http://192.168.164.12:81/ricewiki/api.php?action=feedcontributions&feedformat=atom&user=Fangchao 2026-05-26T03:11:39Z User contributions MediaWiki 1.30.0 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0122600&diff=171670 2014-05-24T07:12:13Z

<p>Fangchao: </p> <hr /> <div>OsMADS50 functions as a LD-specific flowering activator.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> [[File:Molecular network.jpg|right|thumb|150px|''Molecular network (from reference &lt;ref name=&quot;ref1&quot; /&gt;).'']]<br /> <br /> [[File:Schematic diagram of OsMADS50 genomic structure, positions of T-DNA insertional mutant alleles and flowering time of mutants.gif|right|thumb|150px|''Schematic diagram of OsMADS50 genomic structure &lt;ref name=&quot;ref3&quot; /&gt;).'']]<br /> <br /> OsMADS50 functions as a LD-specific flowering activator that functions only under LD conditions&lt;ref name=&quot;ref3&quot; /&gt;.<br /> <br /> OsMADS50 encodes MIKC-type MADS-box proteins . MADS-box genes play important roles in several developmental processes, e.g. control of flowering time, floral organ identity and fruit developmen.<br /> <br /> OsMADS50 serves as a flowering activator by controlling various floral regulators such as OsMADS1, OsMADS14, OsMADS15, OsMADS18 and Hd3a.It causing early flowering when overexpressed and delayed flowering when suppressed.OsMADS50 activates Ehd1 expression, and in turn Ehd1 activates RFT1 expression. RFT1 acts as the LD-specific florigen(from reference &lt;ref name=&quot;ref1&quot; /&gt;).<br /> <br /> <br /> ===Mutation===<br /> [[File:Phenotype comparison between OsMADS50 KO and WT plants.gif|right|thumb|200px|''Phenotype comparison between OsMADS50 KO and WT plants '']]<br /> <br /> OsMADS50 KO plants&lt;ref name=&quot;ref2&quot; /&gt;<br /> <br /> <br /> ===Expression===<br /> [[File:Expression.gif|right|thumb|150px|'' Expression profiles of OsMADS50 and other flowering regulators. (from reference &lt;ref name=&quot;ref2&quot; /&gt;).'']]<br /> <br /> RNA gel blot analysis revealed that OsMADS50 was variably expressed in most organs (Figure 6a). This gene was detected at a low level during the seedling stage, with transcripts increasing as the plant matured. In young panicles, expression was initially low, and continued to decline as these organs matured. RT-PCR analysis of leaves at four developmental stages confirmed the RNA gel blot analysis (Figure 6b). OsMADS50 transcript was detected at all four stages, with the expression level slightly increasing in 49-day-old plants compared with those that were 20 days old. The transcripts of Hd3a, OsMADS14, OsMADS15, and OsMADS18 increased gradually, reaching a maximum at 80 days&lt;ref name=&quot;ref2&quot; /&gt;.<br /> <br /> <br /> ===Evolution===<br /> [[File:Dendrigram.jpg|right|thumb|150px|'Dendrigram .'']]<br /> <br /> In rice, OsMADS50 is the protein most homologous with SOC1/AGL20, showing 50.6% identity, whereas OsMADS56 has 48.3% identity. Genome-wide phylogenic analysis showed that OsMADS50 is clustered with the SOC1-group MADS-box proteins.&lt;ref name=&quot;ref4&quot; /&gt;<br /> <br /> <br /> ===Knowledge Extension===<br /> [[File:PCE 2008 f8.gif|right|thumb|150px|'' Interaction analyses between OsMADS50 and OsMADS56&lt;ref name=&quot;ref2&quot; /&gt;.'']]<br /> <br /> OsMADS50 is the putative ortholog of SOC1/AGL20.<br /> <br /> OsMADS50 is upstream of OsMADS1, OsMADS14, OsMADS15, and OsMADS18.Expression analyses of the MADS-box genes in WT plants demonstrated that <br /> <br /> OsMADS50 acts upstream of the other MADS-box genes.<br /> <br /> OsMADS50 physically interacts with OsMADS56.Because both OsMADS50 and OsMADS56 appear to function as LD-specific regulators,the OsMADS50 KC region indeed binds to the OsMADS56 KC.&lt;ref name=&quot;ref3&quot; /&gt;<br /> <br /> OsMADS50 is epistatic to Hd3a.Three genes, OsGI, Hd1, and Hd3a, play major roles in the photoperiodic flowering pathway.The expression of OsGI and Hd1 was not affected by OsMADS50, thereby indicating that they act upstream of or in parallel with OsMADS50. In contrast, Hd3a was downregulated in the OsMADS50 KO plants, implying that OsMADS50 is epistatic to Hd3a&lt;ref name=&quot;ref2&quot; /&gt;.<br /> <br /> <br /> ==Labs working on this gene==<br /> Department of Life Science and Functional Genomic Center, Pohang University of Science and Technology (POSTECH)<br /> <br /> National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center,Nanjing Agricultural University<br /> <br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;STsuji H, Taoka K, Shimamoto K. Regulation of flowering in rice: two florigen genes, a complex gene network, and natural variation[J]. Current opinion in plant biology, 2011, 14(1): 45-52.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Lee S, Kim J, Han J J, et al. Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS‐LIKE 20 (SOC1/AGL20) ortholog in rice[J]. The Plant Journal, 2004, 38(5): 754-764.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref3&quot;&gt;RYU C H, Lee S, CHO L A E H, et al. OsMADS50 and OsMADS56 function antagonistically in regulating long day (LD)‐dependent flowering in rice[J]. Plant, cell &amp; environment, 2009, 32(10): 1412-1427.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref4&quot;&gt;Lee S Y, Jang S H, Jun S H, et al. Functional analysis of MADS-box genes expressed preferentially in vegetative tissues[C]//Advances in rice genetics, Los Baños, Laguna, Philippines, 22-27 October 2000. World Scientific Publishing Co. Pte. Ltd, 2003: 414-416.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0122600|<br /> Description = Transcription factor, MADS-box domain containing protein|<br /> Version = NM_001055336.1 GI:115450400 GeneID:4331445|<br /> Length = 29946 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0122600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:1248675..1278620|<br /> CDS = 1248915..1249100,1249297..1249338,1249458..1249499,1249599..1249698,1249789..1249850&lt;br&gt;,1250412..1250490,1278122..1278303|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:1248675..1278620<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:1248675..1278620<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggtgcgggggaagacgcagatgaagcggatagagaaccccacgagccgccaggtcaccttctccaagcgccgcaacggcctgctcaagaaggccttcgagctctccgtcctctgcgacgccgaggtcgcgctcatcgtcttctccccgcgcggcaagctctacgaattcgccagcgccagtacgcagaaaacaattgaacgctataggacgtatacaaaggaaaatatcggcaacaagacagtacagcaagatatagagcaagtaaaagctgacgctgatggtttggcaaagaaacttgaagctcttgaaacttacaaaagaaaactgctgggtgaaaagttggatgaatgttctattgaagaactgcatagcctggaggtcaagctggagagaagcctcattagcatcaggggaaggaagacaaagctgcttgaggagcaggttgccaaactgagagagaaggagatgaagctgcgcaaggacaatgaagagttacgcgaaaagtgtaagaatcagcctcccttgtctgctcctttgactgtccgggccgaagatgagaacccggaccgtaacatcaacaccaccaacgacaacatggatgtcgaaactgagctattcatagggctgcctggcagaagtcgctccagcggcggtgctgcagaagatagccaagcgatgccccattcttaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MVRGKTQMKRIENPTSRQVTFSKRRNGLLKKAFELSVLCDAEVA LIVFSPRGKLYEFASASTQKTIERYRTYTKENIGNKTVQQDIEQVKADADGLAKKLEA LETYKRKLLGEKLDECSIEELHSLEVKLERSLISIRGRKTKLLEEQVAKLREKEMKLR KDNEELREKCKNQPPLSAPLTVRAEDENPDRNINTTNDNMDVETELFIGLPGRSRSSG GAAEDSQAMPHS&lt;/aaseq&gt;|<br /> DNA = 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cgatcggcagactccactacccatctaaatctaaatcgagagatctcaagtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055336.1 RefSeq:Os03g0122600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Fangchao https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0122600&diff=171668 2014-05-24T07:11:38Z

