http://192.168.164.12:81/ricewiki/api.php?action=feedcontributions&feedformat=atom&user=LIZHAO 2026-09-07T05:16:42Z User contributions MediaWiki 1.30.0 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:LIZHAO1.png&diff=183198 2014-06-10T03:48:52Z

<p>LIZHAO: uploaded a new version of &amp;quot;File:LIZHAO1.png&amp;quot;: Hypothetical model of the regulation of OsAMT1 genes in rice roots.</p> <hr /> <div> Hypothetical model of the regulation of OsAMT1 genes in rice roots.</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:LIZHAO5.png&diff=183197 2014-06-10T03:47:14Z

<p>LIZHAO: A phylogenetic tree of ammonium transporters in higher plants.</p> <hr /> <div>A phylogenetic tree of ammonium transporters in higher plants.</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0620600&diff=183196 2014-06-10T03:46:44Z

<p>LIZHAO: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> # AmmoniAmmonium transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.um transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.&lt;ref name=&quot;ref1&quot; /&gt;<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. Southern blot analysis and screening of the rice genome database confirmed that with OsAMT1;1–1;3the complete AMT1 family of rice had been isolated. &lt;ref name=&quot;ref2&quot; /&gt;<br /> #The amino acid sequences of the OsAMT1;1, OsAMT1;2 and OsAMT1;3 share high sequence similarity to each other and to other AMT1-type ammonium transporters previously identified from other plant species. All three isolated OsAMT1 genes encode functional ammonium transporters.Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3.<br /> #OsAMT1;2 has ammonium transporters activity and mediates ammonium uptake from the soil solution. &lt;ref name=&quot;ref3&quot; /&gt;<br /> <br /> ===Expression===<br /> # OsAMT1;2 is expressed in the central cylinder and cell surface of root tips.<br /> # OsAMT1;2 expression was root specific and ammonium inducible.&lt;ref name=&quot;ref2&quot; /&gt;<br /> # Ammonium showed positive effects on the expression of OsAMT1;2. These effects were mimicked not only by glutamine but also by asparagines. On the other hand,glutamate and aspartate were ineffective with respect to OsAMT1;2.('''Figure 1''').&lt;ref name=&quot;ref3&quot; /&gt;[[File:LIZHAO1.png‎|frame|'''Figure 1'''Hypothetical model of the regulation of OsAMT1 genes in rice roots.]]<br /> OsAMT1gene expression is positively feedback-regulated (dotted arrow) and negatively feedback-regulated (dotted T line) by cytosolic glutamine(Gln). Nitrogen fluxes are shown as solid arrows. See text for details.<br /> <br /> While OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3). while OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3). We complemented a yeast mutant to determine whether the protein encoded by the cDNA is a functional ammonium transporter. Both cDNAs were able to functionally complement the mutant which is deficient in ammonium uptake, indicating that OsAMT1;1 and OsAMT1;2 encodes functional ammonium transporters (data not shown). Thus, OsAMT1;1 and OsAMT1;2 may have different physiological roles in ammonium uptake in rice. There results imply that OsAMT1;1 is involved in uptake and/or salvage of the ammonium in vascular tissues, and OsAMT1;2 in the uptake in root epidermis. Further experiments will be needeed to evaluate this hypothesis.&lt;ref name=&quot;ref4&quot; /&gt;<br /> [[File:LIZHAO2.png‎|frame|'''Figure 2'''Figs. 2 .]]<br /> [[File:LIZHAO3.png‎|frame|'''Figure 2'''Figs. 3 .]]<br /> <br /> ===Evolution===<br /> # Diversity and selective sweep in the OsAMT1;2 genomic region of rice. AMT1;2 transgenic rice plants with enhanced NH4+ permeability show superior growth and higher yield under optimal and suboptimal NH4+ conditions. <br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. ('''Figure4''').&lt;ref name=&quot;ref1&quot; /&gt;<br /> [[File:LIZHAO4.png‎|frame|'''Figure4'''Localization of OsAMT1;2mRNA in rice root tip by in situ hybridization.]]<br /> # Hydropathy analysis of the deduced amino acid sequence suggests that OsAMT1;1 and OsAMT1;2 contain 10-11 putative trans-membrane domains. Sequence comparison shows that OsAMT1;1 and OsAMT1;2 are closely related to Arabidopsis and tomato ammonium transporters (67-63%,). ('''Figure5''').&lt;ref name=&quot;ref5&quot; /&gt;<br /> [[File:LIZHAO5.png‎|frame|'''Figure5''']]<br /> <br /> ==Labs working on this gene==<br /> # BioScience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan<br /> # State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China)<br /> # Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences,Hunan 410125 (China)<br /> # Department of Botany, the University of British Columbia, Vancouver, BC, Canada <br /> # Agriculture and AgriFood Canada, Shelterbelt Centre Indian Head SK, Canada <br /> # Division of Biology, University of California at San Diego, La Jolla, CA 92093–0116, USA<br /> # Division of Biological Sciences, Graduate School of Science, Hokkaido University, Kita-ku N10-W8, Sapporo, 060-0810 Japan <br /> # CREST, Japan Science and Technology Corporation (JST)CREST, Japan Science and Technology Corporation (JST)<br /> # Division of Bioresources and Product Science, Graduate School of Agriculture, Hokkaido University, Kita-ku N9-W9, Sapporo, 060-8589 Japan <br /> # Plant Science Center, RIKEN, 1-7-22 Suehiro, Tsurumi-ku, Yokohama, 230-0045 Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Sonoda Y, Ikeda A, Yamaya T, et al. Feedback regulation of the ammonium transporter gene family AMT1 by glutamine in rice[C]//Plant and Cell Physiology. GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND: OXFORD UNIV PRESS, 2004, 45: S98-S98.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Suenaga A, Moriya K, Sonoda Y, et al. Constitutive expression of a novel-type ammonium transporter OsAMT2 in rice plants[J]. Plant and Cell Physiology, 2003, 44(2): 206-211.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref3&quot;&gt;Yutaka Sonoda; Akira Ikeda; Satomi Saiki, et al. Expression and Function of Three Ammonium Transporter Genes (OsAMT1;1 – 1;3) in Rice[C]// Plant and Cell Physiology, 2003, 44(7): 726-734.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref4&quot;&gt;Lauter F.R. O. Ninnemann, M. Bucher, J.W. Riesmeier and W.B. Frommer, 1996. Preferential expression of an ammonium transporter and of two putative nitrate transporters in root hairs of tomato[C]//Proc. Natl. Acad. Sci. USA 93: 8139-8144. &lt;/ref&gt;<br /> &lt;ref name=&quot;ref5&quot;&gt;Y. SONODA, A. IKEDA and J. YAMAGUCHI Characterization of rice ammonium transporters, OsAMT1;1 and OsAMT1;2[C]// Rice Genetics Newsletter, Vol. 17.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0620600|<br /> Description = Ammonium transporter family protein|<br /> Version = NM_001053991.1 GI:115447352 GeneID:4330008|<br /> Length = 1853 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0620600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:25548745..25550597|<br /> CDS = 25548816..25550306|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATCADTLGPLLGTAAANATDYLCNQFADTTSAVDSTYLLFSAY LVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGAPSNGFIG KHFFGLKQVPQVGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVY PVVSHWIWSADGWASASRTSGSLLFGSGVIDFAGSGVVHMVGGVAGLWGALIEGPRIG RFDHAGRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTILKSYGPPGSIHGQWSAV GRTAVTTTLAGSTAALTTLFGKRLQTGHWNVIDVCNGLLGGFAAITAGCSVVDPWAAI ICGFVSAWVLIGLNALAARLKFDDPLEAAQLHGGCGAWGVIFTALFARKEYVDQIFGQ PGRPYGLFMGGGGRLLGAHIVVILVIAAWVSFTMAPLFLVLNKLGLLRISAEDEMAGM DQTRHGGFAYAYHDDDASGKPDRSVGGFMLKSAHGTQVAAEMGGHV&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;72..1562#acagcccacacttccattgctcctcccctctcctctacagtctgtgttgagcgcgcgtcgaggcggcgaggatggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctagtggaaccggaggagctgagctagtagtacatacatgcagcatcatcgatcgaacgaaatgcatataagcgtttttcaaggttgatctgatgctgcaggtttcgtgattgtataataggaagaaaaagatagtagtattttttatctgagatcatctgtttggaacaggggatttgactaagatttgatataaatttacacaaaatcttagcaaaaatccctttatctcaactctcaagtagagctttgctttgtacaacaaagtatcatgtgtgatataattgtcaggtgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053991.1 RefSeq:Os02g0620600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:LIZHAO4.png&diff=183194 2014-06-10T03:45:50Z

<p>LIZHAO: Localization of OsAMT1;2mRNA in rice root tip by in situ hybridization</p> <hr /> <div>Localization of OsAMT1;2mRNA in rice root tip by in situ hybridization</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:LIZHAO3.png&diff=183193 2014-06-10T03:44:55Z

<p>LIZHAO: Fig.3</p> <hr /> <div>Fig.3</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:LIZHAO2.png&diff=183192 2014-06-10T03:44:18Z

<p>LIZHAO: Fig.2</p> <hr /> <div>Fig.2</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0620600&diff=183191 2014-06-10T03:41:07Z

