RiceWiki - User contributions [en]

Os03g0215200

2014-06-10T12:39:24Z

Sean: /* References */

Please input one-sentence summary here.

==Annotated Information==
===Function===

'' DROOPING LEAF (DL)''gene plays a regulatory role in the formation of rice carpel, flower meristem and development, while regulate the formation of rice leaf veins by promoting cell proliferation through the middle (Yamaguchi et al., 2004).
In the development of plants, related genes of tissue differentiation and organ development is regulated by time and space. ''DL'' is a member of the family YABBY, it promotes the formation and differentiation of leaf Mahogany leather veins. Consistent with these features, DL initial (presumably in the vein of position) and meristem presumed carpel position expressed in the central area of the leaf primordia.
The ''DL'' gene has an important function in rice development. Loss-of-function mutation of DL fails to form midrib, a strong structure formed in the central region of the leaf, resulting in a drooping leaf phenotype. ''DL'' encodes a putative transcription factor that belongs to the YABBY family, suggesting that it plays an essential role in these developmental processes by regulating many downstream genes, either directly or indirectly. (Abiko M et al., 2008)

===Expression===
Sequence analysis showed that, ''DL'' is one of the YABBY gene family. DL cDNA total length is 1037 bp, contains seven exons, and encoding a 194 amino acid protein product. The product contains two distinct domains: an amino-terminal zinc finger domain and a domain YABBY the carboxyl terminus. At the zinc finger domains, DL YABBY protein has similarity with other protein about 48.6 ~ 87.5%; and in the field of YABBY, DL protein has similarity with other YABBY proteins about 62.7 ~ 83.6%(Yamaguchi et al, 2004.). ''DL'' gene in rice small flower and leaf primordia skin-specific expression of the main veins, while being negative regulation by B genes such as the genes of SPW1(Nagasawa et al, 2003;. Yamaguchi et al, 2004.).

===Evolution===
Please input evolution information here.
''DL'' genes control the development of carpel rice flower and leaf veins, the gene abnormality causes leaf midrib and sheath hypoplasia, flowers have different degrees of deformity. Now this locus has found four recessive mutant alleles: dl-1, dl-2, dl-sup1 and dl-sup2.

==Labs working on this gene==
In order to clarify the mechanism of regulating expression of Os03g0215200, the authors identified cis-acting elements required for expression of this gene in time and space. The authors found that cis-acting elements in the vein of the leaf primordia assumed regional expression located in intron 2. Next they defined in the sequence of this region of about 200bp of the region and considered to be a conservative non-coding sequence, identified by phylogenetic blotting. Furthermore, some of the known sequences DG1, including a 5 'upstream region of about 7.4 kb intron 1 and 2, Os03g0215200 is proved sufficiently to cause expression of the assumption in the vein, and to suppress the expression of leaf primordia in the other regions, however it controls the expression of different heart Pete area is not located in DG1. The author use DG1 control the expressed sequence Os03g0215200-1 precisely, and then changed the morphology of rice: the resulting transgenic plants produced a blade at the end of the vein, this location is not in the vein of the wild type, and compared with the wild-type, it formed more upward the blade (Ohmori et al., 2011).

==References==
1. Yoshihiro Ohmori;Taiyo Toriba;Hidemitsu Nakamura;Hiroaki Ichikawa;Hiro-Yuki Hirano
Temporal and spatial regulation of DROOPING LEAF gene expression that promotes midrib formation in rice
The Plant Journal, 2011, 65(1): 77-86
2. Haifeng Li;Wanqi Liang;Yun Hua;Lu Zhu;Changsong Yin;Jie Xu;Ludovico Dreni;Martin M. Kater;Dabing Zhang
Rice MADS6 Interacts with the Floral Homeotic Genes SUPERWOMAN1, MADS3, MADS58, MADS13, and DROOPING LEAF in Specifying Floral Organ Identities and Meristem Fate
The Plant Cell, 2011, 23(7): 2536-2552
3. Haifeng Li;Wanqi Liang;Changsong Yin;Lu Zhu;Dabing Zhang
Genetic Interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in Specifying Rice Floral Organ Identities and Meristem Determinacy
Plant Physiology, 2011, 156(1): 263-274
4. Takahiro Yamaguchi;Nobuhiro Nagasawa;Shinji Kawasaki;Makoto Matsuoka;Yasuo Nagato;Hiro-Yuki Hirano
The YABBY Gene DROOPING LEAF Regulates Carpel Specification and Midrib Development in Oryza sativa
The Plant Cell, 2004, 16(2): 500-509
5. Nobuhiro Nagasawa;Masahiro Miyoshi;Yoshio Sano;Hikaru Satoh;Hiroyuki Hirano;Hajime Sakai;Yasuo Nagato
SUPERWOMAN1 and DROOPING LEAF genes control floral organ identity in rice
Development, 2003, 130(4): 705-718
6. Abiko M1, Ohmori Y, Hirano HY
Genome-wide expression profiling and identification of genes under the control of the DROOPING LEAF gene during midrib development in rice
Genes Genet Syst. 2008 Jun;83(3):237-44

