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Os01g0782100

2014-05-28T03:00:20Z

Liangdan2013: /* References */

Please input one-sentence summary here.
This rice blast resistance genes was found by two Japanese scholars Imbe and Matsumoto in 1985, they called this gene Pi-sh,this gene has the moderate resistance to the blast fungus strain Kyu77-07A in rice variety Shin-2.this gene has been characterized by using the method of classical genetic.And this gene is linking to pi-t.
==Annotated Information==
===Function===
The genes are accounting for the moderate resistance of the rice variety Shin 2 and some other varieties to the blast fungus strain Kyu 77-07A. Aichi Asahi (Pi-a) and carried out in the present study. Yashiro-mochi (Pi-ta), were found to be susceptible to the strain, while Ishikari Shirol*.e (Pi-i), Kanto 51 (Pi-k), Tsuyuake (Pi-k'!b), Fukunishiki (Pi-z) and Toride I (Pi-zt) were resistant, and Shin 2 (Pi-ks) and Pi N0.4 (Pi-ta2) moderately resistant. Therefore, Kyu 77-07A was classified as one of the strains belonging to race 102. Howver, the variety Reiho with the resistance gene Pi-ta2 was susceptible to this strain. Based on the knovn facts mentioned above, the objectives 0L the present study were' to determinA- whether Pi-ks is the gene responsible for the moderate resistance of Shin 2' to Kyu 77-07A along with the reason for the different reactions to Kyu 77-07A betweerL Pi N0.4 and Reiho which both have the same resistance gene Pi-ta2. For the gene analysis, the F2 or F3 plants of the crosses were inoculated with Kyu 77-07A and three other fungus strains using the spraying rnethod when the plants were at the four- to five-1eaf stage. Two varieties, Mineyutaka. and Saikai 155 belonging to the Shin 2 type varieties, were used as the representative parents of the varieties susceptible to Kyu 77-07A for the crosses( Y. Koide,2010).

===Expression===
To identify novel factors required for R gene-mediated resistance in rice, we used rice insertional mutant lines, induced by the endogenous retrotransposon Tos17, in a forward screen involving the rice blast fungus Magnaporthe oryzae. We inoculated 41,119 mutant lines with the fungus using a high throughput procedure, and identified 86 mutant lines with diminished resistance. A genome analysis revealed that 72 of the 86 lines contained mutations in a gene encoding a nucleotide binding site (NB) and leucine rich repeat (LRR) domain-containing (NLR) protein. A genetic complementation analysis and a pathogenesis assay demonstrated that this NLR gene encodes Pish, which confers resistance against races of M. oryzae containing avrPish. The other 14 lines have intact copies of the Pish gene, suggesting that they may contain mutations in the signaling components downstream of Pish. The genome analysis indicated that Pish and its neighboring three NLR genes are high similar to one another and are tandemly located. An in silico analysis of a Tos17 flanking sequence database revealed that this region is a "hot spot" for insertion. Intriguingly, the insertion sites are not distributed evenly among these four NLR genes, despite their similarity at the sequence and expression levels.
In this work we isolated the R gene Pish, and identified several other mutants involved in the signal transduction required for Pish-mediated resistance. These results indicate that our genetic approach is efficient and useful for unveiling novel aspects of defense signaling in rice. Furthermore, our data provide experimental evidence that R gene clusters have the potential to be highly preferred targets for transposable element insertions in plant genomes. Based on this finding, a possible mechanism underlying the high variability of R genes is discussed.
Primer pairs utilized in this work(Akira Takahashi,2010).

[[File:sequence.jpg]]

===Evolution===
A study was conducted to investigate the relationship between phenotype of blast resistance and resistance gene analog polymorphism of rice, and search molecule hereditary basis of broad spectrum and durable resistance.
【Method】 Comparison of clustering analysis was investigated using spectrum of resistance to blast and polymorphism of resistance gene analog (RGA) in 25 varieties for blast resistance identification and 20 varieties lines).
【Result】 The resistance spectrum clustering analysis showed that the 45 varieties (lines) could be divided into group A and group B with the genetic similarity coefficient of 0.450. Group A and group B could be divided into subclassⅰ, subclass , ⅱ subclass , ⅲ subclass ⅳ, respectively, with 0.618 genetic similarity coefficient. The RGA-PCR clustering analysis showed that proposed the 45 varieties (lines) could be divided into group Ⅰ and group Ⅱ which clearly inclined the Indica-japonica differentiation with 0.620 genetic similarity coefficient. Group Ⅰ could be divided into six subclasses and group Ⅱ could be divided into seven subclasses with 0.783 genetic similarity coefficient. The resistance spectrum clustering analysis showed that some varieties with similar resistance spectrum could finely fall into the same group, while the RGA-PCR clustering analysis showed that some varieties with the similar genetic background could fall into the same group. For some varieties with low resistance frequency or high resistance frequency, there was a better corresponding relationship between the resistance spectrum clustering and the RGA-PCR clustering. General comparison of clustering analysis showed that here was no parallelism relationship between group and group in two different types of the clustering.
【Conclusion】 It could more accurately reflect their enetic background to test resistance to single strain and analysis on RGA polymorphism for resistance parents, and avoid applying the same source of resistance gain and again, and enrich rice resistance germplasm, and breed durable resistance varieties(Tokio IMBE,1985).

You can also add sub-section(s) at will.

==Labs working on this gene==
1.Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Ibaraki, 305-8602，Japan.
2.International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines;
3.Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, Japan;
4.Indonesian Center for Rice Research (ICRR), JL. Raya Muara No. 25A Ciapus Bogor, Subang, West Java, Indonesia;
5.Agricultural Genetics Institute,Conhue, Tuliem, Hanoi, Vietnam;
6.Faculty of Agricultural Sciences, University of Burundi, BP 2940 Bujumbura, Burundi;

==References==
1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta
Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.)
Plant Breeding, 2010, 129(6): 670-675
2. Akira Takahashi;Nagao Hayashi;Akio Miyao;Hirohiko Hirochika
Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging
BMC Plant Biology, 2010, 10: 175
3. Tokio IMBE;Shohei MATSUMOTO
Inheritance of Resrstance of Rice Vanetles to the Blast Fungus Strains Virulent to the Variety "Reiho"
Japanese Journal of Breeding, 1985, 35(0): 332-339.
4.鄂志国, 张丽靖, 焦桂爱, 等. 稻瘟病抗性基因的鉴定及利用进展[J]. 中国水稻科学, 2008, 22(5): 533-540.
Further reading:
5.杨勤忠, 林菲, 冯淑杰, 等. 水稻稻瘟病抗性基因的分子定位及克隆研究进展[J]. 中国农业科学, 2009, 42(5): 1601-1615.
6.任鄄胜, 肖培村, 陈勇, 等. 水稻品种稻瘟病抗性和抗病基因同源序列多态性分析[J]. 中国农业科学, 2009, 42(1): 1-9.

==Structured Information==
{{JaponicaGene|
GeneName = Os01g0782100|
Description = Similar to NBS-LRR protein (Fragment)|
Version = NM_001185663.1 GI:297720460 GeneID:9271927|
Length = 8696 bp|
Definition = Oryza sativa Japonica Group Os01g0782100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:34892692..34901387|
CDS = 34896973..34900845|
GCID = <gbrowseImage1>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaatgtgtttacaatgacaagagggtacaagaacattttgacgtcaggatatgggtctgcatctcacgcaaacttgatgttcatcgccatacacgtgagattatcgagtcagcaacaaatggagagtgccctcgtgtggacaatcttgatactctccagtgcagattgaaggacataatgcaaaaatcagagaaattcctgcttgtgttggatgacgtctggtttgatgaatccgtcaatgagagggaatgggaccagttgcttgatcctcttgtttctcagcaggaggggagcagagttttggtgacttctagacgagatgtacttccagctgctcttcactgtaaggatgttgttcatttggaaaacatggaagatgccgagttcttggctctattcaaataccatgctttctctggaacagaaatcagaaatccacagttgcatgcacggctagaagaggtcgcagagaagattgctaaaaggttaggacaatcgcctttagcagcaaggacagtaggctcccagctgagcaggaataaggatattgccatatggaaaagtgctctaaatattgaaaacttaagtgagcccatgaaagctctgttgtggagttataataaattagattcacgtctgcagagatgttttctgtactgcagcttatttccaaaaggtcacaagtataaaattgatgagatggttgacctttgggtggcagaggggctagttgattcacgcaaccagggggataagagaattgaagatattggcagggattacttcaatgagatggtgtcaggatctttctttcaaccagtttctgaaagatatatgggtacatggtacattatgcatgatctgcttcatgatttggcagagtcactgactaaagaagactgcttcagattagaagatgatggggtgaaagagataccagccactgttcgacatctatctatttgtgttgacagtatgaaattccataagcaaaagatctgcaagctacgttatttacgcactgttatctgcattgaccctctcatggatgacggagatgatatttttaatcagctattgaagaatctgaagaagctacgtgtactacatttgtcgttttacaacagtagcagcttgcctgaatgtattggtgagctgaagcaccttcgatatttgagtatcatcagcacattgatttctgaactgccaagatcattgtgtactcttttccacttggagctacttcatttgaatgacaaggtcaagaatttgccggacagactctgtaatttaagaaagttacgacgtcttgaagcatatgatgacagaaacagaatgtataaattgtatagagcagctctccctcaaattccttacataggcaagctatctttgctgcaagatattgatggattttgcgtgcaaaagcagaagggatatgagttgcgacaactgagggacatgaacaagctcggtggtaatttgcgtgtcgttaatcttgaaaatgtaactggaaaggatgaagcctcagagtcgaagttgcatcagaaaactcatcttagaggtttgcatctctcctggaatgatgtggatgacatggatgtttcacatttggagattctagaaggtttgaggccgccatctcaattggaggaccttacaatcgaaggttacaaatccaccatgtacccaagctggttacttgatggatcctattttgagaatctagagtcttttacgcttgctaattgctgtgtgataggaagcctaccacccaataccgagatctttaggcactgtatgacacttacccttgagaatgtcccgaatatgaagacactaccttttcttccagaaggtctcacaagcctatcaattgaggggtgcccactgcttgtgttcactactaataacgatgaactggaacaccatgattatagggagagcatcacgagggcaaacaacctggaaacacaacttgttttgatatgggaagcgaattccgattcagatattaggagcacattgtcgtccgaacactcatccatgaagaagttgacggaattgatggatactgatatgtcgggaaatcttcaaaccattgaaagcgctttagaaatagagagagatgaagccttggttaaagaagatatcatcaaagtatggctctgttgccacgaggagaggatgagattcatttattcaaggaaggctgggttgccgttggttctaccatctggactatgcgtactctctctttcctcctgtagtattacagatggagctttagctatttgccttggtgggcttacttcacttagaaatttgttcttaacagagattatgactttaactacgcttccaccggaagaggtcttccaacatttgggcaatcttagatacttggtcattcgttcctgctggtgtctcagatcattcgggggcttgcgatctgctacctctctttcagaaataagattattttcctgtccttctttacagttggcacgtggcgcagaatttatgcaaatgtcccttgagaagctatgtgtatacaactgtgtgctttcagctgacttcttctgcggtgactggccacatctgaataatattgggttatgtgggtgtagaagctctgcgtccttgtatgttggtgatcttacctccctcaaatcattctcactatatcatttgccagatttatgcgtgctggaaggtttgtcttacctacagcttcaccacgtgcatttgatagatgttccaaagcttactaccgagagcatctcacagttccgcgtacagcgttcgctctatattagcagttccgttatgctcaaccacatgatctccgctgaaggtttcaaagttccagggtttctctctcttgaaagctgcaagaagccatccgtttcattcgaagaatctgcaaatttcacatccgtcaagtgcctgaggttatgtaattgtgaaatgaggtcactaccaggaaatatgaagtgcctatccagtctaacgaaactcgatatctatgactgccccaacataacatctctaccagatctgccgtcctccctccagcatatatgtatatggggttgtgagcttttgaagaagagctgcagggcacctgatggagaaagctggccaaagattgcgcatatccgctggaaggaattcagatga</cdnaseq>|
AA = <aaseq>MAEVVLAGLRLAATPICVKLLCNASTCLGVDMTRELHELETIII PQFELVIEAAEKGNHRAKLDRWLRELKQAFYNAEDLLDEHEYNILKCKAKHKDSLVKD STQVHDSSISNILKQPMRAVSSRMSNLRPENRKILCQLNELKTMLEKAKEFRELIHLP AGNSLEGPSVPTIVVPVVTSLLPPRVFGRNMDRDRIIHLLTKPMATVSSSVGYSGLAI VAHGGAGKSTLAQCVYNDKRVQEHFDVRIWVCISRKLDVHRHTREIIESATNGECPRV DNLDTLQCRLKDIMQKSEKFLLVLDDVWFDESVNEREWDQLLDPLVSQQEGSRVLVTS RRDVLPAALHCKDVVHLENMEDAEFLALFKYHAFSGTEIRNPQLHARLEEVAEKIAKR LGQSPLAARTVGSQLSRNKDIAIWKSALNIENLSEPMKALLWSYNKLDSRLQRCFLYC SLFPKGHKYKIDEMVDLWVAEGLVDSRNQGDKRIEDIGRDYFNEMVSGSFFQPVSERY MGTWYIMHDLLHDLAESLTKEDCFRLEDDGVKEIPATVRHLSICVDSMKFHKQKICKL RYLRTVICIDPLMDDGDDIFNQLLKNLKKLRVLHLSFYNSSSLPECIGELKHLRYLSI ISTLISELPRSLCTLFHLELLHLNDKVKNLPDRLCNLRKLRRLEAYDDRNRMYKLYRA ALPQIPYIGKLSLLQDIDGFCVQKQKGYELRQLRDMNKLGGNLRVVNLENVTGKDEAS ESKLHQKTHLRGLHLSWNDVDDMDVSHLEILEGLRPPSQLEDLTIEGYKSTMYPSWLL DGSYFENLESFTLANCCVIGSLPPNTEIFRHCMTLTLENVPNMKTLPFLPEGLTSLSI EGCPLLVFTTNNDELEHHDYRESITRANNLETQLVLIWEANSDSDIRSTLSSEHSSMK KLTELMDTDMSGNLQTIESALEIERDEALVKEDIIKVWLCCHEERMRFIYSRKAGLPL VLPSGLCVLSLSSCSITDGALAICLGGLTSLRNLFLTEIMTLTTLPPEEVFQHLGNLR YLVIRSCWCLRSFGGLRSATSLSEIRLFSCPSLQLARGAEFMQMSLEKLCVYNCVLSA DFFCGDWPHLNNIGLCGCRSSASLYVGDLTSLKSFSLYHLPDLCVLEGLSYLQLHHVH LIDVPKLTTESISQFRVQRSLYISSSVMLNHMISAEGFKVPGFLSLESCKKPSVSFEE SANFTSVKCLRLCNCEMRSLPGNMKCLSSLTKLDIYDCPNITSLPDLPSSLQHICIWG CELLKKSCRAPDGESWPKIAHIRWKEFR</aaseq>|
DNA = <dnaseqindica>4282..8154#gggatctcccgccgttcggagacctaggggagcaagggggagactagagaaggttgcgctccatgaagtcatggtgcaccttaggtatcttgaaaggttttaccaagcgacctggttattgaagcgtggagaagaggctccagtgcggcaggagaaagcagatgagtagaggtacgatctcttcaggagaaagcagagcccagctgcccaggtggtggggagagattatcaaatttttcggtggtggtttggcacgggactctgccgcttcgctgccttcaaggctcaagctcagggtgactggcggcatctgacaggttcatttcctcttgcttatgtcttttagttgcaaatcaattaccattggttgtgggtttgaattattgggcgaaatgctcatgcgttggtcttgaaatttagctttttatctatctctatgttttacattataagtcgctttaatttttttctaattatttttttaagtttgatcaagttcatataaaaatgcaacaacattttcaactcaaaacaaatatattaatataatatattcaatgctatatgtaatgaaactaatttagtgttatgaatgttactatatttttttataaagttggttaaatctaaaaaggtttaattaaaaaaaatcaaaactacttataatacaagtaaaatagagagaattgcatagaagtggaagtgtgtcggtgtctgtactggttctactcacctagtcacctccctgccactgctattctgctaatagtgttagagaatacgcacgcacagataatggcaaggacgtcatttagaagagcccactagtgcgtgtcatgttgcttatgtgcaaattttatacactttcttgactgaccaaaggttgtatgcggtcattttttttttctctcggtgctagctttaatatacggaaagccgtttagagaagatggcatgacaataatggagcatatatatataaactagctcgtaccctgcggcgttgctgcgggtagatattgtaattgcaagtgtccagagaataacaaataaaattttccatggaacaatcatgatggccaaattgtataatatatggctgaaagttcacattatctaaaacaaatatttttcgctgcggtcggagtgtatattaagaaggagagagtttaagcaaactgtacaagagaacaatcagagccaaagctcacatacagaaccaggacactggttcgccacacacacatggttcaaacttcaaacattactgggagtctgatatacatttctttcttgtcttcctgcaaatttgtgttggcactagcttcatacagacctcaactctactgatccaagaacaacatgtagggctacatcctacatgtatacccaaatacccaatacaaacacaaaatagacgaagctaccctgcaatgcaatcagtaataagatcaatagttgataccgaagccaccagccagcatttcgggtaaacatagagcaacaatgaatataagcaagtctcttaagaattacgttctcattttgataaactggcaagcacgggtgaattttgcatcaacacccaacggaaatttgattagcattccttgaaatatgtcactgttgatgaaggtaaaaaagtaaacacaaagtgaagcccatagagcttcagtttagttcaggcatcaacatagtttaactcatgaacctccaaattaaataaattatttatttcattattatatatcataccttggtacatttgtcagcctcacggcagtagattttgcccatgttgcagtaagttacacacacaaacaccaaatatgctagtttttaaactattcttgtttcactagatttaattgcatttggttttttactatgctatttcggagttgtttaatttgatagtgggggccatagagtataatgaaaaacagcaaggtaatgagaaccagagcatagtccggctagaatattttaggagtccttttaatgaggtccagcttgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattctccaatttctccaaattgtaacatggtatcagacccttgttccaaaaaccctagccaccaaatccgatcatcaccggcgcaccggcggcggtgagccgccggcgagccatcatcatcgtcctccttcatccctcaacgtttttgtctccaagcagcaagcctgcagttcgtcgtcgtcaccgtgttcctgttcgccggtcgcagccgccgccagttgcggcgccgcgcccgttcccacttctccacccacgcagccacgccgtcgtggtgttcatccacgctgctcgtcgcggggctcgttcttgccgccgttcgagagccgccgctgtggcctcgtcaatctacatcaaggcatcaagcaacatcagtttttggcccatttcttgtcagttagcatcagcaaaccatcgtcctctctgatctgcagcagcaacaacaacaactcccgttcacctcctagtagatgcagcagctagcccattgttgtctcctaatttcagcagtagaatttccatgttccattgtttgtgttgcaacatgcattctgcagattgtcatcaagcttgtattccaccacatgtgttagtcagtttttgcgcttgctaatcgctgccatccactactcactcatcaatcatcaccatgctttgttattgttagctacttattataatccaattttacttctccaaattatcatgatggcttgtagtagtcactggttaggtcattagctgcttgcttgctactgtcaacatgttcactagcatcctttgtgtcttttgctagtaatgtgcatctctgcttgcagtccatctatcaatattgcctttttgctatatccatcaatccgtgttctttactaatcaaattcctgtgtttcctaccgagtccagcaaaatccattgcatatccttcattttgtcgaagctgtgccagtccatctcttcatggtcatttcatcatcaccagctccccgtcggagctggccttcattgtcacttcatatcttcatcatctccatgtcggagcttgtcatcatcatcatcaatcagtgaaggtccgaagaacagtttgtgaagggccttcatcaatatcaatgtctcatcatcaatcaaaggaccgaagaacagcttgtgaaggtccattgtcatcatcatcaacatctccgaatcaatgaagagccgaagaacgattcgtgaaggcttcaaggtaccgagggccgaagaacagtttgtgaaggctccgaggtccatttcattgaggagggggtcttttcttcttcatcatccaagaattgacacacttcgacgacatcatcaagactttggagataggctaatatttagattgtgttctcatgtttagtttacctttcaaatgcaatgttattacatacacattgcatttgagtgggggtgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattccccaatttctccaaattgtgacacagctcatctgaatattagagaaatataaatttgccactaacatttaacaatgtcccacttgtctactccctccgtcaaaaaaaaccaaacctaagacttagatgtgatataatctagtatctgtctagattcgttgtactagattatggcttaaatactagattaagttttttttgggacggagggagtatgtctaaccaaaataacacaattgatttgcatattgtgttttgcctcgcattgtgccgaagcaatcttatctgtggcaattggtagagttcttcctctcacaccttctctgcaatacaaacgcatctggctctggtaaccttgcactcctctcttagtatttctttccttcacgctttcttgtctctgcttaagttctctatcgtttggtaccatcatgcattaattcctttttcctctatttcccactccaaacatttccataaccactcacatacacaaaaataaaaaaccagacagagagaaaaaaacttgttttgggagttctgttgttatcttcctcacagaagaaaaatctctccatcaccatctgttcttatgccctgcttctattcattcacttgttcacagctgcagtagtgctgttccatggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaat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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185663.1 RefSeq:Os01g0782100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

Os01g0782100

2014-05-28T02:59:44Z

Liangdan2013: /* Evolution */

Please input one-sentence summary here.
This rice blast resistance genes was found by two Japanese scholars Imbe and Matsumoto in 1985, they called this gene Pi-sh,this gene has the moderate resistance to the blast fungus strain Kyu77-07A in rice variety Shin-2.this gene has been characterized by using the method of classical genetic.And this gene is linking to pi-t.
==Annotated Information==
===Function===
The genes are accounting for the moderate resistance of the rice variety Shin 2 and some other varieties to the blast fungus strain Kyu 77-07A. Aichi Asahi (Pi-a) and carried out in the present study. Yashiro-mochi (Pi-ta), were found to be susceptible to the strain, while Ishikari Shirol*.e (Pi-i), Kanto 51 (Pi-k), Tsuyuake (Pi-k'!b), Fukunishiki (Pi-z) and Toride I (Pi-zt) were resistant, and Shin 2 (Pi-ks) and Pi N0.4 (Pi-ta2) moderately resistant. Therefore, Kyu 77-07A was classified as one of the strains belonging to race 102. Howver, the variety Reiho with the resistance gene Pi-ta2 was susceptible to this strain. Based on the knovn facts mentioned above, the objectives 0L the present study were' to determinA- whether Pi-ks is the gene responsible for the moderate resistance of Shin 2' to Kyu 77-07A along with the reason for the different reactions to Kyu 77-07A betweerL Pi N0.4 and Reiho which both have the same resistance gene Pi-ta2. For the gene analysis, the F2 or F3 plants of the crosses were inoculated with Kyu 77-07A and three other fungus strains using the spraying rnethod when the plants were at the four- to five-1eaf stage. Two varieties, Mineyutaka. and Saikai 155 belonging to the Shin 2 type varieties, were used as the representative parents of the varieties susceptible to Kyu 77-07A for the crosses( Y. Koide,2010).

===Expression===
To identify novel factors required for R gene-mediated resistance in rice, we used rice insertional mutant lines, induced by the endogenous retrotransposon Tos17, in a forward screen involving the rice blast fungus Magnaporthe oryzae. We inoculated 41,119 mutant lines with the fungus using a high throughput procedure, and identified 86 mutant lines with diminished resistance. A genome analysis revealed that 72 of the 86 lines contained mutations in a gene encoding a nucleotide binding site (NB) and leucine rich repeat (LRR) domain-containing (NLR) protein. A genetic complementation analysis and a pathogenesis assay demonstrated that this NLR gene encodes Pish, which confers resistance against races of M. oryzae containing avrPish. The other 14 lines have intact copies of the Pish gene, suggesting that they may contain mutations in the signaling components downstream of Pish. The genome analysis indicated that Pish and its neighboring three NLR genes are high similar to one another and are tandemly located. An in silico analysis of a Tos17 flanking sequence database revealed that this region is a "hot spot" for insertion. Intriguingly, the insertion sites are not distributed evenly among these four NLR genes, despite their similarity at the sequence and expression levels.
In this work we isolated the R gene Pish, and identified several other mutants involved in the signal transduction required for Pish-mediated resistance. These results indicate that our genetic approach is efficient and useful for unveiling novel aspects of defense signaling in rice. Furthermore, our data provide experimental evidence that R gene clusters have the potential to be highly preferred targets for transposable element insertions in plant genomes. Based on this finding, a possible mechanism underlying the high variability of R genes is discussed.
Primer pairs utilized in this work(Akira Takahashi,2010).

[[File:sequence.jpg]]

===Evolution===
A study was conducted to investigate the relationship between phenotype of blast resistance and resistance gene analog polymorphism of rice, and search molecule hereditary basis of broad spectrum and durable resistance.
【Method】 Comparison of clustering analysis was investigated using spectrum of resistance to blast and polymorphism of resistance gene analog (RGA) in 25 varieties for blast resistance identification and 20 varieties lines).
【Result】 The resistance spectrum clustering analysis showed that the 45 varieties (lines) could be divided into group A and group B with the genetic similarity coefficient of 0.450. Group A and group B could be divided into subclassⅰ, subclass , ⅱ subclass , ⅲ subclass ⅳ, respectively, with 0.618 genetic similarity coefficient. The RGA-PCR clustering analysis showed that proposed the 45 varieties (lines) could be divided into group Ⅰ and group Ⅱ which clearly inclined the Indica-japonica differentiation with 0.620 genetic similarity coefficient. Group Ⅰ could be divided into six subclasses and group Ⅱ could be divided into seven subclasses with 0.783 genetic similarity coefficient. The resistance spectrum clustering analysis showed that some varieties with similar resistance spectrum could finely fall into the same group, while the RGA-PCR clustering analysis showed that some varieties with the similar genetic background could fall into the same group. For some varieties with low resistance frequency or high resistance frequency, there was a better corresponding relationship between the resistance spectrum clustering and the RGA-PCR clustering. General comparison of clustering analysis showed that here was no parallelism relationship between group and group in two different types of the clustering.
【Conclusion】 It could more accurately reflect their enetic background to test resistance to single strain and analysis on RGA polymorphism for resistance parents, and avoid applying the same source of resistance gain and again, and enrich rice resistance germplasm, and breed durable resistance varieties(Tokio IMBE,1985).

You can also add sub-section(s) at will.

==Labs working on this gene==
1.Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Ibaraki, 305-8602，Japan.
2.International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines;
3.Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, Japan;
4.Indonesian Center for Rice Research (ICRR), JL. Raya Muara No. 25A Ciapus Bogor, Subang, West Java, Indonesia;
5.Agricultural Genetics Institute,Conhue, Tuliem, Hanoi, Vietnam;
6.Faculty of Agricultural Sciences, University of Burundi, BP 2940 Bujumbura, Burundi;

==References==
1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta
Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.)
Plant Breeding, 2010, 129(6): 670-675
2. Akira Takahashi;Nagao Hayashi;Akio Miyao;Hirohiko Hirochika
Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging
BMC Plant Biology, 2010, 10: 175
3. Tokio IMBE;Shohei MATSUMOTO
Inheritance of Resrstance of Rice Vanetles to the Blast Fungus Strains Virulent to the Variety "Reiho"
Japanese Journal of Breeding, 1985, 35(0): 332-339.
4.鄂志国, 张丽靖, 焦桂爱, 等. 稻瘟病抗性基因的鉴定及利用进展[J]. 中国水稻科学, 2008, 22(5): 533-540.
5.杨勤忠, 林菲, 冯淑杰, 等. 水稻稻瘟病抗性基因的分子定位及克隆研究进展[J]. 中国农业科学, 2009, 42(5): 1601-1615.
6.任鄄胜, 肖培村, 陈勇, 等. 水稻品种稻瘟病抗性和抗病基因同源序列多态性分析[J]. 中国农业科学, 2009, 42(1): 1-9.
==Structured Information==
{{JaponicaGene|
GeneName = Os01g0782100|
Description = Similar to NBS-LRR protein (Fragment)|
Version = NM_001185663.1 GI:297720460 GeneID:9271927|
Length = 8696 bp|
Definition = Oryza sativa Japonica Group Os01g0782100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:34892692..34901387|
CDS = 34896973..34900845|
GCID = <gbrowseImage1>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaatgtgtttacaatgacaagagggtacaagaacattttgacgtcaggatatgggtctgcatctcacgcaaacttgatgttcatcgccatacacgtgagattatcgagtcagcaacaaatggagagtgccctcgtgtggacaatcttgatactctccagtgcagattgaaggacataatgcaaaaatcagagaaattcctgcttgtgttggatgacgtctggtttgatgaatccgtcaatgagagggaatgggaccagttgcttgatcctcttgtttctcagcaggaggggagcagagttttggtgacttctagacgagatgtacttccagctgctcttcactgtaaggatgttgttcatttggaaaacatggaagatgccgagttcttggctctattcaaataccatgctttctctggaacagaaatcagaaatccacagttgcatgcacggctagaagaggtcgcagagaagattgctaaaaggttaggacaatcgcctttagcagcaaggacagtaggctcccagctgagcaggaataaggatattgccatatggaaaagtgctctaaatattgaaaacttaagtgagcccatgaaagctctgttgtggagttataataaattagattcacgtctgcagagatgttttctgtactgcagcttatttccaaaaggtcacaagtataaaattgatgagatggttgacctttgggtggcagaggggctagttgattcacgcaaccagggggataagagaattgaagatattggcagggattacttcaatgagatggtgtcaggatctttctttcaaccagtttctgaaagatatatgggtacatggtacattatgcatgatctgcttcatgatttggcagagtcactgactaaagaagactgcttcagattagaagatgatggggtgaaagagataccagccactgttcgacatctatctatttgtgttgacagtatgaaattccataagcaaaagatctgcaagctacgttatttacgcactgttatctgcattgaccctctcatggatgacggagatgatatttttaatcagctattgaagaatctgaagaagctacgtgtactacatttgtcgttttacaacagtagcagcttgcctgaatgtattggtgagctgaagcaccttcgatatttgagtatcatcagcacattgatttctgaactgccaagatcattgtgtactcttttccacttggagctacttcatttgaatgacaaggtcaagaatttgccggacagactctgtaatttaagaaagttacgacgtcttgaagcatatgatgacagaaacagaatgtataaattgtatagagcagctctccctcaaattccttacataggcaagctatctttgctgcaagatattgatggattttgcgtgcaaaagcagaagggatatgagttgcgacaactgagggacatgaacaagctcggtggtaatttgcgtgtcgttaatcttgaaaatgtaactggaaaggatgaagcctcagagtcgaagttgcatcagaaaactcatcttagaggtttgcatctctcctggaatgatgtggatgacatggatgtttcacatttggagattctagaaggtttgaggccgccatctcaattggaggaccttacaatcgaaggttacaaatccaccatgtacccaagctggttacttgatggatcctattttgagaatctagagtcttttacgcttgctaattgctgtgtgataggaagcctaccacccaataccgagatctttaggcactgtatgacacttacccttgagaatgtcccgaatatgaagacactaccttttcttccagaaggtctcacaagcctatcaattgaggggtgcccactgcttgtgttcactactaataacgatgaactggaacaccatgattatagggagagcatcacgagggcaaacaacctggaaacacaacttgttttgatatgggaagcgaattccgattcagatattaggagcacattgtcgtccgaacactcatccatgaagaagttgacggaattgatggatactgatatgtcgggaaatcttcaaaccattgaaagcgctttagaaatagagagagatgaagccttggttaaagaagatatcatcaaagtatggctctgttgccacgaggagaggatgagattcatttattcaaggaaggctgggttgccgttggttctaccatctggactatgcgtactctctctttcctcctgtagtattacagatggagctttagctatttgccttggtgggcttacttcacttagaaatttgttcttaacagagattatgactttaactacgcttccaccggaagaggtcttccaacatttgggcaatcttagatacttggtcattcgttcctgctggtgtctcagatcattcgggggcttgcgatctgctacctctctttcagaaataagattattttcctgtccttctttacagttggcacgtggcgcagaatttatgcaaatgtcccttgagaagctatgtgtatacaactgtgtgctttcagctgacttcttctgcggtgactggccacatctgaataatattgggttatgtgggtgtagaagctctgcgtccttgtatgttggtgatcttacctccctcaaatcattctcactatatcatttgccagatttatgcgtgctggaaggtttgtcttacctacagcttcaccacgtgcatttgatagatgttccaaagcttactaccgagagcatctcacagttccgcgtacagcgttcgctctatattagcagttccgttatgctcaaccacatgatctccgctgaaggtttcaaagttccagggtttctctctcttgaaagctgcaagaagccatccgtttcattcgaagaatctgcaaatttcacatccgtcaagtgcctgaggttatgtaattgtgaaatgaggtcactaccaggaaatatgaagtgcctatccagtctaacgaaactcgatatctatgactgccccaacataacatctctaccagatctgccgtcctccctccagcatatatgtatatggggttgtgagcttttgaagaagagctgcagggcacctgatggagaaagctggccaaagattgcgcatatccgctggaaggaattcagatga</cdnaseq>|
AA = <aaseq>MAEVVLAGLRLAATPICVKLLCNASTCLGVDMTRELHELETIII PQFELVIEAAEKGNHRAKLDRWLRELKQAFYNAEDLLDEHEYNILKCKAKHKDSLVKD STQVHDSSISNILKQPMRAVSSRMSNLRPENRKILCQLNELKTMLEKAKEFRELIHLP AGNSLEGPSVPTIVVPVVTSLLPPRVFGRNMDRDRIIHLLTKPMATVSSSVGYSGLAI VAHGGAGKSTLAQCVYNDKRVQEHFDVRIWVCISRKLDVHRHTREIIESATNGECPRV DNLDTLQCRLKDIMQKSEKFLLVLDDVWFDESVNEREWDQLLDPLVSQQEGSRVLVTS RRDVLPAALHCKDVVHLENMEDAEFLALFKYHAFSGTEIRNPQLHARLEEVAEKIAKR LGQSPLAARTVGSQLSRNKDIAIWKSALNIENLSEPMKALLWSYNKLDSRLQRCFLYC SLFPKGHKYKIDEMVDLWVAEGLVDSRNQGDKRIEDIGRDYFNEMVSGSFFQPVSERY MGTWYIMHDLLHDLAESLTKEDCFRLEDDGVKEIPATVRHLSICVDSMKFHKQKICKL RYLRTVICIDPLMDDGDDIFNQLLKNLKKLRVLHLSFYNSSSLPECIGELKHLRYLSI ISTLISELPRSLCTLFHLELLHLNDKVKNLPDRLCNLRKLRRLEAYDDRNRMYKLYRA ALPQIPYIGKLSLLQDIDGFCVQKQKGYELRQLRDMNKLGGNLRVVNLENVTGKDEAS ESKLHQKTHLRGLHLSWNDVDDMDVSHLEILEGLRPPSQLEDLTIEGYKSTMYPSWLL DGSYFENLESFTLANCCVIGSLPPNTEIFRHCMTLTLENVPNMKTLPFLPEGLTSLSI EGCPLLVFTTNNDELEHHDYRESITRANNLETQLVLIWEANSDSDIRSTLSSEHSSMK KLTELMDTDMSGNLQTIESALEIERDEALVKEDIIKVWLCCHEERMRFIYSRKAGLPL VLPSGLCVLSLSSCSITDGALAICLGGLTSLRNLFLTEIMTLTTLPPEEVFQHLGNLR YLVIRSCWCLRSFGGLRSATSLSEIRLFSCPSLQLARGAEFMQMSLEKLCVYNCVLSA DFFCGDWPHLNNIGLCGCRSSASLYVGDLTSLKSFSLYHLPDLCVLEGLSYLQLHHVH LIDVPKLTTESISQFRVQRSLYISSSVMLNHMISAEGFKVPGFLSLESCKKPSVSFEE SANFTSVKCLRLCNCEMRSLPGNMKCLSSLTKLDIYDCPNITSLPDLPSSLQHICIWG CELLKKSCRAPDGESWPKIAHIRWKEFR</aaseq>|
DNA = <dnaseqindica>4282..8154#gggatctcccgccgttcggagacctaggggagcaagggggagactagagaaggttgcgctccatgaagtcatggtgcaccttaggtatcttgaaaggttttaccaagcgacctggttattgaagcgtggagaagaggctccagtgcggcaggagaaagcagatgagtagaggtacgatctcttcaggagaaagcagagcccagctgcccaggtggtggggagagattatcaaatttttcggtggtggtttggcacgggactctgccgcttcgctgccttcaaggctcaagctcagggtgactggcggcatctgacaggttcatttcctcttgcttatgtcttttagttgcaaatcaattaccattggttgtgggtttgaattattgggcgaaatgctcatgcgttggtcttgaaatttagctttttatctatctctatgttttacattataagtcgctttaatttttttctaattatttttttaagtttgatcaagttcatataaaaatgcaacaacattttcaactcaaaacaaatatattaatataatatattcaatgctatatgtaatgaaactaatttagtgttatgaatgttactatatttttttataaagttggttaaatctaaaaaggtttaattaaaaaaaatcaaaactacttataatacaagtaaaatagagagaattgcatagaagtggaagtgtgtcggtgtctgtactggttctactcacctagtcacctccctgccactgctattctgctaatagtgttagagaatacgcacgcacagataatggcaaggacgtcatttagaagagcccactagtgcgtgtcatgttgcttatgtgcaaattttatacactttcttgactgaccaaaggttgtatgcggtcattttttttttctctcggtgctagctttaatatacggaaagccgtttagagaagatggcatgacaataatggagcatatatatataaactagctcgtaccctgcggcgttgctgcgggtagatattgtaattgcaagtgtccagagaataacaaataaaattttccatggaacaatcatgatggccaaattgtataatatatggctgaaagttcacattatctaaaacaaatatttttcgctgcggtcggagtgtatattaagaaggagagagtttaagcaaactgtacaagagaacaatcagagccaaagctcacatacagaaccaggacactggttcgccacacacacatggttcaaacttcaaacattactgggagtctgatatacatttctttcttgtcttcctgcaaatttgtgttggcactagcttcatacagacctcaactctactgatccaagaacaacatgtagggctacatcctacatgtatacccaaatacccaatacaaacacaaaatagacgaagctaccctgcaatgcaatcagtaataagatcaatagttgataccgaagccaccagccagcatttcgggtaaacatagagcaacaatgaatataagcaagtctcttaagaattacgttctcattttgataaactggcaagcacgggtgaattttgcatcaacacccaacggaaatttgattagcattccttgaaatatgtcactgttgatgaaggtaaaaaagtaaacacaaagtgaagcccatagagcttcagtttagttcaggcatcaacatagtttaactcatgaacctccaaattaaataaattatttatttcattattatatatcataccttggtacatttgtcagcctcacggcagtagattttgcccatgttgcagtaagttacacacacaaacaccaaatatgctagtttttaaactattcttgtttcactagatttaattgcatttggttttttactatgctatttcggagttgtttaatttgatagtgggggccatagagtataatgaaaaacagcaaggtaatgagaaccagagcatagtccggctagaatattttaggagtccttttaatgaggtccagcttgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattctccaatttctccaaattgtaacatggtatcagacccttgttccaaaaaccctagccaccaaatccgatcatcaccggcgcaccggcggcggtgagccgccggcgagccatcatcatcgtcctccttcatccctcaacgtttttgtctccaagcagcaagcctgcagttcgtcgtcgtcaccgtgttcctgttcgccggtcgcagccgccgccagttgcggcgccgcgcccgttcccacttctccacccacgcagccacgccgtcgtggtgttcatccacgctgctcgtcgcggggctcgttcttgccgccgttcgagagccgccgctgtggcctcgtcaatctacatcaaggcatcaagcaacatcagtttttggcccatttcttgtcagttagcatcagcaaaccatcgtcctctctgatctgcagcagcaacaacaacaactcccgttcacctcctagtagatgcagcagctagcccattgttgtctcctaatttcagcagtagaatttccatgttccattgtttgtgttgcaacatgcattctgcagattgtcatcaagcttgtattccaccacatgtgttagtcagtttttgcgcttgctaatcgctgccatccactactcactcatcaatcatcaccatgctttgttattgttagctacttattataatccaattttacttctccaaattatcatgatggcttgtagtagtcactggttaggtcattagctgcttgcttgctactgtcaacatgttcactagcatcctttgtgtcttttgctagtaatgtgcatctctgcttgcagtccatctatcaatattgcctttttgctatatccatcaatccgtgttctttactaatcaaattcctgtgtttcctaccgagtccagcaaaatccattgcatatccttcattttgtcgaagctgtgccagtccatctcttcatggtcatttcatcatcaccagctccccgtcggagctggccttcattgtcacttcatatcttcatcatctccatgtcggagcttgtcatcatcatcatcaatcagtgaaggtccgaagaacagtttgtgaagggccttcatcaatatcaatgtctcatcatcaatcaaaggaccgaagaacagcttgtgaaggtccattgtcatcatcatcaacatctccgaatcaatgaagagccgaagaacgattcgtgaaggcttcaaggtaccgagggccgaagaacagtttgtgaaggctccgaggtccatttcattgaggagggggtcttttcttcttcatcatccaagaattgacacacttcgacgacatcatcaagactttggagataggctaatatttagattgtgttctcatgtttagtttacctttcaaatgcaatgttattacatacacattgcatttgagtgggggtgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattccccaatttctccaaattgtgacacagctcatctgaatattagagaaatataaatttgccactaacatttaacaatgtcccacttgtctactccctccgtcaaaaaaaaccaaacctaagacttagatgtgatataatctagtatctgtctagattcgttgtactagattatggcttaaatactagattaagttttttttgggacggagggagtatgtctaaccaaaataacacaattgatttgcatattgtgttttgcctcgcattgtgccgaagcaatcttatctgtggcaattggtagagttcttcctctcacaccttctctgcaatacaaacgcatctggctctggtaaccttgcactcctctcttagtatttctttccttcacgctttcttgtctctgcttaagttctctatcgtttggtaccatcatgcattaattcctttttcctctatttcccactccaaacatttccataaccactcacatacacaaaaataaaaaaccagacagagagaaaaaaacttgttttgggagttctgttgttatcttcctcacagaagaaaaatctctccatcaccatctgttcttatgccctgcttctattcattcacttgttcacagctgcagtagtgctgttccatggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaatgtgtttacaatgacaagagggtacaagaacattttgacgtcaggatatgggtctgcatctcacgcaaacttgatgttcatcgccatacacgtgagattatcgagtcagcaacaaatggagagtgccctcgtgtggacaatcttgatactctccagtgcagattgaaggacataatgcaaaaatcagagaaattcctgcttgtgttggatgacgtctggtttgatgaatccgtcaatgagagggaatgggaccagttgcttgatcctcttgtttctcagcaggaggggagcagagttttggtgacttctagacgagatgtacttccagctgctcttcactgtaaggatgttgttcatttggaaaacatggaagatgccgagttcttggctctattcaaataccatgctttctctggaacagaaatcagaaatccacagttgcatgcacggctagaagaggtcgcagagaagattgctaaaaggttaggacaatcgcctttagcagcaaggacagtaggctcccagctgagcaggaataaggatattgccatatggaaaagtgctctaaatattgaaaacttaagtgagcccatgaaagctctgttgtggagttataataaattagattcacgtctgcagagatgttttctgtactgcagcttatttccaaaaggtcacaagtataaaattgatgagatggttgacctttgggtggcagaggggctagttgattcacgcaaccagggggataagagaattgaagatattggcagggattacttcaatgagatggtgtcaggatctttctttcaaccagtttctgaaagatatatgggtacatggtacattatgcatgatctgcttcatgatttggcagagtcactgactaaagaagactgcttcagattagaagatgatggggtgaaagagataccagccactgttcgacatctatctatttgtgttgacagtatgaaattccataagcaaaagatctgcaagctacgttatttacgcactgttatctgcattgaccctctcatggatgacggagatgatatttttaatcagctattgaagaatctgaagaagctacgtgtactacatttgtcgttttacaacagtagcagcttgcctgaatgtattggtgagctgaagcaccttcgatatttgagtatcatcagcacattgatttctgaactgccaagatcattgtgtactcttttccacttggagctacttcatttgaatgacaaggtcaagaatttgccggacagactctgtaatttaagaaagttacgacgtcttgaagcatatgatgacagaaacagaatgtataaattgtatagagcagctctccctcaaattccttacataggcaagctatctttgctgcaagatattgatggattttgcgtgcaaaagcagaagggatatgagttgcgacaactgagggacatgaacaagctcggtggtaatttgcgtgtcgttaatcttgaaaatgtaactggaaaggatgaagcctcagagtcgaagttgcatcagaaaactcatcttagaggtttgcatctctcctggaatgatgtggatgacatggatgtttcacatttggagattctagaaggtttgaggccgccatctcaattggaggaccttacaatcgaaggttacaaatccaccatgtacccaagctggttacttgatggatcctattttgagaatctagagtcttttacgcttgctaattgctgtgtgataggaagcctaccacccaataccgagatctttaggcactgtatgacacttacccttgagaatgtcccgaatatgaagacactaccttttcttccagaaggtctcacaagcctatcaattgaggggtgcccactgcttgtgttcactactaataacgatgaactggaacaccatgattatagggagagcatcacgagggcaaacaacctggaaacacaacttgttttgatatgggaagcgaattccgattcagatattaggagcacattgtcgtccgaacactcatccatgaagaagttgacggaattgatggatactgatatgtcgggaaatcttcaaaccattgaaagcgctttagaaatagagagagatgaagccttggttaaagaagatatcatcaaagtatggctctgttgccacgaggagaggatgagattcatttattcaaggaaggctgggttgccgttggttctaccatctggactatgcgtactctctctttcctcctgtagtattacagatggagctttagctatttgccttggtgggcttacttcacttagaaatttgttcttaacagagattatgactttaactacgcttccaccggaagaggtcttccaacatttgggcaatcttagatacttggtcattcgttcctgctggtgtctcagatcattcgggggcttgcgatctgctacctctctttcagaaataagattattttcctgtccttctttacagttggcacgtggcgcagaatttatgcaaatgtcccttgagaagctatgtgtatacaactgtgtgctttcagctgacttcttctgcggtgactggccacatctgaataatattgggttatgtgggtgtagaagctctgcgtccttgtatgttggtgatcttacctccctcaaatcattctcactatatcatttgccagatttatgcgtgctggaaggtttgtcttacctacagcttcaccacgtgcatttgatagatgttccaaagcttactaccgagagcatctcacagttccgcgtacagcgttcgctctatattagcagttccgttatgctcaaccacatgatctccgctgaaggtttcaaagttccagggtttctctctcttgaaagctgcaagaagccatccgtttcattcgaagaatctgcaaatttcacatccgtcaagtgcctgaggttatgtaattgtgaaatgaggtcactaccaggaaatatgaagtgcctatccagtctaacgaaactcgatatctatgactgccccaacataacatctctaccagatctgccgtcctccctccagcatatatgtatatggggttgtgagcttttgaagaagagctgcagggcacctgatggagaaagctggccaaagattgcgcatatccgctggaaggaattcagatgaattgtgcttcagaatcactactacggagaagcgcaaaggtgaaggtcccatcaatgcattatcagaagtactctatgtaatatttatttcattcgctcatgatcttacgaacacatcattaacttcacgtttcctttgttttgcagacaccccaagatttgctctctcaggtaacttcgtctcttcgccacctgctacaaggtagagcgcctcatgctcatatagcagtttcagctctctcctgtagtcctgtggtctgtccggttgtgctgtccactctgttccctgcttcgtcgattgccacatttacgtttccctgctgccttctaccgacccacgcctcccttcatccggtctctgccactgacggcccatgatcgtgtccttttgctttcaccccttaaatggagtcttgttggtccaagaaaatctctgtttgtctgttgaacactcaactatctctcgtattttgtgtgatatcgctcgtatttgattgcgtgatgtgataatatattgaagtaattggattttgtacttcgcac</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185663.1 RefSeq:Os01g0782100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

