http://192.168.164.12:81/ricewiki/api.php?action=feedcontributions&feedformat=atom&user=Maple 2026-10-12T11:59:49Z User contributions MediaWiki 1.30.0 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=183563 2014-06-10T12:01:30Z

<p>Maple: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues.<br /> <br /> Heat stress threatens future prospects of increased grain production in crops. Rice (Oryza sativa) is most important world food crop. The production of rice is getting severely affected with increases in mean global temperature. According to estimates, yield of rice declines by 10% for every 1°C increase in growing period minimum temperature in the dry season &lt;ref name=&quot;ref1&quot; /&gt;. Processes like spikelet fertility, grain quality and yield processes in rice are considered to be especially sensitive to heat stress &lt;ref name=&quot;ref2&quot; /&gt;. For breeding heat tolerant rice, it is important that molecular components that underlie the heat shock response in this species are understood &lt;ref name=&quot;ref3&quot; /&gt;. Microarray profiling data have shown that heat stress response in maturing tomato microspores involves heat shock proteins, ROS scavengers, hormones and sugars &lt;ref name=&quot;ref4&quot; /&gt;. Understandably, the major molecular changes in rice plants as affected by heat stress need to be worked out. This is especially relevant since rice has emerged as a model plant species of the group monocots due to its small genome size, availability of large collection of full-length cDNAs (FLcDNAs) and for the fact that the whole genome of this plant species is completely sequenced. This crop has attracted a great deal of efforts for the elucidation of gene functions; completion of genome sequencing in rice has paved way for comprehensive functional characterization of genes, transcription factors, signaling components and promoters &lt;ref name=&quot;ref5&quot; /&gt;. The completed rice genome sequence has been used for the characterization of a large number of gene families involved in diverse processes and pathways. However, with almost 42000 genes, several of them unknown, there are ample proteins still left to be characterized in this important crop species. In recent years, comprehensive details on heat shock regulated rice HSP20, HSP70, HSP90 and HSF gene families have been reported .<br /> <br /> ===Expression===<br /> HSP100 is a major heat-regulated protein family in diverse organisms. Across the living systems, common features of HSP100 chaperone action include transient interactions with non-native protein species, in the prevention of aggregation and promotion of correct folding and assembly, or in unfolding for translocation or targeting to proteases. Singla and Grover showed that homologues of yeast HSP104protein are expressed in heat shocked rice seedlings&lt;ref name=&quot;ref6&quot; /&gt;. It was subsequently established that apart from heat, rice HSP100 expression is developmentally-controlled as seeds and developing embryos of rice show high constitutive levels of this protein&lt;ref name=&quot;ref7&quot; /&gt;.<br /> <br /> Expression characteristics of plant Hsp100 members.Hsp100 transcript/protein expression is induced during heat stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/protein is induced by desiccation, low temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high level in the tassel at the pre-meiosis stage, the ear (including silks) and the developing endosperm and embryo&lt;ref name=&quot;ref8&quot; /&gt;.<br /> <br /> Detailed analysis of the amino acid sequence has revealed that Hsp100 members contain several conserved signatures. Schirmer divided Hsp100 into class I (Hsp100 types A–D; containing two nucleotide-binding domains) and class II (Hsp100 types M, N, X, and Y; containing one nucleotide-binding domain) proteins. The molecular weights of the Hsp100 proteins is considered to vary between 75 and 100 kDa because of the size of the non-conserved spacers between the 2 domains and additional sequences at the Cand N-termini. Basically, Hsp100 proteins are composed of five specific domains as follows: (i) amino (N) - terminal domain, (ii) nucleotide – binding domain 1 (NBD1), (iii) middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal domain. The conserved sequences of the five domains include (i) signature sequence I in the N-terminal domain, (ii) Walker A, Walker B1, and Walker B2 sequences in the NBD1, (iii) signature sequence II and signature sequence III in the middle domain, (iv) Walker A and Walker B sequences in NBD2 and (v) signature sequence IV and V in the C-terminal domain. Based on more extensive analysis with larger spectrum of plant species, Agarwal reported further specific alterations in the amino acid sequence of various motifs of plant Hsp100 members&lt;ref name=&quot;ref9&quot; /&gt;.<br /> <br /> ===Evolution===<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery&lt;ref name=&quot;ref10&quot; /&gt;.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> [1] Peng S, Huang J, Sheehy JE, Laza RC, Visperas RM, Zhong X, Centeno GS, Khush GS, Cassman KG: Rice yields decline with higher night temperature from global warming. Proc Natl Acad Sci 2004, 101(27):9971-9975.<br /> <br /> [2] Grover A, Chandramouli A, Agarwal S, Katiyar-Agarwal S, Agarwal M, Sahi C: Transgenic rice for tolerance against abiotic stresses. Rice Improvement in the Genomic Era Hawarth Press USADutta SK 2009, 237-267.<br /> <br /> [3] Singh A, Grover A: Genetic engineering for heat tolerance in plants. Physiol Mol Biol Plants 2008, 14:155-166.<br /> <br /> [4] Frank G, Pressman E, Ophir R, Althan L, Shaked R, Freedman M, Shen S, Firon N: Transcriptional profiling of maturing tomato (Solanum lycopersicum L.) microspores reveals the involvement of heat shock proteins, ROS scavengers, hormones, and sugars in the heat stress response. Journal of Experimental Botany 2009, 60(13):3891-3908.<br /> <br /> [5] IRGSP: The map-based sequence of the rice genome. Nature 2005, 436(7052):793-800.<br /> <br /> [6] Singla SL, Grover A: Antibodies raised against yeast HSP 104 cross-react with a heat- and abscisic acid-regulated polypeptide in rice. Plant Molecular Biology 1993, 22(6):1177-1180.<br /> <br /> [7] Pareek A, Singla SL, Grover A: Immunological evidence for accumulation of two high-molecular-weight (104 and 90 kDa) HSPs in response to different stresses in rice and in response to high temperature stress in diverse plant genera. Plant Molecular Biology 1995, 29(2):293-301.<br /> <br /> [8] Young T E, Ling J, Geisler-Lee C J, Tanguay R L, Caldwell C and Gallie D R 2001b Developmental and thermal regulation of the maize heat shock protein, HSP101; Plant Physiol. 127 777–791<br /> <br /> [9] Agarwal M, Katiyar-Agarwal S and Grover A 2002 Plant Hsp100 protein: structure, function and regulation; Plant Sci. 163 397–405<br /> <br /> [10] Gaurav Batra, Vineeta Singh Chauhan: Complexity of rice Hsp100 gene family: lessons from rice genome sequence data; J. Biosci.32 611–619.<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=183557 2014-06-10T11:55:40Z

<p>Maple: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues.<br /> <br /> Heat stress threatens future prospects of increased grain production in crops. Rice (Oryza sativa) is most important world food crop. The production of rice is getting severely affected with increases in mean global temperature. According to estimates, yield of rice declines by 10% for every 1°C increase in growing period minimum temperature in the dry season &lt;ref name=&quot;ref1&quot; /&gt;. Processes like spikelet fertility, grain quality and yield processes in rice are considered to be especially sensitive to heat stress &lt;ref name=&quot;ref2&quot; /&gt;. For breeding heat tolerant rice, it is important that molecular components that underlie the heat shock response in this species are understood &lt;ref name=&quot;ref3&quot; /&gt;. Microarray profiling data have shown that heat stress response in maturing tomato microspores involves heat shock proteins, ROS scavengers, hormones and sugars &lt;ref name=&quot;ref4&quot; /&gt;. Understandably, the major molecular changes in rice plants as affected by heat stress need to be worked out. This is especially relevant since rice has emerged as a model plant species of the group monocots due to its small genome size, availability of large collection of full-length cDNAs (FLcDNAs) and for the fact that the whole genome of this plant species is completely sequenced. This crop has attracted a great deal of efforts for the elucidation of gene functions; completion of genome sequencing in rice has paved way for comprehensive functional characterization of genes, transcription factors, signaling components and promoters &lt;ref name=&quot;ref5&quot; /&gt;. The completed rice genome sequence has been used for the characterization of a large number of gene families involved in diverse processes and pathways. However, with almost 42000 genes, several of them unknown, there are ample proteins still left to be characterized in this important crop species. In recent years, comprehensive details on heat shock regulated rice HSP20, HSP70, HSP90 and HSF gene families have been reported .<br /> <br /> ===Expression===<br /> HSP100 is a major heat-regulated protein family in diverse organisms. Across the living systems, common features of HSP100 chaperone action include transient interactions with non-native protein species, in the prevention of aggregation and promotion of correct folding and assembly, or in unfolding for translocation or targeting to proteases. Singla and Grover showed that homologues of yeast HSP104protein are expressed in heat shocked rice seedlings&lt;ref name=&quot;ref6&quot; /&gt;. It was subsequently established that apart from heat, rice HSP100 expression is developmentally-controlled as seeds and developing embryos of rice show high constitutive levels of this protein&lt;ref name=&quot;ref7&quot; /&gt;.<br /> <br /> Expression characteristics of plant Hsp100 members.Hsp100 transcript/protein expression is induced during heat stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/protein is induced by desiccation, low temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high level in the tassel at the pre-meiosis stage, the ear (including silks) and the developing endosperm and embryo&lt;ref name=&quot;ref8&quot; /&gt;.<br /> <br /> Detailed analysis of the amino acid sequence has revealed that Hsp100 members contain several conserved signatures. Schirmer divided Hsp100 into class I (Hsp100 types A–D; containing two nucleotide-binding domains) and class II (Hsp100 types M, N, X, and Y; containing one nucleotide-binding domain) proteins. The molecular weights of the Hsp100 proteins is considered to vary between 75 and 100 kDa because of the size of the non-conserved spacers between the 2 domains and additional sequences at the Cand N-termini. Basically, Hsp100 proteins are composed of five specific domains as follows: (i) amino (N) - terminal domain, (ii) nucleotide – binding domain 1 (NBD1), (iii) middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal domain. The conserved sequences of the five domains include (i) signature sequence I in the N-terminal domain, (ii) Walker A, Walker B1, and Walker B2 sequences in the NBD1, (iii) signature sequence II and signature sequence III in the middle domain, (iv) Walker A and Walker B sequences in NBD2 and (v) signature sequence IV and V in the C-terminal domain. Based on more extensive analysis with larger spectrum of plant species, Agarwal reported further specific alterations in the amino acid sequence of various motifs of plant Hsp100 members&lt;ref name=&quot;ref9&quot; /&gt;.<br /> <br /> ===Evolution===<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery&lt;ref name=&quot;ref10&quot; /&gt;.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> [1] Peng S, Huang J, Sheehy JE, Laza RC, Visperas RM, Zhong X, Centeno GS, Khush GS, Cassman KG: Rice yields decline with higher night temperature from global warming. Proc Natl Acad Sci 2004, 101(27):9971-9975.<br /> <br /> [2] Grover A, Chandramouli A, Agarwal S, Katiyar-Agarwal S, Agarwal M, Sahi C: Transgenic rice for tolerance against abiotic stresses. Rice Improvement in the Genomic Era Hawarth Press USADutta SK 2009, 237-267.<br /> <br /> [3] Singh A, Grover A: Genetic engineering for heat tolerance in plants. Physiol Mol Biol Plants 2008, 14:155-166.<br /> <br /> [4] Frank G, Pressman E, Ophir R, Althan L, Shaked R, Freedman M, Shen S, Firon N: Transcriptional profiling of maturing tomato (Solanum lycopersicum L.) microspores reveals the involvement of heat shock proteins, ROS scavengers, hormones, and sugars in the heat stress response. Journal of Experimental Botany 2009, 60(13):3891-3908.<br /> <br /> [5] IRGSP: The map-based sequence of the rice genome. Nature 2005, 436(7052):793-800.<br /> <br /> [6] Singla SL, Grover A: Antibodies raised against yeast HSP 104 cross-react with a heat- and abscisic acid-regulated polypeptide in rice. Plant Molecular Biology 1993, 22(6):1177-1180.<br /> <br /> [7] Pareek A, Singla SL, Grover A: Immunological evidence for accumulation of two high-molecular-weight (104 and 90 kDa) HSPs in response to different stresses in rice and in response to high temperature stress in diverse plant genera. Plant Molecular Biology 1995, 29(2):293-301.<br /> <br /> [8] Young T E, Ling J, Geisler-Lee C J, Tanguay R L, Caldwell C and Gallie D R 2001b Developmental and thermal regulation of the maize heat shock protein, HSP101; Plant Physiol. 127 777–791<br /> <br /> [9] Agarwal M, Katiyar-Agarwal S and Grover A 2002 Plant Hsp100 protein: structure, function and regulation; Plant Sci. 163 397–405<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=183552 2014-06-10T11:45:52Z

<p>Maple: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues.<br /> <br /> Heat stress threatens future prospects of increased grain production in crops. Rice (Oryza sativa) is most important world food crop. The production of rice is getting severely affected with increases in mean global temperature. According to estimates, yield of rice declines by 10% for every 1°C increase in growing period minimum temperature in the dry season &lt;ref name=&quot;ref1&quot; /&gt;. Processes like spikelet fertility, grain quality and yield processes in rice are considered to be especially sensitive to heat stress &lt;ref name=&quot;ref2&quot; /&gt;. For breeding heat tolerant rice, it is important that molecular components that underlie the heat shock response in this species are understood &lt;ref name=&quot;ref3&quot; /&gt;. Microarray profiling data have shown that heat stress response in maturing tomato microspores involves heat shock proteins, ROS scavengers, hormones and sugars &lt;ref name=&quot;ref4&quot; /&gt;. Understandably, the major molecular changes in rice plants as affected by heat stress need to be worked out. This is especially relevant since rice has emerged as a model plant species of the group monocots due to its small genome size, availability of large collection of full-length cDNAs (FLcDNAs) and for the fact that the whole genome of this plant species is completely sequenced. This crop has attracted a great deal of efforts for the elucidation of gene functions; completion of genome sequencing in rice has paved way for comprehensive functional characterization of genes, transcription factors, signaling components and promoters &lt;ref name=&quot;ref5&quot; /&gt;. The completed rice genome sequence has been used for the characterization of a large number of gene families involved in diverse processes and pathways. However, with almost 42000 genes, several of them unknown, there are ample proteins still left to be characterized in this important crop species. In recent years, comprehensive details on heat shock regulated rice HSP20, HSP70, HSP90 and HSF gene families have been reported .<br /> <br /> ===Expression===<br /> HSP100 is a major heat-regulated protein family in diverse organisms. Across the living systems, common features of HSP100 chaperone action include transient interactions with non-native protein species, in the prevention of aggregation and promotion of correct folding and assembly, or in unfolding for translocation or targeting to proteases. Singla and Grover showed that homologues of yeast HSP104protein are expressed in heat shocked rice seedlings&lt;ref name=&quot;ref6&quot; /&gt;. It was subsequently established that apart from heat, rice HSP100 expression is developmentally-controlled as seeds and developing embryos of rice show high constitutive levels of this protein&lt;ref name=&quot;ref7&quot; /&gt;.<br /> <br /> Expression characteristics of plant Hsp100 members.Hsp100 transcript/protein expression is induced during heat stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/protein is induced by desiccation, low temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high level in the tassel at the pre-meiosis stage, the ear (including silks) and the developing endosperm and embryo&lt;ref name=&quot;ref8&quot; /&gt;.<br /> <br /> Detailed analysis of the amino acid sequence has revealed that Hsp100 members contain several conserved signatures. Schirmer divided Hsp100 into class I (Hsp100 types A–D; containing two nucleotide-binding domains) and class II (Hsp100 types M, N, X, and Y; containing one nucleotide-binding domain) proteins. The molecular weights of the Hsp100 proteins is considered to vary between 75 and 100 kDa because of the size of the non-conserved spacers between the 2 domains and additional sequences at the Cand N-termini. Basically, Hsp100 proteins are composed of five specific domains as follows: (i) amino (N) - terminal domain, (ii) nucleotide – binding domain 1 (NBD1), (iii) middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal domain. The conserved sequences of the five domains include (i) signature sequence I in the N-terminal domain, (ii) Walker A, Walker B1, and Walker B2 sequences in the NBD1, (iii) signature sequence II and signature sequence III in the middle domain, (iv) Walker A and Walker B sequences in NBD2 and (v) signature sequence IV and V in the C-terminal domain. Based on more extensive analysis with larger spectrum of plant species, Agarwal reported further specific alterations in the amino acid sequence of various motifs of plant Hsp100 members&lt;ref name=&quot;ref9&quot; /&gt;.<br /> <br /> ===Evolution===<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> [1] Peng S, Huang J, Sheehy JE, Laza RC, Visperas RM, Zhong X, Centeno GS, Khush GS, Cassman KG: Rice yields decline with higher night temperature from global warming. Proc Natl Acad Sci 2004, 101(27):9971-9975.<br /> <br /> [2] Grover A, Chandramouli A, Agarwal S, Katiyar-Agarwal S, Agarwal M, Sahi C: Transgenic rice for tolerance against abiotic stresses. Rice Improvement in the Genomic Era Hawarth Press USADutta SK 2009, 237-267.<br /> <br /> [3] Singh A, Grover A: Genetic engineering for heat tolerance in plants. Physiol Mol Biol Plants 2008, 14:155-166.<br /> <br /> [4] Frank G, Pressman E, Ophir R, Althan L, Shaked R, Freedman M, Shen S, Firon N: Transcriptional profiling of maturing tomato (Solanum lycopersicum L.) microspores reveals the involvement of heat shock proteins, ROS scavengers, hormones, and sugars in the heat stress response. Journal of Experimental Botany 2009, 60(13):3891-3908.<br /> <br /> [5] IRGSP: The map-based sequence of the rice genome. Nature 2005, 436(7052):793-800.<br /> <br /> [6] Singla SL, Grover A: Antibodies raised against yeast HSP 104 cross-react with a heat- and abscisic acid-regulated polypeptide in rice. Plant Molecular Biology 1993, 22(6):1177-1180.<br /> <br /> [7] Pareek A, Singla SL, Grover A: Immunological evidence for accumulation of two high-molecular-weight (104 and 90 kDa) HSPs in response to different stresses in rice and in response to high temperature stress in diverse plant genera. Plant Molecular Biology 1995, 29(2):293-301.<br /> <br /> [8] Young T E, Ling J, Geisler-Lee C J, Tanguay R L, Caldwell C and Gallie D R 2001b Developmental and thermal regulation of the maize heat shock protein, HSP101; Plant Physiol. 127 777–791<br /> <br /> [9] Agarwal M, Katiyar-Agarwal S and Grover A 2002 Plant Hsp100 protein: structure, function and regulation; Plant Sci. 163 397–405<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=183551 2014-06-10T11:43:16Z

