http://192.168.164.12:81/ricewiki/api.php?action=feedcontributions&feedformat=atom&user=Matao152RiceWiki - User contributions [en]2026-08-28T12:14:24ZUser contributionsMediaWiki 1.30.0https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=182255Os06g07249002014-06-09T10:13:26Z<p>Matao152: /* Expression */</p>
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<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2" />.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR.ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3" />.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4" />.<br />
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ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
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===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5" />. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6" />.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7" />As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
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==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
<br />
==References==<br />
<references><br />
<ref name="ref1">Jing Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong (2011).Increased Leaf Angle1,a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell,23(12): 4334-4347.</ref><br />
<ref name="ref2">Eichberg J.,and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.</ref><br />
<ref name="ref3">Wang,L.,Xie,W.,Chen,Y.,Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.</ref><br />
<ref name="ref4">Sakamoto T.,et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.</ref><br />
<ref name="ref5">Miao,Y., Laun, T.M., Smykowski, A., and Zentgraf, U.(2007).Arabidopsis MEKK1 can take a short cut: It can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol. Biol. 65:63–76.</ref><br />
<ref name="ref6">Lee, S., Choi, S.C., and An, G.(2008). Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J.54:93–105.</ref><br />
<ref name="ref6"><br />
Wang, L., Xu, Y., Zhang, C., Ma, Q., Joo, S.H., Kim, S.K., Xu, Z., and Chong, K.(2008). OsLIC, a novel CCCH-type zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE3:e3521.</ref><br />
<ref name="ref6"><br />
Tanaka, A., et al.(2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice.Plant Physiol.151:669–680.</ref><br />
<ref name="ref6"><br />
Zhang, S.W., Li, C.H., Cao, J., Zhang, Y.C., Zhang, S.Q., Xia, Y.F.,Sun, D.Y., and Sun, Y.(2009). Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation. Plant Physiol.151:1889–1901. </ref><br />
<ref name="ref7"><br />
Zhao, S.Q., Hu, J., Guo, L.B., Qian, Q., and Xue, H.W.(2010). Rice leaf inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res. 20:935–947.</ref><br />
<br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=181801Os06g07249002014-06-09T05:17:51Z<p>Matao152: /* Labs working on this gene */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1" /><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2" />.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR.ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3" />.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4" />.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5" />. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6" />.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7" />As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
<br />
==References==<br />
<references><br />
<ref name="ref1">Jing Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong (2011).Increased Leaf Angle1,a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell,23(12): 4334-4347.</ref><br />
<ref name="ref2">Eichberg J.,and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.</ref><br />
<ref name="ref3">Wang,L.,Xie,W.,Chen,Y.,Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.</ref><br />
<ref name="ref4">Sakamoto T.,et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.</ref><br />
<ref name="ref5">Miao,Y., Laun, T.M., Smykowski, A., and Zentgraf, U.(2007).Arabidopsis MEKK1 can take a short cut: It can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol. Biol. 65:63–76.</ref><br />
<ref name="ref6">Lee, S., Choi, S.C., and An, G.(2008). Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J.54:93–105.</ref><br />
<ref name="ref6"><br />
Wang, L., Xu, Y., Zhang, C., Ma, Q., Joo, S.H., Kim, S.K., Xu, Z., and Chong, K.(2008). OsLIC, a novel CCCH-type zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE3:e3521.</ref><br />
<ref name="ref6"><br />
Tanaka, A., et al.(2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice.Plant Physiol.151:669–680.</ref><br />
<ref name="ref6"><br />
Zhang, S.W., Li, C.H., Cao, J., Zhang, Y.C., Zhang, S.Q., Xia, Y.F.,Sun, D.Y., and Sun, Y.(2009). Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation. Plant Physiol.151:1889–1901. </ref><br />
<ref name="ref7"><br />
Zhao, S.Q., Hu, J., Guo, L.B., Qian, Q., and Xue, H.W.(2010). Rice leaf inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res. 20:935–947.</ref><br />
<br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=181786Os06g07249002014-06-09T05:07:08Z<p>Matao152: /* References */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1" /><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2" />.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR.ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3" />.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4" />.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5" />. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6" />.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7" />As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==References==<br />
<references><br />
<ref name="ref1">Jing Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong (2011).Increased Leaf Angle1,a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell,23(12): 4334-4347.</ref><br />
<ref name="ref2">Eichberg J.,and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.</ref><br />
<ref name="ref3">Wang,L.,Xie,W.,Chen,Y.,Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.</ref><br />
<ref name="ref4">Sakamoto T.,et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.</ref><br />
<ref name="ref5">Miao,Y., Laun, T.M., Smykowski, A., and Zentgraf, U.(2007).Arabidopsis MEKK1 can take a short cut: It can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol. Biol. 65:63–76.</ref><br />
<ref name="ref6">Lee, S., Choi, S.C., and An, G.(2008). Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J.54:93–105.</ref><br />
<ref name="ref6"><br />
Wang, L., Xu, Y., Zhang, C., Ma, Q., Joo, S.H., Kim, S.K., Xu, Z., and Chong, K.(2008). OsLIC, a novel CCCH-type zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE3:e3521.</ref><br />
<ref name="ref6"><br />
Tanaka, A., et al.(2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice.Plant Physiol.151:669–680.</ref><br />
<ref name="ref6"><br />
Zhang, S.W., Li, C.H., Cao, J., Zhang, Y.C., Zhang, S.Q., Xia, Y.F.,Sun, D.Y., and Sun, Y.(2009). Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation. Plant Physiol.151:1889–1901. </ref><br />
<ref name="ref7"><br />
Zhao, S.Q., Hu, J., Guo, L.B., Qian, Q., and Xue, H.W.(2010). Rice leaf inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res. 20:935–947.</ref><br />
<br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=181782Os06g07249002014-06-09T05:03:10Z<p>Matao152: /* References */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1" /><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2" />.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR.ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3" />.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4" />.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5" />. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6" />.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7" />As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==References==<br />
<references><br />
<ref name="ref1">Jing Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong (2011).Increased Leaf Angle1,a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell,23(12): 4334-4347.</ref><br />
<ref name="ref2">Eichberg J.,and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.</ref><br />
<refname="ref3">Wang,L.,Xie,W.,Chen,Y.,Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.</ref><br />
<ref name="ref4">Sakamoto T.,et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.</ref><br />
<ref name="ref5">Miao,Y., Laun, T.M., Smykowski, A., and Zentgraf, U.(2007).Arabidopsis MEKK1 can take a short cut: It can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol. Biol. 65:63–76.</ref><br />
<ref name="ref6">Lee, S., Choi, S.C., and An, G.(2008). Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J.54:93–105.</ref><br />
<ref name="ref6"><br />
Wang, L., Xu, Y., Zhang, C., Ma, Q., Joo, S.H., Kim, S.K., Xu, Z., and Chong, K.(2008). OsLIC, a novel CCCH-type zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE3:e3521.</ref><br />
<ref name="ref6"><br />
Tanaka, A., et al.(2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice.Plant Physiol.151:669–680.</ref><br />
<ref name="ref6"><br />
Zhang, S.W., Li, C.H., Cao, J., Zhang, Y.C., Zhang, S.Q., Xia, Y.F.,Sun, D.Y., and Sun, Y.(2009). Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation. Plant Physiol.151:1889–1901. </ref><br />
<ref name="ref7"><br />
Zhao, S.Q., Hu, J., Guo, L.B., Qian, Q., and Xue, H.W.(2010). Rice leaf inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res. 20:935–947.</ref><br />
<br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=181779Os06g07249002014-06-09T05:01:55Z<p>Matao152: /* References */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1" /><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2" />.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR.ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3" />.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4" />.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5" />. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6" />.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7" />As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==References==<br />
<references><br />
<ref name="ref1">Jing Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong (2011).Increased Leaf Angle1,a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell,23(12): 4334-4347.</ref><br />
<ref name="ref2’”>Eichberg J.,and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.</ref><br />
<refname="ref3">Wang,L.,Xie,W.,Chen,Y.,Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.</ref><br />
<ref name="ref4">Sakamoto T.,et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.</ref><br />
<ref name="ref5">Miao,Y., Laun, T.M., Smykowski, A., and Zentgraf, U.(2007).Arabidopsis MEKK1 can take a short cut: It can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol. Biol. 65:63–76.</ref><br />
<ref name="ref6">Lee, S., Choi, S.C., and An, G.(2008). Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J.54:93–105.</ref><br />
