RiceWiki - User contributions [en]

Os02g0553200

2014-06-08T08:03:16Z

Luyuanyuan: /* References */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').<ref name="ref1" />

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.).<ref name="ref2" />.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref1" />

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />

[[File:Os02g0553200-1.jpg|frameless|caption|'''Figure 1.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-2.jpg|frameless|caption|'''Figure 2.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-3.jpg|frameless|caption|'''Figure 3.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-4.jpg|frameless|caption|'''Figure 4.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-5.jpg|frameless|caption|'''Figure 5.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-6.jpg|frameless|caption|'''Figure 6.''' ''Expression(from reference) <ref name="ref2" />.'']].

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption|'''Figure 7.''' ''Expression(from reference) <ref name="ref2" />.'']]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').<ref name="ref2" />

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.<ref name="ref1" /> <ref name="ref2" /> <ref name="ref3" />

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.<ref name="ref1" /> <ref name="ref2" /> <ref name="ref3" /> <ref name="ref4" />

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.<ref name="ref3" />

4 Department of Genetics, Federal University of Rio Grande do Sul, Brazil.<ref name="ref5" />

5 Biotechnology Center, Federal University of Rio Grande do Sul, Brazil.<ref name="ref5" />

6 Department of Botany, Federal University of Rio Grande do Sul, Brazil.<ref name="ref5" />

7 Department of Biochemistry and Molecular Biology, Federal University of Ceará, Brazil.<ref name="ref5" />

8 Department of Biophysics, Federal University of Rio Grande do Sul, Brazil.<ref name="ref5" />

9 Laborato´ rio de Gene´ tica Molecular Vegetal, Departamento de Gene´ tica, UFRJ, 21944-970 Rio de Janeiro, Brasil.<ref name="ref6" />

10 Departamento de Bioquı´mica, Instituto de Quı´mica, UFRJ, 21944-970, Rio de Janeiro, Brasil.<ref name="ref6" />

==References==
<references>
* <ref name="ref1">
Chwan-Yang Hong and Ching Huei Kao. NaCl-induced expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice(''Oryza sativa'' L.)seedlings is not associated with osmotic component[J]. Plant Signaling & Behavior, 2008, 3(3): 199-201.
</ref>
* <ref name="ref2">
Chwan-Yang Hong, Yi Ting Hsu, Yu-Chang Tsai, et al. Expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice (''Oryza sativa'' L.)seedlings in response to NaCl [J]. Journal of Experimental Botany, 2007, 58(12): 3273-3283.
</ref>
* <ref name="ref3">
Chwan-Yang Hong, Yun-Yang Chao, Min-Yu Yang, et al. NaCl-induced expression of glutathione reductase in roots of rice (''Oryza sativa'' L.) seedlings is mediated through hydrogen peroxide but not abscisic acid[J]. Plant Soil, 2009, 320: 103-115.
</ref>
* <ref name="ref4">
Ting-Shao Chou, Yun-Yang Chao and Ching Huei Kao. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology, 2012, 169: 478-486.
</ref>
* <ref name="ref5">
Andréia Caverzana, Aurenivia Bonifaciod, Fabricio E.L. Carvalhod, et al. The knockdown of chloroplastic ascorbate peroxidases reveals itsregulatory role in the photosynthesis and protection underphoto-oxidative stress in rice. Plant Science, 2014, 214: 74-87.
</ref>
* <ref name="ref6">
Felipe Karam Teixeira, Larissa Menezes-Benavente, Roge´ rio Margis, et al. Analysis of the Molecular Evolutionary History of the Ascorbate Peroxidase Gene Family: Inferences from the Rice Genome. Journal of Molecular Evolution, 2004, 59: 761-770.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T08:00:41Z

Luyuanyuan: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').<ref name="ref1" />

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.).<ref name="ref2" />.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref1" />

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />

[[File:Os02g0553200-1.jpg|frameless|caption|'''Figure 1.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-2.jpg|frameless|caption|'''Figure 2.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-3.jpg|frameless|caption|'''Figure 3.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-4.jpg|frameless|caption|'''Figure 4.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-5.jpg|frameless|caption|'''Figure 5.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-6.jpg|frameless|caption|'''Figure 6.''' ''Expression(from reference) <ref name="ref2" />.'']].

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption|'''Figure 7.''' ''Expression(from reference) <ref name="ref2" />.'']]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').<ref name="ref2" />

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.<ref name="ref1" /> <ref name="ref2" /> <ref name="ref3" />

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.<ref name="ref1" /> <ref name="ref2" /> <ref name="ref3" /> <ref name="ref4" />

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.<ref name="ref3" />

4 Department of Genetics, Federal University of Rio Grande do Sul, Brazil.<ref name="ref5" />

5 Biotechnology Center, Federal University of Rio Grande do Sul, Brazil.<ref name="ref5" />

6 Department of Botany, Federal University of Rio Grande do Sul, Brazil.<ref name="ref5" />

7 Department of Biochemistry and Molecular Biology, Federal University of Ceará, Brazil.<ref name="ref5" />

8 Department of Biophysics, Federal University of Rio Grande do Sul, Brazil.<ref name="ref5" />

9 Laborato´ rio de Gene´ tica Molecular Vegetal, Departamento de Gene´ tica, UFRJ, 21944-970 Rio de Janeiro, Brasil.<ref name="ref6" />

10 Departamento de Bioquı´mica, Instituto de Quı´mica, UFRJ, 21944-970, Rio de Janeiro, Brasil.<ref name="ref6" />

==References==
<references>
* <ref name="ref1">
Chwan-Yang Hong and Ching Huei Kao. NaCl-induced expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice(''Oryza sativa'' L.)seedlings is not associated with osmotic component[J]. Plant Signaling & Behavior, 2008, 3(3): 199-201.
</ref>
* <ref name="ref2">
Chwan-Yang Hong, Yi Ting Hsu, Yu-Chang Tsai, et al. Expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice (''Oryza sativa'' L.)seedlings in response to NaCl [J]. Journal of Experimental Botany, 2007, 58(12): 3273-3283.
</ref>
* <ref name="ref3">
Chwan-Yang Hong, Yun-Yang Chao, Min-Yu Yang, et al. NaCl-induced expression of glutathione reductase in roots of rice (''Oryza sativa'' L.) seedlings is mediated through hydrogen peroxide but not abscisic acid[J]. Plant Soil, 2009, 320: 103-115.
</ref>
* <ref name="ref4">
Ting-Shao Chou, Yun-Yang Chao and Ching Huei Kao. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology, 2012, 169: 478-486.
</ref>
* <ref name="ref5">
Andréia Caverzana, Aurenivia Bonifaciod, Fabricio E.L. Carvalhod, et al. The knockdown of chloroplastic ascorbate peroxidases reveals itsregulatory role in the photosynthesis and protection underphoto-oxidative stress in rice. Plant Science, 2014, 214: 74-87.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:54:50Z

Luyuanyuan: /* References */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').<ref name="ref1" />

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.).<ref name="ref2" />.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref1" />

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />

[[File:Os02g0553200-1.jpg|frameless|caption|'''Figure 1.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-2.jpg|frameless|caption|'''Figure 2.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-3.jpg|frameless|caption|'''Figure 3.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-4.jpg|frameless|caption|'''Figure 4.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-5.jpg|frameless|caption|'''Figure 5.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-6.jpg|frameless|caption|'''Figure 6.''' ''Expression(from reference) <ref name="ref2" />.'']].

