http://192.168.164.12:81/ricewiki/api.php?action=feedcontributions&feedformat=atom&user=Mmlin66 2026-08-27T17:26:07Z User contributions MediaWiki 1.30.0 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=171032 2014-05-23T03:03:06Z

<p>Mmlin66: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> <br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;ref1&quot; /&gt; It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.&lt;ref name=&quot;ref1&quot; /&gt;&lt;ref name=&quot;ref2&quot; /&gt; The two domain regions are spaced by a long linker sequence (amino acids 116-553).&lt;ref name=&quot;ref1&quot; /&gt; This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A)&lt;ref name=&quot;ref1&quot; /&gt;. These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation.&lt;ref name=&quot;ref1&quot; /&gt;&lt;ref name=&quot;ref3&quot; /&gt; The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6)&lt;ref name=&quot;ref1&quot; /&gt; '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8)&lt;ref name=&quot;ref1&quot; /&gt; In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)&lt;ref name=&quot;ref1&quot; /&gt;<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene.&lt;ref name=&quot;ref1&quot; /&gt; The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages.&lt;ref name=&quot;ref1&quot; /&gt; '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4).&lt;ref name=&quot;ref1&quot; /&gt; OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45.&lt;ref name=&quot;ref1&quot; /&gt; The protein is a nucleus-localizing protein.(Figure 2B)&lt;ref name=&quot;ref1&quot; /&gt;[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots.&lt;ref name=&quot;ref1&quot; /&gt; Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs.(Figure 3C)&lt;ref name=&quot;ref1&quot; /&gt; This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)&lt;ref name=&quot;ref4&quot; /&gt;<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C))&lt;ref name=&quot;ref1&quot; /&gt; In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation.&lt;ref name=&quot;ref1&quot; /&gt; It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion.&lt;ref name=&quot;ref1&quot; /&gt; In addition, '''''OsRad21-4''''' is required for chromosome condensation.&lt;ref name=&quot;ref1&quot; /&gt; Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes.&lt;ref name=&quot;ref1&quot; /&gt; Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I&lt;ref name=&quot;ref5&quot; /&gt;, suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> * &lt;ref name=&quot;ref1&quot;&gt;<br /> Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/ref&gt;<br /> * &lt;ref name=&quot;ref2&quot;&gt;<br /> Zhang L R, Tao J Y, Wang T. Molecular characterization of OsRAD21‐1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion*[J]. Journal of experimental botany, 2004, 55(399): 1149-1152.&lt;/ref&gt;<br /> *&lt;ref name=&quot;ref3&quot;&gt;Nasmyth K. Disseminating the genome: joining, resolving, and separating sister chromatids during mitosis and meiosis[J]. Annual review of genetics, 2001, 35(1): 673-745.&lt;/ref&gt;<br /> *&lt;ref name=&quot;ref4&quot;&gt;Watanabe Y, Yokobayashi S, Yamamoto M, et al. Pre-meiotic S phase is linked to reductional chromosome segregation and recombination[J]. Nature, 2001, 409(6818): 359-363.&lt;/ref&gt;<br /> *&lt;ref name=&quot;ref5&quot;&gt;Ma H. Molecular genetic analyses of microsporogenesis and microgametogenesis in flowering plants[J]. Annu. Rev. Plant Biol., 2005, 56: 393-434.&lt;/ref&gt;<br /> &lt;references&gt;<br /> <br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=171028 2014-05-23T02:57:01Z

