RiceWiki - User contributions [en]

File:Fig.3 OsYSL2-3.png

2014-05-19T16:24:06Z

Eric Fu:

Os02g0649900

2014-05-19T16:23:50Z

Eric Fu: /* Expression */

OsYSL2 was reported by researchers from Japan as a rice metal-NA transporter that is responsible for the phloem transport of iron and manganese, including the translocation of iron and manganese into the grain.
==Annotated Information==
===Function===
*OsYSL2 is an Fe-regulated metal-NA transporter that is involved in phloem transport and the translocation of mineral nutrients in grains<ref name="ref1" />. The altered expression of OsYSL2changes the localization of Fe, and that OsYSL2 is a critical Fe-nicotianamine transporter important for Fe translocation,especially in the shoots and endosperm <ref name="ref2" />.

*Based on the nucleotide sequence,OsYSL2 was predicted to encode a polypeptide of 674 amino acids containing 14 putative transmembrane domains<ref name="ref1" />. Cells expressing the OsYSL2–GFP fusion protein showed a signal in the plasma membrane, with the presence of the putative transmembrane domains shows that OsYSL2 is a transporter that is localized in the plasma membrane<ref name="ref1" />.
[[File:Fig.1 OsYSL2.gif|right|thumb|250px|'''Figure 1.''' ''Expression and seed iron (Fe) concentrations of OsYSL2i and phenotype of OsYSL2i at the early growth stage.(From reference <ref name="ref2" />).'']]

===Mutant===
*Yasuhiro Ishimaru et al. produced OsYSL2 knock-down lines using the RNAi method. Seven independent lines were generated, and the expression of endogenous OsYSL2 in T1OsYSL2i leaves of lines 3 and 5 was suppressed, as revealed by northern blot analysis(Figure 1a). Fe concentrations in the seeds were also decreased in lines 3 and 5,consistent with the suppression of OsYSL2(Figure 1b).At the early growth stage, the growth of line 5 was impaired compared with the WT, when grown under Fe-sufficient conditions. These growth defects appeared to be more significant when grown under Fe-deficient conditions (Figure 1c–e)<ref name="ref2" />.

*Yasuhiro Ishimaru et al. assessed whether the over expression of OsYSL2(OXOsYSL2) in rice plants could enhance Fe accumulation in the seeds. Constitutive over expression of OsYSL2 decreased the Fe concentration in the brown rice and shoots ,although the Fe concentration was higher in the roots of OXOsYSL2 plants. On the other hand, the Mn concentration was increased in the brown rice and decreased in the roots of OXOsYSL2 plants<ref name="ref2" />.

[[File:Fig.2 OsYSL2-1.png|right|thumb|250px|'''Figure 2.''' ''RT-PCR analysis of OsYSL2 expression in flowers and developing seeds.(From reference <ref name="ref1" />).'']]

===Expression===
*OsYSL2 was of particular interest because no detectable transcripts were present in the roots of either Fe-sufficient or Fe-deficient plants, but dramatic expression was induced in the leaves of Fe-deficient plants<ref name="ref1" />.Research by Yasuhiro Ishimaru et al. showed that the high expression of OsYSL2 in the roots under Fe deficiency was not consistent with previous study<ref name="ref2" />.
*The cell-type specificity of OsYSL2 expression was investigated using promoter (1.5 kb):b-glucuronidase (GUS) analysis.GUS staining was observed in phloem cells of the vascular bundles of leaves and leaf sheaths of Fe-sufficient rice. The phloem-specific expression of the OsYSL2 promoter suggested that OsYSL2 is involved in the phloem transport of Fe <ref name="ref1" />. OsYSL2 is expressed during reproductive development in rice. RT-PCR analysis confirmed that OsYSL2 transcripts were increased during early seed development(figure 2)<ref name="ref1" />.
*In fully mature seeds,strong OsYSL2 expression was observed in the epithelium, the vascular bundle of the scutellum, and the leaf primordium . Two days after sowing, the OsYSL2 expression level increased in the scutellum near the endosperm. Three days after sowing,OsYSL2 expression was observed in the bran and coleoptile<ref name="ref3" />.

[[File:Fig.3 OsYSL2-3.png|right|thumb|250px|'''Figure 3.''' ''The unrooted phylogenic tree for the 18 OsYSL amino acid sequences.(From reference <ref name="ref1" />).'']]

===Evolution===
YS1-like genes in rice Our search for YS1 homologus in the Oryza sativaL. Ssp. japonica (cv. Nipponbare) rice genomic database identified
18 putative OsYSL that exhibited 36–76% sequence similarity to YS1(figure 3) <ref name="ref1" />.

==Labs working on this gene==
*Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
*Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Kawaguchi 332-0012, Japan
*National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan

==References==
<references>
<ref name="ref1"> Koike S, Inoue H, Mizuno D, et al. OsYSL2 is a rice metal‐nicotianamine transporter that is regulated by iron and expressed in the phloem[J]. The Plant Journal, 2004, 39(3): 415-424.PMID 15255870</ref>
<ref name="ref2"> Ishimaru Y, Masuda H, Bashir K, et al. Rice metal‐nicotianamine transporter, OsYSL2, is required for the long‐distance transport of iron and manganese[J]. The Plant Journal, 2010, 62(3): 379-390.PMID 20128878</ref>
<ref name="ref3"> Nozoye T, Inoue H, Takahashi M, et al. The expression of iron homeostasis-related genes during rice germination[J]. Plant molecular biology, 2007, 64(1-2): 35-47.PMID 17333504</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0649900|
Description = Similar to Iron-phytosiderophore transporter protein yellow stripe 1|
Version = NM_001054121.2 GI:297599682 GeneID:4330161|
Length = 3302 bp|
Definition = Oryza sativa Japonica Group Os02g0649900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:27058478..27061779|
CDS = 27058478..27059406,27059491..27059605,27059697..27059886,27059990..27060124,27060204..27060301 ,27061115..27061287,27061395..27061779|
GCID = <gbrowseImage1>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</cdnaseq>|
AA = <aaseq>MEAAAPEIERCDAGDVESDHDGAAAAAERVPPWREQVTARGMVA ALLIGFVYTVIIMKLALTTGIIPTLNVSAALLAFLALRGWTRAPALLLPGGGAASSSS RRRPFTRQENTVVQTCAVACYTMGFGGGFGSSLLALNRKTYELAGVSTPGNSPGSYKE PGVGWMTGFLFAISFVGLLNLLPLRKALIIDYKLTYPSGTATAVLINGFHTPQGENSA KKQVRGFLNCFGISLLWSFFQWFYTGGESCGFLQFPTFGLKAWKQTFYFDFSLTYVGA GMICSHLVNLSALFGAILSWGIMWPLISIQKGKWYPGNVPESSMTSLFGYKSFMCVAL IMGDGLYHFIKVTGITAKSLHERSNRRHAKKATDEDTFVIADMQRDEFFNKDYIPNWL AYAGYALLSIVAVIAIPIMFQQVKWYYVVVAFVLAPVLGFSNAYGTGLTDMNMSYNYG KIALFIFAAWGGRDNGVIAGLVGCGIVKQLVQVSADLMHDFKTGHLTLTSPRSMLVGQ AIGTAMGCIIAPLTFLLFYKAFDIGNPDGYWKAPYALIFRNMAILGVEGFSALPKHCL ELSAGFFAFSVLINLMRDFLPRKYRDYVPLPTAMAVPFLVGANFAIDMCVGSLIVFAW HKINSKESALLVPAVASGFICGDGIWMFPSSLLSLAKVKPPICMKFTPGS</aaseq>|
DNA = <dnaseqindica>2374..3302#2175..2289#1894..2083#1656..1790#1479..1576#493..665#1..385#atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgcgcgccccgctactacttcctgtttgatttctctccccaagtctttcttaatttgttcttgcgatggcgcctgtatataaaattgggggatatcgtgcgcgcaggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggtgtgtactttgcccacaatttttgttccgttggtactgaatatgcaatggattaaattagacctgtccctgcaaattcaggactctctggagttcctgctattatgttcgttaattgttgtgatttgctatctttcagtgaactgtttttttctctcagttttgtttcggatttttggagaatatagctcagttgttgcatgtgaaatctgtgctatattctcagttttgtgctgatattaaaaattgtgaactcactcctattttaagatgatattttacataaacaatctgttgttacatttgtaaatttgtctgatcccagtatacggataggtcatgttttcaagaaagactaggaagtttaagttacccatcagtctaccactatctgttttactttgttaggttcttgatctgctttatcttgctaacctttaaggctttaactagaactacttgttcagactccagagtaatgcacatcgtgtgcatggcatggaaacccaactaacctgcaacagttcaacaccaattgggagttctacatagtacacatcctaatatttggtatgcggcattacatttccattatccttaaccaacaagttgactaaagtgctaaaacgaaatgaacttttttgagacaaataaaatgtcatagactaaatgagacaactgtacacacgaattccctctagatactcttcttcaagtctaaaaaatttgtcattacaatctgtagtttgaagacagaaaccagttttctttgcatttgtctgtcccacctaatgatgtgcatgacgttttcaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagtatggtccaggtctcacttgtgctaaatacatcatcgcagttcgtgatatgaattattaattctacttttcatgccaggaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgtatgcattattggtcacccattgtttgtacccctactttatgcataattgcatattcgtcaggcagccagattctcctaactaccaattttttttgctgcaggttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaaggtcaggcatacaatttacctttttctcaaaatagtctgatgtgaatccataattttatatggtacagtatctctaattttctccttctcagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaaggtaaacgtgctctgcgaaattaactatttccacgttttcttccctcttatatcaccaaggtagctgaagctgagtgaattgcagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054121.2 RefSeq:Os02g0649900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

File:Fig.3 OsYSL2-3.gif

2014-05-19T16:21:46Z

Eric Fu:

File:Fig.2 OsYSL2-1.png

2014-05-19T16:21:23Z

Eric Fu:

Os02g0649900

2014-05-19T16:21:08Z

Eric Fu:

OsYSL2 was reported by researchers from Japan as a rice metal-NA transporter that is responsible for the phloem transport of iron and manganese, including the translocation of iron and manganese into the grain.
==Annotated Information==
===Function===
*OsYSL2 is an Fe-regulated metal-NA transporter that is involved in phloem transport and the translocation of mineral nutrients in grains<ref name="ref1" />. The altered expression of OsYSL2changes the localization of Fe, and that OsYSL2 is a critical Fe-nicotianamine transporter important for Fe translocation,especially in the shoots and endosperm <ref name="ref2" />.

*Based on the nucleotide sequence,OsYSL2 was predicted to encode a polypeptide of 674 amino acids containing 14 putative transmembrane domains<ref name="ref1" />. Cells expressing the OsYSL2–GFP fusion protein showed a signal in the plasma membrane, with the presence of the putative transmembrane domains shows that OsYSL2 is a transporter that is localized in the plasma membrane<ref name="ref1" />.
[[File:Fig.1 OsYSL2.gif|right|thumb|250px|'''Figure 1.''' ''Expression and seed iron (Fe) concentrations of OsYSL2i and phenotype of OsYSL2i at the early growth stage.(From reference <ref name="ref2" />).'']]

===Mutant===
*Yasuhiro Ishimaru et al. produced OsYSL2 knock-down lines using the RNAi method. Seven independent lines were generated, and the expression of endogenous OsYSL2 in T1OsYSL2i leaves of lines 3 and 5 was suppressed, as revealed by northern blot analysis(Figure 1a). Fe concentrations in the seeds were also decreased in lines 3 and 5,consistent with the suppression of OsYSL2(Figure 1b).At the early growth stage, the growth of line 5 was impaired compared with the WT, when grown under Fe-sufficient conditions. These growth defects appeared to be more significant when grown under Fe-deficient conditions (Figure 1c–e)<ref name="ref2" />.

*Yasuhiro Ishimaru et al. assessed whether the over expression of OsYSL2(OXOsYSL2) in rice plants could enhance Fe accumulation in the seeds. Constitutive over expression of OsYSL2 decreased the Fe concentration in the brown rice and shoots ,although the Fe concentration was higher in the roots of OXOsYSL2 plants. On the other hand, the Mn concentration was increased in the brown rice and decreased in the roots of OXOsYSL2 plants<ref name="ref2" />.

[[File:Fig.2 OsYSL2-1.png|right|thumb|250px|'''Figure 2.''' ''RT-PCR analysis of OsYSL2 expression in flowers and developing seeds.(From reference <ref name="ref1" />).'']]

===Expression===
*OsYSL2 was of particular interest because no detectable transcripts were present in the roots of either Fe-sufficient or Fe-deficient plants, but dramatic expression was induced in the leaves of Fe-deficient plants<ref name="ref1" />.Research by Yasuhiro Ishimaru et al. showed that the high expression of OsYSL2 in the roots under Fe deficiency was not consistent with previous study<ref name="ref2" />.
*The cell-type specificity of OsYSL2 expression was investigated using promoter (1.5 kb):b-glucuronidase (GUS) analysis.GUS staining was observed in phloem cells of the vascular bundles of leaves and leaf sheaths of Fe-sufficient rice. The phloem-specific expression of the OsYSL2 promoter suggested that OsYSL2 is involved in the phloem transport of Fe <ref name="ref1" />. OsYSL2 is expressed during reproductive development in rice. RT-PCR analysis confirmed that OsYSL2 transcripts were increased during early seed development(figure 2)<ref name="ref1" />.
*In fully mature seeds,strong OsYSL2 expression was observed in the epithelium, the vascular bundle of the scutellum, and the leaf primordium . Two days after sowing, the OsYSL2 expression level increased in the scutellum near the endosperm. Three days after sowing,OsYSL2 expression was observed in the bran and coleoptile<ref name="ref3" />.

[[File:Fig.3 OsYSL2-3.gif|right|thumb|250px|'''Figure 3.''' ''The unrooted phylogenic tree for the 18 OsYSL amino acid sequences.(From reference <ref name="ref1" />).'']]
===Evolution===
YS1-like genes in rice Our search for YS1 homologus in the Oryza sativaL. Ssp. japonica (cv. Nipponbare) rice genomic database identified
18 putative OsYSL that exhibited 36–76% sequence similarity to YS1(figure 3) <ref name="ref1" />.