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption|'''Figure 7.''' ''Expression(from reference) <ref name="ref2" />.'']]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').<ref name="ref2" />

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.<ref name="ref1" /> <ref name="ref2" /> <ref name="ref3" />

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.<ref name="ref1" /> <ref name="ref2" /> <ref name="ref3" /> <ref name="ref4" />

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.<ref name="ref3" />

4 Department of Genetics, Federal University of Rio Grande do Sul, Brazil.<ref name="ref5" />

5 Biotechnology Center, Federal University of Rio Grande do Sul, Brazil.<ref name="ref5" />

6 Department of Botany, Federal University of Rio Grande do Sul, Brazil.<ref name="ref5" />

7 Department of Biochemistry and Molecular Biology, Federal University of Ceará, Brazil.<ref name="ref5" />

8 Department of Biophysics, Federal University of Rio Grande do Sul, Brazil.<ref name="ref5" />

==References==
<references>
* <ref name="ref1">
Chwan-Yang Hong and Ching Huei Kao. NaCl-induced expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice(''Oryza sativa'' L.)seedlings is not associated with osmotic component[J]. Plant Signaling & Behavior, 2008, 3(3): 199-201.
</ref>
* <ref name="ref2">
Chwan-Yang Hong, Yi Ting Hsu, Yu-Chang Tsai, et al. Expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice (''Oryza sativa'' L.)seedlings in response to NaCl [J]. Journal of Experimental Botany, 2007, 58(12): 3273-3283.
</ref>
* <ref name="ref3">
Chwan-Yang Hong, Yun-Yang Chao, Min-Yu Yang, et al. NaCl-induced expression of glutathione reductase in roots of rice (''Oryza sativa'' L.) seedlings is mediated through hydrogen peroxide but not abscisic acid[J]. Plant Soil, 2009, 320: 103-115.
</ref>
* <ref name="ref4">
Ting-Shao Chou, Yun-Yang Chao and Ching Huei Kao. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology, 2012, 169: 478-486.
</ref>
* <ref name="ref5">
Andréia Caverzana, Aurenivia Bonifaciod, Fabricio E.L. Carvalhod, et al. The knockdown of chloroplastic ascorbate peroxidases reveals itsregulatory role in the photosynthesis and protection underphoto-oxidative stress in rice. Plant Science, 2014, 214: 74-87.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:49:32Z

Luyuanyuan: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').<ref name="ref1" />

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.).<ref name="ref2" />.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref1" />

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />

[[File:Os02g0553200-1.jpg|frameless|caption|'''Figure 1.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-2.jpg|frameless|caption|'''Figure 2.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-3.jpg|frameless|caption|'''Figure 3.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-4.jpg|frameless|caption|'''Figure 4.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-5.jpg|frameless|caption|'''Figure 5.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-6.jpg|frameless|caption|'''Figure 6.''' ''Expression(from reference) <ref name="ref2" />.'']].

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption|'''Figure 7.''' ''Expression(from reference) <ref name="ref2" />.'']]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').<ref name="ref2" />

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.<ref name="ref1" /> <ref name="ref2" /> <ref name="ref3" />

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.<ref name="ref1" /> <ref name="ref2" /> <ref name="ref3" /> <ref name="ref4" />

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.<ref name="ref3" />

4 Department of Genetics, Federal University of Rio Grande do Sul, Brazil.<ref name="ref5" />

5 Biotechnology Center, Federal University of Rio Grande do Sul, Brazil.<ref name="ref5" />

6 Department of Botany, Federal University of Rio Grande do Sul, Brazil.<ref name="ref5" />

7 Department of Biochemistry and Molecular Biology, Federal University of Ceará, Brazil.<ref name="ref5" />

8 Department of Biophysics, Federal University of Rio Grande do Sul, Brazil.<ref name="ref5" />

==References==
<references>
* <ref name="ref1">
Chwan-Yang Hong and Ching Huei Kao. NaCl-induced expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice(''Oryza sativa'' L.)seedlings is not associated with osmotic component[J]. Plant Signaling & Behavior, 2008, 3(3): 199-201.
</ref>
* <ref name="ref2">
Chwan-Yang Hong, Yi Ting Hsu, Yu-Chang Tsai, et al. Expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice (''Oryza sativa'' L.)seedlings in response to NaCl [J]. Journal of Experimental Botany, 2007, 58(12): 3273-3283.
</ref>
* <ref name="ref3">
Chwan-Yang Hong, Yun-Yang Chao, Min-Yu Yang, et al. NaCl-induced expression of glutathione reductase in roots of rice (''Oryza sativa'' L.) seedlings is mediated through hydrogen peroxide but not abscisic acid[J]. Plant Soil, 2009, 320: 103-115

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:48:05Z

Luyuanyuan: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').<ref name="ref1" />

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.).<ref name="ref2" />.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref1" />

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />

[[File:Os02g0553200-1.jpg|frameless|caption|'''Figure 1.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-2.jpg|frameless|caption|'''Figure 2.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-3.jpg|frameless|caption|'''Figure 3.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-4.jpg|frameless|caption|'''Figure 4.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-5.jpg|frameless|caption|'''Figure 5.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-6.jpg|frameless|caption|'''Figure 6.''' ''Expression(from reference) <ref name="ref2" />.'']].