<p>Mmlin66: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> <br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;ref1&quot; /&gt;. It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.&lt;ref name=&quot;ref1&quot; /&gt;&lt;ref name=&quot;ref2&quot; /&gt; The two domain regions are spaced by a long linker sequence (amino acids 116-553).&lt;ref name=&quot;ref1&quot; /&gt; This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A).&lt;ref name=&quot;ref1&quot; /&gt; These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation.&lt;ref name=&quot;ref1&quot; /&gt;&lt;ref name=&quot;ref3&quot; /&gt; The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6)&lt;ref name=&quot;ref1&quot; /&gt; '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)&lt;ref name=&quot;ref1&quot; /&gt;<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene.&lt;ref name=&quot;ref1&quot; /&gt; The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages.&lt;ref name=&quot;ref1&quot; /&gt; '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4).&lt;ref name=&quot;ref1&quot; /&gt; OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45.&lt;ref name=&quot;ref1&quot; /&gt; The protein is a nucleus-localizing protein.&lt;ref name=&quot;ref1&quot; /&gt;(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots.&lt;ref name=&quot;ref1&quot; /&gt; Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs.&lt;ref name=&quot;ref1&quot; /&gt; (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)&lt;ref name=&quot;ref4&quot; /&gt;<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C))&lt;ref name=&quot;ref1&quot; /&gt; In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation.&lt;ref name=&quot;ref1&quot; /&gt; It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion.&lt;ref name=&quot;ref1&quot; /&gt; In addition, '''''OsRad21-4''''' is required for chromosome condensation.&lt;ref name=&quot;ref1&quot; /&gt; Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes.&lt;ref name=&quot;ref1&quot; /&gt; Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I&lt;ref name=&quot;ref5&quot; /&gt;, suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> * &lt;ref name=&quot;ref1&quot;&gt;<br /> Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/ref&gt;<br /> * &lt;ref name=&quot;ref2&quot;&gt;<br /> Zhang L R, Tao J Y, Wang T. Molecular characterization of OsRAD21‐1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion*[J]. Journal of experimental botany, 2004, 55(399): 1149-1152.&lt;/ref&gt;<br /> *&lt;ref name=&quot;ref3&quot;&gt;Nasmyth K. Disseminating the genome: joining, resolving, and separating sister chromatids during mitosis and meiosis[J]. Annual review of genetics, 2001, 35(1): 673-745.&lt;/ref&gt;<br /> *&lt;ref name=&quot;ref4&quot;&gt;Watanabe Y, Yokobayashi S, Yamamoto M, et al. Pre-meiotic S phase is linked to reductional chromosome segregation and recombination[J]. Nature, 2001, 409(6818): 359-363.&lt;/ref&gt;<br /> *&lt;ref name=&quot;ref5&quot;&gt;Ma H. Molecular genetic analyses of microsporogenesis and microgametogenesis in flowering plants[J]. Annu. Rev. Plant Biol., 2005, 56: 393-434.&lt;/ref&gt;<br /> &lt;references&gt;<br /> <br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=171022 2014-05-23T02:52:38Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> <br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;ref1&quot; /&gt;. It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.&lt;ref name=&quot;ref1&quot; /&gt;[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> * &lt;ref name=&quot;ref1&quot;&gt;<br /> Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/ref&gt;<br /> * &lt;ref name=&quot;ref2&quot;&gt;<br /> Zhang L R, Tao J Y, Wang T. Molecular characterization of OsRAD21‐1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion*[J]. Journal of experimental botany, 2004, 55(399): 1149-1152.&lt;/ref&gt;<br /> *&lt;ref name=&quot;ref3&quot;&gt;Nasmyth K. Disseminating the genome: joining, resolving, and separating sister chromatids during mitosis and meiosis[J]. Annual review of genetics, 2001, 35(1): 673-745.&lt;/ref&gt;<br /> *&lt;ref name=&quot;ref4&quot;&gt;Watanabe Y, Yokobayashi S, Yamamoto M, et al. Pre-meiotic S phase is linked to reductional chromosome segregation and recombination[J]. Nature, 2001, 409(6818): 359-363.&lt;/ref&gt;<br /> *&lt;ref name=&quot;ref5&quot;&gt;Ma H. Molecular genetic analyses of microsporogenesis and microgametogenesis in flowering plants[J]. Annu. Rev. Plant Biol., 2005, 56: 393-434.&lt;/ref&gt;<br /> &lt;references&gt;<br /> <br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=171015 2014-05-23T02:45:58Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> <br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;ref1&quot; /&gt;. It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.&lt;ref name=&quot;ref1&quot; /&gt;[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> * &lt;ref name=&quot;ref1&quot;&gt;<br /> Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.<br /> &lt;/ref&gt;<br /> <br /> &lt;references&gt;<br /> * &lt;ref name=&quot;ref2&quot;&gt;<br /> Zhang L R, Tao J Y, Wang T. Molecular characterization of OsRAD21‐1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion*[J]. Journal of experimental botany, 2004, 55(399): 1149-1152.<br /> &lt;/ref&gt;<br /> <br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=171014 2014-05-23T02:45:38Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> <br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;ref1&quot; /&gt;. It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.&lt;ref name=&quot;ref1&quot; /&gt;[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> * &lt;ref name=&quot;ref1&quot;&gt;<br /> Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.<br /> &lt;/ref&gt;<br /> <br /> &lt;references&gt;<br /> * &lt;ref name=&quot;ref&quot;&gt;<br /> Zhang L R, Tao J Y, Wang T. Molecular characterization of OsRAD21‐1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion*[J]. Journal of experimental botany, 2004, 55(399): 1149-1152.<br /> &lt;/ref&gt;<br /> <br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=171011 2014-05-23T02:44:19Z

<p>Mmlin66: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> <br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;ref1&quot; /&gt;. It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.&lt;ref name=&quot;ref1&quot; /&gt;[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> * &lt;ref name=&quot;ref1&quot;&gt;<br /> Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.<br /> &lt;/ref&gt;<br /> <br /> <br /> <br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=171010 2014-05-23T02:43:35Z

<p>Mmlin66: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> <br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;ref1&quot; /&gt;.&lt;br&gt;&lt;br&gt; It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> * &lt;ref name=&quot;ref1&quot;&gt;<br /> Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.<br /> &lt;/ref&gt;<br /> <br /> <br /> <br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=171009 2014-05-23T02:42:45Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.[[&lt;ref name=&quot;[1]&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; ]]<br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> * &lt;ref name=&quot;ref1&quot;&gt;<br /> Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.<br /> &lt;/ref&gt;<br /> <br /> <br /> <br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=171004 2014-05-23T02:32:27Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.[[&lt;ref name=&quot;[1]&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; ]]<br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.[[&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;[[1]]]]<br /> <br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=171003 2014-05-23T02:31:58Z