Os01g0782100

2014-05-28T02:59:07Z

Liangdan2013: /* Function */

Please input one-sentence summary here.
This rice blast resistance genes was found by two Japanese scholars Imbe and Matsumoto in 1985, they called this gene Pi-sh,this gene has the moderate resistance to the blast fungus strain Kyu77-07A in rice variety Shin-2.this gene has been characterized by using the method of classical genetic.And this gene is linking to pi-t.
==Annotated Information==
===Function===
The genes are accounting for the moderate resistance of the rice variety Shin 2 and some other varieties to the blast fungus strain Kyu 77-07A. Aichi Asahi (Pi-a) and carried out in the present study. Yashiro-mochi (Pi-ta), were found to be susceptible to the strain, while Ishikari Shirol*.e (Pi-i), Kanto 51 (Pi-k), Tsuyuake (Pi-k'!b), Fukunishiki (Pi-z) and Toride I (Pi-zt) were resistant, and Shin 2 (Pi-ks) and Pi N0.4 (Pi-ta2) moderately resistant. Therefore, Kyu 77-07A was classified as one of the strains belonging to race 102. Howver, the variety Reiho with the resistance gene Pi-ta2 was susceptible to this strain. Based on the knovn facts mentioned above, the objectives 0L the present study were' to determinA- whether Pi-ks is the gene responsible for the moderate resistance of Shin 2' to Kyu 77-07A along with the reason for the different reactions to Kyu 77-07A betweerL Pi N0.4 and Reiho which both have the same resistance gene Pi-ta2. For the gene analysis, the F2 or F3 plants of the crosses were inoculated with Kyu 77-07A and three other fungus strains using the spraying rnethod when the plants were at the four- to five-1eaf stage. Two varieties, Mineyutaka. and Saikai 155 belonging to the Shin 2 type varieties, were used as the representative parents of the varieties susceptible to Kyu 77-07A for the crosses( Y. Koide,2010).

===Expression===
To identify novel factors required for R gene-mediated resistance in rice, we used rice insertional mutant lines, induced by the endogenous retrotransposon Tos17, in a forward screen involving the rice blast fungus Magnaporthe oryzae. We inoculated 41,119 mutant lines with the fungus using a high throughput procedure, and identified 86 mutant lines with diminished resistance. A genome analysis revealed that 72 of the 86 lines contained mutations in a gene encoding a nucleotide binding site (NB) and leucine rich repeat (LRR) domain-containing (NLR) protein. A genetic complementation analysis and a pathogenesis assay demonstrated that this NLR gene encodes Pish, which confers resistance against races of M. oryzae containing avrPish. The other 14 lines have intact copies of the Pish gene, suggesting that they may contain mutations in the signaling components downstream of Pish. The genome analysis indicated that Pish and its neighboring three NLR genes are high similar to one another and are tandemly located. An in silico analysis of a Tos17 flanking sequence database revealed that this region is a "hot spot" for insertion. Intriguingly, the insertion sites are not distributed evenly among these four NLR genes, despite their similarity at the sequence and expression levels.
In this work we isolated the R gene Pish, and identified several other mutants involved in the signal transduction required for Pish-mediated resistance. These results indicate that our genetic approach is efficient and useful for unveiling novel aspects of defense signaling in rice. Furthermore, our data provide experimental evidence that R gene clusters have the potential to be highly preferred targets for transposable element insertions in plant genomes. Based on this finding, a possible mechanism underlying the high variability of R genes is discussed.
Primer pairs utilized in this work(Akira Takahashi,2010).

[[File:sequence.jpg]]

===Evolution===
A study was conducted to investigate the relationship between phenotype of blast resistance and resistance gene analog polymorphism of rice, and search molecule hereditary basis of broad spectrum and durable resistance.
【Method】 Comparison of clustering analysis was investigated using spectrum of resistance to blast and polymorphism of resistance gene analog (RGA) in 25 varieties for blast resistance identification and 20 varieties lines).
【Result】 The resistance spectrum clustering analysis showed that the 45 varieties (lines) could be divided into group A and group B with the genetic similarity coefficient of 0.450. Group A and group B could be divided into subclassⅰ, subclass , ⅱ subclass , ⅲ subclass ⅳ, respectively, with 0.618 genetic similarity coefficient. The RGA-PCR clustering analysis showed that proposed the 45 varieties (lines) could be divided into group Ⅰ and group Ⅱ which clearly inclined the Indica-japonica differentiation with 0.620 genetic similarity coefficient. Group Ⅰ could be divided into six subclasses and group Ⅱ could be divided into seven subclasses with 0.783 genetic similarity coefficient. The resistance spectrum clustering analysis showed that some varieties with similar resistance spectrum could finely fall into the same group, while the RGA-PCR clustering analysis showed that some varieties with the similar genetic background could fall into the same group. For some varieties with low resistance frequency or high resistance frequency, there was a better corresponding relationship between the resistance spectrum clustering and the RGA-PCR clustering. General comparison of clustering analysis showed that here was no parallelism relationship between group and group in two different types of the clustering.
【Conclusion】 It could more accurately reflect their enetic background to test resistance to single strain and analysis on RGA polymorphism for resistance parents, and avoid applying the same source of resistance gain and again, and enrich rice resistance germplasm, and breed durable resistance varieties.

You can also add sub-section(s) at will.

==Labs working on this gene==
1.Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Ibaraki, 305-8602，Japan.
2.International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines;
3.Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, Japan;
4.Indonesian Center for Rice Research (ICRR), JL. Raya Muara No. 25A Ciapus Bogor, Subang, West Java, Indonesia;
5.Agricultural Genetics Institute,Conhue, Tuliem, Hanoi, Vietnam;
6.Faculty of Agricultural Sciences, University of Burundi, BP 2940 Bujumbura, Burundi;

==References==
1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta
Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.)
Plant Breeding, 2010, 129(6): 670-675
2. Akira Takahashi;Nagao Hayashi;Akio Miyao;Hirohiko Hirochika
Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging
BMC Plant Biology, 2010, 10: 175
3. Tokio IMBE;Shohei MATSUMOTO
Inheritance of Resrstance of Rice Vanetles to the Blast Fungus Strains Virulent to the Variety "Reiho"
Japanese Journal of Breeding, 1985, 35(0): 332-339.
4.鄂志国, 张丽靖, 焦桂爱, 等. 稻瘟病抗性基因的鉴定及利用进展[J]. 中国水稻科学, 2008, 22(5): 533-540.
5.杨勤忠, 林菲, 冯淑杰, 等. 水稻稻瘟病抗性基因的分子定位及克隆研究进展[J]. 中国农业科学, 2009, 42(5): 1601-1615.
6.任鄄胜, 肖培村, 陈勇, 等. 水稻品种稻瘟病抗性和抗病基因同源序列多态性分析[J]. 中国农业科学, 2009, 42(1): 1-9.
==Structured Information==
{{JaponicaGene|
GeneName = Os01g0782100|
Description = Similar to NBS-LRR protein (Fragment)|
Version = NM_001185663.1 GI:297720460 GeneID:9271927|
Length = 8696 bp|
Definition = Oryza sativa Japonica Group Os01g0782100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:34892692..34901387|
CDS = 34896973..34900845|
GCID = <gbrowseImage1>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaatgtgtttacaatgacaagagggtacaagaacattttgacgtcaggatatgggtctgcatctcacgcaaacttgatgttcatcgccatacacgtgagattatcgagtcagcaacaaatggagagtgccctcgtgtggacaatcttgatactctccagtgcagattgaaggacataatgcaaaaatcagagaaattcctgcttgtgttggatgacgtctggtttgatgaatccgtcaatgagagggaatgggaccagttgcttgatcctcttgtttctcagcaggaggggagcagagttttggtgacttctagacgagatgtacttccagctgctcttcactgtaaggatgttgttcatttggaaaacatggaagatgccgagttcttggctctattcaaataccatgctttctctggaacagaaatcagaaatccacagttgcatgcacggctagaagaggtcgcagagaagattgctaaaaggttaggacaatcgcctttagcagcaaggacagtaggctcccagctgagcaggaataaggatattgccatatggaaaagtgctctaaatattgaaaacttaagtgagcccatgaaagctctgttgtggagttataataaattagattcacgtctgcagagatgttttctgtactgcagcttatttccaaaaggtcacaagtataaaattgatgagatggttgacctttgggtggcagaggggctagttgattcacgcaaccagggggataagagaattgaagatattggcagggattacttcaatgagatggtgtcaggatctttctttcaaccagtttctgaaagatatatgggtacatggtacattatgcatgatctgcttcatgatttggcagagtcactgactaaagaagactgcttcagattagaagatgatggggtgaaagagataccagccactgttcgacatctatctatttgtgttgacagtatgaaattccataagcaaaagatctgcaagctacgttatttacgcactgttatctgcattgaccctctcatggatgacggagatgatatttttaatcagctattgaagaatctgaagaagctacgtgtactacatttgtcgttttacaacagtagcagcttgcctgaatgtattggtgagctgaagcaccttcgatatttgagtatcatcagcacattgatttctgaactgccaagatcattgtgtactcttttccacttggagctacttcatttgaatgacaaggtcaagaatttgccggacagactctgtaatttaagaaagttacgacgtcttgaagcatatgatgacagaaacagaatgtataaattgtatagagcagctctccctcaaattccttacataggcaagctatctttgctgcaagatattgatggattttgcgtgcaaaagcagaagggatatgagttgcgacaactgagggacatgaacaagctcggtggtaatttgcgtgtcgttaatcttgaaaatgtaactggaaaggatgaagcctcagagtcgaagttgcatcagaaaactcatcttagaggtttgcatctctcctggaatgatgtggatgacatggatgtttcacatttggagattctagaaggtttgaggccgccatctcaattggaggaccttacaatcgaaggttacaaatccaccatgtacccaagctggttacttgatggatcctattttgagaatctagagtcttttacgcttgctaattgctgtgtgataggaagcctaccacccaataccgagatctttaggcactgtatgacacttacccttgagaatgtcccgaatatgaagacactaccttttcttccagaaggtctcacaagcctatcaattgaggggtgcccactgcttgtgttcactactaataacgatgaactggaacaccatgattatagggagagcatcacgagggcaaacaacctggaaacacaacttgttttgatatgggaagcgaattccgattcagatattaggagcacattgtcgtccgaacactcatccatgaagaagttgacggaattgatggatactgatatgtcgggaaatcttcaaaccattgaaagcgctttagaaatagagagagatgaagccttggttaaagaagatatcatcaaagtatggctctgttgccacgaggagaggatgagattcatttattcaaggaaggctgggttgccgttggttctaccatctggactatgcgtactctctctttcctcctgtagtattacagatggagctttagctatttgccttggtgggcttacttcacttagaaatttgttcttaacagagattatgactttaactacgcttccaccggaagaggtcttccaacatttgggcaatcttagatacttggtcattcgttcctgctggtgtctcagatcattcgggggcttgcgatctgctacctctctttcagaaataagattattttcctgtccttctttacagttggcacgtggcgcagaatttatgcaaatgtcccttgagaagctatgtgtatacaactgtgtgctttcagctgacttcttctgcggtgactggccacatctgaataatattgggttatgtgggtgtagaagctctgcgtccttgtatgttggtgatcttacctccctcaaatcattctcactatatcatttgccagatttatgcgtgctggaaggtttgtcttacctacagcttcaccacgtgcatttgatagatgttccaaagcttactaccgagagcatctcacagttccgcgtacagcgttcgctctatattagcagttccgttatgctcaaccacatgatctccgctgaaggtttcaaagttccagggtttctctctcttgaaagctgcaagaagccatccgtttcattcgaagaatctgcaaatttcacatccgtcaagtgcctgaggttatgtaattgtgaaatgaggtcactaccaggaaatatgaagtgcctatccagtctaacgaaactcgatatctatgactgccccaacataacatctctaccagatctgccgtcctccctccagcatatatgtatatggggttgtgagcttttgaagaagagctgcagggcacctgatggagaaagctggccaaagattgcgcatatccgctggaaggaattcagatga</cdnaseq>|
AA = <aaseq>MAEVVLAGLRLAATPICVKLLCNASTCLGVDMTRELHELETIII PQFELVIEAAEKGNHRAKLDRWLRELKQAFYNAEDLLDEHEYNILKCKAKHKDSLVKD STQVHDSSISNILKQPMRAVSSRMSNLRPENRKILCQLNELKTMLEKAKEFRELIHLP AGNSLEGPSVPTIVVPVVTSLLPPRVFGRNMDRDRIIHLLTKPMATVSSSVGYSGLAI VAHGGAGKSTLAQCVYNDKRVQEHFDVRIWVCISRKLDVHRHTREIIESATNGECPRV DNLDTLQCRLKDIMQKSEKFLLVLDDVWFDESVNEREWDQLLDPLVSQQEGSRVLVTS RRDVLPAALHCKDVVHLENMEDAEFLALFKYHAFSGTEIRNPQLHARLEEVAEKIAKR LGQSPLAARTVGSQLSRNKDIAIWKSALNIENLSEPMKALLWSYNKLDSRLQRCFLYC SLFPKGHKYKIDEMVDLWVAEGLVDSRNQGDKRIEDIGRDYFNEMVSGSFFQPVSERY MGTWYIMHDLLHDLAESLTKEDCFRLEDDGVKEIPATVRHLSICVDSMKFHKQKICKL RYLRTVICIDPLMDDGDDIFNQLLKNLKKLRVLHLSFYNSSSLPECIGELKHLRYLSI ISTLISELPRSLCTLFHLELLHLNDKVKNLPDRLCNLRKLRRLEAYDDRNRMYKLYRA ALPQIPYIGKLSLLQDIDGFCVQKQKGYELRQLRDMNKLGGNLRVVNLENVTGKDEAS ESKLHQKTHLRGLHLSWNDVDDMDVSHLEILEGLRPPSQLEDLTIEGYKSTMYPSWLL DGSYFENLESFTLANCCVIGSLPPNTEIFRHCMTLTLENVPNMKTLPFLPEGLTSLSI EGCPLLVFTTNNDELEHHDYRESITRANNLETQLVLIWEANSDSDIRSTLSSEHSSMK KLTELMDTDMSGNLQTIESALEIERDEALVKEDIIKVWLCCHEERMRFIYSRKAGLPL VLPSGLCVLSLSSCSITDGALAICLGGLTSLRNLFLTEIMTLTTLPPEEVFQHLGNLR YLVIRSCWCLRSFGGLRSATSLSEIRLFSCPSLQLARGAEFMQMSLEKLCVYNCVLSA DFFCGDWPHLNNIGLCGCRSSASLYVGDLTSLKSFSLYHLPDLCVLEGLSYLQLHHVH LIDVPKLTTESISQFRVQRSLYISSSVMLNHMISAEGFKVPGFLSLESCKKPSVSFEE SANFTSVKCLRLCNCEMRSLPGNMKCLSSLTKLDIYDCPNITSLPDLPSSLQHICIWG CELLKKSCRAPDGESWPKIAHIRWKEFR</aaseq>|
DNA = <dnaseqindica>4282..8154#gggatctcccgccgttcggagacctaggggagcaagggggagactagagaaggttgcgctccatgaagtcatggtgcaccttaggtatcttgaaaggttttaccaagcgacctggttattgaagcgtggagaagaggctccagtgcggcaggagaaagcagatgagtagaggtacgatctcttcaggagaaagcagagcccagctgcccaggtggtggggagagattatcaaatttttcggtggtggtttggcacgggactctgccgcttcgctgccttcaaggctcaagctcagggtgactggcggcatctgacaggttcatttcctcttgcttatgtcttttagttgcaaatcaattaccattggttgtgggtttgaattattgggcgaaatgctcatgcgttggtcttgaaatttagctttttatctatctctatgttttacattataagtcgctttaatttttttctaattatttttttaagtttgatcaagttcatataaaaatgcaacaacattttcaactcaaaacaaatatattaatataatatattcaatgctatatgtaatgaaactaatttagtgttatgaatgttactatatttttttataaagttggttaaatctaaaaaggtttaattaaaaaaaatcaaaactacttataatacaagtaaaatagagagaattgcatagaagtggaagtgtgtcggtgtctgtactggttctactcacctagtcacctccctgccactgctattctgctaatagtgttagagaatacgcacgcacagataatggcaaggacgtcatttagaagagcccactagtgcgtgtcatgttgcttatgtgcaaattttatacactttcttgactgaccaaaggttgtatgcggtcattttttttttctctcggtgctagctttaatatacggaaagccgtttagagaagatggcatgacaataatggagcatatatatataaactagctcgtaccctgcggcgttgctgcgggtagatattgtaattgcaagtgtccagagaataacaaataaaattttccatggaacaatcatgatggccaaattgtataatatatggctgaaagttcacattatctaaaacaaatatttttcgctgcggtcggagtgtatattaagaaggagagagtttaagcaaactgtacaagagaacaatcagagccaaagctcacatacagaaccaggacactggttcgccacacacacatggttcaaacttcaaacattactgggagtctgatatacatttctttcttgtcttcctgcaaatttgtgttggcactagcttcatacagacctcaactctactgatccaagaacaacatgtagggctacatcctacatgtatacccaaatacccaatacaaacacaaaatagacgaagctaccctgcaatgcaatcagtaataagatcaatagttgataccgaagccaccagccagcatttcgggtaaacatagagcaacaatgaatataagcaagtctcttaagaattacgttctcattttgataaactggcaagcacgggtgaattttgcatcaacacccaacggaaatttgattagcattccttgaaatatgtcactgttgatgaaggtaaaaaagtaaacacaaagtgaagcccatagagcttcagtttagttcaggcatcaacatagtttaactcatgaacctccaaattaaataaattatttatttcattattatatatcataccttggtacatttgtcagcctcacggcagtagattttgcccatgttgcagtaagttacacacacaaacaccaaatatgctagtttttaaactattcttgtttcactagatttaattgcatttggttttttactatgctatttcggagttgtttaatttgatagtgggggccatagagtataatgaaaaacagcaaggtaatgagaaccagagcatagtccggctagaatattttaggagtccttttaatgaggtccagcttgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattctccaatttctccaaattgtaacatggtatcagacccttgttccaaaaaccctagccaccaaatccgatcatcaccggcgcaccggcggcggtgagccgccggcgagccatcatcatcgtcctccttcatccctcaacgtttttgtctccaagcagcaagcctgcagttcgtcgtcgtcaccgtgttcctgttcgccggtcgcagccgccgccagttgcggcgccgcgcccgttcccacttctccacccacgcagccacgccgtcgtggtgttcatccacgctgctcgtcgcggggctcgttcttgccgccgttcgagagccgccgctgtggcctcgtcaatctacatcaaggcatcaagcaacatcagtttttggcccatttcttgtcagttagcatcagcaaaccatcgtcctctctgatctgcagcagcaacaacaacaactcccgttcacctcctagtagatgcagcagctagcccattgttgtctcctaatttcagcagtagaatttccatgttccattgtttgtgttgcaacatgcattctgcagattgtcatcaagcttgtattccaccacatgtgttagtcagtttttgcgcttgctaatcgctgccatccactactcactcatcaatcatcaccatgctttgttattgttagctacttattataatccaattttacttctccaaattatcatgatggcttgtagtagtcactggttaggtcattagctgcttgcttgctactgtcaacatgttcactagcatcctttgtgtcttttgctagtaatgtgcatctctgcttgcagtccatctatcaatattgcctttttgctatatccatcaatccgtgttctttactaatcaaattcctgtgtttcctaccgagtccagcaaaatccattgcatatccttcattttgtcgaagctgtgccagtccatctcttcatggtcatttcatcatcaccagctccccgtcggagctggccttcattgtcacttcatatcttcatcatctccatgtcggagcttgtcatcatcatcatcaatcagtgaaggtccgaagaacagtttgtgaagggccttcatcaatatcaatgtctcatcatcaatcaaaggaccgaagaacagcttgtgaaggtccattgtcatcatcatcaacatctccgaatcaatgaagagccgaagaacgattcgtgaaggcttcaaggtaccgagggccgaagaacagtttgtgaaggctccgaggtccatttcattgaggagggggtcttttcttcttcatcatccaagaattgacacacttcgacgacatcatcaagactttggagataggctaatatttagattgtgttctcatgtttagtttacctttcaaatgcaatgttattacatacacattgcatttgagtgggggtgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattccccaatttctccaaattgtgacacagctcatctgaatattagagaaatataaatttgccactaacatttaacaatgtcccacttgtctactccctccgtcaaaaaaaaccaaacctaagacttagatgtgatataatctagtatctgtctagattcgttgtactagattatggcttaaatactagattaagttttttttgggacggagggagtatgtctaaccaaaataacacaattgatttgcatattgtgttttgcctcgcattgtgccgaagcaatcttatctgtggcaattggtagagttcttcctctcacaccttctctgcaatacaaacgcatctggctctggtaaccttgcactcctctcttagtatttctttccttcacgctttcttgtctctgcttaagttctctatcgtttggtaccatcatgcattaattcctttttcctctatttcccactccaaacatttccataaccactcacatacacaaaaataaaaaaccagacagagagaaaaaaacttgttttgggagttctgttgttatcttcctcacagaagaaaaatctctccatcaccatctgttcttatgccctgcttctattcattcacttgttcacagctgcagtagtgctgttccatggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaat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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185663.1 RefSeq:Os01g0782100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

Os01g0782100

2014-05-28T02:58:16Z

Liangdan2013: /* Expression */

Please input one-sentence summary here.
This rice blast resistance genes was found by two Japanese scholars Imbe and Matsumoto in 1985, they called this gene Pi-sh,this gene has the moderate resistance to the blast fungus strain Kyu77-07A in rice variety Shin-2.this gene has been characterized by using the method of classical genetic.And this gene is linking to pi-t.
==Annotated Information==
===Function===
The genes are accounting for the moderate resistance of the rice variety Shin 2 and some other varieties to the blast fungus strain Kyu 77-07A. Aichi Asahi (Pi-a) and carried out in the present study. Yashiro-mochi (Pi-ta), were found to be susceptible to the strain, while Ishikari Shirol*.e (Pi-i), Kanto 51 (Pi-k), Tsuyuake (Pi-k'!b), Fukunishiki (Pi-z) and Toride I (Pi-zt) were resistant, and Shin 2 (Pi-ks) and Pi N0.4 (Pi-ta2) moderately resistant. Therefore, Kyu 77-07A was classified as one of the strains belonging to race 102. Howver, the variety Reiho with the resistance gene Pi-ta2 was susceptible to this strain. Based on the knovn facts mentioned above, the objectives 0L the present study were' to determinA- whether Pi-ks is the gene responsible for the moderate resistance of Shin 2' to Kyu 77-07A along with the reason for the different reactions to Kyu 77-07A betweerL Pi N0.4 and Reiho which both have the same resistance gene Pi-ta2. For the gene analysis, the F2 or F3 plants of the crosses were inoculated with Kyu 77-07A and three other fungus strains using the spraying rnethod when the plants were at the four- to five-1eaf stage. Two varieties, Mineyutaka. and Saikai 155 belonging to the Shin 2 type varieties, were used as the representative parents of the varieties susceptible to Kyu 77-07A for the crosses.

===Expression===
To identify novel factors required for R gene-mediated resistance in rice, we used rice insertional mutant lines, induced by the endogenous retrotransposon Tos17, in a forward screen involving the rice blast fungus Magnaporthe oryzae. We inoculated 41,119 mutant lines with the fungus using a high throughput procedure, and identified 86 mutant lines with diminished resistance. A genome analysis revealed that 72 of the 86 lines contained mutations in a gene encoding a nucleotide binding site (NB) and leucine rich repeat (LRR) domain-containing (NLR) protein. A genetic complementation analysis and a pathogenesis assay demonstrated that this NLR gene encodes Pish, which confers resistance against races of M. oryzae containing avrPish. The other 14 lines have intact copies of the Pish gene, suggesting that they may contain mutations in the signaling components downstream of Pish. The genome analysis indicated that Pish and its neighboring three NLR genes are high similar to one another and are tandemly located. An in silico analysis of a Tos17 flanking sequence database revealed that this region is a "hot spot" for insertion. Intriguingly, the insertion sites are not distributed evenly among these four NLR genes, despite their similarity at the sequence and expression levels.
In this work we isolated the R gene Pish, and identified several other mutants involved in the signal transduction required for Pish-mediated resistance. These results indicate that our genetic approach is efficient and useful for unveiling novel aspects of defense signaling in rice. Furthermore, our data provide experimental evidence that R gene clusters have the potential to be highly preferred targets for transposable element insertions in plant genomes. Based on this finding, a possible mechanism underlying the high variability of R genes is discussed.
Primer pairs utilized in this work(Akira Takahashi,2010).

[[File:sequence.jpg]]

===Evolution===
A study was conducted to investigate the relationship between phenotype of blast resistance and resistance gene analog polymorphism of rice, and search molecule hereditary basis of broad spectrum and durable resistance.
【Method】 Comparison of clustering analysis was investigated using spectrum of resistance to blast and polymorphism of resistance gene analog (RGA) in 25 varieties for blast resistance identification and 20 varieties lines).
【Result】 The resistance spectrum clustering analysis showed that the 45 varieties (lines) could be divided into group A and group B with the genetic similarity coefficient of 0.450. Group A and group B could be divided into subclassⅰ, subclass , ⅱ subclass , ⅲ subclass ⅳ, respectively, with 0.618 genetic similarity coefficient. The RGA-PCR clustering analysis showed that proposed the 45 varieties (lines) could be divided into group Ⅰ and group Ⅱ which clearly inclined the Indica-japonica differentiation with 0.620 genetic similarity coefficient. Group Ⅰ could be divided into six subclasses and group Ⅱ could be divided into seven subclasses with 0.783 genetic similarity coefficient. The resistance spectrum clustering analysis showed that some varieties with similar resistance spectrum could finely fall into the same group, while the RGA-PCR clustering analysis showed that some varieties with the similar genetic background could fall into the same group. For some varieties with low resistance frequency or high resistance frequency, there was a better corresponding relationship between the resistance spectrum clustering and the RGA-PCR clustering. General comparison of clustering analysis showed that here was no parallelism relationship between group and group in two different types of the clustering.
【Conclusion】 It could more accurately reflect their enetic background to test resistance to single strain and analysis on RGA polymorphism for resistance parents, and avoid applying the same source of resistance gain and again, and enrich rice resistance germplasm, and breed durable resistance varieties.

You can also add sub-section(s) at will.

==Labs working on this gene==
1.Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Ibaraki, 305-8602，Japan.
2.International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines;
3.Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, Japan;
4.Indonesian Center for Rice Research (ICRR), JL. Raya Muara No. 25A Ciapus Bogor, Subang, West Java, Indonesia;
5.Agricultural Genetics Institute,Conhue, Tuliem, Hanoi, Vietnam;
6.Faculty of Agricultural Sciences, University of Burundi, BP 2940 Bujumbura, Burundi;

