http://192.168.164.12:81/ricewiki/api.php?action=feedcontributions&feedformat=atom&user=Huangxiuting 2026-08-28T17:41:36Z User contributions MediaWiki 1.30.0 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0749300&diff=183264 2014-06-10T04:47:55Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> [[File:OsHsfA4a Fig.01.png|right|thumb|200px|Plant Proteins with Homology to Ta HsfA4a]]<br /> [[File:OsHsfA4a Fig.02.png|right|thumb|200px|The Cd-Sensitive Phenotype of hsfA4a-1 Knockdown Rice Plants]]<br /> [[File:OsHsfA4a Fig.03.png|right|thumb|200px|Identification of Amino Acid Residues in the DBD of Ta HsfA4a That Are Critical for Cd Tolerance]]<br /> Os HsfA4a is a rice ortholog of Ta HsfA4a which plays a role as Cd tolerance gene of Wheat. Ta HsfA4a is the most similar to the class A4 Hsfs that confer Cd tolerance to a Cd hypersensitive yeast strain identified Heat shock transcription factor A4a(HsfA4a). Cd tolerance is enhanced in rice plants expressing Ta HsfA4a and decreased in rice plant with knocked-down expression of Os HsfA4a. The DNA binding domain (DBD) of HsfA4a is critical for Cd tolerance, and within the DBD, Ala-31 and Leu-42 are important for Cd tolerance. Os HsfA4a confers Cd tolerance in the root of rice by upregulating metallothionein(MT) gene expression in planta. HsfA4a is unique not only in the high level of tolerance that it confers, but also in its Cd specificity. Os HsfA4a greatly enhances Cd tolerance in rice, but does not significantly enhance their tolerance to the other heavy metals, namely, lead, zinc, cobalt, manganese, silver, mercury, and iron.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0749300|<br /> Description = Similar to Heat shock factor|<br /> Version = NM_001050782.1 GI:115439934 GeneID:4325556|<br /> Length = 2317 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0749300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:33126259..33128575|<br /> CDS = 33126439..33126651,33127234..33128343|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:33126259..33128575<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:33126259..33128575<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggaggggggcggcgggggcgggtcgctgccgccgttcctgagcaagacgtacgagatggtggacgacccgtcgacggacgcggtggtggggtggacgcccgccggcaccagcttcgtcgtcgccaaccagcccgagttctgccgggatctgctccccaagtacttcaagcacaacaacttctccagcttcgtgcgacagctcaacacctatggctttaggaaagtagatccagaacaatgggaatttgcaaatgaggatttcataaaaggacagcggcaccggttgaaaaatatacatagacgcaaacctatattcagccattcatcacacagtcagggtgctggaccattaacagataatgaaaggaaagactatgaggaagaaatcgagaggctcaaatccgataatgcagcactgtcctcagagcttcaaaataatacgttgaagaaacttaatatggagaaacgaatgcaggctttggaagagaagctatttgttgtggaagatcagcagaggagtctgatatcatacgtcagagagattgtaaaggcaccaggatttctttctagctttgtacagcaacaagatcatcacaggaagaagaggagactgccaataccaatctccttccatgaggatgcaaatactcaggagaaccagatcatgccttgtgacttgaccaactcaccagctcagacattttacagagaatcatttgacaagatggaatcatccttgaactcattagaaaatttccttcgagaagcaagtgaagaatttggtaatgatatttcatatgatgatggtgtcccaggcccttcttcaactgttgttctcacagagctccattcacctggtgaaagtgatccccgtgtttcatcacctccaacaaggatgcgtacttcgtcagctggtgcaggagattcgcactcctctcgtgatgtagcagagtctaccagctgtgcagaaagtcctcctattcctcaaatgcattctcgtgtagatacacgagcgaaggtttctgaaatagatgttaattcagaacctgctgttacagaaactggtccatcaagagatcaacctgctgaagagcctcctgctgtcacacctggtgcaaacgatggcttctggcagcagtttcttaccgagcagcctggatcatcagatgcacatcaggaggctcaatcagaacggcgagatggaggaaacaaggtggatgagatgaaatcaggagatcgacaacatctttggtggggcaaaaggaacgttgagcagataacagaaaaacttggacttctcacctcaacggagaaaacctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MEGGGGGGSLPPFLSKTYEMVDDPSTDAVVGWTPAGTSFVVANQ PEFCRDLLPKYFKHNNFSSFVRQLNTYGFRKVDPEQWEFANEDFIKGQRHRLKNIHRR KPIFSHSSHSQGAGPLTDNERKDYEEEIERLKSDNAALSSELQNNTLKKLNMEKRMQA LEEKLFVVEDQQRSLISYVREIVKAPGFLSSFVQQQDHHRKKRRLPIPISFHEDANTQ ENQIMPCDLTNSPAQTFYRESFDKMESSLNSLENFLREASEEFGNDISYDDGVPGPSS TVVLTELHSPGESDPRVSSPPTRMRTSSAGAGDSHSSRDVAESTSCAESPPIPQMHSR VDTRAKVSEIDVNSEPAVTETGPSRDQPAEEPPAVTPGANDGFWQQFLTEQPGSSDAH QEAQSERRDGGNKVDEMKSGDRQHLWWGKRNVEQITEKLGLLTSTEKT&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;181..393#976..2085#aatccgagagggatttttcctcgcaaccaaccaaaccaaccaaacccccaaccaagcgagcaagccgccttcgccgggggggcccccaccctgacggcaaccctagccctccgatcaccggcgctgagactgatcccgccgccgtaggaggaggaggaggaggtggtggtggtggtggtgatggaggggggcggcgggggcgggtcgctgccgccgttcctgagcaagacgtacgagatggtggacgacccgtcgacggacgcggtggtggggtggacgcccgccggcaccagcttcgtcgtcgccaaccagcccgagttctgccgggatctgctccccaagtacttcaagcacaacaacttctccagcttcgtgcgacagctcaacacctatgtaagggagctcgcctcttaacctcatgtgtgcctttctgtcttcctctgctctgccgctgatgcttgaattggggcttggtcgtaatgcgcgcccgccgggacctatgggctgggtaggaatgccggatttgttagaattgggcgagtcctttgctgtaattactcctgtttaggatttattactttcagcacaaaatattttataagttttcgttgattgctagtatagcatattctgttcattttaggattttaagacagtacttgtgaataaccaaggtcaactcttgttctaccaagaaaattcaagtagcttattgcaatgttattgtcttctccatccggttgaagttgtacaaaaatgtttgataatggtgagtgaggttgaaatagatgtgtgttcacatcaatgtcaaagctccaaactgttttagaattgaagcaagctatccggtacagttcattccttgtggtggcagtgcatagttttttttagccttgatcatgtatgattttttttccccttttggctgcttgagttatgctattttaatgaggatccccttttgttctcatttagggctttaggaaagtagatccagaacaatgggaatttgcaaatgaggatttcataaaaggacagcggcaccggttgaaaaatatacatagacgcaaacctatattcagccattcatcacacagtcagggtgctggaccattaacagataatgaaaggaaagactatgaggaagaaatcgagaggctcaaatccgataatgcagcactgtcctcagagcttcaaaataatacgttgaagaaacttaatatggagaaacgaatgcaggctttggaagagaagctatttgttgtggaagatcagcagaggagtctgatatcatacgtcagagagattgtaaaggcaccaggatttctttctagctttgtacagcaacaagatcatcacaggaagaagaggagactgccaataccaatctccttccatgaggatgcaaatactcaggagaaccagatcatgccttgtgacttgaccaactcaccagctcagacattttacagagaatcatttgacaagatggaatcatccttgaactcattagaaaatttccttcgagaagcaagtgaagaatttggtaatgatatttcatatgatgatggtgtcccaggcccttcttcaactgttgttctcacagagctccattcacctggtgaaagtgatccccgtgtttcatcacctccaacaaggatgcgtacttcgtcagctggtgcaggagattcgcactcctctcgtgatgtagcagagtctaccagctgtgcagaaagtcctcctattcctcaaatgcattctcgtgtagatacacgagcgaaggtttctgaaatagatgttaattcagaacctgctgttacagaaactggtccatcaagagatcaacctgctgaagagcctcctgctgtcacacctggtgcaaacgatggcttctggcagcagtttcttaccgagcagcctggatcatcagatgcacatcaggaggctcaatcagaacggcgagatggaggaaacaaggtggatgagatgaaatcaggagatcgacaacatctttggtggggcaaaaggaacgttgagcagataacagaaaaacttggacttctcacctcaacggagaaaacctgacataattttgtttttctttttgtagatgaaaaactattaattcaggaacgctgacattggtgccttcctcagtgagatatgttgactaacatctatttcgcagtgtaccgtattttttccatcatgtaccgtaatgtatcatagtgccatacattcatctttggtccattgacactttctgatatcattgtgcgtgatttctaaaatattctcaagtttggttaaatgttac&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050782.1 