<p>Mmlin66: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.[[&lt;ref name=&quot;[1]&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; ]]<br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;[[1]]<br /> <br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=171001 2014-05-23T02:29:23Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.&lt;ref name=&quot;[1]&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; [[&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;]]<br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;[[1]]<br /> <br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=171000 2014-05-23T02:28:59Z

<p>Mmlin66: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.&lt;ref name=&quot;[1]&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; [[&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;]]<br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170998 2014-05-23T02:27:39Z

<p>Mmlin66: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.&lt;ref name=&quot;[1]&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; <br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170997 2014-05-23T02:27:06Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; <br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170996 2014-05-23T02:26:43Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; <br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.<br /> &lt;references /&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&gt;<br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170995 2014-05-23T02:26:23Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; <br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> &lt;references /&gt;<br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170993 2014-05-23T02:23:46Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; <br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> <br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170992 2014-05-23T02:23:11Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; <br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;ref name=&quot;&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&quot; /&gt;<br /> <br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170991 2014-05-23T02:22:41Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; <br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;ref name=&quot;1&quot; /&gt;<br /> <br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170990 2014-05-23T02:22:20Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; <br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> <br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> &lt;ref name=&quot;foo&quot; /&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170988 2014-05-23T02:21:51Z

<p>Mmlin66: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; <br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref name=&quot;1&quot;&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> <br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170984 2014-05-23T02:20:30Z

<p>Mmlin66: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.&lt;ref&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; <br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> <br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170982 2014-05-23T02:18:39Z

<p>Mmlin66: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.&lt;ref&gt;1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;&lt;/ref&gt; It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> <br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170979 2014-05-23T02:06:56Z

<p>Mmlin66: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.[1] It encodes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, '''''OsRad21-4''''' is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> <br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170884 2014-05-22T15:36:40Z

<p>Mmlin66: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''''OsRAD21-4''''', a '''''RAD21'''''-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> '''''OsRad21-4''''' is composed of 20 exons and 19 introns.[1] It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the '''''Rad21/Rec8''''' family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of '''''OsRad21-4''''' at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) '''''OsRad21-4''''' was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> '''''OsRad21-4''''' is present in the rice genome as a single-copy gene. The mRNA and protein of '''''OsRad21-4''''' were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. '''''OsRAD21-4''''' cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, '''''OsRad21-4''''' was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, '''''OsRad21-4''''' was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> '''''OsRad21-4''''' is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, '''''OsRad21-4''''' is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, '''''OsRad21-4''''' is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> <br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170881 2014-05-22T15:31:21Z

<p>Mmlin66: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. '''OsRAD21-4'''''斜体文字'', a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns.[1] It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> <br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170879 2014-05-22T15:30:49Z

<p>Mmlin66: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> '''''Rad21/Rec8''''' is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns.[1] It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> <br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170877 2014-05-22T15:28:23Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns.[1] It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> <br /> 2.&lt;nowiki&gt;Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion&lt;/nowiki&gt;<br /> <br /> 3.&lt;nowiki&gt;DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis&lt;/nowiki&gt;<br /> <br /> 4.&lt;nowiki&gt;Pre-meiotic S phase is linked to reductional chromosome segregation and recombination&lt;/nowiki&gt;<br /> <br /> 5.&lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170875 2014-05-22T15:16:08Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns.[1] It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.[[1]]&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> <br /> <br /> 2.[[File:Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion.pdf]]<br /> <br /> 3.[[File:DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis.pdf]]<br /> <br /> 4.[[File:Pre-meiotic S phase is linked to reductional chromosome segregation and recombination .pdf]]<br /> <br /> 5.[[File:Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant.pdf]]<br /> <br /> &lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170873 2014-05-22T15:15:51Z

<p>Mmlin66: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns.[1] It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> <br /> <br /> 2.[[File:Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion.pdf]]<br /> <br /> 3.[[File:DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis.pdf]]<br /> <br /> 4.[[File:Pre-meiotic S phase is linked to reductional chromosome segregation and recombination .pdf]]<br /> <br /> 5.[[File:Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant.pdf]]<br /> <br /> &lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170871 2014-05-22T15:15:06Z

<p>Mmlin66: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns.[[&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;]] It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> <br /> <br /> 2.[[File:Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion.pdf]]<br /> <br /> 3.[[File:DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis.pdf]]<br /> <br /> 4.[[File:Pre-meiotic S phase is linked to reductional chromosome segregation and recombination .pdf]]<br /> <br /> 5.[[File:Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant.pdf]]<br /> <br /> &lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170870 2014-05-22T15:14:38Z

<p>Mmlin66: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt; It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> <br /> <br /> 2.[[File:Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion.pdf]]<br /> <br /> 3.[[File:DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis.pdf]]<br /> <br /> 4.[[File:Pre-meiotic S phase is linked to reductional chromosome segregation and recombination .pdf]]<br /> <br /> 5.[[File:Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant.pdf]]<br /> <br /> &lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170869 2014-05-22T15:13:46Z

