtgttctctttgttacttgttacagcagaaatcattgcataattaagtgctgttgcattggatgtaaagtattatatggggcatgaacaattggacattcatgtgtagcggttatgtacttatcaatctgtccaccacaggtgattagcaagttggagcggcgaactgttgcttaccatgaatctggtcatgctgttgctggatggtttttggagcatgccgagccactactgaaggtcacaattgttcctcgtggaacagctgctttaggttttgctcagtatgtgccaaatgacaatcttttgatgactaaagagcagctttttgacatgacctgcatgacactaggtggccgagctgctgaggaggtacacaacattttctaacttatcagtagagtttattcttaagcaaatgacttaattatgtgattttttttcaggtcttgattggaaagatctcgactggtgcacagaatgatctggagaaagttactaagatgacttatgcccaggttgcagtatatggcttcagcgaaaaggttggccttctttctttccctcaaagggaggatggttttgagatgagcaaaccatatagcagccagacggcatccatcattgacactgaggtgagggaatgggtcgctaaggcctatgaaaaaactgttgagcttataaaacaacataaagatcaagttgcacaaatcgccgagctgctgcttgagaaagaggttctccaccaggatgatcttgtccaggttctaggggaacgcccattcaagacactcgagccaactaattatgatcgattcaaacaaggttttcaagatgaagacagcaataggaatgctgaattgtcaaatgcagatggagcatcatcccttggagaggctgtggcatcataacttggacagaattgtaggtgtatatcgcatgtttgctgaacagattattaatatttctgaggcatgagaagctgtataaaggtgttcatatgccaagttatggctattttgctagattgcgatttccacttcgcgcaggcgttcaatctgttgaatctgccgataactagtggttcagttcatgaattttcagaaatttacttgacgatttgatctctgttcctgtaaggctgtaacctagcactagggcatgagaagttgtataaaggtgttcgtggtttttctg</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051309.1 RefSeq:Os01g0842600]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0574500&diff=169480Os01g05745002014-05-18T09:26:34Z<p>Daweih: </p>
<hr />
<div>This gene encode FtsH protease (OsFtsH5), homologue of AtFtsH4.<br />
==Annotated Information==<br />
===Function===<br />
Please input function information here.<br />
<br />
===Expression===<br />
Please input expression information here.<br />
<br />
===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Please input related labs here.<br />
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==References==<br />
Please input cited references here.<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os01g0574500|<br />
Description = Peptidase M41, FtsH domain containing protein|<br />
Version = NM_001049920.1 GI:115437803 GeneID:4326311|<br />
Length = 5942 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0574500, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:23739143..23745084|<br />
CDS = 23739386..23739412,23740004..23740091,23740404..23740664,23741428..23741685,23741771..23741895<br>,23743110..23743586,23743672..23744583|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:23739143..23745084<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:23739143..23745084<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggcgtggcggcgcgtcctttcgcaggtcgcaaggaaccgatccgcatatgcaatctgcaatgaaataatatatgccaacccatcgaggattcttagaggtgacaccatagcaggtggaacacttagaaacttacatgagaggtatcagtcaagctatgttggtagctttgcacgcaggatgcgacagatggactcaccaagtgaagcctctctgctgaaagaaatatataggagtgatccagaacgtgtcatacagatatttgaaagccaaccttcattgcattccaatccttcagcacttgctgagtatgtaaaagctcttgtcagggttgataggttggaggatagcaccttgctgaaaacattgcagagaggaattgctgcttcagcaagggaggaagaaaaccttggtagtgtttcagaaaatcttggtagtgtttcagcatttagaagtgctggtcaagtgacaaaagatggaattcttggcactgcaaatgcacccattcacatggttacagcagaaacaggtcaatttaaggaacagctttggcgtacctttcgaagtatagcgctaacattcctcctgatttctggcattggagctcttattgaggatagaggaataagcaaaggccttggtctaaatgaagaggttcaaccaagcatggaatctaatacgaaattcagtgatgtaaagggtgtcgatgaagccaaagctgaacttgaggaaatagttcactacctccgagatcccaagcgttttacacgtcttggtggaaaactgccaaaaggtgtcttgctggttggtcctcctggcactggaaagaccatgttagcaagggctattgctggggaagctggtgttcctttcttctcatgcagtggtagtgaatttgaggagatgtttgtaggcgtaggagctagaagagtcagggacctttttgctgcagctaaaaaacgttctccctgtatcatattcatggatgaaattgatgcgatcggtgggagtagaaatccaaaggatcaacagtatatgaagatgacgttgaaccagttgcttgtcgagctggatggctttaagcagaatgaggggataattgttattgctgcaaccaactttcctgagtccctagataaagcactagtgagacctgggcgctttgaccggcatattgttgttccaaatccagatgttgaaggccggcggcaaatcctagagtcccatatgtcaaaggtcttgaagagtgatgatgtggatttgatgatcattgccaggggaacacctggattctcaggtgcagaccttgctaacttggttaatgtggctgctcttaaagctgccatggatggggcaaaagccgttacaatgaatgatcttgagtatgccaaggaccggatcatgatgggcagtgagcgcaagtcagcagttatttctgatgaatcccggaaattgacggcgtaccatgagggagggcacgcccttgttgccatccacactgaaggtgctcaccctgttcacaaagctaccatagttcccaggggaatggcccttggaatggtggcccaactcccggataaagatgagacaagcgtgtcacggaaacagatgctggcaaggctggatgtgtgcatgggagggcgggtggcggaggagcttatttttggggatagcgaggtgacatctggcgcttcatctgattttcagcaagcaactgcagtggcaagagcaatggtaaccaaatatggtatgagcaagcagcttggctttgtttcttacaattatgaggatgatggaaagagcatgagcaccgaaacgagactcctgattgagaaggaggtcaagtgtttcgtggagaatgcttacaacaatgcaaagaatattctcatcaaacacaacaaggagctccatgctctagccaatgcgctgcttgagcacgagacgctcaccggtgcccaaataaagaacatattggcccaagtaaacaacaagcagcagcaggagcatgctattgaagctccacagaagacaccagctgtaccatcatctccagctgcatcagctgcagcggcagcggcagctgcagcagccgcagcacagcaagctgcagcgaaagcgaagggtgaaatcgccggtatcggatcttag</cdnaseq>|<br />
AA = <aaseq>MAWRRVLSQVARNRSAYAICNEIIYANPSRILRGDTIAGGTLRN LHERYQSSYVGSFARRMRQMDSPSEASLLKEIYRSDPERVIQIFESQPSLHSNPSALA EYVKALVRVDRLEDSTLLKTLQRGIAASAREEENLGSVSENLGSVSAFRSAGQVTKDG ILGTANAPIHMVTAETGQFKEQLWRTFRSIALTFLLISGIGALIEDRGISKGLGLNEE VQPSMESNTKFSDVKGVDEAKAELEEIVHYLRDPKRFTRLGGKLPKGVLLVGPPGTGK TMLARAIAGEAGVPFFSCSGSEFEEMFVGVGARRVRDLFAAAKKRSPCIIFMDEIDAI GGSRNPKDQQYMKMTLNQLLVELDGFKQNEGIIVIAATNFPESLDKALVRPGRFDRHI VVPNPDVEGRRQILESHMSKVLKSDDVDLMIIARGTPGFSGADLANLVNVAALKAAMD GAKAVTMNDLEYAKDRIMMGSERKSAVISDESRKLTAYHEGGHALVAIHTEGAHPVHK ATIVPRGMALGMVAQLPDKDETSVSRKQMLARLDVCMGGRVAEELIFGDSEVTSGASS DFQQATAVARAMVTKYGMSKQLGFVSYNYEDDGKSMSTETRLLIEKEVKCFVENAYNN AKNILIKHNKELHALANALLEHETLTGAQIKNILAQVNNKQQQEHAIEAPQKTPAVPS SPAASAAAAAAAAAAAAQQAAAKAKGEIAGIGS</aaseq>|<br />
DNA = <dnaseqindica>244..270#862..949#1262..1522#2286..2543#2629..2753#3968..4444#4530..5441#gtgtccccctgcgccgcgtgaccgtcaactgacgtggcccatccctcctctcttccccccacctcgccgccggcgaattcctcgcctccgccgcgaccgagcccccgccgctcttggggcttcgctcgcttctccagcgcaaccctcccccccctcccccgccgccgccctcacacagggttctcttgcgggttggggatctcatcgcggcggcggcggcggactaataagggattccggccgatggcgtggcggcgcgtcctttcgcaggtgcggtttccttccggcctttctctctctctctctctctctctctctctctcttctctctctctctctctctctctctctctcgctcgtgggttgtgggttgtggtcggggaggagggctcgatcttgcgcggtgggcgcggggacgcagggggctgaggggtgggggttggtttcgtttcctgttaagataagagtagtaatcttgggagatgttcgtgtgggtgcccgtattggatggaagggtcgatgctccgcgccgcgattgagcttctgtttcttgacctggattagcgtgatcctgttcgtgctggatgctttgtggttttagactaatccgttgaaaacagcacggcaaccttgttttcatgtcatatggcttgtcttttatgtttcgatatttatcggattggcatgacaatctctatgagaattttgttggagatagctaaaattgagtccaggacccgcttagcttgtaggcatgcgttaattggagcttgtaatcatcaaaaggggactgcaattgaataaggattggtgctacaatggtttactgaacgtaaggttatgctgtttttctcatcacaggtcgcaaggaaccgatccgcatatgcaatctgcaatgaaataatatatgccaacccatcgaggattcttagaggtgacaccatagcaggtatgcagtctttgtattatatatgccaactcaatcattttagtaatgcaatagtgggttctaaaattgcaagtttcaaaatttatgatagatatgaggttcagtcttggatatctggcatatagtcctccccatattaaacatataccttcattggtgtgttcctcctttttccttttctggagatgtctttaaagctgtattatcatattactttcttatgagcagtgctattgtttccatttcctcctgttgtgatgaatttgtttgcactttcatattgagttatggtttttggggtgcttaatgcaggtggaacacttagaaacttacatgagaggtatcagtcaagctatgttggtagctttgcacgcaggatgcgacagatggactcaccaagtgaagcctctctgctgaaagaaatatataggagtgatccagaacgtgtcatacagatatttgaaagccaaccttcattgcattccaatccttcagcacttgctgagtatgtaaaagctcttgtcagggttgataggttggaggatagcaccttgctgaaaacattgcagagaggtactaataccgaccaacactttatctgtttcaggactattctgctgatttttgtgtgggttatgctactagtgtttattttccttatttgaagactggagaatgcttcattagcttgccaaacacttccaatgtagatctgttgtcatgttagtcttacatttggtgtcatgattgttcagtttgtcattaagccacacaaggaaattacaatataacagcagtaaacaattcttcatatgctagcacatatatgttaccttcaaaacttcatctcctactgtagttcagcttactgtaatttgtaattcttcaagcctgtggactgtggttaccgtagttcaagtcttacaaggaaattactacacatttcagcagttaacaaaatcatggataatcatcttaatatggcatagaaattttgccatgaaaaaaaatcatcctctattagtctattgtagttccaattatattttttgtagttcttcatggcctgttggttatcatgatgtttggttattgatttttacaactgtgggatgggtcataatcttgacccacctattcctatcttacaagggtagtcctatttgattttgtcttctcaagataggttgagagaaactaatttgttgtatagtactgtccttgtctgttgttgactaaactacaactttgaagccttcttacagttccttcaattaataatggtgattctttgattaacttggtctaaaattacatccttgcttcatccttacaggaattgctgcttcagcaagggaggaagaaaaccttggtagtgtttcagaaaatcttggtagtgtttcagcatttagaagtgctggtcaagtgacaaaagatggaattcttggcactgcaaatgcacccattcacatggttacagcagaaacaggtcaatttaaggaacagctttggcgtacctttcgaagtatagcgctaacattcctcctgatttctggcattggagctcttattgaggatagaggaataagcaaaggtttgaattctgaaagatctgaatggctaattccttgtttactgaacttattcctgtgtagttctgattgataaaatatgtgtaggccttggtctaaatgaagaggttcaaccaagcatggaatctaatacgaaattcagtgatgtaaagggtgtcgatgaagccaaagctgaacttgaggaaatagttcactacctccgagatcccaaggtaaatgcttttgagcccctagtcttgaaaactggtaacattgtctagctgtttggatgcagagttatctgaacatgtttcttacaggtttgttccccccatctgataatggttttatattgcagtttttgttaatagacattaatatttaagaggttcaagcccagttgtccaagtgcaatggattaattttgacatactaacgatgtaaagtttaaaactgttctgcaatagatttatctctgactttcttcattgtactgaatcacgaggtattgataagctaactcagtttatctatcaatatcctgattgattcaaaacctttgtttgatttcgttgacagtttccagcttgttagttctattacatgcacgctgttactttatttggtaaatgcattatagatataccttttcaatttcattgttaattctaggttgtttagctcacttattataatttagataggggtaaatttcacattggaccatatatttgaccaaggtttcacaatggacaaggactaaaccagcattttaccacatatcttagcatttttggtagtttggactttggactagggttgagcacatcaagggaatatgtgagaaaaaaagctaatccaaacaatcattttagtgtttcataatagtaagataaatatatttagagaattccgtcaaatggtattttagcaaatcgaaaaacaagttttttcaacagatcacaggaaacagtgatgtgaacatgttctaaaaagggcacaaattaatctacacagactcacagaggagcaaatgaataaaaacgaaatataaatagaacttattgattagataccactaaaaagaatgaacaaaacatacaatgtgttacctcctattttggtattcatccatgcttaaccacataaagttctcacatattctcttggtatgcttacccctagtccaaaccacaaactaatgaaataatatacatggtgttatgtgaaaacattgattaatccctggtacattatgaaactttggtcaaaatatgtggccaatgtgaaatttactatttagataatgtgtggaattgatgtccggttatgtcacatatatctgtttatgcatcgttaagcccataaatggtgtgaggatttacccctcattttcatcacatgcatatgttatacggcaaatgacaagctgcttccttgttttcagcgttttacacgtcttggtggaaaactgccaaaaggtgtcttgctggttggtcctcctggcactggaaagaccatgttagcaagggctattgctggggaagctggtgttcctttcttctcatgcagtggtagtgaatttgaggagatgtttgtaggcgtaggagctagaagagtcagggacctttttgctgcagctaaaaaacgttctccctgtatcatattcatggatgaaattgatgcgatcggtgggagtagaaatccaaaggatcaacagtatatgaagatgacgttgaaccagttgcttgtcgagctggatggctttaagcagaatgaggggataattgttattgctgcaaccaactttcctgagtccctagataaagcactagtgagacctgggcgctttgaccggcatattgttgttccaaatccagatgttgaaggccggcggcaaatcctagagtcccatatgtcaaaggtaattcttgagaacttttggtatcatgtttgtccagactaagatggaatgtcttatatttatccttatacacatatggttacaggtcttgaagagtgatgatgtggatttgatgatcattgccaggggaacacctggattctcaggtgcagaccttgctaacttggttaatgtggctgctcttaaagctgccatggatggggcaaaagccgttacaatgaatgatcttgagtatgccaaggaccggatcatgatgggcagtgagcgcaagtcagcagttatttctgatgaatcccggaaattgacggcgtaccatgagggagggcacgcccttgttgccatccacactgaaggtgctcaccctgttcacaaagctaccatagttcccaggggaatggcccttggaatggtggcccaactcccggataaagatgagacaagcgtgtcacggaaacagatgctggcaaggctggatgtgtgcatgggagggcgggtggcggaggagcttatttttggggatagcgaggtgacatctggcgcttcatctgattttcagcaagcaactgcagtggcaagagcaatggtaaccaaatatggtatgagcaagcagcttggctttgtttcttacaattatgaggatgatggaaagagcatgagcaccgaaacgagactcctgattgagaaggaggtcaagtgtttcgtggagaatgcttacaacaatgcaaagaatattctcatcaaacacaacaaggagctccatgctctagccaatgcgctgcttgagcacgagacgctcaccggtgcccaaataaagaacatattggcccaagtaaacaacaagcagcagcaggagcatgctattgaagctccacagaagacaccagctgtaccatcatctccagctgcatcagctgcagcggcagcggcagctgcagcagccgcagcacagcaagctgcagcgaaagcgaagggtgaaatcgccggtatcggatcttagcagtcaccaaatgatgttggatcgattgacagctcttgaggatcacgaagagtttgctaatgctgctgcagtcatcaatagaacatattgattgtgtgtaagctgttatttcgtttcatgttcctcaagtgtgtggtaagaaaacataagagaaatcataacattgtagaaggcacatttagactctgcttgatatgtgaatttttgtatcacatggccacacacacggaaatactgtttacatcctaataattttcagctaccttagacgttggatggttatacctaacagagaacctgttgtatttgtctagaatggtagctccagagtccatttctttagccctgtgatgctcccttgttgtactgtatgttctgcaagttgacaaaagaagtttttctgtatctttgctgaattgccgtgtaatgacaaacagtaatgttggggccagatactcgccaattgcaggtattctgtcatcaatcagttttttattcctg</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049920.1 RefSeq:Os01g0574500]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800/QualityDetail&diff=75320Os01g0883800/QualityDetail2012-07-17T16:17:31Z<p>Daweih: 1</p>
<hr />
<div><table border="1"><tr><th>Contributor</th><th>Edit Quality</th></tr><tr><td>[[Special:ScienceReward|Hao Wu]]<br />
</td><td> 0.49 | 0.98 | 0.88<br />
</td></tr><tr><td>[[Special:ScienceReward|Dawei Huang]]<br />
</td><td> 0.14 | -0.54 | -0.15 | 0.01 | -0.15 | 0.79 | -0.19 | 0.17 | 0.16 | -0.12 | 1 | -0.88 | 0.4<br />
</td></tr><tr><td>[[Special:ScienceReward|Lina Ma]]<br />
</td><td> -1 | -1 | 0.04 | 0.13 | -0.08 | -0.02 | -0.09 | 0.05 | -0.01 | 0.02 | -0.01 | 0.01 | 0.29 | -0.1 | 0.18 | 0.54 | -0.76<br />
</td></tr><tr><td>[[Special:ScienceReward|Ang Li]]<br />
</td><td> 0.98 | 1<br />
</td></tr><tr><td>[[Special:ScienceReward|Rice2012]]<br />
</td><td> -1 | 0.37 | -1<br />
</td></tr><tr><td>[[Special:ScienceReward|Chao Xu]]<br />
</td><td> -0.5 | -1 | -1 | -0.43 | 0.96 | 1<br />
</td></tr><tr><td>[[Special:ScienceReward|sangjian]]<br />
</td><td> 0.55 | -0.86 | 0.7 | -0.17 | -1 | -1 | -1 | 1<br />
</td></tr></table><br />
Each contributor may involve multiple revisions, which are separated by '|'.</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800/QuantityDetail&diff=75319Os01g0883800/QuantityDetail2012-07-17T16:17:26Z<p>Daweih: 1</p>
<hr />
<div><table border="1"><tr><th>Contributor</th><th>Edit Quantity</th></tr><tr><td>[[Special:ScienceReward|Hao Wu]]<br />
</td><td> 6523 | 638 | 2668<br />
</td></tr><tr><td>[[Special:ScienceReward|Dawei Huang]]<br />
</td><td> 4043 | 1071 | 585 | 180 | 240 | 3675 | 1973 | 5470 | 17 | 68 | 1950 | 306 | 646<br />
</td></tr><tr><td>[[Special:ScienceReward|Lina Ma]]<br />
