http://192.168.164.12:81/ricewiki/api.php?action=feedcontributions&feedformat=atom&user=Jieomlong RiceWiki - User contributions [en] 2026-05-27T03:09:09Z User contributions MediaWiki 1.30.0 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=181414 Os10g0127900 2014-06-08T15:01:36Z <p>Jieomlong: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> <br /> ===Expression===<br /> 1.OsFbx352 encodes a F-box domain protein and is suppressed by glucose<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.[[File:1.gif]]<br /> <br /> <br /> Fig. 1. OsFbx352 contains a conserved F-box domain.(A) Alignment of the conserved F-box domain in OsFbx352 withother representative F-box-containing proteins: blue indicates residues identical to the OsFbx352 sequence; other colours indicate similar amino acids. The sequence at the bottom is the consensus sequence. (B)Phylogenetic tree analysis of the conserved F-box domain in OsFbx352 with other known F-box proteins from diverse organisms. Alignment and phylogenetic<br /> analysis were performed using clustal x 2 and mega 5, respectively. Approximately 40 amino acids that constitute the<br /> F-box motif in OsFBX352 were aligned with comparable regions from reported F-box proteins in humans (SKP2, BAB87202.1), yeast (CDC4, NP_116585.1), Arabidopsis (COI1, NP_565919; SON1, NP_179323; UFO, NP_564368.1; TIR1, NP_567135.1), and rice (OsMAIF, NP_001047698.1; OsDRF, NP_001052823.1).<br /> <br /> 2.Generation of transgenic rice plants with altered expression of OsFbx352<br /> <br /> To characterize the function of OsFbx352, transgenic rice plants were generated by constructing a RNAi construct with part of the coding sequence fragment to knock down OsFbx352 expression, as well as an overexpression construct with the OsFbx352 full-length open reading frame driven by a maize ubiquitin promoter. Both constructs were introduced into rice calluses via Agrobacterium-mediated transformation. Several transgenic lines were obtained and subsequently confirmed<br /> by qRT-PCR. The expression of OsFbx352 in the overexpression seeds was at least 11-fold higher than that in WT seeds, while OsFbx352 transcripts in seeds of the knockdown lines were reduced by approximately 70% compared with WT seeds (Fig. 2). Among the transgenic lines, two overexpression lines (OE10, OE24) and two knockdown lines (Ri15, Ri18) were selected to study the physiological function of OsFbx352 during seed germination.<br /> [[File:2.jpg]]<br /> <br /> Fig. 2. Quantitative real-time PCR analysis of OsFbx352 expression in wild-type (WT), overexpression (OE10, OE11, OE13, OE14, and OE24), and RNAi (Ri6, Ri15, and Ri18) plants. Data are mean ± SE for three replicates. Values above columns are the changes in gene expression relative to the WT.<br /> <br /> 3.Germination of transgenic seeds displayed different sensitivity to ABA<br /> Given that seed germination is sensitive to ABA and expression of OsFbx352 was strongly induced by ABA, the role of OsFbx352 played in seed germination was evaluated. There were no differences in seed germination among WT and transgenic lines in the control medium, and both WT and transgenic seeds were fully<br /> germinated after 4 days of incubation in the control medium (Fig. 3A). Germination of WT and RNAi4 seeds was delayed when ABA was present in the incubation medium, while germination of seeds with overexpressing OsFbx352 was relatively insensitive to ABA (Fig. 3B). In addition, the inhibitory effect of ABA<br /> on germination of RNAi seeds was more evident than that of WT seeds (Fig. 3B). For instance, both WT and transgenic seeds were germinated after 4 days in the control medium, while the germination rate for WT and RNAi seeds was reduced to approximately 80 and 60%, respectively, after the same period incubation in the<br /> presence of ABA (Fig. 3A, 3B). Moreover, the inhibitory effect of ABA on seed germination of WT and RNAi seeds was increased with an increase in ABA concentrations (Fig. 3C). In addition toseed germination, overexpression of OsFbx352 also rendered the seedlings less sensitive to ABA, as evidenced by longer shoot length of overexpression lines than the WT in the presence of ABA while suppression of OsFbx352 led to a shorter shoot length than the WT.[[File:3.jpg]]<br /> <br /> Fig. 3. OsFbx352-overexpressing seeds exhibited decreased sensitivity to exogenous abscisic acid (ABA). (A, B) Seeds of wild-type (WT), OE10, OE24, Ri15, and Ri18 lines were germinated in control medium (A) or 4 μM ABA (B) for 4 days. (C) Seed germination rate for WT and transgenic rice seeds after incubation in without and with different concentrations of ABA for 4 days. Data are mean ± SE for three replicates with each replicate containing 30 seeds. Statistically significant difference from the wild type within a treatment: *, P &lt; 0.05; **, P &lt; 0.01 (this figure is available in colour at JXB online).<br /> <br /> ===Evolution===<br /> F-box proteins comprise one of the large protein families in plants. There are more than 692 and 779 F-box genes in the A. thaliana and rice genomes, respectively .Previous studies demonstrated that F-box proteins are involved in the regulation of several biological processes, including circadian clock, self-incompatibility, photomorphogenesis, floral meristem, phytohormonal signalling, and responses to abiotic stresses ; Dreher and Callis, 2007). For instance, the F-box protein TIR1/AFB can bind to the transcriptional repressor protein Aux/IAA, thus promoting their proteasomal degradation and activating auxin-induced transcription. The involvement of F-box proteins in response to both biotic and abiotic stresses has also been reported. For example, four F-box proteins from different species (DOR, OsDRF1, PvFBS1, and MAIF1) have been shown to act as key regulators in responses to biotic and abiotic stress; reported that an F-box protein, ZTL, is a major factor to control the period of circadian and early photomorphogenesis by regulating PRR5 for proteasome-dependent degradation. As a primary sugar, glucose modulates many important physiological processes ranging from seed germination to seedling development.<br /> <br /> It has been well documented that the inhibitory effect of sugaron seed germination is due to the increase in the active endogenous ABA level (Arenas-Huertero et . For example, Zhu et al. (2009) reported that a higher ABA content in imbibed seeds may account for the glucose-induced delay of rice seed germination. The present results showing that ABA contents were markedly increased in imbibed seeds of both WT and transgenic lines with altered expression of OsFbx352 when challenged with glucose are in line with those findings reported in the literature. In addition, this study found that the glucose-induced increase in ABA content in the<br /> OsFbx352-overexpressing seeds was lower than in WT seeds, while it was higher in the two RNAi seeds than in WT seeds. These results suggest that overexpression of OsFbx352 disrupts glucose-induced ABA metabolism, thus rendering the germination of overexpressing seeds less sensitive to glucose.<br /> <br /> ==Labs working on this gene==<br /> State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, the Chinese Academy of Sciences,Beijing 100093 PR China.<br /> <br /> ==References==<br /> 1. Song, S., X. Dai, and W.H. Zhang, A rice F-box gene, OsFbx352, is involved in glucose-delayed seed germination in rice. J Exp Bot, 2012. 63(15): p. 5559-68.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaa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/> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=181413 Os10g0127900 2014-06-08T15:00:49Z <p>Jieomlong: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> <br /> ===Expression===<br /> 1.OsFbx352 encodes a F-box domain protein and is suppressed by glucose<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.[[File:1.gif]]<br /> <br /> <br /> Fig. 1. OsFbx352 contains a conserved F-box domain.(A) Alignment of the conserved F-box domain in OsFbx352 withother representative F-box-containing proteins: blue indicates residues identical to the OsFbx352 sequence; other colours indicate similar amino acids. The sequence at the bottom is the consensus sequence. (B)Phylogenetic tree analysis of the conserved F-box domain in OsFbx352 with other known F-box proteins from diverse organisms. Alignment and phylogenetic<br /> analysis were performed using clustal x 2 and mega 5, respectively. Approximately 40 amino acids that constitute the<br /> F-box motif in OsFBX352 were aligned with comparable regions from reported F-box proteins in humans (SKP2, BAB87202.1), yeast (CDC4, NP_116585.1), Arabidopsis (COI1, NP_565919; SON1, NP_179323; UFO, NP_564368.1; TIR1, NP_567135.1), and rice (OsMAIF, NP_001047698.1; OsDRF, NP_001052823.1).<br /> <br /> 2.Generation of transgenic rice plants with altered expression of OsFbx352<br /> <br /> To characterize the function of OsFbx352, transgenic rice plants were generated by constructing a RNAi construct with part of the coding sequence fragment to knock down OsFbx352 expression, as well as an overexpression construct with the OsFbx352 full-length open reading frame driven by a maize ubiquitin promoter. Both constructs were introduced into rice calluses via Agrobacterium-mediated transformation. Several transgenic lines were obtained and subsequently confirmed<br /> by qRT-PCR. The expression of OsFbx352 in the overexpression seeds was at least 11-fold higher than that in WT seeds, while OsFbx352 transcripts in seeds of the knockdown lines were reduced by approximately 70% compared with WT seeds (Fig. 2). Among the transgenic lines, two overexpression lines (OE10, OE24) and two knockdown lines (Ri15, Ri18) were selected to study the physiological function of OsFbx352 during seed germination.<br /> [[File:2.jpg]]<br /> Fig. 2. Quantitative real-time PCR analysis of OsFbx352 expression in wild-type (WT), overexpression (OE10, OE11, OE13, OE14, and OE24), and RNAi (Ri6, Ri15, and Ri18) plants. Data are mean ± SE for three replicates. Values above columns are the changes in gene expression relative to the WT.<br /> <br /> 3.Germination of transgenic seeds displayed different sensitivity to ABA<br /> Given that seed germination is sensitive to ABA and expression of OsFbx352 was strongly induced by ABA, the role of OsFbx352 played in seed germination was evaluated. There were no differences in seed germination among WT and transgenic lines in the control medium, and both WT and transgenic seeds were fully<br /> germinated after 4 days of incubation in the control medium (Fig. 3A). Germination of WT and RNAi4 seeds was delayed when ABA was present in the incubation medium, while germination of seeds with overexpressing OsFbx352 was relatively insensitive to ABA (Fig. 3B). In addition, the inhibitory effect of ABA<br /> on germination of RNAi seeds was more evident than that of WT seeds (Fig. 3B). For instance, both WT and transgenic seeds were germinated after 4 days in the control medium, while the germination rate for WT and RNAi seeds was reduced to approximately 80 and 60%, respectively, after the same period incubation in the<br /> presence of ABA (Fig. 3A, 3B). Moreover, the inhibitory effect of ABA on seed germination of WT and RNAi seeds was increased with an increase in ABA concentrations (Fig. 3C). In addition toseed germination, overexpression of OsFbx352 also rendered the seedlings less sensitive to ABA, as evidenced by longer shoot length of overexpression lines than the WT in the presence of ABA while suppression of OsFbx352 led to a shorter shoot length than the WT.[[File:3.jpg]]<br /> <br /> Fig. 3. OsFbx352-overexpressing seeds exhibited decreased sensitivity to exogenous abscisic acid (ABA). (A, B) Seeds of wild-type (WT), OE10, OE24, Ri15, and Ri18 lines were germinated in control medium (A) or 4 μM ABA (B) for 4 days. (C) Seed germination rate for WT and transgenic rice seeds after incubation in without and with different concentrations of ABA for 4 days. Data are mean ± SE for three replicates with each replicate containing 30 seeds. Statistically significant difference from the wild type within a treatment: *, P &lt; 0.05; **, P &lt; 0.01 (this figure is available in colour at JXB online).<br /> <br /> ===Evolution===<br /> F-box proteins comprise one of the large protein families in plants. There are more than 692 and 779 F-box genes in the A. thaliana and rice genomes, respectively .Previous studies demonstrated that F-box proteins are involved in the regulation of several biological processes, including circadian clock, self-incompatibility, photomorphogenesis, floral meristem, phytohormonal signalling, and responses to abiotic stresses ; Dreher and Callis, 2007). For instance, the F-box protein TIR1/AFB can bind to the transcriptional repressor protein Aux/IAA, thus promoting their proteasomal degradation and activating auxin-induced transcription. The involvement of F-box proteins in response to both biotic and abiotic stresses has also been reported. For example, four F-box proteins from different species (DOR, OsDRF1, PvFBS1, and MAIF1) have been shown to act as key regulators in responses to biotic and abiotic stress; reported that an F-box protein, ZTL, is a major factor to control the period of circadian and early photomorphogenesis by regulating PRR5 for proteasome-dependent degradation. As a primary sugar, glucose modulates many important physiological processes ranging from seed germination to seedling development.<br /> <br /> It has been well documented that the inhibitory effect of sugaron seed germination is due to the increase in the active endogenous ABA level (Arenas-Huertero et . For example, Zhu et al. (2009) reported that a higher ABA content in imbibed seeds may account for the glucose-induced delay of rice seed germination. The present results showing that ABA contents were markedly increased in imbibed seeds of both WT and transgenic lines with altered expression of OsFbx352 when challenged with glucose are in line with those findings reported in the literature. In addition, this study found that the glucose-induced increase in ABA content in the<br /> OsFbx352-overexpressing seeds was lower than in WT seeds, while it was higher in the two RNAi seeds than in WT seeds. These results suggest that overexpression of OsFbx352 disrupts glucose-induced ABA metabolism, thus rendering the germination of overexpressing seeds less sensitive to glucose.<br /> <br /> ==Labs working on this gene==<br /> State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, the Chinese Academy of Sciences,Beijing 100093 PR China.<br /> <br /> ==References==<br /> 1. Song, S., X. Dai, and W.H. Zhang, A rice F-box gene, OsFbx352, is involved in glucose-delayed seed germination in rice. J Exp Bot, 2012. 63(15): p. 5559-68.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaa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/> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=181412 Os10g0127900 2014-06-08T15:00:03Z <p>Jieomlong: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> <br /> ===Expression===<br /> 1.OsFbx352 encodes a F-box domain protein and is suppressed by glucose<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.[[File:1.gif]]<br /> <br /> <br /> Fig. 1. OsFbx352 contains a conserved F-box domain.(A) Alignment of the conserved F-box domain in OsFbx352 withother representative F-box-containing proteins: blue indicates residues identical to the OsFbx352 sequence; other colours indicate similar amino acids. The sequence at the bottom is the consensus sequence. (B)Phylogenetic tree analysis of the conserved F-box domain in OsFbx352 with other known F-box proteins from diverse organisms. Alignment and phylogenetic<br /> analysis were performed using clustal x 2 and mega 5, respectively. Approximately 40 amino acids that constitute the<br /> F-box motif in OsFBX352 were aligned with comparable regions from reported F-box proteins in humans (SKP2, BAB87202.1), yeast (CDC4, NP_116585.1), Arabidopsis (COI1, NP_565919; SON1, NP_179323; UFO, NP_564368.1; TIR1, NP_567135.1), and rice (OsMAIF, NP_001047698.1; OsDRF, NP_001052823.1).<br /> <br /> 2.Generation of transgenic rice plants with altered expression of OsFbx352<br /> <br /> To characterize the function of OsFbx352, transgenic rice plants were generated by constructing a RNAi construct with part of the coding sequence fragment to knock down OsFbx352 expression, as well as an overexpression construct with the OsFbx352 full-length open reading frame driven by a maize ubiquitin promoter. Both constructs were introduced into rice calluses via Agrobacterium-mediated transformation. Several transgenic lines were obtained and subsequently confirmed<br /> by qRT-PCR. The expression of OsFbx352 in the overexpression seeds was at least 11-fold higher than that in WT seeds, while OsFbx352 transcripts in seeds of the knockdown lines were reduced by approximately 70% compared with WT seeds (Fig. 2). Among the transgenic lines, two overexpression lines (OE10, OE24) and two knockdown lines (Ri15, Ri18) were selected to study the physiological function of OsFbx352 during seed germination.<br /> [[File:2.jpg]]<br /> <br /> Fig. 2. Quantitative real-time PCR analysis of OsFbx352 expression in wild-type (WT), overexpression (OE10, OE11, OE13, OE14, and OE24), and RNAi (Ri6, Ri15, and Ri18) plants. Data are mean ± SE for three replicates. Values above columns are the changes in gene expression relative to the WT.<br /> <br /> 3.Germination of transgenic seeds displayed different sensitivity to ABA<br /> Given that seed germination is sensitive to ABA and expression of OsFbx352 was strongly induced by ABA, the role of OsFbx352 played in seed germination was evaluated. There were no differences in seed germination among WT and transgenic lines in the control medium, and both WT and transgenic seeds were fully<br /> germinated after 4 days of incubation in the control medium (Fig. 3A). Germination of WT and RNAi4 seeds was delayed when ABA was present in the incubation medium, while germination of seeds with overexpressing OsFbx352 was relatively insensitive to ABA (Fig. 3B). In addition, the inhibitory effect of ABA<br /> on germination of RNAi seeds was more evident than that of WT seeds (Fig. 3B). For instance, both WT and transgenic seeds were germinated after 4 days in the control medium, while the germination rate for WT and RNAi seeds was reduced to approximately 80 and 60%, respectively, after the same period incubation in the<br /> presence of ABA (Fig. 3A, 3B). Moreover, the inhibitory effect of ABA on seed germination of WT and RNAi seeds was increased with an increase in ABA concentrations (Fig. 3C). In addition toseed germination, overexpression of OsFbx352 also rendered the seedlings less sensitive to ABA, as evidenced by longer shoot length of overexpression lines than the WT in the presence of ABA while suppression of OsFbx352 led to a shorter shoot length than the WT.[[File:3.jpg]]<br /> <br /> Fig. 3. OsFbx352-overexpressing seeds exhibited decreased sensitivity to exogenous abscisic acid (ABA). (A, B) Seeds of wild-type (WT), OE10, OE24, Ri15, and Ri18 lines were germinated in control medium (A) or 4 μM ABA (B) for 4 days. (C) Seed germination rate for WT and transgenic rice seeds after incubation in without and with different concentrations of ABA for 4 days. Data are mean ± SE for three replicates with each replicate containing 30 seeds. Statistically significant difference from the wild type within a treatment: *, P &lt; 0.05; **, P &lt; 0.01 (this figure is available in colour at JXB online).<br /> <br /> ===Evolution===<br /> F-box proteins comprise one of the large protein families in plants. There are more than 692 and 779 F-box genes in the A. thaliana and rice genomes, respectively .Previous studies demonstrated that F-box proteins are involved in the regulation of several biological processes, including circadian clock, self-incompatibility, photomorphogenesis, floral meristem, phytohormonal signalling, and responses to abiotic stresses ; Dreher and Callis, 2007). For instance, the F-box protein TIR1/AFB can bind to the transcriptional repressor protein Aux/IAA, thus promoting their proteasomal degradation and activating auxin-induced transcription. The involvement of F-box proteins in response to both biotic and abiotic stresses has also been reported. For example, four F-box proteins from different species (DOR, OsDRF1, PvFBS1, and MAIF1) have been shown to act as key regulators in responses to biotic and abiotic stress; reported that an F-box protein, ZTL, is a major factor to control the period of circadian and early photomorphogenesis by regulating PRR5 for proteasome-dependent degradation. As a primary sugar, glucose modulates many important physiological processes ranging from seed germination to seedling development.<br /> <br /> It has been well documented that the inhibitory effect of sugaron seed germination is due to the increase in the active endogenous ABA level (Arenas-Huertero et . For example, Zhu et al. (2009) reported that a higher ABA content in imbibed seeds may account for the glucose-induced delay of rice seed germination. The present results showing that ABA contents were markedly increased in imbibed seeds of both WT and transgenic lines with altered expression of OsFbx352 when challenged with glucose are in line with those findings reported in the literature. In addition, this study found that the glucose-induced increase in ABA content in the<br /> OsFbx352-overexpressing seeds was lower than in WT seeds, while it was higher in the two RNAi seeds than in WT seeds. These results suggest that overexpression of OsFbx352 disrupts glucose-induced ABA metabolism, thus rendering the germination of overexpressing seeds less sensitive to glucose.<br /> <br /> ==Labs working on this gene==<br /> State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, the Chinese Academy of Sciences,Beijing 100093 PR China.<br /> <br /> ==References==<br /> 1. Song, S., X. Dai, and W.H. Zhang, A rice F-box gene, OsFbx352, is involved in glucose-delayed seed germination in rice. J Exp Bot, 2012. 63(15): p. 5559-68.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaa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/> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=181411 Os10g0127900 2014-06-08T14:58:42Z <p>Jieomlong: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> <br /> ===Expression===<br /> 1.OsFbx352 encodes a F-box domain protein and is suppressed by glucose<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.[[File:1.gif]]<br /> <br /> <br /> Fig. 1. OsFbx352 contains a conserved F-box domain.(A) Alignment of the conserved F-box domain in OsFbx352 withother representative F-box-containing proteins: blue indicates residues identical to the OsFbx352 sequence; other colours indicate similar amino acids. The sequence at the bottom is the consensus sequence. (B)Phylogenetic tree analysis of the conserved F-box domain in OsFbx352 with other known F-box proteins from diverse organisms. Alignment and phylogenetic<br /> analysis were performed using clustal x 2 and mega 5, respectively. Approximately 40 amino acids that constitute the<br /> F-box motif in OsFBX352 were aligned with comparable regions from reported F-box proteins in humans (SKP2, BAB87202.1), yeast (CDC4, NP_116585.1), Arabidopsis (COI1, NP_565919; SON1, NP_179323; UFO, NP_564368.1; TIR1, NP_567135.1), and rice (OsMAIF, NP_001047698.1; OsDRF, NP_001052823.1).<br /> <br /> 2.Generation of transgenic rice plants with altered expression of OsFbx352<br /> <br /> To characterize the function of OsFbx352, transgenic rice plants were generated by constructing a RNAi construct with part of the coding sequence fragment to knock down OsFbx352 expression, as well as an overexpression construct with the OsFbx352 full-length open reading frame driven by a maize ubiquitin promoter. Both constructs were introduced into rice calluses via Agrobacterium-mediated transformation. Several transgenic lines were obtained and subsequently confirmed<br /> by qRT-PCR. The expression of OsFbx352 in the overexpression seeds was at least 11-fold higher than that in WT seeds, while OsFbx352 transcripts in seeds of the knockdown lines were reduced by approximately 70% compared with WT seeds (Fig. 2). Among the transgenic lines, two overexpression lines (OE10, OE24) and two knockdown lines (Ri15, Ri18) were selected to study the physiological function of OsFbx352 during seed germination.<br /> [[File:2.jpg]]<br /> <br /> <br /> Fig. 2. Quantitative real-time PCR analysis of OsFbx352 expression in wild-type (WT), overexpression (OE10, OE11, OE13, OE14, and OE24), and RNAi (Ri6, Ri15, and Ri18) plants. Data are mean ± SE for three replicates. Values above columns are the changes in gene expression relative to the WT.