RefSeq:Os01g0749300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0749300&diff=183255 2014-06-10T04:42:05Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> [[File:OsHsfA4a Fig.01.png|right|thumb|200px|]]<br /> Os HsfA4a is a rice ortholog of Ta HsfA4a which plays a role as Cd tolerance gene of Wheat. Ta HsfA4a is the most similar to the class A4 Hsfs that confer Cd tolerance to a Cd hypersensitive yeast strain identified Heat shock transcription factor A4a(HsfA4a). Cd tolerance is enhanced in rice plants expressing Ta HsfA4a and decreased in rice plant with knocked-down expression of Os HsfA4a. The DNA binding domain (DBD) of HsfA4a is critical for Cd tolerance, and within the DBD, Ala-31 and Leu-42 are important for Cd tolerance. Os HsfA4a confers Cd tolerance in the root of rice by upregulating metallothionein(MT) gene expression in planta. HsfA4a is unique not only in the high level of tolerance that it confers, but also in its Cd specificity. Os HsfA4a greatly enhances Cd tolerance in rice, but does not significantly enhance their tolerance to the other heavy metals, namely, lead, zinc, cobalt, manganese, silver, mercury, and iron.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0749300|<br /> Description = Similar to Heat shock factor|<br /> Version = NM_001050782.1 GI:115439934 GeneID:4325556|<br /> Length = 2317 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0749300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:33126259..33128575|<br /> CDS = 33126439..33126651,33127234..33128343|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:33126259..33128575<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:33126259..33128575<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggaggggggcggcgggggcgggtcgctgccgccgttcctgagcaagacgtacgagatggtggacgacccgtcgacggacgcggtggtggggtggacgcccgccggcaccagcttcgtcgtcgccaaccagcccgagttctgccgggatctgctccccaagtacttcaagcacaacaacttctccagcttcgtgcgacagctcaacacctatggctttaggaaagtagatccagaacaatgggaatttgcaaatgaggatttcataaaaggacagcggcaccggttgaaaaatatacatagacgcaaacctatattcagccattcatcacacagtcagggtgctggaccattaacagataatgaaaggaaagactatgaggaagaaatcgagaggctcaaatccgataatgcagcactgtcctcagagcttcaaaataatacgttgaagaaacttaatatggagaaacgaatgcaggctttggaagagaagctatttgttgtggaagatcagcagaggagtctgatatcatacgtcagagagattgtaaaggcaccaggatttctttctagctttgtacagcaacaagatcatcacaggaagaagaggagactgccaataccaatctccttccatgaggatgcaaatactcaggagaaccagatcatgccttgtgacttgaccaactcaccagctcagacattttacagagaatcatttgacaagatggaatcatccttgaactcattagaaaatttccttcgagaagcaagtgaagaatttggtaatgatatttcatatgatgatggtgtcccaggcccttcttcaactgttgttctcacagagctccattcacctggtgaaagtgatccccgtgtttcatcacctccaacaaggatgcgtacttcgtcagctggtgcaggagattcgcactcctctcgtgatgtagcagagtctaccagctgtgcagaaagtcctcctattcctcaaatgcattctcgtgtagatacacgagcgaaggtttctgaaatagatgttaattcagaacctgctgttacagaaactggtccatcaagagatcaacctgctgaagagcctcctgctgtcacacctggtgcaaacgatggcttctggcagcagtttcttaccgagcagcctggatcatcagatgcacatcaggaggctcaatcagaacggcgagatggaggaaacaaggtggatgagatgaaatcaggagatcgacaacatctttggtggggcaaaaggaacgttgagcagataacagaaaaacttggacttctcacctcaacggagaaaacctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MEGGGGGGSLPPFLSKTYEMVDDPSTDAVVGWTPAGTSFVVANQ PEFCRDLLPKYFKHNNFSSFVRQLNTYGFRKVDPEQWEFANEDFIKGQRHRLKNIHRR KPIFSHSSHSQGAGPLTDNERKDYEEEIERLKSDNAALSSELQNNTLKKLNMEKRMQA LEEKLFVVEDQQRSLISYVREIVKAPGFLSSFVQQQDHHRKKRRLPIPISFHEDANTQ ENQIMPCDLTNSPAQTFYRESFDKMESSLNSLENFLREASEEFGNDISYDDGVPGPSS TVVLTELHSPGESDPRVSSPPTRMRTSSAGAGDSHSSRDVAESTSCAESPPIPQMHSR VDTRAKVSEIDVNSEPAVTETGPSRDQPAEEPPAVTPGANDGFWQQFLTEQPGSSDAH QEAQSERRDGGNKVDEMKSGDRQHLWWGKRNVEQITEKLGLLTSTEKT&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;181..393#976..2085#aatccgagagggatttttcctcgcaaccaaccaaaccaaccaaacccccaaccaagcgagcaagccgccttcgccgggggggcccccaccctgacggcaaccctagccctccgatcaccggcgctgagactgatcccgccgccgtaggaggaggaggaggaggtggtggtggtggtggtgatggaggggggcggcgggggcgggtcgctgccgccgttcctgagcaagacgtacgagatggtggacgacccgtcgacggacgcggtggtggggtggacgcccgccggcaccagcttcgtcgtcgccaaccagcccgagttctgccgggatctgctccccaagtacttcaagcacaacaacttctccagcttcgtgcgacagctcaacacctatgtaagggagctcgcctcttaacctcatgtgtgcctttctgtcttcctctgctctgccgctgatgcttgaattggggcttggtcgtaatgcgcgcccgccgggacctatgggctgggtaggaatgccggatttgttagaattgggcgagtcctttgctgtaattactcctgtttaggatttattactttcagcacaaaatattttataagttttcgttgattgctagtatagcatattctgttcattttaggattttaagacagtacttgtgaataaccaaggtcaactcttgttctaccaagaaaattcaagtagcttattgcaatgttattgtcttctccatccggttgaagttgtacaaaaatgtttgataatggtgagtgaggttgaaatagatgtgtgttcacatcaatgtcaaagctccaaactgttttagaattgaagcaagctatccggtacagttcattccttgtggtggcagtgcatagttttttttagccttgatcatgtatgattttttttccccttttggctgcttgagttatgctattttaatgaggatccccttttgttctcatttagggctttaggaaagtagatccagaacaatgggaatttgcaaatgaggatttcataaaaggacagcggcaccggttgaaaaatatacatagacgcaaacctatattcagccattcatcacacagtcagggtgctggaccattaacagataatgaaaggaaagactatgaggaagaaatcgagaggctcaaatccgataatgcagcactgtcctcagagcttcaaaataatacgttgaagaaacttaatatggagaaacgaatgcaggctttggaagagaagctatttgttgtggaagatcagcagaggagtctgatatcatacgtcagagagattgtaaaggcaccaggatttctttctagctttgtacagcaacaagatcatcacaggaagaagaggagactgccaataccaatctccttccatgaggatgcaaatactcaggagaaccagatcatgccttgtgacttgaccaactcaccagctcagacattttacagagaatcatttgacaagatggaatcatccttgaactcattagaaaatttccttcgagaagcaagtgaagaatttggtaatgatatttcatatgatgatggtgtcccaggcccttcttcaactgttgttctcacagagctccattcacctggtgaaagtgatccccgtgtttcatcacctccaacaaggatgcgtacttcgtcagctggtgcaggagattcgcactcctctcgtgatgtagcagagtctaccagctgtgcagaaagtcctcctattcctcaaatgcattctcgtgtagatacacgagcgaaggtttctgaaatagatgttaattcagaacctgctgttacagaaactggtccatcaagagatcaacctgctgaagagcctcctgctgtcacacctggtgcaaacgatggcttctggcagcagtttcttaccgagcagcctggatcatcagatgcacatcaggaggctcaatcagaacggcgagatggaggaaacaaggtggatgagatgaaatcaggagatcgacaacatctttggtggggcaaaaggaacgttgagcagataacagaaaaacttggacttctcacctcaacggagaaaacctgacataattttgtttttctttttgtagatgaaaaactattaattcaggaacgctgacattggtgccttcctcagtgagatatgttgactaacatctatttcgcagtgtaccgtattttttccatcatgtaccgtaatgtatcatagtgccatacattcatctttggtccattgacactttctgatatcattgtgcgtgatttctaaaatattctcaagtttggttaaatgttac&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050782.1 RefSeq:Os01g0749300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:OsHsfA4a_Fig.01.png&diff=183252 2014-06-10T04:40:59Z

