http://192.168.164.12:81/ricewiki/api.php?action=feedcontributions&feedformat=atom&user=Mayamei0424 2026-05-27T11:26:52Z User contributions MediaWiki 1.30.0 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0235200&diff=178386 2014-06-05T12:38:43Z

<p>Mayamei0424: /* Function */</p> <hr /> <div>''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family,and regulates panicle development and seed size in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family. ''SP1'' is preferentially expressed in young panicle，especially in the phloem of the branches of young panicle.''SP1'' regulates panicle development and seed size in rice.<br /> <br /> ===Mutation===<br /> [[File:SP1图1.png|right|thumb|250px|Figure1. Phenotype of the ''sp1-2'' mutant. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> ''sp1-2'' is one TOS17 insertion mutant. &lt;br&gt;<br /> '''(1) Phenotypic comparison between wild-type and ''sp1-2'' plants after heading.''' &lt;br&gt;<br /> The ''sp1-2'' mutant plant showed a slight reduction in plant height, owing to the shorted panicle (Figure 1a). &lt;br&gt;<br /> '''(2) Comparison of panicle sizes between the wild-type and ''sp1-2'' plants at the grain filling stage.''' &lt;br&gt;<br /> In comparison with the wild type, the mature panicle length of ''sp1-2'' was strikingly reduced (Figure 1b). &lt;br&gt;<br /> '''(3) Comparison of mature grains size between the wild type and ''sp1-2''.''' &lt;br&gt;<br /> The seed size of the ''sp1-2'' mutant was smaller than the wild type (Figure 1c). &lt;br&gt;<br /> '''(4) Comparison of mature grains weight between the wild type and ''sp1-2''.'''&lt;br&gt;<br /> In consisted with the smaller seeds, the weight of 1000 seeds was lower of the ''sp1-2'' mutant compared to the wild type (Figure 1d). &lt;br&gt;<br /> These results indicated that the ''SP1'' affects not only the panicle size, but also the grain size.<br /> <br /> ===Cloning of the ''SP1'' gene===<br /> [[File:SP1图2.png|right|thumb|250pxFigure 2. Map-based cloning and confirmation of the ''SP1'' gene. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> Map-based approach was used to clone the ''SP1'' gene. Primary gene mapping showed that the ''SP1''locus is located between the molecular markers S21074 and RM3701 on chromosome 11 (Figure 2a), and then fine mapping narrowed the locus to an 8-kb region between markers M7 and M8 (Figure 2b). Annotation of the 8-kb sequence identified an open reading frame (ORF), LOC_Os11g12740, which is composed of five exons and four introns (Figure 2c). DNA sequence comparison revealed a 31-bp deletion in the third exon in ''sp1-2''. The identity of ''SP1'' is further confirmed by genetic complementation. The plasmid pSPC containing the entire LOC_Os11g12740 ORF and pSPCT containing the partial coding region of the ORF were introduced into the ''sp1-2'' mutant (Figure 2d). All the 10 transgenic lines of pSPC showed a complementation of the sp1-2 phenotype, whereas all of the eight lines of pSPCT failed to rescue the ''sp1-2'' phenotype (Figure 2e,f). RT-PCR analysis showed that the transcription level of the ''SP1'' gene is dramatically decreased in young panicles of ''sp1-2'' (Figure 2g). These results presented above showed that the rice ''SP1'' gene and its mutation is responsible for the altered phenotype of sp1.<br /> <br /> ===Expression===<br /> [[File:SP1图3.png|right|thumb|250px|Figure 3. Expression pattern of ''SP1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> The spatial and temporal expression pattern of ''SP1'' is analyzed by GUS reporter system and RT-PCR.&lt;br&gt; <br /> As shown in figure 3a-m, weak GUS signals could be detected in panicle axes upon the formation of spikelets (Figure 3a), subsequently, the GUS signals become stronger in rachis, branches, stigmas, ovules, and the surfaces of palea and lemma during the elongation stage of the young panicle (Figure 3b-g). The GUS signals are barely detectable in mature panicle (Figure 3h). And the GUS signals are hardly detected in root, culm and leaf (Figure 3i-k). Interesting, the SP1-GUS fusion protein is specially located in the phloem of vascular bundles of panicle rachis (Figure 3l-m). RT-PCR analysis results indicated that ''SP1'' highly expressed in young panicle (Figure 3n).<br /> <br /> ===Evolution===<br /> [[File:SP1图4.png|left|thumb|250px|Figure 4. Phylogentic analysis of the ''SP1'' amino acid sequence with plant peptide transporter (PTR) proteins. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> Bioinformatic analysis revealed that the deduced amino acid sequence of ''SP1'' contains a conserved PTR2 domain, implying that ''SP1'' may encode a PTR family protein. Phylogenetic analysis showed that the ''SP1'' gene is classified into a distinct clade for nitrate transporters, and is closest to AtNRT1:2, an Arabidopsis nitrate transporter (Figure 4). &lt;br&gt;<br /> (Footnote: In Figure 4,the amino acid sequences of the entire proteins were aligned by CLUSTALX, and the phylogenetic tree was constructed using the neighbor-joining algorithm. Bootstrap values are shown at each node. The circle and the triangle indicate peptide and nitrate transporters, respectively. The square indicates a dicarboxylate transporter.)<br /> <br /> ===Knowledge Expansion===<br /> [[File:SP1图5.png|right|thumb|250px|Figure 5. ''SP1'' sequences and the predicted hydropathy profile of ''SP1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:SP1图6.png|left|thumb|250px|Figure 6. Subcellular localization of the SP1-GFP fusion protein. .(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> Most members of the PTR family contain 12 a-helical transmembrane domains, and are classified into four groups according to their structure &lt;ref name=&quot;ref2&quot;/&gt;. Hydropathy analysis shows that SP1 protein indeed contained 12 transmembrane domains (TMDs), harboring a long hydrophilic loop between TM6 and TM7 (Figure 5b,c). Three consensus sequences, GxxIADxWLGxFx TIxxxxxVxxxG, LGTGGIKPxV and FxxFYLxINxGSL, have been characterized for PTR proteins. However, these motifs were not strictly conserved in all PTR proteins &lt;ref name=&quot;ref3&quot;/&gt;&lt;ref name=&quot;ref4&quot;/&gt;. This is also the case in ''SP1'', in which there are several alternatives within the conserved motifs (GxxLSDxYLGxFxTMxxxxxVxxxG, LGSGCLKPxI and FxxAYFxFCxGEL). In consistent with the 12 TMDs, the ''SP1'' is located at the plasma membrane ,which is correlates with the ''SP1'' action ,as one nitrate transporter(figure 6). In contrast with the control, signal is observed ubiquitously in onion epidermal cells, in which the SP1-GFP fusion protein is exclusively localized in the plasma membrane (Figure 6a–d). And analysis of ACTIN:SP1-GFP transgenic rice also indicates that ''SP1'' localizes at the plasma membrane in rice (Figure 6e–f).&lt;br&gt;<br /> (Footnote: In Figure 5,(a) The nucleotide sequence and the deduced amino acid sequences of ''SP1''. Numbers at left refer to the positions of nucleic acids. The box highlights the 31-bp deletion in sp1-2. The asterisk indicates the stop codon introduced by the 31-bp deletion. The shaded letters refer to the conserved PTR2 domains, and the underlined letters indicate the 12 transmembrane domains. (b) Prediction of membrane-spanning regions and their orientation of ''SP1'' made by the TMPRED program. (c) A prediction model for the transmembrane topology of ''SP1''.)<br /> <br /> ==Labs working on this gene==<br /> *State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China,<br /> *State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences,Hangzhou 310006, China,<br /> *Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8652, Japan,<br /> *Research Institute for Bioresources, Okayama University, 2-20-1, Chuo, Kurashiki, Okayama 710-0046, Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Shengben Li, Qian Qian, Zhiming Fu, Dali Zeng, et al. (2009) ''Short panicle1'' encodes a putative PTR family transporternand determines rice panicle size.The Plant Journal . 58, 592–605.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Chiang, C.S., Stacey, G. and Tsay, Y.F. (2004) Mechanisms and functional properties of two peptide transporters, AtPTR2 and fPTR2. J. Biol. Chem. 279, 30150–30157.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref3&quot;&gt;Fei, Y.J., Ganapathy, V. and Leibach, F.H. (1998) Molecular and structural features of the proton-coupled oligopeptide transporter superfamily. Prog. Nucleic Acid Res. Mol. Biol. 58, 239–261.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref4&quot;&gt;Stacey, G., Koh, S., Granger, C. and Becker, J.M. (2002) Peptide transport in plants. Trends Plant Sci. 7, 257–263.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os11g0235200|<br /> Description = TGF-beta receptor, type I/II extracellular region family protein|<br /> Version = NM_001074096.1 GI:115484840 GeneID:4350145|<br /> Length = 5257 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0235200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:7176158..7181414|<br /> CDS = 7176608..7177532,7177841..7177965,7179701..7180180,7180759..7180976,7181105..7181243&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;gctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;ANMDVESRQGPKGGDTMAASEVSVDWRGRPCGSRKHGGMKAAVF VLGIQAFEMMAIAAVGNNLITYVFNEMHYPLSKSANIVTNFIGTVFLLSLLGGFLSDS YLGSFWTMLIFGFVELSGFILLAVQAHLPQLRPPACDMMAAAAAEGGCEEASGVKAGI FFAALYLVALGSGCLKPNIIAHGADQFRRGGGGGGDGDGDDGGDGKRLSSYFNAAYFS FCVGELVALTVLVWVQTRSGMDVGFGVSAAAMAVGLVSLVAGVFFYRNKPPQGSIFMP IAKVFVAAVTKRKQVCPSSSSTTAASHAVIPATGAAPVHRINKFRFLDKACVKVQDGH GGGGDGGKESVWPPCTPAEVEQVKVLLCVVPIFACTIVFNTILAQLQTFSVQQGSAMD TRIGGGGGGAAFHIPPASLQAIPYLVLIALVPAYEACFVPAMRRATGVDTGITPLQRI GVGLFAVTFSMVAAALVEAHRRRHAGDGLLSIFWIAPQFLVFGVSEMFTAVGLIEFFY KQSLSGMQAFLTSMTYCSYSFGFYLSSLLVSLVNKVTSGDGAGAGGGGWLSDNDLNKD RLDLFYWLLAGLSLLNFFNYLFWSRWYSKSVETTVQVAGVGGEGGGGEQQEEKGVDEV NMGH&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;3883..4807#3450..3574#1235..1714#439..656#172..310#gcctgaccacacaagcacaaagcaagcactatcaccaaaaggatcagagaagaaactgatatcactatctgatcagtgtagcattgcagcctctcttcttggtaggagtgagctagcacagtgaagagctaattaagccatttgtgtgagcttgcgttgagtgagctctgagctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctaggttcgtttaattagttagttactctcttaatccattatttcttgggtagaaatatgttgcttgatctgatttgttcttgtttgtgtttgtgttttgttggatatcgtttgcattgatgatgattgcagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgtaagaaattgattcatccatccttcatcgtcgatctcttctctttgatatcgcactacgataaaattgacattctttacacctagatgcaactttttgggtccttttttagactgcatttcttggctccttgcaagcaagaacagctgaacaggatcggatcttgtttgtttgtttgtttaatcatgtagggatgttgtttctaggtaggattatatataaaattaaccagataagacacgcgaaagattcagcgactgttcatcgtgccgcgaaaatgatgaggttttgaggaggaggcagcaagttcagcgtaaaggtgagagagagagtggaagaaaatcatggctgcctccatccaatgcggccatgctagctagtgcgccgcccaaaacgccactcgactccacaatatcatttcttggcttttgattttaattttcgagcaggttcattgcatcccatcccatgaccatatccatggcgataattaaccattactgattaatgcgccgccaactttagttagtttagctaataatttttgttgtttttttatttctaatttttcttggcaggggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggtacgtacgttatgccgtacaatacaatcgtcgtacgtgtacggttcgtcatgtacttttatacgctttcttcttttttttttttagagaaaaatatttcttacccggcctctacatccaatcagatatatgcagactttttaaattagaaacttaactctcaaacaatctaatctgaaacttgctactacgattaaactctagatctttaggatacgcttaggacattgcaaccacaattgcatcttcttttcttttctagttggcacattgaaatatcttttgatataattttgacagccatcttttttttaaatgaactctctgtttctaaaatataataacatagtattccattccatgtaacctagtactacgaatctatgtttaattgaactatgaatttagtagatttatagtactgtgttggtgtctattctagtactacgaatttatctcattctgttttagcatcttatattttaggagaggtaggattatttttaatcggacggagtatacaagttggaaacaaatactacatcattgtgttgcatttgcaaaagcagagccaaccaactctgtttttgtgccagctactatattcccctaaaaatattgttgcattcataaatattggaggtcaattttaagctaggtctatgttctctgttcctgcatatggtagttacttgaaaaatcctgaaaatatgataacgtgcattatggtgccatctgtcgctacacaaatatatagtaatatataagtataaaggttagagaaaacatggccgtgggccctagcaatgcgtttctgcactagctaaatagaatatatataatatgtgacaagactaattaatattaagaagcaccataggtagatcaaatttaaaaatgtaaggatgaaaaggcatgtgtatgtacaacaaattaaacaacagaaagtttgaccatacgtatcatcctgattccttgtttggaaagtactactccagatacgctgcttttatatactagtataaaagcaaaaccgcctattttcgttaaatataatgcataatctttatttgatccaaaaaagctacatgatcaaacaggagtttggtacagggactattttatcctcatagtcacctcgtgcactaccacataaaaagcacacgagatcatgggtttacaaccgtttctacattaaacaaaatgaaatgctacctagtttattgccaaatgaagtatttagtcaataaaggaaaagaaagcatatatatgatttactgaatgagataataatgtatatgtatgttgggataaaaaaaataaaactgatgattttaagttacacactggctggcctcctctgccttttctttcttctttctgggacaagttgacaagggctagtggctcagctttatttctctcttttgtgtgggtcaagaattaccactgtctgctgggaggtacctttgattattgatcacagaaatggaggcccattctgttatgcttgtgatacatcaatcatgtttgcatgtgtggggagagacatgtaacatgactgacaatgatctcaggacagcatatatatctttccagcttaattttgtttttagttcttgacttagatcggtataaaccccctaaaactcaattgcacgtgaaaataacaaactctcactcgatcgctgacattttgagctgatggtcggtacatgtgactcaatatgtataggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggtaggtaaaaaaatatttgtaagtggcactgtaatatactacttatttttaaaaaatatatggcaccgttaacttttcgcataatgtttgaccattcgtcttattcaaaaatttaatattaattcataaaaatataagttaagattatattttatttgataataaaacaagtcacaacaagataaaaataaaatttatataatttttttaataagattaatagataaatattatgccaaaagtcaaatggcgtgatatatttcgaaggagtataatagtttactcatttcatgtatgcatgcttacaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactgacatgtgggttcgaaccgcacgtctagtatattaggtggattattaatcatttaacctagtcttaaccttttttttatggtttttccttgtatggaaaaatattagtaagggtggatgtatatgagtccccacctaattaaggagagtgtgtgtgatgtataaattaattaatcaaaagggattaggtgattattacttagtaatgatccctgattaggtcaagtcaaaaggtagtagttctgagtttgactctgcaaggatgcaagagtagtggtatttgggtagggatcaagtgatcaagtacgtatctagtccttttctttaatgataaggagctagtatatatctatgtactttatatatgtagctatcatgtaaatgacaaaaggatttgccataaaagcaaagtactgataatagttggtgcaaaaaatactttcactgttgatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074096.1 RefSeq:Os11g0235200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0235200&diff=178382 2014-06-05T12:37:21Z

<p>Mayamei0424: /* References */</p> <hr /> <div>''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family,and regulates panicle development and seed size in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family. SP1 is preferentially expressed in young panicle，especially in the phloem of the branches of young panicle.SP1 regulates panicle development and seed size in rice.<br /> <br /> ===Mutation===<br /> [[File:SP1图1.png|right|thumb|250px|Figure1. Phenotype of the ''sp1-2'' mutant. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> ''sp1-2'' is one TOS17 insertion mutant. &lt;br&gt;<br /> '''(1) Phenotypic comparison between wild-type and ''sp1-2'' plants after heading.''' &lt;br&gt;<br /> The ''sp1-2'' mutant plant showed a slight reduction in plant height, owing to the shorted panicle (Figure 1a). &lt;br&gt;<br /> '''(2) Comparison of panicle sizes between the wild-type and ''sp1-2'' plants at the grain filling stage.''' &lt;br&gt;<br /> In comparison with the wild type, the mature panicle length of ''sp1-2'' was strikingly reduced (Figure 1b). &lt;br&gt;<br /> '''(3) Comparison of mature grains size between the wild type and ''sp1-2''.''' &lt;br&gt;<br /> The seed size of the ''sp1-2'' mutant was smaller than the wild type (Figure 1c). &lt;br&gt;<br /> '''(4) Comparison of mature grains weight between the wild type and ''sp1-2''.'''&lt;br&gt;<br /> In consisted with the smaller seeds, the weight of 1000 seeds was lower of the ''sp1-2'' mutant compared to the wild type (Figure 1d). &lt;br&gt;<br /> These results indicated that the ''SP1'' affects not only the panicle size, but also the grain size.<br /> <br /> ===Cloning of the ''SP1'' gene===<br /> [[File:SP1图2.png|right|thumb|250pxFigure 2. Map-based cloning and confirmation of the ''SP1'' gene. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> Map-based approach was used to clone the ''SP1'' gene. Primary gene mapping showed that the ''SP1''locus is located between the molecular markers S21074 and RM3701 on chromosome 11 (Figure 2a), and then fine mapping narrowed the locus to an 8-kb region between markers M7 and M8 (Figure 2b). Annotation of the 8-kb sequence identified an open reading frame (ORF), LOC_Os11g12740, which is composed of five exons and four introns (Figure 2c). DNA sequence comparison revealed a 31-bp deletion in the third exon in ''sp1-2''. The identity of ''SP1'' is further confirmed by genetic complementation. The plasmid pSPC containing the entire LOC_Os11g12740 ORF and pSPCT containing the partial coding region of the ORF were introduced into the ''sp1-2'' mutant (Figure 2d). All the 10 transgenic lines of pSPC showed a complementation of the sp1-2 phenotype, whereas all of the eight lines of pSPCT failed to rescue the ''sp1-2'' phenotype (Figure 2e,f). RT-PCR analysis showed that the transcription level of the ''SP1'' gene is dramatically decreased in young panicles of ''sp1-2'' (Figure 2g). These results presented above showed that the rice ''SP1'' gene and its mutation is responsible for the altered phenotype of sp1.<br /> <br /> ===Expression===<br /> [[File:SP1图3.png|right|thumb|250px|Figure 3. Expression pattern of ''SP1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> The spatial and temporal expression pattern of ''SP1'' is analyzed by GUS reporter system and RT-PCR.&lt;br&gt; <br /> As shown in figure 3a-m, weak GUS signals could be detected in panicle axes upon the formation of spikelets (Figure 3a), subsequently, the GUS signals become stronger in rachis, branches, stigmas, ovules, and the surfaces of palea and lemma during the elongation stage of the young panicle (Figure 3b-g). The GUS signals are barely detectable in mature panicle (Figure 3h). And the GUS signals are hardly detected in root, culm and leaf (Figure 3i-k). Interesting, the SP1-GUS fusion protein is specially located in the phloem of vascular bundles of panicle rachis (Figure 3l-m). RT-PCR analysis results indicated that ''SP1'' highly expressed in young panicle (Figure 3n).<br /> <br /> ===Evolution===<br /> [[File:SP1图4.png|left|thumb|250px|Figure 4. Phylogentic analysis of the ''SP1'' amino acid sequence with plant peptide transporter (PTR) proteins. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> Bioinformatic analysis revealed that the deduced amino acid sequence of ''SP1'' contains a conserved PTR2 domain, implying that ''SP1'' may encode a PTR family protein. Phylogenetic analysis showed that the ''SP1'' gene is classified into a distinct clade for nitrate transporters, and is closest to AtNRT1:2, an Arabidopsis nitrate transporter (Figure 4). &lt;br&gt;<br /> (Footnote: In Figure 4,the amino acid sequences of the entire proteins were aligned by CLUSTALX, and the phylogenetic tree was constructed using the neighbor-joining algorithm. Bootstrap values are shown at each node. The circle and the triangle indicate peptide and nitrate transporters, respectively. The square indicates a dicarboxylate transporter.)<br /> <br /> ===Knowledge Expansion===<br /> [[File:SP1图5.png|right|thumb|250px|Figure 5. ''SP1'' sequences and the predicted hydropathy profile of ''SP1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:SP1图6.png|left|thumb|250px|Figure 6. Subcellular localization of the SP1-GFP fusion protein. .(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> Most members of the PTR family contain 12 a-helical transmembrane domains, and are classified into four groups according to their structure &lt;ref name=&quot;ref2&quot;/&gt;. Hydropathy analysis shows that SP1 protein indeed contained 12 transmembrane domains (TMDs), harboring a long hydrophilic loop between TM6 and TM7 (Figure 5b,c). Three consensus sequences, GxxIADxWLGxFx TIxxxxxVxxxG, LGTGGIKPxV and FxxFYLxINxGSL, have been characterized for PTR proteins. However, these motifs were not strictly conserved in all PTR proteins &lt;ref name=&quot;ref3&quot;/&gt;&lt;ref name=&quot;ref4&quot;/&gt;. This is also the case in ''SP1'', in which there are several alternatives within the conserved motifs (GxxLSDxYLGxFxTMxxxxxVxxxG, LGSGCLKPxI and FxxAYFxFCxGEL). In consistent with the 12 TMDs, the ''SP1'' is located at the plasma membrane ,which is correlates with the ''SP1'' action ,as one nitrate transporter(figure 6). In contrast with the control, signal is observed ubiquitously in onion epidermal cells, in which the SP1-GFP fusion protein is exclusively localized in the plasma membrane (Figure 6a–d). And analysis of ACTIN:SP1-GFP transgenic rice also indicates that ''SP1'' localizes at the plasma membrane in rice (Figure 6e–f).&lt;br&gt;<br /> (Footnote: In Figure 5,(a) The nucleotide sequence and the deduced amino acid sequences of ''SP1''. Numbers at left refer to the positions of nucleic acids. The box highlights the 31-bp deletion in sp1-2. The asterisk indicates the stop codon introduced by the 31-bp deletion. The shaded letters refer to the conserved PTR2 domains, and the underlined letters indicate the 12 transmembrane domains. (b) Prediction of membrane-spanning regions and their orientation of ''SP1'' made by the TMPRED program. (c) A prediction model for the transmembrane topology of ''SP1''.)<br /> <br /> ==Labs working on this gene==<br /> *State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China,<br /> *State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences,Hangzhou 310006, China,<br /> *Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8652, Japan,<br /> *Research Institute for Bioresources, Okayama University, 2-20-1, Chuo, Kurashiki, Okayama 710-0046, Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Shengben Li, Qian Qian, Zhiming Fu, Dali Zeng, et al. (2009) ''Short panicle1'' encodes a putative PTR family transporternand determines rice panicle size.The Plant Journal . 58, 592–605.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Chiang, C.S., Stacey, G. and Tsay, Y.F. (2004) Mechanisms and functional properties of two peptide transporters, AtPTR2 and fPTR2. J. Biol. Chem. 279, 30150–30157.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref3&quot;&gt;Fei, Y.J., Ganapathy, V. and Leibach, F.H. (1998) Molecular and structural features of the proton-coupled oligopeptide transporter superfamily. Prog. Nucleic Acid Res. Mol. Biol. 58, 239–261.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref4&quot;&gt;Stacey, G., Koh, S., Granger, C. and Becker, J.M. (2002) Peptide transport in plants. Trends Plant Sci. 7, 257–263.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os11g0235200|<br /> Description = TGF-beta receptor, type I/II extracellular region family protein|<br /> Version = NM_001074096.1 GI:115484840 GeneID:4350145|<br /> Length = 5257 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0235200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:7176158..7181414|<br /> CDS = 7176608..7177532,7177841..7177965,7179701..7180180,7180759..7180976,7181105..7181243&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;gctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;ANMDVESRQGPKGGDTMAASEVSVDWRGRPCGSRKHGGMKAAVF VLGIQAFEMMAIAAVGNNLITYVFNEMHYPLSKSANIVTNFIGTVFLLSLLGGFLSDS YLGSFWTMLIFGFVELSGFILLAVQAHLPQLRPPACDMMAAAAAEGGCEEASGVKAGI FFAALYLVALGSGCLKPNIIAHGADQFRRGGGGGGDGDGDDGGDGKRLSSYFNAAYFS FCVGELVALTVLVWVQTRSGMDVGFGVSAAAMAVGLVSLVAGVFFYRNKPPQGSIFMP IAKVFVAAVTKRKQVCPSSSSTTAASHAVIPATGAAPVHRINKFRFLDKACVKVQDGH GGGGDGGKESVWPPCTPAEVEQVKVLLCVVPIFACTIVFNTILAQLQTFSVQQGSAMD TRIGGGGGGAAFHIPPASLQAIPYLVLIALVPAYEACFVPAMRRATGVDTGITPLQRI GVGLFAVTFSMVAAALVEAHRRRHAGDGLLSIFWIAPQFLVFGVSEMFTAVGLIEFFY KQSLSGMQAFLTSMTYCSYSFGFYLSSLLVSLVNKVTSGDGAGAGGGGWLSDNDLNKD RLDLFYWLLAGLSLLNFFNYLFWSRWYSKSVETTVQVAGVGGEGGGGEQQEEKGVDEV NMGH&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;3883..4807#3450..3574#1235..1714#439..656#172..310#gcctgaccacacaagcacaaagcaagcactatcaccaaaaggatcagagaagaaactgatatcactatctgatcagtgtagcattgcagcctctcttcttggtaggagtgagctagcacagtgaagagctaattaagccatttgtgtgagcttgcgttgagtgagctctgagctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctaggttcgtttaattagttagttactctcttaatccattatttcttgggtagaaatatgttgcttgatctgatttgttcttgtttgtgtttgtgttttgttggatatcgtttgcattgatgatgattgcagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgtaagaaattgattcatccatccttcatcgtcgatctcttctctttgatatcgcactacgataaaattgacattctttacacctagatgcaactttttgggtccttttttagactgcatttcttggctccttgcaagcaagaacagctgaacaggatcggatcttgtttgtttgtttgtttaatcatgtagggatgttgtttctaggtaggattatatataaaattaaccagataagacacgcgaaagattcagcgactgttcatcgtgccgcgaaaatgatgaggttttgaggaggaggcagcaagttcagcgtaaaggtgagagagagagtggaagaaaatcatggctgcctccatccaatgcggccatgctagctagtgcgccgcccaaaacgccactcgactccacaatatcatttcttggcttttgattttaattttcgagcaggttcattgcatcccatcccatgaccatatccatggcgataattaaccattactgattaatgcgccgccaactttagttagtttagctaataatttttgttgtttttttatttctaatttttcttggcaggggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggtacgtacgttatgccgtacaatacaatcgtcgtacgtgtacggttcgtcatgtacttttatacgctttcttcttttttttttttagagaaaaatatttcttacccggcctctacatccaatcagatatatgcagactttttaaattagaaacttaactctcaaacaatctaatctgaaacttgctactacgattaaactctagatctttaggatacgcttaggacattgcaaccacaattgcatcttcttttcttttctagttggcacattgaaatatcttttgatataattttgacagccatcttttttttaaatgaactctctgtttctaaaatataataacatagtattccattccatgtaacctagtactacgaatctatgtttaattgaactatgaatttagtagatttatagtactgtgttggtgtctattctagtactacgaatttatctcattctgttttagcatcttatattttaggagaggtaggattatttttaatcggacggagtatacaagttggaaacaaatactacatcattgtgttgcatttgcaaaagcagagccaaccaactctgtttttgtgccagctactatattcccctaaaaatattgttgcattcataaatattggaggtcaattttaagctaggtctatgttctctgttcctgcatatggtagttacttgaaaaatcctgaaaatatgataacgtgcattatggtgccatctgtcgctacacaaatatatagtaatatataagtataaaggttagagaaaacatggccgtgggccctagcaatgcgtttctgcactagctaaatagaatatatataatatgtgacaagactaattaatattaagaagcaccataggtagatcaaatttaaaaatgtaaggatgaaaaggcatgtgtatgtacaacaaattaaacaacagaaagtttgaccatacgtatcatcctgattccttgtttggaaagtactactccagatacgctgcttttatatactagtataaaagcaaaaccgcctattttcgttaaatataatgcataatctttatttgatccaaaaaagctacatgatcaaacaggagtttggtacagggactattttatcctcatagtcacctcgtgcactaccacataaaaagcacacgagatcatgggtttacaaccgtttctacattaaacaaaatgaaatgctacctagtttattgccaaatgaagtatttagtcaataaaggaaaagaaagcatatatatgatttactgaatgagataataatgtatatgtatgttgggataaaaaaaataaaactgatgattttaagttacacactggctggcctcctctgccttttctttcttctttctgggacaagttgacaagggctagtggctcagctttatttctctcttttgtgtgggtcaagaattaccactgtctgctgggaggtacctttgattattgatcacagaaatggaggcccattctgttatgcttgtgatacatcaatcatgtttgcatgtgtggggagagacatgtaacatgactgacaatgatctcaggacagcatatatatctttccagcttaattttgtttttagttcttgacttagatcggtataaaccccctaaaactcaattgcacgtgaaaataacaaactctcactcgatcgctgacattttgagctgatggtcggtacatgtgactcaatatgtataggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggtaggtaaaaaaatatttgtaagtggcactgtaatatactacttatttttaaaaaatatatggcaccgttaacttttcgcataatgtttgaccattcgtcttattcaaaaatttaatattaattcataaaaatataagttaagattatattttatttgataataaaacaagtcacaacaagataaaaataaaatttatataatttttttaataagattaatagataaatattatgccaaaagtcaaatggcgtgatatatttcgaaggagtataatagtttactcatttcatgtatgcatgcttacaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactgacatgtgggttcgaaccgcacgtctagtatattaggtggattattaatcatttaacctagtcttaaccttttttttatggtttttccttgtatggaaaaatattagtaagggtggatgtatatgagtccccacctaattaaggagagtgtgtgtgatgtataaattaattaatcaaaagggattaggtgattattacttagtaatgatccctgattaggtcaagtcaaaaggtagtagttctgagtttgactctgcaaggatgcaagagtagtggtatttgggtagggatcaagtgatcaagtacgtatctagtccttttctttaatgataaggagctagtatatatctatgtactttatatatgtagctatcatgtaaatgacaaaaggatttgccataaaagcaaagtactgataatagttggtgcaaaaaatactttcactgttgatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074096.1 RefSeq:Os11g0235200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0235200&diff=178376 2014-06-05T12:34:03Z

<p>Mayamei0424: /* Knowledge Expansion */</p> <hr /> <div>''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family,and regulates panicle development and seed size in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family. SP1 is preferentially expressed in young panicle，especially in the phloem of the branches of young panicle.SP1 regulates panicle development and seed size in rice.<br /> <br /> ===Mutation===<br /> [[File:SP1图1.png|right|thumb|250px|Figure1. Phenotype of the ''sp1-2'' mutant. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> ''sp1-2'' is one TOS17 insertion mutant. &lt;br&gt;<br /> '''(1) Phenotypic comparison between wild-type and ''sp1-2'' plants after heading.''' &lt;br&gt;<br /> The ''sp1-2'' mutant plant showed a slight reduction in plant height, owing to the shorted panicle (Figure 1a). &lt;br&gt;<br /> '''(2) Comparison of panicle sizes between the wild-type and ''sp1-2'' plants at the grain filling stage.''' &lt;br&gt;<br /> In comparison with the wild type, the mature panicle length of ''sp1-2'' was strikingly reduced (Figure 1b). &lt;br&gt;<br /> '''(3) Comparison of mature grains size between the wild type and ''sp1-2''.''' &lt;br&gt;<br /> The seed size of the ''sp1-2'' mutant was smaller than the wild type (Figure 1c). &lt;br&gt;<br /> '''(4) Comparison of mature grains weight between the wild type and ''sp1-2''.'''&lt;br&gt;<br /> In consisted with the smaller seeds, the weight of 1000 seeds was lower of the ''sp1-2'' mutant compared to the wild type (Figure 1d). &lt;br&gt;<br /> These results indicated that the ''SP1'' affects not only the panicle size, but also the grain size.<br /> <br /> ===Cloning of the ''SP1'' gene===<br /> [[File:SP1图2.png|right|thumb|250pxFigure 2. Map-based cloning and confirmation of the ''SP1'' gene. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> Map-based approach was used to clone the ''SP1'' gene. Primary gene mapping showed that the ''SP1''locus is located between the molecular markers S21074 and RM3701 on chromosome 11 (Figure 2a), and then fine mapping narrowed the locus to an 8-kb region between markers M7 and M8 (Figure 2b). Annotation of the 8-kb sequence identified an open reading frame (ORF), LOC_Os11g12740, which is composed of five exons and four introns (Figure 2c). DNA sequence comparison revealed a 31-bp deletion in the third exon in ''sp1-2''. The identity of ''SP1'' is further confirmed by genetic complementation. The plasmid pSPC containing the entire LOC_Os11g12740 ORF and pSPCT containing the partial coding region of the ORF were introduced into the ''sp1-2'' mutant (Figure 2d). All the 10 transgenic lines of pSPC showed a complementation of the sp1-2 phenotype, whereas all of the eight lines of pSPCT failed to rescue the ''sp1-2'' phenotype (Figure 2e,f). RT-PCR analysis showed that the transcription level of the ''SP1'' gene is dramatically decreased in young panicles of ''sp1-2'' (Figure 2g). These results presented above showed that the rice ''SP1'' gene and its mutation is responsible for the altered phenotype of sp1.<br /> <br /> ===Expression===<br /> [[File:SP1图3.png|right|thumb|250px|Figure 3. Expression pattern of ''SP1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> The spatial and temporal expression pattern of ''SP1'' is analyzed by GUS reporter system and RT-PCR.&lt;br&gt; <br /> As shown in figure 3a-m, weak GUS signals could be detected in panicle axes upon the formation of spikelets (Figure 3a), subsequently, the GUS signals become stronger in rachis, branches, stigmas, ovules, and the surfaces of palea and lemma during the elongation stage of the young panicle (Figure 3b-g). The GUS signals are barely detectable in mature panicle (Figure 3h). And the GUS signals are hardly detected in root, culm and leaf (Figure 3i-k). Interesting, the SP1-GUS fusion protein is specially located in the phloem of vascular bundles of panicle rachis (Figure 3l-m). RT-PCR analysis results indicated that ''SP1'' highly expressed in young panicle (Figure 3n).<br /> <br /> ===Evolution===<br /> [[File:SP1图4.png|left|thumb|250px|Figure 4. Phylogentic analysis of the ''SP1'' amino acid sequence with plant peptide transporter (PTR) proteins. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> Bioinformatic analysis revealed that the deduced amino acid sequence of ''SP1'' contains a conserved PTR2 domain, implying that ''SP1'' may encode a PTR family protein. Phylogenetic analysis showed that the ''SP1'' gene is classified into a distinct clade for nitrate transporters, and is closest to AtNRT1:2, an Arabidopsis nitrate transporter (Figure 4). &lt;br&gt;<br /> (Footnote: In Figure 4,the amino acid sequences of the entire proteins were aligned by CLUSTALX, and the phylogenetic tree was constructed using the neighbor-joining algorithm. Bootstrap values are shown at each node. The circle and the triangle indicate peptide and nitrate transporters, respectively. The square indicates a dicarboxylate transporter.)<br /> <br /> ===Knowledge Expansion===<br /> [[File:SP1图5.png|right|thumb|250px|Figure 5. ''SP1'' sequences and the predicted hydropathy profile of ''SP1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:SP1图6.png|left|thumb|250px|Figure 6. Subcellular localization of the SP1-GFP fusion protein. .(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> Most members of the PTR family contain 12 a-helical transmembrane domains, and are classified into four groups according to their structure &lt;ref name=&quot;ref2&quot;/&gt;. Hydropathy analysis shows that SP1 protein indeed contained 12 transmembrane domains (TMDs), harboring a long hydrophilic loop between TM6 and TM7 (Figure 5b,c). Three consensus sequences, GxxIADxWLGxFx TIxxxxxVxxxG, LGTGGIKPxV and FxxFYLxINxGSL, have been characterized for PTR proteins. However, these motifs were not strictly conserved in all PTR proteins &lt;ref name=&quot;ref3&quot;/&gt;&lt;ref name=&quot;ref4&quot;/&gt;. This is also the case in ''SP1'', in which there are several alternatives within the conserved motifs (GxxLSDxYLGxFxTMxxxxxVxxxG, LGSGCLKPxI and FxxAYFxFCxGEL). In consistent with the 12 TMDs, the ''SP1'' is located at the plasma membrane ,which is correlates with the ''SP1'' action ,as one nitrate transporter(figure 6). In contrast with the control, signal is observed ubiquitously in onion epidermal cells, in which the SP1-GFP fusion protein is exclusively localized in the plasma membrane (Figure 6a–d). And analysis of ACTIN:SP1-GFP transgenic rice also indicates that ''SP1'' localizes at the plasma membrane in rice (Figure 6e–f).&lt;br&gt;<br /> (Footnote: In Figure 5,(a) The nucleotide sequence and the deduced amino acid sequences of ''SP1''. Numbers at left refer to the positions of nucleic acids. The box highlights the 31-bp deletion in sp1-2. The asterisk indicates the stop codon introduced by the 31-bp deletion. The shaded letters refer to the conserved PTR2 domains, and the underlined letters indicate the 12 transmembrane domains. (b) Prediction of membrane-spanning regions and their orientation of ''SP1'' made by the TMPRED program. (c) A prediction model for the transmembrane topology of ''SP1''.)<br /> <br /> ==Labs working on this gene==<br /> *State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China,<br /> *State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences,Hangzhou 310006, China,<br /> *Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8652, Japan,<br /> *Research Institute for Bioresources, Okayama University, 2-20-1, Chuo, Kurashiki, Okayama 710-0046, Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os11g0235200|<br /> Description = TGF-beta receptor, type I/II extracellular region family protein|<br /> Version = NM_001074096.1 GI:115484840 GeneID:4350145|<br /> Length = 5257 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0235200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:7176158..7181414|<br /> CDS = 7176608..7177532,7177841..7177965,7179701..7180180,7180759..7180976,7181105..7181243&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;gctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;ANMDVESRQGPKGGDTMAASEVSVDWRGRPCGSRKHGGMKAAVF VLGIQAFEMMAIAAVGNNLITYVFNEMHYPLSKSANIVTNFIGTVFLLSLLGGFLSDS YLGSFWTMLIFGFVELSGFILLAVQAHLPQLRPPACDMMAAAAAEGGCEEASGVKAGI FFAALYLVALGSGCLKPNIIAHGADQFRRGGGGGGDGDGDDGGDGKRLSSYFNAAYFS FCVGELVALTVLVWVQTRSGMDVGFGVSAAAMAVGLVSLVAGVFFYRNKPPQGSIFMP IAKVFVAAVTKRKQVCPSSSSTTAASHAVIPATGAAPVHRINKFRFLDKACVKVQDGH GGGGDGGKESVWPPCTPAEVEQVKVLLCVVPIFACTIVFNTILAQLQTFSVQQGSAMD TRIGGGGGGAAFHIPPASLQAIPYLVLIALVPAYEACFVPAMRRATGVDTGITPLQRI GVGLFAVTFSMVAAALVEAHRRRHAGDGLLSIFWIAPQFLVFGVSEMFTAVGLIEFFY KQSLSGMQAFLTSMTYCSYSFGFYLSSLLVSLVNKVTSGDGAGAGGGGWLSDNDLNKD RLDLFYWLLAGLSLLNFFNYLFWSRWYSKSVETTVQVAGVGGEGGGGEQQEEKGVDEV NMGH&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;3883..4807#3450..3574#1235..1714#439..656#172..310#gcctgaccacacaagcacaaagcaagcactatcaccaaaaggatcagagaagaaactgatatcactatctgatcagtgtagcattgcagcctctcttcttggtaggagtgagctagcacagtgaagagctaattaagccatttgtgtgagcttgcgttgagtgagctctgagctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctaggttcgtttaattagttagttactctcttaatccattatttcttgggtagaaatatgttgcttgatctgatttgttcttgtttgtgtttgtgttttgttggatatcgtttgcattgatgatgattgcagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgtaagaaattgattcatccatccttcatcgtcgatctcttctctttgatatcgcactacgataaaattgacattctttacacctagatgcaactttttgggtccttttttagactgcatttcttggctccttgcaagcaagaacagctgaacaggatcggatcttgtttgtttgtttgtttaatcatgtagggatgttgtttctaggtaggattatatataaaattaaccagataagacacgcgaaagattcagcgactgttcatcgtgccgcgaaaatgatgaggttttgaggaggaggcagcaagttcagcgtaaaggtgagagagagagtggaagaaaatcatggctgcctccatccaatgcggccatgctagctagtgcgccgcccaaaacgccactcgactccacaatatcatttcttggcttttgattttaattttcgagcaggttcattgcatcccatcccatgaccatatccatggcgataattaaccattactgattaatgcgccgccaactttagttagtttagctaataatttttgttgtttttttatttctaatttttcttggcaggggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggtacgtacgttatgccgtacaatacaatcgtcgtacgtgtacggttcgtcatgtacttttatacgctttcttcttttttttttttagagaaaaatatttcttacccggcctctacatccaatcagatatatgcagactttttaaattagaaacttaactctcaaacaatctaatctgaaacttgctactacgattaaactctagatctttaggatacgcttaggacattgcaaccacaattgcatcttcttttcttttctagttggcacattgaaatatcttttgatataattttgacagccatcttttttttaaatgaactctctgtttctaaaatataataacatagtattccattccatgtaacctagtactacgaatctatgtttaattgaactatgaatttagtagatttatagtactgtgttggtgtctattctagtactacgaatttatctcattctgttttagcatcttatattttaggagaggtaggattatttttaatcggacggagtatacaagttggaaacaaatactacatcattgtgttgcatttgcaaaagcagagccaaccaactctgtttttgtgccagctactatattcccctaaaaatattgttgcattcataaatattggaggtcaattttaagctaggtctatgttctctgttcctgcatatggtagttacttgaaaaatcctgaaaatatgataacgtgcattatggtgccatctgtcgctacacaaatatatagtaatatataagtataaaggttagagaaaacatggccgtgggccctagcaatgcgtttctgcactagctaaatagaatatatataatatgtgacaagactaattaatattaagaagcaccataggtagatcaaatttaaaaatgtaaggatgaaaaggcatgtgtatgtacaacaaattaaacaacagaaagtttgaccatacgtatcatcctgattccttgtttggaaagtactactccagatacgctgcttttatatactagtataaaagcaaaaccgcctattttcgttaaatataatgcataatctttatttgatccaaaaaagctacatgatcaaacaggagtttggtacagggactattttatcctcatagtcacctcgtgcactaccacataaaaagcacacgagatcatgggtttacaaccgtttctacattaaacaaaatgaaatgctacctagtttattgccaaatgaagtatttagtcaataaaggaaaagaaagcatatatatgatttactgaatgagataataatgtatatgtatgttgggataaaaaaaataaaactgatgattttaagttacacactggctggcctcctctgccttttctttcttctttctgggacaagttgacaagggctagtggctcagctttatttctctcttttgtgtgggtcaagaattaccactgtctgctgggaggtacctttgattattgatcacagaaatggaggcccattctgttatgcttgtgatacatcaatcatgtttgcatgtgtggggagagacatgtaacatgactgacaatgatctcaggacagcatatatatctttccagcttaattttgtttttagttcttgacttagatcggtataaaccccctaaaactcaattgcacgtgaaaataacaaactctcactcgatcgctgacattttgagctgatggtcggtacatgtgactcaatatgtataggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggtaggtaaaaaaatatttgtaagtggcactgtaatatactacttatttttaaaaaatatatggcaccgttaacttttcgcataatgtttgaccattcgtcttattcaaaaatttaatattaattcataaaaatataagttaagattatattttatttgataataaaacaagtcacaacaagataaaaataaaatttatataatttttttaataagattaatagataaatattatgccaaaagtcaaatggcgtgatatatttcgaaggagtataatagtttactcatttcatgtatgcatgcttacaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactgacatgtgggttcgaaccgcacgtctagtatattaggtggattattaatcatttaacctagtcttaaccttttttttatggtttttccttgtatggaaaaatattagtaagggtggatgtatatgagtccccacctaattaaggagagtgtgtgtgatgtataaattaattaatcaaaagggattaggtgattattacttagtaatgatccctgattaggtcaagtcaaaaggtagtagttctgagtttgactctgcaaggatgcaagagtagtggtatttgggtagggatcaagtgatcaagtacgtatctagtccttttctttaatgataaggagctagtatatatctatgtactttatatatgtagctatcatgtaaatgacaaaaggatttgccataaaagcaaagtactgataatagttggtgcaaaaaatactttcactgttgatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074096.1 RefSeq:Os11g0235200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0235200&diff=178372 2014-06-05T12:25:59Z

<p>Mayamei0424: /* Annotated Information */</p> <hr /> <div>''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family,and regulates panicle development and seed size in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family. SP1 is preferentially expressed in young panicle，especially in the phloem of the branches of young panicle.SP1 regulates panicle development and seed size in rice.<br /> <br /> ===Mutation===<br /> [[File:SP1图1.png|right|thumb|250px|Figure1. Phenotype of the ''sp1-2'' mutant. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> ''sp1-2'' is one TOS17 insertion mutant. &lt;br&gt;<br /> '''(1) Phenotypic comparison between wild-type and ''sp1-2'' plants after heading.''' &lt;br&gt;<br /> The ''sp1-2'' mutant plant showed a slight reduction in plant height, owing to the shorted panicle (Figure 1a). &lt;br&gt;<br /> '''(2) Comparison of panicle sizes between the wild-type and ''sp1-2'' plants at the grain filling stage.''' &lt;br&gt;<br /> In comparison with the wild type, the mature panicle length of ''sp1-2'' was strikingly reduced (Figure 1b). &lt;br&gt;<br /> '''(3) Comparison of mature grains size between the wild type and ''sp1-2''.''' &lt;br&gt;<br /> The seed size of the ''sp1-2'' mutant was smaller than the wild type (Figure 1c). &lt;br&gt;<br /> '''(4) Comparison of mature grains weight between the wild type and ''sp1-2''.'''&lt;br&gt;<br /> In consisted with the smaller seeds, the weight of 1000 seeds was lower of the ''sp1-2'' mutant compared to the wild type (Figure 1d). &lt;br&gt;<br /> These results indicated that the ''SP1'' affects not only the panicle size, but also the grain size.<br /> <br /> ===Cloning of the ''SP1'' gene===<br /> [[File:SP1图2.png|right|thumb|250pxFigure 2. Map-based cloning and confirmation of the ''SP1'' gene. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> Map-based approach was used to clone the ''SP1'' gene. Primary gene mapping showed that the ''SP1''locus is located between the molecular markers S21074 and RM3701 on chromosome 11 (Figure 2a), and then fine mapping narrowed the locus to an 8-kb region between markers M7 and M8 (Figure 2b). Annotation of the 8-kb sequence identified an open reading frame (ORF), LOC_Os11g12740, which is composed of five exons and four introns (Figure 2c). DNA sequence comparison revealed a 31-bp deletion in the third exon in ''sp1-2''. The identity of ''SP1'' is further confirmed by genetic complementation. The plasmid pSPC containing the entire LOC_Os11g12740 ORF and pSPCT containing the partial coding region of the ORF were introduced into the ''sp1-2'' mutant (Figure 2d). All the 10 transgenic lines of pSPC showed a complementation of the sp1-2 phenotype, whereas all of the eight lines of pSPCT failed to rescue the ''sp1-2'' phenotype (Figure 2e,f). RT-PCR analysis showed that the transcription level of the ''SP1'' gene is dramatically decreased in young panicles of ''sp1-2'' (Figure 2g). These results presented above showed that the rice ''SP1'' gene and its mutation is responsible for the altered phenotype of sp1.<br /> <br /> ===Expression===<br /> [[File:SP1图3.png|right|thumb|250px|Figure 3. Expression pattern of ''SP1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> The spatial and temporal expression pattern of ''SP1'' is analyzed by GUS reporter system and RT-PCR.&lt;br&gt; <br /> As shown in figure 3a-m, weak GUS signals could be detected in panicle axes upon the formation of spikelets (Figure 3a), subsequently, the GUS signals become stronger in rachis, branches, stigmas, ovules, and the surfaces of palea and lemma during the elongation stage of the young panicle (Figure 3b-g). The GUS signals are barely detectable in mature panicle (Figure 3h). And the GUS signals are hardly detected in root, culm and leaf (Figure 3i-k). Interesting, the SP1-GUS fusion protein is specially located in the phloem of vascular bundles of panicle rachis (Figure 3l-m). RT-PCR analysis results indicated that ''SP1'' highly expressed in young panicle (Figure 3n).<br /> <br /> ===Evolution===<br /> [[File:SP1图4.png|left|thumb|250px|Figure 4. Phylogentic analysis of the ''SP1'' amino acid sequence with plant peptide transporter (PTR) proteins. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> Bioinformatic analysis revealed that the deduced amino acid sequence of ''SP1'' contains a conserved PTR2 domain, implying that ''SP1'' may encode a PTR family protein. Phylogenetic analysis showed that the ''SP1'' gene is classified into a distinct clade for nitrate transporters, and is closest to AtNRT1:2, an Arabidopsis nitrate transporter (Figure 4). &lt;br&gt;<br /> (Footnote: In Figure 4,the amino acid sequences of the entire proteins were aligned by CLUSTALX, and the phylogenetic tree was constructed using the neighbor-joining algorithm. Bootstrap values are shown at each node. The circle and the triangle indicate peptide and nitrate transporters, respectively. The square indicates a dicarboxylate transporter.)<br /> <br /> ===Knowledge Expansion===<br /> <br /> ==Labs working on this gene==<br /> *State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China,<br /> *State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences,Hangzhou 310006, China,<br /> *Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8652, Japan,<br /> *Research Institute for Bioresources, Okayama University, 2-20-1, Chuo, Kurashiki, Okayama 710-0046, Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os11g0235200|<br /> Description = TGF-beta receptor, type I/II extracellular region family protein|<br /> Version = NM_001074096.1 GI:115484840 GeneID:4350145|<br /> Length = 5257 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0235200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:7176158..7181414|<br /> CDS = 7176608..7177532,7177841..7177965,7179701..7180180,7180759..7180976,7181105..7181243&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;gctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;ANMDVESRQGPKGGDTMAASEVSVDWRGRPCGSRKHGGMKAAVF VLGIQAFEMMAIAAVGNNLITYVFNEMHYPLSKSANIVTNFIGTVFLLSLLGGFLSDS YLGSFWTMLIFGFVELSGFILLAVQAHLPQLRPPACDMMAAAAAEGGCEEASGVKAGI FFAALYLVALGSGCLKPNIIAHGADQFRRGGGGGGDGDGDDGGDGKRLSSYFNAAYFS FCVGELVALTVLVWVQTRSGMDVGFGVSAAAMAVGLVSLVAGVFFYRNKPPQGSIFMP IAKVFVAAVTKRKQVCPSSSSTTAASHAVIPATGAAPVHRINKFRFLDKACVKVQDGH GGGGDGGKESVWPPCTPAEVEQVKVLLCVVPIFACTIVFNTILAQLQTFSVQQGSAMD TRIGGGGGGAAFHIPPASLQAIPYLVLIALVPAYEACFVPAMRRATGVDTGITPLQRI GVGLFAVTFSMVAAALVEAHRRRHAGDGLLSIFWIAPQFLVFGVSEMFTAVGLIEFFY KQSLSGMQAFLTSMTYCSYSFGFYLSSLLVSLVNKVTSGDGAGAGGGGWLSDNDLNKD RLDLFYWLLAGLSLLNFFNYLFWSRWYSKSVETTVQVAGVGGEGGGGEQQEEKGVDEV NMGH&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;3883..4807#3450..3574#1235..1714#439..656#172..310#gcctgaccacacaagcacaaagcaagcactatcaccaaaaggatcagagaagaaactgatatcactatctgatcagtgtagcattgcagcctctcttcttggtaggagtgagctagcacagtgaagagctaattaagccatttgtgtgagcttgcgttgagtgagctctgagctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctaggttcgtttaattagttagttactctcttaatccattatttcttgggtagaaatatgttgcttgatctgatttgttcttgtttgtgtttgtgttttgttggatatcgtttgcattgatgatgattgcagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgtaagaaattgattcatccatccttcatcgtcgatctcttctctttgatatcgcactacgataaaattgacattctttacacctagatgcaactttttgggtccttttttagactgcatttcttggctccttgcaagcaagaacagctgaacaggatcggatcttgtttgtttgtttgtttaatcatgtagggatgttgtttctaggtaggattatatataaaattaaccagataagacacgcgaaagattcagcgactgttcatcgtgccgcgaaaatgatgaggttttgaggaggaggcagcaagttcagcgtaaaggtgagagagagagtggaagaaaatcatggctgcctccatccaatgcggccatgctagctagtgcgccgcccaaaacgccactcgactccacaatatcatttcttggcttttgattttaattttcgagcaggttcattgcatcccatcccatgaccatatccatggcgataattaaccattactgattaatgcgccgccaactttagttagtttagctaataatttttgttgtttttttatttctaatttttcttggcaggggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggtacgtacgttatgccgtacaatacaatcgtcgtacgtgtacggttcgtcatgtacttttatacgctttcttcttttttttttttagagaaaaatatttcttacccggcctctacatccaatcagatatatgcagactttttaaattagaaacttaactctcaaacaatctaatctgaaacttgctactacgattaaactctagatctttaggatacgcttaggacattgcaaccacaattgcatcttcttttcttttctagttggcacattgaaatatcttttgatataattttgacagccatcttttttttaaatgaactctctgtttctaaaatataataacatagtattccattccatgtaacctagtactacgaatctatgtttaattgaactatgaatttagtagatttatagtactgtgttggtgtctattctagtactacgaatttatctcattctgttttagcatcttatattttaggagaggtaggattatttttaatcggacggagtatacaagttggaaacaaatactacatcattgtgttgcatttgcaaaagcagagccaaccaactctgtttttgtgccagctactatattcccctaaaaatattgttgcattcataaatattggaggtcaattttaagctaggtctatgttctctgttcctgcatatggtagttacttgaaaaatcctgaaaatatgataacgtgcattatggtgccatctgtcgctacacaaatatatagtaatatataagtataaaggttagagaaaacatggccgtgggccctagcaatgcgtttctgcactagctaaatagaatatatataatatgtgacaagactaattaatattaagaagcaccataggtagatcaaatttaaaaatgtaaggatgaaaaggcatgtgtatgtacaacaaattaaacaacagaaagtttgaccatacgtatcatcctgattccttgtttggaaagtactactccagatacgctgcttttatatactagtataaaagcaaaaccgcctattttcgttaaatataatgcataatctttatttgatccaaaaaagctacatgatcaaacaggagtttggtacagggactattttatcctcatagtcacctcgtgcactaccacataaaaagcacacgagatcatgggtttacaaccgtttctacattaaacaaaatgaaatgctacctagtttattgccaaatgaagtatttagtcaataaaggaaaagaaagcatatatatgatttactgaatgagataataatgtatatgtatgttgggataaaaaaaataaaactgatgattttaagttacacactggctggcctcctctgccttttctttcttctttctgggacaagttgacaagggctagtggctcagctttatttctctcttttgtgtgggtcaagaattaccactgtctgctgggaggtacctttgattattgatcacagaaatggaggcccattctgttatgcttgtgatacatcaatcatgtttgcatgtgtggggagagacatgtaacatgactgacaatgatctcaggacagcatatatatctttccagcttaattttgtttttagttcttgacttagatcggtataaaccccctaaaactcaattgcacgtgaaaataacaaactctcactcgatcgctgacattttgagctgatggtcggtacatgtgactcaatatgtataggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggtaggtaaaaaaatatttgtaagtggcactgtaatatactacttatttttaaaaaatatatggcaccgttaacttttcgcataatgtttgaccattcgtcttattcaaaaatttaatattaattcataaaaatataagttaagattatattttatttgataataaaacaagtcacaacaagataaaaataaaatttatataatttttttaataagattaatagataaatattatgccaaaagtcaaatggcgtgatatatttcgaaggagtataatagtttactcatttcatgtatgcatgcttacaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactgacatgtgggttcgaaccgcacgtctagtatattaggtggattattaatcatttaacctagtcttaaccttttttttatggtttttccttgtatggaaaaatattagtaagggtggatgtatatgagtccccacctaattaaggagagtgtgtgtgatgtataaattaattaatcaaaagggattaggtgattattacttagtaatgatccctgattaggtcaagtcaaaaggtagtagttctgagtttgactctgcaaggatgcaagagtagtggtatttgggtagggatcaagtgatcaagtacgtatctagtccttttctttaatgataaggagctagtatatatctatgtactttatatatgtagctatcatgtaaatgacaaaaggatttgccataaaagcaaagtactgataatagttggtgcaaaaaatactttcactgttgatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074096.1 RefSeq:Os11g0235200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0235200&diff=178370 2014-06-05T12:24:49Z