<p>LIZHAO: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> # AmmoniAmmonium transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.um transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.&lt;ref name=&quot;ref1&quot; /&gt;<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. Southern blot analysis and screening of the rice genome database confirmed that with OsAMT1;1–1;3the complete AMT1 family of rice had been isolated. &lt;ref name=&quot;ref2&quot; /&gt;<br /> #The amino acid sequences of the OsAMT1;1, OsAMT1;2 and OsAMT1;3 share high sequence similarity to each other and to other AMT1-type ammonium transporters previously identified from other plant species. All three isolated OsAMT1 genes encode functional ammonium transporters.Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3.<br /> #OsAMT1;2 has ammonium transporters activity and mediates ammonium uptake from the soil solution. &lt;ref name=&quot;ref3&quot; /&gt;<br /> <br /> ===Expression===<br /> # OsAMT1;2 is expressed in the central cylinder and cell surface of root tips.<br /> # OsAMT1;2 expression was root specific and ammonium inducible.&lt;ref name=&quot;ref2&quot; /&gt;<br /> # Ammonium showed positive effects on the expression of OsAMT1;2. These effects were mimicked not only by glutamine but also by asparagines. On the other hand,glutamate and aspartate were ineffective with respect to OsAMT1;2.('''Figure 1''').&lt;ref name=&quot;ref3&quot; /&gt;[[File:LIZHAO1.png‎|frame|'''Figure 1'''Hypothetical model of the regulation of OsAMT1 genes in rice roots.]]<br /> OsAMT1gene expression is positively feedback-regulated (dotted arrow) and negatively feedback-regulated (dotted T line) by cytosolic glutamine(Gln). Nitrogen fluxes are shown as solid arrows. See text for details.<br /> <br /> While OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3). while OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3). We complemented a yeast mutant to determine whether the protein encoded by the cDNA is a functional ammonium transporter. Both cDNAs were able to functionally complement the mutant which is deficient in ammonium uptake, indicating that OsAMT1;1 and OsAMT1;2 encodes functional ammonium transporters (data not shown). Thus, OsAMT1;1 and OsAMT1;2 may have different physiological roles in ammonium uptake in rice. There results imply that OsAMT1;1 is involved in uptake and/or salvage of the ammonium in vascular tissues, and OsAMT1;2 in the uptake in root epidermis. Further experiments will be needeed to evaluate this hypothesis.&lt;ref name=&quot;ref4&quot; /&gt;<br /> [[File:LIZHAO2.png‎|frame|'''Figure 2'''Figs. 2 .]]<br /> [[File:LIZHAO3.png‎|frame|'''Figure 2'''Figs. 3 .]]<br /> <br /> ===Evolution===<br /> # Diversity and selective sweep in the OsAMT1;2 genomic region of rice. AMT1;2 transgenic rice plants with enhanced NH4+ permeability show superior growth and higher yield under optimal and suboptimal NH4+ conditions. <br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. ('''Figure4''').&lt;ref name=&quot;ref1&quot; /&gt;<br /> [[File:LIZHAO4.jpg‎|frame|'''Figure4'''Localization of OsAMT1;2mRNA in rice root tip by in situ hybridization.]]<br /> # Hydropathy analysis of the deduced amino acid sequence suggests that OsAMT1;1 and OsAMT1;2 contain 10-11 putative trans-membrane domains. Sequence comparison shows that OsAMT1;1 and OsAMT1;2 are closely related to Arabidopsis and tomato ammonium transporters (67-63%,). ('''Figure5''').&lt;ref name=&quot;ref5&quot; /&gt;<br /> [[File:LIZHAO5.jpg‎|frame|'''Figure5''']]<br /> <br /> ==Labs working on this gene==<br /> # BioScience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan<br /> # State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China)<br /> # Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences,Hunan 410125 (China)<br /> # Department of Botany, the University of British Columbia, Vancouver, BC, Canada <br /> # Agriculture and AgriFood Canada, Shelterbelt Centre Indian Head SK, Canada <br /> # Division of Biology, University of California at San Diego, La Jolla, CA 92093–0116, USA<br /> # Division of Biological Sciences, Graduate School of Science, Hokkaido University, Kita-ku N10-W8, Sapporo, 060-0810 Japan <br /> # CREST, Japan Science and Technology Corporation (JST)CREST, Japan Science and Technology Corporation (JST)<br /> # Division of Bioresources and Product Science, Graduate School of Agriculture, Hokkaido University, Kita-ku N9-W9, Sapporo, 060-8589 Japan <br /> # Plant Science Center, RIKEN, 1-7-22 Suehiro, Tsurumi-ku, Yokohama, 230-0045 Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Sonoda Y, Ikeda A, Yamaya T, et al. Feedback regulation of the ammonium transporter gene family AMT1 by glutamine in rice[C]//Plant and Cell Physiology. GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND: OXFORD UNIV PRESS, 2004, 45: S98-S98.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Suenaga A, Moriya K, Sonoda Y, et al. Constitutive expression of a novel-type ammonium transporter OsAMT2 in rice plants[J]. Plant and Cell Physiology, 2003, 44(2): 206-211.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref3&quot;&gt;Yutaka Sonoda; Akira Ikeda; Satomi Saiki, et al. Expression and Function of Three Ammonium Transporter Genes (OsAMT1;1 – 1;3) in Rice[C]// Plant and Cell Physiology, 2003, 44(7): 726-734.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref4&quot;&gt;Lauter F.R. O. Ninnemann, M. Bucher, J.W. Riesmeier and W.B. Frommer, 1996. Preferential expression of an ammonium transporter and of two putative nitrate transporters in root hairs of tomato[C]//Proc. Natl. Acad. Sci. USA 93: 8139-8144. &lt;/ref&gt;<br /> &lt;ref name=&quot;ref5&quot;&gt;Y. SONODA, A. IKEDA and J. YAMAGUCHI Characterization of rice ammonium transporters, OsAMT1;1 and OsAMT1;2[C]// Rice Genetics Newsletter, Vol. 17.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0620600|<br /> Description = Ammonium transporter family protein|<br /> Version = NM_001053991.1 GI:115447352 GeneID:4330008|<br /> Length = 1853 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0620600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:25548745..25550597|<br /> CDS = 25548816..25550306|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATCADTLGPLLGTAAANATDYLCNQFADTTSAVDSTYLLFSAY LVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGAPSNGFIG KHFFGLKQVPQVGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVY PVVSHWIWSADGWASASRTSGSLLFGSGVIDFAGSGVVHMVGGVAGLWGALIEGPRIG RFDHAGRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTILKSYGPPGSIHGQWSAV GRTAVTTTLAGSTAALTTLFGKRLQTGHWNVIDVCNGLLGGFAAITAGCSVVDPWAAI ICGFVSAWVLIGLNALAARLKFDDPLEAAQLHGGCGAWGVIFTALFARKEYVDQIFGQ PGRPYGLFMGGGGRLLGAHIVVILVIAAWVSFTMAPLFLVLNKLGLLRISAEDEMAGM DQTRHGGFAYAYHDDDASGKPDRSVGGFMLKSAHGTQVAAEMGGHV&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;72..1562#acagcccacacttccattgctcctcccctctcctctacagtctgtgttgagcgcgcgtcgaggcggcgaggatggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctagtggaaccggaggagctgagctagtagtacatacatgcagcatcatcgatcgaacgaaatgcatataagcgtttttcaaggttgatctgatgctgcaggtttcgtgattgtataataggaagaaaaagatagtagtattttttatctgagatcatctgtttggaacaggggatttgactaagatttgatataaatttacacaaaatcttagcaaaaatccctttatctcaactctcaagtagagctttgctttgtacaacaaagtatcatgtgtgatataattgtcaggtgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053991.1 RefSeq:Os02g0620600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0620600&diff=183190 2014-06-10T03:40:27Z

<p>LIZHAO: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> # AmmoniAmmonium transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.um transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.&lt;ref name=&quot;ref1&quot; /&gt;<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. Southern blot analysis and screening of the rice genome database confirmed that with OsAMT1;1–1;3the complete AMT1 family of rice had been isolated. &lt;ref name=&quot;ref2&quot; /&gt;<br /> #The amino acid sequences of the OsAMT1;1, OsAMT1;2 and OsAMT1;3 share high sequence similarity to each other and to other AMT1-type ammonium transporters previously identified from other plant species. All three isolated OsAMT1 genes encode functional ammonium transporters.Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3.<br /> #OsAMT1;2 has ammonium transporters activity and mediates ammonium uptake from the soil solution. &lt;ref name=&quot;ref3&quot; /&gt;<br /> <br /> ===Expression===<br /> # OsAMT1;2 is expressed in the central cylinder and cell surface of root tips.<br /> # OsAMT1;2 expression was root specific and ammonium inducible.&lt;ref name=&quot;ref2&quot; /&gt;<br /> # Ammonium showed positive effects on the expression of OsAMT1;2. These effects were mimicked not only by glutamine but also by asparagines. On the other hand,glutamate and aspartate were ineffective with respect to OsAMT1;2.('''Figure 1''').&lt;ref name=&quot;ref3&quot; /&gt;[[File:LIZHAO1.png‎|frame|'''Figure 1'''Hypothetical model of the regulation of OsAMT1 genes in rice roots.]]<br /> OsAMT1gene expression is positively feedback-regulated (dotted arrow) and negatively feedback-regulated (dotted T line) by cytosolic glutamine(Gln). Nitrogen fluxes are shown as solid arrows. See text for details.<br /> <br /> While OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3). while OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3). We complemented a yeast mutant to determine whether the protein encoded by the cDNA is a functional ammonium transporter. Both cDNAs were able to functionally complement the mutant which is deficient in ammonium uptake, indicating that OsAMT1;1 and OsAMT1;2 encodes functional ammonium transporters (data not shown). Thus, OsAMT1;1 and OsAMT1;2 may have different physiological roles in ammonium uptake in rice. There results imply that OsAMT1;1 is involved in uptake and/or salvage of the ammonium in vascular tissues, and OsAMT1;2 in the uptake in root epidermis. Further experiments will be needeed to evaluate this hypothesis.&lt;ref name=&quot;ref4&quot; /&gt;<br /> [[File:LIZHAO2.png‎|frame|'''Figure 2'''Figs. 2 .]]<br /> [[File:LIZHAO3.png‎|frame|'''Figure 2'''Figs. 3 .]]<br /> <br /> ===Evolution===<br /> # Diversity and selective sweep in the OsAMT1;2 genomic region of rice. AMT1;2 transgenic rice plants with enhanced NH4+ permeability show superior growth and higher yield under optimal and suboptimal NH4+ conditions. <br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. ('''Figure4''').&lt;ref name=&quot;ref1&quot; /&gt;<br /> [[File:LIZHAO4.jpg‎|frame|'''Figure4'''Localization of OsAMT1;2mRNA in rice root tip by in situ hybridization.]]<br /> # Hydropathy analysis of the deduced amino acid sequence suggests that OsAMT1;1 and OsAMT1;2 contain 10-11 putative trans-membrane domains. Sequence comparison shows that OsAMT1;1 and OsAMT1;2 are closely related to Arabidopsis and tomato ammonium transporters (67-63%,). ('''Figure5''').&lt;ref name=&quot;ref5&quot; /&gt;<br /> [[File:LIZHAO5.jpg‎|frame|'''Figure5''']]<br /> <br /> ==Labs working on this gene==<br /> # BioScience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan<br /> # State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China)<br /> # Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences,Hunan 410125 (China)<br /> # Department of Botany, the University of British Columbia, Vancouver, BC, Canada <br /> # Agriculture and AgriFood Canada, Shelterbelt Centre Indian Head SK, Canada <br /> # Division of Biology, University of California at San Diego, La Jolla, CA 92093–0116, USA<br /> # Division of Biological Sciences, Graduate School of Science, Hokkaido University, Kita-ku N10-W8, Sapporo, 060-0810 Japan <br /> # CREST, Japan Science and Technology Corporation (JST)CREST, Japan Science and Technology Corporation (JST)<br /> # Division of Bioresources and Product Science, Graduate School of Agriculture, Hokkaido University, Kita-ku N9-W9, Sapporo, 060-8589 Japan <br /> # Plant Science Center, RIKEN, 1-7-22 Suehiro, Tsurumi-ku, Yokohama, 230-0045 Japan<br /> <br /> ==References==<br /> &lt;ref name=&quot;ref1&quot;&gt;Sonoda Y, Ikeda A, Yamaya T, et al. Feedback regulation of the ammonium transporter gene family AMT1 by glutamine in rice[C]//Plant and Cell Physiology. GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND: OXFORD UNIV PRESS, 2004, 45: S98-S98.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Suenaga A, Moriya K, Sonoda Y, et al. Constitutive expression of a novel-type ammonium transporter OsAMT2 in rice plants[J]. Plant and Cell Physiology, 2003, 44(2): 206-211.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref3&quot;&gt;Yutaka Sonoda; Akira Ikeda; Satomi Saiki, et al. Expression and Function of Three Ammonium Transporter Genes (OsAMT1;1 – 1;3) in Rice[C]// Plant and Cell Physiology, 2003, 44(7): 726-734.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref4&quot;&gt;Lauter F.R. O. Ninnemann, M. Bucher, J.W. Riesmeier and W.B. Frommer, 1996. Preferential expression of an ammonium transporter and of two putative nitrate transporters in root hairs of tomato[C]//Proc. Natl. Acad. Sci. USA 93: 8139-8144. &lt;/ref&gt;<br /> &lt;ref name=&quot;ref5&quot;&gt;Y. SONODA, A. IKEDA and J. YAMAGUCHI Characterization of rice ammonium transporters, OsAMT1;1 and OsAMT1;2[C]// Rice Genetics Newsletter, Vol. 17.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0620600|<br /> Description = Ammonium transporter family protein|<br /> Version = NM_001053991.1 GI:115447352 GeneID:4330008|<br /> Length = 1853 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0620600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:25548745..25550597|<br /> CDS = 25548816..25550306|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATCADTLGPLLGTAAANATDYLCNQFADTTSAVDSTYLLFSAY LVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGAPSNGFIG KHFFGLKQVPQVGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVY PVVSHWIWSADGWASASRTSGSLLFGSGVIDFAGSGVVHMVGGVAGLWGALIEGPRIG RFDHAGRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTILKSYGPPGSIHGQWSAV GRTAVTTTLAGSTAALTTLFGKRLQTGHWNVIDVCNGLLGGFAAITAGCSVVDPWAAI ICGFVSAWVLIGLNALAARLKFDDPLEAAQLHGGCGAWGVIFTALFARKEYVDQIFGQ PGRPYGLFMGGGGRLLGAHIVVILVIAAWVSFTMAPLFLVLNKLGLLRISAEDEMAGM DQTRHGGFAYAYHDDDASGKPDRSVGGFMLKSAHGTQVAAEMGGHV&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;72..1562#acagcccacacttccattgctcctcccctctcctctacagtctgtgttgagcgcgcgtcgaggcggcgaggatggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctagtggaaccggaggagctgagctagtagtacatacatgcagcatcatcgatcgaacgaaatgcatataagcgtttttcaaggttgatctgatgctgcaggtttcgtgattgtataataggaagaaaaagatagtagtattttttatctgagatcatctgtttggaacaggggatttgactaagatttgatataaatttacacaaaatcttagcaaaaatccctttatctcaactctcaagtagagctttgctttgtacaacaaagtatcatgtgtgatataattgtcaggtgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053991.1 RefSeq:Os02g0620600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0620600&diff=183184 2014-06-10T03:38:21Z

