RiceWiki - User contributions [en]

Os02g0610500

2014-05-20T03:57:32Z

Duan Yangmiao:

Plants recognize environmental factors to determine ﬂowering time. CONSTANS (CO) plays a central role in the photoperiod ﬂowering pathway of Arabidopsis, and CO protein stability is modulated by photoreceptor.
==Annotated Information==

===function===
[[File:Figure1.jpg|right|thumb|275px|'''Figure 1.''' '' Phenotypes of oscol4-1, oscol4-2, and WT at heading stage .(from reference)<ref name="ref1" />).'']]
[[File:Figure2.jpg|right|thumb|275px|'''Figure 2.''' '' OsCOL4 has two exons (boxes) and one intron (line between boxes). Closed boxes indicate coding region, and open boxes show the 5¢ and 3¢ untranslated regions (UTR).(from reference)<ref name="ref1" />).'']]
[[File:Figure3.jpg|right|thumb|275px|'''Figure 3.''' '' Quantitative RT-PCR analyses of OsCOL4 in WT and OsCOL4-D plants. Samples were harvested at ZT 16 h from second leaf blades from top of plants grown for 45 days under LD. Y axis, transcript level of OsCOL4 relative to that of rice ubi. (from reference)<ref name="ref1" />).'']]
OsCOL4, a member of the CONSTANS-like (COL) family in rice.OsCOL4 is a constitutive repressorfunctioning upstream of Ehd1 and functions independently from previously reported ﬂowering pathways. In osphyB mutants, OsCOL4 expression was decreased and osphyB oscol4 double mutants ﬂowered at the same time as the osphyB single mutants,indicating OsCOL4 functions downstream of OsphyB.<ref name="ref1" />

===Expression===
OsCOL4 null mutants ﬂowered early under short or long days. In contrast, OsCOL4 activation-tagging mutants (OsCOL4-D) ﬂowered late in either environment. Transcripts of Ehd1, Hd3a, and RFT1 were increased in the oscol4 mutants, but reduced in the OsCOL4-D mutants. By comparison, levels of Hd1, OsID1, OsMADS50, OsMADS51, and OsMADS56 transcripts were not signiﬁcantly changed in oscol4 or OsCOL4-D.In osphyB mutants, OsCOL4 expression was decreased and osphyB oscol4 double mutants ﬂowered at the same time as the osphyB single mutants<ref name="ref1" />

===Evolution===

Plants recognize environmental factors to determine ﬂowering time. CONSTANS (CO) plays a central role in the photoperiod ﬂowering pathway of Arabidopsis, and CO protein stability is modulated by photoreceptor<ref name="ref1" />s. In the former, GIGANTEA (GI) activates CONSTANS (CO), which promotes Flowering Locus T (FT) expression<ref name="ref2" />. The CO gene is a member of a B-box transcription factor family which contains a CCT (CO, CO-like, and TOC1) domain at the C-terminal end<ref name="ref3" /> .In rice, Hd1, an ortholog of CO, acts as a ﬂowering promoter, and phytochromes repress Hd1 expression.The rice genome contains 16 OsCOL genes <ref name="ref4" />. Among them, Hd1 was identiﬁed ﬁrst via QTL analyses <ref name="ref5" />. Two groups of rice COL proteins differ from those in Arabidopsis <ref name="ref4" />.

==Labs working on this gene==
*Department of Life Science, Pohang University of Science and Technology (POSTECH), Pohang 790–784, Korea
*Crop Biotech Institute, Kyung Hee University, Yongin 446–701, Korea
*Department of Plant Systems Biotech, Kyung Hee University, Yongin 446–701, Korea
*Gyeongsang National University, Korea
*the Plant Functional Genomics Laboratory at the POSTECH