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0215200|
Description = Drooping leaf protein|
Version = NM_001055910.1 GI:115451548 GeneID:4332058|
Length = 7428 bp|
Definition = Oryza sativa Japonica Group Os03g0215200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:6068581..6076008|
CDS = 6068682..6068750,6069637..6069774,6070763..6070856,6071155..6071203,6075032..6075107 ,6075308..6075400,6075628..6075693|
GCID = <gbrowseImage1>
name=NC_008396:6068581..6076008
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:6068581..6076008
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggatctcgtgtcgccgtccgagcacctgtgctacgtgcgctgcacctactgcaacaccgtgctcgcggttggagtcccatgcaagaggctgatggacaccgtgaccgtgaaatgtggccactgcaacaacctctccttcctcagcccgcggccgccgatggtgcagccgctctccccaactgatcaccccttgggcccgtttcagggaccttgcactgactgcaggaggaaccagccgctgccgctggtctcgccgacatcaaatgagggtagcccaagagcacccttcgttgtgaagcccccagagaagaaacaccgcctcccatctgcttacaaccgcttcatgagggaggaaatacagcgtatcaaagctgccaagccagatatccctcacagggaggccttcagcatggctgccaagaactgggcgaagtgcgacccccgctgctcatcgacggtttccacctccaacagcaaccccgagcccagagtagtagctgctcccattcctcatcaggagagggccaacgagcaggtggtcgagagcttcgacatcttcaagcagatggagcgcagcggctag</cdnaseq>|
AA = <aaseq>MDLVSPSEHLCYVRCTYCNTVLAVGVPCKRLMDTVTVKCGHCNN LSFLSPRPPMVQPLSPTDHPLGPFQGPCTDCRRNQPLPLVSPTSNEGSPRAPFVVKPP EKKHRLPSAYNRFMREEIQRIKAAKPDIPHREAFSMAAKNWAKCDPRCSSTVSTSNSN PEPRVVAAPIPHQERANEQVVESFDIFKQMERSG</aaseq>|
DNA = <dnaseqindica>102..170#1057..1194#2183..2276#2575..2623#6452..6527#6728..6820#7048..7113#ccccgcaccaccgctcacacttgctcctcctcctcctcctcctccgcctcagtgctagggctagcttgcttgtcgccgtcgccgccgtcgtcgccgccgcaatggatctcgtgtcgccgtccgagcacctgtgctacgtgcgctgcacctactgcaacaccgtgctcgcggtaaactcctcgagaaaccggagcagcggcttagcttcgcatgcagagaacgatcgatatatgctgctgctagctgcagctgcaccatggccttcgttctttggttatcttttgaattggacaagagagcaaagaacaaagtgttggttctgtctcgtgatctcctgctgctgcagattgcagagagggctctcactttggtttggtccaatcctttggcttgcttaatgtgcatcatggcatatgcttactctcacacaggacacagtgagagagagctagctagatagctgggaccaaatccagcttcttgcttgctctctctgtgtcctgaggagctagctgctaagctagtggaaacttccactggggattgttcatgacacgtgattgatgtgcccttgctgtcttcctgcatggtgttttattggctagctctctttttctttcttagataaaattcattggccatagctcttgatatgatgctttgtggtagaatgttctactcagctggttctctcgcagtgtcatgttccatctttcgcttccattatatccccttctttcattgttaccagctgcaggctgcatttttaaactggacaagaatccaatttagtagtacctgcatcttctgttcttccattaaaaaacaagtactactgaatcttctcttcttccttttctttttccctctctgttttgcatatcccactttatttgcttgctccatctagattccttccacacagatatgctgttctcctctactttttctccttaaggacagtgccatgcaacaaagagatgaaattaacgaagaaagtattggcttaattgcatgatgtgattaattaatttttcgtcttttgggtagctgcaggttggagtcccatgcaagaggctgatggacaccgtgaccgtgaaatgtggccactgcaacaacctctccttcctcagcccgcggccgccgatggtgcagccgctctccccaactgatcaccccttgggcccgtttcaggtcagtcctctcctctagctaattaagctagcgtttcattcttttctctctctctttctctgaatctccttgcagttcttgatatttttcctcctctttcttcttcttcttcttcgtcgtcttgatcttcttcagttctcgtgttgctgtttctagctactttctcttttctgcatatgggtagcttcttgcgaggagaaaggctggcgataaatctttctccttatacaagaaagggaagacatataattaaagaaattattaattgcacatggagagtgggacttttttttctcatgaaatatggtttagcagagctttggggtgttcttttgttcggtttctctctcctcttttcccttctctctctagctctctgtgtcctgtctcggctttatggagccactcagaatactgtttgcaaggcaagattaaacaaagctggattgcaaaagatttctctcttgcccatctgtggcctgggaaagcctcatgctgcaaaagcggccggccagttcatcttttttttttctcaggcagcatatgctgctagcagctgctctactgtggcattcatgcctctccaaattaaacctgcatttttatttatttacttctttttttttattttggtgtggaaagatgcattcattgcctgttttccgagttgactagtatagctacttctacccaagcagggagagagagagagagagagggagagagagagagagagagagagagagagggagagagagagagagagagagagagagagagagagagagagagagagagatctgagaaaatagttaggaaatgatgattttatcatcatccaactactactactactactactactactactactactactacactgatccatgcatggcaagcgtttgagagatcttgaaatgtgctagagctggaagctagtaattaaccttggttgcttcttgtgttggatatttcttggtgttagggaccttgcactgactgcaggaggaaccagccgctgccgctggtctcgccgacatcaaatgagggtagcccaagagcacccttcgttgtgaagcgtaagtagcaaagcaactatatgcacacttcacttgctatatcatacacgtgcatatataccagctagagtagatatcaatatctttctaattaaatttcttgcttgcttactgctcatttatatcagtcattcagtctctccttagtttcttgctaattgttaactgtcttcttctgacgagtccttcactagctctctgtgtcttgtgttcacatggaatatataaatatgaacaaacagctagctagcatatcttgtgtttcctctgacaaatgtttttatgtcttccattgtagccccagagaagaaacaccgcctcccatctgcttacaaccgcttcatgaggtgagaaactatttacctatttgtccatgcatttatcttatatatatgcttgtgatccctaatatggtttgcactcatgtgatcttatatatattttttctatttgttctgagtttttttttgagggagctagtagtggttcaatattttttttatttgtcctgtactataaactctatgagatctgcacctgttttgggagaaagtttcgattttattgaaggggatttatccccttctaaaagaaggaagaatatacataaaataatttatcatccaaggtcaggacatttttatattgtaaatctcagtacactaaataaaggctactatagctagtgtaattttgtgaattgaagaatattaattaatttgtttctgttaatcaaacaaaaacactctactccagtaatttacctagctagaccattgtgcatattatggatatctagcttgtttgtcattttgtggatattttcaaaaaaaaaaagaaataaagtgcatctatggtctgactttttttagggcatcgatctatggtctgatttaaaccttgtgatagcttgggggtttatacatggatatggtgaatgaattgtatatctaaaataaataccccattttacattttttttagttgccactatatatgttcttttctttaatatattttgttcttgttgatatatagtgaagggtctctctgtttccaggcttccatgtccagctgttttatttagctgagatctctgcatataattataatgtggtcctagttattgccaatttgcattgattttatcatatctctatctctctctacatatatgtatatgttgatatacacatttaacttagtttattatttaattaacacttatttttaacaaaagtacatttatttttgtttaatttattcctttccctgttaattgctcaggttttggaacagattccaggccatgatagtaataattaataaattagtaaacacattttcctttgaaaacacatgaccaatacacatctttattttttttgacaaaatacatctttatttaattcaatgagctgggcagtacgttcatcttgattcccaaataattttataaacacaagaaaaaaaacacttatatcatagttacccatatataactattaatgcctgtgtgcatcttatatctgatgcctgcagcctatgctagatatttatttacaatatctaaagcagttatgatggttaatttgtttcaacaaaatgaaagtaaaaaaaaagagttttggtgtttcctgttcacaccaacaatcctaatattttcatacattattcctgatatttgaactaccttttgtttgtgttatagaacgtaatcatcattattttttataaataactagtcaaagattatttttcaatatttacgacaaattattacatcaatacgtacgtgttacatatattttataaacaattggatacaattatgtttaatttcttcgatataaacactaagtataaatgctactccctccggcattacaagataattgagctattgccaccaaattattacaacaaaaaataatttgtagcaatatattgtactattgcaatgataatttttctgtggtaaaaaaatatattttattgcaatgatctacaattgttgttttatttagtacttttgttgcaatagaaaatgagatatcgccacaaaaaatcttactgttgcaaaaaaaatatgttctatcgccacaattttaatttttgtggcaatttacataataatggtacttttcactactttttgttgccacaatcagcaaattcgttgcaataaaaaaattgtcgtctcataatttaacttaatttttttcatatggatacggattgagacaaattttatatggaaattatagctctcgacgagatctataattttatagttgataacttgttcatatgaaatcttttagatattcaaatcttagttaggtaggctcaaatggaacaatatacatgggttcgaatcctagccaagacactgtataaagaaattaatagataggatgatagattaatatttgaacttttaatttggtacgtagaactaatgccacatatggagtggtggcaataatttttattacaacgatatgaagtgttgcaatatgattttttgccacatccattccgtggcaacatctaaatttattaccactaaataaaaattattgcaataacctattactacactatttattaccacggctagcaacgattttaa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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055910.1 RefSeq:Os03g0215200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