==Labs working on this gene==
*Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
*Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Kawaguchi 332-0012, Japan
*National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan

==References==
<references>
<ref name="ref1"> Koike S, Inoue H, Mizuno D, et al. OsYSL2 is a rice metal‐nicotianamine transporter that is regulated by iron and expressed in the phloem[J]. The Plant Journal, 2004, 39(3): 415-424.PMID 15255870</ref>
<ref name="ref2"> Ishimaru Y, Masuda H, Bashir K, et al. Rice metal‐nicotianamine transporter, OsYSL2, is required for the long‐distance transport of iron and manganese[J]. The Plant Journal, 2010, 62(3): 379-390.PMID 20128878</ref>
<ref name="ref3"> Nozoye T, Inoue H, Takahashi M, et al. The expression of iron homeostasis-related genes during rice germination[J]. Plant molecular biology, 2007, 64(1-2): 35-47.PMID 17333504</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0649900|
Description = Similar to Iron-phytosiderophore transporter protein yellow stripe 1|
Version = NM_001054121.2 GI:297599682 GeneID:4330161|
Length = 3302 bp|
Definition = Oryza sativa Japonica Group Os02g0649900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:27058478..27061779|
CDS = 27058478..27059406,27059491..27059605,27059697..27059886,27059990..27060124,27060204..27060301 ,27061115..27061287,27061395..27061779|
GCID = <gbrowseImage1>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</cdnaseq>|
AA = <aaseq>MEAAAPEIERCDAGDVESDHDGAAAAAERVPPWREQVTARGMVA ALLIGFVYTVIIMKLALTTGIIPTLNVSAALLAFLALRGWTRAPALLLPGGGAASSSS RRRPFTRQENTVVQTCAVACYTMGFGGGFGSSLLALNRKTYELAGVSTPGNSPGSYKE PGVGWMTGFLFAISFVGLLNLLPLRKALIIDYKLTYPSGTATAVLINGFHTPQGENSA KKQVRGFLNCFGISLLWSFFQWFYTGGESCGFLQFPTFGLKAWKQTFYFDFSLTYVGA GMICSHLVNLSALFGAILSWGIMWPLISIQKGKWYPGNVPESSMTSLFGYKSFMCVAL IMGDGLYHFIKVTGITAKSLHERSNRRHAKKATDEDTFVIADMQRDEFFNKDYIPNWL AYAGYALLSIVAVIAIPIMFQQVKWYYVVVAFVLAPVLGFSNAYGTGLTDMNMSYNYG KIALFIFAAWGGRDNGVIAGLVGCGIVKQLVQVSADLMHDFKTGHLTLTSPRSMLVGQ AIGTAMGCIIAPLTFLLFYKAFDIGNPDGYWKAPYALIFRNMAILGVEGFSALPKHCL ELSAGFFAFSVLINLMRDFLPRKYRDYVPLPTAMAVPFLVGANFAIDMCVGSLIVFAW HKINSKESALLVPAVASGFICGDGIWMFPSSLLSLAKVKPPICMKFTPGS</aaseq>|
DNA = <dnaseqindica>2374..3302#2175..2289#1894..2083#1656..1790#1479..1576#493..665#1..385#atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgcgcgccccgctactacttcctgtttgatttctctccccaagtctttcttaatttgttcttgcgatggcgcctgtatataaaattgggggatatcgtgcgcgcaggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggtgtgtactttgcccacaatttttgttccgttggtactgaatatgcaatggattaaattagacctgtccctgcaaattcaggactctctggagttcctgctattatgttcgttaattgttgtgatttgctatctttcagtgaactgtttttttctctcagttttgtttcggatttttggagaatatagctcagttgttgcatgtgaaatctgtgctatattctcagttttgtgctgatattaaaaattgtgaactcactcctattttaagatgatattttacataaacaatctgttgttacatttgtaaatttgtctgatcccagtatacggataggtcatgttttcaagaaagactaggaagtttaagttacccatcagtctaccactatctgttttactttgttaggttcttgatctgctttatcttgctaacctttaaggctttaactagaactacttgttcagactccagagtaatgcacatcgtgtgcatggcatggaaacccaactaacctgcaacagttcaacaccaattgggagttctacatagtacacatcctaatatttggtatgcggcattacatttccattatccttaaccaacaagttgactaaagtgctaaaacgaaatgaacttttttgagacaaataaaatgtcatagactaaatgagacaactgtacacacgaattccctctagatactcttcttcaagtctaaaaaatttgtcattacaatctgtagtttgaagacagaaaccagttttctttgcatttgtctgtcccacctaatgatgtgcatgacgttttcaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagtatggtccaggtctcacttgtgctaaatacatcatcgcagttcgtgatatgaattattaattctacttttcatgccaggaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgtatgcattattggtcacccattgtttgtacccctactttatgcataattgcatattcgtcaggcagccagattctcctaactaccaattttttttgctgcaggttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaaggtcaggcatacaatttacctttttctcaaaatagtctgatgtgaatccataattttatatggtacagtatctctaattttctccttctcagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaaggtaaacgtgctctgcgaaattaactatttccacgttttcttccctcttatatcaccaaggtagctgaagctgagtgaattgcagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054121.2 RefSeq:Os02g0649900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

File:Fig.1 OsYSL2.gif

2014-05-19T15:58:58Z

Eric Fu:

Os02g0649900

2014-05-19T15:50:05Z

Eric Fu: /* Function */

OsYSL2 was reported by researchers from Japan as a rice metal-NA transporter that is responsible for the phloem transport of iron and manganese, including the translocation of iron and manganese into the grain.
==Annotated Information==
===Function===
OsYSL2 is an Fe-regulated metal-NA transporter that is involved in phloem transport and the translocation of mineral nutrients in grains<ref name="ref1" />. The altered expression of OsYSL2changes the localization of Fe, and that OsYSL2 is a critical Fe-nicotianamine transporter important for Fe translocation,especially in the shoots and endosperm <ref name="ref2" />.

Based on the nucleotide sequence,OsYSL2 was predicted to encode a polypeptide of 674 amino acids containing 14 putative transmembrane domains<ref name="ref1" />. Cells expressing the OsYSL2–GFP fusion protein showed a signal in the plasma membrane, with the presence of the putative transmembrane domains shows that OsYSL2 is a transporter that is localized in the plasma membrane<ref name="ref1" />.
[[File:Fig.1 OsYSL2.gif|right|thumb|250px|Fig.1 Expression and seed iron (Fe) concentrations of OsYSL2i and phenotype of OsYSL2i at the early growth stage.(From reference <ref name="ref2" />).'']]

===Mutant===
*Yasuhiro Ishimaru et al. produced OsYSL2 knock-down lines using the RNAi method. Seven independent lines were generated, and the expression of endogenous OsYSL2 in T1OsYSL2i leaves of lines 3 and 5 was suppressed, as revealed by northern blot analysis . Fe concentrations in the seeds were also decreased in lines 3 and 5,consistent with the suppression of OsYSL2(Figure 1b).At the early growth stage, the growth of line 5 was impaired compared with the WT, when grown under Fe-sufficient conditions. These growth defects appeared to be more significant when grown under Fe-deficient conditions <ref name="ref2" />.

*Yasuhiro Ishimaru et al. assessed whether the over expression of OsYSL2(OXOsYSL2) in rice plants could enhance Fe accumulation in the seeds. Constitutive over expression of OsYSL2 decreased the Fe concentration in the brown rice and shoots ,although the Fe concentration was higher in the roots of OXOsYSL2 plants. On the other hand, the Mn concentration was increased in the brown rice and decreased in the roots of OXOsYSL2 plants<ref name="ref2" />.

===Expression===
*OsYSL2 was of particular interest because no detectable transcripts were present in the roots of either Fe-sufficient or Fe-deficient plants, but dramatic expression was induced in the leaves of Fe-deficient plants<ref name="ref1" />.Research by Yasuhiro Ishimaru et al. showed that the high expression of OsYSL2 in the roots under Fe deficiency was not consistent with previous study<ref name="ref2" />.
*The cell-type specificity of OsYSL2 expression was investigated using promoter (1.5 kb):b-glucuronidase (GUS) analysis.GUS staining was observed in phloem cells of the vascular bundles of leaves and leaf sheaths of Fe-sufficient rice. The phloem-specific expression of the OsYSL2 promoter suggested that OsYSL2 is involved in the phloem transport of Fe <ref name="ref1" />. OsYSL2 is expressed during reproductive development in rice. RT-PCR analysis confirmed that OsYSL2 transcripts were increased during early seed development<ref name="ref1" />.
*In fully mature seeds,strong OsYSL2 expression was observed in the epithelium, the vascular bundle of the scutellum, and the leaf primordium . Two days after sowing, the OsYSL2 expression level increased in the scutellum near the endosperm. Three days after sowing,OsYSL2 expression was observed in the bran and coleoptile<ref name="ref3" />.

===Evolution===
YS1-like genes in rice Our search for YS1homologues in the Oryza sativaL. Ssp. japonica (cv. Nipponbare) rice genomic database identified
18 putative OsYSL that exhibited 36–76% sequence similarity to YS1 <ref name="ref1" />.

==Labs working on this gene==
*Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
*Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Kawaguchi 332-0012, Japan
*National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan

==References==
<references>
<ref name="ref1"> Koike S, Inoue H, Mizuno D, et al. OsYSL2 is a rice metal‐nicotianamine transporter that is regulated by iron and expressed in the phloem[J]. The Plant Journal, 2004, 39(3): 415-424.PMID 15255870</ref>
<ref name="ref2"> Ishimaru Y, Masuda H, Bashir K, et al. Rice metal‐nicotianamine transporter, OsYSL2, is required for the long‐distance transport of iron and manganese[J]. The Plant Journal, 2010, 62(3): 379-390.PMID 20128878</ref>
<ref name="ref3"> Nozoye T, Inoue H, Takahashi M, et al. The expression of iron homeostasis-related genes during rice germination[J]. Plant molecular biology, 2007, 64(1-2): 35-47.PMID 17333504</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0649900|
Description = Similar to Iron-phytosiderophore transporter protein yellow stripe 1|
Version = NM_001054121.2 GI:297599682 GeneID:4330161|
Length = 3302 bp|
Definition = Oryza sativa Japonica Group Os02g0649900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:27058478..27061779|
CDS = 27058478..27059406,27059491..27059605,27059697..27059886,27059990..27060124,27060204..27060301 ,27061115..27061287,27061395..27061779|
GCID = <gbrowseImage1>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</cdnaseq>|
AA = <aaseq>MEAAAPEIERCDAGDVESDHDGAAAAAERVPPWREQVTARGMVA ALLIGFVYTVIIMKLALTTGIIPTLNVSAALLAFLALRGWTRAPALLLPGGGAASSSS RRRPFTRQENTVVQTCAVACYTMGFGGGFGSSLLALNRKTYELAGVSTPGNSPGSYKE PGVGWMTGFLFAISFVGLLNLLPLRKALIIDYKLTYPSGTATAVLINGFHTPQGENSA KKQVRGFLNCFGISLLWSFFQWFYTGGESCGFLQFPTFGLKAWKQTFYFDFSLTYVGA GMICSHLVNLSALFGAILSWGIMWPLISIQKGKWYPGNVPESSMTSLFGYKSFMCVAL IMGDGLYHFIKVTGITAKSLHERSNRRHAKKATDEDTFVIADMQRDEFFNKDYIPNWL AYAGYALLSIVAVIAIPIMFQQVKWYYVVVAFVLAPVLGFSNAYGTGLTDMNMSYNYG KIALFIFAAWGGRDNGVIAGLVGCGIVKQLVQVSADLMHDFKTGHLTLTSPRSMLVGQ AIGTAMGCIIAPLTFLLFYKAFDIGNPDGYWKAPYALIFRNMAILGVEGFSALPKHCL ELSAGFFAFSVLINLMRDFLPRKYRDYVPLPTAMAVPFLVGANFAIDMCVGSLIVFAW HKINSKESALLVPAVASGFICGDGIWMFPSSLLSLAKVKPPICMKFTPGS</aaseq>|
DNA = <dnaseqindica>2374..3302#2175..2289#1894..2083#1656..1790#1479..1576#493..665#1..385#atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgcgcgccccgctactacttcctgtttgatttctctccccaagtctttcttaatttgttcttgcgatggcgcctgtatataaaattgggggatatcgtgcgcgcaggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggtgtgtactttgcccacaatttttgttccgttggtactgaatatgcaatggattaaattagacctgtccctgcaaattcaggactctctggagttcctgctattatgttcgttaattgttgtgatttgctatctttcagtgaactgtttttttctctcagttttgtttcggatttttggagaatatagctcagttgttgcatgtgaaatctgtgctatattctcagttttgtgctgatattaaaaattgtgaactcactcctattttaagatgatattttacataaacaatctgttgttacatttgtaaatttgtctgatcccagtatacggataggtcatgttttcaagaaagactaggaagtttaagttacccatcagtctaccactatctgttttactttgttaggttcttgatctgctttatcttgctaacctttaaggctttaactagaactacttgttcagactccagagtaatgcacatcgtgtgcatggcatggaaacccaactaacctgcaacagttcaacaccaattgggagttctacatagtacacatcctaatatttggtatgcggcattacatttccattatccttaaccaacaagttgactaaagtgctaaaacgaaatgaacttttttgagacaaataaaatgtcatagactaaatgagacaactgtacacacgaattccctctagatactcttcttcaagtctaaaaaatttgtcattacaatctgtagtttgaagacagaaaccagttttctttgcatttgtctgtcccacctaatgatgtgcatgacgttttcaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagtatggtccaggtctcacttgtgctaaatacatcatcgcagttcgtgatatgaattattaattctacttttcatgccaggaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgtatgcattattggtcacccattgtttgtacccctactttatgcataattgcatattcgtcaggcagccagattctcctaactaccaattttttttgctgcaggttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaaggtcaggcatacaatttacctttttctcaaaatagtctgatgtgaatccataattttatatggtacagtatctctaattttctccttctcagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaaggtaaacgtgctctgcgaaattaactatttccacgttttcttccctcttatatcaccaaggtagctgaagctgagtgaattgcagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054121.2 RefSeq:Os02g0649900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0649900

2014-05-19T13:51:39Z

Eric Fu:

OsYSL2 was reported by researchers from Japan as a rice metal-NA transporter that is responsible for the phloem transport of iron and manganese, including the translocation of iron and manganese into the grain.
==Annotated Information==
===Function===
OsYSL2 is an Fe-regulated metal-NA transporter that is involved in phloem transport and the translocation of mineral nutrients in grains<ref name="ref1" />. The altered expression of OsYSL2changes the localization of Fe, and that OsYSL2 is a critical Fe-nicotianamine transporter important for Fe translocation,especially in the shoots and endosperm <ref name="ref2" />.