<p>Huangxiuting: </p> <hr /> <div></div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0749300&diff=183251 2014-06-10T04:39:39Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> [[File:OsHsfA4a Fig.01.png|right|thumb|200px|]]<br /> <br /> <br /> Os HsfA4a is a rice ortholog of Ta HsfA4a which plays a role as Cd tolerance gene of Wheat. Ta HsfA4a is the most similar to the class A4 Hsfs that confer Cd tolerance to a Cd hypersensitive yeast strain identified Heat shock transcription factor A4a(HsfA4a). Cd tolerance is enhanced in rice plants expressing Ta HsfA4a and decreased in rice plant with knocked-down expression of Os HsfA4a. The DNA binding domain (DBD) of HsfA4a is critical for Cd tolerance, and within the DBD, Ala-31 and Leu-42 are important for Cd tolerance. Os HsfA4a confers Cd tolerance in the root of rice by upregulating metallothionein(MT) gene expression in planta. HsfA4a is unique not only in the high level of tolerance that it confers, but also in its Cd specificity. Os HsfA4a greatly enhances Cd tolerance in rice, but does not significantly enhance their tolerance to the other heavy metals, namely, lead, zinc, cobalt, manganese, silver, mercury, and iron.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0749300|<br /> Description = Similar to Heat shock factor|<br /> Version = NM_001050782.1 GI:115439934 GeneID:4325556|<br /> Length = 2317 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0749300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:33126259..33128575|<br /> CDS = 33126439..33126651,33127234..33128343|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:33126259..33128575<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:33126259..33128575<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggaggggggcggcgggggcgggtcgctgccgccgttcctgagcaagacgtacgagatggtggacgacccgtcgacggacgcggtggtggggtggacgcccgccggcaccagcttcgtcgtcgccaaccagcccgagttctgccgggatctgctccccaagtacttcaagcacaacaacttctccagcttcgtgcgacagctcaacacctatggctttaggaaagtagatccagaacaatgggaatttgcaaatgaggatttcataaaaggacagcggcaccggttgaaaaatatacatagacgcaaacctatattcagccattcatcacacagtcagggtgctggaccattaacagataatgaaaggaaagactatgaggaagaaatcgagaggctcaaatccgataatgcagcactgtcctcagagcttcaaaataatacgttgaagaaacttaatatggagaaacgaatgcaggctttggaagagaagctatttgttgtggaagatcagcagaggagtctgatatcatacgtcagagagattgtaaaggcaccaggatttctttctagctttgtacagcaacaagatcatcacaggaagaagaggagactgccaataccaatctccttccatgaggatgcaaatactcaggagaaccagatcatgccttgtgacttgaccaactcaccagctcagacattttacagagaatcatttgacaagatggaatcatccttgaactcattagaaaatttccttcgagaagcaagtgaagaatttggtaatgatatttcatatgatgatggtgtcccaggcccttcttcaactgttgttctcacagagctccattcacctggtgaaagtgatccccgtgtttcatcacctccaacaaggatgcgtacttcgtcagctggtgcaggagattcgcactcctctcgtgatgtagcagagtctaccagctgtgcagaaagtcctcctattcctcaaatgcattctcgtgtagatacacgagcgaaggtttctgaaatagatgttaattcagaacctgctgttacagaaactggtccatcaagagatcaacctgctgaagagcctcctgctgtcacacctggtgcaaacgatggcttctggcagcagtttcttaccgagcagcctggatcatcagatgcacatcaggaggctcaatcagaacggcgagatggaggaaacaaggtggatgagatgaaatcaggagatcgacaacatctttggtggggcaaaaggaacgttgagcagataacagaaaaacttggacttctcacctcaacggagaaaacctga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MEGGGGGGSLPPFLSKTYEMVDDPSTDAVVGWTPAGTSFVVANQ PEFCRDLLPKYFKHNNFSSFVRQLNTYGFRKVDPEQWEFANEDFIKGQRHRLKNIHRR KPIFSHSSHSQGAGPLTDNERKDYEEEIERLKSDNAALSSELQNNTLKKLNMEKRMQA LEEKLFVVEDQQRSLISYVREIVKAPGFLSSFVQQQDHHRKKRRLPIPISFHEDANTQ ENQIMPCDLTNSPAQTFYRESFDKMESSLNSLENFLREASEEFGNDISYDDGVPGPSS TVVLTELHSPGESDPRVSSPPTRMRTSSAGAGDSHSSRDVAESTSCAESPPIPQMHSR VDTRAKVSEIDVNSEPAVTETGPSRDQPAEEPPAVTPGANDGFWQQFLTEQPGSSDAH QEAQSERRDGGNKVDEMKSGDRQHLWWGKRNVEQITEKLGLLTSTEKT&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;181..393#976..2085#aatccgagagggatttttcctcgcaaccaaccaaaccaaccaaacccccaaccaagcgagcaagccgccttcgccgggggggcccccaccctgacggcaaccctagccctccgatcaccggcgctgagactgatcccgccgccgtaggaggaggaggaggaggtggtggtggtggtggtgatggaggggggcggcgggggcgggtcgctgccgccgttcctgagcaagacgtacgagatggtggacgacccgtcgacggacgcggtggtggggtggacgcccgccggcaccagcttcgtcgtcgccaaccagcccgagttctgccgggatctgctccccaagtacttcaagcacaacaacttctccagcttcgtgcgacagctcaacacctatgtaagggagctcgcctcttaacctcatgtgtgcctttctgtcttcctctgctctgccgctgatgcttgaattggggcttggtcgtaatgcgcgcccgccgggacctatgggctgggtaggaatgccggatttgttagaattgggcgagtcctttgctgtaattactcctgtttaggatttattactttcagcacaaaatattttataagttttcgttgattgctagtatagcatattctgttcattttaggattttaagacagtacttgtgaataaccaaggtcaactcttgttctaccaagaaaattcaagtagcttattgcaatgttattgtcttctccatccggttgaagttgtacaaaaatgtttgataatggtgagtgaggttgaaatagatgtgtgttcacatcaatgtcaaagctccaaactgttttagaattgaagcaagctatccggtacagttcattccttgtggtggcagtgcatagttttttttagccttgatcatgtatgattttttttccccttttggctgcttgagttatgctattttaatgaggatccccttttgttctcatttagggctttaggaaagtagatccagaacaatgggaatttgcaaatgaggatttcataaaaggacagcggcaccggttgaaaaatatacatagacgcaaacctatattcagccattcatcacacagtcagggtgctggaccattaacagataatgaaaggaaagactatgaggaagaaatcgagaggctcaaatccgataatgcagcactgtcctcagagcttcaaaataatacgttgaagaaacttaatatggagaaacgaatgcaggctttggaagagaagctatttgttgtggaagatcagcagaggagtctgatatcatacgtcagagagattgtaaaggcaccaggatttctttctagctttgtacagcaacaagatcatcacaggaagaagaggagactgccaataccaatctccttccatgaggatgcaaatactcaggagaaccagatcatgccttgtgacttgaccaactcaccagctcagacattttacagagaatcatttgacaagatggaatcatccttgaactcattagaaaatttccttcgagaagcaagtgaagaatttggtaatgatatttcatatgatgatggtgtcccaggcccttcttcaactgttgttctcacagagctccattcacctggtgaaagtgatccccgtgtttcatcacctccaacaaggatgcgtacttcgtcagctggtgcaggagattcgcactcctctcgtgatgtagcagagtctaccagctgtgcagaaagtcctcctattcctcaaatgcattctcgtgtagatacacgagcgaaggtttctgaaatagatgttaattcagaacctgctgttacagaaactggtccatcaagagatcaacctgctgaagagcctcctgctgtcacacctggtgcaaacgatggcttctggcagcagtttcttaccgagcagcctggatcatcagatgcacatcaggaggctcaatcagaacggcgagatggaggaaacaaggtggatgagatgaaatcaggagatcgacaacatctttggtggggcaaaaggaacgttgagcagataacagaaaaacttggacttctcacctcaacggagaaaacctgacataattttgtttttctttttgtagatgaaaaactattaattcaggaacgctgacattggtgccttcctcagtgagatatgttgactaacatctatttcgcagtgtaccgtattttttccatcatgtaccgtaatgtatcatagtgccatacattcatctttggtccattgacactttctgatatcattgtgcgtgatttctaaaatattctcaagtttggttaaatgttac&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050782.1 RefSeq:Os01g0749300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180209 2014-06-07T13:01:30Z

