RiceWiki - User contributions [en]

Os07g0592600

2014-06-03T15:10:06Z

Hylwayne:

Please input one-sentence summary here.

==Annotated Information==
===Function===

OsMGH3 (OsGH3-8) is a member of group II GH3 proteins whose closest Arabidopsis sequence homolog AtGH3.2 functions as a negative component in auxin signaling by regulating auxin level and activity<ref name="ref1" /><ref name="ref2" />
Auxin plays a central role in nearly all aspects of plant growth and development. The final molecular and cellular outcome of auxin signaling in a specific tissue/spatially localized domain depends very critically on the level of its biologically active pool. This is mostly regulated by a homeostasis between its biosynthesis and inactivation. The significance of auxin homeostasis is underscored by the finding that in Arabidopsis_99% of the IAA pool is maintained in the conjugated inactive form generated by various GH3 proteins, and only 1% of IAA occurs as the biologically active form<ref name="ref3" />. Thus, regulated expression of GH3 factors can contribute to auxin signaling and these genes are ideal targets for transcription regulators to execute their developmental functions.

===Expression===
The expression domain of OsMGH3 overlaps with that of OsMADS1 at early stages of floret development (figure 1). In florets at later stages of development with differentiating organs, its expression overlaps more with OsMADS6 <ref name="ref4" /><ref name="ref5" /><ref name="ref6" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1">
Takase, T., Nakazawa, M., Ishikawa, A., Kawashima, M., Ichikawa, T., Takahashi, N. et al. (2004) ydk1-D, an auxin-responsive GH3 mutant that is involved in hypocotyl and root elongation. Plant J. 37:471–483.</ref>

<ref name="ref2" />
Staswick, P.E., Serban, B., Rowe, M., Tiryaki, I., Maldonado, M.T., Maldonado, M.C. et al. (2005) Characterization of an Arabidopsis enzyme family that conjugates amino acids to indole-3-acetic acid. Plant Cell 17: 616–627.
<ref name="ref3" />
Woodward, C., Bemis, S.M., Hill, E.J., Sawa, S., Koshiba, T. and Torii, K.U. (2005) Interaction of auxin and ERECTA in elaborating Arabidopsis inflorescence architecture revealed by the activation tagging of a new member of the YUCCA family putative flavin monooxygenases. Plant Physiol. 139: 192–203.
<ref name="ref4" />
Prasad, K., Parameswaran, S. and Vijayraghavan, U. (2005) OsMADS1, a rice MADS-box factor, controls differentiation of specific cell types in the lemma and palea and is an early-acting regulator of inner floral organs. Plant J. 43: 915–928.
<ref name="ref5" />
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os07g0592600|
Description = Similar to Indole-3-acetic acid-amido synthetase GH3.3 (EC 6.3.2.-) (Auxin- responsive GH3-like protein 3) (AtGH3-3)|
Version = NM_001066698.1 GI:115473128 GeneID:4343785|
Length = 2357 bp|
Definition = Oryza sativa Japonica Group Os07g0592600, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 7|Chromosome 7]]|
AP = Chromosome 7:24809875..24812231|
CDS = 24810162..24811536,24811631..24812073|
GCID = <gbrowseImage1>
name=NC_008400:24809875..24812231
source=RiceChromosome07
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008400:24809875..24812231
source=RiceChromosome07
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggtgatgactgatgtgtcgaccaccgggacggcactccggaccccggcggcgggggcggtgaaggagggcgacgtcgagaagctccggttcatcgacgagatgaccaccaacgtcgacgccgtgcaggagcgcgtcctgggggagatcctcggccgcaacgccggcacggagtacctgaccaagtgcggcctcgacggcgccaccgaccgcgccgccttccgggccaaggttccggtggtgtcgtacgacgacctgcagccgtacatccagcgcattgcgaacggggaccgctcgccgatcctgtccacccaccccgtctccgagttcctcaccagctccggcacgtcggccggcgagcgcaagctgatgcccaccatcatggacgagctcgaccgccgtcagctgctctacagcctcctcatgccggtcatgaacttgtatgtgcctgggcttgacaaggggaaggggctctacttcctgttcgtcaagtcggagacgaagacgccgggaggcctgacggcgaggcccgtgctgacgagctactacaagagcgaccacttcaagaaccggccgtacgacccgtaccacaactacacgagcccgacggcggccatcctctgcgccgacgcgttccagagcatgtacgcgcagatggtgtgcggcctgtgccagcgcaacgacgtgctgcgcctcggcgccgtgttcgcctccggcctcctccgggccatccgcttcctccagctcaactgggagcagctcgccgacgacatcgagtccggcgagctcacccctcgcgtcaccgacccgtcggtgcgcgaggctgtcgccgccatcctcctcccggaccccgagctcgccaagctcatccgcgccgagtgctccaagggcgactgggccggcatcatcacccgcgtgtggccgaacaccaagtacctcgacgtcatcgtcaccggcgcgatggcgcagtacatcccgaccctggagttctacagcggcgggcttcccatggcgtgcaccatgtacgcgtcatccgagtgctacttcggcctcaacctccgccccatgtgcgacccctccgaggtgtcctacaccatcatgcctaacatgggctacttcgagttcctccccgtggacgagaccggcgcggcttcgggcgacgccacccagctcgtggacctcgcccgcgtggaggtggggcgcgagtacgagctggtgatcaccacctacgccgggctgaaccggtaccgcgtcggcgacgtcctccgcgtgacggggttccacaacgcggcgccgcagttcaggttcgtgcgccgcaagaacgtgctcctctccatcgagtccgacaagaccgacgaggccgagctgcagcgcgccgtggagcgcgcgtcggcgctgctccgcccgcacggcgcgtccgtggtggagtacaccagccaagcgtgcaccaagcgcatcccgggccactacgtcatctactgggagctcctgaccaagggcgccggcgccacggtggtggacgcggacacgctgggcaggtgctgcctcgagatggaggaggcgctgaacacggtgtacaggcagagccgcgtcgcggacggctcgattgggccgctcgagatccgggtcgtccgcccgggcacattcgaggagctcatggactacgccatctcccgcggcgcgtccatcaaccagtacaaggtgccacgctgcgtcacgttcccgcccatcgtcgagctgctcgactcgcgcgtcgtgtccagccacttcagcccggcgctcccacactggactccggcgcgacggtccgaatag</cdnaseq>|
AA = <aaseq>MAVMTDVSTTGTALRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQ ERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYDDLQPYIQRIANGDRSPI LSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLYVPGLDKGKGLY FLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILCADAFQSMYA QMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVTDPSVREA VAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIPTLEFYSG GLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGAASGDATQL VDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSIESD KTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKGAGATVVD ADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGASINQ YKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARRSE</aaseq>|
DNA = <dnaseqindica>696..2070#159..601#aggccacacaccaccaacacaaaacatttccttgtccctcgccttcctcgcctcgcctcggctctcacacgacgcctcttcctctcgctactactagctcaagctaagctagcaagagcattccttgggctttttttcttgttcggggttgagaggcaatggcggtgatgactgatgtgtcgaccaccgggacggcactccggaccccggcggcgggggcggtgaaggagggcgacgtcgagaagctccggttcatcgacgagatgaccaccaacgtcgacgccgtgcaggagcgcgtcctgggggagatcctcggccgcaacgccggcacggagtacctgaccaagtgcggcctcgacggcgccaccgaccgcgccgccttccgggccaaggttccggtggtgtcgtacgacgacctgcagccgtacatccagcgcattgcgaacggggaccgctcgccgatcctgtccacccaccccgtctccgagttcctcaccagctccggcacgtcggccggcgagcgcaagctgatgcccaccatcatggacgagctcgaccgccgtcagctgctctacagcctcctcatgccggtcatgaacttgtaagttgatactactcctatcattgtctcattctgatagcacacacacgtacaagaagcagatgatgattaatggccatgaatcattgcataggtatgtgcctgggcttgacaaggggaaggggctctacttcctgttcgtcaagtcggagacgaagacgccgggaggcctgacggcgaggcccgtgctgacgagctactacaagagcgaccacttcaagaaccggccgtacgacccgtaccacaactacacgagcccgacggcggccatcctctgcgccgacgcgttccagagcatgtacgcgcagatggtgtgcggcctgtgccagcgcaacgacgtgctgcgcctcggcgccgtgttcgcctccggcctcctccgggccatccgcttcctccagctcaactgggagcagctcgccgacgacatcgagtccggcgagctcacccctcgcgtcaccgacccgtcggtgcgcgaggctgtcgccgccatcctcctcccggaccccgagctcgccaagctcatccgcgccgagtgctccaagggcgactgggccggcatcatcacccgcgtgtggccgaacaccaagtacctcgacgtcatcgtcaccggcgcgatggcgcagtacatcccgaccctggagttctacagcggcgggcttcccatggcgtgcaccatgtacgcgtcatccgagtgctacttcggcctcaacctccgccccatgtgcgacccctccgaggtgtcctacaccatcatgcctaacatgggctacttcgagttcctccccgtggacgagaccggcgcggcttcgggcgacgccacccagctcgtggacctcgcccgcgtggaggtggggcgcgagtacgagctggtgatcaccacctacgccgggctgaaccggtaccgcgtcggcgacgtcctccgcgtgacggggttccacaacgcggcgccgcagttcaggttcgtgcgccgcaagaacgtgctcctctccatcgagtccgacaagaccgacgaggccgagctgcagcgcgccgtggagcgcgcgtcggcgctgctccgcccgcacggcgcgtccgtggtggagtacaccagccaagcgtgcaccaagcgcatcccgggccactacgtcatctactgggagctcctgaccaagggcgccggcgccacggtggtggacgcggacacgctgggcaggtgctgcctcgagatggaggaggcgctgaacacggtgtacaggcagagccgcgtcgcggacggctcgattgggccgctcgagatccgggtcgtccgcccgggcacattcgaggagctcatggactacgccatctcccgcggcgcgtccatcaaccagtacaaggtgccacgctgcgtcacgttcccgcccatcgtcgagctgctcgactcgcgcgtcgtgtccagccacttcagcccggcgctcccacactggactccggcgcgacggtccgaatagtcggtcaaatccccacctcgtcgttggaccgtgtccaagaatctgaagtagtagaagccggatgcctccacctaaaaacacttatctgttatttttggaattaattttgatggttgctgtcaactctgtcagttagtggcaagagggtacctctgatgtgagggagagaactgcagaacgaacggaagaaagtgttgatgtaagaggttaccactagtagtactgtttgtctgtatcaggaatgtgattataatttaacctgtcctccaaaaccgtacgagtcctgc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001066698.1 RefSeq:Os07g0592600]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 7]]
[[Category:Chromosome 7]]

Os07g0592600

2014-06-03T15:08:32Z

Hylwayne:

Please input one-sentence summary here.

==Annotated Information==
===Function===

OsMGH3 (OsGH3-8) is a member of group II GH3 proteins whose closest Arabidopsis sequence homolog AtGH3.2 functions as a negative component in auxin signaling by regulating auxin level and activity<ref name="ref1" /><ref name="ref2" />
Auxin plays a central role in nearly all aspects of plant growth and development. The final molecular and cellular outcome of auxin signaling in a specific tissue/spatially localized domain depends very critically on the level of its biologically active pool. This is mostly regulated by a homeostasis between its biosynthesis and inactivation. The significance of auxin homeostasis is underscored by the finding that in Arabidopsis_99% of the IAA pool is maintained in the conjugated inactive form generated by various GH3 proteins, and only 1% of IAA occurs as the biologically active form<ref name="ref3" />. Thus, regulated expression of GH3 factors can contribute to auxin signaling and these genes are ideal targets for transcription regulators to execute their developmental functions.

===Expression===
The expression domain of OsMGH3 overlaps with that of OsMADS1 at early stages of floret development (figure 1). In florets at later stages of development with differentiating organs, its expression overlaps more with OsMADS6 <ref name="ref4" /><ref name="ref5" /><ref name="ref6" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1" />
Takase, T., Nakazawa, M., Ishikawa, A., Kawashima, M., Ichikawa, T., Takahashi, N. et al. (2004) ydk1-D, an auxin-responsive GH3 mutant that is involved in hypocotyl and root elongation. Plant J. 37:471–483.
<ref name="ref2" />
Staswick, P.E., Serban, B., Rowe, M., Tiryaki, I., Maldonado, M.T., Maldonado, M.C. et al. (2005) Characterization of an Arabidopsis enzyme family that conjugates amino acids to indole-3-acetic acid. Plant Cell 17: 616–627.
<ref name="ref3" />
Woodward, C., Bemis, S.M., Hill, E.J., Sawa, S., Koshiba, T. and Torii, K.U. (2005) Interaction of auxin and ERECTA in elaborating Arabidopsis inflorescence architecture revealed by the activation tagging of a new member of the YUCCA family putative flavin monooxygenases. Plant Physiol. 139: 192–203.
<ref name="ref4" />
Prasad, K., Parameswaran, S. and Vijayraghavan, U. (2005) OsMADS1, a rice MADS-box factor, controls differentiation of specific cell types in the lemma and palea and is an early-acting regulator of inner floral organs. Plant J. 43: 915–928.
<ref name="ref5" />
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os07g0592600|
Description = Similar to Indole-3-acetic acid-amido synthetase GH3.3 (EC 6.3.2.-) (Auxin- responsive GH3-like protein 3) (AtGH3-3)|
Version = NM_001066698.1 GI:115473128 GeneID:4343785|
Length = 2357 bp|
Definition = Oryza sativa Japonica Group Os07g0592600, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 7|Chromosome 7]]|
AP = Chromosome 7:24809875..24812231|
CDS = 24810162..24811536,24811631..24812073|
GCID = <gbrowseImage1>
name=NC_008400:24809875..24812231
source=RiceChromosome07
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008400:24809875..24812231
source=RiceChromosome07
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggtgatgactgatgtgtcgaccaccgggacggcactccggaccccggcggcgggggcggtgaaggagggcgacgtcgagaagctccggttcatcgacgagatgaccaccaacgtcgacgccgtgcaggagcgcgtcctgggggagatcctcggccgcaacgccggcacggagtacctgaccaagtgcggcctcgacggcgccaccgaccgcgccgccttccgggccaaggttccggtggtgtcgtacgacgacctgcagccgtacatccagcgcattgcgaacggggaccgctcgccgatcctgtccacccaccccgtctccgagttcctcaccagctccggcacgtcggccggcgagcgcaagctgatgcccaccatcatggacgagctcgaccgccgtcagctgctctacagcctcctcatgccggtcatgaacttgtatgtgcctgggcttgacaaggggaaggggctctacttcctgttcgtcaagtcggagacgaagacgccgggaggcctgacggcgaggcccgtgctgacgagctactacaagagcgaccacttcaagaaccggccgtacgacccgtaccacaactacacgagcccgacggcggccatcctctgcgccgacgcgttccagagcatgtacgcgcagatggtgtgcggcctgtgccagcgcaacgacgtgctgcgcctcggcgccgtgttcgcctccggcctcctccgggccatccgcttcctccagctcaactgggagcagctcgccgacgacatcgagtccggcgagctcacccctcgcgtcaccgacccgtcggtgcgcgaggctgtcgccgccatcctcctcccggaccccgagctcgccaagctcatccgcgccgagtgctccaagggcgactgggccggcatcatcacccgcgtgtggccgaacaccaagtacctcgacgtcatcgtcaccggcgcgatggcgcagtacatcccgaccctggagttctacagcggcgggcttcccatggcgtgcaccatgtacgcgtcatccgagtgctacttcggcctcaacctccgccccatgtgcgacccctccgaggtgtcctacaccatcatgcctaacatgggctacttcgagttcctccccgtggacgagaccggcgcggcttcgggcgacgccacccagctcgtggacctcgcccgcgtggaggtggggcgcgagtacgagctggtgatcaccacctacgccgggctgaaccggtaccgcgtcggcgacgtcctccgcgtgacggggttccacaacgcggcgccgcagttcaggttcgtgcgccgcaagaacgtgctcctctccatcgagtccgacaagaccgacgaggccgagctgcagcgcgccgtggagcgcgcgtcggcgctgctccgcccgcacggcgcgtccgtggtggagtacaccagccaagcgtgcaccaagcgcatcccgggccactacgtcatctactgggagctcctgaccaagggcgccggcgccacggtggtggacgcggacacgctgggcaggtgctgcctcgagatggaggaggcgctgaacacggtgtacaggcagagccgcgtcgcggacggctcgattgggccgctcgagatccgggtcgtccgcccgggcacattcgaggagctcatggactacgccatctcccgcggcgcgtccatcaaccagtacaaggtgccacgctgcgtcacgttcccgcccatcgtcgagctgctcgactcgcgcgtcgtgtccagccacttcagcccggcgctcccacactggactccggcgcgacggtccgaatag</cdnaseq>|
AA = <aaseq>MAVMTDVSTTGTALRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQ ERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYDDLQPYIQRIANGDRSPI LSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLYVPGLDKGKGLY FLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILCADAFQSMYA QMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVTDPSVREA VAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIPTLEFYSG GLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGAASGDATQL VDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSIESD KTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKGAGATVVD ADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGASINQ YKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARRSE</aaseq>|
DNA = <dnaseqindica>696..2070#159..601#aggccacacaccaccaacacaaaacatttccttgtccctcgccttcctcgcctcgcctcggctctcacacgacgcctcttcctctcgctactactagctcaagctaagctagcaagagcattccttgggctttttttcttgttcggggttgagaggcaatggcggtgatgactgatgtgtcgaccaccgggacggcactccggaccccggcggcgggggcggtgaaggagggcgacgtcgagaagctccggttcatcgacgagatgaccaccaacgtcgacgccgtgcaggagcgcgtcctgggggagatcctcggccgcaacgccggcacggagtacctgaccaagtgcggcctcgacggcgccaccgaccgcgccgccttccgggccaaggttccggtggtgtcgtacgacgacctgcagccgtacatccagcgcattgcgaacggggaccgctcgccgatcctgtccacccaccccgtctccgagttcctcaccagctccggcacgtcggccggcgagcgcaagctgatgcccaccatcatggacgagctcgaccgccgtcagctgctctacagcctcctcatgccggtcatgaacttgtaagttgatactactcctatcattgtctcattctgatagcacacacacgtacaagaagcagatgatgattaatggccatgaatcattgcataggtatgtgcctgggcttgacaaggggaaggggctctacttcctgttcgtcaagtcggagacgaagacgccgggaggcctgacggcgaggcccgtgctgacgagctactacaagagcgaccacttcaagaaccggccgtacgacccgtaccacaactacacgagcccgacggcggccatcctctgcgccgacgcgttccagagcatgtacgcgcagatggtgtgcggcctgtgccagcgcaacgacgtgctgcgcctcggcgccgtgttcgcctccggcctcctccgggccatccgcttcctccagctcaactgggagcagctcgccgacgacatcgagtccggcgagctcacccctcgcgtcaccgacccgtcggtgcgcgaggctgtcgccgccatcctcctcccggaccccgagctcgccaagctcatccgcgccgagtgctccaagggcgactgggccggcatcatcacccgcgtgtggccgaacaccaagtacctcgacgtcatcgtcaccggcgcgatggcgcagtacatcccgaccctggagttctacagcggcgggcttcccatggcgtgcaccatgtacgcgtcatccgagtgctacttcggcctcaacctccgccccatgtgcgacccctccgaggtgtcctacaccatcatgcctaacatgggctacttcgagttcctccccgtggacgagaccggcgcggcttcgggcgacgccacccagctcgtggacctcgcccgcgtggaggtggggcgcgagtacgagctggtgatcaccacctacgccgggctgaaccggtaccgcgtcggcgacgtcctccgcgtgacggggttccacaacgcggcgccgcagttcaggttcgtgcgccgcaagaacgtgctcctctccatcgagtccgacaagaccgacgaggccgagctgcagcgcgccgtggagcgcgcgtcggcgctgctccgcccgcacggcgcgtccgtggtggagtacaccagccaagcgtgcaccaagcgcatcccgggccactacgtcatctactgggagctcctgaccaagggcgccggcgccacggtggtggacgcggacacgctgggcaggtgctgcctcgagatggaggaggcgctgaacacggtgtacaggcagagccgcgtcgcggacggctcgattgggccgctcgagatccgggtcgtccgcccgggcacattcgaggagctcatggactacgccatctcccgcggcgcgtccatcaaccagtacaaggtgccacgctgcgtcacgttcccgcccatcgtcgagctgctcgactcgcgcgtcgtgtccagccacttcagcccggcgctcccacactggactccggcgcgacggtccgaatagtcggtcaaatccccacctcgtcgttggaccgtgtccaagaatctgaagtagtagaagccggatgcctccacctaaaaacacttatctgttatttttggaattaattttgatggttgctgtcaactctgtcagttagtggcaagagggtacctctgatgtgagggagagaactgcagaacgaacggaagaaagtgttgatgtaagaggttaccactagtagtactgtttgtctgtatcaggaatgtgattataatttaacctgtcctccaaaaccgtacgagtcctgc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001066698.1 RefSeq:Os07g0592600]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 7]]
[[Category:Chromosome 7]]

Os07g0592600

2014-06-03T15:07:18Z

Hylwayne:

Please input one-sentence summary here.

==Annotated Information==
===Function===

OsMGH3 (OsGH3-8) is a member of group II GH3 proteins whose closest Arabidopsis sequence homolog AtGH3.2 functions as a negative component in auxin signaling by regulating auxin level and activity<ref name="ref1" /><ref name="ref2" />
Auxin plays a central role in nearly all aspects of plant growth and development. The final molecular and cellular outcome of auxin signaling in a specific tissue/spatially localized domain depends very critically on the level of its biologically active pool. This is mostly regulated by a homeostasis between its biosynthesis and inactivation. The significance of auxin homeostasis is underscored by the finding that in Arabidopsis_99% of the IAA pool is maintained in the conjugated inactive form generated by various GH3 proteins, and only 1% of IAA occurs as the biologically active form<ref name="ref3" />. Thus, regulated expression of GH3 factors can contribute to auxin signaling and these genes are ideal targets for transcription regulators to execute their developmental functions.

===Expression===
The expression domain of OsMGH3 overlaps with that of OsMADS1 at early stages of floret development (figure 1). In florets at later stages of development with differentiating organs, its expression overlaps more with OsMADS6 <ref name="ref4" /><ref name="ref5" /><ref name="ref6" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref2" />
Staswick, P.E., Serban, B., Rowe, M., Tiryaki, I., Maldonado, M.T., Maldonado, M.C. et al. (2005) Characterization of an Arabidopsis enzyme family that conjugates amino acids to indole-3-acetic acid. Plant Cell 17: 616–627.
<ref name="ref3" />
Woodward, C., Bemis, S.M., Hill, E.J., Sawa, S., Koshiba, T. and Torii, K.U. (2005) Interaction of auxin and ERECTA in elaborating Arabidopsis inflorescence architecture revealed by the activation tagging of a new member of the YUCCA family putative flavin monooxygenases. Plant Physiol. 139: 192–203.
<ref name="ref4" />
Prasad, K., Parameswaran, S. and Vijayraghavan, U. (2005) OsMADS1, a rice MADS-box factor, controls differentiation of specific cell types in the lemma and palea and is an early-acting regulator of inner floral organs. Plant J. 43: 915–928.
<ref name="ref5" />
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os07g0592600|
Description = Similar to Indole-3-acetic acid-amido synthetase GH3.3 (EC 6.3.2.-) (Auxin- responsive GH3-like protein 3) (AtGH3-3)|
Version = NM_001066698.1 GI:115473128 GeneID:4343785|
Length = 2357 bp|
Definition = Oryza sativa Japonica Group Os07g0592600, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 7|Chromosome 7]]|
AP = Chromosome 7:24809875..24812231|
CDS = 24810162..24811536,24811631..24812073|
GCID = <gbrowseImage1>
name=NC_008400:24809875..24812231
source=RiceChromosome07
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008400:24809875..24812231
source=RiceChromosome07
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggtgatgactgatgtgtcgaccaccgggacggcactccggaccccggcggcgggggcggtgaaggagggcgacgtcgagaagctccggttcatcgacgagatgaccaccaacgtcgacgccgtgcaggagcgcgtcctgggggagatcctcggccgcaacgccggcacggagtacctgaccaagtgcggcctcgacggcgccaccgaccgcgccgccttccgggccaaggttccggtggtgtcgtacgacgacctgcagccgtacatccagcgcattgcgaacggggaccgctcgccgatcctgtccacccaccccgtctccgagttcctcaccagctccggcacgtcggccggcgagcgcaagctgatgcccaccatcatggacgagctcgaccgccgtcagctgctctacagcctcctcatgccggtcatgaacttgtatgtgcctgggcttgacaaggggaaggggctctacttcctgttcgtcaagtcggagacgaagacgccgggaggcctgacggcgaggcccgtgctgacgagctactacaagagcgaccacttcaagaaccggccgtacgacccgtaccacaactacacgagcccgacggcggccatcctctgcgccgacgcgttccagagcatgtacgcgcagatggtgtgcggcctgtgccagcgcaacgacgtgctgcgcctcggcgccgtgttcgcctccggcctcctccgggccatccgcttcctccagctcaactgggagcagctcgccgacgacatcgagtccggcgagctcacccctcgcgtcaccgacccgtcggtgcgcgaggctgtcgccgccatcctcctcccggaccccgagctcgccaagctcatccgcgccgagtgctccaagggcgactgggccggcatcatcacccgcgtgtggccgaacaccaagtacctcgacgtcatcgtcaccggcgcgatggcgcagtacatcccgaccctggagttctacagcggcgggcttcccatggcgtgcaccatgtacgcgtcatccgagtgctacttcggcctcaacctccgccccatgtgcgacccctccgaggtgtcctacaccatcatgcctaacatgggctacttcgagttcctccccgtggacgagaccggcgcggcttcgggcgacgccacccagctcgtggacctcgcccgcgtggaggtggggcgcgagtacgagctggtgatcaccacctacgccgggctgaaccggtaccgcgtcggcgacgtcctccgcgtgacggggttccacaacgcggcgccgcagttcaggttcgtgcgccgcaagaacgtgctcctctccatcgagtccgacaagaccgacgaggccgagctgcagcgcgccgtggagcgcgcgtcggcgctgctccgcccgcacggcgcgtccgtggtggagtacaccagccaagcgtgcaccaagcgcatcccgggccactacgtcatctactgggagctcctgaccaagggcgccggcgccacggtggtggacgcggacacgctgggcaggtgctgcctcgagatggaggaggcgctgaacacggtgtacaggcagagccgcgtcgcggacggctcgattgggccgctcgagatccgggtcgtccgcccgggcacattcgaggagctcatggactacgccatctcccgcggcgcgtccatcaaccagtacaaggtgccacgctgcgtcacgttcccgcccatcgtcgagctgctcgactcgcgcgtcgtgtccagccacttcagcccggcgctcccacactggactccggcgcgacggtccgaatag</cdnaseq>|
AA = <aaseq>MAVMTDVSTTGTALRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQ ERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYDDLQPYIQRIANGDRSPI LSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLYVPGLDKGKGLY FLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILCADAFQSMYA QMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVTDPSVREA VAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIPTLEFYSG GLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGAASGDATQL VDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSIESD KTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKGAGATVVD ADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGASINQ YKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARRSE</aaseq>|
DNA = <dnaseqindica>696..2070#159..601#aggccacacaccaccaacacaaaacatttccttgtccctcgccttcctcgcctcgcctcggctctcacacgacgcctcttcctctcgctactactagctcaagctaagctagcaagagcattccttgggctttttttcttgttcggggttgagaggcaatggcggtgatgactgatgtgtcgaccaccgggacggcactccggaccccggcggcgggggcggtgaaggagggcgacgtcgagaagctccggttcatcgacgagatgaccaccaacgtcgacgccgtgcaggagcgcgtcctgggggagatcctcggccgcaacgccggcacggagtacctgaccaagtgcggcctcgacggcgccaccgaccgcgccgccttccgggccaaggttccggtggtgtcgtacgacgacctgcagccgtacatccagcgcattgcgaacggggaccgctcgccgatcctgtccacccaccccgtctccgagttcctcaccagctccggcacgtcggccggcgagcgcaagctgatgcccaccatcatggacgagctcgaccgccgtcagctgctctacagcctcctcatgccggtcatgaacttgtaagttgatactactcctatcattgtctcattctgatagcacacacacgtacaagaagcagatgatgattaatggccatgaatcattgcataggtatgtgcctgggcttgacaaggggaaggggctctacttcctgttcgtcaagtcggagacgaagacgccgggaggcctgacggcgaggcccgtgctgacgagctactacaagagcgaccacttcaagaaccggccgtacgacccgtaccacaactacacgagcccgacggcggccatcctctgcgccgacgcgttccagagcatgtacgcgcagatggtgtgcggcctgtgccagcgcaacgacgtgctgcgcctcggcgccgtgttcgcctccggcctcctccgggccatccgcttcctccagctcaactgggagcagctcgccgacgacatcgagtccggcgagctcacccctcgcgtcaccgacccgtcggtgcgcgaggctgtcgccgccatcctcctcccggaccccgagctcgccaagctcatccgcgccgagtgctccaagggcgactgggccggcatcatcacccgcgtgtggccgaacaccaagtacctcgacgtcatcgtcaccggcgcgatggcgcagtacatcccgaccctggagttctacagcggcgggcttcccatggcgtgcaccatgtacgcgtcatccgagtgctacttcggcctcaacctccgccccatgtgcgacccctccgaggtgtcctacaccatcatgcctaacatgggctacttcgagttcctccccgtggacgagaccggcgcggcttcgggcgacgccacccagctcgtggacctcgcccgcgtggaggtggggcgcgagtacgagctggtgatcaccacctacgccgggctgaaccggtaccgcgtcggcgacgtcctccgcgtgacggggttccacaacgcggcgccgcagttcaggttcgtgcgccgcaagaacgtgctcctctccatcgagtccgacaagaccgacgaggccgagctgcagcgcgccgtggagcgcgcgtcggcgctgctccgcccgcacggcgcgtccgtggtggagtacaccagccaagcgtgcaccaagcgcatcccgggccactacgtcatctactgggagctcctgaccaagggcgccggcgccacggtggtggacgcggacacgctgggcaggtgctgcctcgagatggaggaggcgctgaacacggtgtacaggcagagccgcgtcgcggacggctcgattgggccgctcgagatccgggtcgtccgcccgggcacattcgaggagctcatggactacgccatctcccgcggcgcgtccatcaaccagtacaaggtgccacgctgcgtcacgttcccgcccatcgtcgagctgctcgactcgcgcgtcgtgtccagccacttcagcccggcgctcccacactggactccggcgcgacggtccgaatagtcggtcaaatccccacctcgtcgttggaccgtgtccaagaatctgaagtagtagaagccggatgcctccacctaaaaacacttatctgttatttttggaattaattttgatggttgctgtcaactctgtcagttagtggcaagagggtacctctgatgtgagggagagaactgcagaacgaacggaagaaagtgttgatgtaagaggttaccactagtagtactgtttgtctgtatcaggaatgtgattataatttaacctgtcctccaaaaccgtacgagtcctgc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001066698.1 RefSeq:Os07g0592600]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 7]]
[[Category:Chromosome 7]]

Os07g0592600

2014-06-03T15:03:59Z

Hylwayne:

Please input one-sentence summary here.