<p>Maple: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues.<br /> <br /> Heat stress threatens future prospects of increased grain production in crops. Rice (Oryza sativa) is most important world food crop. The production of rice is getting severely affected with increases in mean global temperature. According to estimates, yield of rice declines by 10% for every 1°C increase in growing period minimum temperature in the dry season &lt;ref name=&quot;ref1&quot; /&gt;. Processes like spikelet fertility, grain quality and yield processes in rice are considered to be especially sensitive to heat stress &lt;ref name=&quot;ref2&quot; /&gt;. For breeding heat tolerant rice, it is important that molecular components that underlie the heat shock response in this species are understood &lt;ref name=&quot;ref3&quot; /&gt;. Microarray profiling data have shown that heat stress response in maturing tomato microspores involves heat shock proteins, ROS scavengers, hormones and sugars &lt;ref name=&quot;ref4&quot; /&gt;. Understandably, the major molecular changes in rice plants as affected by heat stress need to be worked out. This is especially relevant since rice has emerged as a model plant species of the group monocots due to its small genome size, availability of large collection of full-length cDNAs (FLcDNAs) and for the fact that the whole genome of this plant species is completely sequenced. This crop has attracted a great deal of efforts for the elucidation of gene functions; completion of genome sequencing in rice has paved way for comprehensive functional characterization of genes, transcription factors, signaling components and promoters &lt;ref name=&quot;ref5&quot; /&gt;. The completed rice genome sequence has been used for the characterization of a large number of gene families involved in diverse processes and pathways. However, with almost 42000 genes, several of them unknown, there are ample proteins still left to be characterized in this important crop species. In recent years, comprehensive details on heat shock regulated rice HSP20, HSP70, HSP90 and HSF gene families have been reported .<br /> <br /> ===Expression===<br /> HSP100 is a major heat-regulated protein family in diverse organisms. Across the living systems, common features of HSP100 chaperone action include transient interactions with non-native protein species, in the prevention of aggregation and promotion of correct folding and assembly, or in unfolding for translocation or targeting to proteases. Singla and Grover showed that homologues of yeast HSP104protein are expressed in heat shocked rice seedlings&lt;ref name=&quot;ref6&quot; /&gt;. It was subsequently established that apart from heat, rice HSP100 expression is developmentally-controlled as seeds and developing embryos of rice show high constitutive levels of this protein&lt;ref name=&quot;ref7&quot; /&gt;.<br /> <br /> Expression characteristics of plant Hsp100 members.Hsp100 transcript/protein expression is induced during heat stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/protein is induced by desiccation, low temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high level in the tassel at the pre-meiosis stage, the ear (including silks) and the developing endosperm and embryo&lt;ref name=&quot;ref8&quot; /&gt;.<br /> <br /> Detailed analysis of the amino acid sequence has revealed that Hsp100 members contain several conserved signatures. Schirmer divided Hsp100 into class I (Hsp100 types A–D; containing two nucleotide-binding domains) and class II (Hsp100 types M, N, X, and Y; containing one nucleotide-binding domain) proteins. The molecular weights of the Hsp100 proteins is considered to vary between 75 and 100 kDa because of the size of the non-conserved spacers between the 2 domains and additional sequences at the Cand N-termini. Basically, Hsp100 proteins are composed of five specific domains as follows: (i) amino (N) - terminal domain, (ii) nucleotide – binding domain 1 (NBD1), (iii) middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal domain. The conserved sequences of the five domains include (i) signature sequence I in the N-terminal domain, (ii) Walker A, Walker B1, and Walker B2 sequences in the NBD1, (iii) signature sequence II and signature sequence III in the middle domain, (iv) Walker A and Walker B sequences in NBD2 and (v) signature sequence IV and V in the C-terminal domain. Based on more extensive analysis with larger spectrum of plant species, Agarwal reported further specific alterations in the amino acid sequence of various motifs of plant Hsp100 members&lt;ref name=&quot;ref9&quot; /&gt;.<br /> <br /> ===Evolution===<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> [1] Peng S, Huang J, Sheehy JE, Laza RC, Visperas RM, Zhong X, Centeno GS, Khush GS, Cassman KG: Rice yields decline with higher night temperature from global warming. Proc Natl Acad Sci 2004, 101(27):9971-9975.<br /> <br /> [2] Grover A, Chandramouli A, Agarwal S, Katiyar-Agarwal S, Agarwal M, Sahi C: Transgenic rice for tolerance against abiotic stresses. Rice Improvement in the Genomic Era Hawarth Press USADutta SK 2009, 237-267.<br /> <br /> [3] Singh A, Grover A: Genetic engineering for heat tolerance in plants. Physiol Mol Biol Plants 2008, 14:155-166.<br /> <br /> [4] Frank G, Pressman E, Ophir R, Althan L, Shaked R, Freedman M, Shen S, Firon N: Transcriptional profiling of maturing tomato (Solanum lycopersicum L.) microspores reveals the involvement of heat shock proteins, ROS scavengers, hormones, and sugars in the heat stress response. Journal of Experimental Botany 2009, 60(13):3891-3908.<br /> <br /> [5] IRGSP: The map-based sequence of the rice genome. Nature 2005, 436(7052):793-800.<br /> <br /> [6] Singla SL, Grover A: Antibodies raised against yeast HSP 104 cross-react with a heat- and abscisic acid-regulated polypeptide in rice. Plant Molecular Biology 1993, 22(6):1177-1180.<br /> <br /> [7] Pareek A, Singla SL, Grover A: Immunological evidence for accumulation of two high-molecular-weight (104 and 90 kDa) HSPs in response to different stresses in rice and in response to high temperature stress in diverse plant genera. Plant Molecular Biology 1995, 29(2):293-301.<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=183508 2014-06-10T09:37:47Z

<p>Maple: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues.<br /> <br /> Heat stress threatens future prospects of increased grain production in crops. Rice (Oryza sativa) is most important world food crop. The production of rice is getting severely affected with increases in mean global temperature. According to estimates, yield of rice declines by 10% for every 1°C increase in growing period minimum temperature in the dry season &lt;ref name=&quot;ref1&quot; /&gt;. Processes like spikelet fertility, grain quality and yield processes in rice are considered to be especially sensitive to heat stress &lt;ref name=&quot;ref2&quot; /&gt;. For breeding heat tolerant rice, it is important that molecular components that underlie the heat shock response in this species are understood &lt;ref name=&quot;ref3&quot; /&gt;. Microarray profiling data have shown that heat stress response in maturing tomato microspores involves heat shock proteins, ROS scavengers, hormones and sugars &lt;ref name=&quot;ref4&quot; /&gt;. Understandably, the major molecular changes in rice plants as affected by heat stress need to be worked out. This is especially relevant since rice has emerged as a model plant species of the group monocots due to its small genome size, availability of large collection of full-length cDNAs (FLcDNAs) and for the fact that the whole genome of this plant species is completely sequenced. This crop has attracted a great deal of efforts for the elucidation of gene functions; completion of genome sequencing in rice has paved way for comprehensive functional characterization of genes, transcription factors, signaling components and promoters &lt;ref name=&quot;ref5&quot; /&gt;. The completed rice genome sequence has been used for the characterization of a large number of gene families involved in diverse processes and pathways. However, with almost 42000 genes, several of them unknown, there are ample proteins still left to be characterized in this important crop species. In recent years, comprehensive details on heat shock regulated rice HSP20, HSP70, HSP90 and HSF gene families have been reported .<br /> <br /> ===Expression===<br /> HSP100 is a major heat-regulated protein family in diverse organisms. Across the living systems, common features of HSP100 chaperone action include transient interactions with non-native protein species, in the prevention of aggregation and promotion of correct folding and assembly, or in unfolding for translocation or targeting to proteases. Singla and Grover showed that homologues of yeast HSP104protein are expressed in heat shocked rice seedlings&lt;ref name=&quot;ref6&quot; /&gt;. It was subsequently established that apart from heat, rice HSP100 expression is developmentally-controlled as seeds and developing embryos of rice show high constitutive levels of this protein&lt;ref name=&quot;ref7&quot; /&gt;.<br /> <br /> Expression characteristics of plant Hsp100 members.Hsp100 transcript/protein expression is induced during heat stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/protein is induced by desiccation, low temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high level in the tassel at the pre-meiosis stage, the ear (including silks) and the developing endosperm and embryo.<br /> <br /> Detailed analysis of the amino acid sequence has revealed that Hsp100 members contain several conserved signatures. Schirmer divided Hsp100 into class I (Hsp100 types A–D; containing two nucleotide-binding domains) and class II (Hsp100 types M, N, X, and Y; containing one nucleotide-binding domain) proteins. The molecular weights of the Hsp100 proteins is considered to vary between 75 and 100 kDa because of the size of the non-conserved spacers between the 2 domains and additional sequences at the Cand N-termini. Basically, Hsp100 proteins are composed of five specific domains as follows: (i) amino (N) - terminal domain, (ii) nucleotide – binding domain 1 (NBD1), (iii) middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal domain. The conserved sequences of the five domains include (i) signature sequence I in the N-terminal domain, (ii) Walker A, Walker B1, and Walker B2 sequences in the NBD1, (iii) signature sequence II and signature sequence III in the middle domain, (iv) Walker A and Walker B sequences in NBD2 and (v) signature sequence IV and V in the C-terminal domain. Based on more extensive analysis with larger spectrum of plant species, Agarwal reported further specific alterations in the amino acid sequence of various motifs of plant Hsp100 members.<br /> <br /> ===Evolution===<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> [1] Peng S, Huang J, Sheehy JE, Laza RC, Visperas RM, Zhong X, Centeno GS, Khush GS, Cassman KG: Rice yields decline with higher night temperature from global warming. Proc Natl Acad Sci 2004, 101(27):9971-9975.<br /> <br /> [2] Grover A, Chandramouli A, Agarwal S, Katiyar-Agarwal S, Agarwal M, Sahi C: Transgenic rice for tolerance against abiotic stresses. Rice Improvement in the Genomic Era Hawarth Press USADutta SK 2009, 237-267.<br /> <br /> [3] Singh A, Grover A: Genetic engineering for heat tolerance in plants. Physiol Mol Biol Plants 2008, 14:155-166.<br /> <br /> [4] Frank G, Pressman E, Ophir R, Althan L, Shaked R, Freedman M, Shen S, Firon N: Transcriptional profiling of maturing tomato (Solanum lycopersicum L.) microspores reveals the involvement of heat shock proteins, ROS scavengers, hormones, and sugars in the heat stress response. Journal of Experimental Botany 2009, 60(13):3891-3908.<br /> <br /> [5] IRGSP: The map-based sequence of the rice genome. Nature 2005, 436(7052):793-800.<br /> <br /> [6] Singla SL, Grover A: Antibodies raised against yeast HSP 104 cross-react with a heat- and abscisic acid-regulated polypeptide in rice. Plant Molecular Biology 1993, 22(6):1177-1180.<br /> <br /> [7] Pareek A, Singla SL, Grover A: Immunological evidence for accumulation of two high-molecular-weight (104 and 90 kDa) HSPs in response to different stresses in rice and in response to high temperature stress in diverse plant genera. Plant Molecular Biology 1995, 29(2):293-301.<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=183507 2014-06-10T09:36:56Z

<p>Maple: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues.<br /> <br /> Heat stress threatens future prospects of increased grain production in crops. Rice (Oryza sativa) is most important world food crop. The production of rice is getting severely affected with increases in mean global temperature. According to estimates, yield of rice declines by 10% for every 1°C increase in growing period minimum temperature in the dry season &lt;ref name=&quot;ref1&quot; /&gt;. Processes like spikelet fertility, grain quality and yield processes in rice are considered to be especially sensitive to heat stress &lt;ref name=&quot;ref2&quot; /&gt;. For breeding heat tolerant rice, it is important that molecular components that underlie the heat shock response in this species are understood &lt;ref name=&quot;ref3&quot; /&gt;. Microarray profiling data have shown that heat stress response in maturing tomato microspores involves heat shock proteins, ROS scavengers, hormones and sugars &lt;ref name=&quot;ref4&quot; /&gt;. Understandably, the major molecular changes in rice plants as affected by heat stress need to be worked out. This is especially relevant since rice has emerged as a model plant species of the group monocots due to its small genome size, availability of large collection of full-length cDNAs (FLcDNAs) and for the fact that the whole genome of this plant species is completely sequenced. This crop has attracted a great deal of efforts for the elucidation of gene functions; completion of genome sequencing in rice has paved way for comprehensive functional characterization of genes, transcription factors, signaling components and promoters &lt;ref name=&quot;ref5&quot; /&gt;. The completed rice genome sequence has been used for the characterization of a large number of gene families involved in diverse processes and pathways. However, with almost 42000 genes, several of them unknown, there are ample proteins still left to be characterized in this important crop species. In recent years, comprehensive details on heat shock regulated rice HSP20, HSP70, HSP90 and HSF gene families have been reported .<br /> <br /> ===Expression===<br /> HSP100 is a major heat-regulated protein family in diverse organisms. Across the living systems, common features of HSP100 chaperone action include transient interactions with non-native protein species, in the prevention of aggregation and promotion of correct folding and assembly, or in unfolding for translocation or targeting to proteases. Singla and Grover showed that homologues of yeast HSP104protein are expressed in heat shocked rice seedlings&lt;ref name=&quot;ref6&quot; /&gt;. It was subsequently established that apart from heat, rice HSP100 expression is developmentally-controlled as seeds and developing embryos of rice show high constitutive levels of this protein&lt;ref name=&quot;ref7&quot; /&gt;.<br /> <br /> Expression characteristics of plant Hsp100 members.Hsp100 transcript/protein expression is induced during heat stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/protein is induced by desiccation, low temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high level in the tassel at the pre-meiosis stage, the ear (including silks) and the developing endosperm and embryo.<br /> <br /> Detailed analysis of the amino acid sequence has revealed that Hsp100 members contain several conserved signatures. Schirmer divided Hsp100 into class I (Hsp100 types A–D; containing two nucleotide-binding domains) and class II (Hsp100 types M, N, X, and Y; containing one nucleotide-binding domain) proteins. The molecular weights of the Hsp100 proteins is considered to vary between 75 and 100 kDa because of the size of the non-conserved spacers between the 2 domains and additional sequences at the Cand N-termini. Basically, Hsp100 proteins are composed of five specific domains as follows: (i) amino (N) - terminal domain, (ii) nucleotide – binding domain 1 (NBD1), (iii) middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal domain. The conserved sequences of the five domains include (i) signature sequence I in the N-terminal domain, (ii) Walker A, Walker B1, and Walker B2 sequences in the NBD1, (iii) signature sequence II and signature sequence III in the middle domain, (iv) Walker A and Walker B sequences in NBD2 and (v) signature sequence IV and V in the C-terminal domain. Based on more extensive analysis with larger spectrum of plant species, Agarwal reported further specific alterations in the amino acid sequence of various motifs of plant Hsp100 members.<br /> <br /> ===Evolution===<br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> [1] Peng S, Huang J, Sheehy JE, Laza RC, Visperas RM, Zhong X, Centeno GS, Khush GS, Cassman KG: Rice yields decline with higher night temperature from global warming. Proc Natl Acad Sci 2004, 101(27):9971-9975.<br /> <br /> [2] Grover A, Chandramouli A, Agarwal S, Katiyar-Agarwal S, Agarwal M, Sahi C: Transgenic rice for tolerance against abiotic stresses. Rice Improvement in the Genomic Era Hawarth Press USADutta SK 2009, 237-267.<br /> <br /> [3] Singh A, Grover A: Genetic engineering for heat tolerance in plants. Physiol Mol Biol Plants 2008, 14:155-166.<br /> <br /> [4] Frank G, Pressman E, Ophir R, Althan L, Shaked R, Freedman M, Shen S, Firon N: Transcriptional profiling of maturing tomato (Solanum lycopersicum L.) microspores reveals the involvement of heat shock proteins, ROS scavengers, hormones, and sugars in the heat stress response. Journal of Experimental Botany 2009, 60(13):3891-3908.<br /> <br /> [5] IRGSP: The map-based sequence of the rice genome. Nature 2005, 436(7052):793-800.<br /> <br /> [6] Singla SL, Grover A: Antibodies raised against yeast HSP 104 cross-react with a heat- and abscisic acid-regulated polypeptide in rice. Plant Molecular Biology 1993, 22(6):1177-1180.<br /> <br /> [7] Pareek A, Singla SL, Grover A: Immunological evidence for accumulation of two high-molecular-weight (104 and 90 kDa) HSPs in response to different stresses in rice and in response to high temperature stress in diverse plant genera. Plant Molecular Biology 1995, 29(2):293-301.<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=183459 2014-06-10T09:08:33Z

<p>Maple: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues.<br /> <br /> Heat stress threatens future prospects of increased grain production in crops. Rice (Oryza sativa) is most important world food crop. The production of rice is getting severely affected with increases in mean global temperature. According to estimates, yield of rice declines by 10% for every 1°C increase in growing period minimum temperature in the dry season &lt;ref name=&quot;ref1&quot; /&gt;. Processes like spikelet fertility, grain quality and yield processes in rice are considered to be especially sensitive to heat stress &lt;ref name=&quot;ref2&quot; /&gt;. For breeding heat tolerant rice, it is important that molecular components that underlie the heat shock response in this species are understood &lt;ref name=&quot;ref3&quot; /&gt;. Microarray profiling data have shown that heat stress response in maturing tomato microspores involves heat shock proteins, ROS scavengers, hormones and sugars &lt;ref name=&quot;ref4&quot; /&gt;. Understandably, the major molecular changes in rice plants as affected by heat stress need to be worked out. This is especially relevant since rice has emerged as a model plant species of the group monocots due to its small genome size, availability of large collection of full-length cDNAs (FLcDNAs) and for the fact that the whole genome of this plant species is completely sequenced. This crop has attracted a great deal of efforts for the elucidation of gene functions; completion of genome sequencing in rice has paved way for comprehensive functional characterization of genes, transcription factors, signaling components and promoters &lt;ref name=&quot;ref5&quot; /&gt;. The completed rice genome sequence has been used for the characterization of a large number of gene families involved in diverse processes and pathways. However, with almost 42000 genes, several of them unknown, there are ample proteins still left to be characterized in this important crop species. In recent years, comprehensive details on heat shock regulated rice HSP20, HSP70, HSP90 and HSF gene families have been reported .<br /> <br /> ===Expression===<br /> HSP100 is a major heat-regulated protein family in diverse organisms. Across the living systems, common features of HSP100 chaperone action include transient interactions with non-native protein species, in the prevention of aggregation and promotion of correct folding and assembly, or in unfolding for translocation or targeting to proteases. Singla and Grover showed that homologues of yeast HSP104protein are expressed in heat shocked rice seedlings. It was subsequently established that apart from heat, rice HSP100 expression is developmentally-controlled as seeds and developing embryos of rice show high constitutive levels of this protein.<br /> <br /> Expression characteristics of plant Hsp100 members.Hsp100 transcript/protein expression is induced during heat stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/protein is induced by desiccation, low temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high level in the tassel at the pre-meiosis stage, the ear (including silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> [1] Peng S, Huang J, Sheehy JE, Laza RC, Visperas RM, Zhong X, Centeno GS, Khush GS, Cassman KG: Rice yields decline with higher night temperature from global warming. Proc Natl Acad Sci 2004, 101(27):9971-9975.<br /> <br /> [2] Grover A, Chandramouli A, Agarwal S, Katiyar-Agarwal S, Agarwal M, Sahi C: Transgenic rice for tolerance against abiotic stresses. Rice Improvement in the Genomic Era Hawarth Press USADutta SK 2009, 237-267.<br /> <br /> [3] Singh A, Grover A: Genetic engineering for heat tolerance in plants. Physiol Mol Biol Plants 2008, 14:155-166.<br /> <br /> [4] Frank G, Pressman E, Ophir R, Althan L, Shaked R, Freedman M, Shen S, Firon N: Transcriptional profiling of maturing tomato (Solanum lycopersicum L.) microspores reveals the involvement of heat shock proteins, ROS scavengers, hormones, and sugars in the heat stress response. Journal of Experimental Botany 2009, 60(13):3891-3908.<br /> <br /> [5] IRGSP: The map-based sequence of the rice genome. Nature 2005, 436(7052):793-800.<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=183451 2014-06-10T09:02:26Z