<p>Huangxiuting: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> [[File:OsGR2 Fig.01.png|right|thumb|200px|Fig. 1 Changes of GR activity in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.02.png|right|thumb|200px|Fig. 2 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.03.png|right|thumb|200px|Fig. 3 Changes in H2O2 production in rice roots treated with or without NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.04.png|right|thumb|200px|Fig. 4 Effect of NaCl and H2O2 on mRNA levels for OsGR in roots of rice seedlings [1].]]<br /> <br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> <br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2].<br /> <br /> Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].<br /> Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].<br /> <br /> ===Expression===<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506 [4].[[File:OsGR2 Fig.5.png|right|thumb|200px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> <br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> <br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180208 2014-06-07T13:01:10Z

<p>Huangxiuting: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> [[File:OsGR2 Fig.01.png|right|thumb|200px|Fig. 1 Changes of GR activity in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.02.png|right|thumb|200px|Fig. 2 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.03.png|right|thumb|200px|Fig. 3 Changes in H2O2 production in rice roots treated with or without NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.04.png|right|thumb|200px|Fig. 4 Effect of NaCl and H2O2 on mRNA levels for OsGR in roots of rice seedlings [1].]]<br /> <br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> <br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2].<br /> <br /> Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].<br /> Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].<br /> <br /> ===Expression===<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506 [4].[[File:OsGR2 Fig.5.png|right|thumb|200px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> <br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180207 2014-06-07T13:00:34Z