</td><td> 3984 | 17 | 2428 | 5114 | 2278 | 2800 | 60 | 1360 | 5777 | 4092 | 68 | 7604 | 6969 | 376 | 6991 | 6555 | 109<br />
</td></tr><tr><td>[[Special:ScienceReward|Ang Li]]<br />
</td><td> 529 | 192<br />
</td></tr><tr><td>[[Special:ScienceReward|Rice2012]]<br />
</td><td> 25 | 1939 | 75<br />
</td></tr><tr><td>[[Special:ScienceReward|Chao Xu]]<br />
</td><td> 552 | 72 | 2 | 524 | 174 | 1<br />
</td></tr><tr><td>[[Special:ScienceReward|sangjian]]<br />
</td><td> 90 | 5992 | 6388 | 52 | 24 | 52 | 25 | 1<br />
</td></tr></table><br />
Each contributor may involve multiple revisions, which are separated by '|'.</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800&diff=73861Os01g08838002012-07-15T13:05:27Z<p>Daweih: </p>
<hr />
<div>The rice ''semidwarf-1 (sd1)'' is well known as the "green revolution gene" and controls the plant height of rice.<br />
<br />
== Annotated Information ==<br />
=== Function ===<br />
This gene is originally derived from the chinese cultivar Dee-Geo-Woo-Gen(DGWG). It encodes an oxidase enzyme involved in the biosynthesis of gibberellin, which is a plant growth hormone. The rice genome carries at least two GA20ox genes (GA20ox-1 and GA20ox-2).SD1 corresponds to GA20ox-2. Mutation of SD1 will cause a semi-dwarf phenotype of rice without seed yield being affected <ref name="ref1" />. It is not surprising that a rice semidwarfing gene encodes GA20-ox since successful production of semidwarf plants using antisense or overexpressed GA20-ox genes has been reported in Arabidopsis, Solanum dulcamara, potato, and lettuce <ref name="ref3" />.<br />
<br />
[[File:Example.jpg]] ''Semidwarf VS. normal-type rice plants at ripening (from reference <ref name="ref3" />).''<br />
<br />
It is a key enzyme in the biosynthesis of gibberellin that catalyses the three steps GA53->GA44->GA19->GA20. Impaired GA 20-oxidase activity will cause elevated content of GA53, and reduced amount of G20 <ref name="ref1" />.<ref name="ref2" />. However, there is slight difference between different strains. The extent of GA1 is lower in Doongara (semi-dwarf rice strain) when compared with Kyeema (tall), while there is no significant difference between Calrose76 (semi-dwarf rice strain) and Calrose (tall). Calrose76 has lower contents of GA44 and of GA19 than Calrose, while there is no significant difference between Doongara (semi-dwarf rice strain) and Kyeema (tall) <ref name="ref2" />.<br />
<br />
GO asignment(s): GO:0005506, GO:0016216, GO:0017000<br />
<br />
=== Mutation ===<br />
‘dee-geo-woo-gen’ (semi-dwarf rice strain): A 383-base-pair deletion from the genome (A 280-bp deletion within the coding region), which induces a frameshift that creates a stop codon in SD1, may be related with the semi-dwarf phenotype <ref name="ref1" /><ref name="ref2" />.<br />
<br />
Calrose76 (semi-dwarf rice strain): The DNA sequence of Calrose76 is identical to Calrose (tall) except for a C to T transition at position 798 that resulted in a change of the predicted amino acid leucine (Leu-266) in Calrose to phenylalanine in Calrose76 <ref name="ref2" />.<br />
<br />
=== Expression ===<br />
This gene is strongly expressed in the leaf blade, stem and unopened flower, whereas GA20ox-1 is predominantly expressed in the unopened flower <ref name="ref1" />. <br />
<br />
Among the DGWG-type sd-1 mutants, IR24 and Habataki have little transcript of this gene, while Milyang 23 expresses a normal or greater amount of truncated transcript. No significant difference is observed between Calrose and its single-nucleotide-substitution mutant Calrose 76 <ref name="ref3" />.<br />
<br />
Analysis of the tissue- and stage-specificity of transcription of sd1 in Nipponbare revealed that this<br />
gene was expressed within 48 hr after sowing, as well as in 10-day-old plants, 30-day-old leaves, and flowering panicles; no transcription is detected in 24-hr-old seedlings or in 14-day-old roots. Transcript accumulates<br />
predominantly in adult leaves <ref name="ref3" />.<br />
<br />
Primers for gene amplication: forward primer, 5'-CAACTCACTCCCGCTCAACACAGC-3', and reverse primer, 5'-TTTGAAATGCAATGTCGTCCACC-3' were used to amplify exon 1; forward primer, 5'-GCGCCAATGGGGTAATTAAAACG-3' and reverse primer, 5'-GGCATTCCATTGTTTGTGATTGG-3' were used for exon 2; forward primer, 5'-GTTTGTCCTTGTCGCGTTGCTCAG-3' and reverse primer, 5'-TCTGTTCGTTCCGTTTCGTTCCG-3' were used for exon 3 <ref name="ref2" />.<br />
<br />
Primers for RT-PCR: forward primer 5'-CAACTCACTCCCGCTCAACACAGC- 3' located 22-bp upstream from the translation start, and a reverse primer 5'-GTTCGTTCCGTTTCGGTTCCG- 3' eight-bp downstream from the TGA stop codon <ref name="ref2" />.<br />
<br />
Primers for RT-PCR: forward primer: 5'-AGCTGGACATGCCCGTGGTC-3' and reverse primer : 5'-TTGAGCTGCTGTCCGCGAAG-3' <ref name="ref3" />.<br />
<br />
=== Evolution ===<br />
This gene is conserved in ''Arabidopsis'' (47% identity) and pea(50% identity). GA20ox-2 shows 47.8% identity to<br />
GA20ox-1 in rice. There are at least three GA20-ox genes in Arabidopsis <ref name="ref1" />.<ref name="ref2" />.<br />
<br />
=== Knowledge Extension ===<br />
The wheat green-revolution gene Rht (for ‘reduced height’)<ref name="ref4" /> is a gain-of-function allele caused by a mutation in a transcription factor that is associated with the gibberellin signalling pathway. As wheat has a hexaploid genome, it does not contain recessive alleles such as sd1 in rice that might otherwise be used to produce a semi-dwarf strain of wheat. Although the genetic and biochemical functions of the rice SD1 and wheat RHT proteins are completely different (that is, recessive versus dominant, loss-of-function versus gain-offunction events, enzyme versus transcription factor, respectively), the products of both genes are linked with gibberellin malfunction <ref name="ref1" />.<br />
<br />
== Labs working on this gene ==<br />
Bioscience Center, and Graduate School of Bioagricultural Science, Nagoya University, Nagoya 464-8601, Japan<br />
Honda R&D, Wako Research Center, Wako 351-0193, Japan<br />
<br />
International Rice Research Institute, Manila, DAPO Box 7777, Philippines<br />
<br />
BioResources Center, and Plant Molecular Biology Laboratory, Riken, Tsukuba 305-0074, Japan<br />
<br />
Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra ACT 2601, Australia<br />
<br />
Plant Genome Center, 1-25-2 Kannondai, Tsukuba, Ibaraki 305-0856, Japan<br />
<br />
== References ==<br />
<references><br />
<ref name="ref1">Sasaki A, Ashikari M, Ueguchi-Tanaka M, Itoh H, Nishimura A, et al. (2002) Green revolution: a mutant gibberellin-synthesis gene in rice. Nature 416: 701-702.</ref><br />
<ref name="ref2">Spielmeyer W, Ellis MH, Chandler PM (2002) Semidwarf (sd-1), "green revolution" rice, contains a defective gibberellin 20-oxidase gene. Proc Natl Acad Sci U S A 99: 9043-9048.</ref><br />
<ref name="ref3">Monna L, Kitazawa N, Yoshino R, Suzuki J, Masuda H, et al. (2002) Positional cloning of rice semidwarfing gene, sd-1: rice "green revolution gene" encodes a mutant enzyme involved in gibberellin synthesis. DNA Res 9: 11-17.</ref><br />
<ref name="ref4">Peng J, Richards DE, Hartley NM, Murphy GP, Devos KM, et al. (1999) 'Green revolution' genes encode mutant gibberellin response modulators. Nature 400: 256-261..</ref><br />
</references><br />
<br />
<br />
== Structured Information ==<br />
{{JaponicaGene|<br />
GeneName = Os01g0883800|<br />
Description = Gibberellin 20 oxidase 2 (EC 1.14.11.-) (Gibberellin C-20 oxidase 2) (GA 20-oxidase 2) (Os20ox2) (Semidwarf-1 protein)|<br />
Version = NM_001051549.1 GI:115441468 GeneID:4325003|<br />
Length = 2743 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0883800, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:40138313..40141055|<br />
CDS = 40138313..40138869,40138972..40139293,40140765..40141055|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:40138313..40141055<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:40138313..40141055<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggtggccgagcaccccacgccaccacagccgcaccaaccaccgcccatggactccaccgccggctctggcattgccgccccggcggcggcggcggtgtgcgacctgaggatggagcccaagatcccggagccattcgtgtggccgaacggcgacgcgaggccggcgtcggcggcggagctggacatgcccgtggtcgacgtgggcgtgctccgcgacggcgacgccgaggggctgcgccgcgccgcggcgcaggtggccgccgcgtgcgccacgcacgggttcttccaggtgtccgagcacggcgtcgacgccgctctggcgcgcgccgcgctcgacggcgccagcgacttcttccgcctcccgctcgccgagaagcgccgcgcgcgccgcgtcccgggcaccgtgtccggctacaccagcgcccacgccgaccgcttcgcctccaagctcccatggaaggagaccctctccttcggcttccacgaccgcgccgccgcccccgtcgtcgccgactacttctccagcaccctcggccccgacttcgcgccaatggggagggtgtaccagaagtactgcgaggagatgaaggagctgtcgctgacgatcatggaactcctggagctgagcctgggcgtggagcgaggctactacagggagttcttcgcggacagcagctcaatcatgcggtgcaactactacccgccatgcccggagccggagcggacgctcggcacgggcccgcactgcgaccccaccgccctcaccatcctcctccaggacgacgtcggcggcctcgaggtcctcgtcgacggcgaatggcgccccgtcagccccgtccccggcgccatggtcatcaacatcggcgacaccttcatggcgctgtcgaacgggaggtataagagctgcctgcacagggcggtggtgaaccagcggcgggagcggcggtcgctggcgttcttcctgtgcccgcgggaggacagggtggtgcggccgccgccgagcgccgccacgccgcagcactacccggacttcacctgggccgacctcatgcgcttcacgcagcgccactaccgcgccgacacccgcacgctcgacgccttcacgcgctggctcgcgccgccggccgccgacgccgccgcgacggcgcaggtcgaggcggccagctga</cdnaseq>|<br />
AA = <aaseq>MVAEHPTPPQPHQPPPMDSTAGSGIAAPAAAAVCDLRMEPKIPE PFVWPNGDARPASAAELDMPVVDVGVLRDGDAEGLRRAAAQVAAACATHGFFQVSEHG VDAALARAALDGASDFFRLPLAEKRRARRVPGTVSGYTSAHADRFASKLPWKETLSFG FHDRAAAPVVADYFSSTLGPDFAPMGRVYQKYCEEMKELSLTIMELLELSLGVERGYY REFFADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDVGGLEVLVDGEWRP VSPVPGAMVINIGDTFMALSNGRYKSCLHRAVVNQRRERRSLAFFLCPREDRVVRPPP SAATPQHYPDFTWADLMRFTQRHYRADTRTLDAFTRWLAPPAADAAATAQVEAAS</aaseq>|<br />
DNA = <dnaseqindica>1..557#660..981#2453..2743#atggtggccgagcaccccacgccaccacagccgcaccaaccaccgcccatggactccaccgccggctctggcattgccgccccggcggcggcggcggtgtgcgacctgaggatggagcccaagatcccggagccattcgtgtggccgaacggcgacgcgaggccggcgtcggcggcggagctggacatgcccgtggtcgacgtgggcgtgctccgcgacggcgacgccgaggggctgcgccgcgccgcggcgcaggtggccgccgcgtgcgccacgcacgggttcttccaggtgtccgagcacggcgtcgacgccgctctggcgcgcgccgcgctcgacggcgccagcgacttcttccgcctcccgctcgccgagaagcgccgcgcgcgccgcgtcccgggcaccgtgtccggctacaccagcgcccacgccgaccgcttcgcctccaagctcccatggaaggagaccctctccttcggcttccacgaccgcgccgccgcccccgtcgtcgccgactacttctccagcaccctcggccccgacttcgcgccaatggggtaattaaaacgatggtggacgacattgcatttcaaattcaaaacaaattcaaaacacaccgaccgagattatgctgaattcaaacgcgtttgtgcgcgcaggagggtgtaccagaagtactgcgaggagatgaaggagctgtcgctgacgatcatggaactcctggagctgagcctgggcgtggagcgaggctactacagggagttcttcgcggacagcagctcaatcatgcggtgcaactactacccgccatgcccggagccggagcggacgctcggcacgggcccgcactgcgaccccaccgccctcaccatcctcctccaggacgacgtcggcggcctcgaggtcctcgtcgacggcgaatggcgccccgtcagccccgtccccggcgccatggtcatcaacatcggcgacaccttcatggtaaaccatctcctattctcctctcctctgttctcctctgcttcgaagcaacagaacaagtaattcaagcttttttttctctctcgcgcgaaattgacgagaaaaataagatcgtggtaggggcggggctttcagctgaaagcgggaagaaaccgacctgacgtgatttctctgttccaatcacaaacaatggaatgccccactcctccatgtgttatgatttatctcacatcttatagttaataggagtaagtaacaagctacttttttcatattatagttcgtttgattttttttttttaaagtttttttagttttatccaaatttattgaaaaacttagcaacgtttataataccaaattagtctcatttagtttaatattgtatatattttgataatatatttatgttatattaaaaatattactatatttttctataaacattattaaaagccatttataatataaaatggaaggagtaattaatatggatctcccccgacatgagaatattttccgatggtgtgacgacgccatgtaagcttcggtgggcctggacggccagaggtgccaacagccacgtccaacaacccctgggtccccccctaacactccaaacagtagtgagtagtgtctcgtcgcgttttagtatttgatgacaaacaaagtgtgagttgagttagccaccaccaacttgcacacgagcacatacatttgtgtccattctcgccagtcacttccatctctagtcctaactcctatctagcgatgtaagcggataatttcatcatccgtatataaacctgtttgttatagttaatttcctatataatactataacagtatacattttaaaagaaaacaaaattaggataaacaggccctgctcctatccatccatggcacttggaaggaccagactcggtcatgccatgccaagccaagatatgggttatggaagagtagagaagaggagagatgagagataagcatgcgttctcctcctcgttggatgtgtattttggagggatttgtgtagtagtagcagcggcgccgcggggacggatgcggatggtggcgctttcggtggcgttttcccgggggggttttggtttggcgcttgggggggatggcatggcgcggcgtgcggctgcacgccacacacacgcgcgcgcacgcacgtacgtcgtcgtcgccgcgggcggacggtagcttagggtggtgtgttccgcgcgcgggcgcggattgttccatgccgatcgatttggcgccaccctcgccgcggctcttgtcgcgtcgtgcgcctctctcgcgcggtttgtccttgtcgcgttgctcagccggcgacgggggcacggacattggcgatgtagccctgcacgtgtcggcctctccgttgatgaatgatgatgtatgtatgtatttttttttgtctgaaggaatttgtggggaattgttgtgtgtgcaggcgctgtcgaacgggaggtataagagctgcctgcacagggcggtggtgaaccagcggcgggagcggcggtcgctggcgttcttcctgtgcccgcgggaggacagggtggtgcggccgccgccgagcgccgccacgccgcagcactacccggacttcacctgggccgacctcatgcgcttcacgcagcgccactaccgcgccgacacccgcacgctcgacgccttcacgcgctggctcgcgccgccggccgccgacgccgccgcgacggcgcaggtcgaggcggccagctga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051549.1 RefSeq:Os01g0883800]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800/TimeDetail&diff=73857Os01g0883800/TimeDetail2012-07-15T13:03:51Z<p>Daweih: 1</p>
<hr />
<div><table border="1"><tr><th>Contributor</th><th>Edit Time</th></tr><tr><td>[[Special:ScienceReward|Hao Wu]]<br />
</td><td> 2012-07-12 21:25:12 | 2012-07-15 19:39:58 | 2012-07-15 20:25:12<br />
</td></tr><tr><td>[[Special:ScienceReward|Dawei Huang]]<br />
</td><td> 2012-07-06 20:02:20 | 2012-07-06 20:12:16 | 2012-07-06 20:16:00 | 2012-07-06 20:19:26 | 2012-07-06 20:24:50 | 2012-07-06 20:41:03 | 2012-07-13 06:03:25 | 2012-07-13 06:52:26 | 2012-07-13 06:58:33 | 2012-07-13 07:01:13 | 2012-07-15 04:58:39 | 2012-07-15 20:57:35<br />
</td></tr><tr><td>[[Special:ScienceReward|Lina Ma]]<br />