<p>LIZHAO: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> # AmmoniAmmonium transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.um transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.&lt;ref name=&quot;ref1&quot; /&gt;<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. Southern blot analysis and screening of the rice genome database confirmed that with OsAMT1;1–1;3the complete AMT1 family of rice had been isolated. &lt;ref name=&quot;ref2&quot; /&gt;<br /> #The amino acid sequences of the OsAMT1;1, OsAMT1;2 and OsAMT1;3 share high sequence similarity to each other and to other AMT1-type ammonium transporters previously identified from other plant species. All three isolated OsAMT1 genes encode functional ammonium transporters.Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3.<br /> #OsAMT1;2 has ammonium transporters activity and mediates ammonium uptake from the soil solution. &lt;ref name=&quot;ref3&quot; /&gt;<br /> <br /> ===Expression===<br /> # OsAMT1;2 is expressed in the central cylinder and cell surface of root tips.<br /> # OsAMT1;2 expression was root specific and ammonium inducible.&lt;ref name=&quot;ref2&quot; /&gt;<br /> # Ammonium showed positive effects on the expression of OsAMT1;2. These effects were mimicked not only by glutamine but also by asparagines. On the other hand,glutamate and aspartate were ineffective with respect to OsAMT1;2.('''Figure 1''').&lt;ref name=&quot;ref3&quot; /&gt;[[File:LIZHAO1.png‎|frame|'''Figure 1'''Hypothetical model of the regulation of OsAMT1 genes in rice roots.]]<br /> OsAMT1gene expression is positively feedback-regulated (dotted arrow) and negatively feedback-regulated (dotted T line) by cytosolic glutamine(Gln). Nitrogen fluxes are shown as solid arrows. See text for details.<br /> <br /> While OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3). while OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3). We complemented a yeast mutant to determine whether the protein encoded by the cDNA is a functional ammonium transporter. Both cDNAs were able to functionally complement the mutant which is deficient in ammonium uptake, indicating that OsAMT1;1 and OsAMT1;2 encodes functional ammonium transporters (data not shown). Thus, OsAMT1;1 and OsAMT1;2 may have different physiological roles in ammonium uptake in rice. There results imply that OsAMT1;1 is involved in uptake and/or salvage of the ammonium in vascular tissues, and OsAMT1;2 in the uptake in root epidermis. Further experiments will be needeed to evaluate this hypothesis.&lt;ref name=&quot;ref4&quot; /&gt;<br /> [[File:LIZHAO2.png‎|frame|'''Figure 2'''Figs. 2 .]]<br /> [[File:LIZHAO3.png‎|frame|'''Figure 2'''Figs. 3 .]]<br /> <br /> ===Evolution===<br /> # Diversity and selective sweep in the OsAMT1;2 genomic region of rice. AMT1;2 transgenic rice plants with enhanced NH4+ permeability show superior growth and higher yield under optimal and suboptimal NH4+ conditions. <br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. ('''Figure4''').&lt;ref name=&quot;ref1&quot; /&gt;<br /> [[File:LIZHAO4.jpg‎|frame|'''Figure4'''Localization of OsAMT1;2mRNA in rice root tip by in situ hybridization.]]<br /> # Hydropathy analysis of the deduced amino acid sequence suggests that OsAMT1;1 and OsAMT1;2 contain 10-11 putative trans-membrane domains. Sequence comparison shows that OsAMT1;1 and OsAMT1;2 are closely related to Arabidopsis and tomato ammonium transporters (67-63%,). ('''Figure5''').&lt;ref name=&quot;ref5&quot; /&gt;<br /> [[File:LIZHAO5.jpg‎|frame|'''Figure5''']]<br /> <br /> ==Labs working on this gene==<br /> # BioScience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan<br /> # State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China)<br /> # Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences,Hunan 410125 (China)<br /> # Department of Botany, the University of British Columbia, Vancouver, BC, Canada <br /> # Agriculture and AgriFood Canada, Shelterbelt Centre Indian Head SK, Canada <br /> # Division of Biology, University of California at San Diego, La Jolla, CA 92093–0116, USA<br /> # Division of Biological Sciences, Graduate School of Science, Hokkaido University, Kita-ku N10-W8, Sapporo, 060-0810 Japan <br /> # CREST, Japan Science and Technology Corporation (JST)CREST, Japan Science and Technology Corporation (JST)<br /> # Division of Bioresources and Product Science, Graduate School of Agriculture, Hokkaido University, Kita-ku N9-W9, Sapporo, 060-8589 Japan <br /> # Plant Science Center, RIKEN, 1-7-22 Suehiro, Tsurumi-ku, Yokohama, 230-0045 Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0620600|<br /> Description = Ammonium transporter family protein|<br /> Version = NM_001053991.1 GI:115447352 GeneID:4330008|<br /> Length = 1853 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0620600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:25548745..25550597|<br /> CDS = 25548816..25550306|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATCADTLGPLLGTAAANATDYLCNQFADTTSAVDSTYLLFSAY LVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGAPSNGFIG KHFFGLKQVPQVGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVY PVVSHWIWSADGWASASRTSGSLLFGSGVIDFAGSGVVHMVGGVAGLWGALIEGPRIG RFDHAGRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTILKSYGPPGSIHGQWSAV GRTAVTTTLAGSTAALTTLFGKRLQTGHWNVIDVCNGLLGGFAAITAGCSVVDPWAAI ICGFVSAWVLIGLNALAARLKFDDPLEAAQLHGGCGAWGVIFTALFARKEYVDQIFGQ PGRPYGLFMGGGGRLLGAHIVVILVIAAWVSFTMAPLFLVLNKLGLLRISAEDEMAGM DQTRHGGFAYAYHDDDASGKPDRSVGGFMLKSAHGTQVAAEMGGHV&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;72..1562#acagcccacacttccattgctcctcccctctcctctacagtctgtgttgagcgcgcgtcgaggcggcgaggatggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctagtggaaccggaggagctgagctagtagtacatacatgcagcatcatcgatcgaacgaaatgcatataagcgtttttcaaggttgatctgatgctgcaggtttcgtgattgtataataggaagaaaaagatagtagtattttttatctgagatcatctgtttggaacaggggatttgactaagatttgatataaatttacacaaaatcttagcaaaaatccctttatctcaactctcaagtagagctttgctttgtacaacaaagtatcatgtgtgatataattgtcaggtgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053991.1 RefSeq:Os02g0620600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0620600&diff=183181 2014-06-10T03:36:18Z

<p>LIZHAO: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> # AmmoniAmmonium transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.um transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.&lt;ref name=&quot;ref1&quot; /&gt;<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. Southern blot analysis and screening of the rice genome database confirmed that with OsAMT1;1–1;3the complete AMT1 family of rice had been isolated. &lt;ref name=&quot;ref2&quot; /&gt;<br /> #The amino acid sequences of the OsAMT1;1, OsAMT1;2 and OsAMT1;3 share high sequence similarity to each other and to other AMT1-type ammonium transporters previously identified from other plant species. All three isolated OsAMT1 genes encode functional ammonium transporters.Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3.<br /> #OsAMT1;2 has ammonium transporters activity and mediates ammonium uptake from the soil solution. &lt;ref name=&quot;ref3&quot; /&gt;<br /> <br /> ===Expression===<br /> # OsAMT1;2 is expressed in the central cylinder and cell surface of root tips.<br /> # OsAMT1;2 expression was root specific and ammonium inducible.&lt;ref name=&quot;ref2&quot; /&gt;<br /> # Ammonium showed positive effects on the expression of OsAMT1;2. These effects were mimicked not only by glutamine but also by asparagines. On the other hand,glutamate and aspartate were ineffective with respect to OsAMT1;2.('''Figure 1''').&lt;ref name=&quot;ref3&quot; /&gt;[[File:LIZHAO1.png‎|frame|'''Figure 1'''Hypothetical model of the regulation of OsAMT1 genes in rice roots.]]<br /> OsAMT1gene expression is positively feedback-regulated (dotted arrow) and negatively feedback-regulated (dotted T line) by cytosolic glutamine(Gln). Nitrogen fluxes are shown as solid arrows. See text for details.<br /> <br /> While OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3). while OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3). We complemented a yeast mutant to determine whether the protein encoded by the cDNA is a functional ammonium transporter. Both cDNAs were able to functionally complement the mutant which is deficient in ammonium uptake, indicating that OsAMT1;1 and OsAMT1;2 encodes functional ammonium transporters (data not shown). Thus, OsAMT1;1 and OsAMT1;2 may have different physiological roles in ammonium uptake in rice. There results imply that OsAMT1;1 is involved in uptake and/or salvage of the ammonium in vascular tissues, and OsAMT1;2 in the uptake in root epidermis. Further experiments will be needeed to evaluate this hypothesis.&lt;ref name=&quot;ref4&quot; /&gt;<br /> [[File:LIZHAO2.png‎|frame|'''Figure 2'''Figs. 2 .]]<br /> [[File:LIZHAO3.png‎|frame|'''Figure 2'''Figs. 3 .]]<br /> <br /> ===Evolution===<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. ('''Figure4''').&lt;ref name=&quot;ref4&quot; /&gt;<br /> [[File:LIZHAO.jpg‎|frame|'''Figure4'''Localization of OsAMT1;2mRNA in rice root tip by in situ hybridization.<br /> # Hydropathy analysis of the deduced amino acid sequence suggests that OsAMT1;1 and OsAMT1;2 contain 10-11 putative trans-membrane domains. Sequence comparison shows that OsAMT1;1 and OsAMT1;2 are closely related to Arabidopsis and tomato ammonium transporters (67-63%,). ('''Figure5''').&lt;ref name=&quot;ref5&quot; /&gt;<br /> [[File:LIZHAO.jpg‎|frame|'''Figure5'''<br /> <br /> ==Labs working on this gene==<br /> # BioScience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan<br /> # State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China)<br /> # Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences,Hunan 410125 (China)<br /> # Department of Botany, the University of British Columbia, Vancouver, BC, Canada <br /> # Agriculture and AgriFood Canada, Shelterbelt Centre Indian Head SK, Canada <br /> # Division of Biology, University of California at San Diego, La Jolla, CA 92093–0116, USA<br /> # Division of Biological Sciences, Graduate School of Science, Hokkaido University, Kita-ku N10-W8, Sapporo, 060-0810 Japan <br /> # CREST, Japan Science and Technology Corporation (JST)CREST, Japan Science and Technology Corporation (JST)<br /> # Division of Bioresources and Product Science, Graduate School of Agriculture, Hokkaido University, Kita-ku N9-W9, Sapporo, 060-8589 Japan <br /> # Plant Science Center, RIKEN, 1-7-22 Suehiro, Tsurumi-ku, Yokohama, 230-0045 Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0620600|<br /> Description = Ammonium transporter family protein|<br /> Version = NM_001053991.1 GI:115447352 GeneID:4330008|<br /> Length = 1853 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0620600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:25548745..25550597|<br /> CDS = 25548816..25550306|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATCADTLGPLLGTAAANATDYLCNQFADTTSAVDSTYLLFSAY LVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGAPSNGFIG KHFFGLKQVPQVGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVY PVVSHWIWSADGWASASRTSGSLLFGSGVIDFAGSGVVHMVGGVAGLWGALIEGPRIG RFDHAGRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTILKSYGPPGSIHGQWSAV GRTAVTTTLAGSTAALTTLFGKRLQTGHWNVIDVCNGLLGGFAAITAGCSVVDPWAAI ICGFVSAWVLIGLNALAARLKFDDPLEAAQLHGGCGAWGVIFTALFARKEYVDQIFGQ PGRPYGLFMGGGGRLLGAHIVVILVIAAWVSFTMAPLFLVLNKLGLLRISAEDEMAGM DQTRHGGFAYAYHDDDASGKPDRSVGGFMLKSAHGTQVAAEMGGHV&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;72..1562#acagcccacacttccattgctcctcccctctcctctacagtctgtgttgagcgcgcgtcgaggcggcgaggatggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctagtggaaccggaggagctgagctagtagtacatacatgcagcatcatcgatcgaacgaaatgcatataagcgtttttcaaggttgatctgatgctgcaggtttcgtgattgtataataggaagaaaaagatagtagtattttttatctgagatcatctgtttggaacaggggatttgactaagatttgatataaatttacacaaaatcttagcaaaaatccctttatctcaactctcaagtagagctttgctttgtacaacaaagtatcatgtgtgatataattgtcaggtgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053991.1 RefSeq:Os02g0620600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:LIZHAO1.png&diff=182803 2014-06-09T15:17:07Z