<p>Mayamei0424: /* Evolution */</p> <hr /> <div>''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family,and regulates panicle development and seed size in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family. SP1 is preferentially expressed in young panicle，especially in the phloem of the branches of young panicle.SP1 regulates panicle development and seed size in rice.<br /> <br /> ===Mutation===<br /> [[File:SP1图1.png|right|thumb|250px|Figure1. Phenotype of the ''sp1-2'' mutant. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> ''sp1-2'' is one TOS17 insertion mutant. &lt;br&gt;<br /> '''(1) Phenotypic comparison between wild-type and ''sp1-2'' plants after heading.''' &lt;br&gt;<br /> The ''sp1-2'' mutant plant showed a slight reduction in plant height, owing to the shorted panicle (Figure 1a). &lt;br&gt;<br /> '''(2) Comparison of panicle sizes between the wild-type and ''sp1-2'' plants at the grain filling stage.''' &lt;br&gt;<br /> In comparison with the wild type, the mature panicle length of ''sp1-2'' was strikingly reduced (Figure 1b). &lt;br&gt;<br /> '''(3) Comparison of mature grains size between the wild type and ''sp1-2''.''' &lt;br&gt;<br /> The seed size of the ''sp1-2'' mutant was smaller than the wild type (Figure 1c). &lt;br&gt;<br /> '''(4) Comparison of mature grains weight between the wild type and ''sp1-2''.'''&lt;br&gt;<br /> In consisted with the smaller seeds, the weight of 1000 seeds was lower of the ''sp1-2'' mutant compared to the wild type (Figure 1d). &lt;br&gt;<br /> These results indicated that the ''SP1'' affects not only the panicle size, but also the grain size.<br /> <br /> ===Cloning of the ''SP1'' gene===<br /> [[File:SP1图2.png|right|thumb|250pxFigure 2. Map-based cloning and confirmation of the ''SP1'' gene. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> Map-based approach was used to clone the ''SP1'' gene. Primary gene mapping showed that the ''SP1''locus is located between the molecular markers S21074 and RM3701 on chromosome 11 (Figure 2a), and then fine mapping narrowed the locus to an 8-kb region between markers M7 and M8 (Figure 2b). Annotation of the 8-kb sequence identified an open reading frame (ORF), LOC_Os11g12740, which is composed of five exons and four introns (Figure 2c). DNA sequence comparison revealed a 31-bp deletion in the third exon in ''sp1-2''. The identity of ''SP1'' is further confirmed by genetic complementation. The plasmid pSPC containing the entire LOC_Os11g12740 ORF and pSPCT containing the partial coding region of the ORF were introduced into the ''sp1-2'' mutant (Figure 2d). All the 10 transgenic lines of pSPC showed a complementation of the sp1-2 phenotype, whereas all of the eight lines of pSPCT failed to rescue the ''sp1-2'' phenotype (Figure 2e,f). RT-PCR analysis showed that the transcription level of the ''SP1'' gene is dramatically decreased in young panicles of ''sp1-2'' (Figure 2g). These results presented above showed that the rice ''SP1'' gene and its mutation is responsible for the altered phenotype of sp1.<br /> <br /> ===Expression===<br /> [[File:SP1图3.png|right|thumb|250px|Figure 3. Expression pattern of ''SP1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> The spatial and temporal expression pattern of ''SP1'' is analyzed by GUS reporter system and RT-PCR.&lt;br&gt; <br /> As shown in figure 3a-m, weak GUS signals could be detected in panicle axes upon the formation of spikelets (Figure 3a), subsequently, the GUS signals become stronger in rachis, branches, stigmas, ovules, and the surfaces of palea and lemma during the elongation stage of the young panicle (Figure 3b-g). The GUS signals are barely detectable in mature panicle (Figure 3h). And the GUS signals are hardly detected in root, culm and leaf (Figure 3i-k). Interesting, the SP1-GUS fusion protein is specially located in the phloem of vascular bundles of panicle rachis (Figure 3l-m). RT-PCR analysis results indicated that ''SP1'' highly expressed in young panicle (Figure 3n).<br /> <br /> ===Evolution===<br /> [[File:SP1图4.png|left|thumb|250px|Figure 4. Phylogentic analysis of the ''SP1'' amino acid sequence with plant peptide transporter (PTR) proteins. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> Bioinformatic analysis revealed that the deduced amino acid sequence of ''SP1'' contains a conserved PTR2 domain, implying that ''SP1'' may encode a PTR family protein. Phylogenetic analysis showed that the ''SP1'' gene is classified into a distinct clade for nitrate transporters, and is closest to AtNRT1:2, an Arabidopsis nitrate transporter (Figure 4). &lt;br&gt;<br /> (Footnote: In Figure 4,the amino acid sequences of the entire proteins were aligned by CLUSTALX, and the phylogenetic tree was constructed using the neighbor-joining algorithm. Bootstrap values are shown at each node. The circle and the triangle indicate peptide and nitrate transporters, respectively. The square indicates a dicarboxylate transporter.)<br /> <br /> ==Labs working on this gene==<br /> *State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China,<br /> *State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences,Hangzhou 310006, China,<br /> *Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8652, Japan,<br /> *Research Institute for Bioresources, Okayama University, 2-20-1, Chuo, Kurashiki, Okayama 710-0046, Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os11g0235200|<br /> Description = TGF-beta receptor, type I/II extracellular region family protein|<br /> Version = NM_001074096.1 GI:115484840 GeneID:4350145|<br /> Length = 5257 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0235200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:7176158..7181414|<br /> CDS = 7176608..7177532,7177841..7177965,7179701..7180180,7180759..7180976,7181105..7181243&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;gctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;ANMDVESRQGPKGGDTMAASEVSVDWRGRPCGSRKHGGMKAAVF VLGIQAFEMMAIAAVGNNLITYVFNEMHYPLSKSANIVTNFIGTVFLLSLLGGFLSDS YLGSFWTMLIFGFVELSGFILLAVQAHLPQLRPPACDMMAAAAAEGGCEEASGVKAGI FFAALYLVALGSGCLKPNIIAHGADQFRRGGGGGGDGDGDDGGDGKRLSSYFNAAYFS FCVGELVALTVLVWVQTRSGMDVGFGVSAAAMAVGLVSLVAGVFFYRNKPPQGSIFMP IAKVFVAAVTKRKQVCPSSSSTTAASHAVIPATGAAPVHRINKFRFLDKACVKVQDGH GGGGDGGKESVWPPCTPAEVEQVKVLLCVVPIFACTIVFNTILAQLQTFSVQQGSAMD TRIGGGGGGAAFHIPPASLQAIPYLVLIALVPAYEACFVPAMRRATGVDTGITPLQRI GVGLFAVTFSMVAAALVEAHRRRHAGDGLLSIFWIAPQFLVFGVSEMFTAVGLIEFFY KQSLSGMQAFLTSMTYCSYSFGFYLSSLLVSLVNKVTSGDGAGAGGGGWLSDNDLNKD RLDLFYWLLAGLSLLNFFNYLFWSRWYSKSVETTVQVAGVGGEGGGGEQQEEKGVDEV NMGH&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;3883..4807#3450..3574#1235..1714#439..656#172..310#gcctgaccacacaagcacaaagcaagcactatcaccaaaaggatcagagaagaaactgatatcactatctgatcagtgtagcattgcagcctctcttcttggtaggagtgagctagcacagtgaagagctaattaagccatttgtgtgagcttgcgttgagtgagctctgagctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctaggttcgtttaattagttagttactctcttaatccattatttcttgggtagaaatatgttgcttgatctgatttgttcttgtttgtgtttgtgttttgttggatatcgtttgcattgatgatgattgcagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgtaagaaattgattcatccatccttcatcgtcgatctcttctctttgatatcgcactacgataaaattgacattctttacacctagatgcaactttttgggtccttttttagactgcatttcttggctccttgcaagcaagaacagctgaacaggatcggatcttgtttgtttgtttgtttaatcatgtagggatgttgtttctaggtaggattatatataaaattaaccagataagacacgcgaaagattcagcgactgttcatcgtgccgcgaaaatgatgaggttttgaggaggaggcagcaagttcagcgtaaaggtgagagagagagtggaagaaaatcatggctgcctccatccaatgcggccatgctagctagtgcgccgcccaaaacgccactcgactccacaatatcatttcttggcttttgattttaattttcgagcaggttcattgcatcccatcccatgaccatatccatggcgataattaaccattactgattaatgcgccgccaactttagttagtttagctaataatttttgttgtttttttatttctaatttttcttggcaggggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggtacgtacgttatgccgtacaatacaatcgtcgtacgtgtacggttcgtcatgtacttttatacgctttcttcttttttttttttagagaaaaatatttcttacccggcctctacatccaatcagatatatgcagactttttaaattagaaacttaactctcaaacaatctaatctgaaacttgctactacgattaaactctagatctttaggatacgcttaggacattgcaaccacaattgcatcttcttttcttttctagttggcacattgaaatatcttttgatataattttgacagccatcttttttttaaatgaactctctgtttctaaaatataataacatagtattccattccatgtaacctagtactacgaatctatgtttaattgaactatgaatttagtagatttatagtactgtgttggtgtctattctagtactacgaatttatctcattctgttttagcatcttatattttaggagaggtaggattatttttaatcggacggagtatacaagttggaaacaaatactacatcattgtgttgcatttgcaaaagcagagccaaccaactctgtttttgtgccagctactatattcccctaaaaatattgttgcattcataaatattggaggtcaattttaagctaggtctatgttctctgttcctgcatatggtagttacttgaaaaatcctgaaaatatgataacgtgcattatggtgccatctgtcgctacacaaatatatagtaatatataagtataaaggttagagaaaacatggccgtgggccctagcaatgcgtttctgcactagctaaatagaatatatataatatgtgacaagactaattaatattaagaagcaccataggtagatcaaatttaaaaatgtaaggatgaaaaggcatgtgtatgtacaacaaattaaacaacagaaagtttgaccatacgtatcatcctgattccttgtttggaaagtactactccagatacgctgcttttatatactagtataaaagcaaaaccgcctattttcgttaaatataatgcataatctttatttgatccaaaaaagctacatgatcaaacaggagtttggtacagggactattttatcctcatagtcacctcgtgcactaccacataaaaagcacacgagatcatgggtttacaaccgtttctacattaaacaaaatgaaatgctacctagtttattgccaaatgaagtatttagtcaataaaggaaaagaaagcatatatatgatttactgaatgagataataatgtatatgtatgttgggataaaaaaaataaaactgatgattttaagttacacactggctggcctcctctgccttttctttcttctttctgggacaagttgacaagggctagtggctcagctttatttctctcttttgtgtgggtcaagaattaccactgtctgctgggaggtacctttgattattgatcacagaaatggaggcccattctgttatgcttgtgatacatcaatcatgtttgcatgtgtggggagagacatgtaacatgactgacaatgatctcaggacagcatatatatctttccagcttaattttgtttttagttcttgacttagatcggtataaaccccctaaaactcaattgcacgtgaaaataacaaactctcactcgatcgctgacattttgagctgatggtcggtacatgtgactcaatatgtataggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggtaggtaaaaaaatatttgtaagtggcactgtaatatactacttatttttaaaaaatatatggcaccgttaacttttcgcataatgtttgaccattcgtcttattcaaaaatttaatattaattcataaaaatataagttaagattatattttatttgataataaaacaagtcacaacaagataaaaataaaatttatataatttttttaataagattaatagataaatattatgccaaaagtcaaatggcgtgatatatttcgaaggagtataatagtttactcatttcatgtatgcatgcttacaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactgacatgtgggttcgaaccgcacgtctagtatattaggtggattattaatcatttaacctagtcttaaccttttttttatggtttttccttgtatggaaaaatattagtaagggtggatgtatatgagtccccacctaattaaggagagtgtgtgtgatgtataaattaattaatcaaaagggattaggtgattattacttagtaatgatccctgattaggtcaagtcaaaaggtagtagttctgagtttgactctgcaaggatgcaagagtagtggtatttgggtagggatcaagtgatcaagtacgtatctagtccttttctttaatgataaggagctagtatatatctatgtactttatatatgtagctatcatgtaaatgacaaaaggatttgccataaaagcaaagtactgataatagttggtgcaaaaaatactttcactgttgatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074096.1 RefSeq:Os11g0235200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0235200&diff=178367 2014-06-05T12:22:00Z

<p>Mayamei0424: /* Annotated Information */</p> <hr /> <div>''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family,and regulates panicle development and seed size in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family. SP1 is preferentially expressed in young panicle，especially in the phloem of the branches of young panicle.SP1 regulates panicle development and seed size in rice.<br /> <br /> ===Mutation===<br /> [[File:SP1图1.png|right|thumb|250px|Figure1. Phenotype of the ''sp1-2'' mutant. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> ''sp1-2'' is one TOS17 insertion mutant. &lt;br&gt;<br /> '''(1) Phenotypic comparison between wild-type and ''sp1-2'' plants after heading.''' &lt;br&gt;<br /> The ''sp1-2'' mutant plant showed a slight reduction in plant height, owing to the shorted panicle (Figure 1a). &lt;br&gt;<br /> '''(2) Comparison of panicle sizes between the wild-type and ''sp1-2'' plants at the grain filling stage.''' &lt;br&gt;<br /> In comparison with the wild type, the mature panicle length of ''sp1-2'' was strikingly reduced (Figure 1b). &lt;br&gt;<br /> '''(3) Comparison of mature grains size between the wild type and ''sp1-2''.''' &lt;br&gt;<br /> The seed size of the ''sp1-2'' mutant was smaller than the wild type (Figure 1c). &lt;br&gt;<br /> '''(4) Comparison of mature grains weight between the wild type and ''sp1-2''.'''&lt;br&gt;<br /> In consisted with the smaller seeds, the weight of 1000 seeds was lower of the ''sp1-2'' mutant compared to the wild type (Figure 1d). &lt;br&gt;<br /> These results indicated that the ''SP1'' affects not only the panicle size, but also the grain size.<br /> <br /> ===Cloning of the ''SP1'' gene===<br /> [[File:SP1图2.png|right|thumb|250pxFigure 2. Map-based cloning and confirmation of the ''SP1'' gene. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> Map-based approach was used to clone the ''SP1'' gene. Primary gene mapping showed that the ''SP1''locus is located between the molecular markers S21074 and RM3701 on chromosome 11 (Figure 2a), and then fine mapping narrowed the locus to an 8-kb region between markers M7 and M8 (Figure 2b). Annotation of the 8-kb sequence identified an open reading frame (ORF), LOC_Os11g12740, which is composed of five exons and four introns (Figure 2c). DNA sequence comparison revealed a 31-bp deletion in the third exon in ''sp1-2''. The identity of ''SP1'' is further confirmed by genetic complementation. The plasmid pSPC containing the entire LOC_Os11g12740 ORF and pSPCT containing the partial coding region of the ORF were introduced into the ''sp1-2'' mutant (Figure 2d). All the 10 transgenic lines of pSPC showed a complementation of the sp1-2 phenotype, whereas all of the eight lines of pSPCT failed to rescue the ''sp1-2'' phenotype (Figure 2e,f). RT-PCR analysis showed that the transcription level of the ''SP1'' gene is dramatically decreased in young panicles of ''sp1-2'' (Figure 2g). These results presented above showed that the rice ''SP1'' gene and its mutation is responsible for the altered phenotype of sp1.<br /> <br /> ===Expression===<br /> [[File:SP1图3.png|right|thumb|250px|Figure 3. Expression pattern of ''SP1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> The spatial and temporal expression pattern of ''SP1'' is analyzed by GUS reporter system and RT-PCR.&lt;br&gt; <br /> As shown in figure 3a-m, weak GUS signals could be detected in panicle axes upon the formation of spikelets (Figure 3a), subsequently, the GUS signals become stronger in rachis, branches, stigmas, ovules, and the surfaces of palea and lemma during the elongation stage of the young panicle (Figure 3b-g). The GUS signals are barely detectable in mature panicle (Figure 3h). And the GUS signals are hardly detected in root, culm and leaf (Figure 3i-k). Interesting, the SP1-GUS fusion protein is specially located in the phloem of vascular bundles of panicle rachis (Figure 3l-m). RT-PCR analysis results indicated that ''SP1'' highly expressed in young panicle (Figure 3n).<br /> <br /> ===Evolution===<br /> <br /> ==Labs working on this gene==<br /> *State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China,<br /> *State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences,Hangzhou 310006, China,<br /> *Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8652, Japan,<br /> *Research Institute for Bioresources, Okayama University, 2-20-1, Chuo, Kurashiki, Okayama 710-0046, Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os11g0235200|<br /> Description = TGF-beta receptor, type I/II extracellular region family protein|<br /> Version = NM_001074096.1 GI:115484840 GeneID:4350145|<br /> Length = 5257 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0235200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:7176158..7181414|<br /> CDS = 7176608..7177532,7177841..7177965,7179701..7180180,7180759..7180976,7181105..7181243&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;gctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;ANMDVESRQGPKGGDTMAASEVSVDWRGRPCGSRKHGGMKAAVF VLGIQAFEMMAIAAVGNNLITYVFNEMHYPLSKSANIVTNFIGTVFLLSLLGGFLSDS YLGSFWTMLIFGFVELSGFILLAVQAHLPQLRPPACDMMAAAAAEGGCEEASGVKAGI FFAALYLVALGSGCLKPNIIAHGADQFRRGGGGGGDGDGDDGGDGKRLSSYFNAAYFS FCVGELVALTVLVWVQTRSGMDVGFGVSAAAMAVGLVSLVAGVFFYRNKPPQGSIFMP IAKVFVAAVTKRKQVCPSSSSTTAASHAVIPATGAAPVHRINKFRFLDKACVKVQDGH GGGGDGGKESVWPPCTPAEVEQVKVLLCVVPIFACTIVFNTILAQLQTFSVQQGSAMD TRIGGGGGGAAFHIPPASLQAIPYLVLIALVPAYEACFVPAMRRATGVDTGITPLQRI GVGLFAVTFSMVAAALVEAHRRRHAGDGLLSIFWIAPQFLVFGVSEMFTAVGLIEFFY KQSLSGMQAFLTSMTYCSYSFGFYLSSLLVSLVNKVTSGDGAGAGGGGWLSDNDLNKD RLDLFYWLLAGLSLLNFFNYLFWSRWYSKSVETTVQVAGVGGEGGGGEQQEEKGVDEV NMGH&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;3883..4807#3450..3574#1235..1714#439..656#172..310#gcctgaccacacaagcacaaagcaagcactatcaccaaaaggatcagagaagaaactgatatcactatctgatcagtgtagcattgcagcctctcttcttggtaggagtgagctagcacagtgaagagctaattaagccatttgtgtgagcttgcgttgagtgagctctgagctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctaggttcgtttaattagttagttactctcttaatccattatttcttgggtagaaatatgttgcttgatctgatttgttcttgtttgtgtttgtgttttgttggatatcgtttgcattgatgatgattgcagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgtaagaaattgattcatccatccttcatcgtcgatctcttctctttgatatcgcactacgataaaattgacattctttacacctagatgcaactttttgggtccttttttagactgcatttcttggctccttgcaagcaagaacagctgaacaggatcggatcttgtttgtttgtttgtttaatcatgtagggatgttgtttctaggtaggattatatataaaattaaccagataagacacgcgaaagattcagcgactgttcatcgtgccgcgaaaatgatgaggttttgaggaggaggcagcaagttcagcgtaaaggtgagagagagagtggaagaaaatcatggctgcctccatccaatgcggccatgctagctagtgcgccgcccaaaacgccactcgactccacaatatcatttcttggcttttgattttaattttcgagcaggttcattgcatcccatcccatgaccatatccatggcgataattaaccattactgattaatgcgccgccaactttagttagtttagctaataatttttgttgtttttttatttctaatttttcttggcaggggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggtacgtacgttatgccgtacaatacaatcgtcgtacgtgtacggttcgtcatgtacttttatacgctttcttcttttttttttttagagaaaaatatttcttacccggcctctacatccaatcagatatatgcagactttttaaattagaaacttaactctcaaacaatctaatctgaaacttgctactacgattaaactctagatctttaggatacgcttaggacattgcaaccacaattgcatcttcttttcttttctagttggcacattgaaatatcttttgatataattttgacagccatcttttttttaaatgaactctctgtttctaaaatataataacatagtattccattccatgtaacctagtactacgaatctatgtttaattgaactatgaatttagtagatttatagtactgtgttggtgtctattctagtactacgaatttatctcattctgttttagcatcttatattttaggagaggtaggattatttttaatcggacggagtatacaagttggaaacaaatactacatcattgtgttgcatttgcaaaagcagagccaaccaactctgtttttgtgccagctactatattcccctaaaaatattgttgcattcataaatattggaggtcaattttaagctaggtctatgttctctgttcctgcatatggtagttacttgaaaaatcctgaaaatatgataacgtgcattatggtgccatctgtcgctacacaaatatatagtaatatataagtataaaggttagagaaaacatggccgtgggccctagcaatgcgtttctgcactagctaaatagaatatatataatatgtgacaagactaattaatattaagaagcaccataggtagatcaaatttaaaaatgtaaggatgaaaaggcatgtgtatgtacaacaaattaaacaacagaaagtttgaccatacgtatcatcctgattccttgtttggaaagtactactccagatacgctgcttttatatactagtataaaagcaaaaccgcctattttcgttaaatataatgcataatctttatttgatccaaaaaagctacatgatcaaacaggagtttggtacagggactattttatcctcatagtcacctcgtgcactaccacataaaaagcacacgagatcatgggtttacaaccgtttctacattaaacaaaatgaaatgctacctagtttattgccaaatgaagtatttagtcaataaaggaaaagaaagcatatatatgatttactgaatgagataataatgtatatgtatgttgggataaaaaaaataaaactgatgattttaagttacacactggctggcctcctctgccttttctttcttctttctgggacaagttgacaagggctagtggctcagctttatttctctcttttgtgtgggtcaagaattaccactgtctgctgggaggtacctttgattattgatcacagaaatggaggcccattctgttatgcttgtgatacatcaatcatgtttgcatgtgtggggagagacatgtaacatgactgacaatgatctcaggacagcatatatatctttccagcttaattttgtttttagttcttgacttagatcggtataaaccccctaaaactcaattgcacgtgaaaataacaaactctcactcgatcgctgacattttgagctgatggtcggtacatgtgactcaatatgtataggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggtaggtaaaaaaatatttgtaagtggcactgtaatatactacttatttttaaaaaatatatggcaccgttaacttttcgcataatgtttgaccattcgtcttattcaaaaatttaatattaattcataaaaatataagttaagattatattttatttgataataaaacaagtcacaacaagataaaaataaaatttatataatttttttaataagattaatagataaatattatgccaaaagtcaaatggcgtgatatatttcgaaggagtataatagtttactcatttcatgtatgcatgcttacaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactgacatgtgggttcgaaccgcacgtctagtatattaggtggattattaatcatttaacctagtcttaaccttttttttatggtttttccttgtatggaaaaatattagtaagggtggatgtatatgagtccccacctaattaaggagagtgtgtgtgatgtataaattaattaatcaaaagggattaggtgattattacttagtaatgatccctgattaggtcaagtcaaaaggtagtagttctgagtttgactctgcaaggatgcaagagtagtggtatttgggtagggatcaagtgatcaagtacgtatctagtccttttctttaatgataaggagctagtatatatctatgtactttatatatgtagctatcatgtaaatgacaaaaggatttgccataaaagcaaagtactgataatagttggtgcaaaaaatactttcactgttgatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074096.1 RefSeq:Os11g0235200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0235200&diff=178366 2014-06-05T12:20:53Z

<p>Mayamei0424: /* Evolution */</p> <hr /> <div>''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family,and regulates panicle development and seed size in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family. SP1 is preferentially expressed in young panicle，especially in the phloem of the branches of young panicle.SP1 regulates panicle development and seed size in rice.<br /> <br /> ===Mutation===<br /> [[File:SP1图1.png|right|thumb|250px|Figure1. Phenotype of the ''sp1-2'' mutant. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> ''sp1-2'' is one TOS17 insertion mutant. &lt;br&gt;<br /> '''(1) Phenotypic comparison between wild-type and ''sp1-2'' plants after heading.''' &lt;br&gt;<br /> The ''sp1-2'' mutant plant showed a slight reduction in plant height, owing to the shorted panicle (Figure 1a). &lt;br&gt;<br /> '''(2) Comparison of panicle sizes between the wild-type and ''sp1-2'' plants at the grain filling stage.''' &lt;br&gt;<br /> In comparison with the wild type, the mature panicle length of ''sp1-2'' was strikingly reduced (Figure 1b). &lt;br&gt;<br /> '''(3) Comparison of mature grains size between the wild type and ''sp1-2''.''' &lt;br&gt;<br /> The seed size of the ''sp1-2'' mutant was smaller than the wild type (Figure 1c). &lt;br&gt;<br /> '''(4) Comparison of mature grains weight between the wild type and ''sp1-2''.'''&lt;br&gt;<br /> In consisted with the smaller seeds, the weight of 1000 seeds was lower of the ''sp1-2'' mutant compared to the wild type (Figure 1d). &lt;br&gt;<br /> These results indicated that the ''SP1'' affects not only the panicle size, but also the grain size.<br /> <br /> ===Cloning of the ''SP1'' gene===<br /> [[File:SP1图2.png|right|thumb|250pxFigure 2. Map-based cloning and confirmation of the ''SP1'' gene. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> Map-based approach was used to clone the ''SP1'' gene. Primary gene mapping showed that the ''SP1''locus is located between the molecular markers S21074 and RM3701 on chromosome 11 (Figure 2a), and then fine mapping narrowed the locus to an 8-kb region between markers M7 and M8 (Figure 2b). Annotation of the 8-kb sequence identified an open reading frame (ORF), LOC_Os11g12740, which is composed of five exons and four introns (Figure 2c). DNA sequence comparison revealed a 31-bp deletion in the third exon in ''sp1-2''. The identity of ''SP1'' is further confirmed by genetic complementation. The plasmid pSPC containing the entire LOC_Os11g12740 ORF and pSPCT containing the partial coding region of the ORF were introduced into the ''sp1-2'' mutant (Figure 2d). All the 10 transgenic lines of pSPC showed a complementation of the sp1-2 phenotype, whereas all of the eight lines of pSPCT failed to rescue the ''sp1-2'' phenotype (Figure 2e,f). RT-PCR analysis showed that the transcription level of the ''SP1'' gene is dramatically decreased in young panicles of ''sp1-2'' (Figure 2g). These results presented above showed that the rice ''SP1'' gene and its mutation is responsible for the altered phenotype of sp1.<br /> <br /> ===Expression===<br /> [[File:SP1图3.png|right|thumb|250px|Figure 3. Expression pattern of ''SP1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> The spatial and temporal expression pattern of ''SP1'' is analyzed by GUS reporter system and RT-PCR.&lt;br&gt; <br /> As shown in figure 3a-m, weak GUS signals could be detected in panicle axes upon the formation of spikelets (Figure 3a), subsequently, the GUS signals become stronger in rachis, branches, stigmas, ovules, and the surfaces of palea and lemma during the elongation stage of the young panicle (Figure 3b-g). The GUS signals are barely detectable in mature panicle (Figure 3h). And the GUS signals are hardly detected in root, culm and leaf (Figure 3i-k). Interesting, the SP1-GUS fusion protein is specially located in the phloem of vascular bundles of panicle rachis (Figure 3l-m). RT-PCR analysis results indicated that ''SP1'' highly expressed in young panicle (Figure 3n).<br /> <br /> ==Labs working on this gene==<br /> *State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China,<br /> *State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences,Hangzhou 310006, China,<br /> *Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8652, Japan,<br /> *Research Institute for Bioresources, Okayama University, 2-20-1, Chuo, Kurashiki, Okayama 710-0046, Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os11g0235200|<br /> Description = TGF-beta receptor, type I/II extracellular region family protein|<br /> Version = NM_001074096.1 GI:115484840 GeneID:4350145|<br /> Length = 5257 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0235200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:7176158..7181414|<br /> CDS = 7176608..7177532,7177841..7177965,7179701..7180180,7180759..7180976,7181105..7181243&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;gctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;ANMDVESRQGPKGGDTMAASEVSVDWRGRPCGSRKHGGMKAAVF VLGIQAFEMMAIAAVGNNLITYVFNEMHYPLSKSANIVTNFIGTVFLLSLLGGFLSDS YLGSFWTMLIFGFVELSGFILLAVQAHLPQLRPPACDMMAAAAAEGGCEEASGVKAGI FFAALYLVALGSGCLKPNIIAHGADQFRRGGGGGGDGDGDDGGDGKRLSSYFNAAYFS FCVGELVALTVLVWVQTRSGMDVGFGVSAAAMAVGLVSLVAGVFFYRNKPPQGSIFMP IAKVFVAAVTKRKQVCPSSSSTTAASHAVIPATGAAPVHRINKFRFLDKACVKVQDGH GGGGDGGKESVWPPCTPAEVEQVKVLLCVVPIFACTIVFNTILAQLQTFSVQQGSAMD TRIGGGGGGAAFHIPPASLQAIPYLVLIALVPAYEACFVPAMRRATGVDTGITPLQRI GVGLFAVTFSMVAAALVEAHRRRHAGDGLLSIFWIAPQFLVFGVSEMFTAVGLIEFFY KQSLSGMQAFLTSMTYCSYSFGFYLSSLLVSLVNKVTSGDGAGAGGGGWLSDNDLNKD RLDLFYWLLAGLSLLNFFNYLFWSRWYSKSVETTVQVAGVGGEGGGGEQQEEKGVDEV NMGH&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;3883..4807#3450..3574#1235..1714#439..656#172..310#gcctgaccacacaagcacaaagcaagcactatcaccaaaaggatcagagaagaaactgatatcactatctgatcagtgtagcattgcagcctctcttcttggtaggagtgagctagcacagtgaagagctaattaagccatttgtgtgagcttgcgttgagtgagctctgagctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctaggttcgtttaattagttagttactctcttaatccattatttcttgggtagaaatatgttgcttgatctgatttgttcttgtttgtgtttgtgttttgttggatatcgtttgcattgatgatgattgcagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgtaagaaattgattcatccatccttcatcgtcgatctcttctctttgatatcgcactacgataaaattgacattctttacacctagatgcaactttttgggtccttttttagactgcatttcttggctccttgcaagcaagaacagctgaacaggatcggatcttgtttgtttgtttgtttaatcatgtagggatgttgtttctaggtaggattatatataaaattaaccagataagacacgcgaaagattcagcgactgttcatcgtgccgcgaaaatgatgaggttttgaggaggaggcagcaagttcagcgtaaaggtgagagagagagtggaagaaaatcatggctgcctccatccaatgcggccatgctagctagtgcgccgcccaaaacgccactcgactccacaatatcatttcttggcttttgattttaattttcgagcaggttcattgcatcccatcccatgaccatatccatggcgataattaaccattactgattaatgcgccgccaactttagttagtttagctaataatttttgttgtttttttatttctaatttttcttggcaggggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggtacgtacgttatgccgtacaatacaatcgtcgtacgtgtacggttcgtcatgtacttttatacgctttcttcttttttttttttagagaaaaatatttcttacccggcctctacatccaatcagatatatgcagactttttaaattagaaacttaactctcaaacaatctaatctgaaacttgctactacgattaaactctagatctttaggatacgcttaggacattgcaaccacaattgcatcttcttttcttttctagttggcacattgaaatatcttttgatataattttgacagccatcttttttttaaatgaactctctgtttctaaaatataataacatagtattccattccatgtaacctagtactacgaatctatgtttaattgaactatgaatttagtagatttatagtactgtgttggtgtctattctagtactacgaatttatctcattctgttttagcatcttatattttaggagaggtaggattatttttaatcggacggagtatacaagttggaaacaaatactacatcattgtgttgcatttgcaaaagcagagccaaccaactctgtttttgtgccagctactatattcccctaaaaatattgttgcattcataaatattggaggtcaattttaagctaggtctatgttctctgttcctgcatatggtagttacttgaaaaatcctgaaaatatgataacgtgcattatggtgccatctgtcgctacacaaatatatagtaatatataagtataaaggttagagaaaacatggccgtgggccctagcaatgcgtttctgcactagctaaatagaatatatataatatgtgacaagactaattaatattaagaagcaccataggtagatcaaatttaaaaatgtaaggatgaaaaggcatgtgtatgtacaacaaattaaacaacagaaagtttgaccatacgtatcatcctgattccttgtttggaaagtactactccagatacgctgcttttatatactagtataaaagcaaaaccgcctattttcgttaaatataatgcataatctttatttgatccaaaaaagctacatgatcaaacaggagtttggtacagggactattttatcctcatagtcacctcgtgcactaccacataaaaagcacacgagatcatgggtttacaaccgtttctacattaaacaaaatgaaatgctacctagtttattgccaaatgaagtatttagtcaataaaggaaaagaaagcatatatatgatttactgaatgagataataatgtatatgtatgttgggataaaaaaaataaaactgatgattttaagttacacactggctggcctcctctgccttttctttcttctttctgggacaagttgacaagggctagtggctcagctttatttctctcttttgtgtgggtcaagaattaccactgtctgctgggaggtacctttgattattgatcacagaaatggaggcccattctgttatgcttgtgatacatcaatcatgtttgcatgtgtggggagagacatgtaacatgactgacaatgatctcaggacagcatatatatctttccagcttaattttgtttttagttcttgacttagatcggtataaaccccctaaaactcaattgcacgtgaaaataacaaactctcactcgatcgctgacattttgagctgatggtcggtacatgtgactcaatatgtataggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggtaggtaaaaaaatatttgtaagtggcactgtaatatactacttatttttaaaaaatatatggcaccgttaacttttcgcataatgtttgaccattcgtcttattcaaaaatttaatattaattcataaaaatataagttaagattatattttatttgataataaaacaagtcacaacaagataaaaataaaatttatataatttttttaataagattaatagataaatattatgccaaaagtcaaatggcgtgatatatttcgaaggagtataatagtttactcatttcatgtatgcatgcttacaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactgacatgtgggttcgaaccgcacgtctagtatattaggtggattattaatcatttaacctagtcttaaccttttttttatggtttttccttgtatggaaaaatattagtaagggtggatgtatatgagtccccacctaattaaggagagtgtgtgtgatgtataaattaattaatcaaaagggattaggtgattattacttagtaatgatccctgattaggtcaagtcaaaaggtagtagttctgagtttgactctgcaaggatgcaagagtagtggtatttgggtagggatcaagtgatcaagtacgtatctagtccttttctttaatgataaggagctagtatatatctatgtactttatatatgtagctatcatgtaaatgacaaaaggatttgccataaaagcaaagtactgataatagttggtgcaaaaaatactttcactgttgatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074096.1 RefSeq:Os11g0235200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0235200&diff=178363 2014-06-05T12:20:27Z

<p>Mayamei0424: /* Expression */</p> <hr /> <div>''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family,and regulates panicle development and seed size in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family. SP1 is preferentially expressed in young panicle，especially in the phloem of the branches of young panicle.SP1 regulates panicle development and seed size in rice.<br /> <br /> ===Mutation===<br /> [[File:SP1图1.png|right|thumb|250px|Figure1. Phenotype of the ''sp1-2'' mutant. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> ''sp1-2'' is one TOS17 insertion mutant. &lt;br&gt;<br /> '''(1) Phenotypic comparison between wild-type and ''sp1-2'' plants after heading.''' &lt;br&gt;<br /> The ''sp1-2'' mutant plant showed a slight reduction in plant height, owing to the shorted panicle (Figure 1a). &lt;br&gt;<br /> '''(2) Comparison of panicle sizes between the wild-type and ''sp1-2'' plants at the grain filling stage.''' &lt;br&gt;<br /> In comparison with the wild type, the mature panicle length of ''sp1-2'' was strikingly reduced (Figure 1b). &lt;br&gt;<br /> '''(3) Comparison of mature grains size between the wild type and ''sp1-2''.''' &lt;br&gt;<br /> The seed size of the ''sp1-2'' mutant was smaller than the wild type (Figure 1c). &lt;br&gt;<br /> '''(4) Comparison of mature grains weight between the wild type and ''sp1-2''.'''&lt;br&gt;<br /> In consisted with the smaller seeds, the weight of 1000 seeds was lower of the ''sp1-2'' mutant compared to the wild type (Figure 1d). &lt;br&gt;<br /> These results indicated that the ''SP1'' affects not only the panicle size, but also the grain size.<br /> <br /> ===Cloning of the ''SP1'' gene===<br /> [[File:SP1图2.png|right|thumb|250pxFigure 2. Map-based cloning and confirmation of the ''SP1'' gene. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> Map-based approach was used to clone the ''SP1'' gene. Primary gene mapping showed that the ''SP1''locus is located between the molecular markers S21074 and RM3701 on chromosome 11 (Figure 2a), and then fine mapping narrowed the locus to an 8-kb region between markers M7 and M8 (Figure 2b). Annotation of the 8-kb sequence identified an open reading frame (ORF), LOC_Os11g12740, which is composed of five exons and four introns (Figure 2c). DNA sequence comparison revealed a 31-bp deletion in the third exon in ''sp1-2''. The identity of ''SP1'' is further confirmed by genetic complementation. The plasmid pSPC containing the entire LOC_Os11g12740 ORF and pSPCT containing the partial coding region of the ORF were introduced into the ''sp1-2'' mutant (Figure 2d). All the 10 transgenic lines of pSPC showed a complementation of the sp1-2 phenotype, whereas all of the eight lines of pSPCT failed to rescue the ''sp1-2'' phenotype (Figure 2e,f). RT-PCR analysis showed that the transcription level of the ''SP1'' gene is dramatically decreased in young panicles of ''sp1-2'' (Figure 2g). These results presented above showed that the rice ''SP1'' gene and its mutation is responsible for the altered phenotype of sp1.<br /> <br /> ===Evolution===<br /> [[File:SP1图3.png|right|thumb|250px|Figure 3. Expression pattern of ''SP1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> The spatial and temporal expression pattern of ''SP1'' is analyzed by GUS reporter system and RT-PCR.&lt;br&gt; <br /> As shown in figure 3a-m, weak GUS signals could be detected in panicle axes upon the formation of spikelets (Figure 3a), subsequently, the GUS signals become stronger in rachis, branches, stigmas, ovules, and the surfaces of palea and lemma during the elongation stage of the young panicle (Figure 3b-g). The GUS signals are barely detectable in mature panicle (Figure 3h). And the GUS signals are hardly detected in root, culm and leaf (Figure 3i-k). Interesting, the SP1-GUS fusion protein is specially located in the phloem of vascular bundles of panicle rachis (Figure 3l-m). RT-PCR analysis results indicated that ''SP1'' highly expressed in young panicle (Figure 3n).<br /> <br /> ==Labs working on this gene==<br /> *State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China,<br /> *State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences,Hangzhou 310006, China,<br /> *Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8652, Japan,<br /> *Research Institute for Bioresources, Okayama University, 2-20-1, Chuo, Kurashiki, Okayama 710-0046, Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os11g0235200|<br /> Description = TGF-beta receptor, type I/II extracellular region family protein|<br /> Version = NM_001074096.1 GI:115484840 GeneID:4350145|<br /> Length = 5257 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0235200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:7176158..7181414|<br /> CDS = 7176608..7177532,7177841..7177965,7179701..7180180,7180759..7180976,7181105..7181243&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;gctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;ANMDVESRQGPKGGDTMAASEVSVDWRGRPCGSRKHGGMKAAVF VLGIQAFEMMAIAAVGNNLITYVFNEMHYPLSKSANIVTNFIGTVFLLSLLGGFLSDS YLGSFWTMLIFGFVELSGFILLAVQAHLPQLRPPACDMMAAAAAEGGCEEASGVKAGI FFAALYLVALGSGCLKPNIIAHGADQFRRGGGGGGDGDGDDGGDGKRLSSYFNAAYFS FCVGELVALTVLVWVQTRSGMDVGFGVSAAAMAVGLVSLVAGVFFYRNKPPQGSIFMP IAKVFVAAVTKRKQVCPSSSSTTAASHAVIPATGAAPVHRINKFRFLDKACVKVQDGH GGGGDGGKESVWPPCTPAEVEQVKVLLCVVPIFACTIVFNTILAQLQTFSVQQGSAMD TRIGGGGGGAAFHIPPASLQAIPYLVLIALVPAYEACFVPAMRRATGVDTGITPLQRI GVGLFAVTFSMVAAALVEAHRRRHAGDGLLSIFWIAPQFLVFGVSEMFTAVGLIEFFY KQSLSGMQAFLTSMTYCSYSFGFYLSSLLVSLVNKVTSGDGAGAGGGGWLSDNDLNKD RLDLFYWLLAGLSLLNFFNYLFWSRWYSKSVETTVQVAGVGGEGGGGEQQEEKGVDEV NMGH&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;3883..4807#3450..3574#1235..1714#439..656#172..310#gcctgaccacacaagcacaaagcaagcactatcaccaaaaggatcagagaagaaactgatatcactatctgatcagtgtagcattgcagcctctcttcttggtaggagtgagctagcacagtgaagagctaattaagccatttgtgtgagcttgcgttgagtgagctctgagctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctaggttcgtttaattagttagttactctcttaatccattatttcttgggtagaaatatgttgcttgatctgatttgttcttgtttgtgtttgtgttttgttggatatcgtttgcattgatgatgattgcagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgtaagaaattgattcatccatccttcatcgtcgatctcttctctttgatatcgcactacgataaaattgacattctttacacctagatgcaactttttgggtccttttttagactgcatttcttggctccttgcaagcaagaacagctgaacaggatcggatcttgtttgtttgtttgtttaatcatgtagggatgttgtttctaggtaggattatatataaaattaaccagataagacacgcgaaagattcagcgactgttcatcgtgccgcgaaaatgatgaggttttgaggaggaggcagcaagttcagcgtaaaggtgagagagagagtggaagaaaatcatggctgcctccatccaatgcggccatgctagctagtgcgccgcccaaaacgccactcgactccacaatatcatttcttggcttttgattttaattttcgagcaggttcattgcatcccatcccatgaccatatccatggcgataattaaccattactgattaatgcgccgccaactttagttagtttagctaataatttttgttgtttttttatttctaatttttcttggcaggggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggtacgtacgttatgccgtacaatacaatcgtcgtacgtgtacggttcgtcatgtacttttatacgctttcttcttttttttttttagagaaaaatatttcttacccggcctctacatccaatcagatatatgcagactttttaaattagaaacttaactctcaaacaatctaatctgaaacttgctactacgattaaactctagatctttaggatacgcttaggacattgcaaccacaattgcatcttcttttcttttctagttggcacattgaaatatcttttgatataattttgacagccatcttttttttaaatgaactctctgtttctaaaatataataacatagtattccattccatgtaacctagtactacgaatctatgtttaattgaactatgaatttagtagatttatagtactgtgttggtgtctattctagtactacgaatttatctcattctgttttagcatcttatattttaggagaggtaggattatttttaatcggacggagtatacaagttggaaacaaatactacatcattgtgttgcatttgcaaaagcagagccaaccaactctgtttttgtgccagctactatattcccctaaaaatattgttgcattcataaatattggaggtcaattttaagctaggtctatgttctctgttcctgcatatggtagttacttgaaaaatcctgaaaatatgataacgtgcattatggtgccatctgtcgctacacaaatatatagtaatatataagtataaaggttagagaaaacatggccgtgggccctagcaatgcgtttctgcactagctaaatagaatatatataatatgtgacaagactaattaatattaagaagcaccataggtagatcaaatttaaaaatgtaaggatgaaaaggcatgtgtatgtacaacaaattaaacaacagaaagtttgaccatacgtatcatcctgattccttgtttggaaagtactactccagatacgctgcttttatatactagtataaaagcaaaaccgcctattttcgttaaatataatgcataatctttatttgatccaaaaaagctacatgatcaaacaggagtttggtacagggactattttatcctcatagtcacctcgtgcactaccacataaaaagcacacgagatcatgggtttacaaccgtttctacattaaacaaaatgaaatgctacctagtttattgccaaatgaagtatttagtcaataaaggaaaagaaagcatatatatgatttactgaatgagataataatgtatatgtatgttgggataaaaaaaataaaactgatgattttaagttacacactggctggcctcctctgccttttctttcttctttctgggacaagttgacaagggctagtggctcagctttatttctctcttttgtgtgggtcaagaattaccactgtctgctgggaggtacctttgattattgatcacagaaatggaggcccattctgttatgcttgtgatacatcaatcatgtttgcatgtgtggggagagacatgtaacatgactgacaatgatctcaggacagcatatatatctttccagcttaattttgtttttagttcttgacttagatcggtataaaccccctaaaactcaattgcacgtgaaaataacaaactctcactcgatcgctgacattttgagctgatggtcggtacatgtgactcaatatgtataggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggtaggtaaaaaaatatttgtaagtggcactgtaatatactacttatttttaaaaaatatatggcaccgttaacttttcgcataatgtttgaccattcgtcttattcaaaaatttaatattaattcataaaaatataagttaagattatattttatttgataataaaacaagtcacaacaagataaaaataaaatttatataatttttttaataagattaatagataaatattatgccaaaagtcaaatggcgtgatatatttcgaaggagtataatagtttactcatttcatgtatgcatgcttacaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactgacatgtgggttcgaaccgcacgtctagtatattaggtggattattaatcatttaacctagtcttaaccttttttttatggtttttccttgtatggaaaaatattagtaagggtggatgtatatgagtccccacctaattaaggagagtgtgtgtgatgtataaattaattaatcaaaagggattaggtgattattacttagtaatgatccctgattaggtcaagtcaaaaggtagtagttctgagtttgactctgcaaggatgcaagagtagtggtatttgggtagggatcaagtgatcaagtacgtatctagtccttttctttaatgataaggagctagtatatatctatgtactttatatatgtagctatcatgtaaatgacaaaaggatttgccataaaagcaaagtactgataatagttggtgcaaaaaatactttcactgttgatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074096.1 RefSeq:Os11g0235200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0235200&diff=178358 2014-06-05T12:18:44Z

<p>Mayamei0424: /* Evolution */</p> <hr /> <div>''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family,and regulates panicle development and seed size in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family. SP1 is preferentially expressed in young panicle，especially in the phloem of the branches of young panicle.SP1 regulates panicle development and seed size in rice.<br /> <br /> ===Expression===<br /> [[File:SP1图1.png|right|thumb|250px|Figure1. Phenotype of the ''sp1-2'' mutant. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> ''sp1-2'' is one TOS17 insertion mutant. &lt;br&gt;<br /> '''(1) Phenotypic comparison between wild-type and ''sp1-2'' plants after heading.''' &lt;br&gt;<br /> The ''sp1-2'' mutant plant showed a slight reduction in plant height, owing to the shorted panicle (Figure 1a). &lt;br&gt;<br /> '''(2) Comparison of panicle sizes between the wild-type and ''sp1-2'' plants at the grain filling stage.''' &lt;br&gt;<br /> In comparison with the wild type, the mature panicle length of ''sp1-2'' was strikingly reduced (Figure 1b). &lt;br&gt;<br /> '''(3) Comparison of mature grains size between the wild type and ''sp1-2''.''' &lt;br&gt;<br /> The seed size of the ''sp1-2'' mutant was smaller than the wild type (Figure 1c). &lt;br&gt;<br /> '''(4) Comparison of mature grains weight between the wild type and ''sp1-2''.'''&lt;br&gt;<br /> In consisted with the smaller seeds, the weight of 1000 seeds was lower of the ''sp1-2'' mutant compared to the wild type (Figure 1d). &lt;br&gt;<br /> These results indicated that the ''SP1'' affects not only the panicle size, but also the grain size.<br /> <br /> ===Cloning of the ''SP1'' gene===<br /> [[File:SP1图2.png|right|thumb|250pxFigure 2. Map-based cloning and confirmation of the ''SP1'' gene. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> Map-based approach was used to clone the ''SP1'' gene. Primary gene mapping showed that the ''SP1''locus is located between the molecular markers S21074 and RM3701 on chromosome 11 (Figure 2a), and then fine mapping narrowed the locus to an 8-kb region between markers M7 and M8 (Figure 2b). Annotation of the 8-kb sequence identified an open reading frame (ORF), LOC_Os11g12740, which is composed of five exons and four introns (Figure 2c). DNA sequence comparison revealed a 31-bp deletion in the third exon in ''sp1-2''. The identity of ''SP1'' is further confirmed by genetic complementation. The plasmid pSPC containing the entire LOC_Os11g12740 ORF and pSPCT containing the partial coding region of the ORF were introduced into the ''sp1-2'' mutant (Figure 2d). All the 10 transgenic lines of pSPC showed a complementation of the sp1-2 phenotype, whereas all of the eight lines of pSPCT failed to rescue the ''sp1-2'' phenotype (Figure 2e,f). RT-PCR analysis showed that the transcription level of the ''SP1'' gene is dramatically decreased in young panicles of ''sp1-2'' (Figure 2g). These results presented above showed that the rice ''SP1'' gene and its mutation is responsible for the altered phenotype of sp1.<br /> <br /> ===Evolution===<br /> [[File:SP1图3.png|right|thumb|250px|Figure 3. Expression pattern of ''SP1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> The spatial and temporal expression pattern of ''SP1'' is analyzed by GUS reporter system and RT-PCR.&lt;br&gt; <br /> As shown in figure 3a-m, weak GUS signals could be detected in panicle axes upon the formation of spikelets (Figure 3a), subsequently, the GUS signals become stronger in rachis, branches, stigmas, ovules, and the surfaces of palea and lemma during the elongation stage of the young panicle (Figure 3b-g). The GUS signals are barely detectable in mature panicle (Figure 3h). And the GUS signals are hardly detected in root, culm and leaf (Figure 3i-k). Interesting, the SP1-GUS fusion protein is specially located in the phloem of vascular bundles of panicle rachis (Figure 3l-m). RT-PCR analysis results indicated that ''SP1'' highly expressed in young panicle (Figure 3n).<br /> <br /> ==Labs working on this gene==<br /> *State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China,<br /> *State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences,Hangzhou 310006, China,<br /> *Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8652, Japan,<br /> *Research Institute for Bioresources, Okayama University, 2-20-1, Chuo, Kurashiki, Okayama 710-0046, Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os11g0235200|<br /> Description = TGF-beta receptor, type I/II extracellular region family protein|<br /> Version = NM_001074096.1 GI:115484840 GeneID:4350145|<br /> Length = 5257 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0235200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:7176158..7181414|<br /> CDS = 7176608..7177532,7177841..7177965,7179701..7180180,7180759..7180976,7181105..7181243&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;gctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;ANMDVESRQGPKGGDTMAASEVSVDWRGRPCGSRKHGGMKAAVF VLGIQAFEMMAIAAVGNNLITYVFNEMHYPLSKSANIVTNFIGTVFLLSLLGGFLSDS YLGSFWTMLIFGFVELSGFILLAVQAHLPQLRPPACDMMAAAAAEGGCEEASGVKAGI FFAALYLVALGSGCLKPNIIAHGADQFRRGGGGGGDGDGDDGGDGKRLSSYFNAAYFS FCVGELVALTVLVWVQTRSGMDVGFGVSAAAMAVGLVSLVAGVFFYRNKPPQGSIFMP IAKVFVAAVTKRKQVCPSSSSTTAASHAVIPATGAAPVHRINKFRFLDKACVKVQDGH GGGGDGGKESVWPPCTPAEVEQVKVLLCVVPIFACTIVFNTILAQLQTFSVQQGSAMD TRIGGGGGGAAFHIPPASLQAIPYLVLIALVPAYEACFVPAMRRATGVDTGITPLQRI GVGLFAVTFSMVAAALVEAHRRRHAGDGLLSIFWIAPQFLVFGVSEMFTAVGLIEFFY KQSLSGMQAFLTSMTYCSYSFGFYLSSLLVSLVNKVTSGDGAGAGGGGWLSDNDLNKD RLDLFYWLLAGLSLLNFFNYLFWSRWYSKSVETTVQVAGVGGEGGGGEQQEEKGVDEV NMGH&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;3883..4807#3450..3574#1235..1714#439..656#172..310#gcctgaccacacaagcacaaagcaagcactatcaccaaaaggatcagagaagaaactgatatcactatctgatcagtgtagcattgcagcctctcttcttggtaggagtgagctagcacagtgaagagctaattaagccatttgtgtgagcttgcgttgagtgagctctgagctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctaggttcgtttaattagttagttactctcttaatccattatttcttgggtagaaatatgttgcttgatctgatttgttcttgtttgtgtttgtgttttgttggatatcgtttgcattgatgatgattgcagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgtaagaaattgattcatccatccttcatcgtcgatctcttctctttgatatcgcactacgataaaattgacattctttacacctagatgcaactttttgggtccttttttagactgcatttcttggctccttgcaagcaagaacagctgaacaggatcggatcttgtttgtttgtttgtttaatcatgtagggatgttgtttctaggtaggattatatataaaattaaccagataagacacgcgaaagattcagcgactgttcatcgtgccgcgaaaatgatgaggttttgaggaggaggcagcaagttcagcgtaaaggtgagagagagagtggaagaaaatcatggctgcctccatccaatgcggccatgctagctagtgcgccgcccaaaacgccactcgactccacaatatcatttcttggcttttgattttaattttcgagcaggttcattgcatcccatcccatgaccatatccatggcgataattaaccattactgattaatgcgccgccaactttagttagtttagctaataatttttgttgtttttttatttctaatttttcttggcaggggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggtacgtacgttatgccgtacaatacaatcgtcgtacgtgtacggttcgtcatgtacttttatacgctttcttcttttttttttttagagaaaaatatttcttacccggcctctacatccaatcagatatatgcagactttttaaattagaaacttaactctcaaacaatctaatctgaaacttgctactacgattaaactctagatctttaggatacgcttaggacattgcaaccacaattgcatcttcttttcttttctagttggcacattgaaatatcttttgatataattttgacagccatcttttttttaaatgaactctctgtttctaaaatataataacatagtattccattccatgtaacctagtactacgaatctatgtttaattgaactatgaatttagtagatttatagtactgtgttggtgtctattctagtactacgaatttatctcattctgttttagcatcttatattttaggagaggtaggattatttttaatcggacggagtatacaagttggaaacaaatactacatcattgtgttgcatttgcaaaagcagagccaaccaactctgtttttgtgccagctactatattcccctaaaaatattgttgcattcataaatattggaggtcaattttaagctaggtctatgttctctgttcctgcatatggtagttacttgaaaaatcctgaaaatatgataacgtgcattatggtgccatctgtcgctacacaaatatatagtaatatataagtataaaggttagagaaaacatggccgtgggccctagcaatgcgtttctgcactagctaaatagaatatatataatatgtgacaagactaattaatattaagaagcaccataggtagatcaaatttaaaaatgtaaggatgaaaaggcatgtgtatgtacaacaaattaaacaacagaaagtttgaccatacgtatcatcctgattccttgtttggaaagtactactccagatacgctgcttttatatactagtataaaagcaaaaccgcctattttcgttaaatataatgcataatctttatttgatccaaaaaagctacatgatcaaacaggagtttggtacagggactattttatcctcatagtcacctcgtgcactaccacataaaaagcacacgagatcatgggtttacaaccgtttctacattaaacaaaatgaaatgctacctagtttattgccaaatgaagtatttagtcaataaaggaaaagaaagcatatatatgatttactgaatgagataataatgtatatgtatgttgggataaaaaaaataaaactgatgattttaagttacacactggctggcctcctctgccttttctttcttctttctgggacaagttgacaagggctagtggctcagctttatttctctcttttgtgtgggtcaagaattaccactgtctgctgggaggtacctttgattattgatcacagaaatggaggcccattctgttatgcttgtgatacatcaatcatgtttgcatgtgtggggagagacatgtaacatgactgacaatgatctcaggacagcatatatatctttccagcttaattttgtttttagttcttgacttagatcggtataaaccccctaaaactcaattgcacgtgaaaataacaaactctcactcgatcgctgacattttgagctgatggtcggtacatgtgactcaatatgtataggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggtaggtaaaaaaatatttgtaagtggcactgtaatatactacttatttttaaaaaatatatggcaccgttaacttttcgcataatgtttgaccattcgtcttattcaaaaatttaatattaattcataaaaatataagttaagattatattttatttgataataaaacaagtcacaacaagataaaaataaaatttatataatttttttaataagattaatagataaatattatgccaaaagtcaaatggcgtgatatatttcgaaggagtataatagtttactcatttcatgtatgcatgcttacaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactgacatgtgggttcgaaccgcacgtctagtatattaggtggattattaatcatttaacctagtcttaaccttttttttatggtttttccttgtatggaaaaatattagtaagggtggatgtatatgagtccccacctaattaaggagagtgtgtgtgatgtataaattaattaatcaaaagggattaggtgattattacttagtaatgatccctgattaggtcaagtcaaaaggtagtagttctgagtttgactctgcaaggatgcaagagtagtggtatttgggtagggatcaagtgatcaagtacgtatctagtccttttctttaatgataaggagctagtatatatctatgtactttatatatgtagctatcatgtaaatgacaaaaggatttgccataaaagcaaagtactgataatagttggtgcaaaaaatactttcactgttgatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074096.1 RefSeq:Os11g0235200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0235200&diff=178347 2014-06-05T12:15:00Z