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption|'''Figure 7.''' ''Expression(from reference) <ref name="ref2" />.'']]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').<ref name="ref2" />

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.<ref name="ref1" /> <ref name="ref2" /> <ref name="ref3" />

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.<ref name="ref1" /> <ref name="ref2" /> <ref name="ref3" />

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.<ref name="ref3" />

4 Department of Genetics, Federal University of Rio Grande do Sul, Brazil.<ref name="ref4" />

5 Biotechnology Center, Federal University of Rio Grande do Sul, Brazil.<ref name="ref4" />

6 Department of Botany, Federal University of Rio Grande do Sul, Brazil.<ref name="ref4" />

7 Department of Biochemistry and Molecular Biology, Federal University of Ceará, Brazil.<ref name="ref4" />

8 Department of Biophysics, Federal University of Rio Grande do Sul, Brazil.<ref name="ref4" />

==References==
<references>
* <ref name="ref1">
Chwan-Yang Hong and Ching Huei Kao. NaCl-induced expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice(''Oryza sativa'' L.)seedlings is not associated with osmotic component[J]. Plant Signaling & Behavior, 2008, 3(3): 199-201.
</ref>
* <ref name="ref2">
Chwan-Yang Hong, Yi Ting Hsu, Yu-Chang Tsai, et al. Expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice (''Oryza sativa'' L.)seedlings in response to NaCl [J]. Journal of Experimental Botany, 2007, 58(12): 3273-3283.
</ref>
* <ref name="ref3">
Chwan-Yang Hong, Yun-Yang Chao, Min-Yu Yang, et al. NaCl-induced expression of glutathione reductase in roots of rice (''Oryza sativa'' L.) seedlings is mediated through hydrogen peroxide but not abscisic acid[J]. Plant Soil, 2009, 320: 103-115

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:47:15Z

Luyuanyuan: /* Knowledge Extension */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').<ref name="ref1" />

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.).<ref name="ref2" />.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref1" />

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />

[[File:Os02g0553200-1.jpg|frameless|caption|'''Figure 1.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-2.jpg|frameless|caption|'''Figure 2.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-3.jpg|frameless|caption|'''Figure 3.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-4.jpg|frameless|caption|'''Figure 4.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-5.jpg|frameless|caption|'''Figure 5.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-6.jpg|frameless|caption|'''Figure 6.''' ''Expression(from reference) <ref name="ref2" />.'']].

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption|'''Figure 7.''' ''Expression(from reference) <ref name="ref2" />.'']]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').<ref name="ref2" />

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.<ref name="ref1" /> <ref name="ref2" /> <ref name="ref3" />

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.<ref name="ref1" /> <ref name="ref2" /> <ref name="ref3" />

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.<ref name="ref3" />

4 Department of Genetics, Federal University of Rio Grande do Sul, Brazil.

5 Biotechnology Center, Federal University of Rio Grande do Sul, Brazil.

6 Department of Botany, Federal University of Rio Grande do Sul, Brazild.

7 Department of Biochemistry and Molecular Biology, Federal University of Ceará, Brazil.

8 Department of Biophysics, Federal University of Rio Grande do Sul, Brazila.

==References==
<references>
* <ref name="ref1">
Chwan-Yang Hong and Ching Huei Kao. NaCl-induced expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice(''Oryza sativa'' L.)seedlings is not associated with osmotic component[J]. Plant Signaling & Behavior, 2008, 3(3): 199-201.
</ref>
* <ref name="ref2">
Chwan-Yang Hong, Yi Ting Hsu, Yu-Chang Tsai, et al. Expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice (''Oryza sativa'' L.)seedlings in response to NaCl [J]. Journal of Experimental Botany, 2007, 58(12): 3273-3283.
</ref>
* <ref name="ref3">
Chwan-Yang Hong, Yun-Yang Chao, Min-Yu Yang, et al. NaCl-induced expression of glutathione reductase in roots of rice (''Oryza sativa'' L.) seedlings is mediated through hydrogen peroxide but not abscisic acid[J]. Plant Soil, 2009, 320: 103-115

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:45:42Z

Luyuanyuan: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').<ref name="ref1" />

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.).<ref name="ref2" />.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref1" />

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />

[[File:Os02g0553200-1.jpg|frameless|caption|'''Figure 1.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-2.jpg|frameless|caption|'''Figure 2.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-3.jpg|frameless|caption|'''Figure 3.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-4.jpg|frameless|caption|'''Figure 4.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-5.jpg|frameless|caption|'''Figure 5.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-6.jpg|frameless|caption|'''Figure 6.''' ''Expression(from reference) <ref name="ref2" />.'']].

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption|'''Figure 7.''' ''Expression(from reference) <ref name="ref2" />.'']]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').<ref name="ref2" /><ref name="ref3" />

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.<ref name="ref1" /> <ref name="ref2" /> <ref name="ref3" />

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.<ref name="ref1" /> <ref name="ref2" /> <ref name="ref3" />

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.<ref name="ref3" />

4 Department of Genetics, Federal University of Rio Grande do Sul, Brazil.

5 Biotechnology Center, Federal University of Rio Grande do Sul, Brazil.

6 Department of Botany, Federal University of Rio Grande do Sul, Brazild.

7 Department of Biochemistry and Molecular Biology, Federal University of Ceará, Brazil.

8 Department of Biophysics, Federal University of Rio Grande do Sul, Brazila.

==References==
<references>
* <ref name="ref1">
Chwan-Yang Hong and Ching Huei Kao. NaCl-induced expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice(''Oryza sativa'' L.)seedlings is not associated with osmotic component[J]. Plant Signaling & Behavior, 2008, 3(3): 199-201.
</ref>
* <ref name="ref2">
Chwan-Yang Hong, Yi Ting Hsu, Yu-Chang Tsai, et al. Expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice (''Oryza sativa'' L.)seedlings in response to NaCl [J]. Journal of Experimental Botany, 2007, 58(12): 3273-3283.
</ref>
* <ref name="ref3">
Chwan-Yang Hong, Yun-Yang Chao, Min-Yu Yang, et al. NaCl-induced expression of glutathione reductase in roots of rice (''Oryza sativa'' L.) seedlings is mediated through hydrogen peroxide but not abscisic acid[J]. Plant Soil, 2009, 320: 103-115

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:43:24Z

Luyuanyuan: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').<ref name="ref1" />

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.).<ref name="ref2" />.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref1" />

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />

[[File:Os02g0553200-1.jpg|frameless|caption|'''Figure 1.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-2.jpg|frameless|caption|'''Figure 2.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-3.jpg|frameless|caption|'''Figure 3.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-4.jpg|frameless|caption|'''Figure 4.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-5.jpg|frameless|caption|'''Figure 5.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-6.jpg|frameless|caption|'''Figure 6.''' ''Expression(from reference) <ref name="ref2" />.'']].