<p>LIZHAO: Hypothetical model of the regulation of OsAMT1 genes in rice roots.</p> <hr /> <div> Hypothetical model of the regulation of OsAMT1 genes in rice roots.</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0620600&diff=182800 2014-06-09T15:16:16Z

<p>LIZHAO: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> # AmmoniAmmonium transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.um transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.&lt;ref name=&quot;ref1&quot; /&gt;<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. Southern blot analysis and screening of the rice genome database confirmed that with OsAMT1;1–1;3the complete AMT1 family of rice had been isolated. &lt;ref name=&quot;ref2&quot; /&gt;<br /> #The amino acid sequences of the OsAMT1;1, OsAMT1;2 and OsAMT1;3 share high sequence similarity to each other and to other AMT1-type ammonium transporters previously identified from other plant species. All three isolated OsAMT1 genes encode functional ammonium transporters.Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3.<br /> #OsAMT1;2 has ammonium transporters activity and mediates ammonium uptake from the soil solution. &lt;ref name=&quot;ref3&quot; /&gt;<br /> <br /> ===Expression===<br /> # OsAMT1;2 is expressed in the central cylinder and cell surface of root tips.<br /> # OsAMT1;2 expression was root specific and ammonium inducible.<br /> # Ammonium showed positive effects on the expression of OsAMT1;2. These effects were mimicked not only by glutamine but also by asparagines. On the other hand.<br /> # Glutamate and aspartate were ineffective with respect to OsAMT1;2.('''Figure 1''').&lt;ref name=&quot;ref4&quot; /&gt;[[File:LIZHAO1.png‎|frame|'''Figure 1'''Hypothetical model of the regulation of OsAMT1 genes in rice roots.]]<br /> while OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 ).&lt;ref name=&quot;ref2&quot; /&gt;[[File:LIZHAO2.png‎|frame|'''Figure 1'''while OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3).]]<br /> <br /> ===Evolution===<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. ('''Figure4''').&lt;ref name=&quot;ref4&quot; /&gt;<br /> [[File:LIZHAO.jpg‎|frame|'''Figure4'''Localization of OsAMT1;2mRNA in rice root tip by in situ hybridization.<br /> # Hydropathy analysis of the deduced amino acid sequence suggests that OsAMT1;1 and OsAMT1;2 contain 10-11 putative trans-membrane domains. Sequence comparison shows that OsAMT1;1 and OsAMT1;2 are closely related to Arabidopsis and tomato ammonium transporters (67-63%,). ('''Figure5''').&lt;ref name=&quot;ref5&quot; /&gt;<br /> [[File:LIZHAO.jpg‎|frame|'''Figure5'''<br /> <br /> ==Labs working on this gene==<br /> # BioScience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan<br /> # State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China)<br /> # Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences,Hunan 410125 (China)<br /> # Department of Botany, the University of British Columbia, Vancouver, BC, Canada <br /> # Agriculture and AgriFood Canada, Shelterbelt Centre Indian Head SK, Canada <br /> # Division of Biology, University of California at San Diego, La Jolla, CA 92093–0116, USA<br /> # Division of Biological Sciences, Graduate School of Science, Hokkaido University, Kita-ku N10-W8, Sapporo, 060-0810 Japan <br /> # CREST, Japan Science and Technology Corporation (JST)CREST, Japan Science and Technology Corporation (JST)<br /> # Division of Bioresources and Product Science, Graduate School of Agriculture, Hokkaido University, Kita-ku N9-W9, Sapporo, 060-8589 Japan <br /> # Plant Science Center, RIKEN, 1-7-22 Suehiro, Tsurumi-ku, Yokohama, 230-0045 Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0620600|<br /> Description = Ammonium transporter family protein|<br /> Version = NM_001053991.1 GI:115447352 GeneID:4330008|<br /> Length = 1853 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0620600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:25548745..25550597|<br /> CDS = 25548816..25550306|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATCADTLGPLLGTAAANATDYLCNQFADTTSAVDSTYLLFSAY LVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGAPSNGFIG KHFFGLKQVPQVGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVY PVVSHWIWSADGWASASRTSGSLLFGSGVIDFAGSGVVHMVGGVAGLWGALIEGPRIG RFDHAGRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTILKSYGPPGSIHGQWSAV GRTAVTTTLAGSTAALTTLFGKRLQTGHWNVIDVCNGLLGGFAAITAGCSVVDPWAAI ICGFVSAWVLIGLNALAARLKFDDPLEAAQLHGGCGAWGVIFTALFARKEYVDQIFGQ PGRPYGLFMGGGGRLLGAHIVVILVIAAWVSFTMAPLFLVLNKLGLLRISAEDEMAGM DQTRHGGFAYAYHDDDASGKPDRSVGGFMLKSAHGTQVAAEMGGHV&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;72..1562#acagcccacacttccattgctcctcccctctcctctacagtctgtgttgagcgcgcgtcgaggcggcgaggatggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctagtggaaccggaggagctgagctagtagtacatacatgcagcatcatcgatcgaacgaaatgcatataagcgtttttcaaggttgatctgatgctgcaggtttcgtgattgtataataggaagaaaaagatagtagtattttttatctgagatcatctgtttggaacaggggatttgactaagatttgatataaatttacacaaaatcttagcaaaaatccctttatctcaactctcaagtagagctttgctttgtacaacaaagtatcatgtgtgatataattgtcaggtgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053991.1 RefSeq:Os02g0620600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0620600&diff=182798 2014-06-09T15:15:23Z

<p>LIZHAO: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> # AmmoniAmmonium transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.um transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.&lt;ref name=&quot;ref1&quot; /&gt;<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. Southern blot analysis and screening of the rice genome database confirmed that with OsAMT1;1–1;3the complete AMT1 family of rice had been isolated. &lt;ref name=&quot;ref2&quot; /&gt;<br /> #The amino acid sequences of the OsAMT1;1, OsAMT1;2 and OsAMT1;3 share high sequence similarity to each other and to other AMT1-type ammonium transporters previously identified from other plant species. All three isolated OsAMT1 genes encode functional ammonium transporters.Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3.<br /> #OsAMT1;2 has ammonium transporters activity and mediates ammonium uptake from the soil solution. &lt;ref name=&quot;ref3&quot; /&gt;<br /> <br /> ===Expression===<br /> # OsAMT1;2 is expressed in the central cylinder and cell surface of root tips.<br /> # OsAMT1;2 expression was root specific and ammonium inducible.<br /> # Ammonium showed positive effects on the expression of OsAMT1;2. These effects were mimicked not only by glutamine but also by asparagines. On the other hand.<br /> # Glutamate and aspartate were ineffective with respect to OsAMT1;2.('''Figure 1''').&lt;ref name=&quot;ref4&quot; /&gt;[[File:LIZHAO.png‎|frame|'''Figure 1'''Hypothetical model of the regulation of OsAMT1 genes in rice roots.]]<br /> while OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 ).&lt;ref name=&quot;ref2&quot; /&gt;[[File:LIZHAO.png‎|frame|'''Figure 1'''while OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3).]]<br /> <br /> ===Evolution===<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. ('''Figure4''').&lt;ref name=&quot;ref4&quot; /&gt;<br /> [[File:LIZHAO.jpg‎|frame|'''Figure4'''Localization of OsAMT1;2mRNA in rice root tip by in situ hybridization.<br /> # Hydropathy analysis of the deduced amino acid sequence suggests that OsAMT1;1 and OsAMT1;2 contain 10-11 putative trans-membrane domains. Sequence comparison shows that OsAMT1;1 and OsAMT1;2 are closely related to Arabidopsis and tomato ammonium transporters (67-63%,). ('''Figure5''').&lt;ref name=&quot;ref5&quot; /&gt;<br /> [[File:LIZHAO.jpg‎|frame|'''Figure5'''<br /> <br /> ==Labs working on this gene==<br /> # BioScience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan<br /> # State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China)<br /> # Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences,Hunan 410125 (China)<br /> # Department of Botany, the University of British Columbia, Vancouver, BC, Canada <br /> # Agriculture and AgriFood Canada, Shelterbelt Centre Indian Head SK, Canada <br /> # Division of Biology, University of California at San Diego, La Jolla, CA 92093–0116, USA<br /> # Division of Biological Sciences, Graduate School of Science, Hokkaido University, Kita-ku N10-W8, Sapporo, 060-0810 Japan <br /> # CREST, Japan Science and Technology Corporation (JST)CREST, Japan Science and Technology Corporation (JST)<br /> # Division of Bioresources and Product Science, Graduate School of Agriculture, Hokkaido University, Kita-ku N9-W9, Sapporo, 060-8589 Japan <br /> # Plant Science Center, RIKEN, 1-7-22 Suehiro, Tsurumi-ku, Yokohama, 230-0045 Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0620600|<br /> Description = Ammonium transporter family protein|<br /> Version = NM_001053991.1 GI:115447352 GeneID:4330008|<br /> Length = 1853 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0620600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:25548745..25550597|<br /> CDS = 25548816..25550306|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATCADTLGPLLGTAAANATDYLCNQFADTTSAVDSTYLLFSAY LVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGAPSNGFIG KHFFGLKQVPQVGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVY PVVSHWIWSADGWASASRTSGSLLFGSGVIDFAGSGVVHMVGGVAGLWGALIEGPRIG RFDHAGRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTILKSYGPPGSIHGQWSAV GRTAVTTTLAGSTAALTTLFGKRLQTGHWNVIDVCNGLLGGFAAITAGCSVVDPWAAI ICGFVSAWVLIGLNALAARLKFDDPLEAAQLHGGCGAWGVIFTALFARKEYVDQIFGQ PGRPYGLFMGGGGRLLGAHIVVILVIAAWVSFTMAPLFLVLNKLGLLRISAEDEMAGM DQTRHGGFAYAYHDDDASGKPDRSVGGFMLKSAHGTQVAAEMGGHV&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;72..1562#acagcccacacttccattgctcctcccctctcctctacagtctgtgttgagcgcgcgtcgaggcggcgaggatggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctagtggaaccggaggagctgagctagtagtacatacatgcagcatcatcgatcgaacgaaatgcatataagcgtttttcaaggttgatctgatgctgcaggtttcgtgattgtataataggaagaaaaagatagtagtattttttatctgagatcatctgtttggaacaggggatttgactaagatttgatataaatttacacaaaatcttagcaaaaatccctttatctcaactctcaagtagagctttgctttgtacaacaaagtatcatgtgtgatataattgtcaggtgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053991.1 RefSeq:Os02g0620600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:LIZHAO.png&diff=182788 2014-06-09T15:12:21Z

<p>LIZHAO: uploaded a new version of &amp;quot;File:LIZHAO.png&amp;quot;: OsAMT1gene expression is positively feedback-regulated (dotted arrow) and negatively feedback-regulated (dotted T line) by cytosolic glutamine(Gln). Nitrogen fluxes are shown as solid arrows. See </p> <hr /> <div>Hypothetical model of the regulation of OsAMT1 genes in rice roots.</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0620600&diff=182783 2014-06-09T15:10:21Z