<p>Mayamei0424: /* Cloning of the SP1 gene */</p> <hr /> <div>''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family,and regulates panicle development and seed size in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family. SP1 is preferentially expressed in young panicle，especially in the phloem of the branches of young panicle.SP1 regulates panicle development and seed size in rice.<br /> <br /> ===Expression===<br /> [[File:SP1图1.png|right|thumb|250px|Figure1. Phenotype of the ''sp1-2'' mutant. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> ''sp1-2'' is one TOS17 insertion mutant. &lt;br&gt;<br /> '''(1) Phenotypic comparison between wild-type and ''sp1-2'' plants after heading.''' &lt;br&gt;<br /> The ''sp1-2'' mutant plant showed a slight reduction in plant height, owing to the shorted panicle (Figure 1a). &lt;br&gt;<br /> '''(2) Comparison of panicle sizes between the wild-type and ''sp1-2'' plants at the grain filling stage.''' &lt;br&gt;<br /> In comparison with the wild type, the mature panicle length of ''sp1-2'' was strikingly reduced (Figure 1b). &lt;br&gt;<br /> '''(3) Comparison of mature grains size between the wild type and ''sp1-2''.''' &lt;br&gt;<br /> The seed size of the ''sp1-2'' mutant was smaller than the wild type (Figure 1c). &lt;br&gt;<br /> '''(4) Comparison of mature grains weight between the wild type and ''sp1-2''.'''&lt;br&gt;<br /> In consisted with the smaller seeds, the weight of 1000 seeds was lower of the ''sp1-2'' mutant compared to the wild type (Figure 1d). &lt;br&gt;<br /> These results indicated that the ''SP1'' affects not only the panicle size, but also the grain size.<br /> <br /> ===Cloning of the ''SP1'' gene===<br /> [[File:SP1图2.png|right|thumb|250pxFigure 2. Map-based cloning and confirmation of the ''SP1'' gene. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> Map-based approach was used to clone the ''SP1'' gene. Primary gene mapping showed that the ''SP1''locus is located between the molecular markers S21074 and RM3701 on chromosome 11 (Figure 2a), and then fine mapping narrowed the locus to an 8-kb region between markers M7 and M8 (Figure 2b). Annotation of the 8-kb sequence identified an open reading frame (ORF), LOC_Os11g12740, which is composed of five exons and four introns (Figure 2c). DNA sequence comparison revealed a 31-bp deletion in the third exon in ''sp1-2''. The identity of ''SP1'' is further confirmed by genetic complementation. The plasmid pSPC containing the entire LOC_Os11g12740 ORF and pSPCT containing the partial coding region of the ORF were introduced into the ''sp1-2'' mutant (Figure 2d). All the 10 transgenic lines of pSPC showed a complementation of the sp1-2 phenotype, whereas all of the eight lines of pSPCT failed to rescue the ''sp1-2'' phenotype (Figure 2e,f). RT-PCR analysis showed that the transcription level of the ''SP1'' gene is dramatically decreased in young panicles of ''sp1-2'' (Figure 2g). These results presented above showed that the rice ''SP1'' gene and its mutation is responsible for the altered phenotype of sp1.<br /> <br /> ===Evolution===<br /> Phylogenetic analysis showed that the SP1 gene is classified into a distinct clade for nitrate transporters, and is closest to AtNRT1:2, an Arabidopsis nitrate transporter<br /> <br /> ==Labs working on this gene==<br /> *State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China,<br /> *State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences,Hangzhou 310006, China,<br /> *Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8652, Japan,<br /> *Research Institute for Bioresources, Okayama University, 2-20-1, Chuo, Kurashiki, Okayama 710-0046, Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os11g0235200|<br /> Description = TGF-beta receptor, type I/II extracellular region family protein|<br /> Version = NM_001074096.1 GI:115484840 GeneID:4350145|<br /> Length = 5257 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0235200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:7176158..7181414|<br /> CDS = 7176608..7177532,7177841..7177965,7179701..7180180,7180759..7180976,7181105..7181243&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;gctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;ANMDVESRQGPKGGDTMAASEVSVDWRGRPCGSRKHGGMKAAVF VLGIQAFEMMAIAAVGNNLITYVFNEMHYPLSKSANIVTNFIGTVFLLSLLGGFLSDS YLGSFWTMLIFGFVELSGFILLAVQAHLPQLRPPACDMMAAAAAEGGCEEASGVKAGI FFAALYLVALGSGCLKPNIIAHGADQFRRGGGGGGDGDGDDGGDGKRLSSYFNAAYFS FCVGELVALTVLVWVQTRSGMDVGFGVSAAAMAVGLVSLVAGVFFYRNKPPQGSIFMP IAKVFVAAVTKRKQVCPSSSSTTAASHAVIPATGAAPVHRINKFRFLDKACVKVQDGH GGGGDGGKESVWPPCTPAEVEQVKVLLCVVPIFACTIVFNTILAQLQTFSVQQGSAMD TRIGGGGGGAAFHIPPASLQAIPYLVLIALVPAYEACFVPAMRRATGVDTGITPLQRI GVGLFAVTFSMVAAALVEAHRRRHAGDGLLSIFWIAPQFLVFGVSEMFTAVGLIEFFY KQSLSGMQAFLTSMTYCSYSFGFYLSSLLVSLVNKVTSGDGAGAGGGGWLSDNDLNKD RLDLFYWLLAGLSLLNFFNYLFWSRWYSKSVETTVQVAGVGGEGGGGEQQEEKGVDEV NMGH&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;3883..4807#3450..3574#1235..1714#439..656#172..310#gcctgaccacacaagcacaaagcaagcactatcaccaaaaggatcagagaagaaactgatatcactatctgatcagtgtagcattgcagcctctcttcttggtaggagtgagctagcacagtgaagagctaattaagccatttgtgtgagcttgcgttgagtgagctctgagctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctaggttcgtttaattagttagttactctcttaatccattatttcttgggtagaaatatgttgcttgatctgatttgttcttgtttgtgtttgtgttttgttggatatcgtttgcattgatgatgattgcagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgtaagaaattgattcatccatccttcatcgtcgatctcttctctttgatatcgcactacgataaaattgacattctttacacctagatgcaactttttgggtccttttttagactgcatttcttggctccttgcaagcaagaacagctgaacaggatcggatcttgtttgtttgtttgtttaatcatgtagggatgttgtttctaggtaggattatatataaaattaaccagataagacacgcgaaagattcagcgactgttcatcgtgccgcgaaaatgatgaggttttgaggaggaggcagcaagttcagcgtaaaggtgagagagagagtggaagaaaatcatggctgcctccatccaatgcggccatgctagctagtgcgccgcccaaaacgccactcgactccacaatatcatttcttggcttttgattttaattttcgagcaggttcattgcatcccatcccatgaccatatccatggcgataattaaccattactgattaatgcgccgccaactttagttagtttagctaataatttttgttgtttttttatttctaatttttcttggcaggggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggtacgtacgttatgccgtacaatacaatcgtcgtacgtgtacggttcgtcatgtacttttatacgctttcttcttttttttttttagagaaaaatatttcttacccggcctctacatccaatcagatatatgcagactttttaaattagaaacttaactctcaaacaatctaatctgaaacttgctactacgattaaactctagatctttaggatacgcttaggacattgcaaccacaattgcatcttcttttcttttctagttggcacattgaaatatcttttgatataattttgacagccatcttttttttaaatgaactctctgtttctaaaatataataacatagtattccattccatgtaacctagtactacgaatctatgtttaattgaactatgaatttagtagatttatagtactgtgttggtgtctattctagtactacgaatttatctcattctgttttagcatcttatattttaggagaggtaggattatttttaatcggacggagtatacaagttggaaacaaatactacatcattgtgttgcatttgcaaaagcagagccaaccaactctgtttttgtgccagctactatattcccctaaaaatattgttgcattcataaatattggaggtcaattttaagctaggtctatgttctctgttcctgcatatggtagttacttgaaaaatcctgaaaatatgataacgtgcattatggtgccatctgtcgctacacaaatatatagtaatatataagtataaaggttagagaaaacatggccgtgggccctagcaatgcgtttctgcactagctaaatagaatatatataatatgtgacaagactaattaatattaagaagcaccataggtagatcaaatttaaaaatgtaaggatgaaaaggcatgtgtatgtacaacaaattaaacaacagaaagtttgaccatacgtatcatcctgattccttgtttggaaagtactactccagatacgctgcttttatatactagtataaaagcaaaaccgcctattttcgttaaatataatgcataatctttatttgatccaaaaaagctacatgatcaaacaggagtttggtacagggactattttatcctcatagtcacctcgtgcactaccacataaaaagcacacgagatcatgggtttacaaccgtttctacattaaacaaaatgaaatgctacctagtttattgccaaatgaagtatttagtcaataaaggaaaagaaagcatatatatgatttactgaatgagataataatgtatatgtatgttgggataaaaaaaataaaactgatgattttaagttacacactggctggcctcctctgccttttctttcttctttctgggacaagttgacaagggctagtggctcagctttatttctctcttttgtgtgggtcaagaattaccactgtctgctgggaggtacctttgattattgatcacagaaatggaggcccattctgttatgcttgtgatacatcaatcatgtttgcatgtgtggggagagacatgtaacatgactgacaatgatctcaggacagcatatatatctttccagcttaattttgtttttagttcttgacttagatcggtataaaccccctaaaactcaattgcacgtgaaaataacaaactctcactcgatcgctgacattttgagctgatggtcggtacatgtgactcaatatgtataggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggtaggtaaaaaaatatttgtaagtggcactgtaatatactacttatttttaaaaaatatatggcaccgttaacttttcgcataatgtttgaccattcgtcttattcaaaaatttaatattaattcataaaaatataagttaagattatattttatttgataataaaacaagtcacaacaagataaaaataaaatttatataatttttttaataagattaatagataaatattatgccaaaagtcaaatggcgtgatatatttcgaaggagtataatagtttactcatttcatgtatgcatgcttacaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactgacatgtgggttcgaaccgcacgtctagtatattaggtggattattaatcatttaacctagtcttaaccttttttttatggtttttccttgtatggaaaaatattagtaagggtggatgtatatgagtccccacctaattaaggagagtgtgtgtgatgtataaattaattaatcaaaagggattaggtgattattacttagtaatgatccctgattaggtcaagtcaaaaggtagtagttctgagtttgactctgcaaggatgcaagagtagtggtatttgggtagggatcaagtgatcaagtacgtatctagtccttttctttaatgataaggagctagtatatatctatgtactttatatatgtagctatcatgtaaatgacaaaaggatttgccataaaagcaaagtactgataatagttggtgcaaaaaatactttcactgttgatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074096.1 RefSeq:Os11g0235200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0235200&diff=178331 2014-06-05T12:10:00Z

<p>Mayamei0424: /* Annotated Information */</p> <hr /> <div>''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family,and regulates panicle development and seed size in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family. SP1 is preferentially expressed in young panicle，especially in the phloem of the branches of young panicle.SP1 regulates panicle development and seed size in rice.<br /> <br /> ===Expression===<br /> [[File:SP1图1.png|right|thumb|250px|Figure1. Phenotype of the ''sp1-2'' mutant. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> ''sp1-2'' is one TOS17 insertion mutant. &lt;br&gt;<br /> '''(1) Phenotypic comparison between wild-type and ''sp1-2'' plants after heading.''' &lt;br&gt;<br /> The ''sp1-2'' mutant plant showed a slight reduction in plant height, owing to the shorted panicle (Figure 1a). &lt;br&gt;<br /> '''(2) Comparison of panicle sizes between the wild-type and ''sp1-2'' plants at the grain filling stage.''' &lt;br&gt;<br /> In comparison with the wild type, the mature panicle length of ''sp1-2'' was strikingly reduced (Figure 1b). &lt;br&gt;<br /> '''(3) Comparison of mature grains size between the wild type and ''sp1-2''.''' &lt;br&gt;<br /> The seed size of the ''sp1-2'' mutant was smaller than the wild type (Figure 1c). &lt;br&gt;<br /> '''(4) Comparison of mature grains weight between the wild type and ''sp1-2''.'''&lt;br&gt;<br /> In consisted with the smaller seeds, the weight of 1000 seeds was lower of the ''sp1-2'' mutant compared to the wild type (Figure 1d). &lt;br&gt;<br /> These results indicated that the ''SP1'' affects not only the panicle size, but also the grain size.<br /> <br /> ===Cloning of the ''SP1'' gene===<br /> <br /> ===Evolution===<br /> Phylogenetic analysis showed that the SP1 gene is classified into a distinct clade for nitrate transporters, and is closest to AtNRT1:2, an Arabidopsis nitrate transporter<br /> <br /> ==Labs working on this gene==<br /> *State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China,<br /> *State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences,Hangzhou 310006, China,<br /> *Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8652, Japan,<br /> *Research Institute for Bioresources, Okayama University, 2-20-1, Chuo, Kurashiki, Okayama 710-0046, Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os11g0235200|<br /> Description = TGF-beta receptor, type I/II extracellular region family protein|<br /> Version = NM_001074096.1 GI:115484840 GeneID:4350145|<br /> Length = 5257 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0235200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:7176158..7181414|<br /> CDS = 7176608..7177532,7177841..7177965,7179701..7180180,7180759..7180976,7181105..7181243&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;gctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;ANMDVESRQGPKGGDTMAASEVSVDWRGRPCGSRKHGGMKAAVF VLGIQAFEMMAIAAVGNNLITYVFNEMHYPLSKSANIVTNFIGTVFLLSLLGGFLSDS YLGSFWTMLIFGFVELSGFILLAVQAHLPQLRPPACDMMAAAAAEGGCEEASGVKAGI FFAALYLVALGSGCLKPNIIAHGADQFRRGGGGGGDGDGDDGGDGKRLSSYFNAAYFS FCVGELVALTVLVWVQTRSGMDVGFGVSAAAMAVGLVSLVAGVFFYRNKPPQGSIFMP IAKVFVAAVTKRKQVCPSSSSTTAASHAVIPATGAAPVHRINKFRFLDKACVKVQDGH GGGGDGGKESVWPPCTPAEVEQVKVLLCVVPIFACTIVFNTILAQLQTFSVQQGSAMD TRIGGGGGGAAFHIPPASLQAIPYLVLIALVPAYEACFVPAMRRATGVDTGITPLQRI GVGLFAVTFSMVAAALVEAHRRRHAGDGLLSIFWIAPQFLVFGVSEMFTAVGLIEFFY KQSLSGMQAFLTSMTYCSYSFGFYLSSLLVSLVNKVTSGDGAGAGGGGWLSDNDLNKD RLDLFYWLLAGLSLLNFFNYLFWSRWYSKSVETTVQVAGVGGEGGGGEQQEEKGVDEV NMGH&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;3883..4807#3450..3574#1235..1714#439..656#172..310#gcctgaccacacaagcacaaagcaagcactatcaccaaaaggatcagagaagaaactgatatcactatctgatcagtgtagcattgcagcctctcttcttggtaggagtgagctagcacagtgaagagctaattaagccatttgtgtgagcttgcgttgagtgagctctgagctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctaggttcgtttaattagttagttactctcttaatccattatttcttgggtagaaatatgttgcttgatctgatttgttcttgtttgtgtttgtgttttgttggatatcgtttgcattgatgatgattgcagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgtaagaaattgattcatccatccttcatcgtcgatctcttctctttgatatcgcactacgataaaattgacattctttacacctagatgcaactttttgggtccttttttagactgcatttcttggctccttgcaagcaagaacagctgaacaggatcggatcttgtttgtttgtttgtttaatcatgtagggatgttgtttctaggtaggattatatataaaattaaccagataagacacgcgaaagattcagcgactgttcatcgtgccgcgaaaatgatgaggttttgaggaggaggcagcaagttcagcgtaaaggtgagagagagagtggaagaaaatcatggctgcctccatccaatgcggccatgctagctagtgcgccgcccaaaacgccactcgactccacaatatcatttcttggcttttgattttaattttcgagcaggttcattgcatcccatcccatgaccatatccatggcgataattaaccattactgattaatgcgccgccaactttagttagtttagctaataatttttgttgtttttttatttctaatttttcttggcaggggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggtacgtacgttatgccgtacaatacaatcgtcgtacgtgtacggttcgtcatgtacttttatacgctttcttcttttttttttttagagaaaaatatttcttacccggcctctacatccaatcagatatatgcagactttttaaattagaaacttaactctcaaacaatctaatctgaaacttgctactacgattaaactctagatctttaggatacgcttaggacattgcaaccacaattgcatcttcttttcttttctagttggcacattgaaatatcttttgatataattttgacagccatcttttttttaaatgaactctctgtttctaaaatataataacatagtattccattccatgtaacctagtactacgaatctatgtttaattgaactatgaatttagtagatttatagtactgtgttggtgtctattctagtactacgaatttatctcattctgttttagcatcttatattttaggagaggtaggattatttttaatcggacggagtatacaagttggaaacaaatactacatcattgtgttgcatttgcaaaagcagagccaaccaactctgtttttgtgccagctactatattcccctaaaaatattgttgcattcataaatattggaggtcaattttaagctaggtctatgttctctgttcctgcatatggtagttacttgaaaaatcctgaaaatatgataacgtgcattatggtgccatctgtcgctacacaaatatatagtaatatataagtataaaggttagagaaaacatggccgtgggccctagcaatgcgtttctgcactagctaaatagaatatatataatatgtgacaagactaattaatattaagaagcaccataggtagatcaaatttaaaaatgtaaggatgaaaaggcatgtgtatgtacaacaaattaaacaacagaaagtttgaccatacgtatcatcctgattccttgtttggaaagtactactccagatacgctgcttttatatactagtataaaagcaaaaccgcctattttcgttaaatataatgcataatctttatttgatccaaaaaagctacatgatcaaacaggagtttggtacagggactattttatcctcatagtcacctcgtgcactaccacataaaaagcacacgagatcatgggtttacaaccgtttctacattaaacaaaatgaaatgctacctagtttattgccaaatgaagtatttagtcaataaaggaaaagaaagcatatatatgatttactgaatgagataataatgtatatgtatgttgggataaaaaaaataaaactgatgattttaagttacacactggctggcctcctctgccttttctttcttctttctgggacaagttgacaagggctagtggctcagctttatttctctcttttgtgtgggtcaagaattaccactgtctgctgggaggtacctttgattattgatcacagaaatggaggcccattctgttatgcttgtgatacatcaatcatgtttgcatgtgtggggagagacatgtaacatgactgacaatgatctcaggacagcatatatatctttccagcttaattttgtttttagttcttgacttagatcggtataaaccccctaaaactcaattgcacgtgaaaataacaaactctcactcgatcgctgacattttgagctgatggtcggtacatgtgactcaatatgtataggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggtaggtaaaaaaatatttgtaagtggcactgtaatatactacttatttttaaaaaatatatggcaccgttaacttttcgcataatgtttgaccattcgtcttattcaaaaatttaatattaattcataaaaatataagttaagattatattttatttgataataaaacaagtcacaacaagataaaaataaaatttatataatttttttaataagattaatagataaatattatgccaaaagtcaaatggcgtgatatatttcgaaggagtataatagtttactcatttcatgtatgcatgcttacaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactgacatgtgggttcgaaccgcacgtctagtatattaggtggattattaatcatttaacctagtcttaaccttttttttatggtttttccttgtatggaaaaatattagtaagggtggatgtatatgagtccccacctaattaaggagagtgtgtgtgatgtataaattaattaatcaaaagggattaggtgattattacttagtaatgatccctgattaggtcaagtcaaaaggtagtagttctgagtttgactctgcaaggatgcaagagtagtggtatttgggtagggatcaagtgatcaagtacgtatctagtccttttctttaatgataaggagctagtatatatctatgtactttatatatgtagctatcatgtaaatgacaaaaggatttgccataaaagcaaagtactgataatagttggtgcaaaaaatactttcactgttgatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074096.1 RefSeq:Os11g0235200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0235200&diff=178327 2014-06-05T12:08:44Z

<p>Mayamei0424: /* Expression */</p> <hr /> <div>''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family,and regulates panicle development and seed size in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family. SP1 is preferentially expressed in young panicle，especially in the phloem of the branches of young panicle.SP1 regulates panicle development and seed size in rice.<br /> <br /> ===Expression===<br /> [[File:SP1图1.png|right|thumb|250px|Figure1. Phenotype of the ''sp1-2'' mutant. (from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> ''sp1-2'' is one TOS17 insertion mutant. &lt;br&gt;<br /> '''(1) Phenotypic comparison between wild-type and ''sp1-2'' plants after heading.''' &lt;br&gt;<br /> The ''sp1-2'' mutant plant showed a slight reduction in plant height, owing to the shorted panicle (Figure 1a). &lt;br&gt;<br /> '''(2) Comparison of panicle sizes between the wild-type and ''sp1-2'' plants at the grain filling stage.''' &lt;br&gt;<br /> In comparison with the wild type, the mature panicle length of ''sp1-2'' was strikingly reduced (Figure 1b). &lt;br&gt;<br /> '''(3) Comparison of mature grains size between the wild type and ''sp1-2''.''' &lt;br&gt;<br /> The seed size of the ''sp1-2'' mutant was smaller than the wild type (Figure 1c). &lt;br&gt;<br /> '''(4) Comparison of mature grains weight between the wild type and ''sp1-2''.'''&lt;br&gt;<br /> In consisted with the smaller seeds, the weight of 1000 seeds was lower of the ''sp1-2'' mutant compared to the wild type (Figure 1d). &lt;br&gt;<br /> These results indicated that the ''SP1'' affects not only the panicle size, but also the grain size.<br /> <br /> ===Evolution===<br /> Phylogenetic analysis showed that the SP1 gene is classified into a distinct clade for nitrate transporters, and is closest to AtNRT1:2, an Arabidopsis nitrate transporter<br /> <br /> ==Labs working on this gene==<br /> *State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China,<br /> *State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences,Hangzhou 310006, China,<br /> *Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8652, Japan,<br /> *Research Institute for Bioresources, Okayama University, 2-20-1, Chuo, Kurashiki, Okayama 710-0046, Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os11g0235200|<br /> Description = TGF-beta receptor, type I/II extracellular region family protein|<br /> Version = NM_001074096.1 GI:115484840 GeneID:4350145|<br /> Length = 5257 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0235200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:7176158..7181414|<br /> CDS = 7176608..7177532,7177841..7177965,7179701..7180180,7180759..7180976,7181105..7181243&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;gctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;ANMDVESRQGPKGGDTMAASEVSVDWRGRPCGSRKHGGMKAAVF VLGIQAFEMMAIAAVGNNLITYVFNEMHYPLSKSANIVTNFIGTVFLLSLLGGFLSDS YLGSFWTMLIFGFVELSGFILLAVQAHLPQLRPPACDMMAAAAAEGGCEEASGVKAGI FFAALYLVALGSGCLKPNIIAHGADQFRRGGGGGGDGDGDDGGDGKRLSSYFNAAYFS FCVGELVALTVLVWVQTRSGMDVGFGVSAAAMAVGLVSLVAGVFFYRNKPPQGSIFMP IAKVFVAAVTKRKQVCPSSSSTTAASHAVIPATGAAPVHRINKFRFLDKACVKVQDGH GGGGDGGKESVWPPCTPAEVEQVKVLLCVVPIFACTIVFNTILAQLQTFSVQQGSAMD TRIGGGGGGAAFHIPPASLQAIPYLVLIALVPAYEACFVPAMRRATGVDTGITPLQRI GVGLFAVTFSMVAAALVEAHRRRHAGDGLLSIFWIAPQFLVFGVSEMFTAVGLIEFFY KQSLSGMQAFLTSMTYCSYSFGFYLSSLLVSLVNKVTSGDGAGAGGGGWLSDNDLNKD RLDLFYWLLAGLSLLNFFNYLFWSRWYSKSVETTVQVAGVGGEGGGGEQQEEKGVDEV NMGH&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;3883..4807#3450..3574#1235..1714#439..656#172..310#gcctgaccacacaagcacaaagcaagcactatcaccaaaaggatcagagaagaaactgatatcactatctgatcagtgtagcattgcagcctctcttcttggtaggagtgagctagcacagtgaagagctaattaagccatttgtgtgagcttgcgttgagtgagctctgagctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctaggttcgtttaattagttagttactctcttaatccattatttcttgggtagaaatatgttgcttgatctgatttgttcttgtttgtgtttgtgttttgttggatatcgtttgcattgatgatgattgcagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgtaagaaattgattcatccatccttcatcgtcgatctcttctctttgatatcgcactacgataaaattgacattctttacacctagatgcaactttttgggtccttttttagactgcatttcttggctccttgcaagcaagaacagctgaacaggatcggatcttgtttgtttgtttgtttaatcatgtagggatgttgtttctaggtaggattatatataaaattaaccagataagacacgcgaaagattcagcgactgttcatcgtgccgcgaaaatgatgaggttttgaggaggaggcagcaagttcagcgtaaaggtgagagagagagtggaagaaaatcatggctgcctccatccaatgcggccatgctagctagtgcgccgcccaaaacgccactcgactccacaatatcatttcttggcttttgattttaattttcgagcaggttcattgcatcccatcccatgaccatatccatggcgataattaaccattactgattaatgcgccgccaactttagttagtttagctaataatttttgttgtttttttatttctaatttttcttggcaggggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggtacgtacgttatgccgtacaatacaatcgtcgtacgtgtacggttcgtcatgtacttttatacgctttcttcttttttttttttagagaaaaatatttcttacccggcctctacatccaatcagatatatgcagactttttaaattagaaacttaactctcaaacaatctaatctgaaacttgctactacgattaaactctagatctttaggatacgcttaggacattgcaaccacaattgcatcttcttttcttttctagttggcacattgaaatatcttttgatataattttgacagccatcttttttttaaatgaactctctgtttctaaaatataataacatagtattccattccatgtaacctagtactacgaatctatgtttaattgaactatgaatttagtagatttatagtactgtgttggtgtctattctagtactacgaatttatctcattctgttttagcatcttatattttaggagaggtaggattatttttaatcggacggagtatacaagttggaaacaaatactacatcattgtgttgcatttgcaaaagcagagccaaccaactctgtttttgtgccagctactatattcccctaaaaatattgttgcattcataaatattggaggtcaattttaagctaggtctatgttctctgttcctgcatatggtagttacttgaaaaatcctgaaaatatgataacgtgcattatggtgccatctgtcgctacacaaatatatagtaatatataagtataaaggttagagaaaacatggccgtgggccctagcaatgcgtttctgcactagctaaatagaatatatataatatgtgacaagactaattaatattaagaagcaccataggtagatcaaatttaaaaatgtaaggatgaaaaggcatgtgtatgtacaacaaattaaacaacagaaagtttgaccatacgtatcatcctgattccttgtttggaaagtactactccagatacgctgcttttatatactagtataaaagcaaaaccgcctattttcgttaaatataatgcataatctttatttgatccaaaaaagctacatgatcaaacaggagtttggtacagggactattttatcctcatagtcacctcgtgcactaccacataaaaagcacacgagatcatgggtttacaaccgtttctacattaaacaaaatgaaatgctacctagtttattgccaaatgaagtatttagtcaataaaggaaaagaaagcatatatatgatttactgaatgagataataatgtatatgtatgttgggataaaaaaaataaaactgatgattttaagttacacactggctggcctcctctgccttttctttcttctttctgggacaagttgacaagggctagtggctcagctttatttctctcttttgtgtgggtcaagaattaccactgtctgctgggaggtacctttgattattgatcacagaaatggaggcccattctgttatgcttgtgatacatcaatcatgtttgcatgtgtggggagagacatgtaacatgactgacaatgatctcaggacagcatatatatctttccagcttaattttgtttttagttcttgacttagatcggtataaaccccctaaaactcaattgcacgtgaaaataacaaactctcactcgatcgctgacattttgagctgatggtcggtacatgtgactcaatatgtataggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggtaggtaaaaaaatatttgtaagtggcactgtaatatactacttatttttaaaaaatatatggcaccgttaacttttcgcataatgtttgaccattcgtcttattcaaaaatttaatattaattcataaaaatataagttaagattatattttatttgataataaaacaagtcacaacaagataaaaataaaatttatataatttttttaataagattaatagataaatattatgccaaaagtcaaatggcgtgatatatttcgaaggagtataatagtttactcatttcatgtatgcatgcttacaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactgacatgtgggttcgaaccgcacgtctagtatattaggtggattattaatcatttaacctagtcttaaccttttttttatggtttttccttgtatggaaaaatattagtaagggtggatgtatatgagtccccacctaattaaggagagtgtgtgtgatgtataaattaattaatcaaaagggattaggtgattattacttagtaatgatccctgattaggtcaagtcaaaaggtagtagttctgagtttgactctgcaaggatgcaagagtagtggtatttgggtagggatcaagtgatcaagtacgtatctagtccttttctttaatgataaggagctagtatatatctatgtactttatatatgtagctatcatgtaaatgacaaaaggatttgccataaaagcaaagtactgataatagttggtgcaaaaaatactttcactgttgatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074096.1 RefSeq:Os11g0235200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0235200&diff=178314 2014-06-05T12:00:10Z

<p>Mayamei0424: /* Function */</p> <hr /> <div>''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family,and regulates panicle development and seed size in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family. SP1 is preferentially expressed in young panicle，especially in the phloem of the branches of young panicle.SP1 regulates panicle development and seed size in rice.<br /> <br /> ===Expression===<br /> sp1-2 is one TOS17 insertion mutant. The sp1 mutant plant showed a slight reduction in plant height, owing to the shorted panicle . In comparison with the wild type, the mature panicle length of Sp1-2 was strikingly reduced. Moreover, the seed size of the sp1-2 mutant was smaller than the wild type. In consisted with the smaller seeds, the weight of 1000 seeds was lower of the sp1-2 mutant compared to the wild type (Fig 1d). These results indicated that the SP1 affects not only the panicle size, but also the grain size.<br /> <br /> ===Evolution===<br /> Phylogenetic analysis showed that the SP1 gene is classified into a distinct clade for nitrate transporters, and is closest to AtNRT1:2, an Arabidopsis nitrate transporter<br /> <br /> ==Labs working on this gene==<br /> *State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China,<br /> *State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences,Hangzhou 310006, China,<br /> *Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8652, Japan,<br /> *Research Institute for Bioresources, Okayama University, 2-20-1, Chuo, Kurashiki, Okayama 710-0046, Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os11g0235200|<br /> Description = TGF-beta receptor, type I/II extracellular region family protein|<br /> Version = NM_001074096.1 GI:115484840 GeneID:4350145|<br /> Length = 5257 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0235200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:7176158..7181414|<br /> CDS = 7176608..7177532,7177841..7177965,7179701..7180180,7180759..7180976,7181105..7181243&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;gctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;ANMDVESRQGPKGGDTMAASEVSVDWRGRPCGSRKHGGMKAAVF VLGIQAFEMMAIAAVGNNLITYVFNEMHYPLSKSANIVTNFIGTVFLLSLLGGFLSDS YLGSFWTMLIFGFVELSGFILLAVQAHLPQLRPPACDMMAAAAAEGGCEEASGVKAGI FFAALYLVALGSGCLKPNIIAHGADQFRRGGGGGGDGDGDDGGDGKRLSSYFNAAYFS FCVGELVALTVLVWVQTRSGMDVGFGVSAAAMAVGLVSLVAGVFFYRNKPPQGSIFMP IAKVFVAAVTKRKQVCPSSSSTTAASHAVIPATGAAPVHRINKFRFLDKACVKVQDGH GGGGDGGKESVWPPCTPAEVEQVKVLLCVVPIFACTIVFNTILAQLQTFSVQQGSAMD TRIGGGGGGAAFHIPPASLQAIPYLVLIALVPAYEACFVPAMRRATGVDTGITPLQRI GVGLFAVTFSMVAAALVEAHRRRHAGDGLLSIFWIAPQFLVFGVSEMFTAVGLIEFFY KQSLSGMQAFLTSMTYCSYSFGFYLSSLLVSLVNKVTSGDGAGAGGGGWLSDNDLNKD RLDLFYWLLAGLSLLNFFNYLFWSRWYSKSVETTVQVAGVGGEGGGGEQQEEKGVDEV NMGH&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;3883..4807#3450..3574#1235..1714#439..656#172..310#gcctgaccacacaagcacaaagcaagcactatcaccaaaaggatcagagaagaaactgatatcactatctgatcagtgtagcattgcagcctctcttcttggtaggagtgagctagcacagtgaagagctaattaagccatttgtgtgagcttgcgttgagtgagctctgagctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctaggttcgtttaattagttagttactctcttaatccattatttcttgggtagaaatatgttgcttgatctgatttgttcttgtttgtgtttgtgttttgttggatatcgtttgcattgatgatgattgcagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgtaagaaattgattcatccatccttcatcgtcgatctcttctctttgatatcgcactacgataaaattgacattctttacacctagatgcaactttttgggtccttttttagactgcatttcttggctccttgcaagcaagaacagctgaacaggatcggatcttgtttgtttgtttgtttaatcatgtagggatgttgtttctaggtaggattatatataaaattaaccagataagacacgcgaaagattcagcgactgttcatcgtgccgcgaaaatgatgaggttttgaggaggaggcagcaagttcagcgtaaaggtgagagagagagtggaagaaaatcatggctgcctccatccaatgcggccatgctagctagtgcgccgcccaaaacgccactcgactccacaatatcatttcttggcttttgattttaattttcgagcaggttcattgcatcccatcccatgaccatatccatggcgataattaaccattactgattaatgcgccgccaactttagttagtttagctaataatttttgttgtttttttatttctaatttttcttggcaggggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggtacgtacgttatgccgtacaatacaatcgtcgtacgtgtacggttcgtcatgtacttttatacgctttcttcttttttttttttagagaaaaatatttcttacccggcctctacatccaatcagatatatgcagactttttaaattagaaacttaactctcaaacaatctaatctgaaacttgctactacgattaaactctagatctttaggatacgcttaggacattgcaaccacaattgcatcttcttttcttttctagttggcacattgaaatatcttttgatataattttgacagccatcttttttttaaatgaactctctgtttctaaaatataataacatagtattccattccatgtaacctagtactacgaatctatgtttaattgaactatgaatttagtagatttatagtactgtgttggtgtctattctagtactacgaatttatctcattctgttttagcatcttatattttaggagaggtaggattatttttaatcggacggagtatacaagttggaaacaaatactacatcattgtgttgcatttgcaaaagcagagccaaccaactctgtttttgtgccagctactatattcccctaaaaatattgttgcattcataaatattggaggtcaattttaagctaggtctatgttctctgttcctgcatatggtagttacttgaaaaatcctgaaaatatgataacgtgcattatggtgccatctgtcgctacacaaatatatagtaatatataagtataaaggttagagaaaacatggccgtgggccctagcaatgcgtttctgcactagctaaatagaatatatataatatgtgacaagactaattaatattaagaagcaccataggtagatcaaatttaaaaatgtaaggatgaaaaggcatgtgtatgtacaacaaattaaacaacagaaagtttgaccatacgtatcatcctgattccttgtttggaaagtactactccagatacgctgcttttatatactagtataaaagcaaaaccgcctattttcgttaaatataatgcataatctttatttgatccaaaaaagctacatgatcaaacaggagtttggtacagggactattttatcctcatagtcacctcgtgcactaccacataaaaagcacacgagatcatgggtttacaaccgtttctacattaaacaaaatgaaatgctacctagtttattgccaaatgaagtatttagtcaataaaggaaaagaaagcatatatatgatttactgaatgagataataatgtatatgtatgttgggataaaaaaaataaaactgatgattttaagttacacactggctggcctcctctgccttttctttcttctttctgggacaagttgacaagggctagtggctcagctttatttctctcttttgtgtgggtcaagaattaccactgtctgctgggaggtacctttgattattgatcacagaaatggaggcccattctgttatgcttgtgatacatcaatcatgtttgcatgtgtggggagagacatgtaacatgactgacaatgatctcaggacagcatatatatctttccagcttaattttgtttttagttcttgacttagatcggtataaaccccctaaaactcaattgcacgtgaaaataacaaactctcactcgatcgctgacattttgagctgatggtcggtacatgtgactcaatatgtataggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggtaggtaaaaaaatatttgtaagtggcactgtaatatactacttatttttaaaaaatatatggcaccgttaacttttcgcataatgtttgaccattcgtcttattcaaaaatttaatattaattcataaaaatataagttaagattatattttatttgataataaaacaagtcacaacaagataaaaataaaatttatataatttttttaataagattaatagataaatattatgccaaaagtcaaatggcgtgatatatttcgaaggagtataatagtttactcatttcatgtatgcatgcttacaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactgacatgtgggttcgaaccgcacgtctagtatattaggtggattattaatcatttaacctagtcttaaccttttttttatggtttttccttgtatggaaaaatattagtaagggtggatgtatatgagtccccacctaattaaggagagtgtgtgtgatgtataaattaattaatcaaaagggattaggtgattattacttagtaatgatccctgattaggtcaagtcaaaaggtagtagttctgagtttgactctgcaaggatgcaagagtagtggtatttgggtagggatcaagtgatcaagtacgtatctagtccttttctttaatgataaggagctagtatatatctatgtactttatatatgtagctatcatgtaaatgacaaaaggatttgccataaaagcaaagtactgataatagttggtgcaaaaaatactttcactgttgatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074096.1 RefSeq:Os11g0235200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:SP1%E5%9B%BE6.png&diff=178309 2014-06-05T11:58:22Z

<p>Mayamei0424: </p> <hr /> <div></div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:SP1%E5%9B%BE5.png&diff=178308 2014-06-05T11:58:03Z

<p>Mayamei0424: </p> <hr /> <div></div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:SP1%E5%9B%BE4.png&diff=178306 2014-06-05T11:57:42Z

<p>Mayamei0424: </p> <hr /> <div></div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:SP1%E5%9B%BE3.png&diff=178305 2014-06-05T11:57:25Z

<p>Mayamei0424: </p> <hr /> <div></div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:SP1%E5%9B%BE2.png&diff=178303 2014-06-05T11:57:05Z

<p>Mayamei0424: </p> <hr /> <div></div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:SP1%E5%9B%BE1.png&diff=178302 2014-06-05T11:56:44Z

<p>Mayamei0424: </p> <hr /> <div></div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0235200&diff=178297 2014-06-05T11:54:57Z

<p>Mayamei0424: </p> <hr /> <div>''SP1'' encodes a putative transporter that belongs to the peptide transporter (PTR) family,and regulates panicle development and seed size in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> SP1 encodes a putative transporter that belongs to the peptide transporter (PTR) family. SP1 is preferentially expressed in young panicle，especially in the phloem of the branches of young panicle. The sp1-2 mutant showed strikingly reduced panicle and seed size.<br /> <br /> ===Expression===<br /> sp1-2 is one TOS17 insertion mutant. The sp1 mutant plant showed a slight reduction in plant height, owing to the shorted panicle . In comparison with the wild type, the mature panicle length of Sp1-2 was strikingly reduced. Moreover, the seed size of the sp1-2 mutant was smaller than the wild type. In consisted with the smaller seeds, the weight of 1000 seeds was lower of the sp1-2 mutant compared to the wild type (Fig 1d). These results indicated that the SP1 affects not only the panicle size, but also the grain size.<br /> <br /> ===Evolution===<br /> Phylogenetic analysis showed that the SP1 gene is classified into a distinct clade for nitrate transporters, and is closest to AtNRT1:2, an Arabidopsis nitrate transporter<br /> <br /> ==Labs working on this gene==<br /> *State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China,<br /> *State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences,Hangzhou 310006, China,<br /> *Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8652, Japan,<br /> *Research Institute for Bioresources, Okayama University, 2-20-1, Chuo, Kurashiki, Okayama 710-0046, Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os11g0235200|<br /> Description = TGF-beta receptor, type I/II extracellular region family protein|<br /> Version = NM_001074096.1 GI:115484840 GeneID:4350145|<br /> Length = 5257 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0235200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:7176158..7181414|<br /> CDS = 7176608..7177532,7177841..7177965,7179701..7180180,7180759..7180976,7181105..7181243&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;gctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;ANMDVESRQGPKGGDTMAASEVSVDWRGRPCGSRKHGGMKAAVF VLGIQAFEMMAIAAVGNNLITYVFNEMHYPLSKSANIVTNFIGTVFLLSLLGGFLSDS YLGSFWTMLIFGFVELSGFILLAVQAHLPQLRPPACDMMAAAAAEGGCEEASGVKAGI FFAALYLVALGSGCLKPNIIAHGADQFRRGGGGGGDGDGDDGGDGKRLSSYFNAAYFS FCVGELVALTVLVWVQTRSGMDVGFGVSAAAMAVGLVSLVAGVFFYRNKPPQGSIFMP IAKVFVAAVTKRKQVCPSSSSTTAASHAVIPATGAAPVHRINKFRFLDKACVKVQDGH GGGGDGGKESVWPPCTPAEVEQVKVLLCVVPIFACTIVFNTILAQLQTFSVQQGSAMD TRIGGGGGGAAFHIPPASLQAIPYLVLIALVPAYEACFVPAMRRATGVDTGITPLQRI GVGLFAVTFSMVAAALVEAHRRRHAGDGLLSIFWIAPQFLVFGVSEMFTAVGLIEFFY KQSLSGMQAFLTSMTYCSYSFGFYLSSLLVSLVNKVTSGDGAGAGGGGWLSDNDLNKD RLDLFYWLLAGLSLLNFFNYLFWSRWYSKSVETTVQVAGVGGEGGGGEQQEEKGVDEV NMGH&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;3883..4807#3450..3574#1235..1714#439..656#172..310#gcctgaccacacaagcacaaagcaagcactatcaccaaaaggatcagagaagaaactgatatcactatctgatcagtgtagcattgcagcctctcttcttggtaggagtgagctagcacagtgaagagctaattaagccatttgtgtgagcttgcgttgagtgagctctgagctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctaggttcgtttaattagttagttactctcttaatccattatttcttgggtagaaatatgttgcttgatctgatttgttcttgtttgtgtttgtgttttgttggatatcgtttgcattgatgatgattgcagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgtaagaaattgattcatccatccttcatcgtcgatctcttctctttgatatcgcactacgataaaattgacattctttacacctagatgcaactttttgggtccttttttagactgcatttcttggctccttgcaagcaagaacagctgaacaggatcggatcttgtttgtttgtttgtttaatcatgtagggatgttgtttctaggtaggattatatataaaattaaccagataagacacgcgaaagattcagcgactgttcatcgtgccgcgaaaatgatgaggttttgaggaggaggcagcaagttcagcgtaaaggtgagagagagagtggaagaaaatcatggctgcctccatccaatgcggccatgctagctagtgcgccgcccaaaacgccactcgactccacaatatcatttcttggcttttgattttaattttcgagcaggttcattgcatcccatcccatgaccatatccatggcgataattaaccattactgattaatgcgccgccaactttagttagtttagctaataatttttgttgtttttttatttctaatttttcttggcaggggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggtacgtacgttatgccgtacaatacaatcgtcgtacgtgtacggttcgtcatgtacttttatacgctttcttcttttttttttttagagaaaaatatttcttacccggcctctacatccaatcagatatatgcagactttttaaattagaaacttaactctcaaacaatctaatctgaaacttgctactacgattaaactctagatctttaggatacgcttaggacattgcaaccacaattgcatcttcttttcttttctagttggcacattgaaatatcttttgatataattttgacagccatcttttttttaaatgaactctctgtttctaaaatataataacatagtattccattccatgtaacctagtactacgaatctatgtttaattgaactatgaatttagtagatttatagtactgtgttggtgtctattctagtactacgaatttatctcattctgttttagcatcttatattttaggagaggtaggattatttttaatcggacggagtatacaagttggaaacaaatactacatcattgtgttgcatttgcaaaagcagagccaaccaactctgtttttgtgccagctactatattcccctaaaaatattgttgcattcataaatattggaggtcaattttaagctaggtctatgttctctgttcctgcatatggtagttacttgaaaaatcctgaaaatatgataacgtgcattatggtgccatctgtcgctacacaaatatatagtaatatataagtataaaggttagagaaaacatggccgtgggccctagcaatgcgtttctgcactagctaaatagaatatatataatatgtgacaagactaattaatattaagaagcaccataggtagatcaaatttaaaaatgtaaggatgaaaaggcatgtgtatgtacaacaaattaaacaacagaaagtttgaccatacgtatcatcctgattccttgtttggaaagtactactccagatacgctgcttttatatactagtataaaagcaaaaccgcctattttcgttaaatataatgcataatctttatttgatccaaaaaagctacatgatcaaacaggagtttggtacagggactattttatcctcatagtcacctcgtgcactaccacataaaaagcacacgagatcatgggtttacaaccgtttctacattaaacaaaatgaaatgctacctagtttattgccaaatgaagtatttagtcaataaaggaaaagaaagcatatatatgatttactgaatgagataataatgtatatgtatgttgggataaaaaaaataaaactgatgattttaagttacacactggctggcctcctctgccttttctttcttctttctgggacaagttgacaagggctagtggctcagctttatttctctcttttgtgtgggtcaagaattaccactgtctgctgggaggtacctttgattattgatcacagaaatggaggcccattctgttatgcttgtgatacatcaatcatgtttgcatgtgtggggagagacatgtaacatgactgacaatgatctcaggacagcatatatatctttccagcttaattttgtttttagttcttgacttagatcggtataaaccccctaaaactcaattgcacgtgaaaataacaaactctcactcgatcgctgacattttgagctgatggtcggtacatgtgactcaatatgtataggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggtaggtaaaaaaatatttgtaagtggcactgtaatatactacttatttttaaaaaatatatggcaccgttaacttttcgcataatgtttgaccattcgtcttattcaaaaatttaatattaattcataaaaatataagttaagattatattttatttgataataaaacaagtcacaacaagataaaaataaaatttatataatttttttaataagattaatagataaatattatgccaaaagtcaaatggcgtgatatatttcgaaggagtataatagtttactcatttcatgtatgcatgcttacaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactgacatgtgggttcgaaccgcacgtctagtatattaggtggattattaatcatttaacctagtcttaaccttttttttatggtttttccttgtatggaaaaatattagtaagggtggatgtatatgagtccccacctaattaaggagagtgtgtgtgatgtataaattaattaatcaaaagggattaggtgattattacttagtaatgatccctgattaggtcaagtcaaaaggtagtagttctgagtttgactctgcaaggatgcaagagtagtggtatttgggtagggatcaagtgatcaagtacgtatctagtccttttctttaatgataaggagctagtatatatctatgtactttatatatgtagctatcatgtaaatgacaaaaggatttgccataaaagcaaagtactgataatagttggtgcaaaaaatactttcactgttgatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074096.1 RefSeq:Os11g0235200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0235200&diff=176251 2014-06-02T11:57:10Z

<p>Mayamei0424: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> SP1 encodes a putative transporter that belongs to the peptide transporter (PTR) family. SP1 is preferentially expressed in young panicle，especially in the phloem of the branches of young panicle. The sp1-2 mutant showed strikingly reduced panicle and seed size.<br /> <br /> ===Expression===<br /> sp1-2 is one TOS17 insertion mutant. The sp1 mutant plant showed a slight reduction in plant height, owing to the shorted panicle . In comparison with the wild type, the mature panicle length of Sp1-2 was strikingly reduced. Moreover, the seed size of the sp1-2 mutant was smaller than the wild type. In consisted with the smaller seeds, the weight of 1000 seeds was lower of the sp1-2 mutant compared to the wild type (Fig 1d). These results indicated that the SP1 affects not only the panicle size, but also the grain size.<br /> <br /> ===Evolution===<br /> Phylogenetic analysis showed that the SP1 gene is classified into a distinct clade for nitrate transporters, and is closest to AtNRT1:2, an Arabidopsis nitrate transporter<br /> <br /> ==Labs working on this gene==<br /> *State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China,<br /> *State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences,Hangzhou 310006, China,<br /> *Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8652, Japan,<br /> *Research Institute for Bioresources, Okayama University, 2-20-1, Chuo, Kurashiki, Okayama 710-0046, Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os11g0235200|<br /> Description = TGF-beta receptor, type I/II extracellular region family protein|<br /> Version = NM_001074096.1 GI:115484840 GeneID:4350145|<br /> Length = 5257 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0235200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:7176158..7181414|<br /> CDS = 7176608..7177532,7177841..7177965,7179701..7180180,7180759..7180976,7181105..7181243&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;gctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;ANMDVESRQGPKGGDTMAASEVSVDWRGRPCGSRKHGGMKAAVF VLGIQAFEMMAIAAVGNNLITYVFNEMHYPLSKSANIVTNFIGTVFLLSLLGGFLSDS YLGSFWTMLIFGFVELSGFILLAVQAHLPQLRPPACDMMAAAAAEGGCEEASGVKAGI FFAALYLVALGSGCLKPNIIAHGADQFRRGGGGGGDGDGDDGGDGKRLSSYFNAAYFS FCVGELVALTVLVWVQTRSGMDVGFGVSAAAMAVGLVSLVAGVFFYRNKPPQGSIFMP IAKVFVAAVTKRKQVCPSSSSTTAASHAVIPATGAAPVHRINKFRFLDKACVKVQDGH GGGGDGGKESVWPPCTPAEVEQVKVLLCVVPIFACTIVFNTILAQLQTFSVQQGSAMD TRIGGGGGGAAFHIPPASLQAIPYLVLIALVPAYEACFVPAMRRATGVDTGITPLQRI GVGLFAVTFSMVAAALVEAHRRRHAGDGLLSIFWIAPQFLVFGVSEMFTAVGLIEFFY KQSLSGMQAFLTSMTYCSYSFGFYLSSLLVSLVNKVTSGDGAGAGGGGWLSDNDLNKD RLDLFYWLLAGLSLLNFFNYLFWSRWYSKSVETTVQVAGVGGEGGGGEQQEEKGVDEV NMGH&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;3883..4807#3450..3574#1235..1714#439..656#172..310#gcctgaccacacaagcacaaagcaagcactatcaccaaaaggatcagagaagaaactgatatcactatctgatcagtgtagcattgcagcctctcttcttggtaggagtgagctagcacagtgaagagctaattaagccatttgtgtgagcttgcgttgagtgagctctgagctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctaggttcgtttaattagttagttactctcttaatccattatttcttgggtagaaatatgttgcttgatctgatttgttcttgtttgtgtttgtgttttgttggatatcgtttgcattgatgatgattgcagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgtaagaaattgattcatccatccttcatcgtcgatctcttctctttgatatcgcactacgataaaattgacattctttacacctagatgcaactttttgggtccttttttagactgcatttcttggctccttgcaagcaagaacagctgaacaggatcggatcttgtttgtttgtttgtttaatcatgtagggatgttgtttctaggtaggattatatataaaattaaccagataagacacgcgaaagattcagcgactgttcatcgtgccgcgaaaatgatgaggttttgaggaggaggcagcaagttcagcgtaaaggtgagagagagagtggaagaaaatcatggctgcctccatccaatgcggccatgctagctagtgcgccgcccaaaacgccactcgactccacaatatcatttcttggcttttgattttaattttcgagcaggttcattgcatcccatcccatgaccatatccatggcgataattaaccattactgattaatgcgccgccaactttagttagtttagctaataatttttgttgtttttttatttctaatttttcttggcaggggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggtacgtacgttatgccgtacaatacaatcgtcgtacgtgtacggttcgtcatgtacttttatacgctttcttcttttttttttttagagaaaaatatttcttacccggcctctacatccaatcagatatatgcagactttttaaattagaaacttaactctcaaacaatctaatctgaaacttgctactacgattaaactctagatctttaggatacgcttaggacattgcaaccacaattgcatcttcttttcttttctagttggcacattgaaatatcttttgatataattttgacagccatcttttttttaaatgaactctctgtttctaaaatataataacatagtattccattccatgtaacctagtactacgaatctatgtttaattgaactatgaatttagtagatttatagtactgtgttggtgtctattctagtactacgaatttatctcattctgttttagcatcttatattttaggagaggtaggattatttttaatcggacggagtatacaagttggaaacaaatactacatcattgtgttgcatttgcaaaagcagagccaaccaactctgtttttgtgccagctactatattcccctaaaaatattgttgcattcataaatattggaggtcaattttaagctaggtctatgttctctgttcctgcatatggtagttacttgaaaaatcctgaaaatatgataacgtgcattatggtgccatctgtcgctacacaaatatatagtaatatataagtataaaggttagagaaaacatggccgtgggccctagcaatgcgtttctgcactagctaaatagaatatatataatatgtgacaagactaattaatattaagaagcaccataggtagatcaaatttaaaaatgtaaggatgaaaaggcatgtgtatgtacaacaaattaaacaacagaaagtttgaccatacgtatcatcctgattccttgtttggaaagtactactccagatacgctgcttttatatactagtataaaagcaaaaccgcctattttcgttaaatataatgcataatctttatttgatccaaaaaagctacatgatcaaacaggagtttggtacagggactattttatcctcatagtcacctcgtgcactaccacataaaaagcacacgagatcatgggtttacaaccgtttctacattaaacaaaatgaaatgctacctagtttattgccaaatgaagtatttagtcaataaaggaaaagaaagcatatatatgatttactgaatgagataataatgtatatgtatgttgggataaaaaaaataaaactgatgattttaagttacacactggctggcctcctctgccttttctttcttctttctgggacaagttgacaagggctagtggctcagctttatttctctcttttgtgtgggtcaagaattaccactgtctgctgggaggtacctttgattattgatcacagaaatggaggcccattctgttatgcttgtgatacatcaatcatgtttgcatgtgtggggagagacatgtaacatgactgacaatgatctcaggacagcatatatatctttccagcttaattttgtttttagttcttgacttagatcggtataaaccccctaaaactcaattgcacgtgaaaataacaaactctcactcgatcgctgacattttgagctgatggtcggtacatgtgactcaatatgtataggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggtaggtaaaaaaatatttgtaagtggcactgtaatatactacttatttttaaaaaatatatggcaccgttaacttttcgcataatgtttgaccattcgtcttattcaaaaatttaatattaattcataaaaatataagttaagattatattttatttgataataaaacaagtcacaacaagataaaaataaaatttatataatttttttaataagattaatagataaatattatgccaaaagtcaaatggcgtgatatatttcgaaggagtataatagtttactcatttcatgtatgcatgcttacaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactgacatgtgggttcgaaccgcacgtctagtatattaggtggattattaatcatttaacctagtcttaaccttttttttatggtttttccttgtatggaaaaatattagtaagggtggatgtatatgagtccccacctaattaaggagagtgtgtgtgatgtataaattaattaatcaaaagggattaggtgattattacttagtaatgatccctgattaggtcaagtcaaaaggtagtagttctgagtttgactctgcaaggatgcaagagtagtggtatttgggtagggatcaagtgatcaagtacgtatctagtccttttctttaatgataaggagctagtatatatctatgtactttatatatgtagctatcatgtaaatgacaaaaggatttgccataaaagcaaagtactgataatagttggtgcaaaaaatactttcactgttgatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074096.1 RefSeq:Os11g0235200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0235200&diff=176248 2014-06-02T11:56:43Z