<p>Huangxiuting: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> [[File:OsGR2 Fig.01.png|right|thumb|200px|Fig. 1 Changes of GR activity in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.02.png|right|thumb|200px|Fig. 2 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.03.png|right|thumb|200px|Fig. 3 Changes in H2O2 production in rice roots treated with or without NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.04.png|right|thumb|200px|Fig. 4 Effect of NaCl and H2O2 on mRNA levels for OsGR in roots of rice seedlings [1].]]<br /> <br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> <br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2].<br /> <br /> Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].<br /> Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].<br /> <br /> ===Expression===<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506 [4].[[File:OsGR2 Fig.5.png|right|thumb|200px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180206 2014-06-07T13:00:06Z

<p>Huangxiuting: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> [[File:OsGR2 Fig.01.png|right|thumb|200px|Fig. 1 Changes of GR activity in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.02.png|right|thumb|200px|Fig. 2 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.03.png|right|thumb|200px|Fig. 3 Changes in H2O2 production in rice roots treated with or without NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.04.png|right|thumb|200px|Fig. 4 Effect of NaCl and H2O2 on mRNA levels for OsGR in roots of rice seedlings [1].]]<br /> <br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> <br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2].<br /> <br /> Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].<br /> Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].<br /> <br /> ===Expression===<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506 [4].[[File:OsGR2 Fig.5.png|right|thumb|200px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180204 2014-06-07T12:59:44Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> [[File:OsGR2 Fig.01.png|right|thumb|200px|Fig. 1 Changes of GR activity in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.02.png|right|thumb|200px|Fig. 2 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.03.png|right|thumb|200px|Fig. 3 Changes in H2O2 production in rice roots treated with or without NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.04.png|right|thumb|200px|Fig. 4 Effect of NaCl and H2O2 on mRNA levels for OsGR in roots of rice seedlings [1].]]<br /> <br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> <br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2].<br /> <br /> Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].<br /> Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506 [4].[[File:OsGR2 Fig.5.png|right|thumb|200px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180201 2014-06-07T12:58:41Z

<p>Huangxiuting: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> [[File:OsGR2 Fig.01.png|right|thumb|200px|Fig. 1 Changes of GR activity in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.02.png|right|thumb|200px|Fig. 2 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.03.png|right|thumb|200px|Fig. 3 Changes in H2O2 production in rice roots treated with or without NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.04.png|right|thumb|200px|Fig. 4 Effect of NaCl and H2O2 on mRNA levels for OsGR in roots of rice seedlings [1].]]<br /> <br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> <br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2].<br /> <br /> Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].<br /> Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506 [4].[[File:OsGR2 Fig.5.png|right|thumb|200px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180197 2014-06-07T12:57:35Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> [[File:OsGR2 Fig.01.png|right|thumb|200px|Fig. 1 Changes of GR activity in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.02.png|right|thumb|200px|Fig. 2 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.03.png|right|thumb|200px|Fig. 3 Changes in H2O2 production in rice roots treated with or without NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.04.png|right|thumb|200px|Fig. 4 Effect of NaCl and H2O2 on mRNA levels for OsGR in roots of rice seedlings [1].]]<br /> <br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> <br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2].<br /> <br /> Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].<br /> Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:OsGR2_Fig.04.png&diff=180194 2014-06-07T12:55:27Z

<p>Huangxiuting: Fig. 4 Effect of NaCl and H2O2 on mRNA levels for OsGR in roots of rice seedlings [1].</p> <hr /> <div>Fig. 4 Effect of NaCl and H2O2 on mRNA levels for OsGR in roots of rice seedlings [1].</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180191 2014-06-07T12:54:43Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> [[File:OsGR2 Fig.01.png|right|thumb|200px|Fig. 1 Changes of GR activity in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.02.png|right|thumb|200px|Fig. 2 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.03.png|right|thumb|200px|Fig. 3 Changes in H2O2 production in rice roots treated with or without NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.04.png|right|thumb|200px|Fig. 4 Effect of NaCl and H2O2 on mRNA levels for OsGR in roots of rice seedlings [1].]]<br /> <br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> <br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180189 2014-06-07T12:53:34Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> [[File:OsGR2 Fig.01.png|right|thumb|200px|Fig. 1 Changes of GR activity in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.02.png|right|thumb|200px|Fig. 2 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.03.png|right|thumb|200px|Fig. 3 Changes in H2O2 production in rice roots treated with or without NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.03.png|right|thumb|200px|Fig. 4 Effect of NaCl and H2O2 on mRNA levels for OsGR in roots of rice seedlings [1].]]<br /> <br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> <br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:OsGR2_Fig.03.png&diff=180185 2014-06-07T12:51:40Z

<p>Huangxiuting: Fig. 3 Changes in H2O2 production in rice roots treated with or without NaCl [1].</p> <hr /> <div>Fig. 3 Changes in H2O2 production in rice roots treated with or without NaCl [1].</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180183 2014-06-07T12:50:29Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> [[File:OsGR2 Fig.01.png|right|thumb|300px|Fig. 1 Changes of GR activity in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.02.png|right|thumb|300px|Fig. 2 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.03.png|right|thumb|300px|Fig. 3 Changes in H2O2 production in rice roots treated with or without NaCl [1].]]<br /> <br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> <br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:OsGR2_Fig.02.png&diff=180180 2014-06-07T12:48:34Z