<p>Maple: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues.<br /> <br /> Heat stress threatens future prospects of increased grain production in crops. Rice (Oryza sativa) is most important world food crop. The production of rice is getting severely affected with increases in mean global temperature. According to estimates, yield of rice declines by 10% for every 1°C increase in growing period minimum temperature in the dry season &lt;ref name=&quot;ref1&quot; /&gt;. Processes like spikelet fertility, grain quality and yield processes in rice are considered to be especially sensitive to heat stress &lt;ref name=&quot;ref2&quot; /&gt;. For breeding heat tolerant rice, it is important that molecular components that underlie the heat shock response in this species are understood &lt;ref name=&quot;ref3&quot; /&gt;. Microarray profiling data have shown that heat stress response in maturing tomato microspores involves heat shock proteins, ROS scavengers, hormones and sugars &lt;ref name=&quot;ref4&quot; /&gt;. Understandably, the major molecular changes in rice plants as affected by heat stress need to be worked out. This is especially relevant since rice has emerged as a model plant species of the group monocots due to its small genome size, availability of large collection of full-length cDNAs (FLcDNAs) and for the fact that the whole genome of this plant species is completely sequenced. This crop has attracted a great deal of efforts for the elucidation of gene functions; completion of genome sequencing in rice has paved way for comprehensive functional characterization of genes, transcription factors, signaling components and promoters &lt;ref name=&quot;ref5&quot; /&gt;. The completed rice genome sequence has been used for the characterization of a large number of gene families involved in diverse processes and pathways. However, with almost 42000 genes, several of them unknown, there are ample proteins still left to be characterized in this important crop species. In recent years, comprehensive details on heat shock regulated rice HSP20, HSP70, HSP90 and HSF gene families have been reported .<br /> <br /> ===Expression===<br /> HSP100 is a major heat-regulated protein family in diverse organisms. Across the living systems, common features of HSP100 chaperone action include transient interactions with non-native protein species, in the prevention of aggregation and promotion of correct folding and assembly, or in unfolding for translocation or targeting to proteases. Singla and Grover showed that homologues of yeast HSP104protein are expressed in heat shocked rice seedlings. It was subsequently established that apart from heat, rice HSP100 expression is developmentally-controlled as seeds and developing embryos of rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/protein is induced by desiccation, low temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high level in the tassel at the pre-meiosis stage, the ear (including silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> [1] Peng S, Huang J, Sheehy JE, Laza RC, Visperas RM, Zhong X, Centeno GS, Khush GS, Cassman KG: Rice yields decline with higher night temperature from global warming. Proc Natl Acad Sci 2004, 101(27):9971-9975.<br /> <br /> [2] Grover A, Chandramouli A, Agarwal S, Katiyar-Agarwal S, Agarwal M, Sahi C: Transgenic rice for tolerance against abiotic stresses. Rice Improvement in the Genomic Era Hawarth Press USADutta SK 2009, 237-267.<br /> <br /> [3] Singh A, Grover A: Genetic engineering for heat tolerance in plants. Physiol Mol Biol Plants 2008, 14:155-166.<br /> <br /> [4] Frank G, Pressman E, Ophir R, Althan L, Shaked R, Freedman M, Shen S, Firon N: Transcriptional profiling of maturing tomato (Solanum lycopersicum L.) microspores reveals the involvement of heat shock proteins, ROS scavengers, hormones, and sugars in the heat stress response. Journal of Experimental Botany 2009, 60(13):3891-3908.<br /> <br /> [5] IRGSP: The map-based sequence of the rice genome. Nature 2005, 436(7052):793-800.<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=183447 2014-06-10T09:00:19Z

<p>Maple: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues.<br /> <br /> Heat stress threatens future prospects of increased grain production in crops. Rice (Oryza sativa) is most important world food crop. The production of rice is getting severely affected with increases in mean global temperature. According to estimates, yield of rice declines by 10% for every 1°C increase in growing period minimum temperature in the dry season &lt;ref name=&quot;ref1&quot; /&gt;. Processes like spikelet fertility, grain quality and yield processes in rice are considered to be especially sensitive to heat stress &lt;ref name=&quot;ref2&quot; /&gt;. For breeding heat tolerant rice, it is important that molecular components that underlie the heat shock response in this species are understood &lt;ref name=&quot;ref3&quot; /&gt;. Microarray profiling data have shown that heat stress response in maturing tomato microspores involves heat shock proteins, ROS scavengers, hormones and sugars &lt;ref name=&quot;ref4&quot; /&gt;. Understandably, the major molecular changes in rice plants as affected by heat stress need to be worked out. This is especially relevant since rice has emerged as a model plant species of the group monocots due to its small genome size, availability of large collection of full-length cDNAs (FLcDNAs) and for the fact that the whole genome of this plant species is completely sequenced. This crop has attracted a great deal of efforts for the elucidation of gene functions; completion of genome sequencing in rice has paved way for comprehensive functional characterization of genes, transcription factors, signaling components and promoters &lt;ref name=&quot;ref5&quot; /&gt;. The completed rice genome sequence has been used for the characterization of a large number of gene families involved in diverse processes and pathways. However, with almost 42000 genes, several of them unknown, there are ample proteins still left to be characterized in this important crop species. In recent years, comprehensive details on heat shock regulated rice HSP20, HSP70, HSP90 and HSF gene families have been reported .<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> [1] Peng S, Huang J, Sheehy JE, Laza RC, Visperas RM, Zhong X, Centeno GS, Khush GS, Cassman KG: Rice yields decline with higher night temperature from global warming. Proc Natl Acad Sci 2004, 101(27):9971-9975.<br /> <br /> [2] Grover A, Chandramouli A, Agarwal S, Katiyar-Agarwal S, Agarwal M, Sahi C: Transgenic rice for tolerance against abiotic stresses. Rice Improvement in the Genomic Era Hawarth Press USADutta SK 2009, 237-267.<br /> <br /> [3] Singh A, Grover A: Genetic engineering for heat tolerance in plants. Physiol Mol Biol Plants 2008, 14:155-166.<br /> <br /> [4] Frank G, Pressman E, Ophir R, Althan L, Shaked R, Freedman M, Shen S, Firon N: Transcriptional profiling of maturing tomato (Solanum lycopersicum L.) microspores reveals the involvement of heat shock proteins, ROS scavengers, hormones, and sugars in the heat stress response. Journal of Experimental Botany 2009, 60(13):3891-3908.<br /> <br /> [5] IRGSP: The map-based sequence of the rice genome. Nature 2005, 436(7052):793-800.<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0224700&diff=183445 2014-06-10T08:58:49Z

<p>Maple: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> *It belongs to Heat stress transcription factors (Hsfs),Heat stress transcription factors (Hsfs) are the central regulators of defense response to heat stress.Hsfs diversify in their biological functions [1]. HsfA members are capable of transcriptional activation,while HsfB members act as repressors or as co-activators (e.g., HsfB1) of HsfA members [2]. However,AtHsfA4 activity was reported to be repressed by AtHsf5, which belongs to class A Hsfs. Overexpression of Hsf genes in transgenic plants resulted in an up-regulation of heatstress-associated genes and an enhancement of thermotolerance, whereas the down-regulation of Hsf genes leads to a reduction in the thermotolerance[3][4].<br /> *AtHsfA9 is involved in seed development and controlled by seed-specific transcription factor abscisic acid-insensitive 3 (ABI3).Further studies to elucidate the functions of the other members in the subfamily may also have potential for the development of transgenic plants with improved stress tolerance.<br /> *Temperatures above the normal optimum are sensed as heat stress (HS) by all living organisms. HS disturbs cellular homeostasis and can lead to severe retardation in growth and development, and even death. As sessile organisms, plants are constantly exposed to changes in temperature and other abiotic factors.The accumulation of heat shock proteins (HSPs) under the control of heat stress transcription factors (HSFs) is assumed to play a central role in the heat stress response (HSR) and in acquired thermotolerance in plants and other organisms. Our current understanding of the functions of HSPs and HSFs has been summarized comprehensively in several recent reviews.This indicates that an ABA pathway may be involved in Hsf induction and responsible for control of downstream processes, such as seed development or drought resistance。In this study, eight OsHsfs (OsHsfA1a, OsHsfA2b, OsHsfC1b, OsHsfA3, OsHsfA7, OsHsfA9, OsHsfB2c, and OsHsfC1b) were found to be induced by both heat shock and H2O2 . Heat stress led to the accumulation of H2O2 in tobacco and Arabidopsis culture cells and mustard seedlings.<br /> <br /> ===Expression===<br /> *Expression patterns of OsHsfs:The number of ESTs for a specific gene in a cDNA library is considered to be proportional to the transcript abundance of the mRNA.OsHsfs were expressed at a higher level in panicle and flower than in other tissues.<br /> *The transcription of OsHsfA9 was up-regulated by both HS and H2O2 treatments in a similar manner.<br /> *In Arabidopsis, the heat stress-induced H2O2 is required for effective expression of heat shock genes.<br /> *HSPs were first identified as proteins that are strongly induced by HS. They include Hsp100/ClpB (eukaryotic/ Escherichia coli nomenclature), Hsp90/HtpG, Hsp70/DnaK, Hsp60/GroEL and small HSP (sHSP) proteins.These HSPs are proposed to act as molecular chaperones in protein quality control. The families of HSP genes in plants are complex; because many family members are also essential for normal growth and development, it has been difficult to unravel their roles during stress (and references therein). Other than by extrapolation from their molecular chaperone activity, there is still no specific information on how Hsp70, Hsp90 and Hsp60 contribute to survival of heat stress in plants. With regard to function during heat stress, investigations of Hsp100/ClpB and sHSPs are the most advanced.Genetic analyses in Arabidopsis indicate that Hsp101 interacts with the sHSP chaperone systemto resolubilize protein aggregates after heat stress, a process that requires complex interactions of Hsp101 protein domains.<br /> <br /> ===Evolution===<br /> *Phylogenetic analysis of OsHsfs indicates that the classes A, B and C Hsfs formed three individual clusters. Furthermore, the class A Hsfs were divided into two sub-clusters In a previous study, the N-terminal part and C-terminal part of DBD and HR-A/B regions were used separately to draw phylogenetic trees.Although most proteins fixed their positions in the different phylogenetic trees, a few Hsfs changed their positions.Similar phenomenonwas also observed on the OsHsfs . A more convinced relationship of the Hsfs was revealed by combining the DBD, HR-A/B, and the flexible linker between DBD and HR-A/B.<br /> <br /> ==Labs working on this gene==<br /> *State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, China<br /> *Department of Molecular Cell Biology, JW Goethe University,Biocenter, Max-von-Laue-Strasse,Germany<br /> *Department of Biochemistry and Molecular Biophysics,University of Arizona, USA<br /> <br /> ==References==<br /> *[1]Chuang WANG, Qian ZHANG, Hui-xia SHOU et al. (2009) Identification and expression analysis of OsHsfs in rice.Journal of Zhejiang University SCIENCE B 10(4):291-300.<br /> *[2]Baniwal, S.K., Bharti, K., Chan, K.Y., Fauth, M., Ganguli, A.,Kotak, S., Mishra, S.K., Nover, L., Port, M., Scharf, K.D.,Tripp, J., Weber, C., Zielinski, D., von Koskull-Döring,P., 2004. Heat stress response in plants: a complex game with chaperones and more than twenty heat stress transcription factors. J. Biosci., 29(4):471-487.<br /> *[3]Charng, Y.Y., Liu, H.C., Liu, N.Y., Chi, W.T., Wang, C.N.,Chang, S.H., Wang, T.T., 2007. A heat-inducible transcription factor, HsfA2, is required for extension of acquired thermotolerance in Arabidopsis. Plant Physiol.,143(1):251-262.<br /> *[4]Kotak, S., Larkindale, J., Lee, U., von Koskull-Döring, P.,Vierling, E., Scharf, K.D., 2007a. Complexity of the heat stress response in plants. Curr. Opin. Plant Biol., 10(3):310-316.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0224700|<br /> Description = Similar to HSP protein (Fragment)|<br /> Version = NM_001055964.1 GI:115451656 GeneID:4332113|<br /> Length = 3809 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0224700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:6564909..6568717|<br /> CDS = 6565073..6565396,6566628..6567513,6567990..6568012|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:6564909..6568717<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:6564909..6568717<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgggctctaagaagcggtcgcctcagcatccggccgccgcggcgccccctcccgccgtcggcggcggcggcggcggcgaggtctccggcgatggtggcgcctcgacggcgaacgggccggtggtgccgaagccgtcggaggtggcgccgttcctgacgaaggtgtacgacatggtctcggaccccgcgaccgacaacgttatctcgtgggccgagggcggcggcagcttcgtgatctgggactcgcacgccttcgagcgcgacctccacaggcacttcaagcacagcaatttcaccagcttcatacgccagctcaacacctatggatttcgtaaagttcaccctgatagatgggagtgggccaatgaaggttttattatgggccaaaaacatcttctgaagaccattaagaggaggaagaagtcttctcaggaatcacctagcgagatacagaaggcgcctgtcaaaactgcacctggtactgaaaatattgagataggaaaatatggtggccttgaaaaggaggttgaaacccttaagagggataaagctcttctcatgcagcagcttgtagatctcaggcactaccagcaaacatctaaccttgaagtgcagaatttaattgaacggcttcaagtaatggaacagaaccagcagcagatgatggcacttctagcaatcgttgtccagaatcctagttttctcaaccagcttgtgcagcaacagcagcagcagcgcagatccaactggtggagtcctgatggaagcaagaaaaggagatttcatgctcttgagcagggccctgtaactgatcaggagacctctggccgtggggcacatattgttgaatatctcccacctgtcccagaaacatcaggtcaagtaaatccagtggaaggagccatttgttcggccaactcacaaccagttccaagtcctgcagttgccacacccatggacatgcaaacgtctaacgttgctgatactctcggttcatctgaggagcctttcgctgataactctactctacatgaatgggatgataacgacatgcagcttctgtttgatgataacctagacccaatacttccaccatttgagaatgatggtcaaatgggccctcctttgagtgttcaagattatgattttccgcagttagagcaggattgtctgatggaagcacaatataactccaacaatcctcaatatgctgatgtcattaccgaagcttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MGSKKRSPQHPAAAAPPPAVGGGGGGEVSGDGGASTANGPVVPK PSEVAPFLTKVYDMVSDPATDNVISWAEGGGSFVIWDSHAFERDLHRHFKHSNFTSFI RQLNTYGFRKVHPDRWEWANEGFIMGQKHLLKTIKRRKKSSQESPSEIQKAPVKTAPG TENIEIGKYGGLEKEVETLKRDKALLMQQLVDLRHYQQTSNLEVQNLIERLQVMEQNQ QQMMALLAIVVQNPSFLNQLVQQQQQQRRSNWWSPDGSKKRRFHALEQGPVTDQETSG RGAHIVEYLPPVPETSGQVNPVEGAICSANSQPVPSPAVATPMDMQTSNVADTLGSSE EPFADNSTLHEWDDNDMQLLFDDNLDPILPPFENDGQMGPPLSVQDYDFPQLEQDCLM EAQYNSNNPQYADVITEA&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;165..488#1720..2605#3082..3104#ctagtacaccagacgacaacacacacacaaaaaaaaaaaagaagagaaaaaccgaacgaaaatcccctcgcgcagacgactactacatccttctcgtctcatcccgtcgacccgccgccgcctcaatctcgaagcctcggcgaggctagggtttctgcgtctccatgggctctaagaagcggtcgcctcagcatccggccgccgcggcgccccctcccgccgtcggcggcggcggcggcggcgaggtctccggcgatggtggcgcctcgacggcgaacgggccggtggtgccgaagccgtcggaggtggcgccgttcctgacgaaggtgtacgacatggtctcggaccccgcgaccgacaacgttatctcgtgggccgagggcggcggcagcttcgtgatctgggactcgcacgccttcgagcgcgacctccacaggcacttcaagcacagcaatttcaccagcttcatacgccagctcaacacctatgtgagattcccttgatccctgtgttcgtttgtttctgatgcgcgaattcggtgccatttccagttggttggacgaaatcggtagtgaaatctgagtttctgttcccaatttgttgtttattatcacggctagacagcaaatcttgcttgcaattataaattgggcagtagcttccgatgcactgcatatgaatttgtttgaatcgtgactgcgtgttatatgatgttactttcttctattttgtgccttttaagttgattgcgtgtattagggattatctgccattttggagttaatcacttattaaattttaactacgttggaagctgttggcgatatagttggatgcctttcttcttctagtcgaaagacacaatagaactagtttctgcggtggctttctattcaacaacaacttctagagttctagtacataaaacacgtcattgggggtggggactaatattgttaattttgagaccagtttctgttctcttaatgttttgtgaatttgatcgtatgaaagctggctgtagaaactatgccaaactcttggctttaaattacaggggaatgatatgtgttgcctatcactacttctaaaagtctatttcccccatatttgtatttagtgaacttcagaactagagaaagcatgatgttataacctaccatcatgctgtataagaagaatatgttccctcttgtttggctgaaagaaatgcatgtttggttgtgctgatattgtactgctattatcttcttctgtgtcatttgcctcaatacattgttttatttgaatttgaacttccttttcacaatcgaatttgtctgatgaaatataagattatgaatttgctcttttattgaactatatggacacttaattttgtgcttttggcaaatccatgtcctcattttgatttctggataaatcactggactcttgcacccaggatttcttctgatgtctaattacttaaatgcgaaaatcacctgacttttgctcactgggttgctttcctgtagttaggagtagttctatttaaccatctattagttgttcttggcttcatatactcagtaaaacttaaaaacaaagcctgaaaagtgaaatctccaatgggatcagtagaaacattactttatctgctttcattgatgttctctattcttttggagcatatgttttatattagctgaagttactcaagtttcctttcataatcagggatttcgtaaagttcaccctgatagatgggagtgggccaatgaaggttttattatgggccaaaaacatcttctgaagaccattaagaggaggaagaagtcttctcaggaatcacctagcgagatacagaaggcgcctgtcaaaactgcacctggtactgaaaatattgagataggaaaatatggtggccttgaaaaggaggttgaaacccttaagagggataaagctcttctcatgcagcagcttgtagatctcaggcactaccagcaaacatctaaccttgaagtgcagaatttaattgaacggcttcaagtaatggaacagaaccagcagcagatgatggcacttctagcaatcgttgtccagaatcctagttttctcaaccagcttgtgcagcaacagcagcagcagcgcagatccaactggtggagtcctgatggaagcaagaaaaggagatttcatgctcttgagcagggccctgtaactgatcaggagacctctggccgtggggcacatattgttgaatatctcccacctgtcccagaaacatcaggtcaagtaaatccagtggaaggagccatttgttcggccaactcacaaccagttccaagtcctgcagttgccacacccatggacatgcaaacgtctaacgttgctgatactctcggttcatctgaggagcctttcgctgataactctactctacatgaatgggatgataacgacatgcagcttctgtttgatgataacctagacccaatacttccaccatttgagaatgatggtcaaatgggccctcctttgagtgttcaagattatgattttccgcagttagagcaggattgtctgatggaagcacaatataactccaacaatcctcaatatggtaatgactaattactgtataccctatgtttttgttcatcattttgtggaaaacaagcgtgatgtcattttagttaactgtggagtttctatgtgcatgtttcatgccaaatgccaaacaatggatgtttacagaccacccttttagtctgttgtgctaggatttggcaaagatgacatggagccttattcaaagttcaatacttgcttctcaaatggctttgatatttccttctgttatttcacgaaagtagtgtttgacaactagagtgatatttttttttatatctggttaattggacaattttgatcacagccaaacctcttgatttagtttcctcaaccaaaacagatctaagctgtgcctgcgtcacattgattattttcccaatcagcctattctttgtttgatcatatcggtttcaattcactctgcagtacttctatacatgcctgacatcttccctttttcatcagctgatgtcattaccgaagcttgattggtggtccaggagcatacttgcagtaacattaggtgggtacagtccatttttaacatgaaaaaggaattattccttaatgtgctttgttaatggaatgcttactttgcgttgcaataccaaagtaccaaactgaaagacataccatccttgtttaattcctctagccgttgtttcttacagaactttaggctagtatttcgtgtcaggctaggactatatggtcaactaaccctgcaaccgcctccaaattgaaattcaatcgaaaaaatattgaacttaatggcttgaactgaatgcttactaaataaacatgattggtcgttaaaaaaatgccatcagccatttcttgtaaataaatacacgaagctatattttttttatttgtatgaccaatgtttgttccagtactgatccagtttcaacatctgcagttcttcggtgaccaacctccgggcgcatctgcaccattggcatcccgaaatggcgtcctcagggtgtaccgatgaattatgctaacacagaagaagatataactctaatctggttgaccatacagaactacctttcttgttcaaggcactttttggggacaggggacgccttttcgcagttctcgtcgtcttacactagcgatggttgctaccatcgaattcatttggtgtatatactgttgttcgtcttataaaacatgagcagttttgt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055964.1 RefSeq:Os03g0224700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=183442 2014-06-10T08:54:21Z