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption|'''Figure 7.''' ''Expression(from reference) <ref name="ref2" />.'']]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').<ref name="ref2" /><ref name="ref3" />

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.<ref name="ref1" /> <ref name="ref2" />

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.<ref name="ref1" /> <ref name="ref2" />

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

4 Department of Genetics, Federal University of Rio Grande do Sul, Brazil.

5 Biotechnology Center, Federal University of Rio Grande do Sul, Brazil.

6 Department of Botany, Federal University of Rio Grande do Sul, Brazild.

7 Department of Biochemistry and Molecular Biology, Federal University of Ceará, Brazil.

8 Department of Biophysics, Federal University of Rio Grande do Sul, Brazila.

==References==
<references>
* <ref name="ref1">
Chwan-Yang Hong and Ching Huei Kao. NaCl-induced expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice(''Oryza sativa'' L.)seedlings is not associated with osmotic component[J]. Plant Signaling & Behavior, 2008, 3(3): 199-201.
</ref>
* <ref name="ref2">
Chwan-Yang Hong, Yi Ting Hsu, Yu-Chang Tsai, et al. Expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice (''Oryza sativa'' L.)seedlings in response to NaCl [J]. Journal of Experimental Botany, 2007, 58(12): 3273-3283.
</ref>
* <ref name="ref3">
Chwan-Yang Hong, Yun-Yang Chao, Min-Yu Yang, et al. NaCl-induced expression of glutathione reductase in roots of rice (''Oryza sativa'' L.) seedlings is mediated through hydrogen peroxide but not abscisic acid[J]. Plant Soil, 2009, 320: 103-115

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:38:30Z

Luyuanyuan: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').<ref name="ref1" />

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.).<ref name="ref2" />.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref1" />

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />

[[File:Os02g0553200-1.jpg|frameless|caption|'''Figure 1.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-2.jpg|frameless|caption|'''Figure 2.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-3.jpg|frameless|caption|'''Figure 3.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-4.jpg|frameless|caption|'''Figure 4.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-5.jpg|frameless|caption|'''Figure 5.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-6.jpg|frameless|caption|'''Figure 6.''' ''Expression(from reference) <ref name="ref2" />.'']].

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption|'''Figure 7.''' ''Expression(from reference) <ref name="ref2" />.'']]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').<ref name="ref2" /><ref name="ref3" />

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

4 Department of Genetics, Federal University of Rio Grande do Sul, Brazil.

5 Biotechnology Center, Federal University of Rio Grande do Sul, Brazil.

6 Department of Botany, Federal University of Rio Grande do Sul, Brazild.

7 Department of Biochemistry and Molecular Biology, Federal University of Ceará, Brazil.

8 Department of Biophysics, Federal University of Rio Grande do Sul, Brazila.

==References==
<references>
* <ref name="ref1">
Chwan-Yang Hong and Ching Huei Kao. NaCl-induced expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice(''Oryza sativa'' L.)seedlings is not associated with osmotic component[J]. Plant Signaling & Behavior, 2008, 3(3): 199-201.
</ref>
* <ref name="ref2">
Chwan-Yang Hong, Yi Ting Hsu, Yu-Chang Tsai, et al. Expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice (''Oryza sativa'' L.)seedlings in response to NaCl [J]. Journal of Experimental Botany, 2007, 58(12): 3273-3283.
</ref>
* <ref name="ref3">
Chwan-Yang Hong, Yun-Yang Chao, Min-Yu Yang, et al. NaCl-induced expression of glutathione reductase in roots of rice (''Oryza sativa'' L.) seedlings is mediated through hydrogen peroxide but not abscisic acid[J]. Plant Soil, 2009, 320: 103-115

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:33:58Z

Luyuanyuan: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').<ref name="ref1" />

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.).<ref name="ref2" />.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref1" />

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />

[[File:Os02g0553200-1.jpg|frameless|caption|'''Figure 1.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-2.jpg|frameless|caption|'''Figure 2.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-3.jpg|frameless|caption|'''Figure 3.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-4.jpg|frameless|caption|'''Figure 4.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-5.jpg|frameless|caption|'''Figure 5.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-6.jpg|frameless|caption|'''Figure 6.''' ''Expression(from reference) <ref name="ref2" />.'']].

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption|'''Figure 7.''' ''Expression(from reference) <ref name="ref2" />.'']]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').<ref name="ref2" /><ref name="ref3" />

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
<references>
* <ref name="ref1">
Chwan-Yang Hong and Ching Huei Kao. NaCl-induced expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice(''Oryza sativa'' L.)seedlings is not associated with osmotic component[J]. Plant Signaling & Behavior, 2008, 3(3): 199-201.
</ref>
* <ref name="ref2">
Chwan-Yang Hong, Yi Ting Hsu, Yu-Chang Tsai, et al. Expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice (''Oryza sativa'' L.)seedlings in response to NaCl [J]. Journal of Experimental Botany, 2007, 58(12): 3273-3283.
</ref>
* <ref name="ref3">
Chwan-Yang Hong, Yun-Yang Chao, Min-Yu Yang, et al. NaCl-induced expression of glutathione reductase in roots of rice (''Oryza sativa'' L.) seedlings is mediated through hydrogen peroxide but not abscisic acid[J]. Plant Soil, 2009, 320: 103-115

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:33:10Z

Luyuanyuan: /* Knowledge Extension */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').<ref name="ref1" />.

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.).<ref name="ref2" />.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref1" />.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />.

[[File:Os02g0553200-1.jpg|frameless|caption|'''Figure 1.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-2.jpg|frameless|caption|'''Figure 2.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-3.jpg|frameless|caption|'''Figure 3.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-4.jpg|frameless|caption|'''Figure 4.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-5.jpg|frameless|caption|'''Figure 5.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-6.jpg|frameless|caption|'''Figure 6.''' ''Expression(from reference) <ref name="ref2" />.'']].

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption|'''Figure 7.''' ''Expression(from reference) <ref name="ref2" />.'']]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').<ref name="ref2" />.<ref name="ref3" />.

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
<references>
* <ref name="ref1">
Chwan-Yang Hong and Ching Huei Kao. NaCl-induced expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice(''Oryza sativa'' L.)seedlings is not associated with osmotic component[J]. Plant Signaling & Behavior, 2008, 3(3): 199-201.
</ref>
* <ref name="ref2">
Chwan-Yang Hong, Yi Ting Hsu, Yu-Chang Tsai, et al. Expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice (''Oryza sativa'' L.)seedlings in response to NaCl [J]. Journal of Experimental Botany, 2007, 58(12): 3273-3283.
</ref>
* <ref name="ref3">
Chwan-Yang Hong, Yun-Yang Chao, Min-Yu Yang, et al. NaCl-induced expression of glutathione reductase in roots of rice (''Oryza sativa'' L.) seedlings is mediated through hydrogen peroxide but not abscisic acid[J]. Plant Soil, 2009, 320: 103-115

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:32:40Z

Luyuanyuan: /* References */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').<ref name="ref1" />.

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.).<ref name="ref2" />.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref1" />.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />.

[[File:Os02g0553200-1.jpg|frameless|caption|'''Figure 1.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-2.jpg|frameless|caption|'''Figure 2.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-3.jpg|frameless|caption|'''Figure 3.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-4.jpg|frameless|caption|'''Figure 4.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-5.jpg|frameless|caption|'''Figure 5.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-6.jpg|frameless|caption|'''Figure 6.''' ''Expression(from reference) <ref name="ref2" />.'']].

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption|'''Figure 7.''' ''Expression(from reference) <ref name="ref2" />.'']]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.<ref name="ref3" />.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').<ref name="ref2" />.