Based on the nucleotide sequence,OsYSL2 was predicted to encode a polypeptide of 674 amino acids containing 14 putative transmembrane domains<ref name="ref1" />. Cells expressing the OsYSL2–GFP fusion protein showed a signal in the plasma membrane, with the presence of the putative transmembrane domains shows that OsYSL2 is a transporter that is localized in the plasma membrane<ref name="ref1" />.

===Mutant===
*Yasuhiro Ishimaru et al. produced OsYSL2 knock-down lines using the RNAi method. Seven independent lines were generated, and the expression of endogenous OsYSL2 in T1OsYSL2i leaves of lines 3 and 5 was suppressed, as revealed by northern blot analysis . Fe concentrations in the seeds were also decreased in lines 3 and 5,consistent with the suppression of OsYSL2(Figure 1b).At the early growth stage, the growth of line 5 was impaired compared with the WT, when grown under Fe-sufficient conditions. These growth defects appeared to be more significant when grown under Fe-deficient conditions <ref name="ref2" />.

*Yasuhiro Ishimaru et al. assessed whether the over expression of OsYSL2(OXOsYSL2) in rice plants could enhance Fe accumulation in the seeds. Constitutive over expression of OsYSL2 decreased the Fe concentration in the brown rice and shoots ,although the Fe concentration was higher in the roots of OXOsYSL2 plants. On the other hand, the Mn concentration was increased in the brown rice and decreased in the roots of OXOsYSL2 plants<ref name="ref2" />.

===Expression===
*OsYSL2 was of particular interest because no detectable transcripts were present in the roots of either Fe-sufficient or Fe-deficient plants, but dramatic expression was induced in the leaves of Fe-deficient plants<ref name="ref1" />.Research by Yasuhiro Ishimaru et al. showed that the high expression of OsYSL2 in the roots under Fe deficiency was not consistent with previous study<ref name="ref2" />.
*The cell-type specificity of OsYSL2 expression was investigated using promoter (1.5 kb):b-glucuronidase (GUS) analysis.GUS staining was observed in phloem cells of the vascular bundles of leaves and leaf sheaths of Fe-sufficient rice. The phloem-specific expression of the OsYSL2 promoter suggested that OsYSL2 is involved in the phloem transport of Fe <ref name="ref1" />. OsYSL2 is expressed during reproductive development in rice. RT-PCR analysis confirmed that OsYSL2 transcripts were increased during early seed development<ref name="ref1" />.
*In fully mature seeds,strong OsYSL2 expression was observed in the epithelium, the vascular bundle of the scutellum, and the leaf primordium . Two days after sowing, the OsYSL2 expression level increased in the scutellum near the endosperm. Three days after sowing,OsYSL2 expression was observed in the bran and coleoptile<ref name="ref3" />.

===Evolution===
YS1-like genes in rice Our search for YS1homologues in the Oryza sativaL. Ssp. japonica (cv. Nipponbare) rice genomic database identified
18 putative OsYSL that exhibited 36–76% sequence similarity to YS1 <ref name="ref1" />.

==Labs working on this gene==
*Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
*Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Kawaguchi 332-0012, Japan
*National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan

==References==
<references>
<ref name="ref1"> Koike S, Inoue H, Mizuno D, et al. OsYSL2 is a rice metal‐nicotianamine transporter that is regulated by iron and expressed in the phloem[J]. The Plant Journal, 2004, 39(3): 415-424.PMID 15255870</ref>
<ref name="ref2"> Ishimaru Y, Masuda H, Bashir K, et al. Rice metal‐nicotianamine transporter, OsYSL2, is required for the long‐distance transport of iron and manganese[J]. The Plant Journal, 2010, 62(3): 379-390.PMID 20128878</ref>
<ref name="ref3"> Nozoye T, Inoue H, Takahashi M, et al. The expression of iron homeostasis-related genes during rice germination[J]. Plant molecular biology, 2007, 64(1-2): 35-47.PMID 17333504</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0649900|
Description = Similar to Iron-phytosiderophore transporter protein yellow stripe 1|
Version = NM_001054121.2 GI:297599682 GeneID:4330161|
Length = 3302 bp|
Definition = Oryza sativa Japonica Group Os02g0649900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:27058478..27061779|
CDS = 27058478..27059406,27059491..27059605,27059697..27059886,27059990..27060124,27060204..27060301 ,27061115..27061287,27061395..27061779|
GCID = <gbrowseImage1>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</cdnaseq>|
AA = <aaseq>MEAAAPEIERCDAGDVESDHDGAAAAAERVPPWREQVTARGMVA ALLIGFVYTVIIMKLALTTGIIPTLNVSAALLAFLALRGWTRAPALLLPGGGAASSSS RRRPFTRQENTVVQTCAVACYTMGFGGGFGSSLLALNRKTYELAGVSTPGNSPGSYKE PGVGWMTGFLFAISFVGLLNLLPLRKALIIDYKLTYPSGTATAVLINGFHTPQGENSA KKQVRGFLNCFGISLLWSFFQWFYTGGESCGFLQFPTFGLKAWKQTFYFDFSLTYVGA GMICSHLVNLSALFGAILSWGIMWPLISIQKGKWYPGNVPESSMTSLFGYKSFMCVAL IMGDGLYHFIKVTGITAKSLHERSNRRHAKKATDEDTFVIADMQRDEFFNKDYIPNWL AYAGYALLSIVAVIAIPIMFQQVKWYYVVVAFVLAPVLGFSNAYGTGLTDMNMSYNYG KIALFIFAAWGGRDNGVIAGLVGCGIVKQLVQVSADLMHDFKTGHLTLTSPRSMLVGQ AIGTAMGCIIAPLTFLLFYKAFDIGNPDGYWKAPYALIFRNMAILGVEGFSALPKHCL ELSAGFFAFSVLINLMRDFLPRKYRDYVPLPTAMAVPFLVGANFAIDMCVGSLIVFAW HKINSKESALLVPAVASGFICGDGIWMFPSSLLSLAKVKPPICMKFTPGS</aaseq>|
DNA = <dnaseqindica>2374..3302#2175..2289#1894..2083#1656..1790#1479..1576#493..665#1..385#atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgcgcgccccgctactacttcctgtttgatttctctccccaagtctttcttaatttgttcttgcgatggcgcctgtatataaaattgggggatatcgtgcgcgcaggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggtgtgtactttgcccacaatttttgttccgttggtactgaatatgcaatggattaaattagacctgtccctgcaaattcaggactctctggagttcctgctattatgttcgttaattgttgtgatttgctatctttcagtgaactgtttttttctctcagttttgtttcggatttttggagaatatagctcagttgttgcatgtgaaatctgtgctatattctcagttttgtgctgatattaaaaattgtgaactcactcctattttaagatgatattttacataaacaatctgttgttacatttgtaaatttgtctgatcccagtatacggataggtcatgttttcaagaaagactaggaagtttaagttacccatcagtctaccactatctgttttactttgttaggttcttgatctgctttatcttgctaacctttaaggctttaactagaactacttgttcagactccagagtaatgcacatcgtgtgcatggcatggaaacccaactaacctgcaacagttcaacaccaattgggagttctacatagtacacatcctaatatttggtatgcggcattacatttccattatccttaaccaacaagttgactaaagtgctaaaacgaaatgaacttttttgagacaaataaaatgtcatagactaaatgagacaactgtacacacgaattccctctagatactcttcttcaagtctaaaaaatttgtcattacaatctgtagtttgaagacagaaaccagttttctttgcatttgtctgtcccacctaatgatgtgcatgacgttttcaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagtatggtccaggtctcacttgtgctaaatacatcatcgcagttcgtgatatgaattattaattctacttttcatgccaggaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgtatgcattattggtcacccattgtttgtacccctactttatgcataattgcatattcgtcaggcagccagattctcctaactaccaattttttttgctgcaggttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaaggtcaggcatacaatttacctttttctcaaaatagtctgatgtgaatccataattttatatggtacagtatctctaattttctccttctcagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaaggtaaacgtgctctgcgaaattaactatttccacgttttcttccctcttatatcaccaaggtagctgaagctgagtgaattgcagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054121.2 RefSeq:Os02g0649900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0649900

2014-05-19T13:16:56Z

Eric Fu:

OsYSL2 was reported by researchers from Japan as a rice metal-NA transporter that is responsible for the phloem transport of iron and manganese, including the translocation of iron and manganese into the grain.
==Annotated Information==
===Function===
OsYSL2 is an Fe-regulated metal-NA transporter that is involved in phloem transport and the translocation of mineral nutrients in grains<ref name="ref1" />. The altered expression of OsYSL2changes the localization of Fe, and that OsYSL2 is a critical Fe-nicotianamine transporter important for Fe translocation,especially in the shoots and endosperm <ref name="ref2" />.

Based on the nucleotide sequence,OsYSL2 was predicted to encode a polypeptide of 674 amino acids containing 14 putative transmembrane domains<ref name="ref1" />. Cells expressing the OsYSL2–GFP fusion protein showed a signal in the plasma membrane, with the presence of the putative transmembrane domains shows that OsYSL2 is a transporter that is localized in the plasma membrane<ref name="ref1" />.

===Mutant===
*Yasuhiro Ishimaru et al. produced OsYSL2 knock-down lines using the RNAi method. Seven independent lines were generated, and the expression of endogenous OsYSL2 in T1OsYSL2i leaves of lines 3 and 5 was suppressed, as revealed by northern blot analysis (Figure 1a) . Fe concentrations in the seeds were also decreased in lines 3 and 5,consistent with the suppression of OsYSL2(Figure 1b).At the early growth stage, the growth of line 5 was impaired compared with the WT, when grown under Fe-sufficient conditions. These growth defects appeared to be more significant when grown under Fe-deficient conditions (Figure 2c–e)<ref name="ref2" />.

*Yasuhiro Ishimaru et al. assessed whether the over expression of OsYSL2(OXOsYSL2) in rice plants could enhance Fe accumulation in the seeds. Constitutive over expression of OsYSL2 decreased the Fe concentration in the brown rice and shoots ,although the Fe concentration was higher in the roots of OXOsYSL2 plants. On the other hand, the Mn concentration was increased in the brown rice and decreased in the roots of OXOsYSL2 plants<ref name="ref2" />.

===Expression===
*OsYSL2 was of particular interest because no detectable transcripts were present in the roots of either Fe-sufficient or Fe-deficient plants, but dramatic expression was induced in the leaves of Fe-deficient plants<ref name="ref1" />.Research by Yasuhiro Ishimaru et al. showed that the high expression of OsYSL2 in the roots under Fe deficiency was not consistent with previous study<ref name="ref2" />.
*The cell-type specificity of OsYSL2 expression was investigated using promoter (1.5 kb):b-glucuronidase (GUS) analysis.GUS staining was observed in phloem cells of the vascular bundles of leaves and leaf sheaths of Fe-sufficient rice(Figure 2e–g). The phloem-specific expression of the OsYSL2 promoter suggested that OsYSL2 is involved in the phloem transport of Fe <ref name="ref1" />. OsYSL2 is expressed during reproductive development in rice. RT-PCR analysis confirmed that OsYSL2 transcripts were increased during early seed development<ref name="ref1" />.
*In fully mature seeds,strong OsYSL2 expression was observed in the epithelium, the vascular bundle of the scutellum, and the leaf primordium . Two days after sowing, the OsYSL2 expression level increased in the scutellum near the endosperm. Three days after sowing,OsYSL2 expression was observed in the bran and coleoptile<ref name="ref3" />.

===Evolution===
YS1-like genes in rice Our search for YS1homologues in the Oryza sativaL. Ssp. japonica (cv. Nipponbare) rice genomic database identified
18 putative OsYSL that exhibited 36–76% sequence similarity to YS1 (Figure 3)<ref name="ref1" />.