<p>Fangchao: </p> <hr /> <div>OsMADS50 functions as a LD-specific flowering activator.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> [[File:Molecular network.jpg|right|thumb|150px|''Molecular network (from reference &lt;ref name=&quot;ref1&quot; /&gt;).'']]<br /> <br /> [[File:File:Schematic diagram of OsMADS50 genomic structure, positions of T-DNA insertional mutant alleles and flowering time of mutants.gif|right|thumb|150px|''Schematic diagram of OsMADS50 genomic structure &lt;ref name=&quot;ref3&quot; /&gt;).'']]<br /> <br /> OsMADS50 functions as a LD-specific flowering activator that functions only under LD conditions&lt;ref name=&quot;ref3&quot; /&gt;.<br /> <br /> OsMADS50 encodes MIKC-type MADS-box proteins . MADS-box genes play important roles in several developmental processes, e.g. control of flowering time, floral organ identity and fruit developmen.<br /> <br /> OsMADS50 serves as a flowering activator by controlling various floral regulators such as OsMADS1, OsMADS14, OsMADS15, OsMADS18 and Hd3a.It causing early flowering when overexpressed and delayed flowering when suppressed.OsMADS50 activates Ehd1 expression, and in turn Ehd1 activates RFT1 expression. RFT1 acts as the LD-specific florigen(from reference &lt;ref name=&quot;ref1&quot; /&gt;).<br /> <br /> <br /> ===Mutation===<br /> [[File:Phenotype comparison between OsMADS50 KO and WT plants.gif|right|thumb|200px|''Phenotype comparison between OsMADS50 KO and WT plants '']]<br /> <br /> OsMADS50 KO plants&lt;ref name=&quot;ref2&quot; /&gt;<br /> <br /> <br /> ===Expression===<br /> [[File:Expression.gif|right|thumb|150px|'' Expression profiles of OsMADS50 and other flowering regulators. (from reference &lt;ref name=&quot;ref2&quot; /&gt;).'']]<br /> <br /> RNA gel blot analysis revealed that OsMADS50 was variably expressed in most organs (Figure 6a). This gene was detected at a low level during the seedling stage, with transcripts increasing as the plant matured. In young panicles, expression was initially low, and continued to decline as these organs matured. RT-PCR analysis of leaves at four developmental stages confirmed the RNA gel blot analysis (Figure 6b). OsMADS50 transcript was detected at all four stages, with the expression level slightly increasing in 49-day-old plants compared with those that were 20 days old. The transcripts of Hd3a, OsMADS14, OsMADS15, and OsMADS18 increased gradually, reaching a maximum at 80 days&lt;ref name=&quot;ref2&quot; /&gt;.<br /> <br /> <br /> ===Evolution===<br /> [[File:Dendrigram.jpg|right|thumb|150px|'Dendrigram .'']]<br /> <br /> In rice, OsMADS50 is the protein most homologous with SOC1/AGL20, showing 50.6% identity, whereas OsMADS56 has 48.3% identity. Genome-wide phylogenic analysis showed that OsMADS50 is clustered with the SOC1-group MADS-box proteins.&lt;ref name=&quot;ref4&quot; /&gt;<br /> <br /> <br /> ===Knowledge Extension===<br /> [[File:PCE 2008 f8.gif|right|thumb|150px|'' Interaction analyses between OsMADS50 and OsMADS56&lt;ref name=&quot;ref2&quot; /&gt;.'']]<br /> <br /> OsMADS50 is the putative ortholog of SOC1/AGL20.<br /> <br /> OsMADS50 is upstream of OsMADS1, OsMADS14, OsMADS15, and OsMADS18.Expression analyses of the MADS-box genes in WT plants demonstrated that <br /> <br /> OsMADS50 acts upstream of the other MADS-box genes.<br /> <br /> OsMADS50 physically interacts with OsMADS56.Because both OsMADS50 and OsMADS56 appear to function as LD-specific regulators,the OsMADS50 KC region indeed binds to the OsMADS56 KC.&lt;ref name=&quot;ref3&quot; /&gt;<br /> <br /> OsMADS50 is epistatic to Hd3a.Three genes, OsGI, Hd1, and Hd3a, play major roles in the photoperiodic flowering pathway.The expression of OsGI and Hd1 was not affected by OsMADS50, thereby indicating that they act upstream of or in parallel with OsMADS50. In contrast, Hd3a was downregulated in the OsMADS50 KO plants, implying that OsMADS50 is epistatic to Hd3a&lt;ref name=&quot;ref2&quot; /&gt;.<br /> <br /> <br /> ==Labs working on this gene==<br /> Department of Life Science and Functional Genomic Center, Pohang University of Science and Technology (POSTECH)<br /> <br /> National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center,Nanjing Agricultural University<br /> <br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;STsuji H, Taoka K, Shimamoto K. Regulation of flowering in rice: two florigen genes, a complex gene network, and natural variation[J]. Current opinion in plant biology, 2011, 14(1): 45-52.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Lee S, Kim J, Han J J, et al. Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS‐LIKE 20 (SOC1/AGL20) ortholog in rice[J]. The Plant Journal, 2004, 38(5): 754-764.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref3&quot;&gt;RYU C H, Lee S, CHO L A E H, et al. OsMADS50 and OsMADS56 function antagonistically in regulating long day (LD)‐dependent flowering in rice[J]. Plant, cell &amp; environment, 2009, 32(10): 1412-1427.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref4&quot;&gt;Lee S Y, Jang S H, Jun S H, et al. Functional analysis of MADS-box genes expressed preferentially in vegetative tissues[C]//Advances in rice genetics, Los Baños, Laguna, Philippines, 22-27 October 2000. World Scientific Publishing Co. Pte. Ltd, 2003: 414-416.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0122600|<br /> Description = Transcription factor, MADS-box domain containing protein|<br /> Version = NM_001055336.1 GI:115450400 GeneID:4331445|<br /> Length = 29946 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0122600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:1248675..1278620|<br /> CDS = 1248915..1249100,1249297..1249338,1249458..1249499,1249599..1249698,1249789..1249850&lt;br&gt;,1250412..1250490,1278122..1278303|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:1248675..1278620<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:1248675..1278620<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggtgcgggggaagacgcagatgaagcggatagagaaccccacgagccgccaggtcaccttctccaagcgccgcaacggcctgctcaagaaggccttcgagctctccgtcctctgcgacgccgaggtcgcgctcatcgtcttctccccgcgcggcaagctctacgaattcgccagcgccagtacgcagaaaacaattgaacgctataggacgtatacaaaggaaaatatcggcaacaagacagtacagcaagatatagagcaagtaaaagctgacgctgatggtttggcaaagaaacttgaagctcttgaaacttacaaaagaaaactgctgggtgaaaagttggatgaatgttctattgaagaactgcatagcctggaggtcaagctggagagaagcctcattagcatcaggggaaggaagacaaagctgcttgaggagcaggttgccaaactgagagagaaggagatgaagctgcgcaaggacaatgaagagttacgcgaaaagtgtaagaatcagcctcccttgtctgctcctttgactgtccgggccgaagatgagaacccggaccgtaacatcaacaccaccaacgacaacatggatgtcgaaactgagctattcatagggctgcctggcagaagtcgctccagcggcggtgctgcagaagatagccaagcgatgccccattcttaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MVRGKTQMKRIENPTSRQVTFSKRRNGLLKKAFELSVLCDAEVA LIVFSPRGKLYEFASASTQKTIERYRTYTKENIGNKTVQQDIEQVKADADGLAKKLEA LETYKRKLLGEKLDECSIEELHSLEVKLERSLISIRGRKTKLLEEQVAKLREKEMKLR KDNEELREKCKNQPPLSAPLTVRAEDENPDRNINTTNDNMDVETELFIGLPGRSRSSG GAAEDSQAMPHS&lt;/aaseq&gt;|<br /> DNA = 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cgatcggcagactccactacccatctaaatctaaatcgagagatctcaagtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055336.1 RefSeq:Os03g0122600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Fangchao https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0122600&diff=171664 2014-05-24T07:10:04Z