==Annotated Information==
===Function===

OsMGH3 (OsGH3-8) is a member of group II GH3 proteins whose closest Arabidopsis sequence homolog AtGH3.2 functions as a negative component in auxin signaling by regulating auxin level and activity<ref name="ref1" /><ref name="ref2" />
Auxin plays a central role in nearly all aspects of plant growth and development. The final molecular and cellular outcome of auxin signaling in a specific tissue/spatially localized domain depends very critically on the level of its biologically active pool. This is mostly regulated by a homeostasis between its biosynthesis and inactivation. The significance of auxin homeostasis is underscored by the finding that in Arabidopsis_99% of the IAA pool is maintained in the conjugated inactive form generated by various GH3 proteins, and only 1% of IAA occurs as the biologically active form<ref name="ref3" />. Thus, regulated expression of GH3 factors can contribute to auxin signaling and these genes are ideal targets for transcription regulators to execute their developmental functions.

===Expression===
The expression domain of OsMGH3 overlaps with that of OsMADS1 at early stages of floret development (figure 1). In florets at later stages of development with differentiating organs, its expression overlaps more with OsMADS6 <ref name="ref4" /><ref name="ref5" /><ref name="ref6" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==

<ref name="ref1" />
Takase, T., Nakazawa, M., Ishikawa, A., Kawashima, M., Ichikawa, T., Takahashi, N. et al. (2004) ydk1-D, an auxin-responsive GH3 mutant that is involved in hypocotyl and root elongation. Plant J. 37:471–483.

==Structured Information==
{{JaponicaGene|
GeneName = Os07g0592600|
Description = Similar to Indole-3-acetic acid-amido synthetase GH3.3 (EC 6.3.2.-) (Auxin- responsive GH3-like protein 3) (AtGH3-3)|
Version = NM_001066698.1 GI:115473128 GeneID:4343785|
Length = 2357 bp|
Definition = Oryza sativa Japonica Group Os07g0592600, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 7|Chromosome 7]]|
AP = Chromosome 7:24809875..24812231|
CDS = 24810162..24811536,24811631..24812073|
GCID = <gbrowseImage1>
name=NC_008400:24809875..24812231
source=RiceChromosome07
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008400:24809875..24812231
source=RiceChromosome07
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggtgatgactgatgtgtcgaccaccgggacggcactccggaccccggcggcgggggcggtgaaggagggcgacgtcgagaagctccggttcatcgacgagatgaccaccaacgtcgacgccgtgcaggagcgcgtcctgggggagatcctcggccgcaacgccggcacggagtacctgaccaagtgcggcctcgacggcgccaccgaccgcgccgccttccgggccaaggttccggtggtgtcgtacgacgacctgcagccgtacatccagcgcattgcgaacggggaccgctcgccgatcctgtccacccaccccgtctccgagttcctcaccagctccggcacgtcggccggcgagcgcaagctgatgcccaccatcatggacgagctcgaccgccgtcagctgctctacagcctcctcatgccggtcatgaacttgtatgtgcctgggcttgacaaggggaaggggctctacttcctgttcgtcaagtcggagacgaagacgccgggaggcctgacggcgaggcccgtgctgacgagctactacaagagcgaccacttcaagaaccggccgtacgacccgtaccacaactacacgagcccgacggcggccatcctctgcgccgacgcgttccagagcatgtacgcgcagatggtgtgcggcctgtgccagcgcaacgacgtgctgcgcctcggcgccgtgttcgcctccggcctcctccgggccatccgcttcctccagctcaactgggagcagctcgccgacgacatcgagtccggcgagctcacccctcgcgtcaccgacccgtcggtgcgcgaggctgtcgccgccatcctcctcccggaccccgagctcgccaagctcatccgcgccgagtgctccaagggcgactgggccggcatcatcacccgcgtgtggccgaacaccaagtacctcgacgtcatcgtcaccggcgcgatggcgcagtacatcccgaccctggagttctacagcggcgggcttcccatggcgtgcaccatgtacgcgtcatccgagtgctacttcggcctcaacctccgccccatgtgcgacccctccgaggtgtcctacaccatcatgcctaacatgggctacttcgagttcctccccgtggacgagaccggcgcggcttcgggcgacgccacccagctcgtggacctcgcccgcgtggaggtggggcgcgagtacgagctggtgatcaccacctacgccgggctgaaccggtaccgcgtcggcgacgtcctccgcgtgacggggttccacaacgcggcgccgcagttcaggttcgtgcgccgcaagaacgtgctcctctccatcgagtccgacaagaccgacgaggccgagctgcagcgcgccgtggagcgcgcgtcggcgctgctccgcccgcacggcgcgtccgtggtggagtacaccagccaagcgtgcaccaagcgcatcccgggccactacgtcatctactgggagctcctgaccaagggcgccggcgccacggtggtggacgcggacacgctgggcaggtgctgcctcgagatggaggaggcgctgaacacggtgtacaggcagagccgcgtcgcggacggctcgattgggccgctcgagatccgggtcgtccgcccgggcacattcgaggagctcatggactacgccatctcccgcggcgcgtccatcaaccagtacaaggtgccacgctgcgtcacgttcccgcccatcgtcgagctgctcgactcgcgcgtcgtgtccagccacttcagcccggcgctcccacactggactccggcgcgacggtccgaatag</cdnaseq>|
AA = <aaseq>MAVMTDVSTTGTALRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQ ERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYDDLQPYIQRIANGDRSPI LSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLYVPGLDKGKGLY FLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILCADAFQSMYA QMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVTDPSVREA VAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIPTLEFYSG GLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGAASGDATQL VDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSIESD KTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKGAGATVVD ADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGASINQ YKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARRSE</aaseq>|
DNA = <dnaseqindica>696..2070#159..601#aggccacacaccaccaacacaaaacatttccttgtccctcgccttcctcgcctcgcctcggctctcacacgacgcctcttcctctcgctactactagctcaagctaagctagcaagagcattccttgggctttttttcttgttcggggttgagaggcaatggcggtgatgactgatgtgtcgaccaccgggacggcactccggaccccggcggcgggggcggtgaaggagggcgacgtcgagaagctccggttcatcgacgagatgaccaccaacgtcgacgccgtgcaggagcgcgtcctgggggagatcctcggccgcaacgccggcacggagtacctgaccaagtgcggcctcgacggcgccaccgaccgcgccgccttccgggccaaggttccggtggtgtcgtacgacgacctgcagccgtacatccagcgcattgcgaacggggaccgctcgccgatcctgtccacccaccccgtctccgagttcctcaccagctccggcacgtcggccggcgagcgcaagctgatgcccaccatcatggacgagctcgaccgccgtcagctgctctacagcctcctcatgccggtcatgaacttgtaagttgatactactcctatcattgtctcattctgatagcacacacacgtacaagaagcagatgatgattaatggccatgaatcattgcataggtatgtgcctgggcttgacaaggggaaggggctctacttcctgttcgtcaagtcggagacgaagacgccgggaggcctgacggcgaggcccgtgctgacgagctactacaagagcgaccacttcaagaaccggccgtacgacccgtaccacaactacacgagcccgacggcggccatcctctgcgccgacgcgttccagagcatgtacgcgcagatggtgtgcggcctgtgccagcgcaacgacgtgctgcgcctcggcgccgtgttcgcctccggcctcctccgggccatccgcttcctccagctcaactgggagcagctcgccgacgacatcgagtccggcgagctcacccctcgcgtcaccgacccgtcggtgcgcgaggctgtcgccgccatcctcctcccggaccccgagctcgccaagctcatccgcgccgagtgctccaagggcgactgggccggcatcatcacccgcgtgtggccgaacaccaagtacctcgacgtcatcgtcaccggcgcgatggcgcagtacatcccgaccctggagttctacagcggcgggcttcccatggcgtgcaccatgtacgcgtcatccgagtgctacttcggcctcaacctccgccccatgtgcgacccctccgaggtgtcctacaccatcatgcctaacatgggctacttcgagttcctccccgtggacgagaccggcgcggcttcgggcgacgccacccagctcgtggacctcgcccgcgtggaggtggggcgcgagtacgagctggtgatcaccacctacgccgggctgaaccggtaccgcgtcggcgacgtcctccgcgtgacggggttccacaacgcggcgccgcagttcaggttcgtgcgccgcaagaacgtgctcctctccatcgagtccgacaagaccgacgaggccgagctgcagcgcgccgtggagcgcgcgtcggcgctgctccgcccgcacggcgcgtccgtggtggagtacaccagccaagcgtgcaccaagcgcatcccgggccactacgtcatctactgggagctcctgaccaagggcgccggcgccacggtggtggacgcggacacgctgggcaggtgctgcctcgagatggaggaggcgctgaacacggtgtacaggcagagccgcgtcgcggacggctcgattgggccgctcgagatccgggtcgtccgcccgggcacattcgaggagctcatggactacgccatctcccgcggcgcgtccatcaaccagtacaaggtgccacgctgcgtcacgttcccgcccatcgtcgagctgctcgactcgcgcgtcgtgtccagccacttcagcccggcgctcccacactggactccggcgcgacggtccgaatagtcggtcaaatccccacctcgtcgttggaccgtgtccaagaatctgaagtagtagaagccggatgcctccacctaaaaacacttatctgttatttttggaattaattttgatggttgctgtcaactctgtcagttagtggcaagagggtacctctgatgtgagggagagaactgcagaacgaacggaagaaagtgttgatgtaagaggttaccactagtagtactgtttgtctgtatcaggaatgtgattataatttaacctgtcctccaaaaccgtacgagtcctgc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001066698.1 RefSeq:Os07g0592600]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 7]]
[[Category:Chromosome 7]]

Os12g0189500

2014-06-03T14:17:41Z

Hylwayne: /* Evolution */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.
The increase in IAA content was strongly correlated with the expression of putative
IAA biosynthesis genes, OsYUC9, OsYUC11 and OsTAR1, measured by
quantitative reverse transcriptase polymerase chain reaction<ref name="pmid:16660061" />. These results
conﬁrm the importance of the tryptophan aminotransferase/YUCCA pathway
in this system. All three genes were expressed in endosperm; expression
of OsYUC11 appeared to be conﬁned to endosperm tissue<ref name="pmid:17889819" />. Phylogenetic
analysis indicated that OsYUC11 and AtYUC10 belong to a separate clade of
YUCCAs, which do not have orthologues outside the Angiosperms<ref name="pmid:18410479" />. This clade
may have evolved with a speciﬁc role in endosperm. Expression of tryptophan
decarboxylase in developing rice grains did not correlate with IAA levels,
indicating that tryptamine is unlikely to be important for IAA synthesis in this
system<ref name="pmid:16665995" />. In light of these observations, we hypothesize that IAA production
in developing rice grains is controlled via expression of OsTAR1, OsYUC9,
OsYUC11 and that IAA may be important during starch deposition in addition
to its pr eviously suggested role early in grain development.

===Expression===
Quantitative RT–PCR results for OsYUC3, OsYUC 9, OsYUC 11, OsYUC 12, OsYUC 14, OsTAR1, OsTAR2 and OsTDC1. Total RNA was extracted using a Qiagen RNeasy Kit. Reaction tubes were prepared using a CAS-1200 Robotic liquid-handling system, Qiagen. Each tube contained 250 ng
RNA per 25 μL reaction and a ﬁnal primer concentration of 0.5 μM. Ampliﬁcation was carried out using a Qiagen QuantiTect SYBR Green RT–PCR kit in a Corbett Research Rotor-Gene 3000 thermocycler ﬁtted with a 72-tube rotor for 45 cycles. Expression was calculated relative to reference genes UBC using ROTORGENE software (Qiagen) <ref name="pmid: 16690022 " />. Melt curve analysis conﬁrmed that a single product was obtained for each gene ampliﬁed. Results shown are the means ± the standard error of three biological replicates and two technical replicates. Comparison of the expression of YUCCA, TAA/TAR and TDC orthologues during grain/seed development <ref name="pmid: 17293439 " />.

===Evolution===
Please input evolution information here.

Phylogenetic tree of FMOs from rice (Os), Arabidopsis (At), Selaginella (Smoe), Physcomitrella (Ppls) and Chlamydomonas (Cr/Cre). Human FMOs, translated Pinus cDNAs and four sequences from bacteria, representing the most closely related proteins to YUCCA from outside the plant kingdom were also included. Multiple sequence alignments were carried out using MUSCLE (Edgar 2004); trees were constructed using PROTDIST and FITSCH bootstrap values for major branches are indicated. Scale bar indicates 0.1 amino acid substitutions persite<ref name="pmid: 19117763" />. Examination of the expression of YUCCA in developing seeds using both RT–PCR and microarray data indicated that OsYUC11 and AtYUC10 appeared to be speciﬁc to, and highly expressed in, endosperm tissue. These two proteins are orthologues of each other and also of ZmYUC, which appears to be important for IAA synthesis in developing maize kernels.
You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

Present address: Department of applied biological science, Al-Ghad International AppliedMedical Science University, Jeddah, Saudi Arabia

==References==
<references>

<ref name="pmid:16660061">Bandurski RS, Schulze A (1977) Concentration of indole-3-acetic acid and its derivatives in plants. Plant Physiol 60: 211–213.</ref>
<ref name="pmid:17889819"> Barkawi LS, Tam YY, Tillman JA, Pederson B, Calio J,Al-Amier H, Emerick M, Normanly J, Cohen JD (2008) A high-throughput method for the quantitative analysis of indole-3-acetic acid and other auxins from plant tissue. Anal Biochem 372: 177–188 </ref>
<ref name="pmid:18410479 "> Benedito VA, Torres-Jerez I, Murray JD, Andriankaja A,Allen S, Kakar K, Wandrey M, Verdier J, Zuber H, Ott T,Moreau S, Niebel A, Frickey T, Weiller G, He J, Dai XB,Zhao PX, Tang YH, Udvardi MK (2008) A gene expression atlas of the model legume Medicago truncatula. Plant J 55: 504–513. </ref>
<ref name="pmid:16665995 ">Chen KH, Miller AN, Patterson GW, Cohen JD (1988) A Rapid and simple procedure for puriﬁcation of indole-3-acetic-acid prior to GC-SIM-MS analysis. Plant Physiol 86: 822–825 </ref>
<ref name="pmid: 16690022"> Jain M, Nijhawan A, Tyagi AK, Khurana JP (2006) Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Biochem Biophys Res Commun 345: 646–651.</ref>
<ref name="pmid: 17293439"> Jain M, Nijhawan A, Arora R, Agarwal P, Ray S, Sharma P, Kapoor S, Tyagi AK, Khurana JP (2007) F-box proteins in rice. genome-wide analysis, classiﬁcation, temporal and spatial gene expression during panicle and seed development, and regulation by light and abiotic stress.Plant Physiol 143: 1467–1483.</ref>
<ref name="pmid: 19117763"> Zhang H, Tan GLL, Yang LNN, Yang JC, Zhang JH, Zhao BH (2009) Hormones in the grains and roots in relation to post-anthesis development of inferior and superior spikelets in japonica/indica hybrid rice. Plant Physiol Biochem 47: 195–204.</ref>

</references>

Please input cited references here.
{{JaponicaGene|
GeneName = Os12g0189500|
Description = Pyridine nucleotide-disulphide oxidoreductase, class I family protein|
Version = NM_001072867.2 GI:297612801 GeneID:4351695|
Length = 8650 bp|
Definition = Oryza sativa Japonica Group Os12g0189500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 12|Chromosome 12]]|
AP = Chromosome 12:4507750..4516399|
CDS = 4507750..4507887,4514763..4514909,4515209..4515331,4515422..4515658,4515788..4516399 |
GCID = <gbrowseImage1>
name=NC_008405:4507750..4516399
source=RiceChromosome12
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008405:4507750..4516399
source=RiceChromosome12
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggagaaggctctagttctgattgttggagcaggcccatctggtcttgcaacagcggcgtgccttggccagctctctataccctatgttattatcgagcgtgaggattgtaccgcgtcgctgtggcgtaagcacacatatgatcgcctcaagcttcacctcgcaaaggagttctgcgagatgccccacatgccatatccggaagacacaccaacctacatccccaagattcaatttttgaggtatatggatgattatgtggagcatttcaacatttgtcccaaatttaactcatctgttgagtcatgtttgtatgatgatgtgcaaaaatattgggttgtcacaacacatgaccaagtgaatggcatggtgtccaagtatgcagctaggttcttagttgtagcaagtggtgaaaatagtgcagggaacattcctagcatccctggactagaggatttctctggccatgtcatacactcatcaagctttaggtcagcagattcctatgctgcgcaaagggtgcttgttgttggatgtggaaactcaggcatggagattgcctatgatctttcaagccatggagcaaatacctccatagtcatccgcagccctctgcatgtgatgacaaaggagctgattcacatggggatgaaacttgcaagctggagcctcccagtgaaatttgttgattttattcttgtggtccttgcatatctctggtttggcaatctctcaaagtatggcatcgtgaggcccaataaggggccattgcttcttaaggcaaatactggtcggtcagcagtaatagatgttggcactgtggagttaataaagaaaggggatatcaaggtctttggtacaatatcttgcatcaagggaaatgttgttgaatttgatgatgggaaagaaagttactttgatgctattgtttttgctacaggctacacaagtactgcaaacaactggcttaagaatggtgaggatatgatgaacaaagaaggcatgcccaagaaggacttcccaaatcactggaaaggttcaaatggactctactgtgttgggtttgcgaggagaggattatctggtattgctcatgacgccaaaaacgttgctaatgactggatggaagaagacttgacaccaaagcccgccgttggcagttctaagaacagtcagccctcacttatcacatccatggatggtgaacgatgctttgccttagtggcagagcaaactgtttccattggaggcaattga</cdnaseq>|
AA = <aaseq>MEKALVLIVGAGPSGLATAACLGQLSIPYVIIEREDCTASLWRK HTYDRLKLHLAKEFCEMPHMPYPEDTPTYIPKIQFLRYMDDYVEHFNICPKFNSSVES CLYDDVQKYWVVTTHDQVNGMVSKYAARFLVVASGENSAGNIPSIPGLEDFSGHVIHS SSFRSADSYAAQRVLVVGCGNSGMEIAYDLSSHGANTSIVIRSPLHVMTKELIHMGMK LASWSLPVKFVDFILVVLAYLWFGNLSKYGIVRPNKGPLLLKANTGRSAVIDVGTVEL IKKGDIKVFGTISCIKGNVVEFDDGKESYFDAIVFATGYTSTANNWLKNGEDMMNKEG MPKKDFPNHWKGSNGLYCVGFARRGLSGIAHDAKNVANDWMEEDLTPKPAVGSSKNSQ PSLITSMDGERCFALVAEQTVSIGGN</aaseq>|
DNA = <dnaseqindica>8513..8650#1491..1637#1069..1191#742..978#1..612#atggagaaggctctagttctgattgttggagcaggcccatctggtcttgcaacagcggcgtgccttggccagctctctataccctatgttattatcgagcgtgaggattgtaccgcgtcgctgtggcgtaagcacacatatgatcgcctcaagcttcacctcgcaaaggagttctgcgagatgccccacatgccatatccggaagacacaccaacctacatccccaagattcaatttttgaggtatatggatgattatgtggagcatttcaacatttgtcccaaatttaactcatctgttgagtcatgtttgtatgatgatgtgcaaaaatattgggttgtcacaacacatgaccaagtgaatggcatggtgtccaagtatgcagctaggttcttagttgtagcaagtggtgaaaatagtgcagggaacattcctagcatccctggactagaggatttctctggccatgtcatacactcatcaagctttaggtcagcagattcctatgctgcgcaaagggtgcttgttgttggatgtggaaactcaggcatggagattgcctatgatctttcaagccatggagcaaatacctccatagtcatccgcagccctgtaagtaaataatacatttgcataaaagtttcctttctttattttgcctccctgttttccaggtcaagttattgtaagtatcctattaaatcattttcttacaccacaaattcttgaattattctaaagctgcatgtgatgacaaaggagctgattcacatggggatgaaacttgcaagctggagcctcccagtgaaatttgttgattttattcttgtggtccttgcatatctctggtttggcaatctctcaaagtatggcatcgtgaggcccaataaggggccattgcttcttaaggcaaatactggtcggtcagcagtaatagatgttggcactgtggagttaataaagaaaggggatatcaaggtaacaaaattcttgacacaacatagaaaagtctatataaattctatttggtacttgacaggttctataaggaatttatgtggcatgtaggtctttggtacaatatcttgcatcaagggaaatgttgttgaatttgatgatgggaaagaaagttactttgatgctattgtttttgctacaggctacacaagtactgcaaacaactggcttaaggtatgtaatatgttgctcatatggtaaatagtaaatacaaaaattattgcaattagatttagttgttattttctctctcctgcatacctcaagtggggcatcaaattaagagtgcctacaatactattagacagatttagaaagaaaagcatttgcgtgattgactcaacaaaattagtgataaaacctgttataaaattatagttataattagaagacaatggtgctatcttcatatttaactttctttctttctgtggaaatatttgttaataaaataattatgattaaaaacgcagaatggtgaggatatgatgaacaaagaaggcatgcccaagaaggacttcccaaatcactggaaaggttcaaatggactctactgtgttgggtttgcgaggagaggattatctggtattgctcatgacgccaaaaacgttgctaatgacgtcaaggccttcctagatttgatggcaccattctaattattatttgcactggaatgatgcctatcaaatagtattgtggtaccttctacaatagtggtgtgtacttaatgttacattatgtttgaagcaagctaaatgttatgcaatctccagagttgtttaaataattatattttcttaatcgacatgttggatgagcttttatttaaagctctttgctggaatacatgaatatgtggtttattctttataatgtgtgaagccctttgtttggatccatgaaaaataaaatcagaaactcatgtcaaacctcccctcacacctgtattacctatcctactctaaatcataccccctcctccccccatcggcaccatagttgttcagccagtgacgccctgcccttttgaaagaagttgagcatcactgccggttcatggctgtttaggcgggaaaattcactccaacgccatgaactggtaaagattggcaatccctgttggctcacaccgaaccgatagagatcagtgggcaaggattgccctttctgtaccagtaacacctttatccactccagttatgaaaaaaaaatgctctttagagttgtttgttccttatttcaactcaacacaacaatatggagttctaaaaaataacacaacattatggagaatactaaataatagtagagatcatagattacttagaagttagaacatacctaaaagggagattttttttttaaaaaaagaattcattttcaatcctatgccttattttcactgacatttggaaagggagtagtatgtctatttggggaagaggttcttttttttttttggggggcggggggggggggggggagggggaagggagggctgctttgattaattcctcccattgtattacatttcacttggatggatcatacctcctattcttctagataccacgaacaaatgtcattgaattatgagatagactaaaatctgcgggtgtcaaagatttcagaaaacttgagtctttgatgtaaatgttggtggaaattgcacccttttatatcaaatgcccattgggtttgtgcagctagcctaaaaaagattattccccattttggaaggttatgtgttacaaatcagacatctccgcaatattttcagaaaactaaagtgggtactaatactatatttttgcatgatcctggcctaaggtggtattacaatctgttgagatatgcaatggtcaaagagtggtactgtacattctattagagagctagattttattttgaactctagatgtgggtaactgatgatactggattgacgaaagccgaaagcccatctttatgagttttttaaggacttggtaggatcaatatataaacttttaataggagaaaatgtacatttactataaccaatttttacatgacgcctcgacaggaggcacccctcccctaggcatgcctctaaagttagccctgttagtgttctatcccttttgagcagaccattcttgagtaggttacaaatgtcacactgtttgatactccatttgtcctaaaatataaggtggccatatatagttttagcaaaaccaacatatgtaggttttgacaaataacaaactaacaatatactgtatatgtttaatgccatccatttcatatcctaagattcatatttgaaagcactcttgtatgatgtaattttctagttcttgataatatattattgaagaaactaattgttaaaatataatattgtagacagtgtaagaaaactaatatgctttatagtttgagactgataggactattataaaaaggtcctcctattacacttgatgagaatgatggtaaaattgcatgatatataatttgtgaaagctggcacatattcaatgaaatcgatatattctttggatgatcacgggtgtttctgggccaaatttctactttatttgtaatcccgagagcttcatctacaagtaaaaaaaaaatccaggtttttagttgataatgtagtctatcttaataaaggaagagatgcaaaggaggaaatttcctgcaaacaccggaatgtcttttcttccctgactgaaaatacagttttgttctttacatgtaaattagctagcaaagttatttaggggcattacaggtttagatatgctttggcaccaaaaccaggtacagaacaaatgttcgctggatgcactgcatcgatcttgatgtactccctatgttcaattatataagacatttttggtttgttctaatcaaacttgaatatacatattttcagtttgaccaaacaatgtagtagtgtttccaacacaaaacaaatatactacagaaatatatttaatggtggatttaatgaaaccaataaagagtatgcgataatagtaagtccatctaatgcataaaatcctggtgcattactatcctaggttaatatggcaccacctatactaagatcttcttctttctcttcatgtttgattgtacatcttgatatttccttccatgtgcatgcatggtttcaacaaaaaaaaaggtaacactgacttgttccaataatacacattgtagttatctatagcccttttttacatacatattacccccattatgtttgcagttgttacccccctttatctgattagagcataaaggacctgtggaatttttactcttataacccgactttttttatctattccataacctacaaatgggtttcctattttgatttgtaaaaataacaaagagaaagcctttgctctgatggtcacaatccttcgatttattccttttgtttgtaataatgttagtaaagtaatccatcagttgatttataaccccctctcgccaatgaaactgattcacttttatgaagtgaggagaaagaagggatcaattaatcgaggatccaatattttagtccctcgaggaagtctcctaaatcattggtagtattcatttgactctactagttacaactaataagacatatttataagaaagaaaatgcagcaggaggattactgatttcaattagttactatgtgcaaaattcagtgctcgcgtgccggggggggggggggaggggcggcaacggagttattgttcctacatacaataaatagtgatgacatgctctagtgaagttatttcagactggatccacatgggtgtctttcttttgtttctttggaaaggcttgttgtagctatctcatcttgataggtcgaaagattcgttcgttagtaacgacttgcttaaacaaagtt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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001072867.2 RefSeq:Os12g0189500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 12]]
[[Category:Chromosome 12]]

Os12g0189500

2014-06-03T14:16:52Z

Hylwayne: /* Evolution */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.
The increase in IAA content was strongly correlated with the expression of putative
IAA biosynthesis genes, OsYUC9, OsYUC11 and OsTAR1, measured by
quantitative reverse transcriptase polymerase chain reaction<ref name="pmid:16660061" />. These results
conﬁrm the importance of the tryptophan aminotransferase/YUCCA pathway
in this system. All three genes were expressed in endosperm; expression
of OsYUC11 appeared to be conﬁned to endosperm tissue<ref name="pmid:17889819" />. Phylogenetic
analysis indicated that OsYUC11 and AtYUC10 belong to a separate clade of
YUCCAs, which do not have orthologues outside the Angiosperms<ref name="pmid:18410479" />. This clade
may have evolved with a speciﬁc role in endosperm. Expression of tryptophan
decarboxylase in developing rice grains did not correlate with IAA levels,
indicating that tryptamine is unlikely to be important for IAA synthesis in this
system<ref name="pmid:16665995" />. In light of these observations, we hypothesize that IAA production
in developing rice grains is controlled via expression of OsTAR1, OsYUC9,
OsYUC11 and that IAA may be important during starch deposition in addition
to its pr eviously suggested role early in grain development.

===Expression===
Quantitative RT–PCR results for OsYUC3, OsYUC 9, OsYUC 11, OsYUC 12, OsYUC 14, OsTAR1, OsTAR2 and OsTDC1. Total RNA was extracted using a Qiagen RNeasy Kit. Reaction tubes were prepared using a CAS-1200 Robotic liquid-handling system, Qiagen. Each tube contained 250 ng
RNA per 25 μL reaction and a ﬁnal primer concentration of 0.5 μM. Ampliﬁcation was carried out using a Qiagen QuantiTect SYBR Green RT–PCR kit in a Corbett Research Rotor-Gene 3000 thermocycler ﬁtted with a 72-tube rotor for 45 cycles. Expression was calculated relative to reference genes UBC using ROTORGENE software (Qiagen) <ref name="pmid: 16690022 " />. Melt curve analysis conﬁrmed that a single product was obtained for each gene ampliﬁed. Results shown are the means ± the standard error of three biological replicates and two technical replicates. Comparison of the expression of YUCCA, TAA/TAR and TDC orthologues during grain/seed development <ref name="pmid: 17293439 " />.