<br /> <br /> 3.Germination of transgenic seeds displayed different sensitivity to ABA<br /> Given that seed germination is sensitive to ABA and expression of OsFbx352 was strongly induced by ABA, the role of OsFbx352 played in seed germination was evaluated. There were no differences in seed germination among WT and transgenic lines in the control medium, and both WT and transgenic seeds were fully<br /> germinated after 4 days of incubation in the control medium (Fig. 3A). Germination of WT and RNAi4 seeds was delayed when ABA was present in the incubation medium, while germination of seeds with overexpressing OsFbx352 was relatively insensitive to ABA (Fig. 3B). In addition, the inhibitory effect of ABA<br /> on germination of RNAi seeds was more evident than that of WT seeds (Fig. 3B). For instance, both WT and transgenic seeds were germinated after 4 days in the control medium, while the germination rate for WT and RNAi seeds was reduced to approximately 80 and 60%, respectively, after the same period incubation in the<br /> presence of ABA (Fig. 3A, 3B). Moreover, the inhibitory effect of ABA on seed germination of WT and RNAi seeds was increased with an increase in ABA concentrations (Fig. 3C). In addition toseed germination, overexpression of OsFbx352 also rendered the seedlings less sensitive to ABA, as evidenced by longer shoot length of overexpression lines than the WT in the presence of ABA while suppression of OsFbx352 led to a shorter shoot length than the WT.[[File:3.jpg]]<br /> Fig. 3. OsFbx352-overexpressing seeds exhibited decreased sensitivity to exogenous abscisic acid (ABA). (A, B) Seeds of wild-type (WT), OE10, OE24, Ri15, and Ri18 lines were germinated in control medium (A) or 4 μM ABA (B) for 4 days. (C) Seed germination rate for WT and transgenic rice seeds after incubation in without and with different concentrations of ABA for 4 days. Data are mean ± SE for three replicates with each replicate containing 30 seeds. Statistically significant difference from the wild type within a treatment: *, P &lt; 0.05; **, P &lt; 0.01 (this figure is available in colour at JXB online).<br /> <br /> ===Evolution===<br /> F-box proteins comprise one of the large protein families in plants. There are more than 692 and 779 F-box genes in the A. thaliana and rice genomes, respectively .Previous studies demonstrated that F-box proteins are involved in the regulation of several biological processes, including circadian clock, self-incompatibility, photomorphogenesis, floral meristem, phytohormonal signalling, and responses to abiotic stresses ; Dreher and Callis, 2007). For instance, the F-box protein TIR1/AFB can bind to the transcriptional repressor protein Aux/IAA, thus promoting their proteasomal degradation and activating auxin-induced transcription. The involvement of F-box proteins in response to both biotic and abiotic stresses has also been reported. For example, four F-box proteins from different species (DOR, OsDRF1, PvFBS1, and MAIF1) have been shown to act as key regulators in responses to biotic and abiotic stress; reported that an F-box protein, ZTL, is a major factor to control the period of circadian and early photomorphogenesis by regulating PRR5 for proteasome-dependent degradation. As a primary sugar, glucose modulates many important physiological processes ranging from seed germination to seedling development.<br /> <br /> It has been well documented that the inhibitory effect of sugaron seed germination is due to the increase in the active endogenous ABA level (Arenas-Huertero et . For example, Zhu et al. (2009) reported that a higher ABA content in imbibed seeds may account for the glucose-induced delay of rice seed germination. The present results showing that ABA contents were markedly increased in imbibed seeds of both WT and transgenic lines with altered expression of OsFbx352 when challenged with glucose are in line with those findings reported in the literature. In addition, this study found that the glucose-induced increase in ABA content in the<br /> OsFbx352-overexpressing seeds was lower than in WT seeds, while it was higher in the two RNAi seeds than in WT seeds. These results suggest that overexpression of OsFbx352 disrupts glucose-induced ABA metabolism, thus rendering the germination of overexpressing seeds less sensitive to glucose.<br /> <br /> ==Labs working on this gene==<br /> State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, the Chinese Academy of Sciences,Beijing 100093 PR China.<br /> <br /> ==References==<br /> 1. Song, S., X. Dai, and W.H. Zhang, A rice F-box gene, OsFbx352, is involved in glucose-delayed seed germination in rice. J Exp Bot, 2012. 63(15): p. 5559-68.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaa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/> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=181407 Os10g0127900 2014-06-08T14:57:25Z <p>Jieomlong: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> <br /> ===Expression===<br /> 1.OsFbx352 encodes a F-box domain protein and is suppressed by glucose<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.[[File:1.gif]]<br /> <br /> <br /> Fig. 1. OsFbx352 contains a conserved F-box domain.(A) Alignment of the conserved F-box domain in OsFbx352 withother representative F-box-containing proteins: blue indicates residues identical to the OsFbx352 sequence; other colours indicate similar amino acids. The sequence at the bottom is the consensus sequence. (B)Phylogenetic tree analysis of the conserved F-box domain in OsFbx352 with other known F-box proteins from diverse organisms. Alignment and phylogenetic<br /> analysis were performed using clustal x 2 and mega 5, respectively. Approximately 40 amino acids that constitute the<br /> F-box motif in OsFBX352 were aligned with comparable regions from reported F-box proteins in humans (SKP2, BAB87202.1), yeast (CDC4, NP_116585.1), Arabidopsis (COI1, NP_565919; SON1, NP_179323; UFO, NP_564368.1; TIR1, NP_567135.1), and rice (OsMAIF, NP_001047698.1; OsDRF, NP_001052823.1).<br /> <br /> 2.Generation of transgenic rice plants with altered expression of OsFbx352<br /> <br /> To characterize the function of OsFbx352, transgenic rice plants were generated by constructing a RNAi construct with part of the coding sequence fragment to knock down OsFbx352 expression, as well as an overexpression construct with the OsFbx352 full-length open reading frame driven by a maize ubiquitin promoter. Both constructs were introduced into rice calluses via Agrobacterium-mediated transformation. Several transgenic lines were obtained and subsequently confirmed<br /> by qRT-PCR. The expression of OsFbx352 in the overexpression seeds was at least 11-fold higher than that in WT seeds, while OsFbx352 transcripts in seeds of the knockdown lines were reduced by approximately 70% compared with WT seeds (Fig. 2). Among the transgenic lines, two overexpression lines (OE10, OE24) and two knockdown lines (Ri15, Ri18) were selected to study the physiological function of OsFbx352 during seed germination.<br /> [[File:2.jpg]]<br /> <br /> <br /> Fig. 2. Quantitative real-time PCR analysis of OsFbx352 expression in wild-type (WT), overexpression (OE10, OE11, OE13, OE14, and OE24), and RNAi (Ri6, Ri15, and Ri18) plants. Data are mean ± SE for three replicates. Values above columns are the changes in gene expression relative to the WT.<br /> <br /> 3.Germination of transgenic seeds displayed different sensitivity to ABA<br /> Given that seed germination is sensitive to ABA and expression of OsFbx352 was strongly induced by ABA, the role of OsFbx352 played in seed germination was evaluated. There were no differences in seed germination among WT and transgenic lines in the control medium, and both WT and transgenic seeds were fully<br /> germinated after 4 days of incubation in the control medium (Fig. 3A). Germination of WT and RNAi4 seeds was delayed when ABA was present in the incubation medium, while germination of seeds with overexpressing OsFbx352 was relatively insensitive to ABA (Fig. 3B). In addition, the inhibitory effect of ABA<br /> on germination of RNAi seeds was more evident than that of WT seeds (Fig. 3B). For instance, both WT and transgenic seeds were germinated after 4 days in the control medium, while the germination rate for WT and RNAi seeds was reduced to approximately 80 and 60%, respectively, after the same period incubation in the<br /> presence of ABA (Fig. 3A, 3B). Moreover, the inhibitory effect of ABA on seed germination of WT and RNAi seeds was increased with an increase in ABA concentrations (Fig. 3C). In addition toseed germination, overexpression of OsFbx352 also rendered the seedlings less sensitive to ABA, as evidenced by longer shoot length of overexpression lines than the WT in the presence of ABA while suppression of OsFbx352 led to a shorter shoot length than the WT.[[File:3.jpg]]<br /> <br /> Fig. 3. OsFbx352-overexpressing seeds exhibited decreased sensitivity to exogenous abscisic acid (ABA). (A, B) Seeds of wild-type (WT), OE10, OE24, Ri15, and Ri18 lines were germinated in control medium (A) or 4 μM ABA (B) for 4 days. (C) Seed germination rate for WT and transgenic rice seeds after incubation in without and with different concentrations of ABA for 4 days. Data are mean ± SE for three replicates with each replicate containing 30 seeds. Statistically significant difference from the wild type within a treatment: *, P &lt; 0.05; **, P &lt; 0.01 (this figure is available in colour at JXB online).<br /> <br /> ===Evolution===<br /> F-box proteins comprise one of the large protein families in plants. There are more than 692 and 779 F-box genes in the A. thaliana and rice genomes, respectively .Previous studies demonstrated that F-box proteins are involved in the regulation of several biological processes, including circadian clock, self-incompatibility, photomorphogenesis, floral meristem, phytohormonal signalling, and responses to abiotic stresses ; Dreher and Callis, 2007). For instance, the F-box protein TIR1/AFB can bind to the transcriptional repressor protein Aux/IAA, thus promoting their proteasomal degradation and activating auxin-induced transcription. The involvement of F-box proteins in response to both biotic and abiotic stresses has also been reported. For example, four F-box proteins from different species (DOR, OsDRF1, PvFBS1, and MAIF1) have been shown to act as key regulators in responses to biotic and abiotic stress; reported that an F-box protein, ZTL, is a major factor to control the period of circadian and early photomorphogenesis by regulating PRR5 for proteasome-dependent degradation. As a primary sugar, glucose modulates many important physiological processes ranging from seed germination to seedling development.<br /> <br /> It has been well documented that the inhibitory effect of sugaron seed germination is due to the increase in the active endogenous ABA level (Arenas-Huertero et . For example, Zhu et al. (2009) reported that a higher ABA content in imbibed seeds may account for the glucose-induced delay of rice seed germination. The present results showing that ABA contents were markedly increased in imbibed seeds of both WT and transgenic lines with altered expression of OsFbx352 when challenged with glucose are in line with those findings reported in the literature. In addition, this study found that the glucose-induced increase in ABA content in the<br /> OsFbx352-overexpressing seeds was lower than in WT seeds, while it was higher in the two RNAi seeds than in WT seeds. These results suggest that overexpression of OsFbx352 disrupts glucose-induced ABA metabolism, thus rendering the germination of overexpressing seeds less sensitive to glucose.<br /> <br /> ==Labs working on this gene==<br /> State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, the Chinese Academy of Sciences,Beijing 100093 PR China.<br /> <br /> ==References==<br /> 1. Song, S., X. Dai, and W.H. Zhang, A rice F-box gene, OsFbx352, is involved in glucose-delayed seed germination in rice. J Exp Bot, 2012. 63(15): p. 5559-68.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaactttcaagtccaaatttaaaactctcagttcatcttttaaaattttaattcaaccagtctgttcgttattttgctataaataatcacgcttatgctaatctaatcgcgtttagcgctaagcgaggggaggggcgcgtctgcgtgtggcatggcgccgtgcgcgcgagttactgtagcagcgcggatgcgcgagttacgagagaagcggaaaactgaaaacgcgcgagatctcgcgtattactccttgtcacggacagtttgagagcgggaggcctcctcccaatctccccccttccccttccatagttccatggtggcgctcttcagacgcgagcgcgaggagcaccgggtccgcttgccggtggccggcctgactcgttagccgccgtcccgtccggggcgaaaggaagcatacctcgctcactccatttggaggtaatggcttcacccaatctttcttgaatcaccattcctctctggatggatgttcttcaccaaacaatgctgtattgccgctcaatgtagcatcgtttcttgcgctaagatcttgaatcttgcttggagttgagagctggggattcatggtttattgttccgttactgtgcagcttcccccatccgcacgcgcaccagctgttcgacgaaatgccaccgcgcgctctgcccccagtggaggacgacgccggcagggactggctcggtgacctccccgaggaggtcctccaccacatcatgtccttcctagacgcgcgccaggccgtgcgaacctgtgtgctgtcgcggcggtggcgcaacctctggcgtaccgtgccttgcatcaacgctgacttcgatgagtttgatctcgtcttttaccaaggggacgacgaggactatgatgacgtgctggcgttcaaaaggtttgttaaccgtctgctggagctccgcgatcccaccgccatgatagacaccttctggcttaggtacaccaccagaccagaaggaaacacgaattccaatgaagatgcctacgggtggattagccacgctttgcagaagcaggcccgggttttagaggttgtcgtgttctgctgcttatttgagctggaccactcggtgtttacttcctgctacctgagaagaattgcgttctccggtattgttttatgcaagggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaaggtatgcatatttctattacatagtatgcatctttatttaggtgtcaatttgaaaaacttaaagttaagcatggtgttgtttcttcttgctacttcataattgcaattgtttaattacctgtacctgcaaaacttatgggcattgtttggataatgggatagggaaatgatttcccaactatcaaaaggtatctttaaatcctaaccatttattctccctcttccatttaatcctaacccttcattcatttcctaatcccaatcccacccttcatttcccattatccaaacacacccatgattcaagtgatcattttgtcgtgaaatcatgatgtgttgatacgatgaccactgatgttatcaaccactcaatgcaatatagttcatccactccagccattttttgtacacaaatcaatccgttgaacacttgtcctgctgtttctttgttggtagttttatctcaaaatgaaactggatcatttctcatgtgaggactagaatgttctagtctggatgcaatatcagggctgccaaccaaatgtgcatcttgtttgacatctgactgattattttagttgacatcagttcacatcttaccttactttttgaaagagggggttagcggtgagctggtgattgtaggttctagacccaaccctagtccagatttttcttggctgcagacatgtatgcattttatttctaagatatttatagaaaacaatattgcaattgcatatgcggccatttggtattgtgtaatcatgcatgaaatcgttggtttccggattaattatttattgcattttatattttagacagtatgttgaattgggatatgatgcaacgcacagacattatgcatttatgctacataaatttgaaaattaagagacattcgaaggcaatatgcaaacagaaagtgcttcaggattagttgaaaatctcacttctagtgcttcatcatgaaatatttgtactgttctttttactagtttttgttacatgacttgcatggaaagtatcctagcagtaagttaacatgcctgagactcgcattgaaaatatgaacatgtataatattgtttagtctggtcttttgttgacataatttcagtttctcctgtggtcttttaccatccgttatcctaaagttgaagctattatacatgcactccaaatagaattctttatcacatattgttaatgctctcaatttagttgccatattttctgattatattcatttgaatctttcgaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaaggtaaatgctttcgtccaaatgtgcactgatcctacttgattgctttttacacaagagttttcacatcgggatatagatcacctgtttttgttgctgtagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatgaaatggcaaaggatcacgaattagtttgccatcccattgaagaaaatcactctctttcgcctgtcggctacatctgctggttacagtagagtgttcagtgatcttgtttggtgctttctgctatgacgtgaaggtgagtagaaacaattgcatcttgccttgcatgtaaggtcagtggtattgtacttgtttgctgcgatcacatgatgatttgtagcaataatgatgctccaagaaaaatactgtatggctatggcgtctcctgatacagcctgccactgctttctcaacttggctggtctccggtgaaacgtattgtatttgtctctctcctaatataatcccatgttatagtgttgt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:2.jpg&diff=181404 File:2.jpg 2014-06-08T14:54:10Z <p>Jieomlong: uploaded a new version of &amp;quot;File:2.jpg&amp;quot;: Reverted to version as of 02:46, 8 June 2014</p> <hr /> <div>Gross morphology of wildtype (left) and gid1-1 (right) plants. Scale bar, 10 cm. Inset: higher magnification of gid1-1. Scale bar, 1 cm</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0853400&diff=181208 Os01g0853400 2014-06-08T12:54:48Z <p>Jieomlong: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> The F-box protein coronatine insensitive 1 (COI1),（The rice genome contains two closely related COI1genes, OsCOI1a(Os01g0853400) and OsCOI1b(Os05g0449500), which share 83% and 82% sequence identity at the DNA and protein levels, respectively.） a component of the SCF E3 ubiquitin ligase, has been identified as a principal component of a receptor of JA in Arabidopsisand other plants, and the JA ZIM domain (JAZ) family proteins are key regulators of JA signaling that repress transcription of JA-responsive genes through interaction with transcription factors, such as MYC2 . This transcriptional repression requires novel interactor of JAZ (NINJA) and TOPLESS corepressor proteins. Bioactive JA, the jasmonoyl-isoleucine conjugate, promotes physical interaction between COI1 and JAZ proteins that results in degradation of JAZs by the 26S proteasome, leading to initiation of JA responses. Therefore, the SCF COI1 –JAZ protein complex acts as a core site of JA perception. As a regulatory loop, JA also activates JAZ gene transcription, leading to thedown-regulation of JA action and the dynamic nature of JA response.<br /> <br /> ===Expression===<br /> The transcript levels of both OsCOI1a and OsCOI1bwere significantly reduced in these RNAi lines as detected by RNA blot and quantitative RT-PCR (qRT-PCR)<br /> analyses (Fig. 1 A and B), indicating that OsCOI1 expression was effectively knocked down by RNAi.<br /> <br /> [[File:1.png]]<br /> <br /> <br /> (A) Suppression of OsCOI1a expression in transgenic RNAi lines as shown by RNA blot analysis. A fragment of 825 to 1,244 nt ofOsCOI1 was used as the probe, and 25SrRNAwas used as the loading control.<br /> <br /> <br /> [[File:2.jpg]]<br /> <br /> <br /> (B) Suppression ofOsCOI1b expression in transgenic RNAi lines as shown by qRTPCR.<br /> <br /> <br /> 2.Elongation of OsCOI1-RNAi Plants Is Inhibited by Attenuating GA Signaling. <br /> EUI1 overexpression resulted in a series of GA-deficient phenotypes, with drastic reduction of the bioactive GAs and accumulation or stabilization of the DELLA protein SLR1 .We crossed the EUI1-overexpression plants (Eui1-OX) to the coi1-18 plant (Fig. 3D). The homozygous Eui1-OX/coi1-18 plants showed greatly reduced plant height (Fig. 3 A–C) and reduced cell size in the uppermost internode (Fig. 3 E and F), similar to Eui1-OX plants. In addition, the longer grain phenotype of the coi1-18 plants was also reverted to that of the WT (Fig. 3 G and H). We also crossed coi1-18 with the GA receptor gid1-1 mutant. Again, the gid1-1/coi1-18 double mutant exhibited a dwarf phenotype like gid1-1. This result demonstrated that the GA receptor gene GID1 is required for the function of OsCOI1 in the GA pathway. These results suggest that the OsCOI1-RNAi morphology is dependent on the GA signaling pathway.<br /> [[File:7.jpg]]<br /> <br /> <br /> Fig. 3. The GA-deficiency mutation reverses the phenotype of coi1-18plants. (A) Morphological phenotype of Eui1-OX/coi1-18. (B) The average plant height of WT, coi1-18, Eui1-OX, and Eui1-OX/coi1-18 plants. (C) The length of each internode of coi1-18 decreased in the Eui1-OX/coi1-18 plants. (D) Expression of OsCOI1 in Eui1-OX/coi1-18. (E and F) Cell lengths at the bases of the uppermost internodes in coi1-18, Eui1-OX/coi1-18, and Eui1-OX plants. (Scale bar, 40 μm.) (G and H) Grain lengths of coi1-18, Eui1-OX/coi1-18, and Eui1-OX plants. Letters on the columns in B, C, F, and H indicate significant differences determined by Tukey–Kramer multiple comparison test (P &lt; 0.05).<br /> <br /> ===Evolution===<br /> The rice genome contains two closely related COI1genes, OsCOI1a(Os01g0853400) and OsCOI1b(Os05g0449500), which share 83% and 82% sequence identity at the DNA and protein levels, respectively.<br /> <br /> ==Labs working on this gene==<br /> National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy<br /> of Sciences, Shanghai 200032, China;<br /> <br /> ==References==<br /> Yang, D.L., et al., Plant hormone jasmonate prioritizes defense over growth by interfering with gibberellin signaling cascade. Proc Natl Acad Sci U S A, 2012. 109(19): p. E1192-200.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0853400|<br /> Description = Similar to Coronatine-insensitive protein 1 (F-box/LRR-repeat protein 2) (AtFBL2) (COI-1) (AtCOI1)|<br /> Version = NM_001051366.2 GI:297597978 GeneID:4324818|<br /> Length = 3825 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0853400, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:38502654..38506478|<br /> CDS = 38502654..38502710,38503400..38503953,38505117..38505591,38505672..38506478|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:38502654..38506478<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:38502654..38506478<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcctccgtatgaaacagctagggcagagccagccagcaacaacaagcagcggccgatctccggcgagacggccgctgggagcagccgatccggccccgatccgatgggtggcgaggtgccggagccgcggcggctcaaccgggcgctcagcttcgacgactgggtccccgacgaggcgctgcacctcgtgatgggccacgtcgaggacccgcgggacagggaggcggcgtcgcgggtgtgccgccgctggcaccgcatcgacgcgctcacgcgcaagcacgtcaccgtcgccttctgctacgccgcgcgccccgcgcgcctccgggagcggttcccgcggctcgagtcgctctcgctcaagggcaagccccgcgccgccatgtacgggctcatccccgacgactggggcgcctacgccgcgccatggatcgacgagctcgccgcgccgctcgagtgcctcaaggcgctccacctccgccgcatgaccgtcaccgacgccgacatcgccgcccttgtccgcgcccgcggacacatgctgcaggagctcaagctcgacaagtgcatcggcttctccactgacgccctccgcctcgtcgcccgctcgtgcagatccctgagaactttatttctggaagagtgccatattactgataagggtggtgaatggcttcatgaacttgctgtcaacaattctgttctggtgacactgaacttctacatgactgaactcaaagtggcgccagctgatctagagcttcttgcaaagaattgcaagtcattgatttcattgaagatgagtgagtgtgatctttcagatctgattagtttttttcaaacagccaatgcgctgcaagactttgctggaggagcattctacgaggtaggagagctcaccaagtatgaaaaagttaagttcccacccagattatgcttcttggggcttacctacatgggaacaaatgagatgcctgttatcttccctttttcgatgaaactcaagaaactggacttgcaatacacttttctcacaacagaagatcattgtcagattattgcaaaatgtcccaatctactaattcttgaggtgaggaacgtgataggagatagagggctagaagttgttggtgatacatgcaagaagctacgaagactccgaattgagcggggtgatgatgatccaggtctgcaggaagagcaaggaggagtttctcagctaggcttgacagccgttgctgttggttgccgtgaattggagtacatagctgcctatgtatcggatatcaccaatggggccctggagtctattgggactttctgcaaaaatctatacgactttcggcttgtgctacttgacagagaaagacaggtaacagatctgccacttgacaatggtgtctgtgctctgttaagaaattgcacaaagcttcggaggtttgctctctaccttagaccaggagggctttcagatgatggccttagctacatcggacagtacagtggaaatatccaatacatgctactgggcaatgttggtgaatctgaccatggattgatccgtttcgcagtgggctgcaccaaccttcagaagcttgaattgagaagctgctgcttcagcgagcgagctttgtccctcgctgtactgcagatgccctccctgagatacatatgggtgcaaggatacagagcatctcaaacaggccttgacctcctgctcatggccaggcctttctggaacatcgagtttacacctccgagccctgagagttttaatcatatgacagaagatggagaaccctgtgtggatagccatgctcaggttcttgcctactattcccttgctggaaggaggtctgactgccctcagtgggtgatccccttgcatcctgcgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MPPYETARAEPASNNKQRPISGETAAGSSRSGPDPMGGEVPEPR RLNRALSFDDWVPDEALHLVMGHVEDPRDREAASRVCRRWHRIDALTRKHVTVAFCYA ARPARLRERFPRLESLSLKGKPRAAMYGLIPDDWGAYAAPWIDELAAPLECLKALHLR RMTVTDADIAALVRARGHMLQELKLDKCIGFSTDALRLVARSCRSLRTLFLEECHITD KGGEWLHELAVNNSVLVTLNFYMTELKVAPADLELLAKNCKSLISLKMSECDLSDLIS FFQTANALQDFAGGAFYEVGELTKYEKVKFPPRLCFLGLTYMGTNEMPVIFPFSMKLK KLDLQYTFLTTEDHCQIIAKCPNLLILEVRNVIGDRGLEVVGDTCKKLRRLRIERGDD DPGLQEEQGGVSQLGLTAVAVGCRELEYIAAYVSDITNGALESIGTFCKNLYDFRLVL LDRERQVTDLPLDNGVCALLRNCTKLRRFALYLRPGGLSDDGLSYIGQYSGNIQYMLL GNVGESDHGLIRFAVGCTNLQKLELRSCCFSERALSLAVLQMPSLRYIWVQGYRASQT GLDLLLMARPFWNIEFTPPSPESFNHMTEDGEPCVDSHAQVLAYYSLAGRRSDCPQWV IPLHPA&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1..57#747..1300#2464..2938#3019..3825#atgcctccgtatgaaacagctagggcagagccagccagcaacaacaagcagcggccggtacgcaataatggaactctccataagccaagcagaggagagagcggtgcggatgcaacagagtactgagagaaaacagagtattttacgcactgttgtcggctagctgtatccgtttgcgtttcagggtaaatccatccatcatcgatccattcatggtggtgggctgctggtggtggtggtggtgctatgctacaggttaatttaggcgatgcttacgtgatgctaacacccagttaacgtggctagggctacgctaagcaaggagcattgaattcgatcgatatgttgggttttttctctttactagtagcatgccatctagtgtgcatcttacgtagtggaatattatcgggcacccaatattcggctcgcacaaagctccgaggacgaggaggaggacgactaatttggcagctcagctcacctgcacggctgcactgtgctgtgcccggtgggcgaagccatttcacccgcgggcttacgtggcccggcaccaccggccgagccgtgcggggaaacaagtaaacgctcgctcgctgcgtctccgatgcccccgtccccgcctggccgctgctgctctctcgctcttctccctccaataattcgcttgccctctggtggctcaaagcccagggaaattgatggagaagaattggggtagccccatgccacctggttcgggctagatctccggcgagacggccgctgggagcagccgatccggccccgatccgatgggtggcgaggtgccggagccgcggcggctcaaccgggcgctcagcttcgacgactgggtccccgacgaggcgctgcacctcgtgatgggccacgtcgaggacccgcgggacagggaggcggcgtcgcgggtgtgccgccgctggcaccgcatcgacgcgctcacgcgcaagcacgtcaccgtcgccttctgctacgccgcgcgccccgcgcgcctccgggagcggttcccgcggctcgagtcgctctcgctcaagggcaagccccgcgccgccatgtacgggctcatccccgacgactggggcgcctacgccgcgccatggatcgacgagctcgccgcgccgctcgagtgcctcaaggcgctccacctccgccgcatgaccgtcaccgacgccgacatcgccgcccttgtccgcgcccgcggacacatgctgcaggagctcaagctcgacaagtgcatcggcttctccactgacgccctccgcctcgtcgcccgctcgtgcaggtaacccaggagttctctctttcttgtactactcatctgtaacagtgattttttttacctccaatacttgcctaagcgaaagttagcagcagcaactgagcagttggtcttttcctcatttagaagttagggttgatctggtggaggtgctaattttgttgttagttggtgttcctaaatggagtaatggatagtagtgacatgttcttagtgttctaacatgggcaagcaaagttgagccaaccttgctcactttctggttcacaaattcctcccccccccccccctcgagaaaggagctgtcacaaattagcttccacagtccactcgtgactggttgaggccataacgcaccgaatttcaacagcaaaatttgtgagcattagatgcgcacaagatttttgcctcaatagatttgtgtggttctttcgtttacctactttcattatttcatctaattgtgtacttgttgagctgggtttgagaagagtccctaatacataaaaacaacttataagaggtagcaagtctggcataagcaatctgctataatttgaggcagacccataacggttggtcatatatcttttaccaggagtaagtactattgtaatggttggattgtacttgaaaaggactaccctgttcctccttagacatatttggaaccaaaatacagttgcaagttggggattatgttgccgccttgccggtcagttatatcatgctccgatttgtgcggcagtatgtcttgtctcatgtttcttgttaactttgcttagtttgactttgagttcgcaacaatgatgtcgcaccaattatccgttaaaaaaaatttataaagaacgcaagaatattttgtgctaaaggtatatatttatttttgagacctaacttgtgaagtctgcatgcatatggagatgtgtggggtggatgaccagtaataccttgtgtggaatgcatgtatgggatgctttgtgatggttggaagtacttcacaagtcactacacatctactatgagttgctatgtttcttgacttaaaatgtgattcctgttctttcctccaatatatatttcttctaacagcaatttcttcatgctcaatgctaagattacatgctatctctgcagtaacatgtcattctaaattatgtcaacagatccctgagaactttatttctggaagagtgccatattactgataagggtggtgaatggcttcatgaacttgctgtcaacaattctgttctggtgacactgaacttctacatgactgaactcaaagtggcgccagctgatctagagcttcttgcaaagaattgcaagtcattgatttcattgaagatgagtgagtgtgatctttcagatctgattagtttttttcaaacagccaatgcgctgcaagactttgctggaggagcattctacgaggtaggagagctcaccaagtatgaaaaagttaagttcccacccagattatgcttcttggggcttacctacatgggaacaaatgagatgcctgttatcttccctttttcgatgaaactcaagaaactggacttgcaatacacttttctcacaacagaagatcattgtcagattattgcaaaatgtcccaatctactaattcttgaggtaatatttcctgtatacaagcattgattaattgtgtttgaattagtatatgccatattacaacgagatgtcctcaacaggtgaggaacgtgataggagatagagggctagaagttgttggtgatacatgcaagaagctacgaagactccgaattgagcggggtgatgatgatccaggtctgcaggaagagcaaggaggagtttctcagctaggcttgacagccgttgctgttggttgccgtgaattggagtacatagctgcctatgtatcggatatcaccaatggggccctggagtctattgggactttctgcaaaaatctatacgactttcggcttgtgctacttgacagagaaagacaggtaacagatctgccacttgacaatggtgtctgtgctctgttaagaaattgcacaaagcttcggaggtttgctctctaccttagaccaggagggctttcagatgatggccttagctacatcggacagtacagtggaaatatccaatacatgctactgggcaatgttggtgaatctgaccatggattgatccgtttcgcagtgggctgcaccaaccttcagaagcttgaattgagaagctgctgcttcagcgagcgagctttgtccctcgctgtactgcagatgccctccctgagatacatatgggtgcaaggatacagagcatctcaaacaggccttgacctcctgctcatggccaggcctttctggaacatcgagtttacacctccgagccctgagagttttaatcatatgacagaagatggagaaccctgtgtggatagccatgctcaggttcttgcctactattcccttgctggaaggaggtctgactgccctcagtgggtgatccccttgcatcctgcgtga&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051366.2 RefSeq:Os01g0853400]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:7.jpg&diff=181203 File:7.jpg 2014-06-08T12:53:18Z <p>Jieomlong: uploaded a new version of &amp;quot;File:7.jpg&amp;quot;</p> <hr /> <div></div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0853400&diff=181200 Os01g0853400 2014-06-08T12:51:05Z <p>Jieomlong: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> The F-box protein coronatine insensitive 1 (COI1),（The rice genome contains two closely related COI1genes, OsCOI1a(Os01g0853400) and OsCOI1b(Os05g0449500), which share 83% and 82% sequence identity at the DNA and protein levels, respectively.） a component of the SCF E3 ubiquitin ligase, has been identified as a principal component of a receptor of JA in Arabidopsisand other plants, and the JA ZIM domain (JAZ) family proteins are key regulators of JA signaling that repress transcription of JA-responsive genes through interaction with transcription factors, such as MYC2 . This transcriptional repression requires novel interactor of JAZ (NINJA) and TOPLESS corepressor proteins. Bioactive JA, the jasmonoyl-isoleucine conjugate, promotes physical interaction between COI1 and JAZ proteins that results in degradation of JAZs by the 26S proteasome, leading to initiation of JA responses. Therefore, the SCF COI1 –JAZ protein complex acts as a core site of JA perception. As a regulatory loop, JA also activates JAZ gene transcription, leading to thedown-regulation of JA action and the dynamic nature of JA response.