<p>Mayamei0424: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> SP1 encodes a putative transporter that belongs to the peptide transporter (PTR) family. SP1 is preferentially expressed in young panicle，especially in the phloem of the branches of young panicle. The sp1-2 mutant showed strikingly reduced panicle and seed size.<br /> <br /> ===Expression===<br /> sp1-2 is one TOS17 insertion mutant. The sp1 mutant plant showed a slight reduction in plant height, owing to the shorted panicle . In comparison with the wild type, the mature panicle length of Sp1-2 was strikingly reduced. Moreover, the seed size of the sp1-2 mutant was smaller than the wild type. In consisted with the smaller seeds, the weight of 1000 seeds was lower of the sp1-2 mutant compared to the wild type (Fig 1d). These results indicated that the SP1 affects not only the panicle size, but also the grain size.<br /> <br /> ===Evolution===<br /> Phylogenetic analysis showed that the SP1 gene is classified into a distinct clade for nitrate transporters, and is closest to AtNRT1:2, an Arabidopsis nitrate transporter<br /> <br /> ==Labs working on this gene==<br /> *State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental<br /> Biology, Chinese Academy of Sciences, Beijing 100101, China,<br /> *State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences,<br /> Hangzhou 310006, China,<br /> *Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8652, Japan, and<br /> *Research Institute for Bioresources, Okayama University, 2-20-1, Chuo, Kurashiki, Okayama 710-0046, Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os11g0235200|<br /> Description = TGF-beta receptor, type I/II extracellular region family protein|<br /> Version = NM_001074096.1 GI:115484840 GeneID:4350145|<br /> Length = 5257 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0235200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:7176158..7181414|<br /> CDS = 7176608..7177532,7177841..7177965,7179701..7180180,7180759..7180976,7181105..7181243&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;gctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;ANMDVESRQGPKGGDTMAASEVSVDWRGRPCGSRKHGGMKAAVF VLGIQAFEMMAIAAVGNNLITYVFNEMHYPLSKSANIVTNFIGTVFLLSLLGGFLSDS YLGSFWTMLIFGFVELSGFILLAVQAHLPQLRPPACDMMAAAAAEGGCEEASGVKAGI FFAALYLVALGSGCLKPNIIAHGADQFRRGGGGGGDGDGDDGGDGKRLSSYFNAAYFS FCVGELVALTVLVWVQTRSGMDVGFGVSAAAMAVGLVSLVAGVFFYRNKPPQGSIFMP IAKVFVAAVTKRKQVCPSSSSTTAASHAVIPATGAAPVHRINKFRFLDKACVKVQDGH GGGGDGGKESVWPPCTPAEVEQVKVLLCVVPIFACTIVFNTILAQLQTFSVQQGSAMD TRIGGGGGGAAFHIPPASLQAIPYLVLIALVPAYEACFVPAMRRATGVDTGITPLQRI GVGLFAVTFSMVAAALVEAHRRRHAGDGLLSIFWIAPQFLVFGVSEMFTAVGLIEFFY KQSLSGMQAFLTSMTYCSYSFGFYLSSLLVSLVNKVTSGDGAGAGGGGWLSDNDLNKD RLDLFYWLLAGLSLLNFFNYLFWSRWYSKSVETTVQVAGVGGEGGGGEQQEEKGVDEV NMGH&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;3883..4807#3450..3574#1235..1714#439..656#172..310#gcctgaccacacaagcacaaagcaagcactatcaccaaaaggatcagagaagaaactgatatcactatctgatcagtgtagcattgcagcctctcttcttggtaggagtgagctagcacagtgaagagctaattaagccatttgtgtgagcttgcgttgagtgagctctgagctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctaggttcgtttaattagttagttactctcttaatccattatttcttgggtagaaatatgttgcttgatctgatttgttcttgtttgtgtttgtgttttgttggatatcgtttgcattgatgatgattgcagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgtaagaaattgattcatccatccttcatcgtcgatctcttctctttgatatcgcactacgataaaattgacattctttacacctagatgcaactttttgggtccttttttagactgcatttcttggctccttgcaagcaagaacagctgaacaggatcggatcttgtttgtttgtttgtttaatcatgtagggatgttgtttctaggtaggattatatataaaattaaccagataagacacgcgaaagattcagcgactgttcatcgtgccgcgaaaatgatgaggttttgaggaggaggcagcaagttcagcgtaaaggtgagagagagagtggaagaaaatcatggctgcctccatccaatgcggccatgctagctagtgcgccgcccaaaacgccactcgactccacaatatcatttcttggcttttgattttaattttcgagcaggttcattgcatcccatcccatgaccatatccatggcgataattaaccattactgattaatgcgccgccaactttagttagtttagctaataatttttgttgtttttttatttctaatttttcttggcaggggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggtacgtacgttatgccgtacaatacaatcgtcgtacgtgtacggttcgtcatgtacttttatacgctttcttcttttttttttttagagaaaaatatttcttacccggcctctacatccaatcagatatatgcagactttttaaattagaaacttaactctcaaacaatctaatctgaaacttgctactacgattaaactctagatctttaggatacgcttaggacattgcaaccacaattgcatcttcttttcttttctagttggcacattgaaatatcttttgatataattttgacagccatcttttttttaaatgaactctctgtttctaaaatataataacatagtattccattccatgtaacctagtactacgaatctatgtttaattgaactatgaatttagtagatttatagtactgtgttggtgtctattctagtactacgaatttatctcattctgttttagcatcttatattttaggagaggtaggattatttttaatcggacggagtatacaagttggaaacaaatactacatcattgtgttgcatttgcaaaagcagagccaaccaactctgtttttgtgccagctactatattcccctaaaaatattgttgcattcataaatattggaggtcaattttaagctaggtctatgttctctgttcctgcatatggtagttacttgaaaaatcctgaaaatatgataacgtgcattatggtgccatctgtcgctacacaaatatatagtaatatataagtataaaggttagagaaaacatggccgtgggccctagcaatgcgtttctgcactagctaaatagaatatatataatatgtgacaagactaattaatattaagaagcaccataggtagatcaaatttaaaaatgtaaggatgaaaaggcatgtgtatgtacaacaaattaaacaacagaaagtttgaccatacgtatcatcctgattccttgtttggaaagtactactccagatacgctgcttttatatactagtataaaagcaaaaccgcctattttcgttaaatataatgcataatctttatttgatccaaaaaagctacatgatcaaacaggagtttggtacagggactattttatcctcatagtcacctcgtgcactaccacataaaaagcacacgagatcatgggtttacaaccgtttctacattaaacaaaatgaaatgctacctagtttattgccaaatgaagtatttagtcaataaaggaaaagaaagcatatatatgatttactgaatgagataataatgtatatgtatgttgggataaaaaaaataaaactgatgattttaagttacacactggctggcctcctctgccttttctttcttctttctgggacaagttgacaagggctagtggctcagctttatttctctcttttgtgtgggtcaagaattaccactgtctgctgggaggtacctttgattattgatcacagaaatggaggcccattctgttatgcttgtgatacatcaatcatgtttgcatgtgtggggagagacatgtaacatgactgacaatgatctcaggacagcatatatatctttccagcttaattttgtttttagttcttgacttagatcggtataaaccccctaaaactcaattgcacgtgaaaataacaaactctcactcgatcgctgacattttgagctgatggtcggtacatgtgactcaatatgtataggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggtaggtaaaaaaatatttgtaagtggcactgtaatatactacttatttttaaaaaatatatggcaccgttaacttttcgcataatgtttgaccattcgtcttattcaaaaatttaatattaattcataaaaatataagttaagattatattttatttgataataaaacaagtcacaacaagataaaaataaaatttatataatttttttaataagattaatagataaatattatgccaaaagtcaaatggcgtgatatatttcgaaggagtataatagtttactcatttcatgtatgcatgcttacaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactgacatgtgggttcgaaccgcacgtctagtatattaggtggattattaatcatttaacctagtcttaaccttttttttatggtttttccttgtatggaaaaatattagtaagggtggatgtatatgagtccccacctaattaaggagagtgtgtgtgatgtataaattaattaatcaaaagggattaggtgattattacttagtaatgatccctgattaggtcaagtcaaaaggtagtagttctgagtttgactctgcaaggatgcaagagtagtggtatttgggtagggatcaagtgatcaagtacgtatctagtccttttctttaatgataaggagctagtatatatctatgtactttatatatgtagctatcatgtaaatgacaaaaggatttgccataaaagcaaagtactgataatagttggtgcaaaaaatactttcactgttgatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074096.1 RefSeq:Os11g0235200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0235200&diff=176247 2014-06-02T11:55:25Z

<p>Mayamei0424: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> SP1 encodes a putative transporter that belongs to the peptide transporter (PTR) family. SP1 is preferentially expressed in young panicle，especially in the phloem of the branches of young panicle. The sp1-2 mutant showed strikingly reduced panicle and seed size.<br /> <br /> ===Expression===<br /> sp1-2 is one TOS17 insertion mutant. The sp1 mutant plant showed a slight reduction in plant height, owing to the shorted panicle . In comparison with the wild type, the mature panicle length of Sp1-2 was strikingly reduced. Moreover, the seed size of the sp1-2 mutant was smaller than the wild type. In consisted with the smaller seeds, the weight of 1000 seeds was lower of the sp1-2 mutant compared to the wild type (Fig 1d). These results indicated that the SP1 affects not only the panicle size, but also the grain size.<br /> <br /> ===Evolution===<br /> Phylogenetic analysis showed that the SP1 gene is classified into a distinct clade for nitrate transporters, and is closest to AtNRT1:2, an Arabidopsis nitrate transporter<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os11g0235200|<br /> Description = TGF-beta receptor, type I/II extracellular region family protein|<br /> Version = NM_001074096.1 GI:115484840 GeneID:4350145|<br /> Length = 5257 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0235200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:7176158..7181414|<br /> CDS = 7176608..7177532,7177841..7177965,7179701..7180180,7180759..7180976,7181105..7181243&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;gctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;ANMDVESRQGPKGGDTMAASEVSVDWRGRPCGSRKHGGMKAAVF VLGIQAFEMMAIAAVGNNLITYVFNEMHYPLSKSANIVTNFIGTVFLLSLLGGFLSDS YLGSFWTMLIFGFVELSGFILLAVQAHLPQLRPPACDMMAAAAAEGGCEEASGVKAGI FFAALYLVALGSGCLKPNIIAHGADQFRRGGGGGGDGDGDDGGDGKRLSSYFNAAYFS FCVGELVALTVLVWVQTRSGMDVGFGVSAAAMAVGLVSLVAGVFFYRNKPPQGSIFMP IAKVFVAAVTKRKQVCPSSSSTTAASHAVIPATGAAPVHRINKFRFLDKACVKVQDGH GGGGDGGKESVWPPCTPAEVEQVKVLLCVVPIFACTIVFNTILAQLQTFSVQQGSAMD TRIGGGGGGAAFHIPPASLQAIPYLVLIALVPAYEACFVPAMRRATGVDTGITPLQRI GVGLFAVTFSMVAAALVEAHRRRHAGDGLLSIFWIAPQFLVFGVSEMFTAVGLIEFFY KQSLSGMQAFLTSMTYCSYSFGFYLSSLLVSLVNKVTSGDGAGAGGGGWLSDNDLNKD RLDLFYWLLAGLSLLNFFNYLFWSRWYSKSVETTVQVAGVGGEGGGGEQQEEKGVDEV NMGH&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;3883..4807#3450..3574#1235..1714#439..656#172..310#gcctgaccacacaagcacaaagcaagcactatcaccaaaaggatcagagaagaaactgatatcactatctgatcagtgtagcattgcagcctctcttcttggtaggagtgagctagcacagtgaagagctaattaagccatttgtgtgagcttgcgttgagtgagctctgagctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctaggttcgtttaattagttagttactctcttaatccattatttcttgggtagaaatatgttgcttgatctgatttgttcttgtttgtgtttgtgttttgttggatatcgtttgcattgatgatgattgcagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgtaagaaattgattcatccatccttcatcgtcgatctcttctctttgatatcgcactacgataaaattgacattctttacacctagatgcaactttttgggtccttttttagactgcatttcttggctccttgcaagcaagaacagctgaacaggatcggatcttgtttgtttgtttgtttaatcatgtagggatgttgtttctaggtaggattatatataaaattaaccagataagacacgcgaaagattcagcgactgttcatcgtgccgcgaaaatgatgaggttttgaggaggaggcagcaagttcagcgtaaaggtgagagagagagtggaagaaaatcatggctgcctccatccaatgcggccatgctagctagtgcgccgcccaaaacgccactcgactccacaatatcatttcttggcttttgattttaattttcgagcaggttcattgcatcccatcccatgaccatatccatggcgataattaaccattactgattaatgcgccgccaactttagttagtttagctaataatttttgttgtttttttatttctaatttttcttggcaggggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggtacgtacgttatgccgtacaatacaatcgtcgtacgtgtacggttcgtcatgtacttttatacgctttcttcttttttttttttagagaaaaatatttcttacccggcctctacatccaatcagatatatgcagactttttaaattagaaacttaactctcaaacaatctaatctgaaacttgctactacgattaaactctagatctttaggatacgcttaggacattgcaaccacaattgcatcttcttttcttttctagttggcacattgaaatatcttttgatataattttgacagccatcttttttttaaatgaactctctgtttctaaaatataataacatagtattccattccatgtaacctagtactacgaatctatgtttaattgaactatgaatttagtagatttatagtactgtgttggtgtctattctagtactacgaatttatctcattctgttttagcatcttatattttaggagaggtaggattatttttaatcggacggagtatacaagttggaaacaaatactacatcattgtgttgcatttgcaaaagcagagccaaccaactctgtttttgtgccagctactatattcccctaaaaatattgttgcattcataaatattggaggtcaattttaagctaggtctatgttctctgttcctgcatatggtagttacttgaaaaatcctgaaaatatgataacgtgcattatggtgccatctgtcgctacacaaatatatagtaatatataagtataaaggttagagaaaacatggccgtgggccctagcaatgcgtttctgcactagctaaatagaatatatataatatgtgacaagactaattaatattaagaagcaccataggtagatcaaatttaaaaatgtaaggatgaaaaggcatgtgtatgtacaacaaattaaacaacagaaagtttgaccatacgtatcatcctgattccttgtttggaaagtactactccagatacgctgcttttatatactagtataaaagcaaaaccgcctattttcgttaaatataatgcataatctttatttgatccaaaaaagctacatgatcaaacaggagtttggtacagggactattttatcctcatagtcacctcgtgcactaccacataaaaagcacacgagatcatgggtttacaaccgtttctacattaaacaaaatgaaatgctacctagtttattgccaaatgaagtatttagtcaataaaggaaaagaaagcatatatatgatttactgaatgagataataatgtatatgtatgttgggataaaaaaaataaaactgatgattttaagttacacactggctggcctcctctgccttttctttcttctttctgggacaagttgacaagggctagtggctcagctttatttctctcttttgtgtgggtcaagaattaccactgtctgctgggaggtacctttgattattgatcacagaaatggaggcccattctgttatgcttgtgatacatcaatcatgtttgcatgtgtggggagagacatgtaacatgactgacaatgatctcaggacagcatatatatctttccagcttaattttgtttttagttcttgacttagatcggtataaaccccctaaaactcaattgcacgtgaaaataacaaactctcactcgatcgctgacattttgagctgatggtcggtacatgtgactcaatatgtataggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggtaggtaaaaaaatatttgtaagtggcactgtaatatactacttatttttaaaaaatatatggcaccgttaacttttcgcataatgtttgaccattcgtcttattcaaaaatttaatattaattcataaaaatataagttaagattatattttatttgataataaaacaagtcacaacaagataaaaataaaatttatataatttttttaataagattaatagataaatattatgccaaaagtcaaatggcgtgatatatttcgaaggagtataatagtttactcatttcatgtatgcatgcttacaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactgacatgtgggttcgaaccgcacgtctagtatattaggtggattattaatcatttaacctagtcttaaccttttttttatggtttttccttgtatggaaaaatattagtaagggtggatgtatatgagtccccacctaattaaggagagtgtgtgtgatgtataaattaattaatcaaaagggattaggtgattattacttagtaatgatccctgattaggtcaagtcaaaaggtagtagttctgagtttgactctgcaaggatgcaagagtagtggtatttgggtagggatcaagtgatcaagtacgtatctagtccttttctttaatgataaggagctagtatatatctatgtactttatatatgtagctatcatgtaaatgacaaaaggatttgccataaaagcaaagtactgataatagttggtgcaaaaaatactttcactgttgatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074096.1 RefSeq:Os11g0235200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0235200&diff=176245 2014-06-02T11:54:40Z

<p>Mayamei0424: /* Evolution */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> SP1 encodes a putative transporter that belongs to the peptide transporter (PTR) family. SP1 is preferentially expressed in young panicle，especially in the phloem of the branches of young panicle. The sp1-2 mutant showed strikingly reduced panicle and seed size.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Phylogenetic analysis showed that the SP1 gene is classified into a distinct clade for nitrate transporters, and is closest to AtNRT1:2, an Arabidopsis nitrate transporter<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os11g0235200|<br /> Description = TGF-beta receptor, type I/II extracellular region family protein|<br /> Version = NM_001074096.1 GI:115484840 GeneID:4350145|<br /> Length = 5257 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0235200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:7176158..7181414|<br /> CDS = 7176608..7177532,7177841..7177965,7179701..7180180,7180759..7180976,7181105..7181243&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;gctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;ANMDVESRQGPKGGDTMAASEVSVDWRGRPCGSRKHGGMKAAVF VLGIQAFEMMAIAAVGNNLITYVFNEMHYPLSKSANIVTNFIGTVFLLSLLGGFLSDS YLGSFWTMLIFGFVELSGFILLAVQAHLPQLRPPACDMMAAAAAEGGCEEASGVKAGI FFAALYLVALGSGCLKPNIIAHGADQFRRGGGGGGDGDGDDGGDGKRLSSYFNAAYFS FCVGELVALTVLVWVQTRSGMDVGFGVSAAAMAVGLVSLVAGVFFYRNKPPQGSIFMP IAKVFVAAVTKRKQVCPSSSSTTAASHAVIPATGAAPVHRINKFRFLDKACVKVQDGH GGGGDGGKESVWPPCTPAEVEQVKVLLCVVPIFACTIVFNTILAQLQTFSVQQGSAMD TRIGGGGGGAAFHIPPASLQAIPYLVLIALVPAYEACFVPAMRRATGVDTGITPLQRI GVGLFAVTFSMVAAALVEAHRRRHAGDGLLSIFWIAPQFLVFGVSEMFTAVGLIEFFY KQSLSGMQAFLTSMTYCSYSFGFYLSSLLVSLVNKVTSGDGAGAGGGGWLSDNDLNKD RLDLFYWLLAGLSLLNFFNYLFWSRWYSKSVETTVQVAGVGGEGGGGEQQEEKGVDEV NMGH&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;3883..4807#3450..3574#1235..1714#439..656#172..310#gcctgaccacacaagcacaaagcaagcactatcaccaaaaggatcagagaagaaactgatatcactatctgatcagtgtagcattgcagcctctcttcttggtaggagtgagctagcacagtgaagagctaattaagccatttgtgtgagcttgcgttgagtgagctctgagctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctaggttcgtttaattagttagttactctcttaatccattatttcttgggtagaaatatgttgcttgatctgatttgttcttgtttgtgtttgtgttttgttggatatcgtttgcattgatgatgattgcagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgtaagaaattgattcatccatccttcatcgtcgatctcttctctttgatatcgcactacgataaaattgacattctttacacctagatgcaactttttgggtccttttttagactgcatttcttggctccttgcaagcaagaacagctgaacaggatcggatcttgtttgtttgtttgtttaatcatgtagggatgttgtttctaggtaggattatatataaaattaaccagataagacacgcgaaagattcagcgactgttcatcgtgccgcgaaaatgatgaggttttgaggaggaggcagcaagttcagcgtaaaggtgagagagagagtggaagaaaatcatggctgcctccatccaatgcggccatgctagctagtgcgccgcccaaaacgccactcgactccacaatatcatttcttggcttttgattttaattttcgagcaggttcattgcatcccatcccatgaccatatccatggcgataattaaccattactgattaatgcgccgccaactttagttagtttagctaataatttttgttgtttttttatttctaatttttcttggcaggggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggtacgtacgttatgccgtacaatacaatcgtcgtacgtgtacggttcgtcatgtacttttatacgctttcttcttttttttttttagagaaaaatatttcttacccggcctctacatccaatcagatatatgcagactttttaaattagaaacttaactctcaaacaatctaatctgaaacttgctactacgattaaactctagatctttaggatacgcttaggacattgcaaccacaattgcatcttcttttcttttctagttggcacattgaaatatcttttgatataattttgacagccatcttttttttaaatgaactctctgtttctaaaatataataacatagtattccattccatgtaacctagtactacgaatctatgtttaattgaactatgaatttagtagatttatagtactgtgttggtgtctattctagtactacgaatttatctcattctgttttagcatcttatattttaggagaggtaggattatttttaatcggacggagtatacaagttggaaacaaatactacatcattgtgttgcatttgcaaaagcagagccaaccaactctgtttttgtgccagctactatattcccctaaaaatattgttgcattcataaatattggaggtcaattttaagctaggtctatgttctctgttcctgcatatggtagttacttgaaaaatcctgaaaatatgataacgtgcattatggtgccatctgtcgctacacaaatatatagtaatatataagtataaaggttagagaaaacatggccgtgggccctagcaatgcgtttctgcactagctaaatagaatatatataatatgtgacaagactaattaatattaagaagcaccataggtagatcaaatttaaaaatgtaaggatgaaaaggcatgtgtatgtacaacaaattaaacaacagaaagtttgaccatacgtatcatcctgattccttgtttggaaagtactactccagatacgctgcttttatatactagtataaaagcaaaaccgcctattttcgttaaatataatgcataatctttatttgatccaaaaaagctacatgatcaaacaggagtttggtacagggactattttatcctcatagtcacctcgtgcactaccacataaaaagcacacgagatcatgggtttacaaccgtttctacattaaacaaaatgaaatgctacctagtttattgccaaatgaagtatttagtcaataaaggaaaagaaagcatatatatgatttactgaatgagataataatgtatatgtatgttgggataaaaaaaataaaactgatgattttaagttacacactggctggcctcctctgccttttctttcttctttctgggacaagttgacaagggctagtggctcagctttatttctctcttttgtgtgggtcaagaattaccactgtctgctgggaggtacctttgattattgatcacagaaatggaggcccattctgttatgcttgtgatacatcaatcatgtttgcatgtgtggggagagacatgtaacatgactgacaatgatctcaggacagcatatatatctttccagcttaattttgtttttagttcttgacttagatcggtataaaccccctaaaactcaattgcacgtgaaaataacaaactctcactcgatcgctgacattttgagctgatggtcggtacatgtgactcaatatgtataggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggtaggtaaaaaaatatttgtaagtggcactgtaatatactacttatttttaaaaaatatatggcaccgttaacttttcgcataatgtttgaccattcgtcttattcaaaaatttaatattaattcataaaaatataagttaagattatattttatttgataataaaacaagtcacaacaagataaaaataaaatttatataatttttttaataagattaatagataaatattatgccaaaagtcaaatggcgtgatatatttcgaaggagtataatagtttactcatttcatgtatgcatgcttacaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactgacatgtgggttcgaaccgcacgtctagtatattaggtggattattaatcatttaacctagtcttaaccttttttttatggtttttccttgtatggaaaaatattagtaagggtggatgtatatgagtccccacctaattaaggagagtgtgtgtgatgtataaattaattaatcaaaagggattaggtgattattacttagtaatgatccctgattaggtcaagtcaaaaggtagtagttctgagtttgactctgcaaggatgcaagagtagtggtatttgggtagggatcaagtgatcaagtacgtatctagtccttttctttaatgataaggagctagtatatatctatgtactttatatatgtagctatcatgtaaatgacaaaaggatttgccataaaagcaaagtactgataatagttggtgcaaaaaatactttcactgttgatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074096.1 RefSeq:Os11g0235200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os11g0235200&diff=176242 2014-06-02T11:53:52Z

<p>Mayamei0424: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> SP1 encodes a putative transporter that belongs to the peptide transporter (PTR) family. SP1 is preferentially expressed in young panicle，especially in the phloem of the branches of young panicle. The sp1-2 mutant showed strikingly reduced panicle and seed size.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os11g0235200|<br /> Description = TGF-beta receptor, type I/II extracellular region family protein|<br /> Version = NM_001074096.1 GI:115484840 GeneID:4350145|<br /> Length = 5257 bp|<br /> Definition = Oryza sativa Japonica Group Os11g0235200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|<br /> AP = Chromosome 11:7176158..7181414|<br /> CDS = 7176608..7177532,7177841..7177965,7179701..7180180,7180759..7180976,7181105..7181243&lt;br&gt;|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008404:7176158..7181414<br /> source=RiceChromosome11<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;gctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;ANMDVESRQGPKGGDTMAASEVSVDWRGRPCGSRKHGGMKAAVF VLGIQAFEMMAIAAVGNNLITYVFNEMHYPLSKSANIVTNFIGTVFLLSLLGGFLSDS YLGSFWTMLIFGFVELSGFILLAVQAHLPQLRPPACDMMAAAAAEGGCEEASGVKAGI FFAALYLVALGSGCLKPNIIAHGADQFRRGGGGGGDGDGDDGGDGKRLSSYFNAAYFS FCVGELVALTVLVWVQTRSGMDVGFGVSAAAMAVGLVSLVAGVFFYRNKPPQGSIFMP IAKVFVAAVTKRKQVCPSSSSTTAASHAVIPATGAAPVHRINKFRFLDKACVKVQDGH GGGGDGGKESVWPPCTPAEVEQVKVLLCVVPIFACTIVFNTILAQLQTFSVQQGSAMD TRIGGGGGGAAFHIPPASLQAIPYLVLIALVPAYEACFVPAMRRATGVDTGITPLQRI GVGLFAVTFSMVAAALVEAHRRRHAGDGLLSIFWIAPQFLVFGVSEMFTAVGLIEFFY KQSLSGMQAFLTSMTYCSYSFGFYLSSLLVSLVNKVTSGDGAGAGGGGWLSDNDLNKD RLDLFYWLLAGLSLLNFFNYLFWSRWYSKSVETTVQVAGVGGEGGGGEQQEEKGVDEV NMGH&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;3883..4807#3450..3574#1235..1714#439..656#172..310#gcctgaccacacaagcacaaagcaagcactatcaccaaaaggatcagagaagaaactgatatcactatctgatcagtgtagcattgcagcctctcttcttggtaggagtgagctagcacagtgaagagctaattaagccatttgtgtgagcttgcgttgagtgagctctgagctaatatggatgttgagtcaaggcaaggccccaagggtggtgacaccatggctgcctccgaggtctccgtggactggaggggcaggccttgcggctctcgcaagcatggcggcatgaaagcggccgtctttgtgctaggttcgtttaattagttagttactctcttaatccattatttcttgggtagaaatatgttgcttgatctgatttgttcttgtttgtgtttgtgttttgttggatatcgtttgcattgatgatgattgcagggatccaggcgttcgagatgatggcgatcgcggcggtggggaacaacctgatcacctacgtcttcaacgagatgcactacccgctgtcaaagtcggccaacatcgtgaccaacttcattggcaccgtcttcctcctctccctcctcggtggcttcctctccgactcctacctcggaagcttctggaccatgctcatcttcggcttcgtcgagctctccgtaagaaattgattcatccatccttcatcgtcgatctcttctctttgatatcgcactacgataaaattgacattctttacacctagatgcaactttttgggtccttttttagactgcatttcttggctccttgcaagcaagaacagctgaacaggatcggatcttgtttgtttgtttgtttaatcatgtagggatgttgtttctaggtaggattatatataaaattaaccagataagacacgcgaaagattcagcgactgttcatcgtgccgcgaaaatgatgaggttttgaggaggaggcagcaagttcagcgtaaaggtgagagagagagtggaagaaaatcatggctgcctccatccaatgcggccatgctagctagtgcgccgcccaaaacgccactcgactccacaatatcatttcttggcttttgattttaattttcgagcaggttcattgcatcccatcccatgaccatatccatggcgataattaaccattactgattaatgcgccgccaactttagttagtttagctaataatttttgttgtttttttatttctaatttttcttggcaggggttcatactgctggccgtgcaggcgcacctgccgcagctgcggccgccggcgtgcgacatgatggcggcggcggcggcggaggggggatgcgaggaggcgagcggggtgaaggcggggatcttcttcgcggcgctgtacctggtggcgctggggagcgggtgcctcaagcccaacatcatcgcgcacggcgccgaccagttccggcgcggcggcggcggcggcggcgacggcgatggcgacgatggcggcgacgggaagcggctgtcgagctacttcaacgcggcctacttcagcttctgcgtgggggagctggtggcgctgacggtgctggtgtgggtgcagacgaggtcggggatggacgtggggttcggggtgtcggcggccgccatggccgtggggctggtcagcctcgtcgccggcgtcttcttctaccggaacaagccgccgcagggcagcatcttcatgcccatcgccaaggtacgtacgttatgccgtacaatacaatcgtcgtacgtgtacggttcgtcatgtacttttatacgctttcttcttttttttttttagagaaaaatatttcttacccggcctctacatccaatcagatatatgcagactttttaaattagaaacttaactctcaaacaatctaatctgaaacttgctactacgattaaactctagatctttaggatacgcttaggacattgcaaccacaattgcatcttcttttcttttctagttggcacattgaaatatcttttgatataattttgacagccatcttttttttaaatgaactctctgtttctaaaatataataacatagtattccattccatgtaacctagtactacgaatctatgtttaattgaactatgaatttagtagatttatagtactgtgttggtgtctattctagtactacgaatttatctcattctgttttagcatcttatattttaggagaggtaggattatttttaatcggacggagtatacaagttggaaacaaatactacatcattgtgttgcatttgcaaaagcagagccaaccaactctgtttttgtgccagctactatattcccctaaaaatattgttgcattcataaatattggaggtcaattttaagctaggtctatgttctctgttcctgcatatggtagttacttgaaaaatcctgaaaatatgataacgtgcattatggtgccatctgtcgctacacaaatatatagtaatatataagtataaaggttagagaaaacatggccgtgggccctagcaatgcgtttctgcactagctaaatagaatatatataatatgtgacaagactaattaatattaagaagcaccataggtagatcaaatttaaaaatgtaaggatgaaaaggcatgtgtatgtacaacaaattaaacaacagaaagtttgaccatacgtatcatcctgattccttgtttggaaagtactactccagatacgctgcttttatatactagtataaaagcaaaaccgcctattttcgttaaatataatgcataatctttatttgatccaaaaaagctacatgatcaaacaggagtttggtacagggactattttatcctcatagtcacctcgtgcactaccacataaaaagcacacgagatcatgggtttacaaccgtttctacattaaacaaaatgaaatgctacctagtttattgccaaatgaagtatttagtcaataaaggaaaagaaagcatatatatgatttactgaatgagataataatgtatatgtatgttgggataaaaaaaataaaactgatgattttaagttacacactggctggcctcctctgccttttctttcttctttctgggacaagttgacaagggctagtggctcagctttatttctctcttttgtgtgggtcaagaattaccactgtctgctgggaggtacctttgattattgatcacagaaatggaggcccattctgttatgcttgtgatacatcaatcatgtttgcatgtgtggggagagacatgtaacatgactgacaatgatctcaggacagcatatatatctttccagcttaattttgtttttagttcttgacttagatcggtataaaccccctaaaactcaattgcacgtgaaaataacaaactctcactcgatcgctgacattttgagctgatggtcggtacatgtgactcaatatgtataggttttcgtcgcggcggtaaccaagaggaaacaagtgtgcccatcatcatcgtcgacgacagccgcctcgcatgctgtgatccccgccaccggtgccgcccctgtccaccgcatcaacaaattcaggtaggtaaaaaaatatttgtaagtggcactgtaatatactacttatttttaaaaaatatatggcaccgttaacttttcgcataatgtttgaccattcgtcttattcaaaaatttaatattaattcataaaaatataagttaagattatattttatttgataataaaacaagtcacaacaagataaaaataaaatttatataatttttttaataagattaatagataaatattatgccaaaagtcaaatggcgtgatatatttcgaaggagtataatagtttactcatttcatgtatgcatgcttacaggttcttggacaaggcgtgcgtgaaggttcaggacggccatggcggcggcggcgacggggggaaggagagcgtgtggccgccgtgcacgccggcggaggtggagcaggtgaaggtgctactgtgcgtggtgcccatcttcgcgtgcaccatcgtcttcaacaccatcctggcgcagctgcagacgttctccgtgcagcagggctccgccatggacacccgcatcggcggcggcggcggcggcgccgccttccacatcccgccggcgtcgctgcaggccatcccctacctcgtcctcatcgcgctggtgccggcgtacgaggcgtgcttcgtgccggccatgcgccgcgccacgggcgtggacaccggcatcaccccgctccagcggatcggggtcggcctcttcgccgtcaccttctccatggtcgccgccgcgctcgtcgaggcacaccgccgccgccacgccggcgacgggctcctctccatcttctggatcgcgccgcagttcctggtgttcggcgtgtccgagatgttcaccgccgtggggctcatcgagttcttctacaagcagtcgctctccggcatgcaggccttcctcacctcaatgacctactgctcctactccttcggcttctacctcagctccctcctcgtctcgctcgtcaacaaggtcacctcgggcgacggcgccggcgccggcggcggcggctggctcagcgacaacgacctcaacaaggacaggctcgacctcttctactggctcctcgccgggctcagcctcctcaacttcttcaactacctcttctggtcgaggtggtactccaagagtgtggagacgaccgtgcaggtcgccggagttggaggagaaggcggcggcggcgagcagcaggaggagaagggtgttgacgaggtcaacatgggtcactgacatgtgggttcgaaccgcacgtctagtatattaggtggattattaatcatttaacctagtcttaaccttttttttatggtttttccttgtatggaaaaatattagtaagggtggatgtatatgagtccccacctaattaaggagagtgtgtgtgatgtataaattaattaatcaaaagggattaggtgattattacttagtaatgatccctgattaggtcaagtcaaaaggtagtagttctgagtttgactctgcaaggatgcaagagtagtggtatttgggtagggatcaagtgatcaagtacgtatctagtccttttctttaatgataaggagctagtatatatctatgtactttatatatgtagctatcatgtaaatgacaaaaggatttgccataaaagcaaagtactgataatagttggtgcaaaaaatactttcactgttgatc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074096.1 RefSeq:Os11g0235200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 11]]<br /> [[Category:Chromosome 11]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os04g0473900&diff=175260 2014-06-01T01:56:43Z

<p>Mayamei0424: /* Mutation */</p> <hr /> <div>''DWA1'' encodes an enzymatic megaprotein conserved in vascular plants，and ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''DWA1'' encodes an enzymatic megaprotein (2,391 aa in length) conserved in vascular plants, including an oxidoreductase-like domain, a prokaryotic nonribosomal peptide synthetase-like module, an AMP-binding domain, and an allene oxide synthase-like domain. The AMP-binding domain exhibits in vitro enzymatic activity in activating long-chain fatty acids to form acyl-CoA. Expression pattern analysis shows that ''DWA1'' is preferentially expressed in vascular tissues and epidermal layers and strongly induced by drought stress. The ''dwa1'' mutant was highly sensitive to drought stress compared to the WT and DWA1-functional complementation （DWA1-FC）plants restored drought resistance. Further dissections uncovers that the ''dwa1'' mutant was impaired in cuticular wax accumulation and significantly suppressed many wax-related genes expression under drought conditions and reduced levels of very-long-chain fatty acids. Conversely, plants overexpressing ''DWA1'' were significantly up-regulated many wax-related genes expression under drought conditions and elevated levels of very-long-chain fatty acids. The results suggest that ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice&lt;ref name=&quot;ref1&quot;/&gt;.<br /> <br /> ===Mutation===<br /> [[File:dwa1.jpg|right|thumb|250px|Fig.1 Screening of mutants(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa2.jpg|right|thumb|250px|Fig.2 phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa3.jpg|right|thumb|250px|Fig.3 functional complementation assay analysis(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa4.jpg|right|thumb|250px|Fig.4 Overexpression of ''DWA1'' analysis(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> '''(1)''' '''Screening of mutants(Identification of the drought-hypersensitive ''dwa1'' mutant in rice.)'''&lt;br&gt;<br /> ''dwa1'' mutant is observed by inserting T-DNA in the sixth exon of ''DWA1''(Fig.1A and B).RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1C and D).&lt;br&gt;<br /> '''(2)''' '''phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT'''&lt;br&gt;<br /> During the course of drought treatment, the mutant wilted and exhibited leaf rolling earlier than the WT. After severe drought treatment followed by rewatering, more than 90% of the WT plants survive, whereas the ''dwa1'' mutant plants are almost dead(Fig.2A and C). Water loss rate of mutant is faster than WT(Fig.2B).Furthermore, SEM analysis indicate that the deposition of vertical plate-like wax crystals on the ''dwa1'' leaf cuticle is severely reduced under normal and drought conditions in contrast to WT leaf(Fig.2D). The contents of individual FA components is obvious difference between the ''dwa1'' mutant and WT. Among them, VLCFAs are significantly reduced, whereas the levels of are slightly higher in the ''dwa1'' mutant(Fig.2E). The gene expression levels of wax-related genes are obviously different under drought treatment(Fig.2F)&lt;br&gt;<br /> '''(3) functional complementation assay analysis'''&lt;br&gt;<br /> DWA1-functional complementation(DWA1-FC)lines which is inserted the full-length genomic fragment of ''DWA1'' into the ''dwa1'' mutant restore drought resistance(Fig.3).&lt;br&gt;<br /> '''(4)''' '''Overexpression of ''DWA1'' analysis'''&lt;br&gt;<br /> There are three independent overexpression plants(U7,U9 and U10) and a negative control (U4),and there is no visual differences in the epicuticular wax load compared with the control (Fig. 4B).But the VLCFA constituents increase in the overexpression lines compared with WT. However, the contents of LCFAs are significantly reduced in the overexpression plants. The alkanes and primary alcohols showed no significant changes (Fig. 4C). Under normal growth conditions, the expression levels of wax-related genes enhance in the overexpression plants (Fig.4D).<br /> <br /> ===Expression===<br /> [[File:图片4.png|right|thumb|250px|Fig.5 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:图片5.png|right|thumb|250px|Fig.6 Drought-induced expression of ''DWA1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> '''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.5A).&lt;br&gt;<br /> '''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.5B1 and 2), axillary bud (Fig.5B3), young panicle (Fig.5B4),spikelet (Fig.5B5), stem (Fig.5B6), young panicle (Fig.5B7), pistil (Fig.5B8), stamen (Fig.5B9), young embryo (Fig.5B10), SEM picture of rice leaf cuticle (Fig.5B11), cross-sections of stem (Fig.5B12) and leaf (Fig.5B13),and semithin sections of leaf (Fig.5B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.&lt;br&gt;<br /> '''(3)'''What's more,after various phytohormones treatment,''DWA1'' expresses higher,such as abscisic acid, jasmonic acid, indole-3-acetic acid, and<br /> gibberellic acid (Fig.5C).&lt;br&gt;<br /> '''(4)'''Under drought treatment,GUS staining shows that''DWA1'' is induced.For example,in Fig.6B,GUS staining in leaf before (1 and 3) and after (2 and 4) drought stress is shown.&lt;br&gt;<br /> <br /> ===Evolution===<br /> At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region&lt;ref name=&quot;ref1&quot;/&gt;(Fig.7).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi&lt;ref name=&quot;ref2&quot;/&gt;,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain&lt;ref name=&quot;ref1&quot;/&gt;.<br /> [[File:1(DWA1).png|center|thumb|500px|Fig.7 Unrooted phylogenetic tree and domain architecture of DWA1 protein orthologs in vascular plants and microbial relatives(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> <br /> ==Labs working on this gene==<br /> *National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan,China<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Xiaoyi Zhu, Lizhong Xiong.（2013）Putative megaenzyme DWA1 plays essential roles in drought resistance by regulating stress-induced wax<br /> deposition in rice.PANS 110(44): 17790-17795.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Marahiel MA, Stachelhaus T, Mootz HD.(1997) Modular peptide synthetases involved in nonribosomal peptide synthesis.Chem Rev 97(7):2651–2674.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os04g0473900|<br /> Description = Adrenodoxin reductase family protein|<br /> Version = NM_001187049.1 GI:297723228 GeneID:9266980|<br /> Length = 9882 bp|<br /> Definition = Oryza sativa Japonica Group Os04g0473900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|<br /> AP = Chromosome 4:24097480..24107361|<br /> CDS = 24097480..24100068,24100784..24104053,24104151..24104329,24104410..24104769,24104855..24105296&lt;br&gt;,24105409..24105542,24105689..24105892,24107127..24107361|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008397:24097480..24107361<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:24097480..24107361<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactgatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttctggctcagaaaggcatctcaattccagattcttctgatcaggttggacttgttgtgattgcagaggttagagagggtaaggcagtctctaaagaggttgttaacaacatcaaagcacgtgtggtggaggagcatggtgtagctgttgcatctgtgaagcttatcaagcctagaaccatatgcaagacaacatctgggaagatacgaagatttgaatgcatgaggcagtttgttgacaatactcttagtttggctaagggcaaccatgtatctaagaaaaagggtctttttcggtctctgacaacaggaactggcatggagtcaaagaggtccctcttgaggcaaactgttgacctcaccattagccattggcccaaaagccaagttaaaaactccaatgaaataactgagtttctgacacaaatagtgtcagagcacactggcatttcaaaggataaaatttctctaactgacagtctaccttcgtatggttttgattctattgcagtggtacgagcagcccagaagctctcagattttcttggtgttcctgttggggccattgacatctttacagcaagctgcatttcggagcttgcaagcttcttggagaatttggtgcataagtcacaaacacagttggcaccttggcccaaaagcaaagtgaaaaactccaaggtgattatcgagtttctgacaaagattgtatcagatcaaacagggattccaaaggataaaatttctccgatggatagcctaccttcatatggttttgattcaattgcggtggtccaagcagcacagaaactctccgattttctcggtgtacctgttggagccattgacatatttacagctggttgcatttctgagctagctaccttcttggagaatttggcgcataagtcacagtcacagttggcacctggtgcatcttgctatattgaggatgagactcaagtggatcccatggatgccattagtccagaattcagtgttttgggtacgggcatcctgcaactgctggctcttacttatgtctgttttgtgctgctactcccagcatatcttgctagttcgacatacatgagcattttctctacagtcagtctggtcagatcaccattgctgagctatcttagttctttggttatggcacctattgtgtggatcttttatatctcgctcacttctttatcactgtctatcttggggaagtccttcctacagccaaattatgttctgatccctgatgtttcaatatggtcagttgactttgtgaaatggtgggcactaaacaaagcacaagcattagctgcaaagatgctagctgtgcatctaaagggcacaattttcttaaattattggttcaagatgcaaggagctcggattggttcatctgttgtcatcgacacagtagacataaccgatccatccctgttgacggttgcagatggtgcagttcttgcagaaggcgctcttgttcagggccatgaagtctgtaatgaagtgctgagttttaggcctatctggattggctgtgaggcttcgattggtccttatgcggtgcttcagaagggaactgttgtggaagatggagctgttgttccaccattgcagaagactggggctgggaaatcaacacgtcggactagcagaacttctgtaagcataaagaaagaagcagccaaagcaaacatgatacttgaacacctcgttagcatctacgcagttggcatcctgggtgctctgtccggtgcaatcgtctacacactatacactcacttgtccggcaaagcggcttcgcctctgcatttctcctttgcatgcattgctggtgcgttccactggttaccagctgccatcactgcctatgctgttattgtccaggagacacccacaagtgcactgtcatttgctctcttcactgccttcgccgacctgagctatggtgtcatcctcagtattctcaccagtataaccagcagagcccttgctgcaaagcctggaaccaagcagaatggcattgcgagtttgatccaccggcgcataaccatctctgctcatgtcaggtttgccaagatgctatctggaacagaagccttctgcgtgtacctgaggcttctcggagcaaagattggcaggcattgctctatccgagccatcaatccggtggcgaatcctgagctgatcagcgtcggtgatggcgtccatctaggtgacttctgcaatattgttccagggttctactccaaggggggattcaccagtgcagagattaaggtccaggagaacaccgtggttggcagtggcagcttgctcctccctggctgtgtcctccaggagaatgtcatcctcggcgcgctctccgtcgcgccggagaacgctgttctccggcgtggcggcgtctacgtcgggtcacagtctccggccatggtgaagaacaccttgcttgacgaggatgaacggatcgaagagatggatcaggcatacaagaagattgttggcaacctcgcggcgaacttggccatcaccaccatgaacgtcaagtcgaggtacttccaccgcatcggcgtgagcggccgcggcgtcctgaggatgtacgaggagataccgtcgtttccccggcacaagatcttcgcctccggcaagtccttcccggtgatcgtccgccacagcaacagcctgagcgccgacgacgacgcgaggctcgacgcccgcggcgccgcggtgcggatcctctcggacaacgacggcgaggcgcctctcctcgacctcacactcaagagcggcaaggcgttctacgcgcgcaccatcgccgacttcgccacctggctggtctgcggcctgccggcgagggaggagcaggtcaagcgctcgccgcacatccgcgacgccgtctggggctctctccggagcaccgactcgtacaccgtgctccactactactccaacatatgccggctgctgcggttcgacgacggcagggagatgtacgccaagttcaagctccgtcctgctgatccagacgtccccgaggactccggcaaggtggtgccgaggggcatcctgccgccggagaccggcgcgatccccagggacgaggatgacacgcgccctctgctcttcctcgccgacgacttccgccggagagtgggctcaccggacggggtgcgctacgtgttccagctgcagctcagggaggtgcccaccgacgccgccgcccgcgacgtcgccctcgactgcacccggccgtgggacgaggccgagttcccttacatcgacgtcggcgaggtcagcatcggccgcaacctcccgacggaggagacggagaagctcgagttcaacccgttcctccggtgccccgaggtggacgtcatcccggcgacgtcgtgcgcgcagagcgcgtccatcgaccacggccgctcgctggtctacgagatatgccagcgcctgcggaacggcgagccgctgcccgcgtcgtggcgagctttcctggagcagtcggacaccaagatcgacctgtcaggatgcccggtcgctgccgccgcgacacccacaaggtctaacgccggcgacgccacgaaggtgacgctggccaggacgtggtaccaggcgctctgggccacgctgtgccagccgctgctgcaaacgctggtgccgtactctgtcctgggcctcgtcatcttcctcccgctcaggggcctcctcgccgtcgccgcggccacccgcttcccgctctactggctcctcccggcgttctgggccgcgtcgggcgtcgccgccatggccacgtgcgcggcggcgaagtgggcgctggtcggcagcagggtcgacggcgacacggtgcacatctggtcgccggccgtgttcctggacacggtctggcaggccgtgcgcgccgccacggcggagtacttcgccgagctgacgcccgggtcggcgccgttcgcggcgtggatgcgggtgatgggcgcctccgtgtcccccggcgacggcgtgtacgtggactccatgggcgcgctgctgaacccggagatggtgcgcctggagcgcggcgccgccgtcgggcgggacgcgctgctgttcggccacgtgtacgagggcgaggccgggaaggtgaagttcggcgccgtgtccgtcggggaggacgggttcgtcggcagccgcgccgtggccatgcccagcgtcacggtggacgacggcggctgcctcgccgcgctcgggctggccatgaagggggagacggtgaagcacagcatgtaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MEERRATSPSRHSFCRDALGRRGGESGGGLVYIYRSPLRSPLQR HGLNREWRLERWRWRWRWEAATWRQRAAAHARIGEMDPKRSIDDQFSKLHPSLPVDTR IGIVGAGPSGLSAAYALAKLGYRNVTLFEKCHTVSGMCESIDIEEELGSDFEEMDTHK LSLIDSQTGNIRDLEVAEDYVSMVSLTLKLQDEANKSGRAGLHALSGLASDPTHEFLK QNGINSMPKSVAYGYTASGYGFVQDMPYAFIQEFTRTSMAGKIRRFKHGYMSMWERLS KSLPFEVFCDTQVLNVKRNSCGANVTIKNNNGEKQVLEFDKIILSGAVAFKNSKTYRS SSLTDGESEVVELNNLERELFSKVQTIDYYTTVVKINGFEHIPKGFYYFGEFMEDPTT IGHPVAMQRFFADTNIFLFWSYGNSADIKGSFVAKCVTDVVSSMGGNVQKVILQRRFK YFPHVSSKDMKDGFYERLESQLQGFQNTYYVGGLLAFELTERNASYSIMAVCKHFAID GEGELTPYVKRLFPLSHNRNPSPPRDLGELEGVEFPDLPSLDGYLQYWGTHKVTEKNV IYTWINEEGKLMNRRTYQELHGNASYIAQKLLTSTKPVIKPGDRVLLIHLPGLEFIDA FFGCIRAGVIPVPVLPPDPMQSGGQALLKVENISKMCNAVAILSTSSYHAAVRAGYIK NIVTLAKRVQKCSAQWPDIPWIHTDSWIKNYRRSSDSFNSDTVLFTKPQPSDLCFLQF TSGSTGDAKGVMITHEGLIHNVKTMKKRYRSTSKTVLVSWLPQYHDMGLIGGLFTALV SGGTSVLFSPMIFIRNPLLWLQTINDYHGTHSAGPNFAFELVIRRLEAEKNKVYDLSS MVFLMIAAEPVRQKTVRRFIELTQPFGLSEGVLAPGYGLAENCVYVTCAFGECKPVFI DWQGRVCCGYVEQDDTDTLIRIVDPDSLTEHQEDGVEGEIWISSPSSGVGYWGNSEMS QRTFFNQLKNHPNKKFTRTGDLGRTIDGNLFITGRIKDLIIVAGRNIYSADVEKTVES SSEVLRPGCCAVVGIPEEVLAQKGISIPDSSDQVGLVVIAEVREGKAVSKEVVNNIKA RVVEEHGVAVASVKLIKPRTICKTTSGKIRRFECMRQFVDNTLSLAKGNHVSKKKGLF RSLTTGTGMESKRSLLRQTVDLTISHWPKSQVKNSNEITEFLTQIVSEHTGISKDKIS LTDSLPSYGFDSIAVVRAAQKLSDFLGVPVGAIDIFTASCISELASFLENLVHKSQTQ LAPWPKSKVKNSKVIIEFLTKIVSDQTGIPKDKISPMDSLPSYGFDSIAVVQAAQKLS DFLGVPVGAIDIFTAGCISELATFLENLAHKSQSQLAPGASCYIEDETQVDPMDAISP EFSVLGTGILQLLALTYVCFVLLLPAYLASSTYMSIFSTVSLVRSPLLSYLSSLVMAP IVWIFYISLTSLSLSILGKSFLQPNYVLIPDVSIWSVDFVKWWALNKAQALAAKMLAV HLKGTIFLNYWFKMQGARIGSSVVIDTVDITDPSLLTVADGAVLAEGALVQGHEVCNE VLSFRPIWIGCEASIGPYAVLQKGTVVEDGAVVPPLQKTGAGKSTRRTSRTSVSIKKE AAKANMILEHLVSIYAVGILGALSGAIVYTLYTHLSGKAASPLHFSFACIAGAFHWLP AAITAYAVIVQETPTSALSFALFTAFADLSYGVILSILTSITSRALAAKPGTKQNGIA SLIHRRITISAHVRFAKMLSGTEAFCVYLRLLGAKIGRHCSIRAINPVANPELISVGD GVHLGDFCNIVPGFYSKGGFTSAEIKVQENTVVGSGSLLLPGCVLQENVILGALSVAP ENAVLRRGGVYVGSQSPAMVKNTLLDEDERIEEMDQAYKKIVGNLAANLAITTMNVKS RYFHRIGVSGRGVLRMYEEIPSFPRHKIFASGKSFPVIVRHSNSLSADDDARLDARGA AVRILSDNDGEAPLLDLTLKSGKAFYARTIADFATWLVCGLPAREEQVKRSPHIRDAV WGSLRSTDSYTVLHYYSNICRLLRFDDGREMYAKFKLRPADPDVPEDSGKVVPRGILP PETGAIPRDEDDTRPLLFLADDFRRRVGSPDGVRYVFQLQLREVPTDAAARDVALDCT RPWDEAEFPYIDVGEVSIGRNLPTEETEKLEFNPFLRCPEVDVIPATSCAQSASIDHG RSLVYEICQRLRNGEPLPASWRAFLEQSDTKIDLSGCPVAAAATPTRSNAGDATKVTL ARTWYQALWATLCQPLLQTLVPYSVLGLVIFLPLRGLLAVAAATRFPLYWLLPAFWAA SGVAAMATCAAAKWALVGSRVDGDTVHIWSPAVFLDTVWQAVRAATAEYFAELTPGSA PFAAWMRVMGASVSPGDGVYVDSMGALLNPEMVRLERGAAVGRDALLFGHVYEGEAGK VKFGAVSVGEDGFVGSRAVAMPSVTVDDGGCLAALGLAMKGETVKHSM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;7294..9882#3309..6578#3033..3211#2593..2952#2066..2507#1820..1953#1470..1673#1..235#atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggaggtatggattccccttccccactcgctgcgtgatgaaccgatgaaccgtagtacttgatgatatgcctgatttgcttcggcattgtgccgcttctctgttagctgggagtttgtggcgtgtcgtttaactgaagtagagatgtgatgatgatctgaggtgagcgccattttttttttttggctaactctgtttgttctattcagtgaatatgagctctcttttttgtcccctttttatcattctgcttctatgggtcgcggagcgttcttggttggtgcagtgcaactgcaatgtcagtatgcatgccatagtaatgtgatggtgggatgtaatgctcctctgcttgctcgagctctttcttggttgtctatctatcttggatgatttgatcaatgtactacttattatctgctgatagtacccggggtccatttgcgctagtattgaacttcttcttctcttataatttgctgaaatcgcgagattatcgcttaggtttatcaatcagaacattatcatgtttatgcttcggttcgttcggtactggcacatgtcaaataagcggaaattctgctactttttcggcttaccatgaatggcacccaacatagcgtggttgttttgtcatgttgaactagtacaaatttttgcaccttaatatttgctttccgtgcatatccaattaacctgcccatcaaacttcttttcagatagctattattggggaaaaatattttcccagcttcttttatagtgactagactgtttatacatgcctgcgtaattgcttccttttgatgagctagtacattatagaccttgattttgttttgatggtacgggttggttattggtgtccatataatgaaattgcaacttgcgaccactccatgatagttacctggtcattccttgttggttaataagctattatttcaaatatgaatgttcatcctacttgtcatattatagacatttgtcacaattcaagtgaggaagaaattctgtaattttctatgggctagttgctgcgttaactacaataatggggctgacatcctgaggttatagtgttcgattaccaagttctaatttattaagaggaatgcaagtcaacatgcatgcctagcccatttacttttaatgagtagtgtaacttctgttgcagaagtactaatgacagctttctatataaatttcacaggttgatttatcaaggacaacaattcgtcagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaaggtgaggaaactactcagcaaaattatacagtcatcattttgtgcacacttttagttatcagcaacattcgtatgtaggacgaatatatgatttgggaggacaagttatagcagcaaacagtgcccctgttataactcaccttgcagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggtgagttgttctagaataaaatttaaatttgaggtaactggataataagcatctaattatttataatattctctctatttcttgcatgcgtgttattcaataattcttacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactggtgagcatattttaaattcagtttggctggcattgaagctatagtatttcagttgccttattagtatgtatgatcttttgttcagatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaaggtatatatgttaataatatttctcctgttgcaaggtcttcttttttctcgtgataacattggtaatttcctgcattgcagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaaggtgagcaagaaataatggcgtaatatatctcaattattaattccaccttcatcaaatttcttagagaaattgtgcatatatttttccatacttgcagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagagg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ica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001187049.1 RefSeq:Os04g0473900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 4]]<br /> [[Category:Chromosome 4]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os04g0473900&diff=175259 2014-06-01T01:56:19Z