Os03g0215200

2014-06-10T12:37:59Z

Sean: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==
===Function===

'' DROOPING LEAF (DL)''gene plays a regulatory role in the formation of rice carpel, flower meristem and development, while regulate the formation of rice leaf veins by promoting cell proliferation through the middle (Yamaguchi et al., 2004).
In the development of plants, related genes of tissue differentiation and organ development is regulated by time and space. ''DL'' is a member of the family YABBY, it promotes the formation and differentiation of leaf Mahogany leather veins. Consistent with these features, DL initial (presumably in the vein of position) and meristem presumed carpel position expressed in the central area of the leaf primordia.
The ''DL'' gene has an important function in rice development. Loss-of-function mutation of DL fails to form midrib, a strong structure formed in the central region of the leaf, resulting in a drooping leaf phenotype. ''DL'' encodes a putative transcription factor that belongs to the YABBY family, suggesting that it plays an essential role in these developmental processes by regulating many downstream genes, either directly or indirectly. (Abiko M et al., 2008)

===Expression===
Sequence analysis showed that, ''DL'' is one of the YABBY gene family. DL cDNA total length is 1037 bp, contains seven exons, and encoding a 194 amino acid protein product. The product contains two distinct domains: an amino-terminal zinc finger domain and a domain YABBY the carboxyl terminus. At the zinc finger domains, DL YABBY protein has similarity with other protein about 48.6 ~ 87.5%; and in the field of YABBY, DL protein has similarity with other YABBY proteins about 62.7 ~ 83.6%(Yamaguchi et al, 2004.). ''DL'' gene in rice small flower and leaf primordia skin-specific expression of the main veins, while being negative regulation by B genes such as the genes of SPW1(Nagasawa et al, 2003;. Yamaguchi et al, 2004.).

===Evolution===
Please input evolution information here.
''DL'' genes control the development of carpel rice flower and leaf veins, the gene abnormality causes leaf midrib and sheath hypoplasia, flowers have different degrees of deformity. Now this locus has found four recessive mutant alleles: dl-1, dl-2, dl-sup1 and dl-sup2.