==References==
1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta
Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.)
Plant Breeding, 2010, 129(6): 670-675
2. Akira Takahashi;Nagao Hayashi;Akio Miyao;Hirohiko Hirochika
Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging
BMC Plant Biology, 2010, 10: 175
3. Tokio IMBE;Shohei MATSUMOTO
Inheritance of Resrstance of Rice Vanetles to the Blast Fungus Strains Virulent to the Variety "Reiho"
Japanese Journal of Breeding, 1985, 35(0): 332-339.
4.鄂志国, 张丽靖, 焦桂爱, 等. 稻瘟病抗性基因的鉴定及利用进展[J]. 中国水稻科学, 2008, 22(5): 533-540.
5.杨勤忠, 林菲, 冯淑杰, 等. 水稻稻瘟病抗性基因的分子定位及克隆研究进展[J]. 中国农业科学, 2009, 42(5): 1601-1615.
6.任鄄胜, 肖培村, 陈勇, 等. 水稻品种稻瘟病抗性和抗病基因同源序列多态性分析[J]. 中国农业科学, 2009, 42(1): 1-9.
==Structured Information==
{{JaponicaGene|
GeneName = Os01g0782100|
Description = Similar to NBS-LRR protein (Fragment)|
Version = NM_001185663.1 GI:297720460 GeneID:9271927|
Length = 8696 bp|
Definition = Oryza sativa Japonica Group Os01g0782100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:34892692..34901387|
CDS = 34896973..34900845|
GCID = <gbrowseImage1>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaatgtgtttacaatgacaagagggtacaagaacattttgacgtcaggatatgggtctgcatctcacgcaaacttgatgttcatcgccatacacgtgagattatcgagtcagcaacaaatggagagtgccctcgtgtggacaatcttgatactctccagtgcagattgaaggacataatgcaaaaatcagagaaattcctgcttgtgttggatgacgtctggtttgatgaatccgtcaatgagagggaatgggaccagttgcttgatcctcttgtttctcagcaggaggggagcagagttttggtgacttctagacgagatgtacttccagctgctcttcactgtaaggatgttgttcatttggaaaacatggaagatgccgagttcttggctctattcaaataccatgctttctctggaacagaaatcagaaatccacagttgcatgcacggctagaagaggtcgcagagaagattgctaaaaggttaggacaatcgcctttagcagcaaggacagtaggctcccagctgagcaggaataaggatattgccatatggaaaagtgctctaaatattgaaaacttaagtgagcccatgaaagctctgttgtggagttataataaattagattcacgtctgcagagatgttttctgtactgcagcttatttccaaaaggtcacaagtataaaattgatgagatggttgacctttgggtggcagaggggctagttgattcacgcaaccagggggataagagaattgaagatattggcagggattacttcaatgagatggtgtcaggatctttctttcaaccagtttctgaaagatatatgggtacatggtacattatgcatgatctgcttcatgatttggcagagtcactgactaaagaagactgcttcagattagaagatgatggggtgaaagagataccagccactgttcgacatctatctatttgtgttgacagtatgaaattccataagcaaaagatctgcaagctacgttatttacgcactgttatctgcattgaccctctcatggatgacggagatgatatttttaatcagctattgaagaatctgaagaagctacgtgtactacatttgtcgttttacaacagtagcagcttgcctgaatgtattggtgagctgaagcaccttcgatatttgagtatcatcagcacattgatttctgaactgccaagatcattgtgtactcttttccacttggagctacttcatttgaatgacaaggtcaagaatttgccggacagactctgtaatttaagaaagttacgacgtcttgaagcatatgatgacagaaacagaatgtataaattgtatagagcagctctccctcaaattccttacataggcaagctatctttgctgcaagatattgatggattttgcgtgcaaaagcagaagggatatgagttgcgacaactgagggacatgaacaagctcggtggtaatttgcgtgtcgttaatcttgaaaatgtaactggaaaggatgaagcctcagagtcgaagttgcatcagaaaactcatcttagaggtttgcatctctcctggaatgatgtggatgacatggatgtttcacatttggagattctagaaggtttgaggccgccatctcaattggaggaccttacaatcgaaggttacaaatccaccatgtacccaagctggttacttgatggatcctattttgagaatctagagtcttttacgcttgctaattgctgtgtgataggaagcctaccacccaataccgagatctttaggcactgtatgacacttacccttgagaatgtcccgaatatgaagacactaccttttcttccagaaggtctcacaagcctatcaattgaggggtgcccactgcttgtgttcactactaataacgatgaactggaacaccatgattatagggagagcatcacgagggcaaacaacctggaaacacaacttgttttgatatgggaagcgaattccgattcagatattaggagcacattgtcgtccgaacactcatccatgaagaagttgacggaattgatggatactgatatgtcgggaaatcttcaaaccattgaaagcgctttagaaatagagagagatgaagccttggttaaagaagatatcatcaaagtatggctctgttgccacgaggagaggatgagattcatttattcaaggaaggctgggttgccgttggttctaccatctggactatgcgtactctctctttcctcctgtagtattacagatggagctttagctatttgccttggtgggcttacttcacttagaaatttgttcttaacagagattatgactttaactacgcttccaccggaagaggtcttccaacatttgggcaatcttagatacttggtcattcgttcctgctggtgtctcagatcattcgggggcttgcgatctgctacctctctttcagaaataagattattttcctgtccttctttacagttggcacgtggcgcagaatttatgcaaatgtcccttgagaagctatgtgtatacaactgtgtgctttcagctgacttcttctgcggtgactggccacatctgaataatattgggttatgtgggtgtagaagctctgcgtccttgtatgttggtgatcttacctccctcaaatcattctcactatatcatttgccagatttatgcgtgctggaaggtttgtcttacctacagcttcaccacgtgcatttgatagatgttccaaagcttactaccgagagcatctcacagttccgcgtacagcgttcgctctatattagcagttccgttatgctcaaccacatgatctccgctgaaggtttcaaagttccagggtttctctctcttgaaagctgcaagaagccatccgtttcattcgaagaatctgcaaatttcacatccgtcaagtgcctgaggttatgtaattgtgaaatgaggtcactaccaggaaatatgaagtgcctatccagtctaacgaaactcgatatctatgactgccccaacataacatctctaccagatctgccgtcctccctccagcatatatgtatatggggttgtgagcttttgaagaagagctgcagggcacctgatggagaaagctggccaaagattgcgcatatccgctggaaggaattcagatga</cdnaseq>|
AA = <aaseq>MAEVVLAGLRLAATPICVKLLCNASTCLGVDMTRELHELETIII PQFELVIEAAEKGNHRAKLDRWLRELKQAFYNAEDLLDEHEYNILKCKAKHKDSLVKD STQVHDSSISNILKQPMRAVSSRMSNLRPENRKILCQLNELKTMLEKAKEFRELIHLP AGNSLEGPSVPTIVVPVVTSLLPPRVFGRNMDRDRIIHLLTKPMATVSSSVGYSGLAI VAHGGAGKSTLAQCVYNDKRVQEHFDVRIWVCISRKLDVHRHTREIIESATNGECPRV DNLDTLQCRLKDIMQKSEKFLLVLDDVWFDESVNEREWDQLLDPLVSQQEGSRVLVTS RRDVLPAALHCKDVVHLENMEDAEFLALFKYHAFSGTEIRNPQLHARLEEVAEKIAKR LGQSPLAARTVGSQLSRNKDIAIWKSALNIENLSEPMKALLWSYNKLDSRLQRCFLYC SLFPKGHKYKIDEMVDLWVAEGLVDSRNQGDKRIEDIGRDYFNEMVSGSFFQPVSERY MGTWYIMHDLLHDLAESLTKEDCFRLEDDGVKEIPATVRHLSICVDSMKFHKQKICKL RYLRTVICIDPLMDDGDDIFNQLLKNLKKLRVLHLSFYNSSSLPECIGELKHLRYLSI ISTLISELPRSLCTLFHLELLHLNDKVKNLPDRLCNLRKLRRLEAYDDRNRMYKLYRA ALPQIPYIGKLSLLQDIDGFCVQKQKGYELRQLRDMNKLGGNLRVVNLENVTGKDEAS ESKLHQKTHLRGLHLSWNDVDDMDVSHLEILEGLRPPSQLEDLTIEGYKSTMYPSWLL DGSYFENLESFTLANCCVIGSLPPNTEIFRHCMTLTLENVPNMKTLPFLPEGLTSLSI EGCPLLVFTTNNDELEHHDYRESITRANNLETQLVLIWEANSDSDIRSTLSSEHSSMK KLTELMDTDMSGNLQTIESALEIERDEALVKEDIIKVWLCCHEERMRFIYSRKAGLPL VLPSGLCVLSLSSCSITDGALAICLGGLTSLRNLFLTEIMTLTTLPPEEVFQHLGNLR YLVIRSCWCLRSFGGLRSATSLSEIRLFSCPSLQLARGAEFMQMSLEKLCVYNCVLSA DFFCGDWPHLNNIGLCGCRSSASLYVGDLTSLKSFSLYHLPDLCVLEGLSYLQLHHVH LIDVPKLTTESISQFRVQRSLYISSSVMLNHMISAEGFKVPGFLSLESCKKPSVSFEE SANFTSVKCLRLCNCEMRSLPGNMKCLSSLTKLDIYDCPNITSLPDLPSSLQHICIWG CELLKKSCRAPDGESWPKIAHIRWKEFR</aaseq>|
DNA = <dnaseqindica>4282..8154#gggatctcccgccgttcggagacctaggggagcaagggggagactagagaaggttgcgctccatgaagtcatggtgcaccttaggtatcttgaaaggttttaccaagcgacctggttattgaagcgtggagaagaggctccagtgcggcaggagaaagcagatgagtagaggtacgatctcttcaggagaaagcagagcccagctgcccaggtggtggggagagattatcaaatttttcggtggtggtttggcacgggactctgccgcttcgctgccttcaaggctcaagctcagggtgactggcggcatctgacaggttcatttcctcttgcttatgtcttttagttgcaaatcaattaccattggttgtgggtttgaattattgggcgaaatgctcatgcgttggtcttgaaatttagctttttatctatctctatgttttacattataagtcgctttaatttttttctaattatttttttaagtttgatcaagttcatataaaaatgcaacaacattttcaactcaaaacaaatatattaatataatatattcaatgctatatgtaatgaaactaatttagtgttatgaatgttactatatttttttataaagttggttaaatctaaaaaggtttaattaaaaaaaatcaaaactacttataatacaagtaaaatagagagaattgcatagaagtggaagtgtgtcggtgtctgtactggttctactcacctagtcacctccctgccactgctattctgctaatagtgttagagaatacgcacgcacagataatggcaaggacgtcatttagaagagcccactagtgcgtgtcatgttgcttatgtgcaaattttatacactttcttgactgaccaaaggttgtatgcggtcattttttttttctctcggtgctagctttaatatacggaaagccgtttagagaagatggcatgacaataatggagcatatatatataaactagctcgtaccctgcggcgttgctgcgggtagatattgtaattgcaagtgtccagagaataacaaataaaattttccatggaacaatcatgatggccaaattgtataatatatggctgaaagttcacattatctaaaacaaatatttttcgctgcggtcggagtgtatattaagaaggagagagtttaagcaaactgtacaagagaacaatcagagccaaagctcacatacagaaccaggacactggttcgccacacacacatggttcaaacttcaaacattactgggagtctgatatacatttctttcttgtcttcctgcaaatttgtgttggcactagcttcatacagacctcaactctactgatccaagaacaacatgtagggctacatcctacatgtatacccaaatacccaatacaaacacaaaatagacgaagctaccctgcaatgcaatcagtaataagatcaatagttgataccgaagccaccagccagcatttcgggtaaacatagagcaacaatgaatataagcaagtctcttaagaattacgttctcattttgataaactggcaagcacgggtgaattttgcatcaacacccaacggaaatttgattagcattccttgaaatatgtcactgttgatgaaggtaaaaaagtaaacacaaagtgaagcccatagagcttcagtttagttcaggcatcaacatagtttaactcatgaacctccaaattaaataaattatttatttcattattatatatcataccttggtacatttgtcagcctcacggcagtagattttgcccatgttgcagtaagttacacacacaaacaccaaatatgctagtttttaaactattcttgtttcactagatttaattgcatttggttttttactatgctatttcggagttgtttaatttgatagtgggggccatagagtataatgaaaaacagcaaggtaatgagaaccagagcatagtccggctagaatattttaggagtccttttaatgaggtccagcttgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattctccaatttctccaaattgtaacatggtatcagacccttgttccaaaaaccctagccaccaaatccgatcatcaccggcgcaccggcggcggtgagccgccggcgagccatcatcatcgtcctccttcatccctcaacgtttttgtctccaagcagcaagcctgcagttcgtcgtcgtcaccgtgttcctgttcgccggtcgcagccgccgccagttgcggcgccgcgcccgttcccacttctccacccacgcagccacgccgtcgtggtgttcatccacgctgctcgtcgcggggctcgttcttgccgccgttcgagagccgccgctgtggcctcgtcaatctacatcaaggcatcaagcaacatcagtttttggcccatttcttgtcagttagcatcagcaaaccatcgtcctctctgatctgcagcagcaacaacaacaactcccgttcacctcctagtagatgcagcagctagcccattgttgtctcctaatttcagcagtagaatttccatgttccattgtttgtgttgcaacatgcattctgcagattgtcatcaagcttgtattccaccacatgtgttagtcagtttttgcgcttgctaatcgctgccatccactactcactcatcaatcatcaccatgctttgttattgttagctacttattataatccaattttacttctccaaattatcatgatggcttgtagtagtcactggttaggtcattagctgcttgcttgctactgtcaacatgttcactagcatcctttgtgtcttttgctagtaatgtgcatctctgcttgcagtccatctatcaatattgcctttttgctatatccatcaatccgtgttctttactaatcaaattcctgtgtttcctaccgagtccagcaaaatccattgcatatccttcattttgtcgaagctgtgccagtccatctcttcatggtcatttcatcatcaccagctccccgtcggagctggccttcattgtcacttcatatcttcatcatctccatgtcggagcttgtcatcatcatcatcaatcagtgaaggtccgaagaacagtttgtgaagggccttcatcaatatcaatgtctcatcatcaatcaaaggaccgaagaacagcttgtgaaggtccattgtcatcatcatcaacatctccgaatcaatgaagagccgaagaacgattcgtgaaggcttcaaggtaccgagggccgaagaacagtttgtgaaggctccgaggtccatttcattgaggagggggtcttttcttcttcatcatccaagaattgacacacttcgacgacatcatcaagactttggagataggctaatatttagattgtgttctcatgtttagtttacctttcaaatgcaatgttattacatacacattgcatttgagtgggggtgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattccccaatttctccaaattgtgacacagctcatctgaatattagagaaatataaatttgccactaacatttaacaatgtcccacttgtctactccctccgtcaaaaaaaaccaaacctaagacttagatgtgatataatctagtatctgtctagattcgttgtactagattatggcttaaatactagattaagttttttttgggacggagggagtatgtctaaccaaaataacacaattgatttgcatattgtgttttgcctcgcattgtgccgaagcaatcttatctgtggcaattggtagagttcttcctctcacaccttctctgcaatacaaacgcatctggctctggtaaccttgcactcctctcttagtatttctttccttcacgctttcttgtctctgcttaagttctctatcgtttggtaccatcatgcattaattcctttttcctctatttcccactccaaacatttccataaccactcacatacacaaaaataaaaaaccagacagagagaaaaaaacttgttttgggagttctgttgttatcttcctcacagaagaaaaatctctccatcaccatctgttcttatgccctgcttctattcattcacttgttcacagctgcagtagtgctgttccatggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaatgtgtttacaatgacaagagggtacaagaacattttgacgtcaggatatgggtctgcatctcacgcaaacttgatgttcatcgccatacacgtgagattatcgagtcagcaacaaatggagagtgccctcgtgtggacaatcttgatactctccagtgcagattgaaggacataatgcaaaaatcagagaaattcctgcttgtgttggatgacgtctggtttgatgaatccgtcaatgagagggaatgggaccagttgcttgatcctcttgtttctcagcaggaggggagcagagttttggtgacttctagacgagatgtacttccagctgctcttcactgtaaggatgttgttcatttggaaaacatggaagatgccgagttcttggctctattcaaataccatgctttctctggaacagaaatcagaaatccacagttgcatgcacggctagaagaggtcgcagagaagattgctaaaaggttaggacaatcgcctttagcagcaaggacagtaggctcccagctgagcaggaataaggatattgccatatggaaaagtgctctaaatattgaaaacttaagtgagcccatgaaagctctgttgtggagttataataaattagattcacgtctgcagagatgttttctgtactgcagcttatttccaaaaggtcacaagtataaaattgatgagatggttgacctttgggtggcagaggggctagttgattcacgcaaccagggggataagagaattgaagatattggcagggattacttcaatgagatggtgtcaggatctttctttcaaccagtttctgaaagatatatgggtacatggtacattatgcatgatctgcttcatgatttggcagagtcactgactaaagaagactgcttcagattagaagatgatggggtgaaagagataccagccactgttcgacatctatctatttgtgttgacagtatgaaattccataagcaaaagatctgcaagctacgttatttacgcactgttatctgcattgaccctctcatggatgacggagatgatatttttaatcagctattgaagaatctgaagaagctacgtgtactacatttgtcgttttacaacagtagcagcttgcctgaatgtattggtgagctgaagcaccttcgatatttgagtatcatcagcacattgatttctgaactgccaagatcattgtgtactcttttccacttggagctacttcatttgaatgacaaggtcaagaatttgccggacagactctgtaatttaagaaagttacgacgtcttgaagcatatgatgacagaaacagaatgtataaattgtatagagcagctctccctcaaattccttacataggcaagctatctttgctgcaagatattgatggattttgcgtgcaaaagcagaagggatatgagttgcgacaactgagggacatgaacaagctcggtggtaatttgcgtgtcgttaatcttgaaaatgtaactggaaaggatgaagcctcagagtcgaagttgcatcagaaaactcatcttagaggtttgcatctctcctggaatgatgtggatgacatggatgtttcacatttggagattctagaaggtttgaggccgccatctcaattggaggaccttacaatcgaaggttacaaatccaccatgtacccaagctggttacttgatggatcctattttgagaatctagagtcttttacgcttgctaattgctgtgtgataggaagcctaccacccaataccgagatctttaggcactgtatgacacttacccttgagaatgtcccgaatatgaagacactaccttttcttccagaaggtctcacaagcctatcaattgaggggtgcccactgcttgtgttcactactaataacgatgaactggaacaccatgattatagggagagcatcacgagggcaaacaacctggaaacacaacttgttttgatatgggaagcgaattccgattcagatattaggagcacattgtcgtccgaacactcatccatgaagaagttgacggaattgatggatactgatatgtcgggaaatcttcaaaccattgaaagcgctttagaaatagagagagatgaagccttggttaaagaagatatcatcaaagtatggctctgttgccacgaggagaggatgagattcatttattcaaggaaggctgggttgccgttggttctaccatctggactatgcgtactctctctttcctcctgtagtattacagatggagctttagctatttgccttggtgggcttacttcacttagaaatttgttcttaacagagattatgactttaactacgcttccaccggaagaggtcttccaacatttgggcaatcttagatacttggtcattcgttcctgctggtgtctcagatcattcgggggcttgcgatctgctacctctctttcagaaataagattattttcctgtccttctttacagttggcacgtggcgcagaatttatgcaaatgtcccttgagaagctatgtgtatacaactgtgtgctttcagctgacttcttctgcggtgactggccacatctgaataatattgggttatgtgggtgtagaagctctgcgtccttgtatgttggtgatcttacctccctcaaatcattctcactatatcatttgccagatttatgcgtgctggaaggtttgtcttacctacagcttcaccacgtgcatttgatagatgttccaaagcttactaccgagagcatctcacagttccgcgtacagcgttcgctctatattagcagttccgttatgctcaaccacatgatctccgctgaaggtttcaaagttccagggtttctctctcttgaaagctgcaagaagccatccgtttcattcgaagaatctgcaaatttcacatccgtcaagtgcctgaggttatgtaattgtgaaatgaggtcactaccaggaaatatgaagtgcctatccagtctaacgaaactcgatatctatgactgccccaacataacatctctaccagatctgccgtcctccctccagcatatatgtatatggggttgtgagcttttgaagaagagctgcagggcacctgatggagaaagctggccaaagattgcgcatatccgctggaaggaattcagatgaattgtgcttcagaatcactactacggagaagcgcaaaggtgaaggtcccatcaatgcattatcagaagtactctatgtaatatttatttcattcgctcatgatcttacgaacacatcattaacttcacgtttcctttgttttgcagacaccccaagatttgctctctcaggtaacttcgtctcttcgccacctgctacaaggtagagcgcctcatgctcatatagcagtttcagctctctcctgtagtcctgtggtctgtccggttgtgctgtccactctgttccctgcttcgtcgattgccacatttacgtttccctgctgccttctaccgacccacgcctcccttcatccggtctctgccactgacggcccatgatcgtgtccttttgctttcaccccttaaatggagtcttgttggtccaagaaaatctctgtttgtctgttgaacactcaactatctctcgtattttgtgtgatatcgctcgtatttgattgcgtgatgtgataatatattgaagtaattggattttgtacttcgcac</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185663.1 RefSeq:Os01g0782100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

Os01g0782100

2014-05-28T02:56:59Z

Liangdan2013: /* Expression */

Please input one-sentence summary here.
This rice blast resistance genes was found by two Japanese scholars Imbe and Matsumoto in 1985, they called this gene Pi-sh,this gene has the moderate resistance to the blast fungus strain Kyu77-07A in rice variety Shin-2.this gene has been characterized by using the method of classical genetic.And this gene is linking to pi-t.
==Annotated Information==
===Function===
The genes are accounting for the moderate resistance of the rice variety Shin 2 and some other varieties to the blast fungus strain Kyu 77-07A. Aichi Asahi (Pi-a) and carried out in the present study. Yashiro-mochi (Pi-ta), were found to be susceptible to the strain, while Ishikari Shirol*.e (Pi-i), Kanto 51 (Pi-k), Tsuyuake (Pi-k'!b), Fukunishiki (Pi-z) and Toride I (Pi-zt) were resistant, and Shin 2 (Pi-ks) and Pi N0.4 (Pi-ta2) moderately resistant. Therefore, Kyu 77-07A was classified as one of the strains belonging to race 102. Howver, the variety Reiho with the resistance gene Pi-ta2 was susceptible to this strain. Based on the knovn facts mentioned above, the objectives 0L the present study were' to determinA- whether Pi-ks is the gene responsible for the moderate resistance of Shin 2' to Kyu 77-07A along with the reason for the different reactions to Kyu 77-07A betweerL Pi N0.4 and Reiho which both have the same resistance gene Pi-ta2. For the gene analysis, the F2 or F3 plants of the crosses were inoculated with Kyu 77-07A and three other fungus strains using the spraying rnethod when the plants were at the four- to five-1eaf stage. Two varieties, Mineyutaka. and Saikai 155 belonging to the Shin 2 type varieties, were used as the representative parents of the varieties susceptible to Kyu 77-07A for the crosses.

===Expression===
To identify novel factors required for R gene-mediated resistance in rice, we used rice insertional mutant lines, induced by the endogenous retrotransposon Tos17, in a forward screen involving the rice blast fungus Magnaporthe oryzae. We inoculated 41,119 mutant lines with the fungus using a high throughput procedure, and identified 86 mutant lines with diminished resistance. A genome analysis revealed that 72 of the 86 lines contained mutations in a gene encoding a nucleotide binding site (NB) and leucine rich repeat (LRR) domain-containing (NLR) protein. A genetic complementation analysis and a pathogenesis assay demonstrated that this NLR gene encodes Pish, which confers resistance against races of M. oryzae containing avrPish. The other 14 lines have intact copies of the Pish gene, suggesting that they may contain mutations in the signaling components downstream of Pish. The genome analysis indicated that Pish and its neighboring three NLR genes are high similar to one another and are tandemly located. An in silico analysis of a Tos17 flanking sequence database revealed that this region is a "hot spot" for insertion. Intriguingly, the insertion sites are not distributed evenly among these four NLR genes, despite their similarity at the sequence and expression levels.
In this work we isolated the R gene Pish, and identified several other mutants involved in the signal transduction required for Pish-mediated resistance. These results indicate that our genetic approach is efficient and useful for unveiling novel aspects of defense signaling in rice. Furthermore, our data provide experimental evidence that R gene clusters have the potential to be highly preferred targets for transposable element insertions in plant genomes. Based on this finding, a possible mechanism underlying the high variability of R genes is discussed.
Primer pairs utilized in this work.

[[File:sequence.jpg]]

===Evolution===
A study was conducted to investigate the relationship between phenotype of blast resistance and resistance gene analog polymorphism of rice, and search molecule hereditary basis of broad spectrum and durable resistance.
【Method】 Comparison of clustering analysis was investigated using spectrum of resistance to blast and polymorphism of resistance gene analog (RGA) in 25 varieties for blast resistance identification and 20 varieties lines).
【Result】 The resistance spectrum clustering analysis showed that the 45 varieties (lines) could be divided into group A and group B with the genetic similarity coefficient of 0.450. Group A and group B could be divided into subclassⅰ, subclass , ⅱ subclass , ⅲ subclass ⅳ, respectively, with 0.618 genetic similarity coefficient. The RGA-PCR clustering analysis showed that proposed the 45 varieties (lines) could be divided into group Ⅰ and group Ⅱ which clearly inclined the Indica-japonica differentiation with 0.620 genetic similarity coefficient. Group Ⅰ could be divided into six subclasses and group Ⅱ could be divided into seven subclasses with 0.783 genetic similarity coefficient. The resistance spectrum clustering analysis showed that some varieties with similar resistance spectrum could finely fall into the same group, while the RGA-PCR clustering analysis showed that some varieties with the similar genetic background could fall into the same group. For some varieties with low resistance frequency or high resistance frequency, there was a better corresponding relationship between the resistance spectrum clustering and the RGA-PCR clustering. General comparison of clustering analysis showed that here was no parallelism relationship between group and group in two different types of the clustering.
【Conclusion】 It could more accurately reflect their enetic background to test resistance to single strain and analysis on RGA polymorphism for resistance parents, and avoid applying the same source of resistance gain and again, and enrich rice resistance germplasm, and breed durable resistance varieties.

You can also add sub-section(s) at will.

==Labs working on this gene==
1.Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Ibaraki, 305-8602，Japan.
2.International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines;
3.Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, Japan;
4.Indonesian Center for Rice Research (ICRR), JL. Raya Muara No. 25A Ciapus Bogor, Subang, West Java, Indonesia;
5.Agricultural Genetics Institute,Conhue, Tuliem, Hanoi, Vietnam;
6.Faculty of Agricultural Sciences, University of Burundi, BP 2940 Bujumbura, Burundi;

==References==
1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta
Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.)
Plant Breeding, 2010, 129(6): 670-675
2. Akira Takahashi;Nagao Hayashi;Akio Miyao;Hirohiko Hirochika
Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging
BMC Plant Biology, 2010, 10: 175
3. Tokio IMBE;Shohei MATSUMOTO
Inheritance of Resrstance of Rice Vanetles to the Blast Fungus Strains Virulent to the Variety "Reiho"
Japanese Journal of Breeding, 1985, 35(0): 332-339.
4.鄂志国, 张丽靖, 焦桂爱, 等. 稻瘟病抗性基因的鉴定及利用进展[J]. 中国水稻科学, 2008, 22(5): 533-540.
5.杨勤忠, 林菲, 冯淑杰, 等. 水稻稻瘟病抗性基因的分子定位及克隆研究进展[J]. 中国农业科学, 2009, 42(5): 1601-1615.
6.任鄄胜, 肖培村, 陈勇, 等. 水稻品种稻瘟病抗性和抗病基因同源序列多态性分析[J]. 中国农业科学, 2009, 42(1): 1-9.
==Structured Information==
{{JaponicaGene|
GeneName = Os01g0782100|
Description = Similar to NBS-LRR protein (Fragment)|
Version = NM_001185663.1 GI:297720460 GeneID:9271927|
Length = 8696 bp|
Definition = Oryza sativa Japonica Group Os01g0782100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:34892692..34901387|
CDS = 34896973..34900845|
GCID = <gbrowseImage1>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaatgtgtttacaatgacaagagggtacaagaacattttgacgtcaggatatgggtctgcatctcacgcaaacttgatgttcatcgccatacacgtgagattatcgagtcagcaacaaatggagagtgccctcgtgtggacaatcttgatactctccagtgcagattgaaggacataatgcaaaaatcagagaaattcctgcttgtgttggatgacgtctggtttgatgaatccgtcaatgagagggaatgggaccagttgcttgatcctcttgtttctcagcaggaggggagcagagttttggtgacttctagacgagatgtacttccagctgctcttcactgtaaggatgttgttcatttggaaaacatggaagatgccgagttcttggctctattcaaataccatgctttctctggaacagaaatcagaaatccacagttgcatgcacggctagaagaggtcgcagagaagattgctaaaaggttaggacaatcgcctttagcagcaaggacagtaggctcccagctgagcaggaataaggatattgccatatggaaaagtgctctaaatattgaaaacttaagtgagcccatgaaagctctgttgtggagttataataaattagattcacgtctgcagagatgttttctgtactgcagcttatttccaaaaggtcacaagtataaaattgatgagatggttgacctttgggtggcagaggggctagttgattcacgcaaccagggggataagagaattgaagatattggcagggattacttcaatgagatggtgtcaggatctttctttcaaccagtttctgaaagatatatgggtacatggtacattatgcatgatctgcttcatgatttggcagagtcactgactaaagaagactgcttcagattagaagatgatggggtgaaagagataccagccactgttcgacatctatctatttgtgttgacagtatgaaattccataagcaaaagatctgcaagctacgttatttacgcactgttatctgcattgaccctctcatggatgacggagatgatatttttaatcagctattgaagaatctgaagaagctacgtgtactacatttgtcgttttacaacagtagcagcttgcctgaatgtattggtgagctgaagcaccttcgatatttgagtatcatcagcacattgatttctgaactgccaagatcattgtgtactcttttccacttggagctacttcatttgaatgacaaggtcaagaatttgccggacagactctgtaatttaagaaagttacgacgtcttgaagcatatgatgacagaaacagaatgtataaattgtatagagcagctctccctcaaattccttacataggcaagctatctttgctgcaagatattgatggattttgcgtgcaaaagcagaagggatatgagttgcgacaactgagggacatgaacaagctcggtggtaatttgcgtgtcgttaatcttgaaaatgtaactggaaaggatgaagcctcagagtcgaagttgcatcagaaaactcatcttagaggtttgcatctctcctggaatgatgtggatgacatggatgtttcacatttggagattctagaaggtttgaggccgccatctcaattggaggaccttacaatcgaaggttacaaatccaccatgtacccaagctggttacttgatggatcctattttgagaatctagagtcttttacgcttgctaattgctgtgtgataggaagcctaccacccaataccgagatctttaggcactgtatgacacttacccttgagaatgtcccgaatatgaagacactaccttttcttccagaaggtctcacaagcctatcaattgaggggtgcccactgcttgtgttcactactaataacgatgaactggaacaccatgattatagggagagcatcacgagggcaaacaacctggaaacacaacttgttttgatatgggaagcgaattccgattcagatattaggagcacattgtcgtccgaacactcatccatgaagaagttgacggaattgatggatactgatatgtcgggaaatcttcaaaccattgaaagcgctttagaaatagagagagatgaagccttggttaaagaagatatcatcaaagtatggctctgttgccacgaggagaggatgagattcatttattcaaggaaggctgggttgccgttggttctaccatctggactatgcgtactctctctttcctcctgtagtattacagatggagctttagctatttgccttggtgggcttacttcacttagaaatttgttcttaacagagattatgactttaactacgcttccaccggaagaggtcttccaacatttgggcaatcttagatacttggtcattcgttcctgctggtgtctcagatcattcgggggcttgcgatctgctacctctctttcagaaataagattattttcctgtccttctttacagttggcacgtggcgcagaatttatgcaaatgtcccttgagaagctatgtgtatacaactgtgtgctttcagctgacttcttctgcggtgactggccacatctgaataatattgggttatgtgggtgtagaagctctgcgtccttgtatgttggtgatcttacctccctcaaatcattctcactatatcatttgccagatttatgcgtgctggaaggtttgtcttacctacagcttcaccacgtgcatttgatagatgttccaaagcttactaccgagagcatctcacagttccgcgtacagcgttcgctctatattagcagttccgttatgctcaaccacatgatctccgctgaaggtttcaaagttccagggtttctctctcttgaaagctgcaagaagccatccgtttcattcgaagaatctgcaaatttcacatccgtcaagtgcctgaggttatgtaattgtgaaatgaggtcactaccaggaaatatgaagtgcctatccagtctaacgaaactcgatatctatgactgccccaacataacatctctaccagatctgccgtcctccctccagcatatatgtatatggggttgtgagcttttgaagaagagctgcagggcacctgatggagaaagctggccaaagattgcgcatatccgctggaaggaattcagatga</cdnaseq>|
AA = <aaseq>MAEVVLAGLRLAATPICVKLLCNASTCLGVDMTRELHELETIII PQFELVIEAAEKGNHRAKLDRWLRELKQAFYNAEDLLDEHEYNILKCKAKHKDSLVKD STQVHDSSISNILKQPMRAVSSRMSNLRPENRKILCQLNELKTMLEKAKEFRELIHLP AGNSLEGPSVPTIVVPVVTSLLPPRVFGRNMDRDRIIHLLTKPMATVSSSVGYSGLAI VAHGGAGKSTLAQCVYNDKRVQEHFDVRIWVCISRKLDVHRHTREIIESATNGECPRV DNLDTLQCRLKDIMQKSEKFLLVLDDVWFDESVNEREWDQLLDPLVSQQEGSRVLVTS RRDVLPAALHCKDVVHLENMEDAEFLALFKYHAFSGTEIRNPQLHARLEEVAEKIAKR LGQSPLAARTVGSQLSRNKDIAIWKSALNIENLSEPMKALLWSYNKLDSRLQRCFLYC SLFPKGHKYKIDEMVDLWVAEGLVDSRNQGDKRIEDIGRDYFNEMVSGSFFQPVSERY MGTWYIMHDLLHDLAESLTKEDCFRLEDDGVKEIPATVRHLSICVDSMKFHKQKICKL RYLRTVICIDPLMDDGDDIFNQLLKNLKKLRVLHLSFYNSSSLPECIGELKHLRYLSI ISTLISELPRSLCTLFHLELLHLNDKVKNLPDRLCNLRKLRRLEAYDDRNRMYKLYRA ALPQIPYIGKLSLLQDIDGFCVQKQKGYELRQLRDMNKLGGNLRVVNLENVTGKDEAS ESKLHQKTHLRGLHLSWNDVDDMDVSHLEILEGLRPPSQLEDLTIEGYKSTMYPSWLL DGSYFENLESFTLANCCVIGSLPPNTEIFRHCMTLTLENVPNMKTLPFLPEGLTSLSI EGCPLLVFTTNNDELEHHDYRESITRANNLETQLVLIWEANSDSDIRSTLSSEHSSMK KLTELMDTDMSGNLQTIESALEIERDEALVKEDIIKVWLCCHEERMRFIYSRKAGLPL VLPSGLCVLSLSSCSITDGALAICLGGLTSLRNLFLTEIMTLTTLPPEEVFQHLGNLR YLVIRSCWCLRSFGGLRSATSLSEIRLFSCPSLQLARGAEFMQMSLEKLCVYNCVLSA DFFCGDWPHLNNIGLCGCRSSASLYVGDLTSLKSFSLYHLPDLCVLEGLSYLQLHHVH LIDVPKLTTESISQFRVQRSLYISSSVMLNHMISAEGFKVPGFLSLESCKKPSVSFEE SANFTSVKCLRLCNCEMRSLPGNMKCLSSLTKLDIYDCPNITSLPDLPSSLQHICIWG CELLKKSCRAPDGESWPKIAHIRWKEFR</aaseq>|
DNA = <dnaseqindica>4282..8154#gggatctcccgccgttcggagacctaggggagcaagggggagactagagaaggttgcgctccatgaagtcatggtgcaccttaggtatcttgaaaggttttaccaagcgacctggttattgaagcgtggagaagaggctccagtgcggcaggagaaagcagatgagtagaggtacgatctcttcaggagaaagcagagcccagctgcccaggtggtggggagagattatcaaatttttcggtggtggtttggcacgggactctgccgcttcgctgccttcaaggctcaagctcagggtgactggcggcatctgacaggttcatttcctcttgcttatgtcttttagttgcaaatcaattaccattggttgtgggtttgaattattgggcgaaatgctcatgcgttggtcttgaaatttagctttttatctatctctatgttttacattataagtcgctttaatttttttctaattatttttttaagtttgatcaagttcatataaaaatgcaacaacattttcaactcaaaacaaatatattaatataatatattcaatgctatatgtaatgaaactaatttagtgttatgaatgttactatatttttttataaagttggttaaatctaaaaaggtttaattaaaaaaaatcaaaactacttataatacaagtaaaatagagagaattgcatagaagtggaagtgtgtcggtgtctgtactggttctactcacctagtcacctccctgccactgctattctgctaatagtgttagagaatacgcacgcacagataatggcaaggacgtcatttagaagagcccactagtgcgtgtcatgttgcttatgtgcaaattttatacactttcttgactgaccaaaggttgtatgcggtcattttttttttctctcggtgctagctttaatatacggaaagccgtttagagaagatggcatgacaataatggagcatatatatataaactagctcgtaccctgcggcgttgctgcgggtagatattgtaattgcaagtgtccagagaataacaaataaaattttccatggaacaatcatgatggccaaattgtataatatatggctgaaagttcacattatctaaaacaaatatttttcgctgcggtcggagtgtatattaagaaggagagagtttaagcaaactgtacaagagaacaatcagagccaaagctcacatacagaaccaggacactggttcgccacacacacatggttcaaacttcaaacattactgggagtctgatatacatttctttcttgtcttcctgcaaatttgtgttggcactagcttcatacagacctcaactctactgatccaagaacaacatgtagggctacatcctacatgtatacccaaatacccaatacaaacacaaaatagacgaagctaccctgcaatgcaatcagtaataagatcaatagttgataccgaagccaccagccagcatttcgggtaaacatagagcaacaatgaatataagcaagtctcttaagaattacgttctcattttgataaactggcaagcacgggtgaattttgcatcaacacccaacggaaatttgattagcattccttgaaatatgtcactgttgatgaaggtaaaaaagtaaacacaaagtgaagcccatagagcttcagtttagttcaggcatcaacatagtttaactcatgaacctccaaattaaataaattatttatttcattattatatatcataccttggtacatttgtcagcctcacggcagtagattttgcccatgttgcagtaagttacacacacaaacaccaaatatgctagtttttaaactattcttgtttcactagatttaattgcatttggttttttactatgctatttcggagttgtttaatttgatagtgggggccatagagtataatgaaaaacagcaaggtaatgagaaccagagcatagtccggctagaatattttaggagtccttttaatgaggtccagcttgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattctccaatttctccaaattgtaacatggtatcagacccttgttccaaaaaccctagccaccaaatccgatcatcaccggcgcaccggcggcggtgagccgccggcgagccatcatcatcgtcctccttcatccctcaacgtttttgtctccaagcagcaagcctgcagttcgtcgtcgtcaccgtgttcctgttcgccggtcgcagccgccgccagttgcggcgccgcgcccgttcccacttctccacccacgcagccacgccgtcgtggtgttcatccacgctgctcgtcgcggggctcgttcttgccgccgttcgagagccgccgctgtggcctcgtcaatctacatcaaggcatcaagcaacatcagtttttggcccatttcttgtcagttagcatcagcaaaccatcgtcctctctgatctgcagcagcaacaacaacaactcccgttcacctcctagtagatgcagcagctagcccattgttgtctcctaatttcagcagtagaatttccatgttccattgtttgtgttgcaacatgcattctgcagattgtcatcaagcttgtattccaccacatgtgttagtcagtttttgcgcttgctaatcgctgccatccactactcactcatcaatcatcaccatgctttgttattgttagctacttattataatccaattttacttctccaaattatcatgatggcttgtagtagtcactggttaggtcattagctgcttgcttgctactgtcaacatgttcactagcatcctttgtgtcttttgctagtaatgtgcatctctgcttgcagtccatctatcaatattgcctttttgctatatccatcaatccgtgttctttactaatcaaattcctgtgtttcctaccgagtccagcaaaatccattgcatatccttcattttgtcgaagctgtgccagtccatctcttcatggtcatttcatcatcaccagctccccgtcggagctggccttcattgtcacttcatatcttcatcatctccatgtcggagcttgtcatcatcatcatcaatcagtgaaggtccgaagaacagtttgtgaagggccttcatcaatatcaatgtctcatcatcaatcaaaggaccgaagaacagcttgtgaaggtccattgtcatcatcatcaacatctccgaatcaatgaagagccgaagaacgattcgtgaaggcttcaaggtaccgagggccgaagaacagtttgtgaaggctccgaggtccatttcattgaggagggggtcttttcttcttcatcatccaagaattgacacacttcgacgacatcatcaagactttggagataggctaatatttagattgtgttctcatgtttagtttacctttcaaatgcaatgttattacatacacattgcatttgagtgggggtgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattccccaatttctccaaattgtgacacagctcatctgaatattagagaaatataaatttgccactaacatttaacaatgtcccacttgtctactccctccgtcaaaaaaaaccaaacctaagacttagatgtgatataatctagtatctgtctagattcgttgtactagattatggcttaaatactagattaagttttttttgggacggagggagtatgtctaaccaaaataacacaattgatttgcatattgtgttttgcctcgcattgtgccgaagcaatcttatctgtggcaattggtagagttcttcctctcacaccttctctgcaatacaaacgcatctggctctggtaaccttgcactcctctcttagtatttctttccttcacgctttcttgtctctgcttaagttctctatcgtttggtaccatcatgcattaattcctttttcctctatttcccactccaaacatttccataaccactcacatacacaaaaataaaaaaccagacagagagaaaaaaacttgttttgggagttctgttgttatcttcctcacagaagaaaaatctctccatcaccatctgttcttatgccctgcttctattcattcacttgttcacagctgcagtagtgctgttccatggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaat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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185663.1 RefSeq:Os01g0782100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

Os01g0782100

2014-05-28T02:56:08Z

Liangdan2013: /* Expression */

Please input one-sentence summary here.
This rice blast resistance genes was found by two Japanese scholars Imbe and Matsumoto in 1985, they called this gene Pi-sh,this gene has the moderate resistance to the blast fungus strain Kyu77-07A in rice variety Shin-2.this gene has been characterized by using the method of classical genetic.And this gene is linking to pi-t.
==Annotated Information==
===Function===
The genes are accounting for the moderate resistance of the rice variety Shin 2 and some other varieties to the blast fungus strain Kyu 77-07A. Aichi Asahi (Pi-a) and carried out in the present study. Yashiro-mochi (Pi-ta), were found to be susceptible to the strain, while Ishikari Shirol*.e (Pi-i), Kanto 51 (Pi-k), Tsuyuake (Pi-k'!b), Fukunishiki (Pi-z) and Toride I (Pi-zt) were resistant, and Shin 2 (Pi-ks) and Pi N0.4 (Pi-ta2) moderately resistant. Therefore, Kyu 77-07A was classified as one of the strains belonging to race 102. Howver, the variety Reiho with the resistance gene Pi-ta2 was susceptible to this strain. Based on the knovn facts mentioned above, the objectives 0L the present study were' to determinA- whether Pi-ks is the gene responsible for the moderate resistance of Shin 2' to Kyu 77-07A along with the reason for the different reactions to Kyu 77-07A betweerL Pi N0.4 and Reiho which both have the same resistance gene Pi-ta2. For the gene analysis, the F2 or F3 plants of the crosses were inoculated with Kyu 77-07A and three other fungus strains using the spraying rnethod when the plants were at the four- to five-1eaf stage. Two varieties, Mineyutaka. and Saikai 155 belonging to the Shin 2 type varieties, were used as the representative parents of the varieties susceptible to Kyu 77-07A for the crosses.

===Expression===
To identify novel factors required for R gene-mediated resistance in rice, we used rice insertional mutant lines, induced by the endogenous retrotransposon Tos17, in a forward screen involving the rice blast fungus Magnaporthe oryzae. We inoculated 41,119 mutant lines with the fungus using a high throughput procedure, and identified 86 mutant lines with diminished resistance. A genome analysis revealed that 72 of the 86 lines contained mutations in a gene encoding a nucleotide binding site (NB) and leucine rich repeat (LRR) domain-containing (NLR) protein. A genetic complementation analysis and a pathogenesis assay demonstrated that this NLR gene encodes Pish, which confers resistance against races of M. oryzae containing avrPish. The other 14 lines have intact copies of the Pish gene, suggesting that they may contain mutations in the signaling components downstream of Pish. The genome analysis indicated that Pish and its neighboring three NLR genes are high similar to one another and are tandemly located. An in silico analysis of a Tos17 flanking sequence database revealed that this region is a "hot spot" for insertion. Intriguingly, the insertion sites are not distributed evenly among these four NLR genes, despite their similarity at the sequence and expression levels.
In this work we isolated the R gene Pish, and identified several other mutants involved in the signal transduction required for Pish-mediated resistance. These results indicate that our genetic approach is efficient and useful for unveiling novel aspects of defense signaling in rice. Furthermore, our data provide experimental evidence that R gene clusters have the potential to be highly preferred targets for transposable element insertions in plant genomes. Based on this finding, a possible mechanism underlying the high variability of R genes is discussed.
Primer pairs utilized in this work.
[[File:sequence.jpg]]

===Evolution===
A study was conducted to investigate the relationship between phenotype of blast resistance and resistance gene analog polymorphism of rice, and search molecule hereditary basis of broad spectrum and durable resistance.
【Method】 Comparison of clustering analysis was investigated using spectrum of resistance to blast and polymorphism of resistance gene analog (RGA) in 25 varieties for blast resistance identification and 20 varieties lines).
【Result】 The resistance spectrum clustering analysis showed that the 45 varieties (lines) could be divided into group A and group B with the genetic similarity coefficient of 0.450. Group A and group B could be divided into subclassⅰ, subclass , ⅱ subclass , ⅲ subclass ⅳ, respectively, with 0.618 genetic similarity coefficient. The RGA-PCR clustering analysis showed that proposed the 45 varieties (lines) could be divided into group Ⅰ and group Ⅱ which clearly inclined the Indica-japonica differentiation with 0.620 genetic similarity coefficient. Group Ⅰ could be divided into six subclasses and group Ⅱ could be divided into seven subclasses with 0.783 genetic similarity coefficient. The resistance spectrum clustering analysis showed that some varieties with similar resistance spectrum could finely fall into the same group, while the RGA-PCR clustering analysis showed that some varieties with the similar genetic background could fall into the same group. For some varieties with low resistance frequency or high resistance frequency, there was a better corresponding relationship between the resistance spectrum clustering and the RGA-PCR clustering. General comparison of clustering analysis showed that here was no parallelism relationship between group and group in two different types of the clustering.
【Conclusion】 It could more accurately reflect their enetic background to test resistance to single strain and analysis on RGA polymorphism for resistance parents, and avoid applying the same source of resistance gain and again, and enrich rice resistance germplasm, and breed durable resistance varieties.

You can also add sub-section(s) at will.