==References==
<references>
<ref name="ref1">Yang-Seok Lee; Dong-Hoon Jeong; Dong-Yeon Lee;（2010）OsCOL4 is a constitutive flowering repressor upstream of Ehd1 and downstream of OsphyB The Plant Journal, 63(1): 18-30</ref>
<ref name="ref2">Yanovsky, M.J. and Kay, S.A. (2002) Molecular basis of seasonal time measurement in Arabidopsis. Nature, 419, 308–312</ref>
<ref name="ref3">Robson, F., Costa, M.M.R., Hepworth, S.R.. (2001) Functional importance of conserved domains in the ﬂowering-time gene CONSTANS demonstrated by analysis of mutant alleles and transgenic plants. Plant J. 28, 619631</ref>
<ref name="ref4">Grifﬁths, S., Dunford, R.P., Coupland, G. and Laurie, D.A. (2003) The evolution of CONSTANS-like gene families in barley, rice, and Arabidopsis. Plant Physiol. 131, 1855–1867</ref>
<ref name="ref5">Yano, M., Katayose, Y., Ashikari, M. et al. (2000) Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis ﬂowering time gene CONSTANS. Plant Cell, 12, 24732483</ref>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0610500|
Description = Similar to CONSTANS-like protein CO9 (Fragment)|
Version = NM_001053934.1 GI:115447238 GeneID:4329950|
Length = 1469 bp|
Definition = Oryza sativa Japonica Group Os02g0610500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:24847664..24849132|
CDS = 24847765..24848448,24848530..24848844|
GCID = <gbrowseImage1>
name=NC_008395:24847664..24849132
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:24847664..24849132
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggaggcggtggaggacaaggcgatggtgggagtgggaggagcggtggcggcggggtactcctcgtcgtcgtgggggttggggacgcgggcgtgcgactcgtgcggcggggaggcggcgcggctctactgccgcgcggacggggcgttcctgtgcgcccggtgcgacgcgcgggcgcacggcgccgggtcgcgccacgcgcgggtgtggctgtgcgaggtgtgcgagcacgcgcccgccgccgtcacgtgccgggcggacgccgcggcgctgtgcgccgcctgcgacgccgacatccactcggcgaacccgctcgcgcgcaggcacgagcgcctccccgtcgcgcccttcttcggcccgctcgccgacgcgccgcagcccttccccttctcccaggccgccgcggatgccgccgcggcgcgggaggaggatgcggatgatgaccggagcaacgaggccgaggcggcgtcgtggcttctccccgagcccgacgacaatagccacgaggatagcgccgcagccgccgacgcgttcttcgccgacaccggcgcgtacctcggcgtcgacctggacttcgcccggtccatggacggaatcaaggccatcggggtaccggtcgcgccgcccgagctggacctcaccgccggcagccttttctaccccgaacactccatggcccacagcttgtcgtcgtcggaggtcgcgatcgtaccggacgcgctgtcggcgggctcggcggcgccgcccatggtggtggtggtggcgagcaaggggaaggagagggaggcgcggctgatgcggtacagggagaagcgcaagaaccggcggttcgacaagaccatccggtacgcgtcccgcaaggcgtacgccgagacgcggccgcgcatcaagggccggttcgccaagcgcaccgccgacgccgacgacgacgacgaggcgccatgctcgccggcgttctccgccctcgccgcgtcggacggcgtcgtgccgtcgttctga</cdnaseq>|
AA = <aaseq>MEAVEDKAMVGVGGAVAAGYSSSSWGLGTRACDSCGGEAARLYC RADGAFLCARCDARAHGAGSRHARVWLCEVCEHAPAAVTCRADAAALCAACDADIHSA NPLARRHERLPVAPFFGPLADAPQPFPFSQAAADAAAAREEDADDDRSNEAEAASWLL PEPDDNSHEDSAAAADAFFADTGAYLGVDLDFARSMDGIKAIGVPVAPPELDLTAGSL FYPEHSMAHSLSSSEVAIVPDALSAGSAAPPMVVVVASKGKEREARLMRYREKRKNRR FDKTIRYASRKAYAETRPRIKGRFAKRTADADDDDEAPCSPAFSALAASDGVVPSF</aaseq>|
DNA = <dnaseqindica>102..785#867..1181#gtgcccaacgcccaaaaaacacagccaaactccgcgagaaaccgagctgcgagcgagtgaaacgcaccacgcgccgagcggcaaagcgaagcgaagaagaaatggaggcggtggaggacaaggcgatggtgggagtgggaggagcggtggcggcggggtactcctcgtcgtcgtgggggttggggacgcgggcgtgcgactcgtgcggcggggaggcggcgcggctctactgccgcgcggacggggcgttcctgtgcgcccggtgcgacgcgcgggcgcacggcgccgggtcgcgccacgcgcgggtgtggctgtgcgaggtgtgcgagcacgcgcccgccgccgtcacgtgccgggcggacgccgcggcgctgtgcgccgcctgcgacgccgacatccactcggcgaacccgctcgcgcgcaggcacgagcgcctccccgtcgcgcccttcttcggcccgctcgccgacgcgccgcagcccttccccttctcccaggccgccgcggatgccgccgcggcgcgggaggaggatgcggatgatgaccggagcaacgaggccgaggcggcgtcgtggcttctccccgagcccgacgacaatagccacgaggatagcgccgcagccgccgacgcgttcttcgccgacaccggcgcgtacctcggcgtcgacctggacttcgcccggtccatggacggaatcaaggccatcggggtaccggtcgcgccgcccgagctggacctcaccgccggcagccttttctaccccgaacactccatggcccacagcgtaagcctcactaccacacagctagctctcgtcgatgctcatggcgcggcgttatgacatggtttcctacgcgttccgcagttgtcgtcgtcggaggtcgcgatcgtaccggacgcgctgtcggcgggctcggcggcgccgcccatggtggtggtggtggcgagcaaggggaaggagagggaggcgcggctgatgcggtacagggagaagcgcaagaaccggcggttcgacaagaccatccggtacgcgtcccgcaaggcgtacgccgagacgcggccgcgcatcaagggccggttcgccaagcgcaccgccgacgccgacgacgacgacgaggcgccatgctcgccggcgttctccgccctcgccgcgtcggacggcgtcgtgccgtcgttctgaggaaggacgtacgcacggtacggcgagtcggcgacgtgcgccgtcgtaattttggcgcgccccgtgcgcgcgtgcatgcgtgcgtgtgtgcgacgcatggccccgtgtgacatgaataatatgtacagtagtttttcatccatggacgtagtattctattgtactccttgtactcctactactcctcttctgcctaaccaaggcttgtacattaccatgggagtagctgtttttgcaaccgtgaccatggttcagtgcttcaagttcaagggcgttaatgttactgtc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053934.1 RefSeq:Os02g0610500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0610500

