RiceWiki - User contributions [en]

Os01g0149500

2014-06-08T12:43:36Z

Haidongli: /* References */

Please input one-sentence summary here.

==Annotated Information==
===Function===
The plant genome contains a large number of disease resistance (R) genes that have evolved through diverse mechanisms. Pit confers race-specific resistance against the fungal pathogen Magnaporthe grisea, and is a member of the nucleotide-binding site leucine-rich repeat (NBS-LRR) family of R genes.

===Expression===
The structure of the predicted Pit protein is typical of NBSLRR proteins (Figures 3a, S1 and S3). Pit contains conserved motifs indicative of an NBS domain, and the putative LRR domain (with 18 imperfect repeats) matches the cytoplasmic LRR consensus sequence. The Pit protein encoded by the Nipponbare allele (PitNpb) differs from that encoded by the K59 allele (PitK59) at four amino acid positions: 143 (R fi G), 176 (M fi I), 720 (A fi T) and 780 (M fi V) (Figures 3a, S1 and S3). Two of the substitutions (R143G and M176I) are located in the Nterminal half of the protein, whereas the others are in the LRR domain.
A series of chimeric transgenes are used to examine which substitution(s) is important for determining Pit resistance(Figure 3b). First, the LRR region of PitK59 is replaced with that of PitNpb (A720T/M780V), because a single amino acid difference in the LRR domain has been shown to affect resistance. However, the PitK59 (A720T/M780V) lines conferred resistance (‘R + RM’) to 92% of the transgenic Nipponbare plants, suggesting that the two substitutions in the LRR region are not important for Pit function. Next, mutating the corresponding amino acids in PitNpb (R143G and M176I) to analyze the two substitutions in the N-terminal half of PitK59. A 24% reduction in resistance was observed in the transgenic plants as a result. Whereas the M176I mutation, located between the second CC motif (CC2) and the P loop, conferred resistance to 87% of the plants, R143G, located in CC2, conferred resistance to only 55% of the plants. These results suggest that the R143G mutation affected the resistance. Indeed, transgenic populations harboring the R143G mutation [i.e. PitK59 (R143G/M176I), PitK59 (R143G), Kpro-PitNpb and Ubi-PitNpb] obviously showed higher proportions of RS phenotypes (more than 18%) compared with the other populations. To examine whether these RS phenotypes are caused by the lower expression of chimeric transgenes, RT-PCR analysis was performed (Figure 3c). Both PitK59 (R143G/M176I) and PitK59 (R143G) transgenic plants showed higher levels of transgene expression than K59, irrespective of their phenotypes: i.e. transgenic plants with the RS phenotype also expressed the transgenes at high levels. These results further suggest that the R143G mutation may have destabilized the resistance function. However, considering the result that 80% of gPitNpb plants showed the complete susceptible (S) phenotype (Figure 3b), Pit requires another factor for its activity. As noted above, the structures of PitK59 and PitNpb differ in their promoter and 5¢-UTR regions; moreover, the efficiency of translation is controlled by various features (e.g. 5¢-UTR). Therefore, experiments were taken to examine the effects of the promoter and 5¢-UTR from each gene on Pit function using the constructs Npro5¢-PitK59, Npro-PitK59, Kpro-PitNpb and Ubi-PitNpb (Figure 3b). Npro5¢-PitK59, which contained 1.9 kb of the promoter and the 1.3-kb 5¢-UTR of PitNpb fused to the remainder of PitK59, conferred resistance to only 31% of the T0 plants. Similarly, only 30% of T0 plants expressing Npro-PitK59, in which the 1.9-kb promoter of PitNpb was fused directly with the coding sequence and terminator of PitK59, to eliminate possible effects of the 5¢-UTR of PitNpb, exhibited a resistant phenotype. These results negate the possibility that the extended 5¢-UTR of PitNpb affects Pit function. However, surprisingly, Kpro-PitNpb, which carried the PitNpb coding sequence driven by the PitK59 promoter consisting of the 3¢ fragment of Renovator (1040 bp) and the 5¢-UTR (254 bp) of PitK59, conferred resistance to 78% of the transgenic plants (Figure 3b). Finally, 71% of plants overexpressing the PitNpb open-reading frame (ORF) (Ubi-PitNpb) exhibited resistance (Figure 3b). The expression levels of PitK59 or PitNpb in gPitNpb, Npro5¢-PitK59, Npro-PitK59 and Ubi-PitNpb were correlated with their resistance (Figure 3d,e). These results clearly indicate that the promoter activity conferred by the Renovator retrotransposon fragment, rather than the amino acid substitutions, plays an important role in Pit resistance by enhancing its transcription. Notably, the increased resistance displayed by the plants expressing Kpro-PitNpb, compared with those expressing Npro-PitK59, indicates that promoter activity plays a dominant role in PitK59 function.

===Evolution===
Compared with the non-functional allele PitNpb, the functional allele PitK59 contains four amino acid substitutions, and has the LTR retrotransposon Renovator inserted upstream. Pathogenesis assays using chimeric constructs carrying the various regions of PitK59 and PitNpb suggest that amino acid substitutions might have a potential effect in Pit resistance; more importantly, the upregulated promoter activity conferred by the Renovator sequence is essential for Pit function. It is said that transposon-mediated transcriptional activation may play an important role in the refunctionalization of additional 'sleeping'R genes in the plant genome.

Many R gene clusters are thought to have evolved via tandem and segmental duplications, rearrangement between the genes in a cluster, and point mutations or the deletion of genes (Meyers et al., 2003). As shown in Figure 7, the Pit locus has evolved through a combination of duplication, point mutations and TE insertions. The barley Pit homolog RGA S-reg19 was mapped to chromosome 3H (Madsen et al., 2003). Intriguingly, this position corresponds to the rice Pit locus, and a barley blast resistance QTL (bbr3H) was also mapped near this region
(Chen et al., 2003). This suggests that at least one copy of the ancestral Pit gene emerged before the diversification of the ancestors of rice and barley. Furthermore, although the genomic structure of this homolog(s) is unknown, barley may have a copy of the functional Pit gene with resistance to the blast fungus. After the emergence of the ancestral Pit gene, a duplication event occurred, and the two paralogs probably mutated independently of each other. The overexpression of NBSt1K59 failed to confer resistance (Figure 2a), suggesting that NBSt1K59 lost its function through amino acid substitutions. Amino acid substitutions were detected throughout the protein, not only in the LRR domain but also in the consensus elements of the NBS domain (Figure S1). NBSt1 has an amino acid substitution (C fi F) that is highly conserved in the RNBS-D motif, suggesting one possible cause of the loss of function. Interestingly, the R143G substitution in the CC domain did not occur in NBSt1K59, suggesting that the substitution was generated in Pit after the tandem duplication of the ancestral gene, and is not involved in the disruption of NBSt1K59 function. The ancestral Pit promoter may have been attenuated by mutations before the duplication of the gene, because NBSt1Npb and PitNpb share high similarity in their promoter sequences (data not shown), and both are expressed at low levels in Nipponbare. The point mutations and promoter attenuation would have suppressed Pit resistance to an undetectable level; however, the insertion of Renovator upstream of Pit reactivated its transcription, although we cannot exclude the possibilities of the removal of a repressor sequence or insulation of the promoter against an upstream repressor (Figure 7). The transposition of Renovator in an ancestor of K59 is likely to be a recent event, because the sequences of its 5¢- and 3¢-LTRs are identical.

==Labs working on this gene==
National Agricultural Research Center, National Agriculture and Food Research Organization (NARO), 1-2-1 Inada, Jo-etsu, Niigata 943 0193, Japan

==References==
Please input cited references here.

1.Keiko Hayashi;Hitoshi Yoshida. Refunctionalization of the ancient rice blast disease resistance gene Pit by the recruitment of a retrotransposon as a promoter, The Plant Journal(2009)57,413-425

2.K.Hayashi;N.Yasuda;Y.Fujita;S.Koizumi;H.Yoshida, Identification of the blast resistance gene Pit in rice cultivars using functional markers,Theor Appl Gene(2010)121:1357-1367

==Structured Information==
{{JaponicaGene|
GeneName = Os01g0149500|
Description = Similar to Resistance gene analog PIC23 (Fragment)|
Version = NM_001048560.1 GI:115434533 GeneID:4324511|
Length = 3143 bp|
Definition = Oryza sativa Japonica Group Os01g0149500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:2682222..2685364|
CDS = 2682222..2683988|
GCID = <gbrowseImage1>
name=NC_008394:2682222..2685364
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:2682222..2685364
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgggcacagttttggatgctctcgcttggaaattcttggaaaagctgggtcaacttattgaggatgaggttatcatgacattaagtgtgaaaaggggtattgagagcctgaagaaaaatctggaattcttcaacgctgttcatgaggatgctgaggctctggccatggaggatccagggatagattcatggtggaagaacatgagagatgttatgtttgatgtcgatgacatcgtagatcttttcatggttcattcacagaagctcttgctgccaccaaggccggtatgttgcaatcagcccttgttctctagctttgcaaagttttcatttgatcacatgattgccaaaagaattgataatataaatgaaaagtttgaagaaattaaaatgaacaaagagatgtttggcttggagagaaccaacgggcaacaaatccagataacaattgttgacagaagtcagacatctcctgtagatgaacttgaggtggttggagaagatatcagaagggctattgatgacatcgtcaaaatgattgttagtagtaactacaatgaaagcaggtcaactgtttttgggatccaggggatgggaggcatcggtaagacaacattagctcaaaagatatacaatgaacagaggataagggagaaattccaagttcatatatggttgtgcatttctcagaattacacagagaccagtttgttaaaacaggcaatcagaatggctggaggaatttgtgatcagctggagactaagactgagcttctaccgcttctagtggacactattagagggaagagtgtttttcttgtgctggatgatgtttggaaatctgatgtatggattgatcttcttcgtttgcctttcttgagaggtttgaattctcatatccttgtgacctcaagaaacctggatgttttggttgaaatgcatgcaacttatacccacaaggtaaacaaaatgaatgattgcgatggcctagaactgctgatgaagatgtcattagggccatacgagcaaagcagagaattcagtggtgttggatatcaaattgtcaaaaagtgtgatggccttcctcttgccatcaaagttgttgcaggtgttctatctactaaaagaacaagggcagaatgggagagtatccgagatagcaagtggtccattcatggacttcccagagaactaggaggccctctgtacttaagctacagcaacttaccccctgaacttaaacaatgctttctctggtgtgctttgttgccgtcaaattttgtgatacgtcgtgatgctgtcgcttactggtgggttgctgaaggtttcgtgacggaagtgcatgggtactcaatacatgaggttgctgaagagtactaccatgagctaatcagaaggaatcttctgcagccacgaccagagtttgtagacaaaggcgagtcaacaatgcatgatctattgaggtcacttggtcaatttttgacaaaggatcactccatattcatgaacatggaatatagcaaagccttaccaaatctacgccacttatgtatcagcaatgatgtagaagaaatacctgctatagaaaagcaaaagtgcttgaggagcctcctagtttttgataacaaaaacttcatgaaaattaataaggacattttcagagaactcaagcatatccgtgtcttagttctcagtggaacaagcatccaaatcataccagagtcagtgggaaacttcttgttactgaggcttctagatctaagctat</cdnaseq>|
AA = <aaseq>MGTVLDALAWKFLEKLGQLIEDEVIMTLSVKRGIESLKKNLEFF NAVHEDAEALAMEDPGIDSWWKNMRDVMFDVDDIVDLFMVHSQKLLLPPRPVCCNQPL FSSFAKFSFDHMIAKRIDNINEKFEEIKMNKEMFGLERTNGQQIQITIVDRSQTSPVD ELEVVGEDIRRAIDDIVKMIVSSNYNESRSTVFGIQGMGGIGKTTLAQKIYNEQRIRE KFQVHIWLCISQNYTETSLLKQAIRMAGGICDQLETKTELLPLLVDTIRGKSVFLVLD DVWKSDVWIDLLRLPFLRGLNSHILVTSRNLDVLVEMHATYTHKVNKMNDCDGLELLM KMSLGPYEQSREFSGVGYQIVKKCDGLPLAIKVVAGVLSTKRTRAEWESIRDSKWSIH GLPRELGGPLYLSYSNLPPELKQCFLWCALLPSNFVIRRDAVAYWWVAEGFVTEVHGY SIHEVAEEYYHELIRRNLLQPRPEFVDKGESTMHDLLRSLGQFLTKDHSIFMNMEYSK ALPNLRHLCISNDVEEIPAIEKQKCLRSLLVFDNKNFMKINKDIFRELKHIRVLVLSG TSIQIIPESVGNFLLLRLLDLSY</aaseq>|
DNA = <dnaseqindica>1377..3143#gtgcggtggggggcatttcatcgaacaccattcgcgcatcgctctccccgagcgaaccgcattcgttacgttacgttacgtgcgagatgaatcaacttgtcaggtacaaacacaaccggagaatttgcttttgtttatactggagttatacgatgtgatcctgatccaggctcagttcgacctagcccctgcttatctttgcttgtgggtcgttgtcgccgtctttggttacgctaggagaagcatctctctttcttgcgtaggtaatcaactatcttaatttttcttttgtgtcttgattatgtgcaacaccgagtgatgcgaatgggatgtgttcctttcgatgttttttctatataagctctgaaacttggacttttgcaacaaatggttcgggtgcagatgagctgtttgacgaaatgcccaggagattcctgcgtgcgttgcatgcccagcggcggtttgactctatttttcctttagtatggtgtctgctctctggtgatctaatgtaccattttctcttaaccttgttttacccaatcttaatgcaaagatgcataaagagaactctgcatattcctgttttctcttaaccttgtttcagagaaccttgttttctctgaactctgcaaaagattttctgcatattcctgaaaaaaggagaactctgctccggtgcttttaactagacttataaaggctttggcataggattcctgtattggttgatgcaaaatctgctggtaacagtcagctaaattcgcttaaaaagtacacttctttgggtcttcttgctagtgcaggtagagaagcaccatggccttcctggtttcttaggtggtaaacaggcaaagaaagtaaagcaccaagtggcgccattgctgcaatgaaatcaaattattattagcaagtggatgaaaggaaaagaatacacagcctggaagagctttcaacttgaatccccttgcaattgtgtaacactacttgtccttttcacattgcatcatcgttgaaacatttggattttaatacaaaaatcattgtgggtagattttgcctcaaaaagtgctttcatactgttttaattttgtcaggtttttcagacgttttgcaatagagaatgtcaatgtccattgtgacaaaggcagttggtggcaagaaagggaggagaatctataacagctctcttctgcaatctgcattgccctctctgttctcatcaggttttagccacactgcttcaatatctctttccatcttccgtatttaagtgatcggcactcagcagctctgtgagatatctcttctgggatctgcagtttcaagcatctcccataggagagatccagtgggaatccgtaggttctagcactatgggcacagttttggatgctctcgcttggaaattcttggaaaagctgggtcaacttattgaggatgaggttatcatgacattaagtgtgaaaaggggtattgagagcctgaagaaaaatctggaattcttcaacgctgttcatgaggatgctgaggctctggccatggaggatccagggatagattcatggtggaagaacatgagagatgttatgtttgatgtcgatgacatcgtagatcttttcatggttcattcacagaagctcttgctgccaccaaggccggtatgttgcaatcagcccttgttctctagctttgcaaagttttcatttgatcacatgattgccaaaagaattgataatataaatgaaaagtttgaagaaattaaaatgaacaaagagatgtttggcttggagagaaccaacgggcaacaaatccagataacaattgttgacagaagtcagacatctcctgtagatgaacttgaggtggttggagaagatatcagaagggctattgatgacatcgtcaaaatgattgttagtagtaactacaatgaaagcaggtcaactgtttttgggatccaggggatgggaggcatcggtaagacaacattagctcaaaagatatacaatgaacagaggataagggagaaattccaagttcatatatggttgtgcatttctcagaattacacagagaccagtttgttaaaacaggcaatcagaatggctggaggaatttgtgatcagctggagactaagactgagcttctaccgcttctagtggacactattagagggaagagtgtttttcttgtgctggatgatgtttggaaatctgatgtatggattgatcttcttcgtttgcctttcttgagaggtttgaattctcatatccttgtgacctcaagaaacctggatgttttggttgaaatgcatgcaacttatacccacaaggtaaacaaaatgaatgattgcgatggcctagaactgctgatgaagatgtcattagggccatacgagcaaagcagagaattcagtggtgttggatatcaaattgtcaaaaagtgtgatggccttcctcttgccatcaaagttgttgcaggtgttctatctactaaaagaacaagggcagaatgggagagtatccgagatagcaagtggtccattcatggacttcccagagaactaggaggccctctgtacttaagctacagcaacttaccccctgaacttaaacaatgctttctctggtgtgctttgttgccgtcaaattttgtgatacgtcgtgatgctgtcgcttactggtgggttgctgaaggtttcgtgacggaagtgcatgggtactcaatacatgaggttgctgaagagtactaccatgagctaatcagaaggaatcttctgcagccacgaccagagtttgtagacaaaggcgagtcaacaatgcatgatctattgaggtcacttggtcaatttttgacaaaggatcactccatattcatgaacatggaatatagcaaagccttaccaaatctacgccacttatgtatcagcaatgatgtagaagaaatacctgctatagaaaagcaaaagtgcttgaggagcctcctagtttttgataacaaaaacttcatgaaaattaataaggacattttcagagaactcaagcatatccgtgtcttagttctcagtggaacaagcatccaaatcataccagagtcagtgggaaacttcttgttactgaggcttctagatctaagctat</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001048560.1 RefSeq:Os01g0149500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

Os01g0149500

2014-05-27T16:08:38Z

Haidongli: /* Evolution */

Please input one-sentence summary here.

==Annotated Information==
===Function===
The plant genome contains a large number of disease resistance (R) genes that have evolved through diverse mechanisms. Pit confers race-specific resistance against the fungal pathogen Magnaporthe grisea, and is a member of the nucleotide-binding site leucine-rich repeat (NBS-LRR) family of R genes.

===Expression===
The structure of the predicted Pit protein is typical of NBSLRR proteins (Figures 3a, S1 and S3). Pit contains conserved motifs indicative of an NBS domain, and the putative LRR domain (with 18 imperfect repeats) matches the cytoplasmic LRR consensus sequence. The Pit protein encoded by the Nipponbare allele (PitNpb) differs from that encoded by the K59 allele (PitK59) at four amino acid positions: 143 (R fi G), 176 (M fi I), 720 (A fi T) and 780 (M fi V) (Figures 3a, S1 and S3). Two of the substitutions (R143G and M176I) are located in the Nterminal half of the protein, whereas the others are in the LRR domain.
A series of chimeric transgenes are used to examine which substitution(s) is important for determining Pit resistance(Figure 3b). First, the LRR region of PitK59 is replaced with that of PitNpb (A720T/M780V), because a single amino acid difference in the LRR domain has been shown to affect resistance. However, the PitK59 (A720T/M780V) lines conferred resistance (‘R + RM’) to 92% of the transgenic Nipponbare plants, suggesting that the two substitutions in the LRR region are not important for Pit function. Next, mutating the corresponding amino acids in PitNpb (R143G and M176I) to analyze the two substitutions in the N-terminal half of PitK59. A 24% reduction in resistance was observed in the transgenic plants as a result. Whereas the M176I mutation, located between the second CC motif (CC2) and the P loop, conferred resistance to 87% of the plants, R143G, located in CC2, conferred resistance to only 55% of the plants. These results suggest that the R143G mutation affected the resistance. Indeed, transgenic populations harboring the R143G mutation [i.e. PitK59 (R143G/M176I), PitK59 (R143G), Kpro-PitNpb and Ubi-PitNpb] obviously showed higher proportions of RS phenotypes (more than 18%) compared with the other populations. To examine whether these RS phenotypes are caused by the lower expression of chimeric transgenes, RT-PCR analysis was performed (Figure 3c). Both PitK59 (R143G/M176I) and PitK59 (R143G) transgenic plants showed higher levels of transgene expression than K59, irrespective of their phenotypes: i.e. transgenic plants with the RS phenotype also expressed the transgenes at high levels. These results further suggest that the R143G mutation may have destabilized the resistance function. However, considering the result that 80% of gPitNpb plants showed the complete susceptible (S) phenotype (Figure 3b), Pit requires another factor for its activity. As noted above, the structures of PitK59 and PitNpb differ in their promoter and 5¢-UTR regions; moreover, the efficiency of translation is controlled by various features (e.g. 5¢-UTR). Therefore, experiments were taken to examine the effects of the promoter and 5¢-UTR from each gene on Pit function using the constructs Npro5¢-PitK59, Npro-PitK59, Kpro-PitNpb and Ubi-PitNpb (Figure 3b). Npro5¢-PitK59, which contained 1.9 kb of the promoter and the 1.3-kb 5¢-UTR of PitNpb fused to the remainder of PitK59, conferred resistance to only 31% of the T0 plants. Similarly, only 30% of T0 plants expressing Npro-PitK59, in which the 1.9-kb promoter of PitNpb was fused directly with the coding sequence and terminator of PitK59, to eliminate possible effects of the 5¢-UTR of PitNpb, exhibited a resistant phenotype. These results negate the possibility that the extended 5¢-UTR of PitNpb affects Pit function. However, surprisingly, Kpro-PitNpb, which carried the PitNpb coding sequence driven by the PitK59 promoter consisting of the 3¢ fragment of Renovator (1040 bp) and the 5¢-UTR (254 bp) of PitK59, conferred resistance to 78% of the transgenic plants (Figure 3b). Finally, 71% of plants overexpressing the PitNpb open-reading frame (ORF) (Ubi-PitNpb) exhibited resistance (Figure 3b). The expression levels of PitK59 or PitNpb in gPitNpb, Npro5¢-PitK59, Npro-PitK59 and Ubi-PitNpb were correlated with their resistance (Figure 3d,e). These results clearly indicate that the promoter activity conferred by the Renovator retrotransposon fragment, rather than the amino acid substitutions, plays an important role in Pit resistance by enhancing its transcription. Notably, the increased resistance displayed by the plants expressing Kpro-PitNpb, compared with those expressing Npro-PitK59, indicates that promoter activity plays a dominant role in PitK59 function.

===Evolution===
Compared with the non-functional allele PitNpb, the functional allele PitK59 contains four amino acid substitutions, and has the LTR retrotransposon Renovator inserted upstream. Pathogenesis assays using chimeric constructs carrying the various regions of PitK59 and PitNpb suggest that amino acid substitutions might have a potential effect in Pit resistance; more importantly, the upregulated promoter activity conferred by the Renovator sequence is essential for Pit function. It is said that transposon-mediated transcriptional activation may play an important role in the refunctionalization of additional 'sleeping'R genes in the plant genome.

Many R gene clusters are thought to have evolved via tandem and segmental duplications, rearrangement between the genes in a cluster, and point mutations or the deletion of genes (Meyers et al., 2003). As shown in Figure 7, the Pit locus has evolved through a combination of duplication, point mutations and TE insertions. The barley Pit homolog RGA S-reg19 was mapped to chromosome 3H (Madsen et al., 2003). Intriguingly, this position corresponds to the rice Pit locus, and a barley blast resistance QTL (bbr3H) was also mapped near this region
(Chen et al., 2003). This suggests that at least one copy of the ancestral Pit gene emerged before the diversification of the ancestors of rice and barley. Furthermore, although the genomic structure of this homolog(s) is unknown, barley may have a copy of the functional Pit gene with resistance to the blast fungus. After the emergence of the ancestral Pit gene, a duplication event occurred, and the two paralogs probably mutated independently of each other. The overexpression of NBSt1K59 failed to confer resistance (Figure 2a), suggesting that NBSt1K59 lost its function through amino acid substitutions. Amino acid substitutions were detected throughout the protein, not only in the LRR domain but also in the consensus elements of the NBS domain (Figure S1). NBSt1 has an amino acid substitution (C fi F) that is highly conserved in the RNBS-D motif, suggesting one possible cause of the loss of function. Interestingly, the R143G substitution in the CC domain did not occur in NBSt1K59, suggesting that the substitution was generated in Pit after the tandem duplication of the ancestral gene, and is not involved in the disruption of NBSt1K59 function. The ancestral Pit promoter may have been attenuated by mutations before the duplication of the gene, because NBSt1Npb and PitNpb share high similarity in their promoter sequences (data not shown), and both are expressed at low levels in Nipponbare. The point mutations and promoter attenuation would have suppressed Pit resistance to an undetectable level; however, the insertion of Renovator upstream of Pit reactivated its transcription, although we cannot exclude the possibilities of the removal of a repressor sequence or insulation of the promoter against an upstream repressor (Figure 7). The transposition of Renovator in an ancestor of K59 is likely to be a recent event, because the sequences of its 5¢- and 3¢-LTRs are identical.