==Labs working on this gene==
*Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
*Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Kawaguchi 332-0012, Japan
*National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan

==References==
<references>
<ref name="ref1"> Koike S, Inoue H, Mizuno D, et al. OsYSL2 is a rice metal‐nicotianamine transporter that is regulated by iron and expressed in the phloem[J]. The Plant Journal, 2004, 39(3): 415-424.PMID 15255870</ref>
<ref name="ref2"> Ishimaru Y, Masuda H, Bashir K, et al. Rice metal‐nicotianamine transporter, OsYSL2, is required for the long‐distance transport of iron and manganese[J]. The Plant Journal, 2010, 62(3): 379-390.PMID 20128878</ref>
<ref name="ref3"> Nozoye T, Inoue H, Takahashi M, et al. The expression of iron homeostasis-related genes during rice germination[J]. Plant molecular biology, 2007, 64(1-2): 35-47.PMID 17333504</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0649900|
Description = Similar to Iron-phytosiderophore transporter protein yellow stripe 1|
Version = NM_001054121.2 GI:297599682 GeneID:4330161|
Length = 3302 bp|
Definition = Oryza sativa Japonica Group Os02g0649900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:27058478..27061779|
CDS = 27058478..27059406,27059491..27059605,27059697..27059886,27059990..27060124,27060204..27060301 ,27061115..27061287,27061395..27061779|
GCID = <gbrowseImage1>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</cdnaseq>|
AA = <aaseq>MEAAAPEIERCDAGDVESDHDGAAAAAERVPPWREQVTARGMVA ALLIGFVYTVIIMKLALTTGIIPTLNVSAALLAFLALRGWTRAPALLLPGGGAASSSS RRRPFTRQENTVVQTCAVACYTMGFGGGFGSSLLALNRKTYELAGVSTPGNSPGSYKE PGVGWMTGFLFAISFVGLLNLLPLRKALIIDYKLTYPSGTATAVLINGFHTPQGENSA KKQVRGFLNCFGISLLWSFFQWFYTGGESCGFLQFPTFGLKAWKQTFYFDFSLTYVGA GMICSHLVNLSALFGAILSWGIMWPLISIQKGKWYPGNVPESSMTSLFGYKSFMCVAL IMGDGLYHFIKVTGITAKSLHERSNRRHAKKATDEDTFVIADMQRDEFFNKDYIPNWL AYAGYALLSIVAVIAIPIMFQQVKWYYVVVAFVLAPVLGFSNAYGTGLTDMNMSYNYG KIALFIFAAWGGRDNGVIAGLVGCGIVKQLVQVSADLMHDFKTGHLTLTSPRSMLVGQ AIGTAMGCIIAPLTFLLFYKAFDIGNPDGYWKAPYALIFRNMAILGVEGFSALPKHCL ELSAGFFAFSVLINLMRDFLPRKYRDYVPLPTAMAVPFLVGANFAIDMCVGSLIVFAW HKINSKESALLVPAVASGFICGDGIWMFPSSLLSLAKVKPPICMKFTPGS</aaseq>|
DNA = <dnaseqindica>2374..3302#2175..2289#1894..2083#1656..1790#1479..1576#493..665#1..385#atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgcgcgccccgctactacttcctgtttgatttctctccccaagtctttcttaatttgttcttgcgatggcgcctgtatataaaattgggggatatcgtgcgcgcaggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggtgtgtactttgcccacaatttttgttccgttggtactgaatatgcaatggattaaattagacctgtccctgcaaattcaggactctctggagttcctgctattatgttcgttaattgttgtgatttgctatctttcagtgaactgtttttttctctcagttttgtttcggatttttggagaatatagctcagttgttgcatgtgaaatctgtgctatattctcagttttgtgctgatattaaaaattgtgaactcactcctattttaagatgatattttacataaacaatctgttgttacatttgtaaatttgtctgatcccagtatacggataggtcatgttttcaagaaagactaggaagtttaagttacccatcagtctaccactatctgttttactttgttaggttcttgatctgctttatcttgctaacctttaaggctttaactagaactacttgttcagactccagagtaatgcacatcgtgtgcatggcatggaaacccaactaacctgcaacagttcaacaccaattgggagttctacatagtacacatcctaatatttggtatgcggcattacatttccattatccttaaccaacaagttgactaaagtgctaaaacgaaatgaacttttttgagacaaataaaatgtcatagactaaatgagacaactgtacacacgaattccctctagatactcttcttcaagtctaaaaaatttgtcattacaatctgtagtttgaagacagaaaccagttttctttgcatttgtctgtcccacctaatgatgtgcatgacgttttcaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagtatggtccaggtctcacttgtgctaaatacatcatcgcagttcgtgatatgaattattaattctacttttcatgccaggaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgtatgcattattggtcacccattgtttgtacccctactttatgcataattgcatattcgtcaggcagccagattctcctaactaccaattttttttgctgcaggttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaaggtcaggcatacaatttacctttttctcaaaatagtctgatgtgaatccataattttatatggtacagtatctctaattttctccttctcagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaaggtaaacgtgctctgcgaaattaactatttccacgttttcttccctcttatatcaccaaggtagctgaagctgagtgaattgcagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054121.2 RefSeq:Os02g0649900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

File:OsYSL2-3.gif

2014-05-19T12:53:06Z

Eric Fu:

File:GUS staining.gif

2014-05-19T12:48:34Z

Eric Fu:

File:Mutant.gif

2014-05-19T12:43:43Z

Eric Fu: uploaded a new version of "File:Mutant.gif"

Os02g0649900

2014-05-19T12:42:52Z

Eric Fu:

OsYSL2 was reported by researchers from Japan as a rice metal-NA transporter that is responsible for the phloem transport of iron and manganese, including the translocation of iron and manganese into the grain.
==Annotated Information==
===Function===
OsYSL2 is an Fe-regulated metal-NA transporter that is involved in phloem transport and the translocation of mineral nutrients in grains<ref name="ref1" />. The altered expression of OsYSL2changes the localization of Fe, and that OsYSL2 is a critical Fe-nicotianamine transporter important for Fe translocation,especially in the shoots and endosperm <ref name="ref2" />.
Based on the nucleotide sequence,OsYSL2 was predicted to encode a polypeptide of 674 amino acids containing 14 putative transmembrane domains<ref name="ref1" />. Cells expressing the OsYSL2–GFP fusion protein showed a signal in the plasma membrane, with the presence of the putative transmembrane domains shows that OsYSL2 is a transporter that is localized in the plasma membrane<ref name="ref1" />.

===Mutant===
*Yasuhiro Ishimaru et al. produced OsYSL2 knock-down lines using the RNAi method. Seven independent lines were generated, and the expression of endogenous OsYSL2 in T1OsYSL2i leaves of lines 3 and 5 was suppressed, as revealed by northern blot analysis (Figure 1a) . Fe concentrations in the seeds were also decreased in lines 3 and 5,consistent with the suppression of OsYSL2(Figure 1b).At the early growth stage, the growth of line 5 was impaired compared with the WT, when grown under Fe-sufficient conditions. These growth defects appeared to be more significant when grown under Fe-deficient conditions (Figure 2c–e)<ref name="ref2" />.

*Yasuhiro Ishimaru et al. assessed whether the over expression of OsYSL2(OXOsYSL2) in rice plants could enhance Fe accumulation in the seeds. Constitutive over expression of OsYSL2 decreased the Fe concentration in the brown rice and shoots ,although the Fe concentration was higher in the roots of OXOsYSL2 plants. On the other hand, the Mn concentration was increased in the brown rice and decreased in the roots of OXOsYSL2 plants<ref name="ref2" />.

===Expression===
*OsYSL2 was of particular interest because no detectable transcripts were present in the roots of either Fe-sufficient or Fe-deficient plants, but dramatic expression was induced in the leaves of Fe-deficient plants<ref name="ref1" />.Research by Yasuhiro Ishimaru et al. showed that the high expression of OsYSL2 in the roots under Fe deficiency was not consistent with previous study<ref name="ref2" />.
*The cell-type specificity of OsYSL2 expression was investigated using promoter (1.5 kb):b-glucuronidase (GUS) analysis.GUS staining was observed in phloem cells of the vascular bundles of leaves and leaf sheaths of Fe-sufficient rice(Figure 2e–g). The phloem-specific expression of the OsYSL2 promoter suggested that OsYSL2 is involved in the phloem transport of Fe <ref name="ref1" />. OsYSL2 is expressed during reproductive development in rice. RT-PCR analysis confirmed that OsYSL2 transcripts were increased during early seed development<ref name="ref1" />.
*In fully mature seeds,strong OsYSL2 expression was observed in the epithelium, the vascular bundle of the scutellum, and the leaf primordium . Two days after sowing, the OsYSL2 expression level increased in the scutellum near the endosperm. Three days after sowing,OsYSL2 expression was observed in the bran and coleoptile<ref name="ref3" />.

===Evolution===
YS1-like genes in rice Our search for YS1homologues in the Oryza sativaL. Ssp. japonica (cv. Nipponbare) rice genomic database identified
18 putative OsYSL that exhibited 36–76% sequence similarity to YS1 (Figure 3)<ref name="ref1" />.

==Labs working on this gene==
*Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
*Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Kawaguchi 332-0012, Japan
*National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan

==References==
<references>
<ref name="ref1"> Koike S, Inoue H, Mizuno D, et al. OsYSL2 is a rice metal‐nicotianamine transporter that is regulated by iron and expressed in the phloem[J]. The Plant Journal, 2004, 39(3): 415-424.PMID 15255870</ref>
<ref name="ref2"> Ishimaru Y, Masuda H, Bashir K, et al. Rice metal‐nicotianamine transporter, OsYSL2, is required for the long‐distance transport of iron and manganese[J]. The Plant Journal, 2010, 62(3): 379-390.PMID 20128878</ref>
<ref name="ref3"> Nozoye T, Inoue H, Takahashi M, et al. The expression of iron homeostasis-related genes during rice germination[J]. Plant molecular biology, 2007, 64(1-2): 35-47.PMID 17333504</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0649900|
Description = Similar to Iron-phytosiderophore transporter protein yellow stripe 1|
Version = NM_001054121.2 GI:297599682 GeneID:4330161|
Length = 3302 bp|
Definition = Oryza sativa Japonica Group Os02g0649900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:27058478..27061779|
CDS = 27058478..27059406,27059491..27059605,27059697..27059886,27059990..27060124,27060204..27060301 ,27061115..27061287,27061395..27061779|
GCID = <gbrowseImage1>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</cdnaseq>|
AA = <aaseq>MEAAAPEIERCDAGDVESDHDGAAAAAERVPPWREQVTARGMVA ALLIGFVYTVIIMKLALTTGIIPTLNVSAALLAFLALRGWTRAPALLLPGGGAASSSS RRRPFTRQENTVVQTCAVACYTMGFGGGFGSSLLALNRKTYELAGVSTPGNSPGSYKE PGVGWMTGFLFAISFVGLLNLLPLRKALIIDYKLTYPSGTATAVLINGFHTPQGENSA KKQVRGFLNCFGISLLWSFFQWFYTGGESCGFLQFPTFGLKAWKQTFYFDFSLTYVGA GMICSHLVNLSALFGAILSWGIMWPLISIQKGKWYPGNVPESSMTSLFGYKSFMCVAL IMGDGLYHFIKVTGITAKSLHERSNRRHAKKATDEDTFVIADMQRDEFFNKDYIPNWL AYAGYALLSIVAVIAIPIMFQQVKWYYVVVAFVLAPVLGFSNAYGTGLTDMNMSYNYG KIALFIFAAWGGRDNGVIAGLVGCGIVKQLVQVSADLMHDFKTGHLTLTSPRSMLVGQ AIGTAMGCIIAPLTFLLFYKAFDIGNPDGYWKAPYALIFRNMAILGVEGFSALPKHCL ELSAGFFAFSVLINLMRDFLPRKYRDYVPLPTAMAVPFLVGANFAIDMCVGSLIVFAW HKINSKESALLVPAVASGFICGDGIWMFPSSLLSLAKVKPPICMKFTPGS</aaseq>|
DNA = <dnaseqindica>2374..3302#2175..2289#1894..2083#1656..1790#1479..1576#493..665#1..385#atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgcgcgccccgctactacttcctgtttgatttctctccccaagtctttcttaatttgttcttgcgatggcgcctgtatataaaattgggggatatcgtgcgcgcaggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggtgtgtactttgcccacaatttttgttccgttggtactgaatatgcaatggattaaattagacctgtccctgcaaattcaggactctctggagttcctgctattatgttcgttaattgttgtgatttgctatctttcagtgaactgtttttttctctcagttttgtttcggatttttggagaatatagctcagttgttgcatgtgaaatctgtgctatattctcagttttgtgctgatattaaaaattgtgaactcactcctattttaagatgatattttacataaacaatctgttgttacatttgtaaatttgtctgatcccagtatacggataggtcatgttttcaagaaagactaggaagtttaagttacccatcagtctaccactatctgttttactttgttaggttcttgatctgctttatcttgctaacctttaaggctttaactagaactacttgttcagactccagagtaatgcacatcgtgtgcatggcatggaaacccaactaacctgcaacagttcaacaccaattgggagttctacatagtacacatcctaatatttggtatgcggcattacatttccattatccttaaccaacaagttgactaaagtgctaaaacgaaatgaacttttttgagacaaataaaatgtcatagactaaatgagacaactgtacacacgaattccctctagatactcttcttcaagtctaaaaaatttgtcattacaatctgtagtttgaagacagaaaccagttttctttgcatttgtctgtcccacctaatgatgtgcatgacgttttcaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagtatggtccaggtctcacttgtgctaaatacatcatcgcagttcgtgatatgaattattaattctacttttcatgccaggaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgtatgcattattggtcacccattgtttgtacccctactttatgcataattgcatattcgtcaggcagccagattctcctaactaccaattttttttgctgcaggttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaaggtcaggcatacaatttacctttttctcaaaatagtctgatgtgaatccataattttatatggtacagtatctctaattttctccttctcagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaaggtaaacgtgctctgcgaaattaactatttccacgttttcttccctcttatatcaccaaggtagctgaagctgagtgaattgcagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054121.2 RefSeq:Os02g0649900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

File:Mutant.gif

2014-05-19T12:40:16Z

Eric Fu: uploaded a new version of "File:Mutant.gif"

File:Mutant.gif

2014-05-19T12:37:53Z

Eric Fu:

Os02g0649900

2014-05-19T10:10:56Z

Eric Fu:

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsYSL2 is an Fe-regulated metal-NA transporter that is involved in phloem transport and the translocation of mineral nutrients in grains<ref name="ref1" />. The altered expression of OsYSL2changes the localization of Fe, and that OsYSL2 is a critical Fe-nicotianamine transporter important for Fe translocation,especially in the shoots and endosperm <ref name="ref2" />.
Based on the nucleotide sequence,OsYSL2 was predicted to encode a polypeptide of 674 amino acids containing 14 putative transmembrane domains(figure 1a)<ref name="ref1" />. Cells expressing the OsYSL2–GFP fusion protein showed a signal in the plasma membrane, with the presence of the putative transmembrane domains shows that OsYSL2 is a transporter that is localized in the plasma membrane(figure 1b)<ref name="ref1" />.

===Mutant===
*Yasuhiro Ishimaru et al. produced OsYSL2 knock-down lines using the RNAi method. Seven independent lines were generated, and the expression of endogenous OsYSL2 in T1OsYSL2i leaves of lines 3 and 5 was suppressed, as revealed by northern blot analysis (Figure 2a) . Fe concentrations in the seeds were also decreased in lines 3 and 5,consistent with the suppression of OsYSL2(Figure 2b).At the early growth stage, the growth of line 5 was impaired compared with the WT, when grown under Fe-sufficient conditions. These growth defects appeared to be more significant when grown under Fe-deficient conditions (Figure 2c–e)<ref name="ref2" />.

*Yasuhiro Ishimaru et al. assessed whether the over expression of OsYSL2(OXOsYSL2) in rice plants could enhance Fe accumulation in the seeds. Constitutive over expression of OsYSL2 decreased the Fe concentration in the brown rice and shoots ,although the Fe concentration was higher in the roots of OXOsYSL2 plants. On the other hand, the Mn concentration was increased in the brown rice and decreased in the roots of OXOsYSL2 plants<ref name="ref2" />.