===Evolution===
Please input evolution information here.

Phylogenetic tree of FMOs from rice (Os), Arabidopsis (At), Selaginella (Smoe), Physcomitrella (Ppls) and Chlamydomonas (Cr/Cre). Human FMOs, translated Pinus cDNAs and four sequences from bacteria, representing the most closely related proteins to YUCCA from outside the plant kingdom were also included. Multiple sequence alignments were carried out using MUSCLE (Edgar 2004); trees were constructed using PROTDIST and FITSCH bootstrap values for major branches are indicated. Scale bar indicates 0.1 amino acid substitutions persite<ref name="pmid: 19117763">. Examination of the expression of YUCCA in developing seeds using both RT–PCR and microarray data indicated that OsYUC11 and AtYUC10 appeared to be speciﬁc to, and highly expressed in, endosperm tissue. These two proteins are orthologues of each other and also of ZmYUC, which appears to be important for IAA synthesis in developing maize kernels.
You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

Present address: Department of applied biological science, Al-Ghad International AppliedMedical Science University, Jeddah, Saudi Arabia

==References==
<references>

<ref name="pmid:16660061">Bandurski RS, Schulze A (1977) Concentration of indole-3-acetic acid and its derivatives in plants. Plant Physiol 60: 211–213.</ref>
<ref name="pmid:17889819"> Barkawi LS, Tam YY, Tillman JA, Pederson B, Calio J,Al-Amier H, Emerick M, Normanly J, Cohen JD (2008) A high-throughput method for the quantitative analysis of indole-3-acetic acid and other auxins from plant tissue. Anal Biochem 372: 177–188 </ref>
<ref name="pmid:18410479 "> Benedito VA, Torres-Jerez I, Murray JD, Andriankaja A,Allen S, Kakar K, Wandrey M, Verdier J, Zuber H, Ott T,Moreau S, Niebel A, Frickey T, Weiller G, He J, Dai XB,Zhao PX, Tang YH, Udvardi MK (2008) A gene expression atlas of the model legume Medicago truncatula. Plant J 55: 504–513. </ref>
<ref name="pmid:16665995 ">Chen KH, Miller AN, Patterson GW, Cohen JD (1988) A Rapid and simple procedure for puriﬁcation of indole-3-acetic-acid prior to GC-SIM-MS analysis. Plant Physiol 86: 822–825 </ref>
<ref name="pmid: 16690022"> Jain M, Nijhawan A, Tyagi AK, Khurana JP (2006) Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Biochem Biophys Res Commun 345: 646–651.</ref>
<ref name="pmid: 17293439"> Jain M, Nijhawan A, Arora R, Agarwal P, Ray S, Sharma P, Kapoor S, Tyagi AK, Khurana JP (2007) F-box proteins in rice. genome-wide analysis, classiﬁcation, temporal and spatial gene expression during panicle and seed development, and regulation by light and abiotic stress.Plant Physiol 143: 1467–1483.</ref>
<ref name="pmid: 19117763"> Zhang H, Tan GLL, Yang LNN, Yang JC, Zhang JH, Zhao BH (2009) Hormones in the grains and roots in relation to post-anthesis development of inferior and superior spikelets in japonica/indica hybrid rice. Plant Physiol Biochem 47: 195–204.</ref>

</references>

Please input cited references here.
{{JaponicaGene|
GeneName = Os12g0189500|
Description = Pyridine nucleotide-disulphide oxidoreductase, class I family protein|
Version = NM_001072867.2 GI:297612801 GeneID:4351695|
Length = 8650 bp|
Definition = Oryza sativa Japonica Group Os12g0189500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 12|Chromosome 12]]|
AP = Chromosome 12:4507750..4516399|
CDS = 4507750..4507887,4514763..4514909,4515209..4515331,4515422..4515658,4515788..4516399 |
GCID = <gbrowseImage1>
name=NC_008405:4507750..4516399
source=RiceChromosome12
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008405:4507750..4516399
source=RiceChromosome12
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggagaaggctctagttctgattgttggagcaggcccatctggtcttgcaacagcggcgtgccttggccagctctctataccctatgttattatcgagcgtgaggattgtaccgcgtcgctgtggcgtaagcacacatatgatcgcctcaagcttcacctcgcaaaggagttctgcgagatgccccacatgccatatccggaagacacaccaacctacatccccaagattcaatttttgaggtatatggatgattatgtggagcatttcaacatttgtcccaaatttaactcatctgttgagtcatgtttgtatgatgatgtgcaaaaatattgggttgtcacaacacatgaccaagtgaatggcatggtgtccaagtatgcagctaggttcttagttgtagcaagtggtgaaaatagtgcagggaacattcctagcatccctggactagaggatttctctggccatgtcatacactcatcaagctttaggtcagcagattcctatgctgcgcaaagggtgcttgttgttggatgtggaaactcaggcatggagattgcctatgatctttcaagccatggagcaaatacctccatagtcatccgcagccctctgcatgtgatgacaaaggagctgattcacatggggatgaaacttgcaagctggagcctcccagtgaaatttgttgattttattcttgtggtccttgcatatctctggtttggcaatctctcaaagtatggcatcgtgaggcccaataaggggccattgcttcttaaggcaaatactggtcggtcagcagtaatagatgttggcactgtggagttaataaagaaaggggatatcaaggtctttggtacaatatcttgcatcaagggaaatgttgttgaatttgatgatgggaaagaaagttactttgatgctattgtttttgctacaggctacacaagtactgcaaacaactggcttaagaatggtgaggatatgatgaacaaagaaggcatgcccaagaaggacttcccaaatcactggaaaggttcaaatggactctactgtgttgggtttgcgaggagaggattatctggtattgctcatgacgccaaaaacgttgctaatgactggatggaagaagacttgacaccaaagcccgccgttggcagttctaagaacagtcagccctcacttatcacatccatggatggtgaacgatgctttgccttagtggcagagcaaactgtttccattggaggcaattga</cdnaseq>|
AA = <aaseq>MEKALVLIVGAGPSGLATAACLGQLSIPYVIIEREDCTASLWRK HTYDRLKLHLAKEFCEMPHMPYPEDTPTYIPKIQFLRYMDDYVEHFNICPKFNSSVES CLYDDVQKYWVVTTHDQVNGMVSKYAARFLVVASGENSAGNIPSIPGLEDFSGHVIHS SSFRSADSYAAQRVLVVGCGNSGMEIAYDLSSHGANTSIVIRSPLHVMTKELIHMGMK LASWSLPVKFVDFILVVLAYLWFGNLSKYGIVRPNKGPLLLKANTGRSAVIDVGTVEL IKKGDIKVFGTISCIKGNVVEFDDGKESYFDAIVFATGYTSTANNWLKNGEDMMNKEG MPKKDFPNHWKGSNGLYCVGFARRGLSGIAHDAKNVANDWMEEDLTPKPAVGSSKNSQ PSLITSMDGERCFALVAEQTVSIGGN</aaseq>|
DNA = <dnaseqindica>8513..8650#1491..1637#1069..1191#742..978#1..612#atggagaaggctctagttctgattgttggagcaggcccatctggtcttgcaacagcggcgtgccttggccagctctctataccctatgttattatcgagcgtgaggattgtaccgcgtcgctgtggcgtaagcacacatatgatcgcctcaagcttcacctcgcaaaggagttctgcgagatgccccacatgccatatccggaagacacaccaacctacatccccaagattcaatttttgaggtatatggatgattatgtggagcatttcaacatttgtcccaaatttaactcatctgttgagtcatgtttgtatgatgatgtgcaaaaatattgggttgtcacaacacatgaccaagtgaatggcatggtgtccaagtatgcagctaggttcttagttgtagcaagtggtgaaaatagtgcagggaacattcctagcatccctggactagaggatttctctggccatgtcatacactcatcaagctttaggtcagcagattcctatgctgcgcaaagggtgcttgttgttggatgtggaaactcaggcatggagattgcctatgatctttcaagccatggagcaaatacctccatagtcatccgcagccctgtaagtaaataatacatttgcataaaagtttcctttctttattttgcctccctgttttccaggtcaagttattgtaagtatcctattaaatcattttcttacaccacaaattcttgaattattctaaagctgcatgtgatgacaaaggagctgattcacatggggatgaaacttgcaagctggagcctcccagtgaaatttgttgattttattcttgtggtccttgcatatctctggtttggcaatctctcaaagtatggcatcgtgaggcccaataaggggccattgcttcttaaggcaaatactggtcggtcagcagtaatagatgttggcactgtggagttaataaagaaaggggatatcaaggtaacaaaattcttgacacaacatagaaaagtctatataaattctatttggtacttgacaggttctataaggaatttatgtggcatgtaggtctttggtacaatatcttgcatcaagggaaatgttgttgaatttgatgatgggaaagaaagttactttgatgctattgtttttgctacaggctacacaagtactgcaaacaactggcttaaggtatgtaatatgttgctcatatggtaaatagtaaatacaaaaattattgcaattagatttagttgttattttctctctcctgcatacctcaagtggggcatcaaattaagagtgcctacaatactattagacagatttagaaagaaaagcatttgcgtgattgactcaacaaaattagtgataaaacctgttataaaattatagttataattagaagacaatggtgctatcttcatatttaactttctttctttctgtggaaatatttgttaataaaataattatgattaaaaacgcagaatggtgaggatatgatgaacaaagaaggcatgcccaagaaggacttcccaaatcactggaaaggttcaaatggactctactgtgttgggtttgcgaggagaggattatctggtattgctcatgacgccaaaaacgttgctaatgacgtcaaggccttcctagatttgatggcaccattctaattattatttgcactggaatgatgcctatcaaatagtattgtggtaccttctacaatagtggtgtgtacttaatgttacattatgtttgaagcaagctaaatgttatgcaatctccagagttgtttaaataattatattttcttaatcgacatgttggatgagcttttatttaaagctctttgctggaatacatgaatatgtggtttattctttataatgtgtgaagccctttgtttggatccatgaaaaataaaatcagaaactcatgtcaaacctcccctcacacctgtattacctatcctactctaaatcataccccctcctccccccatcggcaccatagttgttcagccagtgacgccctgcccttttgaaagaagttgagcatcactgccggttcatggctgtttaggcgggaaaattcactccaacgccatgaactggtaaagattggcaatccctgttggctcacaccgaaccgatagagatcagtgggcaaggattgccctttctgtaccagtaacacctttatccactccagttatgaaaaaaaaatgctctttagagttgtttgttccttatttcaactcaacacaacaatatggagttctaaaaaataacacaacattatggagaatactaaataatagtagagatcatagattacttagaagttagaacatacctaaaagggagattttttttttaaaaaaagaattcattttcaatcctatgccttattttcactgacatttggaaagggagtagtatgtctatttggggaagaggttcttttttttttttggggggcggggggggggggggggagggggaagggagggctgctttgattaattcctcccattgtattacatttcacttggatggatcatacctcctattcttctagataccacgaacaaatgtcattgaattatgagatagactaaaatctgcgggtgtcaaagatttcagaaaacttgagtctttgatgtaaatgttggtggaaattgcacccttttatatcaaatgcccattgggtttgtgcagctagcctaaaaaagattattccccattttggaaggttatgtgttacaaatcagacatctccgcaatattttcagaaaactaaagtgggtactaatactatatttttgcatgatcctggcctaaggtggtattacaatctgttgagatatgcaatggtcaaagagtggtactgtacattctattagagagctagattttattttgaactctagatgtgggtaactgatgatactggattgacgaaagccgaaagcccatctttatgagttttttaaggacttggtaggatcaatatataaacttttaataggagaaaatgtacatttactataaccaatttttacatgacgcctcgacaggaggcacccctcccctaggcatgcctctaaagttagccctgttagtgttctatcccttttgagcagaccattcttgagtaggttacaaatgtcacactgtttgatactccatttgtcctaaaatataaggtggccatatatagttttagcaaaaccaacatatgtaggttttgacaaataacaaactaacaatatactgtatatgtttaatgccatccatttcatatcctaagattcatatttgaaagcactcttgtatgatgtaattttctagttcttgataatatattattgaagaaactaattgttaaaatataatattgtagacagtgtaagaaaactaatatgctttatagtttgagactgataggactattataaaaaggtcctcctattacacttgatgagaatgatggtaaaattgcatgatatataatttgtgaaagctggcacatattcaatgaaatcgatatattctttggatgatcacgggtgtttctgggccaaatttctactttatttgtaatcccgagagcttcatctacaagtaaaaaaaaaatccaggtttttagttgataatgtagtctatcttaataaaggaagagatgcaaaggaggaaatttcctgcaaacaccggaatgtcttttcttccctgactgaaaatacagttttgttctttacatgtaaattagctagcaaagttatttaggggcattacaggtttagatatgctttggcaccaaaaccaggtacagaacaaatgttcgctggatgcactgcatcgatcttgatgtactccctatgttcaattatataagacatttttggtttgttctaatcaaacttgaatatacatattttcagtttgaccaaacaatgtagtagtgtttccaacacaaaacaaatatactacagaaatatatttaatggtggatttaatgaaaccaataaagagtatgcgataatagtaagtccatctaatgcataaaatcctggtgcattactatcctaggttaatatggcaccacctatactaagatcttcttctttctcttcatgtttgattgtacatcttgatatttccttccatgtgcatgcatggtttcaacaaaaaaaaaggtaacactgacttgttccaataatacacattgtagttatctatagcccttttttacatacatattacccccattatgtttgcagttgttacccccctttatctgattagagcataaaggacctgtggaatttttactcttataacccgactttttttatctattccataacctacaaatgggtttcctattttgatttgtaaaaataacaaagagaaagcctttgctctgatggtcacaatccttcgatttattccttttgtttgtaataatgttagtaaagtaatccatcagttgatttataaccccctctcgccaatgaaactgattcacttttatgaagtgaggagaaagaagggatcaattaatcgaggatccaatattttagtccctcgaggaagtctcctaaatcattggtagtattcatttgactctactagttacaactaataagacatatttataagaaagaaaatgcagcaggaggattactgatttcaattagttactatgtgcaaaattcagtgctcgcgtgccggggggggggggggaggggcggcaacggagttattgttcctacatacaataaatagtgatgacatgctctagtgaagttatttcagactggatccacatgggtgtctttcttttgtttctttggaaaggcttgttgtagctatctcatcttgataggtcgaaagattcgttcgttagtaacgacttgcttaaacaaagttactccaattttggtccatgtgcatgcatcttaattttttttcttttgttttgaaatccggttgtcatagccatctcaatataggccaaaataagtttttttggtgtttctttccttattagccctaaaaataggcgacaaatgtccaaaattgcaccactgacctataaggatgggcccctagctaagaattaggtgcgactgagacgaggtaaagacctatatatatactaagacatgtaacaccttttttatttttctatttggaaatattcttttccctatttcataggaatgaactaaatttcaaaaaagaaaaagcacacaattactacaaaaaagtatgtgattgaaccattgaccaaagatggcgattattgtctttttcacttggcaaagtaggcaacataatccatctctcggccgccgcttggccatgtcaaactggcttagtgataaaggtcatccccttcgtcgattggctcacaagtgctagaaaaaggggaaaataggacatggcctttggcaggatgtgtttgttgtgtacttgcggtgtgggcggaaagtacaagaagcatgataattgggctgttcagtacataccgatgagtgaggcaacttgaggtagacaaagcaatgaagtgtatggctttggtggtaagattgagtagcctcgtcctccacatttgggtgaacaacaatgcttgatacatgaatatagaaaaagcattggacaatatgcataatggcatgaggcaataacactaacaagccacattgtagactgagatatatattgagtaccacaaccttgttgtaggtagcaaggtcagaacaacactgcaatacattgaaagacataattgactatctttcttggaattatgagcatgttgatacacattccactacagaacaaccattgttcttcaattacctgaataatttgatcctggcccattactggaaaaaataagatggatgagattgcatgcagtcatagttagtgctctggctaggttgctcgtgccaccaagatgcgagattagcacccgagagagtataatgtgtgaaggcacttcctgtgagctgagcctaagggagattacagtgcgtgaagttgcatctgaagtctcaggtctctcgcccacttatggttttgcataatcttcaggacttaacatatgtacctgatgcaagttcagctttgtagaagcataaaatatatgtgacacaagttcaatatgggcgcaactaaaaaacacaatactaactaattaaaagacgcttaattcttttgttcatcaagtctttgtaagagaacttgtcgatgcaaaaaaacataccaaatttggtcgttaacagaactgtacgaagtctttttcatgagagtttaagctaatgagatggctaataagcgagtgacctagtgcttatttctctgtgtcatattcagttgtgtgcgtggatgtatcacgtgacaagtgtttcgctcaacaacaatggtacatatatccaaatgacatagaatatgttgcttgaataaaacataactgatactcgcccaattggaaaattagtcaccgaagccctaccacagttggttttgttttgtacttttatttgagagaaatcaagagatagacaaggataaaagagtgaccagagcgatggtgattatagtgttaaattgtcgttaggaaatcactcaatgtttaaggggctgtttggttcatgatcctggtgaaaaggagcctggcctaatccctccctgtcacatggttgagtgttgtttggttcaaaggcctaaaggattttcaggatgcctgacttgaaccactgccttgcatcctttagcctgcgtccggcttcccattttggagctggcccgagcttcaggcacgtcaactccatgcatgcagttgcttgcgacattgtggtcggaataaaaaatttggcaatagtcccatctatattgattaaatcggaagtatgagcttgttttgattttgattcaaatccttagtttttttcctctaaattaatacttgttaattgctataattgaggagtacgagtttatcagtgttcattgttactgatagacaagagataatcgatgaatgatgaatcttccataacttaggtaagtgttttaccaacatatgtagtgtagtaaaaaaatgaattcaaccctttcgtgcttactataactaattaatttggttttctatttgctcctttaactctaaccctagctctatttattggcttgaacaaggtacatcttatttgaaatgaattgaatgaataaggcaaaaaagaaaaaaaatcattcttttggattcctggtatcctacaactctttttcacactacttacaaattctttttttcttggtcatggataaggctatacataaaatcaatgaatacgttaatatgtatctattaagttgatacatatcttaattaccaatgtgtaatcttgaactcaggctttcctagtcaagcatattttaccaaattctcaagtagctctcagacatacgtttttatcaatcatgttgctcaggtcactaaccaaacaagccctaagttgcaagaacacaaacacgactgatttcgtcaatgagcccaagtcgttggtgtttttaccagatgactcatcactatcggttcctttagagatgacactgatatcactgatggttcgtaattaattaacaagaaattactatgatacttaagtttccactaatgtatcacggttagttcataacaacaaccagcaccgatattcaatggaccagacctcaatggatcggatcatattcaagactatcatgtccattgagtatcaaactatatgtcattagtactcaaatgataatttgaaagaaaatggtaactttttcataagtcaggtgaagacaaacattatgtaaaagtatatagtttaaccaaagctacagctttgtagttgatgattttttgatttttgagattgtttacatgcccaagttgtcaaatctactcggacaaattttttgatattcccaaatgtccttggattgagactaactctacataaaaattggcgacacgatctattaatttttagttgacaatattttcattgtaagtcatttatatgcccgaatatatgttagaagtgttggctctatttggataaacgaaattttaaaatttttcaacgacttcagacaaacatgaactctatatggaagttgtggagatcgatgagatctacagatttgtcattgaagaccgaaaataatagcataagctatttaggcaaaaagagcattatagttgagatgggaggctgtggagatatagttgatcctatgtttgaatcctatgtacctcacacgcacgtacttcatgtgagaaatcatgtgaattgtgatttgtgatgtgtgtagatacttgatacgaaatacttttccaagtttttcatcgtggagagaattcaaaacccggtgcacaactatcaaatacatgttgatgtctttttgttgttctaactagtattaattgttcttaaataaagtggatggaagaagacttgacaccaaagcccgccgttggcagttctaagaacagtcagccctcacttatcacatccatggatggtgaacgatgctttgccttagtggcagagcaaactgtttccattggaggcaattga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001072867.2 RefSeq:Os12g0189500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 12]]
[[Category:Chromosome 12]]

Os12g0189500

2014-06-03T14:15:34Z

Hylwayne: /* Evolution */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.
The increase in IAA content was strongly correlated with the expression of putative
IAA biosynthesis genes, OsYUC9, OsYUC11 and OsTAR1, measured by
quantitative reverse transcriptase polymerase chain reaction<ref name="pmid:16660061" />. These results
conﬁrm the importance of the tryptophan aminotransferase/YUCCA pathway
in this system. All three genes were expressed in endosperm; expression
of OsYUC11 appeared to be conﬁned to endosperm tissue<ref name="pmid:17889819" />. Phylogenetic
analysis indicated that OsYUC11 and AtYUC10 belong to a separate clade of
YUCCAs, which do not have orthologues outside the Angiosperms<ref name="pmid:18410479" />. This clade
may have evolved with a speciﬁc role in endosperm. Expression of tryptophan
decarboxylase in developing rice grains did not correlate with IAA levels,
indicating that tryptamine is unlikely to be important for IAA synthesis in this
system<ref name="pmid:16665995" />. In light of these observations, we hypothesize that IAA production
in developing rice grains is controlled via expression of OsTAR1, OsYUC9,
OsYUC11 and that IAA may be important during starch deposition in addition
to its pr eviously suggested role early in grain development.

===Expression===
Quantitative RT–PCR results for OsYUC3, OsYUC 9, OsYUC 11, OsYUC 12, OsYUC 14, OsTAR1, OsTAR2 and OsTDC1. Total RNA was extracted using a Qiagen RNeasy Kit. Reaction tubes were prepared using a CAS-1200 Robotic liquid-handling system, Qiagen. Each tube contained 250 ng
RNA per 25 μL reaction and a ﬁnal primer concentration of 0.5 μM. Ampliﬁcation was carried out using a Qiagen QuantiTect SYBR Green RT–PCR kit in a Corbett Research Rotor-Gene 3000 thermocycler ﬁtted with a 72-tube rotor for 45 cycles. Expression was calculated relative to reference genes UBC using ROTORGENE software (Qiagen) <ref name="pmid: 16690022 " />. Melt curve analysis conﬁrmed that a single product was obtained for each gene ampliﬁed. Results shown are the means ± the standard error of three biological replicates and two technical replicates. Comparison of the expression of YUCCA, TAA/TAR and TDC orthologues during grain/seed development <ref name="pmid: 17293439 " />.

===Evolution===
Please input evolution information here.

Phylogenetic tree of FMOs from rice (Os), Arabidopsis (At), Selaginella (Smoe), Physcomitrella (Ppls) and Chlamydomonas (Cr/Cre). Human FMOs, translated Pinus cDNAs and four sequences from bacteria, representing the most closely related proteins to YUCCA from outside the plant kingdom were also included. Multiple sequence alignments were carried out using MUSCLE (Edgar 2004); trees were constructed using PROTDIST and FITSCH bootstrap values for major branches are indicated. Scale bar indicates 0.1 amino acid substitutions persite<ref name="pmid: 19117763">. Examination of the expression of YUCCA in developing seeds using both RT–PCR and microarray data indicated that OsYUC11 and AtYUC10 appeared to be speciﬁc to, and highly expressed in, endosperm tissue. These two proteins are orthologues of each other and also of ZmYUC, which appears to be important for IAA synthesis in developing maize kernels.
You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

Present address: Department of applied biological science, Al-Ghad International AppliedMedical Science University, Jeddah, Saudi Arabia

==References==
<references>

<ref name="pmid:16660061">Bandurski RS, Schulze A (1977) Concentration of indole-3-acetic acid and its derivatives in plants. Plant Physiol 60: 211–213.</ref>
<ref name="pmid:17889819"> Barkawi LS, Tam YY, Tillman JA, Pederson B, Calio J,Al-Amier H, Emerick M, Normanly J, Cohen JD (2008) A high-throughput method for the quantitative analysis of indole-3-acetic acid and other auxins from plant tissue. Anal Biochem 372: 177–188 </ref>
<ref name="pmid:18410479 "> Benedito VA, Torres-Jerez I, Murray JD, Andriankaja A,Allen S, Kakar K, Wandrey M, Verdier J, Zuber H, Ott T,Moreau S, Niebel A, Frickey T, Weiller G, He J, Dai XB,Zhao PX, Tang YH, Udvardi MK (2008) A gene expression atlas of the model legume Medicago truncatula. Plant J 55: 504–513. </ref>
<ref name="pmid:16665995 ">Chen KH, Miller AN, Patterson GW, Cohen JD (1988) A Rapid and simple procedure for puriﬁcation of indole-3-acetic-acid prior to GC-SIM-MS analysis. Plant Physiol 86: 822–825 </ref>
<ref name="pmid: 16690022"> Jain M, Nijhawan A, Tyagi AK, Khurana JP (2006) Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Biochem Biophys Res Commun 345: 646–651.</ref>

<ref name="pmid: 17293439"> Jain M, Nijhawan A, Arora R, Agarwal P, Ray S, Sharma P, Kapoor S, Tyagi AK, Khurana JP (2007) F-box proteins in rice. genome-wide analysis, classiﬁcation, temporal and spatial gene expression during panicle and seed development, and regulation by light and abiotic stress.Plant Physiol 143: 1467–1483.</ref>

<ref name="pmid: 19117763"> Zhang H, Tan GLL, Yang LNN, Yang JC, Zhang JH, Zhao BH (2009) Hormones in the grains and roots in relation to post-anthesis development of inferior and superior spikelets in japonica/indica hybrid rice. Plant Physiol Biochem 47: 195–204.</ref>