<br /> <br /> ===Expression===<br /> The transcript levels of both OsCOI1a and OsCOI1bwere significantly reduced in these RNAi lines as detected by RNA blot and quantitative RT-PCR (qRT-PCR)<br /> analyses (Fig. 1 A and B), indicating that OsCOI1 expression was effectively knocked down by RNAi.<br /> <br /> [[File:1.png]]<br /> <br /> <br /> (A) Suppression of OsCOI1a expression in transgenic RNAi lines as shown by RNA blot analysis. A fragment of 825 to 1,244 nt ofOsCOI1 was used as the probe, and 25SrRNAwas used as the loading control.<br /> <br /> <br /> [[File:2.jpg]]<br /> <br /> <br /> (B) Suppression ofOsCOI1b expression in transgenic RNAi lines as shown by qRTPCR.<br /> <br /> <br /> 2.Elongation of OsCOI1-RNAi Plants Is Inhibited by Attenuating GA Signaling. <br /> EUI1 overexpression resulted in a series of GA-deficient phenotypes, with drastic reduction of the bioactive GAs and accumulation or stabilization of the DELLA protein SLR1 .We crossed the EUI1-overexpression plants (Eui1-OX) to the coi1-18 plant (Fig. 3D). The homozygous Eui1-OX/coi1-18 plants showed greatly reduced plant height (Fig. 3 A–C) and reduced cell size in the uppermost internode (Fig. 3 E and F), similar to Eui1-OX plants. In addition, the longer grain phenotype of the coi1-18 plants was also reverted to that of the WT (Fig. 3 G and H). We also crossed coi1-18 with the GA receptor gid1-1 mutant. Again, the gid1-1/coi1-18 double mutant exhibited a dwarf phenotype like gid1-1. This result demonstrated that the GA receptor gene GID1 is required for the function of OsCOI1 in the GA pathway. These results suggest that the OsCOI1-RNAi morphology is dependent on the GA signaling pathway.<br /> <br /> ===Evolution===<br /> The rice genome contains two closely related COI1genes, OsCOI1a(Os01g0853400) and OsCOI1b(Os05g0449500), which share 83% and 82% sequence identity at the DNA and protein levels, respectively.<br /> <br /> ==Labs working on this gene==<br /> National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy<br /> of Sciences, Shanghai 200032, China;<br /> <br /> ==References==<br /> Yang, D.L., et al., Plant hormone jasmonate prioritizes defense over growth by interfering with gibberellin signaling cascade. Proc Natl Acad Sci U S A, 2012. 109(19): p. E1192-200.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0853400|<br /> Description = Similar to Coronatine-insensitive protein 1 (F-box/LRR-repeat protein 2) (AtFBL2) (COI-1) (AtCOI1)|<br /> Version = NM_001051366.2 GI:297597978 GeneID:4324818|<br /> Length = 3825 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0853400, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:38502654..38506478|<br /> CDS = 38502654..38502710,38503400..38503953,38505117..38505591,38505672..38506478|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:38502654..38506478<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:38502654..38506478<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcctccgtatgaaacagctagggcagagccagccagcaacaacaagcagcggccgatctccggcgagacggccgctgggagcagccgatccggccccgatccgatgggtggcgaggtgccggagccgcggcggctcaaccgggcgctcagcttcgacgactgggtccccgacgaggcgctgcacctcgtgatgggccacgtcgaggacccgcgggacagggaggcggcgtcgcgggtgtgccgccgctggcaccgcatcgacgcgctcacgcgcaagcacgtcaccgtcgccttctgctacgccgcgcgccccgcgcgcctccgggagcggttcccgcggctcgagtcgctctcgctcaagggcaagccccgcgccgccatgtacgggctcatccccgacgactggggcgcctacgccgcgccatggatcgacgagctcgccgcgccgctcgagtgcctcaaggcgctccacctccgccgcatgaccgtcaccgacgccgacatcgccgcccttgtccgcgcccgcggacacatgctgcaggagctcaagctcgacaagtgcatcggcttctccactgacgccctccgcctcgtcgcccgctcgtgcagatccctgagaactttatttctggaagagtgccatattactgataagggtggtgaatggcttcatgaacttgctgtcaacaattctgttctggtgacactgaacttctacatgactgaactcaaagtggcgccagctgatctagagcttcttgcaaagaattgcaagtcattgatttcattgaagatgagtgagtgtgatctttcagatctgattagtttttttcaaacagccaatgcgctgcaagactttgctggaggagcattctacgaggtaggagagctcaccaagtatgaaaaagttaagttcccacccagattatgcttcttggggcttacctacatgggaacaaatgagatgcctgttatcttccctttttcgatgaaactcaagaaactggacttgcaatacacttttctcacaacagaagatcattgtcagattattgcaaaatgtcccaatctactaattcttgaggtgaggaacgtgataggagatagagggctagaagttgttggtgatacatgcaagaagctacgaagactccgaattgagcggggtgatgatgatccaggtctgcaggaagagcaaggaggagtttctcagctaggcttgacagccgttgctgttggttgccgtgaattggagtacatagctgcctatgtatcggatatcaccaatggggccctggagtctattgggactttctgcaaaaatctatacgactttcggcttgtgctacttgacagagaaagacaggtaacagatctgccacttgacaatggtgtctgtgctctgttaagaaattgcacaaagcttcggaggtttgctctctaccttagaccaggagggctttcagatgatggccttagctacatcggacagtacagtggaaatatccaatacatgctactgggcaatgttggtgaatctgaccatggattgatccgtttcgcagtgggctgcaccaaccttcagaagcttgaattgagaagctgctgcttcagcgagcgagctttgtccctcgctgtactgcagatgccctccctgagatacatatgggtgcaaggatacagagcatctcaaacaggccttgacctcctgctcatggccaggcctttctggaacatcgagtttacacctccgagccctgagagttttaatcatatgacagaagatggagaaccctgtgtggatagccatgctcaggttcttgcctactattcccttgctggaaggaggtctgactgccctcagtgggtgatccccttgcatcctgcgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MPPYETARAEPASNNKQRPISGETAAGSSRSGPDPMGGEVPEPR RLNRALSFDDWVPDEALHLVMGHVEDPRDREAASRVCRRWHRIDALTRKHVTVAFCYA ARPARLRERFPRLESLSLKGKPRAAMYGLIPDDWGAYAAPWIDELAAPLECLKALHLR RMTVTDADIAALVRARGHMLQELKLDKCIGFSTDALRLVARSCRSLRTLFLEECHITD KGGEWLHELAVNNSVLVTLNFYMTELKVAPADLELLAKNCKSLISLKMSECDLSDLIS FFQTANALQDFAGGAFYEVGELTKYEKVKFPPRLCFLGLTYMGTNEMPVIFPFSMKLK KLDLQYTFLTTEDHCQIIAKCPNLLILEVRNVIGDRGLEVVGDTCKKLRRLRIERGDD DPGLQEEQGGVSQLGLTAVAVGCRELEYIAAYVSDITNGALESIGTFCKNLYDFRLVL LDRERQVTDLPLDNGVCALLRNCTKLRRFALYLRPGGLSDDGLSYIGQYSGNIQYMLL GNVGESDHGLIRFAVGCTNLQKLELRSCCFSERALSLAVLQMPSLRYIWVQGYRASQT GLDLLLMARPFWNIEFTPPSPESFNHMTEDGEPCVDSHAQVLAYYSLAGRRSDCPQWV IPLHPA&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1..57#747..1300#2464..2938#3019..3825#atgcctccgtatgaaacagctagggcagagccagccagcaacaacaagcagcggccggtacgcaataatggaactctccataagccaagcagaggagagagcggtgcggatgcaacagagtactgagagaaaacagagtattttacgcactgttgtcggctagctgtatccgtttgcgtttcagggtaaatccatccatcatcgatccattcatggtggtgggctgctggtggtggtggtggtgctatgctacaggttaatttaggcgatgcttacgtgatgctaacacccagttaacgtggctagggctacgctaagcaaggagcattgaattcgatcgatatgttgggttttttctctttactagtagcatgccatctagtgtgcatcttacgtagtggaatattatcgggcacccaatattcggctcgcacaaagctccgaggacgaggaggaggacgactaatttggcagctcagctcacctgcacggctgcactgtgctgtgcccggtgggcgaagccatttcacccgcgggcttacgtggcccggcaccaccggccgagccgtgcggggaaacaagtaaacgctcgctcgctgcgtctccgatgcccccgtccccgcctggccgctgctgctctctcgctcttctccctccaataattcgcttgccctctggtggctcaaagcccagggaaattgatggagaagaattggggtagccccatgccacctggttcgggctagatctccggcgagacggccgctgggagcagccgatccggccccgatccgatgggtggcgaggtgccggagccgcggcggctcaaccgggcgctcagcttcgacgactgggtccccgacgaggcgctgcacctcgtgatgggccacgtcgaggacccgcgggacagggaggcggcgtcgcgggtgtgccgccgctggcaccgcatcgacgcgctcacgcgcaagcacgtcaccgtcgccttctgctacgccgcgcgccccgcgcgcctccgggagcggttcccgcggctcgagtcgctctcgctcaagggcaagccccgcgccgccatgtacgggctcatccccgacgactggggcgcctacgccgcgccatggatcgacgagctcgccgcgccgctcgagtgcctcaaggcgctccacctccgccgcatgaccgtcaccgacgccgacatcgccgcccttgtccgcgcccgcggacacatgctgcaggagctcaagctcgacaagtgcatcggcttctccactgacgccctccgcctcgtcgcccgctcgtgcaggtaacccaggagttctctctttcttgtactactcatctgtaacagtgattttttttacctccaatacttgcctaagcgaaagttagcagcagcaactgagcagttggtcttttcctcatttagaagttagggttgatctggtggaggtgctaattttgttgttagttggtgttcctaaatggagtaatggatagtagtgacatgttcttagtgttctaacatgggcaagcaaagttgagccaaccttgctcactttctggttcacaaattcctcccccccccccccctcgagaaaggagctgtcacaaattagcttccacagtccactcgtgactggttgaggccataacgcaccgaatttcaacagcaaaatttgtgagcattagatgcgcacaagatttttgcctcaatagatttgtgtggttctttcgtttacctactttcattatttcatctaattgtgtacttgttgagctgggtttgagaagagtccctaatacataaaaacaacttataagaggtagcaagtctggcataagcaatctgctataatttgaggcagacccataacggttggtcatatatcttttaccaggagtaagtactattgtaatggttggattgtacttgaaaaggactaccctgttcctccttagacatatttggaaccaaaatacagttgcaagttggggattatgttgccgccttgccggtcagttatatcatgctccgatttgtgcggcagtatgtcttgtctcatgtttcttgttaactttgcttagtttgactttgagttcgcaacaatgatgtcgcaccaattatccgttaaaaaaaatttataaagaacgcaagaatattttgtgctaaaggtatatatttatttttgagacctaacttgtgaagtctgcatgcatatggagatgtgtggggtggatgaccagtaataccttgtgtggaatgcatgtatgggatgctttgtgatggttggaagtacttcacaagtcactacacatctactatgagttgctatgtttcttgacttaaaatgtgattcctgttctttcctccaatatatatttcttctaacagcaatttcttcatgctcaatgctaagattacatgctatctctgcagtaacatgtcattctaaattatgtcaacagatccctgagaactttatttctggaagagtgccatattactgataagggtggtgaatggcttcatgaacttgctgtcaacaattctgttctggtgacactgaacttctacatgactgaactcaaagtggcgccagctgatctagagcttcttgcaaagaattgcaagtcattgatttcattgaagatgagtgagtgtgatctttcagatctgattagtttttttcaaacagccaatgcgctgcaagactttgctggaggagcattctacgaggtaggagagctcaccaagtatgaaaaagttaagttcccacccagattatgcttcttggggcttacctacatgggaacaaatgagatgcctgttatcttccctttttcgatgaaactcaagaaactggacttgcaatacacttttctcacaacagaagatcattgtcagattattgcaaaatgtcccaatctactaattcttgaggtaatatttcctgtatacaagcattgattaattgtgtttgaattagtatatgccatattacaacgagatgtcctcaacaggtgaggaacgtgataggagatagagggctagaagttgttggtgatacatgcaagaagctacgaagactccgaattgagcggggtgatgatgatccaggtctgcaggaagagcaaggaggagtttctcagctaggcttgacagccgttgctgttggttgccgtgaattggagtacatagctgcctatgtatcggatatcaccaatggggccctggagtctattgggactttctgcaaaaatctatacgactttcggcttgtgctacttgacagagaaagacaggtaacagatctgccacttgacaatggtgtctgtgctctgttaagaaattgcacaaagcttcggaggtttgctctctaccttagaccaggagggctttcagatgatggccttagctacatcggacagtacagtggaaatatccaatacatgctactgggcaatgttggtgaatctgaccatggattgatccgtttcgcagtgggctgcaccaaccttcagaagcttgaattgagaagctgctgcttcagcgagcgagctttgtccctcgctgtactgcagatgccctccctgagatacatatgggtgcaaggatacagagcatctcaaacaggccttgacctcctgctcatggccaggcctttctggaacatcgagtttacacctccgagccctgagagttttaatcatatgacagaagatggagaaccctgtgtggatagccatgctcaggttcttgcctactattcccttgctggaaggaggtctgactgccctcagtgggtgatccccttgcatcctgcgtga&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051366.2 RefSeq:Os01g0853400]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=181188 Os10g0127900 2014-06-08T12:39:52Z <p>Jieomlong: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> <br /> ===Expression===<br /> 1.OsFbx352 encodes a F-box domain protein and is suppressed by glucose<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.[[File:1.gif]]<br /> Fig. 1. OsFbx352 contains a conserved F-box domain.(A) Alignment of the conserved F-box domain in OsFbx352 withother representative F-box-containing proteins: blue indicates residues identical to the OsFbx352 sequence; other colours indicate similar amino acids. The sequence at the bottom is the consensus sequence. (B)Phylogenetic tree analysis of the conserved F-box domain in OsFbx352 with other known F-box proteins from diverse organisms. Alignment and phylogenetic<br /> analysis were performed using clustal x 2 and mega 5, respectively. Approximately 40 amino acids that constitute the<br /> F-box motif in OsFBX352 were aligned with comparable regions from reported F-box proteins in humans (SKP2, BAB87202.1), yeast (CDC4, NP_116585.1), Arabidopsis (COI1, NP_565919; SON1, NP_179323; UFO, NP_564368.1; TIR1, NP_567135.1), and rice (OsMAIF, NP_001047698.1; OsDRF, NP_001052823.1).<br /> <br /> 2.Generation of transgenic rice plants with altered expression of OsFbx352<br /> <br /> To characterize the function of OsFbx352, transgenic rice plants were generated by constructing a RNAi construct with part of the coding sequence fragment to knock down OsFbx352 expression, as well as an overexpression construct with the OsFbx352 full-length open reading frame driven by a maize ubiquitin promoter. Both constructs were introduced into rice calluses via Agrobacterium-mediated transformation. Several transgenic lines were obtained and subsequently confirmed<br /> by qRT-PCR. The expression of OsFbx352 in the overexpression seeds was at least 11-fold higher than that in WT seeds, while OsFbx352 transcripts in seeds of the knockdown lines were reduced by approximately 70% compared with WT seeds (Fig. 2). Among the transgenic lines, two overexpression lines (OE10, OE24) and two knockdown lines (Ri15, Ri18) were selected to study the physiological function of OsFbx352 during seed germination.[[File:2.jpg]]<br /> Fig. 2. Quantitative real-time PCR analysis of OsFbx352 expression in wild-type (WT), overexpression (OE10, OE11, OE13, OE14, and OE24), and RNAi (Ri6, Ri15, and Ri18) plants. Data are mean ± SE for three replicates. Values above columns are the changes in gene expression relative to the WT.<br /> <br /> 3.Germination of transgenic seeds displayed different sensitivity to ABA<br /> Given that seed germination is sensitive to ABA and expression of OsFbx352 was strongly induced by ABA, the role of OsFbx352 played in seed germination was evaluated. There were no differences in seed germination among WT and transgenic lines in the control medium, and both WT and transgenic seeds were fully<br /> germinated after 4 days of incubation in the control medium (Fig. 3A). Germination of WT and RNAi4 seeds was delayed when ABA was present in the incubation medium, while germination of seeds with overexpressing OsFbx352 was relatively insensitive to ABA (Fig. 3B). In addition, the inhibitory effect of ABA<br /> on germination of RNAi seeds was more evident than that of WT seeds (Fig. 3B). For instance, both WT and transgenic seeds were germinated after 4 days in the control medium, while the germination rate for WT and RNAi seeds was reduced to approximately 80 and 60%, respectively, after the same period incubation in the<br /> presence of ABA (Fig. 3A, 3B). Moreover, the inhibitory effect of ABA on seed germination of WT and RNAi seeds was increased with an increase in ABA concentrations (Fig. 3C). In addition toseed germination, overexpression of OsFbx352 also rendered the seedlings less sensitive to ABA, as evidenced by longer shoot length of overexpression lines than the WT in the presence of ABA while suppression of OsFbx352 led to a shorter shoot length than the WT.[[File:3.jpg]]<br /> Fig. 3. OsFbx352-overexpressing seeds exhibited decreased sensitivity to exogenous abscisic acid (ABA). (A, B) Seeds of wild-type (WT), OE10, OE24, Ri15, and Ri18 lines were germinated in control medium (A) or 4 μM ABA (B) for 4 days. (C) Seed germination rate for WT and transgenic rice seeds after incubation in without and with different concentrations of ABA for 4 days. Data are mean ± SE for three replicates with each replicate containing 30 seeds. Statistically significant difference from the wild type within a treatment: *, P &lt; 0.05; **, P &lt; 0.01 (this figure is available in colour at JXB online).<br /> <br /> ===Evolution===<br /> F-box proteins comprise one of the large protein families in plants. There are more than 692 and 779 F-box genes in the A. thaliana and rice genomes, respectively .Previous studies demonstrated that F-box proteins are involved in the regulation of several biological processes, including circadian clock, self-incompatibility, photomorphogenesis, floral meristem, phytohormonal signalling, and responses to abiotic stresses ; Dreher and Callis, 2007). For instance, the F-box protein TIR1/AFB can bind to the transcriptional repressor protein Aux/IAA, thus promoting their proteasomal degradation and activating auxin-induced transcription. The involvement of F-box proteins in response to both biotic and abiotic stresses has also been reported. For example, four F-box proteins from different species (DOR, OsDRF1, PvFBS1, and MAIF1) have been shown to act as key regulators in responses to biotic and abiotic stress; reported that an F-box protein, ZTL, is a major factor to control the period of circadian and early photomorphogenesis by regulating PRR5 for proteasome-dependent degradation. As a primary sugar, glucose modulates many important physiological processes ranging from seed germination to seedling development.<br /> <br /> It has been well documented that the inhibitory effect of sugaron seed germination is due to the increase in the active endogenous ABA level (Arenas-Huertero et . For example, Zhu et al. (2009) reported that a higher ABA content in imbibed seeds may account for the glucose-induced delay of rice seed germination. The present results showing that ABA contents were markedly increased in imbibed seeds of both WT and transgenic lines with altered expression of OsFbx352 when challenged with glucose are in line with those findings reported in the literature. In addition, this study found that the glucose-induced increase in ABA content in the<br /> OsFbx352-overexpressing seeds was lower than in WT seeds, while it was higher in the two RNAi seeds than in WT seeds. These results suggest that overexpression of OsFbx352 disrupts glucose-induced ABA metabolism, thus rendering the germination of overexpressing seeds less sensitive to glucose.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> 1. Song, S., X. Dai, and W.H. Zhang, A rice F-box gene, OsFbx352, is involved in glucose-delayed seed germination in rice. J Exp Bot, 2012. 63(15): p. 5559-68.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaa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/> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:3.jpg&diff=180597 File:3.jpg 2014-06-08T03:12:32Z <p>Jieomlong: uploaded a new version of &amp;quot;File:3.jpg&amp;quot;</p> <hr /> <div></div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:3.jpg&diff=180595 File:3.jpg 2014-06-08T03:10:29Z <p>Jieomlong: uploaded a new version of &amp;quot;File:3.jpg&amp;quot;</p> <hr /> <div></div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:3.jpg&diff=180593 File:3.jpg 2014-06-08T03:09:14Z <p>Jieomlong: uploaded a new version of &amp;quot;File:3.jpg&amp;quot;</p> <hr /> <div></div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:3.jpg&diff=180585 File:3.jpg 2014-06-08T03:04:12Z <p>Jieomlong: uploaded a new version of &amp;quot;File:3.jpg&amp;quot;</p> <hr /> <div></div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=180579 Os10g0127900 2014-06-08T03:00:44Z <p>Jieomlong: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> <br /> ===Expression===<br /> 1.OsFbx352 encodes a F-box domain protein and is suppressed by glucose<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.[[File:1.gif]]<br /> Fig. 1. OsFbx352 contains a conserved F-box domain.(A) Alignment of the conserved F-box domain in OsFbx352 withother representative F-box-containing proteins: blue indicates residues identical to the OsFbx352 sequence; other colours indicate similar amino acids. The sequence at the bottom is the consensus sequence. (B)Phylogenetic tree analysis of the conserved F-box domain in OsFbx352 with other known F-box proteins from diverse organisms. Alignment and phylogenetic<br /> analysis were performed using clustal x 2 and mega 5, respectively. Approximately 40 amino acids that constitute the<br /> F-box motif in OsFBX352 were aligned with comparable regions from reported F-box proteins in humans (SKP2, BAB87202.1), yeast (CDC4, NP_116585.1), Arabidopsis (COI1, NP_565919; SON1, NP_179323; UFO, NP_564368.1; TIR1, NP_567135.1), and rice (OsMAIF, NP_001047698.1; OsDRF, NP_001052823.1).<br /> <br /> 2.Generation of transgenic rice plants with altered expression of OsFbx352<br /> <br /> To characterize the function of OsFbx352, transgenic rice plants were generated by constructing a RNAi construct with part of the coding sequence fragment to knock down OsFbx352 expression, as well as an overexpression construct with the OsFbx352 full-length open reading frame driven by a maize ubiquitin promoter. Both constructs were introduced into rice calluses via Agrobacterium-mediated transformation. Several transgenic lines were obtained and subsequently confirmed<br /> by qRT-PCR. The expression of OsFbx352 in the overexpression seeds was at least 11-fold higher than that in WT seeds, while OsFbx352 transcripts in seeds of the knockdown lines were reduced by approximately 70% compared with WT seeds (Fig. 2). Among the transgenic lines, two overexpression lines (OE10, OE24) and two knockdown lines (Ri15, Ri18) were selected to study the physiological function of OsFbx352 during seed germination.[[File:2.jpg]]<br /> Fig. 2. Quantitative real-time PCR analysis of OsFbx352 expression in wild-type (WT), overexpression (OE10, OE11, OE13, OE14, and OE24), and RNAi (Ri6, Ri15, and Ri18) plants. Data are mean ± SE for three replicates. Values above columns are the changes in gene expression relative to the WT.<br /> <br /> 3.Germination of transgenic seeds displayed different sensitivity to ABA<br /> Given that seed germination is sensitive to ABA and expression of OsFbx352 was strongly induced by ABA, the role of OsFbx352 played in seed germination was evaluated. There were no differences in seed germination among WT and transgenic lines in the control medium, and both WT and transgenic seeds were fully<br /> germinated after 4 days of incubation in the control medium (Fig. 3A). Germination of WT and RNAi4 seeds was delayed when ABA was present in the incubation medium, while germination of seeds with overexpressing OsFbx352 was relatively insensitive to ABA (Fig. 3B). In addition, the inhibitory effect of ABA<br /> on germination of RNAi seeds was more evident than that of WT seeds (Fig. 3B). For instance, both WT and transgenic seeds were germinated after 4 days in the control medium, while the germination rate for WT and RNAi seeds was reduced to approximately 80 and 60%, respectively, after the same period incubation in the<br /> presence of ABA (Fig. 3A, 3B). Moreover, the inhibitory effect of ABA on seed germination of WT and RNAi seeds was increased with an increase in ABA concentrations (Fig. 3C). In addition toseed germination, overexpression of OsFbx352 also rendered the seedlings less sensitive to ABA, as evidenced by longer shoot length of overexpression lines than the WT in the presence of ABA while suppression of OsFbx352 led to a shorter shoot length than the WT.[[File:3.jpg]]<br /> Fig. 3. OsFbx352-overexpressing seeds exhibited decreased sensitivity to exogenous abscisic acid (ABA). (A, B) Seeds of wild-type (WT), OE10, OE24, Ri15, and Ri18 lines were germinated in control medium (A) or 4 μM ABA (B) for 4 days. (C) Seed germination rate for WT and transgenic rice seeds after incubation in without and with different concentrations of ABA for 4 days. Data are mean ± SE for three replicates with each replicate containing 30 seeds. Statistically significant difference from the wild type within a treatment: *, P &lt; 0.05; **, P &lt; 0.01 (this figure is available in colour at JXB online).<br /> <br /> ===Evolution===<br /> F-box proteins comprise one of the large protein families in plants. There are more than 692 and 779 F-box genes in the A. thaliana and rice genomes, respectively .Previous studies demonstrated that F-box proteins are involved in the regulation of several biological processes, including circadian clock, self-incompatibility, photomorphogenesis, floral meristem, phytohormonal signalling, and responses to abiotic stresses ; Dreher and Callis, 2007). For instance, the F-box protein TIR1/AFB can bind to the transcriptional repressor protein Aux/IAA, thus promoting their proteasomal degradation and activating auxin-induced transcription. The involvement of F-box proteins in response to both biotic and abiotic stresses has also been reported. For example, four F-box proteins from different species (DOR, OsDRF1, PvFBS1, and MAIF1) have been shown to act as key regulators in responses to biotic and abiotic stress; reported that an F-box protein, ZTL, is a major factor to control the period of circadian and early photomorphogenesis by regulating PRR5 for proteasome-dependent degradation. As a primary sugar, glucose modulates many important physiological processes ranging from seed germination to seedling development.