===Expression===
*OsYSL2 was of particular interest because no detectable transcripts were present in the roots of either Fe-sufficient or Fe-deficient plants, but dramatic expression was induced in the leaves of Fe-deficient plants<ref name="ref1" />.Research by Yasuhiro Ishimaru et al. showed that the high expression of OsYSL2 in the roots under Fe deficiency was not consistent with previous study<ref name="ref2" />.
*The cell-type specificity of OsYSL2 expression was investigated using promoter (1.5 kb):b-glucuronidase (GUS) analysis.GUS staining was observed in phloem cells of the vascular bundles of leaves and leaf sheaths of Fe-sufficient rice(Figure 3e–g). The phloem-specific expression of the OsYSL2 promoter suggested that OsYSL2 is involved in the phloem transport of Fe <ref name="ref1" />. OsYSL2 is expressed during reproductive development in rice. RT-PCR analysis confirmed that OsYSL2 transcripts were increased during early seed development<ref name="ref1" />.
*In fully mature seeds,strong OsYSL2 expression was observed in the epithelium, the vascular bundle of the scutellum, and the leaf primordium . Two days after sowing, the OsYSL2 expression level increased in the scutellum near the endosperm. Three days after sowing,OsYSL2 expression was observed in the bran and coleoptile<ref name="ref3" />.

===Evolution===
YS1-like genes in rice Our search for YS1homologues in the Oryza sativaL. Ssp. japonica (cv. Nipponbare) rice genomic database identified
18 putative OsYSL that exhibited 36–76% sequence similarity to YS1 (Figure 4)<ref name="ref1" />.

==Labs working on this gene==
*Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
*Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Kawaguchi 332-0012, Japan
*National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan

==References==
<references>
<ref name="ref1"> Koike S, Inoue H, Mizuno D, et al. OsYSL2 is a rice metal‐nicotianamine transporter that is regulated by iron and expressed in the phloem[J]. The Plant Journal, 2004, 39(3): 415-424.PMID 15255870</ref>
<ref name="ref2"> Ishimaru Y, Masuda H, Bashir K, et al. Rice metal‐nicotianamine transporter, OsYSL2, is required for the long‐distance transport of iron and manganese[J]. The Plant Journal, 2010, 62(3): 379-390.PMID 20128878</ref>
<ref name="ref3"> Nozoye T, Inoue H, Takahashi M, et al. The expression of iron homeostasis-related genes during rice germination[J]. Plant molecular biology, 2007, 64(1-2): 35-47.PMID 17333504</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0649900|
Description = Similar to Iron-phytosiderophore transporter protein yellow stripe 1|
Version = NM_001054121.2 GI:297599682 GeneID:4330161|
Length = 3302 bp|
Definition = Oryza sativa Japonica Group Os02g0649900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:27058478..27061779|
CDS = 27058478..27059406,27059491..27059605,27059697..27059886,27059990..27060124,27060204..27060301 ,27061115..27061287,27061395..27061779|
GCID = <gbrowseImage1>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</cdnaseq>|
AA = <aaseq>MEAAAPEIERCDAGDVESDHDGAAAAAERVPPWREQVTARGMVA ALLIGFVYTVIIMKLALTTGIIPTLNVSAALLAFLALRGWTRAPALLLPGGGAASSSS RRRPFTRQENTVVQTCAVACYTMGFGGGFGSSLLALNRKTYELAGVSTPGNSPGSYKE PGVGWMTGFLFAISFVGLLNLLPLRKALIIDYKLTYPSGTATAVLINGFHTPQGENSA KKQVRGFLNCFGISLLWSFFQWFYTGGESCGFLQFPTFGLKAWKQTFYFDFSLTYVGA GMICSHLVNLSALFGAILSWGIMWPLISIQKGKWYPGNVPESSMTSLFGYKSFMCVAL IMGDGLYHFIKVTGITAKSLHERSNRRHAKKATDEDTFVIADMQRDEFFNKDYIPNWL AYAGYALLSIVAVIAIPIMFQQVKWYYVVVAFVLAPVLGFSNAYGTGLTDMNMSYNYG KIALFIFAAWGGRDNGVIAGLVGCGIVKQLVQVSADLMHDFKTGHLTLTSPRSMLVGQ AIGTAMGCIIAPLTFLLFYKAFDIGNPDGYWKAPYALIFRNMAILGVEGFSALPKHCL ELSAGFFAFSVLINLMRDFLPRKYRDYVPLPTAMAVPFLVGANFAIDMCVGSLIVFAW HKINSKESALLVPAVASGFICGDGIWMFPSSLLSLAKVKPPICMKFTPGS</aaseq>|
DNA = <dnaseqindica>2374..3302#2175..2289#1894..2083#1656..1790#1479..1576#493..665#1..385#atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgcgcgccccgctactacttcctgtttgatttctctccccaagtctttcttaatttgttcttgcgatggcgcctgtatataaaattgggggatatcgtgcgcgcaggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggtgtgtactttgcccacaatttttgttccgttggtactgaatatgcaatggattaaattagacctgtccctgcaaattcaggactctctggagttcctgctattatgttcgttaattgttgtgatttgctatctttcagtgaactgtttttttctctcagttttgtttcggatttttggagaatatagctcagttgttgcatgtgaaatctgtgctatattctcagttttgtgctgatattaaaaattgtgaactcactcctattttaagatgatattttacataaacaatctgttgttacatttgtaaatttgtctgatcccagtatacggataggtcatgttttcaagaaagactaggaagtttaagttacccatcagtctaccactatctgttttactttgttaggttcttgatctgctttatcttgctaacctttaaggctttaactagaactacttgttcagactccagagtaatgcacatcgtgtgcatggcatggaaacccaactaacctgcaacagttcaacaccaattgggagttctacatagtacacatcctaatatttggtatgcggcattacatttccattatccttaaccaacaagttgactaaagtgctaaaacgaaatgaacttttttgagacaaataaaatgtcatagactaaatgagacaactgtacacacgaattccctctagatactcttcttcaagtctaaaaaatttgtcattacaatctgtagtttgaagacagaaaccagttttctttgcatttgtctgtcccacctaatgatgtgcatgacgttttcaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagtatggtccaggtctcacttgtgctaaatacatcatcgcagttcgtgatatgaattattaattctacttttcatgccaggaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgtatgcattattggtcacccattgtttgtacccctactttatgcataattgcatattcgtcaggcagccagattctcctaactaccaattttttttgctgcaggttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaaggtcaggcatacaatttacctttttctcaaaatagtctgatgtgaatccataattttatatggtacagtatctctaattttctccttctcagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaaggtaaacgtgctctgcgaaattaactatttccacgttttcttccctcttatatcaccaaggtagctgaagctgagtgaattgcagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054121.2 RefSeq:Os02g0649900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0649900

2014-05-19T10:04:01Z

Eric Fu: /* Expression */

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsYSL2 is an Fe-regulated metal-NA transporter that is involved in phloem transport and the translocation of mineral nutrients in grains<ref name="ref1" />. The altered expression of OsYSL2changes the localization of Fe, and that OsYSL2 is a critical Fe-nicotianamine transporter important for Fe translocation,especially in the shoots and endosperm <ref name="ref2" />.Based on the nucleotide sequence,OsYSL2 was predicted to encode a polypeptide of 674 amino acids containing 14 putative transmembrane domains(figure 1a)<ref name="ref1" />. Cells expressing the OsYSL2–GFP fusion protein showed a signal in the plasma membrane, with the presence of the putative transmembrane domains shows that OsYSL2 is a transporter that is localized in the plasma membrane(figure 1b)<ref name="ref1" />.

===Expression===
*OsYSL2 was of particular interest because no detectable transcripts were present in the roots of either Fe-sufficient or Fe-deficient plants, but dramatic expression was induced in the leaves of Fe-deficient plants<ref name="ref1" />.Research by Yasuhiro Ishimaru et al. showed that the high expression of OsYSL2 in the roots under Fe deficiency was not consistent with previous study<ref name="ref2" />.
*The cell-type specificity of OsYSL2 expression was investigated using promoter (1.5 kb):b-glucuronidase (GUS) analysis.GUS staining was observed in phloem cells of the vascular bundles of leaves and leaf sheaths of Fe-sufficient rice(Figure 3e–g). The phloem-specific expression of the OsYSL2 promoter suggested that OsYSL2 is involved in the phloem transport of Fe <ref name="ref1" />. OsYSL2 is expressed during reproductive development in rice. RT-PCR analysis confirmed that OsYSL2 transcripts were increased during early seed development<ref name="ref1" />.
*In fully mature seeds,strong OsYSL2 expression was observed in the epithelium, the vascular bundle of the scutellum, and the leaf primordium . Two days after sowing, the OsYSL2 expression level increased in the scutellum near the endosperm. Three days after sowing,OsYSL2 expression was observed in the bran and coleoptile<ref name="ref3" />.

===Evolution===
YS1-like genes in rice Our search for YS1homologues in the Oryza sativaL. Ssp. japonica (cv. Nipponbare) rice genomic database identified
18 putative OsYSL that exhibited 36–76% sequence similarity to YS1 (Figure 4)<ref name="ref1" />.

==Labs working on this gene==
*Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
*Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Kawaguchi 332-0012, Japan
*National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan

==References==
<references>
<ref name="ref1"> Koike S, Inoue H, Mizuno D, et al. OsYSL2 is a rice metal‐nicotianamine transporter that is regulated by iron and expressed in the phloem[J]. The Plant Journal, 2004, 39(3): 415-424.PMID 15255870</ref>
<ref name="ref2"> Ishimaru Y, Masuda H, Bashir K, et al. Rice metal‐nicotianamine transporter, OsYSL2, is required for the long‐distance transport of iron and manganese[J]. The Plant Journal, 2010, 62(3): 379-390.PMID 20128878</ref>
<ref name="ref3"> Nozoye T, Inoue H, Takahashi M, et al. The expression of iron homeostasis-related genes during rice germination[J]. Plant molecular biology, 2007, 64(1-2): 35-47.PMID 17333504</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0649900|
Description = Similar to Iron-phytosiderophore transporter protein yellow stripe 1|
Version = NM_001054121.2 GI:297599682 GeneID:4330161|
Length = 3302 bp|
Definition = Oryza sativa Japonica Group Os02g0649900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:27058478..27061779|
CDS = 27058478..27059406,27059491..27059605,27059697..27059886,27059990..27060124,27060204..27060301 ,27061115..27061287,27061395..27061779|
GCID = <gbrowseImage1>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</cdnaseq>|
AA = <aaseq>MEAAAPEIERCDAGDVESDHDGAAAAAERVPPWREQVTARGMVA ALLIGFVYTVIIMKLALTTGIIPTLNVSAALLAFLALRGWTRAPALLLPGGGAASSSS RRRPFTRQENTVVQTCAVACYTMGFGGGFGSSLLALNRKTYELAGVSTPGNSPGSYKE PGVGWMTGFLFAISFVGLLNLLPLRKALIIDYKLTYPSGTATAVLINGFHTPQGENSA KKQVRGFLNCFGISLLWSFFQWFYTGGESCGFLQFPTFGLKAWKQTFYFDFSLTYVGA GMICSHLVNLSALFGAILSWGIMWPLISIQKGKWYPGNVPESSMTSLFGYKSFMCVAL IMGDGLYHFIKVTGITAKSLHERSNRRHAKKATDEDTFVIADMQRDEFFNKDYIPNWL AYAGYALLSIVAVIAIPIMFQQVKWYYVVVAFVLAPVLGFSNAYGTGLTDMNMSYNYG KIALFIFAAWGGRDNGVIAGLVGCGIVKQLVQVSADLMHDFKTGHLTLTSPRSMLVGQ AIGTAMGCIIAPLTFLLFYKAFDIGNPDGYWKAPYALIFRNMAILGVEGFSALPKHCL ELSAGFFAFSVLINLMRDFLPRKYRDYVPLPTAMAVPFLVGANFAIDMCVGSLIVFAW HKINSKESALLVPAVASGFICGDGIWMFPSSLLSLAKVKPPICMKFTPGS</aaseq>|
DNA = <dnaseqindica>2374..3302#2175..2289#1894..2083#1656..1790#1479..1576#493..665#1..385#atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgcgcgccccgctactacttcctgtttgatttctctccccaagtctttcttaatttgttcttgcgatggcgcctgtatataaaattgggggatatcgtgcgcgcaggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggtgtgtactttgcccacaatttttgttccgttggtactgaatatgcaatggattaaattagacctgtccctgcaaattcaggactctctggagttcctgctattatgttcgttaattgttgtgatttgctatctttcagtgaactgtttttttctctcagttttgtttcggatttttggagaatatagctcagttgttgcatgtgaaatctgtgctatattctcagttttgtgctgatattaaaaattgtgaactcactcctattttaagatgatattttacataaacaatctgttgttacatttgtaaatttgtctgatcccagtatacggataggtcatgttttcaagaaagactaggaagtttaagttacccatcagtctaccactatctgttttactttgttaggttcttgatctgctttatcttgctaacctttaaggctttaactagaactacttgttcagactccagagtaatgcacatcgtgtgcatggcatggaaacccaactaacctgcaacagttcaacaccaattgggagttctacatagtacacatcctaatatttggtatgcggcattacatttccattatccttaaccaacaagttgactaaagtgctaaaacgaaatgaacttttttgagacaaataaaatgtcatagactaaatgagacaactgtacacacgaattccctctagatactcttcttcaagtctaaaaaatttgtcattacaatctgtagtttgaagacagaaaccagttttctttgcatttgtctgtcccacctaatgatgtgcatgacgttttcaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagtatggtccaggtctcacttgtgctaaatacatcatcgcagttcgtgatatgaattattaattctacttttcatgccaggaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgtatgcattattggtcacccattgtttgtacccctactttatgcataattgcatattcgtcaggcagccagattctcctaactaccaattttttttgctgcaggttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaaggtcaggcatacaatttacctttttctcaaaatagtctgatgtgaatccataattttatatggtacagtatctctaattttctccttctcagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaaggtaaacgtgctctgcgaaattaactatttccacgttttcttccctcttatatcaccaaggtagctgaagctgagtgaattgcagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054121.2 RefSeq:Os02g0649900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0649900

2014-05-19T10:00:29Z

Eric Fu: /* References */

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsYSL2 is an Fe-regulated metal-NA transporter that is involved in phloem transport and the translocation of mineral nutrients in grains<ref name="ref1" />. The altered expression of OsYSL2changes the localization of Fe, and that OsYSL2 is a critical Fe-nicotianamine transporter important for Fe translocation,especially in the shoots and endosperm <ref name="ref2" />.Based on the nucleotide sequence,OsYSL2 was predicted to encode a polypeptide of 674 amino acids containing 14 putative transmembrane domains(figure 1a)<ref name="ref1" />. Cells expressing the OsYSL2–GFP fusion protein showed a signal in the plasma membrane, with the presence of the putative transmembrane domains shows that OsYSL2 is a transporter that is localized in the plasma membrane(figure 1b)<ref name="ref1" />.