<p>Fangchao: </p> <hr /> <div>OsMADS50 functions as a LD-specific flowering activator.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> [[File:Molecular network.jpg|right|thumb|150px|''Molecular network (from reference &lt;ref name=&quot;ref1&quot; /&gt;).'']]<br /> <br /> [[File:File:Schematic diagram of OsMADS50 genomic structure, positions of T-DNA insertional mutant alleles and flowering time of mutants.gif|right|thumb|150px|''Schematic diagram of OsMADS50 genomic structure &lt;ref name=&quot;ref3&quot; /&gt;).'']]<br /> <br /> OsMADS50 functions as a LD-specific flowering activator that functions only under LD conditions&lt;ref name=&quot;ref3&quot; /&gt;.<br /> <br /> OsMADS50 encodes MIKC-type MADS-box proteins . MADS-box genes play important roles in several developmental processes, e.g. control of flowering time, floral organ identity and fruit developmen.<br /> <br /> OsMADS50 serves as a flowering activator by controlling various floral regulators such as OsMADS1, OsMADS14, OsMADS15, OsMADS18 and Hd3a.It causing early flowering when overexpressed and delayed flowering when suppressed.OsMADS50 activates Ehd1 expression, and in turn Ehd1 activates RFT1 expression. RFT1 acts as the LD-specific florigen(from reference &lt;ref name=&quot;ref1&quot; /&gt;).<br /> <br /> <br /> ===Mutation===<br /> [[File:Phenotype comparison between OsMADS50 KO and WT plants.gif|left|thumb|200px|''Phenotype comparison between OsMADS50 KO and WT plants '']]<br /> <br /> OsMADS50 KO plants&lt;ref name=&quot;ref2&quot; /&gt;<br /> <br /> <br /> ===Expression===<br /> [[File:Expression.gif|right|thumb|150px|'' Expression profiles of OsMADS50 and other flowering regulators. (from reference &lt;ref name=&quot;ref2&quot; /&gt;).'']]<br /> <br /> RNA gel blot analysis revealed that OsMADS50 was variably expressed in most organs (Figure 6a). This gene was detected at a low level during the seedling stage, with transcripts increasing as the plant matured. In young panicles, expression was initially low, and continued to decline as these organs matured. RT-PCR analysis of leaves at four developmental stages confirmed the RNA gel blot analysis (Figure 6b). OsMADS50 transcript was detected at all four stages, with the expression level slightly increasing in 49-day-old plants compared with those that were 20 days old. The transcripts of Hd3a, OsMADS14, OsMADS15, and OsMADS18 increased gradually, reaching a maximum at 80 days&lt;ref name=&quot;ref2&quot; /&gt;.<br /> <br /> <br /> ===Evolution===<br /> [[File:Dendrigram.jpg|right|thumb|150px|'Dendrigram .'']]<br /> <br /> In rice, OsMADS50 is the protein most homologous with SOC1/AGL20, showing 50.6% identity, whereas OsMADS56 has 48.3% identity. Genome-wide phylogenic analysis showed that OsMADS50 is clustered with the SOC1-group MADS-box proteins.&lt;ref name=&quot;ref4&quot; /&gt;<br /> <br /> <br /> ===Knowledge Extension===<br /> [[File:PCE 2008 f8.gif|right|thumb|150px|'' Interaction analyses between OsMADS50 and OsMADS56&lt;ref name=&quot;ref2&quot; /&gt;.'']]<br /> <br /> OsMADS50 is the putative ortholog of SOC1/AGL20.<br /> <br /> OsMADS50 is upstream of OsMADS1, OsMADS14, OsMADS15, and OsMADS18.Expression analyses of the MADS-box genes in WT plants demonstrated that <br /> <br /> OsMADS50 acts upstream of the other MADS-box genes.<br /> <br /> OsMADS50 physically interacts with OsMADS56.Because both OsMADS50 and OsMADS56 appear to function as LD-specific regulators,the OsMADS50 KC region indeed binds to the OsMADS56 KC.&lt;ref name=&quot;ref3&quot; /&gt;<br /> <br /> OsMADS50 is epistatic to Hd3a.Three genes, OsGI, Hd1, and Hd3a, play major roles in the photoperiodic flowering pathway.The expression of OsGI and Hd1 was not affected by OsMADS50, thereby indicating that they act upstream of or in parallel with OsMADS50. In contrast, Hd3a was downregulated in the OsMADS50 KO plants, implying that OsMADS50 is epistatic to Hd3a&lt;ref name=&quot;ref2&quot; /&gt;.<br /> <br /> <br /> ==Labs working on this gene==<br /> Department of Life Science and Functional Genomic Center, Pohang University of Science and Technology (POSTECH)<br /> <br /> National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center,Nanjing Agricultural University<br /> <br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;STsuji H, Taoka K, Shimamoto K. Regulation of flowering in rice: two florigen genes, a complex gene network, and natural variation[J]. Current opinion in plant biology, 2011, 14(1): 45-52.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Lee S, Kim J, Han J J, et al. Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS‐LIKE 20 (SOC1/AGL20) ortholog in rice[J]. The Plant Journal, 2004, 38(5): 754-764.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref3&quot;&gt;RYU C H, Lee S, CHO L A E H, et al. OsMADS50 and OsMADS56 function antagonistically in regulating long day (LD)‐dependent flowering in rice[J]. Plant, cell &amp; environment, 2009, 32(10): 1412-1427.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref4&quot;&gt;Lee S Y, Jang S H, Jun S H, et al. Functional analysis of MADS-box genes expressed preferentially in vegetative tissues[C]//Advances in rice genetics, Los Baños, Laguna, Philippines, 22-27 October 2000. World Scientific Publishing Co. Pte. Ltd, 2003: 414-416.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0122600|<br /> Description = Transcription factor, MADS-box domain containing protein|<br /> Version = NM_001055336.1 GI:115450400 GeneID:4331445|<br /> Length = 29946 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0122600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:1248675..1278620|<br /> CDS = 1248915..1249100,1249297..1249338,1249458..1249499,1249599..1249698,1249789..1249850&lt;br&gt;,1250412..1250490,1278122..1278303|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:1248675..1278620<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:1248675..1278620<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggtgcgggggaagacgcagatgaagcggatagagaaccccacgagccgccaggtcaccttctccaagcgccgcaacggcctgctcaagaaggccttcgagctctccgtcctctgcgacgccgaggtcgcgctcatcgtcttctccccgcgcggcaagctctacgaattcgccagcgccagtacgcagaaaacaattgaacgctataggacgtatacaaaggaaaatatcggcaacaagacagtacagcaagatatagagcaagtaaaagctgacgctgatggtttggcaaagaaacttgaagctcttgaaacttacaaaagaaaactgctgggtgaaaagttggatgaatgttctattgaagaactgcatagcctggaggtcaagctggagagaagcctcattagcatcaggggaaggaagacaaagctgcttgaggagcaggttgccaaactgagagagaaggagatgaagctgcgcaaggacaatgaagagttacgcgaaaagtgtaagaatcagcctcccttgtctgctcctttgactgtccgggccgaagatgagaacccggaccgtaacatcaacaccaccaacgacaacatggatgtcgaaactgagctattcatagggctgcctggcagaagtcgctccagcggcggtgctgcagaagatagccaagcgatgccccattcttaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MVRGKTQMKRIENPTSRQVTFSKRRNGLLKKAFELSVLCDAEVA LIVFSPRGKLYEFASASTQKTIERYRTYTKENIGNKTVQQDIEQVKADADGLAKKLEA LETYKRKLLGEKLDECSIEELHSLEVKLERSLISIRGRKTKLLEEQVAKLREKEMKLR KDNEELREKCKNQPPLSAPLTVRAEDENPDRNINTTNDNMDVETELFIGLPGRSRSSG GAAEDSQAMPHS&lt;/aaseq&gt;|<br /> DNA = 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cgatcggcagactccactacccatctaaatctaaatcgagagatctcaagtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055336.1 RefSeq:Os03g0122600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Fangchao https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0122600&diff=171662 2014-05-24T07:07:48Z