<br /> <br /> It has been well documented that the inhibitory effect of sugaron seed germination is due to the increase in the active endogenous ABA level (Arenas-Huertero et . For example, Zhu et al. (2009) reported that a higher ABA content in imbibed seeds may account for the glucose-induced delay of rice seed germination. The present results showing that ABA contents were markedly increased in imbibed seeds of both WT and transgenic lines with altered expression of OsFbx352 when challenged with glucose are in line with those findings reported in the literature. In addition, this study found that the glucose-induced increase in ABA content in the<br /> OsFbx352-overexpressing seeds was lower than in WT seeds, while it was higher in the two RNAi seeds than in WT seeds. These results suggest that overexpression of OsFbx352 disrupts glucose-induced ABA metabolism, thus rendering the germination of overexpressing seeds less sensitive to glucose.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaactttcaagtccaaatttaaaactctcagttcatcttttaaaattttaattcaaccagtctgttcgttattttgctataaataatcacgcttatgctaatctaatcgcgtttagcgctaagcgaggggaggggcgcgtctgcgtgtggcatggcgccgtgcgcgcgagttactgtagcagcgcggatgcgcgagttacgagagaagcggaaaactgaaaacgcgcgagatctcgcgtattactccttgtcacggacagtttgagagcgggaggcctcctcccaatctccccccttccccttccatagttccatggtggcgctcttcagacgcgagcgcgaggagcaccgggtccgcttgccggtggccggcctgactcgttagccgccgtcccgtccggggcgaaaggaagcatacctcgctcactccatttggaggtaatggcttcacccaatctttcttgaatcaccattcctctctggatggatgttcttcaccaaacaatgctgtattgccgctcaatgtagcatcgtttcttgcgctaagatcttgaatcttgcttggagttgagagctggggattcatggtttattgttccgttactgtgcagcttcccccatccgcacgcgcaccagctgttcgacgaaatgccaccgcgcgctctgcccccagtggaggacgacgccggcagggactggctcggtgacctccccgaggaggtcctccaccacatcatgtccttcctagacgcgcgccaggccgtgcgaacctgtgtgctgtcgcggcggtggcgcaacctctggcgtaccgtgccttgcatcaacgctgacttcgatgagtttgatctcgtcttttaccaaggggacgacgaggactatgatgacgtgctggcgttcaaaaggtttgttaaccgtctgctggagctccgcgatcccaccgccatgatagacaccttctggcttaggtacaccaccagaccagaaggaaacacgaattccaatgaagatgcctacgggtggattagccacgctttgcagaagcaggcccgggttttagaggttgtcgtgttctgctgcttatttgagctggaccactcggtgtttacttcctgctacctgagaagaattgcgttctccggtattgttttatgcaagggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaaggtatgcatatttctattacatagtatgcatctttatttaggtgtcaatttgaaaaacttaaagttaagcatggtgttgtttcttcttgctacttcataattgcaattgtttaattacctgtacctgcaaaacttatgggcattgtttggataatgggatagggaaatgatttcccaactatcaaaaggtatctttaaatcctaaccatttattctccctcttccatttaatcctaacccttcattcatttcctaatcccaatcccacccttcatttcccattatccaaacacacccatgattcaagtgatcattttgtcgtgaaatcatgatgtgttgatacgatgaccactgatgttatcaaccactcaatgcaatatagttcatccactccagccattttttgtacacaaatcaatccgttgaacacttgtcctgctgtttctttgttggtagttttatctcaaaatgaaactggatcatttctcatgtgaggactagaatgttctagtctggatgcaatatcagggctgccaaccaaatgtgcatcttgtttgacatctgactgattattttagttgacatcagttcacatcttaccttactttttgaaagagggggttagcggtgagctggtgattgtaggttctagacccaaccctagtccagatttttcttggctgcagacatgtatgcattttatttctaagatatttatagaaaacaatattgcaattgcatatgcggccatttggtattgtgtaatcatgcatgaaatcgttggtttccggattaattatttattgcattttatattttagacagtatgttgaattgggatatgatgcaacgcacagacattatgcatttatgctacataaatttgaaaattaagagacattcgaaggcaatatgcaaacagaaagtgcttcaggattagttgaaaatctcacttctagtgcttcatcatgaaatatttgtactgttctttttactagtttttgttacatgacttgcatggaaagtatcctagcagtaagttaacatgcctgagactcgcattgaaaatatgaacatgtataatattgtttagtctggtcttttgttgacataatttcagtttctcctgtggtcttttaccatccgttatcctaaagttgaagctattatacatgcactccaaatagaattctttatcacatattgttaatgctctcaatttagttgccatattttctgattatattcatttgaatctttcgaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaaggtaaatgctttcgtccaaatgtgcactgatcctacttgattgctttttacacaagagttttcacatcgggatatagatcacctgtttttgttgctgtagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatgaaatggcaaaggatcacgaattagtttgccatcccattgaagaaaatcactctctttcgcctgtcggctacatctgctggttacagtagagtgttcagtgatcttgtttggtgctttctgctatgacgtgaaggtgagtagaaacaattgcatcttgccttgcatgtaaggtcagtggtattgtacttgtttgctgcgatcacatgatgatttgtagcaataatgatgctccaagaaaaatactgtatggctatggcgtctcctgatacagcctgccactgctttctcaacttggctggtctccggtgaaacgtattgtatttgtctctctcctaatataatcccatgttatagtgttgt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:3.jpg&diff=180575 File:3.jpg 2014-06-08T02:58:58Z <p>Jieomlong: uploaded a new version of &amp;quot;File:3.jpg&amp;quot;</p> <hr /> <div></div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=180568 Os10g0127900 2014-06-08T02:57:34Z <p>Jieomlong: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> <br /> ===Expression===<br /> 1.OsFbx352 encodes a F-box domain protein and is suppressed by glucose<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.[[File:1.gif]]<br /> Fig. 1. OsFbx352 contains a conserved F-box domain.(A) Alignment of the conserved F-box domain in OsFbx352 withother representative F-box-containing proteins: blue indicates residues identical to the OsFbx352 sequence; other colours indicate similar amino acids. The sequence at the bottom is the consensus sequence. (B)Phylogenetic tree analysis of the conserved F-box domain in OsFbx352 with other known F-box proteins from diverse organisms. Alignment and phylogenetic<br /> analysis were performed using clustal x 2 and mega 5, respectively. Approximately 40 amino acids that constitute the<br /> F-box motif in OsFBX352 were aligned with comparable regions from reported F-box proteins in humans (SKP2, BAB87202.1), yeast (CDC4, NP_116585.1), Arabidopsis (COI1, NP_565919; SON1, NP_179323; UFO, NP_564368.1; TIR1, NP_567135.1), and rice (OsMAIF, NP_001047698.1; OsDRF, NP_001052823.1).<br /> <br /> 2.Generation of transgenic rice plants with altered expression of OsFbx352<br /> <br /> To characterize the function of OsFbx352, transgenic rice plants were generated by constructing a RNAi construct with part of the coding sequence fragment to knock down OsFbx352 expression, as well as an overexpression construct with the OsFbx352 full-length open reading frame driven by a maize ubiquitin promoter. Both constructs were introduced into rice calluses via Agrobacterium-mediated transformation. Several transgenic lines were obtained and subsequently confirmed<br /> by qRT-PCR. The expression of OsFbx352 in the overexpression seeds was at least 11-fold higher than that in WT seeds, while OsFbx352 transcripts in seeds of the knockdown lines were reduced by approximately 70% compared with WT seeds (Fig. 2). Among the transgenic lines, two overexpression lines (OE10, OE24) and two knockdown lines (Ri15, Ri18) were selected to study the physiological function of OsFbx352 during seed germination.[[File:2.jpg]]<br /> Fig. 2. Quantitative real-time PCR analysis of OsFbx352 expression in wild-type (WT), overexpression (OE10, OE11, OE13, OE14, and OE24), and RNAi (Ri6, Ri15, and Ri18) plants. Data are mean ± SE for three replicates. Values above columns are the changes in gene expression relative to the WT.<br /> <br /> 3.Germination of transgenic seeds displayed different sensitivity to ABA<br /> Given that seed germination is sensitive to ABA and expression of OsFbx352 was strongly induced by ABA, the role of OsFbx352 played in seed germination was evaluated. There were no differences in seed germination among WT and transgenic lines in the control medium, and both WT and transgenic seeds were fully<br /> germinated after 4 days of incubation in the control medium (Fig. 3A). Germination of WT and RNAi4 seeds was delayed when ABA was present in the incubation medium, while germination of seeds with overexpressing OsFbx352 was relatively insensitive to ABA (Fig. 3B). In addition, the inhibitory effect of ABA<br /> on germination of RNAi seeds was more evident than that of WT seeds (Fig. 3B). For instance, both WT and transgenic seeds were germinated after 4 days in the control medium, while the germination rate for WT and RNAi seeds was reduced to approximately 80 and 60%, respectively, after the same period incubation in the<br /> presence of ABA (Fig. 3A, 3B). Moreover, the inhibitory effect of ABA on seed germination of WT and RNAi seeds was increased with an increase in ABA concentrations (Fig. 3C). In addition toseed germination, overexpression of OsFbx352 also rendered the seedlings less sensitive to ABA, as evidenced by longer shoot length of overexpression lines than the WT in the presence of ABA while suppression of OsFbx352 led to a shorter shoot length than the WT.<br /> <br /> ===Evolution===<br /> F-box proteins comprise one of the large protein families in plants. There are more than 692 and 779 F-box genes in the A. thaliana and rice genomes, respectively .Previous studies demonstrated that F-box proteins are involved in the regulation of several biological processes, including circadian clock, self-incompatibility, photomorphogenesis, floral meristem, phytohormonal signalling, and responses to abiotic stresses ; Dreher and Callis, 2007). For instance, the F-box protein TIR1/AFB can bind to the transcriptional repressor protein Aux/IAA, thus promoting their proteasomal degradation and activating auxin-induced transcription. The involvement of F-box proteins in response to both biotic and abiotic stresses has also been reported. For example, four F-box proteins from different species (DOR, OsDRF1, PvFBS1, and MAIF1) have been shown to act as key regulators in responses to biotic and abiotic stress; reported that an F-box protein, ZTL, is a major factor to control the period of circadian and early photomorphogenesis by regulating PRR5 for proteasome-dependent degradation. As a primary sugar, glucose modulates many important physiological processes ranging from seed germination to seedling development.<br /> <br /> It has been well documented that the inhibitory effect of sugaron seed germination is due to the increase in the active endogenous ABA level (Arenas-Huertero et . For example, Zhu et al. (2009) reported that a higher ABA content in imbibed seeds may account for the glucose-induced delay of rice seed germination. The present results showing that ABA contents were markedly increased in imbibed seeds of both WT and transgenic lines with altered expression of OsFbx352 when challenged with glucose are in line with those findings reported in the literature. In addition, this study found that the glucose-induced increase in ABA content in the<br /> OsFbx352-overexpressing seeds was lower than in WT seeds, while it was higher in the two RNAi seeds than in WT seeds. These results suggest that overexpression of OsFbx352 disrupts glucose-induced ABA metabolism, thus rendering the germination of overexpressing seeds less sensitive to glucose.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaactttcaagtccaaatttaaaactctcagttcatcttttaaaattttaattcaaccagtctgttcgttattttgctataaataatcacgcttatgctaatctaatcgcgtttagcgctaagcgaggggaggggcgcgtctgcgtgtggcatggcgccgtgcgcgcgagttactgtagcagcgcggatgcgcgagttacgagagaagcggaaaactgaaaacgcgcgagatctcgcgtattactccttgtcacggacagtttgagagcgggaggcctcctcccaatctccccccttccccttccatagttccatggtggcgctcttcagacgcgagcgcgaggagcaccgggtccgcttgccggtggccggcctgactcgttagccgccgtcccgtccggggcgaaaggaagcatacctcgctcactccatttggaggtaatggcttcacccaatctttcttgaatcaccattcctctctggatggatgttcttcaccaaacaatgctgtattgccgctcaatgtagcatcgtttcttgcgctaagatcttgaatcttgcttggagttgagagctggggattcatggtttattgttccgttactgtgcagcttcccccatccgcacgcgcaccagctgttcgacgaaatgccaccgcgcgctctgcccccagtggaggacgacgccggcagggactggctcggtgacctccccgaggaggtcctccaccacatcatgtccttcctagacgcgcgccaggccgtgcgaacctgtgtgctgtcgcggcggtggcgcaacctctggcgtaccgtgccttgcatcaacgctgacttcgatgagtttgatctcgtcttttaccaaggggacgacgaggactatgatgacgtgctggcgttcaaaaggtttgttaaccgtctgctggagctccgcgatcccaccgccatgatagacaccttctggcttaggtacaccaccagaccagaaggaaacacgaattccaatgaagatgcctacgggtggattagccacgctttgcagaagcaggcccgggttttagaggttgtcgtgttctgctgcttatttgagctggaccactcggtgtttacttcctgctacctgagaagaattgcgttctccggtattgttttatgcaagggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaaggtatgcatatttctattacatagtatgcatctttatttaggtgtcaatttgaaaaacttaaagttaagcatggtgttgtttcttcttgctacttcataattgcaattgtttaattacctgtacctgcaaaacttatgggcattgtttggataatgggatagggaaatgatttcccaactatcaaaaggtatctttaaatcctaaccatttattctccctcttccatttaatcctaacccttcattcatttcctaatcccaatcccacccttcatttcccattatccaaacacacccatgattcaagtgatcattttgtcgtgaaatcatgatgtgttgatacgatgaccactgatgttatcaaccactcaatgcaatatagttcatccactccagccattttttgtacacaaatcaatccgttgaacacttgtcctgctgtttctttgttggtagttttatctcaaaatgaaactggatcatttctcatgtgaggactagaatgttctagtctggatgcaatatcagggctgccaaccaaatgtgcatcttgtttgacatctgactgattattttagttgacatcagttcacatcttaccttactttttgaaagagggggttagcggtgagctggtgattgtaggttctagacccaaccctagtccagatttttcttggctgcagacatgtatgcattttatttctaagatatttatagaaaacaatattgcaattgcatatgcggccatttggtattgtgtaatcatgcatgaaatcgttggtttccggattaattatttattgcattttatattttagacagtatgttgaattgggatatgatgcaacgcacagacattatgcatttatgctacataaatttgaaaattaagagacattcgaaggcaatatgcaaacagaaagtgcttcaggattagttgaaaatctcacttctagtgcttcatcatgaaatatttgtactgttctttttactagtttttgttacatgacttgcatggaaagtatcctagcagtaagttaacatgcctgagactcgcattgaaaatatgaacatgtataatattgtttagtctggtcttttgttgacataatttcagtttctcctgtggtcttttaccatccgttatcctaaagttgaagctattatacatgcactccaaatagaattctttatcacatattgttaatgctctcaatttagttgccatattttctgattatattcatttgaatctttcgaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaaggtaaatgctttcgtccaaatgtgcactgatcctacttgattgctttttacacaagagttttcacatcgggatatagatcacctgtttttgttgctgtagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatgaaatggcaaaggatcacgaattagtttgccatcccattgaagaaaatcactctctttcgcctgtcggctacatctgctggttacagtagagtgttcagtgatcttgtttggtgctttctgctatgacgtgaaggtgagtagaaacaattgcatcttgccttgcatgtaaggtcagtggtattgtacttgtttgctgcgatcacatgatgatttgtagcaataatgatgctccaagaaaaatactgtatggctatggcgtctcctgatacagcctgccactgctttctcaacttggctggtctccggtgaaacgtattgtatttgtctctctcctaatataatcccatgttatagtgttgt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=180560 Os10g0127900 2014-06-08T02:54:00Z <p>Jieomlong: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> <br /> ===Expression===<br /> 1.OsFbx352 encodes a F-box domain protein and is suppressed by glucose<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.[[File:1.gif]]<br /> Fig. 1. OsFbx352 contains a conserved F-box domain.(A) Alignment of the conserved F-box domain in OsFbx352 withother representative F-box-containing proteins: blue indicates residues identical to the OsFbx352 sequence; other colours indicate similar amino acids. The sequence at the bottom is the consensus sequence. (B)Phylogenetic tree analysis of the conserved F-box domain in OsFbx352 with other known F-box proteins from diverse organisms. Alignment and phylogenetic<br /> analysis were performed using clustal x 2 and mega 5, respectively. Approximately 40 amino acids that constitute the<br /> F-box motif in OsFBX352 were aligned with comparable regions from reported F-box proteins in humans (SKP2, BAB87202.1), yeast (CDC4, NP_116585.1), Arabidopsis (COI1, NP_565919; SON1, NP_179323; UFO, NP_564368.1; TIR1, NP_567135.1), and rice (OsMAIF, NP_001047698.1; OsDRF, NP_001052823.1).<br /> <br /> 2.Generation of transgenic rice plants with altered expression of OsFbx352<br /> <br /> To characterize the function of OsFbx352, transgenic rice plants were generated by constructing a RNAi construct with part of the coding sequence fragment to knock down OsFbx352 expression, as well as an overexpression construct with the OsFbx352 full-length open reading frame driven by a maize ubiquitin promoter. Both constructs were introduced into rice calluses via Agrobacterium-mediated transformation. Several transgenic lines were obtained and subsequently confirmed<br /> by qRT-PCR. The expression of OsFbx352 in the overexpression seeds was at least 11-fold higher than that in WT seeds, while OsFbx352 transcripts in seeds of the knockdown lines were reduced by approximately 70% compared with WT seeds (Fig. 2). Among the transgenic lines, two overexpression lines (OE10, OE24) and two knockdown lines (Ri15, Ri18) were selected to study the physiological function of OsFbx352 during seed germination.[[File:2.jpg]]<br /> Fig. 2. Quantitative real-time PCR analysis of OsFbx352 expression in wild-type (WT), overexpression (OE10, OE11, OE13, OE14, and OE24), and RNAi (Ri6, Ri15, and Ri18) plants. Data are mean ± SE for three replicates. Values above columns are the changes in gene expression relative to the WT.<br /> <br /> ===Evolution===<br /> F-box proteins comprise one of the large protein families in plants. There are more than 692 and 779 F-box genes in the A. thaliana and rice genomes, respectively .Previous studies demonstrated that F-box proteins are involved in the regulation of several biological processes, including circadian clock, self-incompatibility, photomorphogenesis, floral meristem, phytohormonal signalling, and responses to abiotic stresses ; Dreher and Callis, 2007). For instance, the F-box protein TIR1/AFB can bind to the transcriptional repressor protein Aux/IAA, thus promoting their proteasomal degradation and activating auxin-induced transcription. The involvement of F-box proteins in response to both biotic and abiotic stresses has also been reported. For example, four F-box proteins from different species (DOR, OsDRF1, PvFBS1, and MAIF1) have been shown to act as key regulators in responses to biotic and abiotic stress; reported that an F-box protein, ZTL, is a major factor to control the period of circadian and early photomorphogenesis by regulating PRR5 for proteasome-dependent degradation. As a primary sugar, glucose modulates many important physiological processes ranging from seed germination to seedling development.<br /> <br /> It has been well documented that the inhibitory effect of sugaron seed germination is due to the increase in the active endogenous ABA level (Arenas-Huertero et . For example, Zhu et al. (2009) reported that a higher ABA content in imbibed seeds may account for the glucose-induced delay of rice seed germination. The present results showing that ABA contents were markedly increased in imbibed seeds of both WT and transgenic lines with altered expression of OsFbx352 when challenged with glucose are in line with those findings reported in the literature. In addition, this study found that the glucose-induced increase in ABA content in the<br /> OsFbx352-overexpressing seeds was lower than in WT seeds, while it was higher in the two RNAi seeds than in WT seeds. These results suggest that overexpression of OsFbx352 disrupts glucose-induced ABA metabolism, thus rendering the germination of overexpressing seeds less sensitive to glucose.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaactttcaagtccaaatttaaaactctcagttcatcttttaaaattttaattcaaccagtctgttcgttattttgctataaataatcacgcttatgctaatctaatcgcgtttagcgctaagcgaggggaggggcgcgtctgcgtgtggcatggcgccgtgcgcgcgagttactgtagcagcgcggatgcgcgagttacgagagaagcggaaaactgaaaacgcgcgagatctcgcgtattactccttgtcacggacagtttgagagcgggaggcctcctcccaatctccccccttccccttccatagttccatggtggcgctcttcagacgcgagcgcgaggagcaccgggtccgcttgccggtggccggcctgactcgttagccgccgtcccgtccggggcgaaaggaagcatacctcgctcactccatttggaggtaatggcttcacccaatctttcttgaatcaccattcctctctggatggatgttcttcaccaaacaatgctgtattgccgctcaatgtagcatcgtttcttgcgctaagatcttgaatcttgcttggagttgagagctggggattcatggtttattgttccgttactgtgcagcttcccccatccgcacgcgcaccagctgttcgacgaaatgccaccgcgcgctctgcccccagtggaggacgacgccggcagggactggctcggtgacctccccgaggaggtcctccaccacatcatgtccttcctagacgcgcgccaggccgtgcgaacctgtgtgctgtcgcggcggtggcgcaacctctggcgtaccgtgccttgcatcaacgctgacttcgatgagtttgatctcgtcttttaccaaggggacgacgaggactatgatgacgtgctggcgttcaaaaggtttgttaaccgtctgctggagctccgcgatcccaccgccatgatagacaccttctggcttaggtacaccaccagaccagaaggaaacacgaattccaatgaagatgcctacgggtggattagccacgctttgcagaagcaggcccgggttttagaggttgtcgtgttctgctgcttatttgagctggaccactcggtgtttacttcctgctacctgagaagaattgcgttctccggtattgttttatgcaagggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaaggtatgcatatttctattacatagtatgcatctttatttaggtgtcaatttgaaaaacttaaagttaagcatggtgttgtttcttcttgctacttcataattgcaattgtttaattacctgtacctgcaaaacttatgggcattgtttggataatgggatagggaaatgatttcccaactatcaaaaggtatctttaaatcctaaccatttattctccctcttccatttaatcctaacccttcattcatttcctaatcccaatcccacccttcatttcccattatccaaacacacccatgattcaagtgatcattttgtcgtgaaatcatgatgtgttgatacgatgaccactgatgttatcaaccactcaatgcaatatagttcatccactccagccattttttgtacacaaatcaatccgttgaacacttgtcctgctgtttctttgttggtagttttatctcaaaatgaaactggatcatttctcatgtgaggactagaatgttctagtctggatgcaatatcagggctgccaaccaaatgtgcatcttgtttgacatctgactgattattttagttgacatcagttcacatcttaccttactttttgaaagagggggttagcggtgagctggtgattgtaggttctagacccaaccctagtccagatttttcttggctgcagacatgtatgcattttatttctaagatatttatagaaaacaatattgcaattgcatatgcggccatttggtattgtgtaatcatgcatgaaatcgttggtttccggattaattatttattgcattttatattttagacagtatgttgaattgggatatgatgcaacgcacagacattatgcatttatgctacataaatttgaaaattaagagacattcgaaggcaatatgcaaacagaaagtgcttcaggattagttgaaaatctcacttctagtgcttcatcatgaaatatttgtactgttctttttactagtttttgttacatgacttgcatggaaagtatcctagcagtaagttaacatgcctgagactcgcattgaaaatatgaacatgtataatattgtttagtctggtcttttgttgacataatttcagtttctcctgtggtcttttaccatccgttatcctaaagttgaagctattatacatgcactccaaatagaattctttatcacatattgttaatgctctcaatttagttgccatattttctgattatattcatttgaatctttcgaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaaggtaaatgctttcgtccaaatgtgcactgatcctacttgattgctttttacacaagagttttcacatcgggatatagatcacctgtttttgttgctgtagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatgaaatggcaaaggatcacgaattagtttgccatcccattgaagaaaatcactctctttcgcctgtcggctacatctgctggttacagtagagtgttcagtgatcttgtttggtgctttctgctatgacgtgaaggtgagtagaaacaattgcatcttgccttgcatgtaaggtcagtggtattgtacttgtttgctgcgatcacatgatgatttgtagcaataatgatgctccaagaaaaatactgtatggctatggcgtctcctgatacagcctgccactgctttctcaacttggctggtctccggtgaaacgtattgtatttgtctctctcctaatataatcccatgttatagtgttgt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=180551 Os10g0127900 2014-06-08T02:51:37Z <p>Jieomlong: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> <br /> ===Expression===<br /> 1.OsFbx352 encodes a F-box domain protein and is suppressed by glucose<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.[[File:1.gif]]<br /> Fig. 1. OsFbx352 contains a conserved F-box domain.(A) Alignment of the conserved F-box domain in OsFbx352 withother representative F-box-containing proteins: blue indicates residues identical to the OsFbx352 sequence; other colours indicate similar amino acids. The sequence at the bottom is the consensus sequence. (B)Phylogenetic tree analysis of the conserved F-box domain in OsFbx352 with other known F-box proteins from diverse organisms. Alignment and phylogenetic<br /> analysis were performed using clustal x 2 and mega 5, respectively. Approximately 40 amino acids that constitute the<br /> F-box motif in OsFBX352 were aligned with comparable regions from reported F-box proteins in humans (SKP2, BAB87202.1), yeast (CDC4, NP_116585.1), Arabidopsis (COI1, NP_565919; SON1, NP_179323; UFO, NP_564368.1; TIR1, NP_567135.1), and rice (OsMAIF, NP_001047698.1; OsDRF, NP_001052823.1).<br /> <br /> 2.Generation of transgenic rice plants with altered expression of OsFbx352<br /> <br /> To characterize the function of OsFbx352, transgenic rice plants were generated by constructing a RNAi construct with part of the coding sequence fragment to knock down OsFbx352 expression, as well as an overexpression construct with the OsFbx352 full-length open reading frame driven by a maize ubiquitin promoter. Both constructs were introduced into rice calluses via Agrobacterium-mediated transformation. Several transgenic lines were obtained and subsequently confirmed<br /> by qRT-PCR. The expression of OsFbx352 in the overexpression seeds was at least 11-fold higher than that in WT seeds, while OsFbx352 transcripts in seeds of the knockdown lines were reduced by approximately 70% compared with WT seeds (Fig. 2). Among the transgenic lines, two overexpression lines (OE10, OE24) and two knockdown lines (Ri15, Ri18) were selected to study the physiological function of OsFbx352 during seed germination.[[File:2.jpg]]<br /> Fig. 2. Quantitative real-time PCR analysis of OsFbx352 expression in wild-type (WT), overexpression (OE10, OE11, OE13, OE14, and OE24), and RNAi (Ri6, Ri15, and Ri18) plants. Data are mean ± SE for three replicates. Values above columns are the changes in gene expression relative to the WT.