==Labs working on this gene==
1.Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Ibaraki, 305-8602，Japan.
2.International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines;
3.Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, Japan;
4.Indonesian Center for Rice Research (ICRR), JL. Raya Muara No. 25A Ciapus Bogor, Subang, West Java, Indonesia;
5.Agricultural Genetics Institute,Conhue, Tuliem, Hanoi, Vietnam;
6.Faculty of Agricultural Sciences, University of Burundi, BP 2940 Bujumbura, Burundi;

==References==
1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta
Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.)
Plant Breeding, 2010, 129(6): 670-675
2. Akira Takahashi;Nagao Hayashi;Akio Miyao;Hirohiko Hirochika
Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging
BMC Plant Biology, 2010, 10: 175
3. Tokio IMBE;Shohei MATSUMOTO
Inheritance of Resrstance of Rice Vanetles to the Blast Fungus Strains Virulent to the Variety "Reiho"
Japanese Journal of Breeding, 1985, 35(0): 332-339.
4.鄂志国, 张丽靖, 焦桂爱, 等. 稻瘟病抗性基因的鉴定及利用进展[J]. 中国水稻科学, 2008, 22(5): 533-540.
5.杨勤忠, 林菲, 冯淑杰, 等. 水稻稻瘟病抗性基因的分子定位及克隆研究进展[J]. 中国农业科学, 2009, 42(5): 1601-1615.
6.任鄄胜, 肖培村, 陈勇, 等. 水稻品种稻瘟病抗性和抗病基因同源序列多态性分析[J]. 中国农业科学, 2009, 42(1): 1-9.
==Structured Information==
{{JaponicaGene|
GeneName = Os01g0782100|
Description = Similar to NBS-LRR protein (Fragment)|
Version = NM_001185663.1 GI:297720460 GeneID:9271927|
Length = 8696 bp|
Definition = Oryza sativa Japonica Group Os01g0782100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:34892692..34901387|
CDS = 34896973..34900845|
GCID = <gbrowseImage1>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaatgtgtttacaatgacaagagggtacaagaacattttgacgtcaggatatgggtctgcatctcacgcaaacttgatgttcatcgccatacacgtgagattatcgagtcagcaacaaatggagagtgccctcgtgtggacaatcttgatactctccagtgcagattgaaggacataatgcaaaaatcagagaaattcctgcttgtgttggatgacgtctggtttgatgaatccgtcaatgagagggaatgggaccagttgcttgatcctcttgtttctcagcaggaggggagcagagttttggtgacttctagacgagatgtacttccagctgctcttcactgtaaggatgttgttcatttggaaaacatggaagatgccgagttcttggctctattcaaataccatgctttctctggaacagaaatcagaaatccacagttgcatgcacggctagaagaggtcgcagagaagattgctaaaaggttaggacaatcgcctttagcagcaaggacagtaggctcccagctgagcaggaataaggatattgccatatggaaaagtgctctaaatattgaaaacttaagtgagcccatgaaagctctgttgtggagttataataaattagattcacgtctgcagagatgttttctgtactgcagcttatttccaaaaggtcacaagtataaaattgatgagatggttgacctttgggtggcagaggggctagttgattcacgcaaccagggggataagagaattgaagatattggcagggattacttcaatgagatggtgtcaggatctttctttcaaccagtttctgaaagatatatgggtacatggtacattatgcatgatctgcttcatgatttggcagagtcactgactaaagaagactgcttcagattagaagatgatggggtgaaagagataccagccactgttcgacatctatctatttgtgttgacagtatgaaattccataagcaaaagatctgcaagctacgttatttacgcactgttatctgcattgaccctctcatggatgacggagatgatatttttaatcagctattgaagaatctgaagaagctacgtgtactacatttgtcgttttacaacagtagcagcttgcctgaatgtattggtgagctgaagcaccttcgatatttgagtatcatcagcacattgatttctgaactgccaagatcattgtgtactcttttccacttggagctacttcatttgaatgacaaggtcaagaatttgccggacagactctgtaatttaagaaagttacgacgtcttgaagcatatgatgacagaaacagaatgtataaattgtatagagcagctctccctcaaattccttacataggcaagctatctttgctgcaagatattgatggattttgcgtgcaaaagcagaagggatatgagttgcgacaactgagggacatgaacaagctcggtggtaatttgcgtgtcgttaatcttgaaaatgtaactggaaaggatgaagcctcagagtcgaagttgcatcagaaaactcatcttagaggtttgcatctctcctggaatgatgtggatgacatggatgtttcacatttggagattctagaaggtttgaggccgccatctcaattggaggaccttacaatcgaaggttacaaatccaccatgtacccaagctggttacttgatggatcctattttgagaatctagagtcttttacgcttgctaattgctgtgtgataggaagcctaccacccaataccgagatctttaggcactgtatgacacttacccttgagaatgtcccgaatatgaagacactaccttttcttccagaaggtctcacaagcctatcaattgaggggtgcccactgcttgtgttcactactaataacgatgaactggaacaccatgattatagggagagcatcacgagggcaaacaacctggaaacacaacttgttttgatatgggaagcgaattccgattcagatattaggagcacattgtcgtccgaacactcatccatgaagaagttgacggaattgatggatactgatatgtcgggaaatcttcaaaccattgaaagcgctttagaaatagagagagatgaagccttggttaaagaagatatcatcaaagtatggctctgttgccacgaggagaggatgagattcatttattcaaggaaggctgggttgccgttggttctaccatctggactatgcgtactctctctttcctcctgtagtattacagatggagctttagctatttgccttggtgggcttacttcacttagaaatttgttcttaacagagattatgactttaactacgcttccaccggaagaggtcttccaacatttgggcaatcttagatacttggtcattcgttcctgctggtgtctcagatcattcgggggcttgcgatctgctacctctctttcagaaataagattattttcctgtccttctttacagttggcacgtggcgcagaatttatgcaaatgtcccttgagaagctatgtgtatacaactgtgtgctttcagctgacttcttctgcggtgactggccacatctgaataatattgggttatgtgggtgtagaagctctgcgtccttgtatgttggtgatcttacctccctcaaatcattctcactatatcatttgccagatttatgcgtgctggaaggtttgtcttacctacagcttcaccacgtgcatttgatagatgttccaaagcttactaccgagagcatctcacagttccgcgtacagcgttcgctctatattagcagttccgttatgctcaaccacatgatctccgctgaaggtttcaaagttccagggtttctctctcttgaaagctgcaagaagccatccgtttcattcgaagaatctgcaaatttcacatccgtcaagtgcctgaggttatgtaattgtgaaatgaggtcactaccaggaaatatgaagtgcctatccagtctaacgaaactcgatatctatgactgccccaacataacatctctaccagatctgccgtcctccctccagcatatatgtatatggggttgtgagcttttgaagaagagctgcagggcacctgatggagaaagctggccaaagattgcgcatatccgctggaaggaattcagatga</cdnaseq>|
AA = <aaseq>MAEVVLAGLRLAATPICVKLLCNASTCLGVDMTRELHELETIII PQFELVIEAAEKGNHRAKLDRWLRELKQAFYNAEDLLDEHEYNILKCKAKHKDSLVKD STQVHDSSISNILKQPMRAVSSRMSNLRPENRKILCQLNELKTMLEKAKEFRELIHLP AGNSLEGPSVPTIVVPVVTSLLPPRVFGRNMDRDRIIHLLTKPMATVSSSVGYSGLAI VAHGGAGKSTLAQCVYNDKRVQEHFDVRIWVCISRKLDVHRHTREIIESATNGECPRV DNLDTLQCRLKDIMQKSEKFLLVLDDVWFDESVNEREWDQLLDPLVSQQEGSRVLVTS RRDVLPAALHCKDVVHLENMEDAEFLALFKYHAFSGTEIRNPQLHARLEEVAEKIAKR LGQSPLAARTVGSQLSRNKDIAIWKSALNIENLSEPMKALLWSYNKLDSRLQRCFLYC SLFPKGHKYKIDEMVDLWVAEGLVDSRNQGDKRIEDIGRDYFNEMVSGSFFQPVSERY MGTWYIMHDLLHDLAESLTKEDCFRLEDDGVKEIPATVRHLSICVDSMKFHKQKICKL RYLRTVICIDPLMDDGDDIFNQLLKNLKKLRVLHLSFYNSSSLPECIGELKHLRYLSI ISTLISELPRSLCTLFHLELLHLNDKVKNLPDRLCNLRKLRRLEAYDDRNRMYKLYRA ALPQIPYIGKLSLLQDIDGFCVQKQKGYELRQLRDMNKLGGNLRVVNLENVTGKDEAS ESKLHQKTHLRGLHLSWNDVDDMDVSHLEILEGLRPPSQLEDLTIEGYKSTMYPSWLL DGSYFENLESFTLANCCVIGSLPPNTEIFRHCMTLTLENVPNMKTLPFLPEGLTSLSI EGCPLLVFTTNNDELEHHDYRESITRANNLETQLVLIWEANSDSDIRSTLSSEHSSMK KLTELMDTDMSGNLQTIESALEIERDEALVKEDIIKVWLCCHEERMRFIYSRKAGLPL VLPSGLCVLSLSSCSITDGALAICLGGLTSLRNLFLTEIMTLTTLPPEEVFQHLGNLR YLVIRSCWCLRSFGGLRSATSLSEIRLFSCPSLQLARGAEFMQMSLEKLCVYNCVLSA DFFCGDWPHLNNIGLCGCRSSASLYVGDLTSLKSFSLYHLPDLCVLEGLSYLQLHHVH LIDVPKLTTESISQFRVQRSLYISSSVMLNHMISAEGFKVPGFLSLESCKKPSVSFEE SANFTSVKCLRLCNCEMRSLPGNMKCLSSLTKLDIYDCPNITSLPDLPSSLQHICIWG CELLKKSCRAPDGESWPKIAHIRWKEFR</aaseq>|
DNA = <dnaseqindica>4282..8154#gggatctcccgccgttcggagacctaggggagcaagggggagactagagaaggttgcgctccatgaagtcatggtgcaccttaggtatcttgaaaggttttaccaagcgacctggttattgaagcgtggagaagaggctccagtgcggcaggagaaagcagatgagtagaggtacgatctcttcaggagaaagcagagcccagctgcccaggtggtggggagagattatcaaatttttcggtggtggtttggcacgggactctgccgcttcgctgccttcaaggctcaagctcagggtgactggcggcatctgacaggttcatttcctcttgcttatgtcttttagttgcaaatcaattaccattggttgtgggtttgaattattgggcgaaatgctcatgcgttggtcttgaaatttagctttttatctatctctatgttttacattataagtcgctttaatttttttctaattatttttttaagtttgatcaagttcatataaaaatgcaacaacattttcaactcaaaacaaatatattaatataatatattcaatgctatatgtaatgaaactaatttagtgttatgaatgttactatatttttttataaagttggttaaatctaaaaaggtttaattaaaaaaaatcaaaactacttataatacaagtaaaatagagagaattgcatagaagtggaagtgtgtcggtgtctgtactggttctactcacctagtcacctccctgccactgctattctgctaatagtgttagagaatacgcacgcacagataatggcaaggacgtcatttagaagagcccactagtgcgtgtcatgttgcttatgtgcaaattttatacactttcttgactgaccaaaggttgtatgcggtcattttttttttctctcggtgctagctttaatatacggaaagccgtttagagaagatggcatgacaataatggagcatatatatataaactagctcgtaccctgcggcgttgctgcgggtagatattgtaattgcaagtgtccagagaataacaaataaaattttccatggaacaatcatgatggccaaattgtataatatatggctgaaagttcacattatctaaaacaaatatttttcgctgcggtcggagtgtatattaagaaggagagagtttaagcaaactgtacaagagaacaatcagagccaaagctcacatacagaaccaggacactggttcgccacacacacatggttcaaacttcaaacattactgggagtctgatatacatttctttcttgtcttcctgcaaatttgtgttggcactagcttcatacagacctcaactctactgatccaagaacaacatgtagggctacatcctacatgtatacccaaatacccaatacaaacacaaaatagacgaagctaccctgcaatgcaatcagtaataagatcaatagttgataccgaagccaccagccagcatttcgggtaaacatagagcaacaatgaatataagcaagtctcttaagaattacgttctcattttgataaactggcaagcacgggtgaattttgcatcaacacccaacggaaatttgattagcattccttgaaatatgtcactgttgatgaaggtaaaaaagtaaacacaaagtgaagcccatagagcttcagtttagttcaggcatcaacatagtttaactcatgaacctccaaattaaataaattatttatttcattattatatatcataccttggtacatttgtcagcctcacggcagtagattttgcccatgttgcagtaagttacacacacaaacaccaaatatgctagtttttaaactattcttgtttcactagatttaattgcatttggttttttactatgctatttcggagttgtttaatttgatagtgggggccatagagtataatgaaaaacagcaaggtaatgagaaccagagcatagtccggctagaatattttaggagtccttttaatgaggtccagcttgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattctccaatttctccaaattgtaacatggtatcagacccttgttccaaaaaccctagccaccaaatccgatcatcaccggcgcaccggcggcggtgagccgccggcgagccatcatcatcgtcctccttcatccctcaacgtttttgtctccaagcagcaagcctgcagttcgtcgtcgtcaccgtgttcctgttcgccggtcgcagccgccgccagttgcggcgccgcgcccgttcccacttctccacccacgcagccacgccgtcgtggtgttcatccacgctgctcgtcgcggggctcgttcttgccgccgttcgagagccgccgctgtggcctcgtcaatctacatcaaggcatcaagcaacatcagtttttggcccatttcttgtcagttagcatcagcaaaccatcgtcctctctgatctgcagcagcaacaacaacaactcccgttcacctcctagtagatgcagcagctagcccattgttgtctcctaatttcagcagtagaatttccatgttccattgtttgtgttgcaacatgcattctgcagattgtcatcaagcttgtattccaccacatgtgttagtcagtttttgcgcttgctaatcgctgccatccactactcactcatcaatcatcaccatgctttgttattgttagctacttattataatccaattttacttctccaaattatcatgatggcttgtagtagtcactggttaggtcattagctgcttgcttgctactgtcaacatgttcactagcatcctttgtgtcttttgctagtaatgtgcatctctgcttgcagtccatctatcaatattgcctttttgctatatccatcaatccgtgttctttactaatcaaattcctgtgtttcctaccgagtccagcaaaatccattgcatatccttcattttgtcgaagctgtgccagtccatctcttcatggtcatttcatcatcaccagctccccgtcggagctggccttcattgtcacttcatatcttcatcatctccatgtcggagcttgtcatcatcatcatcaatcagtgaaggtccgaagaacagtttgtgaagggccttcatcaatatcaatgtctcatcatcaatcaaaggaccgaagaacagcttgtgaaggtccattgtcatcatcatcaacatctccgaatcaatgaagagccgaagaacgattcgtgaaggcttcaaggtaccgagggccgaagaacagtttgtgaaggctccgaggtccatttcattgaggagggggtcttttcttcttcatcatccaagaattgacacacttcgacgacatcatcaagactttggagataggctaatatttagattgtgttctcatgtttagtttacctttcaaatgcaatgttattacatacacattgcatttgagtgggggtgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattccccaatttctccaaattgtgacacagctcatctgaatattagagaaatataaatttgccactaacatttaacaatgtcccacttgtctactccctccgtcaaaaaaaaccaaacctaagacttagatgtgatataatctagtatctgtctagattcgttgtactagattatggcttaaatactagattaagttttttttgggacggagggagtatgtctaaccaaaataacacaattgatttgcatattgtgttttgcctcgcattgtgccgaagcaatcttatctgtggcaattggtagagttcttcctctcacaccttctctgcaatacaaacgcatctggctctggtaaccttgcactcctctcttagtatttctttccttcacgctttcttgtctctgcttaagttctctatcgtttggtaccatcatgcattaattcctttttcctctatttcccactccaaacatttccataaccactcacatacacaaaaataaaaaaccagacagagagaaaaaaacttgttttgggagttctgttgttatcttcctcacagaagaaaaatctctccatcaccatctgttcttatgccctgcttctattcattcacttgttcacagctgcagtagtgctgttccatggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaatgtgtttacaatgacaagagggtacaagaacattttgacgtcaggatatgggtctgcatctcacgcaaacttgatgttcatcgccatacacgtgagattatcgagtcagcaacaaatggagagtgccctcgtgtggacaatcttgatactctccagtgcagattgaaggacataatgcaaaaatcagagaaattcctgcttgtgttggatgacgtctggtttgatgaatccgtcaatgagagggaatgggaccagttgcttgatcctcttgtttctcagcaggaggggagcagagttttggtgacttctagacgagatgtacttccagctgctcttcactgtaaggatgttgttcatttggaaaacatggaagatgccgagttcttggctctattcaaataccatgctttctctggaacagaaatcagaaatccacagttgcatgcacggctagaagaggtcgcagagaagattgctaaaaggttaggacaatcgcctttagcagcaaggacagtaggctcccagctgagcaggaataaggatattgccatatggaaaagtgctctaaatattgaaaacttaagtgagcccatgaaagctctgttgtggagttataataaattagattcacgtctgcagagatgttttctgtactgcagcttatttccaaaaggtcacaagtataaaattgatgagatggttgacctttgggtggcagaggggctagttgattcacgcaaccagggggataagagaattgaagatattggcagggattacttcaatgagatggtgtcaggatctttctttcaaccagtttctgaaagatatatgggtacatggtacattatgcatgatctgcttcatgatttggcagagtcactgactaaagaagactgcttcagattagaagatgatggggtgaaagagataccagccactgttcgacatctatctatttgtgttgacagtatgaaattccataagcaaaagatctgcaagctacgttatttacgcactgttatctgcattgaccctctcatggatgacggagatgatatttttaatcagctattgaagaatctgaagaagctacgtgtactacatttgtcgttttacaacagtagcagcttgcctgaatgtattggtgagctgaagcaccttcgatatttgagtatcatcagcacattgatttctgaactgccaagatcattgtgtactcttttccacttggagctacttcatttgaatgacaaggtcaagaatttgccggacagactctgtaatttaagaaagttacgacgtcttgaagcatatgatgacagaaacagaatgtataaattgtatagagcagctctccctcaaattccttacataggcaagctatctttgctgcaagatattgatggattttgcgtgcaaaagcagaagggatatgagttgcgacaactgagggacatgaacaagctcggtggtaatttgcgtgtcgttaatcttgaaaatgtaactggaaaggatgaagcctcagagtcgaagttgcatcagaaaactcatcttagaggtttgcatctctcctggaatgatgtggatgacatggatgtttcacatttggagattctagaaggtttgaggccgccatctcaattggaggaccttacaatcgaaggttacaaatccaccatgtacccaagctggttacttgatggatcctattttgagaatctagagtcttttacgcttgctaattgctgtgtgataggaagcctaccacccaataccgagatctttaggcactgtatgacacttacccttgagaatgtcccgaatatgaagacactaccttttcttccagaaggtctcacaagcctatcaattgaggggtgcccactgcttgtgttcactactaataacgatgaactggaacaccatgattatagggagagcatcacgagggcaaacaacctggaaacacaacttgttttgatatgggaagcgaattccgattcagatattaggagcacattgtcgtccgaacactcatccatgaagaagttgacggaattgatggatactgatatgtcgggaaatcttcaaaccattgaaagcgctttagaaatagagagagatgaagccttggttaaagaagatatcatcaaagtatggctctgttgccacgaggagaggatgagattcatttattcaaggaaggctgggttgccgttggttctaccatctggactatgcgtactctctctttcctcctgtagtattacagatggagctttagctatttgccttggtgggcttacttcacttagaaatttgttcttaacagagattatgactttaactacgcttccaccggaagaggtcttccaacatttgggcaatcttagatacttggtcattcgttcctgctggtgtctcagatcattcgggggcttgcgatctgctacctctctttcagaaataagattattttcctgtccttctttacagttggcacgtggcgcagaatttatgcaaatgtcccttgagaagctatgtgtatacaactgtgtgctttcagctgacttcttctgcggtgactggccacatctgaataatattgggttatgtgggtgtagaagctctgcgtccttgtatgttggtgatcttacctccctcaaatcattctcactatatcatttgccagatttatgcgtgctggaaggtttgtcttacctacagcttcaccacgtgcatttgatagatgttccaaagcttactaccgagagcatctcacagttccgcgtacagcgttcgctctatattagcagttccgttatgctcaaccacatgatctccgctgaaggtttcaaagttccagggtttctctctcttgaaagctgcaagaagccatccgtttcattcgaagaatctgcaaatttcacatccgtcaagtgcctgaggttatgtaattgtgaaatgaggtcactaccaggaaatatgaagtgcctatccagtctaacgaaactcgatatctatgactgccccaacataacatctctaccagatctgccgtcctccctccagcatatatgtatatggggttgtgagcttttgaagaagagctgcagggcacctgatggagaaagctggccaaagattgcgcatatccgctggaaggaattcagatgaattgtgcttcagaatcactactacggagaagcgcaaaggtgaaggtcccatcaatgcattatcagaagtactctatgtaatatttatttcattcgctcatgatcttacgaacacatcattaacttcacgtttcctttgttttgcagacaccccaagatttgctctctcaggtaacttcgtctcttcgccacctgctacaaggtagagcgcctcatgctcatatagcagtttcagctctctcctgtagtcctgtggtctgtccggttgtgctgtccactctgttccctgcttcgtcgattgccacatttacgtttccctgctgccttctaccgacccacgcctcccttcatccggtctctgccactgacggcccatgatcgtgtccttttgctttcaccccttaaatggagtcttgttggtccaagaaaatctctgtttgtctgttgaacactcaactatctctcgtattttgtgtgatatcgctcgtatttgattgcgtgatgtgataatatattgaagtaattggattttgtacttcgcac</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185663.1 RefSeq:Os01g0782100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

Os01g0782100

2014-05-28T02:55:18Z

Liangdan2013: /* Expression */

Please input one-sentence summary here.
This rice blast resistance genes was found by two Japanese scholars Imbe and Matsumoto in 1985, they called this gene Pi-sh,this gene has the moderate resistance to the blast fungus strain Kyu77-07A in rice variety Shin-2.this gene has been characterized by using the method of classical genetic.And this gene is linking to pi-t.
==Annotated Information==
===Function===
The genes are accounting for the moderate resistance of the rice variety Shin 2 and some other varieties to the blast fungus strain Kyu 77-07A. Aichi Asahi (Pi-a) and carried out in the present study. Yashiro-mochi (Pi-ta), were found to be susceptible to the strain, while Ishikari Shirol*.e (Pi-i), Kanto 51 (Pi-k), Tsuyuake (Pi-k'!b), Fukunishiki (Pi-z) and Toride I (Pi-zt) were resistant, and Shin 2 (Pi-ks) and Pi N0.4 (Pi-ta2) moderately resistant. Therefore, Kyu 77-07A was classified as one of the strains belonging to race 102. Howver, the variety Reiho with the resistance gene Pi-ta2 was susceptible to this strain. Based on the knovn facts mentioned above, the objectives 0L the present study were' to determinA- whether Pi-ks is the gene responsible for the moderate resistance of Shin 2' to Kyu 77-07A along with the reason for the different reactions to Kyu 77-07A betweerL Pi N0.4 and Reiho which both have the same resistance gene Pi-ta2. For the gene analysis, the F2 or F3 plants of the crosses were inoculated with Kyu 77-07A and three other fungus strains using the spraying rnethod when the plants were at the four- to five-1eaf stage. Two varieties, Mineyutaka. and Saikai 155 belonging to the Shin 2 type varieties, were used as the representative parents of the varieties susceptible to Kyu 77-07A for the crosses.

===Expression===
To identify novel factors required for R gene-mediated resistance in rice, we used rice insertional mutant lines, induced by the endogenous retrotransposon Tos17, in a forward screen involving the rice blast fungus Magnaporthe oryzae. We inoculated 41,119 mutant lines with the fungus using a high throughput procedure, and identified 86 mutant lines with diminished resistance. A genome analysis revealed that 72 of the 86 lines contained mutations in a gene encoding a nucleotide binding site (NB) and leucine rich repeat (LRR) domain-containing (NLR) protein. A genetic complementation analysis and a pathogenesis assay demonstrated that this NLR gene encodes Pish, which confers resistance against races of M. oryzae containing avrPish. The other 14 lines have intact copies of the Pish gene, suggesting that they may contain mutations in the signaling components downstream of Pish. The genome analysis indicated that Pish and its neighboring three NLR genes are high similar to one another and are tandemly located. An in silico analysis of a Tos17 flanking sequence database revealed that this region is a "hot spot" for insertion. Intriguingly, the insertion sites are not distributed evenly among these four NLR genes, despite their similarity at the sequence and expression levels.
In this work we isolated the R gene Pish, and identified several other mutants involved in the signal transduction required for Pish-mediated resistance. These results indicate that our genetic approach is efficient and useful for unveiling novel aspects of defense signaling in rice. Furthermore, our data provide experimental evidence that R gene clusters have the potential to be highly preferred targets for transposable element insertions in plant genomes. Based on this finding, a possible mechanism underlying the high variability of R genes is discussed.
[[File:sequence.jpg]]

===Evolution===
A study was conducted to investigate the relationship between phenotype of blast resistance and resistance gene analog polymorphism of rice, and search molecule hereditary basis of broad spectrum and durable resistance.
【Method】 Comparison of clustering analysis was investigated using spectrum of resistance to blast and polymorphism of resistance gene analog (RGA) in 25 varieties for blast resistance identification and 20 varieties lines).
【Result】 The resistance spectrum clustering analysis showed that the 45 varieties (lines) could be divided into group A and group B with the genetic similarity coefficient of 0.450. Group A and group B could be divided into subclassⅰ, subclass , ⅱ subclass , ⅲ subclass ⅳ, respectively, with 0.618 genetic similarity coefficient. The RGA-PCR clustering analysis showed that proposed the 45 varieties (lines) could be divided into group Ⅰ and group Ⅱ which clearly inclined the Indica-japonica differentiation with 0.620 genetic similarity coefficient. Group Ⅰ could be divided into six subclasses and group Ⅱ could be divided into seven subclasses with 0.783 genetic similarity coefficient. The resistance spectrum clustering analysis showed that some varieties with similar resistance spectrum could finely fall into the same group, while the RGA-PCR clustering analysis showed that some varieties with the similar genetic background could fall into the same group. For some varieties with low resistance frequency or high resistance frequency, there was a better corresponding relationship between the resistance spectrum clustering and the RGA-PCR clustering. General comparison of clustering analysis showed that here was no parallelism relationship between group and group in two different types of the clustering.
【Conclusion】 It could more accurately reflect their enetic background to test resistance to single strain and analysis on RGA polymorphism for resistance parents, and avoid applying the same source of resistance gain and again, and enrich rice resistance germplasm, and breed durable resistance varieties.

You can also add sub-section(s) at will.

==Labs working on this gene==
1.Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Ibaraki, 305-8602，Japan.
2.International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines;
3.Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, Japan;
4.Indonesian Center for Rice Research (ICRR), JL. Raya Muara No. 25A Ciapus Bogor, Subang, West Java, Indonesia;
5.Agricultural Genetics Institute,Conhue, Tuliem, Hanoi, Vietnam;
6.Faculty of Agricultural Sciences, University of Burundi, BP 2940 Bujumbura, Burundi;

==References==
1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta
Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.)
Plant Breeding, 2010, 129(6): 670-675
2. Akira Takahashi;Nagao Hayashi;Akio Miyao;Hirohiko Hirochika
Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging
BMC Plant Biology, 2010, 10: 175
3. Tokio IMBE;Shohei MATSUMOTO
Inheritance of Resrstance of Rice Vanetles to the Blast Fungus Strains Virulent to the Variety "Reiho"
Japanese Journal of Breeding, 1985, 35(0): 332-339.
4.鄂志国, 张丽靖, 焦桂爱, 等. 稻瘟病抗性基因的鉴定及利用进展[J]. 中国水稻科学, 2008, 22(5): 533-540.
5.杨勤忠, 林菲, 冯淑杰, 等. 水稻稻瘟病抗性基因的分子定位及克隆研究进展[J]. 中国农业科学, 2009, 42(5): 1601-1615.
6.任鄄胜, 肖培村, 陈勇, 等. 水稻品种稻瘟病抗性和抗病基因同源序列多态性分析[J]. 中国农业科学, 2009, 42(1): 1-9.
==Structured Information==
{{JaponicaGene|
GeneName = Os01g0782100|
Description = Similar to NBS-LRR protein (Fragment)|
Version = NM_001185663.1 GI:297720460 GeneID:9271927|
Length = 8696 bp|
Definition = Oryza sativa Japonica Group Os01g0782100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:34892692..34901387|
CDS = 34896973..34900845|
GCID = <gbrowseImage1>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaatgtgtttacaatgacaagagggtacaagaacattttgacgtcaggatatgggtctgcatctcacgcaaacttgatgttcatcgccatacacgtgagattatcgagtcagcaacaaatggagagtgccctcgtgtggacaatcttgatactctccagtgcagattgaaggacataatgcaaaaatcagagaaattcctgcttgtgttggatgacgtctggtttgatgaatccgtcaatgagagggaatgggaccagttgcttgatcctcttgtttctcagcaggaggggagcagagttttggtgacttctagacgagatgtacttccagctgctcttcactgtaaggatgttgttcatttggaaaacatggaagatgccgagttcttggctctattcaaataccatgctttctctggaacagaaatcagaaatccacagttgcatgcacggctagaagaggtcgcagagaagattgctaaaaggttaggacaatcgcctttagcagcaaggacagtaggctcccagctgagcaggaataaggatattgccatatggaaaagtgctctaaatattgaaaacttaagtgagcccatgaaagctctgttgtggagttataataaattagattcacgtctgcagagatgttttctgtactgcagcttatttccaaaaggtcacaagtataaaattgatgagatggttgacctttgggtggcagaggggctagttgattcacgcaaccagggggataagagaattgaagatattggcagggattacttcaatgagatggtgtcaggatctttctttcaaccagtttctgaaagatatatgggtacatggtacattatgcatgatctgcttcatgatttggcagagtcactgactaaagaagactgcttcagattagaagatgatggggtgaaagagataccagccactgttcgacatctatctatttgtgttgacagtatgaaattccataagcaaaagatctgcaagctacgttatttacgcactgttatctgcattgaccctctcatggatgacggagatgatatttttaatcagctattgaagaatctgaagaagctacgtgtactacatttgtcgttttacaacagtagcagcttgcctgaatgtattggtgagctgaagcaccttcgatatttgagtatcatcagcacattgatttctgaactgccaagatcattgtgtactcttttccacttggagctacttcatttgaatgacaaggtcaagaatttgccggacagactctgtaatttaagaaagttacgacgtcttgaagcatatgatgacagaaacagaatgtataaattgtatagagcagctctccctcaaattccttacataggcaagctatctttgctgcaagatattgatggattttgcgtgcaaaagcagaagggatatgagttgcgacaactgagggacatgaacaagctcggtggtaatttgcgtgtcgttaatcttgaaaatgtaactggaaaggatgaagcctcagagtcgaagttgcatcagaaaactcatcttagaggtttgcatctctcctggaatgatgtggatgacatggatgtttcacatttggagattctagaaggtttgaggccgccatctcaattggaggaccttacaatcgaaggttacaaatccaccatgtacccaagctggttacttgatggatcctattttgagaatctagagtcttttacgcttgctaattgctgtgtgataggaagcctaccacccaataccgagatctttaggcactgtatgacacttacccttgagaatgtcccgaatatgaagacactaccttttcttccagaaggtctcacaagcctatcaattgaggggtgcccactgcttgtgttcactactaataacgatgaactggaacaccatgattatagggagagcatcacgagggcaaacaacctggaaacacaacttgttttgatatgggaagcgaattccgattcagatattaggagcacattgtcgtccgaacactcatccatgaagaagttgacggaattgatggatactgatatgtcgggaaatcttcaaaccattgaaagcgctttagaaatagagagagatgaagccttggttaaagaagatatcatcaaagtatggctctgttgccacgaggagaggatgagattcatttattcaaggaaggctgggttgccgttggttctaccatctggactatgcgtactctctctttcctcctgtagtattacagatggagctttagctatttgccttggtgggcttacttcacttagaaatttgttcttaacagagattatgactttaactacgcttccaccggaagaggtcttccaacatttgggcaatcttagatacttggtcattcgttcctgctggtgtctcagatcattcgggggcttgcgatctgctacctctctttcagaaataagattattttcctgtccttctttacagttggcacgtggcgcagaatttatgcaaatgtcccttgagaagctatgtgtatacaactgtgtgctttcagctgacttcttctgcggtgactggccacatctgaataatattgggttatgtgggtgtagaagctctgcgtccttgtatgttggtgatcttacctccctcaaatcattctcactatatcatttgccagatttatgcgtgctggaaggtttgtcttacctacagcttcaccacgtgcatttgatagatgttccaaagcttactaccgagagcatctcacagttccgcgtacagcgttcgctctatattagcagttccgttatgctcaaccacatgatctccgctgaaggtttcaaagttccagggtttctctctcttgaaagctgcaagaagccatccgtttcattcgaagaatctgcaaatttcacatccgtcaagtgcctgaggttatgtaattgtgaaatgaggtcactaccaggaaatatgaagtgcctatccagtctaacgaaactcgatatctatgactgccccaacataacatctctaccagatctgccgtcctccctccagcatatatgtatatggggttgtgagcttttgaagaagagctgcagggcacctgatggagaaagctggccaaagattgcgcatatccgctggaaggaattcagatga</cdnaseq>|
AA = <aaseq>MAEVVLAGLRLAATPICVKLLCNASTCLGVDMTRELHELETIII PQFELVIEAAEKGNHRAKLDRWLRELKQAFYNAEDLLDEHEYNILKCKAKHKDSLVKD STQVHDSSISNILKQPMRAVSSRMSNLRPENRKILCQLNELKTMLEKAKEFRELIHLP AGNSLEGPSVPTIVVPVVTSLLPPRVFGRNMDRDRIIHLLTKPMATVSSSVGYSGLAI VAHGGAGKSTLAQCVYNDKRVQEHFDVRIWVCISRKLDVHRHTREIIESATNGECPRV DNLDTLQCRLKDIMQKSEKFLLVLDDVWFDESVNEREWDQLLDPLVSQQEGSRVLVTS RRDVLPAALHCKDVVHLENMEDAEFLALFKYHAFSGTEIRNPQLHARLEEVAEKIAKR LGQSPLAARTVGSQLSRNKDIAIWKSALNIENLSEPMKALLWSYNKLDSRLQRCFLYC SLFPKGHKYKIDEMVDLWVAEGLVDSRNQGDKRIEDIGRDYFNEMVSGSFFQPVSERY MGTWYIMHDLLHDLAESLTKEDCFRLEDDGVKEIPATVRHLSICVDSMKFHKQKICKL RYLRTVICIDPLMDDGDDIFNQLLKNLKKLRVLHLSFYNSSSLPECIGELKHLRYLSI ISTLISELPRSLCTLFHLELLHLNDKVKNLPDRLCNLRKLRRLEAYDDRNRMYKLYRA ALPQIPYIGKLSLLQDIDGFCVQKQKGYELRQLRDMNKLGGNLRVVNLENVTGKDEAS ESKLHQKTHLRGLHLSWNDVDDMDVSHLEILEGLRPPSQLEDLTIEGYKSTMYPSWLL DGSYFENLESFTLANCCVIGSLPPNTEIFRHCMTLTLENVPNMKTLPFLPEGLTSLSI EGCPLLVFTTNNDELEHHDYRESITRANNLETQLVLIWEANSDSDIRSTLSSEHSSMK KLTELMDTDMSGNLQTIESALEIERDEALVKEDIIKVWLCCHEERMRFIYSRKAGLPL VLPSGLCVLSLSSCSITDGALAICLGGLTSLRNLFLTEIMTLTTLPPEEVFQHLGNLR YLVIRSCWCLRSFGGLRSATSLSEIRLFSCPSLQLARGAEFMQMSLEKLCVYNCVLSA DFFCGDWPHLNNIGLCGCRSSASLYVGDLTSLKSFSLYHLPDLCVLEGLSYLQLHHVH LIDVPKLTTESISQFRVQRSLYISSSVMLNHMISAEGFKVPGFLSLESCKKPSVSFEE SANFTSVKCLRLCNCEMRSLPGNMKCLSSLTKLDIYDCPNITSLPDLPSSLQHICIWG CELLKKSCRAPDGESWPKIAHIRWKEFR</aaseq>|
DNA = <dnaseqindica>4282..8154#gggatctcccgccgttcggagacctaggggagcaagggggagactagagaaggttgcgctccatgaagtcatggtgcaccttaggtatcttgaaaggttttaccaagcgacctggttattgaagcgtggagaagaggctccagtgcggcaggagaaagcagatgagtagaggtacgatctcttcaggagaaagcagagcccagctgcccaggtggtggggagagattatcaaatttttcggtggtggtttggcacgggactctgccgcttcgctgccttcaaggctcaagctcagggtgactggcggcatctgacaggttcatttcctcttgcttatgtcttttagttgcaaatcaattaccattggttgtgggtttgaattattgggcgaaatgctcatgcgttggtcttgaaatttagctttttatctatctctatgttttacattataagtcgctttaatttttttctaattatttttttaagtttgatcaagttcatataaaaatgcaacaacattttcaactcaaaacaaatatattaatataatatattcaatgctatatgtaatgaaactaatttagtgttatgaatgttactatatttttttataaagttggttaaatctaaaaaggtttaattaaaaaaaatcaaaactacttataatacaagtaaaatagagagaattgcatagaagtggaagtgtgtcggtgtctgtactggttctactcacctagtcacctccctgccactgctattctgctaatagtgttagagaatacgcacgcacagataatggcaaggacgtcatttagaagagcccactagtgcgtgtcatgttgcttatgtgcaaattttatacactttcttgactgaccaaaggttgtatgcggtcattttttttttctctcggtgctagctttaatatacggaaagccgtttagagaagatggcatgacaataatggagcatatatatataaactagctcgtaccctgcggcgttgctgcgggtagatattgtaattgcaagtgtccagagaataacaaataaaattttccatggaacaatcatgatggccaaattgtataatatatggctgaaagttcacattatctaaaacaaatatttttcgctgcggtcggagtgtatattaagaaggagagagtttaagcaaactgtacaagagaacaatcagagccaaagctcacatacagaaccaggacactggttcgccacacacacatggttcaaacttcaaacattactgggagtctgatatacatttctttcttgtcttcctgcaaatttgtgttggcactagcttcatacagacctcaactctactgatccaagaacaacatgtagggctacatcctacatgtatacccaaatacccaatacaaacacaaaatagacgaagctaccctgcaatgcaatcagtaataagatcaatagttgataccgaagccaccagccagcatttcgggtaaacatagagcaacaatgaatataagcaagtctcttaagaattacgttctcattttgataaactggcaagcacgggtgaattttgcatcaacacccaacggaaatttgattagcattccttgaaatatgtcactgttgatgaaggtaaaaaagtaaacacaaagtgaagcccatagagcttcagtttagttcaggcatcaacatagtttaactcatgaacctccaaattaaataaattatttatttcattattatatatcataccttggtacatttgtcagcctcacggcagtagattttgcccatgttgcagtaagttacacacacaaacaccaaatatgctagtttttaaactattcttgtttcactagatttaattgcatttggttttttactatgctatttcggagttgtttaatttgatagtgggggccatagagtataatgaaaaacagcaaggtaatgagaaccagagcatagtccggctagaatattttaggagtccttttaatgaggtccagcttgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattctccaatttctccaaattgtaacatggtatcagacccttgttccaaaaaccctagccaccaaatccgatcatcaccggcgcaccggcggcggtgagccgccggcgagccatcatcatcgtcctccttcatccctcaacgtttttgtctccaagcagcaagcctgcagttcgtcgtcgtcaccgtgttcctgttcgccggtcgcagccgccgccagttgcggcgccgcgcccgttcccacttctccacccacgcagccacgccgtcgtggtgttcatccacgctgctcgtcgcggggctcgttcttgccgccgttcgagagccgccgctgtggcctcgtcaatctacatcaaggcatcaagcaacatcagtttttggcccatttcttgtcagttagcatcagcaaaccatcgtcctctctgatctgcagcagcaacaacaacaactcccgttcacctcctagtagatgcagcagctagcccattgttgtctcctaatttcagcagtagaatttccatgttccattgtttgtgttgcaacatgcattctgcagattgtcatcaagcttgtattccaccacatgtgttagtcagtttttgcgcttgctaatcgctgccatccactactcactcatcaatcatcaccatgctttgttattgttagctacttattataatccaattttacttctccaaattatcatgatggcttgtagtagtcactggttaggtcattagctgcttgcttgctactgtcaacatgttcactagcatcctttgtgtcttttgctagtaatgtgcatctctgcttgcagtccatctatcaatattgcctttttgctatatccatcaatccgtgttctttactaatcaaattcctgtgtttcctaccgagtccagcaaaatccattgcatatccttcattttgtcgaagctgtgccagtccatctcttcatggtcatttcatcatcaccagctccccgtcggagctggccttcattgtcacttcatatcttcatcatctccatgtcggagcttgtcatcatcatcatcaatcagtgaaggtccgaagaacagtttgtgaagggccttcatcaatatcaatgtctcatcatcaatcaaaggaccgaagaacagcttgtgaaggtccattgtcatcatcatcaacatctccgaatcaatgaagagccgaagaacgattcgtgaaggcttcaaggtaccgagggccgaagaacagtttgtgaaggctccgaggtccatttcattgaggagggggtcttttcttcttcatcatccaagaattgacacacttcgacgacatcatcaagactttggagataggctaatatttagattgtgttctcatgtttagtttacctttcaaatgcaatgttattacatacacattgcatttgagtgggggtgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattccccaatttctccaaattgtgacacagctcatctgaatattagagaaatataaatttgccactaacatttaacaatgtcccacttgtctactccctccgtcaaaaaaaaccaaacctaagacttagatgtgatataatctagtatctgtctagattcgttgtactagattatggcttaaatactagattaagttttttttgggacggagggagtatgtctaaccaaaataacacaattgatttgcatattgtgttttgcctcgcattgtgccgaagcaatcttatctgtggcaattggtagagttcttcctctcacaccttctctgcaatacaaacgcatctggctctggtaaccttgcactcctctcttagtatttctttccttcacgctttcttgtctctgcttaagttctctatcgtttggtaccatcatgcattaattcctttttcctctatttcccactccaaacatttccataaccactcacatacacaaaaataaaaaaccagacagagagaaaaaaacttgttttgggagttctgttgttatcttcctcacagaagaaaaatctctccatcaccatctgttcttatgccctgcttctattcattcacttgttcacagctgcagtagtgctgttccatggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaat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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185663.1 RefSeq:Os01g0782100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

File:Sequence.jpg

2014-05-28T02:54:12Z

Liangdan2013:

Os03g0227700

2014-05-25T14:13:24Z

Liangdan2013:

Please input one-sentence summary here.
The gene OsDWARF4 was found in young elongating tissues.
==Annotated Information==

Brassinosteroids influence both plant height and leaf erectness in rice[1]. Arabidopsis thaliana BRASSINOSTEROID INSENSITIVE1 (bri1) mutants, which are deficient in the brassinosteroid receptor, have a severe dwarf phenotype[2]. The rice mutants d61-1 and d61-2, which are weak mutant alleles of OsBRI1, a homolog of A. thaliana BRI1, have both a semi-dwarf phenotype and more erect leaves[3]. In rice, an erect leaf phenotype has also been associated with loss-of-function, brassinosteroid-deficient mutants such as brassinosteroid-deficient dwarf1 (brd1), which is defective in OsDWARF (the homolog of tomato CYP85A1/DWARF[4]), and ebisu dwarf (d2), which is defective in CYP90D2/D2 (ref. [5]). However, the grain yield of d61, brd1 and d2 mutants is decreased because of morphological alterations in their reproductive development.

===Function===

New cultivars with very erect leaves, which increase light capture for photosynthesis and nitrogen storage for grain filling, may have increased grain yields.The erect leaf phenotype of a rice brassinosteroid-deficient mutant, osdwarf4-1, is associated with enhanced grain yields under conditions of dense planting, even without extra fertilizer. Molecular and biochemical studies reveal that two different cytochrome P450s, CYP90B2/OsDWARF4 and CYP724B1/D11, function redundantly in C-22 hydroxylation, the rate-limiting step of brassinosteroid biosynthesis. Therefore, despite the central role of brassinosteroids in plant growth and development, mutation of OsDWARF4 alone causes only limited defects in brassinosteroid biosynthesis and plant morphology[6].

===Expression===
The affected gene, OsDWARF4, was identified by characterizing the site of insertion of the Tos17 retrotransposon.

----Molecular characterization of OsDWARF4 and OsDWARF4L1/D11

OsDWARF4 is a homolog of A. thaliana DWARF4, which encodes a cytochrome P450, CYP90B1 (Fig. 1a). CYP90B1/DWARF4 catalyzes C-22 hydroxylation, the rate-limiting step of brassinosteroid biosynthesis[7]. Only one DWARF4 homolog was identified in the rice genome. OsDWARF4 is located on the short arm of chromosome 3 (35 cM). Its predicted open reading frame consists of eight exons, and encodes a protein of 502 amino acids (Fig. 1b,c). OsDWARF4 is most closely related to DWARF4 (66.3% amino-acid identity; Fig. 1a). The structure of OsDWARF4 is similar to that of DWARF4 throughout its length: five domains found in cytochrome P450s—proline rich, A (also referred to as dioxygen binding), B (steroid binding), C and heme binding—are highly conserved (Fig. 1c). In wild-type rice, OsDWARF4 transcripts are most abundant in leaf blades and roots, but are also found in all other plant parts tested (Fig. 1d). Transcript levels were decreased by exogenous brassinolide treatment, and increased in brassinosteroid-insensitive d61-3 and brassinosteroid-deficient brd1-1 (Fig. 1e). These results suggest that OsDWARF4 expression is feedback regulated, as is that of A. thaliana brassinosteroid-biosynthesis genes[8].
OsDWARF4L1 was identified as an OsDWARF4-like gene by in silico screening of rice DNA databases. OsDWARF4L1 is located on the long arm of chromosome 4 (70.9 cM). A recent study demonstrated that a rice semi-dwarf and short-grain mutant, d11, has a loss-of-function mutation in the OsDWARF4L1 gene[9]. OsDWARF4L1/D11 shows the highest homology with A. thaliana CYP724A1, and is closely related to rice OsDWARF4 and A. thaliana DWARF4 (47.4%, 37.7% and 41.7% amino-acid identities, respectively; Fig. 1a). In wild-type rice, OsDWARF4L1/D11 transcripts were most abundant in vegetative shoot apices, leaf sheaths and roots, and found at more moderate levels in other organs (Fig. 1d). They were decreased by exogenous brassinolide treatment, and increased in d61-3 and brd1-1 (Fig. 1e). These results indicate that OsDWARF4L1/D11 expression is regulated by a feedback mechanism, as is that of OsDWARF4.