2014-05-14T16:10:08Z

Duan Yangmiao: /* Evolution */

Please input one-sentence summary here.

==Annotated Information==

===function===
OsCOL4, a member of the CONSTANS-like (COL) family in rice.OsCOL4 is a constitutive repressorfunctioning upstream of Ehd1 and functions independently from previously reported ﬂowering pathways. In osphyB mutants, OsCOL4 expression was decreased and osphyB oscol4 double mutants ﬂowered at the same time as the osphyB single mutants,indicating OsCOL4 functions downstream of Os

===Expression===
OsCOL4 nullmutants ﬂowered early under short or long days. In contrast, OsCOL4 activation-tagging mutants (OsCOL4-D) ﬂowered late in either environment. Transcripts of Ehd1, Hd3a, and RFT1 were increased in the oscol4 mutants, but reduced in the OsCOL4-D mutants. By comparison, levels of Hd1, OsID1, OsMADS50, OsMADS51, and OsMADS56 transcripts were not signiﬁcantly changed in oscol4 or OsCOL4-D.In osphyB mutants, OsCOL4 expression was decreased and osphyB oscol4 double mutants ﬂowered at the same time as the osphyB single mutants.

===Evolution===

Plants recognize environmental factors to determine ﬂowering time. CONSTANS (CO) plays a central role in the photoperiod ﬂowering pathway of Arabidopsis, and CO protein stability is modulated by photoreceptors. In the former, GIGANTEA (GI) activates CONSTANS (CO), which promotes Flowering Locus T (FT) expression (Fowler et al.,1999; Park et al., 1999; Samach et al., 2000; Yanovsky and Kay, 2002). The CO gene is a member of a B-box transcription factor family which contains a CCT (CO, CO-like, and TOC1) domain at the C-terminal end (Putterill et al., 1995; Borden, 1998; Robson et al., 2001).In rice, Hd1, an ortholog of CO, acts as a ﬂowering promoter, and phytochromes repress Hd1 expression.The rice genome contains 16 OsCOL genes (Grifﬁths et al.,2003). Among them, Hd1 was identiﬁed ﬁrst via QTL analyses (Yano et al., 2000). Two groups of rice COL proteins differ from those in Arabidopsis (Grifﬁths et al., 2003).