<br /> <br /> ===Evolution===<br /> F-box proteins comprise one of the large protein families in plants. There are more than 692 and 779 F-box genes in the A. thaliana and rice genomes, respectively .Previous studies demonstrated that F-box proteins are involved in the regulation of several biological processes, including circadian clock, self-incompatibility, photomorphogenesis, floral meristem, phytohormonal signalling, and responses to abiotic stresses ; Dreher and Callis, 2007). For instance, the F-box protein TIR1/AFB can bind to the transcriptional repressor protein Aux/IAA, thus promoting their proteasomal degradation and activating auxin-induced transcription. The involvement of F-box proteins in response to both biotic and abiotic stresses has also been reported. For example, four F-box proteins from different species (DOR, OsDRF1, PvFBS1, and MAIF1) have been shown to act as key regulators in responses to biotic and abiotic stress; reported that an F-box protein, ZTL, is a major factor to control the period of circadian and early photomorphogenesis by regulating PRR5 for proteasome-dependent degradation. As a primary sugar, glucose<br /> modulates many important physiological processes ranging from seed germination to seedling development<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaactttcaagtccaaatttaaaactctcagttcatcttttaaaattttaattcaaccagtctgttcgttattttgctataaataatcacgcttatgctaatctaatcgcgtttagcgctaagcgaggggaggggcgcgtctgcgtgtggcatggcgccgtgcgcgcgagttactgtagcagcgcggatgcgcgagttacgagagaagcggaaaactgaaaacgcgcgagatctcgcgtattactccttgtcacggacagtttgagagcgggaggcctcctcccaatctccccccttccccttccatagttccatggtggcgctcttcagacgcgagcgcgaggagcaccgggtccgcttgccggtggccggcctgactcgttagccgccgtcccgtccggggcgaaaggaagcatacctcgctcactccatttggaggtaatggcttcacccaatctttcttgaatcaccattcctctctggatggatgttcttcaccaaacaatgctgtattgccgctcaatgtagcatcgtttcttgcgctaagatcttgaatcttgcttggagttgagagctggggattcatggtttattgttccgttactgtgcagcttcccccatccgcacgcgcaccagctgttcgacgaaatgccaccgcgcgctctgcccccagtggaggacgacgccggcagggactggctcggtgacctccccgaggaggtcctccaccacatcatgtccttcctagacgcgcgccaggccgtgcgaacctgtgtgctgtcgcggcggtggcgcaacctctggcgtaccgtgccttgcatcaacgctgacttcgatgagtttgatctcgtcttttaccaaggggacgacgaggactatgatgacgtgctggcgttcaaaaggtttgttaaccgtctgctggagctccgcgatcccaccgccatgatagacaccttctggcttaggtacaccaccagaccagaaggaaacacgaattccaatgaagatgcctacgggtggattagccacgctttgcagaagcaggcccgggttttagaggttgtcgtgttctgctgcttatttgagctggaccactcggtgtttacttcctgctacctgagaagaattgcgttctccggtattgttttatgcaagggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaaggtatgcatatttctattacatagtatgcatctttatttaggtgtcaatttgaaaaacttaaagttaagcatggtgttgtttcttcttgctacttcataattgcaattgtttaattacctgtacctgcaaaacttatgggcattgtttggataatgggatagggaaatgatttcccaactatcaaaaggtatctttaaatcctaaccatttattctccctcttccatttaatcctaacccttcattcatttcctaatcccaatcccacccttcatttcccattatccaaacacacccatgattcaagtgatcattttgtcgtgaaatcatgatgtgttgatacgatgaccactgatgttatcaaccactcaatgcaatatagttcatccactccagccattttttgtacacaaatcaatccgttgaacacttgtcctgctgtttctttgttggtagttttatctcaaaatgaaactggatcatttctcatgtgaggactagaatgttctagtctggatgcaatatcagggctgccaaccaaatgtgcatcttgtttgacatctgactgattattttagttgacatcagttcacatcttaccttactttttgaaagagggggttagcggtgagctggtgattgtaggttctagacccaaccctagtccagatttttcttggctgcagacatgtatgcattttatttctaagatatttatagaaaacaatattgcaattgcatatgcggccatttggtattgtgtaatcatgcatgaaatcgttggtttccggattaattatttattgcattttatattttagacagtatgttgaattgggatatgatgcaacgcacagacattatgcatttatgctacataaatttgaaaattaagagacattcgaaggcaatatgcaaacagaaagtgcttcaggattagttgaaaatctcacttctagtgcttcatcatgaaatatttgtactgttctttttactagtttttgttacatgacttgcatggaaagtatcctagcagtaagttaacatgcctgagactcgcattgaaaatatgaacatgtataatattgtttagtctggtcttttgttgacataatttcagtttctcctgtggtcttttaccatccgttatcctaaagttgaagctattatacatgcactccaaatagaattctttatcacatattgttaatgctctcaatttagttgccatattttctgattatattcatttgaatctttcgaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaaggtaaatgctttcgtccaaatgtgcactgatcctacttgattgctttttacacaagagttttcacatcgggatatagatcacctgtttttgttgctgtagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatgaaatggcaaaggatcacgaattagtttgccatcccattgaagaaaatcactctctttcgcctgtcggctacatctgctggttacagtagagtgttcagtgatcttgtttggtgctttctgctatgacgtgaaggtgagtagaaacaattgcatcttgccttgcatgtaaggtcagtggtattgtacttgtttgctgcgatcacatgatgatttgtagcaataatgatgctccaagaaaaatactgtatggctatggcgtctcctgatacagcctgccactgctttctcaacttggctggtctccggtgaaacgtattgtatttgtctctctcctaatataatcccatgttatagtgttgt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=180550 Os10g0127900 2014-06-08T02:50:37Z <p>Jieomlong: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> <br /> ===Expression===<br /> 1.OsFbx352 encodes a F-box domain protein and is suppressed by glucose<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.[[File:1.gif]]<br /> Fig. 1. OsFbx352 contains a conserved F-box domain.(A) Alignment of the conserved F-box domain in OsFbx352 withother representative F-box-containing proteins: blue indicates residues identical to the OsFbx352 sequence; other colours indicate similar amino acids. The sequence at the bottom is the consensus sequence. (B)Phylogenetic tree analysis of the conserved F-box domain in OsFbx352 with other known F-box proteins from diverse organisms. Alignment and phylogenetic<br /> analysis were performed using clustal x 2 and mega 5, respectively. Approximately 40 amino acids that constitute the<br /> F-box motif in OsFBX352 were aligned with comparable regions from reported F-box proteins in humans (SKP2, BAB87202.1), yeast (CDC4, NP_116585.1), Arabidopsis (COI1, NP_565919; SON1, NP_179323; UFO, NP_564368.1; TIR1, NP_567135.1), and rice (OsMAIF, NP_001047698.1; OsDRF, NP_001052823.1).<br /> <br /> 2.Generation of transgenic rice plants with altered expression of OsFbx352<br /> <br /> To characterize the function of OsFbx352, transgenic rice plants were generated by constructing a RNAi construct with part of the coding sequence fragment to knock down OsFbx352 expression, as well as an overexpression construct with the OsFbx352 full-length open reading frame driven by a maize ubiquitin promoter. Both constructs were introduced into rice calluses via Agrobacterium-mediated transformation. Several transgenic lines were obtained and subsequently confirmed<br /> by qRT-PCR. The expression of OsFbx352 in the overexpression seeds was at least 11-fold higher than that in WT seeds, while OsFbx352 transcripts in seeds of the knockdown lines were reduced by approximately 70% compared with WT seeds (Fig. 2). Among the transgenic lines, two overexpression lines (OE10, OE24) and two knockdown lines (Ri15, Ri18) were selected to study the physiological function of OsFbx352 during seed germination.[[File:2.jpg]]<br /> Fig. 2. Quantitative real-time PCR analysis of OsFbx352 expression in wild-type (WT), overexpression (OE10, OE11, OE13, OE14, and OE24), and RNAi (Ri6, Ri15, and Ri18) plants. Data are mean ± SE for three replicates. Values above columns are the changes in gene expression relative to the WT.<br /> <br /> ===Evolution===<br /> F-box proteins comprise one of the large protein families in plants. There are more than 692 and 779 F-box genes in the A. thaliana and rice genomes, respectively .Previous studies demonstrated that F-box proteins are involved in the regulation of several biological processes, including circadian clock, self-incompatibility, photomorphogenesis, floral meristem, phytohormonal signalling, and responses to abiotic stresses ; Dreher and Callis, 2007). For instance, the F-box protein TIR1/AFB can bind to the transcriptional repressor protein Aux/IAA, thus promoting their proteasomal degradation and activating auxin-induced transcription. The involvement of F-box proteins in response to both biotic and abiotic stresses has also been reported. For example, four F-box proteins from different species (DOR, OsDRF1, PvFBS1, and MAIF1) have been shown to act as key regulators in responses to biotic and abiotic stress; reported that an F-box protein, ZTL, is a major factor to control the period of circadian and early photomorphogenesis by regulating PRR5 for proteasome-dependent degradation. As a primary sugar, glucose<br /> modulates many important physiological processes ranging from seed germination to seedling development (Smeekens,<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaactttcaagtccaaatttaaaactctcagttcatcttttaaaattttaattcaaccagtctgttcgttattttgctataaataatcacgcttatgctaatctaatcgcgtttagcgctaagcgaggggaggggcgcgtctgcgtgtggcatggcgccgtgcgcgcgagttactgtagcagcgcggatgcgcgagttacgagagaagcggaaaactgaaaacgcgcgagatctcgcgtattactccttgtcacggacagtttgagagcgggaggcctcctcccaatctccccccttccccttccatagttccatggtggcgctcttcagacgcgagcgcgaggagcaccgggtccgcttgccggtggccggcctgactcgttagccgccgtcccgtccggggcgaaaggaagcatacctcgctcactccatttggaggtaatggcttcacccaatctttcttgaatcaccattcctctctggatggatgttcttcaccaaacaatgctgtattgccgctcaatgtagcatcgtttcttgcgctaagatcttgaatcttgcttggagttgagagctggggattcatggtttattgttccgttactgtgcagcttcccccatccgcacgcgcaccagctgttcgacgaaatgccaccgcgcgctctgcccccagtggaggacgacgccggcagggactggctcggtgacctccccgaggaggtcctccaccacatcatgtccttcctagacgcgcgccaggccgtgcgaacctgtgtgctgtcgcggcggtggcgcaacctctggcgtaccgtgccttgcatcaacgctgacttcgatgagtttgatctcgtcttttaccaaggggacgacgaggactatgatgacgtgctggcgttcaaaaggtttgttaaccgtctgctggagctccgcgatcccaccgccatgatagacaccttctggcttaggtacaccaccagaccagaaggaaacacgaattccaatgaagatgcctacgggtggattagccacgctttgcagaagcaggcccgggttttagaggttgtcgtgttctgctgcttatttgagctggaccactcggtgtttacttcctgctacctgagaagaattgcgttctccggtattgttttatgcaagggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaaggtatgcatatttctattacatagtatgcatctttatttaggtgtcaatttgaaaaacttaaagttaagcatggtgttgtttcttcttgctacttcataattgcaattgtttaattacctgtacctgcaaaacttatgggcattgtttggataatgggatagggaaatgatttcccaactatcaaaaggtatctttaaatcctaaccatttattctccctcttccatttaatcctaacccttcattcatttcctaatcccaatcccacccttcatttcccattatccaaacacacccatgattcaagtgatcattttgtcgtgaaatcatgatgtgttgatacgatgaccactgatgttatcaaccactcaatgcaatatagttcatccactccagccattttttgtacacaaatcaatccgttgaacacttgtcctgctgtttctttgttggtagttttatctcaaaatgaaactggatcatttctcatgtgaggactagaatgttctagtctggatgcaatatcagggctgccaaccaaatgtgcatcttgtttgacatctgactgattattttagttgacatcagttcacatcttaccttactttttgaaagagggggttagcggtgagctggtgattgtaggttctagacccaaccctagtccagatttttcttggctgcagacatgtatgcattttatttctaagatatttatagaaaacaatattgcaattgcatatgcggccatttggtattgtgtaatcatgcatgaaatcgttggtttccggattaattatttattgcattttatattttagacagtatgttgaattgggatatgatgcaacgcacagacattatgcatttatgctacataaatttgaaaattaagagacattcgaaggcaatatgcaaacagaaagtgcttcaggattagttgaaaatctcacttctagtgcttcatcatgaaatatttgtactgttctttttactagtttttgttacatgacttgcatggaaagtatcctagcagtaagttaacatgcctgagactcgcattgaaaatatgaacatgtataatattgtttagtctggtcttttgttgacataatttcagtttctcctgtggtcttttaccatccgttatcctaaagttgaagctattatacatgcactccaaatagaattctttatcacatattgttaatgctctcaatttagttgccatattttctgattatattcatttgaatctttcgaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaaggtaaatgctttcgtccaaatgtgcactgatcctacttgattgctttttacacaagagttttcacatcgggatatagatcacctgtttttgttgctgtagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatgaaatggcaaaggatcacgaattagtttgccatcccattgaagaaaatcactctctttcgcctgtcggctacatctgctggttacagtagagtgttcagtgatcttgtttggtgctttctgctatgacgtgaaggtgagtagaaacaattgcatcttgccttgcatgtaaggtcagtggtattgtacttgtttgctgcgatcacatgatgatttgtagcaataatgatgctccaagaaaaatactgtatggctatggcgtctcctgatacagcctgccactgctttctcaacttggctggtctccggtgaaacgtattgtatttgtctctctcctaatataatcccatgttatagtgttgt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=180543 Os10g0127900 2014-06-08T02:47:48Z <p>Jieomlong: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> <br /> ===Expression===<br /> 1.OsFbx352 encodes a F-box domain protein and is suppressed by glucose<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.[[File:1.gif]]<br /> Fig. 1. OsFbx352 contains a conserved F-box domain.(A) Alignment of the conserved F-box domain in OsFbx352 withother representative F-box-containing proteins: blue indicates residues identical to the OsFbx352 sequence; other colours indicate similar amino acids. The sequence at the bottom is the consensus sequence. (B)Phylogenetic tree analysis of the conserved F-box domain in OsFbx352 with other known F-box proteins from diverse organisms. Alignment and phylogenetic<br /> analysis were performed using clustal x 2 and mega 5, respectively. Approximately 40 amino acids that constitute the<br /> F-box motif in OsFBX352 were aligned with comparable regions from reported F-box proteins in humans (SKP2, BAB87202.1), yeast (CDC4, NP_116585.1), Arabidopsis (COI1, NP_565919; SON1, NP_179323; UFO, NP_564368.1; TIR1, NP_567135.1), and rice (OsMAIF, NP_001047698.1; OsDRF, NP_001052823.1).<br /> <br /> 2.Generation of transgenic rice plants with altered expression of OsFbx352<br /> <br /> To characterize the function of OsFbx352, transgenic rice plants were generated by constructing a RNAi construct with part of the coding sequence fragment to knock down OsFbx352 expression, as well as an overexpression construct with the OsFbx352 full-length open reading frame driven by a maize ubiquitin promoter. Both constructs were introduced into rice calluses via Agrobacterium-mediated transformation. Several transgenic lines were obtained and subsequently confirmed<br /> by qRT-PCR. The expression of OsFbx352 in the overexpression seeds was at least 11-fold higher than that in WT seeds, while OsFbx352 transcripts in seeds of the knockdown lines were reduced by approximately 70% compared with WT seeds (Fig. 2). Among the transgenic lines, two overexpression lines (OE10, OE24) and two knockdown lines (Ri15, Ri18) were selected to study the physiological function of OsFbx352 during seed germination.[[File:2.jpg]]<br /> Fig. 2. Quantitative real-time PCR analysis of OsFbx352 expression in wild-type (WT), overexpression (OE10, OE11, OE13, OE14, and OE24), and RNAi (Ri6, Ri15, and Ri18) plants. Data are mean ± SE for three replicates. Values above columns are the changes in gene expression relative to the WT.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaactttcaagtccaaatttaaaactctcagttcatcttttaaaattttaattcaaccagtctgttcgttattttgctataaataatcacgcttatgctaatctaatcgcgtttagcgctaagcgaggggaggggcgcgtctgcgtgtggcatggcgccgtgcgcgcgagttactgtagcagcgcggatgcgcgagttacgagagaagcggaaaactgaaaacgcgcgagatctcgcgtattactccttgtcacggacagtttgagagcgggaggcctcctcccaatctccccccttccccttccatagttccatggtggcgctcttcagacgcgagcgcgaggagcaccgggtccgcttgccggtggccggcctgactcgttagccgccgtcccgtccggggcgaaaggaagcatacctcgctcactccatttggaggtaatggcttcacccaatctttcttgaatcaccattcctctctggatggatgttcttcaccaaacaatgctgtattgccgctcaatgtagcatcgtttcttgcgctaagatcttgaatcttgcttggagttgagagctggggattcatggtttattgttccgttactgtgcagcttcccccatccgcacgcgcaccagctgttcgacgaaatgccaccgcgcgctctgcccccagtggaggacgacgccggcagggactggctcggtgacctccccgaggaggtcctccaccacatcatgtccttcctagacgcgcgccaggccgtgcgaacctgtgtgctgtcgcggcggtggcgcaacctctggcgtaccgtgccttgcatcaacgctgacttcgatgagtttgatctcgtcttttaccaaggggacgacgaggactatgatgacgtgctggcgttcaaaaggtttgttaaccgtctgctggagctccgcgatcccaccgccatgatagacaccttctggcttaggtacaccaccagaccagaaggaaacacgaattccaatgaagatgcctacgggtggattagccacgctttgcagaagcaggcccgggttttagaggttgtcgtgttctgctgcttatttgagctggaccactcggtgtttacttcctgctacctgagaagaattgcgttctccggtattgttttatgcaagggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaaggtatgcatatttctattacatagtatgcatctttatttaggtgtcaatttgaaaaacttaaagttaagcatggtgttgtttcttcttgctacttcataattgcaattgtttaattacctgtacctgcaaaacttatgggcattgtttggataatgggatagggaaatgatttcccaactatcaaaaggtatctttaaatcctaaccatttattctccctcttccatttaatcctaacccttcattcatttcctaatcccaatcccacccttcatttcccattatccaaacacacccatgattcaagtgatcattttgtcgtgaaatcatgatgtgttgatacgatgaccactgatgttatcaaccactcaatgcaatatagttcatccactccagccattttttgtacacaaatcaatccgttgaacacttgtcctgctgtttctttgttggtagttttatctcaaaatgaaactggatcatttctcatgtgaggactagaatgttctagtctggatgcaatatcagggctgccaaccaaatgtgcatcttgtttgacatctgactgattattttagttgacatcagttcacatcttaccttactttttgaaagagggggttagcggtgagctggtgattgtaggttctagacccaaccctagtccagatttttcttggctgcagacatgtatgcattttatttctaagatatttatagaaaacaatattgcaattgcatatgcggccatttggtattgtgtaatcatgcatgaaatcgttggtttccggattaattatttattgcattttatattttagacagtatgttgaattgggatatgatgcaacgcacagacattatgcatttatgctacataaatttgaaaattaagagacattcgaaggcaatatgcaaacagaaagtgcttcaggattagttgaaaatctcacttctagtgcttcatcatgaaatatttgtactgttctttttactagtttttgttacatgacttgcatggaaagtatcctagcagtaagttaacatgcctgagactcgcattgaaaatatgaacatgtataatattgtttagtctggtcttttgttgacataatttcagtttctcctgtggtcttttaccatccgttatcctaaagttgaagctattatacatgcactccaaatagaattctttatcacatattgttaatgctctcaatttagttgccatattttctgattatattcatttgaatctttcgaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaaggtaaatgctttcgtccaaatgtgcactgatcctacttgattgctttttacacaagagttttcacatcgggatatagatcacctgtttttgttgctgtagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatgaaatggcaaaggatcacgaattagtttgccatcccattgaagaaaatcactctctttcgcctgtcggctacatctgctggttacagtagagtgttcagtgatcttgtttggtgctttctgctatgacgtgaaggtgagtagaaacaattgcatcttgccttgcatgtaaggtcagtggtattgtacttgtttgctgcgatcacatgatgatttgtagcaataatgatgctccaagaaaaatactgtatggctatggcgtctcctgatacagcctgccactgctttctcaacttggctggtctccggtgaaacgtattgtatttgtctctctcctaatataatcccatgttatagtgttgt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:2.jpg&diff=180540 File:2.jpg 2014-06-08T02:46:32Z <p>Jieomlong: uploaded a new version of &amp;quot;File:2.jpg&amp;quot;</p> <hr /> <div>Gross morphology of wildtype (left) and gid1-1 (right) plants. Scale bar, 10 cm. Inset: higher magnification of gid1-1. Scale bar, 1 cm</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=180538 Os10g0127900 2014-06-08T02:46:00Z <p>Jieomlong: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> <br /> ===Expression===<br /> 1.OsFbx352 encodes a F-box domain protein and is suppressed by glucose<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.[[File:1.gif]]<br /> Fig. 1. OsFbx352 contains a conserved F-box domain.(A) Alignment of the conserved F-box domain in OsFbx352 withother representative F-box-containing proteins: blue indicates residues identical to the OsFbx352 sequence; other colours indicate similar amino acids. The sequence at the bottom is the consensus sequence. (B)Phylogenetic tree analysis of the conserved F-box domain in OsFbx352 with other known F-box proteins from diverse organisms. Alignment and phylogenetic<br /> analysis were performed using clustal x 2 and mega 5, respectively. Approximately 40 amino acids that constitute the<br /> F-box motif in OsFBX352 were aligned with comparable regions from reported F-box proteins in humans (SKP2, BAB87202.1), yeast (CDC4, NP_116585.1), Arabidopsis (COI1, NP_565919; SON1, NP_179323; UFO, NP_564368.1; TIR1, NP_567135.1), and rice (OsMAIF, NP_001047698.1; OsDRF, NP_001052823.1).<br /> <br /> 2.Generation of transgenic rice plants with altered expression of OsFbx352<br /> <br /> To characterize the function of OsFbx352, transgenic rice plants were generated by constructing a RNAi construct with part of the coding sequence fragment to knock down OsFbx352 expression, as well as an overexpression construct with the OsFbx352 full-length open reading frame driven by a maize ubiquitin promoter. Both constructs were introduced into rice calluses via Agrobacterium-mediated transformation. Several transgenic lines were obtained and subsequently confirmed<br /> by qRT-PCR. The expression of OsFbx352 in the overexpression seeds was at least 11-fold higher than that in WT seeds, while OsFbx352 transcripts in seeds of the knockdown lines were reduced by approximately 70% compared with WT seeds (Fig. 2). Among the transgenic lines, two overexpression lines (OE10, OE24) and two knockdown lines (Ri15, Ri18) were selected to study the physiological function of OsFbx352 during seed germination.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaactttcaagtccaaatttaaaactctcagttcatcttttaaaattttaattcaaccagtctgttcgttattttgctataaataatcacgcttatgctaatctaatcgcgtttagcgctaagcgaggggaggggcgcgtctgcgtgtggcatggcgccgtgcgcgcgagttactgtagcagcgcggatgcgcgagttacgagagaagcggaaaactgaaaacgcgcgagatctcgcgtattactccttgtcacggacagtttgagagcgggaggcctcctcccaatctccccccttccccttccatagttccatggtggcgctcttcagacgcgagcgcgaggagcaccgggtccgcttgccggtggccggcctgactcgttagccgccgtcccgtccggggcgaaaggaagcatacctcgctcactccatttggaggtaatggcttcacccaatctttcttgaatcaccattcctctctggatggatgttcttcaccaaacaatgctgtattgccgctcaatgtagcatcgtttcttgcgctaagatcttgaatcttgcttggagttgagagctggggattcatggtttattgttccgttactgtgcagcttcccccatccgcacgcgcaccagctgttcgacgaaatgccaccgcgcgctctgcccccagtggaggacgacgccggcagggactggctcggtgacctccccgaggaggtcctccaccacatcatgtccttcctagacgcgcgccaggccgtgcgaacctgtgtgctgtcgcggcggtggcgcaacctctggcgtaccgtgccttgcatcaacgctgacttcgatgagtttgatctcgtcttttaccaaggggacgacgaggactatgatgacgtgctggcgttcaaaaggtttgttaaccgtctgctggagctccgcgatcccaccgccatgatagacaccttctggcttaggtacaccaccagaccagaaggaaacacgaattccaatgaagatgcctacgggtggattagccacgctttgcagaagcaggcccgggttttagaggttgtcgtgttctgctgcttatttgagctggaccactcggtgtttacttcctgctacctgagaagaattgcgttctccggtattgttttatgcaagggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaaggtatgcatatttctattacatagtatgcatctttatttaggtgtcaatttgaaaaacttaaagttaagcatggtgttgtttcttcttgctacttcataattgcaattgtttaattacctgtacctgcaaaacttatgggcattgtttggataatgggatagggaaatgatttcccaactatcaaaaggtatctttaaatcctaaccatttattctccctcttccatttaatcctaacccttcattcatttcctaatcccaatcccacccttcatttcccattatccaaacacacccatgattcaagtgatcattttgtcgtgaaatcatgatgtgttgatacgatgaccactgatgttatcaaccactcaatgcaatatagttcatccactccagccattttttgtacacaaatcaatccgttgaacacttgtcctgctgtttctttgttggtagttttatctcaaaatgaaactggatcatttctcatgtgaggactagaatgttctagtctggatgcaatatcagggctgccaaccaaatgtgcatcttgtttgacatctgactgattattttagttgacatcagttcacatcttaccttactttttgaaagagggggttagcggtgagctggtgattgtaggttctagacccaaccctagtccagatttttcttggctgcagacatgtatgcattttatttctaagatatttatagaaaacaatattgcaattgcatatgcggccatttggtattgtgtaatcatgcatgaaatcgttggtttccggattaattatttattgcattttatattttagacagtatgttgaattgggatatgatgcaacgcacagacattatgcatttatgctacataaatttgaaaattaagagacattcgaaggcaatatgcaaacagaaagtgcttcaggattagttgaaaatctcacttctagtgcttcatcatgaaatatttgtactgttctttttactagtttttgttacatgacttgcatggaaagtatcctagcagtaagttaacatgcctgagactcgcattgaaaatatgaacatgtataatattgtttagtctggtcttttgttgacataatttcagtttctcctgtggtcttttaccatccgttatcctaaagttgaagctattatacatgcactccaaatagaattctttatcacatattgttaatgctctcaatttagttgccatattttctgattatattcatttgaatctttcgaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaaggtaaatgctttcgtccaaatgtgcactgatcctacttgattgctttttacacaagagttttcacatcgggatatagatcacctgtttttgttgctgtagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatgaaatggcaaaggatcacgaattagtttgccatcccattgaagaaaatcactctctttcgcctgtcggctacatctgctggttacagtagagtgttcagtgatcttgtttggtgctttctgctatgacgtgaaggtgagtagaaacaattgcatcttgccttgcatgtaaggtcagtggtattgtacttgtttgctgcgatcacatgatgatttgtagcaataatgatgctccaagaaaaatactgtatggctatggcgtctcctgatacagcctgccactgctttctcaacttggctggtctccggtgaaacgtattgtatttgtctctctcctaatataatcccatgttatagtgttgt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=180531 Os10g0127900 2014-06-08T02:42:45Z <p>Jieomlong: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> <br /> ===Expression===<br /> 1.OsFbx352 encodes a F-box domain protein and is suppressed by glucose<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.[[File:1.gif]]<br /> Fig. 1. OsFbx352 contains a conserved F-box domain.(A) Alignment of the conserved F-box domain in OsFbx352 withother representative F-box-containing proteins: blue indicates residues identical to the OsFbx352 sequence; other colours indicate similar amino acids. The sequence at the bottom is the consensus sequence. (B)Phylogenetic tree analysis of the conserved F-box domain in OsFbx352 with other known F-box proteins from diverse organisms. Alignment and phylogenetic<br /> analysis were performed using clustal x 2 and mega 5, respectively. Approximately 40 amino acids that constitute the<br /> F-box motif in OsFBX352 were aligned with comparable regions from reported F-box proteins in humans (SKP2, BAB87202.1), yeast (CDC4, NP_116585.1), Arabidopsis (COI1, NP_565919; SON1, NP_179323; UFO, NP_564368.1; TIR1, NP_567135.1), and rice (OsMAIF, NP_001047698.1; OsDRF, NP_001052823.1).<br /> 2.Generation of transgenic rice plants with altered expression of OsFbx352<br /> <br /> To characterize the function of OsFbx352, transgenic rice plants were generated by constructing a RNAi construct with part of the coding sequence fragment to knock down OsFbx352 expression, as well as an overexpression construct with the OsFbx352 full-length open reading frame driven by a maize ubiquitin promoter. Both constructs were introduced into rice calluses via Agrobacterium-mediated transformation. Several transgenic lines were obtained and subsequently confirmed<br /> by qRT-PCR. The expression of OsFbx352 in the overexpression seeds was at least 11-fold higher than that in WT seeds, while OsFbx352 transcripts in seeds of the knockdown lines were reduced by approximately 70% compared with WT seeds (Fig. 3). Among the transgenic lines, two overexpression lines (OE10, OE24) and two knockdown lines (Ri15, Ri18) were selected to study the physiological function of OsFbx352 during seed germination.