==Labs working on this gene==
National Agricultural Research Center, National Agriculture and Food Research Organization (NARO), 1-2-1 Inada, Jo-etsu, Niigata 943 0193, Japan

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os01g0149500|
Description = Similar to Resistance gene analog PIC23 (Fragment)|
Version = NM_001048560.1 GI:115434533 GeneID:4324511|
Length = 3143 bp|
Definition = Oryza sativa Japonica Group Os01g0149500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:2682222..2685364|
CDS = 2682222..2683988|
GCID = <gbrowseImage1>
name=NC_008394:2682222..2685364
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:2682222..2685364
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgggcacagttttggatgctctcgcttggaaattcttggaaaagctgggtcaacttattgaggatgaggttatcatgacattaagtgtgaaaaggggtattgagagcctgaagaaaaatctggaattcttcaacgctgttcatgaggatgctgaggctctggccatggaggatccagggatagattcatggtggaagaacatgagagatgttatgtttgatgtcgatgacatcgtagatcttttcatggttcattcacagaagctcttgctgccaccaaggccggtatgttgcaatcagcccttgttctctagctttgcaaagttttcatttgatcacatgattgccaaaagaattgataatataaatgaaaagtttgaagaaattaaaatgaacaaagagatgtttggcttggagagaaccaacgggcaacaaatccagataacaattgttgacagaagtcagacatctcctgtagatgaacttgaggtggttggagaagatatcagaagggctattgatgacatcgtcaaaatgattgttagtagtaactacaatgaaagcaggtcaactgtttttgggatccaggggatgggaggcatcggtaagacaacattagctcaaaagatatacaatgaacagaggataagggagaaattccaagttcatatatggttgtgcatttctcagaattacacagagaccagtttgttaaaacaggcaatcagaatggctggaggaatttgtgatcagctggagactaagactgagcttctaccgcttctagtggacactattagagggaagagtgtttttcttgtgctggatgatgtttggaaatctgatgtatggattgatcttcttcgtttgcctttcttgagaggtttgaattctcatatccttgtgacctcaagaaacctggatgttttggttgaaatgcatgcaacttatacccacaaggtaaacaaaatgaatgattgcgatggcctagaactgctgatgaagatgtcattagggccatacgagcaaagcagagaattcagtggtgttggatatcaaattgtcaaaaagtgtgatggccttcctcttgccatcaaagttgttgcaggtgttctatctactaaaagaacaagggcagaatgggagagtatccgagatagcaagtggtccattcatggacttcccagagaactaggaggccctctgtacttaagctacagcaacttaccccctgaacttaaacaatgctttctctggtgtgctttgttgccgtcaaattttgtgatacgtcgtgatgctgtcgcttactggtgggttgctgaaggtttcgtgacggaagtgcatgggtactcaatacatgaggttgctgaagagtactaccatgagctaatcagaaggaatcttctgcagccacgaccagagtttgtagacaaaggcgagtcaacaatgcatgatctattgaggtcacttggtcaatttttgacaaaggatcactccatattcatgaacatggaatatagcaaagccttaccaaatctacgccacttatgtatcagcaatgatgtagaagaaatacctgctatagaaaagcaaaagtgcttgaggagcctcctagtttttgataacaaaaacttcatgaaaattaataaggacattttcagagaactcaagcatatccgtgtcttagttctcagtggaacaagcatccaaatcataccagagtcagtgggaaacttcttgttactgaggcttctagatctaagctat</cdnaseq>|
AA = <aaseq>MGTVLDALAWKFLEKLGQLIEDEVIMTLSVKRGIESLKKNLEFF NAVHEDAEALAMEDPGIDSWWKNMRDVMFDVDDIVDLFMVHSQKLLLPPRPVCCNQPL FSSFAKFSFDHMIAKRIDNINEKFEEIKMNKEMFGLERTNGQQIQITIVDRSQTSPVD ELEVVGEDIRRAIDDIVKMIVSSNYNESRSTVFGIQGMGGIGKTTLAQKIYNEQRIRE KFQVHIWLCISQNYTETSLLKQAIRMAGGICDQLETKTELLPLLVDTIRGKSVFLVLD DVWKSDVWIDLLRLPFLRGLNSHILVTSRNLDVLVEMHATYTHKVNKMNDCDGLELLM KMSLGPYEQSREFSGVGYQIVKKCDGLPLAIKVVAGVLSTKRTRAEWESIRDSKWSIH GLPRELGGPLYLSYSNLPPELKQCFLWCALLPSNFVIRRDAVAYWWVAEGFVTEVHGY SIHEVAEEYYHELIRRNLLQPRPEFVDKGESTMHDLLRSLGQFLTKDHSIFMNMEYSK ALPNLRHLCISNDVEEIPAIEKQKCLRSLLVFDNKNFMKINKDIFRELKHIRVLVLSG TSIQIIPESVGNFLLLRLLDLSY</aaseq>|
DNA = <dnaseqindica>1377..3143#gtgcggtggggggcatttcatcgaacaccattcgcgcatcgctctccccgagcgaaccgcattcgttacgttacgttacgtgcgagatgaatcaacttgtcaggtacaaacacaaccggagaatttgcttttgtttatactggagttatacgatgtgatcctgatccaggctcagttcgacctagcccctgcttatctttgcttgtgggtcgttgtcgccgtctttggttacgctaggagaagcatctctctttcttgcgtaggtaatcaactatcttaatttttcttttgtgtcttgattatgtgcaacaccgagtgatgcgaatgggatgtgttcctttcgatgttttttctatataagctctgaaacttggacttttgcaacaaatggttcgggtgcagatgagctgtttgacgaaatgcccaggagattcctgcgtgcgttgcatgcccagcggcggtttgactctatttttcctttagtatggtgtctgctctctggtgatctaatgtaccattttctcttaaccttgttttacccaatcttaatgcaaagatgcataaagagaactctgcatattcctgttttctcttaaccttgtttcagagaaccttgttttctctgaactctgcaaaagattttctgcatattcctgaaaaaaggagaactctgctccggtgcttttaactagacttataaaggctttggcataggattcctgtattggttgatgcaaaatctgctggtaacagtcagctaaattcgcttaaaaagtacacttctttgggtcttcttgctagtgcaggtagagaagcaccatggccttcctggtttcttaggtggtaaacaggcaaagaaagtaaagcaccaagtggcgccattgctgcaatgaaatcaaattattattagcaagtggatgaaaggaaaagaatacacagcctggaagagctttcaacttgaatccccttgcaattgtgtaacactacttgtccttttcacattgcatcatcgttgaaacatttggattttaatacaaaaatcattgtgggtagattttgcctcaaaaagtgctttcatactgttttaattttgtcaggtttttcagacgttttgcaatagagaatgtcaatgtccattgtgacaaaggcagttggtggcaagaaagggaggagaatctataacagctctcttctgcaatctgcattgccctctctgttctcatcaggttttagccacactgcttcaatatctctttccatcttccgtatttaagtgatcggcactcagcagctctgtgagatatctcttctgggatctgcagtttcaagcatctcccataggagagatccagtgggaatccgtaggttctagcactatgggcacagttttggatgctctcgcttggaaattcttggaaaagctgggtcaacttattgaggatgaggttatcatgacattaagtgtgaaaaggggtattgagagcctgaagaaaaatctggaattcttcaacgctgttcatgaggatgctgaggctctggccatggaggatccagggatagattcatggtggaagaacatgagagatgttatgtttgatgtcgatgacatcgtagatcttttcatggttcattcacagaagctcttgctgccaccaaggccggtatgttgcaatcagcccttgttctctagctttgcaaagttttcatttgatcacatgattgccaaaagaattgataatataaatgaaaagtttgaagaaattaaaatgaacaaagagatgtttggcttggagagaaccaacgggcaacaaatccagataacaattgttgacagaagtcagacatctcctgtagatgaacttgaggtggttggagaagatatcagaagggctattgatgacatcgtcaaaatgattgttagtagtaactacaatgaaagcaggtcaactgtttttgggatccaggggatgggaggcatcggtaagacaacattagctcaaaagatatacaatgaacagaggataagggagaaattccaagttcatatatggttgtgcatttctcagaattacacagagaccagtttgttaaaacaggcaatcagaatggctggaggaatttgtgatcagctggagactaagactgagcttctaccgcttctagtggacactattagagggaagagtgtttttcttgtgctggatgatgtttggaaatctgatgtatggattgatcttcttcgtttgcctttcttgagaggtttgaattctcatatccttgtgacctcaagaaacctggatgttttggttgaaatgcatgcaacttatacccacaaggtaaacaaaatgaatgattgcgatggcctagaactgctgatgaagatgtcattagggccatacgagcaaagcagagaattcagtggtgttggatatcaaattgtcaaaaagtgtgatggccttcctcttgccatcaaagttgttgcaggtgttctatctactaaaagaacaagggcagaatgggagagtatccgagatagcaagtggtccattcatggacttcccagagaactaggaggccctctgtacttaagctacagcaacttaccccctgaacttaaacaatgctttctctggtgtgctttgttgccgtcaaattttgtgatacgtcgtgatgctgtcgcttactggtgggttgctgaaggtttcgtgacggaagtgcatgggtactcaatacatgaggttgctgaagagtactaccatgagctaatcagaaggaatcttctgcagccacgaccagagtttgtagacaaaggcgagtcaacaatgcatgatctattgaggtcacttggtcaatttttgacaaaggatcactccatattcatgaacatggaatatagcaaagccttaccaaatctacgccacttatgtatcagcaatgatgtagaagaaatacctgctatagaaaagcaaaagtgcttgaggagcctcctagtttttgataacaaaaacttcatgaaaattaataaggacattttcagagaactcaagcatatccgtgtcttagttctcagtggaacaagcatccaaatcataccagagtcagtgggaaacttcttgttactgaggcttctagatctaagctat</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001048560.1 RefSeq:Os01g0149500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

Os01g0149500

2014-05-27T16:03:17Z

Haidongli: /* Expression */

Please input one-sentence summary here.

==Annotated Information==
===Function===
The plant genome contains a large number of disease resistance (R) genes that have evolved through diverse mechanisms. Pit confers race-specific resistance against the fungal pathogen Magnaporthe grisea, and is a member of the nucleotide-binding site leucine-rich repeat (NBS-LRR) family of R genes.

===Expression===
The structure of the predicted Pit protein is typical of NBSLRR proteins (Figures 3a, S1 and S3). Pit contains conserved motifs indicative of an NBS domain, and the putative LRR domain (with 18 imperfect repeats) matches the cytoplasmic LRR consensus sequence. The Pit protein encoded by the Nipponbare allele (PitNpb) differs from that encoded by the K59 allele (PitK59) at four amino acid positions: 143 (R fi G), 176 (M fi I), 720 (A fi T) and 780 (M fi V) (Figures 3a, S1 and S3). Two of the substitutions (R143G and M176I) are located in the Nterminal half of the protein, whereas the others are in the LRR domain.
A series of chimeric transgenes are used to examine which substitution(s) is important for determining Pit resistance(Figure 3b). First, the LRR region of PitK59 is replaced with that of PitNpb (A720T/M780V), because a single amino acid difference in the LRR domain has been shown to affect resistance. However, the PitK59 (A720T/M780V) lines conferred resistance (‘R + RM’) to 92% of the transgenic Nipponbare plants, suggesting that the two substitutions in the LRR region are not important for Pit function. Next, mutating the corresponding amino acids in PitNpb (R143G and M176I) to analyze the two substitutions in the N-terminal half of PitK59. A 24% reduction in resistance was observed in the transgenic plants as a result. Whereas the M176I mutation, located between the second CC motif (CC2) and the P loop, conferred resistance to 87% of the plants, R143G, located in CC2, conferred resistance to only 55% of the plants. These results suggest that the R143G mutation affected the resistance. Indeed, transgenic populations harboring the R143G mutation [i.e. PitK59 (R143G/M176I), PitK59 (R143G), Kpro-PitNpb and Ubi-PitNpb] obviously showed higher proportions of RS phenotypes (more than 18%) compared with the other populations. To examine whether these RS phenotypes are caused by the lower expression of chimeric transgenes, RT-PCR analysis was performed (Figure 3c). Both PitK59 (R143G/M176I) and PitK59 (R143G) transgenic plants showed higher levels of transgene expression than K59, irrespective of their phenotypes: i.e. transgenic plants with the RS phenotype also expressed the transgenes at high levels. These results further suggest that the R143G mutation may have destabilized the resistance function. However, considering the result that 80% of gPitNpb plants showed the complete susceptible (S) phenotype (Figure 3b), Pit requires another factor for its activity. As noted above, the structures of PitK59 and PitNpb differ in their promoter and 5¢-UTR regions; moreover, the efficiency of translation is controlled by various features (e.g. 5¢-UTR). Therefore, experiments were taken to examine the effects of the promoter and 5¢-UTR from each gene on Pit function using the constructs Npro5¢-PitK59, Npro-PitK59, Kpro-PitNpb and Ubi-PitNpb (Figure 3b). Npro5¢-PitK59, which contained 1.9 kb of the promoter and the 1.3-kb 5¢-UTR of PitNpb fused to the remainder of PitK59, conferred resistance to only 31% of the T0 plants. Similarly, only 30% of T0 plants expressing Npro-PitK59, in which the 1.9-kb promoter of PitNpb was fused directly with the coding sequence and terminator of PitK59, to eliminate possible effects of the 5¢-UTR of PitNpb, exhibited a resistant phenotype. These results negate the possibility that the extended 5¢-UTR of PitNpb affects Pit function. However, surprisingly, Kpro-PitNpb, which carried the PitNpb coding sequence driven by the PitK59 promoter consisting of the 3¢ fragment of Renovator (1040 bp) and the 5¢-UTR (254 bp) of PitK59, conferred resistance to 78% of the transgenic plants (Figure 3b). Finally, 71% of plants overexpressing the PitNpb open-reading frame (ORF) (Ubi-PitNpb) exhibited resistance (Figure 3b). The expression levels of PitK59 or PitNpb in gPitNpb, Npro5¢-PitK59, Npro-PitK59 and Ubi-PitNpb were correlated with their resistance (Figure 3d,e). These results clearly indicate that the promoter activity conferred by the Renovator retrotransposon fragment, rather than the amino acid substitutions, plays an important role in Pit resistance by enhancing its transcription. Notably, the increased resistance displayed by the plants expressing Kpro-PitNpb, compared with those expressing Npro-PitK59, indicates that promoter activity plays a dominant role in PitK59 function.

===Evolution===
Compared with the non-functional allele PitNpb, the functional allele PitK59 contains four amino acid substitutions, and has the LTR retrotransposon Renovator inserted upstream. Pathogenesis assays using chimeric constructs carrying the various regions of PitK59 and PitNpb suggest that amino acid substitutions might have a potential effect in Pit resistance; more importantly, the upregulated promoter activity conferred by the Renovator sequence is essential for Pit function. It is said that transposon-mediated transcriptional activation may play an important role in the refunctionalization of additional 'sleeping'R genes in the plant genome.

==Labs working on this gene==
National Agricultural Research Center, National Agriculture and Food Research Organization (NARO), 1-2-1 Inada, Jo-etsu, Niigata 943 0193, Japan

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os01g0149500|
Description = Similar to Resistance gene analog PIC23 (Fragment)|
Version = NM_001048560.1 GI:115434533 GeneID:4324511|
Length = 3143 bp|
Definition = Oryza sativa Japonica Group Os01g0149500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:2682222..2685364|
CDS = 2682222..2683988|
GCID = <gbrowseImage1>
name=NC_008394:2682222..2685364
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:2682222..2685364
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgggcacagttttggatgctctcgcttggaaattcttggaaaagctgggtcaacttattgaggatgaggttatcatgacattaagtgtgaaaaggggtattgagagcctgaagaaaaatctggaattcttcaacgctgttcatgaggatgctgaggctctggccatggaggatccagggatagattcatggtggaagaacatgagagatgttatgtttgatgtcgatgacatcgtagatcttttcatggttcattcacagaagctcttgctgccaccaaggccggtatgttgcaatcagcccttgttctctagctttgcaaagttttcatttgatcacatgattgccaaaagaattgataatataaatgaaaagtttgaagaaattaaaatgaacaaagagatgtttggcttggagagaaccaacgggcaacaaatccagataacaattgttgacagaagtcagacatctcctgtagatgaacttgaggtggttggagaagatatcagaagggctattgatgacatcgtcaaaatgattgttagtagtaactacaatgaaagcaggtcaactgtttttgggatccaggggatgggaggcatcggtaagacaacattagctcaaaagatatacaatgaacagaggataagggagaaattccaagttcatatatggttgtgcatttctcagaattacacagagaccagtttgttaaaacaggcaatcagaatggctggaggaatttgtgatcagctggagactaagactgagcttctaccgcttctagtggacactattagagggaagagtgtttttcttgtgctggatgatgtttggaaatctgatgtatggattgatcttcttcgtttgcctttcttgagaggtttgaattctcatatccttgtgacctcaagaaacctggatgttttggttgaaatgcatgcaacttatacccacaaggtaaacaaaatgaatgattgcgatggcctagaactgctgatgaagatgtcattagggccatacgagcaaagcagagaattcagtggtgttggatatcaaattgtcaaaaagtgtgatggccttcctcttgccatcaaagttgttgcaggtgttctatctactaaaagaacaagggcagaatgggagagtatccgagatagcaagtggtccattcatggacttcccagagaactaggaggccctctgtacttaagctacagcaacttaccccctgaacttaaacaatgctttctctggtgtgctttgttgccgtcaaattttgtgatacgtcgtgatgctgtcgcttactggtgggttgctgaaggtttcgtgacggaagtgcatgggtactcaatacatgaggttgctgaagagtactaccatgagctaatcagaaggaatcttctgcagccacgaccagagtttgtagacaaaggcgagtcaacaatgcatgatctattgaggtcacttggtcaatttttgacaaaggatcactccatattcatgaacatggaatatagcaaagccttaccaaatctacgccacttatgtatcagcaatgatgtagaagaaatacctgctatagaaaagcaaaagtgcttgaggagcctcctagtttttgataacaaaaacttcatgaaaattaataaggacattttcagagaactcaagcatatccgtgtcttagttctcagtggaacaagcatccaaatcataccagagtcagtgggaaacttcttgttactgaggcttctagatctaagctat</cdnaseq>|
AA = <aaseq>MGTVLDALAWKFLEKLGQLIEDEVIMTLSVKRGIESLKKNLEFF NAVHEDAEALAMEDPGIDSWWKNMRDVMFDVDDIVDLFMVHSQKLLLPPRPVCCNQPL FSSFAKFSFDHMIAKRIDNINEKFEEIKMNKEMFGLERTNGQQIQITIVDRSQTSPVD ELEVVGEDIRRAIDDIVKMIVSSNYNESRSTVFGIQGMGGIGKTTLAQKIYNEQRIRE KFQVHIWLCISQNYTETSLLKQAIRMAGGICDQLETKTELLPLLVDTIRGKSVFLVLD DVWKSDVWIDLLRLPFLRGLNSHILVTSRNLDVLVEMHATYTHKVNKMNDCDGLELLM KMSLGPYEQSREFSGVGYQIVKKCDGLPLAIKVVAGVLSTKRTRAEWESIRDSKWSIH GLPRELGGPLYLSYSNLPPELKQCFLWCALLPSNFVIRRDAVAYWWVAEGFVTEVHGY SIHEVAEEYYHELIRRNLLQPRPEFVDKGESTMHDLLRSLGQFLTKDHSIFMNMEYSK ALPNLRHLCISNDVEEIPAIEKQKCLRSLLVFDNKNFMKINKDIFRELKHIRVLVLSG TSIQIIPESVGNFLLLRLLDLSY</aaseq>|
DNA = <dnaseqindica>1377..3143#gtgcggtggggggcatttcatcgaacaccattcgcgcatcgctctccccgagcgaaccgcattcgttacgttacgttacgtgcgagatgaatcaacttgtcaggtacaaacacaaccggagaatttgcttttgtttatactggagttatacgatgtgatcctgatccaggctcagttcgacctagcccctgcttatctttgcttgtgggtcgttgtcgccgtctttggttacgctaggagaagcatctctctttcttgcgtaggtaatcaactatcttaatttttcttttgtgtcttgattatgtgcaacaccgagtgatgcgaatgggatgtgttcctttcgatgttttttctatataagctctgaaacttggacttttgcaacaaatggttcgggtgcagatgagctgtttgacgaaatgcccaggagattcctgcgtgcgttgcatgcccagcggcggtttgactctatttttcctttagtatggtgtctgctctctggtgatctaatgtaccattttctcttaaccttgttttacccaatcttaatgcaaagatgcataaagagaactctgcatattcctgttttctcttaaccttgtttcagagaaccttgttttctctgaactctgcaaaagattttctgcatattcctgaaaaaaggagaactctgctccggtgcttttaactagacttataaaggctttggcataggattcctgtattggttgatgcaaaatctgctggtaacagtcagctaaattcgcttaaaaagtacacttctttgggtcttcttgctagtgcaggtagagaagcaccatggccttcctggtttcttaggtggtaaacaggcaaagaaagtaaagcaccaagtggcgccattgctgcaatgaaatcaaattattattagcaagtggatgaaaggaaaagaatacacagcctggaagagctttcaacttgaatccccttgcaattgtgtaacactacttgtccttttcacattgcatcatcgttgaaacatttggattttaatacaaaaatcattgtgggtagattttgcctcaaaaagtgctttcatactgttttaattttgtcaggtttttcagacgttttgcaatagagaatgtcaatgtccattgtgacaaaggcagttggtggcaagaaagggaggagaatctataacagctctcttctgcaatctgcattgccctctctgttctcatcaggttttagccacactgcttcaatatctctttccatcttccgtatttaagtgatcggcactcagcagctctgtgagatatctcttctgggatctgcagtttcaagcatctcccataggagagatccagtgggaatccgtaggttctagcactatgggcacagttttggatgctctcgcttggaaattcttggaaaagctgggtcaacttattgaggatgaggttatcatgacattaagtgtgaaaaggggtattgagagcctgaagaaaaatctggaattcttcaacgctgttcatgaggatgctgaggctctggccatggaggatccagggatagattcatggtggaagaacatgagagatgttatgtttgatgtcgatgacatcgtagatcttttcatggttcattcacagaagctcttgctgccaccaaggccggtatgttgcaatcagcccttgttctctagctttgcaaagttttcatttgatcacatgattgccaaaagaattgataatataaatgaaaagtttgaagaaattaaaatgaacaaagagatgtttggcttggagagaaccaacgggcaacaaatccagataacaattgttgacagaagtcagacatctcctgtagatgaacttgaggtggttggagaagatatcagaagggctattgatgacatcgtcaaaatgattgttagtagtaactacaatgaaagcaggtcaactgtttttgggatccaggggatgggaggcatcggtaagacaacattagctcaaaagatatacaatgaacagaggataagggagaaattccaagttcatatatggttgtgcatttctcagaattacacagagaccagtttgttaaaacaggcaatcagaatggctggaggaatttgtgatcagctggagactaagactgagcttctaccgcttctagtggacactattagagggaagagtgtttttcttgtgctggatgatgtttggaaatctgatgtatggattgatcttcttcgtttgcctttcttgagaggtttgaattctcatatccttgtgacctcaagaaacctggatgttttggttgaaatgcatgcaacttatacccacaaggtaaacaaaatgaatgattgcgatggcctagaactgctgatgaagatgtcattagggccatacgagcaaagcagagaattcagtggtgttggatatcaaattgtcaaaaagtgtgatggccttcctcttgccatcaaagttgttgcaggtgttctatctactaaaagaacaagggcagaatgggagagtatccgagatagcaagtggtccattcatggacttcccagagaactaggaggccctctgtacttaagctacagcaacttaccccctgaacttaaacaatgctttctctggtgtgctttgttgccgtcaaattttgtgatacgtcgtgatgctgtcgcttactggtgggttgctgaaggtttcgtgacggaagtgcatgggtactcaatacatgaggttgctgaagagtactaccatgagctaatcagaaggaatcttctgcagccacgaccagagtttgtagacaaaggcgagtcaacaatgcatgatctattgaggtcacttggtcaatttttgacaaaggatcactccatattcatgaacatggaatatagcaaagccttaccaaatctacgccacttatgtatcagcaatgatgtagaagaaatacctgctatagaaaagcaaaagtgcttgaggagcctcctagtttttgataacaaaaacttcatgaaaattaataaggacattttcagagaactcaagcatatccgtgtcttagttctcagtggaacaagcatccaaatcataccagagtcagtgggaaacttcttgttactgaggcttctagatctaagctat</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001048560.1 RefSeq:Os01g0149500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

Os01g0149500

2014-05-25T08:11:41Z

Haidongli: /* Labs working on this gene */

Os01g0149500

2014-05-25T08:02:12Z

Haidongli: /* Evolution */

Os01g0149500

2014-05-25T07:54:11Z

Haidongli: /* Function */

File:1.jpg

2014-05-23T03:15:28Z

Haidongli: uploaded a new version of "File:1.jpg": Gene expression analyses of OsSPY in rice.RNA gel blot analysis ofOsSPYexpression in various organs. Total RNA was isolated from leaf blades (LB), leaf sheaths (LS), stems (ST), flowers (FL), rachises(

Gross morphology of GID1-overexpressor and control plants. Scale
bar, 50 cm.

Os08g0559300

2014-05-23T03:11:45Z

Haidongli: /* Expression */

Please input one-sentence summary here.

==Annotated Information==
===Function===
SPINDLY(SPY) encodes an O-linked N-acetylglucosamine transferase that is considered to be a negative regulator of gibberellin (GA) signaling through an unknown mechanism(Jacobsen and Olszewski,1993; Jacobsenet al., 1996;Swain et al., 2001).Shimada et al(2006) isolated a rice SPINDLY homolog (OsSPY) and produced knockdown transgenic plants in which OsSPY expression was reduced by introducing its antisense or RNAi construct;they find that in knockdown plants, the enhanced elongation of lower internodes was correlated with decreased levels of OsSPY expression, similar to the spindly phenotype of Arabidopsis spy mutants,The suppression of OsSPY function in a GA‐insensitive mutant, gid2, also caused an increase in the phosphorylation of a rice DELLA protein, SLR1, but did not change the amount of SLR1.suggest that OsSPY functions as a negative factor in GA signaling in rice; OsSPY in GA signaling is not via changes in the amount or stability of SLR1, but probably involves control of the suppressive function of SLR1;and OsSPY may play roles both in GA signaling and in the brassinosteroid pathway.

=== Mutation ===
[[File:F1.large.jpg|right|thumb|150px|''spy mutants were more tolerant of high-salinity stress compared with wild-type plants.(Qin al., 2011).'']]

we obtained point mutation mutants of spy-1 and spy-3. Both the spy-1 and spy-3 mutants were generated by EMS mutagenesis screening of Arabidopsis ecotype Columbia (Col) for plants that were more tolerant to PAC during seed germination compared with wild-type plants. The seedlings of both mutants exhibited a GA-overdosed phenotype. The spy-1 mutant exhibited a stronger phenotype than the spy-3 mutant in terms of characteristics affected by GA responses, such as elongated stem, pale leaves, slender seedlings, and male sterility, whereas the spy-3 mutant exhibited only a mild phenotype, and this was probably due to a more severe mutation in the spy-1 mutant.We compared the ability of the spy mutants and wild-type Col plants to survive severe high-salinity stress conditions. If 7-d-old plants were transferred from normal growth conditions to 200 mM NaCl-containing plates after 7 to 10 d, less than 20% of the wild-type plants survived. In contrast, more than 80% of spy-3 and 70% of spy-1 plants continued to grow and retained a greenish color, which suggested that these two mutants were strongly salt tolerant.

[[File:2.large.jpg|right|thumb|150px|''spy-1 and spy-3 mutants were more tolerant of drought stress. (Qin al., 2011).'']]
We also compared plant survival in same tray. In this experiment, the wild-type plants and spy-3 mutants were planted side by side in one soil plot in which the soil water content was more comparable between plants than that in different pots. The earlier bolting and flowering phenotypes of the spy-3 mutant as compared with the wild-type plants were typical GA-elevated phenotypes

===Expression===
The OsSPY gene was expressed in all tested organs, with high levels in inflorescence meristems and low levels in roots( Shimada et al., 2006).
[[File:my computer/D/1.jpg]]

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
1.Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, Aichi 464-8601, Japan

2.Field Production Science Center, University of Tokyo, Nishi-Tokyo, Tokyo 188-0002, Japan

3.RIKEN (The Institute of Physical and Chemical Research), Wako-shi, Saitama 351-0198, Japan

4.Department of Chemistry, Joetsu University of Education, Joetsu-shi, Niigata 943-8512, Japan

5.Science and Technology Research Institute,Chiang Mai University,Chiang Mai 50200,Thailand

6.Molecular Biology Laboratory,Department of Biology, Faculty of Science, Chiang Mai University,Chiang Mai 50200,Thailand

7.Plasma and Beam Physics Research Facility,Department of Physics and Materials Science,Faculty of Science,Chiang Mai University,Chiang Mai 50200

8.Thailand Center of Excellence in Physics,Commission on Higher Education, 328 Si Ayutthaya Road,Bangkok 10400,Thailand

==References==
Jacobsen, S.E. and Olszewski, N.E.(1993) Mutations at theS PINDLY locus of Arabidopsisalter gibberellin signal transduction.Plant Cell, 5, 887–896.

Jacobsen, S.E., Binkowski, K.A. and Olszewski, N.E.(1996) SPINDLY, a tetratricopeptide repeat protein involved in gibberellin signal transduction in Arabidopsis. Proc. Natl Acad. Sci. USA, 93, 9292–9296.

Swain, S.M., Tseng, T.-S. and Olszewski, N.E. (2001) Altered expression ofSPINDLYaffects gibberellin response and plant development.Plant Physiol.126, 1174–1185.