</references>

Please input cited references here.
{{JaponicaGene|
GeneName = Os12g0189500|
Description = Pyridine nucleotide-disulphide oxidoreductase, class I family protein|
Version = NM_001072867.2 GI:297612801 GeneID:4351695|
Length = 8650 bp|
Definition = Oryza sativa Japonica Group Os12g0189500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 12|Chromosome 12]]|
AP = Chromosome 12:4507750..4516399|
CDS = 4507750..4507887,4514763..4514909,4515209..4515331,4515422..4515658,4515788..4516399 |
GCID = <gbrowseImage1>
name=NC_008405:4507750..4516399
source=RiceChromosome12
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008405:4507750..4516399
source=RiceChromosome12
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggagaaggctctagttctgattgttggagcaggcccatctggtcttgcaacagcggcgtgccttggccagctctctataccctatgttattatcgagcgtgaggattgtaccgcgtcgctgtggcgtaagcacacatatgatcgcctcaagcttcacctcgcaaaggagttctgcgagatgccccacatgccatatccggaagacacaccaacctacatccccaagattcaatttttgaggtatatggatgattatgtggagcatttcaacatttgtcccaaatttaactcatctgttgagtcatgtttgtatgatgatgtgcaaaaatattgggttgtcacaacacatgaccaagtgaatggcatggtgtccaagtatgcagctaggttcttagttgtagcaagtggtgaaaatagtgcagggaacattcctagcatccctggactagaggatttctctggccatgtcatacactcatcaagctttaggtcagcagattcctatgctgcgcaaagggtgcttgttgttggatgtggaaactcaggcatggagattgcctatgatctttcaagccatggagcaaatacctccatagtcatccgcagccctctgcatgtgatgacaaaggagctgattcacatggggatgaaacttgcaagctggagcctcccagtgaaatttgttgattttattcttgtggtccttgcatatctctggtttggcaatctctcaaagtatggcatcgtgaggcccaataaggggccattgcttcttaaggcaaatactggtcggtcagcagtaatagatgttggcactgtggagttaataaagaaaggggatatcaaggtctttggtacaatatcttgcatcaagggaaatgttgttgaatttgatgatgggaaagaaagttactttgatgctattgtttttgctacaggctacacaagtactgcaaacaactggcttaagaatggtgaggatatgatgaacaaagaaggcatgcccaagaaggacttcccaaatcactggaaaggttcaaatggactctactgtgttgggtttgcgaggagaggattatctggtattgctcatgacgccaaaaacgttgctaatgactggatggaagaagacttgacaccaaagcccgccgttggcagttctaagaacagtcagccctcacttatcacatccatggatggtgaacgatgctttgccttagtggcagagcaaactgtttccattggaggcaattga</cdnaseq>|
AA = <aaseq>MEKALVLIVGAGPSGLATAACLGQLSIPYVIIEREDCTASLWRK HTYDRLKLHLAKEFCEMPHMPYPEDTPTYIPKIQFLRYMDDYVEHFNICPKFNSSVES CLYDDVQKYWVVTTHDQVNGMVSKYAARFLVVASGENSAGNIPSIPGLEDFSGHVIHS SSFRSADSYAAQRVLVVGCGNSGMEIAYDLSSHGANTSIVIRSPLHVMTKELIHMGMK LASWSLPVKFVDFILVVLAYLWFGNLSKYGIVRPNKGPLLLKANTGRSAVIDVGTVEL IKKGDIKVFGTISCIKGNVVEFDDGKESYFDAIVFATGYTSTANNWLKNGEDMMNKEG MPKKDFPNHWKGSNGLYCVGFARRGLSGIAHDAKNVANDWMEEDLTPKPAVGSSKNSQ PSLITSMDGERCFALVAEQTVSIGGN</aaseq>|
DNA = <dnaseqindica>8513..8650#1491..1637#1069..1191#742..978#1..612#atggagaaggctctagttctgattgttggagcaggcccatctggtcttgcaacagcggcgtgccttggccagctctctataccctatgttattatcgagcgtgaggattgtaccgcgtcgctgtggcgtaagcacacatatgatcgcctcaagcttcacctcgcaaaggagttctgcgagatgccccacatgccatatccggaagacacaccaacctacatccccaagattcaatttttgaggtatatggatgattatgtggagcatttcaacatttgtcccaaatttaactcatctgttgagtcatgtttgtatgatgatgtgcaaaaatattgggttgtcacaacacatgaccaagtgaatggcatggtgtccaagtatgcagctaggttcttagttgtagcaagtggtgaaaatagtgcagggaacattcctagcatccctggactagaggatttctctggccatgtcatacactcatcaagctttaggtcagcagattcctatgctgcgcaaagggtgcttgttgttggatgtggaaactcaggcatggagattgcctatgatctttcaagccatggagcaaatacctccatagtcatccgcagccctgtaagtaaataatacatttgcataaaagtttcctttctttattttgcctccctgttttccaggtcaagttattgtaagtatcctattaaatcattttcttacaccacaaattcttgaattattctaaagctgcatgtgatgacaaaggagctgattcacatggggatgaaacttgcaagctggagcctcccagtgaaatttgttgattttattcttgtggtccttgcatatctctggtttggcaatctctcaaagtatggcatcgtgaggcccaataaggggccattgcttcttaaggcaaatactggtcggtcagcagtaatagatgttggcactgtggagttaataaagaaaggggatatcaaggtaacaaaattcttgacacaacatagaaaagtctatataaattctatttggtacttgacaggttctataaggaatttatgtggcatgtaggtctttggtacaatatcttgcatcaagggaaatgttgttgaatttgatgatgggaaagaaagttactttgatgctattgtttttgctacaggctacacaagtactgcaaacaactggcttaaggtatgtaatatgttgctcatatggtaaatagtaaatacaaaaattattgcaattagatttagttgttattttctctctcctgcatacctcaagtggggcatcaaattaagagtgcctacaatactattagacagatttagaaagaaaagcatttgcgtgattgactcaacaaaattagtgataaaacctgttataaaattatagttataattagaagacaatggtgctatcttcatatttaactttctttctttctgtggaaatatttgttaataaaataattatgattaaaaacgcagaatggtgaggatatgatgaacaaagaaggcatgcccaagaaggacttcccaaatcactggaaaggttcaaatggactctactgtgttgggtttgcgaggagaggattatctggtattgctcatgacgccaaaaacgttgctaatgacgtcaaggccttcctagatttgatggcaccattctaattattatttgcactggaatgatgcctatcaaatagtattgtggtaccttctacaatagtggtgtgtacttaatgttacattatgtttgaagcaagctaaatgttatgcaatctccagagttgtttaaataattatattttcttaatcgacatgttggatgagcttttatttaaagctctttgctggaatacatgaatatgtggtttattctttataatgtgtgaagccctttgtttggatccatgaaaaataaaatcagaaactcatgtcaaacctcccctcacacctgtattacctatcctactctaaatcataccccctcctccccccatcggcaccatagttgttcagccagtgacgccctgcccttttgaaagaagttgagcatcactgccggttcatggctgtttaggcgggaaaattcactccaacgccatgaactggtaaagattggcaatccctgttggctcacaccgaaccgatagagatcagtgggcaaggattgccctttctgtaccagtaacacctttatccactccagttatgaaaaaaaaatgctctttagagttgtttgttccttatttcaactcaacacaacaatatggagttctaaaaaataacacaacattatggagaatactaaataatagtagagatcatagattacttagaagttagaacatacctaaaagggagattttttttttaaaaaaagaattcattttcaatcctatgccttattttcactgacatttggaaagggagtagtatgtctatttggggaagaggttcttttttttttttggggggcggggggggggggggggagggggaagggagggctgctttgattaattcctcccattgtattacatttcacttggatggatcatacctcctattcttctagataccacgaacaaatgtcattgaattatgagatagactaaaatctgcgggtgtcaaagatttcagaaaacttgagtctttgatgtaaatgttggtggaaattgcacccttttatatcaaatgcccattgggtttgtgcagctagcctaaaaaagattattccccattttggaaggttatgtgttacaaatcagacatctccgcaatattttcagaaaactaaagtgggtactaatactatatttttgcatgatcctggcctaaggtggtattacaatctgttgagatatgcaatggtcaaagagtggtactgtacattctattagagagctagattttattttgaactctagatgtgggtaactgatgatactggattgacgaaagccgaaagcccatctttatgagttttttaaggacttggtaggatcaatatataaacttttaataggagaaaatgtacatttactataaccaatttttacatgacgcctcgacaggaggcacccctcccctaggcatgcctctaaagttagccctgttagtgttctatcccttttgagcagaccattcttgagtaggttacaaatgtcacactgtttgatactccatttgtcctaaaatataaggtggccatatatagttttagcaaaaccaacatatgtaggttttgacaaataacaaactaacaatatactgtatatgtttaatgccatccatttcatatcctaagattcatatttgaaagcactcttgtatgatgtaattttctagttcttgataatatattattgaagaaactaattgttaaaatataatattgtagacagtgtaagaaaactaatatgctttatagtttgagactgataggactattataaaaaggtcctcctattacacttgatgagaatgatggtaaaattgcatgatatataatttgtgaaagctggcacatattcaatgaaatcgatatattctttggatgatcacgggtgtttctgggccaaatttctactttatttgtaatcccgagagcttcatctacaagtaaaaaaaaaatccaggtttttagttgataatgtagtctatcttaataaaggaagagatgcaaaggaggaaatttcctgcaaacaccggaatgtcttttcttccctgactgaaaatacagttttgttctttacatgtaaattagctagcaaagttatttaggggcattacaggtttagatatgctttggcaccaaaaccaggtacagaacaaatgttcgctggatgcactgcatcgatcttgatgtactccctatgttcaattatataagacatttttggtttgttctaatcaaacttgaatatacatattttcagtttgaccaaacaatgtagtagtgtttccaacacaaaacaaatatactacagaaatatatttaatggtggatttaatgaaaccaataaagagtatgcgataatagtaagtccatctaatgcataaaatcctggtgcattactatcctaggttaatatggcaccacctatactaagatcttcttctttctcttcatgtttgattgtacatcttgatatttccttccatgtgcatgcatggtttcaacaaaaaaaaaggtaacactgacttgttccaataatacacattgtagttatctatagcccttttttacatacatattacccccattatgtttgcagttgttacccccctttatctgattagagcataaaggacctgtggaatttttactcttataacccgactttttttatctattccataacctacaaatgggtttcctattttgatttgtaaaaataacaaagagaaagcctttgctctgatggtcacaatccttcgatttattccttttgtttgtaataatgttagtaaagtaatccatcagttgatttataaccccctctcgccaatgaaactgattcacttttatgaagtgaggagaaagaagggatcaattaatcgaggatccaatattttagtccctcgaggaagtctcctaaatcattggtagtattcatttgactctactagttacaactaataagacatatttataagaaagaaaatgcagcaggaggattactgatttcaattagttactatgtgcaaaattcagtgctcgcgtgccggggggggggggggaggggcggcaacggagttattgttcctacatacaataaatagtgatgacatgctctagtgaagttatttcagactggatccacatgggtgtctttcttttgtttctttggaaaggcttgttgtagctatctcatcttgataggtcgaaagattcgttcgttagtaacgacttgcttaaacaaagtt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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001072867.2 RefSeq:Os12g0189500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 12]]
[[Category:Chromosome 12]]

Os12g0189500

2014-06-03T14:14:15Z

Hylwayne: Undo revision 176936 by Hylwayne (talk)

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.
The increase in IAA content was strongly correlated with the expression of putative
IAA biosynthesis genes, OsYUC9, OsYUC11 and OsTAR1, measured by
quantitative reverse transcriptase polymerase chain reaction<ref name="pmid:16660061" />. These results
conﬁrm the importance of the tryptophan aminotransferase/YUCCA pathway
in this system. All three genes were expressed in endosperm; expression
of OsYUC11 appeared to be conﬁned to endosperm tissue<ref name="pmid:17889819" />. Phylogenetic
analysis indicated that OsYUC11 and AtYUC10 belong to a separate clade of
YUCCAs, which do not have orthologues outside the Angiosperms<ref name="pmid:18410479" />. This clade
may have evolved with a speciﬁc role in endosperm. Expression of tryptophan
decarboxylase in developing rice grains did not correlate with IAA levels,
indicating that tryptamine is unlikely to be important for IAA synthesis in this
system<ref name="pmid:16665995" />. In light of these observations, we hypothesize that IAA production
in developing rice grains is controlled via expression of OsTAR1, OsYUC9,
OsYUC11 and that IAA may be important during starch deposition in addition
to its pr eviously suggested role early in grain development.

===Expression===
Quantitative RT–PCR results for OsYUC3, OsYUC 9, OsYUC 11, OsYUC 12, OsYUC 14, OsTAR1, OsTAR2 and OsTDC1. Total RNA was extracted using a Qiagen RNeasy Kit. Reaction tubes were prepared using a CAS-1200 Robotic liquid-handling system, Qiagen. Each tube contained 250 ng
RNA per 25 μL reaction and a ﬁnal primer concentration of 0.5 μM. Ampliﬁcation was carried out using a Qiagen QuantiTect SYBR Green RT–PCR kit in a Corbett Research Rotor-Gene 3000 thermocycler ﬁtted with a 72-tube rotor for 45 cycles. Expression was calculated relative to reference genes UBC using ROTORGENE software (Qiagen) <ref name="pmid: 16690022 " />. Melt curve analysis conﬁrmed that a single product was obtained for each gene ampliﬁed. Results shown are the means ± the standard error of three biological replicates and two technical replicates. Comparison of the expression of YUCCA, TAA/TAR and TDC orthologues during grain/seed development <ref name="pmid: 17293439 " />.

===Evolution===
Please input evolution information here.

Phylogenetic tree of FMOs from rice (Os), Arabidopsis (At), Selaginella (Smoe), Physcomitrella (Ppls) and Chlamydomonas (Cr/Cre). Human FMOs, translated Pinus cDNAs and four sequences from bacteria, representing the most closely related proteins to YUCCA from outside the plant kingdom were also included. Multiple sequence alignments were carried out using MUSCLE (Edgar 2004); trees were constructed using PROTDIST and FITSCH bootstrap values for major branches are indicated. Scale bar indicates 0.1 amino acid substitutions persite<ref name="pmid: 19117763">. Examination of the expression of YUCCA in developing seeds using both RT–PCR and microarray data indicated that OsYUC11 and AtYUC10 appeared to be speciﬁc to, and highly expressed in, endosperm tissue. These two proteins are orthologues of each other and also of ZmYUC, which appears to be important for IAA synthesis in developing maize kernels.
You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

Present address: Department of applied biological science, Al-Ghad International AppliedMedical Science University, Jeddah, Saudi Arabia

==References==
<references>

<ref name="pmid:16660061">Bandurski RS, Schulze A (1977) Concentration of indole-3-acetic acid and its derivatives in plants. Plant Physiol 60: 211–213.</ref>
<ref name="pmid:17889819"> Barkawi LS, Tam YY, Tillman JA, Pederson B, Calio J,Al-Amier H, Emerick M, Normanly J, Cohen JD (2008) A high-throughput method for the quantitative analysis of indole-3-acetic acid and other auxins from plant tissue. Anal Biochem 372: 177–188 </ref>
<ref name="pmid:18410479 "> Benedito VA, Torres-Jerez I, Murray JD, Andriankaja A,Allen S, Kakar K, Wandrey M, Verdier J, Zuber H, Ott T,Moreau S, Niebel A, Frickey T, Weiller G, He J, Dai XB,Zhao PX, Tang YH, Udvardi MK (2008) A gene expression atlas of the model legume Medicago truncatula. Plant J 55: 504–513. </ref>
<ref name="pmid:16665995 ">Chen KH, Miller AN, Patterson GW, Cohen JD (1988) A Rapid and simple procedure for puriﬁcation of indole-3-acetic-acid prior to GC-SIM-MS analysis. Plant Physiol 86: 822–825 </ref>
<ref name="pmid: 16690022"> Jain M, Nijhawan A, Tyagi AK, Khurana JP (2006) Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Biochem Biophys Res Commun 345: 646–651.</ref>

<ref name="pmid: 17293439"> Jain M, Nijhawan A, Arora R, Agarwal P, Ray S, Sharma P, Kapoor S, Tyagi AK, Khurana JP (2007) F-box proteins in rice. genome-wide analysis, classiﬁcation, temporal and spatial gene expression during panicle and seed development, and regulation by light and abiotic stress.Plant Physiol 143: 1467–1483.</ref>

<ref name="pmid: 19117763"> Zhang H, Tan GLL, Yang LNN, Yang JC, Zhang JH, Zhao BH (2009) Hormones in the grains and roots in relation to post-anthesis development of inferior and superior spikelets in japonica/indica hybrid rice. Plant Physiol Biochem 47: 195–204.</ref>

Please input cited references here.
{{JaponicaGene|
GeneName = Os12g0189500|
Description = Pyridine nucleotide-disulphide oxidoreductase, class I family protein|
Version = NM_001072867.2 GI:297612801 GeneID:4351695|
Length = 8650 bp|
Definition = Oryza sativa Japonica Group Os12g0189500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 12|Chromosome 12]]|
AP = Chromosome 12:4507750..4516399|
CDS = 4507750..4507887,4514763..4514909,4515209..4515331,4515422..4515658,4515788..4516399 |
GCID = <gbrowseImage1>
name=NC_008405:4507750..4516399
source=RiceChromosome12
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008405:4507750..4516399
source=RiceChromosome12
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggagaaggctctagttctgattgttggagcaggcccatctggtcttgcaacagcggcgtgccttggccagctctctataccctatgttattatcgagcgtgaggattgtaccgcgtcgctgtggcgtaagcacacatatgatcgcctcaagcttcacctcgcaaaggagttctgcgagatgccccacatgccatatccggaagacacaccaacctacatccccaagattcaatttttgaggtatatggatgattatgtggagcatttcaacatttgtcccaaatttaactcatctgttgagtcatgtttgtatgatgatgtgcaaaaatattgggttgtcacaacacatgaccaagtgaatggcatggtgtccaagtatgcagctaggttcttagttgtagcaagtggtgaaaatagtgcagggaacattcctagcatccctggactagaggatttctctggccatgtcatacactcatcaagctttaggtcagcagattcctatgctgcgcaaagggtgcttgttgttggatgtggaaactcaggcatggagattgcctatgatctttcaagccatggagcaaatacctccatagtcatccgcagccctctgcatgtgatgacaaaggagctgattcacatggggatgaaacttgcaagctggagcctcccagtgaaatttgttgattttattcttgtggtccttgcatatctctggtttggcaatctctcaaagtatggcatcgtgaggcccaataaggggccattgcttcttaaggcaaatactggtcggtcagcagtaatagatgttggcactgtggagttaataaagaaaggggatatcaaggtctttggtacaatatcttgcatcaagggaaatgttgttgaatttgatgatgggaaagaaagttactttgatgctattgtttttgctacaggctacacaagtactgcaaacaactggcttaagaatggtgaggatatgatgaacaaagaaggcatgcccaagaaggacttcccaaatcactggaaaggttcaaatggactctactgtgttgggtttgcgaggagaggattatctggtattgctcatgacgccaaaaacgttgctaatgactggatggaagaagacttgacaccaaagcccgccgttggcagttctaagaacagtcagccctcacttatcacatccatggatggtgaacgatgctttgccttagtggcagagcaaactgtttccattggaggcaattga</cdnaseq>|
AA = <aaseq>MEKALVLIVGAGPSGLATAACLGQLSIPYVIIEREDCTASLWRK HTYDRLKLHLAKEFCEMPHMPYPEDTPTYIPKIQFLRYMDDYVEHFNICPKFNSSVES CLYDDVQKYWVVTTHDQVNGMVSKYAARFLVVASGENSAGNIPSIPGLEDFSGHVIHS SSFRSADSYAAQRVLVVGCGNSGMEIAYDLSSHGANTSIVIRSPLHVMTKELIHMGMK LASWSLPVKFVDFILVVLAYLWFGNLSKYGIVRPNKGPLLLKANTGRSAVIDVGTVEL IKKGDIKVFGTISCIKGNVVEFDDGKESYFDAIVFATGYTSTANNWLKNGEDMMNKEG MPKKDFPNHWKGSNGLYCVGFARRGLSGIAHDAKNVANDWMEEDLTPKPAVGSSKNSQ PSLITSMDGERCFALVAEQTVSIGGN</aaseq>|
DNA = <dnaseqindica>8513..8650#1491..1637#1069..1191#742..978#1..612#atggagaaggctctagttctgattgttggagcaggcccatctggtcttgcaacagcggcgtgccttggccagctctctataccctatgttattatcgagcgtgaggattgtaccgcgtcgctgtggcgtaagcacacatatgatcgcctcaagcttcacctcgcaaaggagttctgcgagatgccccacatgccatatccggaagacacaccaacctacatccccaagattcaatttttgaggtatatggatgattatgtggagcatttcaacatttgtcccaaatttaactcatctgttgagtcatgtttgtatgatgatgtgcaaaaatattgggttgtcacaacacatgaccaagtgaatggcatggtgtccaagtatgcagctaggttcttagttgtagcaagtggtgaaaatagtgcagggaacattcctagcatccctggactagaggatttctctggccatgtcatacactcatcaagctttaggtcagcagattcctatgctgcgcaaagggtgcttgttgttggatgtggaaactcaggcatggagattgcctatgatctttcaagccatggagcaaatacctccatagtcatccgcagccctgtaagtaaataatacatttgcataaaagtttcctttctttattttgcctccctgttttccaggtcaagttattgtaagtatcctattaaatcattttcttacaccacaaattcttgaattattctaaagctgcatgtgatgacaaaggagctgattcacatggggatgaaacttgcaagctggagcctcccagtgaaatttgttgattttattcttgtggtccttgcatatctctggtttggcaatctctcaaagtatggcatcgtgaggcccaataaggggccattgcttcttaaggcaaatactggtcggtcagcagtaatagatgttggcactgtggagttaataaagaaaggggatatcaaggtaacaaaattcttgacacaacatagaaaagtctatataaattctatttggtacttgacaggttctataaggaatttatgtggcatgtaggtctttggtacaatatcttgcatcaagggaaatgttgttgaatttgatgatgggaaagaaagttactttgatgctattgtttttgctacaggctacacaagtactgcaaacaactggcttaaggtatgtaatatgttgctcatatggtaaatagtaaatacaaaaattattgcaattagatttagttgttattttctctctcctgcatacctcaagtggggcatcaaattaagagtgcctacaatactattagacagatttagaaagaaaagcatttgcgtgattgactcaacaaaattagtgataaaacctgttataaaattatagttataattagaagacaatggtgctatcttcatatttaactttctttctttctgtggaaatatttgttaataaaataattatgattaaaaacgcagaatggtgaggatatgatgaacaaagaaggcatgcccaagaaggacttcccaaatcactggaaaggttcaaatggactctactgtgttgggtttgcgaggagaggattatctggtattgctcatgacgccaaaaacgttgctaatgacgtcaaggccttcctagatttgatggcaccattctaattattatttgcactggaatgatgcctatcaaatagtattgtggtaccttctacaatagtggtgtgtacttaatgttacattatgtttgaagcaagctaaatgttatgcaatctccagagttgtttaaataattatattttcttaatcgacatgttggatgagcttttatttaaagctctttgctggaatacatgaatatgtggtttattctttataatgtgtgaagccctttgtttggatccatgaaaaataaaatcagaaactcatgtcaaacctcccctcacacctgtattacctatcctactctaaatcataccccctcctccccccatcggcaccatagttgttcagccagtgacgccctgcccttttgaaagaagttgagcatcactgccggttcatggctgtttaggcgggaaaattcactccaacgccatgaactggtaaagattggcaatccctgttggctcacaccgaaccgatagagatcagtgggcaaggattgccctttctgtaccagtaacacctttatccactccagttatgaaaaaaaaatgctctttagagttgtttgttccttatttcaactcaacacaacaatatggagttctaaaaaataacacaacattatggagaatactaaataatagtagagatcatagattacttagaagttagaacatacctaaaagggagattttttttttaaaaaaagaattcattttcaatcctatgccttattttcactgacatttggaaagggagtagtatgtctatttggggaagaggttcttttttttttttggggggcggggggggggggggggagggggaagggagggctgctttgattaattcctcccattgtattacatttcacttggatggatcatacctcctattcttctagataccacgaacaaatgtcattgaattatgagatagactaaaatctgcgggtgtcaaagatttcagaaaacttgagtctttgatgtaaatgttggtggaaattgcacccttttatatcaaatgcccattgggtttgtgcagctagcctaaaaaagattattccccattttggaaggttatgtgttacaaatcagacatctccgcaatattttcagaaaactaaagtgggtactaatactatatttttgcatgatcctggcctaaggtggtattacaatctgttgagatatgcaatggtcaaagagtggtactgtacattctattagagagctagattttattttgaactctagatgtgggtaactgatgatactggattgacgaaagccgaaagcccatctttatgagttttttaaggacttggtaggatcaatatataaacttttaataggagaaaatgtacatttactataaccaatttttacatgacgcctcgacaggaggcacccctcccctaggcatgcctctaaagttagccctgttagtgttctatcccttttgagcagaccattcttgagtaggttacaaatgtcacactgtttgatactccatttgtcctaaaatataaggtggccatatatagttttagcaaaaccaacatatgtaggttttgacaaataacaaactaacaatatactgtatatgtttaatgccatccatttcatatcctaagattcatatttgaaagcactcttgtatgatgtaattttctagttcttgataatatattattgaagaaactaattgttaaaatataatattgtagacagtgtaagaaaactaatatgctttatagtttgagactgataggactattataaaaaggtcctcctattacacttgatgagaatgatggtaaaattgcatgatatataatttgtgaaagctggcacatattcaatgaaatcgatatattctttggatgatcacgggtgtttctgggccaaatttctactttatttgtaatcccgagagcttcatctacaagtaaaaaaaaaatccaggtttttagttgataatgtagtctatcttaataaaggaagagatgcaaaggaggaaatttcctgcaaacaccggaatgtcttttcttccctgactgaaaatacagttttgttctttacatgtaaattagctagcaaagttatttaggggcattacaggtttagatatgctttggcaccaaaaccaggtacagaacaaatgttcgctggatgcactgcatcgatcttgatgtactccctatgttcaattatataagacatttttggtttgttctaatcaaacttgaatatacatattttcagtttgaccaaacaatgtagtagtgtttccaacacaaaacaaatatactacagaaatatatttaatggtggatttaatgaaaccaataaagagtatgcgataatagtaagtccatctaatgcataaaatcctggtgcattactatcctaggttaatatggcaccacctatactaagatcttcttctttctcttcatgtttgattgtacatcttgatatttccttccatgtgcatgcatggtttcaacaaaaaaaaaggtaacactgacttgttccaataatacacattgtagttatctatagcccttttttacatacatattacccccattatgtttgcagttgttacccccctttatctgattagagcataaaggacctgtggaatttttactcttataacccgactttttttatctattccataacctacaaatgggtttcctattttgatttgtaaaaataacaaagagaaagcctttgctctgatggtcacaatccttcgatttattccttttgtttgtaataatgttagtaaagtaatccatcagttgatttataaccccctctcgccaatgaaactgattcacttttatgaagtgaggagaaagaagggatcaattaatcgaggatccaatattttagtccctcgaggaagtctcctaaatcattggtagtattcatttgactctactagttacaactaataagacatatttataagaaagaaaatgcagcaggaggattactgatttcaattagttactatgtgcaaaattcagtgctcgcgtgccggggggggggggggaggggcggcaacggagttattgttcctacatacaataaatagtgatgacatgctctagtgaagttatttcagactggatccacatgggtgtctttcttttgtttctttggaaaggcttgttgtagctatctcatcttgataggtcgaaagattcgttcgttagtaacgacttgcttaaacaaagtt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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001072867.2 RefSeq:Os12g0189500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 12]]
[[Category:Chromosome 12]]

Os12g0189500

2014-06-03T14:12:35Z

Hylwayne:

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.
The increase in IAA content was strongly correlated with the expression of putative
IAA biosynthesis genes, OsYUC9, OsYUC11 and OsTAR1, measured by
quantitative reverse transcriptase polymerase chain reaction<ref name="pmid:16660061" />. These results
conﬁrm the importance of the tryptophan aminotransferase/YUCCA pathway
in this system. All three genes were expressed in endosperm; expression
of OsYUC11 appeared to be conﬁned to endosperm tissue<ref name="pmid:17889819" />. Phylogenetic
analysis indicated that OsYUC11 and AtYUC10 belong to a separate clade of
YUCCAs, which do not have orthologues outside the Angiosperms<ref name="pmid:18410479" />. This clade
may have evolved with a speciﬁc role in endosperm. Expression of tryptophan
decarboxylase in developing rice grains did not correlate with IAA levels,
indicating that tryptamine is unlikely to be important for IAA synthesis in this
system<ref name="pmid:16665995" />. In light of these observations, we hypothesize that IAA production
in developing rice grains is controlled via expression of OsTAR1, OsYUC9,
OsYUC11 and that IAA may be important during starch deposition in addition
to its pr eviously suggested role early in grain development.

===Expression===
Quantitative RT–PCR results for OsYUC3, OsYUC 9, OsYUC 11, OsYUC 12, OsYUC 14, OsTAR1, OsTAR2 and OsTDC1. Total RNA was extracted using a Qiagen RNeasy Kit. Reaction tubes were prepared using a CAS-1200 Robotic liquid-handling system, Qiagen. Each tube contained 250 ng
RNA per 25 μL reaction and a ﬁnal primer concentration of 0.5 μM. Ampliﬁcation was carried out using a Qiagen QuantiTect SYBR Green RT–PCR kit in a Corbett Research Rotor-Gene 3000 thermocycler ﬁtted with a 72-tube rotor for 45 cycles. Expression was calculated relative to reference genes UBC using ROTORGENE software (Qiagen) <ref name="pmid: 16690022 " />. Melt curve analysis conﬁrmed that a single product was obtained for each gene ampliﬁed. Results shown are the means ± the standard error of three biological replicates and two technical replicates. Comparison of the expression of YUCCA, TAA/TAR and TDC orthologues during grain/seed development <ref name="pmid: 17293439 " />.

===Evolution===
Please input evolution information here.