<p>Maple: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues.<br /> <br /> Heat stress threatens future prospects of increased grain production in crops. Rice (Oryza sativa) is most important world food crop. The production of rice is getting severely affected with increases in mean global temperature. According to estimates, yield of rice declines by 10% for every 1°C increase in growing period minimum temperature in the dry season &lt;ref name=&quot;ref1&quot; /&gt;. Processes like spikelet fertility, grain quality and yield processes in rice are considered to be especially sensitive to heat stress &lt;ref name=&quot;ref2&quot; /&gt;. For breeding heat tolerant rice, it is important that molecular components that underlie the heat shock response in this species are understood &lt;ref name=&quot;ref3&quot; /&gt;. Microarray profiling data have shown that heat stress response in maturing tomato microspores involves heat shock proteins, ROS scavengers, hormones and sugars &lt;ref name=&quot;ref4&quot; /&gt;. Understandably, the major molecular changes in rice plants as affected by heat stress need to be worked out. This is especially relevant since rice has emerged as a model plant species of the group monocots due to its small genome size, availability of large collection of full-length cDNAs (FLcDNAs) and for the fact that the whole genome of this plant species is completely sequenced. This crop has attracted a great deal of efforts for the elucidation of gene functions; completion of genome sequencing in rice has paved way for comprehensive functional characterization of genes, transcription factors, signaling components and promoters &lt;ref name=&quot;ref5&quot; /&gt;. The completed rice genome sequence has been used for the characterization of a large number of gene families involved in diverse processes and pathways. However, with almost 42000 genes, several of them unknown, there are ample proteins still left to be characterized in this important crop species. In recent years, comprehensive details on heat shock regulated rice HSP20, HSP70, HSP90 and HSF gene families have been reported .<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> [1] Peng S, Huang J, Sheehy JE, Laza RC, Visperas RM, Zhong X, Centeno GS, Khush GS, Cassman KG: Rice yields decline with higher night temperature from global warming. Proc Natl Acad Sci 2004, 101(27):9971-9975.<br /> <br /> [2] Grover A, Chandramouli A, Agarwal S, Katiyar-Agarwal S, Agarwal M, Sahi C: Transgenic rice for tolerance against abiotic stresses. Rice Improvement in the Genomic Era Hawarth Press USADutta SK 2009, 237-267.<br /> <br /> [3] Singh A, Grover A: Genetic engineering for heat tolerance in plants. Physiol Mol Biol Plants 2008, 14:155-166.<br /> <br /> [4] Frank G, Pressman E, Ophir R, Althan L, Shaked R, Freedman M, Shen S, Firon N: Transcriptional profiling of maturing tomato (Solanum lycopersicum L.) microspores reveals the involvement of heat shock proteins, ROS scavengers, hormones, and sugars in the heat stress response. Journal of Experimental Botany 2009, 60(13):3891-3908.<br /> <br /> [5] IRGSP: The map-based sequence of the rice genome. Nature 2005, 436(7052):793-800.<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=183440 2014-06-10T08:53:37Z

<p>Maple: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues.<br /> <br /> Heat stress threatens future prospects of increased grain production in crops. Rice (Oryza sativa) is most important world food crop. The production of rice is getting severely affected with increases in mean global temperature. According to estimates, yield of rice declines by 10% for every 1°C increase in growing period minimum temperature in the dry season &lt;ref name=&quot;ref1&quot; /&gt;. Processes like spikelet fertility, grain quality and yield processes in rice are considered to be especially sensitive to heat stress &lt;ref name=&quot;ref2&quot; /&gt;. For breeding heat tolerant rice, it is important that molecular components that underlie the heat shock response in this species are understood &lt;ref name=&quot;ref3&quot; /&gt;. Microarray profiling data have shown that heat stress response in maturing tomato microspores involves heat shock proteins, ROS scavengers, hormones and sugars &lt;ref name=&quot;ref4&quot; /&gt;. Understandably, the major molecular changes in rice plants as affected by heat stress need to be worked out. This is especially relevant since rice has emerged as a model plant species of the group monocots due to its small genome size, availability of large collection of full-length cDNAs (FLcDNAs) and for the fact that the whole genome of this plant species is completely sequenced. This crop has attracted a great deal of efforts for the elucidation of gene functions; completion of genome sequencing in rice has paved way for comprehensive functional characterization of genes, transcription factors, signaling components and promoters &lt;ref name=&quot;ref5&quot; /&gt;. The completed rice genome sequence has been used for the characterization of a large number of gene families involved in diverse processes and pathways. However, with almost 42000 genes, several of them unknown, there are ample proteins still left to be characterized in this important crop species. In recent years, comprehensive details on heat shock regulated rice HSP20, HSP70, HSP90 and HSF gene families have been reported .<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> [1] Peng S, Huang J, Sheehy JE, Laza RC, Visperas RM, Zhong X, Centeno GS, Khush GS, Cassman KG: Rice yields decline with higher night temperature from global warming. Proc Natl Acad Sci 2004, 101(27):9971-9975.<br /> [2] Grover A, Chandramouli A, Agarwal S, Katiyar-Agarwal S, Agarwal M, Sahi C: Transgenic rice for tolerance against abiotic stresses. Rice Improvement in the Genomic Era Hawarth Press USADutta SK 2009, 237-267.<br /> [3] Singh A, Grover A: Genetic engineering for heat tolerance in plants. Physiol Mol Biol Plants 2008, 14:155-166.<br /> [4] Frank G, Pressman E, Ophir R, Althan L, Shaked R, Freedman M, Shen S, Firon N: Transcriptional profiling of maturing tomato (Solanum lycopersicum L.) microspores reveals the involvement of heat shock proteins, ROS scavengers, hormones, and sugars in the heat stress response. Journal of Experimental Botany 2009, 60(13):3891-3908.<br /> [5] IRGSP: The map-based sequence of the rice genome. Nature 2005, 436(7052):793-800.<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=183436 2014-06-10T08:50:21Z

<p>Maple: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues.<br /> <br /> Heat stress threatens future prospects of increased grain production in crops. Rice (Oryza sativa) is most important world food crop. The production of rice is getting severely affected with increases in mean global temperature. According to estimates, yield of rice declines by 10% for every 1°C increase in growing period minimum temperature in the dry season &lt;ref name=&quot;ref1&quot; /&gt;. Processes like spikelet fertility, grain quality and yield processes in rice are considered to be especially sensitive to heat stress &lt;ref name=&quot;ref2&quot; /&gt;. For breeding heat tolerant rice, it is important that molecular components that underlie the heat shock response in this species are understood &lt;ref name=&quot;ref3&quot; /&gt;. Microarray profiling data have shown that heat stress response in maturing tomato microspores involves heat shock proteins, ROS scavengers, hormones and sugars &lt;ref name=&quot;ref4&quot; /&gt;. Understandably, the major molecular changes in rice plants as affected by heat stress need to be worked out. This is especially relevant since rice has emerged as a model plant species of the group monocots due to its small genome size, availability of large collection of full-length cDNAs (FLcDNAs) and for the fact that the whole genome of this plant species is completely sequenced. This crop has attracted a great deal of efforts for the elucidation of gene functions; completion of genome sequencing in rice has paved way for comprehensive functional characterization of genes, transcription factors, signaling components and promoters &lt;ref name=&quot;ref5&quot; /&gt;. The completed rice genome sequence has been used for the characterization of a large number of gene families involved in diverse processes and pathways. However, with almost 42000 genes, several of them unknown, there are ample proteins still left to be characterized in this important crop species. In recent years, comprehensive details on heat shock regulated rice HSP20, HSP70, HSP90 and HSF gene families have been reported .<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> [1]Peng S, Huang J, Sheehy JE, Laza RC, Visperas RM, Zhong X, Centeno GS, Khush GS, Cassman KG: Rice yields decline with higher night temperature from global warming. Proc Natl Acad Sci 2004, 101(27):9971-9975.<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=183435 2014-06-10T08:49:41Z

<p>Maple: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues.<br /> <br /> Heat stress threatens future prospects of increased grain production in crops. Rice (Oryza sativa) is most important world food crop. The production of rice is getting severely affected with increases in mean global temperature. According to estimates, yield of rice declines by 10% for every 1°C increase in growing period minimum temperature in the dry season &lt;ref name=&quot;ref1&quot; /&gt;. Processes like spikelet fertility, grain quality and yield processes in rice are considered to be especially sensitive to heat stress &lt;ref name=&quot;ref2&quot; /&gt;. For breeding heat tolerant rice, it is important that molecular components that underlie the heat shock response in this species are understood &lt;ref name=&quot;ref3&quot; /&gt;. Microarray profiling data have shown that heat stress response in maturing tomato microspores involves heat shock proteins, ROS scavengers, hormones and sugars &lt;ref name=&quot;ref4&quot; /&gt;. Understandably, the major molecular changes in rice plants as affected by heat stress need to be worked out. This is especially relevant since rice has emerged as a model plant species of the group monocots due to its small genome size, availability of large collection of full-length cDNAs (FLcDNAs) and for the fact that the whole genome of this plant species is completely sequenced. This crop has attracted a great deal of efforts for the elucidation of gene functions; completion of genome sequencing in rice has paved way for comprehensive functional characterization of genes, transcription factors, signaling components and promoters &lt;ref name=&quot;ref5&quot; /&gt;. The completed rice genome sequence has been used for the characterization of a large number of gene families involved in diverse processes and pathways. However, with almost 42000 genes, several of them unknown, there are ample proteins still left to be characterized in this important crop species. In recent years, comprehensive details on heat shock regulated rice HSP20, HSP70, HSP90 and HSF gene families have been reported .<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> &lt;ref name=&quot;ref1&quot;&gt;Peng S, Huang J, Sheehy JE, Laza RC, Visperas RM, Zhong X, Centeno GS, Khush GS, Cassman KG: Rice yields decline with higher night temperature from global warming. Proc Natl Acad Sci 2004, 101(27):9971-9975.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=182908 2014-06-09T15:57:12Z

<p>Maple: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues&lt;ref name=&quot;ref1&quot; /&gt;<br /> .<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> &lt;ref name=&quot;ref1&quot;&gt; Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987, 162(1):156-159.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=182903 2014-06-09T15:56:32Z

<p>Maple: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues&lt;ref name=&quot;ref1&quot; /&gt;<br /> .<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> &lt;ref name=&quot;ref1&quot;&gt;Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987, 162(1):156-159.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=182900 2014-06-09T15:55:45Z

<p>Maple: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues&lt;ref name=&quot;ref1&quot; /&gt;<br /> .<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987, 162(1):156-159.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=182897 2014-06-09T15:55:09Z

<p>Maple: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues&lt;ref name=&quot;ref1&quot; /&gt;<br /> .<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> Please input cited references here.<br /> &lt;ref name=&quot;ref1&quot;&gt;Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid<br /> guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem<br /> 1987, 162(1):156-159.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=182894 2014-06-09T15:54:06Z

<p>Maple: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues&lt;ref name=&quot;ref1&quot; /&gt;<br /> .<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> Please input cited references here.<br /> &lt;ref name=&quot;ref1&quot;&gt; Takahashi T, Naito S, Komeda Y (1992) Isolation and analysis of the expression of two genes for the 81-kilodalton heat-shock proteins from Arabidopsis. Plant Physiol 99:383–390.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=182892 2014-06-09T15:53:39Z

<p>Maple: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues&lt;ref name=&quot;ref1&quot; /&gt;<br /> .<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> Please input cited references here.<br /> &lt;ref name=&quot;ref1&quot;&gt; Takahashi T, Naito S, Komeda Y (1992) Isolation and analysis of the<br /> expression of two genes for the 81-kilodalton heat-shock proteins<br /> from Arabidopsis. Plant Physiol 99:383–390.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=182887 2014-06-09T15:52:12Z

<p>Maple: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues&lt;ref name=&quot;ref1&quot; /&gt;<br /> .<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> Please input cited references here.<br /> &lt;ref name=&quot;ref1&quot;&gt; Amanjot Singh, Dheeraj Mittal: OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB (Hsp100) gene in rice. Cell Stress and Chaperones, 2012, 17(2): 243-254.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=182883 2014-06-09T15:50:30Z

<p>Maple: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues&lt;ref name=&quot;ref1&quot; /&gt;<br /> .<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> *Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> Please input cited references here.<br /> &lt;ref name=&quot;ref1&quot;&gt; Amanjot Singh, Dheeraj Mittal: OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB (Hsp100) gene in rice (Oryza sativa L.)Cell Stress and Chaperones, 2012, 17(2): 243-254.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=182877 2014-06-09T15:48:23Z

<p>Maple: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues&lt;ref name=&quot;ref1&quot; /&gt;<br /> .<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> Department of Plant Molecular Biology,University of Delhi South Campus,New Delhi 110021, India<br /> <br /> ==References==<br /> Please input cited references here.<br /> &lt;ref name=&quot;ref1&quot;&gt; Amanjot Singh, Dheeraj Mittal: OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB (Hsp100) gene in rice (Oryza sativa L.)Cell Stress and Chaperones, 2012, 17(2): 243-254.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=182874 2014-06-09T15:46:47Z

<p>Maple: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues&lt;ref name=&quot;ref1&quot; /&gt;<br /> .<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> &lt;ref name=&quot;ref1&quot;&gt; Amanjot Singh, Dheeraj Mittal: OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB (Hsp100) gene in rice (Oryza sativa L.)Cell Stress and Chaperones, 2012, 17(2): 243-254.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=182871 2014-06-09T15:46:23Z

<p>Maple: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues&lt;ref name=&quot;ref1&quot; /&gt;<br /> .<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> &lt;ref name=&quot;ref1&quot;&gt; Amanjot Singh, Dheeraj Mittal, et, al. OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB (Hsp100) gene in rice (Oryza sativa L.)Cell Stress and Chaperones, 2012, 17(2): 243-254.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=182857 2014-06-09T15:40:38Z

<p>Maple: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues&lt;ref name=&quot;ref1&quot; /&gt;<br /> .<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> &lt;ref name=&quot;ref1&quot;&gt; OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB (Hsp100) gene in rice (Oryza sativa L.)Cell Stress and Chaperones, 2012, 17(2): 243-254.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=182849 2014-06-09T15:37:10Z

<p>Maple: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues&lt;ref name=&quot;ref1&quot; /&gt;<br /> .<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0703900&diff=182799 2014-06-09T15:15:24Z