Thornton, T.M., Swain, S.M. and Olszewski, N.E.(1999) Gibberellin signal transduction presents…the SPY who O-GlcNAc’d me.Trends Plant Sci.4, 424–428.

Qin, F., Kodaira, K. S., Maruyama, K., Mizoi, J., Tran, L. S. P., Fujita, Y., ... & Yamaguchi-Shinozaki, K. (2011). SPINDLY, a negative regulator of gibberellic acid signaling, is involved in the plant abiotic stress response. Plant physiology, 157(4), 1900-1913.

==Structured Information==
{{JaponicaGene|
GeneName = Os08g0559300|
Description = Similar to Gibberellin action negative regulator SPY|
Version = NM_001069036.1 GI:115477810 GeneID:4346315|
Length = 7732 bp|
Definition = Oryza sativa Japonica Group Os08g0559300, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 8|Chromosome 8]]|
AP = Chromosome 8:28079564..28087295|
CDS = 28080313..28080681,28081406..28081567,28081662..28081837,28081928..28082003,28082401..28082529 ,28082610..28082729,28082819..28082885,28082979..28083062,28083263..28083357 ,28083674..28083738,28083851..28084049,28084673..28084867,28085113..28085207 ,28085306..28085441,28085512..28085729,28085976..28086103,28086395..28086864 |
GCID = <gbrowseImage1>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcgcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaaatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttgggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtga</cdnaseq>|
AA = <aaseq>MGRPGMDSSEGRESNGVVPERNGGAVPAKQQLDGKDTLRYANIL RSRNKFAEALQLYNNVLEKDEANVEALIGKGICLQAQSLPMQAIECFNEAVRIDPGNA CALTYCGMIYKDEGHLVEAAEAYQKARNADPSYKPAAEFLAIVLTDLGTSLKLAGNTE EGIQKYCEALEVDSHYAPAYYNLGVVYSEMMQFDLALTCYEKAALERPLYAEAYCNMG VIYKNRGELEAAIACYERCLTISPNFEIAKNNMAIALTDLGTKVKIEGDINQGVAYYK KALFYNWHYADAMYNLGVAYGEMLNFEMAIVFYELALHFNPRCAEACNNLGVIYKDRD NLDKAVECYQMALSIKPNFSQSLNNLGVVYTVQGKMDAASSMIQKAIFANSTYAEAYN NLGVLYRDAGSITSAVQAYEKCLQIDPDSRNAGQNRLLALNYIDEGFDDKLYQAHREW GKRFLKLYPQYTSWDNPKVADRPLVIGYVSPDYFTHSVSYFIEAPLAHHDYSNYKVVV YSGVVKADAKTLRFKDKVLKKGGLWRDIYGIDEKKVASLVREDKVDILVELTGHTANN KLGTMACRPAPIQVTWIGYPNTTGLPTIDYRITDSLADPPDTTQKHVEELVRLPESFL CYSPSPEAGPVCPTPAILNGFITFGSFNNLAKITPKVLQVWAKILCAVPNSRLVVKCK PFCCDSIRQKFLSTLAELGLEPLRVDLLPLIHLNHDHMQAYSLMDISLDTFPYAGTTT TCESLYMGVPCVTMAGSVHAHNVGVSLLTKVGLGRLVAKSENEYVSLALDLAADVTAL QELRMSLRGLMAKSPVCDGENFTRGLESAYRNMWRRYCDGDAPALRRLDLLQEEPCSN NNKQDFDDNQVAKLADLKAQRVDAAVDGDKQSQLTAHAAVVGEVQQAPIMVNGVSSPV SSGKVEANGHISR</aaseq>|
DNA = <dnaseqindica>750..1118#1843..2004#2099..2274#2365..2440#2838..2966#3047..3166#3256..3322#3416..3499#3700..3794#4111..4175#4288..4486#5110..5304#5550..5644#5743..5878#5949..6166#6413..6540#6832..7301#aaagaagggaaaaaaaatacgaaaagcaaaaaggaataaacggagggggctaaacaccaaaattccccctctccttctccttcccctcctcccgaaaccctcgccgccgtcgctgtcgaaaccctcgctgctgctgctgccgcacggcgcggcggggtaccgggtccccctccctgcggcggcgcggaggggtaggtctcccccccctcgctgcggcgtggcggtggggggtgcggtgccggctgccgcgccatgatcttgcgccggtggactgcgcctatccaatgtgagctctcctcctacttccgccgcggcattagggttcttgcttcttgagctggaagagttctcttgcgttgcgaacgcctactgtttcctgcgaattcttagtgcttgttcttggattctttccgggggtgttcttaatctgttgtttgcaaaagttctttttttctttttttggctagggttttgaaatttgcaatcttggtgttcttttccctgagtttttactaaaaagctgcggcttttacgcaattcggtggtagtccaaagccgcgtcctttttttctagtctttacgcgcatttggctccaatttttctcctttttttagacgcgttttcttcacgctaatattcgcaactgaaatcttagacctaacgaaccattctaaaattgatgatcgttacattctttctgcagaaaaaaaaaagataaagatagaacaagtaggttactttccgtaccatggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggtatgcgtaagattcttcatgtggattccctgttcttactgttagcttattcgttattgtgaaatctgatagtttttctttttagtttgttgtcataaacactagaagtctcaaatgttgtatatgtggttctctgaaagcaaaatactcaaatgctgaagtaagtgcctcgcacagtcgcactccccaaaataccaggcaatgagacttgggacttaactgccaatttctgacttctgagggtactgcagacctatttttagcaccaagttatatcttgagtggtttgtgtccaagaactgtaactagaaaccaaaacaactagtttgataagttcttgatcaagcatactatgcaagagataaaactgtcgagataatcactcaagaattttccagcagaagattttttattatctccatgacatacttattagcaccgtaaaattttgtgcgtagctggtggatagaatgacatgtgaatgagctaaggctcagctagagctgcaaaaagagtaatacttttgccccttggagaatagtggtccctattaattggcagggttgtaaaaatcctaagttcctaaccaatctaacatataagatcaaaacagttggtcagtccaaaaattctctcggaaaagcttggaatttcgacattgtttctgtatttattatgtgatatatttatccataaatgttctgatggagagaaattgtaacaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcggtaccatcaccagctataatttatatctagatctctattattttgtttttgttatccatttaagttttttaatgtgttcatctgattaatgtagcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtaagctttattttttctgctcatacaaattatgctgattcccaaagtactgttgaaaactaatgttgacttatctttactgtatcccaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtgtggtcttgctaccttttgtttcaaccaaatcaacacattgcttcttgtggtgtcctgcatgtcaatcataatggttgccatcacttgcccatcatattagcagtcatgcgtgacaggcttgaactaagactagtgttgatttgaaaattgtctcaatcatgtgggttaatgaggaaacatcatgataactctatgttttttcgttgcttgtccgctcactttttactctgagcatagcaactctggcaatataattaattttgactaaccttagaatgaatcatatgaaatagacctttttaatttatactttggtatacctttaagaattgttaactctggcatttggtggtgatttttgtatttttctcttatcaattgacatttttcacaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggtcagtcataagaaaaaaatctttaatattctgtacataatgtcctctcttttgctgacactcttttctgttttccaaaggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaagtaagtggaactaattatgcagtatgcaatatctgcagatagtcacaagtatcacaacagattattcattatcttctattctgttgtagatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccaggcatgctcctctgctagtcttgcacaatttctattgggattggatgattgtatcatgttttagcttattgaatatgttgttcttcatatatagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtaatgtttaaccaaccaacatcatgagagcctcttaaattctgccatgtaatttgaaacagcattttccagctgagactcataacaactatataagcactaatcagtaattcatttcattgctttgcataattctctttttgatagctggaatgagactttaatacgtaactgacaatccatcactgctttcaccataggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcaggtactttctttgtcctaagcttgctaattaaaacttgtgtaatctttctgtgttgcatggagaattaaatttatttaagcaggtacagctaataattatacttatccatttagtagcaaactgggaagcatataaatgtttcttccttgtataaatagacaaatacttgtctattgcatgttcataatttgttccaaccttgatgttgcttgcccacggtatctgttttgctgctttttaaaaaaaaatatatatttgtggaatagctatggtttatatttgtaacattcaacttacgatatagcttgttctgcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacaggtcatttagcactttttcacaatatacagtcatatttatgttagattgttttttctgtttttatgtgttagaaattctcaactatatattcactgtattttatattttgcagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggtagatatagccgttccttttcttgtctctattttcctgcattttcacattgtgtcccaaaaaacttgtttagactgtcttgcctcattaagatttaaaatatatatggaaataatttatgcaaaaatattccttttgaaaacagaaatggtagttcaatggtcaataggatagtgaactgtaattatttttcaagataggcctctagactatgtccagtttcaggattaccactttattattgcctagactgaaatgttttaaatagtgggctaagacatttagtggttagccttctcaaacagctatagctatagcggctaaattgtacataagagcaaatatttagctagacccttctcaaacagctatagccggagatttaaaatgcatatcctgaaaccccccaagaaagattatcccagtaacagcgaatatataattggaaatcatccaaggtggctgacaactcggggccatttatttgtgacatatcagttattttccaacttgcatgtttcactacttgtacaaagaaattaggcccaatttatctgcaatgtgttcgagtacctgttttaatcacagttcattcatgttttcttcaattgaaaattatttaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggtacctttgtgcccatgattcataactgtcacgttacctaatttcagaactatttggacagaagtgatatcatcagatcattatgttctgaaaccagcttcttctgatcttgctaacatgattgcttccttttcactctagtcaatgaaactattcaagtgtctaataataaatgattatctgactattctactatgtgattagtgaactgacaggggcttcttttacttgaattgcctgcacaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagtaagacatctgcttttcttgcggtgatagctttctggcatgcacttttatacccacacatctagaacgtgacggtgacaattatgaattatctgcaggcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaaggtgacacataaaacttgagtaatcagttcatttttactggatcactatttattgatttttcttttggcagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataaggtcagttatttagatacttcctccgtttcatattataagactttctagcattgtccacattcatatagatgttgtgtctagatttattaacatctatgtgaatgtggacaatgctagaaagtcttataatatgaaacggagggagtacatcttttatcctatgtttaaacatgttatgtaaacgagtagatccattctactgcattgtatcttaaattgtctgaactttggttggttcctttgcagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttggtacgtgtctatttactctgtggtgtatgtagcaatagatcgaattgctgtacagttttattttcatgctatttgttgatcgacttgttttcttcttttgcatcgccatgtgaagactcggaaaaaaaatcactattgtgctgtctggttttgagttttgatacatataacttgatgattggagcaggaattctagctattgaatctgacctttctttacacagttgaatcaggtcgggtatatgttctccttgccgaattgttggttgacatttactgttattcttgcagggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtgaccaaggggagtcgagcatactccattgatgtgggaaacactgacccactgtagcttgagatgcaccattgctgtggtggacatttgttcttacagaattcgacgctttgcaggcctacactgggtgtctcatttgtctgacacccacaatggataagaaagaagagcacttctgtgccatccgacggtgatttctctggttacactttgacctttgtagcaaatgtaagattgatgggggatatgcttatagagtttgatttgtagaggtataccccctgtgtaggtttatgaacatgatggcttcagttgccatatgttagaagaaacagagaaagaggaggaagcccttgttgtaaatgcagaaaaaacagaactgaaaaactactagaatcaattgacggcggcatttcaacaggctgtgtcatgatg</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001069036.1 RefSeq:Os08g0559300]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 8]]
[[Category:Chromosome 8]]

Os08g0559300

2014-05-23T02:55:23Z

Haidongli: /* References */

Please input one-sentence summary here.

==Annotated Information==
===Function===
SPINDLY(SPY) encodes an O-linked N-acetylglucosamine transferase that is considered to be a negative regulator of gibberellin (GA) signaling through an unknown mechanism(Jacobsen and Olszewski,1993; Jacobsenet al., 1996;Swain et al., 2001).Shimada et al(2006) isolated a rice SPINDLY homolog (OsSPY) and produced knockdown transgenic plants in which OsSPY expression was reduced by introducing its antisense or RNAi construct;they find that in knockdown plants, the enhanced elongation of lower internodes was correlated with decreased levels of OsSPY expression, similar to the spindly phenotype of Arabidopsis spy mutants,The suppression of OsSPY function in a GA‐insensitive mutant, gid2, also caused an increase in the phosphorylation of a rice DELLA protein, SLR1, but did not change the amount of SLR1.suggest that OsSPY functions as a negative factor in GA signaling in rice; OsSPY in GA signaling is not via changes in the amount or stability of SLR1, but probably involves control of the suppressive function of SLR1;and OsSPY may play roles both in GA signaling and in the brassinosteroid pathway.

===Expression===
The OsSPY gene was expressed in all tested organs, with high levels in inflorescence meristems and low levels in roots( Shimada et al., 2006).

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
1.Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, Aichi 464-8601, Japan

2.Field Production Science Center, University of Tokyo, Nishi-Tokyo, Tokyo 188-0002, Japan

3.RIKEN (The Institute of Physical and Chemical Research), Wako-shi, Saitama 351-0198, Japan

4.Department of Chemistry, Joetsu University of Education, Joetsu-shi, Niigata 943-8512, Japan

5.Science and Technology Research Institute,Chiang Mai University,Chiang Mai 50200,Thailand

6.Molecular Biology Laboratory,Department of Biology, Faculty of Science, Chiang Mai University,Chiang Mai 50200,Thailand

7.Plasma and Beam Physics Research Facility,Department of Physics and Materials Science,Faculty of Science,Chiang Mai University,Chiang Mai 50200

8.Thailand Center of Excellence in Physics,Commission on Higher Education, 328 Si Ayutthaya Road,Bangkok 10400,Thailand

==References==
Jacobsen, S.E. and Olszewski, N.E.(1993) Mutations at theS PINDLY locus of Arabidopsisalter gibberellin signal transduction.Plant Cell, 5, 887–896.

Jacobsen, S.E., Binkowski, K.A. and Olszewski, N.E.(1996) SPINDLY, a tetratricopeptide repeat protein involved in gibberellin signal transduction in Arabidopsis. Proc. Natl Acad. Sci. USA, 93, 9292–9296.

Swain, S.M., Tseng, T.-S. and Olszewski, N.E. (2001) Altered expression ofSPINDLYaffects gibberellin response and plant development.Plant Physiol.126, 1174–1185.

Thornton, T.M., Swain, S.M. and Olszewski, N.E.(1999) Gibberellin signal transduction presents…the SPY who O-GlcNAc’d me.Trends Plant Sci.4, 424–428.

==Structured Information==
{{JaponicaGene|
GeneName = Os08g0559300|
Description = Similar to Gibberellin action negative regulator SPY|
Version = NM_001069036.1 GI:115477810 GeneID:4346315|
Length = 7732 bp|
Definition = Oryza sativa Japonica Group Os08g0559300, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 8|Chromosome 8]]|
AP = Chromosome 8:28079564..28087295|
CDS = 28080313..28080681,28081406..28081567,28081662..28081837,28081928..28082003,28082401..28082529 ,28082610..28082729,28082819..28082885,28082979..28083062,28083263..28083357 ,28083674..28083738,28083851..28084049,28084673..28084867,28085113..28085207 ,28085306..28085441,28085512..28085729,28085976..28086103,28086395..28086864 |
GCID = <gbrowseImage1>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcgcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaaatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttgggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtga</cdnaseq>|
AA = <aaseq>MGRPGMDSSEGRESNGVVPERNGGAVPAKQQLDGKDTLRYANIL RSRNKFAEALQLYNNVLEKDEANVEALIGKGICLQAQSLPMQAIECFNEAVRIDPGNA CALTYCGMIYKDEGHLVEAAEAYQKARNADPSYKPAAEFLAIVLTDLGTSLKLAGNTE EGIQKYCEALEVDSHYAPAYYNLGVVYSEMMQFDLALTCYEKAALERPLYAEAYCNMG VIYKNRGELEAAIACYERCLTISPNFEIAKNNMAIALTDLGTKVKIEGDINQGVAYYK KALFYNWHYADAMYNLGVAYGEMLNFEMAIVFYELALHFNPRCAEACNNLGVIYKDRD NLDKAVECYQMALSIKPNFSQSLNNLGVVYTVQGKMDAASSMIQKAIFANSTYAEAYN NLGVLYRDAGSITSAVQAYEKCLQIDPDSRNAGQNRLLALNYIDEGFDDKLYQAHREW GKRFLKLYPQYTSWDNPKVADRPLVIGYVSPDYFTHSVSYFIEAPLAHHDYSNYKVVV YSGVVKADAKTLRFKDKVLKKGGLWRDIYGIDEKKVASLVREDKVDILVELTGHTANN KLGTMACRPAPIQVTWIGYPNTTGLPTIDYRITDSLADPPDTTQKHVEELVRLPESFL CYSPSPEAGPVCPTPAILNGFITFGSFNNLAKITPKVLQVWAKILCAVPNSRLVVKCK PFCCDSIRQKFLSTLAELGLEPLRVDLLPLIHLNHDHMQAYSLMDISLDTFPYAGTTT TCESLYMGVPCVTMAGSVHAHNVGVSLLTKVGLGRLVAKSENEYVSLALDLAADVTAL QELRMSLRGLMAKSPVCDGENFTRGLESAYRNMWRRYCDGDAPALRRLDLLQEEPCSN NNKQDFDDNQVAKLADLKAQRVDAAVDGDKQSQLTAHAAVVGEVQQAPIMVNGVSSPV SSGKVEANGHISR</aaseq>|
DNA = <dnaseqindica>750..1118#1843..2004#2099..2274#2365..2440#2838..2966#3047..3166#3256..3322#3416..3499#3700..3794#4111..4175#4288..4486#5110..5304#5550..5644#5743..5878#5949..6166#6413..6540#6832..7301#aaagaagggaaaaaaaatacgaaaagcaaaaaggaataaacggagggggctaaacaccaaaattccccctctccttctccttcccctcctcccgaaaccctcgccgccgtcgctgtcgaaaccctcgctgctgctgctgccgcacggcgcggcggggtaccgggtccccctccctgcggcggcgcggaggggtaggtctcccccccctcgctgcggcgtggcggtggggggtgcggtgccggctgccgcgccatgatcttgcgccggtggactgcgcctatccaatgtgagctctcctcctacttccgccgcggcattagggttcttgcttcttgagctggaagagttctcttgcgttgcgaacgcctactgtttcctgcgaattcttagtgcttgttcttggattctttccgggggtgttcttaatctgttgtttgcaaaagttctttttttctttttttggctagggttttgaaatttgcaatcttggtgttcttttccctgagtttttactaaaaagctgcggcttttacgcaattcggtggtagtccaaagccgcgtcctttttttctagtctttacgcgcatttggctccaatttttctcctttttttagacgcgttttcttcacgctaatattcgcaactgaaatcttagacctaacgaaccattctaaaattgatgatcgttacattctttctgcagaaaaaaaaaagataaagatagaacaagtaggttactttccgtaccatggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggtatgcgtaagattcttcatgtggattccctgttcttactgttagcttattcgttattgtgaaatctgatagtttttctttttagtttgttgtcataaacactagaagtctcaaatgttgtatatgtggttctctgaaagcaaaatactcaaatgctgaagtaagtgcctcgcacagtcgcactccccaaaataccaggcaatgagacttgggacttaactgccaatttctgacttctgagggtactgcagacctatttttagcaccaagttatatcttgagtggtttgtgtccaagaactgtaactagaaaccaaaacaactagtttgataagttcttgatcaagcatactatgcaagagataaaactgtcgagataatcactcaagaattttccagcagaagattttttattatctccatgacatacttattagcaccgtaaaattttgtgcgtagctggtggatagaatgacatgtgaatgagctaaggctcagctagagctgcaaaaagagtaatacttttgccccttggagaatagtggtccctattaattggcagggttgtaaaaatcctaagttcctaaccaatctaacatataagatcaaaacagttggtcagtccaaaaattctctcggaaaagcttggaatttcgacattgtttctgtatttattatgtgatatatttatccataaatgttctgatggagagaaattgtaacaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcggtaccatcaccagctataatttatatctagatctctattattttgtttttgttatccatttaagttttttaatgtgttcatctgattaatgtagcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtaagctttattttttctgctcatacaaattatgctgattcccaaagtactgttgaaaactaatgttgacttatctttactgtatcccaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtgtggtcttgctaccttttgtttcaaccaaatcaacacattgcttcttgtggtgtcctgcatgtcaatcataatggttgccatcacttgcccatcatattagcagtcatgcgtgacaggcttgaactaagactagtgttgatttgaaaattgtctcaatcatgtgggttaatgaggaaacatcatgataactctatgttttttcgttgcttgtccgctcactttttactctgagcatagcaactctggcaatataattaattttgactaaccttagaatgaatcatatgaaatagacctttttaatttatactttggtatacctttaagaattgttaactctggcatttggtggtgatttttgtatttttctcttatcaattgacatttttcacaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggtcagtcataagaaaaaaatctttaatattctgtacataatgtcctctcttttgctgacactcttttctgttttccaaaggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaagtaagtggaactaattatgcagtatgcaatatctgcagatagtcacaagtatcacaacagattattcattatcttctattctgttgtagatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccaggcatgctcctctgctagtcttgcacaatttctattgggattggatgattgtatcatgttttagcttattgaatatgttgttcttcatatatagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtaatgtttaaccaaccaacatcatgagagcctcttaaattctgccatgtaatttgaaacagcattttccagctgagactcataacaactatataagcactaatcagtaattcatttcattgctttgcataattctctttttgatagctggaatgagactttaatacgtaactgacaatccatcactgctttcaccataggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcaggtactttctttgtcctaagcttgctaattaaaacttgtgtaatctttctgtgttgcatggagaattaaatttatttaagcaggtacagctaataattatacttatccatttagtagcaaactgggaagcatataaatgtttcttccttgtataaatagacaaatacttgtctattgcatgttcataatttgttccaaccttgatgttgcttgcccacggtatctgttttgctgctttttaaaaaaaaatatatatttgtggaatagctatggtttatatttgtaacattcaacttacgatatagcttgttctgcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacaggtcatttagcactttttcacaatatacagtcatatttatgttagattgttttttctgtttttatgtgttagaaattctcaactatatattcactgtattttatattttgcagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggtagatatagccgttccttttcttgtctctattttcctgcattttcacattgtgtcccaaaaaacttgtttagactgtcttgcctcattaagatttaaaatatatatggaaataatttatgcaaaaatattccttttgaaaacagaaatggtagttcaatggtcaataggatagtgaactgtaattatttttcaagataggcctctagactatgtccagtttcaggattaccactttattattgcctagactgaaatgttttaaatagtgggctaagacatttagtggttagccttctcaaacagctatagctatagcggctaaattgtacataagagcaaatatttagctagacccttctcaaacagctatagccggagatttaaaatgcatatcctgaaaccccccaagaaagattatcccagtaacagcgaatatataattggaaatcatccaaggtggctgacaactcggggccatttatttgtgacatatcagttattttccaacttgcatgtttcactacttgtacaaagaaattaggcccaatttatctgcaatgtgttcgagtacctgttttaatcacagttcattcatgttttcttcaattgaaaattatttaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggtacctttgtgcccatgattcataactgtcacgttacctaatttcagaactatttggacagaagtgatatcatcagatcattatgttctgaaaccagcttcttctgatcttgctaacatgattgcttccttttcactctagtcaatgaaactattcaagtgtctaataataaatgattatctgactattctactatgtgattagtgaactgacaggggcttcttttacttgaattgcctgcacaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagtaagacatctgcttttcttgcggtgatagctttctggcatgcacttttatacccacacatctagaacgtgacggtgacaattatgaattatctgcaggcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaaggtgacacataaaacttgagtaatcagttcatttttactggatcactatttattgatttttcttttggcagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataaggtcagttatttagatacttcctccgtttcatattataagactttctagcattgtccacattcatatagatgttgtgtctagatttattaacatctatgtgaatgtggacaatgctagaaagtcttataatatgaaacggagggagtacatcttttatcctatgtttaaacatgttatgtaaacgagtagatccattctactgcattgtatcttaaattgtctgaactttggttggttcctttgcagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttggtacgtgtctatttactctgtggtgtatgtagcaatagatcgaattgctgtacagttttattttcatgctatttgttgatcgacttgttttcttcttttgcatcgccatgtgaagactcggaaaaaaaatcactattgtgctgtctggttttgagttttgatacatataacttgatgattggagcaggaattctagctattgaatctgacctttctttacacagttgaatcaggtcgggtatatgttctccttgccgaattgttggttgacatttactgttattcttgcagggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtgaccaaggggagtcgagcatactccattgatgtgggaaacactgacccactgtagcttgagatgcaccattgctgtggtggacatttgttcttacagaattcgacgctttgcaggcctacactgggtgtctcatttgtctgacacccacaatggataagaaagaagagcacttctgtgccatccgacggtgatttctctggttacactttgacctttgtagcaaatgtaagattgatgggggatatgcttatagagtttgatttgtagaggtataccccctgtgtaggtttatgaacatgatggcttcagttgccatatgttagaagaaacagagaaagaggaggaagcccttgttgtaaatgcagaaaaaacagaactgaaaaactactagaatcaattgacggcggcatttcaacaggctgtgtcatgatg</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001069036.1 RefSeq:Os08g0559300]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 8]]
[[Category:Chromosome 8]]

Os08g0559300

2014-05-23T02:52:51Z

Haidongli: /* Expression */

Please input one-sentence summary here.

==Annotated Information==
===Function===
SPINDLY(SPY) encodes an O-linked N-acetylglucosamine transferase that is considered to be a negative regulator of gibberellin (GA) signaling through an unknown mechanism(Jacobsen and Olszewski,1993; Jacobsenet al., 1996;Swain et al., 2001).Shimada et al(2006) isolated a rice SPINDLY homolog (OsSPY) and produced knockdown transgenic plants in which OsSPY expression was reduced by introducing its antisense or RNAi construct;they find that in knockdown plants, the enhanced elongation of lower internodes was correlated with decreased levels of OsSPY expression, similar to the spindly phenotype of Arabidopsis spy mutants,The suppression of OsSPY function in a GA‐insensitive mutant, gid2, also caused an increase in the phosphorylation of a rice DELLA protein, SLR1, but did not change the amount of SLR1.suggest that OsSPY functions as a negative factor in GA signaling in rice; OsSPY in GA signaling is not via changes in the amount or stability of SLR1, but probably involves control of the suppressive function of SLR1;and OsSPY may play roles both in GA signaling and in the brassinosteroid pathway.

===Expression===
The OsSPY gene was expressed in all tested organs, with high levels in inflorescence meristems and low levels in roots( Shimada et al., 2006).

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
1.Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, Aichi 464-8601, Japan

2.Field Production Science Center, University of Tokyo, Nishi-Tokyo, Tokyo 188-0002, Japan

3.RIKEN (The Institute of Physical and Chemical Research), Wako-shi, Saitama 351-0198, Japan

4.Department of Chemistry, Joetsu University of Education, Joetsu-shi, Niigata 943-8512, Japan

5.Science and Technology Research Institute,Chiang Mai University,Chiang Mai 50200,Thailand

6.Molecular Biology Laboratory,Department of Biology, Faculty of Science, Chiang Mai University,Chiang Mai 50200,Thailand

7.Plasma and Beam Physics Research Facility,Department of Physics and Materials Science,Faculty of Science,Chiang Mai University,Chiang Mai 50200

8.Thailand Center of Excellence in Physics,Commission on Higher Education, 328 Si Ayutthaya Road,Bangkok 10400,Thailand

==References==
Jacobsen, S.E. and Olszewski, N.E.(1993) Mutations at theS PINDLY locus of Arabidopsisalter gibberellin signal transduction.Plant Cell, 5, 887–896.