----Phenotypes of rice mutants defective in brassinosteroid biosynthesis

In osdwarf4-1, Tos17 insertion into exon 6 causes a premature termination in domain C (arrowhead in Fig. 1b,c). Because a premature stop codon before the heme-binding domain caused loss-of-function of A. thaliana DWARF4 (ref. [7]), we consider osdwarf4-1 to be a null allele. Unexpectedly, osdwarf4-1 plants show only weak morphological phenotypes, such as a slightly dwarfed stature and more erect leaves (Fig. 2a,b). Both of these phenotypes were complemented by the introduction of an entire OsDWARF4 gene (data not shown). These results seem inconsistent with the fact that rice d61-3 and brd1-1, which are loss-of-function mutants of OsBRI1 and OsDWARF respectively, display severe dwarfing and have malformed leaves (for example, see brd1-1 in Fig. 2a), and suggest functional redundancy in brassinosteroid biosynthesis in rice.
Because null alleles of d11, such as d11-4, also show a weak phenotype[9] (Fig. 2a),OsDWARF4L1/D11 and OsDWARF4 may function redundantly. To confirm this possibility, researcher produced double mutants by crossing homozygous osdwarf4-1 and d11-4 plants. Among the F2 population, 34 of 576 plants showed severe dwarfing and malformed leaves with tortuous leaf blades, indistinguishable from the brassinosteroid-deficient brd1-1 phenotype (Fig. 2a,c,d). This ratio fits the theoretical 9:3:3:1 (wild-type:osdwarf4-1:d11-4:osdwarf4-1/d11-4) segregation predicted by mendelian inheritance. PCR and sequence analyses confirmed that these plants were osdwarf4-1/d11-4 double mutants (data not shown).
Endogenous levels of brassinosteroid intermediates in brd1 clearly indicate that OsDWARF catalyzes C-6 oxidation[4]. However, quantification in d2 could not determine the step(s) catalyzed by CYP90D2/D2, because CYP90D2/D2 functions redundantly with another gene, CYP90D3, and loss-of-function of CYP90D2/D2 have caused only limited defects in brassinosteroid biosynthesis[5]. Similarly, endogenous levels of brassinosteroid intermediates were obviously changed in the osdwarf4-1/d11-4 double mutant relative to osdwarf4-1 or d11 single mutants. Although brassinolide was not detected in either the wild-type or osdwarf4-1/d11-4, another bioactive brassinosteroid, castasterone (CS), was detected in the wild-type but not in osdwarf4-1/d11-4, confirming that osdwarf4-1/d11-4 is brassinosteroid-deficient (Fig. 2e). Levels of the other five intermediates downstream of C-22 hydroxylation—6-deoxocathasterone (6-DeoxoCT), 6-deoxoteasterone (6-DeoxoTE), 3-dehydro-6-deoxoteasterone (6-Deoxo3DT), 6-deoxotyphasterol (6-DeoxoTY) and 6-deoxocastasterone (6-DeoxoCS)—were also greatly reduced in osdwarf4-1/d11-4 (Fig. 2e). This indicates that both proteins are involved in C-22 hydroxylation. It is unclear why the upstream compound, campestanol, did not accumulate in osdwarf4-1/d11-4. Because A. thaliana CYP90B1/DWARF4 also catalyzes C-22 hydroxylation of other upstream compounds such as campesterol and (24R)-ergost-4-en-3-one[10], it is possible that campestanol is not the major substrate for CYP90B2/OsDWARF4 and CYP724B1/D11 in rice.

----Characterization of a field-grown osdwarf4-1 mutant

Because the harvest index (grain/(grain plus straw)) of rice is about 0.5, further increases in yield potential will have to involve increases in crop biomass driven by more net photosynthesis[11]. In rice, the contribution of lower leaves to photosynthesis is significant, even though their photosynthetic capacity is considerably less than that of upper leaves[12]. Erect leaves allow greater penetration of light to lower leaves, thereby optimizing canopy photosynthesis[13]. Therefore, the most likely explanation for the increase in above-ground biomass in osdwarf4-1 plots under dense planting conditions (more than 1.2 times that in wild-type under conventional planting condition) is that the shade of the upper leaves is minimized, and the lower leaves receive more light to drive higher rates of photosynthesis (Fig. 3 and Supplementary Table 2 online). No difference was observed in the heading date (flowering time) and the grain-filling period (time to maturity) between wild-type and osdwarf4-1 plants (data not shown). In the wild-type, the above-ground biomass was increased in response to higher fertilizer application under conventional planting (22.2 plants m-2), but was significantly decreased under dense planting with doubled fertilizer application owing to reduced photosynthesis caused by lodging (Fig. 3a). In contrast, the above-ground biomass of osdwarf4-1 was significantly (P < 0.01) increased by dense planting at each fertilizer rate. Consequently, the above-ground biomass in osdwarf4-1 plots under dense planting with standard and a 1.5-fold increase in fertilizer application was approx1.34 and 1.52 times that in wild-type plots under conventional cultivation conditions. The increased biomass depended mainly on accelerated formation of tillers, which have the ability to generate a panicle (panicle number, Fig. 3b). Both total and fertile grain numbers tended to increase in densely planted osdwarf4-1 plots (Fig. 3c), resulting in significantly increased grain yield even when the fertilizer application was not increased (for example, the osdwarf4-1 yield was 6.19 t ha-1 under dense planting with standard fertilizer application whereas the wild-type yield was 4.69 t ha-1 under conventional conditions; Fig. 3d). However, the increase in grain yield was not as much as that in above-ground biomass, because the harvest index of osdwarf4-1 (0.41) was slightly lower than that of the wild-type (0.44). A similar field test gave comparable results.

----Overexpression of OsDWARF4

Overexpression of DWARF in transgenic tomato increases plant height[14], and overexpression of DWARF4 in transgenic A. thaliana and tobacco increases vegetative growth and seed yield[15]. In rice, however, the role of brassinosteroid in regulating leaf angle has been known for over 40 years[16, 17]. Whereas brassinosteroid deficiency increased the erectness of leaves, the opposite phenotype (increased leaf angle) was observed in transgenic rice overexpressing OsDWARF4 (27.6° versus 10.0° in the wild-type, P < 0.01), and was inherited in their progeny (data not shown). Because inclined leaves are unfavorable for photosynthesis when plants are grown in a group, we have to carefully evaluate whether the overexpression of a brassinosteroid-biosynthesis gene in transgenic rice increases grain yield as is the case in transgenic plants overexpressing DWARF4.

===Conclusion===

More erect leaves improve not only light distribution but also the size and use of leaf nitrogen reservoirs for grain growth[13]. The erect leaf phenotype caused by brassinosteroid deficiency improved biomass production under field conditions, resulting in increased grain yield without the negative environmental effects associated with fertilizers. Recently identified rice homologs of A. thaliana BAS1 that encodes the brassinosteroid catabolic enzyme, CYP734A1 (ref. [18]). Overexpression of these rice homologs in transgenic rice successfully induced the brassinosteroid-deficient phenotypes (unpublished results). Although ideally, expression of these genes should be restricted to particular tissues and organs such as leaf laminae, it should be possible to generate erect leaf varieties by the introduction of a single transgene. In conclusion, engineering more erect leaves modulating brassinosteroid metabolism can be combined with dense planting to improve rice grain yields.

==Labs working on this gene==
Please input related labs here.

==References==
[1] Sakamoto, T. & Matsuoka, M. Generating high-yielding varieties by genetic manipulation of plant architecture. Curr. Opin. Biotech. 15, 144–147 (2004).
[2] Li, J. & Chory, J. A putative leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction. Cell 90, 929–938 (1997).
[3] Yamamuro, C. et al. Loss of function of a rice brassinosteroid insensitive1 homolog prevents internode elongation and bending of the lamina joint. Plant Cell 12, 1591–1606 (2000).
[4] Hong, Z. et al. Loss-of-function of a rice brassinosteroid biosynthetic enzyme, C-6 oxidase, prevents the organized arrangement and polar elongation of cells in the leaves and stem. Plant J. 32, 495–508 (2002).
[5] Hong, Z. et al. A rice brassinosteroid-deficient mutant, ebisu dwarf (d2), is caused by a loss of function of a new member of cytochrome P450. Plant Cell 15, 2900–2910 (2003).
[6]Sakamoto T;Morinaka Y;Ohnishi T;Sunohara H;Fujioka S;Ueguchi Tanaka M;Mizutani M;Sakata K;Takatsuto S;Yoshida S;Tanaka H;Kitano H;Matsuoka M Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice Nature Biotechnology, 2006, 24(1): 105-109.
[7] Choe, S. et al. The DWF4 gene of Arabidopsis encodes a cytochrome P450 that mediates multiple 22alpha-hydroxylation steps in brassinosteroid biosynthesis. Plant Cell 10, 231–243 (1998).
[8] Bancos, S. et al. Regulation of transcript levels of the Arabidopsis cytochrome P450 genes involved in brassinosteroid biosynthesis. Plant Physiol. 130, 504–513 (2002).
[9] Tanabe, S. et al. A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant, dwarf11, with reduced seed length. Plant Cell 17, 776–790 (2005).
[10] Fujioka, S., Takatsuto, S. & Yoshida, S. An early C-22 oxidation branch in brassinosteroid biosynthetic pathway. Plant Physiol. 130, 930–939 (2002).
[11] Mann, C.C. Crop scientists seek a new revolution. Science 283, 310–314 (1999).
[12] Horton, P. Prospects for crop improvement through the genetic manipulation of photosynthesis: morphological and biochemical aspects of light capture. J. Exp. Bot. 51, 475–485 (2000).
[13] Sinclair, T.R. & Sheehy, J.E. Erect leaves and photosynthesis in rice. Science 283, 1455 (1999).
[14] Bishop, G.J. et al. The tomato DWARF enzyme catalyses C-6 oxidation in brassinosteroid biosynthesis. Proc. Natl. Acad. Sci. USA 96, 1761–1766 (1999).
[15] Choe, S. et al. Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis. Plant J. 26, 573–582 (2001).
[16] Maeda, E. Rate of lamina inclination in excised rice leaves. Physiol. Plant. 18, 813–827 (1965).
[17] Wada, K. et al. Brassinolide and homobrassinolide promotion of lamina inclination of rice seedlings. Plant Cell Physiol. 22, 323–325 (1981).
[18] Neff, M.M. et al. BAS1: A gene regulating brassinosteroid levels and light responsiveness in Arabidopsis. Proc. Natl. Acad. Sci. USA 96, 15316–15323 (1999).

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0227700|
Description = Cytochrome P450 family protein|
Version = NM_001055982.2 GI:297600576 GeneID:4332134|
Length = 6529 bp|
Definition = Oryza sativa Japonica Group Os03g0227700, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:6765109..6771637|
CDS = 6765109..6765374,6765543..6765867,6765971..6766123,6768937..6769188,6769337..6769429<br>,6770222..6770300,6770398..6770504,6771392..6771637|
GCID = <gbrowseImage1>
name=NC_008396:6765109..6771637
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:6765109..6771637
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgccatgatggcgtccataaccagcgagctgctcttctttctccccttcatcctccttgccctgctcacgttctacaccaccaccgtggccaaatgccacggcgggcactggtggcgaggtgggacgacgccggcgaagaggaagcggatgaacctgccgcccggcgccgccgggtggccgctcgtcggcgagacgttcggctacctccgcgcccaccccgccacctccgtcggccgcttcatggagcagcacatcgcacggtacgggaagatataccggtcgagcctgttcggggagcggacggtggtgtcggcggacgcggggctcaaccggtacatcctgcagaacgaggggaggctgttcgagtgcagctacccgcgcagcatcggcggcatcctgggcaagtggtccatgctggtcctcgtcggggacccgcaccgcgagatgcgcgccatctccctcaacttcctctcctccgtccgcctccgcgccgtcctcctccccgaggtcgagcgccacaccctcctcgtcctccgcgcctggcccccttcctccaccttctccgctcagcaccaagccaagaagttcacgttcaacctgatggcgaagaacataatgagcatggacccgggggaggaagagacggagcggctgcggcgggagtacatcaccttcatgaagggcgtggtctccgcgccgctcaacctgcccgggacgccctactggaaggctctcaagtcgcgtgctgccattctcggagtaatagagaggaaaatggaagagcgggttgagaagctgagcaaggaggatgcaagcgtagagcaagacgatcttctcggatgggctctgaaacaatctaacctttcaaaagagcaaatcctggacctcttgctgagcttgctcttcgccgggcacgagacgtcgtccatggcgctcgccctcgccatcttcttccttgaaggctgccccaaggctgtccaagaactgagggaggagcatcttgggattgcaaggagacaaaggctaagaggggagtgcaaattgagctgggaagactacaaagagatggttttcacgcaatgtgtcataaacgagacgttgcggctaggaaacgtggtcaggttcctgcaccggaaggtcatcaaggacgtgcactacaagggttatgacattccaagcggatggaagatcctgccggtgttagccgcggtgcatctggactcgtccctgtacgaggacccccagcgcttcaatccctggagatggaagagtagcggatcatccggcggcttggctcagagcagcagcttcatgccgtacggcggcgggacgcggctgtgcgccgggtcggagctcgcgaagctggagatggccgtgttcttgcaccacctggtgctcaacttcaggtgggagctcgccgagccggaccaagccttcgtcttccccttcgtcgacttccccaagggccttcccattagggttcatagaattgcacaggatgatgagcaggagtaa</cdnaseq>|
AA = <aaseq>MAAMMASITSELLFFLPFILLALLTFYTTTVAKCHGGHWWRGGT TPAKRKRMNLPPGAAGWPLVGETFGYLRAHPATSVGRFMEQHIARYGKIYRSSLFGER TVVSADAGLNRYILQNEGRLFECSYPRSIGGILGKWSMLVLVGDPHREMRAISLNFLS SVRLRAVLLPEVERHTLLVLRAWPPSSTFSAQHQAKKFTFNLMAKNIMSMDPGEEETE RLRREYITFMKGVVSAPLNLPGTPYWKALKSRAAILGVIERKMEERVEKLSKEDASVE QDDLLGWALKQSNLSKEQILDLLLSLLFAGHETSSMALALAIFFLEGCPKAVQELREE HLGIARRQRLRGECKLSWEDYKEMVFTQCVINETLRLGNVVRFLHRKVIKDVHYKGYD IPSGWKILPVLAAVHLDSSLYEDPQRFNPWRWKSSGSSGGLAQSSSFMPYGGGTRLCA GSELAKLEMAVFLHHLVLNFRWELAEPDQAFVFPFVDFPKGLPIRVHRIAQDDEQE</aaseq>|
DNA = <dnaseqindica>1..266#435..759#863..1015#3829..4080#4229..4321#5114..5192#5290..5396#6284..6529#atggccgccatgatggcgtccataaccagcgagctgctcttctttctccccttcatcctccttgccctgctcacgttctacaccaccaccgtggccaaatgccacggcgggcactggtggcgaggtgggacgacgccggcgaagaggaagcggatgaacctgccgcccggcgccgccgggtggccgctcgtcggcgagacgttcggctacctccgcgcccaccccgccacctccgtcggccgcttcatggagcagcacatcgcacggtcggtggtttcattactcggtacacgcacacagctatataagcacgcgcactgctcgcctcgccgtgaagcaagcttagctagtagctaggatatcgatcgatcggtaataataggctgacggcgagctggggggtggcgtgcggttgcgtgcgtgcgtgcatgcaggtacgggaagatataccggtcgagcctgttcggggagcggacggtggtgtcggcggacgcggggctcaaccggtacatcctgcagaacgaggggaggctgttcgagtgcagctacccgcgcagcatcggcggcatcctgggcaagtggtccatgctggtcctcgtcggggacccgcaccgcgagatgcgcgccatctccctcaacttcctctcctccgtccgcctccgcgccgtcctcctccccgaggtcgagcgccacaccctcctcgtcctccgcgcctggcccccttcctccaccttctccgctcagcaccaagccaagaaggtacaacacacatcgtcctccattaagttgagcatgcatatatcaatcagctcgctctagcgatcaatatcaatgaaacgcgcgtttgcgtgcgtgcgtgcagttcacgttcaacctgatggcgaagaacataatgagcatggacccgggggaggaagagacggagcggctgcggcgggagtacatcaccttcatgaagggcgtggtctccgcgccgctcaacctgcccgggacgccctactggaaggctctcaaggtacgtactcgtagctactagctactccctccgtttcgaaatgtttgacgccgttgactttttatcacatatttgaccgttcgttttatttaaaaaaattaagtaattattaattattttcctatcatttgattcattgttaaatatatttttatgtaggcatataattttacatatctcacaaaagtttttaaataagacgaacggtcaaacatttgctaaaaagtcaacggtgtcaaatatttcgaaacggaggaagtagctagctaccaccccacccgtgcggattgatcgatcgatcgatgtccctctctgcctacacacaccctgcgccccggccgtacgcgcgcaaagtcgtgcgcgcatgagttttactccttccgtttcaaaaaaaaaacgacctagacaagtaatatgatgtaacctagaacaacgaatctatacaggagtatatccaaattcattgtcctagattacatcatatctttgtctatgttgccttttttaggaggagagagtactttacatatgagttatgaccggccggccaggttcctttcttcccctattccgtatgcatactataagcagctgcatataggcatatactactatacccagcttagctaaagcaaacaacttgcaacgacgatcatgtgcaggtccctgttgacagaggttagagatgtaaaccatttttgttgttttcattatctaaagagaaaacaagttactacgacgatcgagatgtacgcaaggggatcatatttatattggagaggagagagagatgatttgcgacttgtgagtgataagtgaatatatatactatatatttgtgtaggagtgacgatgacttccctgtcaatgttttgtttggtatgcacacggggggaaaagagtttagagaaaggaattggctagagagggccaggtttagtttctttaatttttgtttggtggttttgtgtgtagctagtccgcatgcatgatatatcatatatatgcatggcccatcattagatatatatacatgacggcagtgcacttattatatatatgtaggggcatatacaaggtgtatgtgtttagaattgtggatggtccaggaattgttgcagctagttctatactagcatagtgaattagtgatccatatcgagatcaggtccaatcggtcagctcgcacagcgggtcttttagtacgatttgttcggacaaattatcttgcctttgagataatcaacataggattgaaactgcatactagcaatagaccatagctatttggcaatatagaaagctaaaaaatgttgattgaaaggtgcgtgcatgcatgctagcagtatgtcttttaggccaggtccagtttttttttttttctcaaaggcagttgcttaaggtgaaattctagcagaggtcatatgaatatatttattatcttccaaacatgagccaaaacctatatatagttaactcaaaaaaccatgcattggtacttcttctgagtatgcatcctggggactgatcgatcatgcagattcttttctttctttttccctggggacgtactgatcatcactcatgtgagcataaaatgttgcctactttttactgcatgtgaggtcacactcaaatgatctttattgattgcctcagcatgcatgctgctagctttcacgatctctctccgatacacacaccctgtgctgtgctcaacagcatgcaacctggcaggtggaatttttaatctgttggaaagtaaatgaccaaccttcataatttggcatgattatatgctggggaatctatcatttatcatgattcaaccgctgaccaaactctttgccacacacccgaaccacattaatttcgttaagcggatgattagccagcaaccttcacagtttggttttaattagttaatgaaagggtgcttgtagttttattagctgaagagacactgcagtactcctactgagcgttctccttaattgggaaatagatctctgcagtactatcctttaactttaagtgaataacatgtagctaggtctcacacttatcaggcccacacatcagtgatccaagtcaaatggcaatacaccataggagattcttccactaattggctaacaaaaataaactgtgatcactgatcacagctgctaacactataacacattaatttgcatgtctcatgaaatgccagatgagttggattaattatctatcttatgttcagtgccgaggaatatacaaggaaacatcattggtatagttgtggagttctgagtgcattatctcacccagtacggtcaaattaatcctagtctccgtaaatatggttgtgtgcactcttagttaattagtgtagacccggaaattatactttgtcgagaatgttgttatccgtgtgttaagtttcaagtaccctgcaggcctataactaaacactgccaacatggatgtttaagaatgaattatacatacagcgcacttggaaaagaaaaggggtagctacccagctgaagaacacctctctctcatttgaaggtcgacacaatatgtgtcttttctatatcgaaaggaaacgacattttttctcttcaaaacagaaagctatgacaagagacataatcctagactagtaggcggttaggcaacaccatatctgaatttgataggtgtcccacatattttttttcttttcctgataagaaggaacttttcaccgtgagcttatttttttctgttctatgtcgacattagttccatgatgtttactgtgtatttttactgttcccctgcagtcgcgtgctgccattctcggagtaatagagaggaaaatggaagagcgggttgagaagctgagcaaggaggatgcaagcgtagagcaagacgatcttctcggatgggctctgaaacaatctaacctttcaaaagagcaaatcctggacctcttgctgagcttgctcttcgccgggcacgagacgtcgtccatggcgctcgccctcgccatcttcttccttgaaggctgccccaaggctgtccaagaactgagggtacagagaattacagattcgaaattaacatctactgtcactagctcagtgctacgtttaactgtagagcaacaattgcagaaagcgaaggcatcagaaatgacatgggaatcgattagttacactcacactgacgcgtgacatgcaggaggagcatcttgggattgcaaggagacaaaggctaagaggggagtgcaaattgagctgggaagactacaaagagatggttttcacgcaatgtgtaaggccccccctactttgttccttccgatccctcctccctaaacgctactaatataaatcggtgcaaaggctttgtgctagcgtagctcttgctgttttgtttggtcacttgcgtagagagatagcaacttcccgaaatgtcatgtacgaatggagactgaaagctctgcgaaataattgccgggccacgtactacagtacttacgctccctctcttcagtttggatcgatcgagctaagaaggcgcacgcatgtgtgcattgatcgatagagtcgatctgttctgccttgtaagctagccagctgaggttctttcgccttggaagtttctacagggtgaggcccatcgtcttgcaacgatccaaatgccatgacaagtacctgttgttgtaggcttccagtagcacggcctgcccccaagaaggtatctgaatcttctgcattgctgttcagacggcagagcagaacatgccgaaataccattccagtcagttgcagtatatgtgtgtggtggtatcagataaaggagcagagtaatttgaggccttagaaattcagatgagttgctcgacaattgtcgcctaccgatcgagaataagttgcatgcatgtatgtgatgtaattatgggtcttagtcagcaggatagtttagtcgctgcagtactgtagattgtagtacttgcgttcaaagcagacccaaaacagaacttgcattcaggaaacaaaacatgaaaaaagaaatatacagtacttggcactgatatttcttttgtttgggtccttattgcaggtcataaacgagacgttgcggctaggaaacgtggtcaggttcctgcaccggaaggtcatcaaggacgtgcactacaagggtgagtagctcgatttcactcttcttccctgaatcgacctctcctctatctgacagcagaagctaactgaactgaactgaactgaactgaactgaaggttatgacattccaagcggatggaagatcctgccggtgttagccgcggtgcatctggactcgtccctgtacgaggacccccagcgcttcaatccctggagatggaaggtcagtcgcagtaggattatcagtgaatctaatctattaatctcggtttaattgctgggattcaaggaaaagaaaatactgggacaagcgcggatgtcgctgttgggcagtgtctgtcaatcataagcgaaggggatcatgtcagaatctgcaaaagcagcacaggctaaacgcgtgcttgcctcgagcagcaatcatgcagagaggcaagctccgcgataggaacatgcatggtgaatcactgaacctgatatgaaagtgaatgctcagctcagctcgctcgctcgcgcgcgcatgtgctttctcatttcgccgtgttgcgtgctagctagtcaatggtgttcccacgaattgttttctcttctcatcacacaaagtcagtggtgaaaaccgaggtgcatgtgcggtaatggaacgggggattaggcggcgacgccgtgatggattatgcattcgtgcgagaaaaatccaggagcgttggtccaaatcgcaagcggatcaaaccgaaccaatcagtggacgattggagccctgcagtccatccatcacatctccattcttatccgctgcgactcacggacattagttgttgaggggtgaacgacgcatgtgtgtatgtatgctcgatcgatcgatcgaacaatggatctgtgtgtggtgtagttgccaacgccgacgagattatctgctcgtctgcatgcagccgcgaaaaagacatgtgtccggattatcctgcaggagattgagcaagataatctgctgcgcaaaaactgcagccaatcgatcattccatgacgcgaaaaaaaaaacaataattactactgctgatacgataacttcgcttcgaattgttgacacacaagtgttcttcttttgttcgattgcagagtagcggatcatccggcggcttggctcagagcagcagcttcatgccgtacggcggcgggacgcggctgtgcgccgggtcggagctcgcgaagctggagatggccgtgttcttgcaccacctggtgctcaacttcaggtgggagctcgccgagccggaccaagccttcgtcttccccttcgtcgacttccccaagggccttcccattagggttcatagaattgcacaggatgatgagcaggagtaa</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055982.2 RefSeq:Os03g0227700]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

Os03g0227700

2014-05-25T14:06:54Z

Liangdan2013:

Please input one-sentence summary here.

==Annotated Information==

Brassinosteroids influence both plant height and leaf erectness in rice[1]. Arabidopsis thaliana BRASSINOSTEROID INSENSITIVE1 (bri1) mutants, which are deficient in the brassinosteroid receptor, have a severe dwarf phenotype[2]. The rice mutants d61-1 and d61-2, which are weak mutant alleles of OsBRI1, a homolog of A. thaliana BRI1, have both a semi-dwarf phenotype and more erect leaves[3]. In rice, an erect leaf phenotype has also been associated with loss-of-function, brassinosteroid-deficient mutants such as brassinosteroid-deficient dwarf1 (brd1), which is defective in OsDWARF (the homolog of tomato CYP85A1/DWARF[4]), and ebisu dwarf (d2), which is defective in CYP90D2/D2 (ref. [5]). However, the grain yield of d61, brd1 and d2 mutants is decreased because of morphological alterations in their reproductive development.

===Function===

New cultivars with very erect leaves, which increase light capture for photosynthesis and nitrogen storage for grain filling, may have increased grain yields.The erect leaf phenotype of a rice brassinosteroid-deficient mutant, osdwarf4-1, is associated with enhanced grain yields under conditions of dense planting, even without extra fertilizer. Molecular and biochemical studies reveal that two different cytochrome P450s, CYP90B2/OsDWARF4 and CYP724B1/D11, function redundantly in C-22 hydroxylation, the rate-limiting step of brassinosteroid biosynthesis. Therefore, despite the central role of brassinosteroids in plant growth and development, mutation of OsDWARF4 alone causes only limited defects in brassinosteroid biosynthesis and plant morphology[6].

===Expression===
The affected gene, OsDWARF4, was identified by characterizing the site of insertion of the Tos17 retrotransposon.

----Molecular characterization of OsDWARF4 and OsDWARF4L1/D11

OsDWARF4 is a homolog of A. thaliana DWARF4, which encodes a cytochrome P450, CYP90B1 (Fig. 1a). CYP90B1/DWARF4 catalyzes C-22 hydroxylation, the rate-limiting step of brassinosteroid biosynthesis[7]. Only one DWARF4 homolog was identified in the rice genome. OsDWARF4 is located on the short arm of chromosome 3 (35 cM). Its predicted open reading frame consists of eight exons, and encodes a protein of 502 amino acids (Fig. 1b,c). OsDWARF4 is most closely related to DWARF4 (66.3% amino-acid identity; Fig. 1a). The structure of OsDWARF4 is similar to that of DWARF4 throughout its length: five domains found in cytochrome P450s—proline rich, A (also referred to as dioxygen binding), B (steroid binding), C and heme binding—are highly conserved (Fig. 1c). In wild-type rice, OsDWARF4 transcripts are most abundant in leaf blades and roots, but are also found in all other plant parts tested (Fig. 1d). Transcript levels were decreased by exogenous brassinolide treatment, and increased in brassinosteroid-insensitive d61-3 and brassinosteroid-deficient brd1-1 (Fig. 1e). These results suggest that OsDWARF4 expression is feedback regulated, as is that of A. thaliana brassinosteroid-biosynthesis genes[8].
OsDWARF4L1 was identified as an OsDWARF4-like gene by in silico screening of rice DNA databases. OsDWARF4L1 is located on the long arm of chromosome 4 (70.9 cM). A recent study demonstrated that a rice semi-dwarf and short-grain mutant, d11, has a loss-of-function mutation in the OsDWARF4L1 gene[9]. OsDWARF4L1/D11 shows the highest homology with A. thaliana CYP724A1, and is closely related to rice OsDWARF4 and A. thaliana DWARF4 (47.4%, 37.7% and 41.7% amino-acid identities, respectively; Fig. 1a). In wild-type rice, OsDWARF4L1/D11 transcripts were most abundant in vegetative shoot apices, leaf sheaths and roots, and found at more moderate levels in other organs (Fig. 1d). They were decreased by exogenous brassinolide treatment, and increased in d61-3 and brd1-1 (Fig. 1e). These results indicate that OsDWARF4L1/D11 expression is regulated by a feedback mechanism, as is that of OsDWARF4.

----Phenotypes of rice mutants defective in brassinosteroid biosynthesis

In osdwarf4-1, Tos17 insertion into exon 6 causes a premature termination in domain C (arrowhead in Fig. 1b,c). Because a premature stop codon before the heme-binding domain caused loss-of-function of A. thaliana DWARF4 (ref. [7]), we consider osdwarf4-1 to be a null allele. Unexpectedly, osdwarf4-1 plants show only weak morphological phenotypes, such as a slightly dwarfed stature and more erect leaves (Fig. 2a,b). Both of these phenotypes were complemented by the introduction of an entire OsDWARF4 gene (data not shown). These results seem inconsistent with the fact that rice d61-3 and brd1-1, which are loss-of-function mutants of OsBRI1 and OsDWARF respectively, display severe dwarfing and have malformed leaves (for example, see brd1-1 in Fig. 2a), and suggest functional redundancy in brassinosteroid biosynthesis in rice.
Because null alleles of d11, such as d11-4, also show a weak phenotype[9] (Fig. 2a),OsDWARF4L1/D11 and OsDWARF4 may function redundantly. To confirm this possibility, researcher produced double mutants by crossing homozygous osdwarf4-1 and d11-4 plants. Among the F2 population, 34 of 576 plants showed severe dwarfing and malformed leaves with tortuous leaf blades, indistinguishable from the brassinosteroid-deficient brd1-1 phenotype (Fig. 2a,c,d). This ratio fits the theoretical 9:3:3:1 (wild-type:osdwarf4-1:d11-4:osdwarf4-1/d11-4) segregation predicted by mendelian inheritance. PCR and sequence analyses confirmed that these plants were osdwarf4-1/d11-4 double mutants (data not shown).
Endogenous levels of brassinosteroid intermediates in brd1 clearly indicate that OsDWARF catalyzes C-6 oxidation[4]. However, quantification in d2 could not determine the step(s) catalyzed by CYP90D2/D2, because CYP90D2/D2 functions redundantly with another gene, CYP90D3, and loss-of-function of CYP90D2/D2 have caused only limited defects in brassinosteroid biosynthesis[5]. Similarly, endogenous levels of brassinosteroid intermediates were obviously changed in the osdwarf4-1/d11-4 double mutant relative to osdwarf4-1 or d11 single mutants. Although brassinolide was not detected in either the wild-type or osdwarf4-1/d11-4, another bioactive brassinosteroid, castasterone (CS), was detected in the wild-type but not in osdwarf4-1/d11-4, confirming that osdwarf4-1/d11-4 is brassinosteroid-deficient (Fig. 2e). Levels of the other five intermediates downstream of C-22 hydroxylation—6-deoxocathasterone (6-DeoxoCT), 6-deoxoteasterone (6-DeoxoTE), 3-dehydro-6-deoxoteasterone (6-Deoxo3DT), 6-deoxotyphasterol (6-DeoxoTY) and 6-deoxocastasterone (6-DeoxoCS)—were also greatly reduced in osdwarf4-1/d11-4 (Fig. 2e). This indicates that both proteins are involved in C-22 hydroxylation. It is unclear why the upstream compound, campestanol, did not accumulate in osdwarf4-1/d11-4. Because A. thaliana CYP90B1/DWARF4 also catalyzes C-22 hydroxylation of other upstream compounds such as campesterol and (24R)-ergost-4-en-3-one[10], it is possible that campestanol is not the major substrate for CYP90B2/OsDWARF4 and CYP724B1/D11 in rice.

----Characterization of a field-grown osdwarf4-1 mutant

Because the harvest index (grain/(grain plus straw)) of rice is about 0.5, further increases in yield potential will have to involve increases in crop biomass driven by more net photosynthesis[11]. In rice, the contribution of lower leaves to photosynthesis is significant, even though their photosynthetic capacity is considerably less than that of upper leaves[12]. Erect leaves allow greater penetration of light to lower leaves, thereby optimizing canopy photosynthesis[13]. Therefore, the most likely explanation for the increase in above-ground biomass in osdwarf4-1 plots under dense planting conditions (more than 1.2 times that in wild-type under conventional planting condition) is that the shade of the upper leaves is minimized, and the lower leaves receive more light to drive higher rates of photosynthesis (Fig. 3 and Supplementary Table 2 online). No difference was observed in the heading date (flowering time) and the grain-filling period (time to maturity) between wild-type and osdwarf4-1 plants (data not shown). In the wild-type, the above-ground biomass was increased in response to higher fertilizer application under conventional planting (22.2 plants m-2), but was significantly decreased under dense planting with doubled fertilizer application owing to reduced photosynthesis caused by lodging (Fig. 3a). In contrast, the above-ground biomass of osdwarf4-1 was significantly (P < 0.01) increased by dense planting at each fertilizer rate. Consequently, the above-ground biomass in osdwarf4-1 plots under dense planting with standard and a 1.5-fold increase in fertilizer application was approx1.34 and 1.52 times that in wild-type plots under conventional cultivation conditions. The increased biomass depended mainly on accelerated formation of tillers, which have the ability to generate a panicle (panicle number, Fig. 3b). Both total and fertile grain numbers tended to increase in densely planted osdwarf4-1 plots (Fig. 3c), resulting in significantly increased grain yield even when the fertilizer application was not increased (for example, the osdwarf4-1 yield was 6.19 t ha-1 under dense planting with standard fertilizer application whereas the wild-type yield was 4.69 t ha-1 under conventional conditions; Fig. 3d). However, the increase in grain yield was not as much as that in above-ground biomass, because the harvest index of osdwarf4-1 (0.41) was slightly lower than that of the wild-type (0.44). A similar field test gave comparable results.

----Overexpression of OsDWARF4

Overexpression of DWARF in transgenic tomato increases plant height[14], and overexpression of DWARF4 in transgenic A. thaliana and tobacco increases vegetative growth and seed yield[15]. In rice, however, the role of brassinosteroid in regulating leaf angle has been known for over 40 years[16, 17]. Whereas brassinosteroid deficiency increased the erectness of leaves, the opposite phenotype (increased leaf angle) was observed in transgenic rice overexpressing OsDWARF4 (27.6° versus 10.0° in the wild-type, P < 0.01), and was inherited in their progeny (data not shown). Because inclined leaves are unfavorable for photosynthesis when plants are grown in a group, we have to carefully evaluate whether the overexpression of a brassinosteroid-biosynthesis gene in transgenic rice increases grain yield as is the case in transgenic plants overexpressing DWARF4.

===Conclusion===

More erect leaves improve not only light distribution but also the size and use of leaf nitrogen reservoirs for grain growth[13]. The erect leaf phenotype caused by brassinosteroid deficiency improved biomass production under field conditions, resulting in increased grain yield without the negative environmental effects associated with fertilizers. Recently identified rice homologs of A. thaliana BAS1 that encodes the brassinosteroid catabolic enzyme, CYP734A1 (ref. [18]). Overexpression of these rice homologs in transgenic rice successfully induced the brassinosteroid-deficient phenotypes (unpublished results). Although ideally, expression of these genes should be restricted to particular tissues and organs such as leaf laminae, it should be possible to generate erect leaf varieties by the introduction of a single transgene. In conclusion, engineering more erect leaves modulating brassinosteroid metabolism can be combined with dense planting to improve rice grain yields.

==Labs working on this gene==
Please input related labs here.

==References==
[1] Sakamoto, T. & Matsuoka, M. Generating high-yielding varieties by genetic manipulation of plant architecture. Curr. Opin. Biotech. 15, 144–147 (2004).
[2] Li, J. & Chory, J. A putative leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction. Cell 90, 929–938 (1997).
[3] Yamamuro, C. et al. Loss of function of a rice brassinosteroid insensitive1 homolog prevents internode elongation and bending of the lamina joint. Plant Cell 12, 1591–1606 (2000).
[4] Hong, Z. et al. Loss-of-function of a rice brassinosteroid biosynthetic enzyme, C-6 oxidase, prevents the organized arrangement and polar elongation of cells in the leaves and stem. Plant J. 32, 495–508 (2002).
[5] Hong, Z. et al. A rice brassinosteroid-deficient mutant, ebisu dwarf (d2), is caused by a loss of function of a new member of cytochrome P450. Plant Cell 15, 2900–2910 (2003).
[6]Sakamoto T;Morinaka Y;Ohnishi T;Sunohara H;Fujioka S;Ueguchi Tanaka M;Mizutani M;Sakata K;Takatsuto S;Yoshida S;Tanaka H;Kitano H;Matsuoka M Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice Nature Biotechnology, 2006, 24(1): 105-109.
[7] Choe, S. et al. The DWF4 gene of Arabidopsis encodes a cytochrome P450 that mediates multiple 22alpha-hydroxylation steps in brassinosteroid biosynthesis. Plant Cell 10, 231–243 (1998).
[8] Bancos, S. et al. Regulation of transcript levels of the Arabidopsis cytochrome P450 genes involved in brassinosteroid biosynthesis. Plant Physiol. 130, 504–513 (2002).
[9] Tanabe, S. et al. A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant, dwarf11, with reduced seed length. Plant Cell 17, 776–790 (2005).
[10] Fujioka, S., Takatsuto, S. & Yoshida, S. An early C-22 oxidation branch in brassinosteroid biosynthetic pathway. Plant Physiol. 130, 930–939 (2002).
[11] Mann, C.C. Crop scientists seek a new revolution. Science 283, 310–314 (1999).
[12] Horton, P. Prospects for crop improvement through the genetic manipulation of photosynthesis: morphological and biochemical aspects of light capture. J. Exp. Bot. 51, 475–485 (2000).
[13] Sinclair, T.R. & Sheehy, J.E. Erect leaves and photosynthesis in rice. Science 283, 1455 (1999).
[14] Bishop, G.J. et al. The tomato DWARF enzyme catalyses C-6 oxidation in brassinosteroid biosynthesis. Proc. Natl. Acad. Sci. USA 96, 1761–1766 (1999).
[15] Choe, S. et al. Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis. Plant J. 26, 573–582 (2001).
[16] Maeda, E. Rate of lamina inclination in excised rice leaves. Physiol. Plant. 18, 813–827 (1965).
[17] Wada, K. et al. Brassinolide and homobrassinolide promotion of lamina inclination of rice seedlings. Plant Cell Physiol. 22, 323–325 (1981).
[18] Neff, M.M. et al. BAS1: A gene regulating brassinosteroid levels and light responsiveness in Arabidopsis. Proc. Natl. Acad. Sci. USA 96, 15316–15323 (1999).