<p>Mayamei0424: /* Expression */</p> <hr /> <div>''DWA1'' encodes an enzymatic megaprotein conserved in vascular plants，and ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''DWA1'' encodes an enzymatic megaprotein (2,391 aa in length) conserved in vascular plants, including an oxidoreductase-like domain, a prokaryotic nonribosomal peptide synthetase-like module, an AMP-binding domain, and an allene oxide synthase-like domain. The AMP-binding domain exhibits in vitro enzymatic activity in activating long-chain fatty acids to form acyl-CoA. Expression pattern analysis shows that ''DWA1'' is preferentially expressed in vascular tissues and epidermal layers and strongly induced by drought stress. The ''dwa1'' mutant was highly sensitive to drought stress compared to the WT and DWA1-functional complementation （DWA1-FC）plants restored drought resistance. Further dissections uncovers that the ''dwa1'' mutant was impaired in cuticular wax accumulation and significantly suppressed many wax-related genes expression under drought conditions and reduced levels of very-long-chain fatty acids. Conversely, plants overexpressing ''DWA1'' were significantly up-regulated many wax-related genes expression under drought conditions and elevated levels of very-long-chain fatty acids. The results suggest that ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice&lt;ref name=&quot;ref1&quot;/&gt;.<br /> <br /> ===Mutation===<br /> [[File:dwa1.jpg|right|thumb|200px|Fig.1 Screening of mutants(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa2.jpg|right|thumb|200px|Fig.2 phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa3.jpg|right|thumb|200px|Fig.3 functional complementation assay analysis(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa4.jpg|right|thumb|200px|Fig.4 Overexpression of ''DWA1'' analysis(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> '''(1)''' '''Screening of mutants(Identification of the drought-hypersensitive ''dwa1'' mutant in rice.)'''&lt;br&gt;<br /> ''dwa1'' mutant is observed by inserting T-DNA in the sixth exon of ''DWA1''(Fig.1A and B).RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1C and D).&lt;br&gt;<br /> '''(2)''' '''phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT'''&lt;br&gt;<br /> During the course of drought treatment, the mutant wilted and exhibited leaf rolling earlier than the WT. After severe drought treatment followed by rewatering, more than 90% of the WT plants survive, whereas the ''dwa1'' mutant plants are almost dead(Fig.2A and C). Water loss rate of mutant is faster than WT(Fig.2B).Furthermore, SEM analysis indicate that the deposition of vertical plate-like wax crystals on the ''dwa1'' leaf cuticle is severely reduced under normal and drought conditions in contrast to WT leaf(Fig.2D). The contents of individual FA components is obvious difference between the ''dwa1'' mutant and WT. Among them, VLCFAs are significantly reduced, whereas the levels of are slightly higher in the ''dwa1'' mutant(Fig.2E). The gene expression levels of wax-related genes are obviously different under drought treatment(Fig.2F)&lt;br&gt;<br /> '''(3) functional complementation assay analysis'''&lt;br&gt;<br /> DWA1-functional complementation(DWA1-FC)lines which is inserted the full-length genomic fragment of ''DWA1'' into the ''dwa1'' mutant restore drought resistance(Fig.3).&lt;br&gt;<br /> '''(4)''' '''Overexpression of ''DWA1'' analysis'''&lt;br&gt;<br /> There are three independent overexpression plants(U7,U9 and U10) and a negative control (U4),and there is no visual differences in the epicuticular wax load compared with the control (Fig. 4B).But the VLCFA constituents increase in the overexpression lines compared with WT. However, the contents of LCFAs are significantly reduced in the overexpression plants. The alkanes and primary alcohols showed no significant changes (Fig. 4C). Under normal growth conditions, the expression levels of wax-related genes enhance in the overexpression plants (Fig.4D).<br /> <br /> ===Expression===<br /> [[File:图片4.png|right|thumb|250px|Fig.5 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:图片5.png|right|thumb|250px|Fig.6 Drought-induced expression of ''DWA1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> '''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.5A).&lt;br&gt;<br /> '''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.5B1 and 2), axillary bud (Fig.5B3), young panicle (Fig.5B4),spikelet (Fig.5B5), stem (Fig.5B6), young panicle (Fig.5B7), pistil (Fig.5B8), stamen (Fig.5B9), young embryo (Fig.5B10), SEM picture of rice leaf cuticle (Fig.5B11), cross-sections of stem (Fig.5B12) and leaf (Fig.5B13),and semithin sections of leaf (Fig.5B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.&lt;br&gt;<br /> '''(3)'''What's more,after various phytohormones treatment,''DWA1'' expresses higher,such as abscisic acid, jasmonic acid, indole-3-acetic acid, and<br /> gibberellic acid (Fig.5C).&lt;br&gt;<br /> '''(4)'''Under drought treatment,GUS staining shows that''DWA1'' is induced.For example,in Fig.6B,GUS staining in leaf before (1 and 3) and after (2 and 4) drought stress is shown.&lt;br&gt;<br /> <br /> ===Evolution===<br /> At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region&lt;ref name=&quot;ref1&quot;/&gt;(Fig.7).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi&lt;ref name=&quot;ref2&quot;/&gt;,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain&lt;ref name=&quot;ref1&quot;/&gt;.<br /> [[File:1(DWA1).png|center|thumb|500px|Fig.7 Unrooted phylogenetic tree and domain architecture of DWA1 protein orthologs in vascular plants and microbial relatives(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> <br /> ==Labs working on this gene==<br /> *National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan,China<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Xiaoyi Zhu, Lizhong Xiong.（2013）Putative megaenzyme DWA1 plays essential roles in drought resistance by regulating stress-induced wax<br /> deposition in rice.PANS 110(44): 17790-17795.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Marahiel MA, Stachelhaus T, Mootz HD.(1997) Modular peptide synthetases involved in nonribosomal peptide synthesis.Chem Rev 97(7):2651–2674.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os04g0473900|<br /> Description = Adrenodoxin reductase family protein|<br /> Version = NM_001187049.1 GI:297723228 GeneID:9266980|<br /> Length = 9882 bp|<br /> Definition = Oryza sativa Japonica Group Os04g0473900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|<br /> AP = Chromosome 4:24097480..24107361|<br /> CDS = 24097480..24100068,24100784..24104053,24104151..24104329,24104410..24104769,24104855..24105296&lt;br&gt;,24105409..24105542,24105689..24105892,24107127..24107361|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008397:24097480..24107361<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:24097480..24107361<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactgatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttctggctcagaaaggcatctcaattccagattcttctgatcaggttggacttgttgtgattgcagaggttagagagggtaaggcagtctctaaagaggttgttaacaacatcaaagcacgtgtggtggaggagcatggtgtagctgttgcatctgtgaagcttatcaagcctagaaccatatgcaagacaacatctgggaagatacgaagatttgaatgcatgaggcagtttgttgacaatactcttagtttggctaagggcaaccatgtatctaagaaaaagggtctttttcggtctctgacaacaggaactggcatggagtcaaagaggtccctcttgaggcaaactgttgacctcaccattagccattggcccaaaagccaagttaaaaactccaatgaaataactgagtttctgacacaaatagtgtcagagcacactggcatttcaaaggataaaatttctctaactgacagtctaccttcgtatggttttgattctattgcagtggtacgagcagcccagaagctctcagattttcttggtgttcctgttggggccattgacatctttacagcaagctgcatttcggagcttgcaagcttcttggagaatttggtgcataagtcacaaacacagttggcaccttggcccaaaagcaaagtgaaaaactccaaggtgattatcgagtttctgacaaagattgtatcagatcaaacagggattccaaaggataaaatttctccgatggatagcctaccttcatatggttttgattcaattgcggtggtccaagcagcacagaaactctccgattttctcggtgtacctgttggagccattgacatatttacagctggttgcatttctgagctagctaccttcttggagaatttggcgcataagtcacagtcacagttggcacctggtgcatcttgctatattgaggatgagactcaagtggatcccatggatgccattagtccagaattcagtgttttgggtacgggcatcctgcaactgctggctcttacttatgtctgttttgtgctgctactcccagcatatcttgctagttcgacatacatgagcattttctctacagtcagtctggtcagatcaccattgctgagctatcttagttctttggttatggcacctattgtgtggatcttttatatctcgctcacttctttatcactgtctatcttggggaagtccttcctacagccaaattatgttctgatccctgatgtttcaatatggtcagttgactttgtgaaatggtgggcactaaacaaagcacaagcattagctgcaaagatgctagctgtgcatctaaagggcacaattttcttaaattattggttcaagatgcaaggagctcggattggttcatctgttgtcatcgacacagtagacataaccgatccatccctgttgacggttgcagatggtgcagttcttgcagaaggcgctcttgttcagggccatgaagtctgtaatgaagtgctgagttttaggcctatctggattggctgtgaggcttcgattggtccttatgcggtgcttcagaagggaactgttgtggaagatggagctgttgttccaccattgcagaagactggggctgggaaatcaacacgtcggactagcagaacttctgtaagcataaagaaagaagcagccaaagcaaacatgatacttgaacacctcgttagcatctacgcagttggcatcctgggtgctctgtccggtgcaatcgtctacacactatacactcacttgtccggcaaagcggcttcgcctctgcatttctcctttgcatgcattgctggtgcgttccactggttaccagctgccatcactgcctatgctgttattgtccaggagacacccacaagtgcactgtcatttgctctcttcactgccttcgccgacctgagctatggtgtcatcctcagtattctcaccagtataaccagcagagcccttgctgcaaagcctggaaccaagcagaatggcattgcgagtttgatccaccggcgcataaccatctctgctcatgtcaggtttgccaagatgctatctggaacagaagccttctgcgtgtacctgaggcttctcggagcaaagattggcaggcattgctctatccgagccatcaatccggtggcgaatcctgagctgatcagcgtcggtgatggcgtccatctaggtgacttctgcaatattgttccagggttctactccaaggggggattcaccagtgcagagattaaggtccaggagaacaccgtggttggcagtggcagcttgctcctccctggctgtgtcctccaggagaatgtcatcctcggcgcgctctccgtcgcgccggagaacgctgttctccggcgtggcggcgtctacgtcgggtcacagtctccggccatggtgaagaacaccttgcttgacgaggatgaacggatcgaagagatggatcaggcatacaagaagattgttggcaacctcgcggcgaacttggccatcaccaccatgaacgtcaagtcgaggtacttccaccgcatcggcgtgagcggccgcggcgtcctgaggatgtacgaggagataccgtcgtttccccggcacaagatcttcgcctccggcaagtccttcccggtgatcgtccgccacagcaacagcctgagcgccgacgacgacgcgaggctcgacgcccgcggcgccgcggtgcggatcctctcggacaacgacggcgaggcgcctctcctcgacctcacactcaagagcggcaaggcgttctacgcgcgcaccatcgccgacttcgccacctggctggtctgcggcctgccggcgagggaggagcaggtcaagcgctcgccgcacatccgcgacgccgtctggggctctctccggagcaccgactcgtacaccgtgctccactactactccaacatatgccggctgctgcggttcgacgacggcagggagatgtacgccaagttcaagctccgtcctgctgatccagacgtccccgaggactccggcaaggtggtgccgaggggcatcctgccgccggagaccggcgcgatccccagggacgaggatgacacgcgccctctgctcttcctcgccgacgacttccgccggagagtgggctcaccggacggggtgcgctacgtgttccagctgcagctcagggaggtgcccaccgacgccgccgcccgcgacgtcgccctcgactgcacccggccgtgggacgaggccgagttcccttacatcgacgtcggcgaggtcagcatcggccgcaacctcccgacggaggagacggagaagctcgagttcaacccgttcctccggtgccccgaggtggacgtcatcccggcgacgtcgtgcgcgcagagcgcgtccatcgaccacggccgctcgctggtctacgagatatgccagcgcctgcggaacggcgagccgctgcccgcgtcgtggcgagctttcctggagcagtcggacaccaagatcgacctgtcaggatgcccggtcgctgccgccgcgacacccacaaggtctaacgccggcgacgccacgaaggtgacgctggccaggacgtggtaccaggcgctctgggccacgctgtgccagccgctgctgcaaacgctggtgccgtactctgtcctgggcctcgtcatcttcctcccgctcaggggcctcctcgccgtcgccgcggccacccgcttcccgctctactggctcctcccggcgttctgggccgcgtcgggcgtcgccgccatggccacgtgcgcggcggcgaagtgggcgctggtcggcagcagggtcgacggcgacacggtgcacatctggtcgccggccgtgttcctggacacggtctggcaggccgtgcgcgccgccacggcggagtacttcgccgagctgacgcccgggtcggcgccgttcgcggcgtggatgcgggtgatgggcgcctccgtgtcccccggcgacggcgtgtacgtggactccatgggcgcgctgctgaacccggagatggtgcgcctggagcgcggcgccgccgtcgggcgggacgcgctgctgttcggccacgtgtacgagggcgaggccgggaaggtgaagttcggcgccgtgtccgtcggggaggacgggttcgtcggcagccgcgccgtggccatgcccagcgtcacggtggacgacggcggctgcctcgccgcgctcgggctggccatgaagggggagacggtgaagcacagcatgtaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MEERRATSPSRHSFCRDALGRRGGESGGGLVYIYRSPLRSPLQR HGLNREWRLERWRWRWRWEAATWRQRAAAHARIGEMDPKRSIDDQFSKLHPSLPVDTR IGIVGAGPSGLSAAYALAKLGYRNVTLFEKCHTVSGMCESIDIEEELGSDFEEMDTHK LSLIDSQTGNIRDLEVAEDYVSMVSLTLKLQDEANKSGRAGLHALSGLASDPTHEFLK QNGINSMPKSVAYGYTASGYGFVQDMPYAFIQEFTRTSMAGKIRRFKHGYMSMWERLS KSLPFEVFCDTQVLNVKRNSCGANVTIKNNNGEKQVLEFDKIILSGAVAFKNSKTYRS SSLTDGESEVVELNNLERELFSKVQTIDYYTTVVKINGFEHIPKGFYYFGEFMEDPTT IGHPVAMQRFFADTNIFLFWSYGNSADIKGSFVAKCVTDVVSSMGGNVQKVILQRRFK YFPHVSSKDMKDGFYERLESQLQGFQNTYYVGGLLAFELTERNASYSIMAVCKHFAID GEGELTPYVKRLFPLSHNRNPSPPRDLGELEGVEFPDLPSLDGYLQYWGTHKVTEKNV IYTWINEEGKLMNRRTYQELHGNASYIAQKLLTSTKPVIKPGDRVLLIHLPGLEFIDA FFGCIRAGVIPVPVLPPDPMQSGGQALLKVENISKMCNAVAILSTSSYHAAVRAGYIK NIVTLAKRVQKCSAQWPDIPWIHTDSWIKNYRRSSDSFNSDTVLFTKPQPSDLCFLQF TSGSTGDAKGVMITHEGLIHNVKTMKKRYRSTSKTVLVSWLPQYHDMGLIGGLFTALV SGGTSVLFSPMIFIRNPLLWLQTINDYHGTHSAGPNFAFELVIRRLEAEKNKVYDLSS MVFLMIAAEPVRQKTVRRFIELTQPFGLSEGVLAPGYGLAENCVYVTCAFGECKPVFI DWQGRVCCGYVEQDDTDTLIRIVDPDSLTEHQEDGVEGEIWISSPSSGVGYWGNSEMS QRTFFNQLKNHPNKKFTRTGDLGRTIDGNLFITGRIKDLIIVAGRNIYSADVEKTVES SSEVLRPGCCAVVGIPEEVLAQKGISIPDSSDQVGLVVIAEVREGKAVSKEVVNNIKA RVVEEHGVAVASVKLIKPRTICKTTSGKIRRFECMRQFVDNTLSLAKGNHVSKKKGLF RSLTTGTGMESKRSLLRQTVDLTISHWPKSQVKNSNEITEFLTQIVSEHTGISKDKIS LTDSLPSYGFDSIAVVRAAQKLSDFLGVPVGAIDIFTASCISELASFLENLVHKSQTQ LAPWPKSKVKNSKVIIEFLTKIVSDQTGIPKDKISPMDSLPSYGFDSIAVVQAAQKLS DFLGVPVGAIDIFTAGCISELATFLENLAHKSQSQLAPGASCYIEDETQVDPMDAISP EFSVLGTGILQLLALTYVCFVLLLPAYLASSTYMSIFSTVSLVRSPLLSYLSSLVMAP IVWIFYISLTSLSLSILGKSFLQPNYVLIPDVSIWSVDFVKWWALNKAQALAAKMLAV HLKGTIFLNYWFKMQGARIGSSVVIDTVDITDPSLLTVADGAVLAEGALVQGHEVCNE VLSFRPIWIGCEASIGPYAVLQKGTVVEDGAVVPPLQKTGAGKSTRRTSRTSVSIKKE AAKANMILEHLVSIYAVGILGALSGAIVYTLYTHLSGKAASPLHFSFACIAGAFHWLP AAITAYAVIVQETPTSALSFALFTAFADLSYGVILSILTSITSRALAAKPGTKQNGIA SLIHRRITISAHVRFAKMLSGTEAFCVYLRLLGAKIGRHCSIRAINPVANPELISVGD GVHLGDFCNIVPGFYSKGGFTSAEIKVQENTVVGSGSLLLPGCVLQENVILGALSVAP ENAVLRRGGVYVGSQSPAMVKNTLLDEDERIEEMDQAYKKIVGNLAANLAITTMNVKS RYFHRIGVSGRGVLRMYEEIPSFPRHKIFASGKSFPVIVRHSNSLSADDDARLDARGA AVRILSDNDGEAPLLDLTLKSGKAFYARTIADFATWLVCGLPAREEQVKRSPHIRDAV WGSLRSTDSYTVLHYYSNICRLLRFDDGREMYAKFKLRPADPDVPEDSGKVVPRGILP PETGAIPRDEDDTRPLLFLADDFRRRVGSPDGVRYVFQLQLREVPTDAAARDVALDCT RPWDEAEFPYIDVGEVSIGRNLPTEETEKLEFNPFLRCPEVDVIPATSCAQSASIDHG RSLVYEICQRLRNGEPLPASWRAFLEQSDTKIDLSGCPVAAAATPTRSNAGDATKVTL ARTWYQALWATLCQPLLQTLVPYSVLGLVIFLPLRGLLAVAAATRFPLYWLLPAFWAA SGVAAMATCAAAKWALVGSRVDGDTVHIWSPAVFLDTVWQAVRAATAEYFAELTPGSA PFAAWMRVMGASVSPGDGVYVDSMGALLNPEMVRLERGAAVGRDALLFGHVYEGEAGK VKFGAVSVGEDGFVGSRAVAMPSVTVDDGGCLAALGLAMKGETVKHSM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;7294..9882#3309..6578#3033..3211#2593..2952#2066..2507#1820..1953#1470..1673#1..235#atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggaggtatggattccccttccccactcgctgcgtgatgaaccgatgaaccgtagtacttgatgatatgcctgatttgcttcggcattgtgccgcttctctgttagctgggagtttgtggcgtgtcgtttaactgaagtagagatgtgatgatgatctgaggtgagcgccattttttttttttggctaactctgtttgttctattcagtgaatatgagctctcttttttgtcccctttttatcattctgcttctatgggtcgcggagcgttcttggttggtgcagtgcaactgcaatgtcagtatgcatgccatagtaatgtgatggtgggatgtaatgctcctctgcttgctcgagctctttcttggttgtctatctatcttggatgatttgatcaatgtactacttattatctgctgatagtacccggggtccatttgcgctagtattgaacttcttcttctcttataatttgctgaaatcgcgagattatcgcttaggtttatcaatcagaacattatcatgtttatgcttcggttcgttcggtactggcacatgtcaaataagcggaaattctgctactttttcggcttaccatgaatggcacccaacatagcgtggttgttttgtcatgttgaactagtacaaatttttgcaccttaatatttgctttccgtgcatatccaattaacctgcccatcaaacttcttttcagatagctattattggggaaaaatattttcccagcttcttttatagtgactagactgtttatacatgcctgcgtaattgcttccttttgatgagctagtacattatagaccttgattttgttttgatggtacgggttggttattggtgtccatataatgaaattgcaacttgcgaccactccatgatagttacctggtcattccttgttggttaataagctattatttcaaatatgaatgttcatcctacttgtcatattatagacatttgtcacaattcaagtgaggaagaaattctgtaattttctatgggctagttgctgcgttaactacaataatggggctgacatcctgaggttatagtgttcgattaccaagttctaatttattaagaggaatgcaagtcaacatgcatgcctagcccatttacttttaatgagtagtgtaacttctgttgcagaagtactaatgacagctttctatataaatttcacaggttgatttatcaaggacaacaattcgtcagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaaggtgaggaaactactcagcaaaattatacagtcatcattttgtgcacacttttagttatcagcaacattcgtatgtaggacgaatatatgatttgggaggacaagttatagcagcaaacagtgcccctgttataactcaccttgcagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggtgagttgttctagaataaaatttaaatttgaggtaactggataataagcatctaattatttataatattctctctatttcttgcatgcgtgttattcaataattcttacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactggtgagcatattttaaattcagtttggctggcattgaagctatagtatttcagttgccttattagtatgtatgatcttttgttcagatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaaggtatatatgttaataatatttctcctgttgcaaggtcttcttttttctcgtgataacattggtaatttcctgcattgcagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaaggtgagcaagaaataatggcgtaatatatctcaattattaattccaccttcatcaaatttcttagagaaattgtgcatatatttttccatacttgcagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagagg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ica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001187049.1 RefSeq:Os04g0473900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 4]]<br /> [[Category:Chromosome 4]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os04g0473900&diff=175257 2014-06-01T01:51:53Z

<p>Mayamei0424: /* Mutation */</p> <hr /> <div>''DWA1'' encodes an enzymatic megaprotein conserved in vascular plants，and ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''DWA1'' encodes an enzymatic megaprotein (2,391 aa in length) conserved in vascular plants, including an oxidoreductase-like domain, a prokaryotic nonribosomal peptide synthetase-like module, an AMP-binding domain, and an allene oxide synthase-like domain. The AMP-binding domain exhibits in vitro enzymatic activity in activating long-chain fatty acids to form acyl-CoA. Expression pattern analysis shows that ''DWA1'' is preferentially expressed in vascular tissues and epidermal layers and strongly induced by drought stress. The ''dwa1'' mutant was highly sensitive to drought stress compared to the WT and DWA1-functional complementation （DWA1-FC）plants restored drought resistance. Further dissections uncovers that the ''dwa1'' mutant was impaired in cuticular wax accumulation and significantly suppressed many wax-related genes expression under drought conditions and reduced levels of very-long-chain fatty acids. Conversely, plants overexpressing ''DWA1'' were significantly up-regulated many wax-related genes expression under drought conditions and elevated levels of very-long-chain fatty acids. The results suggest that ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice&lt;ref name=&quot;ref1&quot;/&gt;.<br /> <br /> ===Mutation===<br /> [[File:dwa1.jpg|right|thumb|200px|Fig.1 Screening of mutants(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa2.jpg|right|thumb|200px|Fig.2 phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa3.jpg|right|thumb|200px|Fig.3 functional complementation assay analysis(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa4.jpg|right|thumb|200px|Fig.4 Overexpression of ''DWA1'' analysis(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> '''(1)''' '''Screening of mutants(Identification of the drought-hypersensitive ''dwa1'' mutant in rice.)'''&lt;br&gt;<br /> ''dwa1'' mutant is observed by inserting T-DNA in the sixth exon of ''DWA1''(Fig.1A and B).RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1C and D).&lt;br&gt;<br /> '''(2)''' '''phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT'''&lt;br&gt;<br /> During the course of drought treatment, the mutant wilted and exhibited leaf rolling earlier than the WT. After severe drought treatment followed by rewatering, more than 90% of the WT plants survive, whereas the ''dwa1'' mutant plants are almost dead(Fig.2A and C). Water loss rate of mutant is faster than WT(Fig.2B).Furthermore, SEM analysis indicate that the deposition of vertical plate-like wax crystals on the ''dwa1'' leaf cuticle is severely reduced under normal and drought conditions in contrast to WT leaf(Fig.2D). The contents of individual FA components is obvious difference between the ''dwa1'' mutant and WT. Among them, VLCFAs are significantly reduced, whereas the levels of are slightly higher in the ''dwa1'' mutant(Fig.2E). The gene expression levels of wax-related genes are obviously different under drought treatment(Fig.2F)&lt;br&gt;<br /> '''(3) functional complementation assay analysis'''&lt;br&gt;<br /> DWA1-functional complementation(DWA1-FC)lines which is inserted the full-length genomic fragment of ''DWA1'' into the ''dwa1'' mutant restore drought resistance(Fig.3).&lt;br&gt;<br /> '''(4)''' '''Overexpression of ''DWA1'' analysis'''&lt;br&gt;<br /> There are three independent overexpression plants(U7,U9 and U10) and a negative control (U4),and there is no visual differences in the epicuticular wax load compared with the control (Fig. 4B).But the VLCFA constituents increase in the overexpression lines compared with WT. However, the contents of LCFAs are significantly reduced in the overexpression plants. The alkanes and primary alcohols showed no significant changes (Fig. 4C). Under normal growth conditions, the expression levels of wax-related genes enhance in the overexpression plants (Fig.4D).<br /> <br /> ===Expression===<br /> [[File:图片4.png|right|thumb|200px|Fig.5 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:图片5.png|right|thumb|200px|Fig.6 Drought-induced expression of ''DWA1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> '''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.5A).&lt;br&gt;<br /> '''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.5B1 and 2), axillary bud (Fig.5B3), young panicle (Fig.5B4),spikelet (Fig.5B5), stem (Fig.5B6), young panicle (Fig.5B7), pistil (Fig.5B8), stamen (Fig.5B9), young embryo (Fig.5B10), SEM picture of rice leaf cuticle (Fig.5B11), cross-sections of stem (Fig.5B12) and leaf (Fig.5B13),and semithin sections of leaf (Fig.5B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.&lt;br&gt;<br /> '''(3)'''What's more,after various phytohormones treatment,''DWA1'' expresses higher,such as abscisic acid, jasmonic acid, indole-3-acetic acid, and<br /> gibberellic acid (Fig.5C).&lt;br&gt;<br /> '''(4)'''Under drought treatment,GUS staining shows that''DWA1'' is induced.For example,in Fig.6B,GUS staining in leaf before (1 and 3) and after (2 and 4) drought stress is shown.&lt;br&gt;<br /> <br /> ===Evolution===<br /> At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region&lt;ref name=&quot;ref1&quot;/&gt;(Fig.7).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi&lt;ref name=&quot;ref2&quot;/&gt;,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain&lt;ref name=&quot;ref1&quot;/&gt;.<br /> [[File:1(DWA1).png|center|thumb|500px|Fig.7 Unrooted phylogenetic tree and domain architecture of DWA1 protein orthologs in vascular plants and microbial relatives(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> <br /> ==Labs working on this gene==<br /> *National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan,China<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Xiaoyi Zhu, Lizhong Xiong.（2013）Putative megaenzyme DWA1 plays essential roles in drought resistance by regulating stress-induced wax<br /> deposition in rice.PANS 110(44): 17790-17795.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Marahiel MA, Stachelhaus T, Mootz HD.(1997) Modular peptide synthetases involved in nonribosomal peptide synthesis.Chem Rev 97(7):2651–2674.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os04g0473900|<br /> Description = Adrenodoxin reductase family protein|<br /> Version = NM_001187049.1 GI:297723228 GeneID:9266980|<br /> Length = 9882 bp|<br /> Definition = Oryza sativa Japonica Group Os04g0473900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|<br /> AP = Chromosome 4:24097480..24107361|<br /> CDS = 24097480..24100068,24100784..24104053,24104151..24104329,24104410..24104769,24104855..24105296&lt;br&gt;,24105409..24105542,24105689..24105892,24107127..24107361|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008397:24097480..24107361<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:24097480..24107361<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactgatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttctggctcagaaaggcatctcaattccagattcttctgatcaggttggacttgttgtgattgcagaggttagagagggtaaggcagtctctaaagaggttgttaacaacatcaaagcacgtgtggtggaggagcatggtgtagctgttgcatctgtgaagcttatcaagcctagaaccatatgcaagacaacatctgggaagatacgaagatttgaatgcatgaggcagtttgttgacaatactcttagtttggctaagggcaaccatgtatctaagaaaaagggtctttttcggtctctgacaacaggaactggcatggagtcaaagaggtccctcttgaggcaaactgttgacctcaccattagccattggcccaaaagccaagttaaaaactccaatgaaataactgagtttctgacacaaatagtgtcagagcacactggcatttcaaaggataaaatttctctaactgacagtctaccttcgtatggttttgattctattgcagtggtacgagcagcccagaagctctcagattttcttggtgttcctgttggggccattgacatctttacagcaagctgcatttcggagcttgcaagcttcttggagaatttggtgcataagtcacaaacacagttggcaccttggcccaaaagcaaagtgaaaaactccaaggtgattatcgagtttctgacaaagattgtatcagatcaaacagggattccaaaggataaaatttctccgatggatagcctaccttcatatggttttgattcaattgcggtggtccaagcagcacagaaactctccgattttctcggtgtacctgttggagccattgacatatttacagctggttgcatttctgagctagctaccttcttggagaatttggcgcataagtcacagtcacagttggcacctggtgcatcttgctatattgaggatgagactcaagtggatcccatggatgccattagtccagaattcagtgttttgggtacgggcatcctgcaactgctggctcttacttatgtctgttttgtgctgctactcccagcatatcttgctagttcgacatacatgagcattttctctacagtcagtctggtcagatcaccattgctgagctatcttagttctttggttatggcacctattgtgtggatcttttatatctcgctcacttctttatcactgtctatcttggggaagtccttcctacagccaaattatgttctgatccctgatgtttcaatatggtcagttgactttgtgaaatggtgggcactaaacaaagcacaagcattagctgcaaagatgctagctgtgcatctaaagggcacaattttcttaaattattggttcaagatgcaaggagctcggattggttcatctgttgtcatcgacacagtagacataaccgatccatccctgttgacggttgcagatggtgcagttcttgcagaaggcgctcttgttcagggccatgaagtctgtaatgaagtgctgagttttaggcctatctggattggctgtgaggcttcgattggtccttatgcggtgcttcagaagggaactgttgtggaagatggagctgttgttccaccattgcagaagactggggctgggaaatcaacacgtcggactagcagaacttctgtaagcataaagaaagaagcagccaaagcaaacatgatacttgaacacctcgttagcatctacgcagttggcatcctgggtgctctgtccggtgcaatcgtctacacactatacactcacttgtccggcaaagcggcttcgcctctgcatttctcctttgcatgcattgctggtgcgttccactggttaccagctgccatcactgcctatgctgttattgtccaggagacacccacaagtgcactgtcatttgctctcttcactgccttcgccgacctgagctatggtgtcatcctcagtattctcaccagtataaccagcagagcccttgctgcaaagcctggaaccaagcagaatggcattgcgagtttgatccaccggcgcataaccatctctgctcatgtcaggtttgccaagatgctatctggaacagaagccttctgcgtgtacctgaggcttctcggagcaaagattggcaggcattgctctatccgagccatcaatccggtggcgaatcctgagctgatcagcgtcggtgatggcgtccatctaggtgacttctgcaatattgttccagggttctactccaaggggggattcaccagtgcagagattaaggtccaggagaacaccgtggttggcagtggcagcttgctcctccctggctgtgtcctccaggagaatgtcatcctcggcgcgctctccgtcgcgccggagaacgctgttctccggcgtggcggcgtctacgtcgggtcacagtctccggccatggtgaagaacaccttgcttgacgaggatgaacggatcgaagagatggatcaggcatacaagaagattgttggcaacctcgcggcgaacttggccatcaccaccatgaacgtcaagtcgaggtacttccaccgcatcggcgtgagcggccgcggcgtcctgaggatgtacgaggagataccgtcgtttccccggcacaagatcttcgcctccggcaagtccttcccggtgatcgtccgccacagcaacagcctgagcgccgacgacgacgcgaggctcgacgcccgcggcgccgcggtgcggatcctctcggacaacgacggcgaggcgcctctcctcgacctcacactcaagagcggcaaggcgttctacgcgcgcaccatcgccgacttcgccacctggctggtctgcggcctgccggcgagggaggagcaggtcaagcgctcgccgcacatccgcgacgccgtctggggctctctccggagcaccgactcgtacaccgtgctccactactactccaacatatgccggctgctgcggttcgacgacggcagggagatgtacgccaagttcaagctccgtcctgctgatccagacgtccccgaggactccggcaaggtggtgccgaggggcatcctgccgccggagaccggcgcgatccccagggacgaggatgacacgcgccctctgctcttcctcgccgacgacttccgccggagagtgggctcaccggacggggtgcgctacgtgttccagctgcagctcagggaggtgcccaccgacgccgccgcccgcgacgtcgccctcgactgcacccggccgtgggacgaggccgagttcccttacatcgacgtcggcgaggtcagcatcggccgcaacctcccgacggaggagacggagaagctcgagttcaacccgttcctccggtgccccgaggtggacgtcatcccggcgacgtcgtgcgcgcagagcgcgtccatcgaccacggccgctcgctggtctacgagatatgccagcgcctgcggaacggcgagccgctgcccgcgtcgtggcgagctttcctggagcagtcggacaccaagatcgacctgtcaggatgcccggtcgctgccgccgcgacacccacaaggtctaacgccggcgacgccacgaaggtgacgctggccaggacgtggtaccaggcgctctgggccacgctgtgccagccgctgctgcaaacgctggtgccgtactctgtcctgggcctcgtcatcttcctcccgctcaggggcctcctcgccgtcgccgcggccacccgcttcccgctctactggctcctcccggcgttctgggccgcgtcgggcgtcgccgccatggccacgtgcgcggcggcgaagtgggcgctggtcggcagcagggtcgacggcgacacggtgcacatctggtcgccggccgtgttcctggacacggtctggcaggccgtgcgcgccgccacggcggagtacttcgccgagctgacgcccgggtcggcgccgttcgcggcgtggatgcgggtgatgggcgcctccgtgtcccccggcgacggcgtgtacgtggactccatgggcgcgctgctgaacccggagatggtgcgcctggagcgcggcgccgccgtcgggcgggacgcgctgctgttcggccacgtgtacgagggcgaggccgggaaggtgaagttcggcgccgtgtccgtcggggaggacgggttcgtcggcagccgcgccgtggccatgcccagcgtcacggtggacgacggcggctgcctcgccgcgctcgggctggccatgaagggggagacggtgaagcacagcatgtaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MEERRATSPSRHSFCRDALGRRGGESGGGLVYIYRSPLRSPLQR HGLNREWRLERWRWRWRWEAATWRQRAAAHARIGEMDPKRSIDDQFSKLHPSLPVDTR IGIVGAGPSGLSAAYALAKLGYRNVTLFEKCHTVSGMCESIDIEEELGSDFEEMDTHK LSLIDSQTGNIRDLEVAEDYVSMVSLTLKLQDEANKSGRAGLHALSGLASDPTHEFLK QNGINSMPKSVAYGYTASGYGFVQDMPYAFIQEFTRTSMAGKIRRFKHGYMSMWERLS KSLPFEVFCDTQVLNVKRNSCGANVTIKNNNGEKQVLEFDKIILSGAVAFKNSKTYRS SSLTDGESEVVELNNLERELFSKVQTIDYYTTVVKINGFEHIPKGFYYFGEFMEDPTT IGHPVAMQRFFADTNIFLFWSYGNSADIKGSFVAKCVTDVVSSMGGNVQKVILQRRFK YFPHVSSKDMKDGFYERLESQLQGFQNTYYVGGLLAFELTERNASYSIMAVCKHFAID GEGELTPYVKRLFPLSHNRNPSPPRDLGELEGVEFPDLPSLDGYLQYWGTHKVTEKNV IYTWINEEGKLMNRRTYQELHGNASYIAQKLLTSTKPVIKPGDRVLLIHLPGLEFIDA FFGCIRAGVIPVPVLPPDPMQSGGQALLKVENISKMCNAVAILSTSSYHAAVRAGYIK NIVTLAKRVQKCSAQWPDIPWIHTDSWIKNYRRSSDSFNSDTVLFTKPQPSDLCFLQF TSGSTGDAKGVMITHEGLIHNVKTMKKRYRSTSKTVLVSWLPQYHDMGLIGGLFTALV SGGTSVLFSPMIFIRNPLLWLQTINDYHGTHSAGPNFAFELVIRRLEAEKNKVYDLSS MVFLMIAAEPVRQKTVRRFIELTQPFGLSEGVLAPGYGLAENCVYVTCAFGECKPVFI DWQGRVCCGYVEQDDTDTLIRIVDPDSLTEHQEDGVEGEIWISSPSSGVGYWGNSEMS QRTFFNQLKNHPNKKFTRTGDLGRTIDGNLFITGRIKDLIIVAGRNIYSADVEKTVES SSEVLRPGCCAVVGIPEEVLAQKGISIPDSSDQVGLVVIAEVREGKAVSKEVVNNIKA RVVEEHGVAVASVKLIKPRTICKTTSGKIRRFECMRQFVDNTLSLAKGNHVSKKKGLF RSLTTGTGMESKRSLLRQTVDLTISHWPKSQVKNSNEITEFLTQIVSEHTGISKDKIS LTDSLPSYGFDSIAVVRAAQKLSDFLGVPVGAIDIFTASCISELASFLENLVHKSQTQ LAPWPKSKVKNSKVIIEFLTKIVSDQTGIPKDKISPMDSLPSYGFDSIAVVQAAQKLS DFLGVPVGAIDIFTAGCISELATFLENLAHKSQSQLAPGASCYIEDETQVDPMDAISP EFSVLGTGILQLLALTYVCFVLLLPAYLASSTYMSIFSTVSLVRSPLLSYLSSLVMAP IVWIFYISLTSLSLSILGKSFLQPNYVLIPDVSIWSVDFVKWWALNKAQALAAKMLAV HLKGTIFLNYWFKMQGARIGSSVVIDTVDITDPSLLTVADGAVLAEGALVQGHEVCNE VLSFRPIWIGCEASIGPYAVLQKGTVVEDGAVVPPLQKTGAGKSTRRTSRTSVSIKKE AAKANMILEHLVSIYAVGILGALSGAIVYTLYTHLSGKAASPLHFSFACIAGAFHWLP AAITAYAVIVQETPTSALSFALFTAFADLSYGVILSILTSITSRALAAKPGTKQNGIA SLIHRRITISAHVRFAKMLSGTEAFCVYLRLLGAKIGRHCSIRAINPVANPELISVGD GVHLGDFCNIVPGFYSKGGFTSAEIKVQENTVVGSGSLLLPGCVLQENVILGALSVAP ENAVLRRGGVYVGSQSPAMVKNTLLDEDERIEEMDQAYKKIVGNLAANLAITTMNVKS RYFHRIGVSGRGVLRMYEEIPSFPRHKIFASGKSFPVIVRHSNSLSADDDARLDARGA AVRILSDNDGEAPLLDLTLKSGKAFYARTIADFATWLVCGLPAREEQVKRSPHIRDAV WGSLRSTDSYTVLHYYSNICRLLRFDDGREMYAKFKLRPADPDVPEDSGKVVPRGILP PETGAIPRDEDDTRPLLFLADDFRRRVGSPDGVRYVFQLQLREVPTDAAARDVALDCT RPWDEAEFPYIDVGEVSIGRNLPTEETEKLEFNPFLRCPEVDVIPATSCAQSASIDHG RSLVYEICQRLRNGEPLPASWRAFLEQSDTKIDLSGCPVAAAATPTRSNAGDATKVTL ARTWYQALWATLCQPLLQTLVPYSVLGLVIFLPLRGLLAVAAATRFPLYWLLPAFWAA SGVAAMATCAAAKWALVGSRVDGDTVHIWSPAVFLDTVWQAVRAATAEYFAELTPGSA PFAAWMRVMGASVSPGDGVYVDSMGALLNPEMVRLERGAAVGRDALLFGHVYEGEAGK VKFGAVSVGEDGFVGSRAVAMPSVTVDDGGCLAALGLAMKGETVKHSM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;7294..9882#3309..6578#3033..3211#2593..2952#2066..2507#1820..1953#1470..1673#1..235#atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggaggtatggattccccttccccactcgctgcgtgatgaaccgatgaaccgtagtacttgatgatatgcctgatttgcttcggcattgtgccgcttctctgttagctgggagtttgtggcgtgtcgtttaactgaagtagagatgtgatgatgatctgaggtgagcgccattttttttttttggctaactctgtttgttctattcagtgaatatgagctctcttttttgtcccctttttatcattctgcttctatgggtcgcggagcgttcttggttggtgcagtgcaactgcaatgtcagtatgcatgccatagtaatgtgatggtgggatgtaatgctcctctgcttgctcgagctctttcttggttgtctatctatcttggatgatttgatcaatgtactacttattatctgctgatagtacccggggtccatttgcgctagtattgaacttcttcttctcttataatttgctgaaatcgcgagattatcgcttaggtttatcaatcagaacattatcatgtttatgcttcggttcgttcggtactggcacatgtcaaataagcggaaattctgctactttttcggcttaccatgaatggcacccaacatagcgtggttgttttgtcatgttgaactagtacaaatttttgcaccttaatatttgctttccgtgcatatccaattaacctgcccatcaaacttcttttcagatagctattattggggaaaaatattttcccagcttcttttatagtgactagactgtttatacatgcctgcgtaattgcttccttttgatgagctagtacattatagaccttgattttgttttgatggtacgggttggttattggtgtccatataatgaaattgcaacttgcgaccactccatgatagttacctggtcattccttgttggttaataagctattatttcaaatatgaatgttcatcctacttgtcatattatagacatttgtcacaattcaagtgaggaagaaattctgtaattttctatgggctagttgctgcgttaactacaataatggggctgacatcctgaggttatagtgttcgattaccaagttctaatttattaagaggaatgcaagtcaacatgcatgcctagcccatttacttttaatgagtagtgtaacttctgttgcagaagtactaatgacagctttctatataaatttcacaggttgatttatcaaggacaacaattcgtcagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaaggtgaggaaactactcagcaaaattatacagtcatcattttgtgcacacttttagttatcagcaacattcgtatgtaggacgaatatatgatttgggaggacaagttatagcagcaaacagtgcccctgttataactcaccttgcagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggtgagttgttctagaataaaatttaaatttgaggtaactggataataagcatctaattatttataatattctctctatttcttgcatgcgtgttattcaataattcttacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactggtgagcatattttaaattcagtttggctggcattgaagctatagtatttcagttgccttattagtatgtatgatcttttgttcagatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaaggtatatatgttaataatatttctcctgttgcaaggtcttcttttttctcgtgataacattggtaatttcctgcattgcagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaaggtgagcaagaaataatggcgtaatatatctcaattattaattccaccttcatcaaatttcttagagaaattgtgcatatatttttccatacttgcagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagagg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ica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001187049.1 RefSeq:Os04g0473900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 4]]<br /> [[Category:Chromosome 4]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os04g0473900&diff=175256 2014-06-01T01:50:52Z

<p>Mayamei0424: /* Mutation */</p> <hr /> <div>''DWA1'' encodes an enzymatic megaprotein conserved in vascular plants，and ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''DWA1'' encodes an enzymatic megaprotein (2,391 aa in length) conserved in vascular plants, including an oxidoreductase-like domain, a prokaryotic nonribosomal peptide synthetase-like module, an AMP-binding domain, and an allene oxide synthase-like domain. The AMP-binding domain exhibits in vitro enzymatic activity in activating long-chain fatty acids to form acyl-CoA. Expression pattern analysis shows that ''DWA1'' is preferentially expressed in vascular tissues and epidermal layers and strongly induced by drought stress. The ''dwa1'' mutant was highly sensitive to drought stress compared to the WT and DWA1-functional complementation （DWA1-FC）plants restored drought resistance. Further dissections uncovers that the ''dwa1'' mutant was impaired in cuticular wax accumulation and significantly suppressed many wax-related genes expression under drought conditions and reduced levels of very-long-chain fatty acids. Conversely, plants overexpressing ''DWA1'' were significantly up-regulated many wax-related genes expression under drought conditions and elevated levels of very-long-chain fatty acids. The results suggest that ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice&lt;ref name=&quot;ref1&quot;/&gt;.<br /> <br /> ===Mutation===<br /> [[File:dwa1.jpg|center|thumb|200px|Fig.1 Screening of mutants(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa2.jpg|center|thumb|200px|Fig.2 phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa3.jpg|center|thumb|200px|Fig.3 functional complementation assay analysis(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa4.jpg|center|thumb|200px|Fig.4 Overexpression of ''DWA1'' analysis(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> '''(1)''' '''Screening of mutants(Identification of the drought-hypersensitive ''dwa1'' mutant in rice.)'''&lt;br&gt;<br /> ''dwa1'' mutant is observed by inserting T-DNA in the sixth exon of ''DWA1''(Fig.1A and B).RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1C and D).&lt;br&gt;<br /> '''(2)''' '''phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT'''&lt;br&gt;<br /> During the course of drought treatment, the mutant wilted and exhibited leaf rolling earlier than the WT. After severe drought treatment followed by rewatering, more than 90% of the WT plants survive, whereas the ''dwa1'' mutant plants are almost dead(Fig.2A and C). Water loss rate of mutant is faster than WT(Fig.2B).Furthermore, SEM analysis indicate that the deposition of vertical plate-like wax crystals on the ''dwa1'' leaf cuticle is severely reduced under normal and drought conditions in contrast to WT leaf(Fig.2D). The contents of individual FA components is obvious difference between the ''dwa1'' mutant and WT. Among them, VLCFAs are significantly reduced, whereas the levels of are slightly higher in the ''dwa1'' mutant(Fig.2E). The gene expression levels of wax-related genes are obviously different under drought treatment(Fig.2F)&lt;br&gt;<br /> '''(3) functional complementation assay analysis'''&lt;br&gt;<br /> DWA1-functional complementation(DWA1-FC)lines which is inserted the full-length genomic fragment of ''DWA1'' into the ''dwa1'' mutant restore drought resistance(Fig.3).&lt;br&gt;<br /> '''(4)''' '''Overexpression of ''DWA1'' analysis'''&lt;br&gt;<br /> There are three independent overexpression plants(U7,U9 and U10) and a negative control (U4),and there is no visual differences in the epicuticular wax load compared with the control (Fig. 4B).But the VLCFA constituents increase in the overexpression lines compared with WT. However, the contents of LCFAs are significantly reduced in the overexpression plants. The alkanes and primary alcohols showed no significant changes (Fig. 4C). Under normal growth conditions, the expression levels of wax-related genes enhance in the overexpression plants (Fig.4D).<br /> <br /> ===Expression===<br /> [[File:图片4.png|right|thumb|200px|Fig.5 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:图片5.png|right|thumb|200px|Fig.6 Drought-induced expression of ''DWA1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> '''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.5A).&lt;br&gt;<br /> '''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.5B1 and 2), axillary bud (Fig.5B3), young panicle (Fig.5B4),spikelet (Fig.5B5), stem (Fig.5B6), young panicle (Fig.5B7), pistil (Fig.5B8), stamen (Fig.5B9), young embryo (Fig.5B10), SEM picture of rice leaf cuticle (Fig.5B11), cross-sections of stem (Fig.5B12) and leaf (Fig.5B13),and semithin sections of leaf (Fig.5B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.&lt;br&gt;<br /> '''(3)'''What's more,after various phytohormones treatment,''DWA1'' expresses higher,such as abscisic acid, jasmonic acid, indole-3-acetic acid, and<br /> gibberellic acid (Fig.5C).&lt;br&gt;<br /> '''(4)'''Under drought treatment,GUS staining shows that''DWA1'' is induced.For example,in Fig.6B,GUS staining in leaf before (1 and 3) and after (2 and 4) drought stress is shown.&lt;br&gt;<br /> <br /> ===Evolution===<br /> At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region&lt;ref name=&quot;ref1&quot;/&gt;(Fig.7).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi&lt;ref name=&quot;ref2&quot;/&gt;,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain&lt;ref name=&quot;ref1&quot;/&gt;.<br /> [[File:1(DWA1).png|center|thumb|500px|Fig.7 Unrooted phylogenetic tree and domain architecture of DWA1 protein orthologs in vascular plants and microbial relatives(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> <br /> ==Labs working on this gene==<br /> *National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan,China<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Xiaoyi Zhu, Lizhong Xiong.（2013）Putative megaenzyme DWA1 plays essential roles in drought resistance by regulating stress-induced wax<br /> deposition in rice.PANS 110(44): 17790-17795.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Marahiel MA, Stachelhaus T, Mootz HD.(1997) Modular peptide synthetases involved in nonribosomal peptide synthesis.Chem Rev 97(7):2651–2674.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os04g0473900|<br /> Description = Adrenodoxin reductase family protein|<br /> Version = NM_001187049.1 GI:297723228 GeneID:9266980|<br /> Length = 9882 bp|<br /> Definition = Oryza sativa Japonica Group Os04g0473900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|<br /> AP = Chromosome 4:24097480..24107361|<br /> CDS = 24097480..24100068,24100784..24104053,24104151..24104329,24104410..24104769,24104855..24105296&lt;br&gt;,24105409..24105542,24105689..24105892,24107127..24107361|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008397:24097480..24107361<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:24097480..24107361<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactgatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttctggctcagaaaggcatctcaattccagattcttctgatcaggttggacttgttgtgattgcagaggttagagagggtaaggcagtctctaaagaggttgttaacaacatcaaagcacgtgtggtggaggagcatggtgtagctgttgcatctgtgaagcttatcaagcctagaaccatatgcaagacaacatctgggaagatacgaagatttgaatgcatgaggcagtttgttgacaatactcttagtttggctaagggcaaccatgtatctaagaaaaagggtctttttcggtctctgacaacaggaactggcatggagtcaaagaggtccctcttgaggcaaactgttgacctcaccattagccattggcccaaaagccaagttaaaaactccaatgaaataactgagtttctgacacaaatagtgtcagagcacactggcatttcaaaggataaaatttctctaactgacagtctaccttcgtatggttttgattctattgcagtggtacgagcagcccagaagctctcagattttcttggtgttcctgttggggccattgacatctttacagcaagctgcatttcggagcttgcaagcttcttggagaatttggtgcataagtcacaaacacagttggcaccttggcccaaaagcaaagtgaaaaactccaaggtgattatcgagtttctgacaaagattgtatcagatcaaacagggattccaaaggataaaatttctccgatggatagcctaccttcatatggttttgattcaattgcggtggtccaagcagcacagaaactctccgattttctcggtgtacctgttggagccattgacatatttacagctggttgcatttctgagctagctaccttcttggagaatttggcgcataagtcacagtcacagttggcacctggtgcatcttgctatattgaggatgagactcaagtggatcccatggatgccattagtccagaattcagtgttttgggtacgggcatcctgcaactgctggctcttacttatgtctgttttgtgctgctactcccagcatatcttgctagttcgacatacatgagcattttctctacagtcagtctggtcagatcaccattgctgagctatcttagttctttggttatggcacctattgtgtggatcttttatatctcgctcacttctttatcactgtctatcttggggaagtccttcctacagccaaattatgttctgatccctgatgtttcaatatggtcagttgactttgtgaaatggtgggcactaaacaaagcacaagcattagctgcaaagatgctagctgtgcatctaaagggcacaattttcttaaattattggttcaagatgcaaggagctcggattggttcatctgttgtcatcgacacagtagacataaccgatccatccctgttgacggttgcagatggtgcagttcttgcagaaggcgctcttgttcagggccatgaagtctgtaatgaagtgctgagttttaggcctatctggattggctgtgaggcttcgattggtccttatgcggtgcttcagaagggaactgttgtggaagatggagctgttgttccaccattgcagaagactggggctgggaaatcaacacgtcggactagcagaacttctgtaagcataaagaaagaagcagccaaagcaaacatgatacttgaacacctcgttagcatctacgcagttggcatcctgggtgctctgtccggtgcaatcgtctacacactatacactcacttgtccggcaaagcggcttcgcctctgcatttctcctttgcatgcattgctggtgcgttccactggttaccagctgccatcactgcctatgctgttattgtccaggagacacccacaagtgcactgtcatttgctctcttcactgccttcgccgacctgagctatggtgtcatcctcagtattctcaccagtataaccagcagagcccttgctgcaaagcctggaaccaagcagaatggcattgcgagtttgatccaccggcgcataaccatctctgctcatgtcaggtttgccaagatgctatctggaacagaagccttctgcgtgtacctgaggcttctcggagcaaagattggcaggcattgctctatccgagccatcaatccggtggcgaatcctgagctgatcagcgtcggtgatggcgtccatctaggtgacttctgcaatattgttccagggttctactccaaggggggattcaccagtgcagagattaaggtccaggagaacaccgtggttggcagtggcagcttgctcctccctggctgtgtcctccaggagaatgtcatcctcggcgcgctctccgtcgcgccggagaacgctgttctccggcgtggcggcgtctacgtcgggtcacagtctccggccatggtgaagaacaccttgcttgacgaggatgaacggatcgaagagatggatcaggcatacaagaagattgttggcaacctcgcggcgaacttggccatcaccaccatgaacgtcaagtcgaggtacttccaccgcatcggcgtgagcggccgcggcgtcctgaggatgtacgaggagataccgtcgtttccccggcacaagatcttcgcctccggcaagtccttcccggtgatcgtccgccacagcaacagcctgagcgccgacgacgacgcgaggctcgacgcccgcggcgccgcggtgcggatcctctcggacaacgacggcgaggcgcctctcctcgacctcacactcaagagcggcaaggcgttctacgcgcgcaccatcgccgacttcgccacctggctggtctgcggcctgccggcgagggaggagcaggtcaagcgctcgccgcacatccgcgacgccgtctggggctctctccggagcaccgactcgtacaccgtgctccactactactccaacatatgccggctgctgcggttcgacgacggcagggagatgtacgccaagttcaagctccgtcctgctgatccagacgtccccgaggactccggcaaggtggtgccgaggggcatcctgccgccggagaccggcgcgatccccagggacgaggatgacacgcgccctctgctcttcctcgccgacgacttccgccggagagtgggctcaccggacggggtgcgctacgtgttccagctgcagctcagggaggtgcccaccgacgccgccgcccgcgacgtcgccctcgactgcacccggccgtgggacgaggccgagttcccttacatcgacgtcggcgaggtcagcatcggccgcaacctcccgacggaggagacggagaagctcgagttcaacccgttcctccggtgccccgaggtggacgtcatcccggcgacgtcgtgcgcgcagagcgcgtccatcgaccacggccgctcgctggtctacgagatatgccagcgcctgcggaacggcgagccgctgcccgcgtcgtggcgagctttcctggagcagtcggacaccaagatcgacctgtcaggatgcccggtcgctgccgccgcgacacccacaaggtctaacgccggcgacgccacgaaggtgacgctggccaggacgtggtaccaggcgctctgggccacgctgtgccagccgctgctgcaaacgctggtgccgtactctgtcctgggcctcgtcatcttcctcccgctcaggggcctcctcgccgtcgccgcggccacccgcttcccgctctactggctcctcccggcgttctgggccgcgtcgggcgtcgccgccatggccacgtgcgcggcggcgaagtgggcgctggtcggcagcagggtcgacggcgacacggtgcacatctggtcgccggccgtgttcctggacacggtctggcaggccgtgcgcgccgccacggcggagtacttcgccgagctgacgcccgggtcggcgccgttcgcggcgtggatgcgggtgatgggcgcctccgtgtcccccggcgacggcgtgtacgtggactccatgggcgcgctgctgaacccggagatggtgcgcctggagcgcggcgccgccgtcgggcgggacgcgctgctgttcggccacgtgtacgagggcgaggccgggaaggtgaagttcggcgccgtgtccgtcggggaggacgggttcgtcggcagccgcgccgtggccatgcccagcgtcacggtggacgacggcggctgcctcgccgcgctcgggctggccatgaagggggagacggtgaagcacagcatgtaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MEERRATSPSRHSFCRDALGRRGGESGGGLVYIYRSPLRSPLQR HGLNREWRLERWRWRWRWEAATWRQRAAAHARIGEMDPKRSIDDQFSKLHPSLPVDTR IGIVGAGPSGLSAAYALAKLGYRNVTLFEKCHTVSGMCESIDIEEELGSDFEEMDTHK LSLIDSQTGNIRDLEVAEDYVSMVSLTLKLQDEANKSGRAGLHALSGLASDPTHEFLK QNGINSMPKSVAYGYTASGYGFVQDMPYAFIQEFTRTSMAGKIRRFKHGYMSMWERLS KSLPFEVFCDTQVLNVKRNSCGANVTIKNNNGEKQVLEFDKIILSGAVAFKNSKTYRS SSLTDGESEVVELNNLERELFSKVQTIDYYTTVVKINGFEHIPKGFYYFGEFMEDPTT IGHPVAMQRFFADTNIFLFWSYGNSADIKGSFVAKCVTDVVSSMGGNVQKVILQRRFK YFPHVSSKDMKDGFYERLESQLQGFQNTYYVGGLLAFELTERNASYSIMAVCKHFAID GEGELTPYVKRLFPLSHNRNPSPPRDLGELEGVEFPDLPSLDGYLQYWGTHKVTEKNV IYTWINEEGKLMNRRTYQELHGNASYIAQKLLTSTKPVIKPGDRVLLIHLPGLEFIDA FFGCIRAGVIPVPVLPPDPMQSGGQALLKVENISKMCNAVAILSTSSYHAAVRAGYIK NIVTLAKRVQKCSAQWPDIPWIHTDSWIKNYRRSSDSFNSDTVLFTKPQPSDLCFLQF TSGSTGDAKGVMITHEGLIHNVKTMKKRYRSTSKTVLVSWLPQYHDMGLIGGLFTALV SGGTSVLFSPMIFIRNPLLWLQTINDYHGTHSAGPNFAFELVIRRLEAEKNKVYDLSS MVFLMIAAEPVRQKTVRRFIELTQPFGLSEGVLAPGYGLAENCVYVTCAFGECKPVFI DWQGRVCCGYVEQDDTDTLIRIVDPDSLTEHQEDGVEGEIWISSPSSGVGYWGNSEMS QRTFFNQLKNHPNKKFTRTGDLGRTIDGNLFITGRIKDLIIVAGRNIYSADVEKTVES SSEVLRPGCCAVVGIPEEVLAQKGISIPDSSDQVGLVVIAEVREGKAVSKEVVNNIKA RVVEEHGVAVASVKLIKPRTICKTTSGKIRRFECMRQFVDNTLSLAKGNHVSKKKGLF RSLTTGTGMESKRSLLRQTVDLTISHWPKSQVKNSNEITEFLTQIVSEHTGISKDKIS LTDSLPSYGFDSIAVVRAAQKLSDFLGVPVGAIDIFTASCISELASFLENLVHKSQTQ LAPWPKSKVKNSKVIIEFLTKIVSDQTGIPKDKISPMDSLPSYGFDSIAVVQAAQKLS DFLGVPVGAIDIFTAGCISELATFLENLAHKSQSQLAPGASCYIEDETQVDPMDAISP EFSVLGTGILQLLALTYVCFVLLLPAYLASSTYMSIFSTVSLVRSPLLSYLSSLVMAP IVWIFYISLTSLSLSILGKSFLQPNYVLIPDVSIWSVDFVKWWALNKAQALAAKMLAV HLKGTIFLNYWFKMQGARIGSSVVIDTVDITDPSLLTVADGAVLAEGALVQGHEVCNE VLSFRPIWIGCEASIGPYAVLQKGTVVEDGAVVPPLQKTGAGKSTRRTSRTSVSIKKE AAKANMILEHLVSIYAVGILGALSGAIVYTLYTHLSGKAASPLHFSFACIAGAFHWLP AAITAYAVIVQETPTSALSFALFTAFADLSYGVILSILTSITSRALAAKPGTKQNGIA SLIHRRITISAHVRFAKMLSGTEAFCVYLRLLGAKIGRHCSIRAINPVANPELISVGD GVHLGDFCNIVPGFYSKGGFTSAEIKVQENTVVGSGSLLLPGCVLQENVILGALSVAP ENAVLRRGGVYVGSQSPAMVKNTLLDEDERIEEMDQAYKKIVGNLAANLAITTMNVKS RYFHRIGVSGRGVLRMYEEIPSFPRHKIFASGKSFPVIVRHSNSLSADDDARLDARGA AVRILSDNDGEAPLLDLTLKSGKAFYARTIADFATWLVCGLPAREEQVKRSPHIRDAV WGSLRSTDSYTVLHYYSNICRLLRFDDGREMYAKFKLRPADPDVPEDSGKVVPRGILP PETGAIPRDEDDTRPLLFLADDFRRRVGSPDGVRYVFQLQLREVPTDAAARDVALDCT RPWDEAEFPYIDVGEVSIGRNLPTEETEKLEFNPFLRCPEVDVIPATSCAQSASIDHG RSLVYEICQRLRNGEPLPASWRAFLEQSDTKIDLSGCPVAAAATPTRSNAGDATKVTL ARTWYQALWATLCQPLLQTLVPYSVLGLVIFLPLRGLLAVAAATRFPLYWLLPAFWAA SGVAAMATCAAAKWALVGSRVDGDTVHIWSPAVFLDTVWQAVRAATAEYFAELTPGSA PFAAWMRVMGASVSPGDGVYVDSMGALLNPEMVRLERGAAVGRDALLFGHVYEGEAGK VKFGAVSVGEDGFVGSRAVAMPSVTVDDGGCLAALGLAMKGETVKHSM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;7294..9882#3309..6578#3033..3211#2593..2952#2066..2507#1820..1953#1470..1673#1..235#atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggaggtatggattccccttccccactcgctgcgtgatgaaccgatgaaccgtagtacttgatgatatgcctgatttgcttcggcattgtgccgcttctctgttagctgggagtttgtggcgtgtcgtttaactgaagtagagatgtgatgatgatctgaggtgagcgccattttttttttttggctaactctgtttgttctattcagtgaatatgagctctcttttttgtcccctttttatcattctgcttctatgggtcgcggagcgttcttggttggtgcagtgcaactgcaatgtcagtatgcatgccatagtaatgtgatggtgggatgtaatgctcctctgcttgctcgagctctttcttggttgtctatctatcttggatgatttgatcaatgtactacttattatctgctgatagtacccggggtccatttgcgctagtattgaacttcttcttctcttataatttgctgaaatcgcgagattatcgcttaggtttatcaatcagaacattatcatgtttatgcttcggttcgttcggtactggcacatgtcaaataagcggaaattctgctactttttcggcttaccatgaatggcacccaacatagcgtggttgttttgtcatgttgaactagtacaaatttttgcaccttaatatttgctttccgtgcatatccaattaacctgcccatcaaacttcttttcagatagctattattggggaaaaatattttcccagcttcttttatagtgactagactgtttatacatgcctgcgtaattgcttccttttgatgagctagtacattatagaccttgattttgttttgatggtacgggttggttattggtgtccatataatgaaattgcaacttgcgaccactccatgatagttacctggtcattccttgttggttaataagctattatttcaaatatgaatgttcatcctacttgtcatattatagacatttgtcacaattcaagtgaggaagaaattctgtaattttctatgggctagttgctgcgttaactacaataatggggctgacatcctgaggttatagtgttcgattaccaagttctaatttattaagaggaatgcaagtcaacatgcatgcctagcccatttacttttaatgagtagtgtaacttctgttgcagaagtactaatgacagctttctatataaatttcacaggttgatttatcaaggacaacaattcgtcagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaaggtgaggaaactactcagcaaaattatacagtcatcattttgtgcacacttttagttatcagcaacattcgtatgtaggacgaatatatgatttgggaggacaagttatagcagcaaacagtgcccctgttataactcaccttgcagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggtgagttgttctagaataaaatttaaatttgaggtaactggataataagcatctaattatttataatattctctctatttcttgcatgcgtgttattcaataattcttacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactggtgagcatattttaaattcagtttggctggcattgaagctatagtatttcagttgccttattagtatgtatgatcttttgttcagatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaaggtatatatgttaataatatttctcctgttgcaaggtcttcttttttctcgtgataacattggtaatttcctgcattgcagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaaggtgagcaagaaataatggcgtaatatatctcaattattaattccaccttcatcaaatttcttagagaaattgtgcatatatttttccatacttgcagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagagg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ica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001187049.1 RefSeq:Os04g0473900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 4]]<br /> [[Category:Chromosome 4]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os04g0473900&diff=175255 2014-06-01T01:49:13Z