<p>Mmlin66: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns.[[Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.]] It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> <br /> <br /> 2.[[File:Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion.pdf]]<br /> <br /> 3.[[File:DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis.pdf]]<br /> <br /> 4.[[File:Pre-meiotic S phase is linked to reductional chromosome segregation and recombination .pdf]]<br /> <br /> 5.[[File:Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant.pdf]]<br /> <br /> &lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170868 2014-05-22T15:12:40Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns. It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.&lt;nowiki&gt;Zhang L, Tao J, Wang S, et al. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis[J]. Plant molecular biology, 2006, 60(4): 533-554.&lt;/nowiki&gt;<br /> <br /> <br /> 2.[[File:Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion.pdf]]<br /> <br /> 3.[[File:DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis.pdf]]<br /> <br /> 4.[[File:Pre-meiotic S phase is linked to reductional chromosome segregation and recombination .pdf]]<br /> <br /> 5.[[File:Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant.pdf]]<br /> <br /> &lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170867 2014-05-22T15:06:53Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns. It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.[[File:The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis.pdf]]<br /> <br /> 2.[[File:Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion.pdf]]<br /> <br /> 3.[[File:DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis.pdf]]<br /> <br /> 4.[[File:Pre-meiotic S phase is linked to reductional chromosome segregation and recombination .pdf]]<br /> <br /> 5.[[File:Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant.pdf]]<br /> <br /> &lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170866 2014-05-22T15:06:35Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns. It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.[[File:The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis.pdf]]<br /> <br /> 2.[[File:Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion.pdf]]<br /> <br /> 3.[[File:DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis.pdf]]<br /> <br /> 4.[[File:Pre-meiotic S phase is linked to reductional chromosome segregation and recombination .pdf]]<br /> <br /> 5.[[File:Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant.pdf]]<br /> <br /> &lt;nowiki&gt;Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plant.pdf&lt;/nowiki&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170865 2014-05-22T15:05:31Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns. It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.[[File:The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis.pdf]]<br /> <br /> 2.[[File:Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion.pdf]]<br /> <br /> 3.[[File:DISSEMINATING THE GENOME: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis.pdf]]<br /> <br /> 4.[[File:Pre-meiotic S phase is linked to reductional chromosome segregation and recombination .pdf]]<br /> <br /> 5.[[File:Molecular Genetic Analyses of Microsporogenesis and Microgametogenesis in Flowering Plan.pdf]]<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:1.pdf&diff=170862 2014-05-22T14:59:22Z

<p>Mmlin66: </p> <hr /> <div></div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170861 2014-05-22T14:59:01Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns. It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> 1.[[File:1.pdf]]<br /> 2.[[File:2.pdf]]<br /> 3.[[File:3.pdf]]<br /> 4.[[File:4.pdf]]<br /> 5.[[File:5.pdf]]<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170860 2014-05-22T14:58:25Z

<p>Mmlin66: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns. It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> [[File:1.pdf]]<br /> [[File:2.pdf]]<br /> [[File:3.pdf]]<br /> [[File:4.pdf]]<br /> [[File:5.pdf]]<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170848 2014-05-22T14:42:26Z

<p>Mmlin66: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns. It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> 6.Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan<br /> <br /> 7.Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, 3-11-1 Asahigaoka, Osaka 582-0026, Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170839 2014-05-22T14:32:11Z

<p>Mmlin66: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns. It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> <br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> <br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> <br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170833 2014-05-22T14:22:01Z

<p>Mmlin66: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns. It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> 3.Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan<br /> 4.PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan<br /> 5.Imperial Cancer Research Fund, 44 Lincoln's Inn Field, London WC2A 3PX, UK<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170832 2014-05-22T14:16:32Z

<p>Mmlin66: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns. It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular &amp; Developmental Biology, Institute of Botany, Chinese academy of Sciences, Beijing 100093,China<br /> 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170831 2014-05-22T14:11:16Z

<p>Mmlin66: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns. It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> Key Laboratory of Photosynthesis and Environmental Molecular Physiology<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170830 2014-05-22T14:10:33Z

<p>Mmlin66: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns. It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I[5], suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170828 2014-05-22T14:05:13Z

<p>Mmlin66: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns. It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I (Ma, 2005), suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:F2C.png&diff=170826 2014-05-22T13:57:12Z