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0610500|
Description = Similar to CONSTANS-like protein CO9 (Fragment)|
Version = NM_001053934.1 GI:115447238 GeneID:4329950|
Length = 1469 bp|
Definition = Oryza sativa Japonica Group Os02g0610500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:24847664..24849132|
CDS = 24847765..24848448,24848530..24848844|
GCID = <gbrowseImage1>
name=NC_008395:24847664..24849132
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:24847664..24849132
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggaggcggtggaggacaaggcgatggtgggagtgggaggagcggtggcggcggggtactcctcgtcgtcgtgggggttggggacgcgggcgtgcgactcgtgcggcggggaggcggcgcggctctactgccgcgcggacggggcgttcctgtgcgcccggtgcgacgcgcgggcgcacggcgccgggtcgcgccacgcgcgggtgtggctgtgcgaggtgtgcgagcacgcgcccgccgccgtcacgtgccgggcggacgccgcggcgctgtgcgccgcctgcgacgccgacatccactcggcgaacccgctcgcgcgcaggcacgagcgcctccccgtcgcgcccttcttcggcccgctcgccgacgcgccgcagcccttccccttctcccaggccgccgcggatgccgccgcggcgcgggaggaggatgcggatgatgaccggagcaacgaggccgaggcggcgtcgtggcttctccccgagcccgacgacaatagccacgaggatagcgccgcagccgccgacgcgttcttcgccgacaccggcgcgtacctcggcgtcgacctggacttcgcccggtccatggacggaatcaaggccatcggggtaccggtcgcgccgcccgagctggacctcaccgccggcagccttttctaccccgaacactccatggcccacagcttgtcgtcgtcggaggtcgcgatcgtaccggacgcgctgtcggcgggctcggcggcgccgcccatggtggtggtggtggcgagcaaggggaaggagagggaggcgcggctgatgcggtacagggagaagcgcaagaaccggcggttcgacaagaccatccggtacgcgtcccgcaaggcgtacgccgagacgcggccgcgcatcaagggccggttcgccaagcgcaccgccgacgccgacgacgacgacgaggcgccatgctcgccggcgttctccgccctcgccgcgtcggacggcgtcgtgccgtcgttctga</cdnaseq>|
AA = <aaseq>MEAVEDKAMVGVGGAVAAGYSSSSWGLGTRACDSCGGEAARLYC RADGAFLCARCDARAHGAGSRHARVWLCEVCEHAPAAVTCRADAAALCAACDADIHSA NPLARRHERLPVAPFFGPLADAPQPFPFSQAAADAAAAREEDADDDRSNEAEAASWLL PEPDDNSHEDSAAAADAFFADTGAYLGVDLDFARSMDGIKAIGVPVAPPELDLTAGSL FYPEHSMAHSLSSSEVAIVPDALSAGSAAPPMVVVVASKGKEREARLMRYREKRKNRR FDKTIRYASRKAYAETRPRIKGRFAKRTADADDDDEAPCSPAFSALAASDGVVPSF</aaseq>|
DNA = <dnaseqindica>102..785#867..1181#gtgcccaacgcccaaaaaacacagccaaactccgcgagaaaccgagctgcgagcgagtgaaacgcaccacgcgccgagcggcaaagcgaagcgaagaagaaatggaggcggtggaggacaaggcgatggtgggagtgggaggagcggtggcggcggggtactcctcgtcgtcgtgggggttggggacgcgggcgtgcgactcgtgcggcggggaggcggcgcggctctactgccgcgcggacggggcgttcctgtgcgcccggtgcgacgcgcgggcgcacggcgccgggtcgcgccacgcgcgggtgtggctgtgcgaggtgtgcgagcacgcgcccgccgccgtcacgtgccgggcggacgccgcggcgctgtgcgccgcctgcgacgccgacatccactcggcgaacccgctcgcgcgcaggcacgagcgcctccccgtcgcgcccttcttcggcccgctcgccgacgcgccgcagcccttccccttctcccaggccgccgcggatgccgccgcggcgcgggaggaggatgcggatgatgaccggagcaacgaggccgaggcggcgtcgtggcttctccccgagcccgacgacaatagccacgaggatagcgccgcagccgccgacgcgttcttcgccgacaccggcgcgtacctcggcgtcgacctggacttcgcccggtccatggacggaatcaaggccatcggggtaccggtcgcgccgcccgagctggacctcaccgccggcagccttttctaccccgaacactccatggcccacagcgtaagcctcactaccacacagctagctctcgtcgatgctcatggcgcggcgttatgacatggtttcctacgcgttccgcagttgtcgtcgtcggaggtcgcgatcgtaccggacgcgctgtcggcgggctcggcggcgccgcccatggtggtggtggtggcgagcaaggggaaggagagggaggcgcggctgatgcggtacagggagaagcgcaagaaccggcggttcgacaagaccatccggtacgcgtcccgcaaggcgtacgccgagacgcggccgcgcatcaagggccggttcgccaagcgcaccgccgacgccgacgacgacgacgaggcgccatgctcgccggcgttctccgccctcgccgcgtcggacggcgtcgtgccgtcgttctgaggaaggacgtacgcacggtacggcgagtcggcgacgtgcgccgtcgtaattttggcgcgccccgtgcgcgcgtgcatgcgtgcgtgtgtgcgacgcatggccccgtgtgacatgaataatatgtacagtagtttttcatccatggacgtagtattctattgtactccttgtactcctactactcctcttctgcctaaccaaggcttgtacattaccatgggagtagctgtttttgcaaccgtgaccatggttcagtgcttcaagttcaagggcgttaatgttactgtc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053934.1 RefSeq:Os02g0610500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]