Phylogenetic tree of FMOs from rice (Os), Arabidopsis (At), Selaginella (Smoe), Physcomitrella (Ppls) and Chlamydomonas (Cr/Cre). Human FMOs, translated Pinus cDNAs and four sequences from bacteria, representing the most closely related proteins to YUCCA from outside the plant kingdom were also included. Multiple sequence alignments were carried out using MUSCLE (Edgar 2004); trees were constructed using PROTDIST and FITSCH bootstrap values for major branches are indicated. Scale bar indicates 0.1 amino acid substitutions persite<ref name="pmid: 19117763">. Examination of the expression of YUCCA in developing seeds using both RT–PCR and microarray data indicated that OsYUC11 and AtYUC10 appeared to be speciﬁc to, and highly expressed in, endosperm tissue. These two proteins are orthologues of each other and also of ZmYUC, which appears to be important for IAA synthesis in developing maize kernels.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

Present address: Department of applied biological science, Al-Ghad International AppliedMedical Science University, Jeddah, Saudi Arabia

==References==
<references>

<ref name="pmid:16660061">Bandurski RS, Schulze A (1977) Concentration of indole-3-acetic acid and its derivatives in plants. Plant Physiol 60: 211–213.</ref>
<ref name="pmid:17889819"> Barkawi LS, Tam YY, Tillman JA, Pederson B, Calio J,Al-Amier H, Emerick M, Normanly J, Cohen JD (2008) A high-throughput method for the quantitative analysis of indole-3-acetic acid and other auxins from plant tissue. Anal Biochem 372: 177–188 </ref>
<ref name="pmid:18410479 "> Benedito VA, Torres-Jerez I, Murray JD, Andriankaja A,Allen S, Kakar K, Wandrey M, Verdier J, Zuber H, Ott T,Moreau S, Niebel A, Frickey T, Weiller G, He J, Dai XB,Zhao PX, Tang YH, Udvardi MK (2008) A gene expression atlas of the model legume Medicago truncatula. Plant J 55: 504–513. </ref>
<ref name="pmid:16665995 ">Chen KH, Miller AN, Patterson GW, Cohen JD (1988) A Rapid and simple procedure for puriﬁcation of indole-3-acetic-acid prior to GC-SIM-MS analysis. Plant Physiol 86: 822–825 </ref>
<ref name="pmid: 16690022"> Jain M, Nijhawan A, Tyagi AK, Khurana JP (2006) Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Biochem Biophys Res Commun 345: 646–651.</ref>

<ref name="pmid: 17293439"> Jain M, Nijhawan A, Arora R, Agarwal P, Ray S, Sharma P, Kapoor S, Tyagi AK, Khurana JP (2007) F-box proteins in rice. genome-wide analysis, classiﬁcation, temporal and spatial gene expression during panicle and seed development, and regulation by light and abiotic stress.Plant Physiol 143: 1467–1483.</ref>
<ref name="pmid: 19117763"> Zhang H, Tan GLL, Yang LNN, Yang JC, Zhang JH, Zhao BH (2009) Hormones in the grains and roots in relation to post-anthesis development of inferior and superior spikelets in japonica/indica hybrid rice. Plant Physiol Biochem 47: 195–204.</ref>

Please input cited references here.
{{JaponicaGene|
GeneName = Os12g0189500|
Description = Pyridine nucleotide-disulphide oxidoreductase, class I family protein|
Version = NM_001072867.2 GI:297612801 GeneID:4351695|
Length = 8650 bp|
Definition = Oryza sativa Japonica Group Os12g0189500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 12|Chromosome 12]]|
AP = Chromosome 12:4507750..4516399|
CDS = 4507750..4507887,4514763..4514909,4515209..4515331,4515422..4515658,4515788..4516399 |
GCID = <gbrowseImage1>
name=NC_008405:4507750..4516399
source=RiceChromosome12
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008405:4507750..4516399
source=RiceChromosome12
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggagaaggctctagttctgattgttggagcaggcccatctggtcttgcaacagcggcgtgccttggccagctctctataccctatgttattatcgagcgtgaggattgtaccgcgtcgctgtggcgtaagcacacatatgatcgcctcaagcttcacctcgcaaaggagttctgcgagatgccccacatgccatatccggaagacacaccaacctacatccccaagattcaatttttgaggtatatggatgattatgtggagcatttcaacatttgtcccaaatttaactcatctgttgagtcatgtttgtatgatgatgtgcaaaaatattgggttgtcacaacacatgaccaagtgaatggcatggtgtccaagtatgcagctaggttcttagttgtagcaagtggtgaaaatagtgcagggaacattcctagcatccctggactagaggatttctctggccatgtcatacactcatcaagctttaggtcagcagattcctatgctgcgcaaagggtgcttgttgttggatgtggaaactcaggcatggagattgcctatgatctttcaagccatggagcaaatacctccatagtcatccgcagccctctgcatgtgatgacaaaggagctgattcacatggggatgaaacttgcaagctggagcctcccagtgaaatttgttgattttattcttgtggtccttgcatatctctggtttggcaatctctcaaagtatggcatcgtgaggcccaataaggggccattgcttcttaaggcaaatactggtcggtcagcagtaatagatgttggcactgtggagttaataaagaaaggggatatcaaggtctttggtacaatatcttgcatcaagggaaatgttgttgaatttgatgatgggaaagaaagttactttgatgctattgtttttgctacaggctacacaagtactgcaaacaactggcttaagaatggtgaggatatgatgaacaaagaaggcatgcccaagaaggacttcccaaatcactggaaaggttcaaatggactctactgtgttgggtttgcgaggagaggattatctggtattgctcatgacgccaaaaacgttgctaatgactggatggaagaagacttgacaccaaagcccgccgttggcagttctaagaacagtcagccctcacttatcacatccatggatggtgaacgatgctttgccttagtggcagagcaaactgtttccattggaggcaattga</cdnaseq>|
AA = <aaseq>MEKALVLIVGAGPSGLATAACLGQLSIPYVIIEREDCTASLWRK HTYDRLKLHLAKEFCEMPHMPYPEDTPTYIPKIQFLRYMDDYVEHFNICPKFNSSVES CLYDDVQKYWVVTTHDQVNGMVSKYAARFLVVASGENSAGNIPSIPGLEDFSGHVIHS SSFRSADSYAAQRVLVVGCGNSGMEIAYDLSSHGANTSIVIRSPLHVMTKELIHMGMK LASWSLPVKFVDFILVVLAYLWFGNLSKYGIVRPNKGPLLLKANTGRSAVIDVGTVEL IKKGDIKVFGTISCIKGNVVEFDDGKESYFDAIVFATGYTSTANNWLKNGEDMMNKEG MPKKDFPNHWKGSNGLYCVGFARRGLSGIAHDAKNVANDWMEEDLTPKPAVGSSKNSQ PSLITSMDGERCFALVAEQTVSIGGN</aaseq>|
DNA = <dnaseqindica>8513..8650#1491..1637#1069..1191#742..978#1..612#atggagaaggctctagttctgattgttggagcaggcccatctggtcttgcaacagcggcgtgccttggccagctctctataccctatgttattatcgagcgtgaggattgtaccgcgtcgctgtggcgtaagcacacatatgatcgcctcaagcttcacctcgcaaaggagttctgcgagatgccccacatgccatatccggaagacacaccaacctacatccccaagattcaatttttgaggtatatggatgattatgtggagcatttcaacatttgtcccaaatttaactcatctgttgagtcatgtttgtatgatgatgtgcaaaaatattgggttgtcacaacacatgaccaagtgaatggcatggtgtccaagtatgcagctaggttcttagttgtagcaagtggtgaaaatagtgcagggaacattcctagcatccctggactagaggatttctctggccatgtcatacactcatcaagctttaggtcagcagattcctatgctgcgcaaagggtgcttgttgttggatgtggaaactcaggcatggagattgcctatgatctttcaagccatggagcaaatacctccatagtcatccgcagccctgtaagtaaataatacatttgcataaaagtttcctttctttattttgcctccctgttttccaggtcaagttattgtaagtatcctattaaatcattttcttacaccacaaattcttgaattattctaaagctgcatgtgatgacaaaggagctgattcacatggggatgaaacttgcaagctggagcctcccagtgaaatttgttgattttattcttgtggtccttgcatatctctggtttggcaatctctcaaagtatggcatcgtgaggcccaataaggggccattgcttcttaaggcaaatactggtcggtcagcagtaatagatgttggcactgtggagttaataaagaaaggggatatcaaggtaacaaaattcttgacacaacatagaaaagtctatataaattctatttggtacttgacaggttctataaggaatttatgtggcatgtaggtctttggtacaatatcttgcatcaagggaaatgttgttgaatttgatgatgggaaagaaagttactttgatgctattgtttttgctacaggctacacaagtactgcaaacaactggcttaaggtatgtaatatgttgctcatatggtaaatagtaaatacaaaaattattgcaattagatttagttgttattttctctctcctgcatacctcaagtggggcatcaaattaagagtgcctacaatactattagacagatttagaaagaaaagcatttgcgtgattgactcaacaaaattagtgataaaacctgttataaaattatagttataattagaagacaatggtgctatcttcatatttaactttctttctttctgtggaaatatttgttaataaaataattatgattaaaaacgcagaatggtgaggatatgatgaacaaagaaggcatgcccaagaaggacttcccaaatcactggaaaggttcaaatggactctactgtgttgggtttgcgaggagaggattatctggtattgctcatgacgccaaaaacgttgctaatgacgtcaaggccttcctagatttgatggcaccattctaattattatttgcactggaatgatgcctatcaaatagtattgtggtaccttctacaatagtggtgtgtacttaatgttacattatgtttgaagcaagctaaatgttatgcaatctccagagttgtttaaataattatattttcttaatcgacatgttggatgagcttttatttaaagctctttgctggaatacatgaatatgtggtttattctttataatgtgtgaagccctttgtttggatccatgaaaaataaaatcagaaactcatgtcaaacctcccctcacacctgtattacctatcctactctaaatcataccccctcctccccccatcggcaccatagttgttcagccagtgacgccctgcccttttgaaagaagttgagcatcactgccggttcatggctgtttaggcgggaaaattcactccaacgccatgaactggtaaagattggcaatccctgttggctcacaccgaaccgatagagatcagtgggcaaggattgccctttctgtaccagtaacacctttatccactccagttatgaaaaaaaaatgctctttagagttgtttgttccttatttcaactcaacacaacaatatggagttctaaaaaataacacaacattatggagaatactaaataatagtagagatcatagattacttagaagttagaacatacctaaaagggagattttttttttaaaaaaagaattcattttcaatcctatgccttattttcactgacatttggaaagggagtagtatgtctatttggggaagaggttcttttttttttttggggggcggggggggggggggggagggggaagggagggctgctttgattaattcctcccattgtattacatttcacttggatggatcatacctcctattcttctagataccacgaacaaatgtcattgaattatgagatagactaaaatctgcgggtgtcaaagatttcagaaaacttgagtctttgatgtaaatgttggtggaaattgcacccttttatatcaaatgcccattgggtttgtgcagctagcctaaaaaagattattccccattttggaaggttatgtgttacaaatcagacatctccgcaatattttcagaaaactaaagtgggtactaatactatatttttgcatgatcctggcctaaggtggtattacaatctgttgagatatgcaatggtcaaagagtggtactgtacattctattagagagctagattttattttgaactctagatgtgggtaactgatgatactggattgacgaaagccgaaagcccatctttatgagttttttaaggacttggtaggatcaatatataaacttttaataggagaaaatgtacatttactataaccaatttttacatgacgcctcgacaggaggcacccctcccctaggcatgcctctaaagttagccctgttagtgttctatcccttttgagcagaccattcttgagtaggttacaaatgtcacactgtttgatactccatttgtcctaaaatataaggtggccatatatagttttagcaaaaccaacatatgtaggttttgacaaataacaaactaacaatatactgtatatgtttaatgccatccatttcatatcctaagattcatatttgaaagcactcttgtatgatgtaattttctagttcttgataatatattattgaagaaactaattgttaaaatataatattgtagacagtgtaagaaaactaatatgctttatagtttgagactgataggactattataaaaaggtcctcctattacacttgatgagaatgatggtaaaattgcatgatatataatttgtgaaagctggcacatattcaatgaaatcgatatattctttggatgatcacgggtgtttctgggccaaatttctactttatttgtaatcccgagagcttcatctacaagtaaaaaaaaaatccaggtttttagttgataatgtagtctatcttaataaaggaagagatgcaaaggaggaaatttcctgcaaacaccggaatgtcttttcttccctgactgaaaatacagttttgttctttacatgtaaattagctagcaaagttatttaggggcattacaggtttagatatgctttggcaccaaaaccaggtacagaacaaatgttcgctggatgcactgcatcgatcttgatgtactccctatgttcaattatataagacatttttggtttgttctaatcaaacttgaatatacatattttcagtttgaccaaacaatgtagtagtgtttccaacacaaaacaaatatactacagaaatatatttaatggtggatttaatgaaaccaataaagagtatgcgataatagtaagtccatctaatgcataaaatcctggtgcattactatcctaggttaatatggcaccacctatactaagatcttcttctttctcttcatgtttgattgtacatcttgatatttccttccatgtgcatgcatggtttcaacaaaaaaaaaggtaacactgacttgttccaataatacacattgtagttatctatagcccttttttacatacatattacccccattatgtttgcagttgttacccccctttatctgattagagcataaaggacctgtggaatttttactcttataacccgactttttttatctattccataacctacaaatgggtttcctattttgatttgtaaaaataacaaagagaaagcctttgctctgatggtcacaatccttcgatttattccttttgtttgtaataatgttagtaaagtaatccatcagttgatttataaccccctctcgccaatgaaactgattcacttttatgaagtgaggagaaagaagggatcaattaatcgaggatccaatattttagtccctcgaggaagtctcctaaatcattggtagtattcatttgactctactagttacaactaataagacatatttataagaaagaaaatgcagcaggaggattactgatttcaattagttactatgtgcaaaattcagtgctcgcgtgccggggggggggggggaggggcggcaacggagttattgttcctacatacaataaatagtgatgacatgctctagtgaagttatttcagactggatccacatgggtgtctttcttttgtttctttggaaaggcttgttgtagctatctcatcttgataggtcgaaagattcgttcgttagtaacgacttgcttaaacaaagtt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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001072867.2 RefSeq:Os12g0189500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 12]]
[[Category:Chromosome 12]]

Os12g0189500

2014-06-03T14:11:13Z

Hylwayne: /* Evolution */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.
The increase in IAA content was strongly correlated with the expression of putative
IAA biosynthesis genes, OsYUC9, OsYUC11 and OsTAR1, measured by
quantitative reverse transcriptase polymerase chain reaction<ref name="pmid:16660061" />. These results
conﬁrm the importance of the tryptophan aminotransferase/YUCCA pathway
in this system. All three genes were expressed in endosperm; expression
of OsYUC11 appeared to be conﬁned to endosperm tissue<ref name="pmid:17889819" />. Phylogenetic
analysis indicated that OsYUC11 and AtYUC10 belong to a separate clade of
YUCCAs, which do not have orthologues outside the Angiosperms<ref name="pmid:18410479" />. This clade
may have evolved with a speciﬁc role in endosperm. Expression of tryptophan
decarboxylase in developing rice grains did not correlate with IAA levels,
indicating that tryptamine is unlikely to be important for IAA synthesis in this
system<ref name="pmid:16665995" />. In light of these observations, we hypothesize that IAA production
in developing rice grains is controlled via expression of OsTAR1, OsYUC9,
OsYUC11 and that IAA may be important during starch deposition in addition
to its pr eviously suggested role early in grain development.

===Expression===
Quantitative RT–PCR results for OsYUC3, OsYUC 9, OsYUC 11, OsYUC 12, OsYUC 14, OsTAR1, OsTAR2 and OsTDC1. Total RNA was extracted using a Qiagen RNeasy Kit. Reaction tubes were prepared using a CAS-1200 Robotic liquid-handling system, Qiagen. Each tube contained 250 ng
RNA per 25 μL reaction and a ﬁnal primer concentration of 0.5 μM. Ampliﬁcation was carried out using a Qiagen QuantiTect SYBR Green RT–PCR kit in a Corbett Research Rotor-Gene 3000 thermocycler ﬁtted with a 72-tube rotor for 45 cycles. Expression was calculated relative to reference genes UBC using ROTORGENE software (Qiagen) <ref name="pmid: 16690022 " />. Melt curve analysis conﬁrmed that a single product was obtained for each gene ampliﬁed. Results shown are the means ± the standard error of three biological replicates and two technical replicates. Comparison of the expression of YUCCA, TAA/TAR and TDC orthologues during grain/seed development <ref name="pmid: 17293439 " />.

===Evolution===
Please input evolution information here.

Phylogenetic tree of FMOs from rice (Os), Arabidopsis (At), Selaginella (Smoe), Physcomitrella (Ppls) and Chlamydomonas (Cr/Cre). Human FMOs, translated Pinus cDNAs and four sequences from bacteria, representing the most closely related proteins to YUCCA from outside the plant kingdom were also included. Multiple sequence alignments were carried out using MUSCLE (Edgar 2004); trees were constructed using PROTDIST and FITSCH bootstrap values for major branches are indicated. Scale bar indicates 0.1 amino acid substitutions persite<ref name="pmid: 19117763">. Examination of the expression of YUCCA in developing seeds using both RT–PCR and microarray data indicated that OsYUC11 and AtYUC10 appeared to be speciﬁc to, and highly expressed in, endosperm tissue. These two proteins are orthologues of each other and also of ZmYUC, which appears to be important for IAA synthesis in developing maize kernels.

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==Labs working on this gene==
Please input related labs here.

Present address: Department of applied biological science, Al-Ghad International AppliedMedical Science University, Jeddah, Saudi Arabia

==References==
<references>

<ref name="pmid:16660061">Bandurski RS, Schulze A (1977) Concentration of indole-3-acetic acid and its derivatives in plants. Plant Physiol 60: 211–213.</ref>
<ref name="pmid:17889819"> Barkawi LS, Tam YY, Tillman JA, Pederson B, Calio J,Al-Amier H, Emerick M, Normanly J, Cohen JD (2008) A high-throughput method for the quantitative analysis of indole-3-acetic acid and other auxins from plant tissue. Anal Biochem 372: 177–188 </ref>
<ref name="pmid:18410479 "> Benedito VA, Torres-Jerez I, Murray JD, Andriankaja A,Allen S, Kakar K, Wandrey M, Verdier J, Zuber H, Ott T,Moreau S, Niebel A, Frickey T, Weiller G, He J, Dai XB,Zhao PX, Tang YH, Udvardi MK (2008) A gene expression atlas of the model legume Medicago truncatula. Plant J 55: 504–513. </ref>
<ref name="pmid:16665995 ">Chen KH, Miller AN, Patterson GW, Cohen JD (1988) A Rapid and simple procedure for puriﬁcation of indole-3-acetic-acid prior to GC-SIM-MS analysis. Plant Physiol 86: 822–825 </ref>
<ref name="pmid: 16690022"> Jain M, Nijhawan A, Tyagi AK, Khurana JP (2006) Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Biochem Biophys Res Commun 345: 646–651.</ref>

<ref name="pmid: 17293439"> Jain M, Nijhawan A, Arora R, Agarwal P, Ray S, Sharma P, Kapoor S, Tyagi AK, Khurana JP (2007) F-box proteins in rice. genome-wide analysis, classiﬁcation, temporal and spatial gene expression during panicle and seed development, and regulation by light and abiotic stress.Plant Physiol 143: 1467–1483.</ref>

Please input cited references here.
{{JaponicaGene|
GeneName = Os12g0189500|
Description = Pyridine nucleotide-disulphide oxidoreductase, class I family protein|
Version = NM_001072867.2 GI:297612801 GeneID:4351695|
Length = 8650 bp|
Definition = Oryza sativa Japonica Group Os12g0189500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 12|Chromosome 12]]|
AP = Chromosome 12:4507750..4516399|
CDS = 4507750..4507887,4514763..4514909,4515209..4515331,4515422..4515658,4515788..4516399 |
GCID = <gbrowseImage1>
name=NC_008405:4507750..4516399
source=RiceChromosome12
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008405:4507750..4516399
source=RiceChromosome12
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggagaaggctctagttctgattgttggagcaggcccatctggtcttgcaacagcggcgtgccttggccagctctctataccctatgttattatcgagcgtgaggattgtaccgcgtcgctgtggcgtaagcacacatatgatcgcctcaagcttcacctcgcaaaggagttctgcgagatgccccacatgccatatccggaagacacaccaacctacatccccaagattcaatttttgaggtatatggatgattatgtggagcatttcaacatttgtcccaaatttaactcatctgttgagtcatgtttgtatgatgatgtgcaaaaatattgggttgtcacaacacatgaccaagtgaatggcatggtgtccaagtatgcagctaggttcttagttgtagcaagtggtgaaaatagtgcagggaacattcctagcatccctggactagaggatttctctggccatgtcatacactcatcaagctttaggtcagcagattcctatgctgcgcaaagggtgcttgttgttggatgtggaaactcaggcatggagattgcctatgatctttcaagccatggagcaaatacctccatagtcatccgcagccctctgcatgtgatgacaaaggagctgattcacatggggatgaaacttgcaagctggagcctcccagtgaaatttgttgattttattcttgtggtccttgcatatctctggtttggcaatctctcaaagtatggcatcgtgaggcccaataaggggccattgcttcttaaggcaaatactggtcggtcagcagtaatagatgttggcactgtggagttaataaagaaaggggatatcaaggtctttggtacaatatcttgcatcaagggaaatgttgttgaatttgatgatgggaaagaaagttactttgatgctattgtttttgctacaggctacacaagtactgcaaacaactggcttaagaatggtgaggatatgatgaacaaagaaggcatgcccaagaaggacttcccaaatcactggaaaggttcaaatggactctactgtgttgggtttgcgaggagaggattatctggtattgctcatgacgccaaaaacgttgctaatgactggatggaagaagacttgacaccaaagcccgccgttggcagttctaagaacagtcagccctcacttatcacatccatggatggtgaacgatgctttgccttagtggcagagcaaactgtttccattggaggcaattga</cdnaseq>|
AA = <aaseq>MEKALVLIVGAGPSGLATAACLGQLSIPYVIIEREDCTASLWRK HTYDRLKLHLAKEFCEMPHMPYPEDTPTYIPKIQFLRYMDDYVEHFNICPKFNSSVES CLYDDVQKYWVVTTHDQVNGMVSKYAARFLVVASGENSAGNIPSIPGLEDFSGHVIHS SSFRSADSYAAQRVLVVGCGNSGMEIAYDLSSHGANTSIVIRSPLHVMTKELIHMGMK LASWSLPVKFVDFILVVLAYLWFGNLSKYGIVRPNKGPLLLKANTGRSAVIDVGTVEL IKKGDIKVFGTISCIKGNVVEFDDGKESYFDAIVFATGYTSTANNWLKNGEDMMNKEG MPKKDFPNHWKGSNGLYCVGFARRGLSGIAHDAKNVANDWMEEDLTPKPAVGSSKNSQ PSLITSMDGERCFALVAEQTVSIGGN</aaseq>|
DNA = <dnaseqindica>8513..8650#1491..1637#1069..1191#742..978#1..612#atggagaaggctctagttctgattgttggagcaggcccatctggtcttgcaacagcggcgtgccttggccagctctctataccctatgttattatcgagcgtgaggattgtaccgcgtcgctgtggcgtaagcacacatatgatcgcctcaagcttcacctcgcaaaggagttctgcgagatgccccacatgccatatccggaagacacaccaacctacatccccaagattcaatttttgaggtatatggatgattatgtggagcatttcaacatttgtcccaaatttaactcatctgttgagtcatgtttgtatgatgatgtgcaaaaatattgggttgtcacaacacatgaccaagtgaatggcatggtgtccaagtatgcagctaggttcttagttgtagcaagtggtgaaaatagtgcagggaacattcctagcatccctggactagaggatttctctggccatgtcatacactcatcaagctttaggtcagcagattcctatgctgcgcaaagggtgcttgttgttggatgtggaaactcaggcatggagattgcctatgatctttcaagccatggagcaaatacctccatagtcatccgcagccctgtaagtaaataatacatttgcataaaagtttcctttctttattttgcctccctgttttccaggtcaagttattgtaagtatcctattaaatcattttcttacaccacaaattcttgaattattctaaagctgcatgtgatgacaaaggagctgattcacatggggatgaaacttgcaagctggagcctcccagtgaaatttgttgattttattcttgtggtccttgcatatctctggtttggcaatctctcaaagtatggcatcgtgaggcccaataaggggccattgcttcttaaggcaaatactggtcggtcagcagtaatagatgttggcactgtggagttaataaagaaaggggatatcaaggtaacaaaattcttgacacaacatagaaaagtctatataaattctatttggtacttgacaggttctataaggaatttatgtggcatgtaggtctttggtacaatatcttgcatcaagggaaatgttgttgaatttgatgatgggaaagaaagttactttgatgctattgtttttgctacaggctacacaagtactgcaaacaactggcttaaggtatgtaatatgttgctcatatggtaaatagtaaatacaaaaattattgcaattagatttagttgttattttctctctcctgcatacctcaagtggggcatcaaattaagagtgcctacaatactattagacagatttagaaagaaaagcatttgcgtgattgactcaacaaaattagtgataaaacctgttataaaattatagttataattagaagacaatggtgctatcttcatatttaactttctttctttctgtggaaatatttgttaataaaataattatgattaaaaacgcagaatggtgaggatatgatgaacaaagaaggcatgcccaagaaggacttcccaaatcactggaaaggttcaaatggactctactgtgttgggtttgcgaggagaggattatctggtattgctcatgacgccaaaaacgttgctaatgacgtcaaggccttcctagatttgatggcaccattctaattattatttgcactggaatgatgcctatcaaatagtattgtggtaccttctacaatagtggtgtgtacttaatgttacattatgtttgaagcaagctaaatgttatgcaatctccagagttgtttaaataattatattttcttaatcgacatgttggatgagcttttatttaaagctctttgctggaatacatgaatatgtggtttattctttataatgtgtgaagccctttgtttggatccatgaaaaataaaatcagaaactcatgtcaaacctcccctcacacctgtattacctatcctactctaaatcataccccctcctccccccatcggcaccatagttgttcagccagtgacgccctgcccttttgaaagaagttgagcatcactgccggttcatggctgtttaggcgggaaaattcactccaacgccatgaactggtaaagattggcaatccctgttggctcacaccgaaccgatagagatcagtgggcaaggattgccctttctgtaccagtaacacctttatccactccagttatgaaaaaaaaatgctctttagagttgtttgttccttatttcaactcaacacaacaatatggagttctaaaaaataacacaacattatggagaatactaaataatagtagagatcatagattacttagaagttagaacatacctaaaagggagattttttttttaaaaaaagaattcattttcaatcctatgccttattttcactgacatttggaaagggagtagtatgtctatttggggaagaggttcttttttttttttggggggcggggggggggggggggagggggaagggagggctgctttgattaattcctcccattgtattacatttcacttggatggatcatacctcctattcttctagataccacgaacaaatgtcattgaattatgagatagactaaaatctgcgggtgtcaaagatttcagaaaacttgagtctttgatgtaaatgttggtggaaattgcacccttttatatcaaatgcccattgggtttgtgcagctagcctaaaaaagattattccccattttggaaggttatgtgttacaaatcagacatctccgcaatattttcagaaaactaaagtgggtactaatactatatttttgcatgatcctggcctaaggtggtattacaatctgttgagatatgcaatggtcaaagagtggtactgtacattctattagagagctagattttattttgaactctagatgtgggtaactgatgatactggattgacgaaagccgaaagcccatctttatgagttttttaaggacttggtaggatcaatatataaacttttaataggagaaaatgtacatttactataaccaatttttacatgacgcctcgacaggaggcacccctcccctaggcatgcctctaaagttagccctgttagtgttctatcccttttgagcagaccattcttgagtaggttacaaatgtcacactgtttgatactccatttgtcctaaaatataaggtggccatatatagttttagcaaaaccaacatatgtaggttttgacaaataacaaactaacaatatactgtatatgtttaatgccatccatttcatatcctaagattcatatttgaaagcactcttgtatgatgtaattttctagttcttgataatatattattgaagaaactaattgttaaaatataatattgtagacagtgtaagaaaactaatatgctttatagtttgagactgataggactattataaaaaggtcctcctattacacttgatgagaatgatggtaaaattgcatgatatataatttgtgaaagctggcacatattcaatgaaatcgatatattctttggatgatcacgggtgtttctgggccaaatttctactttatttgtaatcccgagagcttcatctacaagtaaaaaaaaaatccaggtttttagttgataatgtagtctatcttaataaaggaagagatgcaaaggaggaaatttcctgcaaacaccggaatgtcttttcttccctgactgaaaatacagttttgttctttacatgtaaattagctagcaaagttatttaggggcattacaggtttagatatgctttggcaccaaaaccaggtacagaacaaatgttcgctggatgcactgcatcgatcttgatgtactccctatgttcaattatataagacatttttggtttgttctaatcaaacttgaatatacatattttcagtttgaccaaacaatgtagtagtgtttccaacacaaaacaaatatactacagaaatatatttaatggtggatttaatgaaaccaataaagagtatgcgataatagtaagtccatctaatgcataaaatcctggtgcattactatcctaggttaatatggcaccacctatactaagatcttcttctttctcttcatgtttgattgtacatcttgatatttccttccatgtgcatgcatggtttcaacaaaaaaaaaggtaacactgacttgttccaataatacacattgtagttatctatagcccttttttacatacatattacccccattatgtttgcagttgttacccccctttatctgattagagcataaaggacctgtggaatttttactcttataacccgactttttttatctattccataacctacaaatgggtttcctattttgatttgtaaaaataacaaagagaaagcctttgctctgatggtcacaatccttcgatttattccttttgtttgtaataatgttagtaaagtaatccatcagttgatttataaccccctctcgccaatgaaactgattcacttttatgaagtgaggagaaagaagggatcaattaatcgaggatccaatattttagtccctcgaggaagtctcctaaatcattggtagtattcatttgactctactagttacaactaataagacatatttataagaaagaaaatgcagcaggaggattactgatttcaattagttactatgtgcaaaattcagtgctcgcgtgccggggggggggggggaggggcggcaacggagttattgttcctacatacaataaatagtgatgacatgctctagtgaagttatttcagactggatccacatgggtgtctttcttttgtttctttggaaaggcttgttgtagctatctcatcttgataggtcgaaagattcgttcgttagtaacgacttgcttaaacaaagtt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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001072867.2 RefSeq:Os12g0189500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 12]]
[[Category:Chromosome 12]]

Os06g0660200

2014-06-03T14:02:48Z

Hylwayne:

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsPIN2was proposed as an auxin efflux transporter and a member of the PIN family (Wanget al., 2009)<ref name="pmid:19825657" />. The cDNA
sequence of OsPIN2(AK101191) comprises 2276 nucleotides and alignment between the cDNA, and the genomic sequence
revealed that the gene has seven exons and six introns (Figure S1A). The ORF encodes a protein of 629 amino acid
residues and shows 56.3% sequence identity with Arabidopsis AtPIN2 .Hydropathy and transmembrane (TM) motif
analyses of the amino acid sequence revealed that the OsPIN2 protein contains three characteristic regions, including a
hydrophobic region with five TM domains, a predominantly hydrophilic core, followed by another hydrophobic region with
four TM segments .

'''The gain of function ofOsPIN2in rice plants'''

It was previously reported that knockdown of OsPIN1bexpression very significantly increased the number of tillers and tiller
angle, while over-expression did not alter the shoot architecture
of rice (Xuet al., 2005)<ref name="pmid:16085936" />. In the rice genome, OsLazy1 has been
identified as a novel grass-specific protein playing a negative
role in polar auxin transport; its mutation resulted in an increase
in the rice tiller angle (Abe et al., 1996<ref name="pmid:11539861" />; Li et al., 2007<ref name="pmid:17468779" />; Yoshihara and Iino, 2007<ref name="pmid:17412736" /> ). It is interesting to speculate whether
enhancingOsLazy1expression could compact rice architecture
further. In addition, OsTAC1was defined as a tiller angle
control gene in rice, its expression level positively correlated
with tiller angle and numbers (Yuet al., 2007))<ref name="pmid:17908158" />.
Transgenic plants over-expressingOsPIN2showed larger tiller
angles, lower plant height, more tillers than WT (Figure 2c–e),
which was similar to theOsPIN1bknockdown mutants (Xu
et al., 2005)<ref name="pmid:16085936" />. The change of phenotype caused by alteration of
OsPIN2andOsPIN1bexpression supported the common observation that rice plant height was negatively correlated with tiller
number (Wang and Li, 2005). Therefore, auxin might play a key
role in the crosstalk between the plant height and branching,
an aspect of canopy architecture which is poorly understood.