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0227700|
Description = Cytochrome P450 family protein|
Version = NM_001055982.2 GI:297600576 GeneID:4332134|
Length = 6529 bp|
Definition = Oryza sativa Japonica Group Os03g0227700, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:6765109..6771637|
CDS = 6765109..6765374,6765543..6765867,6765971..6766123,6768937..6769188,6769337..6769429<br>,6770222..6770300,6770398..6770504,6771392..6771637|
GCID = <gbrowseImage1>
name=NC_008396:6765109..6771637
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:6765109..6771637
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgccatgatggcgtccataaccagcgagctgctcttctttctccccttcatcctccttgccctgctcacgttctacaccaccaccgtggccaaatgccacggcgggcactggtggcgaggtgggacgacgccggcgaagaggaagcggatgaacctgccgcccggcgccgccgggtggccgctcgtcggcgagacgttcggctacctccgcgcccaccccgccacctccgtcggccgcttcatggagcagcacatcgcacggtacgggaagatataccggtcgagcctgttcggggagcggacggtggtgtcggcggacgcggggctcaaccggtacatcctgcagaacgaggggaggctgttcgagtgcagctacccgcgcagcatcggcggcatcctgggcaagtggtccatgctggtcctcgtcggggacccgcaccgcgagatgcgcgccatctccctcaacttcctctcctccgtccgcctccgcgccgtcctcctccccgaggtcgagcgccacaccctcctcgtcctccgcgcctggcccccttcctccaccttctccgctcagcaccaagccaagaagttcacgttcaacctgatggcgaagaacataatgagcatggacccgggggaggaagagacggagcggctgcggcgggagtacatcaccttcatgaagggcgtggtctccgcgccgctcaacctgcccgggacgccctactggaaggctctcaagtcgcgtgctgccattctcggagtaatagagaggaaaatggaagagcgggttgagaagctgagcaaggaggatgcaagcgtagagcaagacgatcttctcggatgggctctgaaacaatctaacctttcaaaagagcaaatcctggacctcttgctgagcttgctcttcgccgggcacgagacgtcgtccatggcgctcgccctcgccatcttcttccttgaaggctgccccaaggctgtccaagaactgagggaggagcatcttgggattgcaaggagacaaaggctaagaggggagtgcaaattgagctgggaagactacaaagagatggttttcacgcaatgtgtcataaacgagacgttgcggctaggaaacgtggtcaggttcctgcaccggaaggtcatcaaggacgtgcactacaagggttatgacattccaagcggatggaagatcctgccggtgttagccgcggtgcatctggactcgtccctgtacgaggacccccagcgcttcaatccctggagatggaagagtagcggatcatccggcggcttggctcagagcagcagcttcatgccgtacggcggcgggacgcggctgtgcgccgggtcggagctcgcgaagctggagatggccgtgttcttgcaccacctggtgctcaacttcaggtgggagctcgccgagccggaccaagccttcgtcttccccttcgtcgacttccccaagggccttcccattagggttcatagaattgcacaggatgatgagcaggagtaa</cdnaseq>|
AA = <aaseq>MAAMMASITSELLFFLPFILLALLTFYTTTVAKCHGGHWWRGGT TPAKRKRMNLPPGAAGWPLVGETFGYLRAHPATSVGRFMEQHIARYGKIYRSSLFGER TVVSADAGLNRYILQNEGRLFECSYPRSIGGILGKWSMLVLVGDPHREMRAISLNFLS SVRLRAVLLPEVERHTLLVLRAWPPSSTFSAQHQAKKFTFNLMAKNIMSMDPGEEETE RLRREYITFMKGVVSAPLNLPGTPYWKALKSRAAILGVIERKMEERVEKLSKEDASVE QDDLLGWALKQSNLSKEQILDLLLSLLFAGHETSSMALALAIFFLEGCPKAVQELREE HLGIARRQRLRGECKLSWEDYKEMVFTQCVINETLRLGNVVRFLHRKVIKDVHYKGYD IPSGWKILPVLAAVHLDSSLYEDPQRFNPWRWKSSGSSGGLAQSSSFMPYGGGTRLCA GSELAKLEMAVFLHHLVLNFRWELAEPDQAFVFPFVDFPKGLPIRVHRIAQDDEQE</aaseq>|
DNA = <dnaseqindica>1..266#435..759#863..1015#3829..4080#4229..4321#5114..5192#5290..5396#6284..6529#atggccgccatgatggcgtccataaccagcgagctgctcttctttctccccttcatcctccttgccctgctcacgttctacaccaccaccgtggccaaatgccacggcgggcactggtggcgaggtgggacgacgccggcgaagaggaagcggatgaacctgccgcccggcgccgccgggtggccgctcgtcggcgagacgttcggctacctccgcgcccaccccgccacctccgtcggccgcttcatggagcagcacatcgcacggtcggtggtttcattactcggtacacgcacacagctatataagcacgcgcactgctcgcctcgccgtgaagcaagcttagctagtagctaggatatcgatcgatcggtaataataggctgacggcgagctggggggtggcgtgcggttgcgtgcgtgcgtgcatgcaggtacgggaagatataccggtcgagcctgttcggggagcggacggtggtgtcggcggacgcggggctcaaccggtacatcctgcagaacgaggggaggctgttcgagtgcagctacccgcgcagcatcggcggcatcctgggcaagtggtccatgctggtcctcgtcggggacccgcaccgcgagatgcgcgccatctccctcaacttcctctcctccgtccgcctccgcgccgtcctcctccccgaggtcgagcgccacaccctcctcgtcctccgcgcctggcccccttcctccaccttctccgctcagcaccaagccaagaaggtacaacacacatcgtcctccattaagttgagcatgcatatatcaatcagctcgctctagcgatcaatatcaatgaaacgcgcgtttgcgtgcgtgcgtgcagttcacgttcaacctgatggcgaagaacataatgagcatggacccgggggaggaagagacggagcggctgcggcgggagtacatcaccttcatgaagggcgtggtctccgcgccgctcaacctgcccgggacgccctactggaaggctctcaaggtacgtactcgtagctactagctactccctccgtttcgaaatgtttgacgccgttgactttttatcacatatttgaccgttcgttttatttaaaaaaattaagtaattattaattattttcctatcatttgattcattgttaaatatatttttatgtaggcatataattttacatatctcacaaaagtttttaaataagacgaacggtcaaacatttgctaaaaagtcaacggtgtcaaatatttcgaaacggaggaagtagctagctaccaccccacccgtgcggattgatcgatcgatcgatgtccctctctgcctacacacaccctgcgccccggccgtacgcgcgcaaagtcgtgcgcgcatgagttttactccttccgtttcaaaaaaaaaacgacctagacaagtaatatgatgtaacctagaacaacgaatctatacaggagtatatccaaattcattgtcctagattacatcatatctttgtctatgttgccttttttaggaggagagagtactttacatatgagttatgaccggccggccaggttcctttcttcccctattccgtatgcatactataagcagctgcatataggcatatactactatacccagcttagctaaagcaaacaacttgcaacgacgatcatgtgcaggtccctgttgacagaggttagagatgtaaaccatttttgttgttttcattatctaaagagaaaacaagttactacgacgatcgagatgtacgcaaggggatcatatttatattggagaggagagagagatgatttgcgacttgtgagtgataagtgaatatatatactatatatttgtgtaggagtgacgatgacttccctgtcaatgttttgtttggtatgcacacggggggaaaagagtttagagaaaggaattggctagagagggccaggtttagtttctttaatttttgtttggtggttttgtgtgtagctagtccgcatgcatgatatatcatatatatgcatggcccatcattagatatatatacatgacggcagtgcacttattatatatatgtaggggcatatacaaggtgtatgtgtttagaattgtggatggtccaggaattgttgcagctagttctatactagcatagtgaattagtgatccatatcgagatcaggtccaatcggtcagctcgcacagcgggtcttttagtacgatttgttcggacaaattatcttgcctttgagataatcaacataggattgaaactgcatactagcaatagaccatagctatttggcaatatagaaagctaaaaaatgttgattgaaaggtgcgtgcatgcatgctagcagtatgtcttttaggccaggtccagtttttttttttttctcaaaggcagttgcttaaggtgaaattctagcagaggtcatatgaatatatttattatcttccaaacatgagccaaaacctatatatagttaactcaaaaaaccatgcattggtacttcttctgagtatgcatcctggggactgatcgatcatgcagattcttttctttctttttccctggggacgtactgatcatcactcatgtgagcataaaatgttgcctactttttactgcatgtgaggtcacactcaaatgatctttattgattgcctcagcatgcatgctgctagctttcacgatctctctccgatacacacaccctgtgctgtgctcaacagcatgcaacctggcaggtggaatttttaatctgttggaaagtaaatgaccaaccttcataatttggcatgattatatgctggggaatctatcatttatcatgattcaaccgctgaccaaactctttgccacacacccgaaccacattaatttcgttaagcggatgattagccagcaaccttcacagtttggttttaattagttaatgaaagggtgcttgtagttttattagctgaagagacactgcagtactcctactgagcgttctccttaattgggaaatagatctctgcagtactatcctttaactttaagtgaataacatgtagctaggtctcacacttatcaggcccacacatcagtgatccaagtcaaatggcaatacaccataggagattcttccactaattggctaacaaaaataaactgtgatcactgatcacagctgctaacactataacacattaatttgcatgtctcatgaaatgccagatgagttggattaattatctatcttatgttcagtgccgaggaatatacaaggaaacatcattggtatagttgtggagttctgagtgcattatctcacccagtacggtcaaattaatcctagtctccgtaaatatggttgtgtgcactcttagttaattagtgtagacccggaaattatactttgtcgagaatgttgttatccgtgtgttaagtttcaagtaccctgcaggcctataactaaacactgccaacatggatgtttaagaatgaattatacatacagcgcacttggaaaagaaaaggggtagctacccagctgaagaacacctctctctcatttgaaggtcgacacaatatgtgtcttttctatatcgaaaggaaacgacattttttctcttcaaaacagaaagctatgacaagagacataatcctagactagtaggcggttaggcaacaccatatctgaatttgataggtgtcccacatattttttttcttttcctgataagaaggaacttttcaccgtgagcttatttttttctgttctatgtcgacattagttccatgatgtttactgtgtatttttactgttcccctgcagtcgcgtgctgccattctcggagtaatagagaggaaaatggaagagcgggttgagaagctgagcaaggaggatgcaagcgtagagcaagacgatcttctcggatgggctctgaaacaatctaacctttcaaaagagcaaatcctggacctcttgctgagcttgctcttcgccgggcacgagacgtcgtccatggcgctcgccctcgccatcttcttccttgaaggctgccccaaggctgtccaagaactgagggtacagagaattacagattcgaaattaacatctactgtcactagctcagtgctacgtttaactgtagagcaacaattgcagaaagcgaaggcatcagaaatgacatgggaatcgattagttacactcacactgacgcgtgacatgcaggaggagcatcttgggattgcaaggagacaaaggctaagaggggagtgcaaattgagctgggaagactacaaagagatggttttcacgcaatgtgtaaggccccccctactttgttccttccgatccctcctccctaaacgctactaatataaatcggtgcaaaggctttgtgctagcgtagctcttgctgttttgtttggtcacttgcgtagagagatagcaacttcccgaaatgtcatgtacgaatggagactgaaagctctgcgaaataattgccgggccacgtactacagtacttacgctccctctcttcagtttggatcgatcgagctaagaaggcgcacgcatgtgtgcattgatcgatagagtcgatctgttctgccttgtaagctagccagctgaggttctttcgccttggaagtttctacagggtgaggcccatcgtcttgcaacgatccaaatgccatgacaagtacctgttgttgtaggcttccagtagcacggcctgcccccaagaaggtatctgaatcttctgcattgctgttcagacggcagagcagaacatgccgaaataccattccagtcagttgcagtatatgtgtgtggtggtatcagataaaggagcagagtaatttgaggccttagaaattcagatgagttgctcgacaattgtcgcctaccgatcgagaataagttgcatgcatgtatgtgatgtaattatgggtcttagtcagcaggatagtttagtcgctgcagtactgtagattgtagtacttgcgttcaaagcagacccaaaacagaacttgcattcaggaaacaaaacatgaaaaaagaaatatacagtacttggcactgatatttcttttgtttgggtccttattgcaggtcataaacgagacgttgcggctaggaaacgtggtcaggttcctgcaccggaaggtcatcaaggacgtgcactacaagggtgagtagctcgatttcactcttcttccctgaatcgacctctcctctatctgacagcagaagctaactgaactgaactgaactgaactgaactgaaggttatgacattccaagcggatggaagatcctgccggtgttagccgcggtgcatctggactcgtccctgtacgaggacccccagcgcttcaatccctggagatggaaggtcagtcgcagtaggattatcagtgaatctaatctattaatctcggtttaattgctgggattcaaggaaaagaaaatactgggacaagcgcggatgtcgctgttgggcagtgtctgtcaatcataagcgaaggggatcatgtcagaatctgcaaaagcagcacaggctaaacgcgtgcttgcctcgagcagcaatcatgcagagaggcaagctccgcgataggaacatgcatggtgaatcactgaacctgatatgaaagtgaatgctcagctcagctcgctcgctcgcgcgcgcatgtgctttctcatttcgccgtgttgcgtgctagctagtcaatggtgttcccacgaattgttttctcttctcatcacacaaagtcagtggtgaaaaccgaggtgcatgtgcggtaatggaacgggggattaggcggcgacgccgtgatggattatgcattcgtgcgagaaaaatccaggagcgttggtccaaatcgcaagcggatcaaaccgaaccaatcagtggacgattggagccctgcagtccatccatcacatctccattcttatccgctgcgactcacggacattagttgttgaggggtgaacgacgcatgtgtgtatgtatgctcgatcgatcgatcgaacaatggatctgtgtgtggtgtagttgccaacgccgacgagattatctgctcgtctgcatgcagccgcgaaaaagacatgtgtccggattatcctgcaggagattgagcaagataatctgctgcgcaaaaactgcagccaatcgatcattccatgacgcgaaaaaaaaaacaataattactactgctgatacgataacttcgcttcgaattgttgacacacaagtgttcttcttttgttcgattgcagagtagcggatcatccggcggcttggctcagagcagcagcttcatgccgtacggcggcgggacgcggctgtgcgccgggtcggagctcgcgaagctggagatggccgtgttcttgcaccacctggtgctcaacttcaggtgggagctcgccgagccggaccaagccttcgtcttccccttcgtcgacttccccaagggccttcccattagggttcatagaattgcacaggatgatgagcaggagtaa</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055982.2 RefSeq:Os03g0227700]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

Os01g0782100

2014-05-24T16:59:49Z

Liangdan2013: /* Expression */

Please input one-sentence summary here.
This rice blast resistance genes was found by two Japanese scholars Imbe and Matsumoto in 1985, they called this gene Pi-sh,this gene has the moderate resistance to the blast fungus strain Kyu77-07A in rice variety Shin-2.this gene has been characterized by using the method of classical genetic.And this gene is linking to pi-t.
==Annotated Information==
===Function===
The genes are accounting for the moderate resistance of the rice variety Shin 2 and some other varieties to the blast fungus strain Kyu 77-07A. Aichi Asahi (Pi-a) and carried out in the present study. Yashiro-mochi (Pi-ta), were found to be susceptible to the strain, while Ishikari Shirol*.e (Pi-i), Kanto 51 (Pi-k), Tsuyuake (Pi-k'!b), Fukunishiki (Pi-z) and Toride I (Pi-zt) were resistant, and Shin 2 (Pi-ks) and Pi N0.4 (Pi-ta2) moderately resistant. Therefore, Kyu 77-07A was classified as one of the strains belonging to race 102. Howver, the variety Reiho with the resistance gene Pi-ta2 was susceptible to this strain. Based on the knovn facts mentioned above, the objectives 0L the present study were' to determinA- whether Pi-ks is the gene responsible for the moderate resistance of Shin 2' to Kyu 77-07A along with the reason for the different reactions to Kyu 77-07A betweerL Pi N0.4 and Reiho which both have the same resistance gene Pi-ta2. For the gene analysis, the F2 or F3 plants of the crosses were inoculated with Kyu 77-07A and three other fungus strains using the spraying rnethod when the plants were at the four- to five-1eaf stage. Two varieties, Mineyutaka. and Saikai 155 belonging to the Shin 2 type varieties, were used as the representative parents of the varieties susceptible to Kyu 77-07A for the crosses.

===Expression===
To identify novel factors required for R gene-mediated resistance in rice, we used rice insertional mutant lines, induced by the endogenous retrotransposon Tos17, in a forward screen involving the rice blast fungus Magnaporthe oryzae. We inoculated 41,119 mutant lines with the fungus using a high throughput procedure, and identified 86 mutant lines with diminished resistance. A genome analysis revealed that 72 of the 86 lines contained mutations in a gene encoding a nucleotide binding site (NB) and leucine rich repeat (LRR) domain-containing (NLR) protein. A genetic complementation analysis and a pathogenesis assay demonstrated that this NLR gene encodes Pish, which confers resistance against races of M. oryzae containing avrPish. The other 14 lines have intact copies of the Pish gene, suggesting that they may contain mutations in the signaling components downstream of Pish. The genome analysis indicated that Pish and its neighboring three NLR genes are high similar to one another and are tandemly located. An in silico analysis of a Tos17 flanking sequence database revealed that this region is a "hot spot" for insertion. Intriguingly, the insertion sites are not distributed evenly among these four NLR genes, despite their similarity at the sequence and expression levels.
In this work we isolated the R gene Pish, and identified several other mutants involved in the signal transduction required for Pish-mediated resistance. These results indicate that our genetic approach is efficient and useful for unveiling novel aspects of defense signaling in rice. Furthermore, our data provide experimental evidence that R gene clusters have the potential to be highly preferred targets for transposable element insertions in plant genomes. Based on this finding, a possible mechanism underlying the high variability of R genes is discussed.

===Evolution===
A study was conducted to investigate the relationship between phenotype of blast resistance and resistance gene analog polymorphism of rice, and search molecule hereditary basis of broad spectrum and durable resistance.
【Method】 Comparison of clustering analysis was investigated using spectrum of resistance to blast and polymorphism of resistance gene analog (RGA) in 25 varieties for blast resistance identification and 20 varieties lines).
【Result】 The resistance spectrum clustering analysis showed that the 45 varieties (lines) could be divided into group A and group B with the genetic similarity coefficient of 0.450. Group A and group B could be divided into subclassⅰ, subclass , ⅱ subclass , ⅲ subclass ⅳ, respectively, with 0.618 genetic similarity coefficient. The RGA-PCR clustering analysis showed that proposed the 45 varieties (lines) could be divided into group Ⅰ and group Ⅱ which clearly inclined the Indica-japonica differentiation with 0.620 genetic similarity coefficient. Group Ⅰ could be divided into six subclasses and group Ⅱ could be divided into seven subclasses with 0.783 genetic similarity coefficient. The resistance spectrum clustering analysis showed that some varieties with similar resistance spectrum could finely fall into the same group, while the RGA-PCR clustering analysis showed that some varieties with the similar genetic background could fall into the same group. For some varieties with low resistance frequency or high resistance frequency, there was a better corresponding relationship between the resistance spectrum clustering and the RGA-PCR clustering. General comparison of clustering analysis showed that here was no parallelism relationship between group and group in two different types of the clustering.
【Conclusion】 It could more accurately reflect their enetic background to test resistance to single strain and analysis on RGA polymorphism for resistance parents, and avoid applying the same source of resistance gain and again, and enrich rice resistance germplasm, and breed durable resistance varieties.

You can also add sub-section(s) at will.

==Labs working on this gene==
1.Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Ibaraki, 305-8602，Japan.
2.International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines;
3.Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, Japan;
4.Indonesian Center for Rice Research (ICRR), JL. Raya Muara No. 25A Ciapus Bogor, Subang, West Java, Indonesia;
5.Agricultural Genetics Institute,Conhue, Tuliem, Hanoi, Vietnam;
6.Faculty of Agricultural Sciences, University of Burundi, BP 2940 Bujumbura, Burundi;

==References==
1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta
Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.)
Plant Breeding, 2010, 129(6): 670-675
2. Akira Takahashi;Nagao Hayashi;Akio Miyao;Hirohiko Hirochika
Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging
BMC Plant Biology, 2010, 10: 175
3. Tokio IMBE;Shohei MATSUMOTO
Inheritance of Resrstance of Rice Vanetles to the Blast Fungus Strains Virulent to the Variety "Reiho"
Japanese Journal of Breeding, 1985, 35(0): 332-339.
4.鄂志国, 张丽靖, 焦桂爱, 等. 稻瘟病抗性基因的鉴定及利用进展[J]. 中国水稻科学, 2008, 22(5): 533-540.
5.杨勤忠, 林菲, 冯淑杰, 等. 水稻稻瘟病抗性基因的分子定位及克隆研究进展[J]. 中国农业科学, 2009, 42(5): 1601-1615.
6.任鄄胜, 肖培村, 陈勇, 等. 水稻品种稻瘟病抗性和抗病基因同源序列多态性分析[J]. 中国农业科学, 2009, 42(1): 1-9.
==Structured Information==
{{JaponicaGene|
GeneName = Os01g0782100|
Description = Similar to NBS-LRR protein (Fragment)|
Version = NM_001185663.1 GI:297720460 GeneID:9271927|
Length = 8696 bp|
Definition = Oryza sativa Japonica Group Os01g0782100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:34892692..34901387|
CDS = 34896973..34900845|
GCID = <gbrowseImage1>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaatgtgtttacaatgacaagagggtacaagaacattttgacgtcaggatatgggtctgcatctcacgcaaacttgatgttcatcgccatacacgtgagattatcgagtcagcaacaaatggagagtgccctcgtgtggacaatcttgatactctccagtgcagattgaaggacataatgcaaaaatcagagaaattcctgcttgtgttggatgacgtctggtttgatgaatccgtcaatgagagggaatgggaccagttgcttgatcctcttgtttctcagcaggaggggagcagagttttggtgacttctagacgagatgtacttccagctgctcttcactgtaaggatgttgttcatttggaaaacatggaagatgccgagttcttggctctattcaaataccatgctttctctggaacagaaatcagaaatccacagttgcatgcacggctagaagaggtcgcagagaagattgctaaaaggttaggacaatcgcctttagcagcaaggacagtaggctcccagctgagcaggaataaggatattgccatatggaaaagtgctctaaatattgaaaacttaagtgagcccatgaaagctctgttgtggagttataataaattagattcacgtctgcagagatgttttctgtactgcagcttatttccaaaaggtcacaagtataaaattgatgagatggttgacctttgggtggcagaggggctagttgattcacgcaaccagggggataagagaattgaagatattggcagggattacttcaatgagatggtgtcaggatctttctttcaaccagtttctgaaagatatatgggtacatggtacattatgcatgatctgcttcatgatttggcagagtcactgactaaagaagactgcttcagattagaagatgatggggtgaaagagataccagccactgttcgacatctatctatttgtgttgacagtatgaaattccataagcaaaagatctgcaagctacgttatttacgcactgttatctgcattgaccctctcatggatgacggagatgatatttttaatcagctattgaagaatctgaagaagctacgtgtactacatttgtcgttttacaacagtagcagcttgcctgaatgtattggtgagctgaagcaccttcgatatttgagtatcatcagcacattgatttctgaactgccaagatcattgtgtactcttttccacttggagctacttcatttgaatgacaaggtcaagaatttgccggacagactctgtaatttaagaaagttacgacgtcttgaagcatatgatgacagaaacagaatgtataaattgtatagagcagctctccctcaaattccttacataggcaagctatctttgctgcaagatattgatggattttgcgtgcaaaagcagaagggatatgagttgcgacaactgagggacatgaacaagctcggtggtaatttgcgtgtcgttaatcttgaaaatgtaactggaaaggatgaagcctcagagtcgaagttgcatcagaaaactcatcttagaggtttgcatctctcctggaatgatgtggatgacatggatgtttcacatttggagattctagaaggtttgaggccgccatctcaattggaggaccttacaatcgaaggttacaaatccaccatgtacccaagctggttacttgatggatcctattttgagaatctagagtcttttacgcttgctaattgctgtgtgataggaagcctaccacccaataccgagatctttaggcactgtatgacacttacccttgagaatgtcccgaatatgaagacactaccttttcttccagaaggtctcacaagcctatcaattgaggggtgcccactgcttgtgttcactactaataacgatgaactggaacaccatgattatagggagagcatcacgagggcaaacaacctggaaacacaacttgttttgatatgggaagcgaattccgattcagatattaggagcacattgtcgtccgaacactcatccatgaagaagttgacggaattgatggatactgatatgtcgggaaatcttcaaaccattgaaagcgctttagaaatagagagagatgaagccttggttaaagaagatatcatcaaagtatggctctgttgccacgaggagaggatgagattcatttattcaaggaaggctgggttgccgttggttctaccatctggactatgcgtactctctctttcctcctgtagtattacagatggagctttagctatttgccttggtgggcttacttcacttagaaatttgttcttaacagagattatgactttaactacgcttccaccggaagaggtcttccaacatttgggcaatcttagatacttggtcattcgttcctgctggtgtctcagatcattcgggggcttgcgatctgctacctctctttcagaaataagattattttcctgtccttctttacagttggcacgtggcgcagaatttatgcaaatgtcccttgagaagctatgtgtatacaactgtgtgctttcagctgacttcttctgcggtgactggccacatctgaataatattgggttatgtgggtgtagaagctctgcgtccttgtatgttggtgatcttacctccctcaaatcattctcactatatcatttgccagatttatgcgtgctggaaggtttgtcttacctacagcttcaccacgtgcatttgatagatgttccaaagcttactaccgagagcatctcacagttccgcgtacagcgttcgctctatattagcagttccgttatgctcaaccacatgatctccgctgaaggtttcaaagttccagggtttctctctcttgaaagctgcaagaagccatccgtttcattcgaagaatctgcaaatttcacatccgtcaagtgcctgaggttatgtaattgtgaaatgaggtcactaccaggaaatatgaagtgcctatccagtctaacgaaactcgatatctatgactgccccaacataacatctctaccagatctgccgtcctccctccagcatatatgtatatggggttgtgagcttttgaagaagagctgcagggcacctgatggagaaagctggccaaagattgcgcatatccgctggaaggaattcagatga</cdnaseq>|
AA = <aaseq>MAEVVLAGLRLAATPICVKLLCNASTCLGVDMTRELHELETIII PQFELVIEAAEKGNHRAKLDRWLRELKQAFYNAEDLLDEHEYNILKCKAKHKDSLVKD STQVHDSSISNILKQPMRAVSSRMSNLRPENRKILCQLNELKTMLEKAKEFRELIHLP AGNSLEGPSVPTIVVPVVTSLLPPRVFGRNMDRDRIIHLLTKPMATVSSSVGYSGLAI VAHGGAGKSTLAQCVYNDKRVQEHFDVRIWVCISRKLDVHRHTREIIESATNGECPRV DNLDTLQCRLKDIMQKSEKFLLVLDDVWFDESVNEREWDQLLDPLVSQQEGSRVLVTS RRDVLPAALHCKDVVHLENMEDAEFLALFKYHAFSGTEIRNPQLHARLEEVAEKIAKR LGQSPLAARTVGSQLSRNKDIAIWKSALNIENLSEPMKALLWSYNKLDSRLQRCFLYC SLFPKGHKYKIDEMVDLWVAEGLVDSRNQGDKRIEDIGRDYFNEMVSGSFFQPVSERY MGTWYIMHDLLHDLAESLTKEDCFRLEDDGVKEIPATVRHLSICVDSMKFHKQKICKL RYLRTVICIDPLMDDGDDIFNQLLKNLKKLRVLHLSFYNSSSLPECIGELKHLRYLSI ISTLISELPRSLCTLFHLELLHLNDKVKNLPDRLCNLRKLRRLEAYDDRNRMYKLYRA ALPQIPYIGKLSLLQDIDGFCVQKQKGYELRQLRDMNKLGGNLRVVNLENVTGKDEAS ESKLHQKTHLRGLHLSWNDVDDMDVSHLEILEGLRPPSQLEDLTIEGYKSTMYPSWLL DGSYFENLESFTLANCCVIGSLPPNTEIFRHCMTLTLENVPNMKTLPFLPEGLTSLSI EGCPLLVFTTNNDELEHHDYRESITRANNLETQLVLIWEANSDSDIRSTLSSEHSSMK KLTELMDTDMSGNLQTIESALEIERDEALVKEDIIKVWLCCHEERMRFIYSRKAGLPL VLPSGLCVLSLSSCSITDGALAICLGGLTSLRNLFLTEIMTLTTLPPEEVFQHLGNLR YLVIRSCWCLRSFGGLRSATSLSEIRLFSCPSLQLARGAEFMQMSLEKLCVYNCVLSA DFFCGDWPHLNNIGLCGCRSSASLYVGDLTSLKSFSLYHLPDLCVLEGLSYLQLHHVH LIDVPKLTTESISQFRVQRSLYISSSVMLNHMISAEGFKVPGFLSLESCKKPSVSFEE SANFTSVKCLRLCNCEMRSLPGNMKCLSSLTKLDIYDCPNITSLPDLPSSLQHICIWG CELLKKSCRAPDGESWPKIAHIRWKEFR</aaseq>|
DNA = <dnaseqindica>4282..8154#gggatctcccgccgttcggagacctaggggagcaagggggagactagagaaggttgcgctccatgaagtcatggtgcaccttaggtatcttgaaaggttttaccaagcgacctggttattgaagcgtggagaagaggctccagtgcggcaggagaaagcagatgagtagaggtacgatctcttcaggagaaagcagagcccagctgcccaggtggtggggagagattatcaaatttttcggtggtggtttggcacgggactctgccgcttcgctgccttcaaggctcaagctcagggtgactggcggcatctgacaggttcatttcctcttgcttatgtcttttagttgcaaatcaattaccattggttgtgggtttgaattattgggcgaaatgctcatgcgttggtcttgaaatttagctttttatctatctctatgttttacattataagtcgctttaatttttttctaattatttttttaagtttgatcaagttcatataaaaatgcaacaacattttcaactcaaaacaaatatattaatataatatattcaatgctatatgtaatgaaactaatttagtgttatgaatgttactatatttttttataaagttggttaaatctaaaaaggtttaattaaaaaaaatcaaaactacttataatacaagtaaaatagagagaattgcatagaagtggaagtgtgtcggtgtctgtactggttctactcacctagtcacctccctgccactgctattctgctaatagtgttagagaatacgcacgcacagataatggcaaggacgtcatttagaagagcccactagtgcgtgtcatgttgcttatgtgcaaattttatacactttcttgactgaccaaaggttgtatgcggtcattttttttttctctcggtgctagctttaatatacggaaagccgtttagagaagatggcatgacaataatggagcatatatatataaactagctcgtaccctgcggcgttgctgcgggtagatattgtaattgcaagtgtccagagaataacaaataaaattttccatggaacaatcatgatggccaaattgtataatatatggctgaaagttcacattatctaaaacaaatatttttcgctgcggtcggagtgtatattaagaaggagagagtttaagcaaactgtacaagagaacaatcagagccaaagctcacatacagaaccaggacactggttcgccacacacacatggttcaaacttcaaacattactgggagtctgatatacatttctttcttgtcttcctgcaaatttgtgttggcactagcttcatacagacctcaactctactgatccaagaacaacatgtagggctacatcctacatgtatacccaaatacccaatacaaacacaaaatagacgaagctaccctgcaatgcaatcagtaataagatcaatagttgataccgaagccaccagccagcatttcgggtaaacatagagcaacaatgaatataagcaagtctcttaagaattacgttctcattttgataaactggcaagcacgggtgaattttgcatcaacacccaacggaaatttgattagcattccttgaaatatgtcactgttgatgaaggtaaaaaagtaaacacaaagtgaagcccatagagcttcagtttagttcaggcatcaacatagtttaactcatgaacctccaaattaaataaattatttatttcattattatatatcataccttggtacatttgtcagcctcacggcagtagattttgcccatgttgcagtaagttacacacacaaacaccaaatatgctagtttttaaactattcttgtttcactagatttaattgcatttggttttttactatgctatttcggagttgtttaatttgatagtgggggccatagagtataatgaaaaacagcaaggtaatgagaaccagagcatagtccggctagaatattttaggagtccttttaatgaggtccagcttgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattctccaatttctccaaattgtaacatggtatcagacccttgttccaaaaaccctagccaccaaatccgatcatcaccggcgcaccggcggcggtgagccgccggcgagccatcatcatcgtcctccttcatccctcaacgtttttgtctccaagcagcaagcctgcagttcgtcgtcgtcaccgtgttcctgttcgccggtcgcagccgccgccagttgcggcgccgcgcccgttcccacttctccacccacgcagccacgccgtcgtggtgttcatccacgctgctcgtcgcggggctcgttcttgccgccgttcgagagccgccgctgtggcctcgtcaatctacatcaaggcatcaagcaacatcagtttttggcccatttcttgtcagttagcatcagcaaaccatcgtcctctctgatctgcagcagcaacaacaacaactcccgttcacctcctagtagatgcagcagctagcccattgttgtctcctaatttcagcagtagaatttccatgttccattgtttgtgttgcaacatgcattctgcagattgtcatcaagcttgtattccaccacatgtgttagtcagtttttgcgcttgctaatcgctgccatccactactcactcatcaatcatcaccatgctttgttattgttagctacttattataatccaattttacttctccaaattatcatgatggcttgtagtagtcactggttaggtcattagctgcttgcttgctactgtcaacatgttcactagcatcctttgtgtcttttgctagtaatgtgcatctctgcttgcagtccatctatcaatattgcctttttgctatatccatcaatccgtgttctttactaatcaaattcctgtgtttcctaccgagtccagcaaaatccattgcatatccttcattttgtcgaagctgtgccagtccatctcttcatggtcatttcatcatcaccagctccccgtcggagctggccttcattgtcacttcatatcttcatcatctccatgtcggagcttgtcatcatcatcatcaatcagtgaaggtccgaagaacagtttgtgaagggccttcatcaatatcaatgtctcatcatcaatcaaaggaccgaagaacagcttgtgaaggtccattgtcatcatcatcaacatctccgaatcaatgaagagccgaagaacgattcgtgaaggcttcaaggtaccgagggccgaagaacagtttgtgaaggctccgaggtccatttcattgaggagggggtcttttcttcttcatcatccaagaattgacacacttcgacgacatcatcaagactttggagataggctaatatttagattgtgttctcatgtttagtttacctttcaaatgcaatgttattacatacacattgcatttgagtgggggtgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattccccaatttctccaaattgtgacacagctcatctgaatattagagaaatataaatttgccactaacatttaacaatgtcccacttgtctactccctccgtcaaaaaaaaccaaacctaagacttagatgtgatataatctagtatctgtctagattcgttgtactagattatggcttaaatactagattaagttttttttgggacggagggagtatgtctaaccaaaataacacaattgatttgcatattgtgttttgcctcgcattgtgccgaagcaatcttatctgtggcaattggtagagttcttcctctcacaccttctctgcaatacaaacgcatctggctctggtaaccttgcactcctctcttagtatttctttccttcacgctttcttgtctctgcttaagttctctatcgtttggtaccatcatgcattaattcctttttcctctatttcccactccaaacatttccataaccactcacatacacaaaaataaaaaaccagacagagagaaaaaaacttgttttgggagttctgttgttatcttcctcacagaagaaaaatctctccatcaccatctgttcttatgccctgcttctattcattcacttgttcacagctgcagtagtgctgttccatggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaatgtgtttacaatgacaagagggtacaagaacattttgacgtcaggatatgggtctgcatctcacgcaaacttgatgttcatcgccatacacgtgagattatcgagtcagcaacaaatggagagtgccctcgtgtggacaatcttgatactctccagtgcagattgaaggacataatgcaaaaatcagagaaattcctgcttgtgttggatgacgtctggtttgatgaatccgtcaatgagagggaatgggaccagttgcttgatcctcttgtttctcagcaggaggggagcagagttttggtgacttctagacgagatgtacttccagctgctcttcactgtaaggatgttgttcatttggaaaacatggaagatgccgagttcttggctctattcaaataccatgctttctctggaacagaaatcagaaatccacagttgcatgcacggctagaagaggtcgcagagaagattgctaaaaggttaggacaatcgcctttagcagcaaggacagtaggctcccagctgagcaggaataaggatattgccatatggaaaagtgctctaaatattgaaaacttaagtgagcccatgaaagctctgttgtggagttataataaattagattcacgtctgcagagatgttttctgtactgcagcttatttccaaaaggtcacaagtataaaattgatgagatggttgacctttgggtggcagaggggctagttgattcacgcaaccagggggataagagaattgaagatattggcagggattacttcaatgagatggtgtcaggatctttctttcaaccagtttctgaaagatatatgggtacatggtacattatgcatgatctgcttcatgatttggcagagtcactgactaaagaagactgcttcagattagaagatgatggggtgaaagagataccagccactgttcgacatctatctatttgtgttgacagtatgaaattccataagcaaaagatctgcaagctacgttatttacgcactgttatctgcattgaccctctcatggatgacggagatgatatttttaatcagctattgaagaatctgaagaagctacgtgtactacatttgtcgttttacaacagtagcagcttgcctgaatgtattggtgagctgaagcaccttcgatatttgagtatcatcagcacattgatttctgaactgccaagatcattgtgtactcttttccacttggagctacttcatttgaatgacaaggtcaagaatttgccggacagactctgtaatttaagaaagttacgacgtcttgaagcatatgatgacagaaacagaatgtataaattgtatagagcagctctccctcaaattccttacataggcaagctatctttgctgcaagatattgatggattttgcgtgcaaaagcagaagggatatgagttgcgacaactgagggacatgaacaagctcggtggtaatttgcgtgtcgttaatcttgaaaatgtaactggaaaggatgaagcctcagagtcgaagttgcatcagaaaactcatcttagaggtttgcatctctcctggaatgatgtggatgacatggatgtttcacatttggagattctagaaggtttgaggccgccatctcaattggaggaccttacaatcgaaggttacaaatccaccatgtacccaagctggttacttgatggatcctattttgagaatctagagtcttttacgcttgctaattgctgtgtgataggaagcctaccacccaataccgagatctttaggcactgtatgacacttacccttgagaatgtcccgaatatgaagacactaccttttcttccagaaggtctcacaagcctatcaattgaggggtgcccactgcttgtgttcactactaataacgatgaactggaacaccatgattatagggagagcatcacgagggcaaacaacctggaaacacaacttgttttgatatgggaagcgaattccgattcagatattaggagcacattgtcgtccgaacactcatccatgaagaagttgacggaattgatggatactgatatgtcgggaaatcttcaaaccattgaaagcgctttagaaatagagagagatgaagccttggttaaagaagatatcatcaaagtatggctctgttgccacgaggagaggatgagattcatttattcaaggaaggctgggttgccgttggttctaccatctggactatgcgtactctctctttcctcctgtagtattacagatggagctttagctatttgccttggtgggcttacttcacttagaaatttgttcttaacagagattatgactttaactacgcttccaccggaagaggtcttccaacatttgggcaatcttagatacttggtcattcgttcctgctggtgtctcagatcattcgggggcttgcgatctgctacctctctttcagaaataagattattttcctgtccttctttacagttggcacgtggcgcagaatttatgcaaatgtcccttgagaagctatgtgtatacaactgtgtgctttcagctgacttcttctgcggtgactggccacatctgaataatattgggttatgtgggtgtagaagctctgcgtccttgtatgttggtgatcttacctccctcaaatcattctcactatatcatttgccagatttatgcgtgctggaaggtttgtcttacctacagcttcaccacgtgcatttgatagatgttccaaagcttactaccgagagcatctcacagttccgcgtacagcgttcgctctatattagcagttccgttatgctcaaccacatgatctccgctgaaggtttcaaagttccagggtttctctctcttgaaagctgcaagaagccatccgtttcattcgaagaatctgcaaatttcacatccgtcaagtgcctgaggttatgtaattgtgaaatgaggtcactaccaggaaatatgaagtgcctatccagtctaacgaaactcgatatctatgactgccccaacataacatctctaccagatctgccgtcctccctccagcatatatgtatatggggttgtgagcttttgaagaagagctgcagggcacctgatggagaaagctggccaaagattgcgcatatccgctggaaggaattcagatgaattgtgcttcagaatcactactacggagaagcgcaaaggtgaaggtcccatcaatgcattatcagaagtactctatgtaatatttatttcattcgctcatgatcttacgaacacatcattaacttcacgtttcctttgttttgcagacaccccaagatttgctctctcaggtaacttcgtctcttcgccacctgctacaaggtagagcgcctcatgctcatatagcagtttcagctctctcctgtagtcctgtggtctgtccggttgtgctgtccactctgttccctgcttcgtcgattgccacatttacgtttccctgctgccttctaccgacccacgcctcccttcatccggtctctgccactgacggcccatgatcgtgtccttttgctttcaccccttaaatggagtcttgttggtccaagaaaatctctgtttgtctgttgaacactcaactatctctcgtattttgtgtgatatcgctcgtatttgattgcgtgatgtgataatatattgaagtaattggattttgtacttcgcac</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185663.1 RefSeq:Os01g0782100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

Os01g0782100

2014-05-24T16:59:06Z

Liangdan2013: /* Expression */

Please input one-sentence summary here.
This rice blast resistance genes was found by two Japanese scholars Imbe and Matsumoto in 1985, they called this gene Pi-sh,this gene has the moderate resistance to the blast fungus strain Kyu77-07A in rice variety Shin-2.this gene has been characterized by using the method of classical genetic.And this gene is linking to pi-t.
==Annotated Information==
===Function===
The genes are accounting for the moderate resistance of the rice variety Shin 2 and some other varieties to the blast fungus strain Kyu 77-07A. Aichi Asahi (Pi-a) and carried out in the present study. Yashiro-mochi (Pi-ta), were found to be susceptible to the strain, while Ishikari Shirol*.e (Pi-i), Kanto 51 (Pi-k), Tsuyuake (Pi-k'!b), Fukunishiki (Pi-z) and Toride I (Pi-zt) were resistant, and Shin 2 (Pi-ks) and Pi N0.4 (Pi-ta2) moderately resistant. Therefore, Kyu 77-07A was classified as one of the strains belonging to race 102. Howver, the variety Reiho with the resistance gene Pi-ta2 was susceptible to this strain. Based on the knovn facts mentioned above, the objectives 0L the present study were' to determinA- whether Pi-ks is the gene responsible for the moderate resistance of Shin 2' to Kyu 77-07A along with the reason for the different reactions to Kyu 77-07A betweerL Pi N0.4 and Reiho which both have the same resistance gene Pi-ta2. For the gene analysis, the F2 or F3 plants of the crosses were inoculated with Kyu 77-07A and three other fungus strains using the spraying rnethod when the plants were at the four- to five-1eaf stage. Two varieties, Mineyutaka. and Saikai 155 belonging to the Shin 2 type varieties, were used as the representative parents of the varieties susceptible to Kyu 77-07A for the crosses.