==Labs working on this gene==
In order to clarify the mechanism of regulating expression of Os03g0215200, the authors identified cis-acting elements required for expression of this gene in time and space. The authors found that cis-acting elements in the vein of the leaf primordia assumed regional expression located in intron 2. Next they defined in the sequence of this region of about 200bp of the region and considered to be a conservative non-coding sequence, identified by phylogenetic blotting. Furthermore, some of the known sequences DG1, including a 5 'upstream region of about 7.4 kb intron 1 and 2, Os03g0215200 is proved sufficiently to cause expression of the assumption in the vein, and to suppress the expression of leaf primordia in the other regions, however it controls the expression of different heart Pete area is not located in DG1. The author use DG1 control the expressed sequence Os03g0215200-1 precisely, and then changed the morphology of rice: the resulting transgenic plants produced a blade at the end of the vein, this location is not in the vein of the wild type, and compared with the wild-type, it formed more upward the blade (Ohmori et al., 2011).

==References==
1. Yoshihiro Ohmori;Taiyo Toriba;Hidemitsu Nakamura;Hiroaki Ichikawa;Hiro-Yuki Hirano
Temporal and spatial regulation of DROOPING LEAF gene expression that promotes midrib formation in rice
The Plant Journal, 2011, 65(1): 77-86
2. Haifeng Li;Wanqi Liang;Yun Hua;Lu Zhu;Changsong Yin;Jie Xu;Ludovico Dreni;Martin M. Kater;Dabing Zhang
Rice MADS6 Interacts with the Floral Homeotic Genes SUPERWOMAN1, MADS3, MADS58, MADS13, and DROOPING LEAF in Specifying Floral Organ Identities and Meristem Fate
The Plant Cell, 2011, 23(7): 2536-2552
3. Haifeng Li;Wanqi Liang;Changsong Yin;Lu Zhu;Dabing Zhang
Genetic Interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in Specifying Rice Floral Organ Identities and Meristem Determinacy
Plant Physiology, 2011, 156(1): 263-274
4. Takahiro Yamaguchi;Nobuhiro Nagasawa;Shinji Kawasaki;Makoto Matsuoka;Yasuo Nagato;Hiro-Yuki Hirano
The YABBY Gene DROOPING LEAF Regulates Carpel Specification and Midrib Development in Oryza sativa
The Plant Cell, 2004, 16(2): 500-509
5. Nobuhiro Nagasawa;Masahiro Miyoshi;Yoshio Sano;Hikaru Satoh;Hiroyuki Hirano;Hajime Sakai;Yasuo Nagato
SUPERWOMAN1 and DROOPING LEAF genes control floral organ identity in rice
Development, 2003, 130(4): 705-718