Jacobsen, S.E., Binkowski, K.A. and Olszewski, N.E.(1996) SPINDLY, a tetratricopeptide repeat protein involved in gibberellin signal transduction in Arabidopsis. Proc. Natl Acad. Sci. USA, 93, 9292–9296.

Swain, S.M., Tseng, T.-S. and Olszewski, N.E. (2001) Altered expression ofSPINDLYaffects gibberellin response and plant development.Plant Physiol.126, 1174–1185.

Thornton, T.M., Swain, S.M. and Olszewski, N.E.(1999) Gibberellin signal transduction presents…the SPY who O-GlcNAc’d me.Trends Plant Sci.4, 424–428.
<references>
<ref name="ref5">Qin, F., Kodaira, K. S., Maruyama, K., Mizoi, J., Tran, L. S. P., Fujita, Y., ... & Yamaguchi-Shinozaki, K. (2011). SPINDLY, a negative regulator of gibberellic acid signaling, is involved in the plant abiotic stress response. Plant physiology, 157(4), 1900-1913.</ref>

<ref name="ref6">Swain, S. M., Tseng, T. S., Thornton, T. M., Gopalraj, M., & Olszewski, N. E. (2002). SPINDLY is a nuclear-localized repressor of gibberellin signal transduction expressed throughout the plant. Plant physiology, 129(2), 605-615.</ref>
</references>
==Structured Information==
{{JaponicaGene|
GeneName = Os08g0559300|
Description = Similar to Gibberellin action negative regulator SPY|
Version = NM_001069036.1 GI:115477810 GeneID:4346315|
Length = 7732 bp|
Definition = Oryza sativa Japonica Group Os08g0559300, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 8|Chromosome 8]]|
AP = Chromosome 8:28079564..28087295|
CDS = 28080313..28080681,28081406..28081567,28081662..28081837,28081928..28082003,28082401..28082529 ,28082610..28082729,28082819..28082885,28082979..28083062,28083263..28083357 ,28083674..28083738,28083851..28084049,28084673..28084867,28085113..28085207 ,28085306..28085441,28085512..28085729,28085976..28086103,28086395..28086864 |
GCID = <gbrowseImage1>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcgcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaaatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttgggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtga</cdnaseq>|
AA = <aaseq>MGRPGMDSSEGRESNGVVPERNGGAVPAKQQLDGKDTLRYANIL RSRNKFAEALQLYNNVLEKDEANVEALIGKGICLQAQSLPMQAIECFNEAVRIDPGNA CALTYCGMIYKDEGHLVEAAEAYQKARNADPSYKPAAEFLAIVLTDLGTSLKLAGNTE EGIQKYCEALEVDSHYAPAYYNLGVVYSEMMQFDLALTCYEKAALERPLYAEAYCNMG VIYKNRGELEAAIACYERCLTISPNFEIAKNNMAIALTDLGTKVKIEGDINQGVAYYK KALFYNWHYADAMYNLGVAYGEMLNFEMAIVFYELALHFNPRCAEACNNLGVIYKDRD NLDKAVECYQMALSIKPNFSQSLNNLGVVYTVQGKMDAASSMIQKAIFANSTYAEAYN NLGVLYRDAGSITSAVQAYEKCLQIDPDSRNAGQNRLLALNYIDEGFDDKLYQAHREW GKRFLKLYPQYTSWDNPKVADRPLVIGYVSPDYFTHSVSYFIEAPLAHHDYSNYKVVV YSGVVKADAKTLRFKDKVLKKGGLWRDIYGIDEKKVASLVREDKVDILVELTGHTANN KLGTMACRPAPIQVTWIGYPNTTGLPTIDYRITDSLADPPDTTQKHVEELVRLPESFL CYSPSPEAGPVCPTPAILNGFITFGSFNNLAKITPKVLQVWAKILCAVPNSRLVVKCK PFCCDSIRQKFLSTLAELGLEPLRVDLLPLIHLNHDHMQAYSLMDISLDTFPYAGTTT TCESLYMGVPCVTMAGSVHAHNVGVSLLTKVGLGRLVAKSENEYVSLALDLAADVTAL QELRMSLRGLMAKSPVCDGENFTRGLESAYRNMWRRYCDGDAPALRRLDLLQEEPCSN NNKQDFDDNQVAKLADLKAQRVDAAVDGDKQSQLTAHAAVVGEVQQAPIMVNGVSSPV SSGKVEANGHISR</aaseq>|
DNA = <dnaseqindica>750..1118#1843..2004#2099..2274#2365..2440#2838..2966#3047..3166#3256..3322#3416..3499#3700..3794#4111..4175#4288..4486#5110..5304#5550..5644#5743..5878#5949..6166#6413..6540#6832..7301#aaagaagggaaaaaaaatacgaaaagcaaaaaggaataaacggagggggctaaacaccaaaattccccctctccttctccttcccctcctcccgaaaccctcgccgccgtcgctgtcgaaaccctcgctgctgctgctgccgcacggcgcggcggggtaccgggtccccctccctgcggcggcgcggaggggtaggtctcccccccctcgctgcggcgtggcggtggggggtgcggtgccggctgccgcgccatgatcttgcgccggtggactgcgcctatccaatgtgagctctcctcctacttccgccgcggcattagggttcttgcttcttgagctggaagagttctcttgcgttgcgaacgcctactgtttcctgcgaattcttagtgcttgttcttggattctttccgggggtgttcttaatctgttgtttgcaaaagttctttttttctttttttggctagggttttgaaatttgcaatcttggtgttcttttccctgagtttttactaaaaagctgcggcttttacgcaattcggtggtagtccaaagccgcgtcctttttttctagtctttacgcgcatttggctccaatttttctcctttttttagacgcgttttcttcacgctaatattcgcaactgaaatcttagacctaacgaaccattctaaaattgatgatcgttacattctttctgcagaaaaaaaaaagataaagatagaacaagtaggttactttccgtaccatggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggtatgcgtaagattcttcatgtggattccctgttcttactgttagcttattcgttattgtgaaatctgatagtttttctttttagtttgttgtcataaacactagaagtctcaaatgttgtatatgtggttctctgaaagcaaaatactcaaatgctgaagtaagtgcctcgcacagtcgcactccccaaaataccaggcaatgagacttgggacttaactgccaatttctgacttctgagggtactgcagacctatttttagcaccaagttatatcttgagtggtttgtgtccaagaactgtaactagaaaccaaaacaactagtttgataagttcttgatcaagcatactatgcaagagataaaactgtcgagataatcactcaagaattttccagcagaagattttttattatctccatgacatacttattagcaccgtaaaattttgtgcgtagctggtggatagaatgacatgtgaatgagctaaggctcagctagagctgcaaaaagagtaatacttttgccccttggagaatagtggtccctattaattggcagggttgtaaaaatcctaagttcctaaccaatctaacatataagatcaaaacagttggtcagtccaaaaattctctcggaaaagcttggaatttcgacattgtttctgtatttattatgtgatatatttatccataaatgttctgatggagagaaattgtaacaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcggtaccatcaccagctataatttatatctagatctctattattttgtttttgttatccatttaagttttttaatgtgttcatctgattaatgtagcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtaagctttattttttctgctcatacaaattatgctgattcccaaagtactgttgaaaactaatgttgacttatctttactgtatcccaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtgtggtcttgctaccttttgtttcaaccaaatcaacacattgcttcttgtggtgtcctgcatgtcaatcataatggttgccatcacttgcccatcatattagcagtcatgcgtgacaggcttgaactaagactagtgttgatttgaaaattgtctcaatcatgtgggttaatgaggaaacatcatgataactctatgttttttcgttgcttgtccgctcactttttactctgagcatagcaactctggcaatataattaattttgactaaccttagaatgaatcatatgaaatagacctttttaatttatactttggtatacctttaagaattgttaactctggcatttggtggtgatttttgtatttttctcttatcaattgacatttttcacaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggtcagtcataagaaaaaaatctttaatattctgtacataatgtcctctcttttgctgacactcttttctgttttccaaaggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaagtaagtggaactaattatgcagtatgcaatatctgcagatagtcacaagtatcacaacagattattcattatcttctattctgttgtagatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccaggcatgctcctctgctagtcttgcacaatttctattgggattggatgattgtatcatgttttagcttattgaatatgttgttcttcatatatagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtaatgtttaaccaaccaacatcatgagagcctcttaaattctgccatgtaatttgaaacagcattttccagctgagactcataacaactatataagcactaatcagtaattcatttcattgctttgcataattctctttttgatagctggaatgagactttaatacgtaactgacaatccatcactgctttcaccataggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcaggtactttctttgtcctaagcttgctaattaaaacttgtgtaatctttctgtgttgcatggagaattaaatttatttaagcaggtacagctaataattatacttatccatttagtagcaaactgggaagcatataaatgtttcttccttgtataaatagacaaatacttgtctattgcatgttcataatttgttccaaccttgatgttgcttgcccacggtatctgttttgctgctttttaaaaaaaaatatatatttgtggaatagctatggtttatatttgtaacattcaacttacgatatagcttgttctgcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacaggtcatttagcactttttcacaatatacagtcatatttatgttagattgttttttctgtttttatgtgttagaaattctcaactatatattcactgtattttatattttgcagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggtagatatagccgttccttttcttgtctctattttcctgcattttcacattgtgtcccaaaaaacttgtttagactgtcttgcctcattaagatttaaaatatatatggaaataatttatgcaaaaatattccttttgaaaacagaaatggtagttcaatggtcaataggatagtgaactgtaattatttttcaagataggcctctagactatgtccagtttcaggattaccactttattattgcctagactgaaatgttttaaatagtgggctaagacatttagtggttagccttctcaaacagctatagctatagcggctaaattgtacataagagcaaatatttagctagacccttctcaaacagctatagccggagatttaaaatgcatatcctgaaaccccccaagaaagattatcccagtaacagcgaatatataattggaaatcatccaaggtggctgacaactcggggccatttatttgtgacatatcagttattttccaacttgcatgtttcactacttgtacaaagaaattaggcccaatttatctgcaatgtgttcgagtacctgttttaatcacagttcattcatgttttcttcaattgaaaattatttaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggtacctttgtgcccatgattcataactgtcacgttacctaatttcagaactatttggacagaagtgatatcatcagatcattatgttctgaaaccagcttcttctgatcttgctaacatgattgcttccttttcactctagtcaatgaaactattcaagtgtctaataataaatgattatctgactattctactatgtgattagtgaactgacaggggcttcttttacttgaattgcctgcacaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagtaagacatctgcttttcttgcggtgatagctttctggcatgcacttttatacccacacatctagaacgtgacggtgacaattatgaattatctgcaggcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaaggtgacacataaaacttgagtaatcagttcatttttactggatcactatttattgatttttcttttggcagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataaggtcagttatttagatacttcctccgtttcatattataagactttctagcattgtccacattcatatagatgttgtgtctagatttattaacatctatgtgaatgtggacaatgctagaaagtcttataatatgaaacggagggagtacatcttttatcctatgtttaaacatgttatgtaaacgagtagatccattctactgcattgtatcttaaattgtctgaactttggttggttcctttgcagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttggtacgtgtctatttactctgtggtgtatgtagcaatagatcgaattgctgtacagttttattttcatgctatttgttgatcgacttgttttcttcttttgcatcgccatgtgaagactcggaaaaaaaatcactattgtgctgtctggttttgagttttgatacatataacttgatgattggagcaggaattctagctattgaatctgacctttctttacacagttgaatcaggtcgggtatatgttctccttgccgaattgttggttgacatttactgttattcttgcagggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtgaccaaggggagtcgagcatactccattgatgtgggaaacactgacccactgtagcttgagatgcaccattgctgtggtggacatttgttcttacagaattcgacgctttgcaggcctacactgggtgtctcatttgtctgacacccacaatggataagaaagaagagcacttctgtgccatccgacggtgatttctctggttacactttgacctttgtagcaaatgtaagattgatgggggatatgcttatagagtttgatttgtagaggtataccccctgtgtaggtttatgaacatgatggcttcagttgccatatgttagaagaaacagagaaagaggaggaagcccttgttgtaaatgcagaaaaaacagaactgaaaaactactagaatcaattgacggcggcatttcaacaggctgtgtcatgatg</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001069036.1 RefSeq:Os08g0559300]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 8]]
[[Category:Chromosome 8]]

Os08g0559300

2014-05-23T01:46:07Z

Haidongli: /* References */

Please input one-sentence summary here.

==Annotated Information==
===Function===
SPINDLY(SPY) encodes an O-linked N-acetylglucosamine transferase that is considered to be a negative regulator of gibberellin (GA) signaling through an unknown mechanism(Jacobsen and Olszewski,1993; Jacobsenet al., 1996;Swain et al., 2001).Shimada et al(2006) isolated a rice SPINDLY homolog (OsSPY) and produced knockdown transgenic plants in which OsSPY expression was reduced by introducing its antisense or RNAi construct;they find that in knockdown plants, the enhanced elongation of lower internodes was correlated with decreased levels of OsSPY expression, similar to the spindly phenotype of Arabidopsis spy mutants,The suppression of OsSPY function in a GA‐insensitive mutant, gid2, also caused an increase in the phosphorylation of a rice DELLA protein, SLR1, but did not change the amount of SLR1.suggest that OsSPY functions as a negative factor in GA signaling in rice; OsSPY in GA signaling is not via changes in the amount or stability of SLR1, but probably involves control of the suppressive function of SLR1;and OsSPY may play roles both in GA signaling and in the brassinosteroid pathway.

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
1.Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, Aichi 464-8601, Japan

2.Field Production Science Center, University of Tokyo, Nishi-Tokyo, Tokyo 188-0002, Japan

3.RIKEN (The Institute of Physical and Chemical Research), Wako-shi, Saitama 351-0198, Japan

4.Department of Chemistry, Joetsu University of Education, Joetsu-shi, Niigata 943-8512, Japan

5.Science and Technology Research Institute,Chiang Mai University,Chiang Mai 50200,Thailand

6.Molecular Biology Laboratory,Department of Biology, Faculty of Science, Chiang Mai University,Chiang Mai 50200,Thailand

7.Plasma and Beam Physics Research Facility,Department of Physics and Materials Science,Faculty of Science,Chiang Mai University,Chiang Mai 50200

8.Thailand Center of Excellence in Physics,Commission on Higher Education, 328 Si Ayutthaya Road,Bangkok 10400,Thailand

==References==
Jacobsen, S.E. and Olszewski, N.E.(1993) Mutations at theS PINDLY locus of Arabidopsisalter gibberellin signal transduction.Plant Cell, 5, 887–896.

Jacobsen, S.E., Binkowski, K.A. and Olszewski, N.E.(1996) SPINDLY, a tetratricopeptide repeat protein involved in gibberellin signal transduction in Arabidopsis. Proc. Natl Acad. Sci. USA, 93, 9292–9296.

Swain, S.M., Tseng, T.-S. and Olszewski, N.E. (2001) Altered expression ofSPINDLYaffects gibberellin response and plant development.Plant Physiol.126, 1174–1185.

Thornton, T.M., Swain, S.M. and Olszewski, N.E.(1999) Gibberellin signal transduction presents…the SPY who O-GlcNAc’d me.Trends Plant Sci.4, 424–428.

==Structured Information==
{{JaponicaGene|
GeneName = Os08g0559300|
Description = Similar to Gibberellin action negative regulator SPY|
Version = NM_001069036.1 GI:115477810 GeneID:4346315|
Length = 7732 bp|
Definition = Oryza sativa Japonica Group Os08g0559300, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 8|Chromosome 8]]|
AP = Chromosome 8:28079564..28087295|
CDS = 28080313..28080681,28081406..28081567,28081662..28081837,28081928..28082003,28082401..28082529 ,28082610..28082729,28082819..28082885,28082979..28083062,28083263..28083357 ,28083674..28083738,28083851..28084049,28084673..28084867,28085113..28085207 ,28085306..28085441,28085512..28085729,28085976..28086103,28086395..28086864 |
GCID = <gbrowseImage1>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcgcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaaatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttgggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtga</cdnaseq>|
AA = <aaseq>MGRPGMDSSEGRESNGVVPERNGGAVPAKQQLDGKDTLRYANIL RSRNKFAEALQLYNNVLEKDEANVEALIGKGICLQAQSLPMQAIECFNEAVRIDPGNA CALTYCGMIYKDEGHLVEAAEAYQKARNADPSYKPAAEFLAIVLTDLGTSLKLAGNTE EGIQKYCEALEVDSHYAPAYYNLGVVYSEMMQFDLALTCYEKAALERPLYAEAYCNMG VIYKNRGELEAAIACYERCLTISPNFEIAKNNMAIALTDLGTKVKIEGDINQGVAYYK KALFYNWHYADAMYNLGVAYGEMLNFEMAIVFYELALHFNPRCAEACNNLGVIYKDRD NLDKAVECYQMALSIKPNFSQSLNNLGVVYTVQGKMDAASSMIQKAIFANSTYAEAYN NLGVLYRDAGSITSAVQAYEKCLQIDPDSRNAGQNRLLALNYIDEGFDDKLYQAHREW GKRFLKLYPQYTSWDNPKVADRPLVIGYVSPDYFTHSVSYFIEAPLAHHDYSNYKVVV YSGVVKADAKTLRFKDKVLKKGGLWRDIYGIDEKKVASLVREDKVDILVELTGHTANN KLGTMACRPAPIQVTWIGYPNTTGLPTIDYRITDSLADPPDTTQKHVEELVRLPESFL CYSPSPEAGPVCPTPAILNGFITFGSFNNLAKITPKVLQVWAKILCAVPNSRLVVKCK PFCCDSIRQKFLSTLAELGLEPLRVDLLPLIHLNHDHMQAYSLMDISLDTFPYAGTTT TCESLYMGVPCVTMAGSVHAHNVGVSLLTKVGLGRLVAKSENEYVSLALDLAADVTAL QELRMSLRGLMAKSPVCDGENFTRGLESAYRNMWRRYCDGDAPALRRLDLLQEEPCSN NNKQDFDDNQVAKLADLKAQRVDAAVDGDKQSQLTAHAAVVGEVQQAPIMVNGVSSPV SSGKVEANGHISR</aaseq>|
DNA = <dnaseqindica>750..1118#1843..2004#2099..2274#2365..2440#2838..2966#3047..3166#3256..3322#3416..3499#3700..3794#4111..4175#4288..4486#5110..5304#5550..5644#5743..5878#5949..6166#6413..6540#6832..7301#aaagaagggaaaaaaaatacgaaaagcaaaaaggaataaacggagggggctaaacaccaaaattccccctctccttctccttcccctcctcccgaaaccctcgccgccgtcgctgtcgaaaccctcgctgctgctgctgccgcacggcgcggcggggtaccgggtccccctccctgcggcggcgcggaggggtaggtctcccccccctcgctgcggcgtggcggtggggggtgcggtgccggctgccgcgccatgatcttgcgccggtggactgcgcctatccaatgtgagctctcctcctacttccgccgcggcattagggttcttgcttcttgagctggaagagttctcttgcgttgcgaacgcctactgtttcctgcgaattcttagtgcttgttcttggattctttccgggggtgttcttaatctgttgtttgcaaaagttctttttttctttttttggctagggttttgaaatttgcaatcttggtgttcttttccctgagtttttactaaaaagctgcggcttttacgcaattcggtggtagtccaaagccgcgtcctttttttctagtctttacgcgcatttggctccaatttttctcctttttttagacgcgttttcttcacgctaatattcgcaactgaaatcttagacctaacgaaccattctaaaattgatgatcgttacattctttctgcagaaaaaaaaaagataaagatagaacaagtaggttactttccgtaccatggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggtatgcgtaagattcttcatgtggattccctgttcttactgttagcttattcgttattgtgaaatctgatagtttttctttttagtttgttgtcataaacactagaagtctcaaatgttgtatatgtggttctctgaaagcaaaatactcaaatgctgaagtaagtgcctcgcacagtcgcactccccaaaataccaggcaatgagacttgggacttaactgccaatttctgacttctgagggtactgcagacctatttttagcaccaagttatatcttgagtggtttgtgtccaagaactgtaactagaaaccaaaacaactagtttgataagttcttgatcaagcatactatgcaagagataaaactgtcgagataatcactcaagaattttccagcagaagattttttattatctccatgacatacttattagcaccgtaaaattttgtgcgtagctggtggatagaatgacatgtgaatgagctaaggctcagctagagctgcaaaaagagtaatacttttgccccttggagaatagtggtccctattaattggcagggttgtaaaaatcctaagttcctaaccaatctaacatataagatcaaaacagttggtcagtccaaaaattctctcggaaaagcttggaatttcgacattgtttctgtatttattatgtgatatatttatccataaatgttctgatggagagaaattgtaacaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcggtaccatcaccagctataatttatatctagatctctattattttgtttttgttatccatttaagttttttaatgtgttcatctgattaatgtagcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtaagctttattttttctgctcatacaaattatgctgattcccaaagtactgttgaaaactaatgttgacttatctttactgtatcccaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtgtggtcttgctaccttttgtttcaaccaaatcaacacattgcttcttgtggtgtcctgcatgtcaatcataatggttgccatcacttgcccatcatattagcagtcatgcgtgacaggcttgaactaagactagtgttgatttgaaaattgtctcaatcatgtgggttaatgaggaaacatcatgataactctatgttttttcgttgcttgtccgctcactttttactctgagcatagcaactctggcaatataattaattttgactaaccttagaatgaatcatatgaaatagacctttttaatttatactttggtatacctttaagaattgttaactctggcatttggtggtgatttttgtatttttctcttatcaattgacatttttcacaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggtcagtcataagaaaaaaatctttaatattctgtacataatgtcctctcttttgctgacactcttttctgttttccaaaggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaagtaagtggaactaattatgcagtatgcaatatctgcagatagtcacaagtatcacaacagattattcattatcttctattctgttgtagatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccaggcatgctcctctgctagtcttgcacaatttctattgggattggatgattgtatcatgttttagcttattgaatatgttgttcttcatatatagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtaatgtttaaccaaccaacatcatgagagcctcttaaattctgccatgtaatttgaaacagcattttccagctgagactcataacaactatataagcactaatcagtaattcatttcattgctttgcataattctctttttgatagctggaatgagactttaatacgtaactgacaatccatcactgctttcaccataggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcaggtactttctttgtcctaagcttgctaattaaaacttgtgtaatctttctgtgttgcatggagaattaaatttatttaagcaggtacagctaataattatacttatccatttagtagcaaactgggaagcatataaatgtttcttccttgtataaatagacaaatacttgtctattgcatgttcataatttgttccaaccttgatgttgcttgcccacggtatctgttttgctgctttttaaaaaaaaatatatatttgtggaatagctatggtttatatttgtaacattcaacttacgatatagcttgttctgcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacaggtcatttagcactttttcacaatatacagtcatatttatgttagattgttttttctgtttttatgtgttagaaattctcaactatatattcactgtattttatattttgcagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggtagatatagccgttccttttcttgtctctattttcctgcattttcacattgtgtcccaaaaaacttgtttagactgtcttgcctcattaagatttaaaatatatatggaaataatttatgcaaaaatattccttttgaaaacagaaatggtagttcaatggtcaataggatagtgaactgtaattatttttcaagataggcctctagactatgtccagtttcaggattaccactttattattgcctagactgaaatgttttaaatagtgggctaagacatttagtggttagccttctcaaacagctatagctatagcggctaaattgtacataagagcaaatatttagctagacccttctcaaacagctatagccggagatttaaaatgcatatcctgaaaccccccaagaaagattatcccagtaacagcgaatatataattggaaatcatccaaggtggctgacaactcggggccatttatttgtgacatatcagttattttccaacttgcatgtttcactacttgtacaaagaaattaggcccaatttatctgcaatgtgttcgagtacctgttttaatcacagttcattcatgttttcttcaattgaaaattatttaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggtacctttgtgcccatgattcataactgtcacgttacctaatttcagaactatttggacagaagtgatatcatcagatcattatgttctgaaaccagcttcttctgatcttgctaacatgattgcttccttttcactctagtcaatgaaactattcaagtgtctaataataaatgattatctgactattctactatgtgattagtgaactgacaggggcttcttttacttgaattgcctgcacaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagtaagacatctgcttttcttgcggtgatagctttctggcatgcacttttatacccacacatctagaacgtgacggtgacaattatgaattatctgcaggcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaaggtgacacataaaacttgagtaatcagttcatttttactggatcactatttattgatttttcttttggcagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataaggtcagttatttagatacttcctccgtttcatattataagactttctagcattgtccacattcatatagatgttgtgtctagatttattaacatctatgtgaatgtggacaatgctagaaagtcttataatatgaaacggagggagtacatcttttatcctatgtttaaacatgttatgtaaacgagtagatccattctactgcattgtatcttaaattgtctgaactttggttggttcctttgcagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttggtacgtgtctatttactctgtggtgtatgtagcaatagatcgaattgctgtacagttttattttcatgctatttgttgatcgacttgttttcttcttttgcatcgccatgtgaagactcggaaaaaaaatcactattgtgctgtctggttttgagttttgatacatataacttgatgattggagcaggaattctagctattgaatctgacctttctttacacagttgaatcaggtcgggtatatgttctccttgccgaattgttggttgacatttactgttattcttgcagggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtgaccaaggggagtcgagcatactccattgatgtgggaaacactgacccactgtagcttgagatgcaccattgctgtggtggacatttgttcttacagaattcgacgctttgcaggcctacactgggtgtctcatttgtctgacacccacaatggataagaaagaagagcacttctgtgccatccgacggtgatttctctggttacactttgacctttgtagcaaatgtaagattgatgggggatatgcttatagagtttgatttgtagaggtataccccctgtgtaggtttatgaacatgatggcttcagttgccatatgttagaagaaacagagaaagaggaggaagcccttgttgtaaatgcagaaaaaacagaactgaaaaactactagaatcaattgacggcggcatttcaacaggctgtgtcatgatg</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001069036.1 RefSeq:Os08g0559300]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 8]]
[[Category:Chromosome 8]]

Os08g0559300

2014-05-23T01:43:57Z

Haidongli: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==
===Function===
SPINDLY(SPY) encodes an O-linked N-acetylglucosamine transferase that is considered to be a negative regulator of gibberellin (GA) signaling through an unknown mechanism(Jacobsen and Olszewski,1993; Jacobsenet al., 1996;Swain et al., 2001).Shimada et al(2006) isolated a rice SPINDLY homolog (OsSPY) and produced knockdown transgenic plants in which OsSPY expression was reduced by introducing its antisense or RNAi construct;they find that in knockdown plants, the enhanced elongation of lower internodes was correlated with decreased levels of OsSPY expression, similar to the spindly phenotype of Arabidopsis spy mutants,The suppression of OsSPY function in a GA‐insensitive mutant, gid2, also caused an increase in the phosphorylation of a rice DELLA protein, SLR1, but did not change the amount of SLR1.suggest that OsSPY functions as a negative factor in GA signaling in rice; OsSPY in GA signaling is not via changes in the amount or stability of SLR1, but probably involves control of the suppressive function of SLR1;and OsSPY may play roles both in GA signaling and in the brassinosteroid pathway.

