http://192.168.164.12:81/ricewiki/api.php?action=feedcontributions&feedformat=atom&user=Jirongli RiceWiki - User contributions [en] 2026-08-27T15:16:40Z User contributions MediaWiki 1.30.0 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=181048 AB013448 2014-06-08T09:24:32Z <p>Jirongli: /* Expression */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.【5】<br /> Link = [http://www.ricedata.cn/reference/list/1419.htm]|<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> Link = [http://www.ricedata.cn/gene/list/70.htm]|<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.【3】<br /> Link = [http://www.ricedata.cn/reference/list/507.htm]|<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.【1】Link = [http://www.ricedata.cn/reference/list/13405.htm]|<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> <br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA =&lt;dnaseqindica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421#3481..3541#3601..3661#3721..3781#3841..3901#3961..4021#4081..4141#4201..4261#<br /> 4321..4381#4441..4501#4561..4621#4681..4741#4801..4861#4921..4981#5041..5101#5161..5221#5281..5341#5401..5461#5521..5581#5641..5701#5761..5821#5881..5941#6001..6061#6121..6181#<br /> 6241..6301#6361..6421#6481..6541#6601..6661#6721..6781#6841..6901#6961..7021#7081..7141#7201..7261#7321..7381#7441..7501#7561..7621#7681..7741#7801..7861 #7921..7981#8041..8101#8161..8221#8281..8341#8401..8461#8521..8581# 8641..8701#8761..8821#8881..8941#9001..9061#9121..9181#9241..9301#9361..9421#9481..9541#9601..9661#9721..9781#9841..9901#9961..10021#10081..10141#10201..10261#10321# cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt <br /> tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg <br /> agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc cg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=181046 AB013448 2014-06-08T09:23:09Z <p>Jirongli: /* Evolution */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.【5】<br /> Link = [http://www.ricedata.cn/reference/list/1419.htm]|<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> Link = [http://www.ricedata.cn/gene/list/70.htm]|<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.【2】<br /> Link = [http://www.ricedata.cn/reference/list/1419.htm]|<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.【1】Link = [http://www.ricedata.cn/reference/list/13405.htm]|<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> <br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA =&lt;dnaseqindica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421#3481..3541#3601..3661#3721..3781#3841..3901#3961..4021#4081..4141#4201..4261#<br /> 4321..4381#4441..4501#4561..4621#4681..4741#4801..4861#4921..4981#5041..5101#5161..5221#5281..5341#5401..5461#5521..5581#5641..5701#5761..5821#5881..5941#6001..6061#6121..6181#<br /> 6241..6301#6361..6421#6481..6541#6601..6661#6721..6781#6841..6901#6961..7021#7081..7141#7201..7261#7321..7381#7441..7501#7561..7621#7681..7741#7801..7861 #7921..7981#8041..8101#8161..8221#8281..8341#8401..8461#8521..8581# 8641..8701#8761..8821#8881..8941#9001..9061#9121..9181#9241..9301#9361..9421#9481..9541#9601..9661#9721..9781#9841..9901#9961..10021#10081..10141#10201..10261#10321# cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt <br /> tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg <br /> agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc cg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=181044 AB013448 2014-06-08T09:22:05Z <p>Jirongli: /* Expression */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.【5】<br /> Link = [http://www.ricedata.cn/reference/list/1419.htm]|<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> Link = [http://www.ricedata.cn/gene/list/70.htm]|<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.【2】<br /> Link = [http://www.ricedata.cn/reference/list/1419.htm]|<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.【1】<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> <br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA =&lt;dnaseqindica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421#3481..3541#3601..3661#3721..3781#3841..3901#3961..4021#4081..4141#4201..4261#<br /> 4321..4381#4441..4501#4561..4621#4681..4741#4801..4861#4921..4981#5041..5101#5161..5221#5281..5341#5401..5461#5521..5581#5641..5701#5761..5821#5881..5941#6001..6061#6121..6181#<br /> 6241..6301#6361..6421#6481..6541#6601..6661#6721..6781#6841..6901#6961..7021#7081..7141#7201..7261#7321..7381#7441..7501#7561..7621#7681..7741#7801..7861 #7921..7981#8041..8101#8161..8221#8281..8341#8401..8461#8521..8581# 8641..8701#8761..8821#8881..8941#9001..9061#9121..9181#9241..9301#9361..9421#9481..9541#9601..9661#9721..9781#9841..9901#9961..10021#10081..10141#10201..10261#10321# cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt <br /> tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg <br /> agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc cg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=181038 AB013448 2014-06-08T09:19:57Z <p>Jirongli: /* Function */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.【5】<br /> Link = [http://www.ricedata.cn/reference/list/1419.htm]|<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> Link = [http://www.ricedata.cn/gene/list/70.htm]|<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.【2】<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.【1】<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> <br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA =&lt;dnaseqindica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421#3481..3541#3601..3661#3721..3781#3841..3901#3961..4021#4081..4141#4201..4261#<br /> 4321..4381#4441..4501#4561..4621#4681..4741#4801..4861#4921..4981#5041..5101#5161..5221#5281..5341#5401..5461#5521..5581#5641..5701#5761..5821#5881..5941#6001..6061#6121..6181#<br /> 6241..6301#6361..6421#6481..6541#6601..6661#6721..6781#6841..6901#6961..7021#7081..7141#7201..7261#7321..7381#7441..7501#7561..7621#7681..7741#7801..7861 #7921..7981#8041..8101#8161..8221#8281..8341#8401..8461#8521..8581# 8641..8701#8761..8821#8881..8941#9001..9061#9121..9181#9241..9301#9361..9421#9481..9541#9601..9661#9721..9781#9841..9901#9961..10021#10081..10141#10201..10261#10321# cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt <br /> tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg <br /> agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc cg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=181032 AB013448 2014-06-08T09:16:37Z <p>Jirongli: /* Function */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.【5】<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.【2】<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.【1】<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> <br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA =&lt;dnaseqindica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421#3481..3541#3601..3661#3721..3781#3841..3901#3961..4021#4081..4141#4201..4261#<br /> 4321..4381#4441..4501#4561..4621#4681..4741#4801..4861#4921..4981#5041..5101#5161..5221#5281..5341#5401..5461#5521..5581#5641..5701#5761..5821#5881..5941#6001..6061#6121..6181#<br /> 6241..6301#6361..6421#6481..6541#6601..6661#6721..6781#6841..6901#6961..7021#7081..7141#7201..7261#7321..7381#7441..7501#7561..7621#7681..7741#7801..7861 #7921..7981#8041..8101#8161..8221#8281..8341#8401..8461#8521..8581# 8641..8701#8761..8821#8881..8941#9001..9061#9121..9181#9241..9301#9361..9421#9481..9541#9601..9661#9721..9781#9841..9901#9961..10021#10081..10141#10201..10261#10321# cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt <br /> tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg <br /> agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc cg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=181029 AB013448 2014-06-08T09:15:31Z <p>Jirongli: /* Expression */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.【2】<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.【1】<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> <br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA =&lt;dnaseqindica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421#3481..3541#3601..3661#3721..3781#3841..3901#3961..4021#4081..4141#4201..4261#<br /> 4321..4381#4441..4501#4561..4621#4681..4741#4801..4861#4921..4981#5041..5101#5161..5221#5281..5341#5401..5461#5521..5581#5641..5701#5761..5821#5881..5941#6001..6061#6121..6181#<br /> 6241..6301#6361..6421#6481..6541#6601..6661#6721..6781#6841..6901#6961..7021#7081..7141#7201..7261#7321..7381#7441..7501#7561..7621#7681..7741#7801..7861 #7921..7981#8041..8101#8161..8221#8281..8341#8401..8461#8521..8581# 8641..8701#8761..8821#8881..8941#9001..9061#9121..9181#9241..9301#9361..9421#9481..9541#9601..9661#9721..9781#9841..9901#9961..10021#10081..10141#10201..10261#10321# cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt <br /> tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg <br /> agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc cg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=181027 AB013448 2014-06-08T09:13:57Z <p>Jirongli: /* Evolution */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.【1】<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> <br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA =&lt;dnaseqindica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421#3481..3541#3601..3661#3721..3781#3841..3901#3961..4021#4081..4141#4201..4261#<br /> 4321..4381#4441..4501#4561..4621#4681..4741#4801..4861#4921..4981#5041..5101#5161..5221#5281..5341#5401..5461#5521..5581#5641..5701#5761..5821#5881..5941#6001..6061#6121..6181#<br /> 6241..6301#6361..6421#6481..6541#6601..6661#6721..6781#6841..6901#6961..7021#7081..7141#7201..7261#7321..7381#7441..7501#7561..7621#7681..7741#7801..7861 #7921..7981#8041..8101#8161..8221#8281..8341#8401..8461#8521..8581# 8641..8701#8761..8821#8881..8941#9001..9061#9121..9181#9241..9301#9361..9421#9481..9541#9601..9661#9721..9781#9841..9901#9961..10021#10081..10141#10201..10261#10321# cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt <br /> tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg <br /> agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc cg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180573 AB013448 2014-06-08T02:58:41Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> <br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA =&lt;dnaseqindica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421#3481..3541#3601..3661#3721..3781#3841..3901#3961..4021#4081..4141#4201..4261#<br /> 4321..4381#4441..4501#4561..4621#4681..4741#4801..4861#4921..4981#5041..5101#5161..5221#5281..5341#5401..5461#5521..5581#5641..5701#5761..5821#5881..5941#6001..6061#6121..6181#<br /> 6241..6301#6361..6421#6481..6541#6601..6661#6721..6781#6841..6901#6961..7021#7081..7141#7201..7261#7321..7381#7441..7501#7561..7621#7681..7741#7801..7861 #7921..7981#8041..8101#8161..8221#8281..8341#8401..8461#8521..8581# 8641..8701#8761..8821#8881..8941#9001..9061#9121..9181#9241..9301#9361..9421#9481..9541#9601..9661#9721..9781#9841..9901#9961..10021#10081..10141#10201..10261#10321# cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt <br /> tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg <br /> agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc cg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180567 AB013448 2014-06-08T02:57:33Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;<br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA =&lt;dnaseqindica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421#3481..3541#3601..3661#3721..3781#3841..3901#3961..4021#4081..4141#4201..4261#<br /> 4321..4381#4441..4501#4561..4621#4681..4741#4801..4861#4921..4981#5041..5101#5161..5221#5281..5341#5401..5461#5521..5581#5641..5701#5761..5821#5881..5941#6001..6061#6121..6181#<br /> 6241..6301#6361..6421#6481..6541#6601..6661#6721..6781#6841..6901#6961..7021#7081..7141#7201..7261#7321..7381#7441..7501#7561..7621#7681..7741#7801..7861 #7921..7981#8041..8101#8161..8221#8281..8341#8401..8461#8521..8581# 8641..8701#8761..8821#8881..8941#9001..9061#9121..9181#9241..9301#9361..9421#9481..9541#9601..9661#9721..9781#9841..9901#9961..10021#10081..10141#10201..10261#10321# cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt <br /> tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg <br /> agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc cg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180518 AB013448 2014-06-08T02:39:11Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA =&lt;dnaseqindica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421#3481..3541#3601..3661#3721..3781#3841..3901#3961..4021#4081..4141#4201..4261#<br /> 4321..4381#4441..4501#4561..4621#4681..4741#4801..4861#4921..4981#5041..5101#5161..5221#5281..5341#5401..5461#5521..5581#5641..5701#5761..5821#5881..5941#6001..6061#6121..6181#<br /> 6241..6301#6361..6421#6481..6541#6601..6661#6721..6781#6841..6901#6961..7021#7081..7141#7201..7261#7321..7381#7441..7501#7561..7621#7681..7741#7801..7861 #7921..7981#8041..8101#8161..8221#8281..8341#8401..8461#8521..8581# 8641..8701#8761..8821#8881..8941#9001..9061#9121..9181#9241..9301#9361..9421#9481..9541#9601..9661#9721..9781#9841..9901#9961..10021#10081..10141#10201..10261#10321# cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt <br /> tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg <br /> agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc cg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=180516 Os09g0327600 2014-06-08T02:38:54Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot; /&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> The rice blast resistance gene was identified by analyzing 281 recombinant inbred lines (RILs) of an F7 recombinant inbred population of the Moroberekan/CO39 cross with 127 restriction fragment length polymorphism (RFLP) markers&lt;ref name=&quot;ref2&quot; /&gt;.<br /> ===Expression===<br /> ===Evolution===<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to ''Magnaporthe oryzae'' requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Wang G L, Mackill D J, Bonman J M, et al. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar[J]. Genetics, 1994, 136(4): 1421-1434.