<p>Mmlin66: (C) Phylogenetic analysis revealed that OsRad21-4 was grouped with Rec8-like proteins from other species and formed a clade with Arabidopsis Syn1. The tree was derived from the multiple alignments of N-terminal regions of OsRad21-4 and other Rad21/Rec8 se</p> <hr /> <div>(C) Phylogenetic analysis revealed that OsRad21-4 was grouped with Rec8-like proteins from other species and formed a<br /> clade with Arabidopsis Syn1. The tree was derived from the multiple alignments of N-terminal regions of OsRad21-4 and other<br /> Rad21/Rec8 sequences with use of Clustal W plus a neighbor-joining feature with removal of most distance sequences. These sequences<br /> are shown as follows: OsRad21–1 and OsRad21-4 (this study, Oryza sativa, accession No. AY28893 and AY371049);<br /> Syn1, Syn2 and Syn3 (Arabidopsis thaliana, accession No. AF080619, AF281154 and AF281155); HsRad21 and HsRec8 (Homo<br /> sapiens, accession No. NM_006265 and NM_005132); MmRad21 and MmRec8 (Mus muculus, accession No. NP_033035 and<br /> AAF69524); CeRec8 (Caenorhabditis elegans, accession No. CAB05309); DmRad21 (Drosophila melanogaster, accession No.<br /> NM_143900); XRad21 (Xenopus laevis, accession No. AF051786); and SpRec8 (Schizosaccharomyces pombe, accession No.<br /> AB018077). Bootstrap values, evaluated for 100 bootstrap trails, are shown at branch points</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170825 2014-05-22T13:55:27Z

<p>Mmlin66: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns. It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. <br /> disrupt homologous pairing and at sister arm cohesion, but not centromere cohesion<br /> <br /> In summary, OsRad21-4-deficient plants mediated by RNAi showed multiple aberrant events at meiosis prophase I, including over-condensation of chromosomes, precocious segregation of homologues and chromosome fragmentation.<br /> <br /> Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I (Ma, 2005), such as PAIR1 in rice for pairing (Nonomura et al., 2004) and PHS1 in maize for pairing, recombination and synapsis (Pawlowski et al., 2004), suggesting possible interaction of early events of prophase I.<br /> [[File:F2C.png|200px|thumb|left|Figure2(C)]]<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0580500&diff=170824 2014-05-22T13:54:39Z