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
1.Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, Aichi 464-8601, Japan

2.Field Production Science Center, University of Tokyo, Nishi-Tokyo, Tokyo 188-0002, Japan

3.RIKEN (The Institute of Physical and Chemical Research), Wako-shi, Saitama 351-0198, Japan

4.Department of Chemistry, Joetsu University of Education, Joetsu-shi, Niigata 943-8512, Japan

5.Science and Technology Research Institute,Chiang Mai University,Chiang Mai 50200,Thailand

6.Molecular Biology Laboratory,Department of Biology, Faculty of Science, Chiang Mai University,Chiang Mai 50200,Thailand

7.Plasma and Beam Physics Research Facility,Department of Physics and Materials Science,Faculty of Science,Chiang Mai University,Chiang Mai 50200

8.Thailand Center of Excellence in Physics,Commission on Higher Education, 328 Si Ayutthaya Road,Bangkok 10400,Thailand

==References==
Jacobsen, S.E. and Olszewski, N.E.(1993) Mutations at theS PINDLY locus of Arabidopsisalter gibberellin signal transduction.Plant Cell, 5, 887–896.
Jacobsen, S.E., Binkowski, K.A. and Olszewski, N.E.(1996) SPINDLY, a tetratricopeptide repeat protein involved in gibberellin signal transduction in Arabidopsis. Proc. Natl Acad. Sci. USA, 93, 9292–9296.
Swain, S.M., Tseng, T.-S. and Olszewski, N.E. (2001) Altered expression ofSPINDLYaffects gibberellin response and plant development.Plant Physiol.126, 1174–1185.
Thornton, T.M., Swain, S.M. and Olszewski, N.E.(1999) Gibberellin signal transduction presents…the SPY who O-GlcNAc’d me.Trends Plant Sci.4, 424–428.

==Structured Information==
{{JaponicaGene|
GeneName = Os08g0559300|
Description = Similar to Gibberellin action negative regulator SPY|
Version = NM_001069036.1 GI:115477810 GeneID:4346315|
Length = 7732 bp|
Definition = Oryza sativa Japonica Group Os08g0559300, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 8|Chromosome 8]]|
AP = Chromosome 8:28079564..28087295|
CDS = 28080313..28080681,28081406..28081567,28081662..28081837,28081928..28082003,28082401..28082529 ,28082610..28082729,28082819..28082885,28082979..28083062,28083263..28083357 ,28083674..28083738,28083851..28084049,28084673..28084867,28085113..28085207 ,28085306..28085441,28085512..28085729,28085976..28086103,28086395..28086864 |
GCID = <gbrowseImage1>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcgcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaaatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttgggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtga</cdnaseq>|
AA = <aaseq>MGRPGMDSSEGRESNGVVPERNGGAVPAKQQLDGKDTLRYANIL RSRNKFAEALQLYNNVLEKDEANVEALIGKGICLQAQSLPMQAIECFNEAVRIDPGNA CALTYCGMIYKDEGHLVEAAEAYQKARNADPSYKPAAEFLAIVLTDLGTSLKLAGNTE EGIQKYCEALEVDSHYAPAYYNLGVVYSEMMQFDLALTCYEKAALERPLYAEAYCNMG VIYKNRGELEAAIACYERCLTISPNFEIAKNNMAIALTDLGTKVKIEGDINQGVAYYK KALFYNWHYADAMYNLGVAYGEMLNFEMAIVFYELALHFNPRCAEACNNLGVIYKDRD NLDKAVECYQMALSIKPNFSQSLNNLGVVYTVQGKMDAASSMIQKAIFANSTYAEAYN NLGVLYRDAGSITSAVQAYEKCLQIDPDSRNAGQNRLLALNYIDEGFDDKLYQAHREW GKRFLKLYPQYTSWDNPKVADRPLVIGYVSPDYFTHSVSYFIEAPLAHHDYSNYKVVV YSGVVKADAKTLRFKDKVLKKGGLWRDIYGIDEKKVASLVREDKVDILVELTGHTANN KLGTMACRPAPIQVTWIGYPNTTGLPTIDYRITDSLADPPDTTQKHVEELVRLPESFL CYSPSPEAGPVCPTPAILNGFITFGSFNNLAKITPKVLQVWAKILCAVPNSRLVVKCK PFCCDSIRQKFLSTLAELGLEPLRVDLLPLIHLNHDHMQAYSLMDISLDTFPYAGTTT TCESLYMGVPCVTMAGSVHAHNVGVSLLTKVGLGRLVAKSENEYVSLALDLAADVTAL QELRMSLRGLMAKSPVCDGENFTRGLESAYRNMWRRYCDGDAPALRRLDLLQEEPCSN NNKQDFDDNQVAKLADLKAQRVDAAVDGDKQSQLTAHAAVVGEVQQAPIMVNGVSSPV SSGKVEANGHISR</aaseq>|
DNA = <dnaseqindica>750..1118#1843..2004#2099..2274#2365..2440#2838..2966#3047..3166#3256..3322#3416..3499#3700..3794#4111..4175#4288..4486#5110..5304#5550..5644#5743..5878#5949..6166#6413..6540#6832..7301#aaagaagggaaaaaaaatacgaaaagcaaaaaggaataaacggagggggctaaacaccaaaattccccctctccttctccttcccctcctcccgaaaccctcgccgccgtcgctgtcgaaaccctcgctgctgctgctgccgcacggcgcggcggggtaccgggtccccctccctgcggcggcgcggaggggtaggtctcccccccctcgctgcggcgtggcggtggggggtgcggtgccggctgccgcgccatgatcttgcgccggtggactgcgcctatccaatgtgagctctcctcctacttccgccgcggcattagggttcttgcttcttgagctggaagagttctcttgcgttgcgaacgcctactgtttcctgcgaattcttagtgcttgttcttggattctttccgggggtgttcttaatctgttgtttgcaaaagttctttttttctttttttggctagggttttgaaatttgcaatcttggtgttcttttccctgagtttttactaaaaagctgcggcttttacgcaattcggtggtagtccaaagccgcgtcctttttttctagtctttacgcgcatttggctccaatttttctcctttttttagacgcgttttcttcacgctaatattcgcaactgaaatcttagacctaacgaaccattctaaaattgatgatcgttacattctttctgcagaaaaaaaaaagataaagatagaacaagtaggttactttccgtaccatggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggtatgcgtaagattcttcatgtggattccctgttcttactgttagcttattcgttattgtgaaatctgatagtttttctttttagtttgttgtcataaacactagaagtctcaaatgttgtatatgtggttctctgaaagcaaaatactcaaatgctgaagtaagtgcctcgcacagtcgcactccccaaaataccaggcaatgagacttgggacttaactgccaatttctgacttctgagggtactgcagacctatttttagcaccaagttatatcttgagtggtttgtgtccaagaactgtaactagaaaccaaaacaactagtttgataagttcttgatcaagcatactatgcaagagataaaactgtcgagataatcactcaagaattttccagcagaagattttttattatctccatgacatacttattagcaccgtaaaattttgtgcgtagctggtggatagaatgacatgtgaatgagctaaggctcagctagagctgcaaaaagagtaatacttttgccccttggagaatagtggtccctattaattggcagggttgtaaaaatcctaagttcctaaccaatctaacatataagatcaaaacagttggtcagtccaaaaattctctcggaaaagcttggaatttcgacattgtttctgtatttattatgtgatatatttatccataaatgttctgatggagagaaattgtaacaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcggtaccatcaccagctataatttatatctagatctctattattttgtttttgttatccatttaagttttttaatgtgttcatctgattaatgtagcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtaagctttattttttctgctcatacaaattatgctgattcccaaagtactgttgaaaactaatgttgacttatctttactgtatcccaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtgtggtcttgctaccttttgtttcaaccaaatcaacacattgcttcttgtggtgtcctgcatgtcaatcataatggttgccatcacttgcccatcatattagcagtcatgcgtgacaggcttgaactaagactagtgttgatttgaaaattgtctcaatcatgtgggttaatgaggaaacatcatgataactctatgttttttcgttgcttgtccgctcactttttactctgagcatagcaactctggcaatataattaattttgactaaccttagaatgaatcatatgaaatagacctttttaatttatactttggtatacctttaagaattgttaactctggcatttggtggtgatttttgtatttttctcttatcaattgacatttttcacaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggtcagtcataagaaaaaaatctttaatattctgtacataatgtcctctcttttgctgacactcttttctgttttccaaaggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaagtaagtggaactaattatgcagtatgcaatatctgcagatagtcacaagtatcacaacagattattcattatcttctattctgttgtagatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccaggcatgctcctctgctagtcttgcacaatttctattgggattggatgattgtatcatgttttagcttattgaatatgttgttcttcatatatagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtaatgtttaaccaaccaacatcatgagagcctcttaaattctgccatgtaatttgaaacagcattttccagctgagactcataacaactatataagcactaatcagtaattcatttcattgctttgcataattctctttttgatagctggaatgagactttaatacgtaactgacaatccatcactgctttcaccataggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcaggtactttctttgtcctaagcttgctaattaaaacttgtgtaatctttctgtgttgcatggagaattaaatttatttaagcaggtacagctaataattatacttatccatttagtagcaaactgggaagcatataaatgtttcttccttgtataaatagacaaatacttgtctattgcatgttcataatttgttccaaccttgatgttgcttgcccacggtatctgttttgctgctttttaaaaaaaaatatatatttgtggaatagctatggtttatatttgtaacattcaacttacgatatagcttgttctgcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacaggtcatttagcactttttcacaatatacagtcatatttatgttagattgttttttctgtttttatgtgttagaaattctcaactatatattcactgtattttatattttgcagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggtagatatagccgttccttttcttgtctctattttcctgcattttcacattgtgtcccaaaaaacttgtttagactgtcttgcctcattaagatttaaaatatatatggaaataatttatgcaaaaatattccttttgaaaacagaaatggtagttcaatggtcaataggatagtgaactgtaattatttttcaagataggcctctagactatgtccagtttcaggattaccactttattattgcctagactgaaatgttttaaatagtgggctaagacatttagtggttagccttctcaaacagctatagctatagcggctaaattgtacataagagcaaatatttagctagacccttctcaaacagctatagccggagatttaaaatgcatatcctgaaaccccccaagaaagattatcccagtaacagcgaatatataattggaaatcatccaaggtggctgacaactcggggccatttatttgtgacatatcagttattttccaacttgcatgtttcactacttgtacaaagaaattaggcccaatttatctgcaatgtgttcgagtacctgttttaatcacagttcattcatgttttcttcaattgaaaattatttaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggtacctttgtgcccatgattcataactgtcacgttacctaatttcagaactatttggacagaagtgatatcatcagatcattatgttctgaaaccagcttcttctgatcttgctaacatgattgcttccttttcactctagtcaatgaaactattcaagtgtctaataataaatgattatctgactattctactatgtgattagtgaactgacaggggcttcttttacttgaattgcctgcacaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagtaagacatctgcttttcttgcggtgatagctttctggcatgcacttttatacccacacatctagaacgtgacggtgacaattatgaattatctgcaggcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaaggtgacacataaaacttgagtaatcagttcatttttactggatcactatttattgatttttcttttggcagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataaggtcagttatttagatacttcctccgtttcatattataagactttctagcattgtccacattcatatagatgttgtgtctagatttattaacatctatgtgaatgtggacaatgctagaaagtcttataatatgaaacggagggagtacatcttttatcctatgtttaaacatgttatgtaaacgagtagatccattctactgcattgtatcttaaattgtctgaactttggttggttcctttgcagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttggtacgtgtctatttactctgtggtgtatgtagcaatagatcgaattgctgtacagttttattttcatgctatttgttgatcgacttgttttcttcttttgcatcgccatgtgaagactcggaaaaaaaatcactattgtgctgtctggttttgagttttgatacatataacttgatgattggagcaggaattctagctattgaatctgacctttctttacacagttgaatcaggtcgggtatatgttctccttgccgaattgttggttgacatttactgttattcttgcagggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtgaccaaggggagtcgagcatactccattgatgtgggaaacactgacccactgtagcttgagatgcaccattgctgtggtggacatttgttcttacagaattcgacgctttgcaggcctacactgggtgtctcatttgtctgacacccacaatggataagaaagaagagcacttctgtgccatccgacggtgatttctctggttacactttgacctttgtagcaaatgtaagattgatgggggatatgcttatagagtttgatttgtagaggtataccccctgtgtaggtttatgaacatgatggcttcagttgccatatgttagaagaaacagagaaagaggaggaagcccttgttgtaaatgcagaaaaaacagaactgaaaaactactagaatcaattgacggcggcatttcaacaggctgtgtcatgatg</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001069036.1 RefSeq:Os08g0559300]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 8]]
[[Category:Chromosome 8]]

Os08g0559300

2014-05-23T01:41:10Z

Haidongli: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==
===Function===
SPINDLY(SPY) encodes an O-linked N-acetylglucosamine transferase that is considered to be a negative regulator of gibberellin (GA) signaling through an unknown mechanism(Jacobsen and Olszewski,1993; Jacobsenet al., 1996;Swain et al., 2001).Shimada et al(2006) isolated a rice SPINDLY homolog (OsSPY) and produced knockdown transgenic plants in which OsSPY expression was reduced by introducing its antisense or RNAi construct;they find that in knockdown plants, the enhanced elongation of lower internodes was correlated with decreased levels of OsSPY expression, similar to the spindly phenotype of Arabidopsis spy mutants,The suppression of OsSPY function in a GA‐insensitive mutant, gid2, also caused an increase in the phosphorylation of a rice DELLA protein, SLR1, but did not change the amount of SLR1.suggest that OsSPY functions as a negative factor in GA signaling in rice; OsSPY in GA signaling is not via changes in the amount or stability of SLR1, but probably involves control of the suppressive function of SLR1;and OsSPY may play roles both in GA signaling and in the brassinosteroid pathway.

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
1.Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, Aichi 464-8601, Japan
2.Field Production Science Center, University of Tokyo, Nishi-Tokyo, Tokyo 188-0002, Japan
3.RIKEN (The Institute of Physical and Chemical Research), Wako-shi, Saitama 351-0198, Japan
4.Department of Chemistry, Joetsu University of Education, Joetsu-shi, Niigata 943-8512, Japan
5.Science and Technology Research Institute,Chiang Mai University,Chiang Mai 50200,Thailand
6.Molecular Biology Laboratory,Department of Biology, Faculty of Science, Chiang Mai University,Chiang Mai 50200,Thailand
7.Plasma and Beam Physics Research Facility,Department of Physics and Materials Science,Faculty of Science,Chiang Mai University,Chiang Mai 50200
8.Thailand Center of Excellence in Physics,Commission on Higher Education, 328 Si Ayutthaya Road,Bangkok 10400,Thailand

==References==
Jacobsen, S.E. and Olszewski, N.E.(1993) Mutations at theS PINDLY locus of Arabidopsisalter gibberellin signal transduction.Plant Cell, 5, 887–896.
Jacobsen, S.E., Binkowski, K.A. and Olszewski, N.E.(1996) SPINDLY, a tetratricopeptide repeat protein involved in gibberellin signal transduction in Arabidopsis. Proc. Natl Acad. Sci. USA, 93, 9292–9296.
Swain, S.M., Tseng, T.-S. and Olszewski, N.E. (2001) Altered expression ofSPINDLYaffects gibberellin response and plant development.Plant Physiol.126, 1174–1185.
Thornton, T.M., Swain, S.M. and Olszewski, N.E.(1999) Gibberellin signal transduction presents…the SPY who O-GlcNAc’d me.Trends Plant Sci.4, 424–428.

==Structured Information==
{{JaponicaGene|
GeneName = Os08g0559300|
Description = Similar to Gibberellin action negative regulator SPY|
Version = NM_001069036.1 GI:115477810 GeneID:4346315|
Length = 7732 bp|
Definition = Oryza sativa Japonica Group Os08g0559300, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 8|Chromosome 8]]|
AP = Chromosome 8:28079564..28087295|
CDS = 28080313..28080681,28081406..28081567,28081662..28081837,28081928..28082003,28082401..28082529 ,28082610..28082729,28082819..28082885,28082979..28083062,28083263..28083357 ,28083674..28083738,28083851..28084049,28084673..28084867,28085113..28085207 ,28085306..28085441,28085512..28085729,28085976..28086103,28086395..28086864 |
GCID = <gbrowseImage1>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcgcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaaatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttgggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtga</cdnaseq>|
AA = <aaseq>MGRPGMDSSEGRESNGVVPERNGGAVPAKQQLDGKDTLRYANIL RSRNKFAEALQLYNNVLEKDEANVEALIGKGICLQAQSLPMQAIECFNEAVRIDPGNA CALTYCGMIYKDEGHLVEAAEAYQKARNADPSYKPAAEFLAIVLTDLGTSLKLAGNTE EGIQKYCEALEVDSHYAPAYYNLGVVYSEMMQFDLALTCYEKAALERPLYAEAYCNMG VIYKNRGELEAAIACYERCLTISPNFEIAKNNMAIALTDLGTKVKIEGDINQGVAYYK KALFYNWHYADAMYNLGVAYGEMLNFEMAIVFYELALHFNPRCAEACNNLGVIYKDRD NLDKAVECYQMALSIKPNFSQSLNNLGVVYTVQGKMDAASSMIQKAIFANSTYAEAYN NLGVLYRDAGSITSAVQAYEKCLQIDPDSRNAGQNRLLALNYIDEGFDDKLYQAHREW GKRFLKLYPQYTSWDNPKVADRPLVIGYVSPDYFTHSVSYFIEAPLAHHDYSNYKVVV YSGVVKADAKTLRFKDKVLKKGGLWRDIYGIDEKKVASLVREDKVDILVELTGHTANN KLGTMACRPAPIQVTWIGYPNTTGLPTIDYRITDSLADPPDTTQKHVEELVRLPESFL CYSPSPEAGPVCPTPAILNGFITFGSFNNLAKITPKVLQVWAKILCAVPNSRLVVKCK PFCCDSIRQKFLSTLAELGLEPLRVDLLPLIHLNHDHMQAYSLMDISLDTFPYAGTTT TCESLYMGVPCVTMAGSVHAHNVGVSLLTKVGLGRLVAKSENEYVSLALDLAADVTAL QELRMSLRGLMAKSPVCDGENFTRGLESAYRNMWRRYCDGDAPALRRLDLLQEEPCSN NNKQDFDDNQVAKLADLKAQRVDAAVDGDKQSQLTAHAAVVGEVQQAPIMVNGVSSPV SSGKVEANGHISR</aaseq>|
DNA = <dnaseqindica>750..1118#1843..2004#2099..2274#2365..2440#2838..2966#3047..3166#3256..3322#3416..3499#3700..3794#4111..4175#4288..4486#5110..5304#5550..5644#5743..5878#5949..6166#6413..6540#6832..7301#aaagaagggaaaaaaaatacgaaaagcaaaaaggaataaacggagggggctaaacaccaaaattccccctctccttctccttcccctcctcccgaaaccctcgccgccgtcgctgtcgaaaccctcgctgctgctgctgccgcacggcgcggcggggtaccgggtccccctccctgcggcggcgcggaggggtaggtctcccccccctcgctgcggcgtggcggtggggggtgcggtgccggctgccgcgccatgatcttgcgccggtggactgcgcctatccaatgtgagctctcctcctacttccgccgcggcattagggttcttgcttcttgagctggaagagttctcttgcgttgcgaacgcctactgtttcctgcgaattcttagtgcttgttcttggattctttccgggggtgttcttaatctgttgtttgcaaaagttctttttttctttttttggctagggttttgaaatttgcaatcttggtgttcttttccctgagtttttactaaaaagctgcggcttttacgcaattcggtggtagtccaaagccgcgtcctttttttctagtctttacgcgcatttggctccaatttttctcctttttttagacgcgttttcttcacgctaatattcgcaactgaaatcttagacctaacgaaccattctaaaattgatgatcgttacattctttctgcagaaaaaaaaaagataaagatagaacaagtaggttactttccgtaccatggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggtatgcgtaagattcttcatgtggattccctgttcttactgttagcttattcgttattgtgaaatctgatagtttttctttttagtttgttgtcataaacactagaagtctcaaatgttgtatatgtggttctctgaaagcaaaatactcaaatgctgaagtaagtgcctcgcacagtcgcactccccaaaataccaggcaatgagacttgggacttaactgccaatttctgacttctgagggtactgcagacctatttttagcaccaagttatatcttgagtggtttgtgtccaagaactgtaactagaaaccaaaacaactagtttgataagttcttgatcaagcatactatgcaagagataaaactgtcgagataatcactcaagaattttccagcagaagattttttattatctccatgacatacttattagcaccgtaaaattttgtgcgtagctggtggatagaatgacatgtgaatgagctaaggctcagctagagctgcaaaaagagtaatacttttgccccttggagaatagtggtccctattaattggcagggttgtaaaaatcctaagttcctaaccaatctaacatataagatcaaaacagttggtcagtccaaaaattctctcggaaaagcttggaatttcgacattgtttctgtatttattatgtgatatatttatccataaatgttctgatggagagaaattgtaacaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcggtaccatcaccagctataatttatatctagatctctattattttgtttttgttatccatttaagttttttaatgtgttcatctgattaatgtagcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtaagctttattttttctgctcatacaaattatgctgattcccaaagtactgttgaaaactaatgttgacttatctttactgtatcccaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtgtggtcttgctaccttttgtttcaaccaaatcaacacattgcttcttgtggtgtcctgcatgtcaatcataatggttgccatcacttgcccatcatattagcagtcatgcgtgacaggcttgaactaagactagtgttgatttgaaaattgtctcaatcatgtgggttaatgaggaaacatcatgataactctatgttttttcgttgcttgtccgctcactttttactctgagcatagcaactctggcaatataattaattttgactaaccttagaatgaatcatatgaaatagacctttttaatttatactttggtatacctttaagaattgttaactctggcatttggtggtgatttttgtatttttctcttatcaattgacatttttcacaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggtcagtcataagaaaaaaatctttaatattctgtacataatgtcctctcttttgctgacactcttttctgttttccaaaggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaagtaagtggaactaattatgcagtatgcaatatctgcagatagtcacaagtatcacaacagattattcattatcttctattctgttgtagatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccaggcatgctcctctgctagtcttgcacaatttctattgggattggatgattgtatcatgttttagcttattgaatatgttgttcttcatatatagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtaatgtttaaccaaccaacatcatgagagcctcttaaattctgccatgtaatttgaaacagcattttccagctgagactcataacaactatataagcactaatcagtaattcatttcattgctttgcataattctctttttgatagctggaatgagactttaatacgtaactgacaatccatcactgctttcaccataggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcaggtactttctttgtcctaagcttgctaattaaaacttgtgtaatctttctgtgttgcatggagaattaaatttatttaagcaggtacagctaataattatacttatccatttagtagcaaactgggaagcatataaatgtttcttccttgtataaatagacaaatacttgtctattgcatgttcataatttgttccaaccttgatgttgcttgcccacggtatctgttttgctgctttttaaaaaaaaatatatatttgtggaatagctatggtttatatttgtaacattcaacttacgatatagcttgttctgcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacaggtcatttagcactttttcacaatatacagtcatatttatgttagattgttttttctgtttttatgtgttagaaattctcaactatatattcactgtattttatattttgcagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggtagatatagccgttccttttcttgtctctattttcctgcattttcacattgtgtcccaaaaaacttgtttagactgtcttgcctcattaagatttaaaatatatatggaaataatttatgcaaaaatattccttttgaaaacagaaatggtagttcaatggtcaataggatagtgaactgtaattatttttcaagataggcctctagactatgtccagtttcaggattaccactttattattgcctagactgaaatgttttaaatagtgggctaagacatttagtggttagccttctcaaacagctatagctatagcggctaaattgtacataagagcaaatatttagctagacccttctcaaacagctatagccggagatttaaaatgcatatcctgaaaccccccaagaaagattatcccagtaacagcgaatatataattggaaatcatccaaggtggctgacaactcggggccatttatttgtgacatatcagttattttccaacttgcatgtttcactacttgtacaaagaaattaggcccaatttatctgcaatgtgttcgagtacctgttttaatcacagttcattcatgttttcttcaattgaaaattatttaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggtacctttgtgcccatgattcataactgtcacgttacctaatttcagaactatttggacagaagtgatatcatcagatcattatgttctgaaaccagcttcttctgatcttgctaacatgattgcttccttttcactctagtcaatgaaactattcaagtgtctaataataaatgattatctgactattctactatgtgattagtgaactgacaggggcttcttttacttgaattgcctgcacaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagtaagacatctgcttttcttgcggtgatagctttctggcatgcacttttatacccacacatctagaacgtgacggtgacaattatgaattatctgcaggcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaaggtgacacataaaacttgagtaatcagttcatttttactggatcactatttattgatttttcttttggcagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataaggtcagttatttagatacttcctccgtttcatattataagactttctagcattgtccacattcatatagatgttgtgtctagatttattaacatctatgtgaatgtggacaatgctagaaagtcttataatatgaaacggagggagtacatcttttatcctatgtttaaacatgttatgtaaacgagtagatccattctactgcattgtatcttaaattgtctgaactttggttggttcctttgcagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttggtacgtgtctatttactctgtggtgtatgtagcaatagatcgaattgctgtacagttttattttcatgctatttgttgatcgacttgttttcttcttttgcatcgccatgtgaagactcggaaaaaaaatcactattgtgctgtctggttttgagttttgatacatataacttgatgattggagcaggaattctagctattgaatctgacctttctttacacagttgaatcaggtcgggtatatgttctccttgccgaattgttggttgacatttactgttattcttgcagggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtgaccaaggggagtcgagcatactccattgatgtgggaaacactgacccactgtagcttgagatgcaccattgctgtggtggacatttgttcttacagaattcgacgctttgcaggcctacactgggtgtctcatttgtctgacacccacaatggataagaaagaagagcacttctgtgccatccgacggtgatttctctggttacactttgacctttgtagcaaatgtaagattgatgggggatatgcttatagagtttgatttgtagaggtataccccctgtgtaggtttatgaacatgatggcttcagttgccatatgttagaagaaacagagaaagaggaggaagcccttgttgtaaatgcagaaaaaacagaactgaaaaactactagaatcaattgacggcggcatttcaacaggctgtgtcatgatg</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001069036.1 RefSeq:Os08g0559300]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 8]]
[[Category:Chromosome 8]]

Os08g0559300

2014-05-23T01:33:31Z

Haidongli: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==
===Function===
SPINDLY(SPY) encodes an O-linked N-acetylglucosamine transferase that is considered to be a negative regulator of gibberellin (GA) signaling through an unknown mechanism(Jacobsen and Olszewski,1993; Jacobsenet al., 1996;Swain et al., 2001).Shimada et al(2006) isolated a rice SPINDLY homolog (OsSPY) and produced knockdown transgenic plants in which OsSPY expression was reduced by introducing its antisense or RNAi construct;they find that in knockdown plants, the enhanced elongation of lower internodes was correlated with decreased levels of OsSPY expression, similar to the spindly phenotype of Arabidopsis spy mutants,The suppression of OsSPY function in a GA‐insensitive mutant, gid2, also caused an increase in the phosphorylation of a rice DELLA protein, SLR1, but did not change the amount of SLR1.suggest that OsSPY functions as a negative factor in GA signaling in rice; OsSPY in GA signaling is not via changes in the amount or stability of SLR1, but probably involves control of the suppressive function of SLR1;and OsSPY may play roles both in GA signaling and in the brassinosteroid pathway.