</td><td> 2012-07-12 18:52:58 | 2012-07-12 18:54:16 | 2012-07-12 18:56:29 | 2012-07-12 19:16:57 | 2012-07-12 19:24:50 | 2012-07-12 19:28:53 | 2012-07-12 19:36:54 | 2012-07-12 20:07:34 | 2012-07-12 20:36:30 | 2012-07-12 20:54:41 | 2012-07-12 23:36:21 | 2012-07-13 00:40:38 | 2012-07-13 01:02:33 | 2012-07-13 17:39:55 | 2012-07-14 01:09:09 | 2012-07-14 01:25:38 | 2012-07-14 01:27:34<br />
</td></tr><tr><td>[[Special:ScienceReward|Rice2012]]<br />
</td><td> 2012-07-08 23:02:25 | 2012-07-09 02:52:45 | 2012-07-15 03:46:31<br />
</td></tr><tr><td>[[Special:ScienceReward|Chao Xu]]<br />
</td><td> 2012-07-12 22:08:24 | 2012-07-12 22:10:12 | 2012-07-12 22:17:49 | 2012-07-12 22:42:30<br />
</td></tr><tr><td>[[Special:ScienceReward|sangjian]]<br />
</td><td> 2012-07-06 20:09:27 | 2012-07-07 19:48:44 | 2012-07-07 19:52:41 | 2012-07-07 19:59:19 | 2012-07-07 20:02:51 | 2012-07-07 20:19:50 | 2012-07-07 20:26:03<br />
</td></tr></table><br />
Each contributor may involve multiple revisions, which are separated by '|'.</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800/QualityDetail&diff=73856Os01g0883800/QualityDetail2012-07-15T13:03:45Z<p>Daweih: 1</p>
<hr />
<div><table border="1"><tr><th>Contributor</th><th>Edit Quality</th></tr><tr><td>[[Special:ScienceReward|Hao Wu]]<br />
</td><td> 0.49 | 1 | 1<br />
</td></tr><tr><td>[[Special:ScienceReward|Dawei Huang]]<br />
</td><td> 0.14 | -0.54 | -0.15 | 0.01 | -0.15 | 0.79 | -0.19 | 0.17 | 0.16 | -0.12 | 1 | 1<br />
</td></tr><tr><td>[[Special:ScienceReward|Lina Ma]]<br />
</td><td> -1 | -1 | 0.04 | 0.13 | -0.08 | -0.02 | -0.09 | 0.05 | -0.01 | 0.02 | -0.01 | 0.01 | 0.29 | -0.02 | 0.21 | 0.65 | 0.72<br />
</td></tr><tr><td>[[Special:ScienceReward|Rice2012]]<br />
</td><td> -1 | 0.37 | -0.92<br />
</td></tr><tr><td>[[Special:ScienceReward|Chao Xu]]<br />
</td><td> -0.5 | -1 | -1 | -0.43<br />
</td></tr><tr><td>[[Special:ScienceReward|sangjian]]<br />
</td><td> 0.55 | -0.86 | 0.7 | -0.17 | -1 | -1 | -1<br />
</td></tr></table><br />
Each contributor may involve multiple revisions, which are separated by '|'.</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800/QuantityDetail&diff=73855Os01g0883800/QuantityDetail2012-07-15T13:03:40Z<p>Daweih: 1</p>
<hr />
<div><table border="1"><tr><th>Contributor</th><th>Edit Quantity</th></tr><tr><td>[[Special:ScienceReward|Hao Wu]]<br />
</td><td> 6523 | 638 | 2668<br />
</td></tr><tr><td>[[Special:ScienceReward|Dawei Huang]]<br />
</td><td> 4043 | 1071 | 585 | 180 | 240 | 3675 | 1973 | 5470 | 17 | 68 | 1950 | 306<br />
</td></tr><tr><td>[[Special:ScienceReward|Lina Ma]]<br />
</td><td> 3984 | 17 | 2428 | 5114 | 2278 | 2800 | 60 | 1360 | 5777 | 4092 | 68 | 7604 | 6969 | 376 | 6991 | 6555 | 109<br />
</td></tr><tr><td>[[Special:ScienceReward|Rice2012]]<br />
</td><td> 25 | 1939 | 75<br />
</td></tr><tr><td>[[Special:ScienceReward|Chao Xu]]<br />
</td><td> 552 | 72 | 2 | 524<br />
</td></tr><tr><td>[[Special:ScienceReward|sangjian]]<br />
</td><td> 90 | 5992 | 6388 | 52 | 24 | 52 | 25<br />
</td></tr></table><br />
Each contributor may involve multiple revisions, which are separated by '|'.</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800&diff=73848Os01g08838002012-07-15T12:57:35Z<p>Daweih: </p>
<hr />
<div>The rice ''semidwarf-1 (sd1)'' is well known as the "green revolution gene" and controls the plant height of rice.<br />
<br />
== Annotated Information ==<br />
=== Function ===<br />
This gene is originally derived from the chinese cultivar Dee-Geo-Woo-Gen(DGWG). It encodes an oxidase enzyme involved in the biosynthesis of gibberellin, which is a plant growth hormone. The rice genome carries at least two GA20ox genes (GA20ox-1 and GA20ox-2).SD1 corresponds to GA20ox-2. Mutation of SD1 will cause a semi-dwarf phenotype of rice without seed yield being affected <ref name="ref1" />. It is not surprising that a rice semidwarfing gene encodes GA20-ox since successful production of semidwarf plants using antisense or overexpressed GA20-ox genes has been reported in Arabidopsis, Solanum dulcamara, potato, and lettuce <ref name="ref3" />.<br />
<br />
[[File:Example.jpg]] ''Semidwarf VS. normal-type rice plants at ripening (from reference <ref name="ref3" />).''<br />
<br />
It is a key enzyme in the biosynthesis of gibberellin that catalyses the three steps GA53->GA44->GA19->GA20. Impaired GA 20-oxidase activity will cause elevated content of GA53, and reduced amount of G20 <ref name="ref1" />.<ref name="ref2" />. However, there is slight difference between different strains. The extent of GA1 is lower in Doongara (semi-dwarf rice strain) when compared with Kyeema (tall), while there is no significant difference between Calrose76 (semi-dwarf rice strain) and Calrose (tall). Calrose76 has lower contents of GA44 and of GA19 than Calrose, while there is no significant difference between Doongara (semi-dwarf rice strain) and Kyeema (tall) <ref name="ref2" />.<br />
<br />
GO asignment(s): GO:0005506, GO:0016216, GO:0017000<br />
<br />
=== Mutation ===<br />
‘dee-geo-woo-gen’ (semi-dwarf rice strain): A 383-base-pair deletion from the genome (A 280-bp deletion within the coding region), which induces a frameshift that creates a stop codon in SD1, may be related with the semi-dwarf phenotype <ref name="ref1" /><ref name="ref2" />.<br />
<br />
Calrose76 (semi-dwarf rice strain): The DNA sequence of Calrose76 is identical to Calrose (tall) except for a C to T transition at position 798 that resulted in a change of the predicted amino acid leucine (Leu-266) in Calrose to phenylalanine in Calrose76 <ref name="ref2" />.<br />
<br />
=== Expression ===<br />
This gene is strongly expressed in the leaf blade, stem and unopened flower, whereas GA20ox-1 is predominantly expressed in the unopened flower <ref name="ref1" />. <br />
<br />
Among the DGWG-type sd-1 mutants, IR24 and Habataki have little transcript of this gene, while Milyang 23 expresses a normal or greater amount of truncated transcript. No significant difference is observed between Calrose and its single-nucleotide-substitution mutant Calrose 76 <ref name="ref3" />.<br />
<br />
Analysis of the tissue- and stage-specificity of transcription of sd1 in Nipponbare revealed that this<br />
gene was expressed within 48 hr after sowing, as well as in 10-day-old plants, 30-day-old leaves, and flowering panicles; no transcription is detected in 24-hr-old seedlings or in 14-day-old roots. Transcript accumulates<br />
predominantly in adult leaves <ref name="ref3" />.<br />
<br />
Primers for gene amplication: forward primer, 5'-CAACTCACTCCCGCTCAACACAGC-3', and reverse primer, 5'-TTTGAAATGCAATGTCGTCCACC-3' were used to amplify exon 1; forward primer, 5'-GCGCCAATGGGGTAATTAAAACG-3' and reverse primer, 5'-GGCATTCCATTGTTTGTGATTGG-3' were used for exon 2; forward primer, 5'-GTTTGTCCTTGTCGCGTTGCTCAG-3' and reverse primer, 5'-TCTGTTCGTTCCGTTTCGTTCCG-3' were used for exon 3 <ref name="ref2" />.<br />
<br />
Primers for RT-PCR: forward primer 5'-CAACTCACTCCCGCTCAACACAGC- 3' located 22-bp upstream from the translation start, and a reverse primer 5'-GTTCGTTCCGTTTCGGTTCCG- 3' eight-bp downstream from the TGA stop codon <ref name="ref2" />.<br />
<br />
Primers for RT-PCR: forward primer: 5'-AGCTGGACATGCCCGTGGTC-3' and reverse primer : 5'-TTGAGCTGCTGTCCGCGAAG-3' <ref name="ref3" />.<br />
<br />
=== Evolution ===<br />
This gene is conserved in ''Arabidopsis'' (47% identity) and pea(50% identity). GA20ox-2 shows 47.8% identity to<br />
GA20ox-1 in rice. There are at least three GA20-ox genes in Arabidopsis <ref name="ref1" />.<ref name="ref2" />.<br />
<br />
=== Knowledge Extension ===<br />
The wheat green-revolution gene Rht (for ‘reduced height’)<ref name="ref4" /> is a gain-of-function allele caused by a mutation in a transcription factor that is associated with the gibberellin signalling pathway. As wheat has a hexaploid genome, it does not contain recessive alleles such as sd1 in rice that might otherwise be used to produce a semi-dwarf strain of wheat. Although the genetic and biochemical functions of the rice SD1 and wheat RHT proteins are completely different (that is, recessive versus dominant, loss-of-function versus gain-offunction events, enzyme versus transcription factor, respectively), the products of both genes are linked with gibberellin malfunction <ref name="ref1" />.<br />
<br />
== Labs working on this gene ==<br />
Bioscience Center, and Graduate School of Bioagricultural Science, Nagoya University, Nagoya 464-8601, Japan<br />
Honda R&D, Wako Research Center, Wako 351-0193, Japan<br />
<br />
International Rice Research Institute, Manila, DAPO Box 7777, Philippines<br />
<br />
BioResources Center, and Plant Molecular Biology Laboratory, Riken, Tsukuba 305-0074, Japan<br />
<br />
Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra ACT 2601, Australia<br />
<br />
Plant Genome Center, 1-25-2 Kannondai, Tsukuba, Ibaraki 305-0856, Japan<br />
<br />
== References ==<br />
WHERE CITE<ref name="ref3" />？<br />
<br />
<references><br />
<ref name="ref1">Sasaki A, Ashikari M, Ueguchi-Tanaka M, Itoh H, Nishimura A, et al. (2002) Green revolution: a mutant gibberellin-synthesis gene in rice. Nature 416: 701-702.</ref><br />
<ref name="ref2">Spielmeyer W, Ellis MH, Chandler PM (2002) Semidwarf (sd-1), "green revolution" rice, contains a defective gibberellin 20-oxidase gene. Proc Natl Acad Sci U S A 99: 9043-9048.</ref><br />
<ref name="ref3">Monna L, Kitazawa N, Yoshino R, Suzuki J, Masuda H, et al. (2002) Positional cloning of rice semidwarfing gene, sd-1: rice "green revolution gene" encodes a mutant enzyme involved in gibberellin synthesis. DNA Res 9: 11-17.</ref><br />
<ref name="ref4">Peng J, Richards DE, Hartley NM, Murphy GP, Devos KM, et al. (1999) 'Green revolution' genes encode mutant gibberellin response modulators. Nature 400: 256-261..</ref><br />
</references><br />
<br />
<br />
== Structured Information ==<br />
{{JaponicaGene|<br />
GeneName = Os01g0883800|<br />
Description = Gibberellin 20 oxidase 2 (EC 1.14.11.-) (Gibberellin C-20 oxidase 2) (GA 20-oxidase 2) (Os20ox2) (Semidwarf-1 protein)|<br />
Version = NM_001051549.1 GI:115441468 GeneID:4325003|<br />
Length = 2743 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0883800, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:40138313..40141055|<br />
CDS = 40138313..40138869,40138972..40139293,40140765..40141055|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:40138313..40141055<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:40138313..40141055<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggtggccgagcaccccacgccaccacagccgcaccaaccaccgcccatggactccaccgccggctctggcattgccgccccggcggcggcggcggtgtgcgacctgaggatggagcccaagatcccggagccattcgtgtggccgaacggcgacgcgaggccggcgtcggcggcggagctggacatgcccgtggtcgacgtgggcgtgctccgcgacggcgacgccgaggggctgcgccgcgccgcggcgcaggtggccgccgcgtgcgccacgcacgggttcttccaggtgtccgagcacggcgtcgacgccgctctggcgcgcgccgcgctcgacggcgccagcgacttcttccgcctcccgctcgccgagaagcgccgcgcgcgccgcgtcccgggcaccgtgtccggctacaccagcgcccacgccgaccgcttcgcctccaagctcccatggaaggagaccctctccttcggcttccacgaccgcgccgccgcccccgtcgtcgccgactacttctccagcaccctcggccccgacttcgcgccaatggggagggtgtaccagaagtactgcgaggagatgaaggagctgtcgctgacgatcatggaactcctggagctgagcctgggcgtggagcgaggctactacagggagttcttcgcggacagcagctcaatcatgcggtgcaactactacccgccatgcccggagccggagcggacgctcggcacgggcccgcactgcgaccccaccgccctcaccatcctcctccaggacgacgtcggcggcctcgaggtcctcgtcgacggcgaatggcgccccgtcagccccgtccccggcgccatggtcatcaacatcggcgacaccttcatggcgctgtcgaacgggaggtataagagctgcctgcacagggcggtggtgaaccagcggcgggagcggcggtcgctggcgttcttcctgtgcccgcgggaggacagggtggtgcggccgccgccgagcgccgccacgccgcagcactacccggacttcacctgggccgacctcatgcgcttcacgcagcgccactaccgcgccgacacccgcacgctcgacgccttcacgcgctggctcgcgccgccggccgccgacgccgccgcgacggcgcaggtcgaggcggccagctga</cdnaseq>|<br />
AA = <aaseq>MVAEHPTPPQPHQPPPMDSTAGSGIAAPAAAAVCDLRMEPKIPE PFVWPNGDARPASAAELDMPVVDVGVLRDGDAEGLRRAAAQVAAACATHGFFQVSEHG VDAALARAALDGASDFFRLPLAEKRRARRVPGTVSGYTSAHADRFASKLPWKETLSFG FHDRAAAPVVADYFSSTLGPDFAPMGRVYQKYCEEMKELSLTIMELLELSLGVERGYY REFFADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDVGGLEVLVDGEWRP VSPVPGAMVINIGDTFMALSNGRYKSCLHRAVVNQRRERRSLAFFLCPREDRVVRPPP SAATPQHYPDFTWADLMRFTQRHYRADTRTLDAFTRWLAPPAADAAATAQVEAAS</aaseq>|<br />