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaactttcaagtccaaatttaaaactctcagttcatcttttaaaattttaattcaaccagtctgttcgttattttgctataaataatcacgcttatgctaatctaatcgcgtttagcgctaagcgaggggaggggcgcgtctgcgtgtggcatggcgccgtgcgcgcgagttactgtagcagcgcggatgcgcgagttacgagagaagcggaaaactgaaaacgcgcgagatctcgcgtattactccttgtcacggacagtttgagagcgggaggcctcctcccaatctccccccttccccttccatagttccatggtggcgctcttcagacgcgagcgcgaggagcaccgggtccgcttgccggtggccggcctgactcgttagccgccgtcccgtccggggcgaaaggaagcatacctcgctcactccatttggaggtaatggcttcacccaatctttcttgaatcaccattcctctctggatggatgttcttcaccaaacaatgctgtattgccgctcaatgtagcatcgtttcttgcgctaagatcttgaatcttgcttggagttgagagctggggattcatggtttattgttccgttactgtgcagcttcccccatccgcacgcgcaccagctgttcgacgaaatgccaccgcgcgctctgcccccagtggaggacgacgccggcagggactggctcggtgacctccccgaggaggtcctccaccacatcatgtccttcctagacgcgcgccaggccgtgcgaacctgtgtgctgtcgcggcggtggcgcaacctctggcgtaccgtgccttgcatcaacgctgacttcgatgagtttgatctcgtcttttaccaaggggacgacgaggactatgatgacgtgctggcgttcaaaaggtttgttaaccgtctgctggagctccgcgatcccaccgccatgatagacaccttctggcttaggtacaccaccagaccagaaggaaacacgaattccaatgaagatgcctacgggtggattagccacgctttgcagaagcaggcccgggttttagaggttgtcgtgttctgctgcttatttgagctggaccactcggtgtttacttcctgctacctgagaagaattgcgttctccggtattgttttatgcaagggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaaggtatgcatatttctattacatagtatgcatctttatttaggtgtcaatttgaaaaacttaaagttaagcatggtgttgtttcttcttgctacttcataattgcaattgtttaattacctgtacctgcaaaacttatgggcattgtttggataatgggatagggaaatgatttcccaactatcaaaaggtatctttaaatcctaaccatttattctccctcttccatttaatcctaacccttcattcatttcctaatcccaatcccacccttcatttcccattatccaaacacacccatgattcaagtgatcattttgtcgtgaaatcatgatgtgttgatacgatgaccactgatgttatcaaccactcaatgcaatatagttcatccactccagccattttttgtacacaaatcaatccgttgaacacttgtcctgctgtttctttgttggtagttttatctcaaaatgaaactggatcatttctcatgtgaggactagaatgttctagtctggatgcaatatcagggctgccaaccaaatgtgcatcttgtttgacatctgactgattattttagttgacatcagttcacatcttaccttactttttgaaagagggggttagcggtgagctggtgattgtaggttctagacccaaccctagtccagatttttcttggctgcagacatgtatgcattttatttctaagatatttatagaaaacaatattgcaattgcatatgcggccatttggtattgtgtaatcatgcatgaaatcgttggtttccggattaattatttattgcattttatattttagacagtatgttgaattgggatatgatgcaacgcacagacattatgcatttatgctacataaatttgaaaattaagagacattcgaaggcaatatgcaaacagaaagtgcttcaggattagttgaaaatctcacttctagtgcttcatcatgaaatatttgtactgttctttttactagtttttgttacatgacttgcatggaaagtatcctagcagtaagttaacatgcctgagactcgcattgaaaatatgaacatgtataatattgtttagtctggtcttttgttgacataatttcagtttctcctgtggtcttttaccatccgttatcctaaagttgaagctattatacatgcactccaaatagaattctttatcacatattgttaatgctctcaatttagttgccatattttctgattatattcatttgaatctttcgaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaaggtaaatgctttcgtccaaatgtgcactgatcctacttgattgctttttacacaagagttttcacatcgggatatagatcacctgtttttgttgctgtagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatgaaatggcaaaggatcacgaattagtttgccatcccattgaagaaaatcactctctttcgcctgtcggctacatctgctggttacagtagagtgttcagtgatcttgtttggtgctttctgctatgacgtgaaggtgagtagaaacaattgcatcttgccttgcatgtaaggtcagtggtattgtacttgtttgctgcgatcacatgatgatttgtagcaataatgatgctccaagaaaaatactgtatggctatggcgtctcctgatacagcctgccactgctttctcaacttggctggtctccggtgaaacgtattgtatttgtctctctcctaatataatcccatgttatagtgttgt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=180525 Os10g0127900 2014-06-08T02:40:54Z <p>Jieomlong: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> <br /> ===Expression===<br /> 1.OsFbx352 encodes a F-box domain protein and is suppressed by glucose<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.[[File:1.gif]]<br /> Fig. 1. OsFbx352 contains a conserved F-box domain.(A) Alignment of the conserved F-box domain in OsFbx352 withother representative F-box-containing proteins: blue indicates residues identical to the OsFbx352 sequence; other colours indicate similar amino acids. The sequence at the bottom is the consensus sequence. (B)Phylogenetic tree analysis of the conserved F-box domain in OsFbx352 with other known F-box proteins from diverse organisms. Alignment and phylogenetic<br /> analysis were performed using clustal x 2 and mega 5, respectively. Approximately 40 amino acids that constitute the<br /> F-box motif in OsFBX352 were aligned with comparable regions from reported F-box proteins in humans (SKP2, BAB87202.1), yeast (CDC4, NP_116585.1), Arabidopsis (COI1, NP_565919; SON1, NP_179323; UFO, NP_564368.1; TIR1, NP_567135.1), and rice (OsMAIF, NP_001047698.1; OsDRF, NP_001052823.1).<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaactttcaagtccaaatttaaaactctcagttcatcttttaaaattttaattcaaccagtctgttcgttattttgctataaataatcacgcttatgctaatctaatcgcgtttagcgctaagcgaggggaggggcgcgtctgcgtgtggcatggcgccgtgcgcgcgagttactgtagcagcgcggatgcgcgagttacgagagaagcggaaaactgaaaacgcgcgagatctcgcgtattactccttgtcacggacagtttgagagcgggaggcctcctcccaatctccccccttccccttccatagttccatggtggcgctcttcagacgcgagcgcgaggagcaccgggtccgcttgccggtggccggcctgactcgttagccgccgtcccgtccggggcgaaaggaagcatacctcgctcactccatttggaggtaatggcttcacccaatctttcttgaatcaccattcctctctggatggatgttcttcaccaaacaatgctgtattgccgctcaatgtagcatcgtttcttgcgctaagatcttgaatcttgcttggagttgagagctggggattcatggtttattgttccgttactgtgcagcttcccccatccgcacgcgcaccagctgttcgacgaaatgccaccgcgcgctctgcccccagtggaggacgacgccggcagggactggctcggtgacctccccgaggaggtcctccaccacatcatgtccttcctagacgcgcgccaggccgtgcgaacctgtgtgctgtcgcggcggtggcgcaacctctggcgtaccgtgccttgcatcaacgctgacttcgatgagtttgatctcgtcttttaccaaggggacgacgaggactatgatgacgtgctggcgttcaaaaggtttgttaaccgtctgctggagctccgcgatcccaccgccatgatagacaccttctggcttaggtacaccaccagaccagaaggaaacacgaattccaatgaagatgcctacgggtggattagccacgctttgcagaagcaggcccgggttttagaggttgtcgtgttctgctgcttatttgagctggaccactcggtgtttacttcctgctacctgagaagaattgcgttctccggtattgttttatgcaagggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaaggtatgcatatttctattacatagtatgcatctttatttaggtgtcaatttgaaaaacttaaagttaagcatggtgttgtttcttcttgctacttcataattgcaattgtttaattacctgtacctgcaaaacttatgggcattgtttggataatgggatagggaaatgatttcccaactatcaaaaggtatctttaaatcctaaccatttattctccctcttccatttaatcctaacccttcattcatttcctaatcccaatcccacccttcatttcccattatccaaacacacccatgattcaagtgatcattttgtcgtgaaatcatgatgtgttgatacgatgaccactgatgttatcaaccactcaatgcaatatagttcatccactccagccattttttgtacacaaatcaatccgttgaacacttgtcctgctgtttctttgttggtagttttatctcaaaatgaaactggatcatttctcatgtgaggactagaatgttctagtctggatgcaatatcagggctgccaaccaaatgtgcatcttgtttgacatctgactgattattttagttgacatcagttcacatcttaccttactttttgaaagagggggttagcggtgagctggtgattgtaggttctagacccaaccctagtccagatttttcttggctgcagacatgtatgcattttatttctaagatatttatagaaaacaatattgcaattgcatatgcggccatttggtattgtgtaatcatgcatgaaatcgttggtttccggattaattatttattgcattttatattttagacagtatgttgaattgggatatgatgcaacgcacagacattatgcatttatgctacataaatttgaaaattaagagacattcgaaggcaatatgcaaacagaaagtgcttcaggattagttgaaaatctcacttctagtgcttcatcatgaaatatttgtactgttctttttactagtttttgttacatgacttgcatggaaagtatcctagcagtaagttaacatgcctgagactcgcattgaaaatatgaacatgtataatattgtttagtctggtcttttgttgacataatttcagtttctcctgtggtcttttaccatccgttatcctaaagttgaagctattatacatgcactccaaatagaattctttatcacatattgttaatgctctcaatttagttgccatattttctgattatattcatttgaatctttcgaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaaggtaaatgctttcgtccaaatgtgcactgatcctacttgattgctttttacacaagagttttcacatcgggatatagatcacctgtttttgttgctgtagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatgaaatggcaaaggatcacgaattagtttgccatcccattgaagaaaatcactctctttcgcctgtcggctacatctgctggttacagtagagtgttcagtgatcttgtttggtgctttctgctatgacgtgaaggtgagtagaaacaattgcatcttgccttgcatgtaaggtcagtggtattgtacttgtttgctgcgatcacatgatgatttgtagcaataatgatgctccaagaaaaatactgtatggctatggcgtctcctgatacagcctgccactgctttctcaacttggctggtctccggtgaaacgtattgtatttgtctctctcctaatataatcccatgttatagtgttgt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=180523 Os10g0127900 2014-06-08T02:40:21Z <p>Jieomlong: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> <br /> ===Expression===<br /> 1.OsFbx352 encodes a F-box domain protein and is suppressed by glucose<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.[[File:1.jpg]]<br /> Fig. 1. OsFbx352 contains a conserved F-box domain.(A) Alignment of the conserved F-box domain in OsFbx352 withother representative F-box-containing proteins: blue indicates residues identical to the OsFbx352 sequence; other colours indicate similar amino acids. The sequence at the bottom is the consensus sequence. (B)Phylogenetic tree analysis of the conserved F-box domain in OsFbx352 with other known F-box proteins from diverse organisms. Alignment and phylogenetic<br /> analysis were performed using clustal x 2 and mega 5, respectively. Approximately 40 amino acids that constitute the<br /> F-box motif in OsFBX352 were aligned with comparable regions from reported F-box proteins in humans (SKP2, BAB87202.1), yeast (CDC4, NP_116585.1), Arabidopsis (COI1, NP_565919; SON1, NP_179323; UFO, NP_564368.1; TIR1, NP_567135.1), and rice (OsMAIF, NP_001047698.1; OsDRF, NP_001052823.1).<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaactttcaagtccaaatttaaaactctcagttcatcttttaaaattttaattcaaccagtctgttcgttattttgctataaataatcacgcttatgctaatctaatcgcgtttagcgctaagcgaggggaggggcgcgtctgcgtgtggcatggcgccgtgcgcgcgagttactgtagcagcgcggatgcgcgagttacgagagaagcggaaaactgaaaacgcgcgagatctcgcgtattactccttgtcacggacagtttgagagcgggaggcctcctcccaatctccccccttccccttccatagttccatggtggcgctcttcagacgcgagcgcgaggagcaccgggtccgcttgccggtggccggcctgactcgttagccgccgtcccgtccggggcgaaaggaagcatacctcgctcactccatttggaggtaatggcttcacccaatctttcttgaatcaccattcctctctggatggatgttcttcaccaaacaatgctgtattgccgctcaatgtagcatcgtttcttgcgctaagatcttgaatcttgcttggagttgagagctggggattcatggtttattgttccgttactgtgcagcttcccccatccgcacgcgcaccagctgttcgacgaaatgccaccgcgcgctctgcccccagtggaggacgacgccggcagggactggctcggtgacctccccgaggaggtcctccaccacatcatgtccttcctagacgcgcgccaggccgtgcgaacctgtgtgctgtcgcggcggtggcgcaacctctggcgtaccgtgccttgcatcaacgctgacttcgatgagtttgatctcgtcttttaccaaggggacgacgaggactatgatgacgtgctggcgttcaaaaggtttgttaaccgtctgctggagctccgcgatcccaccgccatgatagacaccttctggcttaggtacaccaccagaccagaaggaaacacgaattccaatgaagatgcctacgggtggattagccacgctttgcagaagcaggcccgggttttagaggttgtcgtgttctgctgcttatttgagctggaccactcggtgtttacttcctgctacctgagaagaattgcgttctccggtattgttttatgcaagggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaaggtatgcatatttctattacatagtatgcatctttatttaggtgtcaatttgaaaaacttaaagttaagcatggtgttgtttcttcttgctacttcataattgcaattgtttaattacctgtacctgcaaaacttatgggcattgtttggataatgggatagggaaatgatttcccaactatcaaaaggtatctttaaatcctaaccatttattctccctcttccatttaatcctaacccttcattcatttcctaatcccaatcccacccttcatttcccattatccaaacacacccatgattcaagtgatcattttgtcgtgaaatcatgatgtgttgatacgatgaccactgatgttatcaaccactcaatgcaatatagttcatccactccagccattttttgtacacaaatcaatccgttgaacacttgtcctgctgtttctttgttggtagttttatctcaaaatgaaactggatcatttctcatgtgaggactagaatgttctagtctggatgcaatatcagggctgccaaccaaatgtgcatcttgtttgacatctgactgattattttagttgacatcagttcacatcttaccttactttttgaaagagggggttagcggtgagctggtgattgtaggttctagacccaaccctagtccagatttttcttggctgcagacatgtatgcattttatttctaagatatttatagaaaacaatattgcaattgcatatgcggccatttggtattgtgtaatcatgcatgaaatcgttggtttccggattaattatttattgcattttatattttagacagtatgttgaattgggatatgatgcaacgcacagacattatgcatttatgctacataaatttgaaaattaagagacattcgaaggcaatatgcaaacagaaagtgcttcaggattagttgaaaatctcacttctagtgcttcatcatgaaatatttgtactgttctttttactagtttttgttacatgacttgcatggaaagtatcctagcagtaagttaacatgcctgagactcgcattgaaaatatgaacatgtataatattgtttagtctggtcttttgttgacataatttcagtttctcctgtggtcttttaccatccgttatcctaaagttgaagctattatacatgcactccaaatagaattctttatcacatattgttaatgctctcaatttagttgccatattttctgattatattcatttgaatctttcgaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaaggtaaatgctttcgtccaaatgtgcactgatcctacttgattgctttttacacaagagttttcacatcgggatatagatcacctgtttttgttgctgtagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatgaaatggcaaaggatcacgaattagtttgccatcccattgaagaaaatcactctctttcgcctgtcggctacatctgctggttacagtagagtgttcagtgatcttgtttggtgctttctgctatgacgtgaaggtgagtagaaacaattgcatcttgccttgcatgtaaggtcagtggtattgtacttgtttgctgcgatcacatgatgatttgtagcaataatgatgctccaagaaaaatactgtatggctatggcgtctcctgatacagcctgccactgctttctcaacttggctggtctccggtgaaacgtattgtatttgtctctctcctaatataatcccatgttatagtgttgt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=180522 Os10g0127900 2014-06-08T02:39:57Z <p>Jieomlong: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> <br /> ===Expression===<br /> 1.OsFbx352 encodes a F-box domain protein and is suppressed by glucose<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.<br /> Fig. 1. OsFbx352 contains a conserved F-box domain.(A) Alignment of the conserved F-box domain in OsFbx352 withother representative F-box-containing proteins: blue indicates residues identical to the OsFbx352 sequence; other colours indicate similar amino acids. The sequence at the bottom is the consensus sequence. (B)Phylogenetic tree analysis of the conserved F-box domain in OsFbx352 with other known F-box proteins from diverse organisms. Alignment and phylogenetic<br /> analysis were performed using clustal x 2 and mega 5, respectively. Approximately 40 amino acids that constitute the<br /> F-box motif in OsFBX352 were aligned with comparable regions from reported F-box proteins in humans (SKP2, BAB87202.1), yeast (CDC4, NP_116585.1), Arabidopsis (COI1, NP_565919; SON1, NP_179323; UFO, NP_564368.1; TIR1, NP_567135.1), and rice (OsMAIF, NP_001047698.1; OsDRF, NP_001052823.1).<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaactttcaagtccaaatttaaaactctcagttcatcttttaaaattttaattcaaccagtctgttcgttattttgctataaataatcacgcttatgctaatctaatcgcgtttagcgctaagcgaggggaggggcgcgtctgcgtgtggcatggcgccgtgcgcgcgagttactgtagcagcgcggatgcgcgagttacgagagaagcggaaaactgaaaacgcgcgagatctcgcgtattactccttgtcacggacagtttgagagcgggaggcctcctcccaatctccccccttccccttccatagttccatggtggcgctcttcagacgcgagcgcgaggagcaccgggtccgcttgccggtggccggcctgactcgttagccgccgtcccgtccggggcgaaaggaagcatacctcgctcactccatttggaggtaatggcttcacccaatctttcttgaatcaccattcctctctggatggatgttcttcaccaaacaatgctgtattgccgctcaatgtagcatcgtttcttgcgctaagatcttgaatcttgcttggagttgagagctggggattcatggtttattgttccgttactgtgcagcttcccccatccgcacgcgcaccagctgttcgacgaaatgccaccgcgcgctctgcccccagtggaggacgacgccggcagggactggctcggtgacctccccgaggaggtcctccaccacatcatgtccttcctagacgcgcgccaggccgtgcgaacctgtgtgctgtcgcggcggtggcgcaacctctggcgtaccgtgccttgcatcaacgctgacttcgatgagtttgatctcgtcttttaccaaggggacgacgaggactatgatgacgtgctggcgttcaaaaggtttgttaaccgtctgctggagctccgcgatcccaccgccatgatagacaccttctggcttaggtacaccaccagaccagaaggaaacacgaattccaatgaagatgcctacgggtggattagccacgctttgcagaagcaggcccgggttttagaggttgtcgtgttctgctgcttatttgagctggaccactcggtgtttacttcctgctacctgagaagaattgcgttctccggtattgttttatgcaagggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaaggtatgcatatttctattacatagtatgcatctttatttaggtgtcaatttgaaaaacttaaagttaagcatggtgttgtttcttcttgctacttcataattgcaattgtttaattacctgtacctgcaaaacttatgggcattgtttggataatgggatagggaaatgatttcccaactatcaaaaggtatctttaaatcctaaccatttattctccctcttccatttaatcctaacccttcattcatttcctaatcccaatcccacccttcatttcccattatccaaacacacccatgattcaagtgatcattttgtcgtgaaatcatgatgtgttgatacgatgaccactgatgttatcaaccactcaatgcaatatagttcatccactccagccattttttgtacacaaatcaatccgttgaacacttgtcctgctgtttctttgttggtagttttatctcaaaatgaaactggatcatttctcatgtgaggactagaatgttctagtctggatgcaatatcagggctgccaaccaaatgtgcatcttgtttgacatctgactgattattttagttgacatcagttcacatcttaccttactttttgaaagagggggttagcggtgagctggtgattgtaggttctagacccaaccctagtccagatttttcttggctgcagacatgtatgcattttatttctaagatatttatagaaaacaatattgcaattgcatatgcggccatttggtattgtgtaatcatgcatgaaatcgttggtttccggattaattatttattgcattttatattttagacagtatgttgaattgggatatgatgcaacgcacagacattatgcatttatgctacataaatttgaaaattaagagacattcgaaggcaatatgcaaacagaaagtgcttcaggattagttgaaaatctcacttctagtgcttcatcatgaaatatttgtactgttctttttactagtttttgttacatgacttgcatggaaagtatcctagcagtaagttaacatgcctgagactcgcattgaaaatatgaacatgtataatattgtttagtctggtcttttgttgacataatttcagtttctcctgtggtcttttaccatccgttatcctaaagttgaagctattatacatgcactccaaatagaattctttatcacatattgttaatgctctcaatttagttgccatattttctgattatattcatttgaatctttcgaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaaggtaaatgctttcgtccaaatgtgcactgatcctacttgattgctttttacacaagagttttcacatcgggatatagatcacctgtttttgttgctgtagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatgaaatggcaaaggatcacgaattagtttgccatcccattgaagaaaatcactctctttcgcctgtcggctacatctgctggttacagtagagtgttcagtgatcttgtttggtgctttctgctatgacgtgaaggtgagtagaaacaattgcatcttgccttgcatgtaaggtcagtggtattgtacttgtttgctgcgatcacatgatgatttgtagcaataatgatgctccaagaaaaatactgtatggctatggcgtctcctgatacagcctgccactgctttctcaacttggctggtctccggtgaaacgtattgtatttgtctctctcctaatataatcccatgttatagtgttgt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:1.gif&diff=180517 File:1.gif 2014-06-08T02:39:00Z <p>Jieomlong: </p> <hr /> <div></div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=179212 Os10g0127900 2014-06-06T13:02:09Z <p>Jieomlong: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaactttcaagtccaaatttaaaactctcagttcatcttttaaaattttaattcaaccagtctgttcgttattttgctataaataatcacgcttatgctaatctaatcgcgtttagcgctaagcgaggggaggggcgcgtctgcgtgtggcatggcgccgtgcgcgcgagttactgtagcagcgcggatgcgcgagttacgagagaagcggaaaactgaaaacgcgcgagatctcgcgtattactccttgtcacggacagtttgagagcgggaggcctcctcccaatctccccccttccccttccatagttccatggtggcgctcttcagacgcgagcgcgaggagcaccgggtccgcttgccggtggccggcctgactcgttagccgccgtcccgtccggggcgaaaggaagcatacctcgctcactccatttggaggtaatggcttcacccaatctttcttgaatcaccattcctctctggatggatgttcttcaccaaacaatgctgtattgccgctcaatgtagcatcgtttcttgcgctaagatcttgaatcttgcttggagttgagagctggggattcatggtttattgttccgttactgtgcagcttcccccatccgcacgcgcaccagctgttcgacgaaatgccaccgcgcgctctgcccccagtggaggacgacgccggcagggactggctcggtgacctccccgaggaggtcctccaccacatcatgtccttcctagacgcgcgccaggccgtgcgaacctgtgtgctgtcgcggcggtggcgcaacctctggcgtaccgtgccttgcatcaacgctgacttcgatgagtttgatctcgtcttttaccaaggggacgacgaggactatgatgacgtgctggcgttcaaaaggtttgttaaccgtctgctggagctccgcgatcccaccgccatgatagacaccttctggcttaggtacaccaccagaccagaaggaaacacgaattccaatgaagatgcctacgggtggattagccacgctttgcagaagcaggcccgggttttagaggttgtcgtgttctgctgcttatttgagctggaccactcggtgtttacttcctgctacctgagaagaattgcgttctccggtattgttttatgcaagggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaaggtatgcatatttctattacatagtatgcatctttatttaggtgtcaatttgaaaaacttaaagttaagcatggtgttgtttcttcttgctacttcataattgcaattgtttaattacctgtacctgcaaaacttatgggcattgtttggataatgggatagggaaatgatttcccaactatcaaaaggtatctttaaatcctaaccatttattctccctcttccatttaatcctaacccttcattcatttcctaatcccaatcccacccttcatttcccattatccaaacacacccatgattcaagtgatcattttgtcgtgaaatcatgatgtgttgatacgatgaccactgatgttatcaaccactcaatgcaatatagttcatccactccagccattttttgtacacaaatcaatccgttgaacacttgtcctgctgtttctttgttggtagttttatctcaaaatgaaactggatcatttctcatgtgaggactagaatgttctagtctggatgcaatatcagggctgccaaccaaatgtgcatcttgtttgacatctgactgattattttagttgacatcagttcacatcttaccttactttttgaaagagggggttagcggtgagctggtgattgtaggttctagacccaaccctagtccagatttttcttggctgcagacatgtatgcattttatttctaagatatttatagaaaacaatattgcaattgcatatgcggccatttggtattgtgtaatcatgcatgaaatcgttggtttccggattaattatttattgcattttatattttagacagtatgttgaattgggatatgatgcaacgcacagacattatgcatttatgctacataaatttgaaaattaagagacattcgaaggcaatatgcaaacagaaagtgcttcaggattagttgaaaatctcacttctagtgcttcatcatgaaatatttgtactgttctttttactagtttttgttacatgacttgcatggaaagtatcctagcagtaagttaacatgcctgagactcgcattgaaaatatgaacatgtataatattgtttagtctggtcttttgttgacataatttcagtttctcctgtggtcttttaccatccgttatcctaaagttgaagctattatacatgcactccaaatagaattctttatcacatattgttaatgctctcaatttagttgccatattttctgattatattcatttgaatctttcgaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaaggtaaatgctttcgtccaaatgtgcactgatcctacttgattgctttttacacaagagttttcacatcgggatatagatcacctgtttttgttgctgtagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatgaaatggcaaaggatcacgaattagtttgccatcccattgaagaaaatcactctctttcgcctgtcggctacatctgctggttacagtagagtgttcagtgatcttgtttggtgctttctgctatgacgtgaaggtgagtagaaacaattgcatcttgccttgcatgtaaggtcagtggtattgtacttgtttgctgcgatcacatgatgatttgtagcaataatgatgctccaagaaaaatactgtatggctatggcgtctcctgatacagcctgccactgctttctcaacttggctggtctccggtgaaacgtattgtatttgtctctctcctaatataatcccatgttatagtgttgt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=179211 Os10g0127900 2014-06-06T13:01:28Z <p>Jieomlong: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> [[File:Example.jpg]]===Function===<br /> Please input function information here.<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaactttcaagtccaaatttaaaactctcagttcatcttttaaaattttaattcaaccagtctgttcgttattttgctataaataatcacgcttatgctaatctaatcgcgtttagcgctaagcgaggggaggggcgcgtctgcgtgtggcatggcgccgtgcgcgcgagttactgtagcagcgcggatgcgcgagttacgagagaagcggaaaactgaaaacgcgcgagatctcgcgtattactccttgtcacggacagtttgagagcgggaggcctcctcccaatctccccccttccccttccatagttccatggtggcgctcttcagacgcgagcgcgaggagcaccgggtccgcttgccggtggccggcctgactcgttagccgccgtcccgtccggggcgaaaggaagcatacctcgctcactccatttggaggtaatggcttcacccaatctttcttgaatcaccattcctctctggatggatgttcttcaccaaacaatgctgtattgccgctcaatgtagcatcgtttcttgcgctaagatcttgaatcttgcttggagttgagagctggggattcatggtttattgttccgttactgtgcagcttcccccatccgcacgcgcaccagctgttcgacgaaatgccaccgcgcgctctgcccccagtggaggacgacgccggcagggactggctcggtgacctccccgaggaggtcctccaccacatcatgtccttcctagacgcgcgccaggccgtgcgaacctgtgtgctgtcgcggcggtggcgcaacctctggcgtaccgtgccttgcatcaacgctgacttcgatgagtttgatctcgtcttttaccaaggggacgacgaggactatgatgacgtgctggcgttcaaaaggtttgttaaccgtctgctggagctccgcgatcccaccgccatgatagacaccttctggcttaggtacaccaccagaccagaaggaaacacgaattccaatgaagatgcctacgggtggattagccacgctttgcagaagcaggcccgggttttagaggttgtcgtgttctgctgcttatttgagctggaccactcggtgtttacttcctgctacctgagaagaattgcgttctccggtattgttttatgcaagggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaaggtatgcatatttctattacatagtatgcatctttatttaggtgtcaatttgaaaaacttaaagttaagcatggtgttgtttcttcttgctacttcataattgcaattgtttaattacctgtacctgcaaaacttatgggcattgtttggataatgggatagggaaatgatttcccaactatcaaaaggtatctttaaatcctaaccatttattctccctcttccatttaatcctaacccttcattcatttcctaatcccaatcccacccttcatttcccattatccaaacacacccatgattcaagtgatcattttgtcgtgaaatcatgatgtgttgatacgatgaccactgatgttatcaaccactcaatgcaatatagttcatccactccagccattttttgtacacaaatcaatccgttgaacacttgtcctgctgtttctttgttggtagttttatctcaaaatgaaactggatcatttctcatgtgaggactagaatgttctagtctggatgcaatatcagggctgccaaccaaatgtgcatcttgtttgacatctgactgattattttagttgacatcagttcacatcttaccttactttttgaaagagggggttagcggtgagctggtgattgtaggttctagacccaaccctagtccagatttttcttggctgcagacatgtatgcattttatttctaagatatttatagaaaacaatattgcaattgcatatgcggccatttggtattgtgtaatcatgcatgaaatcgttggtttccggattaattatttattgcattttatattttagacagtatgttgaattgggatatgatgcaacgcacagacattatgcatttatgctacataaatttgaaaattaagagacattcgaaggcaatatgcaaacagaaagtgcttcaggattagttgaaaatctcacttctagtgcttcatcatgaaatatttgtactgttctttttactagtttttgttacatgacttgcatggaaagtatcctagcagtaagttaacatgcctgagactcgcattgaaaatatgaacatgtataatattgtttagtctggtcttttgttgacataatttcagtttctcctgtggtcttttaccatccgttatcctaaagttgaagctattatacatgcactccaaatagaattctttatcacatattgttaatgctctcaatttagttgccatattttctgattatattcatttgaatctttcgaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaaggtaaatgctttcgtccaaatgtgcactgatcctacttgattgctttttacacaagagttttcacatcgggatatagatcacctgtttttgttgctgtagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatgaaatggcaaaggatcacgaattagtttgccatcccattgaagaaaatcactctctttcgcctgtcggctacatctgctggttacagtagagtgttcagtgatcttgtttggtgctttctgctatgacgtgaaggtgagtagaaacaattgcatcttgccttgcatgtaaggtcagtggtattgtacttgtttgctgcgatcacatgatgatttgtagcaataatgatgctccaagaaaaatactgtatggctatggcgtctcctgatacagcctgccactgctttctcaacttggctggtctccggtgaaacgtattgtatttgtctctctcctaatataatcccatgttatagtgttgt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=179209 Os10g0127900 2014-06-06T12:58:42Z <p>Jieomlong: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> [[File:Example.jpg]]===Function===<br /> Please input function information here.<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.[[File:Example.jpg]]<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaactttcaagtccaaatttaaaactctcagttcatcttttaaaattttaattcaaccagtctgttcgttattttgctataaataatcacgcttatgctaatctaatcgcgtttagcgctaagcgaggggaggggcgcgtctgcgtgtggcatggcgccgtgcgcgcgagttactgtagcagcgcggatgcgcgagttacgagagaagcggaaaactgaaaacgcgcgagatctcgcgtattactccttgtcacggacagtttgagagcgggaggcctcctcccaatctccccccttccccttccatagttccatggtggcgctcttcagacgcgagcgcgaggagcaccgggtccgcttgccggtggccggcctgactcgttagccgccgtcccgtccggggcgaaaggaagcatacctcgctcactccatttggaggtaatggcttcacccaatctttcttgaatcaccattcctctctggatggatgttcttcaccaaacaatgctgtattgccgctcaatgtagcatcgtttcttgcgctaagatcttgaatcttgcttggagttgagagctggggattcatggtttattgttccgttactgtgcagcttcccccatccgcacgcgcaccagctgttcgacgaaatgccaccgcgcgctctgcccccagtggaggacgacgccggcagggactggctcggtgacctccccgaggaggtcctccaccacatcatgtccttcctagacgcgcgccaggccgtgcgaacctgtgtgctgtcgcggcggtggcgcaacctctggcgtaccgtgccttgcatcaacgctgacttcgatgagtttgatctcgtcttttaccaaggggacgacgaggactatgatgacgtgctggcgttcaaaaggtttgttaaccgtctgctggagctccgcgatcccaccgccatgatagacaccttctggcttaggtacaccaccagaccagaaggaaacacgaattccaatgaagatgcctacgggtggattagccacgctttgcagaagcaggcccgggttttagaggttgtcgtgttctgctgcttatttgagctggaccactcggtgtttacttcctgctacctgagaagaattgcgttctccggtattgttttatgcaagggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaaggtatgcatatttctattacatagtatgcatctttatttaggtgtcaatttgaaaaacttaaagttaagcatggtgttgtttcttcttgctacttcataattgcaattgtttaattacctgtacctgcaaaacttatgggcattgtttggataatgggatagggaaatgatttcccaactatcaaaaggtatctttaaatcctaaccatttattctccctcttccatttaatcctaacccttcattcatttcctaatcccaatcccacccttcatttcccattatccaaacacacccatgattcaagtgatcattttgtcgtgaaatcatgatgtgttgatacgatgaccactgatgttatcaaccactcaatgcaatatagttcatccactccagccattttttgtacacaaatcaatccgttgaacacttgtcctgctgtttctttgttggtagttttatctcaaaatgaaactggatcatttctcatgtgaggactagaatgttctagtctggatgcaatatcagggctgccaaccaaatgtgcatcttgtttgacatctgactgattattttagttgacatcagttcacatcttaccttactttttgaaagagggggttagcggtgagctggtgattgtaggttctagacccaaccctagtccagatttttcttggctgcagacatgtatgcattttatttctaagatatttatagaaaacaatattgcaattgcatatgcggccatttggtattgtgtaatcatgcatgaaatcgttggtttccggattaattatttattgcattttatattttagacagtatgttgaattgggatatgatgcaacgcacagacattatgcatttatgctacataaatttgaaaattaagagacattcgaaggcaatatgcaaacagaaagtgcttcaggattagttgaaaatctcacttctagtgcttcatcatgaaatatttgtactgttctttttactagtttttgttacatgacttgcatggaaagtatcctagcagtaagttaacatgcctgagactcgcattgaaaatatgaacatgtataatattgtttagtctggtcttttgttgacataatttcagtttctcctgtggtcttttaccatccgttatcctaaagttgaagctattatacatgcactccaaatagaattctttatcacatattgttaatgctctcaatttagttgccatattttctgattatattcatttgaatctttcgaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaaggtaaatgctttcgtccaaatgtgcactgatcctacttgattgctttttacacaagagttttcacatcgggatatagatcacctgtttttgttgctgtagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatgaaatggcaaaggatcacgaattagtttgccatcccattgaagaaaatcactctctttcgcctgtcggctacatctgctggttacagtagagtgttcagtgatcttgtttggtgctttctgctatgacgtgaaggtgagtagaaacaattgcatcttgccttgcatgtaaggtcagtggtattgtacttgtttgctgcgatcacatgatgatttgtagcaataatgatgctccaagaaaaatactgtatggctatggcgtctcctgatacagcctgccactgctttctcaacttggctggtctccggtgaaacgtattgtatttgtctctctcctaatataatcccatgttatagtgttgt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=User:Jieomlong&diff=176721 User:Jieomlong 2014-06-03T11:32:42Z <p>Jieomlong: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> F-box proteins play diverse roles in regulating numerous physiological processes in plants. This study isolated a gene (OsFbx352) from rice encoding an F-box domain protein and characterized its role in seed germination. Expression of OsFbx352 was upregulated by abscisic acid (ABA). <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{IndicaGene|<br /> GeneName = BGIOSGA000063|<br /> Description = Putative uncharacterized protein|<br /> Definition = Oryza sativa Indica Group BGIOSGA000063, complete gene.