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
<references>
* <ref name="ref1">
Chwan-Yang Hong and Ching Huei Kao. NaCl-induced expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice(''Oryza sativa'' L.)seedlings is not associated with osmotic component[J]. Plant Signaling & Behavior, 2008, 3(3): 199-201.
</ref>
* <ref name="ref2">
Chwan-Yang Hong, Yi Ting Hsu, Yu-Chang Tsai, et al. Expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice (''Oryza sativa'' L.)seedlings in response to NaCl [J]. Journal of Experimental Botany, 2007, 58(12): 3273-3283.
</ref>
* <ref name="ref3">
Chwan-Yang Hong, Yun-Yang Chao, Min-Yu Yang, et al. NaCl-induced expression of glutathione reductase in roots of rice (''Oryza sativa'' L.) seedlings is mediated through hydrogen peroxide but not abscisic acid[J]. Plant Soil, 2009, 320: 103-115

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:32:11Z

Luyuanyuan: /* References */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').<ref name="ref1" />.

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.).<ref name="ref2" />.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref1" />.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />.

[[File:Os02g0553200-1.jpg|frameless|caption|'''Figure 1.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-2.jpg|frameless|caption|'''Figure 2.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-3.jpg|frameless|caption|'''Figure 3.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-4.jpg|frameless|caption|'''Figure 4.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-5.jpg|frameless|caption|'''Figure 5.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-6.jpg|frameless|caption|'''Figure 6.''' ''Expression(from reference) <ref name="ref2" />.'']].

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption|'''Figure 7.''' ''Expression(from reference) <ref name="ref2" />.'']]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.<ref name="ref3" />.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').<ref name="ref2" />.

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
<references>
* <ref name="ref1">
Chwan-Yang Hong and Ching Huei Kao. NaCl-induced expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice(''Oryza sativa'' L.)seedlings is not associated with osmotic component[J]. Plant Signaling & Behavior, 2008, 3(3): 199-201.
</ref>
* <ref name="ref2">
Chwan-Yang Hong, Yi Ting Hsu, Yu-Chang Tsai, et al. Expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice (''Oryza sativa'' L.)seedlings in response to NaCl [J]. Journal of Experimental Botany, 2007, 58(12): 3273-3283.
* <ref name="ref3">
Chwan-Yang Hong, Yun-Yang Chao, Min-Yu Yang, et al. NaCl-induced expression of glutathione reductase in roots of rice (''Oryza sativa'' L.) seedlings is mediated through hydrogen peroxide but not abscisic acid[J]. Plant Soil, 2009, 320: 103-115

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:31:43Z

Luyuanyuan: /* References */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').<ref name="ref1" />.

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.).<ref name="ref2" />.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref1" />.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />.

[[File:Os02g0553200-1.jpg|frameless|caption|'''Figure 1.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-2.jpg|frameless|caption|'''Figure 2.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-3.jpg|frameless|caption|'''Figure 3.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-4.jpg|frameless|caption|'''Figure 4.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-5.jpg|frameless|caption|'''Figure 5.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-6.jpg|frameless|caption|'''Figure 6.''' ''Expression(from reference) <ref name="ref2" />.'']].

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption|'''Figure 7.''' ''Expression(from reference) <ref name="ref2" />.'']]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.<ref name="ref3" />.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').<ref name="ref2" />.

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
<references>
* <ref name="ref1">
Chwan-Yang Hong and Ching Huei Kao. NaCl-induced expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice(''Oryza sativa'' L.)seedlings is not associated with osmotic component[J]. Plant Signaling & Behavior, 2008, 3(3): 199-201.
</ref>
* <ref name="ref2">
Chwan-Yang Hong, Yi Ting Hsu, Yu-Chang Tsai, et al. Expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice (''Oryza sativa'' L.)seedlings in response to NaCl [J]. Journal of Experimental Botany, 2007, 58(12): 3273-3283.
* <ref name="ref">
Chwan-Yang Hong, Yun-Yang Chao, Min-Yu Yang, et al. NaCl-induced expression of glutathione reductase in roots of rice (''Oryza sativa'' L.) seedlings is mediated through hydrogen peroxide but not abscisic acid[J]. Plant Soil, 2009, 320: 103-115

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:29:17Z

Luyuanyuan: /* Knowledge Extension */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').<ref name="ref1" />.

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.).<ref name="ref2" />.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref1" />.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />.

[[File:Os02g0553200-1.jpg|frameless|caption|'''Figure 1.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-2.jpg|frameless|caption|'''Figure 2.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-3.jpg|frameless|caption|'''Figure 3.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-4.jpg|frameless|caption|'''Figure 4.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-5.jpg|frameless|caption|'''Figure 5.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-6.jpg|frameless|caption|'''Figure 6.''' ''Expression(from reference) <ref name="ref2" />.'']].

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption|'''Figure 7.''' ''Expression(from reference) <ref name="ref2" />.'']]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.<ref name="ref3" />.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').<ref name="ref2" />.

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
<references>
* <ref name="ref1">
Chwan-Yang Hong and Ching Huei Kao. NaCl-induced expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice(''Oryza sativa'' L.)seedlings is not associated with osmotic component[J]. Plant Signaling & Behavior, 2008, 3(3): 199-201.
</ref>
* <ref name="ref2">
Chwan-Yang Hong, Yi Ting Hsu, Yu-Chang Tsai, et al. Expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice (''Oryza sativa'' L.)seedlings in response to NaCl [J]. Journal of Experimental Botany, 2007, 58(12): 3273-3283.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:27:48Z

Luyuanyuan: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').<ref name="ref1" />.

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.).<ref name="ref2" />.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref1" />.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />.

[[File:Os02g0553200-1.jpg|frameless|caption|'''Figure 1.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-2.jpg|frameless|caption|'''Figure 2.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-3.jpg|frameless|caption|'''Figure 3.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-4.jpg|frameless|caption|'''Figure 4.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-5.jpg|frameless|caption|'''Figure 5.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-6.jpg|frameless|caption|'''Figure 6.''' ''Expression(from reference) <ref name="ref2" />.'']].

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption|'''Figure 7.''' ''Expression(from reference) <ref name="ref2" />.'']]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').<ref name="ref2" />.

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
<references>
* <ref name="ref1">
Chwan-Yang Hong and Ching Huei Kao. NaCl-induced expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice(''Oryza sativa'' L.)seedlings is not associated with osmotic component[J]. Plant Signaling & Behavior, 2008, 3(3): 199-201.
</ref>
* <ref name="ref2">
Chwan-Yang Hong, Yi Ting Hsu, Yu-Chang Tsai, et al. Expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice (''Oryza sativa'' L.)seedlings in response to NaCl [J]. Journal of Experimental Botany, 2007, 58(12): 3273-3283.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:25:28Z

Luyuanyuan: /* Function */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').<ref name="ref1" />.