<ref name="ref6"><br />
Wang, L., Xu, Y., Zhang, C., Ma, Q., Joo, S.H., Kim, S.K., Xu, Z., and Chong, K.(2008). OsLIC, a novel CCCH-type zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE3:e3521.</ref><br />
<ref name="ref6"><br />
Tanaka, A., et al.(2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice.Plant Physiol.151:669–680.</ref><br />
<ref name="ref6"><br />
Zhang, S.W., Li, C.H., Cao, J., Zhang, Y.C., Zhang, S.Q., Xia, Y.F.,Sun, D.Y., and Sun, Y.(2009). Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation. Plant Physiol.151:1889–1901. </ref><br />
<ref name="ref7"><br />
Zhao, S.Q., Hu, J., Guo, L.B., Qian, Q., and Xue, H.W.(2010). Rice leaf inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res. 20:935–947.</ref><br />
<br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=181773Os06g07249002014-06-09T04:57:35Z<p>Matao152: /* References */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1" /><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2" />.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR.ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3" />.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4" />.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5" />. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6" />.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7" />As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==References==<br />
<references><br />
<ref name="ref1">Jing Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong (2011).Increased Leaf Angle1,a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell,23(12): 4334-4347.</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = 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tcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=181771Os06g07249002014-06-09T04:56:29Z<p>Matao152: /* References */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1" /><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2" />.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR.ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3" />.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4" />.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5" />. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6" />.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7" />As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==References==<br />
</references><br />
<ref name="ref1">Jing Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong (2011).Increased Leaf Angle1,a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell,23(12): 4334-4347.</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=181770Os06g07249002014-06-09T04:54:15Z<p>Matao152: /* References */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1" /><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2" />.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR.ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3" />.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4" />.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5" />. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6" />.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7" />As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==References==<br />
</references><br />
<ref name="ref1">Jing Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12): 4334-4347.</ref><br />
</references><br />
<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=181747Os06g07249002014-06-09T04:32:01Z<p>Matao152: /* References */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1" /><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2" />.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR.ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3" />.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4" />.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5" />. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6" />.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7" />As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==References==<br />
<references><br />
1. Jing Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong<br />
Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice<br />
The Plant Cell, 2011, 23(12): 4334-4347<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=181743Os06g07249002014-06-09T04:28:15Z<p>Matao152: /* References */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1" /><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2" />.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR.ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3" />.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4" />.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5" />. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6" />.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7" />As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==References==<br />
<references><br />
1. Jing Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong<br />
Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice<br />
The Plant Cell, 2011, 23(12): 4334-4347<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=181737Os06g07249002014-06-09T04:24:16Z<p>Matao152: /* References */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1" /><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2" />.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR.ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3" />.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4" />.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5" />. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6" />.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7" />As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==References==<br />
<references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=181736Os06g07249002014-06-09T04:23:45Z<p>Matao152: /* References */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1" /><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2" />.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR.ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3" />.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4" />.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
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===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5" />. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6" />.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7" />As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==References==<br />
<references><br />
<br />
1 Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong.Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12):<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=181732Os06g07249002014-06-09T04:22:03Z<p>Matao152: /* References */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1" /><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2" />.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR.ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3" />.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4" />.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5" />. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6" />.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7" />As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==References==<br />
<references><br />
<br />
1 Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong.Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12): <br />
<br />
2 Eichberg J.,and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.</ref><br />
<refname="ref3"/><br />
Wang,L.,Xie,W.,Chen,Y.,Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.</ref><br />
<ref name="ref4"/><br />
Sakamoto T.,et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.</ref><br />
<ref name="ref5"/><br />
Miao,Y., Laun, T.M., Smykowski, A., and Zentgraf, U.(2007).Arabidopsis MEKK1 can take a short cut: It can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol. Biol. 65:63–76.</ref><br />
<ref name="ref6"/><br />
Lee, S., Choi, S.C., and An, G.(2008). Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J.54:93–105.</ref><br />
<ref name="ref6"/><br />
Wang, L., Xu, Y., Zhang, C., Ma, Q., Joo, S.H., Kim, S.K., Xu, Z., and Chong, K.(2008). OsLIC, a novel CCCH-type zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE3:e3521.</ref><br />
<ref name="ref6"/><br />
Tanaka, A., et al.(2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice.Plant Physiol.151:669–680.</ref><br />
<ref name="ref6"/><br />
Zhang, S.W., Li, C.H., Cao, J., Zhang, Y.C., Zhang, S.Q., Xia, Y.F.,Sun, D.Y., and Sun, Y.(2009). Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation. Plant Physiol.151:1889–1901. </ref><br />
<ref name="ref7"/><br />
Zhao, S.Q., Hu, J., Guo, L.B., Qian, Q., and Xue, H.W.(2010). Rice leaf inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res. 20:935–947.</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=181410Os06g07249002014-06-08T14:58:16Z<p>Matao152: </p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1" /><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2" />.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR.ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3" />.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4" />.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5" />. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6" />.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7" />As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==References==<br />
<references><br />
<ref name="ref1"/><br />
Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong.Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12): 4334-4347.</ref><br />
<ref name="ref2"/><br />
Eichberg J.,and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.</ref><br />
<refname="ref3"/><br />
Wang,L.,Xie,W.,Chen,Y.,Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.</ref><br />
<ref name="ref4"/><br />
Sakamoto T.,et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.</ref><br />
<ref name="ref5"/><br />
Miao,Y., Laun, T.M., Smykowski, A., and Zentgraf, U.(2007).Arabidopsis MEKK1 can take a short cut: It can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol. Biol. 65:63–76.</ref><br />
<ref name="ref6"/><br />
Lee, S., Choi, S.C., and An, G.(2008). Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J.54:93–105.</ref><br />
<ref name="ref6"/><br />
Wang, L., Xu, Y., Zhang, C., Ma, Q., Joo, S.H., Kim, S.K., Xu, Z., and Chong, K.(2008). OsLIC, a novel CCCH-type zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE3:e3521.</ref><br />
<ref name="ref6"/><br />
Tanaka, A., et al.(2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice.Plant Physiol.151:669–680.</ref><br />
<ref name="ref6"/><br />
Zhang, S.W., Li, C.H., Cao, J., Zhang, Y.C., Zhang, S.Q., Xia, Y.F.,Sun, D.Y., and Sun, Y.(2009). Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation. Plant Physiol.151:1889–1901. </ref><br />
<ref name="ref7"/><br />