<p>Fangchao: </p> <hr /> <div>OsMADS50 functions as a LD-specific flowering activator.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> [[File:Molecular network.jpg|right|thumb|150px|''Molecular network (from reference &lt;ref name=&quot;ref1&quot; /&gt;).'']]<br /> <br /> [[File:File:Schematic diagram of OsMADS50 genomic structure, positions of T-DNA insertional mutant alleles and flowering time of mutants.gif|150px|''Phenotype comparison between OsMADS50 KO and WT plants &lt;ref name=&quot;ref3&quot; /&gt;).'']]<br /> <br /> OsMADS50 functions as a LD-specific flowering activator that functions only under LD conditions&lt;ref name=&quot;ref3&quot; /&gt;.<br /> <br /> OsMADS50 encodes MIKC-type MADS-box proteins . MADS-box genes play important roles in several developmental processes, e.g. control of flowering time, floral organ identity and fruit developmen.<br /> <br /> OsMADS50 serves as a flowering activator by controlling various floral regulators such as OsMADS1, OsMADS14, OsMADS15, OsMADS18 and Hd3a.It causing early flowering when overexpressed and delayed flowering when suppressed.OsMADS50 activates Ehd1 expression, and in turn Ehd1 activates RFT1 expression. RFT1 acts as the LD-specific florigen(from reference &lt;ref name=&quot;ref1&quot; /&gt;).<br /> <br /> <br /> ===Mutation===<br /> [[File:Phenotype comparison between OsMADS50 KO and WT plants.gif|left|thumb|200px|''Phenotype comparison between OsMADS50 KO and WT plants '']]<br /> <br /> OsMADS50 KO plants&lt;ref name=&quot;ref2&quot; /&gt;<br /> <br /> <br /> ===Expression===<br /> [[File:Expression.gif|right|thumb|150px|'' Expression profiles of OsMADS50 and other flowering regulators. (from reference &lt;ref name=&quot;ref2&quot; /&gt;).'']]<br /> <br /> RNA gel blot analysis revealed that OsMADS50 was variably expressed in most organs (Figure 6a). This gene was detected at a low level during the seedling stage, with transcripts increasing as the plant matured. In young panicles, expression was initially low, and continued to decline as these organs matured. RT-PCR analysis of leaves at four developmental stages confirmed the RNA gel blot analysis (Figure 6b). OsMADS50 transcript was detected at all four stages, with the expression level slightly increasing in 49-day-old plants compared with those that were 20 days old. The transcripts of Hd3a, OsMADS14, OsMADS15, and OsMADS18 increased gradually, reaching a maximum at 80 days&lt;ref name=&quot;ref2&quot; /&gt;.<br /> <br /> <br /> ===Evolution===<br /> [[File:Dendrigram.jpg|right|thumb|150px|'Dendrigram .'']]<br /> <br /> In rice, OsMADS50 is the protein most homologous with SOC1/AGL20, showing 50.6% identity, whereas OsMADS56 has 48.3% identity. Genome-wide phylogenic analysis showed that OsMADS50 is clustered with the SOC1-group MADS-box proteins.&lt;ref name=&quot;ref4&quot; /&gt;<br /> <br /> <br /> ===Knowledge Extension===<br /> [[File:PCE 2008 f8.gif|right|thumb|150px|'' Interaction analyses between OsMADS50 and OsMADS56&lt;ref name=&quot;ref2&quot; /&gt;.'']]<br /> <br /> OsMADS50 is the putative ortholog of SOC1/AGL20.<br /> <br /> OsMADS50 is upstream of OsMADS1, OsMADS14, OsMADS15, and OsMADS18.Expression analyses of the MADS-box genes in WT plants demonstrated that <br /> <br /> OsMADS50 acts upstream of the other MADS-box genes.<br /> <br /> OsMADS50 physically interacts with OsMADS56.Because both OsMADS50 and OsMADS56 appear to function as LD-specific regulators,the OsMADS50 KC region indeed binds to the OsMADS56 KC.&lt;ref name=&quot;ref3&quot; /&gt;<br /> <br /> OsMADS50 is epistatic to Hd3a.Three genes, OsGI, Hd1, and Hd3a, play major roles in the photoperiodic flowering pathway.The expression of OsGI and Hd1 was not affected by OsMADS50, thereby indicating that they act upstream of or in parallel with OsMADS50. In contrast, Hd3a was downregulated in the OsMADS50 KO plants, implying that OsMADS50 is epistatic to Hd3a&lt;ref name=&quot;ref2&quot; /&gt;.<br /> <br /> <br /> ==Labs working on this gene==<br /> Department of Life Science and Functional Genomic Center, Pohang University of Science and Technology (POSTECH)<br /> <br /> National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center,Nanjing Agricultural University<br /> <br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;STsuji H, Taoka K, Shimamoto K. Regulation of flowering in rice: two florigen genes, a complex gene network, and natural variation[J]. Current opinion in plant biology, 2011, 14(1): 45-52.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Lee S, Kim J, Han J J, et al. Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS‐LIKE 20 (SOC1/AGL20) ortholog in rice[J]. The Plant Journal, 2004, 38(5): 754-764.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref3&quot;&gt;RYU C H, Lee S, CHO L A E H, et al. OsMADS50 and OsMADS56 function antagonistically in regulating long day (LD)‐dependent flowering in rice[J]. Plant, cell &amp; environment, 2009, 32(10): 1412-1427.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref4&quot;&gt;Lee S Y, Jang S H, Jun S H, et al. Functional analysis of MADS-box genes expressed preferentially in vegetative tissues[C]//Advances in rice genetics, Los Baños, Laguna, Philippines, 22-27 October 2000. World Scientific Publishing Co. Pte. Ltd, 2003: 414-416.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0122600|<br /> Description = Transcription factor, MADS-box domain containing protein|<br /> Version = NM_001055336.1 GI:115450400 GeneID:4331445|<br /> Length = 29946 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0122600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:1248675..1278620|<br /> CDS = 1248915..1249100,1249297..1249338,1249458..1249499,1249599..1249698,1249789..1249850&lt;br&gt;,1250412..1250490,1278122..1278303|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:1248675..1278620<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:1248675..1278620<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggtgcgggggaagacgcagatgaagcggatagagaaccccacgagccgccaggtcaccttctccaagcgccgcaacggcctgctcaagaaggccttcgagctctccgtcctctgcgacgccgaggtcgcgctcatcgtcttctccccgcgcggcaagctctacgaattcgccagcgccagtacgcagaaaacaattgaacgctataggacgtatacaaaggaaaatatcggcaacaagacagtacagcaagatatagagcaagtaaaagctgacgctgatggtttggcaaagaaacttgaagctcttgaaacttacaaaagaaaactgctgggtgaaaagttggatgaatgttctattgaagaactgcatagcctggaggtcaagctggagagaagcctcattagcatcaggggaaggaagacaaagctgcttgaggagcaggttgccaaactgagagagaaggagatgaagctgcgcaaggacaatgaagagttacgcgaaaagtgtaagaatcagcctcccttgtctgctcctttgactgtccgggccgaagatgagaacccggaccgtaacatcaacaccaccaacgacaacatggatgtcgaaactgagctattcatagggctgcctggcagaagtcgctccagcggcggtgctgcagaagatagccaagcgatgccccattcttaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MVRGKTQMKRIENPTSRQVTFSKRRNGLLKKAFELSVLCDAEVA LIVFSPRGKLYEFASASTQKTIERYRTYTKENIGNKTVQQDIEQVKADADGLAKKLEA LETYKRKLLGEKLDECSIEELHSLEVKLERSLISIRGRKTKLLEEQVAKLREKEMKLR KDNEELREKCKNQPPLSAPLTVRAEDENPDRNINTTNDNMDVETELFIGLPGRSRSSG GAAEDSQAMPHS&lt;/aaseq&gt;|<br /> DNA = 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cgatcggcagactccactacccatctaaatctaaatcgagagatctcaagtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055336.1 RefSeq:Os03g0122600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Fangchao https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0122600&diff=171652 2014-05-24T06:59:27Z