===Expression===
To identify novel factors required for R gene-mediated resistance in rice, we used rice insertional mutant lines, induced by the endogenous retrotransposon Tos17, in a forward screen involving the rice blast fungus Magnaporthe oryzae. We inoculated 41,119 mutant lines with the fungus using a high throughput procedure, and identified 86 mutant lines with diminished resistance. A genome analysis revealed that 72 of the 86 lines contained mutations in a gene encoding a nucleotide binding site (NB) and leucine rich repeat (LRR) domain-containing (NLR) protein. A genetic complementation analysis and a pathogenesis assay demonstrated that this NLR gene encodes Pish, which confers resistance against races of M. oryzae containing avrPish. The other 14 lines have intact copies of the Pish gene, suggesting that they may contain mutations in the signaling components downstream of Pish. The genome analysis indicated that Pish and its neighboring three NLR genes are high similar to one another and are tandemly located. An in silico analysis of a Tos17 flanking sequence database revealed that this region is a "hot spot" for insertion. Intriguingly, the insertion sites are not distributed evenly among these four NLR genes, despite their similarity at the sequence and expression levels.
In this work we isolated the R gene Pish, and identified several other mutants involved in the signal transduction required for Pish-mediated resistance. These results indicate that our genetic approach is efficient and useful for unveiling novel aspects of defense signaling in rice. Furthermore, our data provide experimental evidence that R gene clusters have the potential to be highly preferred targets for transposable element insertions in plant genomes. Based on this finding, a possible mechanism underlying the high variability of R genes is discussed.
Primers Sequence direction target

===Evolution===
A study was conducted to investigate the relationship between phenotype of blast resistance and resistance gene analog polymorphism of rice, and search molecule hereditary basis of broad spectrum and durable resistance.
【Method】 Comparison of clustering analysis was investigated using spectrum of resistance to blast and polymorphism of resistance gene analog (RGA) in 25 varieties for blast resistance identification and 20 varieties lines).
【Result】 The resistance spectrum clustering analysis showed that the 45 varieties (lines) could be divided into group A and group B with the genetic similarity coefficient of 0.450. Group A and group B could be divided into subclassⅰ, subclass , ⅱ subclass , ⅲ subclass ⅳ, respectively, with 0.618 genetic similarity coefficient. The RGA-PCR clustering analysis showed that proposed the 45 varieties (lines) could be divided into group Ⅰ and group Ⅱ which clearly inclined the Indica-japonica differentiation with 0.620 genetic similarity coefficient. Group Ⅰ could be divided into six subclasses and group Ⅱ could be divided into seven subclasses with 0.783 genetic similarity coefficient. The resistance spectrum clustering analysis showed that some varieties with similar resistance spectrum could finely fall into the same group, while the RGA-PCR clustering analysis showed that some varieties with the similar genetic background could fall into the same group. For some varieties with low resistance frequency or high resistance frequency, there was a better corresponding relationship between the resistance spectrum clustering and the RGA-PCR clustering. General comparison of clustering analysis showed that here was no parallelism relationship between group and group in two different types of the clustering.
【Conclusion】 It could more accurately reflect their enetic background to test resistance to single strain and analysis on RGA polymorphism for resistance parents, and avoid applying the same source of resistance gain and again, and enrich rice resistance germplasm, and breed durable resistance varieties.

You can also add sub-section(s) at will.

==Labs working on this gene==
1.Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Ibaraki, 305-8602，Japan.
2.International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines;
3.Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, Japan;
4.Indonesian Center for Rice Research (ICRR), JL. Raya Muara No. 25A Ciapus Bogor, Subang, West Java, Indonesia;
5.Agricultural Genetics Institute,Conhue, Tuliem, Hanoi, Vietnam;
6.Faculty of Agricultural Sciences, University of Burundi, BP 2940 Bujumbura, Burundi;

==References==
1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta
Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.)
Plant Breeding, 2010, 129(6): 670-675
2. Akira Takahashi;Nagao Hayashi;Akio Miyao;Hirohiko Hirochika
Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging
BMC Plant Biology, 2010, 10: 175
3. Tokio IMBE;Shohei MATSUMOTO
Inheritance of Resrstance of Rice Vanetles to the Blast Fungus Strains Virulent to the Variety "Reiho"
Japanese Journal of Breeding, 1985, 35(0): 332-339.
4.鄂志国, 张丽靖, 焦桂爱, 等. 稻瘟病抗性基因的鉴定及利用进展[J]. 中国水稻科学, 2008, 22(5): 533-540.
5.杨勤忠, 林菲, 冯淑杰, 等. 水稻稻瘟病抗性基因的分子定位及克隆研究进展[J]. 中国农业科学, 2009, 42(5): 1601-1615.
6.任鄄胜, 肖培村, 陈勇, 等. 水稻品种稻瘟病抗性和抗病基因同源序列多态性分析[J]. 中国农业科学, 2009, 42(1): 1-9.
==Structured Information==
{{JaponicaGene|
GeneName = Os01g0782100|
Description = Similar to NBS-LRR protein (Fragment)|
Version = NM_001185663.1 GI:297720460 GeneID:9271927|
Length = 8696 bp|
Definition = Oryza sativa Japonica Group Os01g0782100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:34892692..34901387|
CDS = 34896973..34900845|
GCID = <gbrowseImage1>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaatgtgtttacaatgacaagagggtacaagaacattttgacgtcaggatatgggtctgcatctcacgcaaacttgatgttcatcgccatacacgtgagattatcgagtcagcaacaaatggagagtgccctcgtgtggacaatcttgatactctccagtgcagattgaaggacataatgcaaaaatcagagaaattcctgcttgtgttggatgacgtctggtttgatgaatccgtcaatgagagggaatgggaccagttgcttgatcctcttgtttctcagcaggaggggagcagagttttggtgacttctagacgagatgtacttccagctgctcttcactgtaaggatgttgttcatttggaaaacatggaagatgccgagttcttggctctattcaaataccatgctttctctggaacagaaatcagaaatccacagttgcatgcacggctagaagaggtcgcagagaagattgctaaaaggttaggacaatcgcctttagcagcaaggacagtaggctcccagctgagcaggaataaggatattgccatatggaaaagtgctctaaatattgaaaacttaagtgagcccatgaaagctctgttgtggagttataataaattagattcacgtctgcagagatgttttctgtactgcagcttatttccaaaaggtcacaagtataaaattgatgagatggttgacctttgggtggcagaggggctagttgattcacgcaaccagggggataagagaattgaagatattggcagggattacttcaatgagatggtgtcaggatctttctttcaaccagtttctgaaagatatatgggtacatggtacattatgcatgatctgcttcatgatttggcagagtcactgactaaagaagactgcttcagattagaagatgatggggtgaaagagataccagccactgttcgacatctatctatttgtgttgacagtatgaaattccataagcaaaagatctgcaagctacgttatttacgcactgttatctgcattgaccctctcatggatgacggagatgatatttttaatcagctattgaagaatctgaagaagctacgtgtactacatttgtcgttttacaacagtagcagcttgcctgaatgtattggtgagctgaagcaccttcgatatttgagtatcatcagcacattgatttctgaactgccaagatcattgtgtactcttttccacttggagctacttcatttgaatgacaaggtcaagaatttgccggacagactctgtaatttaagaaagttacgacgtcttgaagcatatgatgacagaaacagaatgtataaattgtatagagcagctctccctcaaattccttacataggcaagctatctttgctgcaagatattgatggattttgcgtgcaaaagcagaagggatatgagttgcgacaactgagggacatgaacaagctcggtggtaatttgcgtgtcgttaatcttgaaaatgtaactggaaaggatgaagcctcagagtcgaagttgcatcagaaaactcatcttagaggtttgcatctctcctggaatgatgtggatgacatggatgtttcacatttggagattctagaaggtttgaggccgccatctcaattggaggaccttacaatcgaaggttacaaatccaccatgtacccaagctggttacttgatggatcctattttgagaatctagagtcttttacgcttgctaattgctgtgtgataggaagcctaccacccaataccgagatctttaggcactgtatgacacttacccttgagaatgtcccgaatatgaagacactaccttttcttccagaaggtctcacaagcctatcaattgaggggtgcccactgcttgtgttcactactaataacgatgaactggaacaccatgattatagggagagcatcacgagggcaaacaacctggaaacacaacttgttttgatatgggaagcgaattccgattcagatattaggagcacattgtcgtccgaacactcatccatgaagaagttgacggaattgatggatactgatatgtcgggaaatcttcaaaccattgaaagcgctttagaaatagagagagatgaagccttggttaaagaagatatcatcaaagtatggctctgttgccacgaggagaggatgagattcatttattcaaggaaggctgggttgccgttggttctaccatctggactatgcgtactctctctttcctcctgtagtattacagatggagctttagctatttgccttggtgggcttacttcacttagaaatttgttcttaacagagattatgactttaactacgcttccaccggaagaggtcttccaacatttgggcaatcttagatacttggtcattcgttcctgctggtgtctcagatcattcgggggcttgcgatctgctacctctctttcagaaataagattattttcctgtccttctttacagttggcacgtggcgcagaatttatgcaaatgtcccttgagaagctatgtgtatacaactgtgtgctttcagctgacttcttctgcggtgactggccacatctgaataatattgggttatgtgggtgtagaagctctgcgtccttgtatgttggtgatcttacctccctcaaatcattctcactatatcatttgccagatttatgcgtgctggaaggtttgtcttacctacagcttcaccacgtgcatttgatagatgttccaaagcttactaccgagagcatctcacagttccgcgtacagcgttcgctctatattagcagttccgttatgctcaaccacatgatctccgctgaaggtttcaaagttccagggtttctctctcttgaaagctgcaagaagccatccgtttcattcgaagaatctgcaaatttcacatccgtcaagtgcctgaggttatgtaattgtgaaatgaggtcactaccaggaaatatgaagtgcctatccagtctaacgaaactcgatatctatgactgccccaacataacatctctaccagatctgccgtcctccctccagcatatatgtatatggggttgtgagcttttgaagaagagctgcagggcacctgatggagaaagctggccaaagattgcgcatatccgctggaaggaattcagatga</cdnaseq>|
AA = <aaseq>MAEVVLAGLRLAATPICVKLLCNASTCLGVDMTRELHELETIII PQFELVIEAAEKGNHRAKLDRWLRELKQAFYNAEDLLDEHEYNILKCKAKHKDSLVKD STQVHDSSISNILKQPMRAVSSRMSNLRPENRKILCQLNELKTMLEKAKEFRELIHLP AGNSLEGPSVPTIVVPVVTSLLPPRVFGRNMDRDRIIHLLTKPMATVSSSVGYSGLAI VAHGGAGKSTLAQCVYNDKRVQEHFDVRIWVCISRKLDVHRHTREIIESATNGECPRV DNLDTLQCRLKDIMQKSEKFLLVLDDVWFDESVNEREWDQLLDPLVSQQEGSRVLVTS RRDVLPAALHCKDVVHLENMEDAEFLALFKYHAFSGTEIRNPQLHARLEEVAEKIAKR LGQSPLAARTVGSQLSRNKDIAIWKSALNIENLSEPMKALLWSYNKLDSRLQRCFLYC SLFPKGHKYKIDEMVDLWVAEGLVDSRNQGDKRIEDIGRDYFNEMVSGSFFQPVSERY MGTWYIMHDLLHDLAESLTKEDCFRLEDDGVKEIPATVRHLSICVDSMKFHKQKICKL RYLRTVICIDPLMDDGDDIFNQLLKNLKKLRVLHLSFYNSSSLPECIGELKHLRYLSI ISTLISELPRSLCTLFHLELLHLNDKVKNLPDRLCNLRKLRRLEAYDDRNRMYKLYRA ALPQIPYIGKLSLLQDIDGFCVQKQKGYELRQLRDMNKLGGNLRVVNLENVTGKDEAS ESKLHQKTHLRGLHLSWNDVDDMDVSHLEILEGLRPPSQLEDLTIEGYKSTMYPSWLL DGSYFENLESFTLANCCVIGSLPPNTEIFRHCMTLTLENVPNMKTLPFLPEGLTSLSI EGCPLLVFTTNNDELEHHDYRESITRANNLETQLVLIWEANSDSDIRSTLSSEHSSMK KLTELMDTDMSGNLQTIESALEIERDEALVKEDIIKVWLCCHEERMRFIYSRKAGLPL VLPSGLCVLSLSSCSITDGALAICLGGLTSLRNLFLTEIMTLTTLPPEEVFQHLGNLR YLVIRSCWCLRSFGGLRSATSLSEIRLFSCPSLQLARGAEFMQMSLEKLCVYNCVLSA DFFCGDWPHLNNIGLCGCRSSASLYVGDLTSLKSFSLYHLPDLCVLEGLSYLQLHHVH LIDVPKLTTESISQFRVQRSLYISSSVMLNHMISAEGFKVPGFLSLESCKKPSVSFEE SANFTSVKCLRLCNCEMRSLPGNMKCLSSLTKLDIYDCPNITSLPDLPSSLQHICIWG CELLKKSCRAPDGESWPKIAHIRWKEFR</aaseq>|
DNA = <dnaseqindica>4282..8154#gggatctcccgccgttcggagacctaggggagcaagggggagactagagaaggttgcgctccatgaagtcatggtgcaccttaggtatcttgaaaggttttaccaagcgacctggttattgaagcgtggagaagaggctccagtgcggcaggagaaagcagatgagtagaggtacgatctcttcaggagaaagcagagcccagctgcccaggtggtggggagagattatcaaatttttcggtggtggtttggcacgggactctgccgcttcgctgccttcaaggctcaagctcagggtgactggcggcatctgacaggttcatttcctcttgcttatgtcttttagttgcaaatcaattaccattggttgtgggtttgaattattgggcgaaatgctcatgcgttggtcttgaaatttagctttttatctatctctatgttttacattataagtcgctttaatttttttctaattatttttttaagtttgatcaagttcatataaaaatgcaacaacattttcaactcaaaacaaatatattaatataatatattcaatgctatatgtaatgaaactaatttagtgttatgaatgttactatatttttttataaagttggttaaatctaaaaaggtttaattaaaaaaaatcaaaactacttataatacaagtaaaatagagagaattgcatagaagtggaagtgtgtcggtgtctgtactggttctactcacctagtcacctccctgccactgctattctgctaatagtgttagagaatacgcacgcacagataatggcaaggacgtcatttagaagagcccactagtgcgtgtcatgttgcttatgtgcaaattttatacactttcttgactgaccaaaggttgtatgcggtcattttttttttctctcggtgctagctttaatatacggaaagccgtttagagaagatggcatgacaataatggagcatatatatataaactagctcgtaccctgcggcgttgctgcgggtagatattgtaattgcaagtgtccagagaataacaaataaaattttccatggaacaatcatgatggccaaattgtataatatatggctgaaagttcacattatctaaaacaaatatttttcgctgcggtcggagtgtatattaagaaggagagagtttaagcaaactgtacaagagaacaatcagagccaaagctcacatacagaaccaggacactggttcgccacacacacatggttcaaacttcaaacattactgggagtctgatatacatttctttcttgtcttcctgcaaatttgtgttggcactagcttcatacagacctcaactctactgatccaagaacaacatgtagggctacatcctacatgtatacccaaatacccaatacaaacacaaaatagacgaagctaccctgcaatgcaatcagtaataagatcaatagttgataccgaagccaccagccagcatttcgggtaaacatagagcaacaatgaatataagcaagtctcttaagaattacgttctcattttgataaactggcaagcacgggtgaattttgcatcaacacccaacggaaatttgattagcattccttgaaatatgtcactgttgatgaaggtaaaaaagtaaacacaaagtgaagcccatagagcttcagtttagttcaggcatcaacatagtttaactcatgaacctccaaattaaataaattatttatttcattattatatatcataccttggtacatttgtcagcctcacggcagtagattttgcccatgttgcagtaagttacacacacaaacaccaaatatgctagtttttaaactattcttgtttcactagatttaattgcatttggttttttactatgctatttcggagttgtttaatttgatagtgggggccatagagtataatgaaaaacagcaaggtaatgagaaccagagcatagtccggctagaatattttaggagtccttttaatgaggtccagcttgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattctccaatttctccaaattgtaacatggtatcagacccttgttccaaaaaccctagccaccaaatccgatcatcaccggcgcaccggcggcggtgagccgccggcgagccatcatcatcgtcctccttcatccctcaacgtttttgtctccaagcagcaagcctgcagttcgtcgtcgtcaccgtgttcctgttcgccggtcgcagccgccgccagttgcggcgccgcgcccgttcccacttctccacccacgcagccacgccgtcgtggtgttcatccacgctgctcgtcgcggggctcgttcttgccgccgttcgagagccgccgctgtggcctcgtcaatctacatcaaggcatcaagcaacatcagtttttggcccatttcttgtcagttagcatcagcaaaccatcgtcctctctgatctgcagcagcaacaacaacaactcccgttcacctcctagtagatgcagcagctagcccattgttgtctcctaatttcagcagtagaatttccatgttccattgtttgtgttgcaacatgcattctgcagattgtcatcaagcttgtattccaccacatgtgttagtcagtttttgcgcttgctaatcgctgccatccactactcactcatcaatcatcaccatgctttgttattgttagctacttattataatccaattttacttctccaaattatcatgatggcttgtagtagtcactggttaggtcattagctgcttgcttgctactgtcaacatgttcactagcatcctttgtgtcttttgctagtaatgtgcatctctgcttgcagtccatctatcaatattgcctttttgctatatccatcaatccgtgttctttactaatcaaattcctgtgtttcctaccgagtccagcaaaatccattgcatatccttcattttgtcgaagctgtgccagtccatctcttcatggtcatttcatcatcaccagctccccgtcggagctggccttcattgtcacttcatatcttcatcatctccatgtcggagcttgtcatcatcatcatcaatcagtgaaggtccgaagaacagtttgtgaagggccttcatcaatatcaatgtctcatcatcaatcaaaggaccgaagaacagcttgtgaaggtccattgtcatcatcatcaacatctccgaatcaatgaagagccgaagaacgattcgtgaaggcttcaaggtaccgagggccgaagaacagtttgtgaaggctccgaggtccatttcattgaggagggggtcttttcttcttcatcatccaagaattgacacacttcgacgacatcatcaagactttggagataggctaatatttagattgtgttctcatgtttagtttacctttcaaatgcaatgttattacatacacattgcatttgagtgggggtgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattccccaatttctccaaattgtgacacagctcatctgaatattagagaaatataaatttgccactaacatttaacaatgtcccacttgtctactccctccgtcaaaaaaaaccaaacctaagacttagatgtgatataatctagtatctgtctagattcgttgtactagattatggcttaaatactagattaagttttttttgggacggagggagtatgtctaaccaaaataacacaattgatttgcatattgtgttttgcctcgcattgtgccgaagcaatcttatctgtggcaattggtagagttcttcctctcacaccttctctgcaatacaaacgcatctggctctggtaaccttgcactcctctcttagtatttctttccttcacgctttcttgtctctgcttaagttctctatcgtttggtaccatcatgcattaattcctttttcctctatttcccactccaaacatttccataaccactcacatacacaaaaataaaaaaccagacagagagaaaaaaacttgttttgggagttctgttgttatcttcctcacagaagaaaaatctctccatcaccatctgttcttatgccctgcttctattcattcacttgttcacagctgcagtagtgctgttccatggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaat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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185663.1 RefSeq:Os01g0782100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

Os01g0782100

2014-05-24T16:52:56Z

Liangdan2013: /* Expression */

Please input one-sentence summary here.
This rice blast resistance genes was found by two Japanese scholars Imbe and Matsumoto in 1985, they called this gene Pi-sh,this gene has the moderate resistance to the blast fungus strain Kyu77-07A in rice variety Shin-2.this gene has been characterized by using the method of classical genetic.And this gene is linking to pi-t.
==Annotated Information==
===Function===
The genes are accounting for the moderate resistance of the rice variety Shin 2 and some other varieties to the blast fungus strain Kyu 77-07A. Aichi Asahi (Pi-a) and carried out in the present study. Yashiro-mochi (Pi-ta), were found to be susceptible to the strain, while Ishikari Shirol*.e (Pi-i), Kanto 51 (Pi-k), Tsuyuake (Pi-k'!b), Fukunishiki (Pi-z) and Toride I (Pi-zt) were resistant, and Shin 2 (Pi-ks) and Pi N0.4 (Pi-ta2) moderately resistant. Therefore, Kyu 77-07A was classified as one of the strains belonging to race 102. Howver, the variety Reiho with the resistance gene Pi-ta2 was susceptible to this strain. Based on the knovn facts mentioned above, the objectives 0L the present study were' to determinA- whether Pi-ks is the gene responsible for the moderate resistance of Shin 2' to Kyu 77-07A along with the reason for the different reactions to Kyu 77-07A betweerL Pi N0.4 and Reiho which both have the same resistance gene Pi-ta2. For the gene analysis, the F2 or F3 plants of the crosses were inoculated with Kyu 77-07A and three other fungus strains using the spraying rnethod when the plants were at the four- to five-1eaf stage. Two varieties, Mineyutaka. and Saikai 155 belonging to the Shin 2 type varieties, were used as the representative parents of the varieties susceptible to Kyu 77-07A for the crosses.

===Expression===
To identify novel factors required for R gene-mediated resistance in rice, we used rice insertional mutant lines, induced by the endogenous retrotransposon Tos17, in a forward screen involving the rice blast fungus Magnaporthe oryzae. We inoculated 41,119 mutant lines with the fungus using a high throughput procedure, and identified 86 mutant lines with diminished resistance. A genome analysis revealed that 72 of the 86 lines contained mutations in a gene encoding a nucleotide binding site (NB) and leucine rich repeat (LRR) domain-containing (NLR) protein. A genetic complementation analysis and a pathogenesis assay demonstrated that this NLR gene encodes Pish, which confers resistance against races of M. oryzae containing avrPish. The other 14 lines have intact copies of the Pish gene, suggesting that they may contain mutations in the signaling components downstream of Pish. The genome analysis indicated that Pish and its neighboring three NLR genes are high similar to one another and are tandemly located. An in silico analysis of a Tos17 flanking sequence database revealed that this region is a "hot spot" for insertion. Intriguingly, the insertion sites are not distributed evenly among these four NLR genes, despite their similarity at the sequence and expression levels.
In this work we isolated the R gene Pish, and identified several other mutants involved in the signal transduction required for Pish-mediated resistance. These results indicate that our genetic approach is efficient and useful for unveiling novel aspects of defense signaling in rice. Furthermore, our data provide experimental evidence that R gene clusters have the potential to be highly preferred targets for transposable element insertions in plant genomes. Based on this finding, a possible mechanism underlying the high variability of R genes is discussed.
Primers Sequence direction target
[[新加卷(F):sequence.jpg]]

===Evolution===
A study was conducted to investigate the relationship between phenotype of blast resistance and resistance gene analog polymorphism of rice, and search molecule hereditary basis of broad spectrum and durable resistance.
【Method】 Comparison of clustering analysis was investigated using spectrum of resistance to blast and polymorphism of resistance gene analog (RGA) in 25 varieties for blast resistance identification and 20 varieties lines).
【Result】 The resistance spectrum clustering analysis showed that the 45 varieties (lines) could be divided into group A and group B with the genetic similarity coefficient of 0.450. Group A and group B could be divided into subclassⅰ, subclass , ⅱ subclass , ⅲ subclass ⅳ, respectively, with 0.618 genetic similarity coefficient. The RGA-PCR clustering analysis showed that proposed the 45 varieties (lines) could be divided into group Ⅰ and group Ⅱ which clearly inclined the Indica-japonica differentiation with 0.620 genetic similarity coefficient. Group Ⅰ could be divided into six subclasses and group Ⅱ could be divided into seven subclasses with 0.783 genetic similarity coefficient. The resistance spectrum clustering analysis showed that some varieties with similar resistance spectrum could finely fall into the same group, while the RGA-PCR clustering analysis showed that some varieties with the similar genetic background could fall into the same group. For some varieties with low resistance frequency or high resistance frequency, there was a better corresponding relationship between the resistance spectrum clustering and the RGA-PCR clustering. General comparison of clustering analysis showed that here was no parallelism relationship between group and group in two different types of the clustering.
【Conclusion】 It could more accurately reflect their enetic background to test resistance to single strain and analysis on RGA polymorphism for resistance parents, and avoid applying the same source of resistance gain and again, and enrich rice resistance germplasm, and breed durable resistance varieties.

You can also add sub-section(s) at will.

==Labs working on this gene==
1.Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Ibaraki, 305-8602，Japan.
2.International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines;
3.Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, Japan;
4.Indonesian Center for Rice Research (ICRR), JL. Raya Muara No. 25A Ciapus Bogor, Subang, West Java, Indonesia;
5.Agricultural Genetics Institute,Conhue, Tuliem, Hanoi, Vietnam;
6.Faculty of Agricultural Sciences, University of Burundi, BP 2940 Bujumbura, Burundi;

==References==
1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta
Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.)
Plant Breeding, 2010, 129(6): 670-675
2. Akira Takahashi;Nagao Hayashi;Akio Miyao;Hirohiko Hirochika
Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging
BMC Plant Biology, 2010, 10: 175
3. Tokio IMBE;Shohei MATSUMOTO
Inheritance of Resrstance of Rice Vanetles to the Blast Fungus Strains Virulent to the Variety "Reiho"
Japanese Journal of Breeding, 1985, 35(0): 332-339.
4.鄂志国, 张丽靖, 焦桂爱, 等. 稻瘟病抗性基因的鉴定及利用进展[J]. 中国水稻科学, 2008, 22(5): 533-540.
5.杨勤忠, 林菲, 冯淑杰, 等. 水稻稻瘟病抗性基因的分子定位及克隆研究进展[J]. 中国农业科学, 2009, 42(5): 1601-1615.
6.任鄄胜, 肖培村, 陈勇, 等. 水稻品种稻瘟病抗性和抗病基因同源序列多态性分析[J]. 中国农业科学, 2009, 42(1): 1-9.
==Structured Information==
{{JaponicaGene|
GeneName = Os01g0782100|
Description = Similar to NBS-LRR protein (Fragment)|
Version = NM_001185663.1 GI:297720460 GeneID:9271927|
Length = 8696 bp|
Definition = Oryza sativa Japonica Group Os01g0782100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:34892692..34901387|
CDS = 34896973..34900845|
GCID = <gbrowseImage1>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaatgtgtttacaatgacaagagggtacaagaacattttgacgtcaggatatgggtctgcatctcacgcaaacttgatgttcatcgccatacacgtgagattatcgagtcagcaacaaatggagagtgccctcgtgtggacaatcttgatactctccagtgcagattgaaggacataatgcaaaaatcagagaaattcctgcttgtgttggatgacgtctggtttgatgaatccgtcaatgagagggaatgggaccagttgcttgatcctcttgtttctcagcaggaggggagcagagttttggtgacttctagacgagatgtacttccagctgctcttcactgtaaggatgttgttcatttggaaaacatggaagatgccgagttcttggctctattcaaataccatgctttctctggaacagaaatcagaaatccacagttgcatgcacggctagaagaggtcgcagagaagattgctaaaaggttaggacaatcgcctttagcagcaaggacagtaggctcccagctgagcaggaataaggatattgccatatggaaaagtgctctaaatattgaaaacttaagtgagcccatgaaagctctgttgtggagttataataaattagattcacgtctgcagagatgttttctgtactgcagcttatttccaaaaggtcacaagtataaaattgatgagatggttgacctttgggtggcagaggggctagttgattcacgcaaccagggggataagagaattgaagatattggcagggattacttcaatgagatggtgtcaggatctttctttcaaccagtttctgaaagatatatgggtacatggtacattatgcatgatctgcttcatgatttggcagagtcactgactaaagaagactgcttcagattagaagatgatggggtgaaagagataccagccactgttcgacatctatctatttgtgttgacagtatgaaattccataagcaaaagatctgcaagctacgttatttacgcactgttatctgcattgaccctctcatggatgacggagatgatatttttaatcagctattgaagaatctgaagaagctacgtgtactacatttgtcgttttacaacagtagcagcttgcctgaatgtattggtgagctgaagcaccttcgatatttgagtatcatcagcacattgatttctgaactgccaagatcattgtgtactcttttccacttggagctacttcatttgaatgacaaggtcaagaatttgccggacagactctgtaatttaagaaagttacgacgtcttgaagcatatgatgacagaaacagaatgtataaattgtatagagcagctctccctcaaattccttacataggcaagctatctttgctgcaagatattgatggattttgcgtgcaaaagcagaagggatatgagttgcgacaactgagggacatgaacaagctcggtggtaatttgcgtgtcgttaatcttgaaaatgtaactggaaaggatgaagcctcagagtcgaagttgcatcagaaaactcatcttagaggtttgcatctctcctggaatgatgtggatgacatggatgtttcacatttggagattctagaaggtttgaggccgccatctcaattggaggaccttacaatcgaaggttacaaatccaccatgtacccaagctggttacttgatggatcctattttgagaatctagagtcttttacgcttgctaattgctgtgtgataggaagcctaccacccaataccgagatctttaggcactgtatgacacttacccttgagaatgtcccgaatatgaagacactaccttttcttccagaaggtctcacaagcctatcaattgaggggtgcccactgcttgtgttcactactaataacgatgaactggaacaccatgattatagggagagcatcacgagggcaaacaacctggaaacacaacttgttttgatatgggaagcgaattccgattcagatattaggagcacattgtcgtccgaacactcatccatgaagaagttgacggaattgatggatactgatatgtcgggaaatcttcaaaccattgaaagcgctttagaaatagagagagatgaagccttggttaaagaagatatcatcaaagtatggctctgttgccacgaggagaggatgagattcatttattcaaggaaggctgggttgccgttggttctaccatctggactatgcgtactctctctttcctcctgtagtattacagatggagctttagctatttgccttggtgggcttacttcacttagaaatttgttcttaacagagattatgactttaactacgcttccaccggaagaggtcttccaacatttgggcaatcttagatacttggtcattcgttcctgctggtgtctcagatcattcgggggcttgcgatctgctacctctctttcagaaataagattattttcctgtccttctttacagttggcacgtggcgcagaatttatgcaaatgtcccttgagaagctatgtgtatacaactgtgtgctttcagctgacttcttctgcggtgactggccacatctgaataatattgggttatgtgggtgtagaagctctgcgtccttgtatgttggtgatcttacctccctcaaatcattctcactatatcatttgccagatttatgcgtgctggaaggtttgtcttacctacagcttcaccacgtgcatttgatagatgttccaaagcttactaccgagagcatctcacagttccgcgtacagcgttcgctctatattagcagttccgttatgctcaaccacatgatctccgctgaaggtttcaaagttccagggtttctctctcttgaaagctgcaagaagccatccgtttcattcgaagaatctgcaaatttcacatccgtcaagtgcctgaggttatgtaattgtgaaatgaggtcactaccaggaaatatgaagtgcctatccagtctaacgaaactcgatatctatgactgccccaacataacatctctaccagatctgccgtcctccctccagcatatatgtatatggggttgtgagcttttgaagaagagctgcagggcacctgatggagaaagctggccaaagattgcgcatatccgctggaaggaattcagatga</cdnaseq>|
AA = <aaseq>MAEVVLAGLRLAATPICVKLLCNASTCLGVDMTRELHELETIII PQFELVIEAAEKGNHRAKLDRWLRELKQAFYNAEDLLDEHEYNILKCKAKHKDSLVKD STQVHDSSISNILKQPMRAVSSRMSNLRPENRKILCQLNELKTMLEKAKEFRELIHLP AGNSLEGPSVPTIVVPVVTSLLPPRVFGRNMDRDRIIHLLTKPMATVSSSVGYSGLAI VAHGGAGKSTLAQCVYNDKRVQEHFDVRIWVCISRKLDVHRHTREIIESATNGECPRV DNLDTLQCRLKDIMQKSEKFLLVLDDVWFDESVNEREWDQLLDPLVSQQEGSRVLVTS RRDVLPAALHCKDVVHLENMEDAEFLALFKYHAFSGTEIRNPQLHARLEEVAEKIAKR LGQSPLAARTVGSQLSRNKDIAIWKSALNIENLSEPMKALLWSYNKLDSRLQRCFLYC SLFPKGHKYKIDEMVDLWVAEGLVDSRNQGDKRIEDIGRDYFNEMVSGSFFQPVSERY MGTWYIMHDLLHDLAESLTKEDCFRLEDDGVKEIPATVRHLSICVDSMKFHKQKICKL RYLRTVICIDPLMDDGDDIFNQLLKNLKKLRVLHLSFYNSSSLPECIGELKHLRYLSI ISTLISELPRSLCTLFHLELLHLNDKVKNLPDRLCNLRKLRRLEAYDDRNRMYKLYRA ALPQIPYIGKLSLLQDIDGFCVQKQKGYELRQLRDMNKLGGNLRVVNLENVTGKDEAS ESKLHQKTHLRGLHLSWNDVDDMDVSHLEILEGLRPPSQLEDLTIEGYKSTMYPSWLL DGSYFENLESFTLANCCVIGSLPPNTEIFRHCMTLTLENVPNMKTLPFLPEGLTSLSI EGCPLLVFTTNNDELEHHDYRESITRANNLETQLVLIWEANSDSDIRSTLSSEHSSMK KLTELMDTDMSGNLQTIESALEIERDEALVKEDIIKVWLCCHEERMRFIYSRKAGLPL VLPSGLCVLSLSSCSITDGALAICLGGLTSLRNLFLTEIMTLTTLPPEEVFQHLGNLR YLVIRSCWCLRSFGGLRSATSLSEIRLFSCPSLQLARGAEFMQMSLEKLCVYNCVLSA DFFCGDWPHLNNIGLCGCRSSASLYVGDLTSLKSFSLYHLPDLCVLEGLSYLQLHHVH LIDVPKLTTESISQFRVQRSLYISSSVMLNHMISAEGFKVPGFLSLESCKKPSVSFEE SANFTSVKCLRLCNCEMRSLPGNMKCLSSLTKLDIYDCPNITSLPDLPSSLQHICIWG CELLKKSCRAPDGESWPKIAHIRWKEFR</aaseq>|
DNA = <dnaseqindica>4282..8154#gggatctcccgccgttcggagacctaggggagcaagggggagactagagaaggttgcgctccatgaagtcatggtgcaccttaggtatcttgaaaggttttaccaagcgacctggttattgaagcgtggagaagaggctccagtgcggcaggagaaagcagatgagtagaggtacgatctcttcaggagaaagcagagcccagctgcccaggtggtggggagagattatcaaatttttcggtggtggtttggcacgggactctgccgcttcgctgccttcaaggctcaagctcagggtgactggcggcatctgacaggttcatttcctcttgcttatgtcttttagttgcaaatcaattaccattggttgtgggtttgaattattgggcgaaatgctcatgcgttggtcttgaaatttagctttttatctatctctatgttttacattataagtcgctttaatttttttctaattatttttttaagtttgatcaagttcatataaaaatgcaacaacattttcaactcaaaacaaatatattaatataatatattcaatgctatatgtaatgaaactaatttagtgttatgaatgttactatatttttttataaagttggttaaatctaaaaaggtttaattaaaaaaaatcaaaactacttataatacaagtaaaatagagagaattgcatagaagtggaagtgtgtcggtgtctgtactggttctactcacctagtcacctccctgccactgctattctgctaatagtgttagagaatacgcacgcacagataatggcaaggacgtcatttagaagagcccactagtgcgtgtcatgttgcttatgtgcaaattttatacactttcttgactgaccaaaggttgtatgcggtcattttttttttctctcggtgctagctttaatatacggaaagccgtttagagaagatggcatgacaataatggagcatatatatataaactagctcgtaccctgcggcgttgctgcgggtagatattgtaattgcaagtgtccagagaataacaaataaaattttccatggaacaatcatgatggccaaattgtataatatatggctgaaagttcacattatctaaaacaaatatttttcgctgcggtcggagtgtatattaagaaggagagagtttaagcaaactgtacaagagaacaatcagagccaaagctcacatacagaaccaggacactggttcgccacacacacatggttcaaacttcaaacattactgggagtctgatatacatttctttcttgtcttcctgcaaatttgtgttggcactagcttcatacagacctcaactctactgatccaagaacaacatgtagggctacatcctacatgtatacccaaatacccaatacaaacacaaaatagacgaagctaccctgcaatgcaatcagtaataagatcaatagttgataccgaagccaccagccagcatttcgggtaaacatagagcaacaatgaatataagcaagtctcttaagaattacgttctcattttgataaactggcaagcacgggtgaattttgcatcaacacccaacggaaatttgattagcattccttgaaatatgtcactgttgatgaaggtaaaaaagtaaacacaaagtgaagcccatagagcttcagtttagttcaggcatcaacatagtttaactcatgaacctccaaattaaataaattatttatttcattattatatatcataccttggtacatttgtcagcctcacggcagtagattttgcccatgttgcagtaagttacacacacaaacaccaaatatgctagtttttaaactattcttgtttcactagatttaattgcatttggttttttactatgctatttcggagttgtttaatttgatagtgggggccatagagtataatgaaaaacagcaaggtaatgagaaccagagcatagtccggctagaatattttaggagtccttttaatgaggtccagcttgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattctccaatttctccaaattgtaacatggtatcagacccttgttccaaaaaccctagccaccaaatccgatcatcaccggcgcaccggcggcggtgagccgccggcgagccatcatcatcgtcctccttcatccctcaacgtttttgtctccaagcagcaagcctgcagttcgtcgtcgtcaccgtgttcctgttcgccggtcgcagccgccgccagttgcggcgccgcgcccgttcccacttctccacccacgcagccacgccgtcgtggtgttcatccacgctgctcgtcgcggggctcgttcttgccgccgttcgagagccgccgctgtggcctcgtcaatctacatcaaggcatcaagcaacatcagtttttggcccatttcttgtcagttagcatcagcaaaccatcgtcctctctgatctgcagcagcaacaacaacaactcccgttcacctcctagtagatgcagcagctagcccattgttgtctcctaatttcagcagtagaatttccatgttccattgtttgtgttgcaacatgcattctgcagattgtcatcaagcttgtattccaccacatgtgttagtcagtttttgcgcttgctaatcgctgccatccactactcactcatcaatcatcaccatgctttgttattgttagctacttattataatccaattttacttctccaaattatcatgatggcttgtagtagtcactggttaggtcattagctgcttgcttgctactgtcaacatgttcactagcatcctttgtgtcttttgctagtaatgtgcatctctgcttgcagtccatctatcaatattgcctttttgctatatccatcaatccgtgttctttactaatcaaattcctgtgtttcctaccgagtccagcaaaatccattgcatatccttcattttgtcgaagctgtgccagtccatctcttcatggtcatttcatcatcaccagctccccgtcggagctggccttcattgtcacttcatatcttcatcatctccatgtcggagcttgtcatcatcatcatcaatcagtgaaggtccgaagaacagtttgtgaagggccttcatcaatatcaatgtctcatcatcaatcaaaggaccgaagaacagcttgtgaaggtccattgtcatcatcatcaacatctccgaatcaatgaagagccgaagaacgattcgtgaaggcttcaaggtaccgagggccgaagaacagtttgtgaaggctccgaggtccatttcattgaggagggggtcttttcttcttcatcatccaagaattgacacacttcgacgacatcatcaagactttggagataggctaatatttagattgtgttctcatgtttagtttacctttcaaatgcaatgttattacatacacattgcatttgagtgggggtgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattccccaatttctccaaattgtgacacagctcatctgaatattagagaaatataaatttgccactaacatttaacaatgtcccacttgtctactccctccgtcaaaaaaaaccaaacctaagacttagatgtgatataatctagtatctgtctagattcgttgtactagattatggcttaaatactagattaagttttttttgggacggagggagtatgtctaaccaaaataacacaattgatttgcatattgtgttttgcctcgcattgtgccgaagcaatcttatctgtggcaattggtagagttcttcctctcacaccttctctgcaatacaaacgcatctggctctggtaaccttgcactcctctcttagtatttctttccttcacgctttcttgtctctgcttaagttctctatcgtttggtaccatcatgcattaattcctttttcctctatttcccactccaaacatttccataaccactcacatacacaaaaataaaaaaccagacagagagaaaaaaacttgttttgggagttctgttgttatcttcctcacagaagaaaaatctctccatcaccatctgttcttatgccctgcttctattcattcacttgttcacagctgcagtagtgctgttccatggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaatgtgtttacaatgacaagagggtacaagaacattttgacgtcaggatatgggtctgcatctcacgcaaacttgatgttcatcgccatacacgtgagattatcgagtcagcaacaaatggagagtgccctcgtgtggacaatcttgatactctccagtgcagattgaaggacataatgcaaaaatcagagaaattcctgcttgtgttggatgacgtctggtttgatgaatccgtcaatgagagggaatgggaccagttgcttgatcctcttgtttctcagcaggaggggagcagagttttggtgacttctagacgagatgtacttccagctgctcttcactgtaaggatgttgttcatttggaaaacatggaagatgccgagttcttggctctattcaaataccatgctttctctggaacagaaatcagaaatccacagttgcatgcacggctagaagaggtcgcagagaagattgctaaaaggttaggacaatcgcctttagcagcaaggacagtaggctcccagctgagcaggaataaggatattgccatatggaaaagtgctctaaatattgaaaacttaagtgagcccatgaaagctctgttgtggagttataataaattagattcacgtctgcagagatgttttctgtactgcagcttatttccaaaaggtcacaagtataaaattgatgagatggttgacctttgggtggcagaggggctagttgattcacgcaaccagggggataagagaattgaagatattggcagggattacttcaatgagatggtgtcaggatctttctttcaaccagtttctgaaagatatatgggtacatggtacattatgcatgatctgcttcatgatttggcagagtcactgactaaagaagactgcttcagattagaagatgatggggtgaaagagataccagccactgttcgacatctatctatttgtgttgacagtatgaaattccataagcaaaagatctgcaagctacgttatttacgcactgttatctgcattgaccctctcatggatgacggagatgatatttttaatcagctattgaagaatctgaagaagctacgtgtactacatttgtcgttttacaacagtagcagcttgcctgaatgtattggtgagctgaagcaccttcgatatttgagtatcatcagcacattgatttctgaactgccaagatcattgtgtactcttttccacttggagctacttcatttgaatgacaaggtcaagaatttgccggacagactctgtaatttaagaaagttacgacgtcttgaagcatatgatgacagaaacagaatgtataaattgtatagagcagctctccctcaaattccttacataggcaagctatctttgctgcaagatattgatggattttgcgtgcaaaagcagaagggatatgagttgcgacaactgagggacatgaacaagctcggtggtaatttgcgtgtcgttaatcttgaaaatgtaactggaaaggatgaagcctcagagtcgaagttgcatcagaaaactcatcttagaggtttgcatctctcctggaatgatgtggatgacatggatgtttcacatttggagattctagaaggtttgaggccgccatctcaattggaggaccttacaatcgaaggttacaaatccaccatgtacccaagctggttacttgatggatcctattttgagaatctagagtcttttacgcttgctaattgctgtgtgataggaagcctaccacccaataccgagatctttaggcactgtatgacacttacccttgagaatgtcccgaatatgaagacactaccttttcttccagaaggtctcacaagcctatcaattgaggggtgcccactgcttgtgttcactactaataacgatgaactggaacaccatgattatagggagagcatcacgagggcaaacaacctggaaacacaacttgttttgatatgggaagcgaattccgattcagatattaggagcacattgtcgtccgaacactcatccatgaagaagttgacggaattgatggatactgatatgtcgggaaatcttcaaaccattgaaagcgctttagaaatagagagagatgaagccttggttaaagaagatatcatcaaagtatggctctgttgccacgaggagaggatgagattcatttattcaaggaaggctgggttgccgttggttctaccatctggactatgcgtactctctctttcctcctgtagtattacagatggagctttagctatttgccttggtgggcttacttcacttagaaatttgttcttaacagagattatgactttaactacgcttccaccggaagaggtcttccaacatttgggcaatcttagatacttggtcattcgttcctgctggtgtctcagatcattcgggggcttgcgatctgctacctctctttcagaaataagattattttcctgtccttctttacagttggcacgtggcgcagaatttatgcaaatgtcccttgagaagctatgtgtatacaactgtgtgctttcagctgacttcttctgcggtgactggccacatctgaataatattgggttatgtgggtgtagaagctctgcgtccttgtatgttggtgatcttacctccctcaaatcattctcactatatcatttgccagatttatgcgtgctggaaggtttgtcttacctacagcttcaccacgtgcatttgatagatgttccaaagcttactaccgagagcatctcacagttccgcgtacagcgttcgctctatattagcagttccgttatgctcaaccacatgatctccgctgaaggtttcaaagttccagggtttctctctcttgaaagctgcaagaagccatccgtttcattcgaagaatctgcaaatttcacatccgtcaagtgcctgaggttatgtaattgtgaaatgaggtcactaccaggaaatatgaagtgcctatccagtctaacgaaactcgatatctatgactgccccaacataacatctctaccagatctgccgtcctccctccagcatatatgtatatggggttgtgagcttttgaagaagagctgcagggcacctgatggagaaagctggccaaagattgcgcatatccgctggaaggaattcagatgaattgtgcttcagaatcactactacggagaagcgcaaaggtgaaggtcccatcaatgcattatcagaagtactctatgtaatatttatttcattcgctcatgatcttacgaacacatcattaacttcacgtttcctttgttttgcagacaccccaagatttgctctctcaggtaacttcgtctcttcgccacctgctacaaggtagagcgcctcatgctcatatagcagtttcagctctctcctgtagtcctgtggtctgtccggttgtgctgtccactctgttccctgcttcgtcgattgccacatttacgtttccctgctgccttctaccgacccacgcctcccttcatccggtctctgccactgacggcccatgatcgtgtccttttgctttcaccccttaaatggagtcttgttggtccaagaaaatctctgtttgtctgttgaacactcaactatctctcgtattttgtgtgatatcgctcgtatttgattgcgtgatgtgataatatattgaagtaattggattttgtacttcgcac</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185663.1 RefSeq:Os01g0782100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

Os01g0782100

2014-05-24T16:37:08Z

Liangdan2013:

Please input one-sentence summary here.
This rice blast resistance genes was found by two Japanese scholars Imbe and Matsumoto in 1985, they called this gene Pi-sh,this gene has the moderate resistance to the blast fungus strain Kyu77-07A in rice variety Shin-2.this gene has been characterized by using the method of classical genetic.And this gene is linking to pi-t.
==Annotated Information==
===Function===
The genes are accounting for the moderate resistance of the rice variety Shin 2 and some other varieties to the blast fungus strain Kyu 77-07A. Aichi Asahi (Pi-a) and carried out in the present study. Yashiro-mochi (Pi-ta), were found to be susceptible to the strain, while Ishikari Shirol*.e (Pi-i), Kanto 51 (Pi-k), Tsuyuake (Pi-k'!b), Fukunishiki (Pi-z) and Toride I (Pi-zt) were resistant, and Shin 2 (Pi-ks) and Pi N0.4 (Pi-ta2) moderately resistant. Therefore, Kyu 77-07A was classified as one of the strains belonging to race 102. Howver, the variety Reiho with the resistance gene Pi-ta2 was susceptible to this strain. Based on the knovn facts mentioned above, the objectives 0L the present study were' to determinA- whether Pi-ks is the gene responsible for the moderate resistance of Shin 2' to Kyu 77-07A along with the reason for the different reactions to Kyu 77-07A betweerL Pi N0.4 and Reiho which both have the same resistance gene Pi-ta2. For the gene analysis, the F2 or F3 plants of the crosses were inoculated with Kyu 77-07A and three other fungus strains using the spraying rnethod when the plants were at the four- to five-1eaf stage. Two varieties, Mineyutaka. and Saikai 155 belonging to the Shin 2 type varieties, were used as the representative parents of the varieties susceptible to Kyu 77-07A for the crosses.