DNA = <dnaseqindica>1..557#660..981#2453..2743#atggtggccgagcaccccacgccaccacagccgcaccaaccaccgcccatggactccaccgccggctctggcattgccgccccggcggcggcggcggtgtgcgacctgaggatggagcccaagatcccggagccattcgtgtggccgaacggcgacgcgaggccggcgtcggcggcggagctggacatgcccgtggtcgacgtgggcgtgctccgcgacggcgacgccgaggggctgcgccgcgccgcggcgcaggtggccgccgcgtgcgccacgcacgggttcttccaggtgtccgagcacggcgtcgacgccgctctggcgcgcgccgcgctcgacggcgccagcgacttcttccgcctcccgctcgccgagaagcgccgcgcgcgccgcgtcccgggcaccgtgtccggctacaccagcgcccacgccgaccgcttcgcctccaagctcccatggaaggagaccctctccttcggcttccacgaccgcgccgccgcccccgtcgtcgccgactacttctccagcaccctcggccccgacttcgcgccaatggggtaattaaaacgatggtggacgacattgcatttcaaattcaaaacaaattcaaaacacaccgaccgagattatgctgaattcaaacgcgtttgtgcgcgcaggagggtgtaccagaagtactgcgaggagatgaaggagctgtcgctgacgatcatggaactcctggagctgagcctgggcgtggagcgaggctactacagggagttcttcgcggacagcagctcaatcatgcggtgcaactactacccgccatgcccggagccggagcggacgctcggcacgggcccgcactgcgaccccaccgccctcaccatcctcctccaggacgacgtcggcggcctcgaggtcctcgtcgacggcgaatggcgccccgtcagccccgtccccggcgccatggtcatcaacatcggcgacaccttcatggtaaaccatctcctattctcctctcctctgttctcctctgcttcgaagcaacagaacaagtaattcaagcttttttttctctctcgcgcgaaattgacgagaaaaataagatcgtggtaggggcggggctttcagctgaaagcgggaagaaaccgacctgacgtgatttctctgttccaatcacaaacaatggaatgccccactcctccatgtgttatgatttatctcacatcttatagttaataggagtaagtaacaagctacttttttcatattatagttcgtttgattttttttttttaaagtttttttagttttatccaaatttattgaaaaacttagcaacgtttataataccaaattagtctcatttagtttaatattgtatatattttgataatatatttatgttatattaaaaatattactatatttttctataaacattattaaaagccatttataatataaaatggaaggagtaattaatatggatctcccccgacatgagaatattttccgatggtgtgacgacgccatgtaagcttcggtgggcctggacggccagaggtgccaacagccacgtccaacaacccctgggtccccccctaacactccaaacagtagtgagtagtgtctcgtcgcgttttagtatttgatgacaaacaaagtgtgagttgagttagccaccaccaacttgcacacgagcacatacatttgtgtccattctcgccagtcacttccatctctagtcctaactcctatctagcgatgtaagcggataatttcatcatccgtatataaacctgtttgttatagttaatttcctatataatactataacagtatacattttaaaagaaaacaaaattaggataaacaggccctgctcctatccatccatggcacttggaaggaccagactcggtcatgccatgccaagccaagatatgggttatggaagagtagagaagaggagagatgagagataagcatgcgttctcctcctcgttggatgtgtattttggagggatttgtgtagtagtagcagcggcgccgcggggacggatgcggatggtggcgctttcggtggcgttttcccgggggggttttggtttggcgcttgggggggatggcatggcgcggcgtgcggctgcacgccacacacacgcgcgcgcacgcacgtacgtcgtcgtcgccgcgggcggacggtagcttagggtggtgtgttccgcgcgcgggcgcggattgttccatgccgatcgatttggcgccaccctcgccgcggctcttgtcgcgtcgtgcgcctctctcgcgcggtttgtccttgtcgcgttgctcagccggcgacgggggcacggacattggcgatgtagccctgcacgtgtcggcctctccgttgatgaatgatgatgtatgtatgtatttttttttgtctgaaggaatttgtggggaattgttgtgtgtgcaggcgctgtcgaacgggaggtataagagctgcctgcacagggcggtggtgaaccagcggcgggagcggcggtcgctggcgttcttcctgtgcccgcgggaggacagggtggtgcggccgccgccgagcgccgccacgccgcagcactacccggacttcacctgggccgacctcatgcgcttcacgcagcgccactaccgcgccgacacccgcacgctcgacgccttcacgcgctggctcgcgccgccggccgccgacgccgccgcgacggcgcaggtcgaggcggccagctga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051549.1 RefSeq:Os01g0883800]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800/TimeDetail&diff=72841Os01g0883800/TimeDetail2012-07-14T21:04:47Z<p>Daweih: 1</p>
<hr />
<div><table border="1"><tr><th>Contributor</th><th>Edit Time</th></tr><tr><td>[[Special:ScienceReward|Lina Ma]]<br />
</td><td> 2012-07-12 18:52:58 | 2012-07-12 18:54:16 | 2012-07-12 18:56:29 | 2012-07-12 19:16:57 | 2012-07-12 19:24:50 | 2012-07-12 19:28:53 | 2012-07-12 19:36:54 | 2012-07-12 20:07:34 | 2012-07-12 20:36:30 | 2012-07-12 20:54:41 | 2012-07-12 23:36:21 | 2012-07-13 00:40:38 | 2012-07-13 01:02:33 | 2012-07-13 17:39:55 | 2012-07-14 01:09:09 | 2012-07-14 01:25:38 | 2012-07-14 01:27:34<br />
</td></tr><tr><td>[[Special:ScienceReward|Dawei Huang]]<br />
</td><td> 2012-07-06 20:02:20 | 2012-07-06 20:12:16 | 2012-07-06 20:16:00 | 2012-07-06 20:19:26 | 2012-07-06 20:24:50 | 2012-07-06 20:41:03 | 2012-07-13 06:03:25 | 2012-07-13 06:52:26 | 2012-07-13 06:58:33 | 2012-07-13 07:01:13 | 2012-07-15 04:58:39<br />
</td></tr><tr><td>[[Special:ScienceReward|Hao Wu]]<br />
</td><td> 2012-07-12 21:25:12<br />
</td></tr><tr><td>[[Special:ScienceReward|Rice2012]]<br />
</td><td> 2012-07-08 23:02:25 | 2012-07-09 02:52:45 | 2012-07-15 03:46:31<br />
</td></tr><tr><td>[[Special:ScienceReward|Chao Xu]]<br />
</td><td> 2012-07-12 22:08:24 | 2012-07-12 22:10:12 | 2012-07-12 22:17:49 | 2012-07-12 22:42:30<br />
</td></tr><tr><td>[[Special:ScienceReward|sangjian]]<br />
</td><td> 2012-07-06 20:09:27 | 2012-07-07 19:48:44 | 2012-07-07 19:52:41 | 2012-07-07 19:59:19 | 2012-07-07 20:02:51 | 2012-07-07 20:19:50 | 2012-07-07 20:26:03<br />
</td></tr></table><br />
Each contributor may involve multiple revisions, which are separated by '|'.</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800/QualityDetail&diff=72840Os01g0883800/QualityDetail2012-07-14T21:04:41Z<p>Daweih: 1</p>
<hr />
<div><table border="1"><tr><th>Contributor</th><th>Edit Quality</th></tr><tr><td>[[Special:ScienceReward|Lina Ma]]<br />
</td><td> -1 | -1 | 0.04 | 0.13 | -0.08 | -0.02 | -0.09 | 0.05 | -0.01 | 0.02 | -0.01 | 0.01 | 0.29 | 0.03 | 0.27 | 0.88 | 0.33<br />
</td></tr><tr><td>[[Special:ScienceReward|Dawei Huang]]<br />
</td><td> 0.14 | -0.54 | -0.15 | 0.01 | -0.15 | 0.79 | -0.12 | 0.26 | 0.18 | -0.16 | 1<br />
</td></tr><tr><td>[[Special:ScienceReward|Hao Wu]]<br />
</td><td> 0.49<br />
</td></tr><tr><td>[[Special:ScienceReward|Rice2012]]<br />
</td><td> -1 | 0.37 | -0.92<br />
</td></tr><tr><td>[[Special:ScienceReward|Chao Xu]]<br />
</td><td> -0.5 | -1 | -1 | -0.43<br />
</td></tr><tr><td>[[Special:ScienceReward|sangjian]]<br />
</td><td> 0.55 | -0.86 | 0.7 | -0.17 | -1 | -1 | -1<br />
</td></tr></table><br />
Each contributor may involve multiple revisions, which are separated by '|'.</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800/QuantityDetail&diff=72839Os01g0883800/QuantityDetail2012-07-14T21:04:36Z<p>Daweih: 1</p>
<hr />
<div><table border="1"><tr><th>Contributor</th><th>Edit Quantity</th></tr><tr><td>[[Special:ScienceReward|Lina Ma]]<br />
</td><td> 3984 | 17 | 2428 | 5114 | 2278 | 2800 | 60 | 1360 | 5777 | 4092 | 68 | 7604 | 6969 | 376 | 6991 | 6555 | 109<br />
</td></tr><tr><td>[[Special:ScienceReward|Dawei Huang]]<br />
</td><td> 4043 | 1071 | 585 | 180 | 240 | 3675 | 1973 | 5470 | 17 | 68 | 1950<br />
</td></tr><tr><td>[[Special:ScienceReward|Hao Wu]]<br />
</td><td> 6523<br />
</td></tr><tr><td>[[Special:ScienceReward|Rice2012]]<br />
</td><td> 25 | 1939 | 75<br />
</td></tr><tr><td>[[Special:ScienceReward|Chao Xu]]<br />
</td><td> 552 | 72 | 2 | 524<br />
</td></tr><tr><td>[[Special:ScienceReward|sangjian]]<br />
</td><td> 90 | 5992 | 6388 | 52 | 24 | 52 | 25<br />
</td></tr></table><br />
Each contributor may involve multiple revisions, which are separated by '|'.</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800&diff=72832Os01g08838002012-07-14T20:58:39Z<p>Daweih: </p>
<hr />
<div>The rice ''semidwarf-1 (sd1)'' is well known as the "green revolution gene" and controls the plant height of rice.<br />
<br />
== Annotated Information ==<br />
=== Function ===<br />
This gene is originally derived from the chinese cultivar Dee-Geo-Woo-Gen(DGWG). It encodes an oxidase enzyme involved in the biosynthesis of gibberellin, which is a plant growth hormone. The rice genome carries at least two GA20ox genes (GA20ox-1 and GA20ox-2).SD1 corresponds to GA20ox-2. Mutation of SD1 will cause a semi-dwarf phenotype of rice without seed yield being affected <ref name="ref1" />. It is not surprising that a rice semidwarfing gene encodes GA20-ox since successful production of semidwarf plants using antisense or overexpressed GA20-ox genes has been reported in Arabidopsis, Solanum dulcamara, potato, and lettuce <ref name="ref3" />.<br />
<br />
It is a key enzyme in the biosynthesis of gibberellin that catalyses the three steps GA53->GA44->GA19->GA20. Impaired GA 20-oxidase activity will cause elevated content of GA53, and reduced amount of G20 <ref name="ref1" />.<ref name="ref2" />. However, there is slight difference between different strains. The extent of GA1 is lower in Doongara (semi-dwarf rice strain) when compared with Kyeema (tall), while there is no significant difference between Calrose76 (semi-dwarf rice strain) and Calrose (tall). Calrose76 has lower contents of GA44 and of GA19 than Calrose, while there is no significant difference between Doongara (semi-dwarf rice strain) and Kyeema (tall) <ref name="ref2" />.<br />
<br />
GO asignment(s): GO:0005506, GO:0016216, GO:0017000<br />
<br />
=== Mutation ===<br />
‘dee-geo-woo-gen’ (semi-dwarf rice strain): A 383-base-pair deletion from the genome (A 280-bp deletion within the coding region), which induces a frameshift that creates a stop codon in SD1, may be related with the semi-dwarf phenotype <ref name="ref1" /><ref name="ref2" />.<br />
<br />
Calrose76 (semi-dwarf rice strain): The DNA sequence of Calrose76 is identical to Calrose (tall) except for a C to T transition at position 798 that resulted in a change of the predicted amino acid leucine (Leu-266) in Calrose to phenylalanine in Calrose76 <ref name="ref2" />.<br />
<br />
=== Expression ===<br />
This gene is strongly expressed in the leaf blade, stem and unopened flower, whereas GA20ox-1 is predominantly expressed in the unopened flower <ref name="ref1" />. <br />
<br />
Among the DGWG-type sd-1 mutants, IR24 and Habataki have little transcript of this gene, while Milyang 23 expresses a normal or greater amount of truncated transcript. No significant difference is observed between Calrose and its single-nucleotide-substitution mutant Calrose 76 <ref name="ref3" />.<br />
<br />
Analysis of the tissue- and stage-specificity of transcription of sd1 in Nipponbare revealed that this<br />
gene was expressed within 48 hr after sowing, as well as in 10-day-old plants, 30-day-old leaves, and flowering panicles; no transcription is detected in 24-hr-old seedlings or in 14-day-old roots. Transcript accumulates<br />
predominantly in adult leaves <ref name="ref3" />.<br />
<br />
Primers for gene amplication: forward primer, 5'-CAACTCACTCCCGCTCAACACAGC-3', and reverse primer, 5'-TTTGAAATGCAATGTCGTCCACC-3' were used to amplify exon 1; forward primer, 5'-GCGCCAATGGGGTAATTAAAACG-3' and reverse primer, 5'-GGCATTCCATTGTTTGTGATTGG-3' were used for exon 2; forward primer, 5'-GTTTGTCCTTGTCGCGTTGCTCAG-3' and reverse primer, 5'-TCTGTTCGTTCCGTTTCGTTCCG-3' were used for exon 3 <ref name="ref2" />.<br />
<br />
Primers for RT-PCR: forward primer 5'-CAACTCACTCCCGCTCAACACAGC- 3' located 22-bp upstream from the translation start, and a reverse primer 5'-GTTCGTTCCGTTTCGGTTCCG- 3' eight-bp downstream from the TGA stop codon <ref name="ref2" />.<br />
<br />
Primers for RT-PCR: forward primer: 5'-AGCTGGACATGCCCGTGGTC-3' and reverse primer : 5'-TTGAGCTGCTGTCCGCGAAG-3' <ref name="ref3" />.<br />
<br />
=== Evolution ===<br />
This gene is conserved in ''Arabidopsis'' (47% identity) and pea(50% identity). GA20ox-2 shows 47.8% identity to<br />
GA20ox-1 in rice. There are at least three GA20-ox genes in Arabidopsis <ref name="ref1" />.<ref name="ref2" />.<br />
<br />
=== Knowledge Extension ===<br />
The wheat green-revolution gene Rht (for ‘reduced height’)<ref name="ref4" /> is a gain-of-function allele caused by a mutation in a transcription factor that is associated with the gibberellin signalling pathway. As wheat has a hexaploid genome, it does not contain recessive alleles such as sd1 in rice that might otherwise be used to produce a semi-dwarf strain of wheat. Although the genetic and biochemical functions of the rice SD1 and wheat RHT proteins are completely different (that is, recessive versus dominant, loss-of-function versus gain-offunction events, enzyme versus transcription factor, respectively), the products of both genes are linked with gibberellin malfunction [1].<br />
<br />
== Labs working on this gene ==<br />
Bioscience Center, and Graduate School of Bioagricultural Science, Nagoya University, Nagoya 464-8601, Japan<br />
Honda R&D, Wako Research Center, Wako 351-0193, Japan<br />
<br />
International Rice Research Institute, Manila, DAPO Box 7777, Philippines<br />
<br />
BioResources Center, and Plant Molecular Biology Laboratory, Riken, Tsukuba 305-0074, Japan<br />
<br />
Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra ACT 2601, Australia<br />
<br />
Plant Genome Center, 1-25-2 Kannondai, Tsukuba, Ibaraki 305-0856, Japan<br />
<br />
== References ==<br />
WHERE CITE<ref name="ref3" />？<br />
<br />
<references><br />
<ref name="ref1">Sasaki A, Ashikari M, Ueguchi-Tanaka M, Itoh H, Nishimura A, et al. (2002) Green revolution: a mutant gibberellin-synthesis gene in rice. Nature 416: 701-702.</ref><br />
<ref name="ref2">Spielmeyer W, Ellis MH, Chandler PM (2002) Semidwarf (sd-1), "green revolution" rice, contains a defective gibberellin 20-oxidase gene. Proc Natl Acad Sci U S A 99: 9043-9048.</ref><br />
<ref name="ref3">Monna L, Kitazawa N, Yoshino R, Suzuki J, Masuda H, et al. (2002) Positional cloning of rice semidwarfing gene, sd-1: rice "green revolution gene" encodes a mutant enzyme involved in gibberellin synthesis. DNA Res 9: 11-17.</ref><br />
<ref name="ref4">Peng J, Richards DE, Hartley NM, Murphy GP, Devos KM, et al. (1999) 'Green revolution' genes encode mutant gibberellin response modulators. Nature 400: 256-261..</ref><br />
</references><br />
<br />
<br />
== Structured Information ==<br />
{{JaponicaGene|<br />
GeneName = Os01g0883800|<br />
Description = Gibberellin 20 oxidase 2 (EC 1.14.11.-) (Gibberellin C-20 oxidase 2) (GA 20-oxidase 2) (Os20ox2) (Semidwarf-1 protein)|<br />
Version = NM_001051549.1 GI:115441468 GeneID:4325003|<br />
Length = 2743 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0883800, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:40138313..40141055|<br />
CDS = 40138313..40138869,40138972..40139293,40140765..40141055|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:40138313..40141055<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:40138313..40141055<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggtggccgagcaccccacgccaccacagccgcaccaaccaccgcccatggactccaccgccggctctggcattgccgccccggcggcggcggcggtgtgcgacctgaggatggagcccaagatcccggagccattcgtgtggccgaacggcgacgcgaggccggcgtcggcggcggagctggacatgcccgtggtcgacgtgggcgtgctccgcgacggcgacgccgaggggctgcgccgcgccgcggcgcaggtggccgccgcgtgcgccacgcacgggttcttccaggtgtccgagcacggcgtcgacgccgctctggcgcgcgccgcgctcgacggcgccagcgacttcttccgcctcccgctcgccgagaagcgccgcgcgcgccgcgtcccgggcaccgtgtccggctacaccagcgcccacgccgaccgcttcgcctccaagctcccatggaaggagaccctctccttcggcttccacgaccgcgccgccgcccccgtcgtcgccgactacttctccagcaccctcggccccgacttcgcgccaatggggagggtgtaccagaagtactgcgaggagatgaaggagctgtcgctgacgatcatggaactcctggagctgagcctgggcgtggagcgaggctactacagggagttcttcgcggacagcagctcaatcatgcggtgcaactactacccgccatgcccggagccggagcggacgctcggcacgggcccgcactgcgaccccaccgccctcaccatcctcctccaggacgacgtcggcggcctcgaggtcctcgtcgacggcgaatggcgccccgtcagccccgtccccggcgccatggtcatcaacatcggcgacaccttcatggcgctgtcgaacgggaggtataagagctgcctgcacagggcggtggtgaaccagcggcgggagcggcggtcgctggcgttcttcctgtgcccgcgggaggacagggtggtgcggccgccgccgagcgccgccacgccgcagcactacccggacttcacctgggccgacctcatgcgcttcacgcagcgccactaccgcgccgacacccgcacgctcgacgccttcacgcgctggctcgcgccgccggccgccgacgccgccgcgacggcgcaggtcgaggcggccagctga</cdnaseq>|<br />