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
1.Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, Aichi 464-8601, Japan
2.Field Production Science Center, University of Tokyo, Nishi-Tokyo, Tokyo 188-0002, Japan
3.RIKEN (The Institute of Physical and Chemical Research), Wako-shi, Saitama 351-0198, Japan
4.Department of Chemistry, Joetsu University of Education, Joetsu-shi, Niigata 943-8512, Japan
5.Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand
6.Molecular Biology Laboratory, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand
7.Plasma and Beam Physics Research Facility, Department of Physics and Materials Science, Faculty of Science, Chiang Mai University,
Chiang Mai 50200, Thailand
8.Thailand Center of Excellence in Physics, Commission on Higher Education, 328 Si Ayutthaya Road, Bangkok 10400, Thailand

==References==
Jacobsen, S.E. and Olszewski, N.E.(1993) Mutations at theS PINDLY locus of Arabidopsisalter gibberellin signal transduction.Plant Cell, 5, 887–896.
Jacobsen, S.E., Binkowski, K.A. and Olszewski, N.E.(1996) SPINDLY, a tetratricopeptide repeat protein involved in gibberellin signal transduction in Arabidopsis. Proc. Natl Acad. Sci. USA, 93, 9292–9296.
Swain, S.M., Tseng, T.-S. and Olszewski, N.E. (2001) Altered expression ofSPINDLYaffects gibberellin response and plant development.Plant Physiol.126, 1174–1185.
Thornton, T.M., Swain, S.M. and Olszewski, N.E.(1999) Gibberellin signal transduction presents…the SPY who O-GlcNAc’d me.Trends Plant Sci.4, 424–428.

==Structured Information==
{{JaponicaGene|
GeneName = Os08g0559300|
Description = Similar to Gibberellin action negative regulator SPY|
Version = NM_001069036.1 GI:115477810 GeneID:4346315|
Length = 7732 bp|
Definition = Oryza sativa Japonica Group Os08g0559300, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 8|Chromosome 8]]|
AP = Chromosome 8:28079564..28087295|
CDS = 28080313..28080681,28081406..28081567,28081662..28081837,28081928..28082003,28082401..28082529 ,28082610..28082729,28082819..28082885,28082979..28083062,28083263..28083357 ,28083674..28083738,28083851..28084049,28084673..28084867,28085113..28085207 ,28085306..28085441,28085512..28085729,28085976..28086103,28086395..28086864 |
GCID = <gbrowseImage1>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcgcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaaatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttgggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtga</cdnaseq>|
AA = <aaseq>MGRPGMDSSEGRESNGVVPERNGGAVPAKQQLDGKDTLRYANIL RSRNKFAEALQLYNNVLEKDEANVEALIGKGICLQAQSLPMQAIECFNEAVRIDPGNA CALTYCGMIYKDEGHLVEAAEAYQKARNADPSYKPAAEFLAIVLTDLGTSLKLAGNTE EGIQKYCEALEVDSHYAPAYYNLGVVYSEMMQFDLALTCYEKAALERPLYAEAYCNMG VIYKNRGELEAAIACYERCLTISPNFEIAKNNMAIALTDLGTKVKIEGDINQGVAYYK KALFYNWHYADAMYNLGVAYGEMLNFEMAIVFYELALHFNPRCAEACNNLGVIYKDRD NLDKAVECYQMALSIKPNFSQSLNNLGVVYTVQGKMDAASSMIQKAIFANSTYAEAYN NLGVLYRDAGSITSAVQAYEKCLQIDPDSRNAGQNRLLALNYIDEGFDDKLYQAHREW GKRFLKLYPQYTSWDNPKVADRPLVIGYVSPDYFTHSVSYFIEAPLAHHDYSNYKVVV YSGVVKADAKTLRFKDKVLKKGGLWRDIYGIDEKKVASLVREDKVDILVELTGHTANN KLGTMACRPAPIQVTWIGYPNTTGLPTIDYRITDSLADPPDTTQKHVEELVRLPESFL CYSPSPEAGPVCPTPAILNGFITFGSFNNLAKITPKVLQVWAKILCAVPNSRLVVKCK PFCCDSIRQKFLSTLAELGLEPLRVDLLPLIHLNHDHMQAYSLMDISLDTFPYAGTTT TCESLYMGVPCVTMAGSVHAHNVGVSLLTKVGLGRLVAKSENEYVSLALDLAADVTAL QELRMSLRGLMAKSPVCDGENFTRGLESAYRNMWRRYCDGDAPALRRLDLLQEEPCSN NNKQDFDDNQVAKLADLKAQRVDAAVDGDKQSQLTAHAAVVGEVQQAPIMVNGVSSPV SSGKVEANGHISR</aaseq>|
DNA = <dnaseqindica>750..1118#1843..2004#2099..2274#2365..2440#2838..2966#3047..3166#3256..3322#3416..3499#3700..3794#4111..4175#4288..4486#5110..5304#5550..5644#5743..5878#5949..6166#6413..6540#6832..7301#aaagaagggaaaaaaaatacgaaaagcaaaaaggaataaacggagggggctaaacaccaaaattccccctctccttctccttcccctcctcccgaaaccctcgccgccgtcgctgtcgaaaccctcgctgctgctgctgccgcacggcgcggcggggtaccgggtccccctccctgcggcggcgcggaggggtaggtctcccccccctcgctgcggcgtggcggtggggggtgcggtgccggctgccgcgccatgatcttgcgccggtggactgcgcctatccaatgtgagctctcctcctacttccgccgcggcattagggttcttgcttcttgagctggaagagttctcttgcgttgcgaacgcctactgtttcctgcgaattcttagtgcttgttcttggattctttccgggggtgttcttaatctgttgtttgcaaaagttctttttttctttttttggctagggttttgaaatttgcaatcttggtgttcttttccctgagtttttactaaaaagctgcggcttttacgcaattcggtggtagtccaaagccgcgtcctttttttctagtctttacgcgcatttggctccaatttttctcctttttttagacgcgttttcttcacgctaatattcgcaactgaaatcttagacctaacgaaccattctaaaattgatgatcgttacattctttctgcagaaaaaaaaaagataaagatagaacaagtaggttactttccgtaccatggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggtatgcgtaagattcttcatgtggattccctgttcttactgttagcttattcgttattgtgaaatctgatagtttttctttttagtttgttgtcataaacactagaagtctcaaatgttgtatatgtggttctctgaaagcaaaatactcaaatgctgaagtaagtgcctcgcacagtcgcactccccaaaataccaggcaatgagacttgggacttaactgccaatttctgacttctgagggtactgcagacctatttttagcaccaagttatatcttgagtggtttgtgtccaagaactgtaactagaaaccaaaacaactagtttgataagttcttgatcaagcatactatgcaagagataaaactgtcgagataatcactcaagaattttccagcagaagattttttattatctccatgacatacttattagcaccgtaaaattttgtgcgtagctggtggatagaatgacatgtgaatgagctaaggctcagctagagctgcaaaaagagtaatacttttgccccttggagaatagtggtccctattaattggcagggttgtaaaaatcctaagttcctaaccaatctaacatataagatcaaaacagttggtcagtccaaaaattctctcggaaaagcttggaatttcgacattgtttctgtatttattatgtgatatatttatccataaatgttctgatggagagaaattgtaacaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcggtaccatcaccagctataatttatatctagatctctattattttgtttttgttatccatttaagttttttaatgtgttcatctgattaatgtagcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtaagctttattttttctgctcatacaaattatgctgattcccaaagtactgttgaaaactaatgttgacttatctttactgtatcccaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtgtggtcttgctaccttttgtttcaaccaaatcaacacattgcttcttgtggtgtcctgcatgtcaatcataatggttgccatcacttgcccatcatattagcagtcatgcgtgacaggcttgaactaagactagtgttgatttgaaaattgtctcaatcatgtgggttaatgaggaaacatcatgataactctatgttttttcgttgcttgtccgctcactttttactctgagcatagcaactctggcaatataattaattttgactaaccttagaatgaatcatatgaaatagacctttttaatttatactttggtatacctttaagaattgttaactctggcatttggtggtgatttttgtatttttctcttatcaattgacatttttcacaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggtcagtcataagaaaaaaatctttaatattctgtacataatgtcctctcttttgctgacactcttttctgttttccaaaggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaagtaagtggaactaattatgcagtatgcaatatctgcagatagtcacaagtatcacaacagattattcattatcttctattctgttgtagatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccaggcatgctcctctgctagtcttgcacaatttctattgggattggatgattgtatcatgttttagcttattgaatatgttgttcttcatatatagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtaatgtttaaccaaccaacatcatgagagcctcttaaattctgccatgtaatttgaaacagcattttccagctgagactcataacaactatataagcactaatcagtaattcatttcattgctttgcataattctctttttgatagctggaatgagactttaatacgtaactgacaatccatcactgctttcaccataggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcaggtactttctttgtcctaagcttgctaattaaaacttgtgtaatctttctgtgttgcatggagaattaaatttatttaagcaggtacagctaataattatacttatccatttagtagcaaactgggaagcatataaatgtttcttccttgtataaatagacaaatacttgtctattgcatgttcataatttgttccaaccttgatgttgcttgcccacggtatctgttttgctgctttttaaaaaaaaatatatatttgtggaatagctatggtttatatttgtaacattcaacttacgatatagcttgttctgcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacaggtcatttagcactttttcacaatatacagtcatatttatgttagattgttttttctgtttttatgtgttagaaattctcaactatatattcactgtattttatattttgcagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggtagatatagccgttccttttcttgtctctattttcctgcattttcacattgtgtcccaaaaaacttgtttagactgtcttgcctcattaagatttaaaatatatatggaaataatttatgcaaaaatattccttttgaaaacagaaatggtagttcaatggtcaataggatagtgaactgtaattatttttcaagataggcctctagactatgtccagtttcaggattaccactttattattgcctagactgaaatgttttaaatagtgggctaagacatttagtggttagccttctcaaacagctatagctatagcggctaaattgtacataagagcaaatatttagctagacccttctcaaacagctatagccggagatttaaaatgcatatcctgaaaccccccaagaaagattatcccagtaacagcgaatatataattggaaatcatccaaggtggctgacaactcggggccatttatttgtgacatatcagttattttccaacttgcatgtttcactacttgtacaaagaaattaggcccaatttatctgcaatgtgttcgagtacctgttttaatcacagttcattcatgttttcttcaattgaaaattatttaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggtacctttgtgcccatgattcataactgtcacgttacctaatttcagaactatttggacagaagtgatatcatcagatcattatgttctgaaaccagcttcttctgatcttgctaacatgattgcttccttttcactctagtcaatgaaactattcaagtgtctaataataaatgattatctgactattctactatgtgattagtgaactgacaggggcttcttttacttgaattgcctgcacaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagtaagacatctgcttttcttgcggtgatagctttctggcatgcacttttatacccacacatctagaacgtgacggtgacaattatgaattatctgcaggcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaaggtgacacataaaacttgagtaatcagttcatttttactggatcactatttattgatttttcttttggcagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataaggtcagttatttagatacttcctccgtttcatattataagactttctagcattgtccacattcatatagatgttgtgtctagatttattaacatctatgtgaatgtggacaatgctagaaagtcttataatatgaaacggagggagtacatcttttatcctatgtttaaacatgttatgtaaacgagtagatccattctactgcattgtatcttaaattgtctgaactttggttggttcctttgcagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttggtacgtgtctatttactctgtggtgtatgtagcaatagatcgaattgctgtacagttttattttcatgctatttgttgatcgacttgttttcttcttttgcatcgccatgtgaagactcggaaaaaaaatcactattgtgctgtctggttttgagttttgatacatataacttgatgattggagcaggaattctagctattgaatctgacctttctttacacagttgaatcaggtcgggtatatgttctccttgccgaattgttggttgacatttactgttattcttgcagggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtgaccaaggggagtcgagcatactccattgatgtgggaaacactgacccactgtagcttgagatgcaccattgctgtggtggacatttgttcttacagaattcgacgctttgcaggcctacactgggtgtctcatttgtctgacacccacaatggataagaaagaagagcacttctgtgccatccgacggtgatttctctggttacactttgacctttgtagcaaatgtaagattgatgggggatatgcttatagagtttgatttgtagaggtataccccctgtgtaggtttatgaacatgatggcttcagttgccatatgttagaagaaacagagaaagaggaggaagcccttgttgtaaatgcagaaaaaacagaactgaaaaactactagaatcaattgacggcggcatttcaacaggctgtgtcatgatg</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001069036.1 RefSeq:Os08g0559300]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 8]]
[[Category:Chromosome 8]]

Os08g0559300

2014-05-23T01:31:38Z

Haidongli: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==
===Function===
SPINDLY(SPY) encodes an O-linked N-acetylglucosamine transferase that is considered to be a negative regulator of gibberellin (GA) signaling through an unknown mechanism(Jacobsen and Olszewski,1993; Jacobsenet al., 1996;Swain et al., 2001).Shimada et al(2006) isolated a rice SPINDLY homolog (OsSPY) and produced knockdown transgenic plants in which OsSPY expression was reduced by introducing its antisense or RNAi construct;they find that in knockdown plants, the enhanced elongation of lower internodes was correlated with decreased levels of OsSPY expression, similar to the spindly phenotype of Arabidopsis spy mutants,The suppression of OsSPY function in a GA‐insensitive mutant, gid2, also caused an increase in the phosphorylation of a rice DELLA protein, SLR1, but did not change the amount of SLR1.suggest that OsSPY functions as a negative factor in GA signaling in rice; OsSPY in GA signaling is not via changes in the amount or stability of SLR1, but probably involves control of the suppressive function of SLR1;and OsSPY may play roles both in GA signaling and in the brassinosteroid pathway.

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
[[1.Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, Aichi 464-8601, Japan;
2.Field Production Science Center, University of Tokyo, Nishi-Tokyo, Tokyo 188-0002, Japan;
3.RIKEN (The Institute of Physical and Chemical Research), Wako-shi, Saitama 351-0198, Japan;
4.Department of Chemistry, Joetsu University of Education, Joetsu-shi, Niigata 943-8512, Japan;
5.Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand;
6.Molecular Biology Laboratory, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand;
7.Plasma and Beam Physics Research Facility, Department of Physics and Materials Science, Faculty of Science, Chiang Mai University,
Chiang Mai 50200, Thailand;
8.Thailand Center of Excellence in Physics, Commission on Higher Education, 328 Si Ayutthaya Road, Bangkok 10400, Thailand
]]

==References==
Jacobsen, S.E. and Olszewski, N.E.(1993) Mutations at theS PINDLY locus of Arabidopsisalter gibberellin signal transduction.Plant Cell, 5, 887–896.
Jacobsen, S.E., Binkowski, K.A. and Olszewski, N.E.(1996) SPINDLY, a tetratricopeptide repeat protein involved in gibberellin signal transduction in Arabidopsis. Proc. Natl Acad. Sci. USA, 93, 9292–9296.
Swain, S.M., Tseng, T.-S. and Olszewski, N.E. (2001) Altered expression ofSPINDLYaffects gibberellin response and plant development.Plant Physiol.126, 1174–1185.
Thornton, T.M., Swain, S.M. and Olszewski, N.E.(1999) Gibberellin signal transduction presents…the SPY who O-GlcNAc’d me.Trends Plant Sci.4, 424–428.

==Structured Information==
{{JaponicaGene|
GeneName = Os08g0559300|
Description = Similar to Gibberellin action negative regulator SPY|
Version = NM_001069036.1 GI:115477810 GeneID:4346315|
Length = 7732 bp|
Definition = Oryza sativa Japonica Group Os08g0559300, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 8|Chromosome 8]]|
AP = Chromosome 8:28079564..28087295|
CDS = 28080313..28080681,28081406..28081567,28081662..28081837,28081928..28082003,28082401..28082529 ,28082610..28082729,28082819..28082885,28082979..28083062,28083263..28083357 ,28083674..28083738,28083851..28084049,28084673..28084867,28085113..28085207 ,28085306..28085441,28085512..28085729,28085976..28086103,28086395..28086864 |
GCID = <gbrowseImage1>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcgcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaaatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttgggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtga</cdnaseq>|
AA = <aaseq>MGRPGMDSSEGRESNGVVPERNGGAVPAKQQLDGKDTLRYANIL RSRNKFAEALQLYNNVLEKDEANVEALIGKGICLQAQSLPMQAIECFNEAVRIDPGNA CALTYCGMIYKDEGHLVEAAEAYQKARNADPSYKPAAEFLAIVLTDLGTSLKLAGNTE EGIQKYCEALEVDSHYAPAYYNLGVVYSEMMQFDLALTCYEKAALERPLYAEAYCNMG VIYKNRGELEAAIACYERCLTISPNFEIAKNNMAIALTDLGTKVKIEGDINQGVAYYK KALFYNWHYADAMYNLGVAYGEMLNFEMAIVFYELALHFNPRCAEACNNLGVIYKDRD NLDKAVECYQMALSIKPNFSQSLNNLGVVYTVQGKMDAASSMIQKAIFANSTYAEAYN NLGVLYRDAGSITSAVQAYEKCLQIDPDSRNAGQNRLLALNYIDEGFDDKLYQAHREW GKRFLKLYPQYTSWDNPKVADRPLVIGYVSPDYFTHSVSYFIEAPLAHHDYSNYKVVV YSGVVKADAKTLRFKDKVLKKGGLWRDIYGIDEKKVASLVREDKVDILVELTGHTANN KLGTMACRPAPIQVTWIGYPNTTGLPTIDYRITDSLADPPDTTQKHVEELVRLPESFL CYSPSPEAGPVCPTPAILNGFITFGSFNNLAKITPKVLQVWAKILCAVPNSRLVVKCK PFCCDSIRQKFLSTLAELGLEPLRVDLLPLIHLNHDHMQAYSLMDISLDTFPYAGTTT TCESLYMGVPCVTMAGSVHAHNVGVSLLTKVGLGRLVAKSENEYVSLALDLAADVTAL QELRMSLRGLMAKSPVCDGENFTRGLESAYRNMWRRYCDGDAPALRRLDLLQEEPCSN NNKQDFDDNQVAKLADLKAQRVDAAVDGDKQSQLTAHAAVVGEVQQAPIMVNGVSSPV SSGKVEANGHISR</aaseq>|
DNA = <dnaseqindica>750..1118#1843..2004#2099..2274#2365..2440#2838..2966#3047..3166#3256..3322#3416..3499#3700..3794#4111..4175#4288..4486#5110..5304#5550..5644#5743..5878#5949..6166#6413..6540#6832..7301#aaagaagggaaaaaaaatacgaaaagcaaaaaggaataaacggagggggctaaacaccaaaattccccctctccttctccttcccctcctcccgaaaccctcgccgccgtcgctgtcgaaaccctcgctgctgctgctgccgcacggcgcggcggggtaccgggtccccctccctgcggcggcgcggaggggtaggtctcccccccctcgctgcggcgtggcggtggggggtgcggtgccggctgccgcgccatgatcttgcgccggtggactgcgcctatccaatgtgagctctcctcctacttccgccgcggcattagggttcttgcttcttgagctggaagagttctcttgcgttgcgaacgcctactgtttcctgcgaattcttagtgcttgttcttggattctttccgggggtgttcttaatctgttgtttgcaaaagttctttttttctttttttggctagggttttgaaatttgcaatcttggtgttcttttccctgagtttttactaaaaagctgcggcttttacgcaattcggtggtagtccaaagccgcgtcctttttttctagtctttacgcgcatttggctccaatttttctcctttttttagacgcgttttcttcacgctaatattcgcaactgaaatcttagacctaacgaaccattctaaaattgatgatcgttacattctttctgcagaaaaaaaaaagataaagatagaacaagtaggttactttccgtaccatggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggtatgcgtaagattcttcatgtggattccctgttcttactgttagcttattcgttattgtgaaatctgatagtttttctttttagtttgttgtcataaacactagaagtctcaaatgttgtatatgtggttctctgaaagcaaaatactcaaatgctgaagtaagtgcctcgcacagtcgcactccccaaaataccaggcaatgagacttgggacttaactgccaatttctgacttctgagggtactgcagacctatttttagcaccaagttatatcttgagtggtttgtgtccaagaactgtaactagaaaccaaaacaactagtttgataagttcttgatcaagcatactatgcaagagataaaactgtcgagataatcactcaagaattttccagcagaagattttttattatctccatgacatacttattagcaccgtaaaattttgtgcgtagctggtggatagaatgacatgtgaatgagctaaggctcagctagagctgcaaaaagagtaatacttttgccccttggagaatagtggtccctattaattggcagggttgtaaaaatcctaagttcctaaccaatctaacatataagatcaaaacagttggtcagtccaaaaattctctcggaaaagcttggaatttcgacattgtttctgtatttattatgtgatatatttatccataaatgttctgatggagagaaattgtaacaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcggtaccatcaccagctataatttatatctagatctctattattttgtttttgttatccatttaagttttttaatgtgttcatctgattaatgtagcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtaagctttattttttctgctcatacaaattatgctgattcccaaagtactgttgaaaactaatgttgacttatctttactgtatcccaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtgtggtcttgctaccttttgtttcaaccaaatcaacacattgcttcttgtggtgtcctgcatgtcaatcataatggttgccatcacttgcccatcatattagcagtcatgcgtgacaggcttgaactaagactagtgttgatttgaaaattgtctcaatcatgtgggttaatgaggaaacatcatgataactctatgttttttcgttgcttgtccgctcactttttactctgagcatagcaactctggcaatataattaattttgactaaccttagaatgaatcatatgaaatagacctttttaatttatactttggtatacctttaagaattgttaactctggcatttggtggtgatttttgtatttttctcttatcaattgacatttttcacaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggtcagtcataagaaaaaaatctttaatattctgtacataatgtcctctcttttgctgacactcttttctgttttccaaaggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaagtaagtggaactaattatgcagtatgcaatatctgcagatagtcacaagtatcacaacagattattcattatcttctattctgttgtagatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccaggcatgctcctctgctagtcttgcacaatttctattgggattggatgattgtatcatgttttagcttattgaatatgttgttcttcatatatagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtaatgtttaaccaaccaacatcatgagagcctcttaaattctgccatgtaatttgaaacagcattttccagctgagactcataacaactatataagcactaatcagtaattcatttcattgctttgcataattctctttttgatagctggaatgagactttaatacgtaactgacaatccatcactgctttcaccataggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcaggtactttctttgtcctaagcttgctaattaaaacttgtgtaatctttctgtgttgcatggagaattaaatttatttaagcaggtacagctaataattatacttatccatttagtagcaaactgggaagcatataaatgtttcttccttgtataaatagacaaatacttgtctattgcatgttcataatttgttccaaccttgatgttgcttgcccacggtatctgttttgctgctttttaaaaaaaaatatatatttgtggaatagctatggtttatatttgtaacattcaacttacgatatagcttgttctgcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacaggtcatttagcactttttcacaatatacagtcatatttatgttagattgttttttctgtttttatgtgttagaaattctcaactatatattcactgtattttatattttgcagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggtagatatagccgttccttttcttgtctctattttcctgcattttcacattgtgtcccaaaaaacttgtttagactgtcttgcctcattaagatttaaaatatatatggaaataatttatgcaaaaatattccttttgaaaacagaaatggtagttcaatggtcaataggatagtgaactgtaattatttttcaagataggcctctagactatgtccagtttcaggattaccactttattattgcctagactgaaatgttttaaatagtgggctaagacatttagtggttagccttctcaaacagctatagctatagcggctaaattgtacataagagcaaatatttagctagacccttctcaaacagctatagccggagatttaaaatgcatatcctgaaaccccccaagaaagattatcccagtaacagcgaatatataattggaaatcatccaaggtggctgacaactcggggccatttatttgtgacatatcagttattttccaacttgcatgtttcactacttgtacaaagaaattaggcccaatttatctgcaatgtgttcgagtacctgttttaatcacagttcattcatgttttcttcaattgaaaattatttaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggtacctttgtgcccatgattcataactgtcacgttacctaatttcagaactatttggacagaagtgatatcatcagatcattatgttctgaaaccagcttcttctgatcttgctaacatgattgcttccttttcactctagtcaatgaaactattcaagtgtctaataataaatgattatctgactattctactatgtgattagtgaactgacaggggcttcttttacttgaattgcctgcacaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagtaagacatctgcttttcttgcggtgatagctttctggcatgcacttttatacccacacatctagaacgtgacggtgacaattatgaattatctgcaggcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaaggtgacacataaaacttgagtaatcagttcatttttactggatcactatttattgatttttcttttggcagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataaggtcagttatttagatacttcctccgtttcatattataagactttctagcattgtccacattcatatagatgttgtgtctagatttattaacatctatgtgaatgtggacaatgctagaaagtcttataatatgaaacggagggagtacatcttttatcctatgtttaaacatgttatgtaaacgagtagatccattctactgcattgtatcttaaattgtctgaactttggttggttcctttgcagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttggtacgtgtctatttactctgtggtgtatgtagcaatagatcgaattgctgtacagttttattttcatgctatttgttgatcgacttgttttcttcttttgcatcgccatgtgaagactcggaaaaaaaatcactattgtgctgtctggttttgagttttgatacatataacttgatgattggagcaggaattctagctattgaatctgacctttctttacacagttgaatcaggtcgggtatatgttctccttgccgaattgttggttgacatttactgttattcttgcagggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtgaccaaggggagtcgagcatactccattgatgtgggaaacactgacccactgtagcttgagatgcaccattgctgtggtggacatttgttcttacagaattcgacgctttgcaggcctacactgggtgtctcatttgtctgacacccacaatggataagaaagaagagcacttctgtgccatccgacggtgatttctctggttacactttgacctttgtagcaaatgtaagattgatgggggatatgcttatagagtttgatttgtagaggtataccccctgtgtaggtttatgaacatgatggcttcagttgccatatgttagaagaaacagagaaagaggaggaagcccttgttgtaaatgcagaaaaaacagaactgaaaaactactagaatcaattgacggcggcatttcaacaggctgtgtcatgatg</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001069036.1 RefSeq:Os08g0559300]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 8]]
[[Category:Chromosome 8]]

Os08g0559300

2014-05-23T01:30:48Z

Haidongli: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==
===Function===
SPINDLY(SPY) encodes an O-linked N-acetylglucosamine transferase that is considered to be a negative regulator of gibberellin (GA) signaling through an unknown mechanism(Jacobsen and Olszewski,1993; Jacobsenet al., 1996;Swain et al., 2001).Shimada et al(2006) isolated a rice SPINDLY homolog (OsSPY) and produced knockdown transgenic plants in which OsSPY expression was reduced by introducing its antisense or RNAi construct;they find that in knockdown plants, the enhanced elongation of lower internodes was correlated with decreased levels of OsSPY expression, similar to the spindly phenotype of Arabidopsis spy mutants,The suppression of OsSPY function in a GA‐insensitive mutant, gid2, also caused an increase in the phosphorylation of a rice DELLA protein, SLR1, but did not change the amount of SLR1.suggest that OsSPY functions as a negative factor in GA signaling in rice; OsSPY in GA signaling is not via changes in the amount or stability of SLR1, but probably involves control of the suppressive function of SLR1;and OsSPY may play roles both in GA signaling and in the brassinosteroid pathway.