===Expression===
OsYSL2 was of particular interest because no detectable transcripts were present in the roots of either Fe-sufficient or Fe-deficient plants, but dramatic expression was induced in the leaves of Fe-deficient plants<ref name="ref1" />.Research by Yasuhiro Ishimaru et al. showed that the high expression of OsYSL2 in the roots under Fe deficiency was not consistent with previous study<ref name="ref2" />. The cell-type specificity of OsYSL2 expression was investigated using promoter (1.5 kb):b-glucuronidase (GUS) analysis.GUS staining was observed in phloem cells of the vascular bundles of leaves and leaf sheaths of Fe-sufficient rice(Figure 3e–g). The phloem-specific expression of the OsYSL2 promoter suggested that OsYSL2 is involved in the phloem transport of Fe <ref name="ref1" />. OsYSL2 is expressed during reproductive development in rice. RT-PCR analysis confirmed that OsYSL2 transcripts were increased during early seed development<ref name="ref1" />.
In fully mature seeds,strong OsYSL2 expression was observed in the epithelium, the vascular bundle of the scutellum, and the leaf primordium . Two days after sowing, the OsYSL2 expression level increased in the scutellum near the endosperm. Three days after sowing,OsYSL2 expression was observed in the bran and coleoptile<ref name="ref3" />.

===Evolution===
YS1-like genes in rice Our search for YS1homologues in the Oryza sativaL. Ssp. japonica (cv. Nipponbare) rice genomic database identified
18 putative OsYSL that exhibited 36–76% sequence similarity to YS1 (Figure 4)<ref name="ref1" />.

==Labs working on this gene==
*Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
*Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Kawaguchi 332-0012, Japan
*National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan

==References==
<references>
<ref name="ref1"> Koike S, Inoue H, Mizuno D, et al. OsYSL2 is a rice metal‐nicotianamine transporter that is regulated by iron and expressed in the phloem[J]. The Plant Journal, 2004, 39(3): 415-424.PMID 15255870</ref>
<ref name="ref2"> Ishimaru Y, Masuda H, Bashir K, et al. Rice metal‐nicotianamine transporter, OsYSL2, is required for the long‐distance transport of iron and manganese[J]. The Plant Journal, 2010, 62(3): 379-390.PMID 20128878</ref>
<ref name="ref3"> Nozoye T, Inoue H, Takahashi M, et al. The expression of iron homeostasis-related genes during rice germination[J]. Plant molecular biology, 2007, 64(1-2): 35-47.PMID 17333504</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0649900|
Description = Similar to Iron-phytosiderophore transporter protein yellow stripe 1|
Version = NM_001054121.2 GI:297599682 GeneID:4330161|
Length = 3302 bp|
Definition = Oryza sativa Japonica Group Os02g0649900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:27058478..27061779|
CDS = 27058478..27059406,27059491..27059605,27059697..27059886,27059990..27060124,27060204..27060301 ,27061115..27061287,27061395..27061779|
GCID = <gbrowseImage1>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</cdnaseq>|
AA = <aaseq>MEAAAPEIERCDAGDVESDHDGAAAAAERVPPWREQVTARGMVA ALLIGFVYTVIIMKLALTTGIIPTLNVSAALLAFLALRGWTRAPALLLPGGGAASSSS RRRPFTRQENTVVQTCAVACYTMGFGGGFGSSLLALNRKTYELAGVSTPGNSPGSYKE PGVGWMTGFLFAISFVGLLNLLPLRKALIIDYKLTYPSGTATAVLINGFHTPQGENSA KKQVRGFLNCFGISLLWSFFQWFYTGGESCGFLQFPTFGLKAWKQTFYFDFSLTYVGA GMICSHLVNLSALFGAILSWGIMWPLISIQKGKWYPGNVPESSMTSLFGYKSFMCVAL IMGDGLYHFIKVTGITAKSLHERSNRRHAKKATDEDTFVIADMQRDEFFNKDYIPNWL AYAGYALLSIVAVIAIPIMFQQVKWYYVVVAFVLAPVLGFSNAYGTGLTDMNMSYNYG KIALFIFAAWGGRDNGVIAGLVGCGIVKQLVQVSADLMHDFKTGHLTLTSPRSMLVGQ AIGTAMGCIIAPLTFLLFYKAFDIGNPDGYWKAPYALIFRNMAILGVEGFSALPKHCL ELSAGFFAFSVLINLMRDFLPRKYRDYVPLPTAMAVPFLVGANFAIDMCVGSLIVFAW HKINSKESALLVPAVASGFICGDGIWMFPSSLLSLAKVKPPICMKFTPGS</aaseq>|
DNA = <dnaseqindica>2374..3302#2175..2289#1894..2083#1656..1790#1479..1576#493..665#1..385#atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgcgcgccccgctactacttcctgtttgatttctctccccaagtctttcttaatttgttcttgcgatggcgcctgtatataaaattgggggatatcgtgcgcgcaggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggtgtgtactttgcccacaatttttgttccgttggtactgaatatgcaatggattaaattagacctgtccctgcaaattcaggactctctggagttcctgctattatgttcgttaattgttgtgatttgctatctttcagtgaactgtttttttctctcagttttgtttcggatttttggagaatatagctcagttgttgcatgtgaaatctgtgctatattctcagttttgtgctgatattaaaaattgtgaactcactcctattttaagatgatattttacataaacaatctgttgttacatttgtaaatttgtctgatcccagtatacggataggtcatgttttcaagaaagactaggaagtttaagttacccatcagtctaccactatctgttttactttgttaggttcttgatctgctttatcttgctaacctttaaggctttaactagaactacttgttcagactccagagtaatgcacatcgtgtgcatggcatggaaacccaactaacctgcaacagttcaacaccaattgggagttctacatagtacacatcctaatatttggtatgcggcattacatttccattatccttaaccaacaagttgactaaagtgctaaaacgaaatgaacttttttgagacaaataaaatgtcatagactaaatgagacaactgtacacacgaattccctctagatactcttcttcaagtctaaaaaatttgtcattacaatctgtagtttgaagacagaaaccagttttctttgcatttgtctgtcccacctaatgatgtgcatgacgttttcaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagtatggtccaggtctcacttgtgctaaatacatcatcgcagttcgtgatatgaattattaattctacttttcatgccaggaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgtatgcattattggtcacccattgtttgtacccctactttatgcataattgcatattcgtcaggcagccagattctcctaactaccaattttttttgctgcaggttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaaggtcaggcatacaatttacctttttctcaaaatagtctgatgtgaatccataattttatatggtacagtatctctaattttctccttctcagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaaggtaaacgtgctctgcgaaattaactatttccacgttttcttccctcttatatcaccaaggtagctgaagctgagtgaattgcagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054121.2 RefSeq:Os02g0649900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0649900

2014-05-19T09:57:35Z

Eric Fu: /* References */

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsYSL2 is an Fe-regulated metal-NA transporter that is involved in phloem transport and the translocation of mineral nutrients in grains<ref name="ref1" />. The altered expression of OsYSL2changes the localization of Fe, and that OsYSL2 is a critical Fe-nicotianamine transporter important for Fe translocation,especially in the shoots and endosperm <ref name="ref2" />.Based on the nucleotide sequence,OsYSL2 was predicted to encode a polypeptide of 674 amino acids containing 14 putative transmembrane domains(figure 1a)<ref name="ref1" />. Cells expressing the OsYSL2–GFP fusion protein showed a signal in the plasma membrane, with the presence of the putative transmembrane domains shows that OsYSL2 is a transporter that is localized in the plasma membrane(figure 1b)<ref name="ref1" />.

===Expression===
OsYSL2 was of particular interest because no detectable transcripts were present in the roots of either Fe-sufficient or Fe-deficient plants, but dramatic expression was induced in the leaves of Fe-deficient plants<ref name="ref1" />.Research by Yasuhiro Ishimaru et al. showed that the high expression of OsYSL2 in the roots under Fe deficiency was not consistent with previous study<ref name="ref2" />. The cell-type specificity of OsYSL2 expression was investigated using promoter (1.5 kb):b-glucuronidase (GUS) analysis.GUS staining was observed in phloem cells of the vascular bundles of leaves and leaf sheaths of Fe-sufficient rice(Figure 3e–g). The phloem-specific expression of the OsYSL2 promoter suggested that OsYSL2 is involved in the phloem transport of Fe <ref name="ref1" />. OsYSL2 is expressed during reproductive development in rice. RT-PCR analysis confirmed that OsYSL2 transcripts were increased during early seed development<ref name="ref1" />.
In fully mature seeds,strong OsYSL2 expression was observed in the epithelium, the vascular bundle of the scutellum, and the leaf primordium . Two days after sowing, the OsYSL2 expression level increased in the scutellum near the endosperm. Three days after sowing,OsYSL2 expression was observed in the bran and coleoptile<ref name="ref3" />.

===Evolution===
YS1-like genes in rice Our search for YS1homologues in the Oryza sativaL. Ssp. japonica (cv. Nipponbare) rice genomic database identified
18 putative OsYSL that exhibited 36–76% sequence similarity to YS1 (Figure 4)<ref name="ref1" />.

==Labs working on this gene==
*Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
*Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Kawaguchi 332-0012, Japan
*National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan

==References==
<references>
<ref name="ref1"> Koike S, Inoue H, Mizuno D, et al. OsYSL2 is a rice metal‐nicotianamine transporter that is regulated by iron and expressed in the phloem[J]. The Plant Journal, 2004, 39(3): 415-424.PMID: 15255870</ref>
<ref name="ref2"> Ishimaru Y, Masuda H, Bashir K, et al. Rice metal‐nicotianamine transporter, OsYSL2, is required for the long‐distance transport of iron and manganese[J]. The Plant Journal, 2010, 62(3): 379-390.PMID: 20128878</ref>
<ref name="ref3"> Nozoye T, Inoue H, Takahashi M, et al. The expression of iron homeostasis-related genes during rice germination[J]. Plant molecular biology, 2007, 64(1-2): 35-47.PMID: 17333504</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0649900|
Description = Similar to Iron-phytosiderophore transporter protein yellow stripe 1|
Version = NM_001054121.2 GI:297599682 GeneID:4330161|
Length = 3302 bp|
Definition = Oryza sativa Japonica Group Os02g0649900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:27058478..27061779|
CDS = 27058478..27059406,27059491..27059605,27059697..27059886,27059990..27060124,27060204..27060301 ,27061115..27061287,27061395..27061779|
GCID = <gbrowseImage1>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</cdnaseq>|
AA = <aaseq>MEAAAPEIERCDAGDVESDHDGAAAAAERVPPWREQVTARGMVA ALLIGFVYTVIIMKLALTTGIIPTLNVSAALLAFLALRGWTRAPALLLPGGGAASSSS RRRPFTRQENTVVQTCAVACYTMGFGGGFGSSLLALNRKTYELAGVSTPGNSPGSYKE PGVGWMTGFLFAISFVGLLNLLPLRKALIIDYKLTYPSGTATAVLINGFHTPQGENSA KKQVRGFLNCFGISLLWSFFQWFYTGGESCGFLQFPTFGLKAWKQTFYFDFSLTYVGA GMICSHLVNLSALFGAILSWGIMWPLISIQKGKWYPGNVPESSMTSLFGYKSFMCVAL IMGDGLYHFIKVTGITAKSLHERSNRRHAKKATDEDTFVIADMQRDEFFNKDYIPNWL AYAGYALLSIVAVIAIPIMFQQVKWYYVVVAFVLAPVLGFSNAYGTGLTDMNMSYNYG KIALFIFAAWGGRDNGVIAGLVGCGIVKQLVQVSADLMHDFKTGHLTLTSPRSMLVGQ AIGTAMGCIIAPLTFLLFYKAFDIGNPDGYWKAPYALIFRNMAILGVEGFSALPKHCL ELSAGFFAFSVLINLMRDFLPRKYRDYVPLPTAMAVPFLVGANFAIDMCVGSLIVFAW HKINSKESALLVPAVASGFICGDGIWMFPSSLLSLAKVKPPICMKFTPGS</aaseq>|
DNA = <dnaseqindica>2374..3302#2175..2289#1894..2083#1656..1790#1479..1576#493..665#1..385#atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgcgcgccccgctactacttcctgtttgatttctctccccaagtctttcttaatttgttcttgcgatggcgcctgtatataaaattgggggatatcgtgcgcgcaggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggtgtgtactttgcccacaatttttgttccgttggtactgaatatgcaatggattaaattagacctgtccctgcaaattcaggactctctggagttcctgctattatgttcgttaattgttgtgatttgctatctttcagtgaactgtttttttctctcagttttgtttcggatttttggagaatatagctcagttgttgcatgtgaaatctgtgctatattctcagttttgtgctgatattaaaaattgtgaactcactcctattttaagatgatattttacataaacaatctgttgttacatttgtaaatttgtctgatcccagtatacggataggtcatgttttcaagaaagactaggaagtttaagttacccatcagtctaccactatctgttttactttgttaggttcttgatctgctttatcttgctaacctttaaggctttaactagaactacttgttcagactccagagtaatgcacatcgtgtgcatggcatggaaacccaactaacctgcaacagttcaacaccaattgggagttctacatagtacacatcctaatatttggtatgcggcattacatttccattatccttaaccaacaagttgactaaagtgctaaaacgaaatgaacttttttgagacaaataaaatgtcatagactaaatgagacaactgtacacacgaattccctctagatactcttcttcaagtctaaaaaatttgtcattacaatctgtagtttgaagacagaaaccagttttctttgcatttgtctgtcccacctaatgatgtgcatgacgttttcaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagtatggtccaggtctcacttgtgctaaatacatcatcgcagttcgtgatatgaattattaattctacttttcatgccaggaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgtatgcattattggtcacccattgtttgtacccctactttatgcataattgcatattcgtcaggcagccagattctcctaactaccaattttttttgctgcaggttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaaggtcaggcatacaatttacctttttctcaaaatagtctgatgtgaatccataattttatatggtacagtatctctaattttctccttctcagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaaggtaaacgtgctctgcgaaattaactatttccacgttttcttccctcttatatcaccaaggtagctgaagctgagtgaattgcagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054121.2 RefSeq:Os02g0649900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0649900

2014-05-19T09:41:39Z

Eric Fu: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsYSL2 is an Fe-regulated metal-NA transporter that is involved in phloem transport and the translocation of mineral nutrients in grains<ref name="ref1" />. The altered expression of OsYSL2changes the localization of Fe, and that OsYSL2 is a critical Fe-nicotianamine transporter important for Fe translocation,especially in the shoots and endosperm <ref name="ref2" />.Based on the nucleotide sequence,OsYSL2 was predicted to encode a polypeptide of 674 amino acids containing 14 putative transmembrane domains(figure 1a)<ref name="ref1" />. Cells expressing the OsYSL2–GFP fusion protein showed a signal in the plasma membrane, with the presence of the putative transmembrane domains shows that OsYSL2 is a transporter that is localized in the plasma membrane(figure 1b)<ref name="ref1" />.