AA = <aaseq>MVAEHPTPPQPHQPPPMDSTAGSGIAAPAAAAVCDLRMEPKIPE PFVWPNGDARPASAAELDMPVVDVGVLRDGDAEGLRRAAAQVAAACATHGFFQVSEHG VDAALARAALDGASDFFRLPLAEKRRARRVPGTVSGYTSAHADRFASKLPWKETLSFG FHDRAAAPVVADYFSSTLGPDFAPMGRVYQKYCEEMKELSLTIMELLELSLGVERGYY REFFADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDVGGLEVLVDGEWRP VSPVPGAMVINIGDTFMALSNGRYKSCLHRAVVNQRRERRSLAFFLCPREDRVVRPPP SAATPQHYPDFTWADLMRFTQRHYRADTRTLDAFTRWLAPPAADAAATAQVEAAS</aaseq>|<br />
DNA = <dnaseqindica>1..557#660..981#2453..2743#atggtggccgagcaccccacgccaccacagccgcaccaaccaccgcccatggactccaccgccggctctggcattgccgccccggcggcggcggcggtgtgcgacctgaggatggagcccaagatcccggagccattcgtgtggccgaacggcgacgcgaggccggcgtcggcggcggagctggacatgcccgtggtcgacgtgggcgtgctccgcgacggcgacgccgaggggctgcgccgcgccgcggcgcaggtggccgccgcgtgcgccacgcacgggttcttccaggtgtccgagcacggcgtcgacgccgctctggcgcgcgccgcgctcgacggcgccagcgacttcttccgcctcccgctcgccgagaagcgccgcgcgcgccgcgtcccgggcaccgtgtccggctacaccagcgcccacgccgaccgcttcgcctccaagctcccatggaaggagaccctctccttcggcttccacgaccgcgccgccgcccccgtcgtcgccgactacttctccagcaccctcggccccgacttcgcgccaatggggtaattaaaacgatggtggacgacattgcatttcaaattcaaaacaaattcaaaacacaccgaccgagattatgctgaattcaaacgcgtttgtgcgcgcaggagggtgtaccagaagtactgcgaggagatgaaggagctgtcgctgacgatcatggaactcctggagctgagcctgggcgtggagcgaggctactacagggagttcttcgcggacagcagctcaatcatgcggtgcaactactacccgccatgcccggagccggagcggacgctcggcacgggcccgcactgcgaccccaccgccctcaccatcctcctccaggacgacgtcggcggcctcgaggtcctcgtcgacggcgaatggcgccccgtcagccccgtccccggcgccatggtcatcaacatcggcgacaccttcatggtaaaccatctcctattctcctctcctctgttctcctctgcttcgaagcaacagaacaagtaattcaagcttttttttctctctcgcgcgaaattgacgagaaaaataagatcgtggtaggggcggggctttcagctgaaagcgggaagaaaccgacctgacgtgatttctctgttccaatcacaaacaatggaatgccccactcctccatgtgttatgatttatctcacatcttatagttaataggagtaagtaacaagctacttttttcatattatagttcgtttgattttttttttttaaagtttttttagttttatccaaatttattgaaaaacttagcaacgtttataataccaaattagtctcatttagtttaatattgtatatattttgataatatatttatgttatattaaaaatattactatatttttctataaacattattaaaagccatttataatataaaatggaaggagtaattaatatggatctcccccgacatgagaatattttccgatggtgtgacgacgccatgtaagcttcggtgggcctggacggccagaggtgccaacagccacgtccaacaacccctgggtccccccctaacactccaaacagtagtgagtagtgtctcgtcgcgttttagtatttgatgacaaacaaagtgtgagttgagttagccaccaccaacttgcacacgagcacatacatttgtgtccattctcgccagtcacttccatctctagtcctaactcctatctagcgatgtaagcggataatttcatcatccgtatataaacctgtttgttatagttaatttcctatataatactataacagtatacattttaaaagaaaacaaaattaggataaacaggccctgctcctatccatccatggcacttggaaggaccagactcggtcatgccatgccaagccaagatatgggttatggaagagtagagaagaggagagatgagagataagcatgcgttctcctcctcgttggatgtgtattttggagggatttgtgtagtagtagcagcggcgccgcggggacggatgcggatggtggcgctttcggtggcgttttcccgggggggttttggtttggcgcttgggggggatggcatggcgcggcgtgcggctgcacgccacacacacgcgcgcgcacgcacgtacgtcgtcgtcgccgcgggcggacggtagcttagggtggtgtgttccgcgcgcgggcgcggattgttccatgccgatcgatttggcgccaccctcgccgcggctcttgtcgcgtcgtgcgcctctctcgcgcggtttgtccttgtcgcgttgctcagccggcgacgggggcacggacattggcgatgtagccctgcacgtgtcggcctctccgttgatgaatgatgatgtatgtatgtatttttttttgtctgaaggaatttgtggggaattgttgtgtgtgcaggcgctgtcgaacgggaggtataagagctgcctgcacagggcggtggtgaaccagcggcgggagcggcggtcgctggcgttcttcctgtgcccgcgggaggacagggtggtgcggccgccgccgagcgccgccacgccgcagcactacccggacttcacctgggccgacctcatgcgcttcacgcagcgccactaccgcgccgacacccgcacgctcgacgccttcacgcgctggctcgcgccgccggccgccgacgccgccgcgacggcgcaggtcgaggcggccagctga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051549.1 RefSeq:Os01g0883800]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Talk:Os01g0883800&diff=69950Talk:Os01g08838002012-07-12T23:16:09Z<p>Daweih: /* NO citation of reference */ new section</p>
<hr />
<div>Hi Guys,<br />
<br />
I suggest the information should be grouped into different sections, similar with the following.<br />
<br />
1. One sentence summary<br />
<br />
2. Annotated details<br />
<br />
2.1 sub-section 1<br />
2.2 sub-section 2<br />
......<br />
2.n sub-section n<br />
<br />
3. Structured information<br />
Template table<br />
<br />
Here is my two cents. Any other ideas or comments?<br />
<br />
ZZ<br />
<br />
== NO citation of reference ==<br />
<br />
In the main body of the text, there is no place cite ref3. Please fix this. Thanks!</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800/TimeDetail&diff=69940Os01g0883800/TimeDetail2012-07-12T23:02:31Z<p>Daweih: 1</p>
<hr />
<div><table border="1"><tr><th>Contributor</th><th>Edit Time</th></tr><tr><td>[[Special:ScienceReward|Dawei Huang]]<br />
</td><td> 2012-07-06 20:02:20 | 2012-07-06 20:12:16 | 2012-07-06 20:16:00 | 2012-07-06 20:19:26 | 2012-07-06 20:24:50 | 2012-07-06 20:41:03 | 2012-07-13 06:03:25 | 2012-07-13 06:52:26 | 2012-07-13 06:58:33 | 2012-07-13 07:01:13<br />
</td></tr><tr><td>[[Special:ScienceReward|Hao Wu]]<br />
</td><td> 2012-07-12 21:25:12<br />
</td></tr><tr><td>[[Special:ScienceReward|Rice2012]]<br />
</td><td> 2012-07-08 23:02:25 | 2012-07-09 02:52:45<br />
</td></tr><tr><td>[[Special:ScienceReward|Lina Ma]]<br />
</td><td> 2012-07-12 18:52:58 | 2012-07-12 18:54:16 | 2012-07-12 18:56:29 | 2012-07-12 19:16:57 | 2012-07-12 19:24:50 | 2012-07-12 19:28:53 | 2012-07-12 19:36:54 | 2012-07-12 20:07:34 | 2012-07-12 20:36:30 | 2012-07-12 20:54:41 | 2012-07-12 23:36:21 | 2012-07-13 00:40:38 | 2012-07-13 01:02:33<br />
</td></tr><tr><td>[[Special:ScienceReward|Chao Xu]]<br />
</td><td> 2012-07-12 22:08:24 | 2012-07-12 22:10:12 | 2012-07-12 22:17:49 | 2012-07-12 22:42:30<br />
</td></tr><tr><td>[[Special:ScienceReward|sangjian]]<br />
</td><td> 2012-07-06 20:09:27 | 2012-07-07 19:48:44 | 2012-07-07 19:52:41 | 2012-07-07 19:59:19 | 2012-07-07 20:02:51 | 2012-07-07 20:19:50 | 2012-07-07 20:26:03<br />
</td></tr></table><br />
Each contributor may involve multiple revisions, which are separated by '|'.</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800/QualityDetail&diff=69939Os01g0883800/QualityDetail2012-07-12T23:02:27Z<p>Daweih: 1</p>
<hr />
<div><table border="1"><tr><th>Contributor</th><th>Edit Quality</th></tr><tr><td>[[Special:ScienceReward|Dawei Huang]]<br />
</td><td> 0.14 | -0.54 | -0.15 | 0.01 | -0.15 | 0.79 | 1 | 1 | 1 | 1<br />
</td></tr><tr><td>[[Special:ScienceReward|Hao Wu]]<br />
</td><td> 0.49<br />
</td></tr><tr><td>[[Special:ScienceReward|Rice2012]]<br />
</td><td> -1 | 0.37<br />
</td></tr><tr><td>[[Special:ScienceReward|Lina Ma]]<br />
</td><td> -1 | -1 | 0.04 | 0.13 | -0.08 | -0.02 | -0.09 | 0.05 | -0.01 | 0.02 | -0.01 | 0.05 | 0.43<br />
</td></tr><tr><td>[[Special:ScienceReward|Chao Xu]]<br />
</td><td> -0.5 | 0.16 | 0.14 | 0.13<br />
</td></tr><tr><td>[[Special:ScienceReward|sangjian]]<br />
</td><td> 0.55 | -0.86 | 0.7 | -0.17 | -1 | -1 | -1<br />
</td></tr></table><br />
Each contributor may involve multiple revisions, which are separated by '|'.</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800/QuantityDetail&diff=69938Os01g0883800/QuantityDetail2012-07-12T23:02:22Z<p>Daweih: 1</p>
<hr />
<div><table border="1"><tr><th>Contributor</th><th>Edit Quantity</th></tr><tr><td>[[Special:ScienceReward|Dawei Huang]]<br />
</td><td> 4043 | 1071 | 585 | 180 | 240 | 3675 | 1973 | 5470 | 17 | 68<br />
</td></tr><tr><td>[[Special:ScienceReward|Hao Wu]]<br />
</td><td> 6523<br />
</td></tr><tr><td>[[Special:ScienceReward|Rice2012]]<br />
</td><td> 25 | 1939<br />
</td></tr><tr><td>[[Special:ScienceReward|Lina Ma]]<br />
</td><td> 3984 | 17 | 2428 | 5114 | 2278 | 2800 | 60 | 1360 | 5777 | 4092 | 68 | 7604 | 6969<br />
</td></tr><tr><td>[[Special:ScienceReward|Chao Xu]]<br />
</td><td> 552 | 72 | 2 | 524<br />
</td></tr><tr><td>[[Special:ScienceReward|sangjian]]<br />
</td><td> 90 | 5992 | 6388 | 52 | 24 | 52 | 25<br />
</td></tr></table><br />
Each contributor may involve multiple revisions, which are separated by '|'.</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800&diff=69937Os01g08838002012-07-12T23:01:13Z<p>Daweih: </p>
<hr />
<div>The rice ''semidwarf-1 (sd1)'' is well known as the "green revolution gene" and controls the plant height of rice.<br />
<br />
== Annotated Information ==<br />
=== Function ===<br />
This gene is originally derived from the chinese cultivar Dee-Geo-Woo-Gen(DGWG). It encodes an oxidase enzyme involved in the biosynthesis of gibberellin, which is a plant growth hormone. The rice genome carries at least two GA20ox genes (GA20ox-1 and GA20ox-2).SD1 corresponds to GA20ox-2. Mutation of SD1 will cause a semi-dwarf phenotype of rice without seed yield being affected <ref name="ref1" />. It is not surprising that a rice semidwarfing gene encodes GA20-ox since successful production of semidwarf plants using antisense or overexpressed GA20-ox genes has been reported in Arabidopsis, Solanum dulcamara, potato, and lettuce.<br />
<br />
It is a key enzyme in the biosynthesis of gibberellin that catalyses the three steps GA53->GA44->GA19->GA20. Impaired GA 20-oxidase activity will cause elevated content of GA53, and reduced amount of G20 <ref name="ref1" />.<ref name="ref2" />. However, there is slight difference between different strains. The extent of GA1 is lower in Doongara (semi-dwarf rice strain) when compared with Kyeema (tall), while there is no significant difference between Calrose76 (semi-dwarf rice strain) and Calrose (tall). Calrose76 has lower contents of GA44 and of GA19 than Calrose, while there is no significant difference between Doongara (semi-dwarf rice strain) when compared with Kyeema (tall) <ref name="ref2" />.<br />
<br />
GO asignment(s): GO:0005506, GO:0016216, GO:0017000<br />
<br />
=== Mutation ===<br />
‘dee-geo-woo-gen’ (semi-dwarf rice strain) and IR8 (semi-dwarf rice strain): A 383-base-pair deletion, which induces a frameshift that creates a stop codon in SD1 may be related with the semi-dwarf phenotype <ref name="ref1" />. <br />
<br />
Doongara (semi-dwarf rice strain): A 280-bp deletion within the coding region, which spans parts of exon 1 and exon 2, is predicted to result in a truncated, nonfunctional polypeptide containing the first 99 amino acid residues of the GA 2-oxidase, followed by an additional 3 amino acids that are in the wrong reading frame before a termination codon <ref name="ref2" />.<br />
<br />
Calrose76 (semi-dwarf rice strain): The DNA sequence of Calrose76 is identical to Calrose (tall) except for a C to T transition at position 798 that resulted in a change of the predicted amino acid leucine (Leu-266) in Calrose to phenylalanine in Calrose76 <ref name="ref2" />.<br />
<br />
<br />
<br />
=== Expression ===<br />
This gene is strongly expressed in the leaf blade, stem and unopened flower, whereas GA20ox-1 is predominantly expressed in the unopened flower <ref name="ref1" />.<br />
<br />
Primers for DNA amplication: forward primer, 5'-CAACTCACTCCCGCTCAACACAGC-3', and reverse primer, 5'-TTTGAAATGCAATGTCGTCCACC-3' were used to amplify exon 1; forward primer, 5'-GCGCCAATGGGGTAATTAAAACG-3' and reverse primer, 5'-GGCATTCCATTGTTTGTGATTGG-3' were used for exon 2; forward primer, 5'-GTTTGTCCTTGTCGCGTTGCTCAG-3' and reverse primer, 5'-TCTGTTCGTTCCGTTTCGTTCCG-3' were used for exon 3 <ref name="ref2" />.<br />
<br />
Primers for RT-PCR: forward primer 5'-CAACTCACTCCCGCTCAACACAGC- 3' located 22-bp upstream from the translation start, and a reverse primer 5'-GTTCGTTCCGTTTCGGTTCCG- 3' eight-bp downstream from the TGA stop codon <ref name="ref2" />.<br />
<br />
=== Evolution ===<br />
This gene is conserved in ''Arabidopsis'' (47% identity) and pea(50% identity). GA20ox-2 shows 47.8% identity to<br />
GA20ox-1 in rice. There are at least three GA20-ox genes in Arabidopsis <ref name="ref1" />.<ref name="ref2" />.<br />
<br />
== Labs working on this gene ==<br />
Bioscience Center, and Graduate School of Bioagricultural Science, Nagoya University, Nagoya 464-8601, Japan<br />
Honda R&D, Wako Research Center, Wako 351-0193, Japan<br />
<br />
International Rice Research Institute, Manila, DAPO Box 7777, Philippines<br />
<br />
BioResources Center, and Plant Molecular Biology Laboratory, Riken, Tsukuba 305-0074, Japan<br />
<br />
Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra ACT 2601, Australia<br />
<br />
Plant Genome Center, 1-25-2 Kannondai, Tsukuba, Ibaraki 305-0856, Japan<br />
<br />
== References ==<br />
WHERE CITE<ref name="ref3" />？<br />
<br />
<references><br />
<ref name="ref1">Sasaki A, Ashikari M, Ueguchi-Tanaka M, Itoh H, Nishimura A, et al. (2002) Green revolution: a mutant gibberellin-synthesis gene in rice. Nature 416: 701-702.</ref><br />
<ref name="ref2">Spielmeyer W, Ellis MH, Chandler PM (2002) Semidwarf (sd-1), "green revolution" rice, contains a defective gibberellin 20-oxidase gene. Proc Natl Acad Sci U S A 99: 9043-9048.</ref><br />
<ref name="ref3">Monna L, Kitazawa N, Yoshino R, Suzuki J, Masuda H, et al. (2002) Positional cloning of rice semidwarfing gene, sd-1: rice "green revolution gene" encodes a mutant enzyme involved in gibberellin synthesis. DNA Res 9: 11-17.</ref><br />