===Expression===
To identify novel factors required for R gene-mediated resistance in rice, we used rice insertional mutant lines, induced by the endogenous retrotransposon Tos17, in a forward screen involving the rice blast fungus Magnaporthe oryzae. We inoculated 41,119 mutant lines with the fungus using a high throughput procedure, and identified 86 mutant lines with diminished resistance. A genome analysis revealed that 72 of the 86 lines contained mutations in a gene encoding a nucleotide binding site (NB) and leucine rich repeat (LRR) domain-containing (NLR) protein. A genetic complementation analysis and a pathogenesis assay demonstrated that this NLR gene encodes Pish, which confers resistance against races of M. oryzae containing avrPish. The other 14 lines have intact copies of the Pish gene, suggesting that they may contain mutations in the signaling components downstream of Pish. The genome analysis indicated that Pish and its neighboring three NLR genes are high similar to one another and are tandemly located. An in silico analysis of a Tos17 flanking sequence database revealed that this region is a "hot spot" for insertion. Intriguingly, the insertion sites are not distributed evenly among these four NLR genes, despite their similarity at the sequence and expression levels.
In this work we isolated the R gene Pish, and identified several other mutants involved in the signal transduction required for Pish-mediated resistance. These results indicate that our genetic approach is efficient and useful for unveiling novel aspects of defense signaling in rice. Furthermore, our data provide experimental evidence that R gene clusters have the potential to be highly preferred targets for transposable element insertions in plant genomes. Based on this finding, a possible mechanism underlying the high variability of R genes is discussed.
Primers Sequence direction target
AOL45 TCAGAAATCCACAGTTGCATGCAC Forward Npi37-3 and Pish
AOL48 GGAGAGCTGCTCTATACAATTTATAC Reverse Npi37-3 and Pish
AOL51 AGCTGCAGTAGTGCTGTTCCATG Forward Npi37-3 and Pish
AOL52 CGTAAGATCATGAGCGAATG Reverse Pish
AOL53 TGCATGATCATGGGTTAACG Reverse Npi37-3
AOL54 GTGAAAGATTCCACCCAGGTTC Forward Pish
AOL62 TACTCTCTCTTTCCTCCTGTAG Forward Npi37-3 and Pish
AOL64 TCCAAAGCTTACTACCGAGAGC Forward Npi37-2, Npi37-3 and Pish
AOL71 TTGCGCTTCTCCGTAGTAGTG Reverse Pish
AOL72 TTCCGTTTCTCTCTAGTTGTG Reverse Npi37-2 and Npi37-3
AOL212 ACAGCACCCATCAGGCCTAAC Forward Npi37-1
AOL213 GCGGGACGTTACTAGAATTTTG Reverse Npi37-1
AOL233 GCTTCTACACCCACATAACCC Reverse Pish
AOL234 GCTTCTGCACCCAGATAAGAG Reverse Npi37-2 and Npi37-3
AOL351 ACTGCCCCAACATAACATCTC Forward Pish
AOL353 CCTGCAGCTCTTCTTCAAAAG Reverse Pish
AOL356 GACGGAGGGAGTATGTCTAAC Forward Npi37-3 and Pish
AOL357 TTTAACAACGTCCCACTTGTC Forward Npi37-2
AOL369 AACTCAATCTTTTCTCCTGTAG Forward Npi37-2
Tos17-448R CGTTGCTACATCTACCAGTTCG Reverse Tos17
TAIL3 GAGAGCATCATCGGTTACATCTTCTC Forward Tos17
T17-242R CTTAACCTCCTCCAACGAATCG Reverse Tos17
OsAct1U TCCATCTTGGCATCTCTCAG Forward Actin
OsAct1L GTACCCGCATCAGGCATCTG Reverse Actin
===Evolution===
A study was conducted to investigate the relationship between phenotype of blast resistance and resistance gene analog polymorphism of rice, and search molecule hereditary basis of broad spectrum and durable resistance.
【Method】 Comparison of clustering analysis was investigated using spectrum of resistance to blast and polymorphism of resistance gene analog (RGA) in 25 varieties for blast resistance identification and 20 varieties lines).
【Result】 The resistance spectrum clustering analysis showed that the 45 varieties (lines) could be divided into group A and group B with the genetic similarity coefficient of 0.450. Group A and group B could be divided into subclassⅰ, subclass , ⅱ subclass , ⅲ subclass ⅳ, respectively, with 0.618 genetic similarity coefficient. The RGA-PCR clustering analysis showed that proposed the 45 varieties (lines) could be divided into group Ⅰ and group Ⅱ which clearly inclined the Indica-japonica differentiation with 0.620 genetic similarity coefficient. Group Ⅰ could be divided into six subclasses and group Ⅱ could be divided into seven subclasses with 0.783 genetic similarity coefficient. The resistance spectrum clustering analysis showed that some varieties with similar resistance spectrum could finely fall into the same group, while the RGA-PCR clustering analysis showed that some varieties with the similar genetic background could fall into the same group. For some varieties with low resistance frequency or high resistance frequency, there was a better corresponding relationship between the resistance spectrum clustering and the RGA-PCR clustering. General comparison of clustering analysis showed that here was no parallelism relationship between group and group in two different types of the clustering.
【Conclusion】 It could more accurately reflect their enetic background to test resistance to single strain and analysis on RGA polymorphism for resistance parents, and avoid applying the same source of resistance gain and again, and enrich rice resistance germplasm, and breed durable resistance varieties.

You can also add sub-section(s) at will.

==Labs working on this gene==
1.Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Ibaraki, 305-8602，Japan.
2.International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines;
3.Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, Japan;
4.Indonesian Center for Rice Research (ICRR), JL. Raya Muara No. 25A Ciapus Bogor, Subang, West Java, Indonesia;
5.Agricultural Genetics Institute,Conhue, Tuliem, Hanoi, Vietnam;
6.Faculty of Agricultural Sciences, University of Burundi, BP 2940 Bujumbura, Burundi;

==References==
1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta
Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.)
Plant Breeding, 2010, 129(6): 670-675
2. Akira Takahashi;Nagao Hayashi;Akio Miyao;Hirohiko Hirochika
Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging
BMC Plant Biology, 2010, 10: 175
3. Tokio IMBE;Shohei MATSUMOTO
Inheritance of Resrstance of Rice Vanetles to the Blast Fungus Strains Virulent to the Variety "Reiho"
Japanese Journal of Breeding, 1985, 35(0): 332-339.
4.鄂志国, 张丽靖, 焦桂爱, 等. 稻瘟病抗性基因的鉴定及利用进展[J]. 中国水稻科学, 2008, 22(5): 533-540.
5.杨勤忠, 林菲, 冯淑杰, 等. 水稻稻瘟病抗性基因的分子定位及克隆研究进展[J]. 中国农业科学, 2009, 42(5): 1601-1615.
6.任鄄胜, 肖培村, 陈勇, 等. 水稻品种稻瘟病抗性和抗病基因同源序列多态性分析[J]. 中国农业科学, 2009, 42(1): 1-9.
==Structured Information==
{{JaponicaGene|
GeneName = Os01g0782100|
Description = Similar to NBS-LRR protein (Fragment)|
Version = NM_001185663.1 GI:297720460 GeneID:9271927|
Length = 8696 bp|
Definition = Oryza sativa Japonica Group Os01g0782100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:34892692..34901387|
CDS = 34896973..34900845|
GCID = <gbrowseImage1>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:34892692..34901387
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaatgtgtttacaatgacaagagggtacaagaacattttgacgtcaggatatgggtctgcatctcacgcaaacttgatgttcatcgccatacacgtgagattatcgagtcagcaacaaatggagagtgccctcgtgtggacaatcttgatactctccagtgcagattgaaggacataatgcaaaaatcagagaaattcctgcttgtgttggatgacgtctggtttgatgaatccgtcaatgagagggaatgggaccagttgcttgatcctcttgtttctcagcaggaggggagcagagttttggtgacttctagacgagatgtacttccagctgctcttcactgtaaggatgttgttcatttggaaaacatggaagatgccgagttcttggctctattcaaataccatgctttctctggaacagaaatcagaaatccacagttgcatgcacggctagaagaggtcgcagagaagattgctaaaaggttaggacaatcgcctttagcagcaaggacagtaggctcccagctgagcaggaataaggatattgccatatggaaaagtgctctaaatattgaaaacttaagtgagcccatgaaagctctgttgtggagttataataaattagattcacgtctgcagagatgttttctgtactgcagcttatttccaaaaggtcacaagtataaaattgatgagatggttgacctttgggtggcagaggggctagttgattcacgcaaccagggggataagagaattgaagatattggcagggattacttcaatgagatggtgtcaggatctttctttcaaccagtttctgaaagatatatgggtacatggtacattatgcatgatctgcttcatgatttggcagagtcactgactaaagaagactgcttcagattagaagatgatggggtgaaagagataccagccactgttcgacatctatctatttgtgttgacagtatgaaattccataagcaaaagatctgcaagctacgttatttacgcactgttatctgcattgaccctctcatggatgacggagatgatatttttaatcagctattgaagaatctgaagaagctacgtgtactacatttgtcgttttacaacagtagcagcttgcctgaatgtattggtgagctgaagcaccttcgatatttgagtatcatcagcacattgatttctgaactgccaagatcattgtgtactcttttccacttggagctacttcatttgaatgacaaggtcaagaatttgccggacagactctgtaatttaagaaagttacgacgtcttgaagcatatgatgacagaaacagaatgtataaattgtatagagcagctctccctcaaattccttacataggcaagctatctttgctgcaagatattgatggattttgcgtgcaaaagcagaagggatatgagttgcgacaactgagggacatgaacaagctcggtggtaatttgcgtgtcgttaatcttgaaaatgtaactggaaaggatgaagcctcagagtcgaagttgcatcagaaaactcatcttagaggtttgcatctctcctggaatgatgtggatgacatggatgtttcacatttggagattctagaaggtttgaggccgccatctcaattggaggaccttacaatcgaaggttacaaatccaccatgtacccaagctggttacttgatggatcctattttgagaatctagagtcttttacgcttgctaattgctgtgtgataggaagcctaccacccaataccgagatctttaggcactgtatgacacttacccttgagaatgtcccgaatatgaagacactaccttttcttccagaaggtctcacaagcctatcaattgaggggtgcccactgcttgtgttcactactaataacgatgaactggaacaccatgattatagggagagcatcacgagggcaaacaacctggaaacacaacttgttttgatatgggaagcgaattccgattcagatattaggagcacattgtcgtccgaacactcatccatgaagaagttgacggaattgatggatactgatatgtcgggaaatcttcaaaccattgaaagcgctttagaaatagagagagatgaagccttggttaaagaagatatcatcaaagtatggctctgttgccacgaggagaggatgagattcatttattcaaggaaggctgggttgccgttggttctaccatctggactatgcgtactctctctttcctcctgtagtattacagatggagctttagctatttgccttggtgggcttacttcacttagaaatttgttcttaacagagattatgactttaactacgcttccaccggaagaggtcttccaacatttgggcaatcttagatacttggtcattcgttcctgctggtgtctcagatcattcgggggcttgcgatctgctacctctctttcagaaataagattattttcctgtccttctttacagttggcacgtggcgcagaatttatgcaaatgtcccttgagaagctatgtgtatacaactgtgtgctttcagctgacttcttctgcggtgactggccacatctgaataatattgggttatgtgggtgtagaagctctgcgtccttgtatgttggtgatcttacctccctcaaatcattctcactatatcatttgccagatttatgcgtgctggaaggtttgtcttacctacagcttcaccacgtgcatttgatagatgttccaaagcttactaccgagagcatctcacagttccgcgtacagcgttcgctctatattagcagttccgttatgctcaaccacatgatctccgctgaaggtttcaaagttccagggtttctctctcttgaaagctgcaagaagccatccgtttcattcgaagaatctgcaaatttcacatccgtcaagtgcctgaggttatgtaattgtgaaatgaggtcactaccaggaaatatgaagtgcctatccagtctaacgaaactcgatatctatgactgccccaacataacatctctaccagatctgccgtcctccctccagcatatatgtatatggggttgtgagcttttgaagaagagctgcagggcacctgatggagaaagctggccaaagattgcgcatatccgctggaaggaattcagatga</cdnaseq>|
AA = <aaseq>MAEVVLAGLRLAATPICVKLLCNASTCLGVDMTRELHELETIII PQFELVIEAAEKGNHRAKLDRWLRELKQAFYNAEDLLDEHEYNILKCKAKHKDSLVKD STQVHDSSISNILKQPMRAVSSRMSNLRPENRKILCQLNELKTMLEKAKEFRELIHLP AGNSLEGPSVPTIVVPVVTSLLPPRVFGRNMDRDRIIHLLTKPMATVSSSVGYSGLAI VAHGGAGKSTLAQCVYNDKRVQEHFDVRIWVCISRKLDVHRHTREIIESATNGECPRV DNLDTLQCRLKDIMQKSEKFLLVLDDVWFDESVNEREWDQLLDPLVSQQEGSRVLVTS RRDVLPAALHCKDVVHLENMEDAEFLALFKYHAFSGTEIRNPQLHARLEEVAEKIAKR LGQSPLAARTVGSQLSRNKDIAIWKSALNIENLSEPMKALLWSYNKLDSRLQRCFLYC SLFPKGHKYKIDEMVDLWVAEGLVDSRNQGDKRIEDIGRDYFNEMVSGSFFQPVSERY MGTWYIMHDLLHDLAESLTKEDCFRLEDDGVKEIPATVRHLSICVDSMKFHKQKICKL RYLRTVICIDPLMDDGDDIFNQLLKNLKKLRVLHLSFYNSSSLPECIGELKHLRYLSI ISTLISELPRSLCTLFHLELLHLNDKVKNLPDRLCNLRKLRRLEAYDDRNRMYKLYRA ALPQIPYIGKLSLLQDIDGFCVQKQKGYELRQLRDMNKLGGNLRVVNLENVTGKDEAS ESKLHQKTHLRGLHLSWNDVDDMDVSHLEILEGLRPPSQLEDLTIEGYKSTMYPSWLL DGSYFENLESFTLANCCVIGSLPPNTEIFRHCMTLTLENVPNMKTLPFLPEGLTSLSI EGCPLLVFTTNNDELEHHDYRESITRANNLETQLVLIWEANSDSDIRSTLSSEHSSMK KLTELMDTDMSGNLQTIESALEIERDEALVKEDIIKVWLCCHEERMRFIYSRKAGLPL VLPSGLCVLSLSSCSITDGALAICLGGLTSLRNLFLTEIMTLTTLPPEEVFQHLGNLR YLVIRSCWCLRSFGGLRSATSLSEIRLFSCPSLQLARGAEFMQMSLEKLCVYNCVLSA DFFCGDWPHLNNIGLCGCRSSASLYVGDLTSLKSFSLYHLPDLCVLEGLSYLQLHHVH LIDVPKLTTESISQFRVQRSLYISSSVMLNHMISAEGFKVPGFLSLESCKKPSVSFEE SANFTSVKCLRLCNCEMRSLPGNMKCLSSLTKLDIYDCPNITSLPDLPSSLQHICIWG CELLKKSCRAPDGESWPKIAHIRWKEFR</aaseq>|
DNA = <dnaseqindica>4282..8154#gggatctcccgccgttcggagacctaggggagcaagggggagactagagaaggttgcgctccatgaagtcatggtgcaccttaggtatcttgaaaggttttaccaagcgacctggttattgaagcgtggagaagaggctccagtgcggcaggagaaagcagatgagtagaggtacgatctcttcaggagaaagcagagcccagctgcccaggtggtggggagagattatcaaatttttcggtggtggtttggcacgggactctgccgcttcgctgccttcaaggctcaagctcagggtgactggcggcatctgacaggttcatttcctcttgcttatgtcttttagttgcaaatcaattaccattggttgtgggtttgaattattgggcgaaatgctcatgcgttggtcttgaaatttagctttttatctatctctatgttttacattataagtcgctttaatttttttctaattatttttttaagtttgatcaagttcatataaaaatgcaacaacattttcaactcaaaacaaatatattaatataatatattcaatgctatatgtaatgaaactaatttagtgttatgaatgttactatatttttttataaagttggttaaatctaaaaaggtttaattaaaaaaaatcaaaactacttataatacaagtaaaatagagagaattgcatagaagtggaagtgtgtcggtgtctgtactggttctactcacctagtcacctccctgccactgctattctgctaatagtgttagagaatacgcacgcacagataatggcaaggacgtcatttagaagagcccactagtgcgtgtcatgttgcttatgtgcaaattttatacactttcttgactgaccaaaggttgtatgcggtcattttttttttctctcggtgctagctttaatatacggaaagccgtttagagaagatggcatgacaataatggagcatatatatataaactagctcgtaccctgcggcgttgctgcgggtagatattgtaattgcaagtgtccagagaataacaaataaaattttccatggaacaatcatgatggccaaattgtataatatatggctgaaagttcacattatctaaaacaaatatttttcgctgcggtcggagtgtatattaagaaggagagagtttaagcaaactgtacaagagaacaatcagagccaaagctcacatacagaaccaggacactggttcgccacacacacatggttcaaacttcaaacattactgggagtctgatatacatttctttcttgtcttcctgcaaatttgtgttggcactagcttcatacagacctcaactctactgatccaagaacaacatgtagggctacatcctacatgtatacccaaatacccaatacaaacacaaaatagacgaagctaccctgcaatgcaatcagtaataagatcaatagttgataccgaagccaccagccagcatttcgggtaaacatagagcaacaatgaatataagcaagtctcttaagaattacgttctcattttgataaactggcaagcacgggtgaattttgcatcaacacccaacggaaatttgattagcattccttgaaatatgtcactgttgatgaaggtaaaaaagtaaacacaaagtgaagcccatagagcttcagtttagttcaggcatcaacatagtttaactcatgaacctccaaattaaataaattatttatttcattattatatatcataccttggtacatttgtcagcctcacggcagtagattttgcccatgttgcagtaagttacacacacaaacaccaaatatgctagtttttaaactattcttgtttcactagatttaattgcatttggttttttactatgctatttcggagttgtttaatttgatagtgggggccatagagtataatgaaaaacagcaaggtaatgagaaccagagcatagtccggctagaatattttaggagtccttttaatgaggtccagcttgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattctccaatttctccaaattgtaacatggtatcagacccttgttccaaaaaccctagccaccaaatccgatcatcaccggcgcaccggcggcggtgagccgccggcgagccatcatcatcgtcctccttcatccctcaacgtttttgtctccaagcagcaagcctgcagttcgtcgtcgtcaccgtgttcctgttcgccggtcgcagccgccgccagttgcggcgccgcgcccgttcccacttctccacccacgcagccacgccgtcgtggtgttcatccacgctgctcgtcgcggggctcgttcttgccgccgttcgagagccgccgctgtggcctcgtcaatctacatcaaggcatcaagcaacatcagtttttggcccatttcttgtcagttagcatcagcaaaccatcgtcctctctgatctgcagcagcaacaacaacaactcccgttcacctcctagtagatgcagcagctagcccattgttgtctcctaatttcagcagtagaatttccatgttccattgtttgtgttgcaacatgcattctgcagattgtcatcaagcttgtattccaccacatgtgttagtcagtttttgcgcttgctaatcgctgccatccactactcactcatcaatcatcaccatgctttgttattgttagctacttattataatccaattttacttctccaaattatcatgatggcttgtagtagtcactggttaggtcattagctgcttgcttgctactgtcaacatgttcactagcatcctttgtgtcttttgctagtaatgtgcatctctgcttgcagtccatctatcaatattgcctttttgctatatccatcaatccgtgttctttactaatcaaattcctgtgtttcctaccgagtccagcaaaatccattgcatatccttcattttgtcgaagctgtgccagtccatctcttcatggtcatttcatcatcaccagctccccgtcggagctggccttcattgtcacttcatatcttcatcatctccatgtcggagcttgtcatcatcatcatcaatcagtgaaggtccgaagaacagtttgtgaagggccttcatcaatatcaatgtctcatcatcaatcaaaggaccgaagaacagcttgtgaaggtccattgtcatcatcatcaacatctccgaatcaatgaagagccgaagaacgattcgtgaaggcttcaaggtaccgagggccgaagaacagtttgtgaaggctccgaggtccatttcattgaggagggggtcttttcttcttcatcatccaagaattgacacacttcgacgacatcatcaagactttggagataggctaatatttagattgtgttctcatgtttagtttacctttcaaatgcaatgttattacatacacattgcatttgagtgggggtgttaagggtagtatgtctaggggtattttagatatttcccatctctctcttgtacaaatatattttgccctttgggcctaatctaattggaacagttcattccccaatttctccaaattgtgacacagctcatctgaatattagagaaatataaatttgccactaacatttaacaatgtcccacttgtctactccctccgtcaaaaaaaaccaaacctaagacttagatgtgatataatctagtatctgtctagattcgttgtactagattatggcttaaatactagattaagttttttttgggacggagggagtatgtctaaccaaaataacacaattgatttgcatattgtgttttgcctcgcattgtgccgaagcaatcttatctgtggcaattggtagagttcttcctctcacaccttctctgcaatacaaacgcatctggctctggtaaccttgcactcctctcttagtatttctttccttcacgctttcttgtctctgcttaagttctctatcgtttggtaccatcatgcattaattcctttttcctctatttcccactccaaacatttccataaccactcacatacacaaaaataaaaaaccagacagagagaaaaaaacttgttttgggagttctgttgttatcttcctcacagaagaaaaatctctccatcaccatctgttcttatgccctgcttctattcattcacttgttcacagctgcagtagtgctgttccatggcggaggtggtgttggctggcttacgtttggcagcaacaccaatctgtgtgaagctcctctgtaatgcttcgacgtgccttggcgtggacatgacgcgtgagctccatgaactggagaccatcatcataccacagttcgagctggtgattgaagcagctgagaaaggaaaccacagagccaagctggacagatggctccgggagctcaaacaagccttctacaatgctgaggaccttctggacgagcatgagtacaacatccttaagtgcaaagcgaaacacaaggattctctagtgaaagattccacccaggttcatgactcttccatcagcaatattttgaagcagcctatgcgtgctgtgtccagtaggatgtccaatctgcgcccagagaacagaaagatactttgccagttgaatgaactgaagaccatgctggagaaagccaaggagttccgtgagctgattcacttacctgctggtaatagtcttgagggtcccagtgtaccaacaattgttgttcctgtagtgacatcgctattgccgccaagagtatttggtcgcaacatggatcgcgatcgcataatacatcttcttactaagccaatggctactgtttctagctcagtcggctactctggtttggccattgttgcacatggaggagcaggaaaatccaccttagcacaat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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001185663.1 RefSeq:Os01g0782100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

Os03g0227700

2014-05-24T04:57:23Z

Liangdan2013:

Please input one-sentence summary here.
''OsDWAR''F4 is expressed in young elongating tissues.
==Annotated Information==
===Function===
Please input function information here.
xxx

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
1. Sakamoto T;Morinaka Y;Ohnishi T;Sunohara H;Fujioka S;Ueguchi Tanaka M;Mizutani M;Sakata K;Takatsuto S;Yoshida S;Tanaka H;Kitano H;Matsuoka M
Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice
Nature Biotechnology, 2006, 24(1): 105-109

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0227700|
Description = Cytochrome P450 family protein|
Version = NM_001055982.2 GI:297600576 GeneID:4332134|
Length = 6529 bp|
Definition = Oryza sativa Japonica Group Os03g0227700, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:6765109..6771637|
CDS = 6765109..6765374,6765543..6765867,6765971..6766123,6768937..6769188,6769337..6769429<br>,6770222..6770300,6770398..6770504,6771392..6771637|
GCID = <gbrowseImage1>
name=NC_008396:6765109..6771637
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:6765109..6771637
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgccatgatggcgtccataaccagcgagctgctcttctttctccccttcatcctccttgccctgctcacgttctacaccaccaccgtggccaaatgccacggcgggcactggtggcgaggtgggacgacgccggcgaagaggaagcggatgaacctgccgcccggcgccgccgggtggccgctcgtcggcgagacgttcggctacctccgcgcccaccccgccacctccgtcggccgcttcatggagcagcacatcgcacggtacgggaagatataccggtcgagcctgttcggggagcggacggtggtgtcggcggacgcggggctcaaccggtacatcctgcagaacgaggggaggctgttcgagtgcagctacccgcgcagcatcggcggcatcctgggcaagtggtccatgctggtcctcgtcggggacccgcaccgcgagatgcgcgccatctccctcaacttcctctcctccgtccgcctccgcgccgtcctcctccccgaggtcgagcgccacaccctcctcgtcctccgcgcctggcccccttcctccaccttctccgctcagcaccaagccaagaagttcacgttcaacctgatggcgaagaacataatgagcatggacccgggggaggaagagacggagcggctgcggcgggagtacatcaccttcatgaagggcgtggtctccgcgccgctcaacctgcccgggacgccctactggaaggctctcaagtcgcgtgctgccattctcggagtaatagagaggaaaatggaagagcgggttgagaagctgagcaaggaggatgcaagcgtagagcaagacgatcttctcggatgggctctgaaacaatctaacctttcaaaagagcaaatcctggacctcttgctgagcttgctcttcgccgggcacgagacgtcgtccatggcgctcgccctcgccatcttcttccttgaaggctgccccaaggctgtccaagaactgagggaggagcatcttgggattgcaaggagacaaaggctaagaggggagtgcaaattgagctgggaagactacaaagagatggttttcacgcaatgtgtcataaacgagacgttgcggctaggaaacgtggtcaggttcctgcaccggaaggtcatcaaggacgtgcactacaagggttatgacattccaagcggatggaagatcctgccggtgttagccgcggtgcatctggactcgtccctgtacgaggacccccagcgcttcaatccctggagatggaagagtagcggatcatccggcggcttggctcagagcagcagcttcatgccgtacggcggcgggacgcggctgtgcgccgggtcggagctcgcgaagctggagatggccgtgttcttgcaccacctggtgctcaacttcaggtgggagctcgccgagccggaccaagccttcgtcttccccttcgtcgacttccccaagggccttcccattagggttcatagaattgcacaggatgatgagcaggagtaa</cdnaseq>|
AA = <aaseq>MAAMMASITSELLFFLPFILLALLTFYTTTVAKCHGGHWWRGGT TPAKRKRMNLPPGAAGWPLVGETFGYLRAHPATSVGRFMEQHIARYGKIYRSSLFGER TVVSADAGLNRYILQNEGRLFECSYPRSIGGILGKWSMLVLVGDPHREMRAISLNFLS SVRLRAVLLPEVERHTLLVLRAWPPSSTFSAQHQAKKFTFNLMAKNIMSMDPGEEETE RLRREYITFMKGVVSAPLNLPGTPYWKALKSRAAILGVIERKMEERVEKLSKEDASVE QDDLLGWALKQSNLSKEQILDLLLSLLFAGHETSSMALALAIFFLEGCPKAVQELREE HLGIARRQRLRGECKLSWEDYKEMVFTQCVINETLRLGNVVRFLHRKVIKDVHYKGYD IPSGWKILPVLAAVHLDSSLYEDPQRFNPWRWKSSGSSGGLAQSSSFMPYGGGTRLCA GSELAKLEMAVFLHHLVLNFRWELAEPDQAFVFPFVDFPKGLPIRVHRIAQDDEQE</aaseq>|
DNA = <dnaseqindica>1..266#435..759#863..1015#3829..4080#4229..4321#5114..5192#5290..5396#6284..6529#atggccgccatgatggcgtccataaccagcgagctgctcttctttctccccttcatcctccttgccctgctcacgttctacaccaccaccgtggccaaatgccacggcgggcactggtggcgaggtgggacgacgccggcgaagaggaagcggatgaacctgccgcccggcgccgccgggtggccgctcgtcggcgagacgttcggctacctccgcgcccaccccgccacctccgtcggccgcttcatggagcagcacatcgcacggtcggtggtttcattactcggtacacgcacacagctatataagcacgcgcactgctcgcctcgccgtgaagcaagcttagctagtagctaggatatcgatcgatcggtaataataggctgacggcgagctggggggtggcgtgcggttgcgtgcgtgcgtgcatgcaggtacgggaagatataccggtcgagcctgttcggggagcggacggtggtgtcggcggacgcggggctcaaccggtacatcctgcagaacgaggggaggctgttcgagtgcagctacccgcgcagcatcggcggcatcctgggcaagtggtccatgctggtcctcgtcggggacccgcaccgcgagatgcgcgccatctccctcaacttcctctcctccgtccgcctccgcgccgtcctcctccccgaggtcgagcgccacaccctcctcgtcctccgcgcctggcccccttcctccaccttctccgctcagcaccaagccaagaaggtacaacacacatcgtcctccattaagttgagcatgcatatatcaatcagctcgctctagcgatcaatatcaatgaaacgcgcgtttgcgtgcgtgcgtgcagttcacgttcaacctgatggcgaagaacataatgagcatggacccgggggaggaagagacggagcggctgcggcgggagtacatcaccttcatgaagggcgtggtctccgcgccgctcaacctgcccgggacgccctactggaaggctctcaaggtacgtactcgtagctactagctactccctccgtttcgaaatgtttgacgccgttgactttttatcacatatttgaccgttcgttttatttaaaaaaattaagtaattattaattattttcctatcatttgattcattgttaaatatatttttatgtaggcatataattttacatatctcacaaaagtttttaaataagacgaacggtcaaacatttgctaaaaagtcaacggtgtcaaatatttcgaaacggaggaagtagctagctaccaccccacccgtgcggattgatcgatcgatcgatgtccctctctgcctacacacaccctgcgccccggccgtacgcgcgcaaagtcgtgcgcgcatgagttttactccttccgtttcaaaaaaaaaacgacctagacaagtaatatgatgtaacctagaacaacgaatctatacaggagtatatccaaattcattgtcctagattacatcatatctttgtctatgttgccttttttaggaggagagagtactttacatatgagttatgaccggccggccaggttcctttcttcccctattccgtatgcatactataagcagctgcatataggcatatactactatacccagcttagctaaagcaaacaacttgcaacgacgatcatgtgcaggtccctgttgacagaggttagagatgtaaaccatttttgttgttttcattatctaaagagaaaacaagttactacgacgatcgagatgtacgcaaggggatcatatttatattggagaggagagagagatgatttgcgacttgtgagtgataagtgaatatatatactatatatttgtgtaggagtgacgatgacttccctgtcaatgttttgtttggtatgcacacggggggaaaagagtttagagaaaggaattggctagagagggccaggtttagtttctttaatttttgtttggtggttttgtgtgtagctagtccgcatgcatgatatatcatatatatgcatggcccatcattagatatatatacatgacggcagtgcacttattatatatatgtaggggcatatacaaggtgtatgtgtttagaattgtggatggtccaggaattgttgcagctagttctatactagcatagtgaattagtgatccatatcgagatcaggtccaatcggtcagctcgcacagcgggtcttttagtacgatttgttcggacaaattatcttgcctttgagataatcaacataggattgaaactgcatactagcaatagaccatagctatttggcaatatagaaagctaaaaaatgttgattgaaaggtgcgtgcatgcatgctagcagtatgtcttttaggccaggtccagtttttttttttttctcaaaggcagttgcttaaggtgaaattctagcagaggtcatatgaatatatttattatcttccaaacatgagccaaaacctatatatagttaactcaaaaaaccatgcattggtacttcttctgagtatgcatcctggggactgatcgatcatgcagattcttttctttctttttccctggggacgtactgatcatcactcatgtgagcataaaatgttgcctactttttactgcatgtgaggtcacactcaaatgatctttattgattgcctcagcatgcatgctgctagctttcacgatctctctccgatacacacaccctgtgctgtgctcaacagcatgcaacctggcaggtggaatttttaatctgttggaaagtaaatgaccaaccttcataatttggcatgattatatgctggggaatctatcatttatcatgattcaaccgctgaccaaactctttgccacacacccgaaccacattaatttcgttaagcggatgattagccagcaaccttcacagtttggttttaattagttaatgaaagggtgcttgtagttttattagctgaagagacactgcagtactcctactgagcgttctccttaattgggaaatagatctctgcagtactatcctttaactttaagtgaataacatgtagctaggtctcacacttatcaggcccacacatcagtgatccaagtcaaatggcaatacaccataggagattcttccactaattggctaacaaaaataaactgtgatcactgatcacagctgctaacactataacacattaatttgcatgtctcatgaaatgccagatgagttggattaattatctatcttatgttcagtgccgaggaatatacaaggaaacatcattggtatagttgtggagttctgagtgcattatctcacccagtacggtcaaattaatcctagtctccgtaaatatggttgtgtgcactcttagttaattagtgtagacccggaaattatactttgtcgagaatgttgttatccgtgtgttaagtttcaagtaccctgcaggcctataactaaacactgccaacatggatgtttaagaatgaattatacatacagcgcacttggaaaagaaaaggggtagctacccagctgaagaacacctctctctcatttgaaggtcgacacaatatgtgtcttttctatatcgaaaggaaacgacattttttctcttcaaaacagaaagctatgacaagagacataatcctagactagtaggcggttaggcaacaccatatctgaatttgataggtgtcccacatattttttttcttttcctgataagaaggaacttttcaccgtgagcttatttttttctgttctatgtcgacattagttccatgatgtttactgtgtatttttactgttcccctgcagtcgcgtgctgccattctcggagtaatagagaggaaaatggaagagcgggttgagaagctgagcaaggaggatgcaagcgtagagcaagacgatcttctcggatgggctctgaaacaatctaacctttcaaaagagcaaatcctggacctcttgctgagcttgctcttcgccgggcacgagacgtcgtccatggcgctcgccctcgccatcttcttccttgaaggctgccccaaggctgtccaagaactgagggtacagagaattacagattcgaaattaacatctactgtcactagctcagtgctacgtttaactgtagagcaacaattgcagaaagcgaaggcatcagaaatgacatgggaatcgattagttacactcacactgacgcgtgacatgcaggaggagcatcttgggattgcaaggagacaaaggctaagaggggagtgcaaattgagctgggaagactacaaagagatggttttcacgcaatgtgtaaggccccccctactttgttccttccgatccctcctccctaaacgctactaatataaatcggtgcaaaggctttgtgctagcgtagctcttgctgttttgtttggtcacttgcgtagagagatagcaacttcccgaaatgtcatgtacgaatggagactgaaagctctgcgaaataattgccgggccacgtactacagtacttacgctccctctcttcagtttggatcgatcgagctaagaaggcgcacgcatgtgtgcattgatcgatagagtcgatctgttctgccttgtaagctagccagctgaggttctttcgccttggaagtttctacagggtgaggcccatcgtcttgcaacgatccaaatgccatgacaagtacctgttgttgtaggcttccagtagcacggcctgcccccaagaaggtatctgaatcttctgcattgctgttcagacggcagagcagaacatgccgaaataccattccagtcagttgcagtatatgtgtgtggtggtatcagataaaggagcagagtaatttgaggccttagaaattcagatgagttgctcgacaattgtcgcctaccgatcgagaataagttgcatgcatgtatgtgatgtaattatgggtcttagtcagcaggatagtttagtcgctgcagtactgtagattgtagtacttgcgttcaaagcagacccaaaacagaacttgcattcaggaaacaaaacatgaaaaaagaaatatacagtacttggcactgatatttcttttgtttgggtccttattgcaggtcataaacgagacgttgcggctaggaaacgtggtcaggttcctgcaccggaaggtcatcaaggacgtgcactacaagggtgagtagctcgatttcactcttcttccctgaatcgacctctcctctatctgacagcagaagctaactgaactgaactgaactgaactgaactgaaggttatgacattccaagcggatggaagatcctgccggtgttagccgcggtgcatctggactcgtccctgtacgaggacccccagcgcttcaatccctggagatggaaggtcagtcgcagtaggattatcagtgaatctaatctattaatctcggtttaattgctgggattcaaggaaaagaaaatactgggacaagcgcggatgtcgctgttgggcagtgtctgtcaatcataagcgaaggggatcatgtcagaatctgcaaaagcagcacaggctaaacgcgtgcttgcctcgagcagcaatcatgcagagaggcaagctccgcgataggaacatgcatggtgaatcactgaacctgatatgaaagtgaatgctcagctcagctcgctcgctcgcgcgcgcatgtgctttctcatttcgccgtgttgcgtgctagctagtcaatggtgttcccacgaattgttttctcttctcatcacacaaagtcagtggtgaaaaccgaggtgcatgtgcggtaatggaacgggggattaggcggcgacgccgtgatggattatgcattcgtgcgagaaaaatccaggagcgttggtccaaatcgcaagcggatcaaaccgaaccaatcagtggacgattggagccctgcagtccatccatcacatctccattcttatccgctgcgactcacggacattagttgttgaggggtgaacgacgcatgtgtgtatgtatgctcgatcgatcgatcgaacaatggatctgtgtgtggtgtagttgccaacgccgacgagattatctgctcgtctgcatgcagccgcgaaaaagacatgtgtccggattatcctgcaggagattgagcaagataatctgctgcgcaaaaactgcagccaatcgatcattccatgacgcgaaaaaaaaaacaataattactactgctgatacgataacttcgcttcgaattgttgacacacaagtgttcttcttttgttcgattgcagagtagcggatcatccggcggcttggctcagagcagcagcttcatgccgtacggcggcgggacgcggctgtgcgccgggtcggagctcgcgaagctggagatggccgtgttcttgcaccacctggtgctcaacttcaggtgggagctcgccgagccggaccaagccttcgtcttccccttcgtcgacttccccaagggccttcccattagggttcatagaattgcacaggatgatgagcaggagtaa</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055982.2 RefSeq:Os03g0227700]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]