Zhao, S.Q., Hu, J., Guo, L.B., Qian, Q., and Xue, H.W.(2010). Rice leaf inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res. 20:935–947.</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=181405Os06g07249002014-06-08T14:55:54Z<p>Matao152: /* Expression */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1" /><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2"/>.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR. ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3"/>.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4"/>.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5"/>. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6"/>.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7"/>As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==References==<br />
<references><br />
<ref name="ref1"/><br />
Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong.Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12): 4334-4347.</ref><br />
<ref name="ref2"/><br />
Eichberg J.,and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.</ref><br />
<refname="ref3"/><br />
Wang,L.,Xie,W.,Chen,Y.,Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.</ref><br />
<ref name="ref4"/><br />
Sakamoto T.,et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.</ref><br />
<ref name="ref5"/><br />
Miao,Y., Laun, T.M., Smykowski, A., and Zentgraf, U.(2007).Arabidopsis MEKK1 can take a short cut: It can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol. Biol. 65:63–76.</ref><br />
<ref name="ref6"/><br />
Lee, S., Choi, S.C., and An, G.(2008). Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J.54:93–105.</ref><br />
<ref name="ref6"/><br />
Wang, L., Xu, Y., Zhang, C., Ma, Q., Joo, S.H., Kim, S.K., Xu, Z., and Chong, K.(2008). OsLIC, a novel CCCH-type zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE3:e3521.</ref><br />
<ref name="ref6"/><br />
Tanaka, A., et al.(2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice.Plant Physiol.151:669–680.</ref><br />
<ref name="ref6"/><br />
Zhang, S.W., Li, C.H., Cao, J., Zhang, Y.C., Zhang, S.Q., Xia, Y.F.,Sun, D.Y., and Sun, Y.(2009). Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation. Plant Physiol.151:1889–1901. </ref><br />
<ref name="ref7"/><br />
Zhao, S.Q., Hu, J., Guo, L.B., Qian, Q., and Xue, H.W.(2010). Rice leaf inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res. 20:935–947.</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=181401Os06g07249002014-06-08T14:52:07Z<p>Matao152: /* references */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1"/><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2"/>.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR. ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3"/>.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4"/>.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5"/>. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6"/>.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7"/>As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==References==<br />
<references><br />
<ref name="ref1"/><br />
Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong.Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12): 4334-4347.</ref><br />
<ref name="ref2"/><br />
Eichberg J.,and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.</ref><br />
<refname="ref3"/><br />
Wang,L.,Xie,W.,Chen,Y.,Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.</ref><br />
<ref name="ref4"/><br />
Sakamoto T.,et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.</ref><br />
<ref name="ref5"/><br />
Miao,Y., Laun, T.M., Smykowski, A., and Zentgraf, U.(2007).Arabidopsis MEKK1 can take a short cut: It can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol. Biol. 65:63–76.</ref><br />
<ref name="ref6"/><br />
Lee, S., Choi, S.C., and An, G.(2008). Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J.54:93–105.</ref><br />
<ref name="ref6"/><br />
Wang, L., Xu, Y., Zhang, C., Ma, Q., Joo, S.H., Kim, S.K., Xu, Z., and Chong, K.(2008). OsLIC, a novel CCCH-type zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE3:e3521.</ref><br />
<ref name="ref6"/><br />
Tanaka, A., et al.(2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice.Plant Physiol.151:669–680.</ref><br />
<ref name="ref6"/><br />
Zhang, S.W., Li, C.H., Cao, J., Zhang, Y.C., Zhang, S.Q., Xia, Y.F.,Sun, D.Y., and Sun, Y.(2009). Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation. Plant Physiol.151:1889–1901. </ref><br />
<ref name="ref7"/><br />
Zhao, S.Q., Hu, J., Guo, L.B., Qian, Q., and Xue, H.W.(2010). Rice leaf inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res. 20:935–947.</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=181396Os06g07249002014-06-08T14:46:38Z<p>Matao152: /* references */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1"/><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2"/>.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR. ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3"/>.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4"/>.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5"/>. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6"/>.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7"/>As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==references==<br />
<ref name="ref1"/> Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong.Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12): 4334-4347.</ref><br />
<ref name="ref2"/>Eichberg J.,and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.</ref><br />
<refname="ref3"/>Wang,L.,Xie,W.,Chen,Y.,Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.</ref><br />
<ref name="ref4"/>Sakamoto T.,et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.</ref><br />
<ref name="ref5"/>Miao,Y., Laun, T.M., Smykowski, A., and Zentgraf, U.(2007).Arabidopsis MEKK1 can take a short cut: It can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol. Biol. 65:63–76.</ref><br />
<ref name="ref6"/>Lee, S., Choi, S.C., and An, G.(2008). Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J.54:93–105.</ref><br />
<ref name="ref6"/>Wang, L., Xu, Y., Zhang, C., Ma, Q., Joo, S.H., Kim, S.K., Xu, Z., and Chong, K.(2008). OsLIC, a novel CCCH-type zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE3:e3521.</ref><br />
<ref name="ref6"/>Tanaka, A., et al.(2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice.Plant Physiol.151:669–680.</ref><br />
<ref name="ref6"/>Zhang, S.W., Li, C.H., Cao, J., Zhang, Y.C., Zhang, S.Q., Xia, Y.F.,Sun, D.Y., and Sun, Y.(2009). Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation. Plant Physiol.151:1889–1901. </ref><br />
<ref name="ref7"/>Zhao, S.Q., Hu, J., Guo, L.B., Qian, Q., and Xue, H.W.(2010). Rice leaf inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res. 20:935–947.</ref><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=181387Os06g07249002014-06-08T14:40:04Z<p>Matao152: /* references */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1"/><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2"/>.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR. ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3"/>.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4"/>.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5"/>. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6"/>.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7"/>As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==references==<br />
<ref name="ref1"/> Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong.Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12): 4334-4347.<ref1><br />
<ref name="ref2"/>Eichberg J.,and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.<ref2><br />
<refname="ref3"/>Wang,L.,Xie,W.,Chen,Y.,Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.<ref3><br />
<ref name="ref4"/>Sakamoto T.,et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.<ref4><br />
<ref name="ref5"/>Miao,Y., Laun, T.M., Smykowski, A., and Zentgraf, U.(2007).Arabidopsis MEKK1 can take a short cut: It can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol. Biol. 65:63–76.<ref5><br />
<ref name="ref6"/>Lee, S., Choi, S.C., and An, G.(2008). Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J.54:93–105.<ref6><br />
<ref name="ref6"/>Wang, L., Xu, Y., Zhang, C., Ma, Q., Joo, S.H., Kim, S.K., Xu, Z., and Chong, K.(2008). OsLIC, a novel CCCH-type zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE3:e3521.<ref6><br />
<ref name="ref6"/>Tanaka, A., et al.(2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice.Plant Physiol.151:669–680.<ref6><br />
<ref name="ref6"/>Zhang, S.W., Li, C.H., Cao, J., Zhang, Y.C., Zhang, S.Q., Xia, Y.F.,Sun, D.Y., and Sun, Y.(2009). Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation. Plant Physiol.151:1889–1901. <ref6><br />
<ref name="ref7"/>Zhao, S.Q., Hu, J., Guo, L.B., Qian, Q., and Xue, H.W.(2010). Rice leaf inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res. 20:935–947.<ref7><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = 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tcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=181383Os06g07249002014-06-08T14:37:30Z<p>Matao152: </p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1"/><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2"/>.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR. ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3"/>.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4"/>.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
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===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5"/>. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6"/>.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7"/>As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==references==<br />
<ref name="ref1"/> Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong.Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12): 4334-4347.<ref/><br />
<ref name="ref2"/>Eichberg J.,and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.<ref/><br />
<refname="ref3"/>Wang,L.,Xie,W.,Chen,Y.,Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.<ref/><br />
<ref name="ref4"/>Sakamoto T.,et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.<ref/><br />
<ref name="ref5"/>Miao,Y., Laun, T.M., Smykowski, A., and Zentgraf, U.(2007).Arabidopsis MEKK1 can take a short cut: It can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol. Biol. 65:63–76.<ref/><br />
<ref name="ref6"/>Lee, S., Choi, S.C., and An, G.(2008). Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J.54:93–105.<ref/><br />
<ref name="ref6"/>Wang, L., Xu, Y., Zhang, C., Ma, Q., Joo, S.H., Kim, S.K., Xu, Z., and Chong, K.(2008). OsLIC, a novel CCCH-type zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE3:e3521.<ref/><br />
<ref name="ref6"/>Tanaka, A., et al.(2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice.Plant Physiol.151:669–680.<ref/><br />