</references><br />
<br />
<br />
== Structured Information ==<br />
{{JaponicaGene|<br />
GeneName = Os01g0883800|<br />
Description = Gibberellin 20 oxidase 2 (EC 1.14.11.-) (Gibberellin C-20 oxidase 2) (GA 20-oxidase 2) (Os20ox2) (Semidwarf-1 protein)|<br />
Version = NM_001051549.1 GI:115441468 GeneID:4325003|<br />
Length = 2743 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0883800, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:40138313..40141055|<br />
CDS = 40138313..40138869,40138972..40139293,40140765..40141055|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:40138313..40141055<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:40138313..40141055<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggtggccgagcaccccacgccaccacagccgcaccaaccaccgcccatggactccaccgccggctctggcattgccgccccggcggcggcggcggtgtgcgacctgaggatggagcccaagatcccggagccattcgtgtggccgaacggcgacgcgaggccggcgtcggcggcggagctggacatgcccgtggtcgacgtgggcgtgctccgcgacggcgacgccgaggggctgcgccgcgccgcggcgcaggtggccgccgcgtgcgccacgcacgggttcttccaggtgtccgagcacggcgtcgacgccgctctggcgcgcgccgcgctcgacggcgccagcgacttcttccgcctcccgctcgccgagaagcgccgcgcgcgccgcgtcccgggcaccgtgtccggctacaccagcgcccacgccgaccgcttcgcctccaagctcccatggaaggagaccctctccttcggcttccacgaccgcgccgccgcccccgtcgtcgccgactacttctccagcaccctcggccccgacttcgcgccaatggggagggtgtaccagaagtactgcgaggagatgaaggagctgtcgctgacgatcatggaactcctggagctgagcctgggcgtggagcgaggctactacagggagttcttcgcggacagcagctcaatcatgcggtgcaactactacccgccatgcccggagccggagcggacgctcggcacgggcccgcactgcgaccccaccgccctcaccatcctcctccaggacgacgtcggcggcctcgaggtcctcgtcgacggcgaatggcgccccgtcagccccgtccccggcgccatggtcatcaacatcggcgacaccttcatggcgctgtcgaacgggaggtataagagctgcctgcacagggcggtggtgaaccagcggcgggagcggcggtcgctggcgttcttcctgtgcccgcgggaggacagggtggtgcggccgccgccgagcgccgccacgccgcagcactacccggacttcacctgggccgacctcatgcgcttcacgcagcgccactaccgcgccgacacccgcacgctcgacgccttcacgcgctggctcgcgccgccggccgccgacgccgccgcgacggcgcaggtcgaggcggccagctga</cdnaseq>|<br />
AA = <aaseq>MVAEHPTPPQPHQPPPMDSTAGSGIAAPAAAAVCDLRMEPKIPE PFVWPNGDARPASAAELDMPVVDVGVLRDGDAEGLRRAAAQVAAACATHGFFQVSEHG VDAALARAALDGASDFFRLPLAEKRRARRVPGTVSGYTSAHADRFASKLPWKETLSFG FHDRAAAPVVADYFSSTLGPDFAPMGRVYQKYCEEMKELSLTIMELLELSLGVERGYY REFFADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDVGGLEVLVDGEWRP VSPVPGAMVINIGDTFMALSNGRYKSCLHRAVVNQRRERRSLAFFLCPREDRVVRPPP SAATPQHYPDFTWADLMRFTQRHYRADTRTLDAFTRWLAPPAADAAATAQVEAAS</aaseq>|<br />
DNA = <dnaseqindica>1..557#660..981#2453..2743#atggtggccgagcaccccacgccaccacagccgcaccaaccaccgcccatggactccaccgccggctctggcattgccgccccggcggcggcggcggtgtgcgacctgaggatggagcccaagatcccggagccattcgtgtggccgaacggcgacgcgaggccggcgtcggcggcggagctggacatgcccgtggtcgacgtgggcgtgctccgcgacggcgacgccgaggggctgcgccgcgccgcggcgcaggtggccgccgcgtgcgccacgcacgggttcttccaggtgtccgagcacggcgtcgacgccgctctggcgcgcgccgcgctcgacggcgccagcgacttcttccgcctcccgctcgccgagaagcgccgcgcgcgccgcgtcccgggcaccgtgtccggctacaccagcgcccacgccgaccgcttcgcctccaagctcccatggaaggagaccctctccttcggcttccacgaccgcgccgccgcccccgtcgtcgccgactacttctccagcaccctcggccccgacttcgcgccaatggggtaattaaaacgatggtggacgacattgcatttcaaattcaaaacaaattcaaaacacaccgaccgagattatgctgaattcaaacgcgtttgtgcgcgcaggagggtgtaccagaagtactgcgaggagatgaaggagctgtcgctgacgatcatggaactcctggagctgagcctgggcgtggagcgaggctactacagggagttcttcgcggacagcagctcaatcatgcggtgcaactactacccgccatgcccggagccggagcggacgctcggcacgggcccgcactgcgaccccaccgccctcaccatcctcctccaggacgacgtcggcggcctcgaggtcctcgtcgacggcgaatggcgccccgtcagccccgtccccggcgccatggtcatcaacatcggcgacaccttcatggtaaaccatctcctattctcctctcctctgttctcctctgcttcgaagcaacagaacaagtaattcaagcttttttttctctctcgcgcgaaattgacgagaaaaataagatcgtggtaggggcggggctttcagctgaaagcgggaagaaaccgacctgacgtgatttctctgttccaatcacaaacaatggaatgccccactcctccatgtgttatgatttatctcacatcttatagttaataggagtaagtaacaagctacttttttcatattatagttcgtttgattttttttttttaaagtttttttagttttatccaaatttattgaaaaacttagcaacgtttataataccaaattagtctcatttagtttaatattgtatatattttgataatatatttatgttatattaaaaatattactatatttttctataaacattattaaaagccatttataatataaaatggaaggagtaattaatatggatctcccccgacatgagaatattttccgatggtgtgacgacgccatgtaagcttcggtgggcctggacggccagaggtgccaacagccacgtccaacaacccctgggtccccccctaacactccaaacagtagtgagtagtgtctcgtcgcgttttagtatttgatgacaaacaaagtgtgagttgagttagccaccaccaacttgcacacgagcacatacatttgtgtccattctcgccagtcacttccatctctagtcctaactcctatctagcgatgtaagcggataatttcatcatccgtatataaacctgtttgttatagttaatttcctatataatactataacagtatacattttaaaagaaaacaaaattaggataaacaggccctgctcctatccatccatggcacttggaaggaccagactcggtcatgccatgccaagccaagatatgggttatggaagagtagagaagaggagagatgagagataagcatgcgttctcctcctcgttggatgtgtattttggagggatttgtgtagtagtagcagcggcgccgcggggacggatgcggatggtggcgctttcggtggcgttttcccgggggggttttggtttggcgcttgggggggatggcatggcgcggcgtgcggctgcacgccacacacacgcgcgcgcacgcacgtacgtcgtcgtcgccgcgggcggacggtagcttagggtggtgtgttccgcgcgcgggcgcggattgttccatgccgatcgatttggcgccaccctcgccgcggctcttgtcgcgtcgtgcgcctctctcgcgcggtttgtccttgtcgcgttgctcagccggcgacgggggcacggacattggcgatgtagccctgcacgtgtcggcctctccgttgatgaatgatgatgtatgtatgtatttttttttgtctgaaggaatttgtggggaattgttgtgtgtgcaggcgctgtcgaacgggaggtataagagctgcctgcacagggcggtggtgaaccagcggcgggagcggcggtcgctggcgttcttcctgtgcccgcgggaggacagggtggtgcggccgccgccgagcgccgccacgccgcagcactacccggacttcacctgggccgacctcatgcgcttcacgcagcgccactaccgcgccgacacccgcacgctcgacgccttcacgcgctggctcgcgccgccggccgccgacgccgccgcgacggcgcaggtcgaggcggccagctga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051549.1 RefSeq:Os01g0883800]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800/TimeDetail&diff=69936Os01g0883800/TimeDetail2012-07-12T22:59:55Z<p>Daweih: 1</p>
<hr />
<div><table border="1"><tr><th>Contributor</th><th>Edit Time</th></tr><tr><td>[[Special:ScienceReward|Dawei Huang]]<br />
</td><td> 2012-07-06 20:02:20 | 2012-07-06 20:12:16 | 2012-07-06 20:16:00 | 2012-07-06 20:19:26 | 2012-07-06 20:24:50 | 2012-07-06 20:41:03 | 2012-07-13 06:03:25 | 2012-07-13 06:52:26 | 2012-07-13 06:58:33<br />
</td></tr><tr><td>[[Special:ScienceReward|Hao Wu]]<br />
</td><td> 2012-07-12 21:25:12<br />
</td></tr><tr><td>[[Special:ScienceReward|Rice2012]]<br />
</td><td> 2012-07-08 23:02:25 | 2012-07-09 02:52:45<br />
</td></tr><tr><td>[[Special:ScienceReward|Lina Ma]]<br />
</td><td> 2012-07-12 18:52:58 | 2012-07-12 18:54:16 | 2012-07-12 18:56:29 | 2012-07-12 19:16:57 | 2012-07-12 19:24:50 | 2012-07-12 19:28:53 | 2012-07-12 19:36:54 | 2012-07-12 20:07:34 | 2012-07-12 20:36:30 | 2012-07-12 20:54:41 | 2012-07-12 23:36:21 | 2012-07-13 00:40:38 | 2012-07-13 01:02:33<br />
</td></tr><tr><td>[[Special:ScienceReward|Chao Xu]]<br />
</td><td> 2012-07-12 22:08:24 | 2012-07-12 22:10:12 | 2012-07-12 22:17:49 | 2012-07-12 22:42:30<br />
</td></tr><tr><td>[[Special:ScienceReward|sangjian]]<br />
</td><td> 2012-07-06 20:09:27 | 2012-07-07 19:48:44 | 2012-07-07 19:52:41 | 2012-07-07 19:59:19 | 2012-07-07 20:02:51 | 2012-07-07 20:19:50 | 2012-07-07 20:26:03<br />
</td></tr></table><br />
Each contributor may involve multiple revisions, which are separated by '|'.</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800/QualityDetail&diff=69935Os01g0883800/QualityDetail2012-07-12T22:59:51Z<p>Daweih: 1</p>
<hr />
<div><table border="1"><tr><th>Contributor</th><th>Edit Quality</th></tr><tr><td>[[Special:ScienceReward|Dawei Huang]]<br />
</td><td> 0.14 | -0.54 | -0.15 | 0.01 | -0.15 | 0.79 | 1 | 1 | 1<br />
</td></tr><tr><td>[[Special:ScienceReward|Hao Wu]]<br />
</td><td> 0.49<br />
</td></tr><tr><td>[[Special:ScienceReward|Rice2012]]<br />
</td><td> -1 | 0.37<br />
</td></tr><tr><td>[[Special:ScienceReward|Lina Ma]]<br />
</td><td> -1 | -1 | 0.04 | 0.13 | -0.08 | -0.02 | -0.09 | 0.05 | -0.01 | 0.02 | 0.01 | 0.06 | 0.47<br />
</td></tr><tr><td>[[Special:ScienceReward|Chao Xu]]<br />
</td><td> -0.16 | 0.18 | 0.17 | 0.15<br />
</td></tr><tr><td>[[Special:ScienceReward|sangjian]]<br />
</td><td> 0.55 | -0.86 | 0.7 | -0.17 | -1 | -1 | -1<br />
</td></tr></table><br />
Each contributor may involve multiple revisions, which are separated by '|'.</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800/QuantityDetail&diff=69934Os01g0883800/QuantityDetail2012-07-12T22:59:47Z<p>Daweih: 1</p>
<hr />
<div><table border="1"><tr><th>Contributor</th><th>Edit Quantity</th></tr><tr><td>[[Special:ScienceReward|Dawei Huang]]<br />
</td><td> 4043 | 1071 | 585 | 180 | 240 | 3675 | 1973 | 5470 | 17<br />
</td></tr><tr><td>[[Special:ScienceReward|Hao Wu]]<br />
</td><td> 6523<br />
</td></tr><tr><td>[[Special:ScienceReward|Rice2012]]<br />
</td><td> 25 | 1939<br />
</td></tr><tr><td>[[Special:ScienceReward|Lina Ma]]<br />
</td><td> 3984 | 17 | 2428 | 5114 | 2278 | 2800 | 60 | 1360 | 5777 | 4092 | 68 | 7604 | 6969<br />
</td></tr><tr><td>[[Special:ScienceReward|Chao Xu]]<br />
</td><td> 552 | 72 | 2 | 524<br />
</td></tr><tr><td>[[Special:ScienceReward|sangjian]]<br />
</td><td> 90 | 5992 | 6388 | 52 | 24 | 52 | 25<br />
</td></tr></table><br />
Each contributor may involve multiple revisions, which are separated by '|'.</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800&diff=69933Os01g08838002012-07-12T22:58:33Z<p>Daweih: </p>
<hr />
<div>The rice ''semidwarf-1 (sd1)'' is well known as the "green revolution gene" and controls the plant height of rice.<br />
<br />
== Annotated Information ==<br />
=== Function ===<br />
This gene is originally derived from the chinese cultivar Dee-Geo-Woo-Gen(DGWG). It encodes an oxidase enzyme involved in the biosynthesis of gibberellin, which is a plant growth hormone. The rice genome carries at least two GA20ox genes (GA20ox-1 and GA20ox-2).SD1 corresponds to GA20ox-2. Mutation of SD1 will cause a semi-dwarf phenotype of rice without seed yield being affected <ref name="ref1" />. It is not surprising that a rice semidwarfing gene encodes GA20-ox since successful production of semidwarf plants using antisense or overexpressed GA20-ox genes has been reported in Arabidopsis, Solanum dulcamara, potato, and lettuce.<br />
<br />
It is a key enzyme in the biosynthesis of gibberellin that catalyses the three steps GA53->GA44->GA19->GA20. Impaired GA 20-oxidase activity will cause elevated content of GA53, and reduced amount of G20 <ref name="ref1" />.<ref name="ref2" />. However, there is slight difference between different strains. The extent of GA1 is lower in Doongara (semi-dwarf rice strain) when compared with Kyeema (tall), while there is no significant difference between Calrose76 (semi-dwarf rice strain) and Calrose (tall). Calrose76 has lower contents of GA44 and of GA19 than Calrose, while there is no significant difference between Doongara (semi-dwarf rice strain) when compared with Kyeema (tall) <ref name="ref2" />.<br />
<br />
GO asignment(s): GO:0005506, GO:0016216, GO:0017000<br />
<br />
=== Mutation ===<br />
‘dee-geo-woo-gen’ (semi-dwarf rice strain) and IR8 (semi-dwarf rice strain): A 383-base-pair deletion, which induces a frameshift that creates a stop codon in SD1 may be related with the semi-dwarf phenotype <ref name="ref1" />. <br />
<br />
Doongara (semi-dwarf rice strain): A 280-bp deletion within the coding region, which spans parts of exon 1 and exon 2, is predicted to result in a truncated, nonfunctional polypeptide containing the first 99 amino acid residues of the GA 2-oxidase, followed by an additional 3 amino acids that are in the wrong reading frame before a termination codon <ref name="ref2" />.<br />
<br />
Calrose76 (semi-dwarf rice strain): The DNA sequence of Calrose76 is identical to Calrose (tall) except for a C to T transition at position 798 that resulted in a change of the predicted amino acid leucine (Leu-266) in Calrose to phenylalanine in Calrose76 <ref name="ref2" />.<br />
<br />
<br />
<br />
=== Expression ===<br />
This gene is strongly expressed in the leaf blade, stem and unopened flower, whereas GA20ox-1 is predominantly expressed in the unopened flower <ref name="ref1" />.<br />
<br />
Primers for DNA amplication: forward primer, 5'-CAACTCACTCCCGCTCAACACAGC-3', and reverse primer, 5'-TTTGAAATGCAATGTCGTCCACC-3' were used to amplify exon 1; forward primer, 5'-GCGCCAATGGGGTAATTAAAACG-3' and reverse primer, 5'-GGCATTCCATTGTTTGTGATTGG-3' were used for exon 2; forward primer, 5'-GTTTGTCCTTGTCGCGTTGCTCAG-3' and reverse primer, 5'-TCTGTTCGTTCCGTTTCGTTCCG-3' were used for exon 3 <ref name="ref2" />.<br />
<br />
Primers for RT-PCR: forward primer 5'-CAACTCACTCCCGCTCAACACAGC- 3' located 22-bp upstream from the translation start, and a reverse primer 5'-GTTCGTTCCGTTTCGGTTCCG- 3' eight-bp downstream from the TGA stop codon <ref name="ref2" />.<br />
<br />
=== Evolution ===<br />
This gene is conserved in ''Arabidopsis'' (47% identity) and pea(50% identity). GA20ox-2 shows 47.8% identity to<br />
GA20ox-1 in rice. There are at least three GA20-ox genes in Arabidopsis <ref name="ref1" />.<ref name="ref2" />.<br />
<br />
== Labs working on this gene ==<br />
Bioscience Center, and Graduate School of Bioagricultural Science, Nagoya University, Nagoya 464-8601, Japan<br />
Honda R&D, Wako Research Center, Wako 351-0193, Japan<br />
<br />
International Rice Research Institute, Manila, DAPO Box 7777, Philippines<br />
<br />
BioResources Center, and Plant Molecular Biology Laboratory, Riken, Tsukuba 305-0074, Japan<br />
<br />
Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra ACT 2601, Australia<br />
<br />
Plant Genome Center, 1-25-2 Kannondai, Tsukuba, Ibaraki 305-0856, Japan<br />
<br />
== References ==<br />