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0215200|
Description = Drooping leaf protein|
Version = NM_001055910.1 GI:115451548 GeneID:4332058|
Length = 7428 bp|
Definition = Oryza sativa Japonica Group Os03g0215200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:6068581..6076008|
CDS = 6068682..6068750,6069637..6069774,6070763..6070856,6071155..6071203,6075032..6075107 ,6075308..6075400,6075628..6075693|
GCID = <gbrowseImage1>
name=NC_008396:6068581..6076008
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:6068581..6076008
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggatctcgtgtcgccgtccgagcacctgtgctacgtgcgctgcacctactgcaacaccgtgctcgcggttggagtcccatgcaagaggctgatggacaccgtgaccgtgaaatgtggccactgcaacaacctctccttcctcagcccgcggccgccgatggtgcagccgctctccccaactgatcaccccttgggcccgtttcagggaccttgcactgactgcaggaggaaccagccgctgccgctggtctcgccgacatcaaatgagggtagcccaagagcacccttcgttgtgaagcccccagagaagaaacaccgcctcccatctgcttacaaccgcttcatgagggaggaaatacagcgtatcaaagctgccaagccagatatccctcacagggaggccttcagcatggctgccaagaactgggcgaagtgcgacccccgctgctcatcgacggtttccacctccaacagcaaccccgagcccagagtagtagctgctcccattcctcatcaggagagggccaacgagcaggtggtcgagagcttcgacatcttcaagcagatggagcgcagcggctag</cdnaseq>|
AA = <aaseq>MDLVSPSEHLCYVRCTYCNTVLAVGVPCKRLMDTVTVKCGHCNN LSFLSPRPPMVQPLSPTDHPLGPFQGPCTDCRRNQPLPLVSPTSNEGSPRAPFVVKPP EKKHRLPSAYNRFMREEIQRIKAAKPDIPHREAFSMAAKNWAKCDPRCSSTVSTSNSN PEPRVVAAPIPHQERANEQVVESFDIFKQMERSG</aaseq>|
DNA = <dnaseqindica>102..170#1057..1194#2183..2276#2575..2623#6452..6527#6728..6820#7048..7113#ccccgcaccaccgctcacacttgctcctcctcctcctcctcctccgcctcagtgctagggctagcttgcttgtcgccgtcgccgccgtcgtcgccgccgcaatggatctcgtgtcgccgtccgagcacctgtgctacgtgcgctgcacctactgcaacaccgtgctcgcggtaaactcctcgagaaaccggagcagcggcttagcttcgcatgcagagaacgatcgatatatgctgctgctagctgcagctgcaccatggccttcgttctttggttatcttttgaattggacaagagagcaaagaacaaagtgttggttctgtctcgtgatctcctgctgctgcagattgcagagagggctctcactttggtttggtccaatcctttggcttgcttaatgtgcatcatggcatatgcttactctcacacaggacacagtgagagagagctagctagatagctgggaccaaatccagcttcttgcttgctctctctgtgtcctgaggagctagctgctaagctagtggaaacttccactggggattgttcatgacacgtgattgatgtgcccttgctgtcttcctgcatggtgttttattggctagctctctttttctttcttagataaaattcattggccatagctcttgatatgatgctttgtggtagaatgttctactcagctggttctctcgcagtgtcatgttccatctttcgcttccattatatccccttctttcattgttaccagctgcaggctgcatttttaaactggacaagaatccaatttagtagtacctgcatcttctgttcttccattaaaaaacaagtactactgaatcttctcttcttccttttctttttccctctctgttttgcatatcccactttatttgcttgctccatctagattccttccacacagatatgctgttctcctctactttttctccttaaggacagtgccatgcaacaaagagatgaaattaacgaagaaagtattggcttaattgcatgatgtgattaattaatttttcgtcttttgggtagctgcaggttggagtcccatgcaagaggctgatggacaccgtgaccgtgaaatgtggccactgcaacaacctctccttcctcagcccgcggccgccgatggtgcagccgctctccccaactgatcaccccttgggcccgtttcaggtcagtcctctcctctagctaattaagctagcgtttcattcttttctctctctctttctctgaatctccttgcagttcttgatatttttcctcctctttcttcttcttcttcttcgtcgtcttgatcttcttcagttctcgtgttgctgtttctagctactttctcttttctgcatatgggtagcttcttgcgaggagaaaggctggcgataaatctttctccttatacaagaaagggaagacatataattaaagaaattattaattgcacatggagagtgggacttttttttctcatgaaatatggtttagcagagctttggggtgttcttttgttcggtttctctctcctcttttcccttctctctctagctctctgtgtcctgtctcggctttatggagccactcagaatactgtttgcaaggcaagattaaacaaagctggattgcaaaagatttctctcttgcccatctgtggcctgggaaagcctcatgctgcaaaagcggccggccagttcatcttttttttttctcaggcagcatatgctgctagcagctgctctactgtggcattcatgcctctccaaattaaacctgcatttttatttatttacttctttttttttattttggtgtggaaagatgcattcattgcctgttttccgagttgactagtatagctacttctacccaagcagggagagagagagagagagagggagagagagagagagagagagagagagagggagagagagagagagagagagagagagagagagagagagagagagagagatctgagaaaatagttaggaaatgatgattttatcatcatccaactactactactactactactactactactactactactacactgatccatgcatggcaagcgtttgagagatcttgaaatgtgctagagctggaagctagtaattaaccttggttgcttcttgtgttggatatttcttggtgttagggaccttgcactgactgcaggaggaaccagccgctgccgctggtctcgccgacatcaaatgagggtagcccaagagcacccttcgttgtgaagcgtaagtagcaaagcaactatatgcacacttcacttgctatatcatacacgtgcatatataccagctagagtagatatcaatatctttctaattaaatttcttgcttgcttactgctcatttatatcagtcattcagtctctccttagtttcttgctaattgttaactgtcttcttctgacgagtccttcactagctctctgtgtcttgtgttcacatggaatatataaatatgaacaaacagctagctagcatatcttgtgtttcctctgacaaatgtttttatgtcttccattgtagccccagagaagaaacaccgcctcccatctgcttacaaccgcttcatgaggtgagaaactatttacctatttgtccatgcatttatcttatatatatgcttgtgatccctaatatggtttgcactcatgtgatcttatatatattttttctatttgttctgagtttttttttgagggagctagtagtggttcaatattttttttatttgtcctgtactataaactctatgagatctgcacctgttttgggagaaagtttcgattttattgaaggggatttatccccttctaaaagaaggaagaatatacataaaataatttatcatccaaggtcaggacatttttatattgtaaatctcagtacactaaataaaggctactatagctagtgtaattttgtgaattgaagaatattaattaatttgtttctgttaatcaaacaaaaacactctactccagtaatttacctagctagaccattgtgcatattatggatatctagcttgtttgtcattttgtggatattttcaaaaaaaaaaagaaataaagtgcatctatggtctgactttttttagggcatcgatctatggtctgatttaaaccttgtgatagcttgggggtttatacatggatatggtgaatgaattgtatatctaaaataaataccccattttacattttttttagttgccactatatatgttcttttctttaatatattttgttcttgttgatatatagtgaagggtctctctgtttccaggcttccatgtccagctgttttatttagctgagatctctgcatataattataatgtggtcctagttattgccaatttgcattgattttatcatatctctatctctctctacatatatgtatatgttgatatacacatttaacttagtttattatttaattaacacttatttttaacaaaagtacatttatttttgtttaatttattcctttccctgttaattgctcaggttttggaacagattccaggccatgatagtaataattaataaattagtaaacacattttcctttgaaaacacatgaccaatacacatctttattttttttgacaaaatacatctttatttaattcaatgagctgggcagtacgttcatcttgattcccaaataattttataaacacaagaaaaaaaacacttatatcatagttacccatatataactattaatgcctgtgtgcatcttatatctgatgcctgcagcctatgctagatatttatttacaatatctaaagcagttatgatggttaatttgtttcaacaaaatgaaagtaaaaaaaaagagttttggtgtttcctgttcacaccaacaatcctaatattttcatacattattcctgatatttgaactaccttttgtttgtgttatagaacgtaatcatcattattttttataaataactagtcaaagattatttttcaatatttacgacaaattattacatcaatacgtacgtgttacatatattttataaacaattggatacaattatgtttaatttcttcgatataaacactaagtataaatgctactccctccggcattacaagataattgagctattgccaccaaattattacaacaaaaaataatttgtagcaatatattgtactattgcaatgataatttttctgtggtaaaaaaatatattttattgcaatgatctacaattgttgttttatttagtacttttgttgcaatagaaaatgagatatcgccacaaaaaatcttactgttgcaaaaaaaatatgttctatcgccacaattttaatttttgtggcaatttacataataatggtacttttcactactttttgttgccacaatcagcaaattcgttgcaataaaaaaattgtcgtctcataatttaacttaatttttttcatatggatacggattgagacaaattttatatggaaattatagctctcgacgagatctataattttatagttgataacttgttcatatgaaatcttttagatattcaaatcttagttaggtaggctcaaatggaacaatatacatgggttcgaatcctagccaagacactgtataaagaaattaatagataggatgatagattaatatttgaacttttaatttggtacgtagaactaatgccacatatggagtggtggcaataatttttattacaacgatatgaagtgttgcaatatgattttttgccacatccattccgtggcaacatctaaatttattaccactaaataaaaattattgcaataacctattactacactatttattaccacggctagcaacgattttaa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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055910.1 RefSeq:Os03g0215200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