<p>LIZHAO: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> # AmmoniAmmonium transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.um transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.&lt;ref name=&quot;ref1&quot; /&gt;<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. Southern blot analysis and screening of the rice genome database confirmed that with OsAMT1;1–1;3the complete AMT1 family of rice had been isolated. &lt;ref name=&quot;ref2&quot; /&gt;<br /> #The amino acid sequences of the OsAMT1;1, OsAMT1;2 and OsAMT1;3 share high sequence similarity to each other and to other AMT1-type ammonium transporters previously identified from other plant species. All three isolated OsAMT1 genes encode functional ammonium transporters.Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3.<br /> #OsAMT1;2 has ammonium transporters activity and mediates ammonium uptake from the soil solution. &lt;ref name=&quot;ref3&quot; /&gt;<br /> <br /> ===Expression===<br /> # OsAMT1;2 is expressed in the central cylinder and cell surface of root tips.<br /> # OsAMT1;2 expression was root specific and ammonium inducible.<br /> # Ammonium showed positive effects on the expression of OsAMT1;2. These effects were mimicked not only by glutamine but also by asparagines. On the other hand.<br /> # Glutamate and aspartate were ineffective with respect to OsAMT1;2.('''Figure 1''').&lt;ref name=&quot;ref4&quot; /&gt;[[File:LIZHAO.png‎|frame|'''Figure 1'''Hypothetical model of the regulation of OsAMT1 genes in rice roots.]]<br /> while OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3).&lt;ref name=&quot;ref2&quot; /&gt;<br /> <br /> ===Evolution===<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. ('''Figure4''').&lt;ref name=&quot;ref4&quot; /&gt;<br /> [[File:LIZHAO.jpg‎|frame|'''Figure4'''Localization of OsAMT1;2mRNA in rice root tip by in situ hybridization.<br /> # Hydropathy analysis of the deduced amino acid sequence suggests that OsAMT1;1 and OsAMT1;2 contain 10-11 putative trans-membrane domains. Sequence comparison shows that OsAMT1;1 and OsAMT1;2 are closely related to Arabidopsis and tomato ammonium transporters (67-63%,). ('''Figure5''').&lt;ref name=&quot;ref5&quot; /&gt;<br /> [[File:LIZHAO.jpg‎|frame|'''Figure5'''<br /> <br /> ==Labs working on this gene==<br /> # BioScience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan<br /> # State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China)<br /> # Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences,Hunan 410125 (China)<br /> # Department of Botany, the University of British Columbia, Vancouver, BC, Canada <br /> # Agriculture and AgriFood Canada, Shelterbelt Centre Indian Head SK, Canada <br /> # Division of Biology, University of California at San Diego, La Jolla, CA 92093–0116, USA<br /> # Division of Biological Sciences, Graduate School of Science, Hokkaido University, Kita-ku N10-W8, Sapporo, 060-0810 Japan <br /> # CREST, Japan Science and Technology Corporation (JST)CREST, Japan Science and Technology Corporation (JST)<br /> # Division of Bioresources and Product Science, Graduate School of Agriculture, Hokkaido University, Kita-ku N9-W9, Sapporo, 060-8589 Japan <br /> # Plant Science Center, RIKEN, 1-7-22 Suehiro, Tsurumi-ku, Yokohama, 230-0045 Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0620600|<br /> Description = Ammonium transporter family protein|<br /> Version = NM_001053991.1 GI:115447352 GeneID:4330008|<br /> Length = 1853 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0620600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:25548745..25550597|<br /> CDS = 25548816..25550306|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATCADTLGPLLGTAAANATDYLCNQFADTTSAVDSTYLLFSAY LVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGAPSNGFIG KHFFGLKQVPQVGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVY PVVSHWIWSADGWASASRTSGSLLFGSGVIDFAGSGVVHMVGGVAGLWGALIEGPRIG RFDHAGRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTILKSYGPPGSIHGQWSAV GRTAVTTTLAGSTAALTTLFGKRLQTGHWNVIDVCNGLLGGFAAITAGCSVVDPWAAI ICGFVSAWVLIGLNALAARLKFDDPLEAAQLHGGCGAWGVIFTALFARKEYVDQIFGQ PGRPYGLFMGGGGRLLGAHIVVILVIAAWVSFTMAPLFLVLNKLGLLRISAEDEMAGM DQTRHGGFAYAYHDDDASGKPDRSVGGFMLKSAHGTQVAAEMGGHV&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;72..1562#acagcccacacttccattgctcctcccctctcctctacagtctgtgttgagcgcgcgtcgaggcggcgaggatggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctagtggaaccggaggagctgagctagtagtacatacatgcagcatcatcgatcgaacgaaatgcatataagcgtttttcaaggttgatctgatgctgcaggtttcgtgattgtataataggaagaaaaagatagtagtattttttatctgagatcatctgtttggaacaggggatttgactaagatttgatataaatttacacaaaatcttagcaaaaatccctttatctcaactctcaagtagagctttgctttgtacaacaaagtatcatgtgtgatataattgtcaggtgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053991.1 RefSeq:Os02g0620600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0620600&diff=182777 2014-06-09T15:08:42Z

<p>LIZHAO: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> # AmmoniAmmonium transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.um transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.&lt;ref name=&quot;ref1&quot; /&gt;<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. Southern blot analysis and screening of the rice genome database confirmed that with OsAMT1;1–1;3the complete AMT1 family of rice had been isolated. &lt;ref name=&quot;ref2&quot; /&gt;<br /> #The amino acid sequences of the OsAMT1;1, OsAMT1;2 and OsAMT1;3 share high sequence similarity to each other and to other AMT1-type ammonium transporters previously identified from other plant species. All three isolated OsAMT1 genes encode functional ammonium transporters.Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3.<br /> #OsAMT1;2 has ammonium transporters activity and mediates ammonium uptake from the soil solution. &lt;ref name=&quot;ref3&quot; /&gt;<br /> <br /> ===Expression===<br /> # OsAMT1;2 is expressed in the central cylinder and cell surface of root tips.<br /> # OsAMT1;2 expression was root specific and ammonium inducible.<br /> # Ammonium showed positive effects on the expression of OsAMT1;2. These effects were mimicked not only by glutamine but also by asparagines. On the other hand.<br /> # Glutamate and aspartate were ineffective with respect to OsAMT1;2.('''Figure 1''').&lt;ref name=&quot;ref4&quot; /&gt;<br /> [[File:LIZHAO.png‎|frame|'''Figure 1'''Hypothetical model of the regulation of OsAMT1 genes in rice roots. <br /> while OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3).&lt;ref name=&quot;ref2&quot; /&gt;<br /> <br /> ===Evolution===<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. ('''Figure4''').&lt;ref name=&quot;ref4&quot; /&gt;<br /> [[File:LIZHAO.jpg‎|frame|'''Figure4'''Localization of OsAMT1;2mRNA in rice root tip by in situ hybridization.<br /> # Hydropathy analysis of the deduced amino acid sequence suggests that OsAMT1;1 and OsAMT1;2 contain 10-11 putative trans-membrane domains. Sequence comparison shows that OsAMT1;1 and OsAMT1;2 are closely related to Arabidopsis and tomato ammonium transporters (67-63%,). ('''Figure5''').&lt;ref name=&quot;ref5&quot; /&gt;<br /> [[File:LIZHAO.jpg‎|frame|'''Figure5'''<br /> <br /> ==Labs working on this gene==<br /> # BioScience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan<br /> # State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China)<br /> # Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences,Hunan 410125 (China)<br /> # Department of Botany, the University of British Columbia, Vancouver, BC, Canada <br /> # Agriculture and AgriFood Canada, Shelterbelt Centre Indian Head SK, Canada <br /> # Division of Biology, University of California at San Diego, La Jolla, CA 92093–0116, USA<br /> # Division of Biological Sciences, Graduate School of Science, Hokkaido University, Kita-ku N10-W8, Sapporo, 060-0810 Japan <br /> # CREST, Japan Science and Technology Corporation (JST)CREST, Japan Science and Technology Corporation (JST)<br /> # Division of Bioresources and Product Science, Graduate School of Agriculture, Hokkaido University, Kita-ku N9-W9, Sapporo, 060-8589 Japan <br /> # Plant Science Center, RIKEN, 1-7-22 Suehiro, Tsurumi-ku, Yokohama, 230-0045 Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0620600|<br /> Description = Ammonium transporter family protein|<br /> Version = NM_001053991.1 GI:115447352 GeneID:4330008|<br /> Length = 1853 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0620600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:25548745..25550597|<br /> CDS = 25548816..25550306|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATCADTLGPLLGTAAANATDYLCNQFADTTSAVDSTYLLFSAY LVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGAPSNGFIG KHFFGLKQVPQVGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVY PVVSHWIWSADGWASASRTSGSLLFGSGVIDFAGSGVVHMVGGVAGLWGALIEGPRIG RFDHAGRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTILKSYGPPGSIHGQWSAV GRTAVTTTLAGSTAALTTLFGKRLQTGHWNVIDVCNGLLGGFAAITAGCSVVDPWAAI ICGFVSAWVLIGLNALAARLKFDDPLEAAQLHGGCGAWGVIFTALFARKEYVDQIFGQ PGRPYGLFMGGGGRLLGAHIVVILVIAAWVSFTMAPLFLVLNKLGLLRISAEDEMAGM DQTRHGGFAYAYHDDDASGKPDRSVGGFMLKSAHGTQVAAEMGGHV&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;72..1562#acagcccacacttccattgctcctcccctctcctctacagtctgtgttgagcgcgcgtcgaggcggcgaggatggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctagtggaaccggaggagctgagctagtagtacatacatgcagcatcatcgatcgaacgaaatgcatataagcgtttttcaaggttgatctgatgctgcaggtttcgtgattgtataataggaagaaaaagatagtagtattttttatctgagatcatctgtttggaacaggggatttgactaagatttgatataaatttacacaaaatcttagcaaaaatccctttatctcaactctcaagtagagctttgctttgtacaacaaagtatcatgtgtgatataattgtcaggtgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053991.1 RefSeq:Os02g0620600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:LIZHAO.png&diff=182773 2014-06-09T15:06:54Z

<p>LIZHAO: Hypothetical model of the regulation of OsAMT1 genes in rice roots.</p> <hr /> <div>Hypothetical model of the regulation of OsAMT1 genes in rice roots.</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0620600&diff=182739 2014-06-09T14:57:24Z

<p>LIZHAO: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> # AmmoniAmmonium transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.um transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.&lt;ref name=&quot;ref1&quot; /&gt;<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. Southern blot analysis and screening of the rice genome database confirmed that with OsAMT1;1–1;3the complete AMT1 family of rice had been isolated. &lt;ref name=&quot;ref2&quot; /&gt;<br /> #The amino acid sequences of the OsAMT1;1, OsAMT1;2 and OsAMT1;3 share high sequence similarity to each other and to other AMT1-type ammonium transporters previously identified from other plant species. All three isolated OsAMT1 genes encode functional ammonium transporters.Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3.<br /> #OsAMT1;2 has ammonium transporters activity and mediates ammonium uptake from the soil solution. &lt;ref name=&quot;ref3&quot; /&gt;<br /> <br /> ===Expression===<br /> # OsAMT1;2 is expressed in the central cylinder and cell surface of root tips.<br /> # OsAMT1;2 expression was root specific and ammonium inducible.<br /> # Ammonium showed positive effects on the expression of OsAMT1;2. These effects were mimicked not only by glutamine but also by asparagines. On the other hand.<br /> # Glutamate and aspartate were ineffective with respect to OsAMT1;2.('''Figure 1''').&lt;ref name=&quot;ref4&quot; /&gt;<br /> [[File:LIZHAO.jpg‎|frame|'''Figure 1'''Hypothetical model of the regulation of OsAMT1 genes in rice roots. <br /> while OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3).&lt;ref name=&quot;ref2&quot; /&gt;<br /> <br /> ===Evolution===<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. ('''Figure4''').&lt;ref name=&quot;ref4&quot; /&gt;<br /> [[File:LIZHAO.jpg‎|frame|'''Figure4'''Localization of OsAMT1;2mRNA in rice root tip by in situ hybridization.<br /> # Hydropathy analysis of the deduced amino acid sequence suggests that OsAMT1;1 and OsAMT1;2 contain 10-11 putative trans-membrane domains. Sequence comparison shows that OsAMT1;1 and OsAMT1;2 are closely related to Arabidopsis and tomato ammonium transporters (67-63%,). ('''Figure5''').&lt;ref name=&quot;ref5&quot; /&gt;<br /> [[File:LIZHAO.jpg‎|frame|'''Figure5'''<br /> <br /> ==Labs working on this gene==<br /> # BioScience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan<br /> # State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China)<br /> # Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences,Hunan 410125 (China)<br /> # Department of Botany, the University of British Columbia, Vancouver, BC, Canada <br /> # Agriculture and AgriFood Canada, Shelterbelt Centre Indian Head SK, Canada <br /> # Division of Biology, University of California at San Diego, La Jolla, CA 92093–0116, USA<br /> # Division of Biological Sciences, Graduate School of Science, Hokkaido University, Kita-ku N10-W8, Sapporo, 060-0810 Japan <br /> # CREST, Japan Science and Technology Corporation (JST)CREST, Japan Science and Technology Corporation (JST)<br /> # Division of Bioresources and Product Science, Graduate School of Agriculture, Hokkaido University, Kita-ku N9-W9, Sapporo, 060-8589 Japan <br /> # Plant Science Center, RIKEN, 1-7-22 Suehiro, Tsurumi-ku, Yokohama, 230-0045 Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0620600|<br /> Description = Ammonium transporter family protein|<br /> Version = NM_001053991.1 GI:115447352 GeneID:4330008|<br /> Length = 1853 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0620600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:25548745..25550597|<br /> CDS = 25548816..25550306|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATCADTLGPLLGTAAANATDYLCNQFADTTSAVDSTYLLFSAY LVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGAPSNGFIG KHFFGLKQVPQVGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVY PVVSHWIWSADGWASASRTSGSLLFGSGVIDFAGSGVVHMVGGVAGLWGALIEGPRIG RFDHAGRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTILKSYGPPGSIHGQWSAV GRTAVTTTLAGSTAALTTLFGKRLQTGHWNVIDVCNGLLGGFAAITAGCSVVDPWAAI ICGFVSAWVLIGLNALAARLKFDDPLEAAQLHGGCGAWGVIFTALFARKEYVDQIFGQ PGRPYGLFMGGGGRLLGAHIVVILVIAAWVSFTMAPLFLVLNKLGLLRISAEDEMAGM DQTRHGGFAYAYHDDDASGKPDRSVGGFMLKSAHGTQVAAEMGGHV&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;72..1562#acagcccacacttccattgctcctcccctctcctctacagtctgtgttgagcgcgcgtcgaggcggcgaggatggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctagtggaaccggaggagctgagctagtagtacatacatgcagcatcatcgatcgaacgaaatgcatataagcgtttttcaaggttgatctgatgctgcaggtttcgtgattgtataataggaagaaaaagatagtagtattttttatctgagatcatctgtttggaacaggggatttgactaagatttgatataaatttacacaaaatcttagcaaaaatccctttatctcaactctcaagtagagctttgctttgtacaacaaagtatcatgtgtgatataattgtcaggtgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053991.1 RefSeq:Os02g0620600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0620600&diff=182709 2014-06-09T14:35:24Z

<p>LIZHAO: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> # AmmoniAmmonium transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.um transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.&lt;ref name=&quot;ref1&quot; /&gt;<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. Southern blot analysis and screening of the rice genome database confirmed that with OsAMT1;1–1;3the complete AMT1 family of rice had been isolated. &lt;ref name=&quot;ref2&quot; /&gt;<br /> #The amino acid sequences of the OsAMT1;1, OsAMT1;2 and OsAMT1;3 share high sequence similarity to each other and to other AMT1-type ammonium transporters previously identified from other plant species. All three isolated OsAMT1 genes encode functional ammonium transporters.Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3.<br /> #OsAMT1;2 has ammonium transporters activity and mediates ammonium uptake from the soil solution. &lt;ref name=&quot;ref3&quot; /&gt;<br /> <br /> ===Expression===<br /> # OsAMT1;2 is expressed in the central cylinder and cell surface of root tips.<br /> # OsAMT1;2 expression was root specific and ammonium inducible.<br /> # Ammonium showed positive effects on the expression of OsAMT1;2. These effects were mimicked not only by glutamine but also by asparagines. On the other hand.<br /> # Glutamate and aspartate were ineffective with respect to OsAMT1;2.('''Figure 1''').&lt;ref name=&quot;ref4&quot; /&gt;<br /> [[File:LIZHAO.jpg‎|frame|'''Figure 1'''Hypothetical model of the regulation of OsAMT1 genes in rice roots. <br /> while OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3).&lt;ref name=&quot;ref2&quot; /&gt;<br /> <br /> ===Evolution===<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. ('''Figure4''').&lt;ref name=&quot;ref4&quot; /&gt;<br /> [[File:LIZHAO.jpg‎|frame|'''Figure4'''Localization of OsAMT1;2mRNA in rice root tip by in situ hybridization.<br /> # Hydropathy analysis of the deduced amino acid sequence suggests that OsAMT1;1 and OsAMT1;2 contain 10-11 putative trans-membrane domains. Sequence comparison shows that OsAMT1;1 and OsAMT1;2 are closely related to Arabidopsis and tomato ammonium transporters (67-63%,). ('''Figure5''').&lt;ref name=&quot;ref5&quot; /&gt;<br /> [[File:LIZHAO.jpg‎|frame|'''Figure5'''<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0620600|<br /> Description = Ammonium transporter family protein|<br /> Version = NM_001053991.1 GI:115447352 GeneID:4330008|<br /> Length = 1853 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0620600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:25548745..25550597|<br /> CDS = 25548816..25550306|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATCADTLGPLLGTAAANATDYLCNQFADTTSAVDSTYLLFSAY LVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGAPSNGFIG KHFFGLKQVPQVGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVY PVVSHWIWSADGWASASRTSGSLLFGSGVIDFAGSGVVHMVGGVAGLWGALIEGPRIG RFDHAGRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTILKSYGPPGSIHGQWSAV GRTAVTTTLAGSTAALTTLFGKRLQTGHWNVIDVCNGLLGGFAAITAGCSVVDPWAAI ICGFVSAWVLIGLNALAARLKFDDPLEAAQLHGGCGAWGVIFTALFARKEYVDQIFGQ PGRPYGLFMGGGGRLLGAHIVVILVIAAWVSFTMAPLFLVLNKLGLLRISAEDEMAGM DQTRHGGFAYAYHDDDASGKPDRSVGGFMLKSAHGTQVAAEMGGHV&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;72..1562#acagcccacacttccattgctcctcccctctcctctacagtctgtgttgagcgcgcgtcgaggcggcgaggatggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctagtggaaccggaggagctgagctagtagtacatacatgcagcatcatcgatcgaacgaaatgcatataagcgtttttcaaggttgatctgatgctgcaggtttcgtgattgtataataggaagaaaaagatagtagtattttttatctgagatcatctgtttggaacaggggatttgactaagatttgatataaatttacacaaaatcttagcaaaaatccctttatctcaactctcaagtagagctttgctttgtacaacaaagtatcatgtgtgatataattgtcaggtgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053991.1 RefSeq:Os02g0620600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0620600&diff=182692 2014-06-09T14:28:02Z

<p>LIZHAO: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> # AmmoniAmmonium transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.um transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.&lt;ref name=&quot;ref1&quot; /&gt;<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. Southern blot analysis and screening of the rice genome database confirmed that with OsAMT1;1–1;3the complete AMT1 family of rice had been isolated. &lt;ref name=&quot;ref2&quot; /&gt;<br /> #The amino acid sequences of the OsAMT1;1, OsAMT1;2 and OsAMT1;3 share high sequence similarity to each other and to other AMT1-type ammonium transporters previously identified from other plant species. All three isolated OsAMT1 genes encode functional ammonium transporters.Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3.<br /> #OsAMT1;2 has ammonium transporters activity and mediates ammonium uptake from the soil solution. &lt;ref name=&quot;ref3&quot; /&gt;<br /> <br /> ===Expression===<br /> # OsAMT1;2 is expressed in the central cylinder and cell surface of root tips.<br /> # OsAMT1;2 expression was root specific and ammonium inducible.<br /> # Ammonium showed positive effects on the expression of OsAMT1;2. These effects were mimicked not only by glutamine but also by asparagines. On the other hand.<br /> # Glutamate and aspartate were ineffective with respect to OsAMT1;2.('''Figure 1''').&lt;ref name=&quot;ref4&quot; /&gt;<br /> [[File:LIZHAO.jpg‎|frame|'''Figure 1'''Hypothetical model of the regulation of OsAMT1 genes in rice roots. <br /> while OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3).&lt;ref name=&quot;ref2&quot; /&gt;<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0620600|<br /> Description = Ammonium transporter family protein|<br /> Version = NM_001053991.1 GI:115447352 GeneID:4330008|<br /> Length = 1853 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0620600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:25548745..25550597|<br /> CDS = 25548816..25550306|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATCADTLGPLLGTAAANATDYLCNQFADTTSAVDSTYLLFSAY LVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGAPSNGFIG KHFFGLKQVPQVGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVY PVVSHWIWSADGWASASRTSGSLLFGSGVIDFAGSGVVHMVGGVAGLWGALIEGPRIG RFDHAGRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTILKSYGPPGSIHGQWSAV GRTAVTTTLAGSTAALTTLFGKRLQTGHWNVIDVCNGLLGGFAAITAGCSVVDPWAAI ICGFVSAWVLIGLNALAARLKFDDPLEAAQLHGGCGAWGVIFTALFARKEYVDQIFGQ PGRPYGLFMGGGGRLLGAHIVVILVIAAWVSFTMAPLFLVLNKLGLLRISAEDEMAGM DQTRHGGFAYAYHDDDASGKPDRSVGGFMLKSAHGTQVAAEMGGHV&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;72..1562#acagcccacacttccattgctcctcccctctcctctacagtctgtgttgagcgcgcgtcgaggcggcgaggatggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctagtggaaccggaggagctgagctagtagtacatacatgcagcatcatcgatcgaacgaaatgcatataagcgtttttcaaggttgatctgatgctgcaggtttcgtgattgtataataggaagaaaaagatagtagtattttttatctgagatcatctgtttggaacaggggatttgactaagatttgatataaatttacacaaaatcttagcaaaaatccctttatctcaactctcaagtagagctttgctttgtacaacaaagtatcatgtgtgatataattgtcaggtgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053991.1 RefSeq:Os02g0620600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0620600&diff=182688 2014-06-09T14:22:37Z

<p>LIZHAO: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> # AmmoniAmmonium transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.um transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.&lt;ref name=&quot;ref1&quot; /&gt;<br /> # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. Southern blot analysis and screening of the rice genome database confirmed that with OsAMT1;1–1;3the complete AMT1 family of rice had been isolated. &lt;ref name=&quot;ref2&quot; /&gt;<br /> #The amino acid sequences of the OsAMT1;1, OsAMT1;2 and OsAMT1;3 share high sequence similarity to each other and to other AMT1-type ammonium transporters previously identified from other plant species. All three isolated OsAMT1 genes encode functional ammonium transporters.Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3.<br /> #OsAMT1;2 has ammonium transporters activity and mediates ammonium uptake from the soil solution. &lt;ref name=&quot;ref3&quot; /&gt;<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0620600|<br /> Description = Ammonium transporter family protein|<br /> Version = NM_001053991.1 GI:115447352 GeneID:4330008|<br /> Length = 1853 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0620600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:25548745..25550597|<br /> CDS = 25548816..25550306|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATCADTLGPLLGTAAANATDYLCNQFADTTSAVDSTYLLFSAY LVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGAPSNGFIG KHFFGLKQVPQVGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVY PVVSHWIWSADGWASASRTSGSLLFGSGVIDFAGSGVVHMVGGVAGLWGALIEGPRIG RFDHAGRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTILKSYGPPGSIHGQWSAV GRTAVTTTLAGSTAALTTLFGKRLQTGHWNVIDVCNGLLGGFAAITAGCSVVDPWAAI ICGFVSAWVLIGLNALAARLKFDDPLEAAQLHGGCGAWGVIFTALFARKEYVDQIFGQ PGRPYGLFMGGGGRLLGAHIVVILVIAAWVSFTMAPLFLVLNKLGLLRISAEDEMAGM DQTRHGGFAYAYHDDDASGKPDRSVGGFMLKSAHGTQVAAEMGGHV&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;72..1562#acagcccacacttccattgctcctcccctctcctctacagtctgtgttgagcgcgcgtcgaggcggcgaggatggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctagtggaaccggaggagctgagctagtagtacatacatgcagcatcatcgatcgaacgaaatgcatataagcgtttttcaaggttgatctgatgctgcaggtttcgtgattgtataataggaagaaaaagatagtagtattttttatctgagatcatctgtttggaacaggggatttgactaagatttgatataaatttacacaaaatcttagcaaaaatccctttatctcaactctcaagtagagctttgctttgtacaacaaagtatcatgtgtgatataattgtcaggtgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053991.1 RefSeq:Os02g0620600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0620600&diff=182682 2014-06-09T14:15:53Z