<p>Maple: /* Expression */</p> <hr /> <div>Gene Os11g0703900, namely '''Hsp70''',meaning rice heat shock protein gene,is a multigenic family, andregulates the expression of stress response genes, such as the regulation of the heat shock protein transcription factor (heat-shock factor, HSF).<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> *Heat shock proteins (heat shock protein, Hsp) are a class of resistance protein molecules playing an important role in the process of environmental stress&lt;ref name=&quot;ref1&quot; /&gt;&lt;ref name=&quot;ref2&quot; /&gt;&lt;ref name=&quot;ref3&quot; /&gt;.<br /> <br /> *Hsp70 genes encode for a group of highly conserved chaperone proteins across the living systems encompassing bacteria, plants, and animals&lt;ref name=&quot;ref4&quot; /&gt;.<br /> <br /> *Hsp70 can not only prevent proteins aggregation and help inactivated proteins refolding, but also assist the functions of transport and participate in the intracellular signal transduction, such as protein kinase C (protein kinase C, PKC) and protein phosphorylation activity (protein phosphatase)&lt;ref name=&quot;ref5&quot; /&gt;.<br /> <br /> *It is noted that Hsp70 may function as a negative feedback regulator of HSF activity&lt;ref name=&quot;ref6&quot; /&gt;&lt;ref name=&quot;ref7&quot; /&gt;.In this process, Hsp70 binds to denatured proteins and releases HSF leading to its activation during heat stress&lt;ref name=&quot;ref6&quot; /&gt;.<br /> <br /> *Heat stress results in misfolding and aggregation of cellular proteins. Heat shock proteins (Hsp) enable the cells to maintain proper folding of proteins, both in unstressed as well as stressed conditions. Hsp70 genes encode for a group of highly conserved chaperone proteins across the living systems encompassing bacteria, plants, and animals. In the cellular chaperone network, Hsp70 family proteins interconnect other chaperones and play a dominant role in various cell processes.<br /> <br /> ===Expression===<br /> <br /> *Rice Hsp70 superfamily genes are represented by 24 Hsp70 family and 8 Hsp110 family members&lt;ref name=&quot;ref4&quot; /&gt;. <br /> <br /> *In rice, ER Hsp70 (BiP) is found to associate with nascent polypeptides that emerge into the ER lumen during prolamin translocation and facilitate peptide folding and assembly&lt;ref name=&quot;ref9&quot; /&gt;. Over-expression of BiP relieves &quot;ER stress&quot;&lt;ref name=&quot;ref10&quot; /&gt;.<br /> <br /> *Rice mitochondrial Hsp70 has been proposed as suppressor of heat and H2O2-induced programmed cell death&lt;ref name=&quot;ref11&quot; /&gt;. <br /> <br /> [[File:Fig 1.Over-expression of mtHSP70 suppressed heat-induced PCD in rice protoplasts.jpg|right|thumb|360px|Fig 1.Over-expression of mtHSP70 suppressed heat-induced PCD in rice protoplasts..&lt;ref name=&quot;ref11&quot; /&gt;'']]<br /> <br /> [[File:Fig.2. Over-expression of mtHSP70 suppressed H2O2-induced PCD in rice protoplasts.jpg|right|thumb|360px|Fig.2. Over-expression of mtHSP70 suppressed H2O2-induced PCD in rice protoplasts..&lt;ref name=&quot;ref11&quot; /&gt;'']]<br /> <br /> *Hsp70 regulates the expression of stress response genes, such as the regulation of the heat shock protein transcription factor (heat-shock factor, HSF)&lt;ref name=&quot;ref8&quot; /&gt;&lt;ref name=&quot;ref12&quot; /&gt;.<br /> <br /> *Overexpression of Hsp70 will decrease the concentration of Ca2+ intracellular&lt;ref name=&quot;ref13&quot; /&gt;, which usually works as a second messenger to stimulate the activity of protein kinases in downstream when the organism faces an environmental stress&lt;ref name=&quot;ref14&quot; /&gt;.<br /> <br /> *Hsp70 are ATP-dependent chaperones having a conserved ~44-kD N-terminal ATPase domain (also called nucleotide binding domain; NBD), a ~18-kD substrate binding domain (SBD) and a ~10-kD variable C-terminal “lid.”.<br /> <br /> *Hsp70 proteins require two cochaperones in the form of J-domain proteins and nucleotide exchange factors (NEFs) for their functions. Binding of J-domain proteins stimulates ATP hydrolysis that facilitates trapping of exposed hydrophobic region of substrates and closed conformation of SBD. NEFs participate in ATP–ADP exchange by catalyzing the release of ADP from Hsp70, leading to conversion of open conformation Hsp70 and substrate release&lt;ref name=&quot;ref4&quot; /&gt;.<br /> <br /> *The stress induced expression profile of Hsp70 superfamily genes showed diverse response.<br /> <br /> ===Evolution===<br /> <br /> <br /> *Hsp70 is a multigenic family. Various members constituting this family are present in different cellular compartments. In Saccharomyces cerevisiae, 14 Hsp70 genes representing nine cytosolic, three mitochondrial (Ssc, Ecm, and Ssq), and two ER (Kar2 and Lhs1) isoforms have been identified&lt;ref name=&quot;ref15&quot; /&gt;.In E. coli, there are three Hsp70 genes. Arabidopsis has 18 Hsp70 superfamily members, of which 14 belong to Hsp70 family and 4 belong to Hsp110/SSE family&lt;ref name=&quot;ref16&quot; /&gt;.The C-terminus of organellar Hsp70 proteins is highly conserved and unique for each organelle. The motif for the cytosolic group is EEVD, for the mitochondrion is PEAEYEEAKK and for the plastid is PEGDVIDADFTDSK &lt;ref name=&quot;ref17&quot; /&gt;.The C terminus EEVD motif of cytosolic Hsp70 proteins interacts with co-chaperones that contain several degenerate 34 amino acid repeats, called tetratricopeptide repeats.<br /> <br /> *While Hsp70 genes clustered on clades A, B, and C, clade D contained Hsp110 family genes. Hsp110 family is a subfamily of Hsp70 superfamily, structurally very similar to Hsp70 having N-terminal ATPase domain and C-terminal peptide binding domain and functions as NEFs for Hsp70 family proteins&lt;ref name=&quot;ref5&quot; /&gt;.<br /> <br /> *During heat stress the expression of hsp70 correlates well with increases in internal ion concentrations, and can also be induced by excess salt or ethanol at normal growth temperatures. These results appear to indicate that whereas hsp70 is induced by all stresses that lead to protein denaturation — including heat stress&lt;ref name=&quot;ref18&quot; /&gt;.<br /> <br /> ==Labs working on this gene==<br /> <br /> <br /> *1.Sarkar N K, Kundnani P, Grover A.Department of Plant Molecular Biology, University of Delhi South Campus, N Delhi 110021, India<br /> <br /> *2.Wei ZhangCollege of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China<br /> <br /> *3.Laboratorium voor Genetica, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium<br /> <br /> *4.An Department of Plant Molecular Systems Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 446-701, South Korea<br /> <br /> *5.Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Ahuja I, RCH. De Vos, Bones AM, Hall RD: Plant molecular stress responses face climate change. Trends in Plant Science 2010, Vol. 15, No. 12.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref2&quot;&gt;Wang W, Vinocur B, Shoseyov O, Altman A: Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends in Plant Science 2004, Vol. 9, No. 5. &lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref3&quot;&gt;Timperio AM, Egidi MG, Zolla L: Proteomics applied on plant abiotic stresses: Role of heat shock proteins (HSP). Journal of Proteomics 2008, 71: 391-411.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref4&quot;&gt;Sarkar N K, Kundnani P, Grover A. Functional analysis of Hsp70 superfamily proteins of rice (Oryza sativa)[J]. Cell Stress and Chaperones, 2013, 18(4): 427-437.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref5&quot;&gt; Ding XZ, Tsokos GC, Kiang JG: Overexpression of HSP-70 inhibits the phosphorylation of HSF1 by activating protein phosphatase and inhibiting protein kinase C activity. The FASEB Journal 1998, 12:451–459.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref6&quot;&gt;Shi Y, Mosser D D, Morimoto R I. Molecularchaperones as HSF1-specific transcriptional repressors[J]. Genes &amp; development, 1998, 12(5): 654-666.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref7&quot;&gt;Kim B H, Schöffl F. Interaction between Arabidopsis heat shock transcription factor 1 and 70 kDa heat shock proteins[J]. Journal of experimental botany, 2002, 53(367): 371-375.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref8&quot;&gt;Sarkar N K, Kundnani P, Grover A. Functional analysis of Hsp70 superfamily proteins of rice (Oryza sativa)[J]. Cell Stress and Chaperones, 2013, 18(4): 427-437.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref9&quot;&gt;Muench D G, Wu Y, Zhang Y, et al. Molecular cloning, expression and subcellular localization of a BiP homolog from rice endosperm tissue[J]. Plant and cell physiology, 1997, 38(4): 404-412.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref10&quot;&gt;Snowden C J, Leborgne‐Castel N, Wootton L J, et al. In vivo analysis of the lumenal binding protein (BiP) reveals multiple functions of its ATPase domain[J]. The Plant Journal, 2007, 52(6): 987-1000. &lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref11&quot;&gt;Qi Y, Wang H, Zou Y, et al. Over-expression of mitochondrial heat shock protein 70 suppresses programmed cell death in rice[J]. FEBS letters, 2011, 585(1): 231-239.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref12&quot;&gt; Kim BH, Schöffl F. Interaction between Arabidopsis heat shock transcription factor 1 and 70 kDa heat shock proteins. Journal of Experimental Botany 2002, 53(367):371-375.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref13&quot;&gt; Kiang JG, Ding XZ, McClain DE. Overexpression of HSP-70 attenuates increases in [Ca2+]I and protects human epidermoid A-431 cells after chemical hypoxia. Toxicology and Applied Pharmacology 1998, 149(2):185-194.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref14&quot;&gt;Knight H: Calcium signaling during abiotic stress in plants. International Review of Cytology 2000, 195:269-324.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref15&quot;&gt;Walsh P, Bursać D, Law Y C, et al. The J‐protein family: modulating protein assembly, disassembly and translocation[J]. EMBO reports, 2004, 5(6): 567-571.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref16&quot;&gt; Lin B L, Wang J S, Liu H C, et al. Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana[J]. Cell stress &amp; chaperones, 2001, 6(3): 201.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref17&quot;&gt; Guy C L, Li Q B. The organization and evolution of the spinach stress 70 molecular chaperone gene family[J]. The Plant Cell Online, 1998, 10(4): 539-556.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref18&quot;&gt;Frank Van Breusegem, Rudy Dekeyser, Ana Beatriz Garcia,et al.Heat-inducible rice hsp82 and hsp70 are not always co-regulated[J].Planta, 1994, 193(1): 57-66.&lt;/ref&gt; <br /> &lt;references&gt;<br /> <br /> ==Structured Information==<br /> *Hsp70 are ATP-dependent chaperones having a conserved ~44-kD N-terminal ATPase domain (also called nucleotide binding domain; NBD), a ~18-kD substrate binding<br /> domain (SBD) and a ~10-kD variable C-terminal “lid.” The flexible C-terminal lid assists in holding the substrates at SBD. ATPase domain performs regulatory role and SBD associates to hydrophobic regions exposed in non-native substrates.<br /> {{JaponicaGene|<br /> GeneName = Os11g0703900|<br /> Description = Heat shock protein 70|<br /> Version = NM_001075072.1 GI:115486792 GeneID:4351208|<br /> Length = 4293 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0703900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:30612818..30617110|<br /> CDS = 30612889..30613102,30615017..30616752|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:30612818..30617110<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:30612818..30617110<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcgggcaagggcgagggtccggccatcggcatcgaccttggcacgacctactcgtgcgtgggcgtttggcagcacgaccgcgtggagatcatcgccaacgaccagggcaaccgcaccaccccctcctacgtcggcttcaccgactccgagaggctcatcggagatgctgccaagaaccaggtcgccatgaaccccatcaacaccgtctttgatgccaagcgtctcattggcaggaggtttagcgatgcttctgttcagagtgacattaagctctggcccttcaaggtgattgctggacctggtgacaagcctatgattgttgtccagtacaagggtgaggagaagcagtttgctgcagaagagatctcctccatggtcctcatcaagatgcgtgagattgctgaggcctaccttggcaccaccatcaagaatgccgttgtcactgttcctgcctacttcaatgactcccagaggcaggccaccaaggatgctggagtgattgctggtctcaatgtcatgcgtatcatcaacgagccgactgctgcagctattgcctatggtcttgataagaaggccaccagcgttggtgagaagaatgtcctcatctttgaccttggaggtggtacctttgatgtctccctccttaccattgaggagggtatctttgaggtcaaggccacagctggtgacacccatcttggtggtgaagattttgacaatcgtatggtcaaccactttgtgcaagaattcaagaggaagaacaagaaggatatcactggcaaccccagggctctcaggaggttgaggacagcttgtgagagggcaaagaggaccctgtcctccactgcccagaccaccattgagatcgattccctgtatgagggcatcgacttctactcaaccatcacccgtgccaggtttgaggagctcaacatggatctcttcaggaagtgtatggagcctgtggagaagtgcctcagggatgctaagatggacaagagctctgttcatgatgttgtccttgttggtggctccactaggatcccgagggtgcagcagctcctgcaggatttcttcaacggcaaggagctttgcaagaacatcaacccagatgaggctgttgcttatggtgctgctgtccaggctgccatcttgagtggtgagggcaacgagaaggtccaggacctcctcctgttggatgttacccctctctctctcggtttggagactgctggtggtgtcatgaccgttttgatcccaaggaacaccaccattcccaccaagaaggagcaggtcttctccacctactccgacaaccagcctggtgtgctcatccaggtttatgagggtgagaggaccaggacacgtgacaacaacctgctggggaagtttgagctctctggaatccctcctgctcccaggggtgttccacagatcactgtttgcttcgacattgatgccaatggtatcctgaacgtgtctgctgaggacaagaccaccgggcagaagaacaagatcaccattaccaacgacaagggcaggcttagcaaggaggagattgagaagatggtccaggaggccgagaagtacaagtcagaggatgaggagcacaagaagaaggtggagtccaagaacgcgctggagaactacgcctacaacatgcgcaacaccatcaaggatgagaagatcgcctcgaagctcccggcagcggacaagaagaagatcgaggatgccatcgaccaggccatccagtggctggacggcaaccagctcgctgaggctgatgagttcgatgacaagatgaaggagctggagggcatctgcaaccccatcatcgccaagatgtaccagggcgctggcgcggacatggccggcggcatggacgaggacgatgctcccccggctggcggcagcggtgctggccccaagatcgaggaggtcgactaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSY VGFTDSERLIGDAAKNQVAMNPINTVFDAKRLIGRRFSDASVQSDIKLWPFKVIAGPG DKPMIVVQYKGEEKQFAAEEISSMVLIKMREIAEAYLGTTIKNAVVTVPAYFNDSQRQ ATKDAGVIAGLNVMRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTI EEGIFEVKATAGDTHLGGEDFDNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERA KRTLSSTAQTTIEIDSLYEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDK SSVHDVVLVGGSTRIPRVQQLLQDFFNGKELCKNINPDEAVAYGAAVQAAILSGEGNE KVQDLLLLDVTPLSLGLETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYE GERTRTRDNNLLGKFELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKIT ITNDKGRLSKEEIEKMVQEAEKYKSEDEEHKKKVESKNALENYAYNMRNTIKDEKIAS KLPAADKKKIEDAIDQAIQWLDGNQLAEADEFDDKMKELEGICNPIIAKMYQGAGADM AGGMDEDDAPPAGGSGAGPKIEEVD&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;72..285#2200..3935#ctctcccactcttcctcctccccaaaccctagccgccgccgccggcgtactcgagagaaacatcagcatccatggcgggcaagggcgagggtccggccatcggcatcgaccttggcacgacctactcgtgcgtgggcgtttggcagcacgaccgcgtggagatcatcgccaacgaccagggcaaccgcaccaccccctcctacgtcggcttcaccgactccgagaggctcatcggagatgctgccaagaaccaggtcgccatgaaccccatcaacaccgtctttggtaactacttattccctgctcctcagtcctctctctttctctctctccccccttagatctcatctctaaggtcttcgtcgtcgtcgtatgctagatctactcttgcccagctcgttcagatctgttagatttgtgtgggatgctctaattaattactaccaacttagtagatctggaactcttttgacttgcagatcttgctccactcctgttgcattttgaatctgtgttgatgtgatttaagttgttggcagtgctttttgcatactatttgaatttgtggatggttagcagtcagtacactaggatttattactggatctggacagatatgctcgtttcaatcggctcgatttgctatgttgatagctaacatgttgtgcttgtcctactgccctggcgcaaatttgatccatgcattcttttcgttaaatgatttgtcaagtagtaatgcttgtttgaaaatgatattattattattattattattttaaataaatatagcctgaaacattcttagtttcgaggagaagtgatgatgtcttatccataccacatgtctgcatctcatatgatgattaatctacatcatgcactaccatctgatctcctctagctcatacagcttttaacaagttaggcgatagatgtttgctacaaacagtcaactggatttgctttcttgaaagaaaaaaaatgaaccgtgtagtttgcaacctgtgatgtaaattaacccatttactgaactacattgattatttcagtaaaatcaccatctttcggctgaggttgcatgcttagttattatatttttaccaatgatgattttgttatgtagccagatactgtactttcttatgccatttggtatctgactcattgtctacttttgtgactatgtgtctcgaaatatggcatataataagggggaacggatcttgcgacattgatggctcctgtatgagcctccaattactatatagccgtttacatgttcttatcatgttacttttccaaattagtgctgcacatcttgtctgtttgcaagtttgggttcattcatattgctgtgggcattttaatacatcttaactataatgtaaatttcttgtacatcgctaaaattttctgccttgattgagtagactcttagatgacatctatcttaccttatgcacttgattcgtatttctgttacaacattttgattacttgatgtagttcacacaagcatgacacatctctttgaaaaaaagatttcattttttaaagcaagcctaatgctttagggtgggagaagtgatgatacttaatccatacaacatttctgcatctcagatgatgatcaatctacatcatgcactaccatctgatctccccatcatacaattttttattttgttttgttggattatataactttaacatttgataaagtgccaacagatttttatacacaatttcttatagatattaccatgacagcttgcagcctgtgatgcaaaagtacccattaaactgaattacactgattattccaaacaaatcaccatctttcggctgaggttgcttgcttgtttaaaattatgaatgcttcttgcacaaccatttaggactttcggtagtttgtaatgccatttggtatctgacacatggtctgcgcttgtgactatttgtcttgaaatatggcataaaacaagggggaacggatcttgcgacattggtggttcctctatgatcctccaattactatatagctgtttacaatttgttaagttaggagcataccttttgctttgtcatcaaagtcttggttcctttgtatttctagttcagattcatttttcttcagcttttgaaacataaactcagtatatttttactgtaattgctgacattgagtcttctctgcagatgccaagcgtctcattggcaggaggtttagcgatgcttctgttcagagtgacattaagctctggcccttcaaggtgattgctggacctggtgacaagcctatgattgttgtccagtacaagggtgaggagaagcagtttgctgcagaagagatctcctccatggtcctcatcaagatgcgtgagattgctgaggcctaccttggcaccaccatcaagaatgccgttgtcactgttcctgcctacttcaatgactcccagaggcaggccaccaaggatgctggagtgattgctggtctcaatgtcatgcgtatcatcaacgagccgactgctgcagctattgcctatggtcttgataagaaggccaccagcgttggtgagaagaatgtcctcatctttgaccttggaggtggtacctttgatgtctccctccttaccattgaggagggtatctttgaggtcaaggccacagctggtgacacccatcttggtggtgaagattttgacaatcgtatggtcaaccactttgtgcaagaattcaagaggaagaacaagaaggatatcactggcaaccccagggctctcaggaggttgaggacagcttgtgagagggcaaagaggaccctgtcctccactgcccagaccaccattgagatcgattccctgtatgagggcatcgacttctactcaaccatcacccgtgccaggtttgaggagctcaacatggatctcttcaggaagtgtatggagcctgtggagaagtgcctcagggatgctaagatggacaagagctctgttcatgatgttgtccttgttggtggctccactaggatcccgagggtgcagcagctcctgcaggatttcttcaacggcaaggagctttgcaagaacatcaacccagatgaggctgttgcttatggtgctgctgtccaggctgccatcttgagtggtgagggcaacgagaaggtccaggacctcctcctgttggatgttacccctctctctctcggtttggagactgctggtggtgtcatgaccgttttgatcccaaggaacaccaccattcccaccaagaaggagcaggtcttctccacctactccgacaaccagcctggtgtgctcatccaggtttatgagggtgagaggaccaggacacgtgacaacaacctgctggggaagtttgagctctctggaatccctcctgctcccaggggtgttccacagatcactgtttgcttcgacattgatgccaatggtatcctgaacgtgtctgctgaggacaagaccaccgggcagaagaacaagatcaccattaccaacgacaagggcaggcttagcaaggaggagattgagaagatggtccaggaggccgagaagtacaagtcagaggatgaggagcacaagaagaaggtggagtccaagaacgcgctggagaactacgcctacaacatgcgcaacaccatcaaggatgagaagatcgcctcgaagctcccggcagcggacaagaagaagatcgaggatgccatcgaccaggccatccagtggctggacggcaaccagctcgctgaggctgatgagttcgatgacaagatgaaggagctggagggcatctgcaaccccatcatcgccaagatgtaccagggcgctggcgcggacatggccggcggcatggacgaggacgatgctcccccggctggcggcagcggtgctggccccaagatcgaggaggtcgactaagcgcgaaatttgctagtgatccgcgcttcaagttatcttattgcattattagtgtctcttttatgttgtgttaaaaaactttgctgctatgacttatgatgatacccttggtctgtttgggcagtccttttgaattgaagaactgcgttgggagctctatgagaactatgaattgcgtgagaaatttaattattgtggtgcttgcagcgcctattatttggtctctgtcccacgtttattatttgcatgtgcgcattgtttgctagcaattgctgttgtgcttctcagccaacatgtaaactgtttggattgtttctgatttttggatgcattttttaaagcggatagtgaaggacgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001075072.1 RefSeq:Os11g0703900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0703900&diff=182795 2014-06-09T15:14:33Z