<p>Fangchao: </p> <hr /> <div>OsMADS50 functions as a LD-specific flowering activator.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> [[File:Molecular network.jpg|right|thumb|150px|''Molecular network (from reference &lt;ref name=&quot;ref1&quot; /&gt;).'']]<br /> <br /> [[File:File:Schematic diagram of OsMADS50 genomic structure, positions of T-DNA insertional mutant alleles and flowering time of mutants.gif|150px|''Phenotype comparison between OsMADS50 KO and WT plants &lt;ref name=&quot;ref3&quot; /&gt;).'']]<br /> <br /> OsMADS50 functions as a LD-specific flowering activator that functions only under LD conditions&lt;ref name=&quot;ref3&quot; /&gt;.OsMADS50 encodes MIKC-type MADS-box proteins . MADS-box genes play important roles in several developmental processes, e.g. control of flowering time, floral organ identity and fruit developmen.OsMADS50 serves as a flowering activator by controlling various floral regulators such as OsMADS1, OsMADS14, OsMADS15, OsMADS18 and Hd3a.It causing early flowering when overexpressed and delayed flowering when suppressed.OsMADS50 activates Ehd1 expression, and in turn Ehd1 activates RFT1 expression. RFT1 acts as the LD-specific florigen(from reference &lt;ref name=&quot;ref1&quot; /&gt;).<br /> <br /> <br /> ===Mutation===<br /> [[File:Phenotype comparison between OsMADS50 KO and WT plants.gif|right|thumb|150px|''Phenotype comparison between OsMADS50 KO and WT plants (from reference &lt;ref name=&quot;ref2&quot; /&gt;).'']]<br /> <br /> OsMADS50 KO plants<br /> <br /> <br /> ===Expression===<br /> [[File:Expression.gif|right|thumb|150px|'' Expression profiles of OsMADS50 and other flowering regulators. (from reference &lt;ref name=&quot;ref2&quot; /&gt;).'']]<br /> <br /> RNA gel blot analysis revealed that OsMADS50 was variably expressed in most organs (Figure 6a). This gene was detected at a low level during the seedling stage, with transcripts increasing as the plant matured. In young panicles, expression was initially low, and continued to decline as these organs matured. RT-PCR analysis of leaves at four developmental stages confirmed the RNA gel blot analysis (Figure 6b). OsMADS50 transcript was detected at all four stages, with the expression level slightly increasing in 49-day-old plants compared with those that were 20 days old. The transcripts of Hd3a, OsMADS14, OsMADS15, and OsMADS18 increased gradually, reaching a maximum at 80 days&lt;ref name=&quot;ref2&quot; /&gt;.<br /> <br /> <br /> ===Evolution===<br /> [[File:Dendrigram.jpg|right|thumb|150px|'Dendrigram .'']]<br /> <br /> In rice, OsMADS50 is the protein most homologous with SOC1/AGL20, showing 50.6% identity, whereas OsMADS56 has 48.3% identity. Genome-wide phylogenic analysis showed that OsMADS50 is clustered with the SOC1-group MADS-box proteins.&lt;ref name=&quot;ref4&quot; /&gt;<br /> <br /> <br /> ===Knowledge Extension===<br /> [[File:PCE 2008 f8.gif|right|thumb|150px|'' Interaction analyses between OsMADS50 and OsMADS56&lt;ref name=&quot;ref2&quot; /&gt;.'']]<br /> <br /> OsMADS50 is the putative ortholog of SOC1/AGL20.<br /> <br /> OsMADS50 is upstream of OsMADS1, OsMADS14, OsMADS15, and OsMADS18.Expression analyses of the MADS-box genes in WT plants demonstrated that <br /> <br /> OsMADS50 acts upstream of the other MADS-box genes.<br /> <br /> OsMADS50 physically interacts with OsMADS56.Because both OsMADS50 and OsMADS56 appear to function as LD-specific regulators,the OsMADS50 KC region indeed binds to the OsMADS56 KC.&lt;ref name=&quot;ref3&quot; /&gt;<br /> <br /> OsMADS50 is epistatic to Hd3a.Three genes, OsGI, Hd1, and Hd3a, play major roles in the photoperiodic flowering pathway.The expression of OsGI and Hd1 was not affected by OsMADS50, thereby indicating that they act upstream of or in parallel with OsMADS50. In contrast, Hd3a was downregulated in the OsMADS50 KO plants, implying that OsMADS50 is epistatic to Hd3a&lt;ref name=&quot;ref2&quot; /&gt;.<br /> <br /> <br /> ==Labs working on this gene==<br /> Department of Life Science and Functional Genomic Center, Pohang University of Science and Technology (POSTECH)<br /> National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center,Nanjing Agricultural University<br /> <br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;STsuji H, Taoka K, Shimamoto K. Regulation of flowering in rice: two florigen genes, a complex gene network, and natural variation[J]. Current opinion in plant biology, 2011, 14(1): 45-52.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Lee S, Kim J, Han J J, et al. Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS‐LIKE 20 (SOC1/AGL20) ortholog in rice[J]. The Plant Journal, 2004, 38(5): 754-764.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref3&quot;&gt;RYU C H, Lee S, CHO L A E H, et al. OsMADS50 and OsMADS56 function antagonistically in regulating long day (LD)‐dependent flowering in rice[J]. Plant, cell &amp; environment, 2009, 32(10): 1412-1427.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref4&quot;&gt;Lee S Y, Jang S H, Jun S H, et al. Functional analysis of MADS-box genes expressed preferentially in vegetative tissues[C]//Advances in rice genetics, Los Baños, Laguna, Philippines, 22-27 October 2000. World Scientific Publishing Co. Pte. Ltd, 2003: 414-416.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0122600|<br /> Description = Transcription factor, MADS-box domain containing protein|<br /> Version = NM_001055336.1 GI:115450400 GeneID:4331445|<br /> Length = 29946 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0122600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:1248675..1278620|<br /> CDS = 1248915..1249100,1249297..1249338,1249458..1249499,1249599..1249698,1249789..1249850&lt;br&gt;,1250412..1250490,1278122..1278303|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:1248675..1278620<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:1248675..1278620<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggtgcgggggaagacgcagatgaagcggatagagaaccccacgagccgccaggtcaccttctccaagcgccgcaacggcctgctcaagaaggccttcgagctctccgtcctctgcgacgccgaggtcgcgctcatcgtcttctccccgcgcggcaagctctacgaattcgccagcgccagtacgcagaaaacaattgaacgctataggacgtatacaaaggaaaatatcggcaacaagacagtacagcaagatatagagcaagtaaaagctgacgctgatggtttggcaaagaaacttgaagctcttgaaacttacaaaagaaaactgctgggtgaaaagttggatgaatgttctattgaagaactgcatagcctggaggtcaagctggagagaagcctcattagcatcaggggaaggaagacaaagctgcttgaggagcaggttgccaaactgagagagaaggagatgaagctgcgcaaggacaatgaagagttacgcgaaaagtgtaagaatcagcctcccttgtctgctcctttgactgtccgggccgaagatgagaacccggaccgtaacatcaacaccaccaacgacaacatggatgtcgaaactgagctattcatagggctgcctggcagaagtcgctccagcggcggtgctgcagaagatagccaagcgatgccccattcttaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MVRGKTQMKRIENPTSRQVTFSKRRNGLLKKAFELSVLCDAEVA LIVFSPRGKLYEFASASTQKTIERYRTYTKENIGNKTVQQDIEQVKADADGLAKKLEA LETYKRKLLGEKLDECSIEELHSLEVKLERSLISIRGRKTKLLEEQVAKLREKEMKLR KDNEELREKCKNQPPLSAPLTVRAEDENPDRNINTTNDNMDVETELFIGLPGRSRSSG GAAEDSQAMPHS&lt;/aaseq&gt;|<br /> DNA = 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cgatcggcagactccactacccatctaaatctaaatcgagagatctcaagtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055336.1 RefSeq:Os03g0122600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Fangchao https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0122600&diff=171645 2014-05-24T06:53:35Z