<p>LIZHAO: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> Ammonium transporter probably involved in ammonium uptake from the soil and ammonium uptake and retrieval in the vascular system.<br /> OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. Southern blot analysis and screening of the rice genome database confirmed that with OsAMT1;1–1;3the complete AMT1 family of rice had been isolated. <br /> The amino acid sequences of the OsAMT1;1, OsAMT1;2 and OsAMT1;3 share high sequence similarity to each other and to other AMT1-type ammonium transporters previously identified from other plant species. All three isolated OsAMT1 genes encode functional ammonium transporters.<br /> Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3.<br /> OsAMT1;2 has ammonium transporters activity and mediates ammonium uptake from the soil solution.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os02g0620600|<br /> Description = Ammonium transporter family protein|<br /> Version = NM_001053991.1 GI:115447352 GeneID:4330008|<br /> Length = 1853 bp|<br /> Definition = Oryza sativa Japonica Group Os02g0620600, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|<br /> AP = Chromosome 2:25548745..25550597|<br /> CDS = 25548816..25550306|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008395:25548745..25550597<br /> source=RiceChromosome02<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MATCADTLGPLLGTAAANATDYLCNQFADTTSAVDSTYLLFSAY LVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGAPSNGFIG KHFFGLKQVPQVGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVY PVVSHWIWSADGWASASRTSGSLLFGSGVIDFAGSGVVHMVGGVAGLWGALIEGPRIG RFDHAGRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTILKSYGPPGSIHGQWSAV GRTAVTTTLAGSTAALTTLFGKRLQTGHWNVIDVCNGLLGGFAAITAGCSVVDPWAAI ICGFVSAWVLIGLNALAARLKFDDPLEAAQLHGGCGAWGVIFTALFARKEYVDQIFGQ PGRPYGLFMGGGGRLLGAHIVVILVIAAWVSFTMAPLFLVLNKLGLLRISAEDEMAGM DQTRHGGFAYAYHDDDASGKPDRSVGGFMLKSAHGTQVAAEMGGHV&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;72..1562#acagcccacacttccattgctcctcccctctcctctacagtctgtgttgagcgcgcgtcgaggcggcgaggatggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctagtggaaccggaggagctgagctagtagtacatacatgcagcatcatcgatcgaacgaaatgcatataagcgtttttcaaggttgatctgatgctgcaggtttcgtgattgtataataggaagaaaaagatagtagtattttttatctgagatcatctgtttggaacaggggatttgactaagatttgatataaatttacacaaaatcttagcaaaaatccctttatctcaactctcaagtagagctttgctttgtacaacaaagtatcatgtgtgatataattgtcaggtgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053991.1 RefSeq:Os02g0620600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 2]]<br /> [[Category:Chromosome 2]]</div>

LIZHAO https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0203800&diff=181330 2014-06-08T14:08:35Z