<p>Maple: /* Expression */</p> <hr /> <div>Gene Os11g0703900, namely '''Hsp70''',meaning rice heat shock protein gene,is a multigenic family, andregulates the expression of stress response genes, such as the regulation of the heat shock protein transcription factor (heat-shock factor, HSF).<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> *Heat shock proteins (heat shock protein, Hsp) are a class of resistance protein molecules playing an important role in the process of environmental stress&lt;ref name=&quot;ref1&quot; /&gt;&lt;ref name=&quot;ref2&quot; /&gt;&lt;ref name=&quot;ref3&quot; /&gt;.<br /> <br /> *Hsp70 genes encode for a group of highly conserved chaperone proteins across the living systems encompassing bacteria, plants, and animals&lt;ref name=&quot;ref4&quot; /&gt;.<br /> <br /> *Hsp70 can not only prevent proteins aggregation and help inactivated proteins refolding, but also assist the functions of transport and participate in the intracellular signal transduction, such as protein kinase C (protein kinase C, PKC) and protein phosphorylation activity (protein phosphatase)&lt;ref name=&quot;ref5&quot; /&gt;.<br /> <br /> *It is noted that Hsp70 may function as a negative feedback regulator of HSF activity&lt;ref name=&quot;ref6&quot; /&gt;&lt;ref name=&quot;ref7&quot; /&gt;.In this process, Hsp70 binds to denatured proteins and releases HSF leading to its activation during heat stress&lt;ref name=&quot;ref6&quot; /&gt;.<br /> <br /> *Heat stress results in misfolding and aggregation of cellular proteins. Heat shock proteins (Hsp) enable the cells to maintain proper folding of proteins, both in unstressed as well as stressed conditions. Hsp70 genes encode for a group of highly conserved chaperone proteins across the living systems encompassing bacteria, plants, and animals. In the cellular chaperone network, Hsp70 family proteins interconnect other chaperones and play a dominant role in various cell processes.<br /> <br /> ===Expression===<br /> <br /> *Rice Hsp70 superfamily genes are represented by 24 Hsp70 family and 8 Hsp110 family members&lt;ref name=&quot;ref4&quot; /&gt;. <br /> <br /> *In rice, ER Hsp70 (BiP) is found to associate with nascent polypeptides that emerge into the ER lumen during prolamin translocation and facilitate peptide folding and assembly&lt;ref name=&quot;ref9&quot; /&gt;. Over-expression of BiP relieves &quot;ER stress&quot;&lt;ref name=&quot;ref10&quot; /&gt;.<br /> <br /> *Rice mitochondrial Hsp70 has been proposed as suppressor of heat and H2O2-induced programmed cell death&lt;ref name=&quot;ref11&quot; /&gt;. <br /> <br /> [[File:Fig 1.Over-expression of mtHSP70 suppressed heat-induced PCD in rice protoplasts.jpg|right|thumb|360px|Fig 1.Over-expression of mtHSP70 suppressed heat-induced PCD in rice protoplasts..&lt;ref name=&quot;ref11&quot; /&gt;'']]<br /> <br /> [[File:Fig.2. Over-expression of mtHSP70 suppressed H2O2-induced PCD in rice protoplasts.jpg|right|thumb|360px|Fig.2. Over-expression of mtHSP70 suppressed H2O2-induced PCD in rice protoplasts..&lt;ref name=&quot;ref11&quot; /&gt;'']]<br /> <br /> *Hsp70 regulates the expression of stress response genes, such as the regulation of the heat shock protein transcription factor (heat-shock factor, HSF)&lt;ref name=&quot;ref8&quot; /&gt;&lt;ref name=&quot;ref12&quot; /&gt;.<br /> <br /> *Overexpression of Hsp70 will decrease the concentration of Ca2+ intracellular&lt;ref name=&quot;ref13&quot; /&gt;, which usually works as a second messenger to stimulate the activity of protein kinases in downstream when the organism faces an environmental stress&lt;ref name=&quot;ref14&quot; /&gt;.<br /> <br /> *Hsp70 are ATP-dependent chaperones having a conserved ~44-kD N-terminal ATPase domain (also called nucleotide binding domain; NBD), a ~18-kD substrate binding domain (SBD) and a ~10-kD variable C-terminal “lid.”&lt;ref name=&quot;ref4&quot; /&gt;.<br /> <br /> *Hsp70 proteins require two cochaperones in the form of J-domain proteins and nucleotide<br /> exchange factors (NEFs) for their functions. Binding of J-domain proteins stimulates ATP hydrolysis that facilitates trapping of exposed hydrophobic region of substrates and closed conformation of SBD. NEFs participate in ATP–ADP exchange by catalyzing the release of ADP from Hsp70, leading to conversion of open conformation Hsp70 and substrate release.<br /> <br /> *The stress induced expression profile of Hsp70 superfamily genes showed diverse response.<br /> <br /> ===Evolution===<br /> <br /> <br /> *Hsp70 is a multigenic family. Various members constituting this family are present in different cellular compartments. In Saccharomyces cerevisiae, 14 Hsp70 genes representing nine cytosolic, three mitochondrial (Ssc, Ecm, and Ssq), and two ER (Kar2 and Lhs1) isoforms have been identified&lt;ref name=&quot;ref15&quot; /&gt;.In E. coli, there are three Hsp70 genes. Arabidopsis has 18 Hsp70 superfamily members, of which 14 belong to Hsp70 family and 4 belong to Hsp110/SSE family&lt;ref name=&quot;ref16&quot; /&gt;.The C-terminus of organellar Hsp70 proteins is highly conserved and unique for each organelle. The motif for the cytosolic group is EEVD, for the mitochondrion is PEAEYEEAKK and for the plastid is PEGDVIDADFTDSK &lt;ref name=&quot;ref17&quot; /&gt;.The C terminus EEVD motif of cytosolic Hsp70 proteins interacts with co-chaperones that contain several degenerate 34 amino acid repeats, called tetratricopeptide repeats.<br /> <br /> *While Hsp70 genes clustered on clades A, B, and C, clade D contained Hsp110 family genes. Hsp110 family is a subfamily of Hsp70 superfamily, structurally very similar to Hsp70 having N-terminal ATPase domain and C-terminal peptide binding domain and functions as NEFs for Hsp70 family proteins&lt;ref name=&quot;ref5&quot; /&gt;.<br /> <br /> *During heat stress the expression of hsp70 correlates well with increases in internal ion concentrations, and can also be induced by excess salt or ethanol at normal growth temperatures. These results appear to indicate that whereas hsp70 is induced by all stresses that lead to protein denaturation — including heat stress&lt;ref name=&quot;ref18&quot; /&gt;.<br /> <br /> ==Labs working on this gene==<br /> <br /> <br /> *1.Sarkar N K, Kundnani P, Grover A.Department of Plant Molecular Biology, University of Delhi South Campus, N Delhi 110021, India<br /> <br /> *2.Wei ZhangCollege of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China<br /> <br /> *3.Laboratorium voor Genetica, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium<br /> <br /> *4.An Department of Plant Molecular Systems Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 446-701, South Korea<br /> <br /> *5.Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Ahuja I, RCH. De Vos, Bones AM, Hall RD: Plant molecular stress responses face climate change. Trends in Plant Science 2010, Vol. 15, No. 12.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref2&quot;&gt;Wang W, Vinocur B, Shoseyov O, Altman A: Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends in Plant Science 2004, Vol. 9, No. 5. &lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref3&quot;&gt;Timperio AM, Egidi MG, Zolla L: Proteomics applied on plant abiotic stresses: Role of heat shock proteins (HSP). Journal of Proteomics 2008, 71: 391-411.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref4&quot;&gt;Sarkar N K, Kundnani P, Grover A. Functional analysis of Hsp70 superfamily proteins of rice (Oryza sativa)[J]. Cell Stress and Chaperones, 2013, 18(4): 427-437.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref5&quot;&gt; Ding XZ, Tsokos GC, Kiang JG: Overexpression of HSP-70 inhibits the phosphorylation of HSF1 by activating protein phosphatase and inhibiting protein kinase C activity. The FASEB Journal 1998, 12:451–459.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref6&quot;&gt;Shi Y, Mosser D D, Morimoto R I. Molecularchaperones as HSF1-specific transcriptional repressors[J]. Genes &amp; development, 1998, 12(5): 654-666.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref7&quot;&gt;Kim B H, Schöffl F. Interaction between Arabidopsis heat shock transcription factor 1 and 70 kDa heat shock proteins[J]. Journal of experimental botany, 2002, 53(367): 371-375.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref8&quot;&gt;Sarkar N K, Kundnani P, Grover A. Functional analysis of Hsp70 superfamily proteins of rice (Oryza sativa)[J]. Cell Stress and Chaperones, 2013, 18(4): 427-437.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref9&quot;&gt;Muench D G, Wu Y, Zhang Y, et al. Molecular cloning, expression and subcellular localization of a BiP homolog from rice endosperm tissue[J]. Plant and cell physiology, 1997, 38(4): 404-412.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref10&quot;&gt;Snowden C J, Leborgne‐Castel N, Wootton L J, et al. In vivo analysis of the lumenal binding protein (BiP) reveals multiple functions of its ATPase domain[J]. The Plant Journal, 2007, 52(6): 987-1000. &lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref11&quot;&gt;Qi Y, Wang H, Zou Y, et al. Over-expression of mitochondrial heat shock protein 70 suppresses programmed cell death in rice[J]. FEBS letters, 2011, 585(1): 231-239.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref12&quot;&gt; Kim BH, Schöffl F. Interaction between Arabidopsis heat shock transcription factor 1 and 70 kDa heat shock proteins. Journal of Experimental Botany 2002, 53(367):371-375.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref13&quot;&gt; Kiang JG, Ding XZ, McClain DE. Overexpression of HSP-70 attenuates increases in [Ca2+]I and protects human epidermoid A-431 cells after chemical hypoxia. Toxicology and Applied Pharmacology 1998, 149(2):185-194.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref14&quot;&gt;Knight H: Calcium signaling during abiotic stress in plants. International Review of Cytology 2000, 195:269-324.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref15&quot;&gt;Walsh P, Bursać D, Law Y C, et al. The J‐protein family: modulating protein assembly, disassembly and translocation[J]. EMBO reports, 2004, 5(6): 567-571.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref16&quot;&gt; Lin B L, Wang J S, Liu H C, et al. Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana[J]. Cell stress &amp; chaperones, 2001, 6(3): 201.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref17&quot;&gt; Guy C L, Li Q B. The organization and evolution of the spinach stress 70 molecular chaperone gene family[J]. The Plant Cell Online, 1998, 10(4): 539-556.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref18&quot;&gt;Frank Van Breusegem, Rudy Dekeyser, Ana Beatriz Garcia,et al.Heat-inducible rice hsp82 and hsp70 are not always co-regulated[J].Planta, 1994, 193(1): 57-66.&lt;/ref&gt; <br /> &lt;references&gt;<br /> <br /> ==Structured Information==<br /> *Hsp70 are ATP-dependent chaperones having a conserved ~44-kD N-terminal ATPase domain (also called nucleotide binding domain; NBD), a ~18-kD substrate binding<br /> domain (SBD) and a ~10-kD variable C-terminal “lid.” The flexible C-terminal lid assists in holding the substrates at SBD. ATPase domain performs regulatory role and SBD associates to hydrophobic regions exposed in non-native substrates.<br /> {{JaponicaGene|<br /> GeneName = Os11g0703900|<br /> Description = Heat shock protein 70|<br /> Version = NM_001075072.1 GI:115486792 GeneID:4351208|<br /> Length = 4293 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0703900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:30612818..30617110|<br /> CDS = 30612889..30613102,30615017..30616752|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:30612818..30617110<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:30612818..30617110<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcgggcaagggcgagggtccggccatcggcatcgaccttggcacgacctactcgtgcgtgggcgtttggcagcacgaccgcgtggagatcatcgccaacgaccagggcaaccgcaccaccccctcctacgtcggcttcaccgactccgagaggctcatcggagatgctgccaagaaccaggtcgccatgaaccccatcaacaccgtctttgatgccaagcgtctcattggcaggaggtttagcgatgcttctgttcagagtgacattaagctctggcccttcaaggtgattgctggacctggtgacaagcctatgattgttgtccagtacaagggtgaggagaagcagtttgctgcagaagagatctcctccatggtcctcatcaagatgcgtgagattgctgaggcctaccttggcaccaccatcaagaatgccgttgtcactgttcctgcctacttcaatgactcccagaggcaggccaccaaggatgctggagtgattgctggtctcaatgtcatgcgtatcatcaacgagccgactgctgcagctattgcctatggtcttgataagaaggccaccagcgttggtgagaagaatgtcctcatctttgaccttggaggtggtacctttgatgtctccctccttaccattgaggagggtatctttgaggtcaaggccacagctggtgacacccatcttggtggtgaagattttgacaatcgtatggtcaaccactttgtgcaagaattcaagaggaagaacaagaaggatatcactggcaaccccagggctctcaggaggttgaggacagcttgtgagagggcaaagaggaccctgtcctccactgcccagaccaccattgagatcgattccctgtatgagggcatcgacttctactcaaccatcacccgtgccaggtttgaggagctcaacatggatctcttcaggaagtgtatggagcctgtggagaagtgcctcagggatgctaagatggacaagagctctgttcatgatgttgtccttgttggtggctccactaggatcccgagggtgcagcagctcctgcaggatttcttcaacggcaaggagctttgcaagaacatcaacccagatgaggctgttgcttatggtgctgctgtccaggctgccatcttgagtggtgagggcaacgagaaggtccaggacctcctcctgttggatgttacccctctctctctcggtttggagactgctggtggtgtcatgaccgttttgatcccaaggaacaccaccattcccaccaagaaggagcaggtcttctccacctactccgacaaccagcctggtgtgctcatccaggtttatgagggtgagaggaccaggacacgtgacaacaacctgctggggaagtttgagctctctggaatccctcctgctcccaggggtgttccacagatcactgtttgcttcgacattgatgccaatggtatcctgaacgtgtctgctgaggacaagaccaccgggcagaagaacaagatcaccattaccaacgacaagggcaggcttagcaaggaggagattgagaagatggtccaggaggccgagaagtacaagtcagaggatgaggagcacaagaagaaggtggagtccaagaacgcgctggagaactacgcctacaacatgcgcaacaccatcaaggatgagaagatcgcctcgaagctcccggcagcggacaagaagaagatcgaggatgccatcgaccaggccatccagtggctggacggcaaccagctcgctgaggctgatgagttcgatgacaagatgaaggagctggagggcatctgcaaccccatcatcgccaagatgtaccagggcgctggcgcggacatggccggcggcatggacgaggacgatgctcccccggctggcggcagcggtgctggccccaagatcgaggaggtcgactaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSY VGFTDSERLIGDAAKNQVAMNPINTVFDAKRLIGRRFSDASVQSDIKLWPFKVIAGPG DKPMIVVQYKGEEKQFAAEEISSMVLIKMREIAEAYLGTTIKNAVVTVPAYFNDSQRQ ATKDAGVIAGLNVMRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTI EEGIFEVKATAGDTHLGGEDFDNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERA KRTLSSTAQTTIEIDSLYEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDK SSVHDVVLVGGSTRIPRVQQLLQDFFNGKELCKNINPDEAVAYGAAVQAAILSGEGNE KVQDLLLLDVTPLSLGLETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYE GERTRTRDNNLLGKFELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKIT ITNDKGRLSKEEIEKMVQEAEKYKSEDEEHKKKVESKNALENYAYNMRNTIKDEKIAS KLPAADKKKIEDAIDQAIQWLDGNQLAEADEFDDKMKELEGICNPIIAKMYQGAGADM AGGMDEDDAPPAGGSGAGPKIEEVD&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;72..285#2200..3935#ctctcccactcttcctcctccccaaaccctagccgccgccgccggcgtactcgagagaaacatcagcatccatggcgggcaagggcgagggtccggccatcggcatcgaccttggcacgacctactcgtgcgtgggcgtttggcagcacgaccgcgtggagatcatcgccaacgaccagggcaaccgcaccaccccctcctacgtcggcttcaccgactccgagaggctcatcggagatgctgccaagaaccaggtcgccatgaaccccatcaacaccgtctttggtaactacttattccctgctcctcagtcctctctctttctctctctccccccttagatctcatctctaaggtcttcgtcgtcgtcgtatgctagatctactcttgcccagctcgttcagatctgttagatttgtgtgggatgctctaattaattactaccaacttagtagatctggaactcttttgacttgcagatcttgctccactcctgttgcattttgaatctgtgttgatgtgatttaagttgttggcagtgctttttgcatactatttgaatttgtggatggttagcagtcagtacactaggatttattactggatctggacagatatgctcgtttcaatcggctcgatttgctatgttgatagctaacatgttgtgcttgtcctactgccctggcgcaaatttgatccatgcattcttttcgttaaatgatttgtcaagtagtaatgcttgtttgaaaatgatattattattattattattattttaaataaatatagcctgaaacattcttagtttcgaggagaagtgatgatgtcttatccataccacatgtctgcatctcatatgatgattaatctacatcatgcactaccatctgatctcctctagctcatacagcttttaacaagttaggcgatagatgtttgctacaaacagtcaactggatttgctttcttgaaagaaaaaaaatgaaccgtgtagtttgcaacctgtgatgtaaattaacccatttactgaactacattgattatttcagtaaaatcaccatctttcggctgaggttgcatgcttagttattatatttttaccaatgatgattttgttatgtagccagatactgtactttcttatgccatttggtatctgactcattgtctacttttgtgactatgtgtctcgaaatatggcatataataagggggaacggatcttgcgacattgatggctcctgtatgagcctccaattactatatagccgtttacatgttcttatcatgttacttttccaaattagtgctgcacatcttgtctgtttgcaagtttgggttcattcatattgctgtgggcattttaatacatcttaactataatgtaaatttcttgtacatcgctaaaattttctgccttgattgagtagactcttagatgacatctatcttaccttatgcacttgattcgtatttctgttacaacattttgattacttgatgtagttcacacaagcatgacacatctctttgaaaaaaagatttcattttttaaagcaagcctaatgctttagggtgggagaagtgatgatacttaatccatacaacatttctgcatctcagatgatgatcaatctacatcatgcactaccatctgatctccccatcatacaattttttattttgttttgttggattatataactttaacatttgataaagtgccaacagatttttatacacaatttcttatagatattaccatgacagcttgcagcctgtgatgcaaaagtacccattaaactgaattacactgattattccaaacaaatcaccatctttcggctgaggttgcttgcttgtttaaaattatgaatgcttcttgcacaaccatttaggactttcggtagtttgtaatgccatttggtatctgacacatggtctgcgcttgtgactatttgtcttgaaatatggcataaaacaagggggaacggatcttgcgacattggtggttcctctatgatcctccaattactatatagctgtttacaatttgttaagttaggagcataccttttgctttgtcatcaaagtcttggttcctttgtatttctagttcagattcatttttcttcagcttttgaaacataaactcagtatatttttactgtaattgctgacattgagtcttctctgcagatgccaagcgtctcattggcaggaggtttagcgatgcttctgttcagagtgacattaagctctggcccttcaaggtgattgctggacctggtgacaagcctatgattgttgtccagtacaagggtgaggagaagcagtttgctgcagaagagatctcctccatggtcctcatcaagatgcgtgagattgctgaggcctaccttggcaccaccatcaagaatgccgttgtcactgttcctgcctacttcaatgactcccagaggcaggccaccaaggatgctggagtgattgctggtctcaatgtcatgcgtatcatcaacgagccgactgctgcagctattgcctatggtcttgataagaaggccaccagcgttggtgagaagaatgtcctcatctttgaccttggaggtggtacctttgatgtctccctccttaccattgaggagggtatctttgaggtcaaggccacagctggtgacacccatcttggtggtgaagattttgacaatcgtatggtcaaccactttgtgcaagaattcaagaggaagaacaagaaggatatcactggcaaccccagggctctcaggaggttgaggacagcttgtgagagggcaaagaggaccctgtcctccactgcccagaccaccattgagatcgattccctgtatgagggcatcgacttctactcaaccatcacccgtgccaggtttgaggagctcaacatggatctcttcaggaagtgtatggagcctgtggagaagtgcctcagggatgctaagatggacaagagctctgttcatgatgttgtccttgttggtggctccactaggatcccgagggtgcagcagctcctgcaggatttcttcaacggcaaggagctttgcaagaacatcaacccagatgaggctgttgcttatggtgctgctgtccaggctgccatcttgagtggtgagggcaacgagaaggtccaggacctcctcctgttggatgttacccctctctctctcggtttggagactgctggtggtgtcatgaccgttttgatcccaaggaacaccaccattcccaccaagaaggagcaggtcttctccacctactccgacaaccagcctggtgtgctcatccaggtttatgagggtgagaggaccaggacacgtgacaacaacctgctggggaagtttgagctctctggaatccctcctgctcccaggggtgttccacagatcactgtttgcttcgacattgatgccaatggtatcctgaacgtgtctgctgaggacaagaccaccgggcagaagaacaagatcaccattaccaacgacaagggcaggcttagcaaggaggagattgagaagatggtccaggaggccgagaagtacaagtcagaggatgaggagcacaagaagaaggtggagtccaagaacgcgctggagaactacgcctacaacatgcgcaacaccatcaaggatgagaagatcgcctcgaagctcccggcagcggacaagaagaagatcgaggatgccatcgaccaggccatccagtggctggacggcaaccagctcgctgaggctgatgagttcgatgacaagatgaaggagctggagggcatctgcaaccccatcatcgccaagatgtaccagggcgctggcgcggacatggccggcggcatggacgaggacgatgctcccccggctggcggcagcggtgctggccccaagatcgaggaggtcgactaagcgcgaaatttgctagtgatccgcgcttcaagttatcttattgcattattagtgtctcttttatgttgtgttaaaaaactttgctgctatgacttatgatgatacccttggtctgtttgggcagtccttttgaattgaagaactgcgttgggagctctatgagaactatgaattgcgtgagaaatttaattattgtggtgcttgcagcgcctattatttggtctctgtcccacgtttattatttgcatgtgcgcattgtttgctagcaattgctgttgtgcttctcagccaacatgtaaactgtttggattgtttctgatttttggatgcattttttaaagcggatagtgaaggacgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001075072.1 RefSeq:Os11g0703900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0703900&diff=182790 2014-06-09T15:12:59Z