<p>Mmlin66: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> Rad21/Rec8 is an important component and key regulator of cohesins. OsRAD21-4, a RAD21-like gene from rice Zhonghua 10 (Oryza sativa L. ssp.japonica), is a single-copy gene in the rice genome and essential for efficient meiosis.<br /> ===Function===<br /> OsRad21-4 is composed of 20 exons and 19 introns. It codes a relatively hydrophilic protein and contains the entire Pfam04825 and Pfam04824 domains (spanning amino acids 1–115 and 554–608, respectively) (Figure 2A), of which Pfam04825 is highly conserved in all known members and Pfam04824 present in most of the Rad21/Rec8 family.[1,2] The two domain regions are spaced by a long linker sequence (amino acids 116-553). This linker sequence contains a potential nuclear targeting motif at positions 272–279 (KRKKRRKD), 2 separase recognition sites at 411–421 (ADDIEKLRGNT) and 420–430 (NTSGEYGRDYD) and a PEST motif at 511– 534 (RLSDVGPTPDLLEEIEPTQTPYEK) (Figure 2A). These motifs are proposed to be implicated in function regulation in cohesion establishment and disassociation [1,3]. The deficiency of OsRad21-4 at the mRNA and protein is correlated with pollen cell sterility phenotype.(Figure6) OsRad21-4 was essential to meiosis. (Figure7, Figure8) In addtion, OsRad21-4 is responsible for the pairing of some homologous chromosomes.(Figure9, Figure10)<br /> [[File:F22.png|200px|thumb|left|Figure2(A)]][[File:F6.png|200px|thumb|left|Figure6]][[File:F7.png|200px|thumb|left|Figure7]][[File:F8.png|200px|thumb|left|Figure8]][[File:F9.png|200px|thumb|left|Figure9]][[File:F10.png|200px|thumb|left|Figure10]]<br /> <br /> ===Expression===<br /> OsRad21-4 is present in the rice genome as a single-copy gene. The mRNA and protein of OsRad21-4 were expressed preferentially in young flowers where the pollen mother cells (PMCs) were in pre-meiotic stages. OsRAD21-4 cDNA cloned here is 2133 bp long and has a 5' UTR of 54 bp, a 3' UTR of 255 bp and an ORF of 1824 bp encoding a deduced polypeptide of 608 amino acids (OsRad21-4). OsRad21-4 has a calculated molecular mass of 68.5 kDa and an isoelectric point (pI) of 5.45. The protein is a nucleus-localizing protein.(Figure 2B)[[File:F23.png|200px|thumb|left|Figure2(B)]] As shown in Figure 3A, OsRad21-4 was expressed preferentially in flowers, weakly in leaves and barely in buds and roots. Furthermore, OsRad214 was expressed dominantly just before the premeiotic stage of PMCs. (Figure 3C) This gene should function in premeiotic and meiotic PMCs, which is consistent with this notion that meiotic cohesion is established at the pre-meiotic S phase. (Figur 4)[(Watanabe et al.,2001)4]<br /> [[File:F3A.png|200px|thumb|left|Figure3 (A)]][[File:F3C.png|200px|thumb|left|Figure3(C)]][[File:F4.png|200px|thumb|left|Figure4]]<br /> <br /> ===Evolution===<br /> OsRad21-4 is a rice orthologue of yeast Rec8.(Figure2(C)) In rice, OsRad21-4 is required for homologous pairing and segregation. It might be responsible mainly for sister chromatid-arm cohesion and to a lesser extent or not at all for centromere cohesion. In addition, OsRad21-4 is required for chromosome condensation. Besides, Zhang et al suggest possible link between Rec8 proteins and chromosome fragmentation in higher eukaryotes. <br /> disrupt homologous pairing and at sister arm cohesion, but not centromere cohesion<br /> <br /> In summary, OsRad21-4-deficient plants mediated by RNAi showed multiple aberrant events at meiosis prophase I, including over-condensation of chromosomes, precocious segregation of homologues and chromosome fragmentation.<br /> <br /> Furthermore, appearance of micronuclei and/or undetached dinuclei-containing spores and unequal cell division at anaphase I and II in the deficient plants were similar to those reported in mutants of genes related to other early events of meiosis prophase I (Ma, 2005), such as PAIR1 in rice for pairing (Nonomura et al., 2004) and PHS1 in maize for pairing, recombination and synapsis (Pawlowski et al., 2004), suggesting possible interaction of early events of prophase I.