<ref name="ref6"/>Zhang, S.W., Li, C.H., Cao, J., Zhang, Y.C., Zhang, S.Q., Xia, Y.F.,Sun, D.Y., and Sun, Y.(2009). Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation. Plant Physiol.151:1889–1901. <ref/><br />
<ref name="ref7"/>Zhao, S.Q., Hu, J., Guo, L.B., Qian, Q., and Xue, H.W.(2010). Rice leaf inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res. 20:935–947.<ref/><br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=181373Os06g07249002014-06-08T14:33:34Z<p>Matao152: </p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1"/><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2"/>.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR. ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3"/>.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4"/>.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5"/>. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6"/>.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7"/>As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==references==<br />
<ref name="ref1"/> Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong.Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12): 4334-4347.<br />
<ref name="ref2"/>Eichberg J.,and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.<br />
<refname="ref3"/>Wang,L.,Xie,W.,Chen,Y.,Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.<br />
<ref name="ref4"/>Sakamoto T.,et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.<br />
<ref name="ref5"/>Miao,Y., Laun, T.M., Smykowski, A., and Zentgraf, U.(2007).Arabidopsis MEKK1 can take a short cut: It can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol. Biol. 65:63–76.<br />
<ref name="ref6"/>Lee, S., Choi, S.C., and An, G.(2008). Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J.54:93–105.<br />
<ref name="ref6"/>Wang, L., Xu, Y., Zhang, C., Ma, Q., Joo, S.H., Kim, S.K., Xu, Z., and Chong, K.(2008). OsLIC, a novel CCCH-type zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE3:e3521.<br />
<ref name="ref6"/>Tanaka, A., et al.(2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice.Plant Physiol.151:669–680.<br />
<ref name="ref6"/>Zhang, S.W., Li, C.H., Cao, J., Zhang, Y.C., Zhang, S.Q., Xia, Y.F.,Sun, D.Y., and Sun, Y.(2009). Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation. Plant Physiol.151:1889–1901. <br />
<ref name="ref7"/>Zhao, S.Q., Hu, J., Guo, L.B., Qian, Q., and Xue, H.W.(2010). Rice leaf inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res. 20:935–947.<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=180707Os06g07249002014-06-08T05:45:35Z<p>Matao152: </p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
[[File:relative exp.level to UBQ5.jpg]]<ref name="ref1"/><br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2"/>.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR. ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3"/>.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4"/>.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length<br />
of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5"/>. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6"/>.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7"/>As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==references==<br />
<ref name="ref1"/> Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong.Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12): 4334-4347.<br />
<ref name="ref2"/>, J., and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.<br />
<ref name="ref3"/>Wang,L.,Xie,W.,Chen,Y., Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.<br />
<ref name="ref4"/>, T., et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.<br />
<ref name="ref5"/>, Y., Laun, T.M., Smykowski, A., and Zentgraf, U.(2007).Arabidopsis MEKK1 can take a short cut: It can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol. Biol. 65:63–76.<br />
<ref name="ref6"/>Lee, S., Choi, S.C., and An, G.(2008). Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J.54:93–105.<br />
<ref name="ref6"/>Wang, L., Xu, Y., Zhang, C., Ma, Q., Joo, S.H., Kim, S.K., Xu, Z., and Chong, K.(2008). OsLIC, a novel CCCH-type zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE3:e3521.<br />
<ref name="ref6"/>Tanaka, A., et al.(2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice.Plant Physiol.151:669–680.<br />
<ref name="ref6"/>Zhang, S.W., Li, C.H., Cao, J., Zhang, Y.C., Zhang, S.Q., Xia, Y.F.,Sun, D.Y., and Sun, Y.(2009). Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation. Plant Physiol.151:1889–1901. <br />
<ref name="ref7"/>Zhao, S.Q., Hu, J., Guo, L.B., Qian, Q., and Xue, H.W.(2010). Rice leaf<br />
inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res. 20:935–947.<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=180701Os06g07249002014-06-08T05:41:23Z<p>Matao152: /* Expression */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===[[File:relative exp.level to UBQ5.jpg]]<br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2"/>.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR. ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3"/>.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4"/>.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length<br />
of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5"/>. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6"/>.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7"/>As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==references==<br />
<ref name="ref1"/> Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong.Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12): 4334-4347.<br />
<ref name="ref2"/>, J., and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.<br />
<ref name="ref3"/>Wang,L.,Xie,W.,Chen,Y., Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.<br />
<ref name="ref4"/>, T., et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.<br />
<ref name="ref5"/>, Y., Laun, T.M., Smykowski, A., and Zentgraf, U.(2007).Arabidopsis MEKK1 can take a short cut: It can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol. Biol. 65:63–76.<br />
<ref name="ref6"/>Lee, S., Choi, S.C., and An, G.(2008). Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J.54:93–105.<br />
<ref name="ref6"/>Wang, L., Xu, Y., Zhang, C., Ma, Q., Joo, S.H., Kim, S.K., Xu, Z., and Chong, K.(2008). OsLIC, a novel CCCH-type zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE3:e3521.<br />
<ref name="ref6"/>Tanaka, A., et al.(2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice.Plant Physiol.151:669–680.<br />
<ref name="ref6"/>Zhang, S.W., Li, C.H., Cao, J., Zhang, Y.C., Zhang, S.Q., Xia, Y.F.,Sun, D.Y., and Sun, Y.(2009). Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation. Plant Physiol.151:1889–1901. <br />
<ref name="ref7"/>Zhao, S.Q., Hu, J., Guo, L.B., Qian, Q., and Xue, H.W.(2010). Rice leaf<br />
inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res. 20:935–947.<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=180698Os06g07249002014-06-08T05:37:22Z<p>Matao152: </p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2"/>.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR. ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3"/>.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4"/>.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length<br />
of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5"/>. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6"/>.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7"/>As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==references==<br />
<ref name="ref1"/> Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong.Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12): 4334-4347.<br />
<ref name="ref2"/>, J., and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.<br />
<ref name="ref3"/>Wang,L.,Xie,W.,Chen,Y., Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.<br />
<ref name="ref4"/>, T., et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.<br />
<ref name="ref5"/>, Y., Laun, T.M., Smykowski, A., and Zentgraf, U.(2007).Arabidopsis MEKK1 can take a short cut: It can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol. Biol. 65:63–76.<br />
<ref name="ref6"/>Lee, S., Choi, S.C., and An, G.(2008). Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J.54:93–105.<br />
<ref name="ref6"/>Wang, L., Xu, Y., Zhang, C., Ma, Q., Joo, S.H., Kim, S.K., Xu, Z., and Chong, K.(2008). OsLIC, a novel CCCH-type zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE3:e3521.<br />
<ref name="ref6"/>Tanaka, A., et al.(2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice.Plant Physiol.151:669–680.<br />
<ref name="ref6"/>Zhang, S.W., Li, C.H., Cao, J., Zhang, Y.C., Zhang, S.Q., Xia, Y.F.,Sun, D.Y., and Sun, Y.(2009). Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation. Plant Physiol.151:1889–1901. <br />
<ref name="ref7"/>Zhao, S.Q., Hu, J., Guo, L.B., Qian, Q., and Xue, H.W.(2010). Rice leaf<br />
inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res. 20:935–947.<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=180695Os06g07249002014-06-08T05:31:02Z<p>Matao152: /* Annotated Information */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2"/>.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR. ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3"/>.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4"/>.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length<br />
of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5"/>. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6"/>.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7"/>As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==references==<br />
1.Jing Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong.Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12): 4334-4347.<br />
2.Eichberg, J., and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.<br />
3.Wang,L.,Xie,W.,Chen,Y., Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.<br />
4.Sakamoto, T., et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.<br />
5.Miao, Y., Laun, T.M., Smykowski, A., and Zentgraf, U.(2007).Arabidopsis MEKK1 can take a short cut: It can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol. Biol. 65:63–76.<br />
6.Lee, S., Choi, S.C., and An, G.(2008). Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J.54:93–105.<br />
Wang, L., Xu, Y., Zhang, C., Ma, Q., Joo, S.H., Kim, S.K., Xu, Z., and Chong, K.(2008). OsLIC, a novel CCCH-type zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE3:e3521.<br />
Tanaka, A., et al.(2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice.Plant Physiol.151:669–680.<br />
Zhang, S.W., Li, C.H., Cao, J., Zhang, Y.C., Zhang, S.Q., Xia, Y.F.,Sun, D.Y., and Sun, Y.(2009). Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation. Plant Physiol.151:1889–1901. <br />
7.Zhao, S.Q., Hu, J., Guo, L.B., Qian, Q., and Xue, H.W.(2010). Rice leaf<br />
inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res. 20:935–947.<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=180693Os06g07249002014-06-08T05:27:28Z<p>Matao152: </p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2"/>.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR. ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3"/>.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4"/>.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 is a raf-like MAPKKK of Group C.ILA1 encodes a putative MAPKKK protein with a length<br />
of 564 amino acids and a molecular mass of 63 kD.The deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1.<br />
You can also add sub-section(s) at will.<br />
The increased leaf angle of ila1 is not due to altered BR.<br />
The increased leaf inclination cosegregated with the homozygous T-DNA insertion based on PCR analysis.RT-PCR assays show that the intact transcript ofOs06g50920 is undetectable in the ila1 mutant,indicating that the T-DNA insertion nearly represses expression of the targeted gene.BR-induced rice leaf inclination often results from rapid expansion and propagation of collar adaxial cells.Scanning electron microscopy is used to observe the adaxial surface and longitudinal sections ofial1 and wild-type leaf lamina joints. No significant alterations in cell expansion and propagation are found in the adaxial region.So the increased leaf angle of ila1 is not due to altered BR.<br />
Possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5"/>. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6"/>.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7"/>As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==references==<br />
1.Jing Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong.Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12): 4334-4347.<br />
2.Eichberg, J., and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.<br />
3.Wang,L.,Xie,W.,Chen,Y., Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.<br />
4.Sakamoto, T., et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.<br />
5.Miao, Y., Laun, T.M., Smykowski, A., and Zentgraf, U.(2007).Arabidopsis MEKK1 can take a short cut: It can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol. Biol. 65:63–76.<br />
6.Lee, S., Choi, S.C., and An, G.(2008). Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J.54:93–105.<br />
Wang, L., Xu, Y., Zhang, C., Ma, Q., Joo, S.H., Kim, S.K., Xu, Z., and Chong, K.(2008). OsLIC, a novel CCCH-type zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE3:e3521.<br />
Tanaka, A., et al.(2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice.Plant Physiol.151:669–680.<br />
Zhang, S.W., Li, C.H., Cao, J., Zhang, Y.C., Zhang, S.Q., Xia, Y.F.,Sun, D.Y., and Sun, Y.(2009). Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation. Plant Physiol.151:1889–1901. <br />
7.Zhao, S.Q., Hu, J., Guo, L.B., Qian, Q., and Xue, H.W.(2010). Rice leaf<br />
inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res. 20:935–947.<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=180637Os06g07249002014-06-08T03:50:21Z<p>Matao152: </p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint<ref name="ref1"/>.<br />
===Expression===<br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity<ref name="ref2"/>.Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR. ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses <ref name="ref3"/>.ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle<ref name="ref4"/>.<br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely Targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
ILA1 Is a RAF-Like MAPKKK of Group C.<br />
<br />
You can also add sub-section(s) at will.<br />
possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref5"/>. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref6"/>.Some, not related to BR responses, also function in cell division or expansion.<ref name="ref7"/>As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.)<br />
# Institute of Society for Techno-Innovation of Agriculture,Forestry and Fisheries,Tsukuba, Japan (Z.S.)<br />
# Faculty of Agriculture, Utsunomiya University, Utsunomiya,Japan (C.T., H.S.)<br />
# Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); <br />
# Department of Applied Biological Chemistry, University of Tokyo,Tokyo, Japan (T.A.)<br />
==references==<br />
1.Jing Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong.Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12): 4334-4347.<br />
2.Eichberg, J., and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.<br />
3.Wang,L.,Xie,W.,Chen,Y., Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.<br />
4.Sakamoto, T., et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.<br />
5.Miao, Y., Laun, T.M., Smykowski, A., and Zentgraf, U.(2007).Arabidopsis MEKK1 can take a short cut: It can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol. Biol. 65:63–76.<br />
6.Lee, S., Choi, S.C., and An, G.(2008). Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J.54:93–105.<br />
Wang, L., Xu, Y., Zhang, C., Ma, Q., Joo, S.H., Kim, S.K., Xu, Z., and Chong, K.(2008). OsLIC, a novel CCCH-type zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE3:e3521.<br />
Tanaka, A., et al.(2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice.Plant Physiol.151:669–680.<br />
Zhang, S.W., Li, C.H., Cao, J., Zhang, Y.C., Zhang, S.Q., Xia, Y.F.,Sun, D.Y., and Sun, Y.(2009). Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation. Plant Physiol.151:1889–1901. <br />
7.Zhao, S.Q., Hu, J., Guo, L.B., Qian, Q., and Xue, H.W.(2010). Rice leaf<br />
inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res. 20:935–947.<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=180598Os06g07249002014-06-08T03:12:41Z<p>Matao152: </p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint.<ref name="ref1"/><br />
===Expression===<br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity.<ref name="ref2"/>Thermal asymmetric interlaced PCR show that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR. ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses. <ref name="ref3"/ILA1 regulates mechanical strength in the leaf lamina joint.Genome-wide exploration of the expression profiles of ila1 and wild-type leaf lamina joints showed that the expressionof many genes involved in cell wall formation is downregulated in the mutants.Rice leaf angle is one of the important agronomic traits<br />
affecting plant architecture and yield. Most of the previously documented rice leaf inclination mutants arise from BR-induced cell division and/or elongation at the adaxial surface of the leaf lamina joint. Suppression of BR biosynthesis or signaling generally blocks cell propagation/expansion and results in an erect leaf; in the presence of BR, cell division/expansion occurs and induces an increased leaf angle.<ref name="ref4"/><br />
<br />
ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the likely Targets of ILA1, are nuclear proteins with ransactivation activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
<br />
<br />
You can also add sub-section(s) at will.<br />
possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. In fact, direct phosphorylation of a transcription factor by a MAPKKK has been previously reported<ref name="ref4"/>. Many transcription factors have been found that are involved in modulating rice leaf inclination. Most of them act through BR signaling pathways <ref name="ref5"/> Some, not related to BR responses, also function in cell division or expansion.<ref name="ref6"/>As the interacting proteins of ILA1, IIPs appear to regulate directly or indirectly multiple aspects of cell wall–related gene expression in the leaf lamina joint.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
# Disease Resistance Research Unit,Plant Genome Research Unit (S.K.),National Institute of Agrobiological Sciences, Tsukuba,Japan<br />
# Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan (A.T., T.K.); Institute of Society for Techno-Innovation of Agriculture, Forestry and Fisheries, Tsukuba 305–0854, Japan (Z.S.); Faculty of Agriculture, Utsunomiya University, Utsunomiya 321–8505, Japan (C.T., H.S.); Department of Bioscience, Teikyo University, Utsunomiya 320–8551, Japan (T.N., T.Y.); and Department of Applied Biological Chemistry, University of Tokyo, Tokyo 113–8657, Japan (T.A.)<br />
==references==<br />
1.Jing Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong.Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12): 4334-4347.<br />
2.Eichberg, J., and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.<br />
3.Wang,L.,Xie,W.,Chen,Y., Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.<br />
4.Sakamoto, T., et al.(2006). Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat.Biotechnol.24:105–109.<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=180547Os06g07249002014-06-08T02:48:48Z<p>Matao152: </p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint.<ref name="ref1"/><br />
===Expression===<br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity.<ref name="ref2"/>Thermal asymmetric interlaced PCR showed that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR. ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses. <ref name="ref3"/>ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the Likely Targets of ILA1, are Nuclear Proteins with Transactivation Activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
<br />
===Evolution===<br />
<br />
<br />
You can also add sub-section(s) at will.<br />
possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. <br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
<br />
==references==<br />
1.Jing Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong.Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12): 4334-4347<br />
2.Eichberg, J., and Iyer, S.(1996). Phosphorylation of myelin protein:Recent advances. Neurochem. Res.21:527–535.3.<br />
3.Wang,L.,Xie,W.,Chen,Y., Tang,W.,Yang,J.,Ye,R.,Liu,L.,Lin,Y.,Xu, C., Xiao, J.,and Zhang,Q.(2010).A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=180515Os06g07249002014-06-08T02:38:43Z<p>Matao152: </p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint.<ref name="ref1" / ><br />
===Expression===<br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.ILA1Encodes a Group C Raf-Like MAPKKK with Ser/Thr Kinase Activity.Thermal asymmetric interlaced PCR showed that the increased leaf angle ofila1arises from a T-DNA insertion in Os06g50920.Because nuclear localization is a significant feature of transcription factors, GFP is fused to the N terminus of IIP2 and IIP4 and transformed the rice protoplasts. Detection of thefusion proteins in the nuclei of the transformed cells suggest that IIP2 and IIP4 are nuclear-localized proteins. To confirm the sequence identity ofILA1, a complementation test is performed by transforming theila1 mutant with an 8.8-kbgenomic region that included the complete open reading frame(ORF) and putative promoter region for ILA1 (pILA1). All of the complementation lines show the wild-type leaf inclination,suggesting that Os06g50920 is ILA1.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR. ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses. <ref name="ref2"/>ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species reveal that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis reveal high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. To confirm the interaction of ILA1 with IIPs, the ILA1 protein is split into kinase (ILA1K) and regulatory (ILA1R) domains andsubjected to interaction analysis.The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A.And the N-terminal regulatory domain interacts with IIPs.<br />