<p>Fangchao: </p> <hr /> <div>OsMADS50 functions as a LD-specific flowering activator.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> [[File:Molecular network.jpg|right|thumb|150px|''Molecular network (from reference &lt;ref name=&quot;ref1&quot; /&gt;).'']]<br /> <br /> [[File:File:Schematic diagram of OsMADS50 genomic structure, positions of T-DNA insertional mutant alleles and flowering time of mutants.gif|150px|''Phenotype comparison between OsMADS50 KO and WT plants &lt;ref name=&quot;ref3&quot; /&gt;).'']]<br /> <br /> OsMADS50 functions as a LD-specific flowering activator that functions only under LD conditions&lt;ref name=&quot;ref3&quot; /&gt;.OsMADS50 encodes MIKC-type MADS-box proteins . MADS-box genes play important roles in several developmental processes, e.g. control of flowering time, floral organ identity and fruit developmen.OsMADS50 serves as a flowering activator by controlling various floral regulators such as OsMADS1, OsMADS14, OsMADS15, OsMADS18 and Hd3a.It causing early flowering when overexpressed and delayed flowering when suppressed.OsMADS50 activates Ehd1 expression, and in turn Ehd1 activates RFT1 expression. RFT1 acts as the LD-specific florigen(from reference &lt;ref name=&quot;ref1&quot; /&gt;).<br /> <br /> ===Mutation===<br /> [[File:Phenotype comparison between OsMADS50 KO and WT plants.gif|right|thumb|150px|''Phenotype comparison between OsMADS50 KO and WT plants (from reference &lt;ref name=&quot;ref2&quot; /&gt;).'']]<br /> OsMADS50 KO plants<br /> <br /> ===Expression===<br /> [[File:Expression.gif|right|thumb|150px|'' Expression profiles of OsMADS50 and other flowering regulators. (from reference &lt;ref name=&quot;ref2&quot; /&gt;).'']]<br /> <br /> RNA gel blot analysis revealed that OsMADS50 was variably expressed in most organs (Figure 6a). This gene was detected at a low level during the seedling stage, with transcripts increasing as the plant matured. In young panicles, expression was initially low, and continued to decline as these organs matured. RT-PCR analysis of leaves at four developmental stages confirmed the RNA gel blot analysis (Figure 6b). OsMADS50 transcript was detected at all four stages, with the expression level slightly increasing in 49-day-old plants compared with those that were 20 days old. The transcripts of Hd3a, OsMADS14, OsMADS15, and OsMADS18 increased gradually, reaching a maximum at 80 days&lt;ref name=&quot;ref2&quot; /&gt;.<br /> <br /> ===Evolution===<br /> [[File:Dendrigram.jpg|right|thumb|150px|'Dendrigram .'']]<br /> <br /> In rice, OsMADS50 is the protein most homologous with SOC1/AGL20, showing 50.6% identity, whereas OsMADS56 has 48.3% identity. Genome-wide phylogenic analysis showed that OsMADS50 is clustered with the SOC1-group MADS-box proteins.&lt;ref name=&quot;ref4&quot; /&gt;<br /> <br /> ===Knowledge Extension===<br /> [[File:PCE 2008 f8.gif|right|thumb|150px|'' Interaction analyses between OsMADS50 and OsMADS56&lt;ref name=&quot;ref2&quot; /&gt;.'']]<br /> <br /> OsMADS50 is the putative ortholog of SOC1/AGL20.<br /> OsMADS50 is upstream of OsMADS1, OsMADS14, OsMADS15, and OsMADS18.Expression analyses of the MADS-box genes in WT plants demonstrated that OsMADS50 acts upstream of the other MADS-box genes.<br /> OsMADS50 physically interacts with OsMADS56.Because both OsMADS50 and OsMADS56 appear to function as LD-specific regulators,the OsMADS50 KC region indeed binds to the OsMADS56 KC.&lt;ref name=&quot;ref3&quot; /&gt;<br /> <br /> OsMADS50 is epistatic to Hd3a.Three genes, OsGI, Hd1, and Hd3a, play major roles in the photoperiodic flowering pathway.The expression of OsGI and Hd1 was not affected by OsMADS50, thereby indicating that they act upstream of or in parallel with OsMADS50. In contrast, Hd3a was downregulated in the OsMADS50 KO plants, implying that OsMADS50 is epistatic to Hd3a&lt;ref name=&quot;ref2&quot; /&gt;.<br /> <br /> ==Labs working on this gene==<br /> Department of Life Science and Functional Genomic Center, Pohang University of Science and Technology (POSTECH)<br /> National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center,Nanjing Agricultural University<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;STsuji H, Taoka K, Shimamoto K. Regulation of flowering in rice: two florigen genes, a complex gene network, and natural variation[J]. Current opinion in plant biology, 2011, 14(1): 45-52.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Lee S, Kim J, Han J J, et al. Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS‐LIKE 20 (SOC1/AGL20) ortholog in rice[J]. The Plant Journal, 2004, 38(5): 754-764.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref3&quot;&gt;RYU C H, Lee S, CHO L A E H, et al. OsMADS50 and OsMADS56 function antagonistically in regulating long day (LD)‐dependent flowering in rice[J]. Plant, cell &amp; environment, 2009, 32(10): 1412-1427.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref4&quot;&gt;Lee S Y, Jang S H, Jun S H, et al. Functional analysis of MADS-box genes expressed preferentially in vegetative tissues[C]//Advances in rice genetics, Los Baños, Laguna, Philippines, 22-27 October 2000. World Scientific Publishing Co. Pte. Ltd, 2003: 414-416.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0122600|<br /> Description = Transcription factor, MADS-box domain containing protein|<br /> Version = NM_001055336.1 GI:115450400 GeneID:4331445|<br /> Length = 29946 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0122600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:1248675..1278620|<br /> CDS = 1248915..1249100,1249297..1249338,1249458..1249499,1249599..1249698,1249789..1249850&lt;br&gt;,1250412..1250490,1278122..1278303|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:1248675..1278620<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:1248675..1278620<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggtgcgggggaagacgcagatgaagcggatagagaaccccacgagccgccaggtcaccttctccaagcgccgcaacggcctgctcaagaaggccttcgagctctccgtcctctgcgacgccgaggtcgcgctcatcgtcttctccccgcgcggcaagctctacgaattcgccagcgccagtacgcagaaaacaattgaacgctataggacgtatacaaaggaaaatatcggcaacaagacagtacagcaagatatagagcaagtaaaagctgacgctgatggtttggcaaagaaacttgaagctcttgaaacttacaaaagaaaactgctgggtgaaaagttggatgaatgttctattgaagaactgcatagcctggaggtcaagctggagagaagcctcattagcatcaggggaaggaagacaaagctgcttgaggagcaggttgccaaactgagagagaaggagatgaagctgcgcaaggacaatgaagagttacgcgaaaagtgtaagaatcagcctcccttgtctgctcctttgactgtccgggccgaagatgagaacccggaccgtaacatcaacaccaccaacgacaacatggatgtcgaaactgagctattcatagggctgcctggcagaagtcgctccagcggcggtgctgcagaagatagccaagcgatgccccattcttaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MVRGKTQMKRIENPTSRQVTFSKRRNGLLKKAFELSVLCDAEVA LIVFSPRGKLYEFASASTQKTIERYRTYTKENIGNKTVQQDIEQVKADADGLAKKLEA LETYKRKLLGEKLDECSIEELHSLEVKLERSLISIRGRKTKLLEEQVAKLREKEMKLR KDNEELREKCKNQPPLSAPLTVRAEDENPDRNINTTNDNMDVETELFIGLPGRSRSSG GAAEDSQAMPHS&lt;/aaseq&gt;|<br /> DNA = 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cgatcggcagactccactacccatctaaatctaaatcgagagatctcaagtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055336.1 RefSeq:Os03g0122600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Fangchao https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0122600&diff=171636 2014-05-24T06:41:33Z