WHERE CITE<ref name="ref3" /><br />
<references><br />
<ref name="ref1">Sasaki A, Ashikari M, Ueguchi-Tanaka M, Itoh H, Nishimura A, et al. (2002) Green revolution: a mutant gibberellin-synthesis gene in rice. Nature 416: 701-702.</ref><br />
<ref name="ref2">Spielmeyer W, Ellis MH, Chandler PM (2002) Semidwarf (sd-1), "green revolution" rice, contains a defective gibberellin 20-oxidase gene. Proc Natl Acad Sci U S A 99: 9043-9048.</ref><br />
<ref name="ref3">Monna L, Kitazawa N, Yoshino R, Suzuki J, Masuda H, et al. (2002) Positional cloning of rice semidwarfing gene, sd-1: rice "green revolution gene" encodes a mutant enzyme involved in gibberellin synthesis. DNA Res 9: 11-17.</ref><br />
</references><br />
<br />
<br />
== Structured Information ==<br />
{{JaponicaGene|<br />
GeneName = Os01g0883800|<br />
Description = Gibberellin 20 oxidase 2 (EC 1.14.11.-) (Gibberellin C-20 oxidase 2) (GA 20-oxidase 2) (Os20ox2) (Semidwarf-1 protein)|<br />
Version = NM_001051549.1 GI:115441468 GeneID:4325003|<br />
Length = 2743 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0883800, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:40138313..40141055|<br />
CDS = 40138313..40138869,40138972..40139293,40140765..40141055|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:40138313..40141055<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:40138313..40141055<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggtggccgagcaccccacgccaccacagccgcaccaaccaccgcccatggactccaccgccggctctggcattgccgccccggcggcggcggcggtgtgcgacctgaggatggagcccaagatcccggagccattcgtgtggccgaacggcgacgcgaggccggcgtcggcggcggagctggacatgcccgtggtcgacgtgggcgtgctccgcgacggcgacgccgaggggctgcgccgcgccgcggcgcaggtggccgccgcgtgcgccacgcacgggttcttccaggtgtccgagcacggcgtcgacgccgctctggcgcgcgccgcgctcgacggcgccagcgacttcttccgcctcccgctcgccgagaagcgccgcgcgcgccgcgtcccgggcaccgtgtccggctacaccagcgcccacgccgaccgcttcgcctccaagctcccatggaaggagaccctctccttcggcttccacgaccgcgccgccgcccccgtcgtcgccgactacttctccagcaccctcggccccgacttcgcgccaatggggagggtgtaccagaagtactgcgaggagatgaaggagctgtcgctgacgatcatggaactcctggagctgagcctgggcgtggagcgaggctactacagggagttcttcgcggacagcagctcaatcatgcggtgcaactactacccgccatgcccggagccggagcggacgctcggcacgggcccgcactgcgaccccaccgccctcaccatcctcctccaggacgacgtcggcggcctcgaggtcctcgtcgacggcgaatggcgccccgtcagccccgtccccggcgccatggtcatcaacatcggcgacaccttcatggcgctgtcgaacgggaggtataagagctgcctgcacagggcggtggtgaaccagcggcgggagcggcggtcgctggcgttcttcctgtgcccgcgggaggacagggtggtgcggccgccgccgagcgccgccacgccgcagcactacccggacttcacctgggccgacctcatgcgcttcacgcagcgccactaccgcgccgacacccgcacgctcgacgccttcacgcgctggctcgcgccgccggccgccgacgccgccgcgacggcgcaggtcgaggcggccagctga</cdnaseq>|<br />
AA = <aaseq>MVAEHPTPPQPHQPPPMDSTAGSGIAAPAAAAVCDLRMEPKIPE PFVWPNGDARPASAAELDMPVVDVGVLRDGDAEGLRRAAAQVAAACATHGFFQVSEHG VDAALARAALDGASDFFRLPLAEKRRARRVPGTVSGYTSAHADRFASKLPWKETLSFG FHDRAAAPVVADYFSSTLGPDFAPMGRVYQKYCEEMKELSLTIMELLELSLGVERGYY REFFADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDVGGLEVLVDGEWRP VSPVPGAMVINIGDTFMALSNGRYKSCLHRAVVNQRRERRSLAFFLCPREDRVVRPPP SAATPQHYPDFTWADLMRFTQRHYRADTRTLDAFTRWLAPPAADAAATAQVEAAS</aaseq>|<br />
DNA = <dnaseqindica>1..557#660..981#2453..2743#atggtggccgagcaccccacgccaccacagccgcaccaaccaccgcccatggactccaccgccggctctggcattgccgccccggcggcggcggcggtgtgcgacctgaggatggagcccaagatcccggagccattcgtgtggccgaacggcgacgcgaggccggcgtcggcggcggagctggacatgcccgtggtcgacgtgggcgtgctccgcgacggcgacgccgaggggctgcgccgcgccgcggcgcaggtggccgccgcgtgcgccacgcacgggttcttccaggtgtccgagcacggcgtcgacgccgctctggcgcgcgccgcgctcgacggcgccagcgacttcttccgcctcccgctcgccgagaagcgccgcgcgcgccgcgtcccgggcaccgtgtccggctacaccagcgcccacgccgaccgcttcgcctccaagctcccatggaaggagaccctctccttcggcttccacgaccgcgccgccgcccccgtcgtcgccgactacttctccagcaccctcggccccgacttcgcgccaatggggtaattaaaacgatggtggacgacattgcatttcaaattcaaaacaaattcaaaacacaccgaccgagattatgctgaattcaaacgcgtttgtgcgcgcaggagggtgtaccagaagtactgcgaggagatgaaggagctgtcgctgacgatcatggaactcctggagctgagcctgggcgtggagcgaggctactacagggagttcttcgcggacagcagctcaatcatgcggtgcaactactacccgccatgcccggagccggagcggacgctcggcacgggcccgcactgcgaccccaccgccctcaccatcctcctccaggacgacgtcggcggcctcgaggtcctcgtcgacggcgaatggcgccccgtcagccccgtccccggcgccatggtcatcaacatcggcgacaccttcatggtaaaccatctcctattctcctctcctctgttctcctctgcttcgaagcaacagaacaagtaattcaagcttttttttctctctcgcgcgaaattgacgagaaaaataagatcgtggtaggggcggggctttcagctgaaagcgggaagaaaccgacctgacgtgatttctctgttccaatcacaaacaatggaatgccccactcctccatgtgttatgatttatctcacatcttatagttaataggagtaagtaacaagctacttttttcatattatagttcgtttgattttttttttttaaagtttttttagttttatccaaatttattgaaaaacttagcaacgtttataataccaaattagtctcatttagtttaatattgtatatattttgataatatatttatgttatattaaaaatattactatatttttctataaacattattaaaagccatttataatataaaatggaaggagtaattaatatggatctcccccgacatgagaatattttccgatggtgtgacgacgccatgtaagcttcggtgggcctggacggccagaggtgccaacagccacgtccaacaacccctgggtccccccctaacactccaaacagtagtgagtagtgtctcgtcgcgttttagtatttgatgacaaacaaagtgtgagttgagttagccaccaccaacttgcacacgagcacatacatttgtgtccattctcgccagtcacttccatctctagtcctaactcctatctagcgatgtaagcggataatttcatcatccgtatataaacctgtttgttatagttaatttcctatataatactataacagtatacattttaaaagaaaacaaaattaggataaacaggccctgctcctatccatccatggcacttggaaggaccagactcggtcatgccatgccaagccaagatatgggttatggaagagtagagaagaggagagatgagagataagcatgcgttctcctcctcgttggatgtgtattttggagggatttgtgtagtagtagcagcggcgccgcggggacggatgcggatggtggcgctttcggtggcgttttcccgggggggttttggtttggcgcttgggggggatggcatggcgcggcgtgcggctgcacgccacacacacgcgcgcgcacgcacgtacgtcgtcgtcgccgcgggcggacggtagcttagggtggtgtgttccgcgcgcgggcgcggattgttccatgccgatcgatttggcgccaccctcgccgcggctcttgtcgcgtcgtgcgcctctctcgcgcggtttgtccttgtcgcgttgctcagccggcgacgggggcacggacattggcgatgtagccctgcacgtgtcggcctctccgttgatgaatgatgatgtatgtatgtatttttttttgtctgaaggaatttgtggggaattgttgtgtgtgcaggcgctgtcgaacgggaggtataagagctgcctgcacagggcggtggtgaaccagcggcgggagcggcggtcgctggcgttcttcctgtgcccgcgggaggacagggtggtgcggccgccgccgagcgccgccacgccgcagcactacccggacttcacctgggccgacctcatgcgcttcacgcagcgccactaccgcgccgacacccgcacgctcgacgccttcacgcgctggctcgcgccgccggccgccgacgccgccgcgacggcgcaggtcgaggcggccagctga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051549.1 RefSeq:Os01g0883800]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800&diff=69917Os01g08838002012-07-12T22:52:26Z<p>Daweih: </p>
<hr />
<div>The rice ''semidwarf-1 (sd1)'' is well known as the "green revolution gene" and controls the plant height of rice.<br />
<br />
== Annotated Information ==<br />
=== Function ===<br />
This gene is originally derived from the chinese cultivar Dee-Geo-Woo-Gen(DGWG). It encodes an oxidase enzyme involved in the biosynthesis of gibberellin, which is a plant growth hormone. The rice genome carries at least two GA20ox genes (GA20ox-1 and GA20ox-2).SD1 corresponds to GA20ox-2. Mutation of SD1 will cause a semi-dwarf phenotype of rice without seed yield being affected <ref name="ref1" />. It is not surprising that a rice semidwarfing gene encodes GA20-ox since successful production of semidwarf plants using antisense or overexpressed GA20-ox genes has been reported in Arabidopsis, Solanum dulcamara, potato, and lettuce.<br />
<br />
It is a key enzyme in the biosynthesis of gibberellin that catalyses the three steps GA53->GA44->GA19->GA20. Impaired GA 20-oxidase activity will cause elevated content of GA53, and reduced amount of G20 <ref name="ref1" />.<ref name="ref2" />. However, there is slight difference between different strains. The extent of GA1 is lower in Doongara (semi-dwarf rice strain) when compared with Kyeema (tall), while there is no significant difference between Calrose76 (semi-dwarf rice strain) and Calrose (tall). Calrose76 has lower contents of GA44 and of GA19 than Calrose, while there is no significant difference between Doongara (semi-dwarf rice strain) when compared with Kyeema (tall) <ref name="ref2" />.<br />
<br />
GO asignment(s): GO:0005506, GO:0016216, GO:0017000<br />
<br />
=== Mutation ===<br />
‘dee-geo-woo-gen’ (semi-dwarf rice strain) and IR8 (semi-dwarf rice strain): A 383-base-pair deletion, which induces a frameshift that creates a stop codon in SD1 may be related with the semi-dwarf phenotype <ref name="ref1" />. <br />
<br />
Doongara (semi-dwarf rice strain): A 280-bp deletion within the coding region, which spans parts of exon 1 and exon 2, is predicted to result in a truncated, nonfunctional polypeptide containing the first 99 amino acid residues of the GA 2-oxidase, followed by an additional 3 amino acids that are in the wrong reading frame before a termination codon <ref name="ref2" />.<br />
<br />
Calrose76 (semi-dwarf rice strain): The DNA sequence of Calrose76 is identical to Calrose (tall) except for a C to T transition at position 798 that resulted in a change of the predicted amino acid leucine (Leu-266) in Calrose to phenylalanine in Calrose76 <ref name="ref2" />.<br />
<br />
<br />
<br />
=== Expression ===<br />
This gene is strongly expressed in the leaf blade, stem and unopened flower, whereas GA20ox-1 is predominantly expressed in the unopened flower <ref name="ref1" />.<br />
<br />
Primers for DNA amplication: forward primer, 5'-CAACTCACTCCCGCTCAACACAGC-3', and reverse primer, 5'-TTTGAAATGCAATGTCGTCCACC-3' were used to amplify exon 1; forward primer, 5'-GCGCCAATGGGGTAATTAAAACG-3' and reverse primer, 5'-GGCATTCCATTGTTTGTGATTGG-3' were used for exon 2; forward primer, 5'-GTTTGTCCTTGTCGCGTTGCTCAG-3' and reverse primer, 5'-TCTGTTCGTTCCGTTTCGTTCCG-3' were used for exon 3 <ref name="ref2" />.<br />
<br />
Primers for RT-PCR: forward primer 5'-CAACTCACTCCCGCTCAACACAGC- 3' located 22-bp upstream from the translation start, and a reverse primer 5'-GTTCGTTCCGTTTCGGTTCCG- 3' eight-bp downstream from the TGA stop codon <ref name="ref2" />.<br />
<br />
=== Evolution ===<br />
This gene is conserved in ''Arabidopsis'' (47% identity) and pea(50% identity). GA20ox-2 shows 47.8% identity to<br />
GA20ox-1 in rice. There are at least three GA20-ox genes in Arabidopsis <ref name="ref1" />.<ref name="ref2" />.<br />
<br />
== Labs working on this gene ==<br />
Bioscience Center, and Graduate School of Bioagricultural Science, Nagoya University, Nagoya 464-8601, Japan<br />
Honda R&D, Wako Research Center, Wako 351-0193, Japan<br />
<br />
International Rice Research Institute, Manila, DAPO Box 7777, Philippines<br />
<br />
BioResources Center, and Plant Molecular Biology Laboratory, Riken, Tsukuba 305-0074, Japan<br />
<br />
Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra ACT 2601, Australia<br />
<br />
Plant Genome Center, 1-25-2 Kannondai, Tsukuba, Ibaraki 305-0856, Japan<br />
<br />
== References ==<br />
WHERE CITE<ref name="ref3" />?<br />
<references><br />
<ref name="ref1">Sasaki A, Ashikari M, Ueguchi-Tanaka M, Itoh H, Nishimura A, et al. (2002) Green revolution: a mutant gibberellin-synthesis gene in rice. Nature 416: 701-702.</ref><br />
<ref name="ref2">Spielmeyer W, Ellis MH, Chandler PM (2002) Semidwarf (sd-1), "green revolution" rice, contains a defective gibberellin 20-oxidase gene. Proc Natl Acad Sci U S A 99: 9043-9048.</ref><br />
<ref name="ref3">Monna L, Kitazawa N, Yoshino R, Suzuki J, Masuda H, et al. (2002) Positional cloning of rice semidwarfing gene, sd-1: rice "green revolution gene" encodes a mutant enzyme involved in gibberellin synthesis. DNA Res 9: 11-17.</ref><br />
</references><br />
<br />
<br />
== Structured Information ==<br />
{{JaponicaGene|<br />
GeneName = Os01g0883800|<br />
Description = Gibberellin 20 oxidase 2 (EC 1.14.11.-) (Gibberellin C-20 oxidase 2) (GA 20-oxidase 2) (Os20ox2) (Semidwarf-1 protein)|<br />
Version = NM_001051549.1 GI:115441468 GeneID:4325003|<br />
Length = 2743 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0883800, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:40138313..40141055|<br />
CDS = 40138313..40138869,40138972..40139293,40140765..40141055|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:40138313..40141055<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:40138313..40141055<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggtggccgagcaccccacgccaccacagccgcaccaaccaccgcccatggactccaccgccggctctggcattgccgccccggcggcggcggcggtgtgcgacctgaggatggagcccaagatcccggagccattcgtgtggccgaacggcgacgcgaggccggcgtcggcggcggagctggacatgcccgtggtcgacgtgggcgtgctccgcgacggcgacgccgaggggctgcgccgcgccgcggcgcaggtggccgccgcgtgcgccacgcacgggttcttccaggtgtccgagcacggcgtcgacgccgctctggcgcgcgccgcgctcgacggcgccagcgacttcttccgcctcccgctcgccgagaagcgccgcgcgcgccgcgtcccgggcaccgtgtccggctacaccagcgcccacgccgaccgcttcgcctccaagctcccatggaaggagaccctctccttcggcttccacgaccgcgccgccgcccccgtcgtcgccgactacttctccagcaccctcggccccgacttcgcgccaatggggagggtgtaccagaagtactgcgaggagatgaaggagctgtcgctgacgatcatggaactcctggagctgagcctgggcgtggagcgaggctactacagggagttcttcgcggacagcagctcaatcatgcggtgcaactactacccgccatgcccggagccggagcggacgctcggcacgggcccgcactgcgaccccaccgccctcaccatcctcctccaggacgacgtcggcggcctcgaggtcctcgtcgacggcgaatggcgccccgtcagccccgtccccggcgccatggtcatcaacatcggcgacaccttcatggcgctgtcgaacgggaggtataagagctgcctgcacagggcggtggtgaaccagcggcgggagcggcggtcgctggcgttcttcctgtgcccgcgggaggacagggtggtgcggccgccgccgagcgccgccacgccgcagcactacccggacttcacctgggccgacctcatgcgcttcacgcagcgccactaccgcgccgacacccgcacgctcgacgccttcacgcgctggctcgcgccgccggccgccgacgccgccgcgacggcgcaggtcgaggcggccagctga</cdnaseq>|<br />
AA = <aaseq>MVAEHPTPPQPHQPPPMDSTAGSGIAAPAAAAVCDLRMEPKIPE PFVWPNGDARPASAAELDMPVVDVGVLRDGDAEGLRRAAAQVAAACATHGFFQVSEHG VDAALARAALDGASDFFRLPLAEKRRARRVPGTVSGYTSAHADRFASKLPWKETLSFG FHDRAAAPVVADYFSSTLGPDFAPMGRVYQKYCEEMKELSLTIMELLELSLGVERGYY REFFADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDVGGLEVLVDGEWRP VSPVPGAMVINIGDTFMALSNGRYKSCLHRAVVNQRRERRSLAFFLCPREDRVVRPPP SAATPQHYPDFTWADLMRFTQRHYRADTRTLDAFTRWLAPPAADAAATAQVEAAS</aaseq>|<br />