<p>Maple: /* Function */</p> <hr /> <div>Gene Os11g0703900, namely '''Hsp70''',meaning rice heat shock protein gene,is a multigenic family, andregulates the expression of stress response genes, such as the regulation of the heat shock protein transcription factor (heat-shock factor, HSF).<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> *Heat shock proteins (heat shock protein, Hsp) are a class of resistance protein molecules playing an important role in the process of environmental stress&lt;ref name=&quot;ref1&quot; /&gt;&lt;ref name=&quot;ref2&quot; /&gt;&lt;ref name=&quot;ref3&quot; /&gt;.<br /> <br /> *Hsp70 genes encode for a group of highly conserved chaperone proteins across the living systems encompassing bacteria, plants, and animals&lt;ref name=&quot;ref4&quot; /&gt;.<br /> <br /> *Hsp70 can not only prevent proteins aggregation and help inactivated proteins refolding, but also assist the functions of transport and participate in the intracellular signal transduction, such as protein kinase C (protein kinase C, PKC) and protein phosphorylation activity (protein phosphatase)&lt;ref name=&quot;ref5&quot; /&gt;.<br /> <br /> *It is noted that Hsp70 may function as a negative feedback regulator of HSF activity&lt;ref name=&quot;ref6&quot; /&gt;&lt;ref name=&quot;ref7&quot; /&gt;.In this process, Hsp70 binds to denatured proteins and releases HSF leading to its activation during heat stress&lt;ref name=&quot;ref6&quot; /&gt;.<br /> <br /> *Heat stress results in misfolding and aggregation of cellular proteins. Heat shock proteins (Hsp) enable the cells to maintain proper folding of proteins, both in unstressed as well as stressed conditions. Hsp70 genes encode for a group of highly conserved chaperone proteins across the living systems encompassing bacteria, plants, and animals. In the cellular chaperone network, Hsp70 family proteins interconnect other chaperones and play a dominant role in various cell processes.<br /> <br /> ===Expression===<br /> <br /> *Rice Hsp70 superfamily genes are represented by 24 Hsp70 family and 8 Hsp110 family members&lt;ref name=&quot;ref4&quot; /&gt;. <br /> <br /> *In rice, ER Hsp70 (BiP) is found to associate with nascent polypeptides that emerge into the ER lumen during prolamin translocation and facilitate peptide folding and assembly&lt;ref name=&quot;ref9&quot; /&gt;. Over-expression of BiP relieves &quot;ER stress&quot;&lt;ref name=&quot;ref10&quot; /&gt;.<br /> <br /> *Rice mitochondrial Hsp70 has been proposed as suppressor of heat and H2O2-induced programmed cell death&lt;ref name=&quot;ref11&quot; /&gt;. <br /> <br /> [[File:Fig 1.Over-expression of mtHSP70 suppressed heat-induced PCD in rice protoplasts.jpg|right|thumb|360px|Fig 1.Over-expression of mtHSP70 suppressed heat-induced PCD in rice protoplasts..&lt;ref name=&quot;ref11&quot; /&gt;'']]<br /> <br /> [[File:Fig.2. Over-expression of mtHSP70 suppressed H2O2-induced PCD in rice protoplasts.jpg|right|thumb|360px|Fig.2. Over-expression of mtHSP70 suppressed H2O2-induced PCD in rice protoplasts..&lt;ref name=&quot;ref11&quot; /&gt;'']]<br /> <br /> *Hsp70 regulates the expression of stress response genes, such as the regulation of the heat shock protein transcription factor (heat-shock factor, HSF)&lt;ref name=&quot;ref8&quot; /&gt;&lt;ref name=&quot;ref12&quot; /&gt;.<br /> <br /> *Overexpression of Hsp70 will decrease the concentration of Ca2+ intracellular&lt;ref name=&quot;ref13&quot; /&gt;, which usually works as a second messenger to stimulate the activity of protein kinases in downstream when the organism faces an environmental stress&lt;ref name=&quot;ref14&quot; /&gt;.<br /> <br /> ===Evolution===<br /> <br /> <br /> *Hsp70 is a multigenic family. Various members constituting this family are present in different cellular compartments. In Saccharomyces cerevisiae, 14 Hsp70 genes representing nine cytosolic, three mitochondrial (Ssc, Ecm, and Ssq), and two ER (Kar2 and Lhs1) isoforms have been identified&lt;ref name=&quot;ref15&quot; /&gt;.In E. coli, there are three Hsp70 genes. Arabidopsis has 18 Hsp70 superfamily members, of which 14 belong to Hsp70 family and 4 belong to Hsp110/SSE family&lt;ref name=&quot;ref16&quot; /&gt;.The C-terminus of organellar Hsp70 proteins is highly conserved and unique for each organelle. The motif for the cytosolic group is EEVD, for the mitochondrion is PEAEYEEAKK and for the plastid is PEGDVIDADFTDSK &lt;ref name=&quot;ref17&quot; /&gt;.The C terminus EEVD motif of cytosolic Hsp70 proteins interacts with co-chaperones that contain several degenerate 34 amino acid repeats, called tetratricopeptide repeats.<br /> <br /> *While Hsp70 genes clustered on clades A, B, and C, clade D contained Hsp110 family genes. Hsp110 family is a subfamily of Hsp70 superfamily, structurally very similar to Hsp70 having N-terminal ATPase domain and C-terminal peptide binding domain and functions as NEFs for Hsp70 family proteins&lt;ref name=&quot;ref5&quot; /&gt;.<br /> <br /> *During heat stress the expression of hsp70 correlates well with increases in internal ion concentrations, and can also be induced by excess salt or ethanol at normal growth temperatures. These results appear to indicate that whereas hsp70 is induced by all stresses that lead to protein denaturation — including heat stress&lt;ref name=&quot;ref18&quot; /&gt;.<br /> <br /> ==Labs working on this gene==<br /> <br /> <br /> *1.Sarkar N K, Kundnani P, Grover A.Department of Plant Molecular Biology, University of Delhi South Campus, N Delhi 110021, India<br /> <br /> *2.Wei ZhangCollege of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China<br /> <br /> *3.Laboratorium voor Genetica, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium<br /> <br /> *4.An Department of Plant Molecular Systems Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 446-701, South Korea<br /> <br /> *5.Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Ahuja I, RCH. De Vos, Bones AM, Hall RD: Plant molecular stress responses face climate change. Trends in Plant Science 2010, Vol. 15, No. 12.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref2&quot;&gt;Wang W, Vinocur B, Shoseyov O, Altman A: Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends in Plant Science 2004, Vol. 9, No. 5. &lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref3&quot;&gt;Timperio AM, Egidi MG, Zolla L: Proteomics applied on plant abiotic stresses: Role of heat shock proteins (HSP). Journal of Proteomics 2008, 71: 391-411.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref4&quot;&gt;Sarkar N K, Kundnani P, Grover A. Functional analysis of Hsp70 superfamily proteins of rice (Oryza sativa)[J]. Cell Stress and Chaperones, 2013, 18(4): 427-437.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref5&quot;&gt; Ding XZ, Tsokos GC, Kiang JG: Overexpression of HSP-70 inhibits the phosphorylation of HSF1 by activating protein phosphatase and inhibiting protein kinase C activity. The FASEB Journal 1998, 12:451–459.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref6&quot;&gt;Shi Y, Mosser D D, Morimoto R I. Molecularchaperones as HSF1-specific transcriptional repressors[J]. Genes &amp; development, 1998, 12(5): 654-666.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref7&quot;&gt;Kim B H, Schöffl F. Interaction between Arabidopsis heat shock transcription factor 1 and 70 kDa heat shock proteins[J]. Journal of experimental botany, 2002, 53(367): 371-375.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref8&quot;&gt;Sarkar N K, Kundnani P, Grover A. Functional analysis of Hsp70 superfamily proteins of rice (Oryza sativa)[J]. Cell Stress and Chaperones, 2013, 18(4): 427-437.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref9&quot;&gt;Muench D G, Wu Y, Zhang Y, et al. Molecular cloning, expression and subcellular localization of a BiP homolog from rice endosperm tissue[J]. Plant and cell physiology, 1997, 38(4): 404-412.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref10&quot;&gt;Snowden C J, Leborgne‐Castel N, Wootton L J, et al. In vivo analysis of the lumenal binding protein (BiP) reveals multiple functions of its ATPase domain[J]. The Plant Journal, 2007, 52(6): 987-1000. &lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref11&quot;&gt;Qi Y, Wang H, Zou Y, et al. Over-expression of mitochondrial heat shock protein 70 suppresses programmed cell death in rice[J]. FEBS letters, 2011, 585(1): 231-239.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref12&quot;&gt; Kim BH, Schöffl F. Interaction between Arabidopsis heat shock transcription factor 1 and 70 kDa heat shock proteins. Journal of Experimental Botany 2002, 53(367):371-375.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref13&quot;&gt; Kiang JG, Ding XZ, McClain DE. Overexpression of HSP-70 attenuates increases in [Ca2+]I and protects human epidermoid A-431 cells after chemical hypoxia. Toxicology and Applied Pharmacology 1998, 149(2):185-194.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref14&quot;&gt;Knight H: Calcium signaling during abiotic stress in plants. International Review of Cytology 2000, 195:269-324.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref15&quot;&gt;Walsh P, Bursać D, Law Y C, et al. The J‐protein family: modulating protein assembly, disassembly and translocation[J]. EMBO reports, 2004, 5(6): 567-571.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref16&quot;&gt; Lin B L, Wang J S, Liu H C, et al. Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana[J]. Cell stress &amp; chaperones, 2001, 6(3): 201.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref17&quot;&gt; Guy C L, Li Q B. The organization and evolution of the spinach stress 70 molecular chaperone gene family[J]. The Plant Cell Online, 1998, 10(4): 539-556.&lt;/ref&gt; <br /> <br /> &lt;ref name=&quot;ref18&quot;&gt;Frank Van Breusegem, Rudy Dekeyser, Ana Beatriz Garcia,et al.Heat-inducible rice hsp82 and hsp70 are not always co-regulated[J].Planta, 1994, 193(1): 57-66.&lt;/ref&gt; <br /> &lt;references&gt;<br /> <br /> ==Structured Information==<br /> *Hsp70 are ATP-dependent chaperones having a conserved ~44-kD N-terminal ATPase domain (also called nucleotide binding domain; NBD), a ~18-kD substrate binding<br /> domain (SBD) and a ~10-kD variable C-terminal “lid.” The flexible C-terminal lid assists in holding the substrates at SBD. ATPase domain performs regulatory role and SBD associates to hydrophobic regions exposed in non-native substrates.<br /> {{JaponicaGene|<br /> GeneName = Os11g0703900|<br /> Description = Heat shock protein 70|<br /> Version = NM_001075072.1 GI:115486792 GeneID:4351208|<br /> Length = 4293 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0703900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:30612818..30617110|<br /> CDS = 30612889..30613102,30615017..30616752|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:30612818..30617110<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:30612818..30617110<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggcgggcaagggcgagggtccggccatcggcatcgaccttggcacgacctactcgtgcgtgggcgtttggcagcacgaccgcgtggagatcatcgccaacgaccagggcaaccgcaccaccccctcctacgtcggcttcaccgactccgagaggctcatcggagatgctgccaagaaccaggtcgccatgaaccccatcaacaccgtctttgatgccaagcgtctcattggcaggaggtttagcgatgcttctgttcagagtgacattaagctctggcccttcaaggtgattgctggacctggtgacaagcctatgattgttgtccagtacaagggtgaggagaagcagtttgctgcagaagagatctcctccatggtcctcatcaagatgcgtgagattgctgaggcctaccttggcaccaccatcaagaatgccgttgtcactgttcctgcctacttcaatgactcccagaggcaggccaccaaggatgctggagtgattgctggtctcaatgtcatgcgtatcatcaacgagccgactgctgcagctattgcctatggtcttgataagaaggccaccagcgttggtgagaagaatgtcctcatctttgaccttggaggtggtacctttgatgtctccctccttaccattgaggagggtatctttgaggtcaaggccacagctggtgacacccatcttggtggtgaagattttgacaatcgtatggtcaaccactttgtgcaagaattcaagaggaagaacaagaaggatatcactggcaaccccagggctctcaggaggttgaggacagcttgtgagagggcaaagaggaccctgtcctccactgcccagaccaccattgagatcgattccctgtatgagggcatcgacttctactcaaccatcacccgtgccaggtttgaggagctcaacatggatctcttcaggaagtgtatggagcctgtggagaagtgcctcagggatgctaagatggacaagagctctgttcatgatgttgtccttgttggtggctccactaggatcccgagggtgcagcagctcctgcaggatttcttcaacggcaaggagctttgcaagaacatcaacccagatgaggctgttgcttatggtgctgctgtccaggctgccatcttgagtggtgagggcaacgagaaggtccaggacctcctcctgttggatgttacccctctctctctcggtttggagactgctggtggtgtcatgaccgttttgatcccaaggaacaccaccattcccaccaagaaggagcaggtcttctccacctactccgacaaccagcctggtgtgctcatccaggtttatgagggtgagaggaccaggacacgtgacaacaacctgctggggaagtttgagctctctggaatccctcctgctcccaggggtgttccacagatcactgtttgcttcgacattgatgccaatggtatcctgaacgtgtctgctgaggacaagaccaccgggcagaagaacaagatcaccattaccaacgacaagggcaggcttagcaaggaggagattgagaagatggtccaggaggccgagaagtacaagtcagaggatgaggagcacaagaagaaggtggagtccaagaacgcgctggagaactacgcctacaacatgcgcaacaccatcaaggatgagaagatcgcctcgaagctcccggcagcggacaagaagaagatcgaggatgccatcgaccaggccatccagtggctggacggcaaccagctcgctgaggctgatgagttcgatgacaagatgaaggagctggagggcatctgcaaccccatcatcgccaagatgtaccagggcgctggcgcggacatggccggcggcatggacgaggacgatgctcccccggctggcggcagcggtgctggccccaagatcgaggaggtcgactaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSY VGFTDSERLIGDAAKNQVAMNPINTVFDAKRLIGRRFSDASVQSDIKLWPFKVIAGPG DKPMIVVQYKGEEKQFAAEEISSMVLIKMREIAEAYLGTTIKNAVVTVPAYFNDSQRQ ATKDAGVIAGLNVMRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTI EEGIFEVKATAGDTHLGGEDFDNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERA KRTLSSTAQTTIEIDSLYEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDK SSVHDVVLVGGSTRIPRVQQLLQDFFNGKELCKNINPDEAVAYGAAVQAAILSGEGNE KVQDLLLLDVTPLSLGLETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYE GERTRTRDNNLLGKFELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKIT ITNDKGRLSKEEIEKMVQEAEKYKSEDEEHKKKVESKNALENYAYNMRNTIKDEKIAS KLPAADKKKIEDAIDQAIQWLDGNQLAEADEFDDKMKELEGICNPIIAKMYQGAGADM AGGMDEDDAPPAGGSGAGPKIEEVD&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;72..285#2200..3935#ctctcccactcttcctcctccccaaaccctagccgccgccgccggcgtactcgagagaaacatcagcatccatggcgggcaagggcgagggtccggccatcggcatcgaccttggcacgacctactcgtgcgtgggcgtttggcagcacgaccgcgtggagatcatcgccaacgaccagggcaaccgcaccaccccctcctacgtcggcttcaccgactccgagaggctcatcggagatgctgccaagaaccaggtcgccatgaaccccatcaacaccgtctttggtaactacttattccctgctcctcagtcctctctctttctctctctccccccttagatctcatctctaaggtcttcgtcgtcgtcgtatgctagatctactcttgcccagctcgttcagatctgttagatttgtgtgggatgctctaattaattactaccaacttagtagatctggaactcttttgacttgcagatcttgctccactcctgttgcattttgaatctgtgttgatgtgatttaagttgttggcagtgctttttgcatactatttgaatttgtggatggttagcagtcagtacactaggatttattactggatctggacagatatgctcgtttcaatcggctcgatttgctatgttgatagctaacatgttgtgcttgtcctactgccctggcgcaaatttgatccatgcattcttttcgttaaatgatttgtcaagtagtaatgcttgtttgaaaatgatattattattattattattattttaaataaatatagcctgaaacattcttagtttcgaggagaagtgatgatgtcttatccataccacatgtctgcatctcatatgatgattaatctacatcatgcactaccatctgatctcctctagctcatacagcttttaacaagttaggcgatagatgtttgctacaaacagtcaactggatttgctttcttgaaagaaaaaaaatgaaccgtgtagtttgcaacctgtgatgtaaattaacccatttactgaactacattgattatttcagtaaaatcaccatctttcggctgaggttgcatgcttagttattatatttttaccaatgatgattttgttatgtagccagatactgtactttcttatgccatttggtatctgactcattgtctacttttgtgactatgtgtctcgaaatatggcatataataagggggaacggatcttgcgacattgatggctcctgtatgagcctccaattactatatagccgtttacatgttcttatcatgttacttttccaaattagtgctgcacatcttgtctgtttgcaagtttgggttcattcatattgctgtgggcattttaatacatcttaactataatgtaaatttcttgtacatcgctaaaattttctgccttgattgagtagactcttagatgacatctatcttaccttatgcacttgattcgtatttctgttacaacattttgattacttgatgtagttcacacaagcatgacacatctctttgaaaaaaagatttcattttttaaagcaagcctaatgctttagggtgggagaagtgatgatacttaatccatacaacatttctgcatctcagatgatgatcaatctacatcatgcactaccatctgatctccccatcatacaattttttattttgttttgttggattatataactttaacatttgataaagtgccaacagatttttatacacaatttcttatagatattaccatgacagcttgcagcctgtgatgcaaaagtacccattaaactgaattacactgattattccaaacaaatcaccatctttcggctgaggttgcttgcttgtttaaaattatgaatgcttcttgcacaaccatttaggactttcggtagtttgtaatgccatttggtatctgacacatggtctgcgcttgtgactatttgtcttgaaatatggcataaaacaagggggaacggatcttgcgacattggtggttcctctatgatcctccaattactatatagctgtttacaatttgttaagttaggagcataccttttgctttgtcatcaaagtcttggttcctttgtatttctagttcagattcatttttcttcagcttttgaaacataaactcagtatatttttactgtaattgctgacattgagtcttctctgcagatgccaagcgtctcattggcaggaggtttagcgatgcttctgttcagagtgacattaagctctggcccttcaaggtgattgctggacctggtgacaagcctatgattgttgtccagtacaagggtgaggagaagcagtttgctgcagaagagatctcctccatggtcctcatcaagatgcgtgagattgctgaggcctaccttggcaccaccatcaagaatgccgttgtcactgttcctgcctacttcaatgactcccagaggcaggccaccaaggatgctggagtgattgctggtctcaatgtcatgcgtatcatcaacgagccgactgctgcagctattgcctatggtcttgataagaaggccaccagcgttggtgagaagaatgtcctcatctttgaccttggaggtggtacctttgatgtctccctccttaccattgaggagggtatctttgaggtcaaggccacagctggtgacacccatcttggtggtgaagattttgacaatcgtatggtcaaccactttgtgcaagaattcaagaggaagaacaagaaggatatcactggcaaccccagggctctcaggaggttgaggacagcttgtgagagggcaaagaggaccctgtcctccactgcccagaccaccattgagatcgattccctgtatgagggcatcgacttctactcaaccatcacccgtgccaggtttgaggagctcaacatggatctcttcaggaagtgtatggagcctgtggagaagtgcctcagggatgctaagatggacaagagctctgttcatgatgttgtccttgttggtggctccactaggatcccgagggtgcagcagctcctgcaggatttcttcaacggcaaggagctttgcaagaacatcaacccagatgaggctgttgcttatggtgctgctgtccaggctgccatcttgagtggtgagggcaacgagaaggtccaggacctcctcctgttggatgttacccctctctctctcggtttggagactgctggtggtgtcatgaccgttttgatcccaaggaacaccaccattcccaccaagaaggagcaggtcttctccacctactccgacaaccagcctggtgtgctcatccaggtttatgagggtgagaggaccaggacacgtgacaacaacctgctggggaagtttgagctctctggaatccctcctgctcccaggggtgttccacagatcactgtttgcttcgacattgatgccaatggtatcctgaacgtgtctgctgaggacaagaccaccgggcagaagaacaagatcaccattaccaacgacaagggcaggcttagcaaggaggagattgagaagatggtccaggaggccgagaagtacaagtcagaggatgaggagcacaagaagaaggtggagtccaagaacgcgctggagaactacgcctacaacatgcgcaacaccatcaaggatgagaagatcgcctcgaagctcccggcagcggacaagaagaagatcgaggatgccatcgaccaggccatccagtggctggacggcaaccagctcgctgaggctgatgagttcgatgacaagatgaaggagctggagggcatctgcaaccccatcatcgccaagatgtaccagggcgctggcgcggacatggccggcggcatggacgaggacgatgctcccccggctggcggcagcggtgctggccccaagatcgaggaggtcgactaagcgcgaaatttgctagtgatccgcgcttcaagttatcttattgcattattagtgtctcttttatgttgtgttaaaaaactttgctgctatgacttatgatgatacccttggtctgtttgggcagtccttttgaattgaagaactgcgttgggagctctatgagaactatgaattgcgtgagaaatttaattattgtggtgcttgcagcgcctattatttggtctctgtcccacgtttattatttgcatgtgcgcattgtttgctagcaattgctgttgtgcttctcagccaacatgtaaactgtttggattgtttctgatttttggatgcattttttaaagcggatagtgaaggacgg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001075072.1 RefSeq:Os11g0703900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=180383 2014-06-07T16:41:37Z