|<br /> tag = P0483G10.23|<br /> tid = BGIOSGA000063-TA|<br /> pid = BGIOSGA000063-PA|<br /> Length = 2809|<br /> Source = Oryza sativa Indica Group<br /> <br /> ORGANISM Oryza sativa Indica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Indica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:46486990..46489798|<br /> CDS = 46489660..46489798,46489009..46489108,46488808..46488928,46488319..46488431,46488095..46488216&lt;br&gt;,46487646..46487805,46486990..46487110|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=IndicaChromosome01:46486990..46489798<br /> source=RiceIndica01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=IndicaChromosome01:46486990..46489798<br /> source=RiceIndica01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ATGGATGTGAGCAGGAGGAAGAAGTGGGTGGCGTGGGTGGCGGCGGTCACCATCTTCGTCGTGCTCATGCTCGTCACCCCGGCCATCCCGCAGAACGAGGAGTACCACGACTTCGCCGACCAGCGCCGCCTCTTCCTCGGGATCCCCAATACGCTGAATGTGATTTCAAACATCCCCTTCTTTTTTGTGGGTGTCGTTGGGCTCGTCTTGTGCCATTACAAAAACTACTTCAGGCTAAACTCGCAAGGGGAGCTCTGGAGTTGGACACTGTTCTTTGCTGGTGTGACTGCTGTTGCTTTTGGTTCATCCTATTATCATCTCAAACCGAATGATGCCACACTAGTTTGGGACAGATTGCCGATGACAATTGCTTTCACATCTGTCATGGCCATTTTCATTATTGAGAGGGTTGATGATAGGGCAGGAACAAAGTCCCTAGCACCTCTTGTTATTGCTGGTGCACTAAGCATCATGTTCTTTGACGATCTTCGCCCATATGCAGTAATTCAGTTTGTTCCATGCATTGCAATACCTGTGATGGCTATTGTAATTCCACCAATGTATACACATTCGAGCTATTGGCTGTGGGCAGCAGGATTTTACCTCCTTGCTAAGGTAGAAGAGGCCGCAGATAAACCTATATATAAATGGACCCATCAAATTGTTAGCGGTCATACTCTTAAGCATCTAGCTGCTGCAATGGTGCCTGTGTTCTTGGCACTCATGCTGGCCAAAAGGACTGTTGAACCAGAAAGGGTAAGTTTATTCCAGCGGTGGAAGGTCAAGTTGGTTGCAGTGAGAGAGACTCGATTCAACAAGGACAGGAATACAGTCGACTACAACTATATTGCAGTTTCCACTACACCTGAACCATAG&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MDVSRRKKWVAWVAAVTIFVVLMLVTPAIPQNEEYHDFADQRRLFLGIPNTLNVISNIPFFFVGVVGLVLCHYKNYFRLNSQGELWSWTLFFAGVTAVAFGSSYYHLKPNDATLVWDRLPMTIAFTSVMAIFIIERVDDRAGTKSLAPLVIAGALSIMFFDDLRPYAVIQFVPCIAIPVMAIVIPPMYTHSSYWLWAAGFYLLAKVEEAADKPIYKWTHQIVSGHTLKHLAAAMVPVFLALMLAKRTVEPERVSLFQRWKVKLVAVRETRFNKDRNTVDYNYIAVSTTPEP&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1..139#691..790#871..991#1368..1480#1583..1704#1994..2153#2689..2809#ATGGATGTGAGCAGGAGGAAGAAGTGGGTGGCGTGGGTGGCGGCGGTCACCATCTTCGTCGTGCTCATGCTCGTCACCCCGGCCATCCCGCAGAACGAGGAGTACCACGACTTCGCCGACCAGCGCCGCCTCTTCCTCGGTATGCCACAAACCAACCCTCCCTCCCCGCCGCAAACCTAGGGCGGACCTTCCCTTGCGGCCGCCATAGCTCGCCGTCCGCGGTATTGAGCTCCCGTCTCTCCGAGCTATTGAGCTCTCGGGGTAGCCTCCACCCGGCGCCGGCAAGACGATCCGCGAGCAGGAGAGCAGCGATTGTGCCAGTGCTTGTCCCTGACGGCGCAATGGCCAACGTTGAGTGACGAGTTGACTCGAAATCGCGAATTCGAGGTCGCTTGCATCGCCTAAATCGATCCCCTTTGTATTCACTACAAAAATTGATTGCCCTTTGTTTGCGCTGGGTTCAGCAAGATTAGCTTGTTAGGAAGGATTTTGCAGGCTATACCAGTTTCCTGTTTATTTCACGAACCCAAAATAAACATGAATATAGTATAATTGAGGGGGAATTTGAGGCGCAAACGAATAGAGTTTAAATTTTGCTTATATGTTCAATAAGAGGGTTGCAATGCTTGTAATCACTACTTGATGCACTAAGTTTGCAAAATCATGTGGATTTTTTTTTTTGTTTTGCAGGGATCCCCAATACGCTGAATGTGATTTCAAACATCCCCTTCTTTTTTGTGGGTGTCGTTGGGCTCGTCTTGTGCCATTACAAAAACTACTTCAGGCTAAAGTTAGACCATTACTGGTTCATCTTCTAATTCTAGTTAAGATTTTGATGCACTCTGTTGTTAACCTTCCCCTTCCTGGCAGCTCGCAAGGGGAGCTCTGGAGTTGGACACTGTTCTTTGCTGGTGTGACTGCTGTTGCTTTTGGTTCATCCTATTATCATCTCAAACCGAATGATGCCACACTAGTTTGGGACAGATTGCCGGTGAGTTGTTTCTAGACTTACATTGCCTTGCTCTAGGATTTGTACACACCCCATTATGTTGAAGCTGTCTCAGAATGTCAATGCATACATTTTTTTTGGTGGGATAGAATTCAACTCAGATCCTTGTGTAGTATAATCTGAATATGCTATGGCCCTGTTTAGATAGTGTTGTTCTGTTGGGTGTGATCTGCTTGAATCAAAACAGGGTAACTAATCCACTTGGATCTTTTGCCTGATACGCTCTAGTATATTTGGCTTGTTTTCTTCAGAATTGCCTGTCAGAGACATAAAGCATGTCATCAGTTCATAATTTTCACCCTTGCTTTTATCAATATTTAATATGTTAAAGGATGGCATATTTGCTTGTTCTATGTAGATGACAATTGCTTTCACATCTGTCATGGCCATTTTCATTATTGAGAGGGTTGATGATAGGGCAGGAACAAAGTCCCTAGCACCTCTTGTTATTGCTGGTGCACTAAGCATCATGTATTGGAGGCAAGCTTCTCCAATTGTTTAACCATCCTTCCTCAGAAATATTTTCTGTACTTCTTTCACACCTTCCATTTTGCTGCCTTAACTCTTATGTAGGTTCTTTGACGATCTTCGCCCATATGCAGTAATTCAGTTTGTTCCATGCATTGCAATACCTGTGATGGCTATTGTAATTCCACCAATGTATACACATTCGAGCTATTGGCTGTGGGCAGCAGGTTAGTGTTATTCTTGATCAATAGTATTTGATCTATGTACCTTCATTAGCATTTAGGTTGAAAAATGTGGTATTTGTGGTTCACCTATATATTATGAATCATTCAATTGATCATTTCTCTGCACCTAATAACTGTTGATGATACTTAAATGGTTTTCATTTTAATCTATTTCTACATATCATAAATTGATTCGCAGTGACCAAGTTAATTGATTGGTTTTCTGGAAATTCATACACAATCAGCACCTCACCATATTTTTCTGTTTTCTGATTTCTCAAAACAATTCCAGGATTTTACCTCCTTGCTAAGGTAGAAGAGGCCGCAGATAAACCTATATATAAATGGACCCATCAAATTGTTAGCGGTCATACTCTTAAGCATCTAGCTGCTGCAATGGTGCCTGTGTTCTTGGCACTCATGCTGGCCAAAAGGACTGTTGAACCAGAAAGGTATGCTTTATATACTGGCGTTTTAAGAACCACGTCTTTACTTCGATAAGTGCATCATTGGGTTCAACTCTTGAACTGTTCTTCTATTTAAGCTGTTGAATGGACCTTGGTCAGAATTCAGAAGATATACTTGCGTTGGCATTGTTACTTTGTCTTAAGGAATTATTCCATCATAAATTATTGAGCTATCTGTTCTATTAATTTTGCAAATGCAATCACTATTTGTTTTTGGGAAAAGAATGAAGTTTGTTTTCCTTCGGTTAGAATAAATTCATAAATGCCGTTGTAACTTTTGAATTAGTGTGTTGTTTGTTTCGCCAGTAATTCCATGGTGGTTCTCCCATAGTCCCATATCTTTGCTTCTTAGGATGAGCATATTTCAAATAATTTTATCCCCTCCATTGCAAACGATATCTGTTGTCTGTTGATTGATTTGTCAGCCTTTTTTTTAGCTCTGTTAATATCATGATTAAATTTATGAAGTCTTGCATAACTCTACCCCGTGTCTCATGATATTTTTTTTTCTGGAATGCAGGGTAAGTTTATTCCAGCGGTGGAAGGTCAAGTTGGTTGCAGTGAGAGAGACTCGATTCAACAAGGACAGGAATACAGTCGACTACAACTATATTGCAGTTTCCACTACACCTGAACCATAG&lt;/dnaseqindica&gt;|<br /> Link = [http://plants.ensembl.org/Oryza_indica/Gene/Summary?g=BGIOSGA000063 EnsemblPlants:BGIOSGA000063]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Indica mRNA]]<br /> [[Category:Oryza Sativa Indica Group]]<br /> [[Category:Indica Genes]]<br /> [[Category:Indica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=User:Jieomlong&diff=176720 User:Jieomlong 2014-06-03T11:32:25Z <p>Jieomlong: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> F-box proteins play diverse roles in regulating numerous physiological processes in plants. This study isolated a gene (OsFbx352) from rice encoding an F-box domain protein and characterized its role in seed germination. Expression of OsFbx352 was upregulated by abscisic acid (ABA). <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> 1.OsFbx352 encodes a F-box domain protein and is suppressed by glucose.<br /> 2.Generation of transgenic rice plants with altered.<br /> 3.Germination of transgenic seeds displayed different sensitivity to ABA expression of OsFbx352.<br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{IndicaGene|<br /> GeneName = BGIOSGA000063|<br /> Description = Putative uncharacterized protein|<br /> Definition = Oryza sativa Indica Group BGIOSGA000063, complete gene.|<br /> tag = P0483G10.23|<br /> tid = BGIOSGA000063-TA|<br /> pid = BGIOSGA000063-PA|<br /> Length = 2809|<br /> Source = Oryza sativa Indica Group<br /> <br /> ORGANISM Oryza sativa Indica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Indica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:46486990..46489798|<br /> CDS = 46489660..46489798,46489009..46489108,46488808..46488928,46488319..46488431,46488095..46488216&lt;br&gt;,46487646..46487805,46486990..46487110|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=IndicaChromosome01:46486990..46489798<br /> source=RiceIndica01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=IndicaChromosome01:46486990..46489798<br /> source=RiceIndica01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ATGGATGTGAGCAGGAGGAAGAAGTGGGTGGCGTGGGTGGCGGCGGTCACCATCTTCGTCGTGCTCATGCTCGTCACCCCGGCCATCCCGCAGAACGAGGAGTACCACGACTTCGCCGACCAGCGCCGCCTCTTCCTCGGGATCCCCAATACGCTGAATGTGATTTCAAACATCCCCTTCTTTTTTGTGGGTGTCGTTGGGCTCGTCTTGTGCCATTACAAAAACTACTTCAGGCTAAACTCGCAAGGGGAGCTCTGGAGTTGGACACTGTTCTTTGCTGGTGTGACTGCTGTTGCTTTTGGTTCATCCTATTATCATCTCAAACCGAATGATGCCACACTAGTTTGGGACAGATTGCCGATGACAATTGCTTTCACATCTGTCATGGCCATTTTCATTATTGAGAGGGTTGATGATAGGGCAGGAACAAAGTCCCTAGCACCTCTTGTTATTGCTGGTGCACTAAGCATCATGTTCTTTGACGATCTTCGCCCATATGCAGTAATTCAGTTTGTTCCATGCATTGCAATACCTGTGATGGCTATTGTAATTCCACCAATGTATACACATTCGAGCTATTGGCTGTGGGCAGCAGGATTTTACCTCCTTGCTAAGGTAGAAGAGGCCGCAGATAAACCTATATATAAATGGACCCATCAAATTGTTAGCGGTCATACTCTTAAGCATCTAGCTGCTGCAATGGTGCCTGTGTTCTTGGCACTCATGCTGGCCAAAAGGACTGTTGAACCAGAAAGGGTAAGTTTATTCCAGCGGTGGAAGGTCAAGTTGGTTGCAGTGAGAGAGACTCGATTCAACAAGGACAGGAATACAGTCGACTACAACTATATTGCAGTTTCCACTACACCTGAACCATAG&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MDVSRRKKWVAWVAAVTIFVVLMLVTPAIPQNEEYHDFADQRRLFLGIPNTLNVISNIPFFFVGVVGLVLCHYKNYFRLNSQGELWSWTLFFAGVTAVAFGSSYYHLKPNDATLVWDRLPMTIAFTSVMAIFIIERVDDRAGTKSLAPLVIAGALSIMFFDDLRPYAVIQFVPCIAIPVMAIVIPPMYTHSSYWLWAAGFYLLAKVEEAADKPIYKWTHQIVSGHTLKHLAAAMVPVFLALMLAKRTVEPERVSLFQRWKVKLVAVRETRFNKDRNTVDYNYIAVSTTPEP&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1..139#691..790#871..991#1368..1480#1583..1704#1994..2153#2689..2809#ATGGATGTGAGCAGGAGGAAGAAGTGGGTGGCGTGGGTGGCGGCGGTCACCATCTTCGTCGTGCTCATGCTCGTCACCCCGGCCATCCCGCAGAACGAGGAGTACCACGACTTCGCCGACCAGCGCCGCCTCTTCCTCGGTATGCCACAAACCAACCCTCCCTCCCCGCCGCAAACCTAGGGCGGACCTTCCCTTGCGGCCGCCATAGCTCGCCGTCCGCGGTATTGAGCTCCCGTCTCTCCGAGCTATTGAGCTCTCGGGGTAGCCTCCACCCGGCGCCGGCAAGACGATCCGCGAGCAGGAGAGCAGCGATTGTGCCAGTGCTTGTCCCTGACGGCGCAATGGCCAACGTTGAGTGACGAGTTGACTCGAAATCGCGAATTCGAGGTCGCTTGCATCGCCTAAATCGATCCCCTTTGTATTCACTACAAAAATTGATTGCCCTTTGTTTGCGCTGGGTTCAGCAAGATTAGCTTGTTAGGAAGGATTTTGCAGGCTATACCAGTTTCCTGTTTATTTCACGAACCCAAAATAAACATGAATATAGTATAATTGAGGGGGAATTTGAGGCGCAAACGAATAGAGTTTAAATTTTGCTTATATGTTCAATAAGAGGGTTGCAATGCTTGTAATCACTACTTGATGCACTAAGTTTGCAAAATCATGTGGATTTTTTTTTTTGTTTTGCAGGGATCCCCAATACGCTGAATGTGATTTCAAACATCCCCTTCTTTTTTGTGGGTGTCGTTGGGCTCGTCTTGTGCCATTACAAAAACTACTTCAGGCTAAAGTTAGACCATTACTGGTTCATCTTCTAATTCTAGTTAAGATTTTGATGCACTCTGTTGTTAACCTTCCCCTTCCTGGCAGCTCGCAAGGGGAGCTCTGGAGTTGGACACTGTTCTTTGCTGGTGTGACTGCTGTTGCTTTTGGTTCATCCTATTATCATCTCAAACCGAATGATGCCACACTAGTTTGGGACAGATTGCCGGTGAGTTGTTTCTAGACTTACATTGCCTTGCTCTAGGATTTGTACACACCCCATTATGTTGAAGCTGTCTCAGAATGTCAATGCATACATTTTTTTTGGTGGGATAGAATTCAACTCAGATCCTTGTGTAGTATAATCTGAATATGCTATGGCCCTGTTTAGATAGTGTTGTTCTGTTGGGTGTGATCTGCTTGAATCAAAACAGGGTAACTAATCCACTTGGATCTTTTGCCTGATACGCTCTAGTATATTTGGCTTGTTTTCTTCAGAATTGCCTGTCAGAGACATAAAGCATGTCATCAGTTCATAATTTTCACCCTTGCTTTTATCAATATTTAATATGTTAAAGGATGGCATATTTGCTTGTTCTATGTAGATGACAATTGCTTTCACATCTGTCATGGCCATTTTCATTATTGAGAGGGTTGATGATAGGGCAGGAACAAAGTCCCTAGCACCTCTTGTTATTGCTGGTGCACTAAGCATCATGTATTGGAGGCAAGCTTCTCCAATTGTTTAACCATCCTTCCTCAGAAATATTTTCTGTACTTCTTTCACACCTTCCATTTTGCTGCCTTAACTCTTATGTAGGTTCTTTGACGATCTTCGCCCATATGCAGTAATTCAGTTTGTTCCATGCATTGCAATACCTGTGATGGCTATTGTAATTCCACCAATGTATACACATTCGAGCTATTGGCTGTGGGCAGCAGGTTAGTGTTATTCTTGATCAATAGTATTTGATCTATGTACCTTCATTAGCATTTAGGTTGAAAAATGTGGTATTTGTGGTTCACCTATATATTATGAATCATTCAATTGATCATTTCTCTGCACCTAATAACTGTTGATGATACTTAAATGGTTTTCATTTTAATCTATTTCTACATATCATAAATTGATTCGCAGTGACCAAGTTAATTGATTGGTTTTCTGGAAATTCATACACAATCAGCACCTCACCATATTTTTCTGTTTTCTGATTTCTCAAAACAATTCCAGGATTTTACCTCCTTGCTAAGGTAGAAGAGGCCGCAGATAAACCTATATATAAATGGACCCATCAAATTGTTAGCGGTCATACTCTTAAGCATCTAGCTGCTGCAATGGTGCCTGTGTTCTTGGCACTCATGCTGGCCAAAAGGACTGTTGAACCAGAAAGGTATGCTTTATATACTGGCGTTTTAAGAACCACGTCTTTACTTCGATAAGTGCATCATTGGGTTCAACTCTTGAACTGTTCTTCTATTTAAGCTGTTGAATGGACCTTGGTCAGAATTCAGAAGATATACTTGCGTTGGCATTGTTACTTTGTCTTAAGGAATTATTCCATCATAAATTATTGAGCTATCTGTTCTATTAATTTTGCAAATGCAATCACTATTTGTTTTTGGGAAAAGAATGAAGTTTGTTTTCCTTCGGTTAGAATAAATTCATAAATGCCGTTGTAACTTTTGAATTAGTGTGTTGTTTGTTTCGCCAGTAATTCCATGGTGGTTCTCCCATAGTCCCATATCTTTGCTTCTTAGGATGAGCATATTTCAAATAATTTTATCCCCTCCATTGCAAACGATATCTGTTGTCTGTTGATTGATTTGTCAGCCTTTTTTTTAGCTCTGTTAATATCATGATTAAATTTATGAAGTCTTGCATAACTCTACCCCGTGTCTCATGATATTTTTTTTTCTGGAATGCAGGGTAAGTTTATTCCAGCGGTGGAAGGTCAAGTTGGTTGCAGTGAGAGAGACTCGATTCAACAAGGACAGGAATACAGTCGACTACAACTATATTGCAGTTTCCACTACACCTGAACCATAG&lt;/dnaseqindica&gt;|<br /> Link = [http://plants.ensembl.org/Oryza_indica/Gene/Summary?g=BGIOSGA000063 EnsemblPlants:BGIOSGA000063]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Indica mRNA]]<br /> [[Category:Oryza Sativa Indica Group]]<br /> [[Category:Indica Genes]]<br /> [[Category:Indica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=176717 Os10g0127900 2014-06-03T11:29:57Z <p>Jieomlong: </p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> ===Expression===<br /> Please input expression information here.<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaactttcaagtccaaatttaaaactctcagttcatcttttaaaattttaattcaaccagtctgttcgttattttgctataaataatcacgcttatgctaatctaatcgcgtttagcgctaagcgaggggaggggcgcgtctgcgtgtggcatggcgccgtgcgcgcgagttactgtagcagcgcggatgcgcgagttacgagagaagcggaaaactgaaaacgcgcgagatctcgcgtattactccttgtcacggacagtttgagagcgggaggcctcctcccaatctccccccttccccttccatagttccatggtggcgctcttcagacgcgagcgcgaggagcaccgggtccgcttgccggtggccggcctgactcgttagccgccgtcccgtccggggcgaaaggaagcatacctcgctcactccatttggaggtaatggcttcacccaatctttcttgaatcaccattcctctctggatggatgttcttcaccaaacaatgctgtattgccgctcaatgtagcatcgtttcttgcgctaagatcttgaatcttgcttggagttgagagctggggattcatggtttattgttccgttactgtgcagcttcccccatccgcacgcgcaccagctgttcgacgaaatgccaccgcgcgctctgcccccagtggaggacgacgccggcagggactggctcggtgacctccccgaggaggtcctccaccacatcatgtccttcctagacgcgcgccaggccgtgcgaacctgtgtgctgtcgcggcggtggcgcaacctctggcgtaccgtgccttgcatcaacgctgacttcgatgagtttgatctcgtcttttaccaaggggacgacgaggactatgatgacgtgctggcgttcaaaaggtttgttaaccgtctgctggagctccgcgatcccaccgccatgatagacaccttctggcttaggtacaccaccagaccagaaggaaacacgaattccaatgaagatgcctacgggtggattagccacgctttgcagaagcaggcccgggttttagaggttgtcgtgttctgctgcttatttgagctggaccactcggtgtttacttcctgctacctgagaagaattgcgttctccggtattgttttatgcaagggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaaggtatgcatatttctattacatagtatgcatctttatttaggtgtcaatttgaaaaacttaaagttaagcatggtgttgtttcttcttgctacttcataattgcaattgtttaattacctgtacctgcaaaacttatgggcattgtttggataatgggatagggaaatgatttcccaactatcaaaaggtatctttaaatcctaaccatttattctccctcttccatttaatcctaacccttcattcatttcctaatcccaatcccacccttcatttcccattatccaaacacacccatgattcaagtgatcattttgtcgtgaaatcatgatgtgttgatacgatgaccactgatgttatcaaccactcaatgcaatatagttcatccactccagccattttttgtacacaaatcaatccgttgaacacttgtcctgctgtttctttgttggtagttttatctcaaaatgaaactggatcatttctcatgtgaggactagaatgttctagtctggatgcaatatcagggctgccaaccaaatgtgcatcttgtttgacatctgactgattattttagttgacatcagttcacatcttaccttactttttgaaagagggggttagcggtgagctggtgattgtaggttctagacccaaccctagtccagatttttcttggctgcagacatgtatgcattttatttctaagatatttatagaaaacaatattgcaattgcatatgcggccatttggtattgtgtaatcatgcatgaaatcgttggtttccggattaattatttattgcattttatattttagacagtatgttgaattgggatatgatgcaacgcacagacattatgcatttatgctacataaatttgaaaattaagagacattcgaaggcaatatgcaaacagaaagtgcttcaggattagttgaaaatctcacttctagtgcttcatcatgaaatatttgtactgttctttttactagtttttgttacatgacttgcatggaaagtatcctagcagtaagttaacatgcctgagactcgcattgaaaatatgaacatgtataatattgtttagtctggtcttttgttgacataatttcagtttctcctgtggtcttttaccatccgttatcctaaagttgaagctattatacatgcactccaaatagaattctttatcacatattgttaatgctctcaatttagttgccatattttctgattatattcatttgaatctttcgaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaaggtaaatgctttcgtccaaatgtgcactgatcctacttgattgctttttacacaagagttttcacatcgggatatagatcacctgtttttgttgctgtagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatgaaatggcaaaggatcacgaattagtttgccatcccattgaagaaaatcactctctttcgcctgtcggctacatctgctggttacagtagagtgttcagtgatcttgtttggtgctttctgctatgacgtgaaggtgagtagaaacaattgcatcttgccttgcatgtaaggtcagtggtattgtacttgtttgctgcgatcacatgatgatttgtagcaataatgatgctccaagaaaaatactgtatggctatggcgtctcctgatacagcctgccactgctttctcaacttggctggtctccggtgaaacgtattgtatttgtctctctcctaatataatcccatgttatagtgttgt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=176715 Os10g0127900 2014-06-03T11:27:02Z <p>Jieomlong: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> ===Expression===<br /> Please input expression information here.<br /> A database search with the predicted polypeptide sequence showed that the F-box domain of OsFBX352 was related to those of the previously identified F-box proteins, including Arabidopsis SON (70% similarity), yeast CDC4 (65% similarity), rice OsDRF (67.5% similarity), and human SKP2 (57.5% similarity) (Fig. 1A). Phylogenetic analysis also revealed that OsFBX352 was closely related to Arabidopsis SON1 and UFO and to rice MAIF (Fig. 1B). These results suggest that OsFBX352 may be involved in the formation of E3 ubiquitin ligase complex.[[File:Example.jpg]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaactttcaagtccaaatttaaaactctcagttcatcttttaaaattttaattcaaccagtctgttcgttattttgctataaataatcacgcttatgctaatctaatcgcgtttagcgctaagcgaggggaggggcgcgtctgcgtgtggcatggcgccgtgcgcgcgagttactgtagcagcgcggatgcgcgagttacgagagaagcggaaaactgaaaacgcgcgagatctcgcgtattactccttgtcacggacagtttgagagcgggaggcctcctcccaatctccccccttccccttccatagttccatggtggcgctcttcagacgcgagcgcgaggagcaccgggtccgcttgccggtggccggcctgactcgttagccgccgtcccgtccggggcgaaaggaagcatacctcgctcactccatttggaggtaatggcttcacccaatctttcttgaatcaccattcctctctggatggatgttcttcaccaaacaatgctgtattgccgctcaatgtagcatcgtttcttgcgctaagatcttgaatcttgcttggagttgagagctggggattcatggtttattgttccgttactgtgcagcttcccccatccgcacgcgcaccagctgttcgacgaaatgccaccgcgcgctctgcccccagtggaggacgacgccggcagggactggctcggtgacctccccgaggaggtcctccaccacatcatgtccttcctagacgcgcgccaggccgtgcgaacctgtgtgctgtcgcggcggtggcgcaacctctggcgtaccgtgccttgcatcaacgctgacttcgatgagtttgatctcgtcttttaccaaggggacgacgaggactatgatgacgtgctggcgttcaaaaggtttgttaaccgtctgctggagctccgcgatcccaccgccatgatagacaccttctggcttaggtacaccaccagaccagaaggaaacacgaattccaatgaagatgcctacgggtggattagccacgctttgcagaagcaggcccgggttttagaggttgtcgtgttctgctgcttatttgagctggaccactcggtgtttacttcctgctacctgagaagaattgcgttctccggtattgttttatgcaagggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaaggtatgcatatttctattacatagtatgcatctttatttaggtgtcaatttgaaaaacttaaagttaagcatggtgttgtttcttcttgctacttcataattgcaattgtttaattacctgtacctgcaaaacttatgggcattgtttggataatgggatagggaaatgatttcccaactatcaaaaggtatctttaaatcctaaccatttattctccctcttccatttaatcctaacccttcattcatttcctaatcccaatcccacccttcatttcccattatccaaacacacccatgattcaagtgatcattttgtcgtgaaatcatgatgtgttgatacgatgaccactgatgttatcaaccactcaatgcaatatagttcatccactccagccattttttgtacacaaatcaatccgttgaacacttgtcctgctgtttctttgttggtagttttatctcaaaatgaaactggatcatttctcatgtgaggactagaatgttctagtctggatgcaatatcagggctgccaaccaaatgtgcatcttgtttgacatctgactgattattttagttgacatcagttcacatcttaccttactttttgaaagagggggttagcggtgagctggtgattgtaggttctagacccaaccctagtccagatttttcttggctgcagacatgtatgcattttatttctaagatatttatagaaaacaatattgcaattgcatatgcggccatttggtattgtgtaatcatgcatgaaatcgttggtttccggattaattatttattgcattttatattttagacagtatgttgaattgggatatgatgcaacgcacagacattatgcatttatgctacataaatttgaaaattaagagacattcgaaggcaatatgcaaacagaaagtgcttcaggattagttgaaaatctcacttctagtgcttcatcatgaaatatttgtactgttctttttactagtttttgttacatgacttgcatggaaagtatcctagcagtaagttaacatgcctgagactcgcattgaaaatatgaacatgtataatattgtttagtctggtcttttgttgacataatttcagtttctcctgtggtcttttaccatccgttatcctaaagttgaagctattatacatgcactccaaatagaattctttatcacatattgttaatgctctcaatttagttgccatattttctgattatattcatttgaatctttcgaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaaggtaaatgctttcgtccaaatgtgcactgatcctacttgattgctttttacacaagagttttcacatcgggatatagatcacctgtttttgttgctgtagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatgaaatggcaaaggatcacgaattagtttgccatcccattgaagaaaatcactctctttcgcctgtcggctacatctgctggttacagtagagtgttcagtgatcttgtttggtgctttctgctatgacgtgaaggtgagtagaaacaattgcatcttgccttgcatgtaaggtcagtggtattgtacttgtttgctgcgatcacatgatgatttgtagcaataatgatgctccaagaaaaatactgtatggctatggcgtctcctgatacagcctgccactgctttctcaacttggctggtctccggtgaaacgtattgtatttgtctctctcctaatataatcccatgttatagtgttgt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0127900&diff=176701 Os10g0127900 2014-06-03T11:22:52Z <p>Jieomlong: </p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study identified an F-box gene, designed OsFbx352, from rice, and characterized its function during seed germination by generating transgenic rice plants with overexpression and knockdown of OsFbx352. More specifically, this study evaluated the role of OsFbx352 in seed germination with emphasis on the interactions<br /> among ABA, OsFbx352, and glucose. The results reveal that OsFbx352 was involved in the regulation of glucose-induced delay of seed germination by modulating ABA metabolism.<br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os10g0127900|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001070622.1 GI:115480987 GeneID:4348024|<br /> Length = 8580 bp|<br /> Definition = Oryza sativa Japonica Group Os10g0127900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|<br /> AP = Chromosome 10:1722588..1731167|<br /> CDS = 1722947..1723168,1723268..1723423,1724688..1725077,1730916..1731026|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008403:1722588..1731167<br /> source=RiceChromosome10<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcgggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;PLDIQKRRRAPYVAAVCPCPAPRRAPLRRVSPSRRLAGFFAQLE AGCPALEDLFLHQCGVHDDEISSHTLKVLTFDSVFFYMPMDTVEFTLLNKTSISLPSV TSLTISTPEGFTPILKDTASVVTASVSVSVTMSSFRFRFDANDLGQYLQSLSGITNLE FNYQGSKLTIENHLQWCPEFLNVVNLTLGQWCLDSNFYALIVFLQNSPRLQKLTLKLE KSNMRISRRIIGELTEISFTCEHLNTVEVICSENDPQVITVQDFFVSSGMTSVQFHIK HWSPYANDLPAFIRSI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;8000..8221#7745..7900#6091..6480#142..252#tgcatttcatcgaacaccttgcgatcaccctctcaccaccgctagagatcaccattgtgaggctccccggccggagctggaggttggacggagtggaggaagagccgtgctgccgccgtcgccatgggggaagccgggtagccgctggacatccagaaacgccgtcgagcaccttacgtcgccgccgtctgcccctgtcctgcaccgcgtcgagctccacttcgccgcgtttcgccatctcgccgtctcgcggtacaccctcgcgcctgtagcgtcctccatacatctaattgctagcgccggctccaaaatcggcgattagcgcccatggccgtgccgccccggccgccgtttcgtcgccggcgagctctccgtgggtaggctatcccgggagctcccctctccatatagttcgtgaaatcgaatccccataccccgtagatgctcccgcacccttcaattcggccggaggatgtccctagcaccgaagccccttgtagccgagctcgggcatggcgtcgcctgatggcgccgcgcgcccccgtctcctcgcgcgccggctgccattggcgggagattgccggagctcctccgcgcctcaccccggccgtcgccgagccgcccgccgccgtcccggctcgggccggccacgccgactgcgccgagccgcgcctctgcatcgctcggtaacgggcgagaggaagaagaagatgaggagagagagaaaccgtgtgggacccacatgtgattagcgcacagtaatcctatccctaacggtgattatcccactcaatggcaggtggggtccctatagttaaaatctaagaaaacacaattatatattttgttcctatgacaggcgggacccaatggtaattaaacaagcaagtttgaccctaagtcaaccatgccggtcccacatgtcaaccacactgggttttggataatgtttccgattaaaaataaatcatttatgaacagtgcatttttacaatttctcggattaactcaaatttgaatctttaaattagtataacttattcattttagctccgattcgagtgaaacttgaacctaaattcatctaaattcataagctttccaatggtatataactcattatttaataaaatatattaatatcaaatgattagattatggtcaacttaaaaatgtgctaataaataaaattgatattgtacagtagagtagatgtcaaataaatatatttaacatgttttgtcactgtagcaaccacataatctaagtataagtgatgtctcaaatgaatggatgtataggaaaatactagtgcttatttaatattcgatggccatataattaaattcatgacttaaaaattagttacataaagtttagcttcatattttgtaactaaaatattaatactcatagataaatctttaacttgtttatgagaaataataacagagctgggtgtgatgaaatcaatcgaatgatagatttaaccagtgcaatatcctatatagtagtatatttataatttggttgacacctattatttaacatattatttaacagaacaataaataactcatgttcggtaaataatccaattaacatattattggacttgcatgtacagtacatccatttaacacatacacagtaagtcaatcacctactgttgcacctattaaactatgaattgtttgataaacatcaaagttagataaaaacactaattataaatctagacctcgttacgaggccacgtagacaaataatctgtacctataacctcacagatctatatagagcatggatgattattaacccttttataatttataatgacaagtaaaatatgatcgtgattaaaataatgccccattggtaaaataataatataaatgattcactgtaattatttgattgatcctaaatctatccaacaacggaacctctagtatatgaccattttatttagatagatgcatgtgtagacataatccttccatagccattagctagactaaaccaccatgacatcagaaagactaagaaaccttagctcttagcttgattagaatccattagcaaacgcaagtagtatgagcaaccctaggttcgacctcaacaattatattttgtttgcgcacattggattgttgtttgaacattgatttttctcgcgtattatagtattggtgtatcgattagtcgtgaggcaacgtatgcagcttccaggaggtgaacgttgatcaatattatatcaagaataagaactatcctaagcaggcaagtcatcaccccattccctgagcatgttgatcccaattgtgaaattgttttgtttacaaataaaattgcatgcagtgatgaatatcctacttgttgttatgccatgcctttgattattgcttatctcattattcccctgtacccataattatgtatgagtccctagtcacttatgacaaatgcttagtaatgctaatttagattcatgcattctcatatttatcaaatgctttatgcttgggaaactaccttggggaggtagttgagatacggcatgtggagacatgagtgccacattgccatgacattgatgagatgatttgtgaaagaaaatgaaaactgaacaaatgttttcgactagggcggctggaggacttgggtggtgtccggaaaaggctagtgccgtccctggttagttaaggaccgagccatgaagctaagcatgaaacgacccccgtacaaccgtacttctcgtatgggtatagacctagcagagtaaatagccgagcggaggtagtatccctgcatagtggttttcttgatgtgtgtgaggcagggcgtacggtggggcagccattggtaggaccacgaggggcaggtgcctacagtggtgtcgccatcggtaggactgccatatgtaaacctaaacttaactataacttaatgcatgtgtgagtcttcccttcccgggagagccagaactcctctcactgctagaaaccttggacgcctagagtgcatgaggactaaagttcatggagcgggtactgccaaagtgaggttattgaaaggctttgccatgacgcgtctcatgtgttgggatgaggctcatgtgttgggcagtcacggagtatgggtaaagtgtacttccactgcagtgtgagtaaaccaatctattcgaatagccgtgctcacggttattgagcaccgggacatgtattacacttggctagactctaaattcttaaaatgatgggtgggatgttgcatgatgattttgtactggtggaacaacttctcgagaggcgagaagaggtagaccccagaaaaccatgacgactagtggcgggaagctatccttgggaacacaatggatggtggacagaaccgtcgctgtttaatgaacaatggaattaaaacttgaccagtttatgctaggtcttaggtttgtgaaatgatatttgaaaactcagctttacgcaaagtaaccataaccactccttgtattatcctgtacattactgcatttttttgtggtggcttgctgagtacttttgtactcattgttgctctatatatatcttttagcagagtgttgaagagaagcccttgttagtacgcttgcgtacctaacaagatgatcggagtgtggttccattctaggtctcgttcccagtcgactgcttgtggcatgttaaccgggcccttgtattattttgtctttcgctgttgttctctgatagttatcggcctgcctggccctaatgtaagcatataactcttttagcctgaattcattcgtgatatgttgtgattcggctgtgtgtaccaactactgatccagggattggtactgataaacacagaagatttccgatttccaaaatcggggatcgacaattactaaatctaaatctatagttatgtaatacttcactttaaattagtagtcacgtgataaatttaatattaaacatgtagttttattatatatcatcttgaataaatatatagtcttgtaatagtttgatcataggtttattattttactcaaattttcaaaatgttaaaaaataagttccaacctaattgcataacttcatatcaaatcgctaaaagcaaaaaggaacacatggtatttgagcaagtactataaaatttctaaatactagtcataagataccaatcaagcttaaatggtaatatggaggagaggagagaagaaagaaatgagagtgaaatagagaagttaattggtgacaaatgcgttgaggttagtgtaatagaggtaatatattgtataggttatttttaaatttatgagaagatgacgtggataaaacttgcttgaaccaacttggggatggtgttttcaagcaggaatcctatctcgcgcagatgcgcgagcaaactaactcggatctactaggctcaccctaccctacactgcagttgttagcattttttttctctttttcgagtgatttcctctccttccgtcgttttcccgtgcaactttttgtctctcgtgattttttttctttttctcgcgcaattttttccttttttttttgcaagattattttttcccgtgtgctatcaggctaagtttcaacttcattttattttgaaaatttttgactcaaaatttaaaaacttctaacataaaattgaaaagttctaactcaaatttagaaattttcaactgaaaatttgaaaatttcgattaagatttgaaaactttcaactaagatttgaaactttcaatttaagatttgaaaaaattcaactcaaaattgaaaacttgcaaatcaaaatttgaaaactttcaaatctaatttgaaaactttaaagttaaaaattcaaaactttcaaatcaaatttgaaaactttcaactcaaaattttaaaaaaccatatccatttttttaaactttcaagtccaaatttaaaactctcagttcatcttttaaaattttaattcaaccagtctgttcgttattttgctataaataatcacgcttatgctaatctaatcgcgtttagcgctaagcgaggggaggggcgcgtctgcgtgtggcatggcgccgtgcgcgcgagttactgtagcagcgcggatgcgcgagttacgagagaagcggaaaactgaaaacgcgcgagatctcgcgtattactccttgtcacggacagtttgagagcgggaggcctcctcccaatctccccccttccccttccatagttccatggtggcgctcttcagacgcgagcgcgaggagcaccgggtccgcttgccggtggccggcctgactcgttagccgccgtcccgtccggggcgaaaggaagcatacctcgctcactccatttggaggtaatggcttcacccaatctttcttgaatcaccattcctctctggatggatgttcttcaccaaacaatgctgtattgccgctcaatgtagcatcgtttcttgcgctaagatcttgaatcttgcttggagttgagagctggggattcatggtttattgttccgttactgtgcagcttcccccatccgcacgcgcaccagctgttcgacgaaatgccaccgcgcgctctgcccccagtggaggacgacgccggcagggactggctcggtgacctccccgaggaggtcctccaccacatcatgtccttcctagacgcgcgccaggccgtgcgaacctgtgtgctgtcgcggcggtggcgcaacctctggcgtaccgtgccttgcatcaacgctgacttcgatgagtttgatctcgtcttttaccaaggggacgacgaggactatgatgacgtgctggcgttcaaaaggtttgttaaccgtctgctggagctccgcgatcccaccgccatgatagacaccttctggcttaggtacaccaccagaccagaaggaaacacgaattccaatgaagatgcctacgggtggattagccacgctttgcagaagcaggcccgggttttagaggttgtcgtgttctgctgcttatttgagctggaccactcggtgtttacttcctgctacctgagaagaattgcgttctccggtattgttttatgcaagggtttcttcgcacaacttgaagctggctgtccagcattggaagatctcttcttacatcaatgcggcgttcacgatgacgaaatctcctcccatacactgaaggttttgaccttcgatagtgtttttttctacatgccgatggacactgttgaattcaccttgctgaacaagacatctatttctcttccaagtgttacttctctgaccatatctactcccgagggtttcacacctatactaaaggacacggcatcagtagtgactgcatcagtatcagtatcagttacaatgtctagttttagatttcgttttgatgctaatgatctcggccagtatctccagagcctttccggtattacaaatttagagttcaactatcagggttcaaaggtatgcatatttctattacatagtatgcatctttatttaggtgtcaatttgaaaaacttaaagttaagcatggtgttgtttcttcttgctacttcataattgcaattgtttaattacctgtacctgcaaaacttatgggcattgtttggataatgggatagggaaatgatttcccaactatcaaaaggtatctttaaatcctaaccatttattctccctcttccatttaatcctaacccttcattcatttcctaatcccaatcccacccttcatttcccattatccaaacacacccatgattcaagtgatcattttgtcgtgaaatcatgatgtgttgatacgatgaccactgatgttatcaaccactcaatgcaatatagttcatccactccagccattttttgtacacaaatcaatccgttgaacacttgtcctgctgtttctttgttggtagttttatctcaaaatgaaactggatcatttctcatgtgaggactagaatgttctagtctggatgcaatatcagggctgccaaccaaatgtgcatcttgtttgacatctgactgattattttagttgacatcagttcacatcttaccttactttttgaaagagggggttagcggtgagctggtgattgtaggttctagacccaaccctagtccagatttttcttggctgcagacatgtatgcattttatttctaagatatttatagaaaacaatattgcaattgcatatgcggccatttggtattgtgtaatcatgcatgaaatcgttggtttccggattaattatttattgcattttatattttagacagtatgttgaattgggatatgatgcaacgcacagacattatgcatttatgctacataaatttgaaaattaagagacattcgaaggcaatatgcaaacagaaagtgcttcaggattagttgaaaatctcacttctagtgcttcatcatgaaatatttgtactgttctttttactagtttttgttacatgacttgcatggaaagtatcctagcagtaagttaacatgcctgagactcgcattgaaaatatgaacatgtataatattgtttagtctggtcttttgttgacataatttcagtttctcctgtggtcttttaccatccgttatcctaaagttgaagctattatacatgcactccaaatagaattctttatcacatattgttaatgctctcaatttagttgccatattttctgattatattcatttgaatctttcgaagttgacaatcgaaaaccatttgcaatggtgcccggaattcctcaatgttgtcaacctgactcttggtcaatggtgcctggattcaaacttctatgccctaattgtcttccttcagaactcacctagactacagaagctaactctgaaactcgaaaaggtaaatgctttcgtccaaatgtgcactgatcctacttgattgctttttacacaagagttttcacatcgggatatagatcacctgtttttgttgctgtagagtaatatgagaataagtcgaagaatcattggcgagcttacggaaatatcgtttacatgcgagcaccttaatactgttgaagtgatatgctcggagaatgatccccaggtcatcactgtgcaagatttctttgttagcagtggcatgacctctgttcagtttcatatcaaacactggagcccgtacgcgaatgacctaccggcatttattcggtcgatatgaaatggcaaaggatcacgaattagtttgccatcccattgaagaaaatcactctctttcgcctgtcggctacatctgctggttacagtagagtgttcagtgatcttgtttggtgctttctgctatgacgtgaaggtgagtagaaacaattgcatcttgccttgcatgtaaggtcagtggtattgtacttgtttgctgcgatcacatgatgatttgtagcaataatgatgctccaagaaaaatactgtatggctatggcgtctcctgatacagcctgccactgctttctcaacttggctggtctccggtgaaacgtattgtatttgtctctctcctaatataatcccatgttatagtgttgt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070622.1 