IIPs,the Likely Targets of ILA1, are Nuclear Proteins with Transactivation Activity.In light of the evidence for interaction between ILA1 and IIPs, it seemed that IIPs may be the phosphorylated substrates of ILA1.IIP4 is selected as a representative to test this hypothesis.IIP4 fused to a polyhistidine tag (His-IIP4)is purified and incubated<br />
with GST-ILA1 for an in vitro kinase activity assay. Radioactively labeled IIP4 is detected. These results reveal that IIP4 is a phosphorylated substrate of ILA1, as is likely the case for the other IIPs.The Gene Ontology database annotate IIPs as putative transcription factors.<br />
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===Evolution===<br />
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You can also add sub-section(s) at will.<br />
possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. <br />
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==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
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==references==<br />
1.Jing Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong.Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12): 4334-4347<br />
2.Wang, L., Xie, W., Chen, Y., Tang, W., Yang, J., Ye, R., Liu, L., Lin, Y.,Xu, C., Xiao, J., and Zhang, Q.(2010). A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.<br />
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==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=173858Os06g07249002014-05-29T04:47:57Z<p>Matao152: </p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint.<ref name="ref1" / ><br />
===Expression===<br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR. ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses. <ref name="ref2"/>ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species revealed that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis revealed high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. <br />
<br />
===Evolution===<br />
MAPKKKs generally phosphorylate target proteins to mediate signal transduction.ILA1 Interacts with a Functionally Unidentified Protein Family. The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A. <br />
<br />
You can also add sub-section(s) at will.<br />
possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. <br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
<br />
==References==<br />
1.Jing Ning;Baocai Zhang;Nili Wang;Yihua Zhou;Lizhong Xiong.Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.The Plant Cell, 2011, 23(12): 4334-4347<br />
2.Wang, L., Xie, W., Chen, Y., Tang, W., Yang, J., Ye, R., Liu, L., Lin, Y.,Xu, C., Xiao, J., and Zhang, Q.(2010). A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Relative_exp.level_to_UBQ5.pdf..jpg&diff=173678File:Relative exp.level to UBQ5.pdf..jpg2014-05-28T13:49:26Z<p>Matao152: </p>
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<div></div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=173671Os06g07249002014-05-28T13:28:50Z<p>Matao152: </p>
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<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
ILA1 is a functional kinase with Ser/Thr kinase activity.ILA1 is a key factor regulating mechanical tissue formation at the leaf lamina joint and is involved in mechanical tissue formation in the leaf lamina joint.<ref name="ref1" / ><br />
===Expression===<br />
ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints.The leaf angle of WT is increased compared that of ila1. But the increased leaf angle of ila1 is not due to altered BR. ILA1 mutation significantly represses the expression of genes involved in cell wall synthesis and consequently causes the increased leaf responses. <ref name="ref2"/>ILA1 encodes a putative MAPKKK protein with a length of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of ILA1 with several identified MAPKKKs from three representative plant species revealed that the characteristic features for MAPKKK proteins, including all of the 11 subdomains and a Lys in the ATP binding site, are highly conserved in ILA1. ILA1 represents a potentially unique paradigm in the regulation of leaf angle in rice. QRT-PCR analysis revealed high expression levels of ILA1 in both leaves and leaf lamina joints. However,ila1 exhibits a visible phenotype mainly in its leaf lamina joints butnot in its leaves. <br />
<br />
===Evolution===<br />
MAPKKKs generally phosphorylate target proteins to mediate signal transduction.ILA1 Interacts with a Functionally Unidentified Protein Family. The kinase domain of ILA1 is involved in the interaction with IIPs in subfamily B, but not with IIPs in subfamily A. <br />
<br />
You can also add sub-section(s) at will.<br />
possible mechanism of ILA1 action<br />
Identification of ILA1 phosphorylation substrates is critical for understanding the signal transduction pathway of a Group C Raflike MAPKKK member. Through yeast two-hybrid screening of the cDNA library, six interaction proteins (IIPs) were found; these IIPs consist of a small family with unknown functions. The interactions were further confirmed by the BiFC and Co-IP analyses of a representative IIP. More importantly,ILA1 could phosphorylate an IIP in vitro. IIPs are thus likely to be the authentic downstream targets of ILA1. IIPs may act as transcription factors for they were regarded as putative transcription factors by bioinformatic analysis;they are nuclear localized in rice and they showed transactivation activity in yeast. <br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
# Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia <br />
# Department of Plant Biology, University of Georgia, Athens, Georgia <br />
# Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia<br />
<br />
==References==<br />
1.Jing Ning,Baocai Zhang,Nili Wang, Yihua Zhou, Lizhong Xiong.(2011).Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice.PLANT CELL.DOI:10.1105/tpc.111.093419<br />
2.Wang, L., Xie, W., Chen, Y., Tang, W., Yang, J., Ye, R., Liu, L., Lin, Y.,Xu, C., Xiao, J., and Zhang, Q.(2010). A dynamic gene expression atlas covering the entire life cycle of rice. Plant J.61:752–766.<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0286700&diff=173653Os06g02867002014-05-28T12:38:32Z<p>Matao152: /* Function */</p>
<hr />
<div>This is a broad-spectrum resistance(R) gene to rice blast<br />
==Annotated Information==<br />
尼松伟<br />
===Function===<br />
<br />
Blast is a very common disease found in rice. Blast causes immense damage to rice in rice-growing countries .And Pi9 alleles are mainly responsible for resistance to rice blast. We can use Pi1 and pi2 to improve the resistance ability to blast through hybridization.<br />
<br />
The rice disease blast, caused by the fungus Magnaporthe oryzae, is one of the most devastating diseases afflicting the rice crop <ref name="ref1" />and is best combated by the deployment of genetic resistance <ref name="ref2" />.<br />
<br />
===Expression===<br />
editing<br />
We test the relationship between Pi1,Pi2 and D12 gene in expression. When Pi1,Pi2 and D12 gene unit together, they will improve the resistance to blast by a great deal in the field condition.Pi9 gene lies on the chromosome6.<br />
<br />
===Evolution===<br />
The number of Pi2/9 family members in the four wild species ranges from two copies to 12 copies. Although these genes are conserved in structure and categorized into the same subfamily, sequence duplications and subsequent inversions or uneven crossing overs were observed, suggesting that the locus in diVerent wild species has undergone dynamic changes. <ref name="ref5" /><br />
<br />
[[File:Selection of the Pi9 Alleles.png]]<ref name="ref3" /><br />
<br />
To evaluate the phylogenetic relationship among the Pi9 alleles, we constructed neighbor-joining trees using the full-length fragment, the NBS region, and the LRR region. The analysis indicated that all these alleles are clustered into the same clade and belong to the same NBS-LRR sub-family. Two obvious haplotypes are distinguishable in the phylogenetic tree. Group I is mainly composed of the Asian cultivated rice O. sativa and its two ancestors, O. nivara, and O. rufipogon. Most of the African cultivated rice O. glaberrima and its ancestor O. barthii are clustered in group II. These results suggest that different selection pressures have occurred in the two unique groups of the Oryza species during domestication and/or natural selection.<ref name="ref3" /><br />
<br />
[[File:Genome organization of the Pi29 locus in four wild species.png]]<ref name="ref5" /><br />
<br />
The Pi2/9 gene family members are indicated by the arrows with the transcriptional direction. The family members within a same vertical group (VG) are indicated with the same color. The VG4 in which the three cloned resistance genes (Pi2, Pi9 and Piz-t) are located is highlighted by dashed lines in brown between diVerent species. The non-Pi2/9 homologues are indicated with rectangles. NIP nitrate-induced protein gene, PK protein kinase gene, UDP-T UDP-glycosyltrans-ferase-like protein, UTP UTP-glycosyltransferase-like protein. '''a''' The Pi2/9 locus in the cultivated rice line Nipponbare. Nbs3-OS-NPB was indicated in a Wlled gray arrow, which shows a signiWcant diVerence with other NBS-LRR genes as discussed previously <ref name="ref6" />, '''b''' The Pi2/9 locus in O. nivara, '''c''' The Pi2/9 locus in O. punctata, '''d''' the Pi2/9 locus in the BB subgenome of O. minuta, '''e''' The Pi2/9 locus in the CC subgenome of O. minuta, '''f''' the Pi2/9 locus in O. oYcinalis. The Wgure was drawn with the BioEdit program <ref name="ref7" />.<br />
<br />
== Nucleotide Polymorphism of the Pi9 Alleles ==<br />
<br />
[[File:Nucleotide polymorphism of the Pi9 alleles.png]]<ref name="ref3" /><br />
<br />
Of 2,927 nucleotides, 435 polymorphic sites including alignment gaps (∼18.9%) were detected among the 40 sequences using the DnaSP program. An intermediate-diversified nucleotide diversity (π=0.03348, P=0.000293) for the Pi9 alleles was observed based on the previously published criteria <ref name="ref4" />. The average nucleotide diversity of the LRR region (π=0.04554, P=0.00354; θ=0.04307, P=0.01263) is much higher than that of the NBS region (π=0.02098, P=0.00194; θ=0.02746, P=0.00816). Both values are similar in cultivated rice and wild rice groups. These results suggest that the LRR domain is important in the variation of the Pi9 alleles.<br />
<br />
<br />
== Pi2/9 locus ==<br />