<p>LIZHAO: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information <br /> just for log<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os06g0203800|<br /> Description = Similar to ERECTA-like kinase 1|<br /> Version = NM_001063622.1 GI:115466975 GeneID:4340427|<br /> Length = 7605 bp|<br /> Definition = Oryza sativa Japonica Group Os06g0203800, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br /> AP = Chromosome 6:5243240..5250844|<br /> CDS = 5243606..5243974,5244471..5244844,5245029..5245376,5245553..5245681,5245769..5245842&lt;br&gt;,5245929..5246000,5246085..5246153,5246243..5246314,5246392..5246463&lt;br&gt;,5246544..5246615,5246696..5246767,5246919..5246990,5247071..5247142&lt;br&gt;,5247234..5247305,5247387..5247458,5247611..5247682,5247783..5247854&lt;br&gt;,5247948..5248016,5248102..5248173,5248256..5248327,5248612..5248683&lt;br&gt;,5248763..5248834,5248946..5249017,5249103..5249174,5249274..5249345&lt;br&gt;,5249433..5249562,5250396..5250474|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008399:5243240..5250844<br /> source=RiceChromosome06<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008399:5243240..5250844<br /> source=RiceChromosome06<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgacgccggcgccggcggccgcctcctaccgcgctctcgtcgcgctcctgctcgtcgccgtcgccgttgccgatgatgggtcgacgctgctggagatcaagaagtccttccgcaatgtggacaacgtactgtacgattgggccggcggcgactactgctcgtggcgcggcgtcctctgcgacaacgtcaccttcgccgtcgccgcgctcaacctatccgggctcaacctcggaggcgagatctctccggccgtcggcaggttgaagggcatcgtctcgattgacttgaagtcgaatgggctgtctgggcagatccctgatgagattggcgattgttcatcactaaaaactctggatttgtctttcaatagcttggatggggacattccgttctcagtatcgaagctgaagcacattgagagcttgatattgaagaacaaccaactgatcggagtgatcccatcaacgctctcacagctcccaaatttgaagattttggacttggcacagaacaaactgagtggagagataccaagactgatatattggaacgaggttcttcaatacttgggattacgcggtaataatttagaaggcagcatctccccagatatatgccagttgactgggctttggtactttgacgtaaagaacaacagcttgactgggccgataccagaaaccattgggaactgtacaagttttcaggtcttggatttgtcttacaataaactttctggatcaattcctttcaacattggtttcctacaagttgctacactatctttgcaagggaacatgtttactggtcctattccatcagttattggacttatgcaggctctcgctgtactggatctgagttacaaccaattgtctggtcctattccatcgatactaggcaatttaacatacactgagaagctgtatatgcaaggcaataagttaacaggtccaataccacctgagcttggaaatatgtcaacccttcattacttagaacttaacgataatcaacttagcgggttcattcctccagagttcggaaagctaacagggttatttgacttaaaccttgcaaacaacaactttgaaggtccaatccctgataacataagctcatgtgtgaatctcaatagcttcaatgcttatggcaacagattaaatgggaccattcctccttcattgcataaacttgagagcatgacttatttgaatttgtcatcaaattttctaagtggttctattcctattgagctatcgagaatcaacaatttggacaccttggatttatcctgtaacatgattactggcccaattccatcaaccattgggagtttggagcatctattaagacttaacttgagcaacaatggtctagtaggattcattcctgcagaaattggcaacttgaggagtatcatggagattgatatgtccaacaatcatcttggcggtttgattcctcaagaactcggaatgctgcaaaatctgatgttgttaaatctcaaaaacaacaacataactggggatgtctcttcactgatgaactgcttcagcctcaatatcttaaatgtatcctataataatttggctggtgttgtacctactgataacaacttctcacggttttcgcctgacagctttttgggtaatccaggactttgtggatattggcttggttcttcgtgccgttcatctggccatcaacagaaaccactaatctcaaaggctgcaatacttggaattgccgtgggtgggcttgttatcctcctgatgatcttagtagcggtctgcaggcctcatagtccacctgttttcaaagatgtctctgttagcaaaccagtgagcaatgttccccccaagctggttatccttcatatgaacctttcccttcttgtatacgaggatataatgacgatgactgaaaacctgagtgagaagtacatcattgggtacggagcatccagcacggtttataaatgtgtttcgaagaaccgcaaaccagtggcagtaaaaaagctatatgcccactatccacagagcttcaaggaatttgaaactgagcttgagactgttggtagcatcaaacaccggaatctagtcagtcttcaaggatattccctatctcctgttggaaatcttctcttctacgattacatggaaaatggaagcctctgggatgttttgcatgaaggtccaactaagaagaaaaaacttgattgggaaactcgtctacgaattgctctaggtgcggcccaaggccttgcttatcttcatcatgactgtagcccacggataatacacagggatgtgaaatcaaaaaatatactccttgataaagattatgaggcacatcttacagactttggcattgctaagagtttgtgtgtttcaaaaactcacacgtccacctatgtcatgggaactattggctatatcgatcctgagtatgctcgcacctcccgtctcaatgaaaagtctgatgtctacagctatggcattgttctgcttgagctgctgaccggaaaaaagccagtggacaacgagtgcaatctccatcacttgatcttgtcaaagacggctaacaatgctgtcatggagacagtcgacccggacattgcagacacttgcaaggatcttggtgaggtcaagaaggtgttccagctggcgctcctttgcaccaagagacaaccatcggatcggccgacaatgcacgaggttgtgcgcgtcctggactgcctagttcgtcccgacccgccaccgaagtccgcacagcagctggccatgccgcagcggcctgctgtcccgagctacatcaacgagtatgtcagcttaagaggcaccagcgtgctctcctgcgccaactcgtcgtgtacttccgatgctgagctgtttctcaagtttggcgaggtcatttctcagaacacagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MTPAPAAASYRALVALLLVAVAVADDGSTLLEIKKSFRNVDNVL YDWAGGDYCSWRGVLCDNVTFAVAALNLSGLNLGGEISPAVGRLKGIVSIDLKSNGLS GQIPDEIGDCSSLKTLDLSFNSLDGDIPFSVSKLKHIESLILKNNQLIGVIPSTLSQL PNLKILDLAQNKLSGEIPRLIYWNEVLQYLGLRGNNLEGSISPDICQLTGLWYFDVKN NSLTGPIPETIGNCTSFQVLDLSYNKLSGSIPFNIGFLQVATLSLQGNMFTGPIPSVI GLMQALAVLDLSYNQLSGPIPSILGNLTYTEKLYMQGNKLTGPIPPELGNMSTLHYLE LNDNQLSGFIPPEFGKLTGLFDLNLANNNFEGPIPDNISSCVNLNSFNAYGNRLNGTI PPSLHKLESMTYLNLSSNFLSGSIPIELSRINNLDTLDLSCNMITGPIPSTIGSLEHL LRLNLSNNGLVGFIPAEIGNLRSIMEIDMSNNHLGGLIPQELGMLQNLMLLNLKNNNI TGDVSSLMNCFSLNILNVSYNNLAGVVPTDNNFSRFSPDSFLGNPGLCGYWLGSSCRS SGHQQKPLISKAAILGIAVGGLVILLMILVAVCRPHSPPVFKDVSVSKPVSNVPPKLV ILHMNLSLLVYEDIMTMTENLSEKYIIGYGASSTVYKCVSKNRKPVAVKKLYAHYPQS FKEFETELETVGSIKHRNLVSLQGYSLSPVGNLLFYDYMENGSLWDVLHEGPTKKKKL DWETRLRIALGAAQGLAYLHHDCSPRIIHRDVKSKNILLDKDYEAHLTDFGIAKSLCV SKTHTSTYVMGTIGYIDPEYARTSRLNEKSDVYSYGIVLLELLTGKKPVDNECNLHHL ILSKTANNAVMETVDPDIADTCKDLGEVKKVFQLALLCTKRQPSDRPTMHEVVRVLDC LVRPDPPPKSAQQLAMPQRPAVPSYINEYVSLRGTSVLSCANSSCTSDAELFLKFGEV ISQNTE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;6871..7239#6001..6374#5469..5816#5164..5292#5003..5076#4845..4916#4692..4760#4531..4602#4382..4453#4230..4301#4078..4149#3855..3926#3703..3774#3540..3611#3387..3458#3163..3234#2991..3062#2829..2897#2672..2743#2518..2589#2162..2233#2011..2082#1828..1899#1671..1742#1500..1571#1283..1412#371..449#gatgtgacatctcagtgtccatcggccatcgcctccttttctctctcctctcctctcaccatcttctcctacagcaaatcactcttctctctctctaaaaaacttcacaagcctactagcctactactaggcacgtagctagcccctgccactctatcgcccagcacaagccatacagctacccgctgtcactgcgccagcggccgcagctgcgcaaagaggagcgcgagaacccaagaaaccaaccaagagctgcgtcccataaaggcgcgctgcttcaatccatccatcttctgatcttccatatacttcgcttcctgtaaatgtcatcgccgccccggcggtgcctcgcagcggagcagcagtggccatgacgccggcgccggcggccgcctcctaccgcgctctcgtcgcgctcctgctcgtcgccgtcgccgttgccgatgatggtgggtggatggctggctagctagctggctgttggttttcggttttggttggtttcttgctggggggagtgtgtggttttgtgtactggggagcagagagattcaggggggagttggtttgatgggattagtattttattagtgttgcaagtactcttcctttttggaggcatggtgattctcttgtgggtgatagtcctactactattttttattgtcctaagatctccctaagaagagtctaatactttaactaatccttgtgagtgagttgtgacaagtgccacttccatctcttctcttccttcttgtactagaacacagttgctggcattttgtttatggtggtggtagccactaggtaatccaccaatatttcataatgtgattcttttttttttcactttctgttttgttctacaagggactccgttctgctttggtattctgttacttatcaagaagtcgtctggccatttttgaatctttgtgctcattgttcaaggaaggagctttatttcttcttttgtatgttactaccaatctgatgagccgaatgctgctgcccgcgtcaggaatctttaggctttgtagcaaccttttactttactattgtgctgatttttgtgctttccaatctgatgttcttgaactctttttttttttgtcttgaaggctaagaaacataaatctttatttattagtactactacgtaaatagattttttccggggcgaacaaatctgtgcagattgtgctgttctagaatccatttgctgttgcaggcttgcagtttgtgatctgtgttctaatgtggctgtatttgcattcagggtcgacgctgctggagatcaagaagtccttccgcaatgtggacaacgtactgtacgattgggccggcggcgactactgctcgtggcgcggcgtcctctgcgacaacgtcaccttcgccgtcgccgcgctgtaagaagaacaccaccgagttcgctacttttcttgctgacaagtccactggttcgtcactgatcatgcctgttgattgcaaagcagcaacctatccgggctcaacctcggaggcgagatctctccggccgtcggcaggttgaagggcatcgtctcgatgtatggaagacgtggtggtgtactttgttgttgtttggaaatgtggcttgctggtgcttggactcgcagtaacttgtgagcttgggtttgtacctgcagtgacttgaagtcgaatgggctgtctgggcagatccctgatgagattggcgattgttcatcactaaaaactctgtgagttgtgtggagtgtggacggcattatcgatttcttcttaacttacggagagaatgtgggttgatacatgtgtttggttcagggatttgtctttcaatagcttggatggggacattccgttctcagtatcgaagctgaagcacattgagagcttgtatggcctattcttgctggaattcatttattataagcagtgttgtatggacatgccgatcgtgttctgtgaaatagttttattcatgtgccggtgtttgtggttttctaggatattgaagaacaaccaactgatcggagtgatcccatcaacgctctcacagctcccaaatttgaagattttgtaagttgcgcgaaacttcttatttttgtggtgtttaattaccgtgctcccagttttctaattgttgtgtgccctgcagggacttggcacagaacaaactgagtggagagataccaagactgatatattggaacgaggttcttcaatacttgtaagtcatccttattttgcgctggaagtttgaagattgtgcagtagttctagtatatcagtattttattgtattttagttatgtacctacttgaatgcgatttctttttttttcttttttttgaaaatcatttgaatgtatttaagagcatgtcaaaagtttgttaagctggtaaaaatctatattgactttatgtgaattcactagcattttacgcaagttgcgttatgtaaaatactcatattttttttatgaattgtggctgaattaatattatcctcaggggattacgcggtaataatttagaaggcagcatctccccagatatatgccagttgactgggctttggtacttgtgagtatatgcatactatgttttcggcaaaatttgcctcgagtccaccgtattgtacttatgtttcaatcatttggaccagtgacgtaaagaacaacagcttgactgggccgataccagaaaccattgggaactgtacaagttttcaggtcttgtacgtatttgcagcttacccctcttaattacaacttttctgcttctgtgtttgtgaggttttcctaacatttctttcatgttagggatttgtcttacaataaactttctggatcaattcctttcaacattggtttcctacaagttgctacactgtatgtggtgattatcttgacaaaaaaaaactcaattaaatgtttaatacagtttttgctgatctgcatgattattcacattgtttgaattagatctttgcaagggaacatgtttactggtcctattccatcagttattggacttatgcaggctctcgctgtactgtaagtacaatgatgaactgaatattcatcatacaattggttattgataaatgaagctttcaagatattttaattcatcaatataactttactcttccagggatctgagttacaaccaattgtctggtcctattccatcgatactaggcaatttaacatacactgagaagctgtaagttttcttttctcctaaatcgttaggttgtgaatattttccctgatagattttctactgccagttcatagttaaaaaacaaaagccttagaaggttctgcttaggaatatttcttgtgtaaattaatatgttatatttgttccaataggtatatgcaaggcaataagttaacaggtccaataccacctgagcttggaaatatgtcaacccttcattacttgtaagtgcacgttttattttattcccatagcatattctgatttgttgcgacctgatttatgtcattgttgactaatttcagagaacttaacgataatcaacttagcgggttcattcctccagagttcggaaagctaacagggttatttgacttgtaagctcttttttcaaactcttcaaaatttgttatgttttctataatttattagtaaaacaagcggcttatcttactctttgcttcacagaaaccttgcaaacaacaactttgaaggtccaatccctgataacataagctcatgtgtgaatctcaatagcttgtaagcatgatttaattataattgttatggtggaacttacaatttcagatgcatgtatctaatgtggattttctttacagcaatgcttatggcaacagattaaatgggaccattcctccttcattgcataaacttgagagcatgacttatttgtaagttgtaacaattttaatttcttaaattgtgttgttacattatttttccttttgaacttctatacttgtttaatttttgtatttcacatagaaaaaccttgtttcccatgtttgattagtaccatatgttcatctgttcttttcttaggaatttgtcatcaaattttctaagtggttctattcctattgagctatcgagaatcaacaatttggacaccttgtaagtcctgttagctccatctatgaattcgaaaatatctgttctatcctattgtgtgtgtgatgaggtacctgtatcagggatttatcctgtaacatgattactggcccaattccatcaaccattgggagtttggagcatctattaagactgtaggttttgttgtccccatcttgtatgtatctattatagtgtcttacagtgtttgatgttattgaacttgtttgttcagtaacttgagcaacaatggtctagtaggattcattcctgcagaaattggcaacttgaggagtatcatggagatgtaagtatttgcttaggtgtgaattttttaaccatctagtcccataggtactataccccatatttgtggtttttcagtgatatgtccaacaatcatcttggcggtttgattcctcaagaactcggaatgctgcaaaatctgatgttgttgtaagtagtacctttcatgtcagccttctttttaaacccataaggacattgaatctgtagcctgagagacatctgtctatcattctcagaaatctcaaaaacaacaacataactggggatgtctcttcactgatgaactgcttcagcctcaatatcttgtaagttgaataaccagaaaatttttgaagcatatgctccagtatatgttttttgctcacattatctatctgttttatgatcagaaatgtatcctataataatttggctggtgttgtacctactgataacaacttctcacggttttcgcctgacaggtagtgtgctccatcatttccatcactatcatgttcatttactcagccttggaaatagcagtttaacaatgtgatacttttcttagctttttgggtaatccaggactttgtggatattggcttggttcttcgtgccgttcatctggccatcaacagaaacgtaacaaatattccattcatatcttacataattttttcatacctaatagacaaagtcaacttttcgttacctacattttatttgcagcactaatctcaaaggctgcaatacttggaattgccgtgggtgggcttgttatcctcctgatgatcttagtagcggtctgcaggcctcatagtccacctgttttcaaagatgtctctgttagcaaaccaggtaatgcatctgcaaggagttttgctcaacaaatcaactcatttctctcctgccaagttcaatattaggatactatcttgtccagcaattattgtatttagtcaattgtttgtttttacctgataactgggttaatggtagagcaacactaaagcgctgtatatttgctgtcacagtgagcaatgttccccccaagctggttatccttcatatgaacctttcccttcttgtatacgaggatataatgacgatgactgaaaacctgagtgagaagtacatcattgggtacggagcatccagcacggtttataaatgtgtttcgaagaaccgcaaaccagtggcagtaaaaaagctatatgcccactatccacagagcttcaaggaatttgaaactgagcttgagactgttggtagcatcaaacaccggaatctagtcagtcttcaaggatattccctatctcctgttggaaatcttctcttctacgattacatggaaaatggaagcctctgggatgttttgcatggtaagtgccgactgccaactcattgcacagtttgattgtcattgcaatttgcacagaagcaccaacatgccattctcaaaagcatgatatttgacacattgatatttgcttctgttgattgcaataccaaattgttacatgacatcttgttaccatgttaaaatcttttttttcattattctagaaggtccaactaagaagaaaaaacttgattgggaaactcgtctacgaattgctctaggtgcggcccaaggccttgcttatcttcatcatgactgtagcccacggataatacacagggatgtgaaatcaaaaaatatactccttgataaagattatgaggcacatcttacagactttggcattgctaagagtttgtgtgtttcaaaaactcacacgtccacctatgtcatgggaactattggctatatcgatcctgagtatgctcgcacctcccgtctcaatgaaaagtctgatgtctacagctatggcattgttctgcttgagctgctgaccggaaaaaagccagtggacaacgagtgcaatctccatcacttggtaactatgattcatgctcttgtgattcttctgttaatctgtcattttcttcagcttattttactttggattctggtagccctcaataattgctgaatgatgcccattaggcttgtgttcctacatggggaccaacaacatgaaacctatcctgtgagtagatctatttgcactgtttacgtgtggtggattgatccatcgcatatggctgttgaaagctccattatcaattgcagtgtttaagtcccctaaagcctgccacttttgtcccctgtcatcaattatgtggattgaaagctacaatttagtgtcataaaaattcctcttgcagccatttacattttttacgccttcacttggcatttaaccgacctccgacttggagagtcaccctaatttctcattgattttacatgcttatatggcttttcatttatcggttttccttgctaatgtacagactgttacaattcttggtctactgttgcaaatgcagatcttgtcaaagacggctaacaatgctgtcatggagacagtcgacccggacattgcagacacttgcaaggatcttggtgaggtcaagaaggtgttccagctggcgctcctttgcaccaagagacaaccatcggatcggccgacaatgcacgaggttgtgcgcgtcctggactgcctagttcgtcccgacccgccaccgaagtccgcacagcagctggccatgccgcagcggcctgctgtcccgagctacatcaacgagtatgtcagcttaagaggcaccagcgtgctctcctgcgccaactcgtcgtgtacttccgatgctgagctgtttctcaagtttggcgaggtcatttctcagaacacagagtagaatagtctgcagaaatcgtcatccgatcttgggattcaggcaagcagctggtgaagctaatcaaggtagtgaagtgaactcacgttttgtgactttgtccacatgttgggtgcccagaaaattaacaagagaattctcaagggttgtggctggtgttctttactgtaaaaaaaatgcgtcctacttctttcttcttcctttttttttatctcgtttttgtctaacttaggaggtgtccatgaatctagtactaaatctcaagacagcttcttctgttctgtaacatttctgggattggaatgctggaactggtattactaatagtctctcggtttggacaaattcgcagggtggtgtttcagtttc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063622.1 RefSeq:Os06g0203800]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 6]]<br /> [[Category:Chromosome 6]]</div>

LIZHAO