===Expression===
OsYSL2 was of particular interest because no detectable transcripts were present in the roots of either Fe-sufficient or Fe-deficient plants, but dramatic expression was induced in the leaves of Fe-deficient plants<ref name="ref1" />.Research by Yasuhiro Ishimaru et al. showed that the high expression of OsYSL2 in the roots under Fe deficiency was not consistent with previous study<ref name="ref2" />. The cell-type specificity of OsYSL2 expression was investigated using promoter (1.5 kb):b-glucuronidase (GUS) analysis.GUS staining was observed in phloem cells of the vascular bundles of leaves and leaf sheaths of Fe-sufficient rice(Figure 3e–g). The phloem-specific expression of the OsYSL2 promoter suggested that OsYSL2 is involved in the phloem transport of Fe <ref name="ref1" />. OsYSL2 is expressed during reproductive development in rice. RT-PCR analysis confirmed that OsYSL2 transcripts were increased during early seed development<ref name="ref1" />.
In fully mature seeds,strong OsYSL2 expression was observed in the epithelium, the vascular bundle of the scutellum, and the leaf primordium . Two days after sowing, the OsYSL2 expression level increased in the scutellum near the endosperm. Three days after sowing,OsYSL2 expression was observed in the bran and coleoptile<ref name="ref3" />.

===Evolution===
YS1-like genes in rice Our search for YS1homologues in the Oryza sativaL. Ssp. japonica (cv. Nipponbare) rice genomic database identified
18 putative OsYSL that exhibited 36–76% sequence similarity to YS1 (Figure 4)<ref name="ref1" />.

==Labs working on this gene==
*Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
*Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Kawaguchi 332-0012, Japan
*National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0649900|
Description = Similar to Iron-phytosiderophore transporter protein yellow stripe 1|
Version = NM_001054121.2 GI:297599682 GeneID:4330161|
Length = 3302 bp|
Definition = Oryza sativa Japonica Group Os02g0649900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:27058478..27061779|
CDS = 27058478..27059406,27059491..27059605,27059697..27059886,27059990..27060124,27060204..27060301 ,27061115..27061287,27061395..27061779|
GCID = <gbrowseImage1>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</cdnaseq>|
AA = <aaseq>MEAAAPEIERCDAGDVESDHDGAAAAAERVPPWREQVTARGMVA ALLIGFVYTVIIMKLALTTGIIPTLNVSAALLAFLALRGWTRAPALLLPGGGAASSSS RRRPFTRQENTVVQTCAVACYTMGFGGGFGSSLLALNRKTYELAGVSTPGNSPGSYKE PGVGWMTGFLFAISFVGLLNLLPLRKALIIDYKLTYPSGTATAVLINGFHTPQGENSA KKQVRGFLNCFGISLLWSFFQWFYTGGESCGFLQFPTFGLKAWKQTFYFDFSLTYVGA GMICSHLVNLSALFGAILSWGIMWPLISIQKGKWYPGNVPESSMTSLFGYKSFMCVAL IMGDGLYHFIKVTGITAKSLHERSNRRHAKKATDEDTFVIADMQRDEFFNKDYIPNWL AYAGYALLSIVAVIAIPIMFQQVKWYYVVVAFVLAPVLGFSNAYGTGLTDMNMSYNYG KIALFIFAAWGGRDNGVIAGLVGCGIVKQLVQVSADLMHDFKTGHLTLTSPRSMLVGQ AIGTAMGCIIAPLTFLLFYKAFDIGNPDGYWKAPYALIFRNMAILGVEGFSALPKHCL ELSAGFFAFSVLINLMRDFLPRKYRDYVPLPTAMAVPFLVGANFAIDMCVGSLIVFAW HKINSKESALLVPAVASGFICGDGIWMFPSSLLSLAKVKPPICMKFTPGS</aaseq>|
DNA = <dnaseqindica>2374..3302#2175..2289#1894..2083#1656..1790#1479..1576#493..665#1..385#atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgcgcgccccgctactacttcctgtttgatttctctccccaagtctttcttaatttgttcttgcgatggcgcctgtatataaaattgggggatatcgtgcgcgcaggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggtgtgtactttgcccacaatttttgttccgttggtactgaatatgcaatggattaaattagacctgtccctgcaaattcaggactctctggagttcctgctattatgttcgttaattgttgtgatttgctatctttcagtgaactgtttttttctctcagttttgtttcggatttttggagaatatagctcagttgttgcatgtgaaatctgtgctatattctcagttttgtgctgatattaaaaattgtgaactcactcctattttaagatgatattttacataaacaatctgttgttacatttgtaaatttgtctgatcccagtatacggataggtcatgttttcaagaaagactaggaagtttaagttacccatcagtctaccactatctgttttactttgttaggttcttgatctgctttatcttgctaacctttaaggctttaactagaactacttgttcagactccagagtaatgcacatcgtgtgcatggcatggaaacccaactaacctgcaacagttcaacaccaattgggagttctacatagtacacatcctaatatttggtatgcggcattacatttccattatccttaaccaacaagttgactaaagtgctaaaacgaaatgaacttttttgagacaaataaaatgtcatagactaaatgagacaactgtacacacgaattccctctagatactcttcttcaagtctaaaaaatttgtcattacaatctgtagtttgaagacagaaaccagttttctttgcatttgtctgtcccacctaatgatgtgcatgacgttttcaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagtatggtccaggtctcacttgtgctaaatacatcatcgcagttcgtgatatgaattattaattctacttttcatgccaggaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgtatgcattattggtcacccattgtttgtacccctactttatgcataattgcatattcgtcaggcagccagattctcctaactaccaattttttttgctgcaggttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaaggtcaggcatacaatttacctttttctcaaaatagtctgatgtgaatccataattttatatggtacagtatctctaattttctccttctcagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaaggtaaacgtgctctgcgaaattaactatttccacgttttcttccctcttatatcaccaaggtagctgaagctgagtgaattgcagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054121.2 RefSeq:Os02g0649900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0649900

2014-05-19T09:40:38Z

Eric Fu: /* Evolution */

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsYSL2 is an Fe-regulated metal-NA transporter that is involved in phloem transport and the translocation of mineral nutrients in grains<ref name="ref1" />. The altered expression of OsYSL2changes the localization of Fe, and that OsYSL2 is a critical Fe-nicotianamine transporter important for Fe translocation,especially in the shoots and endosperm <ref name="ref2" />.Based on the nucleotide sequence,OsYSL2 was predicted to encode a polypeptide of 674 amino acids containing 14 putative transmembrane domains(figure 1a)<ref name="ref1" />. Cells expressing the OsYSL2–GFP fusion protein showed a signal in the plasma membrane, with the presence of the putative transmembrane domains shows that OsYSL2 is a transporter that is localized in the plasma membrane(figure 1b)<ref name="ref1" />.

===Expression===
OsYSL2 was of particular interest because no detectable transcripts were present in the roots of either Fe-sufficient or Fe-deficient plants, but dramatic expression was induced in the leaves of Fe-deficient plants<ref name="ref1" />.Research by Yasuhiro Ishimaru et al. showed that the high expression of OsYSL2 in the roots under Fe deficiency was not consistent with previous study<ref name="ref2" />. The cell-type specificity of OsYSL2 expression was investigated using promoter (1.5 kb):b-glucuronidase (GUS) analysis.GUS staining was observed in phloem cells of the vascular bundles of leaves and leaf sheaths of Fe-sufficient rice(Figure 3e–g). The phloem-specific expression of the OsYSL2 promoter suggested that OsYSL2 is involved in the phloem transport of Fe <ref name="ref1" />. OsYSL2 is expressed during reproductive development in rice. RT-PCR analysis confirmed that OsYSL2 transcripts were increased during early seed development<ref name="ref1" />.
In fully mature seeds,strong OsYSL2 expression was observed in the epithelium, the vascular bundle of the scutellum, and the leaf primordium . Two days after sowing, the OsYSL2 expression level increased in the scutellum near the endosperm. Three days after sowing,OsYSL2 expression was observed in the bran and coleoptile<ref name="ref3" />.

===Evolution===
YS1-like genes in rice Our search for YS1homologues in the Oryza sativaL. Ssp. japonica (cv. Nipponbare) rice genomic database identified
18 putative OsYSL that exhibited 36–76% sequence similarity to YS1 (Figure 4)<ref name="ref1" />.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0649900|
Description = Similar to Iron-phytosiderophore transporter protein yellow stripe 1|
Version = NM_001054121.2 GI:297599682 GeneID:4330161|
Length = 3302 bp|
Definition = Oryza sativa Japonica Group Os02g0649900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:27058478..27061779|
CDS = 27058478..27059406,27059491..27059605,27059697..27059886,27059990..27060124,27060204..27060301 ,27061115..27061287,27061395..27061779|
GCID = <gbrowseImage1>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</cdnaseq>|
AA = <aaseq>MEAAAPEIERCDAGDVESDHDGAAAAAERVPPWREQVTARGMVA ALLIGFVYTVIIMKLALTTGIIPTLNVSAALLAFLALRGWTRAPALLLPGGGAASSSS RRRPFTRQENTVVQTCAVACYTMGFGGGFGSSLLALNRKTYELAGVSTPGNSPGSYKE PGVGWMTGFLFAISFVGLLNLLPLRKALIIDYKLTYPSGTATAVLINGFHTPQGENSA KKQVRGFLNCFGISLLWSFFQWFYTGGESCGFLQFPTFGLKAWKQTFYFDFSLTYVGA GMICSHLVNLSALFGAILSWGIMWPLISIQKGKWYPGNVPESSMTSLFGYKSFMCVAL IMGDGLYHFIKVTGITAKSLHERSNRRHAKKATDEDTFVIADMQRDEFFNKDYIPNWL AYAGYALLSIVAVIAIPIMFQQVKWYYVVVAFVLAPVLGFSNAYGTGLTDMNMSYNYG KIALFIFAAWGGRDNGVIAGLVGCGIVKQLVQVSADLMHDFKTGHLTLTSPRSMLVGQ AIGTAMGCIIAPLTFLLFYKAFDIGNPDGYWKAPYALIFRNMAILGVEGFSALPKHCL ELSAGFFAFSVLINLMRDFLPRKYRDYVPLPTAMAVPFLVGANFAIDMCVGSLIVFAW HKINSKESALLVPAVASGFICGDGIWMFPSSLLSLAKVKPPICMKFTPGS</aaseq>|
DNA = <dnaseqindica>2374..3302#2175..2289#1894..2083#1656..1790#1479..1576#493..665#1..385#atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgcgcgccccgctactacttcctgtttgatttctctccccaagtctttcttaatttgttcttgcgatggcgcctgtatataaaattgggggatatcgtgcgcgcaggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggtgtgtactttgcccacaatttttgttccgttggtactgaatatgcaatggattaaattagacctgtccctgcaaattcaggactctctggagttcctgctattatgttcgttaattgttgtgatttgctatctttcagtgaactgtttttttctctcagttttgtttcggatttttggagaatatagctcagttgttgcatgtgaaatctgtgctatattctcagttttgtgctgatattaaaaattgtgaactcactcctattttaagatgatattttacataaacaatctgttgttacatttgtaaatttgtctgatcccagtatacggataggtcatgttttcaagaaagactaggaagtttaagttacccatcagtctaccactatctgttttactttgttaggttcttgatctgctttatcttgctaacctttaaggctttaactagaactacttgttcagactccagagtaatgcacatcgtgtgcatggcatggaaacccaactaacctgcaacagttcaacaccaattgggagttctacatagtacacatcctaatatttggtatgcggcattacatttccattatccttaaccaacaagttgactaaagtgctaaaacgaaatgaacttttttgagacaaataaaatgtcatagactaaatgagacaactgtacacacgaattccctctagatactcttcttcaagtctaaaaaatttgtcattacaatctgtagtttgaagacagaaaccagttttctttgcatttgtctgtcccacctaatgatgtgcatgacgttttcaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagtatggtccaggtctcacttgtgctaaatacatcatcgcagttcgtgatatgaattattaattctacttttcatgccaggaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgtatgcattattggtcacccattgtttgtacccctactttatgcataattgcatattcgtcaggcagccagattctcctaactaccaattttttttgctgcaggttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaaggtcaggcatacaatttacctttttctcaaaatagtctgatgtgaatccataattttatatggtacagtatctctaattttctccttctcagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaaggtaaacgtgctctgcgaaattaactatttccacgttttcttccctcttatatcaccaaggtagctgaagctgagtgaattgcagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054121.2 RefSeq:Os02g0649900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0649900

2014-05-19T09:38:24Z

Eric Fu: /* Expression */

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsYSL2 is an Fe-regulated metal-NA transporter that is involved in phloem transport and the translocation of mineral nutrients in grains<ref name="ref1" />. The altered expression of OsYSL2changes the localization of Fe, and that OsYSL2 is a critical Fe-nicotianamine transporter important for Fe translocation,especially in the shoots and endosperm <ref name="ref2" />.Based on the nucleotide sequence,OsYSL2 was predicted to encode a polypeptide of 674 amino acids containing 14 putative transmembrane domains(figure 1a)<ref name="ref1" />. Cells expressing the OsYSL2–GFP fusion protein showed a signal in the plasma membrane, with the presence of the putative transmembrane domains shows that OsYSL2 is a transporter that is localized in the plasma membrane(figure 1b)<ref name="ref1" />.