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
1.Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, Aichi 464-8601, Japan;
2.Field Production Science Center, University of Tokyo, Nishi-Tokyo, Tokyo 188-0002, Japan;
3.RIKEN (The Institute of Physical and Chemical Research), Wako-shi, Saitama 351-0198, Japan;
4.Department of Chemistry, Joetsu University of Education, Joetsu-shi, Niigata 943-8512, Japan;
5.Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand;
6.Molecular Biology Laboratory, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand;
7.Plasma and Beam Physics Research Facility, Department of Physics and Materials Science, Faculty of Science, Chiang Mai University,
Chiang Mai 50200, Thailand;
8.Thailand Center of Excellence in Physics, Commission on Higher Education, 328 Si Ayutthaya Road, Bangkok 10400, Thailand

==References==
Jacobsen, S.E. and Olszewski, N.E.(1993) Mutations at theS PINDLY locus of Arabidopsisalter gibberellin signal transduction.Plant Cell, 5, 887–896.
Jacobsen, S.E., Binkowski, K.A. and Olszewski, N.E.(1996) SPINDLY, a tetratricopeptide repeat protein involved in gibberellin signal transduction in Arabidopsis. Proc. Natl Acad. Sci. USA, 93, 9292–9296.
Swain, S.M., Tseng, T.-S. and Olszewski, N.E. (2001) Altered expression ofSPINDLYaffects gibberellin response and plant development.Plant Physiol.126, 1174–1185.
Thornton, T.M., Swain, S.M. and Olszewski, N.E.(1999) Gibberellin signal transduction presents…the SPY who O-GlcNAc’d me.Trends Plant Sci.4, 424–428.

==Structured Information==
{{JaponicaGene|
GeneName = Os08g0559300|
Description = Similar to Gibberellin action negative regulator SPY|
Version = NM_001069036.1 GI:115477810 GeneID:4346315|
Length = 7732 bp|
Definition = Oryza sativa Japonica Group Os08g0559300, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 8|Chromosome 8]]|
AP = Chromosome 8:28079564..28087295|
CDS = 28080313..28080681,28081406..28081567,28081662..28081837,28081928..28082003,28082401..28082529 ,28082610..28082729,28082819..28082885,28082979..28083062,28083263..28083357 ,28083674..28083738,28083851..28084049,28084673..28084867,28085113..28085207 ,28085306..28085441,28085512..28085729,28085976..28086103,28086395..28086864 |
GCID = <gbrowseImage1>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcgcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaaatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttgggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtga</cdnaseq>|
AA = <aaseq>MGRPGMDSSEGRESNGVVPERNGGAVPAKQQLDGKDTLRYANIL RSRNKFAEALQLYNNVLEKDEANVEALIGKGICLQAQSLPMQAIECFNEAVRIDPGNA CALTYCGMIYKDEGHLVEAAEAYQKARNADPSYKPAAEFLAIVLTDLGTSLKLAGNTE EGIQKYCEALEVDSHYAPAYYNLGVVYSEMMQFDLALTCYEKAALERPLYAEAYCNMG VIYKNRGELEAAIACYERCLTISPNFEIAKNNMAIALTDLGTKVKIEGDINQGVAYYK KALFYNWHYADAMYNLGVAYGEMLNFEMAIVFYELALHFNPRCAEACNNLGVIYKDRD NLDKAVECYQMALSIKPNFSQSLNNLGVVYTVQGKMDAASSMIQKAIFANSTYAEAYN NLGVLYRDAGSITSAVQAYEKCLQIDPDSRNAGQNRLLALNYIDEGFDDKLYQAHREW GKRFLKLYPQYTSWDNPKVADRPLVIGYVSPDYFTHSVSYFIEAPLAHHDYSNYKVVV YSGVVKADAKTLRFKDKVLKKGGLWRDIYGIDEKKVASLVREDKVDILVELTGHTANN KLGTMACRPAPIQVTWIGYPNTTGLPTIDYRITDSLADPPDTTQKHVEELVRLPESFL CYSPSPEAGPVCPTPAILNGFITFGSFNNLAKITPKVLQVWAKILCAVPNSRLVVKCK PFCCDSIRQKFLSTLAELGLEPLRVDLLPLIHLNHDHMQAYSLMDISLDTFPYAGTTT TCESLYMGVPCVTMAGSVHAHNVGVSLLTKVGLGRLVAKSENEYVSLALDLAADVTAL QELRMSLRGLMAKSPVCDGENFTRGLESAYRNMWRRYCDGDAPALRRLDLLQEEPCSN NNKQDFDDNQVAKLADLKAQRVDAAVDGDKQSQLTAHAAVVGEVQQAPIMVNGVSSPV SSGKVEANGHISR</aaseq>|
DNA = <dnaseqindica>750..1118#1843..2004#2099..2274#2365..2440#2838..2966#3047..3166#3256..3322#3416..3499#3700..3794#4111..4175#4288..4486#5110..5304#5550..5644#5743..5878#5949..6166#6413..6540#6832..7301#aaagaagggaaaaaaaatacgaaaagcaaaaaggaataaacggagggggctaaacaccaaaattccccctctccttctccttcccctcctcccgaaaccctcgccgccgtcgctgtcgaaaccctcgctgctgctgctgccgcacggcgcggcggggtaccgggtccccctccctgcggcggcgcggaggggtaggtctcccccccctcgctgcggcgtggcggtggggggtgcggtgccggctgccgcgccatgatcttgcgccggtggactgcgcctatccaatgtgagctctcctcctacttccgccgcggcattagggttcttgcttcttgagctggaagagttctcttgcgttgcgaacgcctactgtttcctgcgaattcttagtgcttgttcttggattctttccgggggtgttcttaatctgttgtttgcaaaagttctttttttctttttttggctagggttttgaaatttgcaatcttggtgttcttttccctgagtttttactaaaaagctgcggcttttacgcaattcggtggtagtccaaagccgcgtcctttttttctagtctttacgcgcatttggctccaatttttctcctttttttagacgcgttttcttcacgctaatattcgcaactgaaatcttagacctaacgaaccattctaaaattgatgatcgttacattctttctgcagaaaaaaaaaagataaagatagaacaagtaggttactttccgtaccatggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggtatgcgtaagattcttcatgtggattccctgttcttactgttagcttattcgttattgtgaaatctgatagtttttctttttagtttgttgtcataaacactagaagtctcaaatgttgtatatgtggttctctgaaagcaaaatactcaaatgctgaagtaagtgcctcgcacagtcgcactccccaaaataccaggcaatgagacttgggacttaactgccaatttctgacttctgagggtactgcagacctatttttagcaccaagttatatcttgagtggtttgtgtccaagaactgtaactagaaaccaaaacaactagtttgataagttcttgatcaagcatactatgcaagagataaaactgtcgagataatcactcaagaattttccagcagaagattttttattatctccatgacatacttattagcaccgtaaaattttgtgcgtagctggtggatagaatgacatgtgaatgagctaaggctcagctagagctgcaaaaagagtaatacttttgccccttggagaatagtggtccctattaattggcagggttgtaaaaatcctaagttcctaaccaatctaacatataagatcaaaacagttggtcagtccaaaaattctctcggaaaagcttggaatttcgacattgtttctgtatttattatgtgatatatttatccataaatgttctgatggagagaaattgtaacaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcggtaccatcaccagctataatttatatctagatctctattattttgtttttgttatccatttaagttttttaatgtgttcatctgattaatgtagcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtaagctttattttttctgctcatacaaattatgctgattcccaaagtactgttgaaaactaatgttgacttatctttactgtatcccaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtgtggtcttgctaccttttgtttcaaccaaatcaacacattgcttcttgtggtgtcctgcatgtcaatcataatggttgccatcacttgcccatcatattagcagtcatgcgtgacaggcttgaactaagactagtgttgatttgaaaattgtctcaatcatgtgggttaatgaggaaacatcatgataactctatgttttttcgttgcttgtccgctcactttttactctgagcatagcaactctggcaatataattaattttgactaaccttagaatgaatcatatgaaatagacctttttaatttatactttggtatacctttaagaattgttaactctggcatttggtggtgatttttgtatttttctcttatcaattgacatttttcacaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggtcagtcataagaaaaaaatctttaatattctgtacataatgtcctctcttttgctgacactcttttctgttttccaaaggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaagtaagtggaactaattatgcagtatgcaatatctgcagatagtcacaagtatcacaacagattattcattatcttctattctgttgtagatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccaggcatgctcctctgctagtcttgcacaatttctattgggattggatgattgtatcatgttttagcttattgaatatgttgttcttcatatatagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtaatgtttaaccaaccaacatcatgagagcctcttaaattctgccatgtaatttgaaacagcattttccagctgagactcataacaactatataagcactaatcagtaattcatttcattgctttgcataattctctttttgatagctggaatgagactttaatacgtaactgacaatccatcactgctttcaccataggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcaggtactttctttgtcctaagcttgctaattaaaacttgtgtaatctttctgtgttgcatggagaattaaatttatttaagcaggtacagctaataattatacttatccatttagtagcaaactgggaagcatataaatgtttcttccttgtataaatagacaaatacttgtctattgcatgttcataatttgttccaaccttgatgttgcttgcccacggtatctgttttgctgctttttaaaaaaaaatatatatttgtggaatagctatggtttatatttgtaacattcaacttacgatatagcttgttctgcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacaggtcatttagcactttttcacaatatacagtcatatttatgttagattgttttttctgtttttatgtgttagaaattctcaactatatattcactgtattttatattttgcagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggtagatatagccgttccttttcttgtctctattttcctgcattttcacattgtgtcccaaaaaacttgtttagactgtcttgcctcattaagatttaaaatatatatggaaataatttatgcaaaaatattccttttgaaaacagaaatggtagttcaatggtcaataggatagtgaactgtaattatttttcaagataggcctctagactatgtccagtttcaggattaccactttattattgcctagactgaaatgttttaaatagtgggctaagacatttagtggttagccttctcaaacagctatagctatagcggctaaattgtacataagagcaaatatttagctagacccttctcaaacagctatagccggagatttaaaatgcatatcctgaaaccccccaagaaagattatcccagtaacagcgaatatataattggaaatcatccaaggtggctgacaactcggggccatttatttgtgacatatcagttattttccaacttgcatgtttcactacttgtacaaagaaattaggcccaatttatctgcaatgtgttcgagtacctgttttaatcacagttcattcatgttttcttcaattgaaaattatttaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggtacctttgtgcccatgattcataactgtcacgttacctaatttcagaactatttggacagaagtgatatcatcagatcattatgttctgaaaccagcttcttctgatcttgctaacatgattgcttccttttcactctagtcaatgaaactattcaagtgtctaataataaatgattatctgactattctactatgtgattagtgaactgacaggggcttcttttacttgaattgcctgcacaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagtaagacatctgcttttcttgcggtgatagctttctggcatgcacttttatacccacacatctagaacgtgacggtgacaattatgaattatctgcaggcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaaggtgacacataaaacttgagtaatcagttcatttttactggatcactatttattgatttttcttttggcagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataaggtcagttatttagatacttcctccgtttcatattataagactttctagcattgtccacattcatatagatgttgtgtctagatttattaacatctatgtgaatgtggacaatgctagaaagtcttataatatgaaacggagggagtacatcttttatcctatgtttaaacatgttatgtaaacgagtagatccattctactgcattgtatcttaaattgtctgaactttggttggttcctttgcagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttggtacgtgtctatttactctgtggtgtatgtagcaatagatcgaattgctgtacagttttattttcatgctatttgttgatcgacttgttttcttcttttgcatcgccatgtgaagactcggaaaaaaaatcactattgtgctgtctggttttgagttttgatacatataacttgatgattggagcaggaattctagctattgaatctgacctttctttacacagttgaatcaggtcgggtatatgttctccttgccgaattgttggttgacatttactgttattcttgcagggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtgaccaaggggagtcgagcatactccattgatgtgggaaacactgacccactgtagcttgagatgcaccattgctgtggtggacatttgttcttacagaattcgacgctttgcaggcctacactgggtgtctcatttgtctgacacccacaatggataagaaagaagagcacttctgtgccatccgacggtgatttctctggttacactttgacctttgtagcaaatgtaagattgatgggggatatgcttatagagtttgatttgtagaggtataccccctgtgtaggtttatgaacatgatggcttcagttgccatatgttagaagaaacagagaaagaggaggaagcccttgttgtaaatgcagaaaaaacagaactgaaaaactactagaatcaattgacggcggcatttcaacaggctgtgtcatgatg</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001069036.1 RefSeq:Os08g0559300]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 8]]
[[Category:Chromosome 8]]

Os08g0559300

2014-05-22T08:44:28Z

Haidongli: /* References */

Please input one-sentence summary here.

==Annotated Information==
===Function===
SPINDLY(SPY) encodes an O-linked N-acetylglucosamine transferase that is considered to be a negative regulator of gibberellin (GA) signaling through an unknown mechanism(Jacobsen and Olszewski,1993; Jacobsenet al., 1996;Swain et al., 2001).Shimada et al(2006) isolated a rice SPINDLY homolog (OsSPY) and produced knockdown transgenic plants in which OsSPY expression was reduced by introducing its antisense or RNAi construct;they find that in knockdown plants, the enhanced elongation of lower internodes was correlated with decreased levels of OsSPY expression, similar to the spindly phenotype of Arabidopsis spy mutants,The suppression of OsSPY function in a GA‐insensitive mutant, gid2, also caused an increase in the phosphorylation of a rice DELLA protein, SLR1, but did not change the amount of SLR1.suggest that OsSPY functions as a negative factor in GA signaling in rice; OsSPY in GA signaling is not via changes in the amount or stability of SLR1, but probably involves control of the suppressive function of SLR1;and OsSPY may play roles both in GA signaling and in the brassinosteroid pathway.

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Jacobsen, S.E. and Olszewski, N.E.(1993) Mutations at theS PINDLY locus of Arabidopsisalter gibberellin signal transduction.Plant Cell, 5, 887–896.
Jacobsen, S.E., Binkowski, K.A. and Olszewski, N.E.(1996) SPINDLY, a tetratricopeptide repeat protein involved in gibberellin signal transduction in Arabidopsis. Proc. Natl Acad. Sci. USA, 93, 9292–9296.
Swain, S.M., Tseng, T.-S. and Olszewski, N.E. (2001) Altered expression ofSPINDLYaffects gibberellin response and plant development.Plant Physiol.126, 1174–1185.
Thornton, T.M., Swain, S.M. and Olszewski, N.E.(1999) Gibberellin signal transduction presents…the SPY who O-GlcNAc’d me.Trends Plant Sci.4, 424–428.

==Structured Information==
{{JaponicaGene|
GeneName = Os08g0559300|
Description = Similar to Gibberellin action negative regulator SPY|
Version = NM_001069036.1 GI:115477810 GeneID:4346315|
Length = 7732 bp|
Definition = Oryza sativa Japonica Group Os08g0559300, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 8|Chromosome 8]]|
AP = Chromosome 8:28079564..28087295|
CDS = 28080313..28080681,28081406..28081567,28081662..28081837,28081928..28082003,28082401..28082529 ,28082610..28082729,28082819..28082885,28082979..28083062,28083263..28083357 ,28083674..28083738,28083851..28084049,28084673..28084867,28085113..28085207 ,28085306..28085441,28085512..28085729,28085976..28086103,28086395..28086864 |
GCID = <gbrowseImage1>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcgcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaaatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttgggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtga</cdnaseq>|
AA = <aaseq>MGRPGMDSSEGRESNGVVPERNGGAVPAKQQLDGKDTLRYANIL RSRNKFAEALQLYNNVLEKDEANVEALIGKGICLQAQSLPMQAIECFNEAVRIDPGNA CALTYCGMIYKDEGHLVEAAEAYQKARNADPSYKPAAEFLAIVLTDLGTSLKLAGNTE EGIQKYCEALEVDSHYAPAYYNLGVVYSEMMQFDLALTCYEKAALERPLYAEAYCNMG VIYKNRGELEAAIACYERCLTISPNFEIAKNNMAIALTDLGTKVKIEGDINQGVAYYK KALFYNWHYADAMYNLGVAYGEMLNFEMAIVFYELALHFNPRCAEACNNLGVIYKDRD NLDKAVECYQMALSIKPNFSQSLNNLGVVYTVQGKMDAASSMIQKAIFANSTYAEAYN NLGVLYRDAGSITSAVQAYEKCLQIDPDSRNAGQNRLLALNYIDEGFDDKLYQAHREW GKRFLKLYPQYTSWDNPKVADRPLVIGYVSPDYFTHSVSYFIEAPLAHHDYSNYKVVV YSGVVKADAKTLRFKDKVLKKGGLWRDIYGIDEKKVASLVREDKVDILVELTGHTANN KLGTMACRPAPIQVTWIGYPNTTGLPTIDYRITDSLADPPDTTQKHVEELVRLPESFL CYSPSPEAGPVCPTPAILNGFITFGSFNNLAKITPKVLQVWAKILCAVPNSRLVVKCK PFCCDSIRQKFLSTLAELGLEPLRVDLLPLIHLNHDHMQAYSLMDISLDTFPYAGTTT TCESLYMGVPCVTMAGSVHAHNVGVSLLTKVGLGRLVAKSENEYVSLALDLAADVTAL QELRMSLRGLMAKSPVCDGENFTRGLESAYRNMWRRYCDGDAPALRRLDLLQEEPCSN NNKQDFDDNQVAKLADLKAQRVDAAVDGDKQSQLTAHAAVVGEVQQAPIMVNGVSSPV SSGKVEANGHISR</aaseq>|
DNA = <dnaseqindica>750..1118#1843..2004#2099..2274#2365..2440#2838..2966#3047..3166#3256..3322#3416..3499#3700..3794#4111..4175#4288..4486#5110..5304#5550..5644#5743..5878#5949..6166#6413..6540#6832..7301#aaagaagggaaaaaaaatacgaaaagcaaaaaggaataaacggagggggctaaacaccaaaattccccctctccttctccttcccctcctcccgaaaccctcgccgccgtcgctgtcgaaaccctcgctgctgctgctgccgcacggcgcggcggggtaccgggtccccctccctgcggcggcgcggaggggtaggtctcccccccctcgctgcggcgtggcggtggggggtgcggtgccggctgccgcgccatgatcttgcgccggtggactgcgcctatccaatgtgagctctcctcctacttccgccgcggcattagggttcttgcttcttgagctggaagagttctcttgcgttgcgaacgcctactgtttcctgcgaattcttagtgcttgttcttggattctttccgggggtgttcttaatctgttgtttgcaaaagttctttttttctttttttggctagggttttgaaatttgcaatcttggtgttcttttccctgagtttttactaaaaagctgcggcttttacgcaattcggtggtagtccaaagccgcgtcctttttttctagtctttacgcgcatttggctccaatttttctcctttttttagacgcgttttcttcacgctaatattcgcaactgaaatcttagacctaacgaaccattctaaaattgatgatcgttacattctttctgcagaaaaaaaaaagataaagatagaacaagtaggttactttccgtaccatggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggtatgcgtaagattcttcatgtggattccctgttcttactgttagcttattcgttattgtgaaatctgatagtttttctttttagtttgttgtcataaacactagaagtctcaaatgttgtatatgtggttctctgaaagcaaaatactcaaatgctgaagtaagtgcctcgcacagtcgcactccccaaaataccaggcaatgagacttgggacttaactgccaatttctgacttctgagggtactgcagacctatttttagcaccaagttatatcttgagtggtttgtgtccaagaactgtaactagaaaccaaaacaactagtttgataagttcttgatcaagcatactatgcaagagataaaactgtcgagataatcactcaagaattttccagcagaagattttttattatctccatgacatacttattagcaccgtaaaattttgtgcgtagctggtggatagaatgacatgtgaatgagctaaggctcagctagagctgcaaaaagagtaatacttttgccccttggagaatagtggtccctattaattggcagggttgtaaaaatcctaagttcctaaccaatctaacatataagatcaaaacagttggtcagtccaaaaattctctcggaaaagcttggaatttcgacattgtttctgtatttattatgtgatatatttatccataaatgttctgatggagagaaattgtaacaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcggtaccatcaccagctataatttatatctagatctctattattttgtttttgttatccatttaagttttttaatgtgttcatctgattaatgtagcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtaagctttattttttctgctcatacaaattatgctgattcccaaagtactgttgaaaactaatgttgacttatctttactgtatcccaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtgtggtcttgctaccttttgtttcaaccaaatcaacacattgcttcttgtggtgtcctgcatgtcaatcataatggttgccatcacttgcccatcatattagcagtcatgcgtgacaggcttgaactaagactagtgttgatttgaaaattgtctcaatcatgtgggttaatgaggaaacatcatgataactctatgttttttcgttgcttgtccgctcactttttactctgagcatagcaactctggcaatataattaattttgactaaccttagaatgaatcatatgaaatagacctttttaatttatactttggtatacctttaagaattgttaactctggcatttggtggtgatttttgtatttttctcttatcaattgacatttttcacaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggtcagtcataagaaaaaaatctttaatattctgtacataatgtcctctcttttgctgacactcttttctgttttccaaaggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaagtaagtggaactaattatgcagtatgcaatatctgcagatagtcacaagtatcacaacagattattcattatcttctattctgttgtagatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccaggcatgctcctctgctagtcttgcacaatttctattgggattggatgattgtatcatgttttagcttattgaatatgttgttcttcatatatagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtaatgtttaaccaaccaacatcatgagagcctcttaaattctgccatgtaatttgaaacagcattttccagctgagactcataacaactatataagcactaatcagtaattcatttcattgctttgcataattctctttttgatagctggaatgagactttaatacgtaactgacaatccatcactgctttcaccataggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcaggtactttctttgtcctaagcttgctaattaaaacttgtgtaatctttctgtgttgcatggagaattaaatttatttaagcaggtacagctaataattatacttatccatttagtagcaaactgggaagcatataaatgtttcttccttgtataaatagacaaatacttgtctattgcatgttcataatttgttccaaccttgatgttgcttgcccacggtatctgttttgctgctttttaaaaaaaaatatatatttgtggaatagctatggtttatatttgtaacattcaacttacgatatagcttgttctgcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacaggtcatttagcactttttcacaatatacagtcatatttatgttagattgttttttctgtttttatgtgttagaaattctcaactatatattcactgtattttatattttgcagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggtagatatagccgttccttttcttgtctctattttcctgcattttcacattgtgtcccaaaaaacttgtttagactgtcttgcctcattaagatttaaaatatatatggaaataatttatgcaaaaatattccttttgaaaacagaaatggtagttcaatggtcaataggatagtgaactgtaattatttttcaagataggcctctagactatgtccagtttcaggattaccactttattattgcctagactgaaatgttttaaatagtgggctaagacatttagtggttagccttctcaaacagctatagctatagcggctaaattgtacataagagcaaatatttagctagacccttctcaaacagctatagccggagatttaaaatgcatatcctgaaaccccccaagaaagattatcccagtaacagcgaatatataattggaaatcatccaaggtggctgacaactcggggccatttatttgtgacatatcagttattttccaacttgcatgtttcactacttgtacaaagaaattaggcccaatttatctgcaatgtgttcgagtacctgttttaatcacagttcattcatgttttcttcaattgaaaattatttaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggtacctttgtgcccatgattcataactgtcacgttacctaatttcagaactatttggacagaagtgatatcatcagatcattatgttctgaaaccagcttcttctgatcttgctaacatgattgcttccttttcactctagtcaatgaaactattcaagtgtctaataataaatgattatctgactattctactatgtgattagtgaactgacaggggcttcttttacttgaattgcctgcacaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagtaagacatctgcttttcttgcggtgatagctttctggcatgcacttttatacccacacatctagaacgtgacggtgacaattatgaattatctgcaggcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaaggtgacacataaaacttgagtaatcagttcatttttactggatcactatttattgatttttcttttggcagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataaggtcagttatttagatacttcctccgtttcatattataagactttctagcattgtccacattcatatagatgttgtgtctagatttattaacatctatgtgaatgtggacaatgctagaaagtcttataatatgaaacggagggagtacatcttttatcctatgtttaaacatgttatgtaaacgagtagatccattctactgcattgtatcttaaattgtctgaactttggttggttcctttgcagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttggtacgtgtctatttactctgtggtgtatgtagcaatagatcgaattgctgtacagttttattttcatgctatttgttgatcgacttgttttcttcttttgcatcgccatgtgaagactcggaaaaaaaatcactattgtgctgtctggttttgagttttgatacatataacttgatgattggagcaggaattctagctattgaatctgacctttctttacacagttgaatcaggtcgggtatatgttctccttgccgaattgttggttgacatttactgttattcttgcagggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtgaccaaggggagtcgagcatactccattgatgtgggaaacactgacccactgtagcttgagatgcaccattgctgtggtggacatttgttcttacagaattcgacgctttgcaggcctacactgggtgtctcatttgtctgacacccacaatggataagaaagaagagcacttctgtgccatccgacggtgatttctctggttacactttgacctttgtagcaaatgtaagattgatgggggatatgcttatagagtttgatttgtagaggtataccccctgtgtaggtttatgaacatgatggcttcagttgccatatgttagaagaaacagagaaagaggaggaagcccttgttgtaaatgcagaaaaaacagaactgaaaaactactagaatcaattgacggcggcatttcaacaggctgtgtcatgatg</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001069036.1 RefSeq:Os08g0559300]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 8]]
[[Category:Chromosome 8]]

Os08g0559300

2014-05-22T08:43:33Z

Haidongli: /* References */

Please input one-sentence summary here.

==Annotated Information==
===Function===
SPINDLY(SPY) encodes an O-linked N-acetylglucosamine transferase that is considered to be a negative regulator of gibberellin (GA) signaling through an unknown mechanism(Jacobsen and Olszewski,1993; Jacobsenet al., 1996;Swain et al., 2001).Shimada et al(2006) isolated a rice SPINDLY homolog (OsSPY) and produced knockdown transgenic plants in which OsSPY expression was reduced by introducing its antisense or RNAi construct;they find that in knockdown plants, the enhanced elongation of lower internodes was correlated with decreased levels of OsSPY expression, similar to the spindly phenotype of Arabidopsis spy mutants,The suppression of OsSPY function in a GA‐insensitive mutant, gid2, also caused an increase in the phosphorylation of a rice DELLA protein, SLR1, but did not change the amount of SLR1.suggest that OsSPY functions as a negative factor in GA signaling in rice; OsSPY in GA signaling is not via changes in the amount or stability of SLR1, but probably involves control of the suppressive function of SLR1;and OsSPY may play roles both in GA signaling and in the brassinosteroid pathway.

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.
Jacobsen, S.E. and Olszewski, N.E.(1993) Mutations at theS PINDLY locus of Arabidopsisalter gibberellin signal transduction.Plant Cell, 5, 887–896.
Jacobsen, S.E., Binkowski, K.A. and Olszewski, N.E.(1996) SPINDLY, a tetratricopeptide repeat protein involved in gibberellin signal transduction in Arabidopsis. Proc. Natl Acad. Sci. USA, 93, 9292–9296.
Swain, S.M., Tseng, T.-S. and Olszewski, N.E. (2001) Altered expression ofSPINDLYaffects gibberellin response and plant development.Plant Physiol.126, 1174–1185.
Thornton, T.M., Swain, S.M. and Olszewski, N.E.(1999) Gibberellin signal transduction presents…the SPY who O-GlcNAc’d me.Trends Plant Sci.4, 424–428.