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.).<ref name="ref2" />.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref1" />.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />.

[[File:Os02g0553200-1.jpg|frameless|caption|'''Figure 1.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-2.jpg|frameless|caption|'''Figure 2.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-3.jpg|frameless|caption|'''Figure 3.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-4.jpg|frameless|caption|'''Figure 4.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-5.jpg|frameless|caption|'''Figure 5.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-6.jpg|frameless|caption|'''Figure 6.''' ''Expression(from reference) <ref name="ref2" />.'']].

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption|'''Figure 7.''' ''Expression(from reference) <ref name="ref2" />.'']]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
<references>
* <ref name="ref1">
Chwan-Yang Hong and Ching Huei Kao. NaCl-induced expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice(''Oryza sativa'' L.)seedlings is not associated with osmotic component[J]. Plant Signaling & Behavior, 2008, 3(3): 199-201.
</ref>
* <ref name="ref2">
Chwan-Yang Hong, Yi Ting Hsu, Yu-Chang Tsai, et al. Expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice (''Oryza sativa'' L.)seedlings in response to NaCl [J]. Journal of Experimental Botany, 2007, 58(12): 3273-3283.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:24:13Z

Luyuanyuan: /* References */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'')..<ref name="ref1" />.

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref2" />.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />.

[[File:Os02g0553200-1.jpg|frameless|caption|'''Figure 1.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-2.jpg|frameless|caption|'''Figure 2.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-3.jpg|frameless|caption|'''Figure 3.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-4.jpg|frameless|caption|'''Figure 4.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-5.jpg|frameless|caption|'''Figure 5.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-6.jpg|frameless|caption|'''Figure 6.''' ''Expression(from reference) <ref name="ref2" />.'']].

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption|'''Figure 7.''' ''Expression(from reference) <ref name="ref2" />.'']]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
<references>
* <ref name="ref1">
Chwan-Yang Hong and Ching Huei Kao. NaCl-induced expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice(''Oryza sativa'' L.)seedlings is not associated with osmotic component[J]. Plant Signaling & Behavior, 2008, 3(3): 199-201.
</ref>
* <ref name="ref2">
Chwan-Yang Hong, Yi Ting Hsu, Yu-Chang Tsai, et al. Expression of ''ASCORBATE PEROXIDASE 8'' in roots of rice (''Oryza sativa'' L.)seedlings in response to NaCl [J]. Journal of Experimental Botany, 2007, 58(12): 3273-3283.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:11:39Z

Luyuanyuan: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'')..<ref name="ref1" />.

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref2" />.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />.

[[File:Os02g0553200-1.jpg|frameless|caption|'''Figure 1.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-2.jpg|frameless|caption|'''Figure 2.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-3.jpg|frameless|caption|'''Figure 3.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-4.jpg|frameless|caption|'''Figure 4.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-5.jpg|frameless|caption|'''Figure 5.''' ''Expression(from reference) <ref name="ref2" />.'']].
[[File:Os02g0553200-6.jpg|frameless|caption|'''Figure 6.''' ''Expression(from reference) <ref name="ref2" />.'']].

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption|'''Figure 7.''' ''Expression(from reference) <ref name="ref2" />.'']]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:08:03Z

Luyuanyuan: /* Expression */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'')..<ref name="ref1" />.

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref2" />.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots..<ref name="ref2" />.

[[File:Os02g0553200-1.jpg|frameless|caption]].<ref name="ref2" />.
[[File:Os02g0553200-2.jpg|frameless|caption]].<ref name="ref2" />.
[[File:Os02g0553200-3.jpg|frameless|caption]].<ref name="ref2" />.
[[File:Os02g0553200-4.jpg|frameless|caption]].<ref name="ref2" />.
[[File:Os02g0553200-5.jpg|frameless|caption]].<ref name="ref2" />.
[[File:Os02g0553200-6.jpg|frameless|caption]].<ref name="ref2" />.

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption]]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:07:18Z

Luyuanyuan: /* Function */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'')..<ref name="ref1" />.

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref2" />.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

[[File:Os02g0553200-1.jpg|frameless|caption]]
[[File:Os02g0553200-2.jpg|frameless|caption]]
[[File:Os02g0553200-3.jpg|frameless|caption]]
[[File:Os02g0553200-4.jpg|frameless|caption]]
[[File:Os02g0553200-5.jpg|frameless|caption]]
[[File:Os02g0553200-6.jpg|frameless|caption]]

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption]]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:06:55Z

Luyuanyuan: /* Function */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.<ref name="ref1" />.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

[[File:Os02g0553200-1.jpg|frameless|caption]]
[[File:Os02g0553200-2.jpg|frameless|caption]]
[[File:Os02g0553200-3.jpg|frameless|caption]]
[[File:Os02g0553200-4.jpg|frameless|caption]]
[[File:Os02g0553200-5.jpg|frameless|caption]]
[[File:Os02g0553200-6.jpg|frameless|caption]]

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption]]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:02:47Z

Luyuanyuan: /* Knowledge Extension */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

[[File:Os02g0553200-1.jpg|frameless|caption]]
[[File:Os02g0553200-2.jpg|frameless|caption]]
[[File:Os02g0553200-3.jpg|frameless|caption]]
[[File:Os02g0553200-4.jpg|frameless|caption]]
[[File:Os02g0553200-5.jpg|frameless|caption]]
[[File:Os02g0553200-6.jpg|frameless|caption]]

===Knowledge Extension===

[[File:Os02g0553200-7.jpg|thumb|caption]]

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-08T07:02:07Z

Luyuanyuan: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

[[File:Os02g0553200-1.jpg|frameless|caption]]
[[File:Os02g0553200-2.jpg|frameless|caption]]
[[File:Os02g0553200-3.jpg|frameless|caption]]
[[File:Os02g0553200-4.jpg|frameless|caption]]
[[File:Os02g0553200-5.jpg|frameless|caption]]
[[File:Os02g0553200-6.jpg|frameless|caption]]

===Knowledge Extension===

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

[[File:Os02g0553200-7.jpg|thumb|caption]]

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

File:Os02g0553200-7.jpg

2014-06-08T07:00:08Z

Luyuanyuan:

File:Os02g0553200-6.jpg

2014-06-08T06:54:13Z

Luyuanyuan:

File:Os02g0553200-5.jpg

2014-06-08T06:49:22Z

Luyuanyuan:

Os02g0553200

2014-06-08T06:43:50Z

Luyuanyuan: /* Expression */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

[[File:Os02g0553200-1.jpg|frameless|caption]]
[[File:Os02g0553200-2.jpg|frameless|caption]]
[[File:Os02g0553200-3.jpg|frameless|caption]]
[[File:Os02g0553200-4.jpg|frameless|caption]]
[[File:Os02g0553200-5.jpg|frameless|caption]]
[[File:Os02g0553200-6.jpg|frameless|caption]]
[[File:Os02g0553200-7.jpg|frameless|caption]]