<p>Huangxiuting: Fig. 2 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].</p> <hr /> <div>Fig. 2 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180178 2014-06-07T12:47:44Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> [[File:OsGR2 Fig.01.png|right|thumb|300px|Fig. 1 Changes of GR activity in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> [[File:OsGR2 Fig.02.png|right|thumb|250px|Fig. 2 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> <br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:OsGR2_Fig.01.png&diff=180168 2014-06-07T12:43:49Z

<p>Huangxiuting: [[File:OsGR2 Fig.3.png|right|thumb|300px|Fig. 1 Changes of GR activity in rice roots in the presence or absence of NaCl [1].]]</p> <hr /> <div>[[File:OsGR2 Fig.3.png|right|thumb|300px|Fig. 1 Changes of GR activity in rice roots in the presence<br /> or absence of NaCl [1].]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180167 2014-06-07T12:43:21Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> [[File:OsGR2 Fig.01.png|right|thumb|300px|Fig. 1 Changes of GR activity in rice roots in the presence or absence of NaCl [1].]]<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> <br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180163 2014-06-07T12:42:00Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> [[File:OsGR2 Fig.11.png|right|thumb|300px|Fig. 1 Changes of GR activity in rice roots in the presence or absence of NaCl [1].]]<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> <br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180159 2014-06-07T12:40:01Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> [[File:OsGR2 Fig.3.png|right|thumb|300px|Fig. 1 Changes of GR activity in rice roots in the presence or absence of NaCl [1].]]<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> <br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:OsGR2_Fig.3.png&diff=180156 2014-06-07T12:38:20Z

<p>Huangxiuting: Fig. 1 Changes of GR activity in rice roots in the presence or absence of NaCl [1].</p> <hr /> <div>Fig. 1 Changes of GR activity in rice roots in the presence or absence of NaCl [1].</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180155 2014-06-07T12:37:28Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.[[File:OsGR2 Fig.3.png|right|thumb|300px|Fig. 1 Changes of GR activity in rice roots in the presence<br /> or absence of NaCl [1].]]<br /> <br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180154 2014-06-07T12:36:36Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.[[File:OsGR2 Fig.1.png|right|thumb|300px|Fig. 1 Changes of GR activity in rice roots in the presence<br /> or absence of NaCl [1].]]<br /> <br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.2.png|center|thumb|250px]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180145 2014-06-07T12:31:25Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> <br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.2.png|center|thumb|250px]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180142 2014-06-07T12:30:27Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> <br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.1.png|right|thumb|250px]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180139 2014-06-07T12:27:39Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180138 2014-06-07T12:25:54Z

<p>Huangxiuting: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.1.png|center|thumb|250px|Fig. 1 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180137 2014-06-07T12:24:22Z

<p>Huangxiuting: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.1.png|center|thumb|250px|Fig. 1 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].[[File:OsGR2 Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180133 2014-06-07T12:22:33Z

<p>Huangxiuting: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.1.png|center|thumb|250px|Fig. 1 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].[[File:OsGR2 Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180130 2014-06-07T12:21:45Z

<p>Huangxiuting: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.1.png|center|thumb|250px|Fig. 1 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].[[File:OsGR2 Evolution Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180127 2014-06-07T12:19:50Z

<p>Huangxiuting: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.1.png|center|thumb|250px|Fig. 1 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].[[File:OsGR2 Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180123 2014-06-07T12:17:44Z

<p>Huangxiuting: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.1.png|center|thumb|250px|Fig. 1 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|center|thumb|250px]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].[[File:OsGR2 Fig.5.png|right|thumb|250px|Fig. 5 Multiple alignment of a rice glutathione reductase (GR) and the other plant GRs [4].]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180118 2014-06-07T12:12:15Z

<p>Huangxiuting: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.1.png|center|thumb|250px|Fig. 1 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|center|thumb|250px]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180117 2014-06-07T12:11:35Z

<p>Huangxiuting: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.1.png|center|thumb|250px|Fig. 1 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|center|thumb|250px]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> <br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> <br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> <br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180116 2014-06-07T12:10:46Z

<p>Huangxiuting: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.1.png|center|thumb|250px|Fig. 1 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|center|thumb|250px]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> <br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> <br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> <br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180114 2014-06-07T12:09:43Z

<p>Huangxiuting: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.1.png|center|thumb|250px|Fig. 1 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|center|thumb|250px]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.<br /> <br /> ==References==<br /> Please input cited references here.<br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180112 2014-06-07T12:08:18Z

<p>Huangxiuting: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.1.png|center|thumb|250px|Fig. 1 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|center|thumb|250px]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of Chin<br /> <br /> ==References==<br /> Please input cited references here.<br /> [1]Hong C, Chao Y, Yang M, et al. NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil. 2009, 320: 103-115.<br /> [2] Wu T, Lin W, Kao Y, et al. Identification and characterization of a novel chloroplast_mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology. 2013, 83: 379-390.<br /> [3]Chou T, Chao Y, Kao C H. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology. 2012, 169: 478-486.<br /> [4] Kaminaka H, Morita S, Nakajima M, et al. Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice (Oryza Sativa L.). Plant Cell Physiology. 1998, 39(12): 1269-1280.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180108 2014-06-07T12:04:00Z

<p>Huangxiuting: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.1.png|center|thumb|250px|Fig. 1 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|center|thumb|250px]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.<br /> Kyoto Prefectural Institute of Agricultural Biotechnology, Seika, Kyoto, 619-0244 Japan.<br /> Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.<br /> Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of Chin<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180102 2014-06-07T12:00:24Z

<p>Huangxiuting: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.1.png|center|thumb|250px|Fig. 1 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|center|thumb|250px]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> In higher plants, GR is encoded by a small gene family. Two genes were described in Arabidopsis thaliana, Pisum sativum, Vigna unguiculata and Nicotiana tabacum, whereas 3 genes were identified in the genomes of Oryza sativa and Populus trichocarpa[2].The deduced amino acid sequences of RGRC2 showed a high preservation of some binding sites and active residues by comparison with previously published GR sequences from other plants, animals and prokaryotes[4].<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:OsGR2_Fig.5.png&diff=180087 2014-06-07T11:48:47Z

<p>Huangxiuting: </p> <hr /> <div></div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180085 2014-06-07T11:48:01Z

<p>Huangxiuting: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.1.png|center|thumb|250px|Fig. 1 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The cDNA of OsGR2 was firstly isolated in 1998 and was named RGRC2. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues[4]. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. RGRC2 is 1,822 bp in length and has an open reading frame encoding a polypeptide of 496 amino acids with a predicted molecular weight of 53,506[4].[[File:OsGR2 Fig.5.png|center|thumb|250px]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180070 2014-06-07T11:10:19Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.1.png|center|thumb|250px|Fig. 1 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180069 2014-06-07T11:09:27Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.1.png|right|thumb|200px|Fig. 1 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:OsGR2_Fig.1.png&diff=180066 2014-06-07T11:04:25Z