<p>Maple: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues[1].<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=174114 2014-05-29T15:28:54Z

<p>Maple: /* Structured Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> Detailed analysis of the amino acid sequence has revealed<br /> that Hsp100 members contain several conserved signatures.<br /> Schirmer et al divided Hsp100 into class I (Hsp100<br /> types A–D; containing two nucleotide-binding domains)<br /> and class II (Hsp100 types M, N, X, and Y; containing one<br /> nucleotide-binding domain) proteins. The molecular weights<br /> of the Hsp100 proteins is considered to vary between 75 and<br /> 100 kDa because of the size of the non-conserved spacers<br /> between the 2 domains and additional sequences at the Cand<br /> N-termini. Basically, Hsp100 proteins are composed of<br /> fi ve specifi c domains as follows: (i) amino (N) - terminal<br /> domain, (ii) nucleotide – binding domain 1 (NBD1), (iii)<br /> middle domain, (iv) NBD2 and (v) carboxyl (C) - terminal<br /> domain. The conserved sequences of the fi ve domains<br /> include (i) signature sequence I in the N-terminal domain,<br /> (ii) Walker A, Walker B1, and Walker B2 sequences in the<br /> NBD1, (iii) signature sequence II and signature sequence<br /> III in the middle domain, (iv) Walker A and Walker B<br /> sequences in NBD2 and (v) signature sequence IV and V in<br /> the C-terminal domain. Based on more<br /> extensive analysis with larger spectrum of plant species,<br /> Agarwal et al reported further specifi c alterations in<br /> the amino acid sequence of various motifs of plant Hsp100<br /> members.<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=174113 2014-05-29T15:24:31Z

<p>Maple: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> Hsp100 transcript/protein expression is induced during heat<br /> stress in Arabidopsis, soybean, rice, maize, tobacco and wheat. There are also indications that Hsp100 transcript/<br /> protein is induced by desiccation (Shen et al 2003), low<br /> temperature and ABA.<br /> Apart from stress-regulation, Hsp100 transcript and protein<br /> are developmentally regulated in plants. In non-stressed maize, Hsp100 is expressed to a high<br /> level in the tassel at the pre-meiosis stage, the ear (including<br /> silks) and the developing endosperm and embryo.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=174112 2014-05-29T15:13:11Z

<p>Maple: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> HSP100 proteins belong to ClpB family. Clp<br /> ATPases maintain quality of cellular proteins by performing<br /> the function of molecular chaperones and<br /> energy dependent proteases. Clp (Caseinolytic Protease)<br /> system was first identified as a heat shock inducible,<br /> multicomponent, ATP-dependent protease complex able<br /> to hydrolyze casein. Subsequent studies showed<br /> that the Clp system can hydrolyze numerous other proteins<br /> and peptides in both aggregated and non-aggregated<br /> forms Clp ATPases fall within the AAA+<br /> superfamily of ATPases associated with a substantially<br /> broader range of biological processes. Class I<br /> ATPases (ClpA, ClpB, ClpC, ClpD) have two ATP binding<br /> domains and class II Clp ATPases (ClpM, ClpN,<br /> ClpX, ClpY) have one ATP binding domain. Clp<br /> proteins are localized in various cellular organelles in<br /> plants. Basically, Clp system members include three<br /> non-homologous gene families: ClpABCXY, ClpP and<br /> ClpQ. ClpACX members (but not ClpB) facilitate the<br /> activity of ClpP and some, such as ClpA and ClpX,can function as independent chaperones in roles<br /> analogous to those of DnaK and DnaJ proteins. In contrast,<br /> ClpP proteolytic subunit exhibits low levels of peptidolytic<br /> activity. Further, when ClpP is complexed with<br /> ClpA, ClpC or ClpX, active holoenzymes which are able<br /> to cleave denatured proteins are formed. ClpAP, ClpXP<br /> and HslUV proteases are similar in design to the eukaryotic<br /> 26 S proteasome, with the ATPase subunits guarding<br /> the entrance to the proteolytic chamber. Clp proteases in bacterial and eukaryotic systems have been<br /> implicated in vital cellular processes such as sporulation,<br /> DNA replication, protein turnover, stress tolerance and<br /> acclimation and regulation of gene expression. ClpB<br /> is different from ClpA, ClpX and HslU as it does not<br /> associate with peptidase subunits. The function of ClpB<br /> is also distinct from that of other Clp ATPases: this protein<br /> is not involved in protein degradation, instead<br /> it disaggregates and reactivates strongly aggregated proteins. The aggregation reversing activity of ClpB<br /> requires cooperation with the HSP70/HSP110 chaperone<br /> machinery.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=174111 2014-05-29T15:00:59Z

<p>Maple: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> HSP100 is<br /> a major heat-regulated protein family in diverse organisms.<br /> Across the living systems, common features of<br /> HSP100 chaperone action include transient interactions<br /> with non-native protein species, in the prevention of<br /> aggregation and promotion of correct folding and<br /> assembly, or in unfolding for translocation or targeting<br /> to proteases. Singla and Grover showed that homologues<br /> of yeast HSP104 protein are expressed in heat<br /> shocked rice seedlings. It was subsequently established<br /> that apart from heat, rice HSP100 expression is developmentally-<br /> controlled as seeds and developing embryos of<br /> rice show high constitutive levels of this protein.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=174105 2014-05-29T14:50:40Z

<p>Maple: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> ClpB-cyt/HSP100 protein acts as chaperone, mediating disaggregation of denatured proteins.<br /> Previous studies have shown that ClpB-cyt/HSP100 gene belongs to the group class I Clp ATPase proteins and<br /> ClpB-cyt/HSP100 transcript is regulated by heat stress and developmental cues.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Maple https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0519700&diff=174099 2014-05-29T14:41:31Z

<p>Maple: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an<br /> important chaperone protein in rice. Cellular expression<br /> of OsClpB-cyt transcript is governed by heat stress, metal<br /> stress, and developmental cues.<br /> ===Function===<br /> Please input function information here.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0519700|<br /> Description = Heat shock protein 101|<br /> Version = NM_001062601.1 GI:115464932 GeneID:4339343|<br /> Length = 3530 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0519700, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:25864878..25868407|<br /> CDS = 25865113..25865412,25865510..25865707,25865792..25866071,25866160..25866964,25867063..25868218&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:25864878..25868407<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctggctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGG ILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQSAQ KKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGD TNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAV VEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKV ILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFER RFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDK AIDLVDEACANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKE LDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLARVADLKYGA LQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLAD RLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQL FDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVE KAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVA RDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAV TDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKD ELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2996..3295#2701..2898#2337..2616#1444..2248#190..1345#aatccactccgcccttcatatgcgcgcaccgctcctcccgttgtttccatcgacagaagagagagttcagatcaaacgccgaagcaattcttccgcaagatttcgagtgatcccatctcaatccaagctaaaccgagagattttgatttgttgctgtagattgattgtgtgaggagaggaggagcagccatgaatccggacaacttcacgcacaagaccaacgaggcgctcgtggcggcgcacgagatagcgtcggaggcggggcacgcgcagctgacgccgctgcacctggtggcggcgctggcggcggacaagggcgggatactccggcaggcgatctcgcaggcgtccggcggggacgccggcgcgccggactcgttcgagcgcgtggtgagcggggcgctcaagaagctgccctcgcagtcgccgccgccggacagcgtgccggcgtccacggcgctgatcaaggtcatccgccgcgcgcagtccgcgcagaagaagcgcggggactcccacctcgccgtcgaccagctgctgctgggcctcctggaggactcgctaatctccgactgcctcaaggaggccggcgtgtccgcggcgcgggtgcgcgccgagctcgagaagctccgtggcggggagggccgcaaggtggagtccgcgtccggcgacaccaacttccaggcgctcaagacctacggccgcgacctcgtcgagcaggccggtaagctcgaccccgtcattggccgcgacgaggagatccgccgcgtcgtgcgcatcctgtcgcgccgcacgaagaacaaccccgtactcatcggcgagcccggcgtcggcaagacggccgtcgtggagggcctcgcgcagcgcatcgtgcgcggggacgtgcccagcaacctgctcgacgtccgcctcatcgcgctcgacatgggcgcgctcgtcgccggcgccaagtaccgcggcgagttcgaagaacggctcaaggccgtgctcaaggaggtggaggaggccgagggcaaggtgatcctcttcatcgacgagatacacctcgtcctcggcgccgggaggacggagggctccatggacgcggccaacctgttcaagccaatgctcgcgaggggccagctccggtgcatcggcgccacgacgctggaggagtaccgcaagtacgtcgagaaggacgccgcgttcgagaggcggttccagcaggtgttcgtcgcggagccgagcgtcccggacaccatcagcatcctgcgaggcctcaaggagaagtacgaggggcaccacggtgtgaggatccaggaccgcgcgctcgtcgtcgccgcgcagctctcggcgaggtacatcatgggtaagttgctcctgccgtccttgccattgccattgccaacctacattgtcaagctcgagttcacattcttgtgccatttctgctctgtaattcttcaggacgccacctgcctgacaaagcgatcgacctcgtcgacgaggcctgcgcgaacgtgagggtgcagctggacagccagccagaggagatcgacaacctcgagaggaagaggattcagctggaggtggagcaccacgcgctggagaaggagaaggacaaggcaagcaaggcccggcttgtcgaggtcaagaaagagctggacgatctgagggacaagctgcagccattgacgatgaagtacaggaaggagaaggagaggatcgacgagatcaggaagctgaagcagcgccgcgaggagctccagttcacgctgcaggaggccgagcgccggatggacctcgcccgcgtcgccgacctcaagtacggcgcgctccaggagatcgacgtcgccatcgcgaagctcgagagcgagacgggggagaacctgatgctcaccgagaccgtcggccccgagcagatcgccgaggtggtgagccgctggaccggcatcccggtgacccggctcggccagaacgacaaggagaggctcgtcggcctcgccgacaggctgcaccagagggtggtcggccaggcggaggccgtgagcgccgtcgccgaggcggtgctgaggtcgcgcgccggacttggccggccgcagcagcccaccggctcgttcctcttcctcggtcccaccggcgtcggcaagaccgagctcgccaaggccctcgccgagcagctgttcgacgacgagaacctgctcgtccgcatcgacatgtccgagtacatggagcagcactccgtcgctcgcctcatcggagcaccacctgggtaagtatcaatcccaatgttgttttcacagttcttgcaaccgaatttcgggtgctgacatgggggaaatttctctctctgaatgaagctacgtcggccatgaggaaggtgggcagctgacggagcaagtgcggcggcggccgtacagcgtgatcctgttcgacgaggtcgagaaggcgcacgtcgcggtgttcaacactctgctccaggttctcgacgacgggaggctcaccgacgggcaaggccgcacggtggacttcaggaacaccgtgatcatcatgacctccaacctcggcgccgagcacctcctcgccggcatggtcggcaagaactccatgaaggtcgcccgtgaccttgtcatgcaggaggtatgtatggccttttgctttccacaaccatggattcatttgcaatgcaatgcgatgctgaattgatttcgatattttgtgcaggtgaggaggcacttccgtcctgagctcctgaaccggctcgacgagattgtgatattcgatcccctgtcccacgagcaactcaggaaggtcgcccgtctccagatgaaggacgtcgccgtccggctcgccgagaggggtgtcgccctggccgtcaccgacgccgcgctggacgtcattctgtctctgtcctatgatccggtacgcgacagcatccaactatcaggtcgaagtttttcaaagaaattctcgtcgaatttgattctaactctctctgtgactctggtactacgtacaggtttatggcgcgaggccgatcaggaggtggatcgagaagagggtggtgacgcagctgtccaagatgctgatccaggaggagatcgacgagaactgcacggtgtacatcgacgcggcgccgcacaaggacgagctggcctacagggtggacaaccgcggggggttggtgaacgccgagacggggcagaagtcggacatcctcatccaggtgcccaacggcgccgccaccgggagcgacgccgcgcaggcggtgaagaagatgaggatcatggaggacgaggacggcatggacgaagagtagtaagtagtagagcgcgtatgtgcgtgttgcgcccccgggcagaggcagaggagggcgccgatcgagctgtgtgaatgaatgtggggtgtctccgttgaataagctgtgaaatatggtgtttttccgatgatgcctttttgtgaagttgttgttctgctaagacgactttcttttgtgaaatttctgatgaactgaagaagtgtaaaaacgtttacgttctggttgagttgagttg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062601.1 RefSeq:Os05g0519700]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

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