<p>Mayamei0424: /* Evolution */</p> <hr /> <div>''DWA1'' encodes an enzymatic megaprotein conserved in vascular plants，and ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''DWA1'' encodes an enzymatic megaprotein (2,391 aa in length) conserved in vascular plants, including an oxidoreductase-like domain, a prokaryotic nonribosomal peptide synthetase-like module, an AMP-binding domain, and an allene oxide synthase-like domain. The AMP-binding domain exhibits in vitro enzymatic activity in activating long-chain fatty acids to form acyl-CoA. Expression pattern analysis shows that ''DWA1'' is preferentially expressed in vascular tissues and epidermal layers and strongly induced by drought stress. The ''dwa1'' mutant was highly sensitive to drought stress compared to the WT and DWA1-functional complementation （DWA1-FC）plants restored drought resistance. Further dissections uncovers that the ''dwa1'' mutant was impaired in cuticular wax accumulation and significantly suppressed many wax-related genes expression under drought conditions and reduced levels of very-long-chain fatty acids. Conversely, plants overexpressing ''DWA1'' were significantly up-regulated many wax-related genes expression under drought conditions and elevated levels of very-long-chain fatty acids. The results suggest that ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice&lt;ref name=&quot;ref1&quot;/&gt;.<br /> <br /> ===Mutation===<br /> [[File:dwa1.jpg|right|thumb|200px|Fig.1 Screening of mutants(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa2.jpg|right|thumb|200px|Fig.2 phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa3.jpg|right|thumb|200px|Fig.3 functional complementation assay analysis(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa4.jpg|right|thumb|200px|Fig.4 Overexpression of ''DWA1'' analysis(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> '''(1)''' '''Screening of mutants(Identification of the drought-hypersensitive ''dwa1'' mutant in rice.)'''&lt;br&gt;<br /> ''dwa1'' mutant is observed by inserting T-DNA in the sixth exon of ''DWA1''(Fig.1A and B).RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1C and D).&lt;br&gt;<br /> '''(2)''' '''phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT'''&lt;br&gt;<br /> During the course of drought treatment, the mutant wilted and exhibited leaf rolling earlier than the WT. After severe drought treatment followed by rewatering, more than 90% of the WT plants survive, whereas the ''dwa1'' mutant plants are almost dead(Fig.2A and C). Water loss rate of mutant is faster than WT(Fig.2B).Furthermore, SEM analysis indicate that the deposition of vertical plate-like wax crystals on the ''dwa1'' leaf cuticle is severely reduced under normal and drought conditions in contrast to WT leaf(Fig.2D). The contents of individual FA components is obvious difference between the ''dwa1'' mutant and WT. Among them, VLCFAs are significantly reduced, whereas the levels of are slightly higher in the ''dwa1'' mutant(Fig.2E). The gene expression levels of wax-related genes are obviously different under drought treatment(Fig.2F)&lt;br&gt;<br /> '''(3) functional complementation assay analysis'''&lt;br&gt;<br /> DWA1-functional complementation(DWA1-FC)lines which is inserted the full-length genomic fragment of ''DWA1'' into the ''dwa1'' mutant restore drought resistance(Fig.3).&lt;br&gt;<br /> '''(4)''' '''Overexpression of ''DWA1'' analysis'''&lt;br&gt;<br /> There are three independent overexpression plants(U7,U9 and U10) and a negative control (U4),and there is no visual differences in the epicuticular wax load compared with the control (Fig. 4B).But the VLCFA constituents increase in the overexpression lines compared with WT. However, the contents of LCFAs are significantly reduced in the overexpression plants. The alkanes and primary alcohols showed no significant changes (Fig. 4C). Under normal growth conditions, the expression levels of wax-related genes enhance in the overexpression plants (Fig.4D).<br /> <br /> ===Expression===<br /> [[File:图片4.png|right|thumb|200px|Fig.5 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:图片5.png|right|thumb|200px|Fig.6 Drought-induced expression of ''DWA1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> '''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.5A).&lt;br&gt;<br /> '''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.5B1 and 2), axillary bud (Fig.5B3), young panicle (Fig.5B4),spikelet (Fig.5B5), stem (Fig.5B6), young panicle (Fig.5B7), pistil (Fig.5B8), stamen (Fig.5B9), young embryo (Fig.5B10), SEM picture of rice leaf cuticle (Fig.5B11), cross-sections of stem (Fig.5B12) and leaf (Fig.5B13),and semithin sections of leaf (Fig.5B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.&lt;br&gt;<br /> '''(3)'''What's more,after various phytohormones treatment,''DWA1'' expresses higher,such as abscisic acid, jasmonic acid, indole-3-acetic acid, and<br /> gibberellic acid (Fig.5C).&lt;br&gt;<br /> '''(4)'''Under drought treatment,GUS staining shows that''DWA1'' is induced.For example,in Fig.6B,GUS staining in leaf before (1 and 3) and after (2 and 4) drought stress is shown.&lt;br&gt;<br /> <br /> ===Evolution===<br /> At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region&lt;ref name=&quot;ref1&quot;/&gt;(Fig.7).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi&lt;ref name=&quot;ref2&quot;/&gt;,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain&lt;ref name=&quot;ref1&quot;/&gt;.<br /> [[File:1(DWA1).png|center|thumb|500px|Fig.7 Unrooted phylogenetic tree and domain architecture of DWA1 protein orthologs in vascular plants and microbial relatives(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> <br /> ==Labs working on this gene==<br /> *National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan,China<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Xiaoyi Zhu, Lizhong Xiong.（2013）Putative megaenzyme DWA1 plays essential roles in drought resistance by regulating stress-induced wax<br /> deposition in rice.PANS 110(44): 17790-17795.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Marahiel MA, Stachelhaus T, Mootz HD.(1997) Modular peptide synthetases involved in nonribosomal peptide synthesis.Chem Rev 97(7):2651–2674.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os04g0473900|<br /> Description = Adrenodoxin reductase family protein|<br /> Version = NM_001187049.1 GI:297723228 GeneID:9266980|<br /> Length = 9882 bp|<br /> Definition = Oryza sativa Japonica Group Os04g0473900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|<br /> AP = Chromosome 4:24097480..24107361|<br /> CDS = 24097480..24100068,24100784..24104053,24104151..24104329,24104410..24104769,24104855..24105296&lt;br&gt;,24105409..24105542,24105689..24105892,24107127..24107361|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008397:24097480..24107361<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:24097480..24107361<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactgatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttctggctcagaaaggcatctcaattccagattcttctgatcaggttggacttgttgtgattgcagaggttagagagggtaaggcagtctctaaagaggttgttaacaacatcaaagcacgtgtggtggaggagcatggtgtagctgttgcatctgtgaagcttatcaagcctagaaccatatgcaagacaacatctgggaagatacgaagatttgaatgcatgaggcagtttgttgacaatactcttagtttggctaagggcaaccatgtatctaagaaaaagggtctttttcggtctctgacaacaggaactggcatggagtcaaagaggtccctcttgaggcaaactgttgacctcaccattagccattggcccaaaagccaagttaaaaactccaatgaaataactgagtttctgacacaaatagtgtcagagcacactggcatttcaaaggataaaatttctctaactgacagtctaccttcgtatggttttgattctattgcagtggtacgagcagcccagaagctctcagattttcttggtgttcctgttggggccattgacatctttacagcaagctgcatttcggagcttgcaagcttcttggagaatttggtgcataagtcacaaacacagttggcaccttggcccaaaagcaaagtgaaaaactccaaggtgattatcgagtttctgacaaagattgtatcagatcaaacagggattccaaaggataaaatttctccgatggatagcctaccttcatatggttttgattcaattgcggtggtccaagcagcacagaaactctccgattttctcggtgtacctgttggagccattgacatatttacagctggttgcatttctgagctagctaccttcttggagaatttggcgcataagtcacagtcacagttggcacctggtgcatcttgctatattgaggatgagactcaagtggatcccatggatgccattagtccagaattcagtgttttgggtacgggcatcctgcaactgctggctcttacttatgtctgttttgtgctgctactcccagcatatcttgctagttcgacatacatgagcattttctctacagtcagtctggtcagatcaccattgctgagctatcttagttctttggttatggcacctattgtgtggatcttttatatctcgctcacttctttatcactgtctatcttggggaagtccttcctacagccaaattatgttctgatccctgatgtttcaatatggtcagttgactttgtgaaatggtgggcactaaacaaagcacaagcattagctgcaaagatgctagctgtgcatctaaagggcacaattttcttaaattattggttcaagatgcaaggagctcggattggttcatctgttgtcatcgacacagtagacataaccgatccatccctgttgacggttgcagatggtgcagttcttgcagaaggcgctcttgttcagggccatgaagtctgtaatgaagtgctgagttttaggcctatctggattggctgtgaggcttcgattggtccttatgcggtgcttcagaagggaactgttgtggaagatggagctgttgttccaccattgcagaagactggggctgggaaatcaacacgtcggactagcagaacttctgtaagcataaagaaagaagcagccaaagcaaacatgatacttgaacacctcgttagcatctacgcagttggcatcctgggtgctctgtccggtgcaatcgtctacacactatacactcacttgtccggcaaagcggcttcgcctctgcatttctcctttgcatgcattgctggtgcgttccactggttaccagctgccatcactgcctatgctgttattgtccaggagacacccacaagtgcactgtcatttgctctcttcactgccttcgccgacctgagctatggtgtcatcctcagtattctcaccagtataaccagcagagcccttgctgcaaagcctggaaccaagcagaatggcattgcgagtttgatccaccggcgcataaccatctctgctcatgtcaggtttgccaagatgctatctggaacagaagccttctgcgtgtacctgaggcttctcggagcaaagattggcaggcattgctctatccgagccatcaatccggtggcgaatcctgagctgatcagcgtcggtgatggcgtccatctaggtgacttctgcaatattgttccagggttctactccaaggggggattcaccagtgcagagattaaggtccaggagaacaccgtggttggcagtggcagcttgctcctccctggctgtgtcctccaggagaatgtcatcctcggcgcgctctccgtcgcgccggagaacgctgttctccggcgtggcggcgtctacgtcgggtcacagtctccggccatggtgaagaacaccttgcttgacgaggatgaacggatcgaagagatggatcaggcatacaagaagattgttggcaacctcgcggcgaacttggccatcaccaccatgaacgtcaagtcgaggtacttccaccgcatcggcgtgagcggccgcggcgtcctgaggatgtacgaggagataccgtcgtttccccggcacaagatcttcgcctccggcaagtccttcccggtgatcgtccgccacagcaacagcctgagcgccgacgacgacgcgaggctcgacgcccgcggcgccgcggtgcggatcctctcggacaacgacggcgaggcgcctctcctcgacctcacactcaagagcggcaaggcgttctacgcgcgcaccatcgccgacttcgccacctggctggtctgcggcctgccggcgagggaggagcaggtcaagcgctcgccgcacatccgcgacgccgtctggggctctctccggagcaccgactcgtacaccgtgctccactactactccaacatatgccggctgctgcggttcgacgacggcagggagatgtacgccaagttcaagctccgtcctgctgatccagacgtccccgaggactccggcaaggtggtgccgaggggcatcctgccgccggagaccggcgcgatccccagggacgaggatgacacgcgccctctgctcttcctcgccgacgacttccgccggagagtgggctcaccggacggggtgcgctacgtgttccagctgcagctcagggaggtgcccaccgacgccgccgcccgcgacgtcgccctcgactgcacccggccgtgggacgaggccgagttcccttacatcgacgtcggcgaggtcagcatcggccgcaacctcccgacggaggagacggagaagctcgagttcaacccgttcctccggtgccccgaggtggacgtcatcccggcgacgtcgtgcgcgcagagcgcgtccatcgaccacggccgctcgctggtctacgagatatgccagcgcctgcggaacggcgagccgctgcccgcgtcgtggcgagctttcctggagcagtcggacaccaagatcgacctgtcaggatgcccggtcgctgccgccgcgacacccacaaggtctaacgccggcgacgccacgaaggtgacgctggccaggacgtggtaccaggcgctctgggccacgctgtgccagccgctgctgcaaacgctggtgccgtactctgtcctgggcctcgtcatcttcctcccgctcaggggcctcctcgccgtcgccgcggccacccgcttcccgctctactggctcctcccggcgttctgggccgcgtcgggcgtcgccgccatggccacgtgcgcggcggcgaagtgggcgctggtcggcagcagggtcgacggcgacacggtgcacatctggtcgccggccgtgttcctggacacggtctggcaggccgtgcgcgccgccacggcggagtacttcgccgagctgacgcccgggtcggcgccgttcgcggcgtggatgcgggtgatgggcgcctccgtgtcccccggcgacggcgtgtacgtggactccatgggcgcgctgctgaacccggagatggtgcgcctggagcgcggcgccgccgtcgggcgggacgcgctgctgttcggccacgtgtacgagggcgaggccgggaaggtgaagttcggcgccgtgtccgtcggggaggacgggttcgtcggcagccgcgccgtggccatgcccagcgtcacggtggacgacggcggctgcctcgccgcgctcgggctggccatgaagggggagacggtgaagcacagcatgtaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MEERRATSPSRHSFCRDALGRRGGESGGGLVYIYRSPLRSPLQR HGLNREWRLERWRWRWRWEAATWRQRAAAHARIGEMDPKRSIDDQFSKLHPSLPVDTR IGIVGAGPSGLSAAYALAKLGYRNVTLFEKCHTVSGMCESIDIEEELGSDFEEMDTHK LSLIDSQTGNIRDLEVAEDYVSMVSLTLKLQDEANKSGRAGLHALSGLASDPTHEFLK QNGINSMPKSVAYGYTASGYGFVQDMPYAFIQEFTRTSMAGKIRRFKHGYMSMWERLS KSLPFEVFCDTQVLNVKRNSCGANVTIKNNNGEKQVLEFDKIILSGAVAFKNSKTYRS SSLTDGESEVVELNNLERELFSKVQTIDYYTTVVKINGFEHIPKGFYYFGEFMEDPTT IGHPVAMQRFFADTNIFLFWSYGNSADIKGSFVAKCVTDVVSSMGGNVQKVILQRRFK YFPHVSSKDMKDGFYERLESQLQGFQNTYYVGGLLAFELTERNASYSIMAVCKHFAID GEGELTPYVKRLFPLSHNRNPSPPRDLGELEGVEFPDLPSLDGYLQYWGTHKVTEKNV IYTWINEEGKLMNRRTYQELHGNASYIAQKLLTSTKPVIKPGDRVLLIHLPGLEFIDA FFGCIRAGVIPVPVLPPDPMQSGGQALLKVENISKMCNAVAILSTSSYHAAVRAGYIK NIVTLAKRVQKCSAQWPDIPWIHTDSWIKNYRRSSDSFNSDTVLFTKPQPSDLCFLQF TSGSTGDAKGVMITHEGLIHNVKTMKKRYRSTSKTVLVSWLPQYHDMGLIGGLFTALV SGGTSVLFSPMIFIRNPLLWLQTINDYHGTHSAGPNFAFELVIRRLEAEKNKVYDLSS MVFLMIAAEPVRQKTVRRFIELTQPFGLSEGVLAPGYGLAENCVYVTCAFGECKPVFI DWQGRVCCGYVEQDDTDTLIRIVDPDSLTEHQEDGVEGEIWISSPSSGVGYWGNSEMS QRTFFNQLKNHPNKKFTRTGDLGRTIDGNLFITGRIKDLIIVAGRNIYSADVEKTVES SSEVLRPGCCAVVGIPEEVLAQKGISIPDSSDQVGLVVIAEVREGKAVSKEVVNNIKA RVVEEHGVAVASVKLIKPRTICKTTSGKIRRFECMRQFVDNTLSLAKGNHVSKKKGLF RSLTTGTGMESKRSLLRQTVDLTISHWPKSQVKNSNEITEFLTQIVSEHTGISKDKIS LTDSLPSYGFDSIAVVRAAQKLSDFLGVPVGAIDIFTASCISELASFLENLVHKSQTQ LAPWPKSKVKNSKVIIEFLTKIVSDQTGIPKDKISPMDSLPSYGFDSIAVVQAAQKLS DFLGVPVGAIDIFTAGCISELATFLENLAHKSQSQLAPGASCYIEDETQVDPMDAISP EFSVLGTGILQLLALTYVCFVLLLPAYLASSTYMSIFSTVSLVRSPLLSYLSSLVMAP IVWIFYISLTSLSLSILGKSFLQPNYVLIPDVSIWSVDFVKWWALNKAQALAAKMLAV HLKGTIFLNYWFKMQGARIGSSVVIDTVDITDPSLLTVADGAVLAEGALVQGHEVCNE VLSFRPIWIGCEASIGPYAVLQKGTVVEDGAVVPPLQKTGAGKSTRRTSRTSVSIKKE AAKANMILEHLVSIYAVGILGALSGAIVYTLYTHLSGKAASPLHFSFACIAGAFHWLP AAITAYAVIVQETPTSALSFALFTAFADLSYGVILSILTSITSRALAAKPGTKQNGIA SLIHRRITISAHVRFAKMLSGTEAFCVYLRLLGAKIGRHCSIRAINPVANPELISVGD GVHLGDFCNIVPGFYSKGGFTSAEIKVQENTVVGSGSLLLPGCVLQENVILGALSVAP ENAVLRRGGVYVGSQSPAMVKNTLLDEDERIEEMDQAYKKIVGNLAANLAITTMNVKS RYFHRIGVSGRGVLRMYEEIPSFPRHKIFASGKSFPVIVRHSNSLSADDDARLDARGA AVRILSDNDGEAPLLDLTLKSGKAFYARTIADFATWLVCGLPAREEQVKRSPHIRDAV WGSLRSTDSYTVLHYYSNICRLLRFDDGREMYAKFKLRPADPDVPEDSGKVVPRGILP PETGAIPRDEDDTRPLLFLADDFRRRVGSPDGVRYVFQLQLREVPTDAAARDVALDCT RPWDEAEFPYIDVGEVSIGRNLPTEETEKLEFNPFLRCPEVDVIPATSCAQSASIDHG RSLVYEICQRLRNGEPLPASWRAFLEQSDTKIDLSGCPVAAAATPTRSNAGDATKVTL ARTWYQALWATLCQPLLQTLVPYSVLGLVIFLPLRGLLAVAAATRFPLYWLLPAFWAA SGVAAMATCAAAKWALVGSRVDGDTVHIWSPAVFLDTVWQAVRAATAEYFAELTPGSA PFAAWMRVMGASVSPGDGVYVDSMGALLNPEMVRLERGAAVGRDALLFGHVYEGEAGK VKFGAVSVGEDGFVGSRAVAMPSVTVDDGGCLAALGLAMKGETVKHSM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;7294..9882#3309..6578#3033..3211#2593..2952#2066..2507#1820..1953#1470..1673#1..235#atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggaggtatggattccccttccccactcgctgcgtgatgaaccgatgaaccgtagtacttgatgatatgcctgatttgcttcggcattgtgccgcttctctgttagctgggagtttgtggcgtgtcgtttaactgaagtagagatgtgatgatgatctgaggtgagcgccattttttttttttggctaactctgtttgttctattcagtgaatatgagctctcttttttgtcccctttttatcattctgcttctatgggtcgcggagcgttcttggttggtgcagtgcaactgcaatgtcagtatgcatgccatagtaatgtgatggtgggatgtaatgctcctctgcttgctcgagctctttcttggttgtctatctatcttggatgatttgatcaatgtactacttattatctgctgatagtacccggggtccatttgcgctagtattgaacttcttcttctcttataatttgctgaaatcgcgagattatcgcttaggtttatcaatcagaacattatcatgtttatgcttcggttcgttcggtactggcacatgtcaaataagcggaaattctgctactttttcggcttaccatgaatggcacccaacatagcgtggttgttttgtcatgttgaactagtacaaatttttgcaccttaatatttgctttccgtgcatatccaattaacctgcccatcaaacttcttttcagatagctattattggggaaaaatattttcccagcttcttttatagtgactagactgtttatacatgcctgcgtaattgcttccttttgatgagctagtacattatagaccttgattttgttttgatggtacgggttggttattggtgtccatataatgaaattgcaacttgcgaccactccatgatagttacctggtcattccttgttggttaataagctattatttcaaatatgaatgttcatcctacttgtcatattatagacatttgtcacaattcaagtgaggaagaaattctgtaattttctatgggctagttgctgcgttaactacaataatggggctgacatcctgaggttatagtgttcgattaccaagttctaatttattaagaggaatgcaagtcaacatgcatgcctagcccatttacttttaatgagtagtgtaacttctgttgcagaagtactaatgacagctttctatataaatttcacaggttgatttatcaaggacaacaattcgtcagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaaggtgaggaaactactcagcaaaattatacagtcatcattttgtgcacacttttagttatcagcaacattcgtatgtaggacgaatatatgatttgggaggacaagttatagcagcaaacagtgcccctgttataactcaccttgcagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggtgagttgttctagaataaaatttaaatttgaggtaactggataataagcatctaattatttataatattctctctatttcttgcatgcgtgttattcaataattcttacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactggtgagcatattttaaattcagtttggctggcattgaagctatagtatttcagttgccttattagtatgtatgatcttttgttcagatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaaggtatatatgttaataatatttctcctgttgcaaggtcttcttttttctcgtgataacattggtaatttcctgcattgcagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaaggtgagcaagaaataatggcgtaatatatctcaattattaattccaccttcatcaaatttcttagagaaattgtgcatatatttttccatacttgcagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagagg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ica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001187049.1 RefSeq:Os04g0473900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 4]]<br /> [[Category:Chromosome 4]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os04g0473900&diff=175253 2014-06-01T01:48:31Z

<p>Mayamei0424: /* Evolution */</p> <hr /> <div>''DWA1'' encodes an enzymatic megaprotein conserved in vascular plants，and ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''DWA1'' encodes an enzymatic megaprotein (2,391 aa in length) conserved in vascular plants, including an oxidoreductase-like domain, a prokaryotic nonribosomal peptide synthetase-like module, an AMP-binding domain, and an allene oxide synthase-like domain. The AMP-binding domain exhibits in vitro enzymatic activity in activating long-chain fatty acids to form acyl-CoA. Expression pattern analysis shows that ''DWA1'' is preferentially expressed in vascular tissues and epidermal layers and strongly induced by drought stress. The ''dwa1'' mutant was highly sensitive to drought stress compared to the WT and DWA1-functional complementation （DWA1-FC）plants restored drought resistance. Further dissections uncovers that the ''dwa1'' mutant was impaired in cuticular wax accumulation and significantly suppressed many wax-related genes expression under drought conditions and reduced levels of very-long-chain fatty acids. Conversely, plants overexpressing ''DWA1'' were significantly up-regulated many wax-related genes expression under drought conditions and elevated levels of very-long-chain fatty acids. The results suggest that ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice&lt;ref name=&quot;ref1&quot;/&gt;.<br /> <br /> ===Mutation===<br /> [[File:dwa1.jpg|right|thumb|200px|Fig.1 Screening of mutants(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa2.jpg|right|thumb|200px|Fig.2 phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa3.jpg|right|thumb|200px|Fig.3 functional complementation assay analysis(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa4.jpg|right|thumb|200px|Fig.4 Overexpression of ''DWA1'' analysis(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> '''(1)''' '''Screening of mutants(Identification of the drought-hypersensitive ''dwa1'' mutant in rice.)'''&lt;br&gt;<br /> ''dwa1'' mutant is observed by inserting T-DNA in the sixth exon of ''DWA1''(Fig.1A and B).RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1C and D).&lt;br&gt;<br /> '''(2)''' '''phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT'''&lt;br&gt;<br /> During the course of drought treatment, the mutant wilted and exhibited leaf rolling earlier than the WT. After severe drought treatment followed by rewatering, more than 90% of the WT plants survive, whereas the ''dwa1'' mutant plants are almost dead(Fig.2A and C). Water loss rate of mutant is faster than WT(Fig.2B).Furthermore, SEM analysis indicate that the deposition of vertical plate-like wax crystals on the ''dwa1'' leaf cuticle is severely reduced under normal and drought conditions in contrast to WT leaf(Fig.2D). The contents of individual FA components is obvious difference between the ''dwa1'' mutant and WT. Among them, VLCFAs are significantly reduced, whereas the levels of are slightly higher in the ''dwa1'' mutant(Fig.2E). The gene expression levels of wax-related genes are obviously different under drought treatment(Fig.2F)&lt;br&gt;<br /> '''(3) functional complementation assay analysis'''&lt;br&gt;<br /> DWA1-functional complementation(DWA1-FC)lines which is inserted the full-length genomic fragment of ''DWA1'' into the ''dwa1'' mutant restore drought resistance(Fig.3).&lt;br&gt;<br /> '''(4)''' '''Overexpression of ''DWA1'' analysis'''&lt;br&gt;<br /> There are three independent overexpression plants(U7,U9 and U10) and a negative control (U4),and there is no visual differences in the epicuticular wax load compared with the control (Fig. 4B).But the VLCFA constituents increase in the overexpression lines compared with WT. However, the contents of LCFAs are significantly reduced in the overexpression plants. The alkanes and primary alcohols showed no significant changes (Fig. 4C). Under normal growth conditions, the expression levels of wax-related genes enhance in the overexpression plants (Fig.4D).<br /> <br /> ===Expression===<br /> [[File:图片4.png|right|thumb|200px|Fig.5 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:图片5.png|right|thumb|200px|Fig.6 Drought-induced expression of ''DWA1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> '''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.5A).&lt;br&gt;<br /> '''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.5B1 and 2), axillary bud (Fig.5B3), young panicle (Fig.5B4),spikelet (Fig.5B5), stem (Fig.5B6), young panicle (Fig.5B7), pistil (Fig.5B8), stamen (Fig.5B9), young embryo (Fig.5B10), SEM picture of rice leaf cuticle (Fig.5B11), cross-sections of stem (Fig.5B12) and leaf (Fig.5B13),and semithin sections of leaf (Fig.5B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.&lt;br&gt;<br /> '''(3)'''What's more,after various phytohormones treatment,''DWA1'' expresses higher,such as abscisic acid, jasmonic acid, indole-3-acetic acid, and<br /> gibberellic acid (Fig.5C).&lt;br&gt;<br /> '''(4)'''Under drought treatment,GUS staining shows that''DWA1'' is induced.For example,in Fig.6B,GUS staining in leaf before (1 and 3) and after (2 and 4) drought stress is shown.&lt;br&gt;<br /> <br /> ===Evolution===<br /> [[File:1(DWA1).png|center|thumb|200px|Fig.7 Unrooted phylogenetic tree and domain architecture of DWA1 protein orthologs in vascular plants and microbial relatives(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region&lt;ref name=&quot;ref1&quot;/&gt;(Fig.7).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi&lt;ref name=&quot;ref2&quot;/&gt;,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain&lt;ref name=&quot;ref1&quot;/&gt;.<br /> <br /> ==Labs working on this gene==<br /> *National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan,China<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Xiaoyi Zhu, Lizhong Xiong.（2013）Putative megaenzyme DWA1 plays essential roles in drought resistance by regulating stress-induced wax<br /> deposition in rice.PANS 110(44): 17790-17795.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Marahiel MA, Stachelhaus T, Mootz HD.(1997) Modular peptide synthetases involved in nonribosomal peptide synthesis.Chem Rev 97(7):2651–2674.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os04g0473900|<br /> Description = Adrenodoxin reductase family protein|<br /> Version = NM_001187049.1 GI:297723228 GeneID:9266980|<br /> Length = 9882 bp|<br /> Definition = Oryza sativa Japonica Group Os04g0473900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|<br /> AP = Chromosome 4:24097480..24107361|<br /> CDS = 24097480..24100068,24100784..24104053,24104151..24104329,24104410..24104769,24104855..24105296&lt;br&gt;,24105409..24105542,24105689..24105892,24107127..24107361|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008397:24097480..24107361<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:24097480..24107361<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactgatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttctggctcagaaaggcatctcaattccagattcttctgatcaggttggacttgttgtgattgcagaggttagagagggtaaggcagtctctaaagaggttgttaacaacatcaaagcacgtgtggtggaggagcatggtgtagctgttgcatctgtgaagcttatcaagcctagaaccatatgcaagacaacatctgggaagatacgaagatttgaatgcatgaggcagtttgttgacaatactcttagtttggctaagggcaaccatgtatctaagaaaaagggtctttttcggtctctgacaacaggaactggcatggagtcaaagaggtccctcttgaggcaaactgttgacctcaccattagccattggcccaaaagccaagttaaaaactccaatgaaataactgagtttctgacacaaatagtgtcagagcacactggcatttcaaaggataaaatttctctaactgacagtctaccttcgtatggttttgattctattgcagtggtacgagcagcccagaagctctcagattttcttggtgttcctgttggggccattgacatctttacagcaagctgcatttcggagcttgcaagcttcttggagaatttggtgcataagtcacaaacacagttggcaccttggcccaaaagcaaagtgaaaaactccaaggtgattatcgagtttctgacaaagattgtatcagatcaaacagggattccaaaggataaaatttctccgatggatagcctaccttcatatggttttgattcaattgcggtggtccaagcagcacagaaactctccgattttctcggtgtacctgttggagccattgacatatttacagctggttgcatttctgagctagctaccttcttggagaatttggcgcataagtcacagtcacagttggcacctggtgcatcttgctatattgaggatgagactcaagtggatcccatggatgccattagtccagaattcagtgttttgggtacgggcatcctgcaactgctggctcttacttatgtctgttttgtgctgctactcccagcatatcttgctagttcgacatacatgagcattttctctacagtcagtctggtcagatcaccattgctgagctatcttagttctttggttatggcacctattgtgtggatcttttatatctcgctcacttctttatcactgtctatcttggggaagtccttcctacagccaaattatgttctgatccctgatgtttcaatatggtcagttgactttgtgaaatggtgggcactaaacaaagcacaagcattagctgcaaagatgctagctgtgcatctaaagggcacaattttcttaaattattggttcaagatgcaaggagctcggattggttcatctgttgtcatcgacacagtagacataaccgatccatccctgttgacggttgcagatggtgcagttcttgcagaaggcgctcttgttcagggccatgaagtctgtaatgaagtgctgagttttaggcctatctggattggctgtgaggcttcgattggtccttatgcggtgcttcagaagggaactgttgtggaagatggagctgttgttccaccattgcagaagactggggctgggaaatcaacacgtcggactagcagaacttctgtaagcataaagaaagaagcagccaaagcaaacatgatacttgaacacctcgttagcatctacgcagttggcatcctgggtgctctgtccggtgcaatcgtctacacactatacactcacttgtccggcaaagcggcttcgcctctgcatttctcctttgcatgcattgctggtgcgttccactggttaccagctgccatcactgcctatgctgttattgtccaggagacacccacaagtgcactgtcatttgctctcttcactgccttcgccgacctgagctatggtgtcatcctcagtattctcaccagtataaccagcagagcccttgctgcaaagcctggaaccaagcagaatggcattgcgagtttgatccaccggcgcataaccatctctgctcatgtcaggtttgccaagatgctatctggaacagaagccttctgcgtgtacctgaggcttctcggagcaaagattggcaggcattgctctatccgagccatcaatccggtggcgaatcctgagctgatcagcgtcggtgatggcgtccatctaggtgacttctgcaatattgttccagggttctactccaaggggggattcaccagtgcagagattaaggtccaggagaacaccgtggttggcagtggcagcttgctcctccctggctgtgtcctccaggagaatgtcatcctcggcgcgctctccgtcgcgccggagaacgctgttctccggcgtggcggcgtctacgtcgggtcacagtctccggccatggtgaagaacaccttgcttgacgaggatgaacggatcgaagagatggatcaggcatacaagaagattgttggcaacctcgcggcgaacttggccatcaccaccatgaacgtcaagtcgaggtacttccaccgcatcggcgtgagcggccgcggcgtcctgaggatgtacgaggagataccgtcgtttccccggcacaagatcttcgcctccggcaagtccttcccggtgatcgtccgccacagcaacagcctgagcgccgacgacgacgcgaggctcgacgcccgcggcgccgcggtgcggatcctctcggacaacgacggcgaggcgcctctcctcgacctcacactcaagagcggcaaggcgttctacgcgcgcaccatcgccgacttcgccacctggctggtctgcggcctgccggcgagggaggagcaggtcaagcgctcgccgcacatccgcgacgccgtctggggctctctccggagcaccgactcgtacaccgtgctccactactactccaacatatgccggctgctgcggttcgacgacggcagggagatgtacgccaagttcaagctccgtcctgctgatccagacgtccccgaggactccggcaaggtggtgccgaggggcatcctgccgccggagaccggcgcgatccccagggacgaggatgacacgcgccctctgctcttcctcgccgacgacttccgccggagagtgggctcaccggacggggtgcgctacgtgttccagctgcagctcagggaggtgcccaccgacgccgccgcccgcgacgtcgccctcgactgcacccggccgtgggacgaggccgagttcccttacatcgacgtcggcgaggtcagcatcggccgcaacctcccgacggaggagacggagaagctcgagttcaacccgttcctccggtgccccgaggtggacgtcatcccggcgacgtcgtgcgcgcagagcgcgtccatcgaccacggccgctcgctggtctacgagatatgccagcgcctgcggaacggcgagccgctgcccgcgtcgtggcgagctttcctggagcagtcggacaccaagatcgacctgtcaggatgcccggtcgctgccgccgcgacacccacaaggtctaacgccggcgacgccacgaaggtgacgctggccaggacgtggtaccaggcgctctgggccacgctgtgccagccgctgctgcaaacgctggtgccgtactctgtcctgggcctcgtcatcttcctcccgctcaggggcctcctcgccgtcgccgcggccacccgcttcccgctctactggctcctcccggcgttctgggccgcgtcgggcgtcgccgccatggccacgtgcgcggcggcgaagtgggcgctggtcggcagcagggtcgacggcgacacggtgcacatctggtcgccggccgtgttcctggacacggtctggcaggccgtgcgcgccgccacggcggagtacttcgccgagctgacgcccgggtcggcgccgttcgcggcgtggatgcgggtgatgggcgcctccgtgtcccccggcgacggcgtgtacgtggactccatgggcgcgctgctgaacccggagatggtgcgcctggagcgcggcgccgccgtcgggcgggacgcgctgctgttcggccacgtgtacgagggcgaggccgggaaggtgaagttcggcgccgtgtccgtcggggaggacgggttcgtcggcagccgcgccgtggccatgcccagcgtcacggtggacgacggcggctgcctcgccgcgctcgggctggccatgaagggggagacggtgaagcacagcatgtaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MEERRATSPSRHSFCRDALGRRGGESGGGLVYIYRSPLRSPLQR HGLNREWRLERWRWRWRWEAATWRQRAAAHARIGEMDPKRSIDDQFSKLHPSLPVDTR IGIVGAGPSGLSAAYALAKLGYRNVTLFEKCHTVSGMCESIDIEEELGSDFEEMDTHK LSLIDSQTGNIRDLEVAEDYVSMVSLTLKLQDEANKSGRAGLHALSGLASDPTHEFLK QNGINSMPKSVAYGYTASGYGFVQDMPYAFIQEFTRTSMAGKIRRFKHGYMSMWERLS KSLPFEVFCDTQVLNVKRNSCGANVTIKNNNGEKQVLEFDKIILSGAVAFKNSKTYRS SSLTDGESEVVELNNLERELFSKVQTIDYYTTVVKINGFEHIPKGFYYFGEFMEDPTT IGHPVAMQRFFADTNIFLFWSYGNSADIKGSFVAKCVTDVVSSMGGNVQKVILQRRFK YFPHVSSKDMKDGFYERLESQLQGFQNTYYVGGLLAFELTERNASYSIMAVCKHFAID GEGELTPYVKRLFPLSHNRNPSPPRDLGELEGVEFPDLPSLDGYLQYWGTHKVTEKNV IYTWINEEGKLMNRRTYQELHGNASYIAQKLLTSTKPVIKPGDRVLLIHLPGLEFIDA FFGCIRAGVIPVPVLPPDPMQSGGQALLKVENISKMCNAVAILSTSSYHAAVRAGYIK NIVTLAKRVQKCSAQWPDIPWIHTDSWIKNYRRSSDSFNSDTVLFTKPQPSDLCFLQF TSGSTGDAKGVMITHEGLIHNVKTMKKRYRSTSKTVLVSWLPQYHDMGLIGGLFTALV SGGTSVLFSPMIFIRNPLLWLQTINDYHGTHSAGPNFAFELVIRRLEAEKNKVYDLSS MVFLMIAAEPVRQKTVRRFIELTQPFGLSEGVLAPGYGLAENCVYVTCAFGECKPVFI DWQGRVCCGYVEQDDTDTLIRIVDPDSLTEHQEDGVEGEIWISSPSSGVGYWGNSEMS QRTFFNQLKNHPNKKFTRTGDLGRTIDGNLFITGRIKDLIIVAGRNIYSADVEKTVES SSEVLRPGCCAVVGIPEEVLAQKGISIPDSSDQVGLVVIAEVREGKAVSKEVVNNIKA RVVEEHGVAVASVKLIKPRTICKTTSGKIRRFECMRQFVDNTLSLAKGNHVSKKKGLF RSLTTGTGMESKRSLLRQTVDLTISHWPKSQVKNSNEITEFLTQIVSEHTGISKDKIS LTDSLPSYGFDSIAVVRAAQKLSDFLGVPVGAIDIFTASCISELASFLENLVHKSQTQ LAPWPKSKVKNSKVIIEFLTKIVSDQTGIPKDKISPMDSLPSYGFDSIAVVQAAQKLS DFLGVPVGAIDIFTAGCISELATFLENLAHKSQSQLAPGASCYIEDETQVDPMDAISP EFSVLGTGILQLLALTYVCFVLLLPAYLASSTYMSIFSTVSLVRSPLLSYLSSLVMAP IVWIFYISLTSLSLSILGKSFLQPNYVLIPDVSIWSVDFVKWWALNKAQALAAKMLAV HLKGTIFLNYWFKMQGARIGSSVVIDTVDITDPSLLTVADGAVLAEGALVQGHEVCNE VLSFRPIWIGCEASIGPYAVLQKGTVVEDGAVVPPLQKTGAGKSTRRTSRTSVSIKKE AAKANMILEHLVSIYAVGILGALSGAIVYTLYTHLSGKAASPLHFSFACIAGAFHWLP AAITAYAVIVQETPTSALSFALFTAFADLSYGVILSILTSITSRALAAKPGTKQNGIA SLIHRRITISAHVRFAKMLSGTEAFCVYLRLLGAKIGRHCSIRAINPVANPELISVGD GVHLGDFCNIVPGFYSKGGFTSAEIKVQENTVVGSGSLLLPGCVLQENVILGALSVAP ENAVLRRGGVYVGSQSPAMVKNTLLDEDERIEEMDQAYKKIVGNLAANLAITTMNVKS RYFHRIGVSGRGVLRMYEEIPSFPRHKIFASGKSFPVIVRHSNSLSADDDARLDARGA AVRILSDNDGEAPLLDLTLKSGKAFYARTIADFATWLVCGLPAREEQVKRSPHIRDAV WGSLRSTDSYTVLHYYSNICRLLRFDDGREMYAKFKLRPADPDVPEDSGKVVPRGILP PETGAIPRDEDDTRPLLFLADDFRRRVGSPDGVRYVFQLQLREVPTDAAARDVALDCT RPWDEAEFPYIDVGEVSIGRNLPTEETEKLEFNPFLRCPEVDVIPATSCAQSASIDHG RSLVYEICQRLRNGEPLPASWRAFLEQSDTKIDLSGCPVAAAATPTRSNAGDATKVTL ARTWYQALWATLCQPLLQTLVPYSVLGLVIFLPLRGLLAVAAATRFPLYWLLPAFWAA SGVAAMATCAAAKWALVGSRVDGDTVHIWSPAVFLDTVWQAVRAATAEYFAELTPGSA PFAAWMRVMGASVSPGDGVYVDSMGALLNPEMVRLERGAAVGRDALLFGHVYEGEAGK VKFGAVSVGEDGFVGSRAVAMPSVTVDDGGCLAALGLAMKGETVKHSM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;7294..9882#3309..6578#3033..3211#2593..2952#2066..2507#1820..1953#1470..1673#1..235#atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggaggtatggattccccttccccactcgctgcgtgatgaaccgatgaaccgtagtacttgatgatatgcctgatttgcttcggcattgtgccgcttctctgttagctgggagtttgtggcgtgtcgtttaactgaagtagagatgtgatgatgatctgaggtgagcgccattttttttttttggctaactctgtttgttctattcagtgaatatgagctctcttttttgtcccctttttatcattctgcttctatgggtcgcggagcgttcttggttggtgcagtgcaactgcaatgtcagtatgcatgccatagtaatgtgatggtgggatgtaatgctcctctgcttgctcgagctctttcttggttgtctatctatcttggatgatttgatcaatgtactacttattatctgctgatagtacccggggtccatttgcgctagtattgaacttcttcttctcttataatttgctgaaatcgcgagattatcgcttaggtttatcaatcagaacattatcatgtttatgcttcggttcgttcggtactggcacatgtcaaataagcggaaattctgctactttttcggcttaccatgaatggcacccaacatagcgtggttgttttgtcatgttgaactagtacaaatttttgcaccttaatatttgctttccgtgcatatccaattaacctgcccatcaaacttcttttcagatagctattattggggaaaaatattttcccagcttcttttatagtgactagactgtttatacatgcctgcgtaattgcttccttttgatgagctagtacattatagaccttgattttgttttgatggtacgggttggttattggtgtccatataatgaaattgcaacttgcgaccactccatgatagttacctggtcattccttgttggttaataagctattatttcaaatatgaatgttcatcctacttgtcatattatagacatttgtcacaattcaagtgaggaagaaattctgtaattttctatgggctagttgctgcgttaactacaataatggggctgacatcctgaggttatagtgttcgattaccaagttctaatttattaagaggaatgcaagtcaacatgcatgcctagcccatttacttttaatgagtagtgtaacttctgttgcagaagtactaatgacagctttctatataaatttcacaggttgatttatcaaggacaacaattcgtcagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaaggtgaggaaactactcagcaaaattatacagtcatcattttgtgcacacttttagttatcagcaacattcgtatgtaggacgaatatatgatttgggaggacaagttatagcagcaaacagtgcccctgttataactcaccttgcagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggtgagttgttctagaataaaatttaaatttgaggtaactggataataagcatctaattatttataatattctctctatttcttgcatgcgtgttattcaataattcttacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactggtgagcatattttaaattcagtttggctggcattgaagctatagtatttcagttgccttattagtatgtatgatcttttgttcagatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaaggtatatatgttaataatatttctcctgttgcaaggtcttcttttttctcgtgataacattggtaatttcctgcattgcagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaaggtgagcaagaaataatggcgtaatatatctcaattattaattccaccttcatcaaatttcttagagaaattgtgcatatatttttccatacttgcagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagagg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ica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001187049.1 RefSeq:Os04g0473900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 4]]<br /> [[Category:Chromosome 4]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os04g0473900&diff=175252 2014-06-01T01:47:47Z

<p>Mayamei0424: /* Expression */</p> <hr /> <div>''DWA1'' encodes an enzymatic megaprotein conserved in vascular plants，and ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''DWA1'' encodes an enzymatic megaprotein (2,391 aa in length) conserved in vascular plants, including an oxidoreductase-like domain, a prokaryotic nonribosomal peptide synthetase-like module, an AMP-binding domain, and an allene oxide synthase-like domain. The AMP-binding domain exhibits in vitro enzymatic activity in activating long-chain fatty acids to form acyl-CoA. Expression pattern analysis shows that ''DWA1'' is preferentially expressed in vascular tissues and epidermal layers and strongly induced by drought stress. The ''dwa1'' mutant was highly sensitive to drought stress compared to the WT and DWA1-functional complementation （DWA1-FC）plants restored drought resistance. Further dissections uncovers that the ''dwa1'' mutant was impaired in cuticular wax accumulation and significantly suppressed many wax-related genes expression under drought conditions and reduced levels of very-long-chain fatty acids. Conversely, plants overexpressing ''DWA1'' were significantly up-regulated many wax-related genes expression under drought conditions and elevated levels of very-long-chain fatty acids. The results suggest that ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice&lt;ref name=&quot;ref1&quot;/&gt;.<br /> <br /> ===Mutation===<br /> [[File:dwa1.jpg|right|thumb|200px|Fig.1 Screening of mutants(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa2.jpg|right|thumb|200px|Fig.2 phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa3.jpg|right|thumb|200px|Fig.3 functional complementation assay analysis(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa4.jpg|right|thumb|200px|Fig.4 Overexpression of ''DWA1'' analysis(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> '''(1)''' '''Screening of mutants(Identification of the drought-hypersensitive ''dwa1'' mutant in rice.)'''&lt;br&gt;<br /> ''dwa1'' mutant is observed by inserting T-DNA in the sixth exon of ''DWA1''(Fig.1A and B).RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1C and D).&lt;br&gt;<br /> '''(2)''' '''phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT'''&lt;br&gt;<br /> During the course of drought treatment, the mutant wilted and exhibited leaf rolling earlier than the WT. After severe drought treatment followed by rewatering, more than 90% of the WT plants survive, whereas the ''dwa1'' mutant plants are almost dead(Fig.2A and C). Water loss rate of mutant is faster than WT(Fig.2B).Furthermore, SEM analysis indicate that the deposition of vertical plate-like wax crystals on the ''dwa1'' leaf cuticle is severely reduced under normal and drought conditions in contrast to WT leaf(Fig.2D). The contents of individual FA components is obvious difference between the ''dwa1'' mutant and WT. Among them, VLCFAs are significantly reduced, whereas the levels of are slightly higher in the ''dwa1'' mutant(Fig.2E). The gene expression levels of wax-related genes are obviously different under drought treatment(Fig.2F)&lt;br&gt;<br /> '''(3) functional complementation assay analysis'''&lt;br&gt;<br /> DWA1-functional complementation(DWA1-FC)lines which is inserted the full-length genomic fragment of ''DWA1'' into the ''dwa1'' mutant restore drought resistance(Fig.3).&lt;br&gt;<br /> '''(4)''' '''Overexpression of ''DWA1'' analysis'''&lt;br&gt;<br /> There are three independent overexpression plants(U7,U9 and U10) and a negative control (U4),and there is no visual differences in the epicuticular wax load compared with the control (Fig. 4B).But the VLCFA constituents increase in the overexpression lines compared with WT. However, the contents of LCFAs are significantly reduced in the overexpression plants. The alkanes and primary alcohols showed no significant changes (Fig. 4C). Under normal growth conditions, the expression levels of wax-related genes enhance in the overexpression plants (Fig.4D).<br /> <br /> ===Expression===<br /> [[File:图片4.png|right|thumb|200px|Fig.5 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:图片5.png|right|thumb|200px|Fig.6 Drought-induced expression of ''DWA1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> '''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.5A).&lt;br&gt;<br /> '''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.5B1 and 2), axillary bud (Fig.5B3), young panicle (Fig.5B4),spikelet (Fig.5B5), stem (Fig.5B6), young panicle (Fig.5B7), pistil (Fig.5B8), stamen (Fig.5B9), young embryo (Fig.5B10), SEM picture of rice leaf cuticle (Fig.5B11), cross-sections of stem (Fig.5B12) and leaf (Fig.5B13),and semithin sections of leaf (Fig.5B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.&lt;br&gt;<br /> '''(3)'''What's more,after various phytohormones treatment,''DWA1'' expresses higher,such as abscisic acid, jasmonic acid, indole-3-acetic acid, and<br /> gibberellic acid (Fig.5C).&lt;br&gt;<br /> '''(4)'''Under drought treatment,GUS staining shows that''DWA1'' is induced.For example,in Fig.6B,GUS staining in leaf before (1 and 3) and after (2 and 4) drought stress is shown.&lt;br&gt;<br /> <br /> ===Evolution===<br /> [[File:1(DWA1).png|right|thumb|200px|Fig.4 Unrooted phylogenetic tree and domain architecture of DWA1 protein orthologs in vascular plants and microbial relatives(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region&lt;ref name=&quot;ref1&quot;/&gt;(Fig.4).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi&lt;ref name=&quot;ref2&quot;/&gt;,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain&lt;ref name=&quot;ref1&quot;/&gt;.<br /> <br /> ==Labs working on this gene==<br /> *National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan,China<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Xiaoyi Zhu, Lizhong Xiong.（2013）Putative megaenzyme DWA1 plays essential roles in drought resistance by regulating stress-induced wax<br /> deposition in rice.PANS 110(44): 17790-17795.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Marahiel MA, Stachelhaus T, Mootz HD.(1997) Modular peptide synthetases involved in nonribosomal peptide synthesis.Chem Rev 97(7):2651–2674.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os04g0473900|<br /> Description = Adrenodoxin reductase family protein|<br /> Version = NM_001187049.1 GI:297723228 GeneID:9266980|<br /> Length = 9882 bp|<br /> Definition = Oryza sativa Japonica Group Os04g0473900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|<br /> AP = Chromosome 4:24097480..24107361|<br /> CDS = 24097480..24100068,24100784..24104053,24104151..24104329,24104410..24104769,24104855..24105296&lt;br&gt;,24105409..24105542,24105689..24105892,24107127..24107361|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008397:24097480..24107361<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:24097480..24107361<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactgatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttctggctcagaaaggcatctcaattccagattcttctgatcaggttggacttgttgtgattgcagaggttagagagggtaaggcagtctctaaagaggttgttaacaacatcaaagcacgtgtggtggaggagcatggtgtagctgttgcatctgtgaagcttatcaagcctagaaccatatgcaagacaacatctgggaagatacgaagatttgaatgcatgaggcagtttgttgacaatactcttagtttggctaagggcaaccatgtatctaagaaaaagggtctttttcggtctctgacaacaggaactggcatggagtcaaagaggtccctcttgaggcaaactgttgacctcaccattagccattggcccaaaagccaagttaaaaactccaatgaaataactgagtttctgacacaaatagtgtcagagcacactggcatttcaaaggataaaatttctctaactgacagtctaccttcgtatggttttgattctattgcagtggtacgagcagcccagaagctctcagattttcttggtgttcctgttggggccattgacatctttacagcaagctgcatttcggagcttgcaagcttcttggagaatttggtgcataagtcacaaacacagttggcaccttggcccaaaagcaaagtgaaaaactccaaggtgattatcgagtttctgacaaagattgtatcagatcaaacagggattccaaaggataaaatttctccgatggatagcctaccttcatatggttttgattcaattgcggtggtccaagcagcacagaaactctccgattttctcggtgtacctgttggagccattgacatatttacagctggttgcatttctgagctagctaccttcttggagaatttggcgcataagtcacagtcacagttggcacctggtgcatcttgctatattgaggatgagactcaagtggatcccatggatgccattagtccagaattcagtgttttgggtacgggcatcctgcaactgctggctcttacttatgtctgttttgtgctgctactcccagcatatcttgctagttcgacatacatgagcattttctctacagtcagtctggtcagatcaccattgctgagctatcttagttctttggttatggcacctattgtgtggatcttttatatctcgctcacttctttatcactgtctatcttggggaagtccttcctacagccaaattatgttctgatccctgatgtttcaatatggtcagttgactttgtgaaatggtgggcactaaacaaagcacaagcattagctgcaaagatgctagctgtgcatctaaagggcacaattttcttaaattattggttcaagatgcaaggagctcggattggttcatctgttgtcatcgacacagtagacataaccgatccatccctgttgacggttgcagatggtgcagttcttgcagaaggcgctcttgttcagggccatgaagtctgtaatgaagtgctgagttttaggcctatctggattggctgtgaggcttcgattggtccttatgcggtgcttcagaagggaactgttgtggaagatggagctgttgttccaccattgcagaagactggggctgggaaatcaacacgtcggactagcagaacttctgtaagcataaagaaagaagcagccaaagcaaacatgatacttgaacacctcgttagcatctacgcagttggcatcctgggtgctctgtccggtgcaatcgtctacacactatacactcacttgtccggcaaagcggcttcgcctctgcatttctcctttgcatgcattgctggtgcgttccactggttaccagctgccatcactgcctatgctgttattgtccaggagacacccacaagtgcactgtcatttgctctcttcactgccttcgccgacctgagctatggtgtcatcctcagtattctcaccagtataaccagcagagcccttgctgcaaagcctggaaccaagcagaatggcattgcgagtttgatccaccggcgcataaccatctctgctcatgtcaggtttgccaagatgctatctggaacagaagccttctgcgtgtacctgaggcttctcggagcaaagattggcaggcattgctctatccgagccatcaatccggtggcgaatcctgagctgatcagcgtcggtgatggcgtccatctaggtgacttctgcaatattgttccagggttctactccaaggggggattcaccagtgcagagattaaggtccaggagaacaccgtggttggcagtggcagcttgctcctccctggctgtgtcctccaggagaatgtcatcctcggcgcgctctccgtcgcgccggagaacgctgttctccggcgtggcggcgtctacgtcgggtcacagtctccggccatggtgaagaacaccttgcttgacgaggatgaacggatcgaagagatggatcaggcatacaagaagattgttggcaacctcgcggcgaacttggccatcaccaccatgaacgtcaagtcgaggtacttccaccgcatcggcgtgagcggccgcggcgtcctgaggatgtacgaggagataccgtcgtttccccggcacaagatcttcgcctccggcaagtccttcccggtgatcgtccgccacagcaacagcctgagcgccgacgacgacgcgaggctcgacgcccgcggcgccgcggtgcggatcctctcggacaacgacggcgaggcgcctctcctcgacctcacactcaagagcggcaaggcgttctacgcgcgcaccatcgccgacttcgccacctggctggtctgcggcctgccggcgagggaggagcaggtcaagcgctcgccgcacatccgcgacgccgtctggggctctctccggagcaccgactcgtacaccgtgctccactactactccaacatatgccggctgctgcggttcgacgacggcagggagatgtacgccaagttcaagctccgtcctgctgatccagacgtccccgaggactccggcaaggtggtgccgaggggcatcctgccgccggagaccggcgcgatccccagggacgaggatgacacgcgccctctgctcttcctcgccgacgacttccgccggagagtgggctcaccggacggggtgcgctacgtgttccagctgcagctcagggaggtgcccaccgacgccgccgcccgcgacgtcgccctcgactgcacccggccgtgggacgaggccgagttcccttacatcgacgtcggcgaggtcagcatcggccgcaacctcccgacggaggagacggagaagctcgagttcaacccgttcctccggtgccccgaggtggacgtcatcccggcgacgtcgtgcgcgcagagcgcgtccatcgaccacggccgctcgctggtctacgagatatgccagcgcctgcggaacggcgagccgctgcccgcgtcgtggcgagctttcctggagcagtcggacaccaagatcgacctgtcaggatgcccggtcgctgccgccgcgacacccacaaggtctaacgccggcgacgccacgaaggtgacgctggccaggacgtggtaccaggcgctctgggccacgctgtgccagccgctgctgcaaacgctggtgccgtactctgtcctgggcctcgtcatcttcctcccgctcaggggcctcctcgccgtcgccgcggccacccgcttcccgctctactggctcctcccggcgttctgggccgcgtcgggcgtcgccgccatggccacgtgcgcggcggcgaagtgggcgctggtcggcagcagggtcgacggcgacacggtgcacatctggtcgccggccgtgttcctggacacggtctggcaggccgtgcgcgccgccacggcggagtacttcgccgagctgacgcccgggtcggcgccgttcgcggcgtggatgcgggtgatgggcgcctccgtgtcccccggcgacggcgtgtacgtggactccatgggcgcgctgctgaacccggagatggtgcgcctggagcgcggcgccgccgtcgggcgggacgcgctgctgttcggccacgtgtacgagggcgaggccgggaaggtgaagttcggcgccgtgtccgtcggggaggacgggttcgtcggcagccgcgccgtggccatgcccagcgtcacggtggacgacggcggctgcctcgccgcgctcgggctggccatgaagggggagacggtgaagcacagcatgtaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MEERRATSPSRHSFCRDALGRRGGESGGGLVYIYRSPLRSPLQR HGLNREWRLERWRWRWRWEAATWRQRAAAHARIGEMDPKRSIDDQFSKLHPSLPVDTR IGIVGAGPSGLSAAYALAKLGYRNVTLFEKCHTVSGMCESIDIEEELGSDFEEMDTHK LSLIDSQTGNIRDLEVAEDYVSMVSLTLKLQDEANKSGRAGLHALSGLASDPTHEFLK QNGINSMPKSVAYGYTASGYGFVQDMPYAFIQEFTRTSMAGKIRRFKHGYMSMWERLS KSLPFEVFCDTQVLNVKRNSCGANVTIKNNNGEKQVLEFDKIILSGAVAFKNSKTYRS SSLTDGESEVVELNNLERELFSKVQTIDYYTTVVKINGFEHIPKGFYYFGEFMEDPTT IGHPVAMQRFFADTNIFLFWSYGNSADIKGSFVAKCVTDVVSSMGGNVQKVILQRRFK YFPHVSSKDMKDGFYERLESQLQGFQNTYYVGGLLAFELTERNASYSIMAVCKHFAID GEGELTPYVKRLFPLSHNRNPSPPRDLGELEGVEFPDLPSLDGYLQYWGTHKVTEKNV IYTWINEEGKLMNRRTYQELHGNASYIAQKLLTSTKPVIKPGDRVLLIHLPGLEFIDA FFGCIRAGVIPVPVLPPDPMQSGGQALLKVENISKMCNAVAILSTSSYHAAVRAGYIK NIVTLAKRVQKCSAQWPDIPWIHTDSWIKNYRRSSDSFNSDTVLFTKPQPSDLCFLQF TSGSTGDAKGVMITHEGLIHNVKTMKKRYRSTSKTVLVSWLPQYHDMGLIGGLFTALV SGGTSVLFSPMIFIRNPLLWLQTINDYHGTHSAGPNFAFELVIRRLEAEKNKVYDLSS MVFLMIAAEPVRQKTVRRFIELTQPFGLSEGVLAPGYGLAENCVYVTCAFGECKPVFI DWQGRVCCGYVEQDDTDTLIRIVDPDSLTEHQEDGVEGEIWISSPSSGVGYWGNSEMS QRTFFNQLKNHPNKKFTRTGDLGRTIDGNLFITGRIKDLIIVAGRNIYSADVEKTVES SSEVLRPGCCAVVGIPEEVLAQKGISIPDSSDQVGLVVIAEVREGKAVSKEVVNNIKA RVVEEHGVAVASVKLIKPRTICKTTSGKIRRFECMRQFVDNTLSLAKGNHVSKKKGLF RSLTTGTGMESKRSLLRQTVDLTISHWPKSQVKNSNEITEFLTQIVSEHTGISKDKIS LTDSLPSYGFDSIAVVRAAQKLSDFLGVPVGAIDIFTASCISELASFLENLVHKSQTQ LAPWPKSKVKNSKVIIEFLTKIVSDQTGIPKDKISPMDSLPSYGFDSIAVVQAAQKLS DFLGVPVGAIDIFTAGCISELATFLENLAHKSQSQLAPGASCYIEDETQVDPMDAISP EFSVLGTGILQLLALTYVCFVLLLPAYLASSTYMSIFSTVSLVRSPLLSYLSSLVMAP IVWIFYISLTSLSLSILGKSFLQPNYVLIPDVSIWSVDFVKWWALNKAQALAAKMLAV HLKGTIFLNYWFKMQGARIGSSVVIDTVDITDPSLLTVADGAVLAEGALVQGHEVCNE VLSFRPIWIGCEASIGPYAVLQKGTVVEDGAVVPPLQKTGAGKSTRRTSRTSVSIKKE AAKANMILEHLVSIYAVGILGALSGAIVYTLYTHLSGKAASPLHFSFACIAGAFHWLP AAITAYAVIVQETPTSALSFALFTAFADLSYGVILSILTSITSRALAAKPGTKQNGIA SLIHRRITISAHVRFAKMLSGTEAFCVYLRLLGAKIGRHCSIRAINPVANPELISVGD GVHLGDFCNIVPGFYSKGGFTSAEIKVQENTVVGSGSLLLPGCVLQENVILGALSVAP ENAVLRRGGVYVGSQSPAMVKNTLLDEDERIEEMDQAYKKIVGNLAANLAITTMNVKS RYFHRIGVSGRGVLRMYEEIPSFPRHKIFASGKSFPVIVRHSNSLSADDDARLDARGA AVRILSDNDGEAPLLDLTLKSGKAFYARTIADFATWLVCGLPAREEQVKRSPHIRDAV WGSLRSTDSYTVLHYYSNICRLLRFDDGREMYAKFKLRPADPDVPEDSGKVVPRGILP PETGAIPRDEDDTRPLLFLADDFRRRVGSPDGVRYVFQLQLREVPTDAAARDVALDCT RPWDEAEFPYIDVGEVSIGRNLPTEETEKLEFNPFLRCPEVDVIPATSCAQSASIDHG RSLVYEICQRLRNGEPLPASWRAFLEQSDTKIDLSGCPVAAAATPTRSNAGDATKVTL ARTWYQALWATLCQPLLQTLVPYSVLGLVIFLPLRGLLAVAAATRFPLYWLLPAFWAA SGVAAMATCAAAKWALVGSRVDGDTVHIWSPAVFLDTVWQAVRAATAEYFAELTPGSA PFAAWMRVMGASVSPGDGVYVDSMGALLNPEMVRLERGAAVGRDALLFGHVYEGEAGK VKFGAVSVGEDGFVGSRAVAMPSVTVDDGGCLAALGLAMKGETVKHSM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;7294..9882#3309..6578#3033..3211#2593..2952#2066..2507#1820..1953#1470..1673#1..235#atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggaggtatggattccccttccccactcgctgcgtgatgaaccgatgaaccgtagtacttgatgatatgcctgatttgcttcggcattgtgccgcttctctgttagctgggagtttgtggcgtgtcgtttaactgaagtagagatgtgatgatgatctgaggtgagcgccattttttttttttggctaactctgtttgttctattcagtgaatatgagctctcttttttgtcccctttttatcattctgcttctatgggtcgcggagcgttcttggttggtgcagtgcaactgcaatgtcagtatgcatgccatagtaatgtgatggtgggatgtaatgctcctctgcttgctcgagctctttcttggttgtctatctatcttggatgatttgatcaatgtactacttattatctgctgatagtacccggggtccatttgcgctagtattgaacttcttcttctcttataatttgctgaaatcgcgagattatcgcttaggtttatcaatcagaacattatcatgtttatgcttcggttcgttcggtactggcacatgtcaaataagcggaaattctgctactttttcggcttaccatgaatggcacccaacatagcgtggttgttttgtcatgttgaactagtacaaatttttgcaccttaatatttgctttccgtgcatatccaattaacctgcccatcaaacttcttttcagatagctattattggggaaaaatattttcccagcttcttttatagtgactagactgtttatacatgcctgcgtaattgcttccttttgatgagctagtacattatagaccttgattttgttttgatggtacgggttggttattggtgtccatataatgaaattgcaacttgcgaccactccatgatagttacctggtcattccttgttggttaataagctattatttcaaatatgaatgttcatcctacttgtcatattatagacatttgtcacaattcaagtgaggaagaaattctgtaattttctatgggctagttgctgcgttaactacaataatggggctgacatcctgaggttatagtgttcgattaccaagttctaatttattaagaggaatgcaagtcaacatgcatgcctagcccatttacttttaatgagtagtgtaacttctgttgcagaagtactaatgacagctttctatataaatttcacaggttgatttatcaaggacaacaattcgtcagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaaggtgaggaaactactcagcaaaattatacagtcatcattttgtgcacacttttagttatcagcaacattcgtatgtaggacgaatatatgatttgggaggacaagttatagcagcaaacagtgcccctgttataactcaccttgcagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggtgagttgttctagaataaaatttaaatttgaggtaactggataataagcatctaattatttataatattctctctatttcttgcatgcgtgttattcaataattcttacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactggtgagcatattttaaattcagtttggctggcattgaagctatagtatttcagttgccttattagtatgtatgatcttttgttcagatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaaggtatatatgttaataatatttctcctgttgcaaggtcttcttttttctcgtgataacattggtaatttcctgcattgcagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaaggtgagcaagaaataatggcgtaatatatctcaattattaattccaccttcatcaaatttcttagagaaattgtgcatatatttttccatacttgcagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagagg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ica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001187049.1 RefSeq:Os04g0473900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 4]]<br /> [[Category:Chromosome 4]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os04g0473900&diff=175251 2014-06-01T01:43:44Z