Utilization of broad-spectrum resistance (R) genes is an effective and economical strategy to control the fungal pathogen Magnaporthe oryzae, the causal agent of the rice blast disease. Among the cloned blast resistance genes, Pi9, Pi2 and Piz-t confer broad-spectrum resistance to diverse M. oryzae isolates and were isolated from the Pi2/9 locus on chromosome 6. Identification and isolation of additional R genes with different resistance spectra from this locus will provide novel genetic resources for better control of this important rice disease.<ref name="ref8" /><br />
<br />
[[File:Genetic and physical maps around the Pi2-2 locus.png]]<ref name="ref8" /><br />
Genetic and physical maps around the Pi2-2 locus. (A) A high resolution map of Pi2-2. The numbers in parentheses above the map indicate the numbers of recombinants detected in the mapping population. (B) A virtual contig map spanning the Pi2-2 locus based on information of Nipponbare BACs released by RGRP (Rice Genome Research Program). (C) The position of the three NBS-LRR genes in the 270-kb contig. NIP, nitrite-induced protein. R1-R3, three putative NBS-LRR genes at Pi2/9 locus.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement,<br />
# National Center of Plant Gene Research and National<br />
# Center of Crop Molecular Breeding, Huazhong<br />
# National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan<br />
# National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan<br />
# Guangxi Key Laboratory of Subtropical Bioresource Conversation and Utilization,Nanning<br />
# Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong, China<br />
# Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences<br />
<br />
==References==<br />
1.H. L. Chen, B. T. Chen, D. P. Zhang, Y. F. Xie, and Qifa Zhang, 2001, Pathotypes of Pyricularia grisea in Rice Fields of Central and Southern China, Plant Disease 85:843-850.<br />
2 J. Liu : Y. Hu: Y. Ning : N. Jiang : J. Wu : Y. Xiao : X. Liu, L. Dai : G.-L. Wang2011, Genetic Variation and Evolution of the Pi9 Blast Resistance Locus in the AA Genome Oryza Species, J. Plant Biol. (2011) 54:294–302<br />
3. H. Jiang ,Y. Feng , L. Bao ,X. Li, G. Gao, Q. Zhang , J. Xiao , C. Xu _ Y. He,2012, Improving blast resistance of Jin 23B and its hybrid rice by marker-assisted gene pyramiding, Mol Breeding (2012) 30:1679–1688<br />
4. Zhao P，Feng R.R，Xiao Q Zh，Yang P.Zh，Lin. W，Liu P.Q，Li.RB,2013,Pyramiding brown planthopper genes, bph20（t）and bph21（t）,and rice blast resistant gene Pi9 in rice (Oryza sativa L.), Journal of Southen Agricalture，44（6）：885-892<br />
<br />
<references><br />
* <ref name="ref1">Ou SH (1985) Rice Disease, 2nd edn. Commonwealth MycologicalInstitute, Kew Surrey, pp 109–201</ref><br />
* <ref name="ref2">Bonman J, Khush G, Nelson R (1992) Breeding rice for resistance to pests. Annu Rev Phytopathol 30:507–528</ref><br />
* <ref name="ref3">J. Liu : Y. Hu: Y. Ning : N. Jiang : J. Wu : Y. Xiao : X. Liu, L. Dai : G.-L. Wang2011, Genetic Variation and Evolution of the Pi9 Blast Resistance Locus in the AA Genome Oryza Species, J. Plant Biol. (2011) 54:294–302</ref><br />
* <ref name="ref4">Yang S, Gu T, Pan C, Feng Z, Ding J, Hang Y, Chen J, Tian D (2008) Genetic variation of NBS-LRR class resistance genes in rice lines. Theor Appl Genet 16:165–177</ref><br />
* <ref name="ref5"> Liangying D, Jun W, Xunbo L, Xuejun W, Xionglun L, Chatchawan J, Dave K, Yeisoo Y, Rod A. W, Bin H, Bo Z, Guo-Liang W. Genomic structure and evolution of the Pi2/9 locus in wild rice species, Theoretical and Applied Genetics, 2010, 121(2): 295-309</ref><br />
* <ref name="ref6">Zhou B, Dolan M, Sakai H, Wang GL (2007) The genomic dynamics and evolutionary mechanism of the Pi2/9 locus in rice. Mol Plant Microbe Interact 20:63–71</ref><br />
* <ref name="ref7">Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Series 41:95–98</ref><br />
* <ref name="ref8">Nan Jiang;zhiqiang Li;Jun Wu;Yue Wang;Lijun Wu;Suhua Wang;Ting Wen;Yi Liang;Pinyong Sun;Jinling Liu;Liangying Dai;Zhilong Wang;Chao Wang;Meizhong Luo;Xionglun Liu;Guo-Liang Wang;Dan Wang. Molecular mapping of the Pi2/9 allelic gene Pi2-2 conferring broad-spectrum resistance to Magnaporthe oryzae in the rice cultivar Jefferson, Rice, 2012, 5: 29</ref><br />
</references><br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0286700|<br />
Description = Similar to NBS-LRR disease resistance protein homologue|<br />
Version = NM_001063943.1 GI:115467617 GeneID:4340778|<br />
Length = 2957 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0286700, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:10386510..10389466|<br />
CDS = 10386510..10386566,10386662..10389466|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:10386510..10389466<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:10386510..10389466<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atgcaggcattccttagagctgctgaagttatgaaaaagaaagatgaactattaaagttggtgaaacttagagagcgccaccggatcgctatccgtatccacaacctcaaatcaagagttgaagaagtgagtagcaggaacacacgctacaatttagtcgagcctatttcctccggcacagaggatgacatggattcctatgcagaagacattcgcaatcaatcagctcgaaatgtggatgaagctgagcttgttgggttttctgactccaagaaaaggctgcttgaaatgatcgataccaatgctaatgatggtccggccaaggtaatctgtgttgttgggatgggtggtttaggcaagacagctctttcgaggaagatctttgaaagcgaagaagacattaggaagaacttcccttgcaatgcttggattacagtgtcacaatcatttcacaggattgagctacttaaagatatgatacgccaacttcttggccccagttctctggatcaactcttgcaagaattgcaagggaaggtggtggtgcaagtacatcatctttctgagtacctgatagaagagctcaaggagaagaggtactttgttgttctagatgatctatggattttacatgattggaattggataaatgaaattgcatttcctaagaacaataagaagggcagtcgaatagtaataaccactcggaatgttgatcttgcggagaagtgtgccacagcctcactggtgtaccaccttgatttcttgcagatgaacgatgccataacattgctactgagaaaaacaaataaaaatcatgaagacatggaatcaaataaaaatatgcaaaagatggttgaacgaattgtaaataaatgtggtcgtctaccattagcaatacttacaataggagctgtgcttgcaactaaacaggtgtcagaatgggagaaattctatgaacaccttccttcagaactagaaataaacccaagcctggaagctttgaggagaatggtgaccctaggttacaaccacctaccatcccatctgaaaccatgctttttgtatctaagtatctttcctgaggattttgaaatcaaaaggaatcgtctagtaggtagatggatagcagaagggtttgttagaccaaaggttgggatgacgactaaggatgtcggagaaagttactttaatgagctaatcaaccgaagtatgattcaacgatcaagagtgggcatagcaggaaaaattaagacttgtcgaattcatgatatcatccgtgatatcacagtttcaatctcgagacaggaaaattttgtattgttaccaatgggagatggctctgatttagttcaggaaaacactcgccacatagcattccatgggagtatgtcctgcaaaacaggattggattggagcattattcgatcattagctatttttggtgacagacccaagagtctagcacatgcagtttgtccagatcaattgaggatgttacgggtcttggatcttgaagatgtgacattcttaatcactcaaaaagatttcgaccatattgcattgttgtgccacttgaaatacttgagtattggatattcgtcatccatatattcacttcccagatccattggtaaactacagggcctacaaactttgaacatgccgagcacatacattgcagcactaccaagtgagatcagtaaactccaatgtctgcatactcttcgttgtataggacagtttcattatgacaactttagtctaaaccacccaatgaagtgcataactaacacaatatgcctgcctaaagtattcacacctttagttagtcgcgatgatcgtgcaaaacaaattgctgaattgcacatggccaccaaaagttgctggtctgaatcattcggtgtgaaggtacccaaaggaataggtaagttgcgagacttacaggttctagagtatgtagatatcaggcggaccagtagtagagcaatcaaagagctggggcagttaagcaagctgaggaaattaggtgtgacaacaaacgggtcgacaaaggaaaaatgtaagatactttatgcagccattgagaagctctcttccctccaatctctccatgtggatgctgtgttattctcaggtattattggaacacttgagtgcctagattctatttcatctcctcctcccctactaaggacactcaggttgaatggaagtcttgaagagatgcctaactggattgagcagctcactcacctgaagaagttcgacttacggaggagtaaactaaaggaaggtaaaaccatgctgatacttggggcattgcccaacctcatggtcctttatctttatcggaatgcttaccttggggagaagctagtattcaaaacgggagcattcccaaatcttagaacactttgtatttacgaattggatcagctaagagagatcagatttgaggacggcagctcacccctgttggaaaagatagaaataggcaagtgcaggttggaatctgggattattggtatcattcaccttccaaagctcaaggagattccaattacatacggaagtaaagtggctgggcttggtcagctggagggagaagtgaacacacacccaaatcgccccgtgctgctaatgtacagtgaccgaaggtatcacgacctgggggctgaagccgaaggatcttctatagaagtgcaaacagcagatcctgttcctgatgccgaaggatcagtcactgtagcagtggaagcaacggatccccttcccgagcaggagggagagagctcgcagtcgcaggtgatcacgttgacgacgaatgataggtcagtcactccctacatggcagcttaa</cdnaseq>|<br />
AA = <aaseq>MQAFLRAAEVMKKKDELLKLVKLRERHRIAIRIHNLKSRVEEVS SRNTRYNLVEPISSGTEDDMDSYAEDIRNQSARNVDEAELVGFSDSKKRLLEMIDTNA NDGPAKVICVVGMGGLGKTALSRKIFESEEDIRKNFPCNAWITVSQSFHRIELLKDMI RQLLGPSSLDQLLQELQGKVVVQVHHLSEYLIEELKEKRYFVVLDDLWILHDWNWINE IAFPKNNKKGSRIVITTRNVDLAEKCATASLVYHLDFLQMNDAITLLLRKTNKNHEDM ESNKNMQKMVERIVNKCGRLPLAILTIGAVLATKQVSEWEKFYEHLPSELEINPSLEA LRRMVTLGYNHLPSHLKPCFLYLSIFPEDFEIKRNRLVGRWIAEGFVRPKVGMTTKDV GESYFNELINRSMIQRSRVGIAGKIKTCRIHDIIRDITVSISRQENFVLLPMGDGSDL VQENTRHIAFHGSMSCKTGLDWSIIRSLAIFGDRPKSLAHAVCPDQLRMLRVLDLEDV TFLITQKDFDHIALLCHLKYLSIGYSSSIYSLPRSIGKLQGLQTLNMPSTYIAALPSE ISKLQCLHTLRCIGQFHYDNFSLNHPMKCITNTICLPKVFTPLVSRDDRAKQIAELHM ATKSCWSESFGVKVPKGIGKLRDLQVLEYVDIRRTSSRAIKELGQLSKLRKLGVTTNG STKEKCKILYAAIEKLSSLQSLHVDAVLFSGIIGTLECLDSISSPPPLLRTLRLNGSL EEMPNWIEQLTHLKKFDLRRSKLKEGKTMLILGALPNLMVLYLYRNAYLGEKLVFKTG AFPNLRTLCIYELDQLREIRFEDGSSPLLEKIEIGKCRLESGIIGIIHLPKLKEIPIT YGSKVAGLGQLEGEVNTHPNRPVLLMYSDRRYHDLGAEAEGSSIEVQTADPVPDAEGS VTVAVEATDPLPEQEGESSQSQVITLTTNDRSVTPYMAA</aaseq>|<br />
DNA = <dnaseqindica>1..57#153..2957#atgcaggcattccttagagctgctgaagttatgaaaaagaaagatgaactattaaaggtttgggcagagcaaatacgtgacctgtcgtatgacattgaagattccctgatgaatttaaagtccatattgaaagccaaaccctatttcgtcagttggtgaaacttagagagcgccaccggatcgctatccgtatccacaacctcaaatcaagagttgaagaagtgagtagcaggaacacacgctacaatttagtcgagcctatttcctccggcacagaggatgacatggattcctatgcagaagacattcgcaatcaatcagctcgaaatgtggatgaagctgagcttgttgggttttctgactccaagaaaaggctgcttgaaatgatcgataccaatgctaatgatggtccggccaaggtaatctgtgttgttgggatgggtggtttaggcaagacagctctttcgaggaagatctttgaaagcgaagaagacattaggaagaacttcccttgcaatgcttggattacagtgtcacaatcatttcacaggattgagctacttaaagatatgatacgccaacttcttggccccagttctctggatcaactcttgcaagaattgcaagggaaggtggtggtgcaagtacatcatctttctgagtacctgatagaagagctcaaggagaagaggtactttgttgttctagatgatctatggattttacatgattggaattggataaatgaaattgcatttcctaagaacaataagaagggcagtcgaatagtaataaccactcggaatgttgatcttgcggagaagtgtgccacagcctcactggtgtaccaccttgatttcttgcagatgaacgatgccataacattgctactgagaaaaacaaataaaaatcatgaagacatggaatcaaataaaaatatgcaaaagatggttgaacgaattgtaaataaatgtggtcgtctaccattagcaatacttacaataggagctgtgcttgcaactaaacaggtgtcagaatgggagaaattctatgaacaccttccttcagaactagaaataaacccaagcctggaagctttgaggagaatggtgaccctaggttacaaccacctaccatcccatctgaaaccatgctttttgtatctaagtatctttcctgaggattttgaaatcaaaaggaatcgtctagtaggtagatggatagcagaagggtttgttagaccaaaggttgggatgacgactaaggatgtcggagaaagttactttaatgagctaatcaaccgaagtatgattcaacgatcaagagtgggcatagcaggaaaaattaagacttgtcgaattcatgatatcatccgtgatatcacagtttcaatctcgagacaggaaaattttgtattgttaccaatgggagatggctctgatttagttcaggaaaacactcgccacatagcattccatgggagtatgtcctgcaaaacaggattggattggagcattattcgatcattagctatttttggtgacagacccaagagtctagcacatgcagtttgtccagatcaattgaggatgttacgggtcttggatcttgaagatgtgacattcttaatcactcaaaaagatttcgaccatattgcattgttgtgccacttgaaatacttgagtattggatattcgtcatccatatattcacttcccagatccattggtaaactacagggcctacaaactttgaacatgccgagcacatacattgcagcactaccaagtgagatcagtaaactccaatgtctgcatactcttcgttgtataggacagtttcattatgacaactttagtctaaaccacccaatgaagtgcataactaacacaatatgcctgcctaaagtattcacacctttagttagtcgcgatgatcgtgcaaaacaaattgctgaattgcacatggccaccaaaagttgctggtctgaatcattcggtgtgaaggtacccaaaggaataggtaagttgcgagacttacaggttctagagtatgtagatatcaggcggaccagtagtagagcaatcaaagagctggggcagttaagcaagctgaggaaattaggtgtgacaacaaacgggtcgacaaaggaaaaatgtaagatactttatgcagccattgagaagctctcttccctccaatctctccatgtggatgctgtgttattctcaggtattattggaacacttgagtgcctagattctatttcatctcctcctcccctactaaggacactcaggttgaatggaagtcttgaagagatgcctaactggattgagcagctcactcacctgaagaagttcgacttacggaggagtaaactaaaggaaggtaaaaccatgctgatacttggggcattgcccaacctcatggtcctttatctttatcggaatgcttaccttggggagaagctagtattcaaaacgggagcattcccaaatcttagaacactttgtatttacgaattggatcagctaagagagatcagatttgaggacggcagctcacccctgttggaaaagatagaaataggcaagtgcaggttggaatctgggattattggtatcattcaccttccaaagctcaaggagattccaattacatacggaagtaaagtggctgggcttggtcagctggagggagaagtgaacacacacccaaatcgccccgtgctgctaatgtacagtgaccgaaggtatcacgacctgggggctgaagccgaaggatcttctatagaagtgcaaacagcagatcctgttcctgatgccgaaggatcagtcactgtagcagtggaagcaacggatccccttcccgagcaggagggagagagctcgcagtcgcaggtgatcacgttgacgacgaatgataggtcagtcactccctacatggcagcttaa</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063943.1 RefSeq:Os06g0286700]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=173626Os06g07249002014-05-28T11:28:53Z<p>Matao152: /* Labs working on this gene */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Please input function information here.<br />