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = ''Magnaporthe grisea resistance-5''(pi5-1)|<br /> Description = rice blast resistance gene|<br /> Version = GI:213958601 GeneID:4346749|<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> AP = Chromosome 9:9,619,375..9,621,822|<br /> CDS = Chromosome 9:5278..5592,6568..7716,7915..9231,9672..9831,<br /> 10693..10829|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atg <br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga ag<br /> ggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcag<br /> aatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga g<br /> ccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca g<br /> ctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatga<br /> <br /> <br /> <br /> <br /> &lt;/cdnaseq&gt;|<br /> <br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=180513 Os09g0327600 2014-06-08T02:37:59Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot; /&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> The rice blast resistance gene was identified by analyzing 281 recombinant inbred lines (RILs) of an F7 recombinant inbred population of the Moroberekan/CO39 cross with 127 restriction fragment length polymorphism (RFLP) markers&lt;ref name=&quot;ref2&quot; /&gt;.<br /> ===Expression===<br /> ===Evolution===<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to ''Magnaporthe oryzae'' requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Wang G L, Mackill D J, Bonman J M, et al. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar[J]. Genetics, 1994, 136(4): 1421-1434.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = ''Magnaporthe grisea resistance-5''(pi5-1)|<br /> Description = rice blast resistance gene|<br /> Version = GI:213958601 GeneID:4346749|<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> AP = Chromosome 9:9,619,375..9,621,822|<br /> CDS = Chromosome 9:5278..5592,6568..7716,7915..9231,9672..9831,<br /> 10693..10829|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atg <br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga ag<br /> ggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcag<br /> aatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga g<br /> ccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca g<br /> ctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatga<br /> <br /> <br /> <br /> <br /> &lt;/cdnaseq&gt;|<br /> <br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180453 AB013448 2014-06-08T02:11:38Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA =&lt;dnaseqindica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421#3481..3541#3601..3661#3721..3781#3841..3901#3961..4021#4081..4141#4201..4261#<br /> 4321..4381#4441..4501#4561..4621#4681..4741#4801..4861#4921..4981#5041..5101#5161..5221#5281..5341#5401..5461#5521..5581#5641..5701#5761..5821#5881..5941#6001..6061#6121..6181#<br /> 6241..6301#6361..6421#6481..6541#6601..6661#6721..6781#6841..6901#6961..7021#7081..7141#7201..7261#7321..7381#7441..7501#7561..7621#7681..7741#7801..7861 #7921..7981#8041..8101#8161..8221#8281..8341#8401..8461#8521..8581# 8641..8701#8761..8821#8881..8941#9001..9061#9121..9181#9241..9301#9361..9421#9481..9541#9601..9661#9721..9781#9841..9901#9961..10021#10081..10141#10201..10261#10321# cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt <br /> tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg <br /> agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc cg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180442 AB013448 2014-06-08T02:07:52Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqJaponica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421#3481..3541#3601..3661#3721..3781#3841..3901#3961..4021#4081..4141#4201..4261#<br /> 4321..4381#4441..4501#4561..4621#4681..4741#4801..4861#4921..4981#5041..5101#5161..5221#5281..5341#5401..5461#5521..5581#5641..5701#5761..5821#5881..5941#6001..6061#6121..6181#<br /> 6241..6301#6361..6421#6481..6541#6601..6661#6721..6781#6841..6901#6961..7021#7081..7141#7201..7261#7321..7381#7441..7501#7561..7621#7681..7741#7801..7861 #7921..7981#8041..8101#8161..8221#8281..8341#8401..8461#8521..8581# 8641..8701#8761..8821#8881..8941#9001..9061#9121..9181#9241..9301#9361..9421#9481..9541#9601..9661#9721..9781#9841..9901#9961..10021#10081..10141#10201..10261#10321# cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt <br /> tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg <br /> agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc cg&lt;/dnaseqJaponica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180440 AB013448 2014-06-08T02:05:52Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqJaponica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421#3481..3541#3601..3661#3721..3781#3841..3901#3961..4021#4081..4141#4201..4261#<br /> 4321..4381#4441..4501#4561..4621#4681..4741#4801..4861#4921..4981#5041..5101#5161..5221#5281..5341#5401..5461#5521..5581#5641..5701#5761..5821#5881..5941#6001..6061#6121..6181#<br /> 6241..6301#6361..6421#6481..6541#6601..6661#6721..6781#6841..6901#6961..7021#7081..7141#7201..7261#7321..7381#7441..7501#7561..7621#7681..7741#7801..7861 #7921..7981#8041..8101#8161..8221#8281..8341#8401..8461#8521..8581# 8641..8701#8761..8821#8881..8941# 9001..9061#9121..9181#9241..9301#9361..9421#9481..9541#9601..9661#9721..9781#9841..9901#9961..10021#10081..10141#10201..10261#10321# <br /> cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt <br /> tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg <br /> agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag<br /> <br /> gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca<br /> agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat<br /> aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt<br /> aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga<br /> aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc<br /> agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc<br /> cg&lt;/dnaseqJaponica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180415 AB013448 2014-06-08T01:53:59Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqJaponica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421#3481..3541#3601..3661#3721..3781#3841..3901#3961..4021#4081..4141#4201..4261#<br /> 4321..4381#4441..4501#4561..4621#4681..4741#4801..4861#4921..4981#5041..5101#5161..5221#5281..5341#5401..5461#5521..5581#5641..5701#5761..5821#5881..5941#6001..6061#6121..6181#<br /> 6241..6301#6361..6421#6481..6541#6601..6661#6721..6781#6841..6901#6961..7021#7081..7141#7201..7261#7321..7381#7441..7501#7561..7621#7681..7741#7801..7861 #7921..7981#8041..8101#8161..8221#8281..8341#8401..8461#8521..8581# 8641..8701#8761..8821# <br /> cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt <br /> tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg <br /> agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> <br /> tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta<br /> gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga<br /> ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg<br /> ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag<br /> ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta<br /> atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc<br /> gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc<br /> tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca<br /> agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga<br /> aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc<br /> atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa<br /> tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc<br /> tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa<br /> tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag<br /> cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca<br /> ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga<br /> acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt<br /> tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg<br /> gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag<br /> 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#<br /> 8881 gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag<br /> 8941 cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt<br /> 9001 gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga<br /> 9061 cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc<br /> 9121 tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact<br /> 9181 ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta<br /> 9241 agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg<br /> 9301 taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca<br /> 9361 agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc<br /> 9421 gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca<br /> 9481 aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca<br /> 9541 agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc<br /> 9601 acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc<br /> 9661 ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg<br /> 9721 ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg<br /> 9781 gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat<br /> 9841 aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc<br /> 9901 tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag<br /> 9961 ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt<br /> 10021 atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag<br /> 10081 agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt<br /> 10141 aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga<br /> 10201 aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc<br /> 10261 agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc<br /> 10321 cg&lt;/dnaseqJaponica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180406 AB013448 2014-06-08T01:36:13Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqJaponica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421#3481..3541#3601..3661#3721..3781#3841..3901#3961..4021#4081..4141#4201..4261#<br /> 4321..4381#4441..4501#4561..4621#4681..4741#4801..4861#4921..4981#5041..5101#5161..5221#5281..5341#5401..5461#5521..5581#5641..5701#5761..5821#5881..5941#6001..6061#6121..6181#<br /> 6241..6301#6361..6421#6481..6541#6601..6661#6721..6781#6841..6901#6961..7021#7081..7141#7201..7261#7321..7381#7441..7501#7561..7621# <br /> cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt <br /> tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg <br /> agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> <br /> cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat<br /> ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag<br /> ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> 7681 tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta<br /> 7741 gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga<br /> 7801 ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg<br /> 7861 ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag<br /> 7921 ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta<br /> 7981 atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc<br /> 8041 gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc<br /> 8101 tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca<br /> 8161 agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga<br /> 8221 aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc<br /> 8281 atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa<br /> 8341 tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc<br /> 8401 tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa<br /> 8461 tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag<br /> 8521 cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca<br /> 8581 ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> 8641 ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga<br /> 8701 acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt<br /> 8761 tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg<br /> 8821 gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag<br /> 8881 gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag<br /> 8941 cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt<br /> 9001 gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga<br /> 9061 cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc<br /> 9121 tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact<br /> 9181 ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta<br /> 9241 agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg<br /> 9301 taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca<br /> 9361 agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc<br /> 9421 gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca<br /> 9481 aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca<br /> 9541 agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc<br /> 9601 acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc<br /> 9661 ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg<br /> 9721 ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg<br /> 9781 gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat<br /> 9841 aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc<br /> 9901 tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag<br /> 9961 ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt<br /> 10021 atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag<br /> 10081 agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt<br /> 10141 aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga<br /> 10201 aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc<br /> 10261 agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc<br /> 10321 cg&lt;/dnaseqJaponica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180400 AB013448 2014-06-08T01:23:13Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqJaponica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421#3481..3541#3601..3661#3721..3781#3841..3901#3961..4021#4081..4141#4201..4261#<br /> 4321..4381#4441..4501#4561..4621#4681..4741#4801..4861#4921..4981#5041..5101#5161..5221#5281..5341#5401..5461#5521..5581#5641..5701#5761..5821#5881..5941#6001..6061#6121..6181#<br /> cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt <br /> tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg <br /> agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> 6241 cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat<br /> 6301 catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg<br /> 6361 atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc<br /> 6421 gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt<br /> 6481 gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt<br /> 6541 ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa<br /> 6601 ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc<br /> 6661 attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt<br /> 6721 cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata<br /> 6781 ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga<br /> 6841 cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc<br /> 6901 cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc<br /> 6961 acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat<br /> 7021 cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg<br /> 7081 ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag<br /> 7141 tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg<br /> 7201 ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt<br /> 7261 ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt<br /> 7321 tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac<br /> 7381 ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc<br /> 7441 agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca<br /> 7501 aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat<br /> 7561 ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag<br /> 7621 ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> 7681 tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta<br /> 7741 gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga<br /> 7801 ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg<br /> 7861 ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag<br /> 7921 ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta<br /> 7981 atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc<br /> 8041 gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc<br /> 8101 tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca<br /> 8161 agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga<br /> 8221 aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc<br /> 8281 atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa<br /> 8341 tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc<br /> 8401 tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa<br /> 8461 tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag<br /> 8521 cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca<br /> 8581 ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> 8641 ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga<br /> 8701 acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt<br /> 8761 tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg<br /> 8821 gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag<br /> 8881 gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag<br /> 8941 cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt<br /> 9001 gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga<br /> 9061 cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc<br /> 9121 tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact<br /> 9181 ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta<br /> 9241 agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg<br /> 9301 taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca<br /> 9361 agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc<br /> 9421 gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca<br /> 9481 aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca<br /> 9541 agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc<br /> 9601 acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc<br /> 9661 ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg<br /> 9721 ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg<br /> 9781 gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat<br /> 9841 aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc<br /> 9901 tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag<br /> 9961 ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt<br /> 10021 atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag<br /> 10081 agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt<br /> 10141 aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga<br /> 10201 aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc<br /> 10261 agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc<br /> 10321 cg&lt;/dnaseqJaponica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180399 AB013448 2014-06-08T01:16:07Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqJaponica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421#3481..3541#3601..3661#3721..3781#3841..3901#3961..4021#4081..4141#4201..4261#<br /> 4321..4381#4441..4501#4561..4621#4681..4741#4801..4861#4921..4981#5041..5101#5161..5221#5281..5341#<br /> cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt <br /> tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg<br /> 1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341# <br /> agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg<br /> gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa<br /> 5401 aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat<br /> 5461 aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa<br /> 5521 ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat<br /> 5581 ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg<br /> 5641 taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt<br /> 5701 atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt<br /> 5761 tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc<br /> 5821 attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa<br /> 5881 atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat<br /> 5941 agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca<br /> 6001 tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt<br /> 6061 ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact<br /> 6121 tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca<br /> 6181 gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> 6241 cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat<br /> 6301 catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg<br /> 6361 atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc<br /> 6421 gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt<br /> 6481 gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt<br /> 6541 ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa<br /> 6601 ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc<br /> 6661 attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt<br /> 6721 cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata<br /> 6781 ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga<br /> 6841 cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc<br /> 6901 cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc<br /> 6961 acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat<br /> 7021 cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg<br /> 7081 ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag<br /> 7141 tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg<br /> 7201 ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt<br /> 7261 ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt<br /> 7321 tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac<br /> 7381 ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc<br /> 7441 agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca<br /> 7501 aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat<br /> 7561 ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag<br /> 7621 ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> 7681 tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta<br /> 7741 gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga<br /> 7801 ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg<br /> 7861 ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag<br /> 7921 ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta<br /> 7981 atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc<br /> 8041 gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc<br /> 8101 tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca<br /> 8161 agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga<br /> 8221 aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc<br /> 8281 atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa<br /> 8341 tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc<br /> 8401 tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa<br /> 8461 tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag<br /> 8521 cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca<br /> 8581 ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> 8641 ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga<br /> 8701 acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt<br /> 8761 tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg<br /> 8821 gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag<br /> 8881 gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag<br /> 8941 cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt<br /> 9001 gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga<br /> 9061 cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc<br /> 9121 tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact<br /> 9181 ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta<br /> 9241 agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg<br /> 9301 taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca<br /> 9361 agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc<br /> 9421 gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca<br /> 9481 aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca<br /> 9541 agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc<br /> 9601 acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc<br /> 9661 ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg<br /> 9721 ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg<br /> 9781 gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat<br /> 9841 aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc<br /> 9901 tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag<br /> 9961 ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt<br /> 10021 atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag<br /> 10081 agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt<br /> 10141 aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga<br /> 10201 aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc<br /> 10261 agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc<br /> 10321 cg&lt;/dnaseqJaponica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180398 AB013448 2014-06-08T01:07:36Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqJaponica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421#3481..3541#3601..3661#3721..3781#3841..3901#3961..4021#4081..4141#4201..4261#<br /> cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt <br /> tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg<br /> 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341# <br /> 4321 agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga<br /> 4381 agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat<br /> 4441 gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg<br /> 4501 caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc<br /> 4561 agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat<br /> 4621 atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt<br /> 4681 gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc<br /> 4741 tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> 4801 agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg<br /> 4861 tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat<br /> 4921 tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat<br /> 4981 attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca<br /> 5041 aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt<br /> 5101 ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca<br /> 5161 aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga<br /> 5221 gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> 5281 caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg<br /> 5341 gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa<br /> 5401 aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat<br /> 5461 aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa<br /> 5521 ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat<br /> 5581 ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg<br /> 5641 taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt<br /> 5701 atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt<br /> 5761 tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc<br /> 5821 attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa<br /> 5881 atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat<br /> 5941 agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca<br /> 6001 tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt<br /> 6061 ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact<br /> 6121 tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca<br /> 6181 gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> 6241 cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat<br /> 6301 catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg<br /> 6361 atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc<br /> 6421 gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt<br /> 6481 gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt<br /> 6541 ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa<br /> 6601 ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc<br /> 6661 attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt<br /> 6721 cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata<br /> 6781 ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga<br /> 6841 cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc<br /> 6901 cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc<br /> 6961 acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat<br /> 7021 cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg<br /> 7081 ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag<br /> 7141 tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg<br /> 7201 ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt<br /> 7261 ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt<br /> 7321 tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac<br /> 7381 ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc<br /> 7441 agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca<br /> 7501 aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat<br /> 7561 ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag<br /> 7621 ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> 7681 tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta<br /> 7741 gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga<br /> 7801 ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg<br /> 7861 ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag<br /> 7921 ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta<br /> 7981 atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc<br /> 8041 gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc<br /> 8101 tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca<br /> 8161 agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga<br /> 8221 aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc<br /> 8281 atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa<br /> 8341 tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc<br /> 8401 tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa<br /> 8461 tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag<br /> 8521 cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca<br /> 8581 ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> 8641 ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga<br /> 8701 acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt<br /> 8761 tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg<br /> 8821 gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag<br /> 8881 gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag<br /> 8941 cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt<br /> 9001 gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga<br /> 9061 cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc<br /> 9121 tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact<br /> 9181 ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta<br /> 9241 agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg<br /> 9301 taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca<br /> 9361 agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc<br /> 9421 gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca<br /> 9481 aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca<br /> 9541 agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc<br /> 9601 acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc<br /> 9661 ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg<br /> 9721 ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg<br /> 9781 gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat<br /> 9841 aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc<br /> 9901 tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag<br /> 9961 ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt<br /> 10021 atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag<br /> 10081 agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt<br /> 10141 aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga<br /> 10201 aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc<br /> 10261 agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc<br /> 10321 cg&lt;/dnaseqJaponica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180397 AB013448 2014-06-08T00:55:21Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqJaponica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301# <br /> cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt<br /> 3361 tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt<br /> 3421 gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg<br /> 3481 cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg<br /> 3541 ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca<br /> 3601 aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct<br /> 3661 tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt<br /> 3721 ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga<br /> 3781 agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> 3841 ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca<br /> 3901 agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc<br /> 3961 acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg<br /> 4021 ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag<br /> 4081 tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg<br /> 4141 gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg<br /> 4201 tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag<br /> 4261 actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg<br /> 4321 agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga<br /> 4381 agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat<br /> 4441 gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg<br /> 4501 caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc<br /> 4561 agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat<br /> 4621 atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt<br /> 4681 gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc<br /> 4741 tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> 4801 agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg<br /> 4861 tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat<br /> 4921 tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat<br /> 4981 attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca<br /> 5041 aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt<br /> 5101 ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca<br /> 5161 aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga<br /> 5221 gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> 5281 caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg<br /> 5341 gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa<br /> 5401 aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat<br /> 5461 aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa<br /> 5521 ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat<br /> 5581 ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg<br /> 5641 taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt<br /> 5701 atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt<br /> 5761 tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc<br /> 5821 attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa<br /> 5881 atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat<br /> 5941 agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca<br /> 6001 tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt<br /> 6061 ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact<br /> 6121 tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca<br /> 6181 gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> 6241 cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat<br /> 6301 catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg<br /> 6361 atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc<br /> 6421 gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt<br /> 6481 gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt<br /> 6541 ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa<br /> 6601 ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc<br /> 6661 attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt<br /> 6721 cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata<br /> 6781 ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga<br /> 6841 cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc<br /> 6901 cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc<br /> 6961 acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat<br /> 7021 cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg<br /> 7081 ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag<br /> 7141 tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg<br /> 7201 ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt<br /> 7261 ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt<br /> 7321 tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac<br /> 7381 ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc<br /> 7441 agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca<br /> 7501 aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat<br /> 7561 ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag<br /> 7621 ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> 7681 tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta<br /> 7741 gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga<br /> 7801 ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg<br /> 7861 ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag<br /> 7921 ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta<br /> 7981 atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc<br /> 8041 gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc<br /> 8101 tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca<br /> 8161 agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga<br /> 8221 aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc<br /> 8281 atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa<br /> 8341 tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc<br /> 8401 tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa<br /> 8461 tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag<br /> 8521 cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca<br /> 8581 ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> 8641 ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga<br /> 8701 acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt<br /> 8761 tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg<br /> 8821 gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag<br /> 8881 gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag<br /> 8941 cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt<br /> 9001 gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga<br /> 9061 cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc<br /> 9121 tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact<br /> 9181 ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta<br /> 9241 agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg<br /> 9301 taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca<br /> 9361 agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc<br /> 9421 gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca<br /> 9481 aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca<br /> 9541 agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc<br /> 9601 acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc<br /> 9661 ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg<br /> 9721 ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg<br /> 9781 gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat<br /> 9841 aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc<br /> 9901 tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag<br /> 9961 ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt<br /> 10021 atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag<br /> 10081 agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt<br /> 10141 aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga<br /> 10201 aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc<br /> 10261 agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc<br /> 10321 cg&lt;/dnaseqJaponica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180396 AB013448 2014-06-08T00:43:55Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqJaponica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt<br /> 2461 catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc<br /> 2521 attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac<br /> 2581 agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg<br /> 2641 ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt<br /> 2701 actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct<br /> 2761 ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt<br /> 2821 atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> 2881 gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct<br /> 2941 attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt<br /> 3001 cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa<br /> 3061 gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac<br /> 3121 ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa<br /> 3181 aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag<br /> 3241 ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa<br /> 3301 ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt<br /> 3361 tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt<br /> 3421 gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg<br /> 3481 cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg<br /> 3541 ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca<br /> 3601 aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct<br /> 3661 tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt<br /> 3721 ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga<br /> 3781 agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> 3841 ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca<br /> 3901 agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc<br /> 3961 acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg<br /> 4021 ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag<br /> 4081 tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg<br /> 4141 gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg<br /> 4201 tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag<br /> 4261 actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg<br /> 4321 agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga<br /> 4381 agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat<br /> 4441 gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg<br /> 4501 caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc<br /> 4561 agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat<br /> 4621 atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt<br /> 4681 gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc<br /> 4741 tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> 4801 agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg<br /> 4861 tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat<br /> 4921 tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat<br /> 4981 attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca<br /> 5041 aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt<br /> 5101 ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca<br /> 5161 aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga<br /> 5221 gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> 5281 caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg<br /> 5341 gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa<br /> 5401 aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat<br /> 5461 aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa<br /> 5521 ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat<br /> 5581 ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg<br /> 5641 taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt<br /> 5701 atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt<br /> 5761 tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc<br /> 5821 attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa<br /> 5881 atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat<br /> 5941 agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca<br /> 6001 tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt<br /> 6061 ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact<br /> 6121 tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca<br /> 6181 gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> 6241 cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat<br /> 6301 catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg<br /> 6361 atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc<br /> 6421 gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt<br /> 6481 gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt<br /> 6541 ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa<br /> 6601 ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc<br /> 6661 attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt<br /> 6721 cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata<br /> 6781 ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga<br /> 6841 cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc<br /> 6901 cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc<br /> 6961 acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat<br /> 7021 cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg<br /> 7081 ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag<br /> 7141 tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg<br /> 7201 ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt<br /> 7261 ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt<br /> 7321 tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac<br /> 7381 ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc<br /> 7441 agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca<br /> 7501 aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat<br /> 7561 ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag<br /> 7621 ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> 7681 tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta<br /> 7741 gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga<br /> 7801 ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg<br /> 7861 ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag<br /> 7921 ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta<br /> 7981 atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc<br /> 8041 gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc<br /> 8101 tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca<br /> 8161 agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga<br /> 8221 aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc<br /> 8281 atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa<br /> 8341 tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc<br /> 8401 tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa<br /> 8461 tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag<br /> 8521 cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca<br /> 8581 ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> 8641 ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga<br /> 8701 acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt<br /> 8761 tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg<br /> 8821 gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag<br /> 8881 gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag<br /> 8941 cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt<br /> 9001 gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga<br /> 9061 cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc<br /> 9121 tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact<br /> 9181 ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta<br /> 9241 agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg<br /> 9301 taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca<br /> 9361 agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc<br /> 9421 gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca<br /> 9481 aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca<br /> 9541 agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc<br /> 9601 acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc<br /> 9661 ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg<br /> 9721 ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg<br /> 9781 gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat<br /> 9841 aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc<br /> 9901 tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag<br /> 9961 ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt<br /> 10021 atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag<br /> 10081 agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt<br /> 10141 aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga<br /> 10201 aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc<br /> 10261 agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc<br /> 10321 cg&lt;/dnaseqJaponica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180395 AB013448 2014-06-08T00:39:10Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqJaponica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg<br /> 1021 ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc<br /> 1081 agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc<br /> 1141 ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg<br /> 1201 ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg<br /> 1261 ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca<br /> 1321 ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac<br /> 1381 cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> 1441 gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga<br /> 1501 gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat<br /> 1561 agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct<br /> 1621 gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt<br /> 1681 ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt<br /> 1741 ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa<br /> 1801 ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat<br /> 1861 gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> 1921 gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc<br /> 1981 tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata<br /> 2041 gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta<br /> 2101 cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag<br /> 2161 gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt<br /> 2221 aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac<br /> 2281 tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> 2401 cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt<br /> 2461 catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc<br /> 2521 attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac<br /> 2581 agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg<br /> 2641 ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt<br /> 2701 actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct<br /> 2761 ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt<br /> 2821 atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> 2881 gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct<br /> 2941 attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt<br /> 3001 cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa<br /> 3061 gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac<br /> 3121 ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa<br /> 3181 aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag<br /> 3241 ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa<br /> 3301 ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt<br /> 3361 tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt<br /> 3421 gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg<br /> 3481 cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg<br /> 3541 ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca<br /> 3601 aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct<br /> 3661 tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt<br /> 3721 ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga<br /> 3781 agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> 3841 ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca<br /> 3901 agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc<br /> 3961 acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg<br /> 4021 ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag<br /> 4081 tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg<br /> 4141 gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg<br /> 4201 tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag<br /> 4261 actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg<br /> 4321 agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga<br /> 4381 agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat<br /> 4441 gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg<br /> 4501 caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc<br /> 4561 agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat<br /> 4621 atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt<br /> 4681 gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc<br /> 4741 tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> 4801 agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg<br /> 4861 tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat<br /> 4921 tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat<br /> 4981 attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca<br /> 5041 aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt<br /> 5101 ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca<br /> 5161 aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga<br /> 5221 gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> 5281 caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg<br /> 5341 gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa<br /> 5401 aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat<br /> 5461 aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa<br /> 5521 ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat<br /> 5581 ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg<br /> 5641 taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt<br /> 5701 atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt<br /> 5761 tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc<br /> 5821 attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa<br /> 5881 atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat<br /> 5941 agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca<br /> 6001 tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt<br /> 6061 ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact<br /> 6121 tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca<br /> 6181 gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> 6241 cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat<br /> 6301 catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg<br /> 6361 atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc<br /> 6421 gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt<br /> 6481 gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt<br /> 6541 ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa<br /> 6601 ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc<br /> 6661 attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt<br /> 6721 cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata<br /> 6781 ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga<br /> 6841 cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc<br /> 6901 cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc<br /> 6961 acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat<br /> 7021 cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg<br /> 7081 ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag<br /> 7141 tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg<br /> 7201 ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt<br /> 7261 ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt<br /> 7321 tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac<br /> 7381 ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc<br /> 7441 agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca<br /> 7501 aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat<br /> 7561 ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag<br /> 7621 ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> 7681 tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta<br /> 7741 gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga<br /> 7801 ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg<br /> 7861 ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag<br /> 7921 ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta<br /> 7981 atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc<br /> 8041 gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc<br /> 8101 tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca<br /> 8161 agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga<br /> 8221 aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc<br /> 8281 atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa<br /> 8341 tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc<br /> 8401 tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa<br /> 8461 tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag<br /> 8521 cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca<br /> 8581 ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> 8641 ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga<br /> 8701 acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt<br /> 8761 tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg<br /> 8821 gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag<br /> 8881 gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag<br /> 8941 cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt<br /> 9001 gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga<br /> 9061 cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc<br /> 9121 tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact<br /> 9181 ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta<br /> 9241 agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg<br /> 9301 taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca<br /> 9361 agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc<br /> 9421 gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca<br /> 9481 aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca<br /> 9541 agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc<br /> 9601 acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc<br /> 9661 ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg<br /> 9721 ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg<br /> 9781 gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat<br /> 9841 aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc<br /> 9901 tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag<br /> 9961 ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt<br /> 10021 atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag<br /> 10081 agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt<br /> 10141 aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga<br /> 10201 aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc<br /> 10261 agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc<br /> 10321 cg&lt;/dnaseqJaponica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180394 AB013448 2014-06-08T00:35:55Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqJaponica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca<br /> 421 gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt<br /> 481 ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa<br /> 541 gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac<br /> 601 ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc<br /> 661 gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat<br /> 721 ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa<br /> 781 caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag<br /> 841 gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt<br /> 901 tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta<br /> 961 caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg<br /> 1021 ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc<br /> 1081 agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc<br /> 1141 ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg<br /> 1201 ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg<br /> 1261 ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca<br /> 1321 ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac<br /> 1381 cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> 1441 gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga<br /> 1501 gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat<br /> 1561 agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct<br /> 1621 gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt<br /> 1681 ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt<br /> 1741 ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa<br /> 1801 ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat<br /> 1861 gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> 1921 gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc<br /> 1981 tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata<br /> 2041 gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta<br /> 2101 cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag<br /> 2161 gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt<br /> 2221 aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac<br /> 2281 tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> 2401 cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt<br /> 2461 catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc<br /> 2521 attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac<br /> 2581 agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg<br /> 2641 ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt<br /> 2701 actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct<br /> 2761 ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt<br /> 2821 atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> 2881 gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct<br /> 2941 attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt<br /> 3001 cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa<br /> 3061 gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac<br /> 3121 ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa<br /> 3181 aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag<br /> 3241 ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa<br /> 3301 ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt<br /> 3361 tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt<br /> 3421 gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg<br /> 3481 cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg<br /> 3541 ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca<br /> 3601 aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct<br /> 3661 tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt<br /> 3721 ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga<br /> 3781 agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> 3841 ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca<br /> 3901 agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc<br /> 3961 acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg<br /> 4021 ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag<br /> 4081 tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg<br /> 4141 gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg<br /> 4201 tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag<br /> 4261 actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg<br /> 4321 agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga<br /> 4381 agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat<br /> 4441 gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg<br /> 4501 caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc<br /> 4561 agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat<br /> 4621 atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt<br /> 4681 gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc<br /> 4741 tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> 4801 agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg<br /> 4861 tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat<br /> 4921 tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat<br /> 4981 attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca<br /> 5041 aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt<br /> 5101 ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca<br /> 5161 aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga<br /> 5221 gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> 5281 caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg<br /> 5341 gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa<br /> 5401 aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat<br /> 5461 aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa<br /> 5521 ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat<br /> 5581 ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg<br /> 5641 taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt<br /> 5701 atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt<br /> 5761 tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc<br /> 5821 attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa<br /> 5881 atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat<br /> 5941 agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca<br /> 6001 tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt<br /> 6061 ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact<br /> 6121 tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca<br /> 6181 gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> 6241 cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat<br /> 6301 catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg<br /> 6361 atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc<br /> 6421 gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt<br /> 6481 gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt<br /> 6541 ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa<br /> 6601 ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc<br /> 6661 attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt<br /> 6721 cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata<br /> 6781 ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga<br /> 6841 cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc<br /> 6901 cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc<br /> 6961 acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat<br /> 7021 cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg<br /> 7081 ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag<br /> 7141 tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg<br /> 7201 ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt<br /> 7261 ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt<br /> 7321 tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac<br /> 7381 ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc<br /> 7441 agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca<br /> 7501 aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat<br /> 7561 ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag<br /> 7621 ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> 7681 tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta<br /> 7741 gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga<br /> 7801 ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg<br /> 7861 ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag<br /> 7921 ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta<br /> 7981 atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc<br /> 8041 gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc<br /> 8101 tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca<br /> 8161 agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga<br /> 8221 aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc<br /> 8281 atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa<br /> 8341 tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc<br /> 8401 tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa<br /> 8461 tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag<br /> 8521 cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca<br /> 8581 ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> 8641 ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga<br /> 8701 acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt<br /> 8761 tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg<br /> 8821 gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag<br /> 8881 gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag<br /> 8941 cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt<br /> 9001 gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga<br /> 9061 cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc<br /> 9121 tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact<br /> 9181 ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta<br /> 9241 agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg<br /> 9301 taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca<br /> 9361 agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc<br /> 9421 gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca<br /> 9481 aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca<br /> 9541 agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc<br /> 9601 acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc<br /> 9661 ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg<br /> 9721 ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg<br /> 9781 gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat<br /> 9841 aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc<br /> 9901 tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag<br /> 9961 ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt<br /> 10021 atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag<br /> 10081 agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt<br /> 10141 aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga<br /> 10201 aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc<br /> 10261 agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc<br /> 10321 cg&lt;/dnaseqJaponica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180393 AB013448 2014-06-08T00:32:55Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqJaponica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg<br /> 241 ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt<br /> 301 agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt<br /> 361 gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca<br /> 421 gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt<br /> 481 ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa<br /> 541 gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac<br /> 601 ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc<br /> 661 gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat<br /> 721 ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa<br /> 781 caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag<br /> 841 gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt<br /> 901 tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta<br /> 961 caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg<br /> 1021 ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc<br /> 1081 agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc<br /> 1141 ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg<br /> 1201 ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg<br /> 1261 ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca<br /> 1321 ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac<br /> 1381 cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> 1441 gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga<br /> 1501 gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat<br /> 1561 agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct<br /> 1621 gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt<br /> 1681 ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt<br /> 1741 ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa<br /> 1801 ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat<br /> 1861 gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> 1921 gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc<br /> 1981 tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata<br /> 2041 gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta<br /> 2101 cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag<br /> 2161 gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt<br /> 2221 aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac<br /> 2281 tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc<br /> 2341 ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> 2401 cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt<br /> 2461 catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc<br /> 2521 attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac<br /> 2581 agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg<br /> 2641 ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt<br /> 2701 actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct<br /> 2761 ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt<br /> 2821 atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> 2881 gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct<br /> 2941 attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt<br /> 3001 cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa<br /> 3061 gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac<br /> 3121 ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa<br /> 3181 aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag<br /> 3241 ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa<br /> 3301 ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt<br /> 3361 tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt<br /> 3421 gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg<br /> 3481 cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg<br /> 3541 ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca<br /> 3601 aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct<br /> 3661 tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt<br /> 3721 ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga<br /> 3781 agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> 3841 ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca<br /> 3901 agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc<br /> 3961 acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg<br /> 4021 ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag<br /> 4081 tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg<br /> 4141 gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg<br /> 4201 tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag<br /> 4261 actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg<br /> 4321 agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga<br /> 4381 agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat<br /> 4441 gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg<br /> 4501 caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc<br /> 4561 agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat<br /> 4621 atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt<br /> 4681 gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc<br /> 4741 tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> 4801 agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg<br /> 4861 tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat<br /> 4921 tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat<br /> 4981 attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca<br /> 5041 aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt<br /> 5101 ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca<br /> 5161 aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga<br /> 5221 gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> 5281 caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg<br /> 5341 gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa<br /> 5401 aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat<br /> 5461 aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa<br /> 5521 ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat<br /> 5581 ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg<br /> 5641 taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt<br /> 5701 atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt<br /> 5761 tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc<br /> 5821 attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa<br /> 5881 atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat<br /> 5941 agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca<br /> 6001 tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt<br /> 6061 ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact<br /> 6121 tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca<br /> 6181 gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> 6241 cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat<br /> 6301 catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg<br /> 6361 atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc<br /> 6421 gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt<br /> 6481 gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt<br /> 6541 ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa<br /> 6601 ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc<br /> 6661 attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt<br /> 6721 cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata<br /> 6781 ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga<br /> 6841 cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc<br /> 6901 cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc<br /> 6961 acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat<br /> 7021 cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg<br /> 7081 ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag<br /> 7141 tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg<br /> 7201 ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt<br /> 7261 ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt<br /> 7321 tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac<br /> 7381 ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc<br /> 7441 agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca<br /> 7501 aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat<br /> 7561 ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag<br /> 7621 ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> 7681 tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta<br /> 7741 gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga<br /> 7801 ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg<br /> 7861 ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag<br /> 7921 ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta<br /> 7981 atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc<br /> 8041 gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc<br /> 8101 tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca<br /> 8161 agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga<br /> 8221 aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc<br /> 8281 atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa<br /> 8341 tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc<br /> 8401 tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa<br /> 8461 tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag<br /> 8521 cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca<br /> 8581 ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> 8641 ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga<br /> 8701 acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt<br /> 8761 tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg<br /> 8821 gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag<br /> 8881 gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag<br /> 8941 cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt<br /> 9001 gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga<br /> 9061 cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc<br /> 9121 tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact<br /> 9181 ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta<br /> 9241 agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg<br /> 9301 taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca<br /> 9361 agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc<br /> 9421 gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca<br /> 9481 aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca<br /> 9541 agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc<br /> 9601 acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc<br /> 9661 ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg<br /> 9721 ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg<br /> 9781 gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat<br /> 9841 aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc<br /> 9901 tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag<br /> 9961 ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt<br /> 10021 atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag<br /> 10081 agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt<br /> 10141 aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga<br /> 10201 aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc<br /> 10261 agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc<br /> 10321 cg&lt;/dnaseqJaponica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180392 AB013448 2014-06-08T00:27:57Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqJaponica&gt;1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341#<br /> 1 cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat<br /> 61 actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact<br /> 121 agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg<br /> 181 tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg<br /> 241 ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt<br /> 301 agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt<br /> 361 gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca<br /> 421 gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt<br /> 481 ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa<br /> 541 gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac<br /> 601 ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc<br /> 661 gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat<br /> 721 ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa<br /> 781 caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag<br /> 841 gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt<br /> 901 tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta<br /> 961 caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg<br /> 1021 ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc<br /> 1081 agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc<br /> 1141 ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg<br /> 1201 ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg<br /> 1261 ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca<br /> 1321 ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac<br /> 1381 cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat<br /> 1441 gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga<br /> 1501 gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat<br /> 1561 agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct<br /> 1621 gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt<br /> 1681 ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt<br /> 1741 ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa<br /> 1801 ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat<br /> 1861 gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta<br /> 1921 gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc<br /> 1981 tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata<br /> 2041 gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta<br /> 2101 cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag<br /> 2161 gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt<br /> 2221 aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac<br /> 2281 tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc<br /> 2341 ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag<br /> 2401 cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt<br /> 2461 catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc<br /> 2521 attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac<br /> 2581 agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg<br /> 2641 ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt<br /> 2701 actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct<br /> 2761 ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt<br /> 2821 atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga<br /> 2881 gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct<br /> 2941 attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt<br /> 3001 cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa<br /> 3061 gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac<br /> 3121 ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa<br /> 3181 aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag<br /> 3241 ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa<br /> 3301 ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt<br /> 3361 tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt<br /> 3421 gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg<br /> 3481 cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg<br /> 3541 ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca<br /> 3601 aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct<br /> 3661 tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt<br /> 3721 ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga<br /> 3781 agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc<br /> 3841 ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca<br /> 3901 agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc<br /> 3961 acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg<br /> 4021 ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag<br /> 4081 tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg<br /> 4141 gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg<br /> 4201 tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag<br /> 4261 actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg<br /> 4321 agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga<br /> 4381 agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat<br /> 4441 gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg<br /> 4501 caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc<br /> 4561 agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat<br /> 4621 atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt<br /> 4681 gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc<br /> 4741 tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac<br /> 4801 agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg<br /> 4861 tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat<br /> 4921 tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat<br /> 4981 attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca<br /> 5041 aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt<br /> 5101 ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca<br /> 5161 aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga<br /> 5221 gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc<br /> 5281 caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg<br /> 5341 gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa<br /> 5401 aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat<br /> 5461 aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa<br /> 5521 ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat<br /> 5581 ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg<br /> 5641 taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt<br /> 5701 atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt<br /> 5761 tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc<br /> 5821 attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa<br /> 5881 atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat<br /> 5941 agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca<br /> 6001 tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt<br /> 6061 ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact<br /> 6121 tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca<br /> 6181 gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag<br /> 6241 cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat<br /> 6301 catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg<br /> 6361 atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc<br /> 6421 gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt<br /> 6481 gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt<br /> 6541 ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa<br /> 6601 ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc<br /> 6661 attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt<br /> 6721 cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata<br /> 6781 ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga<br /> 6841 cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc<br /> 6901 cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc<br /> 6961 acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat<br /> 7021 cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg<br /> 7081 ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag<br /> 7141 tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg<br /> 7201 ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt<br /> 7261 ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt<br /> 7321 tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac<br /> 7381 ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc<br /> 7441 agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca<br /> 7501 aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat<br /> 7561 ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag<br /> 7621 ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt<br /> 7681 tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta<br /> 7741 gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga<br /> 7801 ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg<br /> 7861 ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag<br /> 7921 ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta<br /> 7981 atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc<br /> 8041 gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc<br /> 8101 tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca<br /> 8161 agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga<br /> 8221 aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc<br /> 8281 atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa<br /> 8341 tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc<br /> 8401 tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa<br /> 8461 tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag<br /> 8521 cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca<br /> 8581 ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc<br /> 8641 ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga<br /> 8701 acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt<br /> 8761 tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg<br /> 8821 gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag<br /> 8881 gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag<br /> 8941 cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt<br /> 9001 gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga<br /> 9061 cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc<br /> 9121 tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact<br /> 9181 ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta<br /> 9241 agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg<br /> 9301 taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca<br /> 9361 agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc<br /> 9421 gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca<br /> 9481 aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca<br /> 9541 agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc<br /> 9601 acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc<br /> 9661 ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg<br /> 9721 ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg<br /> 9781 gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat<br /> 9841 aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc<br /> 9901 tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag<br /> 9961 ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt<br /> 10021 atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag<br /> 10081 agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt<br /> 10141 aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga<br /> 10201 aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc<br /> 10261 agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc<br /> 10321 cg&lt;/dnaseqJaponica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/4521189]|<br /> }}</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180391 AB013448 2014-06-08T00:23:11Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:2293..9202|<br /> CDS = 2293..9202|<br /> AA = &lt;aaseq&gt;MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE<br /> LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL<br /> LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR<br /> RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA<br /> WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN<br /> DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA<br /> FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR<br /> TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR<br /> LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE<br /> LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS<br /> GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI<br /> VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY<br /> LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS<br /> SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE<br /> FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF<br /> LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW<br /> EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH<br /> EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR<br /> LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL<br /> VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL<br /> AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR<br /> IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT&lt;/aaseq&gt;|</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180390 AB013448 2014-06-08T00:02:18Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)<br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180054 AB013448 2014-06-07T10:25:32Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|<br /> Version = AB013448.1 GI:4521189|<br /> Length = 10322 bp|<br /> Definition = Oryza sativa Japonica Group gene for Pib, complete cds.|<br /> Source = Oryza sativa Japonica Group (Japanese rice)</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180050 AB013448 2014-06-07T10:19:57Z <p>Jirongli: /* Structured Information */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = AB013448|<br /> Description = 10322 bp DNA linear PLN 15-FEB-2008.|</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180043 AB013448 2014-06-07T10:01:21Z <p>Jirongli: /* 分子标记辅助选择育种 */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ====分子标记辅助选择育种====<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180041 AB013448 2014-06-07T10:01:06Z <p>Jirongli: /* 基因克隆与生物学功能分析 */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ====基因克隆与生物学功能分析====<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ==分子标记辅助选择育种==<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180040 AB013448 2014-06-07T10:00:53Z <p>Jirongli: /* 基因的定位 */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ====基因的定位====<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ==基因克隆与生物学功能分析==<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ==分子标记辅助选择育种==<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180039 AB013448 2014-06-07T10:00:37Z <p>Jirongli: /* 常规信息 */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ====常规信息====<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ==基因的定位==<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ==基因克隆与生物学功能分析==<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ==分子标记辅助选择育种==<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180038 AB013448 2014-06-07T09:58:50Z <p>Jirongli: /* Function */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> ==常规信息==<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> ==基因的定位==<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ==基因克隆与生物学功能分析==<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ==分子标记辅助选择育种==<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180036 AB013448 2014-06-07T09:57:04Z <p>Jirongli: /* 【分子标记辅助选择育种】 */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> <br /> ==基因的定位==<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ==基因克隆与生物学功能分析==<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ==分子标记辅助选择育种==<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180035 AB013448 2014-06-07T09:56:46Z <p>Jirongli: /* 【基因克隆与生物学功能分析】 */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> <br /> ==基因的定位==<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ==基因克隆与生物学功能分析==<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ===【分子标记辅助选择育种】===<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180034 AB013448 2014-06-07T09:56:21Z <p>Jirongli: /* 【基因的定位】 */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> <br /> ==基因的定位==<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ===【基因克隆与生物学功能分析】===<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ===【分子标记辅助选择育种】===<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180032 AB013448 2014-06-07T09:55:27Z <p>Jirongli: /* Function */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> <br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> <br /> ===【基因的定位】===<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> ===【基因克隆与生物学功能分析】===<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> ===【分子标记辅助选择育种】===<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180027 AB013448 2014-06-07T09:52:50Z <p>Jirongli: /* Function */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> <br /> 【基因的定位】<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> <br /> 【基因克隆与生物学功能分析】<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> <br /> 【分子标记辅助选择育种】<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180026 AB013448 2014-06-07T09:51:25Z <p>Jirongli: /* Function */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> Pib ，水稻稻瘟病抗性基因，来自品种&quot;BL1&quot;，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。<br /> 【基因的定位】<br /> Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。<br /> Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。<br /> 【基因克隆与生物学功能分析】<br /> Pib 基因属于&quot;NBS-LRR&quot;类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。<br /> Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。<br /> 【分子标记辅助选择育种】<br /> 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180022 AB013448 2014-06-07T09:48:44Z <p>Jirongli: /* Function */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (&lt;0.5 and &lt;1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180020 AB013448 2014-06-07T09:47:38Z <p>Jirongli: /* Expression */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Expression===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180018 AB013448 2014-06-07T09:45:58Z <p>Jirongli: /* Evolution */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180017 AB013448 2014-06-07T09:43:06Z <p>Jirongli: /* References */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180016 AB013448 2014-06-07T09:42:37Z <p>Jirongli: /* Function */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> Please input cited references here.<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180014 AB013448 2014-06-07T09:39:41Z <p>Jirongli: /* Function */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> Please input cited references here.<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180010 AB013448 2014-06-07T09:36:35Z <p>Jirongli: /* References */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> Please input cited references here.<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> <br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> <br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> <br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> <br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180008 AB013448 2014-06-07T09:35:49Z <p>Jirongli: /* References */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> Please input cited references here.<br /> <br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675<br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14<br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64<br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli https://ngdc.cncb.ac.cn/ricewiki/index.php?title=AB013448&diff=180007 AB013448 2014-06-07T09:33:37Z <p>Jirongli: /* References */</p> <hr /> <div>==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> ==References==<br /> Please input cited references here.<br /> 1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta<br /> Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.)<br /> Plant Breeding, 2010, 129(6): 670-675<br /> 2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军<br /> 水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用<br /> 中国农业科学, 2008, 41(1): 9-14<br /> 3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki<br /> The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes<br /> The Plant Journal, 1999, 19(1): 55-64<br /> 4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki<br /> Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b<br /> Theoretical and Applied Genetics, 1997, 94(2): 170-176<br /> 5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki<br /> High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b<br /> Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13<br /> <br /> ==Structured Information==</div> Jirongli