<p>Mayamei0424: /* Mutation */</p> <hr /> <div>''DWA1'' encodes an enzymatic megaprotein conserved in vascular plants，and ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''DWA1'' encodes an enzymatic megaprotein (2,391 aa in length) conserved in vascular plants, including an oxidoreductase-like domain, a prokaryotic nonribosomal peptide synthetase-like module, an AMP-binding domain, and an allene oxide synthase-like domain. The AMP-binding domain exhibits in vitro enzymatic activity in activating long-chain fatty acids to form acyl-CoA. Expression pattern analysis shows that ''DWA1'' is preferentially expressed in vascular tissues and epidermal layers and strongly induced by drought stress. The ''dwa1'' mutant was highly sensitive to drought stress compared to the WT and DWA1-functional complementation （DWA1-FC）plants restored drought resistance. Further dissections uncovers that the ''dwa1'' mutant was impaired in cuticular wax accumulation and significantly suppressed many wax-related genes expression under drought conditions and reduced levels of very-long-chain fatty acids. Conversely, plants overexpressing ''DWA1'' were significantly up-regulated many wax-related genes expression under drought conditions and elevated levels of very-long-chain fatty acids. The results suggest that ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice&lt;ref name=&quot;ref1&quot;/&gt;.<br /> <br /> ===Mutation===<br /> [[File:dwa1.jpg|right|thumb|200px|Fig.1 Screening of mutants(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa2.jpg|right|thumb|200px|Fig.2 phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa3.jpg|right|thumb|200px|Fig.3 functional complementation assay analysis(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa4.jpg|right|thumb|200px|Fig.4 Overexpression of ''DWA1'' analysis(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> '''(1)''' '''Screening of mutants(Identification of the drought-hypersensitive ''dwa1'' mutant in rice.)'''&lt;br&gt;<br /> ''dwa1'' mutant is observed by inserting T-DNA in the sixth exon of ''DWA1''(Fig.1A and B).RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1C and D).&lt;br&gt;<br /> '''(2)''' '''phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT'''&lt;br&gt;<br /> During the course of drought treatment, the mutant wilted and exhibited leaf rolling earlier than the WT. After severe drought treatment followed by rewatering, more than 90% of the WT plants survive, whereas the ''dwa1'' mutant plants are almost dead(Fig.2A and C). Water loss rate of mutant is faster than WT(Fig.2B).Furthermore, SEM analysis indicate that the deposition of vertical plate-like wax crystals on the ''dwa1'' leaf cuticle is severely reduced under normal and drought conditions in contrast to WT leaf(Fig.2D). The contents of individual FA components is obvious difference between the ''dwa1'' mutant and WT. Among them, VLCFAs are significantly reduced, whereas the levels of are slightly higher in the ''dwa1'' mutant(Fig.2E). The gene expression levels of wax-related genes are obviously different under drought treatment(Fig.2F)&lt;br&gt;<br /> '''(3) functional complementation assay analysis'''&lt;br&gt;<br /> DWA1-functional complementation(DWA1-FC)lines which is inserted the full-length genomic fragment of ''DWA1'' into the ''dwa1'' mutant restore drought resistance(Fig.3).&lt;br&gt;<br /> '''(4)''' '''Overexpression of ''DWA1'' analysis'''&lt;br&gt;<br /> There are three independent overexpression plants(U7,U9 and U10) and a negative control (U4),and there is no visual differences in the epicuticular wax load compared with the control (Fig. 4B).But the VLCFA constituents increase in the overexpression lines compared with WT. However, the contents of LCFAs are significantly reduced in the overexpression plants. The alkanes and primary alcohols showed no significant changes (Fig. 4C). Under normal growth conditions, the expression levels of wax-related genes enhance in the overexpression plants (Fig.4D).<br /> <br /> ===Expression===<br /> [[File:图片4.png|right|thumb|200px|Fig.2 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:图片5.png|right|thumb|200px|Fig.3 Drought-induced expression of ''DWA1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> '''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.2A).&lt;br&gt;<br /> '''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.2B 1-2), axillary bud (Fig.2B3), young panicle (Fig.2B4),spikelet (Fig.2B5), stem (Fig.2B6), young panicle (Fig.2B7), pistil (Fig.2B8), stamen (Fig.2B9), young embryo (Fig.2B10), SEM picture of rice leaf cuticle (Fig.2B11), cross-sections of stem (Fig.2B12) and leaf (Fig.2B13),and semithin sections of leaf (Fig.2B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.&lt;br&gt;<br /> '''(3)'''What's more,after various phytohormones treatment,''DWA1'' expresses higher,such as abscisic acid, jasmonic acid, indole-3-acetic acid, and<br /> gibberellic acid (Fig.2C).&lt;br&gt;<br /> '''(4)'''Under drought treatment,GUS staining shows that''DWA1'' is induced.For example,in Fig.3B,GUS staining in leaf before (1 and 3) and after (2 and 4) drought stress is shown.&lt;br&gt;<br /> <br /> ===Evolution===<br /> [[File:1(DWA1).png|right|thumb|200px|Fig.4 Unrooted phylogenetic tree and domain architecture of DWA1 protein orthologs in vascular plants and microbial relatives(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region&lt;ref name=&quot;ref1&quot;/&gt;(Fig.4).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi&lt;ref name=&quot;ref2&quot;/&gt;,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain&lt;ref name=&quot;ref1&quot;/&gt;.<br /> <br /> ==Labs working on this gene==<br /> *National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan,China<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Xiaoyi Zhu, Lizhong Xiong.（2013）Putative megaenzyme DWA1 plays essential roles in drought resistance by regulating stress-induced wax<br /> deposition in rice.PANS 110(44): 17790-17795.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Marahiel MA, Stachelhaus T, Mootz HD.(1997) Modular peptide synthetases involved in nonribosomal peptide synthesis.Chem Rev 97(7):2651–2674.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os04g0473900|<br /> Description = Adrenodoxin reductase family protein|<br /> Version = NM_001187049.1 GI:297723228 GeneID:9266980|<br /> Length = 9882 bp|<br /> Definition = Oryza sativa Japonica Group Os04g0473900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|<br /> AP = Chromosome 4:24097480..24107361|<br /> CDS = 24097480..24100068,24100784..24104053,24104151..24104329,24104410..24104769,24104855..24105296&lt;br&gt;,24105409..24105542,24105689..24105892,24107127..24107361|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008397:24097480..24107361<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:24097480..24107361<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactgatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttctggctcagaaaggcatctcaattccagattcttctgatcaggttggacttgttgtgattgcagaggttagagagggtaaggcagtctctaaagaggttgttaacaacatcaaagcacgtgtggtggaggagcatggtgtagctgttgcatctgtgaagcttatcaagcctagaaccatatgcaagacaacatctgggaagatacgaagatttgaatgcatgaggcagtttgttgacaatactcttagtttggctaagggcaaccatgtatctaagaaaaagggtctttttcggtctctgacaacaggaactggcatggagtcaaagaggtccctcttgaggcaaactgttgacctcaccattagccattggcccaaaagccaagttaaaaactccaatgaaataactgagtttctgacacaaatagtgtcagagcacactggcatttcaaaggataaaatttctctaactgacagtctaccttcgtatggttttgattctattgcagtggtacgagcagcccagaagctctcagattttcttggtgttcctgttggggccattgacatctttacagcaagctgcatttcggagcttgcaagcttcttggagaatttggtgcataagtcacaaacacagttggcaccttggcccaaaagcaaagtgaaaaactccaaggtgattatcgagtttctgacaaagattgtatcagatcaaacagggattccaaaggataaaatttctccgatggatagcctaccttcatatggttttgattcaattgcggtggtccaagcagcacagaaactctccgattttctcggtgtacctgttggagccattgacatatttacagctggttgcatttctgagctagctaccttcttggagaatttggcgcataagtcacagtcacagttggcacctggtgcatcttgctatattgaggatgagactcaagtggatcccatggatgccattagtccagaattcagtgttttgggtacgggcatcctgcaactgctggctcttacttatgtctgttttgtgctgctactcccagcatatcttgctagttcgacatacatgagcattttctctacagtcagtctggtcagatcaccattgctgagctatcttagttctttggttatggcacctattgtgtggatcttttatatctcgctcacttctttatcactgtctatcttggggaagtccttcctacagccaaattatgttctgatccctgatgtttcaatatggtcagttgactttgtgaaatggtgggcactaaacaaagcacaagcattagctgcaaagatgctagctgtgcatctaaagggcacaattttcttaaattattggttcaagatgcaaggagctcggattggttcatctgttgtcatcgacacagtagacataaccgatccatccctgttgacggttgcagatggtgcagttcttgcagaaggcgctcttgttcagggccatgaagtctgtaatgaagtgctgagttttaggcctatctggattggctgtgaggcttcgattggtccttatgcggtgcttcagaagggaactgttgtggaagatggagctgttgttccaccattgcagaagactggggctgggaaatcaacacgtcggactagcagaacttctgtaagcataaagaaagaagcagccaaagcaaacatgatacttgaacacctcgttagcatctacgcagttggcatcctgggtgctctgtccggtgcaatcgtctacacactatacactcacttgtccggcaaagcggcttcgcctctgcatttctcctttgcatgcattgctggtgcgttccactggttaccagctgccatcactgcctatgctgttattgtccaggagacacccacaagtgcactgtcatttgctctcttcactgccttcgccgacctgagctatggtgtcatcctcagtattctcaccagtataaccagcagagcccttgctgcaaagcctggaaccaagcagaatggcattgcgagtttgatccaccggcgcataaccatctctgctcatgtcaggtttgccaagatgctatctggaacagaagccttctgcgtgtacctgaggcttctcggagcaaagattggcaggcattgctctatccgagccatcaatccggtggcgaatcctgagctgatcagcgtcggtgatggcgtccatctaggtgacttctgcaatattgttccagggttctactccaaggggggattcaccagtgcagagattaaggtccaggagaacaccgtggttggcagtggcagcttgctcctccctggctgtgtcctccaggagaatgtcatcctcggcgcgctctccgtcgcgccggagaacgctgttctccggcgtggcggcgtctacgtcgggtcacagtctccggccatggtgaagaacaccttgcttgacgaggatgaacggatcgaagagatggatcaggcatacaagaagattgttggcaacctcgcggcgaacttggccatcaccaccatgaacgtcaagtcgaggtacttccaccgcatcggcgtgagcggccgcggcgtcctgaggatgtacgaggagataccgtcgtttccccggcacaagatcttcgcctccggcaagtccttcccggtgatcgtccgccacagcaacagcctgagcgccgacgacgacgcgaggctcgacgcccgcggcgccgcggtgcggatcctctcggacaacgacggcgaggcgcctctcctcgacctcacactcaagagcggcaaggcgttctacgcgcgcaccatcgccgacttcgccacctggctggtctgcggcctgccggcgagggaggagcaggtcaagcgctcgccgcacatccgcgacgccgtctggggctctctccggagcaccgactcgtacaccgtgctccactactactccaacatatgccggctgctgcggttcgacgacggcagggagatgtacgccaagttcaagctccgtcctgctgatccagacgtccccgaggactccggcaaggtggtgccgaggggcatcctgccgccggagaccggcgcgatccccagggacgaggatgacacgcgccctctgctcttcctcgccgacgacttccgccggagagtgggctcaccggacggggtgcgctacgtgttccagctgcagctcagggaggtgcccaccgacgccgccgcccgcgacgtcgccctcgactgcacccggccgtgggacgaggccgagttcccttacatcgacgtcggcgaggtcagcatcggccgcaacctcccgacggaggagacggagaagctcgagttcaacccgttcctccggtgccccgaggtggacgtcatcccggcgacgtcgtgcgcgcagagcgcgtccatcgaccacggccgctcgctggtctacgagatatgccagcgcctgcggaacggcgagccgctgcccgcgtcgtggcgagctttcctggagcagtcggacaccaagatcgacctgtcaggatgcccggtcgctgccgccgcgacacccacaaggtctaacgccggcgacgccacgaaggtgacgctggccaggacgtggtaccaggcgctctgggccacgctgtgccagccgctgctgcaaacgctggtgccgtactctgtcctgggcctcgtcatcttcctcccgctcaggggcctcctcgccgtcgccgcggccacccgcttcccgctctactggctcctcccggcgttctgggccgcgtcgggcgtcgccgccatggccacgtgcgcggcggcgaagtgggcgctggtcggcagcagggtcgacggcgacacggtgcacatctggtcgccggccgtgttcctggacacggtctggcaggccgtgcgcgccgccacggcggagtacttcgccgagctgacgcccgggtcggcgccgttcgcggcgtggatgcgggtgatgggcgcctccgtgtcccccggcgacggcgtgtacgtggactccatgggcgcgctgctgaacccggagatggtgcgcctggagcgcggcgccgccgtcgggcgggacgcgctgctgttcggccacgtgtacgagggcgaggccgggaaggtgaagttcggcgccgtgtccgtcggggaggacgggttcgtcggcagccgcgccgtggccatgcccagcgtcacggtggacgacggcggctgcctcgccgcgctcgggctggccatgaagggggagacggtgaagcacagcatgtaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MEERRATSPSRHSFCRDALGRRGGESGGGLVYIYRSPLRSPLQR HGLNREWRLERWRWRWRWEAATWRQRAAAHARIGEMDPKRSIDDQFSKLHPSLPVDTR IGIVGAGPSGLSAAYALAKLGYRNVTLFEKCHTVSGMCESIDIEEELGSDFEEMDTHK LSLIDSQTGNIRDLEVAEDYVSMVSLTLKLQDEANKSGRAGLHALSGLASDPTHEFLK QNGINSMPKSVAYGYTASGYGFVQDMPYAFIQEFTRTSMAGKIRRFKHGYMSMWERLS KSLPFEVFCDTQVLNVKRNSCGANVTIKNNNGEKQVLEFDKIILSGAVAFKNSKTYRS SSLTDGESEVVELNNLERELFSKVQTIDYYTTVVKINGFEHIPKGFYYFGEFMEDPTT IGHPVAMQRFFADTNIFLFWSYGNSADIKGSFVAKCVTDVVSSMGGNVQKVILQRRFK YFPHVSSKDMKDGFYERLESQLQGFQNTYYVGGLLAFELTERNASYSIMAVCKHFAID GEGELTPYVKRLFPLSHNRNPSPPRDLGELEGVEFPDLPSLDGYLQYWGTHKVTEKNV IYTWINEEGKLMNRRTYQELHGNASYIAQKLLTSTKPVIKPGDRVLLIHLPGLEFIDA FFGCIRAGVIPVPVLPPDPMQSGGQALLKVENISKMCNAVAILSTSSYHAAVRAGYIK NIVTLAKRVQKCSAQWPDIPWIHTDSWIKNYRRSSDSFNSDTVLFTKPQPSDLCFLQF TSGSTGDAKGVMITHEGLIHNVKTMKKRYRSTSKTVLVSWLPQYHDMGLIGGLFTALV SGGTSVLFSPMIFIRNPLLWLQTINDYHGTHSAGPNFAFELVIRRLEAEKNKVYDLSS MVFLMIAAEPVRQKTVRRFIELTQPFGLSEGVLAPGYGLAENCVYVTCAFGECKPVFI DWQGRVCCGYVEQDDTDTLIRIVDPDSLTEHQEDGVEGEIWISSPSSGVGYWGNSEMS QRTFFNQLKNHPNKKFTRTGDLGRTIDGNLFITGRIKDLIIVAGRNIYSADVEKTVES SSEVLRPGCCAVVGIPEEVLAQKGISIPDSSDQVGLVVIAEVREGKAVSKEVVNNIKA RVVEEHGVAVASVKLIKPRTICKTTSGKIRRFECMRQFVDNTLSLAKGNHVSKKKGLF RSLTTGTGMESKRSLLRQTVDLTISHWPKSQVKNSNEITEFLTQIVSEHTGISKDKIS LTDSLPSYGFDSIAVVRAAQKLSDFLGVPVGAIDIFTASCISELASFLENLVHKSQTQ LAPWPKSKVKNSKVIIEFLTKIVSDQTGIPKDKISPMDSLPSYGFDSIAVVQAAQKLS DFLGVPVGAIDIFTAGCISELATFLENLAHKSQSQLAPGASCYIEDETQVDPMDAISP EFSVLGTGILQLLALTYVCFVLLLPAYLASSTYMSIFSTVSLVRSPLLSYLSSLVMAP IVWIFYISLTSLSLSILGKSFLQPNYVLIPDVSIWSVDFVKWWALNKAQALAAKMLAV HLKGTIFLNYWFKMQGARIGSSVVIDTVDITDPSLLTVADGAVLAEGALVQGHEVCNE VLSFRPIWIGCEASIGPYAVLQKGTVVEDGAVVPPLQKTGAGKSTRRTSRTSVSIKKE AAKANMILEHLVSIYAVGILGALSGAIVYTLYTHLSGKAASPLHFSFACIAGAFHWLP AAITAYAVIVQETPTSALSFALFTAFADLSYGVILSILTSITSRALAAKPGTKQNGIA SLIHRRITISAHVRFAKMLSGTEAFCVYLRLLGAKIGRHCSIRAINPVANPELISVGD GVHLGDFCNIVPGFYSKGGFTSAEIKVQENTVVGSGSLLLPGCVLQENVILGALSVAP ENAVLRRGGVYVGSQSPAMVKNTLLDEDERIEEMDQAYKKIVGNLAANLAITTMNVKS RYFHRIGVSGRGVLRMYEEIPSFPRHKIFASGKSFPVIVRHSNSLSADDDARLDARGA AVRILSDNDGEAPLLDLTLKSGKAFYARTIADFATWLVCGLPAREEQVKRSPHIRDAV WGSLRSTDSYTVLHYYSNICRLLRFDDGREMYAKFKLRPADPDVPEDSGKVVPRGILP PETGAIPRDEDDTRPLLFLADDFRRRVGSPDGVRYVFQLQLREVPTDAAARDVALDCT RPWDEAEFPYIDVGEVSIGRNLPTEETEKLEFNPFLRCPEVDVIPATSCAQSASIDHG RSLVYEICQRLRNGEPLPASWRAFLEQSDTKIDLSGCPVAAAATPTRSNAGDATKVTL ARTWYQALWATLCQPLLQTLVPYSVLGLVIFLPLRGLLAVAAATRFPLYWLLPAFWAA SGVAAMATCAAAKWALVGSRVDGDTVHIWSPAVFLDTVWQAVRAATAEYFAELTPGSA PFAAWMRVMGASVSPGDGVYVDSMGALLNPEMVRLERGAAVGRDALLFGHVYEGEAGK VKFGAVSVGEDGFVGSRAVAMPSVTVDDGGCLAALGLAMKGETVKHSM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;7294..9882#3309..6578#3033..3211#2593..2952#2066..2507#1820..1953#1470..1673#1..235#atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggaggtatggattccccttccccactcgctgcgtgatgaaccgatgaaccgtagtacttgatgatatgcctgatttgcttcggcattgtgccgcttctctgttagctgggagtttgtggcgtgtcgtttaactgaagtagagatgtgatgatgatctgaggtgagcgccattttttttttttggctaactctgtttgttctattcagtgaatatgagctctcttttttgtcccctttttatcattctgcttctatgggtcgcggagcgttcttggttggtgcagtgcaactgcaatgtcagtatgcatgccatagtaatgtgatggtgggatgtaatgctcctctgcttgctcgagctctttcttggttgtctatctatcttggatgatttgatcaatgtactacttattatctgctgatagtacccggggtccatttgcgctagtattgaacttcttcttctcttataatttgctgaaatcgcgagattatcgcttaggtttatcaatcagaacattatcatgtttatgcttcggttcgttcggtactggcacatgtcaaataagcggaaattctgctactttttcggcttaccatgaatggcacccaacatagcgtggttgttttgtcatgttgaactagtacaaatttttgcaccttaatatttgctttccgtgcatatccaattaacctgcccatcaaacttcttttcagatagctattattggggaaaaatattttcccagcttcttttatagtgactagactgtttatacatgcctgcgtaattgcttccttttgatgagctagtacattatagaccttgattttgttttgatggtacgggttggttattggtgtccatataatgaaattgcaacttgcgaccactccatgatagttacctggtcattccttgttggttaataagctattatttcaaatatgaatgttcatcctacttgtcatattatagacatttgtcacaattcaagtgaggaagaaattctgtaattttctatgggctagttgctgcgttaactacaataatggggctgacatcctgaggttatagtgttcgattaccaagttctaatttattaagaggaatgcaagtcaacatgcatgcctagcccatttacttttaatgagtagtgtaacttctgttgcagaagtactaatgacagctttctatataaatttcacaggttgatttatcaaggacaacaattcgtcagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaaggtgaggaaactactcagcaaaattatacagtcatcattttgtgcacacttttagttatcagcaacattcgtatgtaggacgaatatatgatttgggaggacaagttatagcagcaaacagtgcccctgttataactcaccttgcagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggtgagttgttctagaataaaatttaaatttgaggtaactggataataagcatctaattatttataatattctctctatttcttgcatgcgtgttattcaataattcttacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactggtgagcatattttaaattcagtttggctggcattgaagctatagtatttcagttgccttattagtatgtatgatcttttgttcagatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaaggtatatatgttaataatatttctcctgttgcaaggtcttcttttttctcgtgataacattggtaatttcctgcattgcagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaaggtgagcaagaaataatggcgtaatatatctcaattattaattccaccttcatcaaatttcttagagaaattgtgcatatatttttccatacttgcagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagagg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ica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001187049.1 RefSeq:Os04g0473900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 4]]<br /> [[Category:Chromosome 4]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os04g0473900&diff=175250 2014-06-01T01:37:49Z

<p>Mayamei0424: /* Mutation */</p> <hr /> <div>''DWA1'' encodes an enzymatic megaprotein conserved in vascular plants，and ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''DWA1'' encodes an enzymatic megaprotein (2,391 aa in length) conserved in vascular plants, including an oxidoreductase-like domain, a prokaryotic nonribosomal peptide synthetase-like module, an AMP-binding domain, and an allene oxide synthase-like domain. The AMP-binding domain exhibits in vitro enzymatic activity in activating long-chain fatty acids to form acyl-CoA. Expression pattern analysis shows that ''DWA1'' is preferentially expressed in vascular tissues and epidermal layers and strongly induced by drought stress. The ''dwa1'' mutant was highly sensitive to drought stress compared to the WT and DWA1-functional complementation （DWA1-FC）plants restored drought resistance. Further dissections uncovers that the ''dwa1'' mutant was impaired in cuticular wax accumulation and significantly suppressed many wax-related genes expression under drought conditions and reduced levels of very-long-chain fatty acids. Conversely, plants overexpressing ''DWA1'' were significantly up-regulated many wax-related genes expression under drought conditions and elevated levels of very-long-chain fatty acids. The results suggest that ''DWA1'' controls drought resistance by regulating drought-induced cuticular wax deposition in rice&lt;ref name=&quot;ref1&quot;/&gt;.<br /> <br /> ===Mutation===<br /> [[File:dwa1.jpg|right|thumb|200px|Fig.1 Screening of mutants(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa2.jpg|right|thumb|200px|Fig.2 phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa3.jpg|right|thumb|200px|Fig.3 functional complementation assay analysis(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:dwa4.jpg|right|thumb|200px|Fig.4 Overexpression of ''DWA1'' analysis(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> ''(1)'' '''Screening of mutants(Identification of the drought-hypersensitive ''dwa1'' mutant in rice.)'''<br /> ''dwa1'' mutant is observed by inserting T-DNA in the sixth exon of ''DWA1''(Fig.1A and B).RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1C and D).<br /> ''(2)'' ''phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT''<br /> During the course of drought treatment, the mutant wilted and exhibited leaf rolling earlier than the WT. After severe drought treatment followed by rewatering, more than 90% of the WT plants survive, whereas the ''dwa1'' mutant plants are almost dead(Fig.2A and C). Water loss rate of mutant is faster than WT(Fig.2B).Furthermore, SEM analysis indicate that the deposition of vertical plate-like wax crystals on the ''dwa1'' leaf cuticle is severely reduced under normal and drought conditions in contrast to WT leaf(Fig.2D). The contents of individual FA components is obvious difference between the ''dwa1'' mutant and WT. Among them, VLCFAs are significantly reduced, whereas the levels of are slightly higher in the ''dwa1'' mutant(Fig.2E). The gene expression levels of wax-related genes are obviously different under drought treatment(Fig.2F).<br /> ''(3)'' ''functional complementation assay analysis''<br /> DWA1-functional complementation(DWA1-FC)lines which is inserted the full-length genomic fragment of ''DWA1'' into the ''dwa1'' mutant restore drought resistance(Fig.3). <br /> ''(4)'' '''Overexpression of ''DWA1'' analysis'''<br /> There are three independent overexpression plants(U7,U9 and U10) and a negative control (U4),and there is no visual differences in the epicuticular wax load compared with the control (Fig. 4B).But the VLCFA constituents increase in the overexpression lines compared with WT. However, the contents of LCFAs are significantly reduced in the overexpression plants. The alkanes and primary alcohols showed no significant changes (Fig. 4C). Under normal growth conditions, the expression levels of wax-related genes enhance in the overexpression plants (Fig.4D).<br /> <br /> ===Expression===<br /> [[File:图片4.png|right|thumb|200px|Fig.2 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> [[File:图片5.png|right|thumb|200px|Fig.3 Drought-induced expression of ''DWA1''.(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> '''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.2A).&lt;br&gt;<br /> '''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.2B 1-2), axillary bud (Fig.2B3), young panicle (Fig.2B4),spikelet (Fig.2B5), stem (Fig.2B6), young panicle (Fig.2B7), pistil (Fig.2B8), stamen (Fig.2B9), young embryo (Fig.2B10), SEM picture of rice leaf cuticle (Fig.2B11), cross-sections of stem (Fig.2B12) and leaf (Fig.2B13),and semithin sections of leaf (Fig.2B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.&lt;br&gt;<br /> '''(3)'''What's more,after various phytohormones treatment,''DWA1'' expresses higher,such as abscisic acid, jasmonic acid, indole-3-acetic acid, and<br /> gibberellic acid (Fig.2C).&lt;br&gt;<br /> '''(4)'''Under drought treatment,GUS staining shows that''DWA1'' is induced.For example,in Fig.3B,GUS staining in leaf before (1 and 3) and after (2 and 4) drought stress is shown.&lt;br&gt;<br /> <br /> ===Evolution===<br /> [[File:1(DWA1).png|right|thumb|200px|Fig.4 Unrooted phylogenetic tree and domain architecture of DWA1 protein orthologs in vascular plants and microbial relatives(from reference&lt;ref name=&quot;ref1&quot; /&gt;)]]<br /> At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region&lt;ref name=&quot;ref1&quot;/&gt;(Fig.4).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi&lt;ref name=&quot;ref2&quot;/&gt;,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain&lt;ref name=&quot;ref1&quot;/&gt;.<br /> <br /> ==Labs working on this gene==<br /> *National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan,China<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Xiaoyi Zhu, Lizhong Xiong.（2013）Putative megaenzyme DWA1 plays essential roles in drought resistance by regulating stress-induced wax<br /> deposition in rice.PANS 110(44): 17790-17795.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Marahiel MA, Stachelhaus T, Mootz HD.(1997) Modular peptide synthetases involved in nonribosomal peptide synthesis.Chem Rev 97(7):2651–2674.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os04g0473900|<br /> Description = Adrenodoxin reductase family protein|<br /> Version = NM_001187049.1 GI:297723228 GeneID:9266980|<br /> Length = 9882 bp|<br /> Definition = Oryza sativa Japonica Group Os04g0473900, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|<br /> AP = Chromosome 4:24097480..24107361|<br /> CDS = 24097480..24100068,24100784..24104053,24104151..24104329,24104410..24104769,24104855..24105296&lt;br&gt;,24105409..24105542,24105689..24105892,24107127..24107361|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008397:24097480..24107361<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:24097480..24107361<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactgatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttctggctcagaaaggcatctcaattccagattcttctgatcaggttggacttgttgtgattgcagaggttagagagggtaaggcagtctctaaagaggttgttaacaacatcaaagcacgtgtggtggaggagcatggtgtagctgttgcatctgtgaagcttatcaagcctagaaccatatgcaagacaacatctgggaagatacgaagatttgaatgcatgaggcagtttgttgacaatactcttagtttggctaagggcaaccatgtatctaagaaaaagggtctttttcggtctctgacaacaggaactggcatggagtcaaagaggtccctcttgaggcaaactgttgacctcaccattagccattggcccaaaagccaagttaaaaactccaatgaaataactgagtttctgacacaaatagtgtcagagcacactggcatttcaaaggataaaatttctctaactgacagtctaccttcgtatggttttgattctattgcagtggtacgagcagcccagaagctctcagattttcttggtgttcctgttggggccattgacatctttacagcaagctgcatttcggagcttgcaagcttcttggagaatttggtgcataagtcacaaacacagttggcaccttggcccaaaagcaaagtgaaaaactccaaggtgattatcgagtttctgacaaagattgtatcagatcaaacagggattccaaaggataaaatttctccgatggatagcctaccttcatatggttttgattcaattgcggtggtccaagcagcacagaaactctccgattttctcggtgtacctgttggagccattgacatatttacagctggttgcatttctgagctagctaccttcttggagaatttggcgcataagtcacagtcacagttggcacctggtgcatcttgctatattgaggatgagactcaagtggatcccatggatgccattagtccagaattcagtgttttgggtacgggcatcctgcaactgctggctcttacttatgtctgttttgtgctgctactcccagcatatcttgctagttcgacatacatgagcattttctctacagtcagtctggtcagatcaccattgctgagctatcttagttctttggttatggcacctattgtgtggatcttttatatctcgctcacttctttatcactgtctatcttggggaagtccttcctacagccaaattatgttctgatccctgatgtttcaatatggtcagttgactttgtgaaatggtgggcactaaacaaagcacaagcattagctgcaaagatgctagctgtgcatctaaagggcacaattttcttaaattattggttcaagatgcaaggagctcggattggttcatctgttgtcatcgacacagtagacataaccgatccatccctgttgacggttgcagatggtgcagttcttgcagaaggcgctcttgttcagggccatgaagtctgtaatgaagtgctgagttttaggcctatctggattggctgtgaggcttcgattggtccttatgcggtgcttcagaagggaactgttgtggaagatggagctgttgttccaccattgcagaagactggggctgggaaatcaacacgtcggactagcagaacttctgtaagcataaagaaagaagcagccaaagcaaacatgatacttgaacacctcgttagcatctacgcagttggcatcctgggtgctctgtccggtgcaatcgtctacacactatacactcacttgtccggcaaagcggcttcgcctctgcatttctcctttgcatgcattgctggtgcgttccactggttaccagctgccatcactgcctatgctgttattgtccaggagacacccacaagtgcactgtcatttgctctcttcactgccttcgccgacctgagctatggtgtcatcctcagtattctcaccagtataaccagcagagcccttgctgcaaagcctggaaccaagcagaatggcattgcgagtttgatccaccggcgcataaccatctctgctcatgtcaggtttgccaagatgctatctggaacagaagccttctgcgtgtacctgaggcttctcggagcaaagattggcaggcattgctctatccgagccatcaatccggtggcgaatcctgagctgatcagcgtcggtgatggcgtccatctaggtgacttctgcaatattgttccagggttctactccaaggggggattcaccagtgcagagattaaggtccaggagaacaccgtggttggcagtggcagcttgctcctccctggctgtgtcctccaggagaatgtcatcctcggcgcgctctccgtcgcgccggagaacgctgttctccggcgtggcggcgtctacgtcgggtcacagtctccggccatggtgaagaacaccttgcttgacgaggatgaacggatcgaagagatggatcaggcatacaagaagattgttggcaacctcgcggcgaacttggccatcaccaccatgaacgtcaagtcgaggtacttccaccgcatcggcgtgagcggccgcggcgtcctgaggatgtacgaggagataccgtcgtttccccggcacaagatcttcgcctccggcaagtccttcccggtgatcgtccgccacagcaacagcctgagcgccgacgacgacgcgaggctcgacgcccgcggcgccgcggtgcggatcctctcggacaacgacggcgaggcgcctctcctcgacctcacactcaagagcggcaaggcgttctacgcgcgcaccatcgccgacttcgccacctggctggtctgcggcctgccggcgagggaggagcaggtcaagcgctcgccgcacatccgcgacgccgtctggggctctctccggagcaccgactcgtacaccgtgctccactactactccaacatatgccggctgctgcggttcgacgacggcagggagatgtacgccaagttcaagctccgtcctgctgatccagacgtccccgaggactccggcaaggtggtgccgaggggcatcctgccgccggagaccggcgcgatccccagggacgaggatgacacgcgccctctgctcttcctcgccgacgacttccgccggagagtgggctcaccggacggggtgcgctacgtgttccagctgcagctcagggaggtgcccaccgacgccgccgcccgcgacgtcgccctcgactgcacccggccgtgggacgaggccgagttcccttacatcgacgtcggcgaggtcagcatcggccgcaacctcccgacggaggagacggagaagctcgagttcaacccgttcctccggtgccccgaggtggacgtcatcccggcgacgtcgtgcgcgcagagcgcgtccatcgaccacggccgctcgctggtctacgagatatgccagcgcctgcggaacggcgagccgctgcccgcgtcgtggcgagctttcctggagcagtcggacaccaagatcgacctgtcaggatgcccggtcgctgccgccgcgacacccacaaggtctaacgccggcgacgccacgaaggtgacgctggccaggacgtggtaccaggcgctctgggccacgctgtgccagccgctgctgcaaacgctggtgccgtactctgtcctgggcctcgtcatcttcctcccgctcaggggcctcctcgccgtcgccgcggccacccgcttcccgctctactggctcctcccggcgttctgggccgcgtcgggcgtcgccgccatggccacgtgcgcggcggcgaagtgggcgctggtcggcagcagggtcgacggcgacacggtgcacatctggtcgccggccgtgttcctggacacggtctggcaggccgtgcgcgccgccacggcggagtacttcgccgagctgacgcccgggtcggcgccgttcgcggcgtggatgcgggtgatgggcgcctccgtgtcccccggcgacggcgtgtacgtggactccatgggcgcgctgctgaacccggagatggtgcgcctggagcgcggcgccgccgtcgggcgggacgcgctgctgttcggccacgtgtacgagggcgaggccgggaaggtgaagttcggcgccgtgtccgtcggggaggacgggttcgtcggcagccgcgccgtggccatgcccagcgtcacggtggacgacggcggctgcctcgccgcgctcgggctggccatgaagggggagacggtgaagcacagcatgtaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MEERRATSPSRHSFCRDALGRRGGESGGGLVYIYRSPLRSPLQR HGLNREWRLERWRWRWRWEAATWRQRAAAHARIGEMDPKRSIDDQFSKLHPSLPVDTR IGIVGAGPSGLSAAYALAKLGYRNVTLFEKCHTVSGMCESIDIEEELGSDFEEMDTHK LSLIDSQTGNIRDLEVAEDYVSMVSLTLKLQDEANKSGRAGLHALSGLASDPTHEFLK QNGINSMPKSVAYGYTASGYGFVQDMPYAFIQEFTRTSMAGKIRRFKHGYMSMWERLS KSLPFEVFCDTQVLNVKRNSCGANVTIKNNNGEKQVLEFDKIILSGAVAFKNSKTYRS SSLTDGESEVVELNNLERELFSKVQTIDYYTTVVKINGFEHIPKGFYYFGEFMEDPTT IGHPVAMQRFFADTNIFLFWSYGNSADIKGSFVAKCVTDVVSSMGGNVQKVILQRRFK YFPHVSSKDMKDGFYERLESQLQGFQNTYYVGGLLAFELTERNASYSIMAVCKHFAID GEGELTPYVKRLFPLSHNRNPSPPRDLGELEGVEFPDLPSLDGYLQYWGTHKVTEKNV IYTWINEEGKLMNRRTYQELHGNASYIAQKLLTSTKPVIKPGDRVLLIHLPGLEFIDA FFGCIRAGVIPVPVLPPDPMQSGGQALLKVENISKMCNAVAILSTSSYHAAVRAGYIK NIVTLAKRVQKCSAQWPDIPWIHTDSWIKNYRRSSDSFNSDTVLFTKPQPSDLCFLQF TSGSTGDAKGVMITHEGLIHNVKTMKKRYRSTSKTVLVSWLPQYHDMGLIGGLFTALV SGGTSVLFSPMIFIRNPLLWLQTINDYHGTHSAGPNFAFELVIRRLEAEKNKVYDLSS MVFLMIAAEPVRQKTVRRFIELTQPFGLSEGVLAPGYGLAENCVYVTCAFGECKPVFI DWQGRVCCGYVEQDDTDTLIRIVDPDSLTEHQEDGVEGEIWISSPSSGVGYWGNSEMS QRTFFNQLKNHPNKKFTRTGDLGRTIDGNLFITGRIKDLIIVAGRNIYSADVEKTVES SSEVLRPGCCAVVGIPEEVLAQKGISIPDSSDQVGLVVIAEVREGKAVSKEVVNNIKA RVVEEHGVAVASVKLIKPRTICKTTSGKIRRFECMRQFVDNTLSLAKGNHVSKKKGLF RSLTTGTGMESKRSLLRQTVDLTISHWPKSQVKNSNEITEFLTQIVSEHTGISKDKIS LTDSLPSYGFDSIAVVRAAQKLSDFLGVPVGAIDIFTASCISELASFLENLVHKSQTQ LAPWPKSKVKNSKVIIEFLTKIVSDQTGIPKDKISPMDSLPSYGFDSIAVVQAAQKLS DFLGVPVGAIDIFTAGCISELATFLENLAHKSQSQLAPGASCYIEDETQVDPMDAISP EFSVLGTGILQLLALTYVCFVLLLPAYLASSTYMSIFSTVSLVRSPLLSYLSSLVMAP IVWIFYISLTSLSLSILGKSFLQPNYVLIPDVSIWSVDFVKWWALNKAQALAAKMLAV HLKGTIFLNYWFKMQGARIGSSVVIDTVDITDPSLLTVADGAVLAEGALVQGHEVCNE VLSFRPIWIGCEASIGPYAVLQKGTVVEDGAVVPPLQKTGAGKSTRRTSRTSVSIKKE AAKANMILEHLVSIYAVGILGALSGAIVYTLYTHLSGKAASPLHFSFACIAGAFHWLP AAITAYAVIVQETPTSALSFALFTAFADLSYGVILSILTSITSRALAAKPGTKQNGIA SLIHRRITISAHVRFAKMLSGTEAFCVYLRLLGAKIGRHCSIRAINPVANPELISVGD GVHLGDFCNIVPGFYSKGGFTSAEIKVQENTVVGSGSLLLPGCVLQENVILGALSVAP ENAVLRRGGVYVGSQSPAMVKNTLLDEDERIEEMDQAYKKIVGNLAANLAITTMNVKS RYFHRIGVSGRGVLRMYEEIPSFPRHKIFASGKSFPVIVRHSNSLSADDDARLDARGA AVRILSDNDGEAPLLDLTLKSGKAFYARTIADFATWLVCGLPAREEQVKRSPHIRDAV WGSLRSTDSYTVLHYYSNICRLLRFDDGREMYAKFKLRPADPDVPEDSGKVVPRGILP PETGAIPRDEDDTRPLLFLADDFRRRVGSPDGVRYVFQLQLREVPTDAAARDVALDCT RPWDEAEFPYIDVGEVSIGRNLPTEETEKLEFNPFLRCPEVDVIPATSCAQSASIDHG RSLVYEICQRLRNGEPLPASWRAFLEQSDTKIDLSGCPVAAAATPTRSNAGDATKVTL ARTWYQALWATLCQPLLQTLVPYSVLGLVIFLPLRGLLAVAAATRFPLYWLLPAFWAA SGVAAMATCAAAKWALVGSRVDGDTVHIWSPAVFLDTVWQAVRAATAEYFAELTPGSA PFAAWMRVMGASVSPGDGVYVDSMGALLNPEMVRLERGAAVGRDALLFGHVYEGEAGK VKFGAVSVGEDGFVGSRAVAMPSVTVDDGGCLAALGLAMKGETVKHSM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;7294..9882#3309..6578#3033..3211#2593..2952#2066..2507#1820..1953#1470..1673#1..235#atggaggagcggcgcgcgacgtcgccgtcgcggcacagcttttgtcgcgacgcccttggccgccgcgggggggaatctgggggcggcctcgtatatatataccgtagcccgctgaggtcaccgctccaacgccacggcctcaaccgggagtggagactggagaggtggaggtggaggtggaggtgggaagctgccacttggcgccagcgcgccgctgcccacgctcggatcggaggtatggattccccttccccactcgctgcgtgatgaaccgatgaaccgtagtacttgatgatatgcctgatttgcttcggcattgtgccgcttctctgttagctgggagtttgtggcgtgtcgtttaactgaagtagagatgtgatgatgatctgaggtgagcgccattttttttttttggctaactctgtttgttctattcagtgaatatgagctctcttttttgtcccctttttatcattctgcttctatgggtcgcggagcgttcttggttggtgcagtgcaactgcaatgtcagtatgcatgccatagtaatgtgatggtgggatgtaatgctcctctgcttgctcgagctctttcttggttgtctatctatcttggatgatttgatcaatgtactacttattatctgctgatagtacccggggtccatttgcgctagtattgaacttcttcttctcttataatttgctgaaatcgcgagattatcgcttaggtttatcaatcagaacattatcatgtttatgcttcggttcgttcggtactggcacatgtcaaataagcggaaattctgctactttttcggcttaccatgaatggcacccaacatagcgtggttgttttgtcatgttgaactagtacaaatttttgcaccttaatatttgctttccgtgcatatccaattaacctgcccatcaaacttcttttcagatagctattattggggaaaaatattttcccagcttcttttatagtgactagactgtttatacatgcctgcgtaattgcttccttttgatgagctagtacattatagaccttgattttgttttgatggtacgggttggttattggtgtccatataatgaaattgcaacttgcgaccactccatgatagttacctggtcattccttgttggttaataagctattatttcaaatatgaatgttcatcctacttgtcatattatagacatttgtcacaattcaagtgaggaagaaattctgtaattttctatgggctagttgctgcgttaactacaataatggggctgacatcctgaggttatagtgttcgattaccaagttctaatttattaagaggaatgcaagtcaacatgcatgcctagcccatttacttttaatgagtagtgtaacttctgttgcagaagtactaatgacagctttctatataaatttcacaggttgatttatcaaggacaacaattcgtcagaaatggacccaaagagatcaatagatgatcaattttctaagttgcatccaagtttaccagttgatactagaattggcatagtgggagcaggaccaagtggcttatcagcagcctatgcactagctaagctaggttaccgcaatgtaactttgtttgagaaatgccatactgtttcagggatgtgtgagtccattgacattgaaggtgaggaaactactcagcaaaattatacagtcatcattttgtgcacacttttagttatcagcaacattcgtatgtaggacgaatatatgatttgggaggacaagttatagcagcaaacagtgcccctgttataactcaccttgcagaggaactagggtctgatttcgaagagatggatacacacaaactctccctgattgacagccaaacaggaaatattcgagatcttgaagtagctgaggactatgtatccatggtatctctgacgctaaaattacaggtgagttgttctagaataaaatttaaatttgaggtaactggataataagcatctaattatttataatattctctctatttcttgcatgcgtgttattcaataattcttacaggatgaagcaaataagtcaggccgagctggacttcatgctctcagtggattggcatccgacccgacccatgaatttttgaagcaaaatggaataaattcaatgccaaaatctgtagcatatggttacaccgcttcgggctatggttttgttcaagacatgccctatgctttcattcaggagtttactagaacctctatggctggtaaaatccggcgtttcaagcatggttacatgagcatgtgggagagattaagtaaatcattaccatttgaagttttttgtgacacgcaagttctgaatgtcaagcgcaacagttgtggtgcaaatgtaaccatcaagaacaacaatggtgaaaaacaagttttggagttcgacaaaattatactatcaggtgctgttgctttcaagaacagcaaaacatatcgatcctctagtcttactggtgagcatattttaaattcagtttggctggcattgaagctatagtatttcagttgccttattagtatgtatgatcttttgttcagatggagaaagtgaagtggtggaactaaacaatcttgaaagagaactattcagtaaagtacagactatcgactactatactactgttgttaagatcaatggattcgagcacataccaaagggattttactactttggagaattcatggaggatccaacaacaataggacaccctgtggcaatgcaaagattctttgctgacacaaacattttcttgttttggtcttatggaaattcagcagacatcaagggttcatttgtggctaaatgtgtgactgatgtcgtgagttctatgggaggcaatgttcagaaagtaattttgcaacggcggttcaagtatttccctcatgtcagcagtaaaggtatatatgttaataatatttctcctgttgcaaggtcttcttttttctcgtgataacattggtaatttcctgcattgcagacatgaaagatggattctatgagaggttggagtcccagttacaaggttttcagaacacttactatgttggaggtttgctagcatttgagcttaccgaaagaaacgcttcgtactccatcatggctgtttgtaagcactttgccattgatggtgagggagaactgactccttatgtcaaggtgagcaagaaataatggcgtaatatatctcaattattaattccaccttcatcaaatttcttagagaaattgtgcatatatttttccatacttgcagagattattccctttgtcccataatagaaatccatcacctcctagagatcttggtgagcttgaaggtgttgaatttcctgaccttccctcccttgatggatatttgcaatactggggaactcataaagtcactgagaagaatgttatctatacttggatcaatgaagaaggaaagctcatgaatcgaagaacctaccaggagcttcatgggaatgcatcttacattgcacaaaaacttttaacaagcacaaaacctgttatcaagcctggtgatagagttctgcttattcacctccctggtttggagtttattgatgcattctttgggtgtattagagcaggagttataccagtccctgtattgccccctgatccaatgcaaagtggtggacaagctcttcttaaagtcgagaacatctctaagatgtgcaatgctgtggctattctatccacctcttcgtaccatgctgctgtacgagcaggttatatcaagaacattgtcactttagcaaagagagttcagaaatgctcagcacagtggcctgacatcccatggattcataccgactcatggataaagaactaccggcgaagctcagacagcttcaactcagacactgttttatttaccaaaccccagccaagtgacttgtgctttctacagttcacttcaggttccactggtgatgccaagggggtgatgataactcatgaaggacttatccacaacgtgaagactatgaaaaagcgttatagaagtacttcaaaaactgtccttgtcagttggcttccacagtatcatgacatgggtcttataggaggtcttttcactgctcttgtaagtggaggaacttctgttctattttcaccaatgatatttatcagaaacccccttctgtggttgcaaacgatcaatgactaccatggaacccatagtgcaggtccaaattttgcatttgaactcgtgataagaaggcttgaggctgagaagaacaaagtatatgacctctcatcaatggtcttcctcatgattgcggcagagccagtaaggcaaaaaactgtcagaaggttcatagagttaactcaaccttttggcctttctgaaggtgtcctcgcacctggatatggtctggctgagaactgtgtctatgttacctgtgccttcggtgaatgcaagccagttttcatagactggcaaggaagagtatgttgtgggtatgttgagcaagatgatacagacactctcatcagaattgttgatcctgactctctaactgagcaccaagaagatggtgttgaaggtgagatatggattagcagtcctagttctggagtaggttattggggcaattcggaaatgagccagagaacttttttcaatcagcttaagaaccaccccaacaagaagttcacaagaaccggtgatttgggacggaccatcgatggtaacctttttattacaggaaggatcaaagatctcatcattgtcgctggtagaaatatttattctgcggatgtagagaagactgtggaaagttcttctgaggtcctccgaccaggctgttgtgctgtggtcggtatcccagaggaagttctggctcagaaaggcatctcaattccagattcttctgatcaggttggacttgttgtgattgcagaggttagagagggtaaggcagtctctaaagaggttgttaacaacatcaaagcacgtgtggtggaggagcatggtgtagctgttgcatctgtgaagcttatcaagcctagaaccatatgcaagacaacatctgggaagatacgaagatttgaatgcatgaggcagtttgttgacaatactcttagtttggctaagggcaaccatgtatctaagaaaaagggtctttttcggtctctgacaacaggaactggcatggagtcaaagaggtccctcttgaggcaaactgttgacctcaccattagccattggcccaaaagccaagttaaaaactccaatgaaataactgagtttctgacacaaatagtgtcagagcacactggcatttcaaaggataaaatttctctaactgacagtctaccttcgtatggttttgattctattgcagtggtacgagcagcccagaagctctcagattttcttggtgttcctgttggggccattgacatctttacagcaagctgcatttcggagcttgcaagcttcttggagaatttggtgcataagtcacaaacacagttggcaccttggcccaaaagcaaagtgaaaaactccaaggtgattatcgagtttctgacaaagattgtatcagatcaaacagggattccaaaggataaaatttctccgatggatagcctaccttcatatggttttgattcaattgcggtggtccaagcagcacagaaactctccgattttctcggtgtacctgttggagccattgacatatttacagctggttgcatttctgagctagctaccttcttggagaatttggcgcataagtcacagtcacagttggcacctggtgcatcttgctatattgaggatgagactcaagtggatcccatggatgccattagtccagaattcagtgttttgggtacgggcatcctgcaactgctggctcttacttatgtctgttttgtgctgctactcccagcatatcttgctagttcgacatacatgagcattttctctacagtcagtctggtcagatcaccattgctgagctatcttagttctttggttatggcacctattgtgtggatcttttatatctcgctcacttctttatcactgtctatcttggggaagtccttcctacagccaaattatgttctgatccctgatgtttcaatatggtcagttgactttgtgaaatggtgggcactaaacaaagcacaagcattagctgcaaagatgctagctgtgcatctaaagggcacaattttcttaaattattggttcaagatgcaaggagctcggattggttcatctgttgtcatcgacacagtagacataaccgatccatccctgttgacggttgcagatggtgcagttcttgcagaaggcgctcttgttcagggccatgaagtctgtaatgaagtgctgagttttaggcctatctggattggctgtgaggcttcgattggtccttatgcggtgcttcagaagggaactgttgtggaagatggagctgttgttccaccattgcagaagactggggctgggaaatcaacacgtcggactagcagaacttctgtaagcataaaggtaactaaagtatgtagacttcataatctacttcggtgtaataaaactatgatttgttatggtatacaacgatagtttgttccttcctgaccagaaacgtatagttgattatttctagacattgaatatagtaagctacagacttataattacaccataggccatttgaatttccttgtggtctttaacatggatgaagctgccaccaaagaacttccctacctttttacatttagtgaaaatattttttgagattcaggaagggacatatattttatcagggaaccataagcaaatcatttatgtacaagtggtcaagcagaaaaatcatataaattaaacaagatgaagggtttgtttggattgaagtcaatcttttggcaacttcaatagtattgtatagatatttggtccgataccaaatttttcccacactagtgcaaattctactcttaacaaatttggtagtgccgaaatttaccaatgttttggcacttccagtattttggtaagacaaaaaaatggcttcaaaccaaactagcccaaactcttttctgcatggtgcatagtgtctgcgtgtgtgcattttcattcgacagtggggtatatatctaactgccttcaaagttctcgattgcttctgcagaacatgtttgttcatggccatatttatataatgttattcgaatacaaaccgagtattttttctttgcagaaagaagcagccaaagcaaacatgatacttgaacacctcgttagcatctacgcagttggcatcctgggtgctctgtccggtgcaatcgtctacacactatacactcacttgtccggcaaagcggcttcgcctctgcatttctcctttgcatgcattgctggtgcgttccactggttaccagctgccatcactgcctatgctgttattgtccaggagacacccacaagtgcactgtcatttgctctcttcactgccttcgccgacctgagctatggtgtcatcctcagtattctcaccagtataaccagcagagcccttgctgcaaagcctggaaccaagcagaatggcattgcgagtttgatccaccggcgcataaccatctctgctcatgtcaggtttgccaagatgctatctggaacagaagccttctgcgtgtacctgaggcttctcggagcaaagattggcaggcattgctctatccgagccatcaatccggtggcgaatcctgagctgatcagcgtcggtgatggcgtccatctaggtgacttctgcaatattgttccagggttctactccaaggggggattcaccagtgcagagattaaggtccaggagaacaccgtggttggcagtggcagcttgctcctccctggctgtgtcctccaggagaatgtcatcctcggcgcgctctccgtcgcgccggagaacgctgttctccggcgtggcggcgtctacgtcgggtcacagtctccggccatggtgaagaacaccttgcttgacgaggatgaacggatcgaagagatggatcaggcatacaagaagattgttggcaacctcgcggcgaacttggccatcaccaccatgaacgtcaagtcgaggtacttccaccgcatcggcgtgagcggccgcggcgtcctgaggatgtacgaggagataccgtcgtttccccggcacaagatcttcgcctccggcaagtccttcccggtgatcgtccgccacagcaacagcctgagcgccgacgacgacgcgaggctcgacgcccgcggcgccgcggtgcggatcctctcggacaacgacggcgaggcgcctctcctcgacctcacactcaagagcggcaaggcgttctacgcgcgcaccatcgccgacttcgccacctggctggtctgcggcctgccggcgagggaggagcaggtcaagcgctcgccgcacatccgcgacgccgtctggggctctctccggagcaccgactcgtacaccgtgctccactactactccaacatatgccggctgctgcggttcgacgacggcagggagatgtacgccaagttcaagctccgtcctgctgatccagacgtccccgaggactccggcaaggtggtgccgaggggcatcctgccgccggagaccggcgcgatccccagggacgaggatgacacgcgccctctgctcttcctcgccgacgacttccgccggagagtgggctcaccggacggggtgcgctacgtgttccagctgcagctcagggaggtgcccaccgacgccgccgcccgcgacgtcgccctcgactgcacccggccgtgggacgaggccgagttcccttacatcgacgtcggcgaggtcagcatcggccgcaacctcccgacggaggagacggagaagctcgagttcaacccgttcctccggtgccccgaggtggacgtcatcccggcgacgtcgtgcgcgcagagcgcgtccatcgaccacggccgctcgctggtctacgagatatgccagcgcctgcggaacggcgagccgctgcccgcgtcgtggcgagctttcctggagcagtcggacaccaagatcgacctgtcaggatgcccggtcgctgccgccgcgacacccacaaggtctaacgccggcgacgccacgaaggtgacgctggccaggacgtggtaccaggcgctctgggccacgctgtgccagccgctgctgcaaacgctggtgccgtactctgtcctgggcctcgtcatcttcctcccgctcaggggcctcctcgccgtcgccgcggccacccgcttcccgctctactggctcctcccggcgttctgggccgcgtcgggcgtcgccgccatggccacgtgcgcggcggcgaagtgggcgctggtcggcagcagggtcgacggcgacacggtgcacatctggtcgccggccgtgttcctggacacggtctggcaggccgtgcgcgccgccacggcggagtacttcgccgagctgacgcccgggtcggcgccgttcgcggcgtggatgcgggtgatgggcgcctccgtgtcccccggcgacggcgtgtacgtggactccatgggcgcgctgctgaacccggagatggtgcgcctggagcgcggcgccgccgtcgggcgggacgcgctgctgttcggccacgtgtacgagggcgaggccgggaaggtgaagttcggcgccgtgtccgtcggggaggacgggttcgtcggcagccgcgccgtggccatgcccagcgtcacggtggacgacggcggctgcctcgccgcgctcgggctggccatgaagggggagacggtgaagcacagcatgtaa&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001187049.1 RefSeq:Os04g0473900]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 4]]<br /> [[Category:Chromosome 4]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Dwa4.jpg&diff=175247 2014-06-01T01:34:15Z