DNA = <dnaseqindica>1..557#660..981#2453..2743#atggtggccgagcaccccacgccaccacagccgcaccaaccaccgcccatggactccaccgccggctctggcattgccgccccggcggcggcggcggtgtgcgacctgaggatggagcccaagatcccggagccattcgtgtggccgaacggcgacgcgaggccggcgtcggcggcggagctggacatgcccgtggtcgacgtgggcgtgctccgcgacggcgacgccgaggggctgcgccgcgccgcggcgcaggtggccgccgcgtgcgccacgcacgggttcttccaggtgtccgagcacggcgtcgacgccgctctggcgcgcgccgcgctcgacggcgccagcgacttcttccgcctcccgctcgccgagaagcgccgcgcgcgccgcgtcccgggcaccgtgtccggctacaccagcgcccacgccgaccgcttcgcctccaagctcccatggaaggagaccctctccttcggcttccacgaccgcgccgccgcccccgtcgtcgccgactacttctccagcaccctcggccccgacttcgcgccaatggggtaattaaaacgatggtggacgacattgcatttcaaattcaaaacaaattcaaaacacaccgaccgagattatgctgaattcaaacgcgtttgtgcgcgcaggagggtgtaccagaagtactgcgaggagatgaaggagctgtcgctgacgatcatggaactcctggagctgagcctgggcgtggagcgaggctactacagggagttcttcgcggacagcagctcaatcatgcggtgcaactactacccgccatgcccggagccggagcggacgctcggcacgggcccgcactgcgaccccaccgccctcaccatcctcctccaggacgacgtcggcggcctcgaggtcctcgtcgacggcgaatggcgccccgtcagccccgtccccggcgccatggtcatcaacatcggcgacaccttcatggtaaaccatctcctattctcctctcctctgttctcctctgcttcgaagcaacagaacaagtaattcaagcttttttttctctctcgcgcgaaattgacgagaaaaataagatcgtggtaggggcggggctttcagctgaaagcgggaagaaaccgacctgacgtgatttctctgttccaatcacaaacaatggaatgccccactcctccatgtgttatgatttatctcacatcttatagttaataggagtaagtaacaagctacttttttcatattatagttcgtttgattttttttttttaaagtttttttagttttatccaaatttattgaaaaacttagcaacgtttataataccaaattagtctcatttagtttaatattgtatatattttgataatatatttatgttatattaaaaatattactatatttttctataaacattattaaaagccatttataatataaaatggaaggagtaattaatatggatctcccccgacatgagaatattttccgatggtgtgacgacgccatgtaagcttcggtgggcctggacggccagaggtgccaacagccacgtccaacaacccctgggtccccccctaacactccaaacagtagtgagtagtgtctcgtcgcgttttagtatttgatgacaaacaaagtgtgagttgagttagccaccaccaacttgcacacgagcacatacatttgtgtccattctcgccagtcacttccatctctagtcctaactcctatctagcgatgtaagcggataatttcatcatccgtatataaacctgtttgttatagttaatttcctatataatactataacagtatacattttaaaagaaaacaaaattaggataaacaggccctgctcctatccatccatggcacttggaaggaccagactcggtcatgccatgccaagccaagatatgggttatggaagagtagagaagaggagagatgagagataagcatgcgttctcctcctcgttggatgtgtattttggagggatttgtgtagtagtagcagcggcgccgcggggacggatgcggatggtggcgctttcggtggcgttttcccgggggggttttggtttggcgcttgggggggatggcatggcgcggcgtgcggctgcacgccacacacacgcgcgcgcacgcacgtacgtcgtcgtcgccgcgggcggacggtagcttagggtggtgtgttccgcgcgcgggcgcggattgttccatgccgatcgatttggcgccaccctcgccgcggctcttgtcgcgtcgtgcgcctctctcgcgcggtttgtccttgtcgcgttgctcagccggcgacgggggcacggacattggcgatgtagccctgcacgtgtcggcctctccgttgatgaatgatgatgtatgtatgtatttttttttgtctgaaggaatttgtggggaattgttgtgtgtgcaggcgctgtcgaacgggaggtataagagctgcctgcacagggcggtggtgaaccagcggcgggagcggcggtcgctggcgttcttcctgtgcccgcgggaggacagggtggtgcggccgccgccgagcgccgccacgccgcagcactacccggacttcacctgggccgacctcatgcgcttcacgcagcgccactaccgcgccgacacccgcacgctcgacgccttcacgcgctggctcgcgccgccggccgccgacgccgccgcgacggcgcaggtcgaggcggccagctga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051549.1 RefSeq:Os01g0883800]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Daweihhttps://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0883800&diff=69844Os01g08838002012-07-12T22:03:25Z<p>Daweih: </p>
<hr />
<div>The rice ''semidwarf-1 (sd1)'' is well known as the "green revolution gene" and controls the plant height of rice.<br />
<br />
== Annotated Information ==<br />
=== Function ===<br />
This gene is originally derived from the chinese cultivar Dee-Geo-Woo-Gen(DGWG). It encodes an oxidase enzyme involved in the biosynthesis of gibberellin, which is a plant growth hormone. The rice genome carries at least two GA20ox genes (GA20ox-1 and GA20ox-2).SD1 corresponds to GA20ox-2. Mutation of SD1 will cause a semi-dwarf phenotype of rice without seed yield being affected <ref>Sasaki A, Ashikari M, Ueguchi-Tanaka M, Itoh H, Nishimura A, et al. (2002) Green revolution: a mutant gibberellin-synthesis gene in rice. Nature 416: 701-702.</ref>. It is not surprising that a rice semidwarfing gene encodes GA20-ox since successful production of semidwarf plants using antisense or overexpressed GA20-ox genes has been reported in Arabidopsis, Solanum dulcamara, potato, and lettuce.<br />
<br />
It is a key enzyme in the biosynthesis of gibberellin that catalyses the three steps GA53->GA44->GA19->GA20. Impaired GA 20-oxidase activity will cause elevated content of GA53, and reduced amount of G20 [1][2]. However, there is slight difference between different strains. The extent of GA1 is lower in Doongara (semi-dwarf rice strain) when compared with Kyeema (tall), while there is no significant difference between Calrose76 (semi-dwarf rice strain) and Calrose (tall). Calrose76 has lower contents of GA44 and of GA19 than Calrose, while there is no significant difference between Doongara (semi-dwarf rice strain) when compared with Kyeema (tall) [2].<br />
<br />
GO asignment(s): GO:0005506, GO:0016216, GO:0017000<br />
<br />
=== Mutation ===<br />
‘dee-geo-woo-gen’ (semi-dwarf rice strain) and IR8 (semi-dwarf rice strain): A 383-base-pair deletion, which induces a frameshift that creates a stop codon in SD1 may be related with the semi-dwarf phenotype [1]. <br />
<br />
Doongara (semi-dwarf rice strain): A 280-bp deletion within the coding region, which spans parts of exon 1 and exon 2, is predicted to result in a truncated, nonfunctional polypeptide containing the first 99 amino acid residues of the GA 2-oxidase, followed by an additional 3 amino acids that are in the wrong reading frame before a termination codon [2].<br />
<br />
Calrose76 (semi-dwarf rice strain): The DNA sequence of Calrose76 is identical to Calrose (tall) except for a C to T transition at position 798 that resulted in a change of the predicted amino acid leucine (Leu-266) in Calrose to phenylalanine in Calrose76 [2].<br />
<br />
<br />
<br />
=== Expression ===<br />
This gene is strongly expressed in the leaf blade, stem and unopened flower, whereas GA20ox-1 is predominantly expressed in the unopened flower [1].<br />
<br />
Primers for DNA amplication: forward primer, 5'-CAACTCACTCCCGCTCAACACAGC-3', and reverse primer, 5'-TTTGAAATGCAATGTCGTCCACC-3' were used to amplify exon 1; forward primer, 5'-GCGCCAATGGGGTAATTAAAACG-3' and reverse primer, 5'-GGCATTCCATTGTTTGTGATTGG-3' were used for exon 2; forward primer, 5'-GTTTGTCCTTGTCGCGTTGCTCAG-3' and reverse primer, 5'-TCTGTTCGTTCCGTTTCGTTCCG-3' were used for exon 3 [2].<br />
<br />
Primers for RT-PCR: forward primer 5'-CAACTCACTCCCGCTCAACACAGC- 3' located 22-bp upstream from the translation start, and a reverse primer 5'-GTTCGTTCCGTTTCGGTTCCG- 3' eight-bp downstream from the TGA stop codon [2].<br />
<br />
=== Evolution ===<br />
This gene is conserved in ''Arabidopsis'' (47% identity) and pea(50% identity). GA20ox-2 shows 47.8% identity to<br />
GA20ox-1 in rice. There are at least three GA20-ox genes in Arabidopsis [1][2].<br />
<br />
== Labs working on this gene ==<br />
Bioscience Center, and Graduate School of Bioagricultural Science, Nagoya University, Nagoya 464-8601, Japan<br />
Honda R&D, Wako Research Center, Wako 351-0193, Japan<br />
<br />
International Rice Research Institute, Manila, DAPO Box 7777, Philippines<br />
<br />
BioResources Center, and Plant Molecular Biology Laboratory, Riken, Tsukuba 305-0074, Japan<br />
<br />
Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra ACT 2601, Australia<br />
<br />
Plant Genome Center, 1-25-2 Kannondai, Tsukuba, Ibaraki 305-0856, Japan<br />
<br />
== References ==<br />
<references /><br />
1. <br />
<br />
2. Spielmeyer W, Ellis MH, Chandler PM (2002) Semidwarf (sd-1), "green revolution" rice, contains a defective gibberellin 20-oxidase gene. Proc Natl Acad Sci U S A 99: 9043-9048.<br />
<br />
3. Monna L, Kitazawa N, Yoshino R, Suzuki J, Masuda H, et al. (2002) Positional cloning of rice semidwarfing gene, sd-1: rice "green revolution gene" encodes a mutant enzyme involved in gibberellin synthesis. DNA Res 9: 11-17.<br />
<br />
== Structured Information ==<br />
{{JaponicaGene|<br />
GeneName = Os01g0883800|<br />
Description = Gibberellin 20 oxidase 2 (EC 1.14.11.-) (Gibberellin C-20 oxidase 2) (GA 20-oxidase 2) (Os20ox2) (Semidwarf-1 protein)|<br />
Version = NM_001051549.1 GI:115441468 GeneID:4325003|<br />
Length = 2743 bp|<br />
Definition = Oryza sativa Japonica Group Os01g0883800, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br />
AP = Chromosome 1:40138313..40141055|<br />
CDS = 40138313..40138869,40138972..40139293,40140765..40141055|<br />
GCID = <gbrowseImage1><br />
name=NC_008394:40138313..40141055<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008394:40138313..40141055<br />
source=RiceChromosome01<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggtggccgagcaccccacgccaccacagccgcaccaaccaccgcccatggactccaccgccggctctggcattgccgccccggcggcggcggcggtgtgcgacctgaggatggagcccaagatcccggagccattcgtgtggccgaacggcgacgcgaggccggcgtcggcggcggagctggacatgcccgtggtcgacgtgggcgtgctccgcgacggcgacgccgaggggctgcgccgcgccgcggcgcaggtggccgccgcgtgcgccacgcacgggttcttccaggtgtccgagcacggcgtcgacgccgctctggcgcgcgccgcgctcgacggcgccagcgacttcttccgcctcccgctcgccgagaagcgccgcgcgcgccgcgtcccgggcaccgtgtccggctacaccagcgcccacgccgaccgcttcgcctccaagctcccatggaaggagaccctctccttcggcttccacgaccgcgccgccgcccccgtcgtcgccgactacttctccagcaccctcggccccgacttcgcgccaatggggagggtgtaccagaagtactgcgaggagatgaaggagctgtcgctgacgatcatggaactcctggagctgagcctgggcgtggagcgaggctactacagggagttcttcgcggacagcagctcaatcatgcggtgcaactactacccgccatgcccggagccggagcggacgctcggcacgggcccgcactgcgaccccaccgccctcaccatcctcctccaggacgacgtcggcggcctcgaggtcctcgtcgacggcgaatggcgccccgtcagccccgtccccggcgccatggtcatcaacatcggcgacaccttcatggcgctgtcgaacgggaggtataagagctgcctgcacagggcggtggtgaaccagcggcgggagcggcggtcgctggcgttcttcctgtgcccgcgggaggacagggtggtgcggccgccgccgagcgccgccacgccgcagcactacccggacttcacctgggccgacctcatgcgcttcacgcagcgccactaccgcgccgacacccgcacgctcgacgccttcacgcgctggctcgcgccgccggccgccgacgccgccgcgacggcgcaggtcgaggcggccagctga</cdnaseq>|<br />
AA = <aaseq>MVAEHPTPPQPHQPPPMDSTAGSGIAAPAAAAVCDLRMEPKIPE PFVWPNGDARPASAAELDMPVVDVGVLRDGDAEGLRRAAAQVAAACATHGFFQVSEHG VDAALARAALDGASDFFRLPLAEKRRARRVPGTVSGYTSAHADRFASKLPWKETLSFG FHDRAAAPVVADYFSSTLGPDFAPMGRVYQKYCEEMKELSLTIMELLELSLGVERGYY REFFADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDVGGLEVLVDGEWRP VSPVPGAMVINIGDTFMALSNGRYKSCLHRAVVNQRRERRSLAFFLCPREDRVVRPPP SAATPQHYPDFTWADLMRFTQRHYRADTRTLDAFTRWLAPPAADAAATAQVEAAS</aaseq>|<br />
DNA = <dnaseqindica>1..557#660..981#2453..2743#atggtggccgagcaccccacgccaccacagccgcaccaaccaccgcccatggactccaccgccggctctggcattgccgccccggcggcggcggcggtgtgcgacctgaggatggagcccaagatcccggagccattcgtgtggccgaacggcgacgcgaggccggcgtcggcggcggagctggacatgcccgtggtcgacgtgggcgtgctccgcgacggcgacgccgaggggctgcgccgcgccgcggcgcaggtggccgccgcgtgcgccacgcacgggttcttccaggtgtccgagcacggcgtcgacgccgctctggcgcgcgccgcgctcgacggcgccagcgacttcttccgcctcccgctcgccgagaagcgccgcgcgcgccgcgtcccgggcaccgtgtccggctacaccagcgcccacgccgaccgcttcgcctccaagctcccatggaaggagaccctctccttcggcttccacgaccgcgccgccgcccccgtcgtcgccgactacttctccagcaccctcggccccgacttcgcgccaatggggtaattaaaacgatggtggacgacattgcatttcaaattcaaaacaaattcaaaacacaccgaccgagattatgctgaattcaaacgcgtttgtgcgcgcaggagggtgtaccagaagtactgcgaggagatgaaggagctgtcgctgacgatcatggaactcctggagctgagcctgggcgtggagcgaggctactacagggagttcttcgcggacagcagctcaatcatgcggtgcaactactacccgccatgcccggagccggagcggacgctcggcacgggcccgcactgcgaccccaccgccctcaccatcctcctccaggacgacgtcggcggcctcgaggtcctcgtcgacggcgaatggcgccccgtcagccccgtccccggcgccatggtcatcaacatcggcgacaccttcatggtaaaccatctcctattctcctctcctctgttctcctctgcttcgaagcaacagaacaagtaattcaagcttttttttctctctcgcgcgaaattgacgagaaaaataagatcgtggtaggggcggggctttcagctgaaagcgggaagaaaccgacctgacgtgatttctctgttccaatcacaaacaatggaatgccccactcctccatgtgttatgatttatctcacatcttatagttaataggagtaagtaacaagctacttttttcatattatagttcgtttgattttttttttttaaagtttttttagttttatccaaatttattgaaaaacttagcaacgtttataataccaaattagtctcatttagtttaatattgtatatattttgataatatatttatgttatattaaaaatattactatatttttctataaacattattaaaagccatttataatataaaatggaaggagtaattaatatggatctcccccgacatgagaatattttccgatggtgtgacgacgccatgtaagcttcggtgggcctggacggccagaggtgccaacagccacgtccaacaacccctgggtccccccctaacactccaaacagtagtgagtagtgtctcgtcgcgttttagtatttgatgacaaacaaagtgtgagttgagttagccaccaccaacttgcacacgagcacatacatttgtgtccattctcgccagtcacttccatctctagtcctaactcctatctagcgatgtaagcggataatttcatcatccgtatataaacctgtttgttatagttaatttcctatataatactataacagtatacattttaaaagaaaacaaaattaggataaacaggccctgctcctatccatccatggcacttggaaggaccagactcggtcatgccatgccaagccaagatatgggttatggaagagtagagaagaggagagatgagagataagcatgcgttctcctcctcgttggatgtgtattttggagggatttgtgtagtagtagcagcggcgccgcggggacggatgcggatggtggcgctttcggtggcgttttcccgggggggttttggtttggcgcttgggggggatggcatggcgcggcgtgcggctgcacgccacacacacgcgcgcgcacgcacgtacgtcgtcgtcgccgcgggcggacggtagcttagggtggtgtgttccgcgcgcgggcgcggattgttccatgccgatcgatttggcgccaccctcgccgcggctcttgtcgcgtcgtgcgcctctctcgcgcggtttgtccttgtcgcgttgctcagccggcgacgggggcacggacattggcgatgtagccctgcacgtgtcggcctctccgttgatgaatgatgatgtatgtatgtatttttttttgtctgaaggaatttgtggggaattgttgtgtgtgcaggcgctgtcgaacgggaggtataagagctgcctgcacagggcggtggtgaaccagcggcgggagcggcggtcgctggcgttcttcctgtgcccgcgggaggacagggtggtgcggccgccgccgagcgccgccacgccgcagcactacccggacttcacctgggccgacctcatgcgcttcacgcagcgccactaccgcgccgacacccgcacgctcgacgccttcacgcgctggctcgcgccgccggccgccgacgccgccgcgacggcgcaggtcgaggcggccagctga</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051549.1 RefSeq:Os01g0883800]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 1]]<br />
[[Category:Chromosome 1]]</div>Daweih