<p>Huangxiuting: Fig. 1 Changes in mRNA levels of OsGR genes in rice roots in the presence or absence of NaCl [1].</p> <hr /> <div>Fig. 1 Changes in mRNA levels of OsGR genes in rice roots<br /> in the presence or absence of NaCl [1].</div>

Huangxiuting https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0813500&diff=180065 2014-06-07T11:03:04Z

<p>Huangxiuting: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> The gene Os02g0813500 encodes a glutathione reductase named OsGR2. Glutathione reductase (GR; EC 1.6.4.2) is a flavoprotein oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to GSH with the accompanying oxidation of NADPH. GR is thought to be an important component of the glutathione ascorbate cycle, the main mechanism for the detoxification of ROS in plant.<br /> In plants, GR is located in different cellular compartments. Three genes encoding GR have been described for Oryza sativa: a cytosolic (OsGR2) and two chloroplastic isoforms (OsGR1 and OsGR3).OsGR1, OsGR2 and OsGR3 are located on chromosomes 3, 2, and 10, respectively [1].The expression of OsGR genes is influenced by abiotic and biotic stresses known to increase ROS production: drought/desiccation, salinity, chilling, cadmium, powdery mildew and abscisic acid (ABA) treatment [2]. Changes in enzymatic activity of GR have been positively associated with salt stress tolerance in rice. NaCl treatment could induce H2O2 production in rice roots, and H2O2 treatment resulted in enhanced OsGR2 and OsGR3 induction. H2O2 is involved in regulation of OsGR2 and OsGR3 expression in NaCl treated rice roots [1].Both heat shock (HS) and cadmium( Cd) can increase H2O2 content. HS and Cd treatments increased activities of GR in rice leaves through increasing the content of H2O2. Cd did not increase the expression of OsAPX (ascorbate peroxidase) and OsGR without HS treatment [3].[[File:OsGR2 Fig.1.png]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0813500|<br /> Description = Glutathione reductase, cytosolic (EC 1.8.1.7) (GR) (GRase)|<br /> Version = NM_001055020.1 GI:115449516 GeneID:4331112|<br /> Length = 5983 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0813500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:35724714..35730696|<br /> CDS = 35725003..35725143,35725246..35725310,35725464..35725533,35725867..35725938,35726036..35726239&lt;br&gt;,35727161..35727234,35727333..35727416,35727950..35728037,35728131..35728212&lt;br&gt;,35728546..35728619,35728842..35728949,35729051..35729115,35729685..35729798&lt;br&gt;,35730012..35730111,35730208..35730261,35730340..35730435|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:35724714..35730696<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcggatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctccatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaaacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MARKMLKDEEVEVAVTDGGSYDYDLFVIGAGSGGVRGSRTSASF GAKVAICELPFHPISSDWQGGHGGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEIN GDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSK QRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASI WKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKV VTDKGEEIIADVVLFATGRTPNSQRLNLEAAGVEVDNIGAIKVDDYSRTSVPNIWAVG DVTNRINLTPVALMEATCFSKTVFGGQPTKPDYRDVPCAVFSIPPLSVVGLSEQQALE EAKSDVLVYTSSFNPMKNSISKRQEKTVMKLVVDSETDKVLGASMCGPDAPEIIQGMA VALKCGATKATFDSTVGIHPSAAEEFVTMRTLTRRVSPSSKPKTNL&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;290..430#533..597#751..820#1154..1225#1323..1526#2448..2521#2620..2703#3237..3324#3418..3499#3833..3906#4129..4236#4338..4402#4972..5085#5299..5398#5495..5548#5627..5722#agaaacagatccaaccaattcgctcgcttaagccgccggcaaatcaacccaaccccaaggtacggtgctcacttctctctcttctcctttcctttttttttggttgtttttagcggtggattttgggctccacgcgcgtcgcggggcggcaatcgcgtctcatcaagtgattcctcgcgctaaagttcatcggatttggattgcaagttgttgtcaatctcaggaggggctctgtttctctctgttgtgcgtgtgcgcgttagggtttttcgtgtggtgttgaggatccatggctaggaagatgctcaaggacgaggaggtggaggtggccgtcaccgacggcgggagctacgactacgacctgttcgtgatcggcgccgggagcggcggcgtccggggctctcgcacctccgcgtccttcggggctaaggtttgacttttctcctctcccccgctccccatttttgcgaggaaacgtgttggatttttattttttttcgtgtgatttgattcggtgtgtggcgcggttcaggttgcgatttgcgagctcccgttccatcccatcagctcggattggcaaggagggcatggtgggacgtaagcatctgccgattacttgtctgcgtttacatttctgtcgttgaatgcttgattatgatttaaaccaactctcctcatcccctgggtgagaatgtgagatgttgctgcatcctaaaacccgcatgctcattattgtacatgttgtgctaggtgtgtgatacgtggttgtgtgcctaaaaagatactggtgtatggttcatctttccgcggagaatttgaggtaactgatcttactaacttatggagccaactattacttgtttgaaaaacaagttggaagcattgtgtttaggttcaatgtgctgtggcttgctgaattatgttcctttactcaatttttgcaaaatttgctaagccttacagcctccatccttgatcaacagttcatcattctgcgtactgacttgaaatccaataacctatccatttgagagcttgttatcatctttttatacaaactatttcatttagcttgcttgcatctttgtgagtacatgtactgaagtgtcattctggggtgcttattggaattgctactgttgtatgattgcaggatgcaaagaattttgggtgggaaatcaatggggacattaacttcaactggaaaaggctgctggaaaataaggtcagagccgcaaccttggttgggagaacgttcctgcattattatttgctcgttataaatgcttacaacccaatattgtaaaaaccgttctctgcagactcaagaaattgttagactaaatggagtataccagaggattcttggcaattctggtgtgacaatgattgaaggggcaggcagtttggttgatgctcatacagttgaagtcacaaagccagatggttcaaagcaaagatatacagcaaagcacatattgatagcaactggtagccgagctcaacgtgtcaacattcctgggaaggtaacaaaattctctgccagcctttgcaaacttgtttcttatgtctggcaagtagttaatagcattgttacttgtttgacaacttctttatattccccaacttttgtgcctgaaacctttggattgacctcgtgatgctgatttccatgccatcttctgtatttgttgtgatactgttgcacatcaataattatttgtgatagccagtttgatatggctgaacttttagggttaatcccctttagatatttcttattacttgacccaaaatacaatactccctctattccataatataaggcacaactactttttaaagctgttccataatataaggcatacatgcatgcatgccattaactaacacatattctccactaaaatattattatttaaattctccactatcaagatctctaattttattgggtgcatgtattacatttattagattaatctaaactatgaggtgattgaaggtggttgtgccttatattttggaatggagggagtactaggctacctttatatactgaggtgcctctagataagggaaacggatacaaactattaacctgatactactaggactcttataacaaaactatgaaactctgataaggagcttattaggactttccaactgctgaagcacttgccaaatctgacttatctctattacgccgccgctgatatactataccataacgtccagccatgacacagttggtgtggatttgacattacaagacttcctttgagccagttcaagagggacaaagcattatctttaatccaacaaattatacttagccatatatatgtgctggttacatcactgattttcttttccctttagtttttttttaaatgtcctttggtgtattattttggctgacactgataagaacccgcttgcaggagttagctattacttcagatgaggccttaagtttggaggagctaccaaaacgtgctgtaatccttggtggcgggtatgtctacactctagggtgcatttatgtactatatattgttatgtcattccaactatctcagacttgcatttgttcatgtggtggctatattgcagatatattgctgttgaatttgcttctatatggaaagggatgggtgcgcacgtagacttgttttatcgaaaagagcttcctctaaggtatttattataatctgttatctgcactgtgacctccagatatttaaaattgtacaccaaaacaatttactgctcagtatcagaaaaaagaacatcaatgtagcttctgtagttgcagtttttatgtcataatgtggctagagttgatagcatggttttctagtctaactgaagcttttggtcttgttttcagggctcagtcatgtgccactttagttactgtcctattttgacaatcttaattttttgccaaccctggtggttaatactacttgatcttctcgttttgtacattgatgcaatttattttctgaacattttttttgcaatatcatcaacctacaatgaaaacatggatgatgaaactagttatcagataaaacgatgcgattgcgaagcctggaccatgctatctgaacaactgaataatgattatggagaaagaatgccaatgtcatttttattagccaaaatttcaagctattgattacggttctatccaccatgattaatggcgaattgactaattgcagaggtttcgatgatgagatgaggacggttgttgcaagtaaccttgagggaaggggaatcagattacatccagggacaaatctatctgaggtgagttaagctgacatcctaaagtgcattttgattcccatgtgttctgttttcacgataggttttgatctgtttcttttttatgtaaaccagttgagtaaaacagccgatggcataaaagttgtcactgacaaaggagaggagatcattgcagatgttgttctgtttgctacaggtatttaggatagttcaatgctgaaagttatgttctctgtcactttaataatttctttgaatacatctctcttgatgactgttctgtgctggatgaaaaaaaatcatgtatcttctgatatttcacatggtcaagcaccatatgcatgtttgaaattgatcaatgtcatcctcacaacatttttttttatctcttgttgttgggaattgagatgctcaatatgagacattctacttttcacactatatttggaatgtggcatttatcctgattttcttttctttgaaggaaactttaatattgttgctatgcctggtatttaattgcgtgcaggtcgcacaccaaactcccagaggttgaacttggaagctgctggtgttgaagttgataatattggagctataaaggttgattttcttgccttattaattgaacccttacctgttaggctgttagcttgctgaagacatatttgttcatctgaaatttacttctcgtatttccttggagaaaacttacgcgtgaacctatgtgagaccatcttaggcatttaattggccattatctttgtatgatattctttgagactacaagtaatttgtgttgtttattcaatgttctatttttaggttgatgattattctcgtacatcagtcccaaatatatgggctgtgggtgatgtaacgaaccggataaatttaacacctgttgcactgatggaggctacctgcttttctgtaagtgactacagtgactcataatccccttgtaaattgggtgtgttcaaatttctttgtttctgtgtaaccagcaactttactttaatctttaattgcagaaaactgtgtttggtggccagccaactaaacctgattacagagatgtaccttgtgctgttttctcgtaagccaataaaccttgattgttctctgttatctccagtttttgtttttaactgaaacaagtagcatctacaagatggccacatatatacttcacaactctttgcttttgacactttcggtgaggcacactgtggtttggttttcttgtctgcacttcgaatgtttatttgcttagatcttagcagtgaaagagagtagcatgacgtgatatagggtaacttttctattcttccaggtttgactgagcagcatgaaacttgtgtgcggttgttatgctggatgtttcttaattctaattgatgcctatgtagtttagggtacattaggaagtaagcaacaattaaattctacattaacaaaacatggactagaatctagatagaaaaaaaattagtcatttcaatagacaagatctaaggatccttcactagtgatttttgcttttgccagtgcttctaccaatctattattcagtgcatttatggctgctgctgctttctttagattatctgcttttccacaatcatcgtgtcactcataacatggtttctttttttgttgaattagcatcccaccactatcagtagtgggcttgagtgaacagcaggctttggaggaagccaagagcgatgttcttgtttacacttccagcttcaacccaatgaagaacagcatatccaagtaagtatcatgtttattgcaagatcatctgttacgtcatagttacacctgactcctgagtgctttatcacttgaaggttctgggtatttcattattttcacttgtcatggtctcatggaccataaacattctagtaatggagcctatcagtgtcgtcattctttatagttcctgttctggtgaagtttcctgactcggctcactttttttagacggcaggagaagaccgtcatgaaactggtggttgattcagagactgataaagtacttggtgcatcaatgtgtggaccagatgcaccagagattatccaggtaagcaaagtttgtgtgtttatttcacacacaaaaaaatcgtggcattttccagtcttcactagtattttgcacctgactttccaccaattgcagggtatggctgtagcgctgaagtgtggagccaccaaggcgacctttgacagcactgtacgtggacacaacaataaaccagttgttaatatcatttggcactcgagtttctaatattatccattggctttgcaggttggtattcacccgtctgctgctgaagagtttgtgacaatgcggaccttgaccaggcgcgtgagcccatcatccaagccaaagacaaacttgtaggcaagatgagtaattttggataaagaaacatatatacccgttttgatttatattttgtggcaaagtgtactctggtttgcatctggtaatttcacgttagggattctacctggaacggtaaaaaggagacaatgtatacttgattgaaataaggtttctgcatatcagcctgtacagagagtaaaacttctgcgtttttctgatccaaataagcgggcgggacatcattcgttctgcctgcagacaaactctctgaatatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055020.1 RefSeq:Os02g0813500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

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