<p>Mayamei0424: </p> <hr /> <div></div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Dwa3.jpg&diff=175246 2014-06-01T01:33:58Z

<p>Mayamei0424: </p> <hr /> <div></div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Dwa1.jpg&diff=175244 2014-06-01T01:33:37Z

<p>Mayamei0424: </p> <hr /> <div></div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Dwa2.jpg&diff=175243 2014-06-01T01:33:10Z

<p>Mayamei0424: </p> <hr /> <div></div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0257300&diff=174853 2014-05-31T03:31:11Z

<p>Mayamei0424: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''OsRAA1''(Oryza sativa Root Architecture Associated1) encodes a small protein (109 aa in length) conserved in plants <br /> including -GVWV/IF domain, -GWERYY domain ,-DLIS/ALP- and -H/YMYDI/VVV/I- domain. The OsRAA1 promoter(1,987 bp in length)includes elements of GA and auxin response, but it was a very weak response to GA3 treatment and it probably regulated by auxin. The OsRAA1 mRNA was specifically transcripted in the organs of roots and spikes, including root apex, cortex of root apical meristem ,pericycle of root apex, lateral roots, apical meristem of young spikes, collenchyma cells of margin vascular bundles between shoot and roots.In Ubi::OsRAA1 transgenic plants, overexpression ''OsRAA1'' in the root results in reduced growth of primary root, increased number of adventitious roots and helix primary root, and delayed gravitropic, which are similar to the phenotypes of the wild-type plant treated with auxin. Furthermore, the leaves and filaments are longer at the last stage of plant development in the transgenic plants. ''OsRAA1'' expression is induced by auxin. At the same time, overexpression of ''OsRAA1'' also causes endogenous indole-3-acetic acid to increase. These data suggest that ''OsRAA1'' as a new gene functions in the development of rice root systems,which are mediated by auxin.<br /> <br /> OsRAA1 Regulates Primary Root Development by Modulating Mitosis. OsRAA1 may function at the transition from metaphase to anaphase during cell division.OsRAA1 Interacts with OsRPT4 and Functions in Sister Chromatid Separation in Rice Root Cells.OsRAA1 was degraded by the 26S proteasome through interaction with OsRPT4,and APC perhaps serves to recognize OsRAA1 as E3.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> Immunoassay of a cell line with GFP alone showed immunofluorescence in the whole cell during the cell cycle.In contrast, in the OsRAA1-GFP transgenic line, OsRAA1 GFP was enriched at spindles, including tubulins, from metaphase to anaphase during cell division<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> *OsRAA1 Interaction Protein Screening<br /> *OsRAA1 Interacts with OsRPT4 in Vitro and in Vivo<br /> *Aberrant Mitosis Occurs in OsRAA1 Transgenic Rice and Transgenic Yeast Cells<br /> *Degradation of OsRAA1 Depends on the Proteasome<br /> *Two-Hybrid cDNA Library Construction and Screening<br /> *Protein Deletion Mutation and Yeast Plasmid Construction<br /> *Pull-Down Assay<br /> *Immunofluorescence of Rice Root Tips<br /> *Coimmunoprecipitation and Tris-Tricine Gel Electrophoresis<br /> *Colocalization of OsRAA1 and Tubulin in the BY-2 Cell Line<br /> *Transient Subcellular Localization<br /> *Determination of the Half-Life of OsRAA1<br /> *Protein Degradation Assay in Vitro<br /> *Morphological Examination of Transformed S. pombeCells<br /> *Research Center for Molecular and Developmental Biology, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences,Beijing 100093, China<br /> *State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100094,China<br /> *Department Plant Biology, Carnegie Institution of Washington, and Department of Biological Sciences, Stanford University, Stanford, California 94305<br /> *National Research Center for Plant Gene, Beijing 100093, China<br /> *Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> ==References==<br /> *Rice ROOT ARCHITECTURE ASSOCIATED1 Binds the Proteasome Subunit RPT4 and Is Degraded in a D-Box and Proteasome-Dependent Manner Plant Physiology, 2008, 148(2): 843-855<br /> *Overexpression of OsRAA1 Causes Pleiotropic Phenotypes in Transgenic Rice Plants, including Altered Leaf, Flower, and Root Development and Root Response to Gravity Plant Physiology, 2004, 135(3): 1502-1513<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0257300|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001049166.1 GI:115435745 GeneID:4326229|<br /> Length = 785 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0257300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:8585137..8585921|<br /> CDS = 8585497..8585826|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MSGVWVFKNGVVRLVEKQQATAGTAVAGGRRKALVHTPSGQVVS SYAALEARLTALGWERYYEDPSLFQFHKRGSLDLISLPADFSAFSSVHMYDIVVKNRD SFRVVDA&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;96..425#tccatccacccatagctatctctagctagcttgcacattcttcattcttcttgcaatcagagctagagaaaaagagtttgagagagatctaagagatgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaaattgacctatgtataggctcgcatgcatgcaaggtagacgaccatccaccttgcatgcatgcaggctatcattctctcttcgtctccgtcttcgtctttcatctttgttgtggtgtgtgtgaatttattatacttcttatgactgtgtgtgtgactgtgtgagagttcatggagagtgatgagtagtggtatacatatgtgtcgtcgctgatcgatttggtttattaccttgtgggtgggtattaattatcagcagtgtgttatatcaattgcttacttgtatgtgtatcatgtacatgagtgagtgtggctgatgcatatatatagaggtctttaataattatgtactatatatttgtg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049166.1 RefSeq:Os01g0257300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0257300&diff=174851 2014-05-31T03:25:18Z

<p>Mayamei0424: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''OsRAA1''(Oryza sativa Root Architecture Associated1) encodes a small protein (109 aa in length) conserved in plants <br /> including -GVWV/IF domain, -GWERYY domain ,-DLIS/ALP- and -H/YMYDI/VVV/I- domain. The OsRAA1 promoter(1,987 bp in length)includes elements of GA and auxin response, but it was a very weak response to GA3 treatment and it probably regulated by auxin. The OsRAA1 mRNA was specifically transcripted in the organs of roots and spikes, including root apex, cortex of root apical meristem ,pericycle of root apex, lateral roots, apical meristem of young spikes, collenchyma cells of margin vascular bundles between shoot and roots.In Ubi::OsRAA1 transgenic plants, overexpression ''OsRAA1'' in the root results in reduced growth of primary root, increased number of adventitious roots and helix primary root, and delayed gravitropic, which are similar to the phenotypes of the wild-type plant treated with auxin. Furthermore, the leaves and filaments are longer at the last stage of plant development in the transgenic plants. ''OsRAA1'' expression is induced by auxin. At the same time, overexpression of ''OsRAA1'' also causes endogenous indole-3-acetic acid to increase. These data suggest that ''OsRAA1'' as a new gene functions in the development of rice root systems,which are mediated by auxin.<br /> <br /> OsRAA1 Regulates Primary Root Development by Modulating Mitosis. OsRAA1 may function at the transition from metaphase to anaphase during cell division.OsRAA1 Interacts with OsRPT4 and Functions in Sister Chromatid Separation in Rice Root Cells.OsRAA1 was degraded by the 26S proteasome through interaction with OsRPT4,and APC perhaps serves to recognize OsRAA1 as E3.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> Immunoassay of a cell line with GFP alone showed immunofluorescence in the whole cell during the cell cycle.In contrast, in the OsRAA1-GFP transgenic line, OsRAA1 GFP was enriched at spindles, including tubulins, from metaphase to anaphase during cell division<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> *OsRAA1 Interaction Protein Screening<br /> *OsRAA1 Interacts with OsRPT4 in Vitro and in Vivo<br /> *Aberrant Mitosis Occurs in OsRAA1 Transgenic Rice and Transgenic Yeast Cells<br /> *Degradation of OsRAA1 Depends on the Proteasome<br /> *Two-Hybrid cDNA Library Construction and Screening<br /> *Protein Deletion Mutation and Yeast Plasmid Construction<br /> *Pull-Down Assay<br /> *Immunofluorescence of Rice Root Tips<br /> *Coimmunoprecipitation and Tris-Tricine Gel Electrophoresis<br /> *Colocalization of OsRAA1 and Tubulin in the BY-2 Cell Line<br /> *Transient Subcellular Localization<br /> *Determination of the Half-Life of OsRAA1<br /> *Protein Degradation Assay in Vitro<br /> *Morphological Examination of Transformed S. pombeCells<br /> <br /> ==References==<br /> *Rice ROOT ARCHITECTURE ASSOCIATED1 Binds the Proteasome Subunit RPT4 and Is Degraded in a D-Box and Proteasome-Dependent Manner Plant Physiology, 2008, 148(2): 843-855<br /> *Overexpression of OsRAA1 Causes Pleiotropic Phenotypes in Transgenic Rice Plants, including Altered Leaf, Flower, and Root Development and Root Response to Gravity Plant Physiology, 2004, 135(3): 1502-1513<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0257300|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001049166.1 GI:115435745 GeneID:4326229|<br /> Length = 785 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0257300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:8585137..8585921|<br /> CDS = 8585497..8585826|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MSGVWVFKNGVVRLVEKQQATAGTAVAGGRRKALVHTPSGQVVS SYAALEARLTALGWERYYEDPSLFQFHKRGSLDLISLPADFSAFSSVHMYDIVVKNRD SFRVVDA&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;96..425#tccatccacccatagctatctctagctagcttgcacattcttcattcttcttgcaatcagagctagagaaaaagagtttgagagagatctaagagatgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaaattgacctatgtataggctcgcatgcatgcaaggtagacgaccatccaccttgcatgcatgcaggctatcattctctcttcgtctccgtcttcgtctttcatctttgttgtggtgtgtgtgaatttattatacttcttatgactgtgtgtgtgactgtgtgagagttcatggagagtgatgagtagtggtatacatatgtgtcgtcgctgatcgatttggtttattaccttgtgggtgggtattaattatcagcagtgtgttatatcaattgcttacttgtatgtgtatcatgtacatgagtgagtgtggctgatgcatatatatagaggtctttaataattatgtactatatatttgtg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049166.1 RefSeq:Os01g0257300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0257300&diff=174850 2014-05-31T03:24:59Z

<p>Mayamei0424: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> ''OsRAA1''(Oryza sativa Root Architecture Associated1) encodes a small protein (109 aa in length) conserved in plants <br /> ''OsRAA1''(Oryza sativa Root Architecture Associated1) encodes a small protein (109 aa in length) conserved in plants <br /> including -GVWV/IF domain, -GWERYY domain ,-DLIS/ALP- and -H/YMYDI/VVV/I- domain. The OsRAA1 promoter(1,987 bp in length)includes elements of GA and auxin response, but it was a very weak response to GA3 treatment and it probably regulated by auxin. The OsRAA1 mRNA was specifically transcripted in the organs of roots and spikes, including root apex, cortex of root apical meristem ,pericycle of root apex, lateral roots, apical meristem of young spikes, collenchyma cells of margin vascular bundles between shoot and roots.In Ubi::OsRAA1 transgenic plants, overexpression ''OsRAA1'' in the root results in reduced growth of primary root, increased number of adventitious roots and helix primary root, and delayed gravitropic, which are similar to the phenotypes of the wild-type plant treated with auxin. Furthermore, the leaves and filaments are longer at the last stage of plant development in the transgenic plants. ''OsRAA1'' expression is induced by auxin. At the same time, overexpression of ''OsRAA1'' also causes endogenous indole-3-acetic acid to increase. These data suggest that ''OsRAA1'' as a new gene functions in the development of rice root systems,which are mediated by auxin.<br /> <br /> OsRAA1 Regulates Primary Root Development by Modulating Mitosis. OsRAA1 may function at the transition from metaphase to anaphase during cell division.OsRAA1 Interacts with OsRPT4 and Functions in Sister Chromatid Separation in Rice Root Cells.OsRAA1 was degraded by the 26S proteasome through interaction with OsRPT4,and APC perhaps serves to recognize OsRAA1 as E3.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> Immunoassay of a cell line with GFP alone showed immunofluorescence in the whole cell during the cell cycle.In contrast, in the OsRAA1-GFP transgenic line, OsRAA1 GFP was enriched at spindles, including tubulins, from metaphase to anaphase during cell division<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> *OsRAA1 Interaction Protein Screening<br /> *OsRAA1 Interacts with OsRPT4 in Vitro and in Vivo<br /> *Aberrant Mitosis Occurs in OsRAA1 Transgenic Rice and Transgenic Yeast Cells<br /> *Degradation of OsRAA1 Depends on the Proteasome<br /> *Two-Hybrid cDNA Library Construction and Screening<br /> *Protein Deletion Mutation and Yeast Plasmid Construction<br /> *Pull-Down Assay<br /> *Immunofluorescence of Rice Root Tips<br /> *Coimmunoprecipitation and Tris-Tricine Gel Electrophoresis<br /> *Colocalization of OsRAA1 and Tubulin in the BY-2 Cell Line<br /> *Transient Subcellular Localization<br /> *Determination of the Half-Life of OsRAA1<br /> *Protein Degradation Assay in Vitro<br /> *Morphological Examination of Transformed S. pombeCells<br /> <br /> ==References==<br /> *Rice ROOT ARCHITECTURE ASSOCIATED1 Binds the Proteasome Subunit RPT4 and Is Degraded in a D-Box and Proteasome-Dependent Manner Plant Physiology, 2008, 148(2): 843-855<br /> *Overexpression of OsRAA1 Causes Pleiotropic Phenotypes in Transgenic Rice Plants, including Altered Leaf, Flower, and Root Development and Root Response to Gravity Plant Physiology, 2004, 135(3): 1502-1513<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0257300|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001049166.1 GI:115435745 GeneID:4326229|<br /> Length = 785 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0257300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:8585137..8585921|<br /> CDS = 8585497..8585826|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MSGVWVFKNGVVRLVEKQQATAGTAVAGGRRKALVHTPSGQVVS SYAALEARLTALGWERYYEDPSLFQFHKRGSLDLISLPADFSAFSSVHMYDIVVKNRD SFRVVDA&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;96..425#tccatccacccatagctatctctagctagcttgcacattcttcattcttcttgcaatcagagctagagaaaaagagtttgagagagatctaagagatgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaaattgacctatgtataggctcgcatgcatgcaaggtagacgaccatccaccttgcatgcatgcaggctatcattctctcttcgtctccgtcttcgtctttcatctttgttgtggtgtgtgtgaatttattatacttcttatgactgtgtgtgtgactgtgtgagagttcatggagagtgatgagtagtggtatacatatgtgtcgtcgctgatcgatttggtttattaccttgtgggtgggtattaattatcagcagtgtgttatatcaattgcttacttgtatgtgtatcatgtacatgagtgagtgtggctgatgcatatatatagaggtctttaataattatgtactatatatttgtg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049166.1 RefSeq:Os01g0257300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0257300&diff=174849 2014-05-31T03:24:14Z

<p>Mayamei0424: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> OsRAA1(Oryza sativa Root Architecture Associated1) encodes a small protein (109 aa in length) conserved in plants including -GVWV/IF domain, <br /> -GWERYY domain ,-DLIS/ALP- and -H/YMYDI/VVV/I- domain. The OsRAA1 promoter(1,987 bp in length)includes elements of GA and auxin response, but it was a very weak response to GA3 treatment and it probably regulated by auxin. The OsRAA1 mRNA was specifically transcripted in the organs of roots and spikes, including root apex, cortex of root apical meristem ,pericycle of root apex, lateral roots, apical meristem of young spikes, collenchyma cells of margin vascular bundles between shoot and roots.In Ubi::OsRAA1 transgenic plants, overexpression ''OsRAA1'' in the root results in reduced growth of primary root, increased number of adventitious roots and helix primary root, and delayed gravitropic, which are similar to the phenotypes of the wild-type plant treated with auxin. Furthermore, the leaves and filaments are longer at the last stage of plant development in the transgenic plants. ''OsRAA1'' expression is induced by auxin. At the same time, overexpression of ''OsRAA1'' also causes endogenous indole-3-acetic acid to increase. These data suggest that ''OsRAA1'' as a new gene functions in the development of rice root systems,which are mediated by auxin.<br /> <br /> OsRAA1 Regulates Primary Root Development by Modulating Mitosis. OsRAA1 may function at the transition from metaphase to anaphase during cell division.OsRAA1 Interacts with OsRPT4 and Functions in Sister Chromatid Separation in Rice Root Cells.OsRAA1 was degraded by the 26S proteasome through interaction with OsRPT4,and APC perhaps serves to recognize OsRAA1 as E3.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> Immunoassay of a cell line with GFP alone showed immunofluorescence in the whole cell during the cell cycle.In contrast, in the OsRAA1-GFP transgenic line, OsRAA1 GFP was enriched at spindles, including tubulins, from metaphase to anaphase during cell division<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> *OsRAA1 Interaction Protein Screening<br /> *OsRAA1 Interacts with OsRPT4 in Vitro and in Vivo<br /> *Aberrant Mitosis Occurs in OsRAA1 Transgenic Rice and Transgenic Yeast Cells<br /> *Degradation of OsRAA1 Depends on the Proteasome<br /> *Two-Hybrid cDNA Library Construction and Screening<br /> *Protein Deletion Mutation and Yeast Plasmid Construction<br /> *Pull-Down Assay<br /> *Immunofluorescence of Rice Root Tips<br /> *Coimmunoprecipitation and Tris-Tricine Gel Electrophoresis<br /> *Colocalization of OsRAA1 and Tubulin in the BY-2 Cell Line<br /> *Transient Subcellular Localization<br /> *Determination of the Half-Life of OsRAA1<br /> *Protein Degradation Assay in Vitro<br /> *Morphological Examination of Transformed S. pombeCells<br /> <br /> ==References==<br /> *Rice ROOT ARCHITECTURE ASSOCIATED1 Binds the Proteasome Subunit RPT4 and Is Degraded in a D-Box and Proteasome-Dependent Manner Plant Physiology, 2008, 148(2): 843-855<br /> *Overexpression of OsRAA1 Causes Pleiotropic Phenotypes in Transgenic Rice Plants, including Altered Leaf, Flower, and Root Development and Root Response to Gravity Plant Physiology, 2004, 135(3): 1502-1513<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0257300|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001049166.1 GI:115435745 GeneID:4326229|<br /> Length = 785 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0257300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:8585137..8585921|<br /> CDS = 8585497..8585826|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MSGVWVFKNGVVRLVEKQQATAGTAVAGGRRKALVHTPSGQVVS SYAALEARLTALGWERYYEDPSLFQFHKRGSLDLISLPADFSAFSSVHMYDIVVKNRD SFRVVDA&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;96..425#tccatccacccatagctatctctagctagcttgcacattcttcattcttcttgcaatcagagctagagaaaaagagtttgagagagatctaagagatgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaaattgacctatgtataggctcgcatgcatgcaaggtagacgaccatccaccttgcatgcatgcaggctatcattctctcttcgtctccgtcttcgtctttcatctttgttgtggtgtgtgtgaatttattatacttcttatgactgtgtgtgtgactgtgtgagagttcatggagagtgatgagtagtggtatacatatgtgtcgtcgctgatcgatttggtttattaccttgtgggtgggtattaattatcagcagtgtgttatatcaattgcttacttgtatgtgtatcatgtacatgagtgagtgtggctgatgcatatatatagaggtctttaataattatgtactatatatttgtg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049166.1 RefSeq:Os01g0257300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0257300&diff=174848 2014-05-31T03:23:38Z

<p>Mayamei0424: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> ''OsRAA1'' (Oryza sativa Root Architecture Associated1) encodes a small protein (109 aa in length) conserved in plants including -GVWV/IF domain, <br /> -GWERYY domain ,-DLIS/ALP- and -H/YMYDI/VVV/I- domain. <br /> The OsRAA1 promoter(1,987 bp in length)includes elements of GA and auxin response, but it was a very weak response to GA3 treatment and it probably regulated by auxin. <br /> The OsRAA1 mRNA was specifically transcripted in the organs of roots and spikes, including root apex, cortex of root apical meristem ,pericycle of root apex, lateral roots, apical meristem of young spikes, collenchyma cells of margin vascular bundles between shoot and roots.In Ubi::OsRAA1 transgenic plants, overexpression ''OsRAA1'' in the root results in reduced growth of primary root, increased number of adventitious roots and helix primary root, and delayed gravitropic, which are similar to the phenotypes of the wild-type plant treated with auxin. Furthermore, the leaves and filaments are longer at the last stage of plant development in the transgenic plants. ''OsRAA1'' expression is induced by auxin. At the same time, overexpression of ''OsRAA1'' also causes endogenous indole-3-acetic acid to increase. These data suggest that ''OsRAA1'' as a new gene functions in the development of rice root systems,which are mediated by auxin.<br /> <br /> OsRAA1 Regulates Primary Root Development by Modulating Mitosis. OsRAA1 may function at the transition from metaphase to anaphase during cell division.OsRAA1 Interacts with OsRPT4 and Functions in Sister Chromatid Separation in Rice Root Cells.OsRAA1 was degraded by the 26S proteasome through interaction with OsRPT4,and APC perhaps serves to recognize OsRAA1 as E3.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> Immunoassay of a cell line with GFP alone showed immunofluorescence in the whole cell during the cell cycle.In contrast, in the OsRAA1-GFP transgenic line, OsRAA1 GFP was enriched at spindles, including tubulins, from metaphase to anaphase during cell division<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> *OsRAA1 Interaction Protein Screening<br /> *OsRAA1 Interacts with OsRPT4 in Vitro and in Vivo<br /> *Aberrant Mitosis Occurs in OsRAA1 Transgenic Rice and Transgenic Yeast Cells<br /> *Degradation of OsRAA1 Depends on the Proteasome<br /> *Two-Hybrid cDNA Library Construction and Screening<br /> *Protein Deletion Mutation and Yeast Plasmid Construction<br /> *Pull-Down Assay<br /> *Immunofluorescence of Rice Root Tips<br /> *Coimmunoprecipitation and Tris-Tricine Gel Electrophoresis<br /> *Colocalization of OsRAA1 and Tubulin in the BY-2 Cell Line<br /> *Transient Subcellular Localization<br /> *Determination of the Half-Life of OsRAA1<br /> *Protein Degradation Assay in Vitro<br /> *Morphological Examination of Transformed S. pombeCells<br /> <br /> ==References==<br /> *Rice ROOT ARCHITECTURE ASSOCIATED1 Binds the Proteasome Subunit RPT4 and Is Degraded in a D-Box and Proteasome-Dependent Manner Plant Physiology, 2008, 148(2): 843-855<br /> *Overexpression of OsRAA1 Causes Pleiotropic Phenotypes in Transgenic Rice Plants, including Altered Leaf, Flower, and Root Development and Root Response to Gravity Plant Physiology, 2004, 135(3): 1502-1513<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0257300|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001049166.1 GI:115435745 GeneID:4326229|<br /> Length = 785 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0257300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:8585137..8585921|<br /> CDS = 8585497..8585826|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MSGVWVFKNGVVRLVEKQQATAGTAVAGGRRKALVHTPSGQVVS SYAALEARLTALGWERYYEDPSLFQFHKRGSLDLISLPADFSAFSSVHMYDIVVKNRD SFRVVDA&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;96..425#tccatccacccatagctatctctagctagcttgcacattcttcattcttcttgcaatcagagctagagaaaaagagtttgagagagatctaagagatgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaaattgacctatgtataggctcgcatgcatgcaaggtagacgaccatccaccttgcatgcatgcaggctatcattctctcttcgtctccgtcttcgtctttcatctttgttgtggtgtgtgtgaatttattatacttcttatgactgtgtgtgtgactgtgtgagagttcatggagagtgatgagtagtggtatacatatgtgtcgtcgctgatcgatttggtttattaccttgtgggtgggtattaattatcagcagtgtgttatatcaattgcttacttgtatgtgtatcatgtacatgagtgagtgtggctgatgcatatatatagaggtctttaataattatgtactatatatttgtg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049166.1 RefSeq:Os01g0257300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0257300&diff=174847 2014-05-31T03:23:23Z

<p>Mayamei0424: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''OsRAA1'' (Oryza sativa Root Architecture Associated1) encodes a small protein (109 aa in length) conserved in plants including -GVWV/IF domain, <br /> -GWERYY domain ,-DLIS/ALP- and -H/YMYDI/VVV/I- domain. <br /> The OsRAA1 promoter(1,987 bp in length)includes elements of GA and auxin response, but it was a very weak response to GA3 treatment and it probably regulated by auxin. <br /> The OsRAA1 mRNA was specifically transcripted in the organs of roots and spikes, including root apex, cortex of root apical meristem ,pericycle of root apex, lateral roots, apical meristem of young spikes, collenchyma cells of margin vascular bundles between shoot and roots.In Ubi::OsRAA1 transgenic plants, overexpression ''OsRAA1'' in the root results in reduced growth of primary root, increased number of adventitious roots and helix primary root, and delayed gravitropic, which are similar to the phenotypes of the wild-type plant treated with auxin. Furthermore, the leaves and filaments are longer at the last stage of plant development in the transgenic plants. ''OsRAA1'' expression is induced by auxin. At the same time, overexpression of ''OsRAA1'' also causes endogenous indole-3-acetic acid to increase. These data suggest that ''OsRAA1'' as a new gene functions in the development of rice root systems,which are mediated by auxin.<br /> <br /> OsRAA1 Regulates Primary Root Development by Modulating Mitosis. OsRAA1 may function at the transition from metaphase to anaphase during cell division.OsRAA1 Interacts with OsRPT4 and Functions in Sister Chromatid Separation in Rice Root Cells.OsRAA1 was degraded by the 26S proteasome through interaction with OsRPT4,and APC perhaps serves to recognize OsRAA1 as E3.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> Immunoassay of a cell line with GFP alone showed immunofluorescence in the whole cell during the cell cycle.In contrast, in the OsRAA1-GFP transgenic line, OsRAA1 GFP was enriched at spindles, including tubulins, from metaphase to anaphase during cell division<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> *OsRAA1 Interaction Protein Screening<br /> *OsRAA1 Interacts with OsRPT4 in Vitro and in Vivo<br /> *Aberrant Mitosis Occurs in OsRAA1 Transgenic Rice and Transgenic Yeast Cells<br /> *Degradation of OsRAA1 Depends on the Proteasome<br /> *Two-Hybrid cDNA Library Construction and Screening<br /> *Protein Deletion Mutation and Yeast Plasmid Construction<br /> *Pull-Down Assay<br /> *Immunofluorescence of Rice Root Tips<br /> *Coimmunoprecipitation and Tris-Tricine Gel Electrophoresis<br /> *Colocalization of OsRAA1 and Tubulin in the BY-2 Cell Line<br /> *Transient Subcellular Localization<br /> *Determination of the Half-Life of OsRAA1<br /> *Protein Degradation Assay in Vitro<br /> *Morphological Examination of Transformed S. pombeCells<br /> <br /> ==References==<br /> *Rice ROOT ARCHITECTURE ASSOCIATED1 Binds the Proteasome Subunit RPT4 and Is Degraded in a D-Box and Proteasome-Dependent Manner Plant Physiology, 2008, 148(2): 843-855<br /> *Overexpression of OsRAA1 Causes Pleiotropic Phenotypes in Transgenic Rice Plants, including Altered Leaf, Flower, and Root Development and Root Response to Gravity Plant Physiology, 2004, 135(3): 1502-1513<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0257300|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001049166.1 GI:115435745 GeneID:4326229|<br /> Length = 785 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0257300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:8585137..8585921|<br /> CDS = 8585497..8585826|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MSGVWVFKNGVVRLVEKQQATAGTAVAGGRRKALVHTPSGQVVS SYAALEARLTALGWERYYEDPSLFQFHKRGSLDLISLPADFSAFSSVHMYDIVVKNRD SFRVVDA&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;96..425#tccatccacccatagctatctctagctagcttgcacattcttcattcttcttgcaatcagagctagagaaaaagagtttgagagagatctaagagatgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaaattgacctatgtataggctcgcatgcatgcaaggtagacgaccatccaccttgcatgcatgcaggctatcattctctcttcgtctccgtcttcgtctttcatctttgttgtggtgtgtgtgaatttattatacttcttatgactgtgtgtgtgactgtgtgagagttcatggagagtgatgagtagtggtatacatatgtgtcgtcgctgatcgatttggtttattaccttgtgggtgggtattaattatcagcagtgtgttatatcaattgcttacttgtatgtgtatcatgtacatgagtgagtgtggctgatgcatatatatagaggtctttaataattatgtactatatatttgtg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049166.1 RefSeq:Os01g0257300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0257300&diff=174845 2014-05-31T03:22:40Z

<p>Mayamei0424: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''OsRAA1'' (Oryza sativa Root Architecture Associated1) encodes a small protein (109 aa in length) conserved in plants including -GVWV/IF domain, -GWERYY domain ,-DLIS/ALP- and -H/YMYDI/VVV/I- domain. The OsRAA1 promoter(1,987 bp in length)includes elements of GA and auxin response, but it was a very weak response to GA3 treatment and it probably regulated by auxin. The OsRAA1 mRNA was specifically transcripted in the organs of roots and spikes, including root apex, cortex of root apical meristem ,pericycle of root apex, lateral roots, apical meristem of young spikes, collenchyma cells of margin vascular bundles between shoot and roots.In Ubi::OsRAA1 transgenic plants, overexpression ''OsRAA1'' in the root results in reduced growth of primary root, increased number of adventitious roots and helix primary root, and delayed gravitropic, which are similar to the phenotypes of the wild-type plant treated with auxin. Furthermore, the leaves and filaments are longer at the last stage of plant development in the transgenic plants. ''OsRAA1'' expression is induced by auxin. At the same time, overexpression of ''OsRAA1'' also causes endogenous indole-3-acetic acid to increase. These data suggest that ''OsRAA1'' as a new gene functions in the development of rice root systems,which are mediated by auxin.OsRAA1 Regulates Primary Root Development by Modulating Mitosis. OsRAA1 may function at the transition from metaphase to anaphase during cell division.OsRAA1 Interacts with OsRPT4 and Functions in Sister Chromatid Separation in Rice Root Cells.OsRAA1 was degraded by the 26S proteasome through interaction with OsRPT4,and APC perhaps serves to recognize OsRAA1 as E3.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> Immunoassay of a cell line with GFP alone showed immunofluorescence in the whole cell during the cell cycle.In contrast, in the OsRAA1-GFP transgenic line, OsRAA1 GFP was enriched at spindles, including tubulins, from metaphase to anaphase during cell division<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> *OsRAA1 Interaction Protein Screening<br /> *OsRAA1 Interacts with OsRPT4 in Vitro and in Vivo<br /> *Aberrant Mitosis Occurs in OsRAA1 Transgenic Rice and Transgenic Yeast Cells<br /> *Degradation of OsRAA1 Depends on the Proteasome<br /> *Two-Hybrid cDNA Library Construction and Screening<br /> *Protein Deletion Mutation and Yeast Plasmid Construction<br /> *Pull-Down Assay<br /> *Immunofluorescence of Rice Root Tips<br /> *Coimmunoprecipitation and Tris-Tricine Gel Electrophoresis<br /> *Colocalization of OsRAA1 and Tubulin in the BY-2 Cell Line<br /> *Transient Subcellular Localization<br /> *Determination of the Half-Life of OsRAA1<br /> *Protein Degradation Assay in Vitro<br /> *Morphological Examination of Transformed S. pombeCells<br /> <br /> ==References==<br /> *Rice ROOT ARCHITECTURE ASSOCIATED1 Binds the Proteasome Subunit RPT4 and Is Degraded in a D-Box and Proteasome-Dependent Manner Plant Physiology, 2008, 148(2): 843-855<br /> *Overexpression of OsRAA1 Causes Pleiotropic Phenotypes in Transgenic Rice Plants, including Altered Leaf, Flower, and Root Development and Root Response to Gravity Plant Physiology, 2004, 135(3): 1502-1513<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0257300|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001049166.1 GI:115435745 GeneID:4326229|<br /> Length = 785 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0257300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:8585137..8585921|<br /> CDS = 8585497..8585826|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MSGVWVFKNGVVRLVEKQQATAGTAVAGGRRKALVHTPSGQVVS SYAALEARLTALGWERYYEDPSLFQFHKRGSLDLISLPADFSAFSSVHMYDIVVKNRD SFRVVDA&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;96..425#tccatccacccatagctatctctagctagcttgcacattcttcattcttcttgcaatcagagctagagaaaaagagtttgagagagatctaagagatgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaaattgacctatgtataggctcgcatgcatgcaaggtagacgaccatccaccttgcatgcatgcaggctatcattctctcttcgtctccgtcttcgtctttcatctttgttgtggtgtgtgtgaatttattatacttcttatgactgtgtgtgtgactgtgtgagagttcatggagagtgatgagtagtggtatacatatgtgtcgtcgctgatcgatttggtttattaccttgtgggtgggtattaattatcagcagtgtgttatatcaattgcttacttgtatgtgtatcatgtacatgagtgagtgtggctgatgcatatatatagaggtctttaataattatgtactatatatttgtg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049166.1 RefSeq:Os01g0257300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0257300&diff=174840 2014-05-31T03:18:10Z

<p>Mayamei0424: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''OsRAA1'' (Oryza sativa Root Architecture Associated1) encodes a small protein (109 aa in length) conserved in plants including -GVWV/IF domain, -GWERYY domain ,-DLIS/ALP- and -H/YMYDI/VVV/I- domain. The OsRAA1 promoter(1,987 bp in length)includes elements of GA and auxin response, but it was a very weak response to GA3 treatment and it probably regulated by auxin. The OsRAA1 mRNA was specifically transcripted in the organs of roots and spikes, including root apex, cortex of root apical meristem ,pericycle of root apex, lateral roots, apical meristem of young spikes, collenchyma cells of margin vascular bundles between shoot and roots.In Ubi::OsRAA1 transgenic plants, overexpression ''OsRAA1'' in the root results in reduced growth of primary root, increased number of adventitious roots and helix primary root, and delayed gravitropic, which are similar to the phenotypes of the wild-type plant treated with auxin. Furthermore, the leaves and filaments are longer at the last stage of plant development in the transgenic plants. ''OsRAA1'' expression is induced by auxin. At the same time, overexpression of ''OsRAA1'' also causes endogenous indole-3-acetic acid to increase. These data suggest that ''OsRAA1'' as a new gene functions in the development of rice root systems,which are mediated by auxin.<br /> <br /> OsRAA1 Regulates Primary Root Development by Modulating Mitosis. OsRAA1 may function at the transition from metaphase to anaphase during cell division.OsRAA1 Interacts with OsRPT4 and Functions in Sister Chromatid Separation in Rice Root Cells.OsRAA1 was degraded by the 26S proteasome through interaction with OsRPT4,and APC perhaps serves to recognize OsRAA1 as E3.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> Immunoassay of a cell line with GFP alone showed immunofluorescence in the whole cell during the cell cycle.In contrast, in the OsRAA1-GFP transgenic line, OsRAA1 GFP was enriched at spindles, including tubulins, from metaphase to anaphase during cell division<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> *OsRAA1 Interaction Protein Screening<br /> *OsRAA1 Interacts with OsRPT4 in Vitro and in Vivo<br /> *Aberrant Mitosis Occurs in OsRAA1 Transgenic Rice and Transgenic Yeast Cells<br /> *Degradation of OsRAA1 Depends on the Proteasome<br /> *Two-Hybrid cDNA Library Construction and Screening<br /> *Protein Deletion Mutation and Yeast Plasmid Construction<br /> *Pull-Down Assay<br /> *Immunofluorescence of Rice Root Tips<br /> *Coimmunoprecipitation and Tris-Tricine Gel Electrophoresis<br /> *Colocalization of OsRAA1 and Tubulin in the BY-2 Cell Line<br /> *Transient Subcellular Localization<br /> *Determination of the Half-Life of OsRAA1<br /> *Protein Degradation Assay in Vitro<br /> *Morphological Examination of Transformed S. pombeCells<br /> <br /> ==References==<br /> *Rice ROOT ARCHITECTURE ASSOCIATED1 Binds the Proteasome Subunit RPT4 and Is Degraded in a D-Box and Proteasome-Dependent Manner Plant Physiology, 2008, 148(2): 843-855<br /> *Overexpression of OsRAA1 Causes Pleiotropic Phenotypes in Transgenic Rice Plants, including Altered Leaf, Flower, and Root Development and Root Response to Gravity Plant Physiology, 2004, 135(3): 1502-1513<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0257300|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001049166.1 GI:115435745 GeneID:4326229|<br /> Length = 785 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0257300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:8585137..8585921|<br /> CDS = 8585497..8585826|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MSGVWVFKNGVVRLVEKQQATAGTAVAGGRRKALVHTPSGQVVS SYAALEARLTALGWERYYEDPSLFQFHKRGSLDLISLPADFSAFSSVHMYDIVVKNRD SFRVVDA&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;96..425#tccatccacccatagctatctctagctagcttgcacattcttcattcttcttgcaatcagagctagagaaaaagagtttgagagagatctaagagatgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaaattgacctatgtataggctcgcatgcatgcaaggtagacgaccatccaccttgcatgcatgcaggctatcattctctcttcgtctccgtcttcgtctttcatctttgttgtggtgtgtgtgaatttattatacttcttatgactgtgtgtgtgactgtgtgagagttcatggagagtgatgagtagtggtatacatatgtgtcgtcgctgatcgatttggtttattaccttgtgggtgggtattaattatcagcagtgtgttatatcaattgcttacttgtatgtgtatcatgtacatgagtgagtgtggctgatgcatatatatagaggtctttaataattatgtactatatatttgtg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049166.1 RefSeq:Os01g0257300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0257300&diff=174839 2014-05-31T03:17:49Z

<p>Mayamei0424: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''OsRAA1'' (Oryza sativa Root Architecture Associated1) encodes a small protein (109 aa in length) conserved in plants including -GVWV/IF domain, -GWERYY domain ,-DLIS/ALP- and -H/YMYDI/VVV/I- domain. The OsRAA1 promoter(1,987 bp in length)includes elements of GA and auxin response, but it was a very weak response to GA3 treatment and it probably regulated by auxin. The OsRAA1 mRNA was specifically transcripted in the organs of roots and spikes, including root apex, cortex of root apical meristem ,pericycle of root apex, lateral roots, apical meristem of young spikes, collenchyma cells of margin vascular bundles between shoot and roots.In Ubi::OsRAA1 transgenic plants, overexpression ''OsRAA1'' in the root results in reduced growth of primary root, increased number of adventitious roots and helix primary root, and delayed gravitropic, which are similar to the phenotypes of the wild-type plant treated with auxin. Furthermore, the leaves and filaments are longer at the last stage of plant development in the transgenic plants. ''OsRAA1'' expression is induced by auxin. At the same time, overexpression of ''OsRAA1'' also causes endogenous indole-3-acetic acid to increase. These data suggest that ''OsRAA1'' as a new gene functions in the development of rice root systems,which are mediated by auxin&lt;ref name=&quot;ref1&quot;/&gt;.<br /> <br /> OsRAA1 Regulates Primary Root Development by Modulating Mitosis. OsRAA1 may function at the transition from metaphase to anaphase during cell division.OsRAA1 Interacts with OsRPT4 and Functions in Sister Chromatid Separation in Rice Root Cells.OsRAA1 was degraded by the 26S proteasome through interaction with OsRPT4,and APC perhaps serves to recognize OsRAA1 as E3.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> Immunoassay of a cell line with GFP alone showed immunofluorescence in the whole cell during the cell cycle.In contrast, in the OsRAA1-GFP transgenic line, OsRAA1 GFP was enriched at spindles, including tubulins, from metaphase to anaphase during cell division<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> *OsRAA1 Interaction Protein Screening<br /> *OsRAA1 Interacts with OsRPT4 in Vitro and in Vivo<br /> *Aberrant Mitosis Occurs in OsRAA1 Transgenic Rice and Transgenic Yeast Cells<br /> *Degradation of OsRAA1 Depends on the Proteasome<br /> *Two-Hybrid cDNA Library Construction and Screening<br /> *Protein Deletion Mutation and Yeast Plasmid Construction<br /> *Pull-Down Assay<br /> *Immunofluorescence of Rice Root Tips<br /> *Coimmunoprecipitation and Tris-Tricine Gel Electrophoresis<br /> *Colocalization of OsRAA1 and Tubulin in the BY-2 Cell Line<br /> *Transient Subcellular Localization<br /> *Determination of the Half-Life of OsRAA1<br /> *Protein Degradation Assay in Vitro<br /> *Morphological Examination of Transformed S. pombeCells<br /> <br /> ==References==<br /> *Rice ROOT ARCHITECTURE ASSOCIATED1 Binds the Proteasome Subunit RPT4 and Is Degraded in a D-Box and Proteasome-Dependent Manner Plant Physiology, 2008, 148(2): 843-855<br /> *Overexpression of OsRAA1 Causes Pleiotropic Phenotypes in Transgenic Rice Plants, including Altered Leaf, Flower, and Root Development and Root Response to Gravity Plant Physiology, 2004, 135(3): 1502-1513<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0257300|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001049166.1 GI:115435745 GeneID:4326229|<br /> Length = 785 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0257300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:8585137..8585921|<br /> CDS = 8585497..8585826|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MSGVWVFKNGVVRLVEKQQATAGTAVAGGRRKALVHTPSGQVVS SYAALEARLTALGWERYYEDPSLFQFHKRGSLDLISLPADFSAFSSVHMYDIVVKNRD SFRVVDA&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;96..425#tccatccacccatagctatctctagctagcttgcacattcttcattcttcttgcaatcagagctagagaaaaagagtttgagagagatctaagagatgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaaattgacctatgtataggctcgcatgcatgcaaggtagacgaccatccaccttgcatgcatgcaggctatcattctctcttcgtctccgtcttcgtctttcatctttgttgtggtgtgtgtgaatttattatacttcttatgactgtgtgtgtgactgtgtgagagttcatggagagtgatgagtagtggtatacatatgtgtcgtcgctgatcgatttggtttattaccttgtgggtgggtattaattatcagcagtgtgttatatcaattgcttacttgtatgtgtatcatgtacatgagtgagtgtggctgatgcatatatatagaggtctttaataattatgtactatatatttgtg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049166.1 RefSeq:Os01g0257300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0257300&diff=174836 2014-05-31T03:15:58Z

<p>Mayamei0424: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''OsRAA1'' (Oryza sativa Root Architecture Associated1) encodes a small protein (109 aa in length) conserved in plants including -GVWV/IF domain, -GWERYY domain ,-DLIS/ALP- and -H/YMYDI/VVV/I- domain. The OsRAA1 promoter(1,987 bp in length)includes elements of GA and auxin response, but it was a very weak response to GA3 treatment and it probably regulated by auxin. The OsRAA1 mRNA was specifically transcripted in the organs of roots and spikes, including root apex, cortex of root apical meristem ,pericycle of root apex, lateral roots, apical meristem of young spikes, collenchyma cells of margin vascular bundles between shoot and roots.In Ubi::OsRAA1 transgenic plants, overexpression ''OsRAA1'' in the root results in reduced growth of primary root, increased number of adventitious roots and helix primary root, and delayed gravitropic, which are similar to the phenotypes of the wild-type plant treated with auxin. Furthermore, the leaves and filaments are longer at the last stage of plant development in the transgenic plants. ''OsRAA1'' expression is induced by auxin. At the same time, overexpression of ''OsRAA1'' also causes endogenous indole-3-acetic acid to increase. These data suggest that ''OsRAA1'' as a new gene functions in the development of rice root systems,which are mediated by auxin&lt;ref name=&quot;ref1&quot;/&gt;.<br /> <br /> OsRAA1 Regulates Primary Root Development by Modulating Mitosis. OsRAA1 may function at the transition from metaphase to anaphase during cell division.OsRAA1 Interacts with OsRPT4 and Functions in Sister Chromatid Separation in Rice Root Cells.OsRAA1 was degraded by the 26S proteasome through interaction with OsRPT4,and APC perhaps serves to recognize OsRAA1 as E3.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> Immunoassay of a cell line with GFP alone showed immunofluorescence in the whole cell during the cell cycle.In contrast, in the OsRAA1-GFP transgenic line, OsRAA1 GFP was enriched at spindles, including tubulins, from metaphase to anaphase during cell division<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> *OsRAA1 Interaction Protein Screening<br /> *OsRAA1 Interacts with OsRPT4 in Vitro and in Vivo<br /> *Aberrant Mitosis Occurs in OsRAA1 Transgenic Rice and Transgenic Yeast Cells<br /> *Degradation of OsRAA1 Depends on the Proteasome<br /> *Two-Hybrid cDNA Library Construction and Screening<br /> *Protein Deletion Mutation and Yeast Plasmid Construction<br /> *Pull-Down Assay<br /> *Immunofluorescence of Rice Root Tips<br /> *Coimmunoprecipitation and Tris-Tricine Gel Electrophoresis<br /> *Colocalization of OsRAA1 and Tubulin in the BY-2 Cell Line<br /> *Transient Subcellular Localization<br /> *Determination of the Half-Life of OsRAA1<br /> *Protein Degradation Assay in Vitro<br /> *Morphological Examination of Transformed S. pombeCells<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;.Overexpression of OsRAA1 Causes Pleiotropic Phenotypes in Transgenic Rice Plants, including Altered Leaf, Flower, and Root Development and Root Response to Gravity Plant Physiology, 2004, 135(3): 1502-1513&lt;/ref&gt;<br /> *Rice ROOT ARCHITECTURE ASSOCIATED1 Binds the Proteasome Subunit RPT4 and Is Degraded in a D-Box and Proteasome-Dependent Manner Plant Physiology, 2008, 148(2): 843-855<br /> *Overexpression of OsRAA1 Causes Pleiotropic Phenotypes in Transgenic Rice Plants, including Altered Leaf, Flower, and Root Development and Root Response to Gravity Plant Physiology, 2004, 135(3): 1502-1513<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0257300|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001049166.1 GI:115435745 GeneID:4326229|<br /> Length = 785 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0257300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:8585137..8585921|<br /> CDS = 8585497..8585826|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MSGVWVFKNGVVRLVEKQQATAGTAVAGGRRKALVHTPSGQVVS SYAALEARLTALGWERYYEDPSLFQFHKRGSLDLISLPADFSAFSSVHMYDIVVKNRD SFRVVDA&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;96..425#tccatccacccatagctatctctagctagcttgcacattcttcattcttcttgcaatcagagctagagaaaaagagtttgagagagatctaagagatgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaaattgacctatgtataggctcgcatgcatgcaaggtagacgaccatccaccttgcatgcatgcaggctatcattctctcttcgtctccgtcttcgtctttcatctttgttgtggtgtgtgtgaatttattatacttcttatgactgtgtgtgtgactgtgtgagagttcatggagagtgatgagtagtggtatacatatgtgtcgtcgctgatcgatttggtttattaccttgtgggtgggtattaattatcagcagtgtgttatatcaattgcttacttgtatgtgtatcatgtacatgagtgagtgtggctgatgcatatatatagaggtctttaataattatgtactatatatttgtg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049166.1 RefSeq:Os01g0257300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0257300&diff=174833 2014-05-31T03:13:26Z

<p>Mayamei0424: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> ''OsRAA1'' (Oryza sativa Root Architecture Associated1) encodes a small protein (109 aa in length) conserved in plants including -GVWV/IF domain, -GWERYY domain ,-DLIS/ALP- and -H/YMYDI/VVV/I- domain. The OsRAA1 promoter(1,987 bp in length)includes elements of GA and auxin response, but it was a very weak response to GA3 treatment and it probably regulated by auxin. The OsRAA1 mRNA was specifically transcripted in the organs of roots and spikes, including root apex, cortex of root apical meristem ,pericycle of root apex, lateral roots, apical meristem of young spikes, collenchyma cells of margin vascular bundles between shoot and roots.In Ubi::OsRAA1 transgenic plants, overexpression ''OsRAA1'' in the root results in reduced growth of primary root, increased number of adventitious roots and helix primary root, and delayed gravitropic, which are similar to the phenotypes of the wild-type plant treated with auxin. Furthermore, the leaves and filaments are longer at the last stage of plant development in the transgenic plants. ''OsRAA1'' expression is induced by auxin. At the same time, overexpression of ''OsRAA1'' also causes endogenous indole-3-acetic acid to increase. These data suggest that ''OsRAA1'' as a new gene functions in the development of rice root systems,which are mediated by auxin&lt;ref name=&quot;ref1&quot;/&gt;.<br /> <br /> OsRAA1 Regulates Primary Root Development by Modulating Mitosis. OsRAA1 may function at the transition from metaphase to anaphase during cell division.OsRAA1 Interacts with OsRPT4 and Functions in Sister Chromatid Separation in Rice Root Cells.OsRAA1 was degraded by the 26S proteasome through interaction with OsRPT4,and APC perhaps serves to recognize OsRAA1 as E3.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> Immunoassay of a cell line with GFP alone showed immunofluorescence in the whole cell during the cell cycle.In contrast, in the OsRAA1-GFP transgenic line, OsRAA1 GFP was enriched at spindles, including tubulins, from metaphase to anaphase during cell division<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> *OsRAA1 Interaction Protein Screening<br /> *OsRAA1 Interacts with OsRPT4 in Vitro and in Vivo<br /> *Aberrant Mitosis Occurs in OsRAA1 Transgenic Rice and Transgenic Yeast Cells<br /> *Degradation of OsRAA1 Depends on the Proteasome<br /> *Two-Hybrid cDNA Library Construction and Screening<br /> *Protein Deletion Mutation and Yeast Plasmid Construction<br /> *Pull-Down Assay<br /> *Immunofluorescence of Rice Root Tips<br /> *Coimmunoprecipitation and Tris-Tricine Gel Electrophoresis<br /> *Colocalization of OsRAA1 and Tubulin in the BY-2 Cell Line<br /> *Transient Subcellular Localization<br /> *Determination of the Half-Life of OsRAA1<br /> *Protein Degradation Assay in Vitro<br /> *Morphological Examination of Transformed S. pombeCells<br /> <br /> ==References==<br /> Please input cited references here.<br /> *Rice ROOT ARCHITECTURE ASSOCIATED1 Binds the Proteasome Subunit RPT4 and Is Degraded in a D-Box and Proteasome-Dependent Manner Plant Physiology, 2008, 148(2): 843-855<br /> *Overexpression of OsRAA1 Causes Pleiotropic Phenotypes in Transgenic Rice Plants, including Altered Leaf, Flower, and Root Development and Root Response to Gravity Plant Physiology, 2004, 135(3): 1502-1513<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0257300|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001049166.1 GI:115435745 GeneID:4326229|<br /> Length = 785 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0257300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:8585137..8585921|<br /> CDS = 8585497..8585826|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MSGVWVFKNGVVRLVEKQQATAGTAVAGGRRKALVHTPSGQVVS SYAALEARLTALGWERYYEDPSLFQFHKRGSLDLISLPADFSAFSSVHMYDIVVKNRD SFRVVDA&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;96..425#tccatccacccatagctatctctagctagcttgcacattcttcattcttcttgcaatcagagctagagaaaaagagtttgagagagatctaagagatgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaaattgacctatgtataggctcgcatgcatgcaaggtagacgaccatccaccttgcatgcatgcaggctatcattctctcttcgtctccgtcttcgtctttcatctttgttgtggtgtgtgtgaatttattatacttcttatgactgtgtgtgtgactgtgtgagagttcatggagagtgatgagtagtggtatacatatgtgtcgtcgctgatcgatttggtttattaccttgtgggtgggtattaattatcagcagtgtgttatatcaattgcttacttgtatgtgtatcatgtacatgagtgagtgtggctgatgcatatatatagaggtctttaataattatgtactatatatttgtg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049166.1 RefSeq:Os01g0257300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Mayamei0424 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0257300&diff=174657 2014-05-30T14:42:48Z

<p>Mayamei0424: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> OsRAA1 encodes a 12.0-kD protein that has 58% homology to the AtFPF1 (Flowering Promoting Factor 1) in Arabidopsis, which has not been reported as modulating<br /> root development yet.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0257300|<br /> Description = Conserved hypothetical protein|<br /> Version = NM_001049166.1 GI:115435745 GeneID:4326229|<br /> Length = 785 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0257300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:8585137..8585921|<br /> CDS = 8585497..8585826|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:8585137..8585921<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaa&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MSGVWVFKNGVVRLVEKQQATAGTAVAGGRRKALVHTPSGQVVS SYAALEARLTALGWERYYEDPSLFQFHKRGSLDLISLPADFSAFSSVHMYDIVVKNRD SFRVVDA&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;96..425#tccatccacccatagctatctctagctagcttgcacattcttcattcttcttgcaatcagagctagagaaaaagagtttgagagagatctaagagatgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaaattgacctatgtataggctcgcatgcatgcaaggtagacgaccatccaccttgcatgcatgcaggctatcattctctcttcgtctccgtcttcgtctttcatctttgttgtggtgtgtgtgaatttattatacttcttatgactgtgtgtgtgactgtgtgagagttcatggagagtgatgagtagtggtatacatatgtgtcgtcgctgatcgatttggtttattaccttgtgggtgggtattaattatcagcagtgtgttatatcaattgcttacttgtatgtgtatcatgtacatgagtgagtgtggctgatgcatatatatagaggtctttaataattatgtactatatatttgtg&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049166.1 RefSeq:Os01g0257300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Mayamei0424