<br />
===Expression===<br />
Please input expression information here.<br />
<br />
===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan , China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and<br />
Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
#<br />
<br />
==References==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan, China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and<br />
Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttttcttctctaatgatgatagtttatccaatttaatggtaaattttccaaaccatcgcacaactttctcaaaagaaattggagagctaagcaacagattgggatacatgtttttcacatctcagatctatgcaacgctttttatgtaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaaggtattattgattcattggctagtgattttctcttggaaatataagtttttgctgccaacatacttatgttttttttaacatactttactagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagaggtcaagagctaagctgaattttttttttcttgttactatttgacataacaaatgcagctccaatatcataatgtggacatcttttctttatgctttcctacttcataaaactacttatgttatagttcacattttttaaaagaggttatcttatggttcacatttcacaatctttcaatcaagtaaacaaacggttgacatggcagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggaggtaggcatctgtgacctatttaccatttcatgaaatcccaaattttcatattcttgcttgatggcctgatgggtgtatgtaacgtactgacagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattgatgaaattcttgtatcatatctaaagttgcacattgtttttctgcagctactttctcgatagatttctctgaaagatactgtgtattcaggggtttattattgtacagtgtggtagctgactggcagatcacttagccgccatttgtcttttccaccaaatgtaaagcaagtacccgtgtaattttcaattgctgtatagtgtatactacggaaagc</dnaseqindica>|<br />
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0724900&diff=173611Os06g07249002014-05-28T11:07:54Z<p>Matao152: /* References */</p>
<hr />
<div>Please input one-sentence summary here.<br />
<br />
==Annotated Information==<br />
===Function===<br />
Please input function information here.<br />
<br />
===Expression===<br />
Please input expression information here.<br />
<br />
===Evolution===<br />
Please input evolution information here.<br />
<br />
You can also add sub-section(s) at will.<br />
<br />
==Labs working on this gene==<br />
Please input related labs here.<br />
<br />
==References==<br />
# National Key Laboratory of Crop Genetic Improvement and National Center for Plant Gene Research (Wuhan), Huazhong<br />
Agricultural University, Wuhan, China<br />
# State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and<br />
Developmental Biology, Chinese Academy of Sciences, Beijing , China<br />
<br />
==Structured Information==<br />
{{JaponicaGene|<br />
GeneName = Os06g0724900|<br />
Description = Amino acid-binding ACT domain containing protein|<br />
Version = NM_001065152.1 GI:115470035 GeneID:4342105|<br />
Length = 5914 bp|<br />
Definition = Oryza sativa Japonica Group Os06g0724900, complete gene.|<br />
Source = Oryza sativa Japonica Group<br />
<br />
ORGANISM Oryza sativa Japonica Group<br />
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br />
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br />
clade; Ehrhartoideae; Oryzeae; Oryza.<br />
|<br />
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|<br />
AP = Chromosome 6:31694442..31700355|<br />
CDS = 31694488..31694678,31695034..31695148,31695248..31695333,31695455..31695565,31696057..31696111<br>,31696230..31696340,31697250..31697306,31697750..31697919,31698006..31698113<br>,31698546..31698619,31698724..31698897,31699089..31699186,31699405..31699485<br>,31699577..31699630,31699837..31699971,31700065..31700139|<br />
GCID = <gbrowseImage1><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage1>|<br />
GSID = <gbrowseImage2><br />
name=NC_008399:31694442..31700355<br />
source=RiceChromosome06<br />
preset=GeneLocation<br />
</gbrowseImage2>|<br />
CDNA = <cdnaseq>atggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagctgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaactgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggttattgagcataagccatatgattcaaaggctgatgtctttagctttggtgttgttttgtgggagctacttacgggaaagattcctcacgagtttctaacaccactccaagcagccataggtgttgttcaagagggtttaagaccagttattcccaaggccacagatcctaaactcgcgctgttacttgagagttgctggcagcaaaatgctgtcaacagaccagactttgtacaaattttgcaaaagcttgatgaaattgctggggagcatggcattgatcttacccatccccataaagagaaggagaaaggaggcttctttacttttgggaaggtccattga</cdnaseq>|<br />
AA = <aaseq>MDHGGQVSPVPATATAPRKVRREHMRLGVYHDVLQRLRDAGAPE ALAPDFAEKLWTHFHRFNASYAMDVNVERAEDVLMHMKLLEKAIHPENQPAFSVRIVQ VPLDIDASEADSQSNITEDDNCPTPRTPAEHPAPIFGSTTALKALVRQASSKNLLDDN QDIDAILRPMHEITFASDDKPKGLTQLSSLLGNLNLDIKEVHALSTNDGYFLDIFIVI GWDHKETQLLEEALEKEIHNYEPQMPSKSSCWPPELAGKQSLINSQVNHVQIPKDNTD EWEINFDVLDIQEKVASGTYGDLYRGTYFGEDVAIKVLKSDRLNENMQEEFNEEVFIM RKIRHKNIVRFLGACTKSPTLCIVTEFMKNGSVYDYLHKRKGSFKLPSLLKAAVDISK GMNYLHQNKIIHRDLKTANLLMDEHELIKVADFGVARVKAESGIMTAETGTYRWMAPE VIEHKPYDSKADVFSFGVVLWELLTGKIPHEFLTPLQAAIGVVQEGLRPVIPKATDPK LALLLESCWQQNAVNRPDFVQILQKLDEIAGEHGIDLTHPHKEKEKGGFFTFGKVH</aaseq>|<br />
DNA = <dnaseqindica>47..237#593..707#807..892#1014..1124#1616..1670#1789..1899#2809..2865#3309..3478#3565..3672#4105..4178#4283..4456#4648..4745#4964..5044#5136..5189#5396..5530#5624..5698#tgcagcgtttgcagtcgttgtgcgcatttcgtcgaacaggtcggcgatggaccacggcggtcaggtctctcccgtgccggcgacggcgacggcgccgcggaaggtcaggagagaacacatgaggctgggggtgtaccacgacgtgctgcagcggctcagggacgccggcgcgcccgaggccctcgcgccggacttcgccgagaagctctggacgcacttccaccgcttcaacgccaggttcttaatacaaccaccctgcgcatcattgctgccaaaaactcgtgtctgcatgtttgatttcgtctctaagattgatctttgatcgaatgcatacggggcactgttcaaactaaaatttaccctctgtgatgattttcaagtgtcaggcctgtgaatccgaatcagggcctgaaatggaagatgaatttggctaacatttctgagtctaagcacactaggagttggctagagtctaaatttcgatgctctactttttgatatgggggcgctcccagtgcctaatttgttcttttcgaattgctggttccaaatatgatcaactctattcttgttcttcgatttgatatggcagttatgccatggatgtcaatgttgagagggcagaagacgttctgatgcacatgaagctgcttgagaaggctatccatcctgaaaatcagcctgccttctcagtgagaattgttcaggtacatttacgccaaaaaggagaacagcttcgattggtttgctcatgttttgcagcagtatatctgtggtatgtttctgaagcaaacattgctgtgcaggttcctctagatattgatgcgagtgaagccgattcacagtcaaacatcactgaagatgacaactgtcctacgccaagaacaccagcgtatgtcctttaagattgctatgtatcatgacatgttgttatatttttctatttgtgcttagtgctagtatttatattctgttttttctccccttatatttctgtttgtttgatttctcagtgaacaccctgcacctatttttggctctaccacagcccttaaggctcttgttcgtcaagcaagcagcaagaacctcctggatgacaaccaagacatagatgctattctcaggtttgcatatttatagttactaggtcctgaaattttctcatttacctatgcacttttttctctttgcactttcggatttttctgttttagacattcatttttaattcaaatatcactaattagctcattttgctgttacttgatgttgtgtttattcctttttttcagtcagagtgtggttatttctttaaaacactaatcagtgagactatgatatttagaaccatttcttctgtttcattgctgtagttctatttaacatgcacatatgttgaagactgtttatgtcagtacatggtattcttctttcatttatcgtctttgtactctgagatttgggaataagtgagtgcaattccacaatctatgcttctctcttttcaaaaaaaaaaaaacattctatgcatctgaataaaaagctggggaggagcataacatgatgcttattcgtttctctcttttcccttggctgcaaatttcttatcttgcagacccatgcatgagatcacctttgcatctgatgacaaaccaaagggccttactcaagtaagttttcttactccaaacttctggaatagcaaaaccgaggattcattactatggaagaatttgttacacatgttttgagagccttcctgatactatcggttgcatattatttcagctgtcttcacttctaggcaatctcaatcttgatatcaaagaagtacatgcactttcaacaaatgatggttacttcttagatattttcatcgtaatcggatgggatcacaaggtattgtttggtccatttctctactcaggaaacatttgattaaaaattcttaaccatattctccctctatcccaaaatataagcactcaggtgcttttcacgaggatcaaggagagacatgaaattaaatgggggatatttgtcactggttgagatgtggaagtaagtagagaaacaaaataagagatggttgtgattggttgagatgagggaataggtggagaagtagctatattttaggacaaaatttgaatgctcaaagtagctctattttgggatggagggagtagttgtctgtcttgcttcctacatgtaaggacatctaagtattcatgatctcaacattcattcatacttttaccattgttttgcatgaattgctatgaatccaactgagtttatatatattctatgacctttgatctactaattttctagtaaaattttatagcacaatgtccataatataggattgtctaagaccatatcccaaaccagttttgaaccagtacatcttatctatgctttgtaatcccaaagcatttgcctaaatgccatttagagtttatttgcctgttcatgaagagctcaagaaaactctgagaaaaaaatgttatttgactactgttaatggttatcagtgaacttataggcacgtagttgcatattattggattgcacaggaatattagaaacagcaaaaaatgatttttgcatattttgggctgacaaaccggaaagctttattgttccctttttagctaccttaaacaattattttgtgaaaatcagccgaaaaattggtgggagttctgttactgtaattttaaattcgcaaggatctgttccctttgctgaataagaatatgcttgttagaaaggaggataattattcttttgattttacaggaaacccaacttctcgaagaagcgttggagaaggaaattcacaattacgaaccacaggtagagtttcattgatattcctctttattttctcactataattaaaatgcattagagctttgtttgctctgttgccagaaatgtttaaaaaaatatgcagaaactagataaaatatagtgctgaagtcaaccgaaaacaaaataaaagaagtttaagattttcctgcccaccatacgatagtactgcacaatcttaaaggtcagtagtttacatcacaagctcttgaaggcgcataagaaaagggggattcagaagtattggaatagtatgctttgttctagatcaagaagatatggttacatcatagaatatgatgttctcttgacaaataacaattcagttcgtttattaagctattgtatttctctctctgccaatactgattacagcatttctatatttcctaatacttactgttttcattgttgaaaattttctgcagatgccctcaaaatcttcttgctggcctcctgaattagcaggcaagcagtctttgattaactcgcaagtcaaccatgttcagatacctaaagataacacagatgaatgggaaattaacttcgacgtattggatattcaagagaaagtggcatctggaacgtatggtgatctgtgagtatcctgttgttgcgttgcatggaaccttcagtctgttagacattgcctcaagagtcattcttcctcccttgaggttgcagctatcgcggtacatattttggtgaagatgtcgcaattaaggtgctcaagtcagatcgtcttaatgagaacatgcaggaggaatttaacgaagaagtattcatcatgaggttagtaataaaataaagatatgggattctcaaaaccaaaaaaaataaaaaggaaaagtaaataaacctgtaatgtcgaatgcctagcaagctcatctagtaactaagccatcgctgtataaggtgtagcgagatgactttcccaatggcttcgagtcattcattgaaatgtacaagtcgtttatgaacacatcatgtgaatcatattatgcaaagttttgtctgaaccatagaaaaacttgcacaaacttaatcatagcatacagcacaatctaagaatttgacaacattacagctatacaaccttaagtaattcagcagtctatttggtaagctgattgaagttacatagacaattctttatcagagccttttccctggaaagtaacacatctctttgaccatgttgccgatggaactgccaaattctaggaagattcgtcataagaacattgttcgatttcttggggcatgcaccaaatccccaaccttatgtattgtcacaggtaaaatgaatacagatgccaattccttttaattatgcagattttgcccatttatatgtgatggtgttatccccttacattttggaatttacatatttcaccagagtttatgaaaaatggaagtgtttacgactaccttcataagcgtaaaggttccttcaaacttcccagtctgcttaaagctgcagttgatatatcaaaagggatgaactatttacaccaaaataaaattattcatcgagacttgaagacagcaaaccttcttatggatgagcacgaggcaagacttttgatcgctctgtagcttaatcctgagtagtcattaacgtaattactttataatgtggcttgatgatcagggataaacatagtttttcatggcctgtagtttgttggtattgctttctttgcagaactgttgttgtcacaattattttcatgattaaactgatatggatgaatctttttcagcttatcaaggttgctgattttggtgttgcacgtgtaaaagctgaatcagggattatgactgccgaaactggtacatatcgttggatggctcctgaggtaatctttttaaattatatattcaagtgaaatcccagttttgcatagaggacatagcaaatgtttcccttt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/>
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065152.1 RefSeq:Os06g0724900]|<br />
}}<br />
[[Category:Genes]]<br />
[[Category:Japonica mRNA]]<br />
[[Category:Oryza Sativa Japonica Group]]<br />
[[Category:Japonica Genes]]<br />
[[Category:Japonica Chromosome 6]]<br />
[[Category:Chromosome 6]]</div>Matao152