RefSeq:Os10g0127900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 10]]<br /> [[Category:Chromosome 10]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os12g0111500&diff=176255 Os12g0111500 2014-06-02T12:00:45Z <p>Jieomlong: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os12g0111500|<br /> Description = BTB domain containing protein|<br /> Version = NM_001072500.1 GI:115486961 GeneID:4351297|<br /> Length = 1837 bp|<br /> Definition = Oryza sativa Japonica Group Os12g0111500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 12|Chromosome 12]]|<br /> AP = Chromosome 12:601666..603502|<br /> CDS = 601796..602257,602353..602529,603210..603398|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008405:601666..603502<br /> source=RiceChromosome12<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008405:601666..603502<br /> source=RiceChromosome12<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggattgctgcgtgtgcagtcccatggcgtccatgtacaggcttccaaggaacgccatctgcgctgcgtgctatgaaggtgccaaggccatcatcgccttcttcaacgacgacgacgacgagcacgccgatgctgatcaaggctcggtgaagcccagcagattgacaaagctcaatagcacaaccaagggattgagagatgcgtgggaggaggtgaagcagatgcgatgcagagaggaggagaccaagcagagggcatcgtttctccagcaaggcttcgcggcggcgtggaaagacggcatccacaccgacatcgccgtcaggcctggaactgggcccccaatccaagcccataaggccatccttgcgaccaggtcggaggtgttccgccacatcctcgccggcgacgacgactgcaaggcccccgccggcgactctctgtcgctaccggagctgacccacgacgagctctcccacctcctcgccttcctctacacgggctccctggcgacgtgcacggaggagcggcacctgcacgcgctgctggtcgccggcgacaagtacgacgtgccgttcctccggcgagcgtgcgaggcgcggctggcggcgggggtggaggcgggcaacgtgctgcggacgctggaggtggcggagctgagctcgagcgcggcgctcaaggagcgcgccatgggggcggtggtggagcacgcggaggaggtggtgttctcgccggagtacgaggagttcgccgtcaggaacgccgccctttgcgtccagatcacccgcgctctgcttgccaataaagcatttcccgccaagacgccttaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MDCCVCSPMASMYRLPRNAICAACYEGAKAIIAFFNDDDDEHAD ADQGSVKPSRLTKLNSTTKGLRDAWEEVKQMRCREEETKQRASFLQQGFAAAWKDGIH TDIAVRPGTGPPIQAHKAILATRSEVFRHILAGDDDCKAPAGDSLSLPELTHDELSHL LAFLYTGSLATCTEERHLHALLVAGDKYDVPFLRRACEARLAAGVEAGNVLRTLEVAE LSSSAALKERAMGAVVEHAEEVVFSPEYEEFAVRNAALCVQITRALLANKAFPAKTP&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1246..1707#974..1150#105..293#aatgctagcgttgctgttcacgtcgttgccaagccacaaggagaaggaagaaggaagagaaggcagaaggaagcagcaggagaaggagaaggccagaagaaacaatggattgctgcgtgtgcagtcccatggcgtccatgtacaggcttccaaggaacgccatctgcgctgcgtgctatgaaggtgccaaggccatcatcgccttcttcaacgacgacgacgacgagcacgccgatgctgatcaaggctcggtgaagcccagcagattgacaaagctcaatagcacaaccaaggtaagaccttcccatatctcattttgctcctttattaattagtcctttaggattggtatgactatgttcggtttacaaccatctcgattgtgccgtgtgctctacttccatgaaaaatctagccttgcgtgtttctagattctagaagcagagaaatttcggtagagaatgaattgataaaaaggaattccatctcacatgtaatgcaatataaactcttgttgcattggttacatcattcagtttcattgacgcatgcgtcaggtttgctgtcaacgttgtgttggttaatggtgaggagcaagtctcaatcgagagttgcatgagaatgataggtttggtggaaatctcattgtcaaaaggttcatgattgatcgatgcttaaagaccgttttcagaagctgtattttatatactagtgtgcttcaacagctgatgttatctttttattaaaaaaccaaaccggcatgacatacacaagattctccttgattgcaatagctggaatgaggaaataaaggtgcatgccttggacaagttgttatgctaatcttcagaaagaattaattctgaacagtcacagattaatgctaattggtgggggtgcatgggacatacatactacacttatcagcatcatagtgctaactgctaacatttgtgcgtgttactcaatgtagggattgagagatgcgtgggaggaggtgaagcagatgcgatgcagagaggaggagaccaagcagagggcatcgtttctccagcaaggcttcgcggcggcgtggaaagacggcatccacaccgacatcgccgtcaggcctggaactgggcccccaatccaagcccataaggccatccttgtcagtctctctctctctcagtttcagcccaataggccggccttcttttagtttgattgggcccaatgaaactaattaggcccagctatatacaggcgaccaggtcggaggtgttccgccacatcctcgccggcgacgacgactgcaaggcccccgccggcgactctctgtcgctaccggagctgacccacgacgagctctcccacctcctcgccttcctctacacgggctccctggcgacgtgcacggaggagcggcacctgcacgcgctgctggtcgccggcgacaagtacgacgtgccgttcctccggcgagcgtgcgaggcgcggctggcggcgggggtggaggcgggcaacgtgctgcggacgctggaggtggcggagctgagctcgagcgcggcgctcaaggagcgcgccatgggggcggtggtggagcacgcggaggaggtggtgttctcgccggagtacgaggagttcgccgtcaggaacgccgccctttgcgtccagatcacccgcgctctgcttgccaataaagcatttcccgccaagacgccttaatatcccttttatttttcttcgtttagaatttagttagacgacgtacgatgtataagtgtaattaaatgatagtacatgcatatctctggaattaatgtgatcaatatatacaatttagattctttgcacg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001072500.1 RefSeq:Os12g0111500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 12]]<br /> [[Category:Chromosome 12]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os12g0111500&diff=176254 Os12g0111500 2014-06-02T12:00:01Z <p>Jieomlong: </p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> F-box proteins comprise one of the large protein families in<br /> plants. There are more than 692 and 779 F-box genes in the<br /> A. thaliana and rice genomes, respectively (Xu et al., 2009).<br /> Previous studies demonstrated that F-box proteins are involved<br /> in the regulation of several biological processes, including circadian<br /> clock, self-incompatibility, photomorphogenesis, floral<br /> meristem, phytohormonal signalling, and responses to abiotic<br /> stresses (Sullivan et al., 2003; Moon et al., 2004; Ni et al.,<br /> 2004; Smalle and Vierstra, 2004; Dreher and Callis, 2007). For<br /> instance, the F-box protein TIR1/AFB can bind to the transcriptional<br /> repressor protein Aux/IAA, thus promoting their proteasomal<br /> degradation and activating auxin-induced transcription<br /> (Dharmasiri et al., 2005; Tan et al., 2007). The involvement of<br /> F-box proteins in response to both biotic and abiotic stresses has<br /> also been reported.<br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os12g0111500|<br /> Description = BTB domain containing protein|<br /> Version = NM_001072500.1 GI:115486961 GeneID:4351297|<br /> Length = 1837 bp|<br /> Definition = Oryza sativa Japonica Group Os12g0111500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 12|Chromosome 12]]|<br /> AP = Chromosome 12:601666..603502|<br /> CDS = 601796..602257,602353..602529,603210..603398|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008405:601666..603502<br /> source=RiceChromosome12<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008405:601666..603502<br /> source=RiceChromosome12<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggattgctgcgtgtgcagtcccatggcgtccatgtacaggcttccaaggaacgccatctgcgctgcgtgctatgaaggtgccaaggccatcatcgccttcttcaacgacgacgacgacgagcacgccgatgctgatcaaggctcggtgaagcccagcagattgacaaagctcaatagcacaaccaagggattgagagatgcgtgggaggaggtgaagcagatgcgatgcagagaggaggagaccaagcagagggcatcgtttctccagcaaggcttcgcggcggcgtggaaagacggcatccacaccgacatcgccgtcaggcctggaactgggcccccaatccaagcccataaggccatccttgcgaccaggtcggaggtgttccgccacatcctcgccggcgacgacgactgcaaggcccccgccggcgactctctgtcgctaccggagctgacccacgacgagctctcccacctcctcgccttcctctacacgggctccctggcgacgtgcacggaggagcggcacctgcacgcgctgctggtcgccggcgacaagtacgacgtgccgttcctccggcgagcgtgcgaggcgcggctggcggcgggggtggaggcgggcaacgtgctgcggacgctggaggtggcggagctgagctcgagcgcggcgctcaaggagcgcgccatgggggcggtggtggagcacgcggaggaggtggtgttctcgccggagtacgaggagttcgccgtcaggaacgccgccctttgcgtccagatcacccgcgctctgcttgccaataaagcatttcccgccaagacgccttaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MDCCVCSPMASMYRLPRNAICAACYEGAKAIIAFFNDDDDEHAD ADQGSVKPSRLTKLNSTTKGLRDAWEEVKQMRCREEETKQRASFLQQGFAAAWKDGIH TDIAVRPGTGPPIQAHKAILATRSEVFRHILAGDDDCKAPAGDSLSLPELTHDELSHL LAFLYTGSLATCTEERHLHALLVAGDKYDVPFLRRACEARLAAGVEAGNVLRTLEVAE LSSSAALKERAMGAVVEHAEEVVFSPEYEEFAVRNAALCVQITRALLANKAFPAKTP&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1246..1707#974..1150#105..293#aatgctagcgttgctgttcacgtcgttgccaagccacaaggagaaggaagaaggaagagaaggcagaaggaagcagcaggagaaggagaaggccagaagaaacaatggattgctgcgtgtgcagtcccatggcgtccatgtacaggcttccaaggaacgccatctgcgctgcgtgctatgaaggtgccaaggccatcatcgccttcttcaacgacgacgacgacgagcacgccgatgctgatcaaggctcggtgaagcccagcagattgacaaagctcaatagcacaaccaaggtaagaccttcccatatctcattttgctcctttattaattagtcctttaggattggtatgactatgttcggtttacaaccatctcgattgtgccgtgtgctctacttccatgaaaaatctagccttgcgtgtttctagattctagaagcagagaaatttcggtagagaatgaattgataaaaaggaattccatctcacatgtaatgcaatataaactcttgttgcattggttacatcattcagtttcattgacgcatgcgtcaggtttgctgtcaacgttgtgttggttaatggtgaggagcaagtctcaatcgagagttgcatgagaatgataggtttggtggaaatctcattgtcaaaaggttcatgattgatcgatgcttaaagaccgttttcagaagctgtattttatatactagtgtgcttcaacagctgatgttatctttttattaaaaaaccaaaccggcatgacatacacaagattctccttgattgcaatagctggaatgaggaaataaaggtgcatgccttggacaagttgttatgctaatcttcagaaagaattaattctgaacagtcacagattaatgctaattggtgggggtgcatgggacatacatactacacttatcagcatcatagtgctaactgctaacatttgtgcgtgttactcaatgtagggattgagagatgcgtgggaggaggtgaagcagatgcgatgcagagaggaggagaccaagcagagggcatcgtttctccagcaaggcttcgcggcggcgtggaaagacggcatccacaccgacatcgccgtcaggcctggaactgggcccccaatccaagcccataaggccatccttgtcagtctctctctctctcagtttcagcccaataggccggccttcttttagtttgattgggcccaatgaaactaattaggcccagctatatacaggcgaccaggtcggaggtgttccgccacatcctcgccggcgacgacgactgcaaggcccccgccggcgactctctgtcgctaccggagctgacccacgacgagctctcccacctcctcgccttcctctacacgggctccctggcgacgtgcacggaggagcggcacctgcacgcgctgctggtcgccggcgacaagtacgacgtgccgttcctccggcgagcgtgcgaggcgcggctggcggcgggggtggaggcgggcaacgtgctgcggacgctggaggtggcggagctgagctcgagcgcggcgctcaaggagcgcgccatgggggcggtggtggagcacgcggaggaggtggtgttctcgccggagtacgaggagttcgccgtcaggaacgccgccctttgcgtccagatcacccgcgctctgcttgccaataaagcatttcccgccaagacgccttaatatcccttttatttttcttcgtttagaatttagttagacgacgtacgatgtataagtgtaattaaatgatagtacatgcatatctctggaattaatgtgatcaatatatacaatttagattctttgcacg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001072500.1 RefSeq:Os12g0111500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 12]]<br /> [[Category:Chromosome 12]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=User:Jieomlong&diff=176252 User:Jieomlong 2014-06-02T11:57:37Z <p>Jieomlong: </p> <hr /> <div>Please input one-sentence summary here.<br /> F-box proteins play diverse roles in regulating numerous physiological processes in plants. This study isolated a gene (OsFbx352) from rice encoding an F-box domain protein and characterized its role in seed germination. Expression of OsFbx352 was upregulated by abscisic acid (ABA). <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> 1.OsFbx352 encodes a F-box domain protein and is suppressed by glucose.<br /> 2.Generation of transgenic rice plants with altered.<br /> 3.Germination of transgenic seeds displayed different sensitivity to ABA expression of OsFbx352.<br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> F-box proteins comprise one of the large protein families in plants. There are more than 692 and 779 F-box genes in the A. thaliana and rice genomes, respectively (Xu et al., 2009).Previous studies demonstrated that F-box proteins are involved in the regulation of several biological processes, including circadian clock, self-incompatibility, photomorphogenesis, floral meristem, phytohormonal signalling, and responses to abiotic stresses (Sullivan et al., 2003; Moon et al., 2004; Ni et al.,2004; Smalle and Vierstra, 2004; Dreher and Callis, 2007). For instance, the F-box protein TIR1/AFB can bind to the transcriptional repressor protein Aux/IAA, thus promoting their proteasomal degradation and activating auxin-induced transcription (Dharmasiri et al., 2005; Tan et al., 2007). The involvement of<br /> F-box proteins in response to both biotic and abiotic stresses has also been reported.<br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{IndicaGene|<br /> GeneName = BGIOSGA000063|<br /> Description = Putative uncharacterized protein|<br /> Definition = Oryza sativa Indica Group BGIOSGA000063, complete gene.|<br /> tag = P0483G10.23|<br /> tid = BGIOSGA000063-TA|<br /> pid = BGIOSGA000063-PA|<br /> Length = 2809|<br /> Source = Oryza sativa Indica Group<br /> <br /> ORGANISM Oryza sativa Indica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Indica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:46486990..46489798|<br /> CDS = 46489660..46489798,46489009..46489108,46488808..46488928,46488319..46488431,46488095..46488216&lt;br&gt;,46487646..46487805,46486990..46487110|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=IndicaChromosome01:46486990..46489798<br /> source=RiceIndica01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=IndicaChromosome01:46486990..46489798<br /> source=RiceIndica01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ATGGATGTGAGCAGGAGGAAGAAGTGGGTGGCGTGGGTGGCGGCGGTCACCATCTTCGTCGTGCTCATGCTCGTCACCCCGGCCATCCCGCAGAACGAGGAGTACCACGACTTCGCCGACCAGCGCCGCCTCTTCCTCGGGATCCCCAATACGCTGAATGTGATTTCAAACATCCCCTTCTTTTTTGTGGGTGTCGTTGGGCTCGTCTTGTGCCATTACAAAAACTACTTCAGGCTAAACTCGCAAGGGGAGCTCTGGAGTTGGACACTGTTCTTTGCTGGTGTGACTGCTGTTGCTTTTGGTTCATCCTATTATCATCTCAAACCGAATGATGCCACACTAGTTTGGGACAGATTGCCGATGACAATTGCTTTCACATCTGTCATGGCCATTTTCATTATTGAGAGGGTTGATGATAGGGCAGGAACAAAGTCCCTAGCACCTCTTGTTATTGCTGGTGCACTAAGCATCATGTTCTTTGACGATCTTCGCCCATATGCAGTAATTCAGTTTGTTCCATGCATTGCAATACCTGTGATGGCTATTGTAATTCCACCAATGTATACACATTCGAGCTATTGGCTGTGGGCAGCAGGATTTTACCTCCTTGCTAAGGTAGAAGAGGCCGCAGATAAACCTATATATAAATGGACCCATCAAATTGTTAGCGGTCATACTCTTAAGCATCTAGCTGCTGCAATGGTGCCTGTGTTCTTGGCACTCATGCTGGCCAAAAGGACTGTTGAACCAGAAAGGGTAAGTTTATTCCAGCGGTGGAAGGTCAAGTTGGTTGCAGTGAGAGAGACTCGATTCAACAAGGACAGGAATACAGTCGACTACAACTATATTGCAGTTTCCACTACACCTGAACCATAG&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MDVSRRKKWVAWVAAVTIFVVLMLVTPAIPQNEEYHDFADQRRLFLGIPNTLNVISNIPFFFVGVVGLVLCHYKNYFRLNSQGELWSWTLFFAGVTAVAFGSSYYHLKPNDATLVWDRLPMTIAFTSVMAIFIIERVDDRAGTKSLAPLVIAGALSIMFFDDLRPYAVIQFVPCIAIPVMAIVIPPMYTHSSYWLWAAGFYLLAKVEEAADKPIYKWTHQIVSGHTLKHLAAAMVPVFLALMLAKRTVEPERVSLFQRWKVKLVAVRETRFNKDRNTVDYNYIAVSTTPEP&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1..139#691..790#871..991#1368..1480#1583..1704#1994..2153#2689..2809#ATGGATGTGAGCAGGAGGAAGAAGTGGGTGGCGTGGGTGGCGGCGGTCACCATCTTCGTCGTGCTCATGCTCGTCACCCCGGCCATCCCGCAGAACGAGGAGTACCACGACTTCGCCGACCAGCGCCGCCTCTTCCTCGGTATGCCACAAACCAACCCTCCCTCCCCGCCGCAAACCTAGGGCGGACCTTCCCTTGCGGCCGCCATAGCTCGCCGTCCGCGGTATTGAGCTCCCGTCTCTCCGAGCTATTGAGCTCTCGGGGTAGCCTCCACCCGGCGCCGGCAAGACGATCCGCGAGCAGGAGAGCAGCGATTGTGCCAGTGCTTGTCCCTGACGGCGCAATGGCCAACGTTGAGTGACGAGTTGACTCGAAATCGCGAATTCGAGGTCGCTTGCATCGCCTAAATCGATCCCCTTTGTATTCACTACAAAAATTGATTGCCCTTTGTTTGCGCTGGGTTCAGCAAGATTAGCTTGTTAGGAAGGATTTTGCAGGCTATACCAGTTTCCTGTTTATTTCACGAACCCAAAATAAACATGAATATAGTATAATTGAGGGGGAATTTGAGGCGCAAACGAATAGAGTTTAAATTTTGCTTATATGTTCAATAAGAGGGTTGCAATGCTTGTAATCACTACTTGATGCACTAAGTTTGCAAAATCATGTGGATTTTTTTTTTTGTTTTGCAGGGATCCCCAATACGCTGAATGTGATTTCAAACATCCCCTTCTTTTTTGTGGGTGTCGTTGGGCTCGTCTTGTGCCATTACAAAAACTACTTCAGGCTAAAGTTAGACCATTACTGGTTCATCTTCTAATTCTAGTTAAGATTTTGATGCACTCTGTTGTTAACCTTCCCCTTCCTGGCAGCTCGCAAGGGGAGCTCTGGAGTTGGACACTGTTCTTTGCTGGTGTGACTGCTGTTGCTTTTGGTTCATCCTATTATCATCTCAAACCGAATGATGCCACACTAGTTTGGGACAGATTGCCGGTGAGTTGTTTCTAGACTTACATTGCCTTGCTCTAGGATTTGTACACACCCCATTATGTTGAAGCTGTCTCAGAATGTCAATGCATACATTTTTTTTGGTGGGATAGAATTCAACTCAGATCCTTGTGTAGTATAATCTGAATATGCTATGGCCCTGTTTAGATAGTGTTGTTCTGTTGGGTGTGATCTGCTTGAATCAAAACAGGGTAACTAATCCACTTGGATCTTTTGCCTGATACGCTCTAGTATATTTGGCTTGTTTTCTTCAGAATTGCCTGTCAGAGACATAAAGCATGTCATCAGTTCATAATTTTCACCCTTGCTTTTATCAATATTTAATATGTTAAAGGATGGCATATTTGCTTGTTCTATGTAGATGACAATTGCTTTCACATCTGTCATGGCCATTTTCATTATTGAGAGGGTTGATGATAGGGCAGGAACAAAGTCCCTAGCACCTCTTGTTATTGCTGGTGCACTAAGCATCATGTATTGGAGGCAAGCTTCTCCAATTGTTTAACCATCCTTCCTCAGAAATATTTTCTGTACTTCTTTCACACCTTCCATTTTGCTGCCTTAACTCTTATGTAGGTTCTTTGACGATCTTCGCCCATATGCAGTAATTCAGTTTGTTCCATGCATTGCAATACCTGTGATGGCTATTGTAATTCCACCAATGTATACACATTCGAGCTATTGGCTGTGGGCAGCAGGTTAGTGTTATTCTTGATCAATAGTATTTGATCTATGTACCTTCATTAGCATTTAGGTTGAAAAATGTGGTATTTGTGGTTCACCTATATATTATGAATCATTCAATTGATCATTTCTCTGCACCTAATAACTGTTGATGATACTTAAATGGTTTTCATTTTAATCTATTTCTACATATCATAAATTGATTCGCAGTGACCAAGTTAATTGATTGGTTTTCTGGAAATTCATACACAATCAGCACCTCACCATATTTTTCTGTTTTCTGATTTCTCAAAACAATTCCAGGATTTTACCTCCTTGCTAAGGTAGAAGAGGCCGCAGATAAACCTATATATAAATGGACCCATCAAATTGTTAGCGGTCATACTCTTAAGCATCTAGCTGCTGCAATGGTGCCTGTGTTCTTGGCACTCATGCTGGCCAAAAGGACTGTTGAACCAGAAAGGTATGCTTTATATACTGGCGTTTTAAGAACCACGTCTTTACTTCGATAAGTGCATCATTGGGTTCAACTCTTGAACTGTTCTTCTATTTAAGCTGTTGAATGGACCTTGGTCAGAATTCAGAAGATATACTTGCGTTGGCATTGTTACTTTGTCTTAAGGAATTATTCCATCATAAATTATTGAGCTATCTGTTCTATTAATTTTGCAAATGCAATCACTATTTGTTTTTGGGAAAAGAATGAAGTTTGTTTTCCTTCGGTTAGAATAAATTCATAAATGCCGTTGTAACTTTTGAATTAGTGTGTTGTTTGTTTCGCCAGTAATTCCATGGTGGTTCTCCCATAGTCCCATATCTTTGCTTCTTAGGATGAGCATATTTCAAATAATTTTATCCCCTCCATTGCAAACGATATCTGTTGTCTGTTGATTGATTTGTCAGCCTTTTTTTTAGCTCTGTTAATATCATGATTAAATTTATGAAGTCTTGCATAACTCTACCCCGTGTCTCATGATATTTTTTTTTCTGGAATGCAGGGTAAGTTTATTCCAGCGGTGGAAGGTCAAGTTGGTTGCAGTGAGAGAGACTCGATTCAACAAGGACAGGAATACAGTCGACTACAACTATATTGCAGTTTCCACTACACCTGAACCATAG&lt;/dnaseqindica&gt;|<br /> Link = [http://plants.ensembl.org/Oryza_indica/Gene/Summary?g=BGIOSGA000063 EnsemblPlants:BGIOSGA000063]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Indica mRNA]]<br /> [[Category:Oryza Sativa Indica Group]]<br /> [[Category:Indica Genes]]<br /> [[Category:Indica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div> Jieomlong https://ngdc.cncb.ac.cn/ricewiki/index.php?title=User:Jieomlong&diff=176236 User:Jieomlong 2014-06-02T11:43:50Z <p>Jieomlong: Created page with &quot;Please input one-sentence summary here. ==Annotated Information== ===Function=== Please input function information here. ===Expression=== Please input expression information...&quot;</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{IndicaGene|<br /> GeneName = BGIOSGA000063|<br /> Description = Putative uncharacterized protein|<br /> Definition = Oryza sativa Indica Group BGIOSGA000063, complete gene.|<br /> tag = P0483G10.23|<br /> tid = BGIOSGA000063-TA|<br /> pid = BGIOSGA000063-PA|<br /> Length = 2809|<br /> Source = Oryza sativa Indica Group<br /> <br /> ORGANISM Oryza sativa Indica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Indica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:46486990..46489798|<br /> CDS = 46489660..46489798,46489009..46489108,46488808..46488928,46488319..46488431,46488095..46488216&lt;br&gt;,46487646..46487805,46486990..46487110|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=IndicaChromosome01:46486990..46489798<br /> source=RiceIndica01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=IndicaChromosome01:46486990..46489798<br /> source=RiceIndica01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;ATGGATGTGAGCAGGAGGAAGAAGTGGGTGGCGTGGGTGGCGGCGGTCACCATCTTCGTCGTGCTCATGCTCGTCACCCCGGCCATCCCGCAGAACGAGGAGTACCACGACTTCGCCGACCAGCGCCGCCTCTTCCTCGGGATCCCCAATACGCTGAATGTGATTTCAAACATCCCCTTCTTTTTTGTGGGTGTCGTTGGGCTCGTCTTGTGCCATTACAAAAACTACTTCAGGCTAAACTCGCAAGGGGAGCTCTGGAGTTGGACACTGTTCTTTGCTGGTGTGACTGCTGTTGCTTTTGGTTCATCCTATTATCATCTCAAACCGAATGATGCCACACTAGTTTGGGACAGATTGCCGATGACAATTGCTTTCACATCTGTCATGGCCATTTTCATTATTGAGAGGGTTGATGATAGGGCAGGAACAAAGTCCCTAGCACCTCTTGTTATTGCTGGTGCACTAAGCATCATGTTCTTTGACGATCTTCGCCCATATGCAGTAATTCAGTTTGTTCCATGCATTGCAATACCTGTGATGGCTATTGTAATTCCACCAATGTATACACATTCGAGCTATTGGCTGTGGGCAGCAGGATTTTACCTCCTTGCTAAGGTAGAAGAGGCCGCAGATAAACCTATATATAAATGGACCCATCAAATTGTTAGCGGTCATACTCTTAAGCATCTAGCTGCTGCAATGGTGCCTGTGTTCTTGGCACTCATGCTGGCCAAAAGGACTGTTGAACCAGAAAGGGTAAGTTTATTCCAGCGGTGGAAGGTCAAGTTGGTTGCAGTGAGAGAGACTCGATTCAACAAGGACAGGAATACAGTCGACTACAACTATATTGCAGTTTCCACTACACCTGAACCATAG&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MDVSRRKKWVAWVAAVTIFVVLMLVTPAIPQNEEYHDFADQRRLFLGIPNTLNVISNIPFFFVGVVGLVLCHYKNYFRLNSQGELWSWTLFFAGVTAVAFGSSYYHLKPNDATLVWDRLPMTIAFTSVMAIFIIERVDDRAGTKSLAPLVIAGALSIMFFDDLRPYAVIQFVPCIAIPVMAIVIPPMYTHSSYWLWAAGFYLLAKVEEAADKPIYKWTHQIVSGHTLKHLAAAMVPVFLALMLAKRTVEPERVSLFQRWKVKLVAVRETRFNKDRNTVDYNYIAVSTTPEP&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1..139#691..790#871..991#1368..1480#1583..1704#1994..2153#2689..2809#ATGGATGTGAGCAGGAGGAAGAAGTGGGTGGCGTGGGTGGCGGCGGTCACCATCTTCGTCGTGCTCATGCTCGTCACCCCGGCCATCCCGCAGAACGAGGAGTACCACGACTTCGCCGACCAGCGCCGCCTCTTCCTCGGTATGCCACAAACCAACCCTCCCTCCCCGCCGCAAACCTAGGGCGGACCTTCCCTTGCGGCCGCCATAGCTCGCCGTCCGCGGTATTGAGCTCCCGTCTCTCCGAGCTATTGAGCTCTCGGGGTAGCCTCCACCCGGCGCCGGCAAGACGATCCGCGAGCAGGAGAGCAGCGATTGTGCCAGTGCTTGTCCCTGACGGCGCAATGGCCAACGTTGAGTGACGAGTTGACTCGAAATCGCGAATTCGAGGTCGCTTGCATCGCCTAAATCGATCCCCTTTGTATTCACTACAAAAATTGATTGCCCTTTGTTTGCGCTGGGTTCAGCAAGATTAGCTTGTTAGGAAGGATTTTGCAGGCTATACCAGTTTCCTGTTTATTTCACGAACCCAAAATAAACATGAATATAGTATAATTGAGGGGGAATTTGAGGCGCAAACGAATAGAGTTTAAATTTTGCTTATATGTTCAATAAGAGGGTTGCAATGCTTGTAATCACTACTTGATGCACTAAGTTTGCAAAATCATGTGGATTTTTTTTTTTGTTTTGCAGGGATCCCCAATACGCTGAATGTGATTTCAAACATCCCCTTCTTTTTTGTGGGTGTCGTTGGGCTCGTCTTGTGCCATTACAAAAACTACTTCAGGCTAAAGTTAGACCATTACTGGTTCATCTTCTAATTCTAGTTAAGATTTTGATGCACTCTGTTGTTAACCTTCCCCTTCCTGGCAGCTCGCAAGGGGAGCTCTGGAGTTGGACACTGTTCTTTGCTGGTGTGACTGCTGTTGCTTTTGGTTCATCCTATTATCATCTCAAACCGAATGATGCCACACTAGTTTGGGACAGATTGCCGGTGAGTTGTTTCTAGACTTACATTGCCTTGCTCTAGGATTTGTACACACCCCATTATGTTGAAGCTGTCTCAGAATGTCAATGCATACATTTTTTTTGGTGGGATAGAATTCAACTCAGATCCTTGTGTAGTATAATCTGAATATGCTATGGCCCTGTTTAGATAGTGTTGTTCTGTTGGGTGTGATCTGCTTGAATCAAAACAGGGTAACTAATCCACTTGGATCTTTTGCCTGATACGCTCTAGTATATTTGGCTTGTTTTCTTCAGAATTGCCTGTCAGAGACATAAAGCATGTCATCAGTTCATAATTTTCACCCTTGCTTTTATCAATATTTAATATGTTAAAGGATGGCATATTTGCTTGTTCTATGTAGATGACAATTGCTTTCACATCTGTCATGGCCATTTTCATTATTGAGAGGGTTGATGATAGGGCAGGAACAAAGTCCCTAGCACCTCTTGTTATTGCTGGTGCACTAAGCATCATGTATTGGAGGCAAGCTTCTCCAATTGTTTAACCATCCTTCCTCAGAAATATTTTCTGTACTTCTTTCACACCTTCCATTTTGCTGCCTTAACTCTTATGTAGGTTCTTTGACGATCTTCGCCCATATGCAGTAATTCAGTTTGTTCCATGCATTGCAATACCTGTGATGGCTATTGTAATTCCACCAATGTATACACATTCGAGCTATTGGCTGTGGGCAGCAGGTTAGTGTTATTCTTGATCAATAGTATTTGATCTATGTACCTTCATTAGCATTTAGGTTGAAAAATGTGGTATTTGTGGTTCACCTATATATTATGAATCATTCAATTGATCATTTCTCTGCACCTAATAACTGTTGATGATACTTAAATGGTTTTCATTTTAATCTATTTCTACATATCATAAATTGATTCGCAGTGACCAAGTTAATTGATTGGTTTTCTGGAAATTCATACACAATCAGCACCTCACCATATTTTTCTGTTTTCTGATTTCTCAAAACAATTCCAGGATTTTACCTCCTTGCTAAGGTAGAAGAGGCCGCAGATAAACCTATATATAAATGGACCCATCAAATTGTTAGCGGTCATACTCTTAAGCATCTAGCTGCTGCAATGGTGCCTGTGTTCTTGGCACTCATGCTGGCCAAAAGGACTGTTGAACCAGAAAGGTATGCTTTATATACTGGCGTTTTAAGAACCACGTCTTTACTTCGATAAGTGCATCATTGGGTTCAACTCTTGAACTGTTCTTCTATTTAAGCTGTTGAATGGACCTTGGTCAGAATTCAGAAGATATACTTGCGTTGGCATTGTTACTTTGTCTTAAGGAATTATTCCATCATAAATTATTGAGCTATCTGTTCTATTAATTTTGCAAATGCAATCACTATTTGTTTTTGGGAAAAGAATGAAGTTTGTTTTCCTTCGGTTAGAATAAATTCATAAATGCCGTTGTAACTTTTGAATTAGTGTGTTGTTTGTTTCGCCAGTAATTCCATGGTGGTTCTCCCATAGTCCCATATCTTTGCTTCTTAGGATGAGCATATTTCAAATAATTTTATCCCCTCCATTGCAAACGATATCTGTTGTCTGTTGATTGATTTGTCAGCCTTTTTTTTAGCTCTGTTAATATCATGATTAAATTTATGAAGTCTTGCATAACTCTACCCCGTGTCTCATGATATTTTTTTTTCTGGAATGCAGGGTAAGTTTATTCCAGCGGTGGAAGGTCAAGTTGGTTGCAGTGAGAGAGACTCGATTCAACAAGGACAGGAATACAGTCGACTACAACTATATTGCAGTTTCCACTACACCTGAACCATAG&lt;/dnaseqindica&gt;|<br /> Link = [http://plants.ensembl.org/Oryza_indica/Gene/Summary?g=BGIOSGA000063 EnsemblPlants:BGIOSGA000063]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Indica mRNA]]<br /> [[Category:Oryza Sativa Indica Group]]<br /> [[Category:Indica Genes]]<br /> [[Category:Indica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div> Jieomlong