===Expression===
OsYSL2 was of particular interest because no detectable transcripts were present in the roots of either Fe-sufficient or Fe-deficient plants, but dramatic expression was induced in the leaves of Fe-deficient plants<ref name="ref1" />.Research by Yasuhiro Ishimaru et al. showed that the high expression of OsYSL2 in the roots under Fe deficiency was not consistent with previous study<ref name="ref2" />. The cell-type specificity of OsYSL2 expression was investigated using promoter (1.5 kb):b-glucuronidase (GUS) analysis.GUS staining was observed in phloem cells of the vascular bundles of leaves and leaf sheaths of Fe-sufficient rice(Figure 3e–g). The phloem-specific expression of the OsYSL2 promoter suggested that OsYSL2 is involved in the phloem transport of Fe <ref name="ref1" />. OsYSL2 is expressed during reproductive development in rice. RT-PCR analysis confirmed that OsYSL2 transcripts were increased during early seed development<ref name="ref1" />.
In fully mature seeds,strong OsYSL2 expression was observed in the epithelium, the vascular bundle of the scutellum, and the leaf primordium . Two days after sowing, the OsYSL2 expression level increased in the scutellum near the endosperm. Three days after sowing,OsYSL2 expression was observed in the bran and coleoptile<ref name="ref3" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0649900|
Description = Similar to Iron-phytosiderophore transporter protein yellow stripe 1|
Version = NM_001054121.2 GI:297599682 GeneID:4330161|
Length = 3302 bp|
Definition = Oryza sativa Japonica Group Os02g0649900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:27058478..27061779|
CDS = 27058478..27059406,27059491..27059605,27059697..27059886,27059990..27060124,27060204..27060301 ,27061115..27061287,27061395..27061779|
GCID = <gbrowseImage1>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</cdnaseq>|
AA = <aaseq>MEAAAPEIERCDAGDVESDHDGAAAAAERVPPWREQVTARGMVA ALLIGFVYTVIIMKLALTTGIIPTLNVSAALLAFLALRGWTRAPALLLPGGGAASSSS RRRPFTRQENTVVQTCAVACYTMGFGGGFGSSLLALNRKTYELAGVSTPGNSPGSYKE PGVGWMTGFLFAISFVGLLNLLPLRKALIIDYKLTYPSGTATAVLINGFHTPQGENSA KKQVRGFLNCFGISLLWSFFQWFYTGGESCGFLQFPTFGLKAWKQTFYFDFSLTYVGA GMICSHLVNLSALFGAILSWGIMWPLISIQKGKWYPGNVPESSMTSLFGYKSFMCVAL IMGDGLYHFIKVTGITAKSLHERSNRRHAKKATDEDTFVIADMQRDEFFNKDYIPNWL AYAGYALLSIVAVIAIPIMFQQVKWYYVVVAFVLAPVLGFSNAYGTGLTDMNMSYNYG KIALFIFAAWGGRDNGVIAGLVGCGIVKQLVQVSADLMHDFKTGHLTLTSPRSMLVGQ AIGTAMGCIIAPLTFLLFYKAFDIGNPDGYWKAPYALIFRNMAILGVEGFSALPKHCL ELSAGFFAFSVLINLMRDFLPRKYRDYVPLPTAMAVPFLVGANFAIDMCVGSLIVFAW HKINSKESALLVPAVASGFICGDGIWMFPSSLLSLAKVKPPICMKFTPGS</aaseq>|
DNA = <dnaseqindica>2374..3302#2175..2289#1894..2083#1656..1790#1479..1576#493..665#1..385#atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgcgcgccccgctactacttcctgtttgatttctctccccaagtctttcttaatttgttcttgcgatggcgcctgtatataaaattgggggatatcgtgcgcgcaggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggtgtgtactttgcccacaatttttgttccgttggtactgaatatgcaatggattaaattagacctgtccctgcaaattcaggactctctggagttcctgctattatgttcgttaattgttgtgatttgctatctttcagtgaactgtttttttctctcagttttgtttcggatttttggagaatatagctcagttgttgcatgtgaaatctgtgctatattctcagttttgtgctgatattaaaaattgtgaactcactcctattttaagatgatattttacataaacaatctgttgttacatttgtaaatttgtctgatcccagtatacggataggtcatgttttcaagaaagactaggaagtttaagttacccatcagtctaccactatctgttttactttgttaggttcttgatctgctttatcttgctaacctttaaggctttaactagaactacttgttcagactccagagtaatgcacatcgtgtgcatggcatggaaacccaactaacctgcaacagttcaacaccaattgggagttctacatagtacacatcctaatatttggtatgcggcattacatttccattatccttaaccaacaagttgactaaagtgctaaaacgaaatgaacttttttgagacaaataaaatgtcatagactaaatgagacaactgtacacacgaattccctctagatactcttcttcaagtctaaaaaatttgtcattacaatctgtagtttgaagacagaaaccagttttctttgcatttgtctgtcccacctaatgatgtgcatgacgttttcaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagtatggtccaggtctcacttgtgctaaatacatcatcgcagttcgtgatatgaattattaattctacttttcatgccaggaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgtatgcattattggtcacccattgtttgtacccctactttatgcataattgcatattcgtcaggcagccagattctcctaactaccaattttttttgctgcaggttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaaggtcaggcatacaatttacctttttctcaaaatagtctgatgtgaatccataattttatatggtacagtatctctaattttctccttctcagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaaggtaaacgtgctctgcgaaattaactatttccacgttttcttccctcttatatcaccaaggtagctgaagctgagtgaattgcagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054121.2 RefSeq:Os02g0649900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0649900

2014-05-19T09:34:22Z

Eric Fu: /* Function */

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsYSL2 is an Fe-regulated metal-NA transporter that is involved in phloem transport and the translocation of mineral nutrients in grains<ref name="ref1" />. The altered expression of OsYSL2changes the localization of Fe, and that OsYSL2 is a critical Fe-nicotianamine transporter important for Fe translocation,especially in the shoots and endosperm <ref name="ref2" />.Based on the nucleotide sequence,OsYSL2 was predicted to encode a polypeptide of 674 amino acids containing 14 putative transmembrane domains(figure 1a)<ref name="ref1" />. Cells expressing the OsYSL2–GFP fusion protein showed a signal in the plasma membrane, with the presence of the putative transmembrane domains shows that OsYSL2 is a transporter that is localized in the plasma membrane(figure 1b)<ref name="ref1" />.

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0649900|
Description = Similar to Iron-phytosiderophore transporter protein yellow stripe 1|
Version = NM_001054121.2 GI:297599682 GeneID:4330161|
Length = 3302 bp|
Definition = Oryza sativa Japonica Group Os02g0649900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:27058478..27061779|
CDS = 27058478..27059406,27059491..27059605,27059697..27059886,27059990..27060124,27060204..27060301 ,27061115..27061287,27061395..27061779|
GCID = <gbrowseImage1>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:27058478..27061779
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</cdnaseq>|
AA = <aaseq>MEAAAPEIERCDAGDVESDHDGAAAAAERVPPWREQVTARGMVA ALLIGFVYTVIIMKLALTTGIIPTLNVSAALLAFLALRGWTRAPALLLPGGGAASSSS RRRPFTRQENTVVQTCAVACYTMGFGGGFGSSLLALNRKTYELAGVSTPGNSPGSYKE PGVGWMTGFLFAISFVGLLNLLPLRKALIIDYKLTYPSGTATAVLINGFHTPQGENSA KKQVRGFLNCFGISLLWSFFQWFYTGGESCGFLQFPTFGLKAWKQTFYFDFSLTYVGA GMICSHLVNLSALFGAILSWGIMWPLISIQKGKWYPGNVPESSMTSLFGYKSFMCVAL IMGDGLYHFIKVTGITAKSLHERSNRRHAKKATDEDTFVIADMQRDEFFNKDYIPNWL AYAGYALLSIVAVIAIPIMFQQVKWYYVVVAFVLAPVLGFSNAYGTGLTDMNMSYNYG KIALFIFAAWGGRDNGVIAGLVGCGIVKQLVQVSADLMHDFKTGHLTLTSPRSMLVGQ AIGTAMGCIIAPLTFLLFYKAFDIGNPDGYWKAPYALIFRNMAILGVEGFSALPKHCL ELSAGFFAFSVLINLMRDFLPRKYRDYVPLPTAMAVPFLVGANFAIDMCVGSLIVFAW HKINSKESALLVPAVASGFICGDGIWMFPSSLLSLAKVKPPICMKFTPGS</aaseq>|
DNA = <dnaseqindica>2374..3302#2175..2289#1894..2083#1656..1790#1479..1576#493..665#1..385#atggaagccgccgctcccgagatagagaggtgcgacgccggcgacgtggagtcggaccacgacggcgccgccgccgccgccgagcgcgtgccgccgtggcgcgagcaggtgacggcgcggggcatggtggcggcgctgctcatcggattcgtctacaccgtcatcatcatgaagctggcgctcaccacggggatcatccccacgctcaacgtctccgccgcgctcctcgccttcctcgcgctccgcggctggacgcgcgcgcccgccctcctcctccccggcggcggcgccgcctcctcctcctcccggcgccgccccttcacccgccaggagaacaccgttgtccagacctgcgccgtcgcctgctacaccatgggattcggcggtgcgcgccccgctactacttcctgtttgatttctctccccaagtctttcttaatttgttcttgcgatggcgcctgtatataaaattgggggatatcgtgcgcgcaggtgggttcgggtcgtcgctgctggctctgaacaggaagacgtacgagttggccggggtgagcacgccgggcaactcgccggggagctacaaggagcccggggtcgggtggatgaccggattcctgtttgcgattagcttcgttgggctccttaacttgcttccactcagaaaggtgtgtactttgcccacaatttttgttccgttggtactgaatatgcaatggattaaattagacctgtccctgcaaattcaggactctctggagttcctgctattatgttcgttaattgttgtgatttgctatctttcagtgaactgtttttttctctcagttttgtttcggatttttggagaatatagctcagttgttgcatgtgaaatctgtgctatattctcagttttgtgctgatattaaaaattgtgaactcactcctattttaagatgatattttacataaacaatctgttgttacatttgtaaatttgtctgatcccagtatacggataggtcatgttttcaagaaagactaggaagtttaagttacccatcagtctaccactatctgttttactttgttaggttcttgatctgctttatcttgctaacctttaaggctttaactagaactacttgttcagactccagagtaatgcacatcgtgtgcatggcatggaaacccaactaacctgcaacagttcaacaccaattgggagttctacatagtacacatcctaatatttggtatgcggcattacatttccattatccttaaccaacaagttgactaaagtgctaaaacgaaatgaacttttttgagacaaataaaatgtcatagactaaatgagacaactgtacacacgaattccctctagatactcttcttcaagtctaaaaaatttgtcattacaatctgtagtttgaagacagaaaccagttttctttgcatttgtctgtcccacctaatgatgtgcatgacgttttcaggctttgatcatagattataagttaacctatccaagtgggactgcaactgctgttcttataaacggattccatacccctcaaggagaaaatagtgcaaagtatggtccaggtctcacttgtgctaaatacatcatcgcagttcgtgatatgaattattaattctacttttcatgccaggaagcaagtccgcggatttctgaattgctttgggatcagcctcttatggagcttcttccagtggttctacactggtggagagagttgtgggtttcttcaattccctacctttggtctaaaggcttggaaacaaacgtatgcattattggtcacccattgtttgtacccctactttatgcataattgcatattcgtcaggcagccagattctcctaactaccaattttttttgctgcaggttctattttgatttcagcctcacatatgttggtgcgggaatgatctgctcacaccttgtaaacctctccgcgctctttggcgcaatactttcttgggggataatgtggccactgatcagcatacaaaagggtaagtggtatcctggaaatgttcctgaaagcagcatgacaagcttgtttggttacaaggtcaggcatacaatttacctttttctcaaaatagtctgatgtgaatccataattttatatggtacagtatctctaattttctccttctcagtccttcatgtgtgtagctttgatcatgggggatggcctttaccacttcataaaagtaacaggtatcactgctaagagcctgcatgaacgatccaatcgtagacatgctaagaaaggtaaacgtgctctgcgaaattaactatttccacgttttcttccctcttatatcaccaaggtagctgaagctgagtgaattgcagcgacagatgaggatacatttgtaattgctgatatgcagcgtgacgagttcttcaacaaagactatattccaaactggctagcgtatgctggatatgccttactgagcattgttgcggtaattgcgatacccataatgttccaacaggtgaagtggtactatgttgttgtagcctttgtgcttgctcctgtgctgggattctccaatgcctatggaactgggctaactgacatgaacatgagctacaactatggcaagattgctctcttcatctttgcagcttggggagggagggacaacggtgtcattgctggtcttgttggttgtggaatagtgaagcagctagtacaagtctctgctgatttgatgcacgactttaagactggtcatctgacattaacatcaccaagatccatgcttgttgggcaggccattggtacagccatgggctgcataatcgctccactgactttcttgctcttctacaaagcatttgatattgggaaccctgatggatactggaaggctccatatgccctgatatttcgaaacatggccattcttggcgtcgagggcttttctgcactaccaaagcattgtcttgagctgtctgctggcttctttgcattttctgtgctcatcaaccttatgagggacttcttgccacgcaagtacagggattatgtgcccctgccgacggcaatggctgtgccattccttgttggtgcaaattttgctattgacatgtgtgtgggaagcctgatagtctttgcctggcacaagatcaacagcaaggagtctgcgttgctagtgccagcggttgcgtctggttttatttgtggagatggtatatggatgttcccttcttccctgctttcccttgctaaggttaagccaccaatctgcatgaagttcactcccggaagctag</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054121.2 RefSeq:Os02g0649900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]