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0580500|<br /> Description = Rad21/Rec8 like protein, N-terminal domain containing protein|<br /> Version = NM_001062961.1 GI:115465652 GeneID:4339720|<br /> Length = 7262 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0580500, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:28971202..28978463|<br /> CDS = 28971255..28971307,28971498..28971566,28971665..28971723,28971819..28971863,28971950..28971969&lt;br&gt;,28972078..28972154,28973352..28973448,28973606..28973687,28973767..28973845&lt;br&gt;,28973955..28974118,28974803..28974846,28974927..28975082,28975179..28975347&lt;br&gt;,28975463..28975530,28975642..28975789,28975853..28975940,28976938..28977065&lt;br&gt;,28977165..28977249,28977333..28977445,28978161..28978243|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:28971202..28978463<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctgtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatgatgatgtgtctcggtttctgattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatgcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctgggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcgttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MFYSHQLLARKAPLGQIWMAATLHSKINRKRLDKLDIIKICEEI LNPSVPMALRLSGILMGGVAIVYERKVKALYDDVSRFLIEINEAWRVKPVADPTVLPK GKTQAKYEAVTLPENIMDMDVEQPMLFSEADTTRFRGMRLEDLDDQYINVNLDDDDFS RAENHHQADAENITLADNFGSGLGETDVFNRFERFDITDDDATFNVTPDGHPQVPSNL VPSPPRQEDSPQQQENHHAASSPLHEEAQQGGASVKNEQEQQKMKGQQPAKSSKRKKR RKDDEVMMDNDQIMIPGNVYQTWLKDPSSLITKRHRINSKVNLIRSIKIRDLMDLPLV SLISSLEKSPLEFYYPKELMQLWKECTEVKSPKAPSSGGQQSSSPEQQQRNLPPQAFP TQPQVDNDREMGFHPVDFADDIEKLRGNTSGEYGRDYDAFHSDHSVTPGSPGLSRRSA SSSGGSGRGFTQLDPEVQLPSGRSKRQHSSGKSFGNLDPVEEEFPFEQELRDFKMRRL SDVGPTPDLLEEIEPTQTPYEKKSNPIDQVTQSIHSYLKLHFDTPGASQSESLSQLAH GMTTAKAARLFYQACVLATHDFIKVNQLEPYGDILISRGPKM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;54..106#297..365#464..522#618..662#749..768#877..953#2151..2247#2405..2486#2566..2644#2754..2917#3602..3645#3726..3881#3978..4146#4262..4329#4441..4588#4652..4739#5737..5864#5964..6048#6132..6244#6960..7042#cagcgcctccactctcactcgctcatccattccctccctcctcacgatcgagaatgttctactcgcaccagctcctcgcgcggaaggctccgctcggccagatatggtgggtttgcttcgctctccgctcctctcgatcgcgtcctttcggcttctcttctcatgcgccgttctgcggtgctccgttctatattgttttctgcgatttctctgctcgttccgctccgaaatccgaatgttcgccacttggttggagttgattaggccgctgattactccgcttttttttttcgcaggatggcggcgacgcttcactcgaagatcaaccggaagcggcttgacaagctcgacatcatcaaaatctggtgagcgaattagtccgaatccagttgtctctgatttcttcgtttcgaacttgacggttttttttggggggattttgtgcgtgtggtttttgggttagtgaggagattttgaacccgtcggtacccatggcactaaggctctccggaattctcatgggtgagtccagtttgcttgttgtctccatagttccgatcgcatttgtttggtgatattttctgatgtgggtgcttgcgtcgtgggagtgtgagtaggtggtgtggcgatcgtgtacgagaggaaggtgaaggctctgtatggtgagttgctccctgattgcttctcttttgtttccattttttttggattcgttatagactcaaactgtgtgagatcttcttcgcagatgatgtgtctcggtttctggtaaaattcgagttcgattttcacctaattttggtgcctcctccttgatccatttgcctgtctatgctaacatggttcagattacgcttgtactaactcctcacacagattgagatcaacgaggcatggcgggtcaagccagtcgcagaccccaccgtacttcccaagggcaaaacccaagccaagtaagtgccctctttgtttccatgaccttctaggactagaagtttctagatgttctgtgcacggttttgggttcgagggaagattgaagaacatgctattatgttggtgaccgaggggagtttgcttttgtttccctgctgccagggattgtgagtttgtgtgtgattttcaccatccgatgctaatctgatggactcgtcatttgacacagttgcaagttttgatatgtcatcagcttacttggttctgattagaacatgctgcatctgtgtaatgttgattttatgatgatactatctccttgcagcatagtacattgttatgttttcagatgtacctgatgttgtcatatgactgattgttctgttctacctgttgtctattggcggttcatgggaccctgctgttacttgcctcttgttgtcttgctgactttggtagcagccgatgtctgtattatgcaggagtactattctacaaatgatctgtgtttcctggcagcataaatttgtctgtatgttgttccagtatatgtaggtcctctacatttgtagggtgatgaagggcttttgcttccccacagtgtttcctcctctcttaatgaaatgatatacaactctcttgcatattcaagaaaaaaaaactggaagggcattaggcagttaggcaggcattgtcagtgagcgatacatgctttatggggatgaacatttgactttttcttgggtgtcagttactggtgccaagggagcaggtctgagggccgtctggtacttgagaaatgttgctatcttagagtctagggaattccatcacctttgggaaccggaggcaaacatttcttaatcttgtatcatgctcgaaggactgactcttgtgggtgtaccatttccttccattgtggcctgcatctgatatgtaaccatgtggctgtgccatcatccccttgatcccttcggaactatgttcgatagttactgcaatgttcacgtgatgttgatacctgtactgttgcaatgagtttaaccctcttggagtcctggatagttcctctgaatgattgtatcccaagggtcttttcaacttacttttgtaaatagcatagagtgggtataagttggtggatggagaatgatattttgtaccaagagtaaaacagtaatggtttgctcatctaaacttggggcttcatgcaggtatgaagcagtaacactgccagagaatatcatggatatggatgtggagcagcccatgcttttctcagaggctgatactacaaggttccggggaatggtaatccctgtaatatgtatatttcggtggtgtctgattgtatgcattgaaagatggcattaagttatgagcaataaccacgagatagtcttgctttcagtctcctgacttttgccagattgtctctattcctctgaaatccgtcatgcatgtgcagcgtttggaggatttggatgaccaatacattaatgtcaacctagacgatgatgacttctcgcgcgctgagaatcatcaccaaggttttcatttcttttcttatgaattactctttgtggttgcttaagaggtgtactttctgactgaaatatgttatctcagctgatgcagaaaatatcaccctggctgataatttcgggtctgggcttggagagactgatgtgttcaatcgttttgagaggtgaagcaaaattttattcttctcttattctaccattcattttttgcacttcttgttccttattttggccagtacaattattcattgagcaatgttacaatgtcaccagattcgacataacagatgatgatgcaactttcaatgtcactcctgatggacacccacaggttccaagtaatctggttccttctccacctaggcaggaagactctcctcagcaacaagaaaaccatcatgctgcctcatcccctcttcacgaagaagctcaacaaggtcaattcaagcacatggtttggtctatttgatcctaaatttggaaagggtactatcatcctgtaaatcgggattttttttccagtttccatgccaaaagacaacaattctcatctctgttttttagcatgccacagatgcaaatgatatttcaaacaagtaaaacttccatccaaatatatctcagtgaatttatgatttgatgtagtttcttcagggatatgaacttgatggttatgtcaatatctcttaacagctagatattctatcttatcatatactctgctatattatgttggttaagaagctaaaagttcactcatcatctcctgttgcctttctacatttccaacggttggattttcgcgaggctaggtagagatgctgagcgattgccattgaatttgctacatgttatgtcattgaatttggcttttgtcaacgggataataaaacacattgtgtaacttgtcaccattcacactagtttactgactgaaattcaatttatattatatttgaatcttttctacttgctaaacaagacttgcatatagcctcttcctcgtgactatctgtacttcagtttgctatatctgatataagtgctggacatggtttgggtacattaatatgtgccatagtgcaagtgccttgttgcaattactgacaacttttctgcagggggggcatctgtaaaaaatgagcaagagcagcagaagatgaaggtttgtgatcaagcgagccgttagcttcaggaacatctagatgaagaagataattgttttgtttgatattgtttaaccagggtcagcaacctgctaaatcatcaaagagaaaaaaacgtaggaaagatgatgaggtgatgatggataacgaccagataatgatcccaggaaatgtatatcaaacatggctgaaggatccatcaagcctcattaccaaaaggcacagaatcaacagtgtatgtaattgctttgattacctaattctgtgtagagttttgtaactatgcagaattttgtgaacatatctgatgttttgccgtgtaatattgcagaaagttaatcttattcggtcaatcaagataagagacctcatggacttgcccctcgtttctctaatatcttccttggagaagtcacccttagaattttattatcctaaggaacttatgcagctttggaaggaatgtactgaagtcaagtccccaaaagctccatcttcaggttactacacaatttgtagttttctttcacctttctggatcaccaagggaactgtaagttttttataatttaatctgctaagttcgaactgaaaaggttgcaatcttttttaaaggagggcagcagtcatcatcaccagaacaacagcaaagaaacttgcctcctcaggcatttccaacccaggttatttctaaatttatatgtttatggaaatgatttatgctagcttttcctattgagctgtaacatttacattcattcttgaacttgtctgataagtggttcaatttgcagcctcaggttgataatgacagggaaatgggatttcacccagtggactttgcagatgacatcgaaaaactccgaggaaacactagtggggaatatggaagagattatgatgcttttcacagtgatcatagtgttactcctggaagtcctggtaagtacaaaaaacaatatattttatttatttagacactgaatgacatcagtcctgctgcagggctaagtcgcaggtctgcttcaagctctggtggctctggacggggatttacgcagttggatccagaagtacagttgccatccggaaggtgagtgttgattaatagcaaccttcactagttttctgaaatatgacttgttcctggaataaataaaaagttatccgtgcaaacttttgaagatgcatgtctttatactggagcctctttggtttatgttccaaagcatcgaagtggttagttgtcttattgatgaagtgttaggcaagaagaaaaggaaacggagattgttaccccccccctccagcggtaaccgctgaaaaccacgtgaaattcgtcctcaaatatttgaattaaaaatcggcggttttgcgttttcggcacagtaaccgcgatattcgcgcggttactgccaattgtgtagcagaatagaaacactcttaaaaaagtttaaaaaagcacttaaatgtgtgtagaaattggggataattagaagaatatataggatacttgcaaacctaacttttgggtgtaaaatacaaaaattctgcatgacattttctatgttatggacttcgaagcaattcaacatttcgtagcataataattcaacaacaacaattcaacaacctcacatttgaaagcatgtgccgagagagagcgatagagatagatgagaggacctaggagatatttttcccacttatgggacttaatgggccagataaacagtgcaactgtgcaacctaaggtccattacatttttcttttttccattttctaatgaacattcggttttaccctcaaatttgaatttatcttactctttttcaaaaaatttcttcactgtctaccactacaaatcaagaagtttttttatttgtttggaattttgaatttgggcaaaatttatcaaaccctaccaccggtaacccttactgtacccccgcggtaagcgcggttaccggtggtaagggaaaccctggtctaggctacttgactatcaactatcaagtggcagcttcctatctatggtccttctattttcatagaggtcttagtacaactgcgtgaggtcattgaacaggtccaagaggcagcattcatctggaaaaagctttgggaacctcgatccagttgaagaagaattcccattcgagcaagaacttagagatttcaagatgagaaggctttcagatgttgggccaactccaggttgacttcttttcagtccttatttcaaatgattctatacaaaagtgtggaaatttatttccccgtcatcataccgattaacttttaatttggtcacagacctgctggaagaaatcgaacctactcaaaccccatatgaaaagaaatccaatcctatcgaccaggtcacacaatcaatccactcgtaagtattcaactatacattatgtttgttccacagattacagtagatgtatactctgtgatcatcaaactaaatccatccaggtacctcaagctacactttgacaccccaggggcctcacagtctgaatcattaagtcagctagcacatgggatgactacagcaaaggctgcccgactcttctatcaagcatgcggtatgagttctataatcccaatgcgagtcaacagatgatgcaatcgctagagtaaacataatgttaacatttcatttgctggtttgatggttatttataatttccgtaagttcatttcaacacttgataagccatgtgtacttcctttccatattgcaactttgcaatgaaatattccaaccattgtttcaagttatgatatcaccgaaaccttttaggctcattacgtttttcccaggcttctctcattacattgaccaagtaaatataataagttcactttattagttcatgtagtgttaagggaggaaaacaaatgtgcaatagcactatttcctgagttggataagtgggcaacacctagtttgcttagccatagacagatgttggaaaatcttgaagaagtaccttggaaattcaatcgtacatcattgtggctatgtattttttgtttctttttcagattgtagccatgtttgatggacgagttagtgattttaactatgtcaataatggttatgtttcttttctccctcgatgagagaggatgagggcctaacaccatgtgtgggcaagatcggtgttcacaaaagtttggttagtgttttatattcttagctttactgtttagcgtggcagtttgatgtttttaggtgcttgtatgtgttattattggttcagtacttgtatttactgaggaccctttgttctgcagttttagcaactcatgattttatcaaggttaaccagctggaaccatacggagacatcttgatctcgaggggaccaaagatgtgacatcctaaatgtttaatagttggacaattttctgctgttgtggttcttgcacatctcgtgttatgtatggtgtttaagtttgcacatgaagtttagaaaaacttcgaaggaaggcttgtaggtacgttgtattggggcatgtatttaacactagttttgtgtaggaaagaaattttgtaagtatgctgaatggcttgatagctggtgcaaggttaaagtt&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062961.1 RefSeq:Os05g0580500]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Mmlin66