===Expression===
'''Generation of transgenic rice showingOsPIN2 over-expression and phenotypes of transformants'''
Ubiquitin promoter (pUbi) has been long reported as a useful
strong promoter in a variety of applications in gene transfer
studies and drives the gene expression most actively in rapidly
dividing cells (Cornejoet al., 1993)<ref name="pmid:8219091" /> . To investigate the function
ofOsPIN2in rice, we made transgenic rice plants over-expressing the full length of cDNA driven by pUbi. Southern blotting
analysis revealed that they came from two transgenic lines (O1
and O2, respectively) distinct from each other and both had
one copy of the transgene (Figure 1a). Real-time RT-PCR
analyses showed that transcriptional expression ofOsPIN2was
moderate in the root–shoot junctions and roots but very faint in
the leaves of WT (the untransformed plants as control)
(Figure 1b). In both the transgenic lines, OsPIN2had the most
abundant transcripts in the root–shoot junction, and similar
levels in the leaves and roots (Figure 1b). The increases in
OsPIN2transcriptional expression driven by pUbi in these tissues
reached by 3–15 times.

Over-expression ofOsPIN2significantly increased tiller angle
and numbers and decreased height of the rice in comparison
with WT (Figure 1i). At 80 days after germination,
O1 and O2 plants had a wider tiller angle (the angle between
the outermost tillers in the left and right side) (44° and 48°,
respectively) than the WT (22°). In addition, the two
transgenic lines had many more tillers per plant (21 and 23,
respectively) than the WT (17 on the average). In
contrast, the whole plant height was very significantly
decreased byOsPIN2over-expression. The transgenic
plant phenotypes were inheritable from T0 (Figure 2f–h) to T2
generations.
At the ripening stage, theOsPIN2-over-expressing
plants showed shorter panicle length, less number of grains per
panicle and lower grain weight per panicle in comparison with
the control. Even though the seed setting rate was
similar between O1, O2 and WT, O1 plants had a 35% increase
in the effective tiller number, leading to a 16% grain yield
increase per plant relative to control plants. O2 plants
showed the same tendency but not significant increase in panicle number and grain yield. OsPIN2over-expression
also decreased the grain length and breadth by 4%–7% and
12%–16%, respectively. The changes in the grain size
lead to 10%–12.5% decreases of 1000-grain weight compared
to WT. Moreover, over-expression of the OsPIN2significantly
decreased the number of adventitious roots and the total root
length by 22%–28%.

There was no putative gene in the insertion site for both O1 and O2. This direct genetic evidence further supported the OsPIN2 contribution to the altered
phenotype of the transgenic rice in Figure 1

[[File:Phenotypes and molecular analyses ofOsPIN2over-expression transgenic plants (O1 and O2) and untranformed wild-type plant (WT).jpg]]

'''Auxin transport altered byOsPIN2over-expression
in rice'''
To assess whether the over-expression ofOsPIN2affected the
free auxin levels, the concentration of endogenous free IAA in
various organs of the transgenic and WT plants was quantified
by high-performance liquid chromatography (HPLC). In the first
and second leaves from the top, O1 and O2 transgenic plants
contained free IAA concentrations that were 11%–35% lower
than that in the control plants (Figure 3a). In the sheath of the
first leaf, the transgenic plants and WT had nearly the same
concentration of free IAA, while in the sheath of the second
leaf, O1 and O2 plants had 38%–53% lower free IAA concentration than that in WT (Figure 3b). In contrast, in the root–
shoot junction (shoot base), the two transgenic lines contained
65%–128% more free IAA than that in WT (Figure 3c).
To detect the effect ofOsPIN2over-expression on auxin distribution, we separated individual roots into three segments:
0–4 cm including the root apex and elongation zones; 4–8 cm,
the lateral root area; and 8–12 cm which was adjacent to
the root–shoot junction. Both transgenic and WT roots showed
the same patterns of free IAA concentration with an abrupt
decrease from the tip to the root base (Figure 3d).

===Evolution===
'''Structure and evolution of the PIN super family'''

Genes homologous to the Arabidopsis PIN sequences are present in genomes throughout the plant kingdom. These include representatives in non-vascular plants such as Physcomitrella; in vascular plants, PIN sequences appear to be ubiquitous. Both before and since the divergence of monocot and dicot plants there have been significant changes in the number and the structure of PINs. However, in dicot plants, the phylogenetic structure of the family has been broadly conserved ( Figure 2a). Both Medicago and potato contain at least five PIN sequences (these data were collected from EST sequencing projects and, accordingly, have the propensity to exclude sequences from mRNA expressed either at a low level or exclusively in certain tissue types). Each of these genes shows similarity to one of the eight PINs of Arabidopsis. Both Medicago and potato contain two genes homologous to AtPIN1 and two genes homologous to the AtPIN3, AtPIN4, AtPIN7 group. Medicago has one gene homologous to AtPIN2. Potato has one gene homologous to AtPIN6. There is additional evidence for such a broadly conserved phylogenetic structure in the PIN genes of soybean (Glycine max). Here, there exist single homologous genes to AtPIN1, AtPIN2, AtPIN5, AtPIN8, and two genes homologous to the AtPIN3, AtPIN4, AtPIN7 cluster of Arabidopsis.

[[File:Neighbour-joining phylogenetic trees showing the predicted relationship between Arabidopsis AtPINs and (a) dicot (Medicago, soybean and potato) and (b) monocot (rice and wheat) PINs.jpg]]

To confirm that the changed phenotype of O1 and O2 was
caused by over-expression of OsPIN2in the transgenic plants,
we detected the location of one copy T-DNA insertion in these
two lines. The T-DNA containing ubi-promoter was inserted in
the chromosome 3 and 10 in the genome of O1 and O2,
respectively (Figure 2).

[[File:Schematic map of integrated T-DNA insertion site in rice genome.jpg]]

==Labs working on this gene==
1.State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing,
China

2.Institut für Biologie II, Universität Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany.

==References==
<references>
<ref name="pmid:19825657"> Wang, J.R., Hu, H., Wang, G.H., Li, J., Chen, J.Y. and Wu, P. (2009)
Expression of PIN Genes in Rice (Oryza sativaL.): tissue specificity and
regulation by hormones. Mol. Plant, 2, 823–831. </ref>
<ref name="pmid:16085936"> Xu, M., Zhu, L., Shou, H.X. and Wu, P. (2005) A PIN1 family gene, OsPIN1,
involved in auxin-dependent adventitious root emergence and tillering in
rice. Plant Cell Physiol.46, 1674–1681. </ref>
<ref name="pmid:17908158"> Yu, B., Lin, Z., Li, H., Li, X., Li, J., Wang, Y., Zhang, X., Zhu, Z., Zhai, W.,
Wang, X., Xie, D. and Sun, C. (2007) TAC1, a major quantitative trait
locus controlling tiller angle in rice. Plant J.52, 891–898.</ref>
<ref name="pmid:11539861"> Abe, K., Takahashi, H. and Suge, H. (1996) Lazy gene (la) responsible for
both an agravitropism of seedlings and lazy habit of tiller growth in rice
(Oryza sativaL).J. Plant. Res.109, 381–386. </ref>
<ref name="pmid:17468779"> Li, P., Wang, Y., Qian, Q., Fu, Z., Wang, M., Zeng, D., Li, B., Wang, X. and
Li, J. (2007) LAZY1 controls rice shoot gravitropism through regulating
polar auxin transport.Cell Res.17, 402–410.</ref>
<ref name="pmid:17412736"> Yoshihara, T. and Iino, M. (2007) Identification of the gravitropism-related
rice gene LAZY1 and elucidation of LAZY1-dependent and -independent
gravity signaling pathways.Plant Cell Physiol.48, 678–688 </ref>
<ref name="pmid:8219091"> Cornejo, M.J., Luth, D., Blankenship, K.M., Anderson, O.D. and Blechl, A.E.
(1993) Activity of a maize ubiquitin promoter in transgenic rice. Plant Mol.
Biol.23, 567–581.</ref>

</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0660200|
Description = Similar to Auxin efflux carrier protein|
Version = NM_001064803.1 GI:115469337 GeneID:4341736|
Length = 3804 bp|
Definition = Oryza sativa Japonica Group Os06g0660200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:28077811..28081614|
CDS = 28077886..28078901,28078996..28079180,28079269..28079572,28080611..28080696,28080796..28080953 ,28081075..28081151,28081241..28081307|
GCID = <gbrowseImage1>
name=NC_008399:28077811..28081614
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:28077811..28081614
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgatcaccggacgcgacatctacgacgtgctggcggcgatcgtgccgctgtacgtggcgatgttcctggcgtacgggtcggtgcggtggtgggggatattcacgccggaccagtgctccggcatcaaccgcttcgtcgccgtcttcgccgtgccgctcctctccttccacttcatctccaccaacgacccctactccatgaactaccgcttcctcgccgccgactcgctccagaagctcgtcatcctcgccgcgctcgccgtctggcacaacctgctctcccgctaccgccgcaatggcggcgccgccgcgtcgctcgactggaccatcaccctcttctcgctgtccacgctgcccaacacgctggtcatgggcatcccgctgctccgcgccatgtacggcgacttctccggctcgctcatggtgcagatcgtcgtgctccagagcgtcatctggtacaccctcatgctcttcctcttcgagtaccgcggcgccaaggcgctcatctccgagcagttcccgccggacgtcggcgccagcatcgcctcgttccgcgtcgactccgacgtcgtctcgctcaacgggagggaggcgctgcaggcggacgccgaggtggggcgcgacggccgcgtccacgtcgtcatccgccgctccgcctcggcctccaccacgggcggcggcggcggcgcggcgcgctccggcgtgtcccgggcgtacggcgcgtccaacgccatgacgccgcgcgcctccaacctcaccggcgtggagatctactcgctgcagacgtcgcgcgagcccacgccgcgggcgtccagcttcaaccaggccgacttctacgccatgttctccggcagcaagatggccagccagatggctagccccatggcgcagcacggcggcgccggcggccgcgcccagggcctcgacgagcaggtcaccaacaagttcgcctccggcaaggccgccgacccgccgtcgtatcccgccccgaaccccggcatgatgccggcgccaaggaagaaggagctcgggggctcaaactccaactccaacaaggagctacacatgttcgtgtggagctctagcgcgtcgccggtgtcggaggccaacctccgcaacgccgtcaaccacgccgcctccaccgacttcgcctccgcgccgccgccggcagccgttcccgtcggcggcgccactcccaaaggggtgagtggcagtgtcacgccggcggcgaagaacggcggcggcgagttggagatcgaggacgggctgaagagcccggcggcggggctggcggcgaagttcccggtgtcggggtcgccgtacgtggcgccgaggaagaagggcggcggcgccgacgtgcccgggctggcggaggcggcgcacccgatgccgccgacgagcgtgatgacgcggctcatcctcatcatggtgtggcgcaagctcatcagaaaccccaacacctactccagcctcatcggcctcgtctggtccctcgtctccttcaggtggaatatccaaatgccttcaataataaagggctcaatatcaatattgtcagatgcagggctaggaatggctatgttcagcttaggcttgttcatggctctgcaaccaaagatcatttcttgtggcaagaccgttgcgacatttgcaatggcagtgaggttcttgactggtccagctgttattgcagctacttccattgccattgggctcaggggagtactcttgcatgttgccattgttcaggcagcacttccacaaggcattgtcccgtttgtgtttgccaaggagtacaattgccatcctcaaatacttagcacagcggttatttttgggatgctcatcgcgcttccgatcacgatactctactatgtgcttcttgggatatag</cdnaseq>|
AA = <aaseq>MITGRDIYDVLAAIVPLYVAMFLAYGSVRWWGIFTPDQCSGINR FVAVFAVPLLSFHFISTNDPYSMNYRFLAADSLQKLVILAALAVWHNLLSRYRRNGGA AASLDWTITLFSLSTLPNTLVMGIPLLRAMYGDFSGSLMVQIVVLQSVIWYTLMLFLF EYRGAKALISEQFPPDVGASIASFRVDSDVVSLNGREALQADAEVGRDGRVHVVIRRS ASASTTGGGGGAARSGVSRAYGASNAMTPRASNLTGVEIYSLQTSREPTPRASSFNQA DFYAMFSGSKMASQMASPMAQHGGAGGRAQGLDEQVTNKFASGKAADPPSYPAPNPGM MPAPRKKELGGSNSNSNKELHMFVWSSSASPVSEANLRNAVNHAASTDFASAPPPAAV PVGGATPKGVSGSVTPAAKNGGGELEIEDGLKSPAAGLAAKFPVSGSPYVAPRKKGGG ADVPGLAEAAHPMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLVWSLVSFRWNIQMP SIIKGSISILSDAGLGMAMFSLGLFMALQPKIISCGKTVATFAMAVRFLTGPAVIAAT SIAIGLRGVLLHVAIVQAALPQGIVPFVFAKEYNCHPQILSTAVIFGMLIALPITILY YVLLGI</aaseq>|
DNA = <dnaseqindica>76..1091#1186..1370#1459..1762#2801..2886#2986..3143#3265..3341#3431..3497#aattagctcgttctcttgtgtcaagaaaaaaaaagaaagaaaaagctcgccgccgccgccaccgtcgccggcgcgatgatcaccggacgcgacatctacgacgtgctggcggcgatcgtgccgctgtacgtggcgatgttcctggcgtacgggtcggtgcggtggtgggggatattcacgccggaccagtgctccggcatcaaccgcttcgtcgccgtcttcgccgtgccgctcctctccttccacttcatctccaccaacgacccctactccatgaactaccgcttcctcgccgccgactcgctccagaagctcgtcatcctcgccgcgctcgccgtctggcacaacctgctctcccgctaccgccgcaatggcggcgccgccgcgtcgctcgactggaccatcaccctcttctcgctgtccacgctgcccaacacgctggtcatgggcatcccgctgctccgcgccatgtacggcgacttctccggctcgctcatggtgcagatcgtcgtgctccagagcgtcatctggtacaccctcatgctcttcctcttcgagtaccgcggcgccaaggcgctcatctccgagcagttcccgccggacgtcggcgccagcatcgcctcgttccgcgtcgactccgacgtcgtctcgctcaacgggagggaggcgctgcaggcggacgccgaggtggggcgcgacggccgcgtccacgtcgtcatccgccgctccgcctcggcctccaccacgggcggcggcggcggcgcggcgcgctccggcgtgtcccgggcgtacggcgcgtccaacgccatgacgccgcgcgcctccaacctcaccggcgtggagatctactcgctgcagacgtcgcgcgagcccacgccgcgggcgtccagcttcaaccaggccgacttctacgccatgttctccggcagcaagatggccagccagatggctagccccatggcgcagcacggcggcgccggcggccgcgcccagggcctcgacgagcaggtcaccaacaagttcgcctccggcaaggccgccgacccgccgtcgtatcccgccccgaaccccggcatgatgccggcgccaaggtaaaatgaaactgattattaacacctcaaaatttctgttcatcgatcgtgttttgatcgagttggatttttgattttgtcgccattgctacaggaagaaggagctcgggggctcaaactccaactccaacaaggagctacacatgttcgtgtggagctctagcgcgtcgccggtgtcggaggccaacctccgcaacgccgtcaaccacgccgcctccaccgacttcgcctccgcgccgccgccggcagccgttcccgtcggcggcgccactcccaaaggtactctctcatccatccattgacgcacacgacgacgatcacaccacatgtgttcaaggcttcgtctaatggtgcgtgcatgccacaggggtgagtggcagtgtcacgccggcggcgaagaacggcggcggcgagttggagatcgaggacgggctgaagagcccggcggcggggctggcggcgaagttcccggtgtcggggtcgccgtacgtggcgccgaggaagaagggcggcggcgccgacgtgcccgggctggcggaggcggcgcacccgatgccgccgacgagcgtgatgacgcggctcatcctcatcatggtgtggcgcaagctcatcagaaaccccaacacctactccagcctcatcggcctcgtctggtccctcgtctccttcaggtattggattctcaccgatgttttcggtactcaagctgacgttctcgcttccacagttccacttcatcgacgcccgctttcccggtcgcttccctctaagatggaacactcttctgccaatacattttgacatcccacagctatgacagatcgcttatcccggttcatcttcagggcaagggcgctttttttcaagttataccgaattatcaaaacagattatttactctccatttcaaaaaagtattttataaaaaaaaatattcttctactacattacttagattacttgttttaagctagttgttaagtttttttttaaattgtttaatttatttgtataatgaaatcgataatctgcatagtaatctaaaccaaataagtaatatacacttgagttcacgatctgatcaatttatgttgttcgaagctacaaaaaggatatatttttgctatgctattcgttgctagattgcttgaaattttaatctaggaagaggcaagatcgatgagaacttacttaacttcacaactggattagtgttttctgaacgcatgtgagagcacatgataattacgttggtctcggatctggtccagcacatgcaaagaacatacacacgtatgcagatgcaggtgcaggtgcaggtcttatgtgagccctggagtttagcagcagagaccaaatctaccgaatcagctgcgattgccgtgtttgtccccatccaagacagacacggcgaattcggctcaactagctagatgatccatcatgtgtacaagaagaagagcaaaaaatgtacaatgatatggatgcatcattgattcattgtactactatcttaccattagatggcacatctagagggagggttgcaagaacagggcatttttagtggggaggagcacatgcctgcaagcatctaaaattgcatacatgcatgatacatctagcattgattctcttgtcatacaatttttttttacattgtgaaaatatatgcttaattgcttattgactttgtgaatttggatcaggtggaatatccaaatgccttcaataataaagggctcaatatcaatattgtcagatgcagggctaggaatggctatgttcagcttaggtacagagttgacctttttgatacatatctatagccataactagtgagttttttctttgctttgaccaagaaattctgacaatagtatatgtaacccaggcttgttcatggctctgcaaccaaagatcatttcttgtggcaagaccgttgcgacatttgcaatggcagtgaggttcttgactggtccagctgttattgcagctacttccattgccattgggctcaggggagtactcttgcatgttgccattgttcaggtaagcagtagattctttatttcttgcaatcatcttaaaagaaaaaaaatattaaattttgtaattatggtgaattggaatgtattgtggtgatcttatagattaattgattgtcaaacaggcagcacttccacaaggcattgtcccgtttgtgtttgccaaggagtacaattgccatcctcaaatacttagcacagcgtaagaaatgcatgcttaaactttctatttgttcgtccatacggttgctgaagtatatagcctaaaatatataaatgtgaaattttcagggttatttttgggatgctcatcgcgcttccgatcacgatactctactatgtgcttcttgggatatagtgttcttgaagaaggcaaaaaagaaagagtagggaaaaaaataggattctaggtttctagaggaaaatgcaaaagaaatatgatatgggctttcttgaagacctgaagaactaccagagctgaagaatagggaaatgagatcaagtaggatcctagctagagagaaatgcaaaggaaagacaccccttgattacaattttttaattttttctgcaactgttttggcatcaaagtaaaggttagggccttgagtatgaagagttcagccgttaatttgacaagttgggttggcggtactaaagattcc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064803.1 RefSeq:Os06g0660200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os02g0743400

2014-06-03T13:51:23Z

Hylwayne: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==
===Function===

OsPIN1 was expressed in the vascular tissues and root primordial in a manner similar to AtPIN1. Adventitious root emergence and development were significantly inhibited in the OsPIN1 RNA interference (RNAi) transgenic plants, which was similar to the phenotype of NPA (N-1-naphthylphalamic acid, an auxintransport inhibitor)-treated wild-type plants. α-naphthylacetic acid (α-NAA) treatment was able to rescue the mutated phenotypes occurring in the RNAi plants. Overexpression or suppression of the OsPIN1 expression through a transgenic approach resulted in changes of tiller numbers and shoot/root ratio. Taken together, these data suggest that OsPIN1 plays an important role in auxindependent adventitious root emergence and tillering<ref name="pmid:16085936" /><ref name="pmid:14651850" /><ref name="pmid:15635403" />.

Five adventitious roots were formed at the coleoptile node of 7-day-old seedlings in both the RNAi transgenic and the wild-type plants. In the second week, numbers of adventitious roots developed from the first node of the wildtype plants, while no new adventitious roots were initiated until the third week in the RNAi transgenic plants. Moreover, the number of adventitious roots in the RNAi transgenic plants was significantly less than in the wild-type plants.

Results showed that adventitious root primordia were initiated in 7-day-old seedlings of both wild-type and OsPIN1 RNAi transgenic plants, indicating that the formation of adventitious root primordia at the first node was not affected by the underexpression of OsPIN1. However, in the 14-day-old seedlings, new adventitious roots had developed in the wildtype plants, while they were not visible in the OsPIN1 RNAi transgenic plants. The adventitious root primordial in these RNAi plants stayed at the same size as those in the 7-day-old seedlings, indicating that the emergence of adventitious root primordia was arrested in these RNAi seedlings. Data showed that the development of adventitious roots in RNAi seedlings ceased at the sixth stage.

[[File:Adventitious roots in OsPIN1 transgenic plants.jpg]]

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

[[File: Gene structure and characterization of OsPIN1(1).jpg]]
[[File: Gene structure and characterization of OsPIN1(2).jpg]]

You can also add sub-section(s) at will.

==Labs working on this gene==

State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Kaixuan Road, Hangzhou, Zhejiang
310029, PR China

==References==

<references>
<ref name="pmid:16085936"> Xu M, Zhu L, Shou H, Wu P. A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice. Plant Cell Physiol. 2005 Oct;46(10):1674-81. </ref>
<ref name="pmid:14651850"> Benková E, Michniewicz M, Sauer M, Teichmann T, Seifertová D, Jürgens G, Friml J. Local, efflux-dependent auxin gradients as a common module for plant organ formation. Cell. 2003 Nov 26;115(5):591-602. </ref>
<ref name="pmid:15635403"> Blilou I1, Xu J, Wildwater M, Willemsen V, Paponov I, Friml J, Heidstra R, Aida M, Palme K, Scheres B.The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots. Nature. 2005 Jan 6;433(7021):39-44. </ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0743400|
Description = Auxin transport protein REH1|
Version = NM_001054630.1 GI:115448630 GeneID:4330700|
Length = 3075 bp|
Definition = Oryza sativa Japonica Group Os02g0743400, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:32045781..32048855|
CDS = 32046245..32046311,32046396..32046472,32046764..32046921,32047015..32047100,32047215..32047488 ,32047585..32048710|
GCID = <gbrowseImage1>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaatggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagctgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaa</cdnaseq>|
AA = <aaseq>MITAADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINR FVALFAVPLLSFHFISTNNPYTMNLRFIAADTLQKLMVLAMLTAWSHLSRRGSLEWTI TLFSLSTLPNTLVMGIPLLKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARML ITEQFPDTAANIASIVVDPDVVSLDGRRDAIETETEVKEDGRIHVTVRRSNASRSDIY SRRSMGFSSTTPRPSNLTNAEIYSLQSSRNPTPRGSSFNHTDFYSMVGRSSNFGAADA FGVRTGATPRPSNYEDDASKPKYPLPASNAAPMAGHYPAPNPAVSSAPKGAKKAATNG QAKGEDLHMFVWSSSASPVSDVFGGGAPDYNDAAAVKSPRKMDGAKDREDYVERDDFS FGNRGVMDRDAEAGDEKAAAAAGADPSKAMAAPTAMPPTSVMTRLILIMVWRKLIRNP NTYSSLIGLIWSLVCFRWNFEMPAIVLKSISILSDAGLGMAMFSLGLFMALQPHIIAC GNKVATYAMAVRFLAGPAVMAAASFAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSV HPSILSTAVIFGMLIALPITLVYYILLGL</aaseq>|
DNA = <dnaseqindica>2545..2611#2384..2460#1935..2092#1756..1841#1368..1641#146..1271#gcgcacacacccaatcaaatgctcctcctccgccgcctcctctcgctgagctgagctgagctgtgaaatagtgccaccgagtgagcgctgagctgagctcgaagcaagagaggaagaggaggaggagggaagagggggggcgaagatgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaagtagcaatcttttatcttcaccgtgtactatttgcctgatttggggagcatttcttgggctaatttgtactgactcgtatatttcttttacgtcagtggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtgcgtacaccccgggcttctcgcaaacccaaacccatctgctcgtacgtgctgtggtggtgcggtgtggtggtctggttctgacaaggcgttcgtctcgttttggcgttgcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtcggtattctcttgtttccatgctaacctcacctcacccccggcagtgtcacgagctgttcccctgagtttgcgatctcgccatgcatgcaggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggtaaaccggaacggaaaagaacagccatacgcacacactgtcacacgcacagtcacacaccgaaaataaaggtctccgtgtgggtgggcatgcaacctaccgcgcgcagccagccagcgtcgttcttttctcccagcccctcgtgctcgttcgcgtctgctttctctcctgccttttgctctccacggccgcggcactttctacgtttggccagcacgaaagccttttgctttggctaacctttttctctctgttgttcttttgtttggtccaaaccgaacgaacgaacaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagcgtaagtactagattctctctcagtttgcactctgtcctgaatcgacgggccactgacattttttgcctttgctcctgctcgcagtgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaatcgagttgcatgcatgtaaattcctgctcctgacaaccagccatgttaagaagaggggagaagaagacagagctggtacactgtttgcaaagtcaggactctttgatttttcttttcttttctgtatttcttgaagtagaatttgggaggagggggattggaagggagtcaaacgagtcaaggggaggacaggatggtagctagcttagctaggacaatggtgagtcacaaaagagcaccaaaagcaagtacaagtacaaagcttggggggacacaggatccagttcaggtcacagaaacggttcggttttgggaggggattgtgggagttttggttggctgcgctgcgctgacccttgtaaaacggacgccgattctgacaagagatcgaccttgttttgttcgtgtcgtggtattgctgactactactttggaatgattaatctcctactactaattactcc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054630.1 RefSeq:Os02g0743400]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0743400

2014-06-03T13:50:32Z

Hylwayne: /* Evolution */

Please input one-sentence summary here.

==Annotated Information==
===Function===

OsPIN1 was expressed in the vascular tissues and root primordial in a manner similar to AtPIN1. Adventitious root emergence and development were significantly inhibited in the OsPIN1 RNA interference (RNAi) transgenic plants, which was similar to the phenotype of NPA (N-1-naphthylphalamic acid, an auxintransport inhibitor)-treated wild-type plants. α-naphthylacetic acid (α-NAA) treatment was able to rescue the mutated phenotypes occurring in the RNAi plants. Overexpression or suppression of the OsPIN1 expression through a transgenic approach resulted in changes of tiller numbers and shoot/root ratio. Taken together, these data suggest that OsPIN1 plays an important role in auxindependent adventitious root emergence and tillering<ref name="pmid:16085936" /><ref name="pmid:14651850" /><ref name="pmid:15635403" />.

Five adventitious roots were formed at the coleoptile node of 7-day-old seedlings in both the RNAi transgenic and the wild-type plants. In the second week, numbers of adventitious roots developed from the first node of the wildtype plants, while no new adventitious roots were initiated until the third week in the RNAi transgenic plants. Moreover, the number of adventitious roots in the RNAi transgenic plants was significantly less than in the wild-type plants.

Results showed that adventitious root primordia were initiated in 7-day-old seedlings of both wild-type and OsPIN1 RNAi transgenic plants, indicating that the formation of adventitious root primordia at the first node was not affected by the underexpression of OsPIN1. However, in the 14-day-old seedlings, new adventitious roots had developed in the wildtype plants, while they were not visible in the OsPIN1 RNAi transgenic plants. The adventitious root primordial in these RNAi plants stayed at the same size as those in the 7-day-old seedlings, indicating that the emergence of adventitious root primordia was arrested in these RNAi seedlings. Data showed that the development of adventitious roots in RNAi seedlings ceased at the sixth stage.