===Knowledge Extension===

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

File:Os02g0553200-4.jpg

2014-06-08T06:43:10Z

Luyuanyuan:

File:Os02g0553200-3.jpg

2014-06-08T06:33:31Z

Luyuanyuan:

Os02g0553200

2014-06-08T06:16:19Z

Luyuanyuan: /* Expression */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

[[File:Os02g0553200-1.jpg|frameless|caption]]
[[File:Os02g0553200-2.jpg|frameless|caption]]
[[File:Os02g0553200-3.jpg|frameless|caption]]
[[File:Os02g0553200-4.jpg|frameless|caption]]

===Knowledge Extension===

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

File:Os02g0553200-2.jpg

2014-06-08T06:15:43Z

Luyuanyuan:

Os02g0553200

2014-06-08T06:13:12Z

Luyuanyuan: /* Expression */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

[[File:Os02g0553200-1.jpg|frameless|caption]]
[[File:Os02g0553200-2.jpg|frameless|caption]]

===Knowledge Extension===

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

File:Os02g0553200-1.jpg

2014-06-08T06:12:36Z

Luyuanyuan:

Os02g0553200

2014-06-08T06:12:06Z

Luyuanyuan: /* Expression */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

[[File:Os02g0553200-1.jpg|frameless|caption]]

===Knowledge Extension===

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

File:Lyy2.jpg

2014-06-06T15:53:02Z

Luyuanyuan:

Os02g0553200

2014-06-06T15:52:00Z

Luyuanyuan: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

[[File:Lyy2.jpg|frameless|caption]]

===Knowledge Extension===

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-06T15:51:27Z

Luyuanyuan: /* Evolution */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

[[File:Lyy1.jpg|frameless|caption]]

===Knowledge Extension===

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-06T15:50:51Z

Luyuanyuan: /* Function */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

===Evolution===
Please input evolution information here.

===Knowledge Extension===

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-06T15:49:31Z

Luyuanyuan: /* Function */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.

[[File:lyy1.jpg|thumb|caption]]

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

===Evolution===
Please input evolution information here.

===Knowledge Extension===

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

File:Lyy1.jpg

2014-06-06T13:06:57Z

Luyuanyuan: uploaded a new version of "File:Lyy1.jpg": Fig. 1. Effect of NaCl concentration on mRNA levels for OsAPx genes (A) and APx activities (B) in root of rice seedlings. Two-day-old seedlings were treated with NaCl (0–300 mM) for 8 h. Semi-quan

Fig. 1. Effect of NaCl concentration on mRNA levels for OsAPx genes (A) and APx activities (B) in root of rice seedlings. Two-day-old seedlings were treated with NaCl (0–300 mM) for 8 h. Semi-quantitative RT-PCR for OsAPx genes was performed as described in Materials and methods. The values of mRNA for the OsAPx genes were adjusted by the corresponding amount of OsActin mRNA for equality of loading. After the adjustment by OsActin, the reaction with the roots in 0 mM NaCl was treated as the normalized reference, with a value of one, for determination of the relative amount of mRNA of OsAPx genes. Bars show means 6SE (n¼4–6). Values with the same letter are not significantly different at P <0.05.

File:Lyy1.jpg

2014-06-06T13:01:13Z

Luyuanyuan: Fig. 1. Effect of NaCl concentration on mRNA levels for OsAPx genes (A) and APx activities (B) in root of rice seedlings. Two-day-old seedlings were treated with NaCl (0–300 mM) for 8 h. Semi-quantitative RT-PCR for OsAPx genes was performed as describe

Os02g0553200

2014-06-06T12:58:22Z

Luyuanyuan: /* Function */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.

[[File:lyy1.jpg]]

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

===Evolution===
Please input evolution information here.

===Knowledge Extension===

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-06T12:55:46Z

Luyuanyuan: /* Function */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.

[[File:f1.jpg]]

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

===Evolution===
Please input evolution information here.

===Knowledge Extension===

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-06T12:52:47Z

Luyuanyuan: /* Function */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.

[[F/:f1.jpg|frameless|caption]]

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

===Evolution===
Please input evolution information here.

===Knowledge Extension===

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-06T12:51:54Z

Luyuanyuan: /* Function */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.

[[F:f1.jpg|frameless|caption]]

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

===Evolution===
Please input evolution information here.

===Knowledge Extension===

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-06T12:35:33Z

Luyuanyuan: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').

The effect of Flu on the expression of ''OsAPx8'' and increase in APx activity was reversed by the application of ABA. It appears that NaCl-enhanced expression of ''OsAPx8'' in rice roots is mediated through an accumulation of ABA. Evidence is provided to show that Na+ but not Cl– is required for enhancing ''OsAPx8'' expression, APx activity, and ABA accumulation in rice roots treated with NaCl. H2O2 treatment resulted in an enhancement of ''OsAPx8'' induction but no accumulation of ABA. Diphenylene iodonium treatment, which is known to inhibit NaCl-induced accumulation of H2O2 in rice roots, did not suppress ''OsAPx8'' induction and ABA accumulation by NaCl. It appears that H2O2 is not involved in the regulation of NaCl-induced ''OsAPx8'' expression in rice roots.The results obtained so far seem to conclude that NaCl-inducing ''OsAPx8'' expression in rice roots is associated with ionic but not osmotic component.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

===Evolution===
Please input evolution information here.

===Knowledge Extension===

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-06T12:28:26Z

Luyuanyuan: /* Function */

Please input one-sentence summary here.

==Annotated Information==

===Function===
The results obtained so far seem to conclude that NaCl-inducing OsAPx8 expression in rice roots is associated with ionic but not osmotic component.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

===Evolution===
Please input evolution information here.

===Knowledge Extension===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-06T12:20:41Z

Luyuanyuan: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Please input function information here.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

===Evolution===
Please input evolution information here.

===Knowledge Extension===
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H2O2 in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'' and ''OsAPx8'').

Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]

Os02g0553200

2014-06-06T11:59:56Z

Luyuanyuan: /* Expression */

Please input one-sentence summary here.

==Annotated Information==

===Function===
Please input function information here.

===Expression===

In stress-induced gene expression, ABA has been thought to be a candidate for a signal transducer. The present study indicated that ABA was involved in regulating the expression of ''OsAPx8'' in rice roots by NaCl. This conclusion was based on the following observations:(i) NaCl treatment resulted in an increase in the endogenous level of ABA (Fig. 2B) and the induction of ''OsAPx8'' expression in rice roots (Fig. 2A); (ii) the expression of ''OsAPx8'' in rice roots was enhanced by exogenous ABA (Fig. 3A); (iii) the increase in ABA levels due to NaCl preceded the enhancement of ''OsAPx8'' expression (Fig. 2); (iv) Flu treatment reduced the ABA level, as well as NaCl-induced ''OsAPx8'' expression (Fig.4); and (v) the effect of Flu on the reduction of ''OsAPx8'' expression caused by NaCl can be reversed by the application of ABA (Fig. 4A). The present results suggest that NaCl-enhanced ''OsAPx8'' expression is mediated through ABA accumulation in rice roots.