<p>Fangchao: </p> <hr /> <div>OsMADS50 functions as a LD-specific flowering activator.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> [[File:Molecular network.jpg|right|thumb|150px|''Molecular network (from reference &lt;ref name=&quot;ref1&quot; /&gt;).'']]<br /> <br /> [[File:File:Schematic diagram of OsMADS50 genomic structure, positions of T-DNA insertional mutant alleles and flowering time of mutants.gif|150px|''Phenotype comparison between OsMADS50 KO and WT plants &lt;ref name=&quot;ref3&quot; /&gt;).'']]<br /> <br /> OsMADS50 functions as a LD-specific flowering activator that functions only under LD conditions&lt;ref name=&quot;ref3&quot; /&gt;.OsMADS50 encodes MIKC-type MADS-box proteins . MADS-box genes play important roles in several developmental processes, e.g. control of flowering time, floral organ identity and fruit developmen.OsMADS50 serves as a flowering activator by controlling various floral regulators such as OsMADS1, OsMADS14, OsMADS15, OsMADS18 and Hd3a.It causing early flowering when overexpressed and delayed flowering when suppressed.OsMADS50 activates Ehd1 expression, and in turn Ehd1 activates RFT1 expression. RFT1 acts as the LD-specific florigen(from reference &lt;ref name=&quot;ref1&quot; /&gt;).<br /> <br /> ===Mutation===<br /> [[File:Phenotype comparison between OsMADS50 KO and WT plants.gif|right|thumb|150px|''Phenotype comparison between OsMADS50 KO and WT plants &lt;ref name=&quot;ref2&quot; /&gt;).'']]<br /> OsMADS50 KO plants<br /> <br /> ===Expression===<br /> [[File:Expression.gif|right|thumb|150px|'' Expression profiles of OsMADS50 and other flowering regulators. (from reference &lt;ref name=&quot;ref2&quot; /&gt;).'']]<br /> <br /> RNA gel blot analysis revealed that OsMADS50 was variably expressed in most organs (Figure 6a). This gene was detected at a low level during the seedling stage, with transcripts increasing as the plant matured. In young panicles, expression was initially low, and continued to decline as these organs matured. RT-PCR analysis of leaves at four developmental stages confirmed the RNA gel blot analysis (Figure 6b). OsMADS50 transcript was detected at all four stages, with the expression level slightly increasing in 49-day-old plants compared with those that were 20 days old. The transcripts of Hd3a, OsMADS14, OsMADS15, and OsMADS18 increased gradually, reaching a maximum at 80 days.(from reference &lt;ref name=&quot;ref2&quot; /&gt;)<br /> <br /> ===Evolution===<br /> [[File:Dendrigram.jpg|right|thumb|150px|'Dendrigram .'']]<br /> <br /> In rice, OsMADS50 is the protein most homologous with SOC1/AGL20, showing 50.6% identity, whereas OsMADS56 has 48.3% identity. Genome-wide phylogenic analysis showed that OsMADS50 is clustered with the SOC1-group MADS-box proteins.(from reference &lt;ref name=&quot;ref4Y&quot; /&gt;)<br /> <br /> ===Knowledge Extension===<br /> [[File:PCE 2008 f8.gif|right|thumb|150px|'' Interaction analyses between OsMADS50 and OsMADS56 (from reference &lt;ref name=&quot;ref2&quot; /&gt;).'']]<br /> <br /> OsMADS50 is the putative ortholog of SOC1/AGL20.<br /> OsMADS50 is upstream of OsMADS1, OsMADS14, OsMADS15, and OsMADS18.Expression analyses of the MADS-box genes in WT plants demonstrated that OsMADS50 acts upstream of the other MADS-box genes.<br /> OsMADS50 physically interacts with OsMADS56.Because both OsMADS50 and OsMADS56 appear to function as LD-specific regulators,the OsMADS50 KC region indeed binds to the OsMADS56 KC.(from reference &lt;ref name=&quot;ref3&quot; /&gt;)<br /> <br /> OsMADS50 is epistatic to Hd3a.Three genes, OsGI, Hd1, and Hd3a, play major roles in the photoperiodic flowering pathway.The expression of OsGI and Hd1 was not affected by OsMADS50, thereby indicating that they act upstream of or in parallel with OsMADS50. In contrast, Hd3a was downregulated in the OsMADS50 KO plants, implying that OsMADS50 is epistatic to Hd3a. (from reference &lt;ref name=&quot;ref2&quot; /&gt;)<br /> <br /> ==Labs working on this gene==<br /> Department of Life Science and Functional Genomic Center, Pohang University of Science and Technology (POSTECH)<br /> National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center,Nanjing Agricultural University<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;STsuji H, Taoka K, Shimamoto K. Regulation of flowering in rice: two florigen genes, a complex gene network, and natural variation[J]. Current opinion in plant biology, 2011, 14(1): 45-52..&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;Lee S, Kim J, Han J J, et al. Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS‐LIKE 20 (SOC1/AGL20) ortholog in rice[J]. The Plant Journal, 2004, 38(5): 754-764.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref3&quot;&gt;RYU C H, Lee S, CHO L A E H, et al. OsMADS50 and OsMADS56 function antagonistically in regulating long day (LD)‐dependent flowering in rice[J]. Plant, cell &amp; environment, 2009, 32(10): 1412-1427.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref4&quot;&gt;Lee S Y, Jang S H, Jun S H, et al. Functional analysis of MADS-box genes expressed preferentially in vegetative tissues[C]//Advances in rice genetics, Los Baños, Laguna, Philippines, 22-27 October 2000. World Scientific Publishing Co. Pte. Ltd, 2003: 414-416.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0122600|<br /> Description = Transcription factor, MADS-box domain containing protein|<br /> Version = NM_001055336.1 GI:115450400 GeneID:4331445|<br /> Length = 29946 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0122600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:1248675..1278620|<br /> CDS = 1248915..1249100,1249297..1249338,1249458..1249499,1249599..1249698,1249789..1249850&lt;br&gt;,1250412..1250490,1278122..1278303|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:1248675..1278620<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:1248675..1278620<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggtgcgggggaagacgcagatgaagcggatagagaaccccacgagccgccaggtcaccttctccaagcgccgcaacggcctgctcaagaaggccttcgagctctccgtcctctgcgacgccgaggtcgcgctcatcgtcttctccccgcgcggcaagctctacgaattcgccagcgccagtacgcagaaaacaattgaacgctataggacgtatacaaaggaaaatatcggcaacaagacagtacagcaagatatagagcaagtaaaagctgacgctgatggtttggcaaagaaacttgaagctcttgaaacttacaaaagaaaactgctgggtgaaaagttggatgaatgttctattgaagaactgcatagcctggaggtcaagctggagagaagcctcattagcatcaggggaaggaagacaaagctgcttgaggagcaggttgccaaactgagagagaaggagatgaagctgcgcaaggacaatgaagagttacgcgaaaagtgtaagaatcagcctcccttgtctgctcctttgactgtccgggccgaagatgagaacccggaccgtaacatcaacaccaccaacgacaacatggatgtcgaaactgagctattcatagggctgcctggcagaagtcgctccagcggcggtgctgcagaagatagccaagcgatgccccattcttaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MVRGKTQMKRIENPTSRQVTFSKRRNGLLKKAFELSVLCDAEVA LIVFSPRGKLYEFASASTQKTIERYRTYTKENIGNKTVQQDIEQVKADADGLAKKLEA LETYKRKLLGEKLDECSIEELHSLEVKLERSLISIRGRKTKLLEEQVAKLREKEMKLR KDNEELREKCKNQPPLSAPLTVRAEDENPDRNINTTNDNMDVETELFIGLPGRSRSSG GAAEDSQAMPHS&lt;/aaseq&gt;|<br /> DNA = 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cgatcggcagactccactacccatctaaatctaaatcgagagatctcaagtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055336.1 RefSeq:Os03g0122600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Fangchao https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0122600&diff=171635 2014-05-24T06:36:55Z

<p>Fangchao: </p> <hr /> <div>OsMADS50 functions as a LD-specific flowering activator.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> [[File:Molecular network.jpg|right|thumb|150px|''Molecular network (from reference &lt;ref name=&quot;ref1&quot; /&gt;).'']]<br /> <br /> [[File:File:Schematic diagram of OsMADS50 genomic structure, positions of T-DNA insertional mutant alleles and flowering time of mutants.gif|150px|''Phenotype comparison between OsMADS50 KO and WT plants &lt;ref name=&quot;ref3&quot; /&gt;).'']]<br /> <br /> OsMADS50 functions as a LD-specific flowering activator that functions only under LD conditions&lt;ref name=&quot;ref3&quot; /&gt;.OsMADS50 encodes MIKC-type MADS-box proteins . MADS-box genes play important roles in several developmental processes, e.g. control of flowering time, floral organ identity and fruit developmen.OsMADS50 serves as a flowering activator by controlling various floral regulators such as OsMADS1, OsMADS14, OsMADS15, OsMADS18 and Hd3a.It causing early flowering when overexpressed and delayed flowering when suppressed.OsMADS50 activates Ehd1 expression, and in turn Ehd1 activates RFT1 expression. RFT1 acts as the LD-specific florigen(from reference &lt;ref name=&quot;ref1&quot; /&gt;).<br /> <br /> ===Mutation===<br /> [[File:Phenotype comparison between OsMADS50 KO and WT plants.|right|thumb|gif|150px|''Phenotype comparison between OsMADS50 KO and WT plants &lt;ref name=&quot;ref2&quot; /&gt;).'']]<br /> OsMADS50 KO plants<br /> <br /> ===Expression===<br /> [[File:Expression.gif|right|thumb|150px|'' Expression profiles of OsMADS50 and other flowering regulators. (from reference &lt;ref name=&quot;ref2&quot; /&gt;).'']]<br /> <br /> RNA gel blot analysis revealed that OsMADS50 was variably expressed in most organs (Figure 6a). This gene was detected at a low level during the seedling stage, with transcripts increasing as the plant matured. In young panicles, expression was initially low, and continued to decline as these organs matured. RT-PCR analysis of leaves at four developmental stages confirmed the RNA gel blot analysis (Figure 6b). OsMADS50 transcript was detected at all four stages, with the expression level slightly increasing in 49-day-old plants compared with those that were 20 days old. The transcripts of Hd3a, OsMADS14, OsMADS15, and OsMADS18 increased gradually, reaching a maximum at 80 days.(from reference &lt;ref name=&quot;ref2&quot; /&gt;)<br /> <br /> ===Evolution===<br /> [[File:Dendrigram.jpg|right|thumb|150px|'Dendrigram .'']]<br /> <br /> In rice, OsMADS50 is the protein most homologous with SOC1/AGL20, showing 50.6% identity, whereas OsMADS56 has 48.3% identity. Genome-wide phylogenic analysis showed that OsMADS50 is clustered with the SOC1-group MADS-box proteins.(from reference &lt;ref name=&quot;ref4Y&quot; /&gt;)<br /> <br /> ===Knowledge Extension===<br /> [[File:PCE 2008 f8.gif|right|thumb|150px|'' Interaction analyses between OsMADS50 and OsMADS56 (from reference &lt;ref name=&quot;ref2&quot; /&gt;).'']]<br /> <br /> OsMADS50 is the putative ortholog of SOC1/AGL20.<br /> OsMADS50 is upstream of OsMADS1, OsMADS14, OsMADS15, and OsMADS18.Expression analyses of the MADS-box genes in WT plants demonstrated that OsMADS50 acts upstream of the other MADS-box genes.<br /> OsMADS50 physically interacts with OsMADS56.Because both OsMADS50 and OsMADS56 appear to function as LD-specific regulators,the OsMADS50 KC region indeed binds to the OsMADS56 KC.(from reference &lt;ref name=&quot;ref3&quot; /&gt;)<br /> <br /> OsMADS50 is epistatic to Hd3a.Three genes, OsGI, Hd1, and Hd3a, play major roles in the photoperiodic flowering pathway.The expression of OsGI and Hd1 was not affected by OsMADS50, thereby indicating that they act upstream of or in parallel with OsMADS50. In contrast, Hd3a was downregulated in the OsMADS50 KO plants, implying that OsMADS50 is epistatic to Hd3a. (from reference &lt;ref name=&quot;ref2&quot; /&gt;)<br /> <br /> ==Labs working on this gene==<br /> Department of Life Science and Functional Genomic Center, Pohang University of Science and Technology (POSTECH)<br /> National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center,Nanjing Agricultural University<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;STsuji H, Taoka K, Shimamoto K. Regulation of flowering in rice: two florigen genes, a complex gene network, and natural variation[J]. Current opinion in plant biology, 2011, 14(1): 45-52..&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;Lee S, Kim J, Han J J, et al. Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS‐LIKE 20 (SOC1/AGL20) ortholog in rice[J]. The Plant Journal, 2004, 38(5): 754-764.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref3&quot;&gt;RYU C H, Lee S, CHO L A E H, et al. OsMADS50 and OsMADS56 function antagonistically in regulating long day (LD)‐dependent flowering in rice[J]. Plant, cell &amp; environment, 2009, 32(10): 1412-1427.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref4&quot;&gt;Lee S Y, Jang S H, Jun S H, et al. Functional analysis of MADS-box genes expressed preferentially in vegetative tissues[C]//Advances in rice genetics, Los Baños, Laguna, Philippines, 22-27 October 2000. World Scientific Publishing Co. Pte. Ltd, 2003: 414-416.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0122600|<br /> Description = Transcription factor, MADS-box domain containing protein|<br /> Version = NM_001055336.1 GI:115450400 GeneID:4331445|<br /> Length = 29946 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0122600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:1248675..1278620|<br /> CDS = 1248915..1249100,1249297..1249338,1249458..1249499,1249599..1249698,1249789..1249850&lt;br&gt;,1250412..1250490,1278122..1278303|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:1248675..1278620<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:1248675..1278620<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggtgcgggggaagacgcagatgaagcggatagagaaccccacgagccgccaggtcaccttctccaagcgccgcaacggcctgctcaagaaggccttcgagctctccgtcctctgcgacgccgaggtcgcgctcatcgtcttctccccgcgcggcaagctctacgaattcgccagcgccagtacgcagaaaacaattgaacgctataggacgtatacaaaggaaaatatcggcaacaagacagtacagcaagatatagagcaagtaaaagctgacgctgatggtttggcaaagaaacttgaagctcttgaaacttacaaaagaaaactgctgggtgaaaagttggatgaatgttctattgaagaactgcatagcctggaggtcaagctggagagaagcctcattagcatcaggggaaggaagacaaagctgcttgaggagcaggttgccaaactgagagagaaggagatgaagctgcgcaaggacaatgaagagttacgcgaaaagtgtaagaatcagcctcccttgtctgctcctttgactgtccgggccgaagatgagaacccggaccgtaacatcaacaccaccaacgacaacatggatgtcgaaactgagctattcatagggctgcctggcagaagtcgctccagcggcggtgctgcagaagatagccaagcgatgccccattcttaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MVRGKTQMKRIENPTSRQVTFSKRRNGLLKKAFELSVLCDAEVA LIVFSPRGKLYEFASASTQKTIERYRTYTKENIGNKTVQQDIEQVKADADGLAKKLEA LETYKRKLLGEKLDECSIEELHSLEVKLERSLISIRGRKTKLLEEQVAKLREKEMKLR KDNEELREKCKNQPPLSAPLTVRAEDENPDRNINTTNDNMDVETELFIGLPGRSRSSG GAAEDSQAMPHS&lt;/aaseq&gt;|<br /> DNA = 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cgatcggcagactccactacccatctaaatctaaatcgagagatctcaagtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055336.1 RefSeq:Os03g0122600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Fangchao https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:PCE_2008_f8.gif&diff=171616 2014-05-24T06:13:54Z