==Structured Information==
{{JaponicaGene|
GeneName = Os08g0559300|
Description = Similar to Gibberellin action negative regulator SPY|
Version = NM_001069036.1 GI:115477810 GeneID:4346315|
Length = 7732 bp|
Definition = Oryza sativa Japonica Group Os08g0559300, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 8|Chromosome 8]]|
AP = Chromosome 8:28079564..28087295|
CDS = 28080313..28080681,28081406..28081567,28081662..28081837,28081928..28082003,28082401..28082529 ,28082610..28082729,28082819..28082885,28082979..28083062,28083263..28083357 ,28083674..28083738,28083851..28084049,28084673..28084867,28085113..28085207 ,28085306..28085441,28085512..28085729,28085976..28086103,28086395..28086864 |
GCID = <gbrowseImage1>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcgcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaaatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttgggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtga</cdnaseq>|
AA = <aaseq>MGRPGMDSSEGRESNGVVPERNGGAVPAKQQLDGKDTLRYANIL RSRNKFAEALQLYNNVLEKDEANVEALIGKGICLQAQSLPMQAIECFNEAVRIDPGNA CALTYCGMIYKDEGHLVEAAEAYQKARNADPSYKPAAEFLAIVLTDLGTSLKLAGNTE EGIQKYCEALEVDSHYAPAYYNLGVVYSEMMQFDLALTCYEKAALERPLYAEAYCNMG VIYKNRGELEAAIACYERCLTISPNFEIAKNNMAIALTDLGTKVKIEGDINQGVAYYK KALFYNWHYADAMYNLGVAYGEMLNFEMAIVFYELALHFNPRCAEACNNLGVIYKDRD NLDKAVECYQMALSIKPNFSQSLNNLGVVYTVQGKMDAASSMIQKAIFANSTYAEAYN NLGVLYRDAGSITSAVQAYEKCLQIDPDSRNAGQNRLLALNYIDEGFDDKLYQAHREW GKRFLKLYPQYTSWDNPKVADRPLVIGYVSPDYFTHSVSYFIEAPLAHHDYSNYKVVV YSGVVKADAKTLRFKDKVLKKGGLWRDIYGIDEKKVASLVREDKVDILVELTGHTANN KLGTMACRPAPIQVTWIGYPNTTGLPTIDYRITDSLADPPDTTQKHVEELVRLPESFL CYSPSPEAGPVCPTPAILNGFITFGSFNNLAKITPKVLQVWAKILCAVPNSRLVVKCK PFCCDSIRQKFLSTLAELGLEPLRVDLLPLIHLNHDHMQAYSLMDISLDTFPYAGTTT TCESLYMGVPCVTMAGSVHAHNVGVSLLTKVGLGRLVAKSENEYVSLALDLAADVTAL QELRMSLRGLMAKSPVCDGENFTRGLESAYRNMWRRYCDGDAPALRRLDLLQEEPCSN NNKQDFDDNQVAKLADLKAQRVDAAVDGDKQSQLTAHAAVVGEVQQAPIMVNGVSSPV SSGKVEANGHISR</aaseq>|
DNA = <dnaseqindica>750..1118#1843..2004#2099..2274#2365..2440#2838..2966#3047..3166#3256..3322#3416..3499#3700..3794#4111..4175#4288..4486#5110..5304#5550..5644#5743..5878#5949..6166#6413..6540#6832..7301#aaagaagggaaaaaaaatacgaaaagcaaaaaggaataaacggagggggctaaacaccaaaattccccctctccttctccttcccctcctcccgaaaccctcgccgccgtcgctgtcgaaaccctcgctgctgctgctgccgcacggcgcggcggggtaccgggtccccctccctgcggcggcgcggaggggtaggtctcccccccctcgctgcggcgtggcggtggggggtgcggtgccggctgccgcgccatgatcttgcgccggtggactgcgcctatccaatgtgagctctcctcctacttccgccgcggcattagggttcttgcttcttgagctggaagagttctcttgcgttgcgaacgcctactgtttcctgcgaattcttagtgcttgttcttggattctttccgggggtgttcttaatctgttgtttgcaaaagttctttttttctttttttggctagggttttgaaatttgcaatcttggtgttcttttccctgagtttttactaaaaagctgcggcttttacgcaattcggtggtagtccaaagccgcgtcctttttttctagtctttacgcgcatttggctccaatttttctcctttttttagacgcgttttcttcacgctaatattcgcaactgaaatcttagacctaacgaaccattctaaaattgatgatcgttacattctttctgcagaaaaaaaaaagataaagatagaacaagtaggttactttccgtaccatggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggtatgcgtaagattcttcatgtggattccctgttcttactgttagcttattcgttattgtgaaatctgatagtttttctttttagtttgttgtcataaacactagaagtctcaaatgttgtatatgtggttctctgaaagcaaaatactcaaatgctgaagtaagtgcctcgcacagtcgcactccccaaaataccaggcaatgagacttgggacttaactgccaatttctgacttctgagggtactgcagacctatttttagcaccaagttatatcttgagtggtttgtgtccaagaactgtaactagaaaccaaaacaactagtttgataagttcttgatcaagcatactatgcaagagataaaactgtcgagataatcactcaagaattttccagcagaagattttttattatctccatgacatacttattagcaccgtaaaattttgtgcgtagctggtggatagaatgacatgtgaatgagctaaggctcagctagagctgcaaaaagagtaatacttttgccccttggagaatagtggtccctattaattggcagggttgtaaaaatcctaagttcctaaccaatctaacatataagatcaaaacagttggtcagtccaaaaattctctcggaaaagcttggaatttcgacattgtttctgtatttattatgtgatatatttatccataaatgttctgatggagagaaattgtaacaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcggtaccatcaccagctataatttatatctagatctctattattttgtttttgttatccatttaagttttttaatgtgttcatctgattaatgtagcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtaagctttattttttctgctcatacaaattatgctgattcccaaagtactgttgaaaactaatgttgacttatctttactgtatcccaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtgtggtcttgctaccttttgtttcaaccaaatcaacacattgcttcttgtggtgtcctgcatgtcaatcataatggttgccatcacttgcccatcatattagcagtcatgcgtgacaggcttgaactaagactagtgttgatttgaaaattgtctcaatcatgtgggttaatgaggaaacatcatgataactctatgttttttcgttgcttgtccgctcactttttactctgagcatagcaactctggcaatataattaattttgactaaccttagaatgaatcatatgaaatagacctttttaatttatactttggtatacctttaagaattgttaactctggcatttggtggtgatttttgtatttttctcttatcaattgacatttttcacaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggtcagtcataagaaaaaaatctttaatattctgtacataatgtcctctcttttgctgacactcttttctgttttccaaaggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaagtaagtggaactaattatgcagtatgcaatatctgcagatagtcacaagtatcacaacagattattcattatcttctattctgttgtagatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccaggcatgctcctctgctagtcttgcacaatttctattgggattggatgattgtatcatgttttagcttattgaatatgttgttcttcatatatagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtaatgtttaaccaaccaacatcatgagagcctcttaaattctgccatgtaatttgaaacagcattttccagctgagactcataacaactatataagcactaatcagtaattcatttcattgctttgcataattctctttttgatagctggaatgagactttaatacgtaactgacaatccatcactgctttcaccataggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcaggtactttctttgtcctaagcttgctaattaaaacttgtgtaatctttctgtgttgcatggagaattaaatttatttaagcaggtacagctaataattatacttatccatttagtagcaaactgggaagcatataaatgtttcttccttgtataaatagacaaatacttgtctattgcatgttcataatttgttccaaccttgatgttgcttgcccacggtatctgttttgctgctttttaaaaaaaaatatatatttgtggaatagctatggtttatatttgtaacattcaacttacgatatagcttgttctgcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacaggtcatttagcactttttcacaatatacagtcatatttatgttagattgttttttctgtttttatgtgttagaaattctcaactatatattcactgtattttatattttgcagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggtagatatagccgttccttttcttgtctctattttcctgcattttcacattgtgtcccaaaaaacttgtttagactgtcttgcctcattaagatttaaaatatatatggaaataatttatgcaaaaatattccttttgaaaacagaaatggtagttcaatggtcaataggatagtgaactgtaattatttttcaagataggcctctagactatgtccagtttcaggattaccactttattattgcctagactgaaatgttttaaatagtgggctaagacatttagtggttagccttctcaaacagctatagctatagcggctaaattgtacataagagcaaatatttagctagacccttctcaaacagctatagccggagatttaaaatgcatatcctgaaaccccccaagaaagattatcccagtaacagcgaatatataattggaaatcatccaaggtggctgacaactcggggccatttatttgtgacatatcagttattttccaacttgcatgtttcactacttgtacaaagaaattaggcccaatttatctgcaatgtgttcgagtacctgttttaatcacagttcattcatgttttcttcaattgaaaattatttaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggtacctttgtgcccatgattcataactgtcacgttacctaatttcagaactatttggacagaagtgatatcatcagatcattatgttctgaaaccagcttcttctgatcttgctaacatgattgcttccttttcactctagtcaatgaaactattcaagtgtctaataataaatgattatctgactattctactatgtgattagtgaactgacaggggcttcttttacttgaattgcctgcacaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagtaagacatctgcttttcttgcggtgatagctttctggcatgcacttttatacccacacatctagaacgtgacggtgacaattatgaattatctgcaggcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaaggtgacacataaaacttgagtaatcagttcatttttactggatcactatttattgatttttcttttggcagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataaggtcagttatttagatacttcctccgtttcatattataagactttctagcattgtccacattcatatagatgttgtgtctagatttattaacatctatgtgaatgtggacaatgctagaaagtcttataatatgaaacggagggagtacatcttttatcctatgtttaaacatgttatgtaaacgagtagatccattctactgcattgtatcttaaattgtctgaactttggttggttcctttgcagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttggtacgtgtctatttactctgtggtgtatgtagcaatagatcgaattgctgtacagttttattttcatgctatttgttgatcgacttgttttcttcttttgcatcgccatgtgaagactcggaaaaaaaatcactattgtgctgtctggttttgagttttgatacatataacttgatgattggagcaggaattctagctattgaatctgacctttctttacacagttgaatcaggtcgggtatatgttctccttgccgaattgttggttgacatttactgttattcttgcagggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtgaccaaggggagtcgagcatactccattgatgtgggaaacactgacccactgtagcttgagatgcaccattgctgtggtggacatttgttcttacagaattcgacgctttgcaggcctacactgggtgtctcatttgtctgacacccacaatggataagaaagaagagcacttctgtgccatccgacggtgatttctctggttacactttgacctttgtagcaaatgtaagattgatgggggatatgcttatagagtttgatttgtagaggtataccccctgtgtaggtttatgaacatgatggcttcagttgccatatgttagaagaaacagagaaagaggaggaagcccttgttgtaaatgcagaaaaaacagaactgaaaaactactagaatcaattgacggcggcatttcaacaggctgtgtcatgatg</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001069036.1 RefSeq:Os08g0559300]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 8]]
[[Category:Chromosome 8]]

Os08g0559300

2014-05-22T08:24:21Z

Haidongli: /* Function */

Please input one-sentence summary here.

==Annotated Information==
===Function===
SPINDLY(SPY) encodes an O-linked N-acetylglucosamine transferase that is considered to be a negative regulator of gibberellin (GA) signaling through an unknown mechanism(Jacobsen and Olszewski,1993; Jacobsenet al., 1996;Swain et al., 2001).Shimada et al(2006) isolated a rice SPINDLY homolog (OsSPY) and produced knockdown transgenic plants in which OsSPY expression was reduced by introducing its antisense or RNAi construct;they find that in knockdown plants, the enhanced elongation of lower internodes was correlated with decreased levels of OsSPY expression, similar to the spindly phenotype of Arabidopsis spy mutants,The suppression of OsSPY function in a GA‐insensitive mutant, gid2, also caused an increase in the phosphorylation of a rice DELLA protein, SLR1, but did not change the amount of SLR1.suggest that OsSPY functions as a negative factor in GA signaling in rice; OsSPY in GA signaling is not via changes in the amount or stability of SLR1, but probably involves control of the suppressive function of SLR1;and OsSPY may play roles both in GA signaling and in the brassinosteroid pathway.

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os08g0559300|
Description = Similar to Gibberellin action negative regulator SPY|
Version = NM_001069036.1 GI:115477810 GeneID:4346315|
Length = 7732 bp|
Definition = Oryza sativa Japonica Group Os08g0559300, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 8|Chromosome 8]]|
AP = Chromosome 8:28079564..28087295|
CDS = 28080313..28080681,28081406..28081567,28081662..28081837,28081928..28082003,28082401..28082529 ,28082610..28082729,28082819..28082885,28082979..28083062,28083263..28083357 ,28083674..28083738,28083851..28084049,28084673..28084867,28085113..28085207 ,28085306..28085441,28085512..28085729,28085976..28086103,28086395..28086864 |
GCID = <gbrowseImage1>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008401:28079564..28087295
source=RiceChromosome08
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcgcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaaatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttgggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtga</cdnaseq>|
AA = <aaseq>MGRPGMDSSEGRESNGVVPERNGGAVPAKQQLDGKDTLRYANIL RSRNKFAEALQLYNNVLEKDEANVEALIGKGICLQAQSLPMQAIECFNEAVRIDPGNA CALTYCGMIYKDEGHLVEAAEAYQKARNADPSYKPAAEFLAIVLTDLGTSLKLAGNTE EGIQKYCEALEVDSHYAPAYYNLGVVYSEMMQFDLALTCYEKAALERPLYAEAYCNMG VIYKNRGELEAAIACYERCLTISPNFEIAKNNMAIALTDLGTKVKIEGDINQGVAYYK KALFYNWHYADAMYNLGVAYGEMLNFEMAIVFYELALHFNPRCAEACNNLGVIYKDRD NLDKAVECYQMALSIKPNFSQSLNNLGVVYTVQGKMDAASSMIQKAIFANSTYAEAYN NLGVLYRDAGSITSAVQAYEKCLQIDPDSRNAGQNRLLALNYIDEGFDDKLYQAHREW GKRFLKLYPQYTSWDNPKVADRPLVIGYVSPDYFTHSVSYFIEAPLAHHDYSNYKVVV YSGVVKADAKTLRFKDKVLKKGGLWRDIYGIDEKKVASLVREDKVDILVELTGHTANN KLGTMACRPAPIQVTWIGYPNTTGLPTIDYRITDSLADPPDTTQKHVEELVRLPESFL CYSPSPEAGPVCPTPAILNGFITFGSFNNLAKITPKVLQVWAKILCAVPNSRLVVKCK PFCCDSIRQKFLSTLAELGLEPLRVDLLPLIHLNHDHMQAYSLMDISLDTFPYAGTTT TCESLYMGVPCVTMAGSVHAHNVGVSLLTKVGLGRLVAKSENEYVSLALDLAADVTAL QELRMSLRGLMAKSPVCDGENFTRGLESAYRNMWRRYCDGDAPALRRLDLLQEEPCSN NNKQDFDDNQVAKLADLKAQRVDAAVDGDKQSQLTAHAAVVGEVQQAPIMVNGVSSPV SSGKVEANGHISR</aaseq>|
DNA = <dnaseqindica>750..1118#1843..2004#2099..2274#2365..2440#2838..2966#3047..3166#3256..3322#3416..3499#3700..3794#4111..4175#4288..4486#5110..5304#5550..5644#5743..5878#5949..6166#6413..6540#6832..7301#aaagaagggaaaaaaaatacgaaaagcaaaaaggaataaacggagggggctaaacaccaaaattccccctctccttctccttcccctcctcccgaaaccctcgccgccgtcgctgtcgaaaccctcgctgctgctgctgccgcacggcgcggcggggtaccgggtccccctccctgcggcggcgcggaggggtaggtctcccccccctcgctgcggcgtggcggtggggggtgcggtgccggctgccgcgccatgatcttgcgccggtggactgcgcctatccaatgtgagctctcctcctacttccgccgcggcattagggttcttgcttcttgagctggaagagttctcttgcgttgcgaacgcctactgtttcctgcgaattcttagtgcttgttcttggattctttccgggggtgttcttaatctgttgtttgcaaaagttctttttttctttttttggctagggttttgaaatttgcaatcttggtgttcttttccctgagtttttactaaaaagctgcggcttttacgcaattcggtggtagtccaaagccgcgtcctttttttctagtctttacgcgcatttggctccaatttttctcctttttttagacgcgttttcttcacgctaatattcgcaactgaaatcttagacctaacgaaccattctaaaattgatgatcgttacattctttctgcagaaaaaaaaaagataaagatagaacaagtaggttactttccgtaccatggggcgaccggggatggactccagcgaagggagggagagcaatggcgttgttcctgaacgtaatggtggtgctgtccctgcaaaacagcagttggacgggaaggacaccttgcggtacgccaacatcctccgctcccgcaacaaatttgccgaagcactccagttgtacaataatgtccttgagaaggatgaagccaatgtggaggcccttattggcaaagggatttgcctccaggcacagagcctacctatgcaggccatcgagtgcttcaacgaggccgtcaggatcgacccagggaatgcatgtgctctcacttattgtgggatgatatacaaagatgagggtcacttagttgaagctgcagaggtatgcgtaagattcttcatgtggattccctgttcttactgttagcttattcgttattgtgaaatctgatagtttttctttttagtttgttgtcataaacactagaagtctcaaatgttgtatatgtggttctctgaaagcaaaatactcaaatgctgaagtaagtgcctcgcacagtcgcactccccaaaataccaggcaatgagacttgggacttaactgccaatttctgacttctgagggtactgcagacctatttttagcaccaagttatatcttgagtggtttgtgtccaagaactgtaactagaaaccaaaacaactagtttgataagttcttgatcaagcatactatgcaagagataaaactgtcgagataatcactcaagaattttccagcagaagattttttattatctccatgacatacttattagcaccgtaaaattttgtgcgtagctggtggatagaatgacatgtgaatgagctaaggctcagctagagctgcaaaaagagtaatacttttgccccttggagaatagtggtccctattaattggcagggttgtaaaaatcctaagttcctaaccaatctaacatataagatcaaaacagttggtcagtccaaaaattctctcggaaaagcttggaatttcgacattgtttctgtatttattatgtgatatatttatccataaatgttctgatggagagaaattgtaacaggcttatcaaaaagctcgaaatgcagatccttcctacaaacctgctgcagagtttcttgctattgtgttgaccgatcttggaactagcttgaagcttgcaggtaatacagaggaggggattcaaaaatattgtgaagctttggaagttgacagccactatgcggtaccatcaccagctataatttatatctagatctctattattttgtttttgttatccatttaagttttttaatgtgttcatctgattaatgtagcctgcttattacaaccttggtgtggtttactcggagatgatgcaatttgatttggctcttacttgttacgagaaagctgctttggagaggccattgtatgctgaagcttattgcaacatgggagttatttacaagaatcgtggagaattagaagcagcaattgcctgctacgagaggtaagctttattttttctgctcatacaaattatgctgattcccaaagtactgttgaaaactaatgttgacttatctttactgtatcccaggtgcttgactatttccccaaattttgagattgccaagaataacatggcaatagcactaaccgacttgggtacaaaggtgtggtcttgctaccttttgtttcaaccaaatcaacacattgcttcttgtggtgtcctgcatgtcaatcataatggttgccatcacttgcccatcatattagcagtcatgcgtgacaggcttgaactaagactagtgttgatttgaaaattgtctcaatcatgtgggttaatgaggaaacatcatgataactctatgttttttcgttgcttgtccgctcactttttactctgagcatagcaactctggcaatataattaattttgactaaccttagaatgaatcatatgaaatagacctttttaatttatactttggtatacctttaagaattgttaactctggcatttggtggtgatttttgtatttttctcttatcaattgacatttttcacaggtaaaaattgagggtgacattaatcaaggtgtggcatattacaagaaagctctgttctacaattggcactacgctgatgccatgtataatcttggtgtcgcatatggtgaaatgttgaattttgagatggtcagtcataagaaaaaaatctttaatattctgtacataatgtcctctcttttgctgacactcttttctgttttccaaaggctattgttttttacgagcttgctctgcacttcaatcctcgctgtgcggaggcatgcaacaacctgggagtaatatacaaggacagggataatcttgacaaagctgttgaatgttatcaagtaagtggaactaattatgcagtatgcaatatctgcagatagtcacaagtatcacaacagattattcattatcttctattctgttgtagatggccttgtcaattaagccaaatttctctcagtcattgaataaccttggagttgtctacactgtccaggcatgctcctctgctagtcttgcacaatttctattgggattggatgattgtatcatgttttagcttattgaatatgttgttcttcatatatagggaaagatggatgctgcttcaagcatgattcagaaggccatatttgcaaattccacgtatgctgaagcatataataacttgggtaatgtttaaccaaccaacatcatgagagcctcttaaattctgccatgtaatttgaaacagcattttccagctgagactcataacaactatataagcactaatcagtaattcatttcattgctttgcataattctctttttgatagctggaatgagactttaatacgtaactgacaatccatcactgctttcaccataggtgttctttacagagatgcaggaagtattacttcagccgtacaagcttatgagaaatgccttcaaattgatcctgattcacgaaatgctggtcaggtactttctttgtcctaagcttgctaattaaaacttgtgtaatctttctgtgttgcatggagaattaaatttatttaagcaggtacagctaataattatacttatccatttagtagcaaactgggaagcatataaatgtttcttccttgtataaatagacaaatacttgtctattgcatgttcataatttgttccaaccttgatgttgcttgcccacggtatctgttttgctgctttttaaaaaaaaatatatatttgtggaatagctatggtttatatttgtaacattcaacttacgatatagcttgttctgcagaatcgtttgcttgcgttgaattatattgatgagggctttgacgacaagctttatcaagctcacaggtcatttagcactttttcacaatatacagtcatatttatgttagattgttttttctgtttttatgtgttagaaattctcaactatatattcactgtattttatattttgcagggagtggggaaaacgcttcctgaaattgtatccacagtatactagttgggataacccaaaagtcgctgatcgtcctctggtcattggctatgtgtctcctgattactttactcactctgtgtcatacttcattgaagctccccttgcacaccatgactactcaaattacaaggtggttgtgtattctggtgttgtgaaggtagatatagccgttccttttcttgtctctattttcctgcattttcacattgtgtcccaaaaaacttgtttagactgtcttgcctcattaagatttaaaatatatatggaaataatttatgcaaaaatattccttttgaaaacagaaatggtagttcaatggtcaataggatagtgaactgtaattatttttcaagataggcctctagactatgtccagtttcaggattaccactttattattgcctagactgaaatgttttaaatagtgggctaagacatttagtggttagccttctcaaacagctatagctatagcggctaaattgtacataagagcaaatatttagctagacccttctcaaacagctatagccggagatttaaaatgcatatcctgaaaccccccaagaaagattatcccagtaacagcgaatatataattggaaatcatccaaggtggctgacaactcggggccatttatttgtgacatatcagttattttccaacttgcatgtttcactacttgtacaaagaaattaggcccaatttatctgcaatgtgttcgagtacctgttttaatcacagttcattcatgttttcttcaattgaaaattatttaggcagatgccaagacccttcgattcaaggataaggtattaaaaaagggtgggttgtggagagatatatatggtattgatgaaaagaaggttgctagcttggtaagagaggacaaagtggatatacttgtggaacttactggtcatacggcaaataacaagttaggaacaatggcatgcaggcctgctcctattcaggtacctttgtgcccatgattcataactgtcacgttacctaatttcagaactatttggacagaagtgatatcatcagatcattatgttctgaaaccagcttcttctgatcttgctaacatgattgcttccttttcactctagtcaatgaaactattcaagtgtctaataataaatgattatctgactattctactatgtgattagtgaactgacaggggcttcttttacttgaattgcctgcacaggttacatggattggctaccctaatacaacaggtctgccaacaattgactacagaataacagattcattggctgacccacctgatacaacccaaaagtaagacatctgcttttcttgcggtgatagctttctggcatgcacttttatacccacacatctagaacgtgacggtgacaattatgaattatctgcaggcatgttgaagagttggtgcgccttcctgaaagctttctttgttacagtccttccccagaagctgggccagtttgtcccactccagcaattttaaatggtttcatcacgtttgggagttttaataatctagcaaaggtgacacataaaacttgagtaatcagttcatttttactggatcactatttattgatttttcttttggcagattacaccaaaagtattgcaagtttgggccaaaattttgtgtgcagtccctaactcccgtcttgtggttaagtgtaagccattctgctgcgacagtattagacagaaattcctatcaacactagcagaattgggtttggagccgttacgagttgacttgctgccactcatccatctcaaccatgatcacatgcaagcatattccctaatggacataaggtcagttatttagatacttcctccgtttcatattataagactttctagcattgtccacattcatatagatgttgtgtctagatttattaacatctatgtgaatgtggacaatgctagaaagtcttataatatgaaacggagggagtacatcttttatcctatgtttaaacatgttatgtaaacgagtagatccattctactgcattgtatcttaaattgtctgaactttggttggttcctttgcagcctggatacgtttccatatgctggaactactactacatgtgaatctctatacatgggggttccatgtgttactatggctggctcagtccatgctcataatgttggtgttagtctactcactaaagttggtacgtgtctatttactctgtggtgtatgtagcaatagatcgaattgctgtacagttttattttcatgctatttgttgatcgacttgttttcttcttttgcatcgccatgtgaagactcggaaaaaaaatcactattgtgctgtctggttttgagttttgatacatataacttgatgattggagcaggaattctagctattgaatctgacctttctttacacagttgaatcaggtcgggtatatgttctccttgccgaattgttggttgacatttactgttattcttgcagggctggggaggctggttgcgaagtctgagaatgaatatgttagcttagcattggatttagcggcagatgttactgccttgcaagaactgagaatgagcctccgagggctgatggcgaaatcaccagtgtgtgatggagagaatttcacacgtggtctggaatctgcatacagaaacatgtggcgcagatactgtgatggggatgcacctgccctcaggcggttggatctgttacaggaagagccatgctccaataacaataagcaagattttgatgacaatcaggtcgcaaagctcgcagatctgaaagcacagagagtggatgccgcggtagatggagacaagcaatcccagttaacagcgcatgctgcagtagtaggggaagttcagcaggccccaataatggtgaatggtgtgagttcacctgtctcttctgggaaagttgaagcaaatgggcacataagccggtgaccaaggggagtcgagcatactccattgatgtgggaaacactgacccactgtagcttgagatgcaccattgctgtggtggacatttgttcttacagaattcgacgctttgcaggcctacactgggtgtctcatttgtctgacacccacaatggataagaaagaagagcacttctgtgccatccgacggtgatttctctggttacactttgacctttgtagcaaatgtaagattgatgggggatatgcttatagagtttgatttgtagaggtataccccctgtgtaggtttatgaacatgatggcttcagttgccatatgttagaagaaacagagaaagaggaggaagcccttgttgtaaatgcagaaaaaacagaactgaaaaactactagaatcaattgacggcggcatttcaacaggctgtgtcatgatg</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001069036.1 RefSeq:Os08g0559300]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 8]]
[[Category:Chromosome 8]]