===Evolution===
Please input evolution information here.

===Knowledge Extension===
Soil salinity, particularly due to NaCl, can be considered as the single most widespread soil toxicity problem that global rice production faces at present. Salinity influences a number of physiological processes. These processes include photosynthesis, nutrient uptake, water absorption, root growth, and cellular metabolism.

Roots play a number of important roles during plant growth and development, and typically are the first and critical part of the plant to encounter soil salinity. When growing in saline soil, roots have to cope with two types of stress. The first of these is an osmotic stress resulting from salt concentration in the soil that results in lowered water potential and a consequent loss of cell turgor in roots. The second is ionic stress induced by changes in the concentrations of Na+, Cl-, or both in the root growing medium and within root tissues. In addition to its known components of osmotic stress and ion toxicity, salt stress is also manifested as an oxidative stress, all of which contribute to its deleterious effects.

The increase in reactive oxygen species (ROS) seems to occur as a response to most, if not all, abiotic stresses including drought and salinity. To minimize and/or to protect against the toxic effects of these damaging ROS, cells have evolved highly regulated enzymatic and non-enzymatic mechanisms to keep a balance between ROS production and destruction in order to maintain cellular redox homeostasis. ROS-scavenging enzymes include superoxide dismutase, ascorbate peroxidase (APx), glutathione reductase, and catalase.

APx (EC 1.11.1.11) belongs to the class I haemcontaining peroxidases found in higher plants and catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. It plays an important role in scavenging and in protecting cells against the toxic effects of H2O2 in higher plants. The fact that APx has a high affinity for H2O2 and is able to detoxify low concentrations of H2O2, whereas catalase has a high reaction rate but a low affinity for H2O2, renders APx an ideal candidate for tight regulation of H2O2. APx is located in different cellular compartments. Eight types of APx have been described for Oryza sativa: two cytosolic (''OsAPx1'' and ''OsAPx2''), two putative peroxisomal (''OsAPx3'' and ''OsAPx4''), and four chloroplastic isoforms (''OsAPx5'', ''OsAPx6'', ''OsAPx7'', and ''OsAPx8'').

==Labs working on this gene==
1 Department of Agricultural Chemistry and Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, China.

3 Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, China.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os02g0553200|
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
Version = NM_001053646.1 GI:115446662 GeneID:4329643|
Length = 3693 bp|
Definition = Oryza sativa Japonica Group Os02g0553200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
AP = Chromosome 2:21726247..21729939|
CDS = 21726478..21726630,21726710..21726937,21727437..21727541,21727616..21727693,21727776..21727850 ,21728505..21728591,21728684..21728751,21728864..21728951,21729051..21729151 ,21729261..21729381,21729476..21729637,21729739..21729909|
GCID = <gbrowseImage1>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008395:21726247..21729939
source=RiceChromosome02
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctga</cdnaseq>|
AA = <aaseq>MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAG GLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREIL KTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLI QPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGP RVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQ SWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDY AEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTP VPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSP DKPLQSNYFLNIMLLIGGLAFLTSLLGS</aaseq>|
DNA = <dnaseqindica>3310..3462#3003..3230#2399..2503#2247..2324#2090..2164#1349..1435#1189..1256#989..1076#789..889#559..679#303..464#31..201#aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggttcgcaatagttttagctcggctcgtgtttttttttttttgggggtggggggggggttgaatcgaggtgttctgagttgactgatgcgtggcacctgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtacggaacgcccgcgccatctcagctctcgcatccatatgaacagagaagagtcagagatgatgaaatcctgcgatcctgctaccatgtacaggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtacttaatttctcgtgtctcggcagtgcaatttcgaattcaggaaattcgtggagtaatcgtgctgatttccttcataatcgtatggttttggttggaattttgataggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggtctcagacttcttctctctgctctagtgactttgctagatattttttggatgatcggtagtctatgtgcttgaatcttttgagaaaaatttcttgcaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctggtcagtgtttccattaagttcttcattctgtttctgatcaacgtttgcatttatgaattgtgagaggaacttatcaaagagtgaccttctggtgttgataaatgccccccagatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggtgtggatgatgcaaaaaagaaattagttgcctatctgctatttgttcttcatgtatgttgtataaccatttcttttggactatttatgcaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactgtatgttttcttgtccactttggaaactcaacaggaaaaataagttattttcagcagtaggaagttctaggcatggccagaccttcaatgttctacactctgggtatagaatattacatttttggggcatttgcaaatttgccaccggttttttcaatattgcaaggatgctactcgaatgacagttctagtgatatttttgcaattttctagtggcagttttgcaataacgattcaaagtagtggtaaatttgcaattgcccctacattttttgagacattcaggcagaggtgaaaacaatgcatgtacttttttgtgttatcttgtaagtaagcaccatattatgcttttaaaatccgtgaatactttgtccttatgctgtaaataaataatgttttaattcaggattttacaaaatgggttatccttacgtttcttttgtatgcatataatggtaaaatagaattaggatgctaagaacttcatagctaacaactttgtcattctctaagccctgatacttctctgtggggaaatgaagattaacttttcgatgcaatcttaccttaatcacaccagcacaatagcaaatcagattagtttcagttttcagactgacatttaaaggtggcttctattattgtggtctacagaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggtgtgttagcattttgtcatggtttacaaaattatttttttcatttgaatatgtgatgattcagctgatgataatgtttcaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggttatttggttgtgctcaatttgtggcttgaatagctcataggtcactgtctgaaatatctgcaacatatgcaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgaggtgaggactaccatctttttttttcattttgatctcctggcaaccaattgtgcctaaacaatcataaacaatacgaaagacacttaagctcttaagttcttttattagaacatgccagattttcttcctcttgtttgcaagttcaaaacatgactccctgattcctcgtccacatcgagtagatatcttttgtaatgtattttttttctagagaattcagtaatgtacttatgtgacagtaagatggagtactggaccttcaggacagaaatatagttcagatagtttttctttttaccgaatgtaatccaaacactgggagtttgaggtagtccatcatatcaatctttcttctgcaatgcaacgaaatggggtttactatggcagcttttcttagctgttcttcatgtaaaaaccagtgcaattgtaggttggatatttacagagtccaactttaccagccttcgatcaggaagtgatggatgtgattttcctgcagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaaggtatgcactctccacgaagcctagcttctagccctagatttgatggacaatgcatggtatcttgcaatggcttttgcagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgact</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053646.1 RefSeq:Os02g0553200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 2]]
[[Category:Chromosome 2]]