<p>Fangchao: Interaction analyses between OsMADS50 and OsMADS56. (a) Yeast two-hybrid assay. Two plasmids containing either AD or BD construct were introduced into yeast strain and transformants were grown on selective medium lacking Leu, Trp and His. OsMADS14-K bait </p> <hr /> <div> Interaction analyses between OsMADS50 and OsMADS56. (a) Yeast two-hybrid assay. Two plasmids containing either AD or BD construct were introduced into yeast strain and transformants were grown on selective medium lacking Leu, Trp and His. OsMADS14-K bait and OsMADS1-KC prey were used as positive control (Lim et al. 2000). (b, c) Co-immunoprecipitation assay between OsMADS50 and OsMADS56 (b), and for OsMADS50 itself and OsMADS56 itself (c). Crude extracts were prepared from 7 DAG seedling protoplasts that had been transfected with constructs expressing HA-tagged protein or Myc-tagged protein, alone or with both. Transiently expressed proteins were immunoprecipitated with anti-Myc antibody, separated by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) then detected with peroxidase-conjugated high-affinity anti-HA antibody. Expected sizes of HA-tagged OsMADS50 and OsMADS56 were 34.05 and 34.18 kDa, respectively. Inclusion or exclusion of plasmid is shown by (+) or (−). DAG, days after germination.</div>

Fangchao https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Schematic_diagram_of_OsMADS50_genomic_structure,_positions_of_T-DNA_insertional_mutant_alleles_and_flowering_time_of_mutants.gif&diff=171604 2014-05-24T05:44:30Z

<p>Fangchao: Schematic diagram of OsMADS50 genomic structure, positions of T-DNA insertional mutant alleles and flowering time of mutants. (a) OsMADS50 (Os03g0122600) consists of eight exons (boxes) and seven introns (lines between boxes). Gray boxes indicate 5′ and</p> <hr /> <div>Schematic diagram of OsMADS50 genomic structure, positions of T-DNA insertional mutant alleles and flowering time of mutants. (a) OsMADS50 (Os03g0122600) consists of eight exons (boxes) and seven introns (lines between boxes). Gray boxes indicate 5′ and 3′ untranslated regions. Triangles are T-DNAs, which were inserted into the fifth and third introns in osmads50-1 (Line 0-153-43) and osmads50-2 (Line 3A-03367), respectively. Arrows indicate F and R primers used for amplification of 344-base pair (bp) cDNA. LB is left border of T-DNA. (b) Days to heading in osmads50-1 and osmads50-2 plants under controlled LD (14 h light). (c) Days to heading in osmads50-1 and osmads50-2 plants under controlled SD (10 h light). Days to heading was scored when first panicle bolted. Error bars indicate standard deviations; n = 8 to 16 plants. DAG, days after germination; LD, long day; SD, short day; WT, wild type.</div>

Fangchao https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Phenotype_comparison_between_OsMADS50_KO_and_WT_plants.gif&diff=171599 2014-05-24T05:33:45Z

<p>Fangchao: uploaded a new version of &amp;quot;File:Phenotype comparison between OsMADS50 KO and WT plants.gif&amp;quot;: Photograph was taken when WT plant (W/W) flowered. The OsMADS50 KO (T/T) plant had not bolted.</p> <hr /> <div>Photograph was taken when WT plant (W/W) flowered. The OsMADS50 KO (T/T) plant had not bolted.</div>

Fangchao https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Dendrigram.jpg&diff=171596 2014-05-24T05:26:14Z

<p>Fangchao: Dendrigram of various MADS-box proteins of rice and Arabidopsis</p> <hr /> <div>Dendrigram of various MADS-box proteins of rice and Arabidopsis</div>

Fangchao https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Expression.gif&diff=171553 2014-05-24T03:42:59Z

<p>Fangchao: uploaded a new version of &amp;quot;File:Expression.gif&amp;quot;</p> <hr /> <div>OsDREB1A was rapidly induced by 15 min of exposure to MeJA and was gradually responsive to drought conditions</div>

Fangchao https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Phenotype_comparison_between_OsMADS50_KO_and_WT_plants.gif&diff=171439 2014-05-24T00:36:46Z

<p>Fangchao: Photograph was taken when WT plant (W/W) flowered. The OsMADS50 KO (T/T) plant had not bolted.</p> <hr /> <div>Photograph was taken when WT plant (W/W) flowered. The OsMADS50 KO (T/T) plant had not bolted.</div>

Fangchao https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Molecular_network.jpg&diff=171258 2014-05-23T11:41:20Z

<p>Fangchao: The molecular network for florigen gene regulation in rice. OsMADS50 activates Ehd1 expression, and in turn Ehd1 activates RFT1 expression. RFT1 acts as the LD-specific florigen.</p> <hr /> <div>The molecular network for florigen gene regulation in rice.<br /> OsMADS50 activates Ehd1 expression, and in turn Ehd1 activates RFT1 expression. RFT1 acts as the LD-specific florigen.</div>

Fangchao