[[File:Adventitious roots in OsPIN1 transgenic plants.jpg]]

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

[[File: Gene structure and characterization of OsPIN1(1).jpg]]
[[File: Gene structure and characterization of OsPIN1(2).jpg]]

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==

<references>
<ref name="pmid:16085936"> Xu M, Zhu L, Shou H, Wu P. A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice. Plant Cell Physiol. 2005 Oct;46(10):1674-81. </ref>
<ref name="pmid:14651850"> Benková E, Michniewicz M, Sauer M, Teichmann T, Seifertová D, Jürgens G, Friml J. Local, efflux-dependent auxin gradients as a common module for plant organ formation. Cell. 2003 Nov 26;115(5):591-602. </ref>
<ref name="pmid:15635403"> Blilou I1, Xu J, Wildwater M, Willemsen V, Paponov I, Friml J, Heidstra R, Aida M, Palme K, Scheres B.The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots. Nature. 2005 Jan 6;433(7021):39-44. </ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0743400|
Description = Auxin transport protein REH1|
Version = NM_001054630.1 GI:115448630 GeneID:4330700|
Length = 3075 bp|
Definition = Oryza sativa Japonica Group Os02g0743400, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:32045781..32048855|
CDS = 32046245..32046311,32046396..32046472,32046764..32046921,32047015..32047100,32047215..32047488 ,32047585..32048710|
GCID = <gbrowseImage1>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaatggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagctgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaa</cdnaseq>|
AA = <aaseq>MITAADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINR FVALFAVPLLSFHFISTNNPYTMNLRFIAADTLQKLMVLAMLTAWSHLSRRGSLEWTI TLFSLSTLPNTLVMGIPLLKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARML ITEQFPDTAANIASIVVDPDVVSLDGRRDAIETETEVKEDGRIHVTVRRSNASRSDIY SRRSMGFSSTTPRPSNLTNAEIYSLQSSRNPTPRGSSFNHTDFYSMVGRSSNFGAADA FGVRTGATPRPSNYEDDASKPKYPLPASNAAPMAGHYPAPNPAVSSAPKGAKKAATNG QAKGEDLHMFVWSSSASPVSDVFGGGAPDYNDAAAVKSPRKMDGAKDREDYVERDDFS FGNRGVMDRDAEAGDEKAAAAAGADPSKAMAAPTAMPPTSVMTRLILIMVWRKLIRNP NTYSSLIGLIWSLVCFRWNFEMPAIVLKSISILSDAGLGMAMFSLGLFMALQPHIIAC GNKVATYAMAVRFLAGPAVMAAASFAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSV HPSILSTAVIFGMLIALPITLVYYILLGL</aaseq>|
DNA = <dnaseqindica>2545..2611#2384..2460#1935..2092#1756..1841#1368..1641#146..1271#gcgcacacacccaatcaaatgctcctcctccgccgcctcctctcgctgagctgagctgagctgtgaaatagtgccaccgagtgagcgctgagctgagctcgaagcaagagaggaagaggaggaggagggaagagggggggcgaagatgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaagtagcaatcttttatcttcaccgtgtactatttgcctgatttggggagcatttcttgggctaatttgtactgactcgtatatttcttttacgtcagtggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtgcgtacaccccgggcttctcgcaaacccaaacccatctgctcgtacgtgctgtggtggtgcggtgtggtggtctggttctgacaaggcgttcgtctcgttttggcgttgcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtcggtattctcttgtttccatgctaacctcacctcacccccggcagtgtcacgagctgttcccctgagtttgcgatctcgccatgcatgcaggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggtaaaccggaacggaaaagaacagccatacgcacacactgtcacacgcacagtcacacaccgaaaataaaggtctccgtgtgggtgggcatgcaacctaccgcgcgcagccagccagcgtcgttcttttctcccagcccctcgtgctcgttcgcgtctgctttctctcctgccttttgctctccacggccgcggcactttctacgtttggccagcacgaaagccttttgctttggctaacctttttctctctgttgttcttttgtttggtccaaaccgaacgaacgaacaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagcgtaagtactagattctctctcagtttgcactctgtcctgaatcgacgggccactgacattttttgcctttgctcctgctcgcagtgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaatcgagttgcatgcatgtaaattcctgctcctgacaaccagccatgttaagaagaggggagaagaagacagagctggtacactgtttgcaaagtcaggactctttgatttttcttttcttttctgtatttcttgaagtagaatttgggaggagggggattggaagggagtcaaacgagtcaaggggaggacaggatggtagctagcttagctaggacaatggtgagtcacaaaagagcaccaaaagcaagtacaagtacaaagcttggggggacacaggatccagttcaggtcacagaaacggttcggttttgggaggggattgtgggagttttggttggctgcgctgcgctgacccttgtaaaacggacgccgattctgacaagagatcgaccttgttttgttcgtgtcgtggtattgctgactactactttggaatgattaatctcctactactaattactcc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054630.1 RefSeq:Os02g0743400]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0743400

2014-06-03T12:50:14Z

Hylwayne: /* Function */

Please input one-sentence summary here.

==Annotated Information==
===Function===

OsPIN1 was expressed in the vascular tissues and root primordial in a manner similar to AtPIN1. Adventitious root emergence and development were significantly inhibited in the OsPIN1 RNA interference (RNAi) transgenic plants, which was similar to the phenotype of NPA (N-1-naphthylphalamic acid, an auxintransport inhibitor)-treated wild-type plants. α-naphthylacetic acid (α-NAA) treatment was able to rescue the mutated phenotypes occurring in the RNAi plants. Overexpression or suppression of the OsPIN1 expression through a transgenic approach resulted in changes of tiller numbers and shoot/root ratio. Taken together, these data suggest that OsPIN1 plays an important role in auxindependent adventitious root emergence and tillering<ref name="pmid:16085936" /><ref name="pmid:14651850" /><ref name="pmid:15635403" />.

Five adventitious roots were formed at the coleoptile node of 7-day-old seedlings in both the RNAi transgenic and the wild-type plants. In the second week, numbers of adventitious roots developed from the first node of the wildtype plants, while no new adventitious roots were initiated until the third week in the RNAi transgenic plants. Moreover, the number of adventitious roots in the RNAi transgenic plants was significantly less than in the wild-type plants.

Results showed that adventitious root primordia were initiated in 7-day-old seedlings of both wild-type and OsPIN1 RNAi transgenic plants, indicating that the formation of adventitious root primordia at the first node was not affected by the underexpression of OsPIN1. However, in the 14-day-old seedlings, new adventitious roots had developed in the wildtype plants, while they were not visible in the OsPIN1 RNAi transgenic plants. The adventitious root primordial in these RNAi plants stayed at the same size as those in the 7-day-old seedlings, indicating that the emergence of adventitious root primordia was arrested in these RNAi seedlings. Data showed that the development of adventitious roots in RNAi seedlings ceased at the sixth stage.

[[File:Adventitious roots in OsPIN1 transgenic plants.jpg]]

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==

<references>
<ref name="pmid:16085936"> Xu M, Zhu L, Shou H, Wu P. A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice. Plant Cell Physiol. 2005 Oct;46(10):1674-81. </ref>
<ref name="pmid:14651850"> Benková E, Michniewicz M, Sauer M, Teichmann T, Seifertová D, Jürgens G, Friml J. Local, efflux-dependent auxin gradients as a common module for plant organ formation. Cell. 2003 Nov 26;115(5):591-602. </ref>
<ref name="pmid:15635403"> Blilou I1, Xu J, Wildwater M, Willemsen V, Paponov I, Friml J, Heidstra R, Aida M, Palme K, Scheres B.The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots. Nature. 2005 Jan 6;433(7021):39-44. </ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0743400|
Description = Auxin transport protein REH1|
Version = NM_001054630.1 GI:115448630 GeneID:4330700|
Length = 3075 bp|
Definition = Oryza sativa Japonica Group Os02g0743400, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:32045781..32048855|
CDS = 32046245..32046311,32046396..32046472,32046764..32046921,32047015..32047100,32047215..32047488 ,32047585..32048710|
GCID = <gbrowseImage1>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaatggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagctgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaa</cdnaseq>|
AA = <aaseq>MITAADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINR FVALFAVPLLSFHFISTNNPYTMNLRFIAADTLQKLMVLAMLTAWSHLSRRGSLEWTI TLFSLSTLPNTLVMGIPLLKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARML ITEQFPDTAANIASIVVDPDVVSLDGRRDAIETETEVKEDGRIHVTVRRSNASRSDIY SRRSMGFSSTTPRPSNLTNAEIYSLQSSRNPTPRGSSFNHTDFYSMVGRSSNFGAADA FGVRTGATPRPSNYEDDASKPKYPLPASNAAPMAGHYPAPNPAVSSAPKGAKKAATNG QAKGEDLHMFVWSSSASPVSDVFGGGAPDYNDAAAVKSPRKMDGAKDREDYVERDDFS FGNRGVMDRDAEAGDEKAAAAAGADPSKAMAAPTAMPPTSVMTRLILIMVWRKLIRNP NTYSSLIGLIWSLVCFRWNFEMPAIVLKSISILSDAGLGMAMFSLGLFMALQPHIIAC GNKVATYAMAVRFLAGPAVMAAASFAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSV HPSILSTAVIFGMLIALPITLVYYILLGL</aaseq>|
DNA = <dnaseqindica>2545..2611#2384..2460#1935..2092#1756..1841#1368..1641#146..1271#gcgcacacacccaatcaaatgctcctcctccgccgcctcctctcgctgagctgagctgagctgtgaaatagtgccaccgagtgagcgctgagctgagctcgaagcaagagaggaagaggaggaggagggaagagggggggcgaagatgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaagtagcaatcttttatcttcaccgtgtactatttgcctgatttggggagcatttcttgggctaatttgtactgactcgtatatttcttttacgtcagtggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtgcgtacaccccgggcttctcgcaaacccaaacccatctgctcgtacgtgctgtggtggtgcggtgtggtggtctggttctgacaaggcgttcgtctcgttttggcgttgcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtcggtattctcttgtttccatgctaacctcacctcacccccggcagtgtcacgagctgttcccctgagtttgcgatctcgccatgcatgcaggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggtaaaccggaacggaaaagaacagccatacgcacacactgtcacacgcacagtcacacaccgaaaataaaggtctccgtgtgggtgggcatgcaacctaccgcgcgcagccagccagcgtcgttcttttctcccagcccctcgtgctcgttcgcgtctgctttctctcctgccttttgctctccacggccgcggcactttctacgtttggccagcacgaaagccttttgctttggctaacctttttctctctgttgttcttttgtttggtccaaaccgaacgaacgaacaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagcgtaagtactagattctctctcagtttgcactctgtcctgaatcgacgggccactgacattttttgcctttgctcctgctcgcagtgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaatcgagttgcatgcatgtaaattcctgctcctgacaaccagccatgttaagaagaggggagaagaagacagagctggtacactgtttgcaaagtcaggactctttgatttttcttttcttttctgtatttcttgaagtagaatttgggaggagggggattggaagggagtcaaacgagtcaaggggaggacaggatggtagctagcttagctaggacaatggtgagtcacaaaagagcaccaaaagcaagtacaagtacaaagcttggggggacacaggatccagttcaggtcacagaaacggttcggttttgggaggggattgtgggagttttggttggctgcgctgcgctgacccttgtaaaacggacgccgattctgacaagagatcgaccttgttttgttcgtgtcgtggtattgctgactactactttggaatgattaatctcctactactaattactcc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054630.1 RefSeq:Os02g0743400]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0743400

2014-06-03T12:47:10Z

Hylwayne: /* Function */

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsPIN1 was expressed in the vascular tissues and root primordial in a manner similar to AtPIN1. Adventitious root emergence and development were significantly inhibited in the OsPIN1 RNA interference (RNAi) transgenic plants, which was similar to the phenotype of NPA (N-1-naphthylphalamic acid, an auxintransport inhibitor)-treated wild-type plants. α-naphthylacetic
acid (α-NAA) treatment was able to rescue the mutated phenotypes occurring in the RNAi plants. Overexpression or suppression of the OsPIN1 expression through a transgenic approach resulted in changes of tiller numbers and shoot/root ratio. Taken together, these data suggest that OsPIN1 plays an important role in auxindependent adventitious root emergence and tillering<ref name="pmid:16085936" /><ref name="pmid:14651850" /><ref name="pmid:15635403" />.
Five adventitious roots were formed at the coleoptile node of 7-day-old seedlings in both the RNAi transgenic and the wild-type plants. In the second week, numbers of adventitious roots developed from the first node of the wildtype plants, while no new adventitious roots were initiated until the third week in the RNAi transgenic plants. Moreover, the number of adventitious roots in the RNAi transgenic plants was significantly less than in the wild-type plants.
Results showed that adventitious root primordia were initiated in 7-day-old seedlings of both wild-type and OsPIN1 RNAi transgenic plants, indicating that the formation of adventitious root primordia at the first node was not affected by the underexpression of OsPIN1. However, in the 14-day-old seedlings, new adventitious roots had developed in the wildtype
plants, while they were not visible in the OsPIN1 RNAi transgenic plants. The adventitious root primordial in these RNAi plants stayed at the same size as those in the 7-day-old seedlings, indicating that the emergence of adventitious root primordia was arrested in these RNAi seedlings. Data showed that the development of adventitious roots in RNAi seedlings ceased at the sixth stage.

[[File:Adventitious roots in OsPIN1 transgenic plants.jpg]]

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==

<references>
<ref name="pmid:16085936"> Xu M, Zhu L, Shou H, Wu P. A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice. Plant Cell Physiol. 2005 Oct;46(10):1674-81. </ref>
<ref name="pmid:14651850"> Benková E, Michniewicz M, Sauer M, Teichmann T, Seifertová D, Jürgens G, Friml J. Local, efflux-dependent auxin gradients as a common module for plant organ formation. Cell. 2003 Nov 26;115(5):591-602. </ref>
<ref name="pmid:15635403"> Blilou I1, Xu J, Wildwater M, Willemsen V, Paponov I, Friml J, Heidstra R, Aida M, Palme K, Scheres B.The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots. Nature. 2005 Jan 6;433(7021):39-44. </ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0743400|
Description = Auxin transport protein REH1|
Version = NM_001054630.1 GI:115448630 GeneID:4330700|
Length = 3075 bp|
Definition = Oryza sativa Japonica Group Os02g0743400, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:32045781..32048855|
CDS = 32046245..32046311,32046396..32046472,32046764..32046921,32047015..32047100,32047215..32047488 ,32047585..32048710|
GCID = <gbrowseImage1>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaatggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagctgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaa</cdnaseq>|
AA = <aaseq>MITAADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINR FVALFAVPLLSFHFISTNNPYTMNLRFIAADTLQKLMVLAMLTAWSHLSRRGSLEWTI TLFSLSTLPNTLVMGIPLLKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARML ITEQFPDTAANIASIVVDPDVVSLDGRRDAIETETEVKEDGRIHVTVRRSNASRSDIY SRRSMGFSSTTPRPSNLTNAEIYSLQSSRNPTPRGSSFNHTDFYSMVGRSSNFGAADA FGVRTGATPRPSNYEDDASKPKYPLPASNAAPMAGHYPAPNPAVSSAPKGAKKAATNG QAKGEDLHMFVWSSSASPVSDVFGGGAPDYNDAAAVKSPRKMDGAKDREDYVERDDFS FGNRGVMDRDAEAGDEKAAAAAGADPSKAMAAPTAMPPTSVMTRLILIMVWRKLIRNP NTYSSLIGLIWSLVCFRWNFEMPAIVLKSISILSDAGLGMAMFSLGLFMALQPHIIAC GNKVATYAMAVRFLAGPAVMAAASFAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSV HPSILSTAVIFGMLIALPITLVYYILLGL</aaseq>|
DNA = <dnaseqindica>2545..2611#2384..2460#1935..2092#1756..1841#1368..1641#146..1271#gcgcacacacccaatcaaatgctcctcctccgccgcctcctctcgctgagctgagctgagctgtgaaatagtgccaccgagtgagcgctgagctgagctcgaagcaagagaggaagaggaggaggagggaagagggggggcgaagatgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaagtagcaatcttttatcttcaccgtgtactatttgcctgatttggggagcatttcttgggctaatttgtactgactcgtatatttcttttacgtcagtggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtgcgtacaccccgggcttctcgcaaacccaaacccatctgctcgtacgtgctgtggtggtgcggtgtggtggtctggttctgacaaggcgttcgtctcgttttggcgttgcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtcggtattctcttgtttccatgctaacctcacctcacccccggcagtgtcacgagctgttcccctgagtttgcgatctcgccatgcatgcaggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggtaaaccggaacggaaaagaacagccatacgcacacactgtcacacgcacagtcacacaccgaaaataaaggtctccgtgtgggtgggcatgcaacctaccgcgcgcagccagccagcgtcgttcttttctcccagcccctcgtgctcgttcgcgtctgctttctctcctgccttttgctctccacggccgcggcactttctacgtttggccagcacgaaagccttttgctttggctaacctttttctctctgttgttcttttgtttggtccaaaccgaacgaacgaacaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagcgtaagtactagattctctctcagtttgcactctgtcctgaatcgacgggccactgacattttttgcctttgctcctgctcgcagtgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaatcgagttgcatgcatgtaaattcctgctcctgacaaccagccatgttaagaagaggggagaagaagacagagctggtacactgtttgcaaagtcaggactctttgatttttcttttcttttctgtatttcttgaagtagaatttgggaggagggggattggaagggagtcaaacgagtcaaggggaggacaggatggtagctagcttagctaggacaatggtgagtcacaaaagagcaccaaaagcaagtacaagtacaaagcttggggggacacaggatccagttcaggtcacagaaacggttcggttttgggaggggattgtgggagttttggttggctgcgctgcgctgacccttgtaaaacggacgccgattctgacaagagatcgaccttgttttgttcgtgtcgtggtattgctgactactactttggaatgattaatctcctactactaattactcc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054630.1 RefSeq:Os02g0743400]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0743400

2014-06-03T12:41:27Z

Hylwayne:

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsPIN1 was expressed in the vascular tissues and root primordial in a manner similar to AtPIN1. Adventitious root emergence and development were significantly inhibited in the OsPIN1 RNA interference (RNAi) transgenic plants, which was similar to the phenotype of NPA (N-1-naphthylphalamic acid, an auxintransport inhibitor)-treated wild-type plants. α-naphthylacetic
acid (α-NAA) treatment was able to rescue the mutated phenotypes occurring in the RNAi plants. Overexpression or suppression of the OsPIN1 expression through a transgenic approach resulted in changes of tiller numbers and shoot/root ratio. Taken together, these data suggest that OsPIN1 plays an important role in auxindependent adventitious root emergence and tillering<ref name="pmid:16085936" /><ref name="pmid:14651850" /><ref name="pmid:15635403" />.
[[File:Adventitious roots in OsPIN1 transgenic plants.jpg]]

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==

<references>
<ref name="pmid:16085936"> Xu M, Zhu L, Shou H, Wu P. A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice. Plant Cell Physiol. 2005 Oct;46(10):1674-81. </ref>
<ref name="pmid:14651850"> Benková E, Michniewicz M, Sauer M, Teichmann T, Seifertová D, Jürgens G, Friml J. Local, efflux-dependent auxin gradients as a common module for plant organ formation. Cell. 2003 Nov 26;115(5):591-602. </ref>
<ref name="pmid:15635403"> Blilou I1, Xu J, Wildwater M, Willemsen V, Paponov I, Friml J, Heidstra R, Aida M, Palme K, Scheres B.The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots. Nature. 2005 Jan 6;433(7021):39-44. </ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0743400|
Description = Auxin transport protein REH1|
Version = NM_001054630.1 GI:115448630 GeneID:4330700|
Length = 3075 bp|
Definition = Oryza sativa Japonica Group Os02g0743400, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:32045781..32048855|
CDS = 32046245..32046311,32046396..32046472,32046764..32046921,32047015..32047100,32047215..32047488 ,32047585..32048710|
GCID = <gbrowseImage1>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaatggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagctgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaa</cdnaseq>|
AA = <aaseq>MITAADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINR FVALFAVPLLSFHFISTNNPYTMNLRFIAADTLQKLMVLAMLTAWSHLSRRGSLEWTI TLFSLSTLPNTLVMGIPLLKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARML ITEQFPDTAANIASIVVDPDVVSLDGRRDAIETETEVKEDGRIHVTVRRSNASRSDIY SRRSMGFSSTTPRPSNLTNAEIYSLQSSRNPTPRGSSFNHTDFYSMVGRSSNFGAADA FGVRTGATPRPSNYEDDASKPKYPLPASNAAPMAGHYPAPNPAVSSAPKGAKKAATNG QAKGEDLHMFVWSSSASPVSDVFGGGAPDYNDAAAVKSPRKMDGAKDREDYVERDDFS FGNRGVMDRDAEAGDEKAAAAAGADPSKAMAAPTAMPPTSVMTRLILIMVWRKLIRNP NTYSSLIGLIWSLVCFRWNFEMPAIVLKSISILSDAGLGMAMFSLGLFMALQPHIIAC GNKVATYAMAVRFLAGPAVMAAASFAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSV HPSILSTAVIFGMLIALPITLVYYILLGL</aaseq>|
DNA = <dnaseqindica>2545..2611#2384..2460#1935..2092#1756..1841#1368..1641#146..1271#gcgcacacacccaatcaaatgctcctcctccgccgcctcctctcgctgagctgagctgagctgtgaaatagtgccaccgagtgagcgctgagctgagctcgaagcaagagaggaagaggaggaggagggaagagggggggcgaagatgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaagtagcaatcttttatcttcaccgtgtactatttgcctgatttggggagcatttcttgggctaatttgtactgactcgtatatttcttttacgtcagtggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtgcgtacaccccgggcttctcgcaaacccaaacccatctgctcgtacgtgctgtggtggtgcggtgtggtggtctggttctgacaaggcgttcgtctcgttttggcgttgcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtcggtattctcttgtttccatgctaacctcacctcacccccggcagtgtcacgagctgttcccctgagtttgcgatctcgccatgcatgcaggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggtaaaccggaacggaaaagaacagccatacgcacacactgtcacacgcacagtcacacaccgaaaataaaggtctccgtgtgggtgggcatgcaacctaccgcgcgcagccagccagcgtcgttcttttctcccagcccctcgtgctcgttcgcgtctgctttctctcctgccttttgctctccacggccgcggcactttctacgtttggccagcacgaaagccttttgctttggctaacctttttctctctgttgttcttttgtttggtccaaaccgaacgaacgaacaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagcgtaagtactagattctctctcagtttgcactctgtcctgaatcgacgggccactgacattttttgcctttgctcctgctcgcagtgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaatcgagttgcatgcatgtaaattcctgctcctgacaaccagccatgttaagaagaggggagaagaagacagagctggtacactgtttgcaaagtcaggactctttgatttttcttttcttttctgtatttcttgaagtagaatttgggaggagggggattggaagggagtcaaacgagtcaaggggaggacaggatggtagctagcttagctaggacaatggtgagtcacaaaagagcaccaaaagcaagtacaagtacaaagcttggggggacacaggatccagttcaggtcacagaaacggttcggttttgggaggggattgtgggagttttggttggctgcgctgcgctgacccttgtaaaacggacgccgattctgacaagagatcgaccttgttttgttcgtgtcgtggtattgctgactactactttggaatgattaatctcctactactaattactcc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054630.1 RefSeq:Os02g0743400]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0743400

2014-06-03T12:30:53Z

Hylwayne:

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsPIN1 was expressed in the vascular tissues and root primordial in a manner similar to AtPIN1. Adventitious root emergence and development were significantly inhibited in the OsPIN1 RNA interference (RNAi) transgenic plants, which was similar to the phenotype of NPA (N-1-naphthylphalamic acid, an auxintransport inhibitor)-treated wild-type plants. α-naphthylacetic
acid (α-NAA) treatment was able to rescue the mutated phenotypes occurring in the RNAi plants. Overexpression or suppression of the OsPIN1 expression through a transgenic approach resulted in changes of tiller numbers and shoot/root ratio. Taken together, these data suggest that OsPIN1 plays an important role in auxindependent adventitious root emergence and tillering<ref name="pmid:16085936" />.
[[File:Adventitious roots in OsPIN1 transgenic plants.jpg]]

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==

<references>
<ref name="pmid:16085936"> Xu M, Zhu L, Shou H, Wu P. A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice. Plant Cell Physiol. 2005 Oct;46(10):1674-81. </ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0743400|
Description = Auxin transport protein REH1|
Version = NM_001054630.1 GI:115448630 GeneID:4330700|
Length = 3075 bp|
Definition = Oryza sativa Japonica Group Os02g0743400, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:32045781..32048855|
CDS = 32046245..32046311,32046396..32046472,32046764..32046921,32047015..32047100,32047215..32047488 ,32047585..32048710|
GCID = <gbrowseImage1>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaatggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagctgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaa</cdnaseq>|
AA = <aaseq>MITAADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINR FVALFAVPLLSFHFISTNNPYTMNLRFIAADTLQKLMVLAMLTAWSHLSRRGSLEWTI TLFSLSTLPNTLVMGIPLLKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARML ITEQFPDTAANIASIVVDPDVVSLDGRRDAIETETEVKEDGRIHVTVRRSNASRSDIY SRRSMGFSSTTPRPSNLTNAEIYSLQSSRNPTPRGSSFNHTDFYSMVGRSSNFGAADA FGVRTGATPRPSNYEDDASKPKYPLPASNAAPMAGHYPAPNPAVSSAPKGAKKAATNG QAKGEDLHMFVWSSSASPVSDVFGGGAPDYNDAAAVKSPRKMDGAKDREDYVERDDFS FGNRGVMDRDAEAGDEKAAAAAGADPSKAMAAPTAMPPTSVMTRLILIMVWRKLIRNP NTYSSLIGLIWSLVCFRWNFEMPAIVLKSISILSDAGLGMAMFSLGLFMALQPHIIAC GNKVATYAMAVRFLAGPAVMAAASFAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSV HPSILSTAVIFGMLIALPITLVYYILLGL</aaseq>|
DNA = <dnaseqindica>2545..2611#2384..2460#1935..2092#1756..1841#1368..1641#146..1271#gcgcacacacccaatcaaatgctcctcctccgccgcctcctctcgctgagctgagctgagctgtgaaatagtgccaccgagtgagcgctgagctgagctcgaagcaagagaggaagaggaggaggagggaagagggggggcgaagatgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaagtagcaatcttttatcttcaccgtgtactatttgcctgatttggggagcatttcttgggctaatttgtactgactcgtatatttcttttacgtcagtggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtgcgtacaccccgggcttctcgcaaacccaaacccatctgctcgtacgtgctgtggtggtgcggtgtggtggtctggttctgacaaggcgttcgtctcgttttggcgttgcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtcggtattctcttgtttccatgctaacctcacctcacccccggcagtgtcacgagctgttcccctgagtttgcgatctcgccatgcatgcaggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggtaaaccggaacggaaaagaacagccatacgcacacactgtcacacgcacagtcacacaccgaaaataaaggtctccgtgtgggtgggcatgcaacctaccgcgcgcagccagccagcgtcgttcttttctcccagcccctcgtgctcgttcgcgtctgctttctctcctgccttttgctctccacggccgcggcactttctacgtttggccagcacgaaagccttttgctttggctaacctttttctctctgttgttcttttgtttggtccaaaccgaacgaacgaacaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagcgtaagtactagattctctctcagtttgcactctgtcctgaatcgacgggccactgacattttttgcctttgctcctgctcgcagtgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaatcgagttgcatgcatgtaaattcctgctcctgacaaccagccatgttaagaagaggggagaagaagacagagctggtacactgtttgcaaagtcaggactctttgatttttcttttcttttctgtatttcttgaagtagaatttgggaggagggggattggaagggagtcaaacgagtcaaggggaggacaggatggtagctagcttagctaggacaatggtgagtcacaaaagagcaccaaaagcaagtacaagtacaaagcttggggggacacaggatccagttcaggtcacagaaacggttcggttttgggaggggattgtgggagttttggttggctgcgctgcgctgacccttgtaaaacggacgccgattctgacaagagatcgaccttgttttgttcgtgtcgtggtattgctgactactactttggaatgattaatctcctactactaattactcc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054630.1 RefSeq:Os02g0743400]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0743400

2014-06-03T12:26:43Z

Hylwayne:

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsPIN1 was expressed in the vascular tissues and root primordial in a manner similar to AtPIN1. Adventitious root emergence and development were significantly inhibited in the OsPIN1 RNA interference (RNAi) transgenic plants, which was similar to the phenotype of NPA (N-1-naphthylphalamic acid, an auxintransport inhibitor)-treated wild-type plants. α-naphthylacetic
acid (α-NAA) treatment was able to rescue the mutated phenotypes occurring in the RNAi plants. Overexpression or suppression of the OsPIN1 expression through a transgenic approach resulted in changes of tiller numbers and shoot/root ratio. Taken together, these data suggest that OsPIN1 plays an important role in auxindependent adventitious root emergence and tillering<ref name="pmid:16085936" />.
[[File:Adventitious roots in OsPIN1 transgenic plants.jpg]]

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
<ref name="pmid:16085936"> Xu M, Zhu L, Shou H, Wu P. A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice. Plant Cell Physiol. 2005 Oct;46(10):1674-81. </ref>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0743400|
Description = Auxin transport protein REH1|
Version = NM_001054630.1 GI:115448630 GeneID:4330700|
Length = 3075 bp|
Definition = Oryza sativa Japonica Group Os02g0743400, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:32045781..32048855|
CDS = 32046245..32046311,32046396..32046472,32046764..32046921,32047015..32047100,32047215..32047488 ,32047585..32048710|
GCID = <gbrowseImage1>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaatggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagctgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaa</cdnaseq>|
AA = <aaseq>MITAADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINR FVALFAVPLLSFHFISTNNPYTMNLRFIAADTLQKLMVLAMLTAWSHLSRRGSLEWTI TLFSLSTLPNTLVMGIPLLKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARML ITEQFPDTAANIASIVVDPDVVSLDGRRDAIETETEVKEDGRIHVTVRRSNASRSDIY SRRSMGFSSTTPRPSNLTNAEIYSLQSSRNPTPRGSSFNHTDFYSMVGRSSNFGAADA FGVRTGATPRPSNYEDDASKPKYPLPASNAAPMAGHYPAPNPAVSSAPKGAKKAATNG QAKGEDLHMFVWSSSASPVSDVFGGGAPDYNDAAAVKSPRKMDGAKDREDYVERDDFS FGNRGVMDRDAEAGDEKAAAAAGADPSKAMAAPTAMPPTSVMTRLILIMVWRKLIRNP NTYSSLIGLIWSLVCFRWNFEMPAIVLKSISILSDAGLGMAMFSLGLFMALQPHIIAC GNKVATYAMAVRFLAGPAVMAAASFAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSV HPSILSTAVIFGMLIALPITLVYYILLGL</aaseq>|
DNA = <dnaseqindica>2545..2611#2384..2460#1935..2092#1756..1841#1368..1641#146..1271#gcgcacacacccaatcaaatgctcctcctccgccgcctcctctcgctgagctgagctgagctgtgaaatagtgccaccgagtgagcgctgagctgagctcgaagcaagagaggaagaggaggaggagggaagagggggggcgaagatgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaagtagcaatcttttatcttcaccgtgtactatttgcctgatttggggagcatttcttgggctaatttgtactgactcgtatatttcttttacgtcagtggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtgcgtacaccccgggcttctcgcaaacccaaacccatctgctcgtacgtgctgtggtggtgcggtgtggtggtctggttctgacaaggcgttcgtctcgttttggcgttgcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtcggtattctcttgtttccatgctaacctcacctcacccccggcagtgtcacgagctgttcccctgagtttgcgatctcgccatgcatgcaggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggtaaaccggaacggaaaagaacagccatacgcacacactgtcacacgcacagtcacacaccgaaaataaaggtctccgtgtgggtgggcatgcaacctaccgcgcgcagccagccagcgtcgttcttttctcccagcccctcgtgctcgttcgcgtctgctttctctcctgccttttgctctccacggccgcggcactttctacgtttggccagcacgaaagccttttgctttggctaacctttttctctctgttgttcttttgtttggtccaaaccgaacgaacgaacaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagcgtaagtactagattctctctcagtttgcactctgtcctgaatcgacgggccactgacattttttgcctttgctcctgctcgcagtgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaatcgagttgcatgcatgtaaattcctgctcctgacaaccagccatgttaagaagaggggagaagaagacagagctggtacactgtttgcaaagtcaggactctttgatttttcttttcttttctgtatttcttgaagtagaatttgggaggagggggattggaagggagtcaaacgagtcaaggggaggacaggatggtagctagcttagctaggacaatggtgagtcacaaaagagcaccaaaagcaagtacaagtacaaagcttggggggacacaggatccagttcaggtcacagaaacggttcggttttgggaggggattgtgggagttttggttggctgcgctgcgctgacccttgtaaaacggacgccgattctgacaagagatcgaccttgttttgttcgtgtcgtggtattgctgactactactttggaatgattaatctcctactactaattactcc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054630.1 RefSeq:Os02g0743400]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0743400

2014-06-03T12:21:49Z

Hylwayne:

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsPIN1 was expressed in the vascular tissues and root primordial in a manner similar to AtPIN1. Adventitious root emergence and development were significantly inhibited in the OsPIN1 RNA interference (RNAi) transgenic plants, which was similar to the phenotype of NPA (N-1-naphthylphalamic acid, an auxintransport inhibitor)-treated wild-type plants. α-naphthylacetic
acid (α-NAA) treatment was able to rescue the mutated phenotypes occurring in the RNAi plants. Overexpression or suppression of the OsPIN1 expression through a transgenic approach resulted in changes of tiller numbers and shoot/root ratio. Taken together, these data suggest that OsPIN1 plays an important role in auxindependent adventitious root emergence and tillering<ref name="pmid:16085936" />.
[[File:Adventitious roots in OsPIN1 transgenic plants.jpg]]

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
<ref name="pmid:16085936"> Xu M, Zhu L, Shou H, Wu P. A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice. Plant Cell Physiol. 2005 Oct;46(10):1674-81.</ref>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0743400|
Description = Auxin transport protein REH1|
Version = NM_001054630.1 GI:115448630 GeneID:4330700|
Length = 3075 bp|
Definition = Oryza sativa Japonica Group Os02g0743400, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:32045781..32048855|
CDS = 32046245..32046311,32046396..32046472,32046764..32046921,32047015..32047100,32047215..32047488 ,32047585..32048710|
GCID = <gbrowseImage1>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaatggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagctgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaa</cdnaseq>|
AA = <aaseq>MITAADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINR FVALFAVPLLSFHFISTNNPYTMNLRFIAADTLQKLMVLAMLTAWSHLSRRGSLEWTI TLFSLSTLPNTLVMGIPLLKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARML ITEQFPDTAANIASIVVDPDVVSLDGRRDAIETETEVKEDGRIHVTVRRSNASRSDIY SRRSMGFSSTTPRPSNLTNAEIYSLQSSRNPTPRGSSFNHTDFYSMVGRSSNFGAADA FGVRTGATPRPSNYEDDASKPKYPLPASNAAPMAGHYPAPNPAVSSAPKGAKKAATNG QAKGEDLHMFVWSSSASPVSDVFGGGAPDYNDAAAVKSPRKMDGAKDREDYVERDDFS FGNRGVMDRDAEAGDEKAAAAAGADPSKAMAAPTAMPPTSVMTRLILIMVWRKLIRNP NTYSSLIGLIWSLVCFRWNFEMPAIVLKSISILSDAGLGMAMFSLGLFMALQPHIIAC GNKVATYAMAVRFLAGPAVMAAASFAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSV HPSILSTAVIFGMLIALPITLVYYILLGL</aaseq>|
DNA = <dnaseqindica>2545..2611#2384..2460#1935..2092#1756..1841#1368..1641#146..1271#gcgcacacacccaatcaaatgctcctcctccgccgcctcctctcgctgagctgagctgagctgtgaaatagtgccaccgagtgagcgctgagctgagctcgaagcaagagaggaagaggaggaggagggaagagggggggcgaagatgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaagtagcaatcttttatcttcaccgtgtactatttgcctgatttggggagcatttcttgggctaatttgtactgactcgtatatttcttttacgtcagtggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtgcgtacaccccgggcttctcgcaaacccaaacccatctgctcgtacgtgctgtggtggtgcggtgtggtggtctggttctgacaaggcgttcgtctcgttttggcgttgcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtcggtattctcttgtttccatgctaacctcacctcacccccggcagtgtcacgagctgttcccctgagtttgcgatctcgccatgcatgcaggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggtaaaccggaacggaaaagaacagccatacgcacacactgtcacacgcacagtcacacaccgaaaataaaggtctccgtgtgggtgggcatgcaacctaccgcgcgcagccagccagcgtcgttcttttctcccagcccctcgtgctcgttcgcgtctgctttctctcctgccttttgctctccacggccgcggcactttctacgtttggccagcacgaaagccttttgctttggctaacctttttctctctgttgttcttttgtttggtccaaaccgaacgaacgaacaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagcgtaagtactagattctctctcagtttgcactctgtcctgaatcgacgggccactgacattttttgcctttgctcctgctcgcagtgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaatcgagttgcatgcatgtaaattcctgctcctgacaaccagccatgttaagaagaggggagaagaagacagagctggtacactgtttgcaaagtcaggactctttgatttttcttttcttttctgtatttcttgaagtagaatttgggaggagggggattggaagggagtcaaacgagtcaaggggaggacaggatggtagctagcttagctaggacaatggtgagtcacaaaagagcaccaaaagcaagtacaagtacaaagcttggggggacacaggatccagttcaggtcacagaaacggttcggttttgggaggggattgtgggagttttggttggctgcgctgcgctgacccttgtaaaacggacgccgattctgacaagagatcgaccttgttttgttcgtgtcgtggtattgctgactactactttggaatgattaatctcctactactaattactcc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054630.1 RefSeq:Os02g0743400]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0743400

2014-06-03T12:19:03Z

Hylwayne: /* References */

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsPIN1 was expressed in the vascular tissues and root primordial in a manner similar to AtPIN1. Adventitious root emergence and development were significantly inhibited in the OsPIN1 RNA interference (RNAi) transgenic plants, which was similar to the phenotype of NPA (N-1-naphthylphalamic acid, an auxintransport inhibitor)-treated wild-type plants. α-naphthylacetic
acid (α-NAA) treatment was able to rescue the mutated phenotypes occurring in the RNAi plants. Overexpression or suppression of the OsPIN1 expression through a transgenic approach resulted in changes of tiller numbers and shoot/root ratio. Taken together, these data suggest that OsPIN1 plays an important role in auxindependent adventitious root emergence and tillering<ref name="pmid:16085936" />.
[[File:Adventitious roots in OsPIN1 transgenic plants.jpg]]

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
<ref name="pmid:16085936"> Xu M, Zhu L, Shou H, Wu P. A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice. Plant Cell Physiol. 2005 Oct;46(10):1674-81.</ref>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0743400|
Description = Auxin transport protein REH1|
Version = NM_001054630.1 GI:115448630 GeneID:4330700|
Length = 3075 bp|
Definition = Oryza sativa Japonica Group Os02g0743400, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:32045781..32048855|
CDS = 32046245..32046311,32046396..32046472,32046764..32046921,32047015..32047100,32047215..32047488 ,32047585..32048710|
GCID = <gbrowseImage1>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaatggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagctgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaa</cdnaseq>|
AA = <aaseq>MITAADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINR FVALFAVPLLSFHFISTNNPYTMNLRFIAADTLQKLMVLAMLTAWSHLSRRGSLEWTI TLFSLSTLPNTLVMGIPLLKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARML ITEQFPDTAANIASIVVDPDVVSLDGRRDAIETETEVKEDGRIHVTVRRSNASRSDIY SRRSMGFSSTTPRPSNLTNAEIYSLQSSRNPTPRGSSFNHTDFYSMVGRSSNFGAADA FGVRTGATPRPSNYEDDASKPKYPLPASNAAPMAGHYPAPNPAVSSAPKGAKKAATNG QAKGEDLHMFVWSSSASPVSDVFGGGAPDYNDAAAVKSPRKMDGAKDREDYVERDDFS FGNRGVMDRDAEAGDEKAAAAAGADPSKAMAAPTAMPPTSVMTRLILIMVWRKLIRNP NTYSSLIGLIWSLVCFRWNFEMPAIVLKSISILSDAGLGMAMFSLGLFMALQPHIIAC GNKVATYAMAVRFLAGPAVMAAASFAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSV HPSILSTAVIFGMLIALPITLVYYILLGL</aaseq>|
DNA = <dnaseqindica>2545..2611#2384..2460#1935..2092#1756..1841#1368..1641#146..1271#gcgcacacacccaatcaaatgctcctcctccgccgcctcctctcgctgagctgagctgagctgtgaaatagtgccaccgagtgagcgctgagctgagctcgaagcaagagaggaagaggaggaggagggaagagggggggcgaagatgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaagtagcaatcttttatcttcaccgtgtactatttgcctgatttggggagcatttcttgggctaatttgtactgactcgtatatttcttttacgtcagtggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtgcgtacaccccgggcttctcgcaaacccaaacccatctgctcgtacgtgctgtggtggtgcggtgtggtggtctggttctgacaaggcgttcgtctcgttttggcgttgcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtcggtattctcttgtttccatgctaacctcacctcacccccggcagtgtcacgagctgttcccctgagtttgcgatctcgccatgcatgcaggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggtaaaccggaacggaaaagaacagccatacgcacacactgtcacacgcacagtcacacaccgaaaataaaggtctccgtgtgggtgggcatgcaacctaccgcgcgcagccagccagcgtcgttcttttctcccagcccctcgtgctcgttcgcgtctgctttctctcctgccttttgctctccacggccgcggcactttctacgtttggccagcacgaaagccttttgctttggctaacctttttctctctgttgttcttttgtttggtccaaaccgaacgaacgaacaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagcgtaagtactagattctctctcagtttgcactctgtcctgaatcgacgggccactgacattttttgcctttgctcctgctcgcagtgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaatcgagttgcatgcatgtaaattcctgctcctgacaaccagccatgttaagaagaggggagaagaagacagagctggtacactgtttgcaaagtcaggactctttgatttttcttttcttttctgtatttcttgaagtagaatttgggaggagggggattggaagggagtcaaacgagtcaaggggaggacaggatggtagctagcttagctaggacaatggtgagtcacaaaagagcaccaaaagcaagtacaagtacaaagcttggggggacacaggatccagttcaggtcacagaaacggttcggttttgggaggggattgtgggagttttggttggctgcgctgcgctgacccttgtaaaacggacgccgattctgacaagagatcgaccttgttttgttcgtgtcgtggtattgctgactactactttggaatgattaatctcctactactaattactcc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054630.1 RefSeq:Os02g0743400]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0743400

2014-06-03T12:17:34Z

Hylwayne:

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsPIN1 was expressed in the vascular tissues and root primordial in a manner similar to AtPIN1. Adventitious root emergence and development were significantly inhibited in the OsPIN1 RNA interference (RNAi) transgenic plants, which was similar to the phenotype of NPA (N-1-naphthylphalamic acid, an auxintransport inhibitor)-treated wild-type plants. α-naphthylacetic
acid (α-NAA) treatment was able to rescue the mutated phenotypes occurring in the RNAi plants. Overexpression or suppression of the OsPIN1 expression through a transgenic approach resulted in changes of tiller numbers and shoot/root ratio. Taken together, these data suggest that OsPIN1 plays an important role in auxindependent adventitious root emergence and tillering<ref name="pmid:16085936" />.
[[File:Adventitious roots in OsPIN1 transgenic plants.jpg]]

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
<ref name="pmid:16085936"> Xu M1, Zhu L, Shou H, Wu P. A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice. Plant Cell Physiol. 2005 Oct;46(10):1674-81. Epub 2005 Aug 6.</ref>

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0743400|
Description = Auxin transport protein REH1|
Version = NM_001054630.1 GI:115448630 GeneID:4330700|
Length = 3075 bp|
Definition = Oryza sativa Japonica Group Os02g0743400, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:32045781..32048855|
CDS = 32046245..32046311,32046396..32046472,32046764..32046921,32047015..32047100,32047215..32047488 ,32047585..32048710|
GCID = <gbrowseImage1>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaatggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagctgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaa</cdnaseq>|
AA = <aaseq>MITAADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINR FVALFAVPLLSFHFISTNNPYTMNLRFIAADTLQKLMVLAMLTAWSHLSRRGSLEWTI TLFSLSTLPNTLVMGIPLLKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARML ITEQFPDTAANIASIVVDPDVVSLDGRRDAIETETEVKEDGRIHVTVRRSNASRSDIY SRRSMGFSSTTPRPSNLTNAEIYSLQSSRNPTPRGSSFNHTDFYSMVGRSSNFGAADA FGVRTGATPRPSNYEDDASKPKYPLPASNAAPMAGHYPAPNPAVSSAPKGAKKAATNG QAKGEDLHMFVWSSSASPVSDVFGGGAPDYNDAAAVKSPRKMDGAKDREDYVERDDFS FGNRGVMDRDAEAGDEKAAAAAGADPSKAMAAPTAMPPTSVMTRLILIMVWRKLIRNP NTYSSLIGLIWSLVCFRWNFEMPAIVLKSISILSDAGLGMAMFSLGLFMALQPHIIAC GNKVATYAMAVRFLAGPAVMAAASFAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSV HPSILSTAVIFGMLIALPITLVYYILLGL</aaseq>|
DNA = <dnaseqindica>2545..2611#2384..2460#1935..2092#1756..1841#1368..1641#146..1271#gcgcacacacccaatcaaatgctcctcctccgccgcctcctctcgctgagctgagctgagctgtgaaatagtgccaccgagtgagcgctgagctgagctcgaagcaagagaggaagaggaggaggagggaagagggggggcgaagatgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaagtagcaatcttttatcttcaccgtgtactatttgcctgatttggggagcatttcttgggctaatttgtactgactcgtatatttcttttacgtcagtggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtgcgtacaccccgggcttctcgcaaacccaaacccatctgctcgtacgtgctgtggtggtgcggtgtggtggtctggttctgacaaggcgttcgtctcgttttggcgttgcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtcggtattctcttgtttccatgctaacctcacctcacccccggcagtgtcacgagctgttcccctgagtttgcgatctcgccatgcatgcaggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggtaaaccggaacggaaaagaacagccatacgcacacactgtcacacgcacagtcacacaccgaaaataaaggtctccgtgtgggtgggcatgcaacctaccgcgcgcagccagccagcgtcgttcttttctcccagcccctcgtgctcgttcgcgtctgctttctctcctgccttttgctctccacggccgcggcactttctacgtttggccagcacgaaagccttttgctttggctaacctttttctctctgttgttcttttgtttggtccaaaccgaacgaacgaacaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagcgtaagtactagattctctctcagtttgcactctgtcctgaatcgacgggccactgacattttttgcctttgctcctgctcgcagtgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaatcgagttgcatgcatgtaaattcctgctcctgacaaccagccatgttaagaagaggggagaagaagacagagctggtacactgtttgcaaagtcaggactctttgatttttcttttcttttctgtatttcttgaagtagaatttgggaggagggggattggaagggagtcaaacgagtcaaggggaggacaggatggtagctagcttagctaggacaatggtgagtcacaaaagagcaccaaaagcaagtacaagtacaaagcttggggggacacaggatccagttcaggtcacagaaacggttcggttttgggaggggattgtgggagttttggttggctgcgctgcgctgacccttgtaaaacggacgccgattctgacaagagatcgaccttgttttgttcgtgtcgtggtattgctgactactactttggaatgattaatctcctactactaattactcc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054630.1 RefSeq:Os02g0743400]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0743400

2014-06-03T12:03:40Z

Hylwayne: /* Function */

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsPIN1 was expressed in the vascular tissues and root primordial in a manner similar to AtPIN1. Adventitious root emergence and development were significantly inhibited in the OsPIN1 RNA interference (RNAi) transgenic plants, which was similar to the phenotype of NPA (N-1-naphthylphalamic acid, an auxintransport inhibitor)-treated wild-type plants. α-naphthylacetic
acid (α-NAA) treatment was able to rescue the mutated phenotypes occurring in the RNAi plants. Overexpression or suppression of the OsPIN1 expression through a transgenic approach resulted in changes of tiller numbers and shoot/root ratio. Taken together, these data suggest that OsPIN1 plays an important role in auxindependent adventitious root emergence and tillering.
[[File:Adventitious roots in OsPIN1 transgenic plants.jpg]]

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0743400|
Description = Auxin transport protein REH1|
Version = NM_001054630.1 GI:115448630 GeneID:4330700|
Length = 3075 bp|
Definition = Oryza sativa Japonica Group Os02g0743400, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:32045781..32048855|
CDS = 32046245..32046311,32046396..32046472,32046764..32046921,32047015..32047100,32047215..32047488 ,32047585..32048710|
GCID = <gbrowseImage1>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaatggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagctgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaa</cdnaseq>|
AA = <aaseq>MITAADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINR FVALFAVPLLSFHFISTNNPYTMNLRFIAADTLQKLMVLAMLTAWSHLSRRGSLEWTI TLFSLSTLPNTLVMGIPLLKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARML ITEQFPDTAANIASIVVDPDVVSLDGRRDAIETETEVKEDGRIHVTVRRSNASRSDIY SRRSMGFSSTTPRPSNLTNAEIYSLQSSRNPTPRGSSFNHTDFYSMVGRSSNFGAADA FGVRTGATPRPSNYEDDASKPKYPLPASNAAPMAGHYPAPNPAVSSAPKGAKKAATNG QAKGEDLHMFVWSSSASPVSDVFGGGAPDYNDAAAVKSPRKMDGAKDREDYVERDDFS FGNRGVMDRDAEAGDEKAAAAAGADPSKAMAAPTAMPPTSVMTRLILIMVWRKLIRNP NTYSSLIGLIWSLVCFRWNFEMPAIVLKSISILSDAGLGMAMFSLGLFMALQPHIIAC GNKVATYAMAVRFLAGPAVMAAASFAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSV HPSILSTAVIFGMLIALPITLVYYILLGL</aaseq>|
DNA = <dnaseqindica>2545..2611#2384..2460#1935..2092#1756..1841#1368..1641#146..1271#gcgcacacacccaatcaaatgctcctcctccgccgcctcctctcgctgagctgagctgagctgtgaaatagtgccaccgagtgagcgctgagctgagctcgaagcaagagaggaagaggaggaggagggaagagggggggcgaagatgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaagtagcaatcttttatcttcaccgtgtactatttgcctgatttggggagcatttcttgggctaatttgtactgactcgtatatttcttttacgtcagtggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtgcgtacaccccgggcttctcgcaaacccaaacccatctgctcgtacgtgctgtggtggtgcggtgtggtggtctggttctgacaaggcgttcgtctcgttttggcgttgcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtcggtattctcttgtttccatgctaacctcacctcacccccggcagtgtcacgagctgttcccctgagtttgcgatctcgccatgcatgcaggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggtaaaccggaacggaaaagaacagccatacgcacacactgtcacacgcacagtcacacaccgaaaataaaggtctccgtgtgggtgggcatgcaacctaccgcgcgcagccagccagcgtcgttcttttctcccagcccctcgtgctcgttcgcgtctgctttctctcctgccttttgctctccacggccgcggcactttctacgtttggccagcacgaaagccttttgctttggctaacctttttctctctgttgttcttttgtttggtccaaaccgaacgaacgaacaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagcgtaagtactagattctctctcagtttgcactctgtcctgaatcgacgggccactgacattttttgcctttgctcctgctcgcagtgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaatcgagttgcatgcatgtaaattcctgctcctgacaaccagccatgttaagaagaggggagaagaagacagagctggtacactgtttgcaaagtcaggactctttgatttttcttttcttttctgtatttcttgaagtagaatttgggaggagggggattggaagggagtcaaacgagtcaaggggaggacaggatggtagctagcttagctaggacaatggtgagtcacaaaagagcaccaaaagcaagtacaagtacaaagcttggggggacacaggatccagttcaggtcacagaaacggttcggttttgggaggggattgtgggagttttggttggctgcgctgcgctgacccttgtaaaacggacgccgattctgacaagagatcgaccttgttttgttcgtgtcgtggtattgctgactactactttggaatgattaatctcctactactaattactcc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054630.1 RefSeq:Os02g0743400]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0743400

2014-06-03T11:57:54Z

Hylwayne: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsPIN1 was expressed in the vascular tissues and root primordial in a manner similar to AtPIN1. Adventitious root emergence and development were significantly inhibited in the OsPIN1 RNA interference (RNAi) transgenic plants, which was similar to the phenotype of NPA (N-1-naphthylphalamic acid, an auxintransport inhibitor)-treated wild-type plants. α-naphthylacetic
acid (α-NAA) treatment was able to rescue the mutated phenotypes occurring in the RNAi plants. Overexpression or suppression of the OsPIN1 expression through a transgenic approach resulted in changes of tiller numbers and shoot/root ratio. Taken together, these data suggest that OsPIN1 plays an important role in auxindependent adventitious root emergence and tillering.

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0743400|
Description = Auxin transport protein REH1|
Version = NM_001054630.1 GI:115448630 GeneID:4330700|
Length = 3075 bp|
Definition = Oryza sativa Japonica Group Os02g0743400, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:32045781..32048855|
CDS = 32046245..32046311,32046396..32046472,32046764..32046921,32047015..32047100,32047215..32047488 ,32047585..32048710|
GCID = <gbrowseImage1>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:32045781..32048855
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaatggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagctgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaa</cdnaseq>|
AA = <aaseq>MITAADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINR FVALFAVPLLSFHFISTNNPYTMNLRFIAADTLQKLMVLAMLTAWSHLSRRGSLEWTI TLFSLSTLPNTLVMGIPLLKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARML ITEQFPDTAANIASIVVDPDVVSLDGRRDAIETETEVKEDGRIHVTVRRSNASRSDIY SRRSMGFSSTTPRPSNLTNAEIYSLQSSRNPTPRGSSFNHTDFYSMVGRSSNFGAADA FGVRTGATPRPSNYEDDASKPKYPLPASNAAPMAGHYPAPNPAVSSAPKGAKKAATNG QAKGEDLHMFVWSSSASPVSDVFGGGAPDYNDAAAVKSPRKMDGAKDREDYVERDDFS FGNRGVMDRDAEAGDEKAAAAAGADPSKAMAAPTAMPPTSVMTRLILIMVWRKLIRNP NTYSSLIGLIWSLVCFRWNFEMPAIVLKSISILSDAGLGMAMFSLGLFMALQPHIIAC GNKVATYAMAVRFLAGPAVMAAASFAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSV HPSILSTAVIFGMLIALPITLVYYILLGL</aaseq>|
DNA = <dnaseqindica>2545..2611#2384..2460#1935..2092#1756..1841#1368..1641#146..1271#gcgcacacacccaatcaaatgctcctcctccgccgcctcctctcgctgagctgagctgagctgtgaaatagtgccaccgagtgagcgctgagctgagctcgaagcaagagaggaagaggaggaggagggaagagggggggcgaagatgattacggcggcggacttctaccacgtgatgacggcgatggtgccgttgtacgtggcgatgatactggcgtacgggtcggtgaagtggtggcgcatcttcacgccggaccagtgctccgggatcaaccgcttcgtggcgctcttcgccgtgccgctgctgtcgttccacttcatctccaccaacaacccgtacacgatgaacctccggttcatcgccgccgacacgctgcagaagctgatggtgctggccatgctcacggcgtggagccacctcagccgccgggggagcctcgagtggaccatcacgctcttctccctctccacgctgcccaacacgctcgtcatggggatccctttgctcaagggcatgtacggggagttctccggcagcctcatggtgcagatcgtcgtgctgcagtgcatcatctggtacacgctcatgctcttcatgttcgagtaccgcggcgcccggatgctcatcaccgagcagttcccggacaccgccgccaacatcgcctccatcgtcgtcgacccggacgtcgtgtcgctggacggcaggagggacgccatcgagacggagacggaggtgaaggaggacggcaggatacacgtcaccgtgcgccgctccaacgcgtctcgctcggacatctactcccgccgctccatgggcttctccagcaccacgccgcggccgagcaacctcaccaacgccgagatctactcgctgcagtcgtcgcggaacccgacgccgaggggttcaagcttcaaccacaccgacttctactccatggtgggccgcagctccaacttcggcgcggccgacgcgttcggcgtccgcaccggcgccacgccgcgcccgtccaactacgaggacgacgcgtccaagcccaagtacccgctcccggcgtcgaatgcggcgcccatggcgggccactacccggcgccgaacccggccgtgtcgtcggcgcccaagggcgccaagaaggcggccacgaacgggcaggccaagggcgaggacctccacatgttcgtctggagctccagcgcgtcgcccgtgtccgacgtcttcggcggcggcgcgccagactacaacgacgccgcggcagtcaagtccccccgcaaaagtagcaatcttttatcttcaccgtgtactatttgcctgatttggggagcatttcttgggctaatttgtactgactcgtatatttcttttacgtcagtggatggagcgaaggacagggaggactacgtggagcgggacgatttcagcttcgggaacaggggcgtcatggacagggacgcggaggcaggggacgagaaggcggcggcggcggcgggcgccgaccccagcaaggccatggcggcgccgacggcgatgccgccgacgagcgtgatgacccgcctcatcctgatcatggtgtggcgcaagctcatccgcaacccgaacacctactccagcctcatcggcctcatctggtccctcgtctgcttcaggtgcgtacaccccgggcttctcgcaaacccaaacccatctgctcgtacgtgctgtggtggtgcggtgtggtggtctggttctgacaaggcgttcgtctcgttttggcgttgcaggtggaacttcgagatgccggccatcgtcctgaaatccatctcgatcctgtcggacgcggggctcggcatggccatgttcagtctcggtcggtattctcttgtttccatgctaacctcacctcacccccggcagtgtcacgagctgttcccctgagtttgcgatctcgccatgcatgcaggtctgttcatggcgctgcagccgcacatcatcgcgtgcgggaacaaggtggcgacgtacgccatggcggtgcggttcctggccgggccggccgtgatggcggcggcgtccttcgccgtcggactccgtggcacgctcctgcacgtcgccattgtccaggtaaaccggaacggaaaagaacagccatacgcacacactgtcacacgcacagtcacacaccgaaaataaaggtctccgtgtgggtgggcatgcaacctaccgcgcgcagccagccagcgtcgttcttttctcccagcccctcgtgctcgttcgcgtctgctttctctcctgccttttgctctccacggccgcggcactttctacgtttggccagcacgaaagccttttgctttggctaacctttttctctctgttgttcttttgtttggtccaaaccgaacgaacgaacaggcagctctgccccagggcattgtccccttcgtcttcgccaaggagtacagcgtgcaccctagcattctcagcacagcgtaagtactagattctctctcagtttgcactctgtcctgaatcgacgggccactgacattttttgcctttgctcctgctcgcagtgtcatctttggcatgctcatcgccttgcctatcaccctcgtctactacatcttgcttgggctgtaatcgagttgcatgcatgtaaattcctgctcctgacaaccagccatgttaagaagaggggagaagaagacagagctggtacactgtttgcaaagtcaggactctttgatttttcttttcttttctgtatttcttgaagtagaatttgggaggagggggattggaagggagtcaaacgagtcaaggggaggacaggatggtagctagcttagctaggacaatggtgagtcacaaaagagcaccaaaagcaagtacaagtacaaagcttggggggacacaggatccagttcaggtcacagaaacggttcggttttgggaggggattgtgggagttttggttggctgcgctgcgctgacccttgtaaaacggacgccgattctgacaagagatcgaccttgttttgttcgtgtcgtggtattgctgactactactttggaatgattaatctcctactactaattactcc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001054630.1 RefSeq:Os02g0743400]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

File:Adventitious roots in OsPIN1 transgenic plants.jpg

2014-06-03T11:39:33Z

Hylwayne: Adventitious roots in OsPIN1 transgenic plants

Adventitious roots in OsPIN1 transgenic plants

File:Phenotypes of OsPIN1 transgenic plants.jpg

2014-06-03T11:38:50Z

Hylwayne: Phenotypes of OsPIN1 transgenic plants

Phenotypes of OsPIN1 transgenic plants

File:OsPIN1 expression in different tissues.jpg

2014-06-03T11:37:45Z

Hylwayne: OsPIN1 expression in different tissues

OsPIN1 expression in different tissues

File:Gene structure and characterization of OsPIN1(2).jpg

2014-06-03T11:34:50Z

Hylwayne: Gene structure and characterization of OsPIN1(2)

Gene structure and characterization of OsPIN1(2)

File:Gene structure and characterization of OsPIN1(1).jpg

2014-06-03T11:33:01Z

Hylwayne: Gene structure and characterization of OsPIN1(1)

Gene structure and characterization of OsPIN1(1)