Os03g0215200

2014-06-10T12:33:34Z

Sean: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==
===Function===

'' DROOPING LEAF''gene plays a regulatory role in the formation of rice carpel, flower meristem and development, while regulate the formation of rice leaf veins by promoting cell proliferation through the middle (Yamaguchi et al., 2004).
In the development of plants, related genes of tissue differentiation and organ development is regulated by time and space. ''DL'' is a member of the family YABBY, it promotes the formation and differentiation of leaf Mahogany leather veins. Consistent with these features, DL initial (presumably in the vein of position) and meristem presumed carpel position expressed in the central area of the leaf primordia.

===Expression===
Sequence analysis showed that, ''DL'' is one of the YABBY gene family. DL cDNA total length is 1037 bp, contains seven exons, and encoding a 194 amino acid protein product. The product contains two distinct domains: an amino-terminal zinc finger domain and a domain YABBY the carboxyl terminus. At the zinc finger domains, DL YABBY protein has similarity with other protein about 48.6 ~ 87.5%; and in the field of YABBY, DL protein has similarity with other YABBY proteins about 62.7 ~ 83.6%(Yamaguchi et al, 2004.). ''DL'' gene in rice small flower and leaf primordia skin-specific expression of the main veins, while being negative regulation by B genes such as the genes of SPW1(Nagasawa et al, 2003;. Yamaguchi et al, 2004.).

===Evolution===
Please input evolution information here.
''DL'' genes control the development of carpel rice flower and leaf veins, the gene abnormality causes leaf midrib and sheath hypoplasia, flowers have different degrees of deformity. Now this locus has found four recessive mutant alleles: dl-1, dl-2, dl-sup1 and dl-sup2.

==Labs working on this gene==
In order to clarify the mechanism of regulating expression of Os03g0215200, the authors identified cis-acting elements required for expression of this gene in time and space. The authors found that cis-acting elements in the vein of the leaf primordia assumed regional expression located in intron 2. Next they defined in the sequence of this region of about 200bp of the region and considered to be a conservative non-coding sequence, identified by phylogenetic blotting. Furthermore, some of the known sequences DG1, including a 5 'upstream region of about 7.4 kb intron 1 and 2, Os03g0215200 is proved sufficiently to cause expression of the assumption in the vein, and to suppress the expression of leaf primordia in the other regions, however it controls the expression of different heart Pete area is not located in DG1. The author use DG1 control the expressed sequence Os03g0215200-1 precisely, and then changed the morphology of rice: the resulting transgenic plants produced a blade at the end of the vein, this location is not in the vein of the wild type, and compared with the wild-type, it formed more upward the blade (Ohmori et al., 2011).

==References==
1. Yoshihiro Ohmori;Taiyo Toriba;Hidemitsu Nakamura;Hiroaki Ichikawa;Hiro-Yuki Hirano
Temporal and spatial regulation of DROOPING LEAF gene expression that promotes midrib formation in rice
The Plant Journal, 2011, 65(1): 77-86
2. Haifeng Li;Wanqi Liang;Yun Hua;Lu Zhu;Changsong Yin;Jie Xu;Ludovico Dreni;Martin M. Kater;Dabing Zhang
Rice MADS6 Interacts with the Floral Homeotic Genes SUPERWOMAN1, MADS3, MADS58, MADS13, and DROOPING LEAF in Specifying Floral Organ Identities and Meristem Fate
The Plant Cell, 2011, 23(7): 2536-2552
3. Haifeng Li;Wanqi Liang;Changsong Yin;Lu Zhu;Dabing Zhang
Genetic Interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in Specifying Rice Floral Organ Identities and Meristem Determinacy
Plant Physiology, 2011, 156(1): 263-274
4. Takahiro Yamaguchi;Nobuhiro Nagasawa;Shinji Kawasaki;Makoto Matsuoka;Yasuo Nagato;Hiro-Yuki Hirano
The YABBY Gene DROOPING LEAF Regulates Carpel Specification and Midrib Development in Oryza sativa
The Plant Cell, 2004, 16(2): 500-509
5. Nobuhiro Nagasawa;Masahiro Miyoshi;Yoshio Sano;Hikaru Satoh;Hiroyuki Hirano;Hajime Sakai;Yasuo Nagato
SUPERWOMAN1 and DROOPING LEAF genes control floral organ identity in rice
Development, 2003, 130(4): 705-718

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0215200|
Description = Drooping leaf protein|
Version = NM_001055910.1 GI:115451548 GeneID:4332058|
Length = 7428 bp|
Definition = Oryza sativa Japonica Group Os03g0215200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:6068581..6076008|
CDS = 6068682..6068750,6069637..6069774,6070763..6070856,6071155..6071203,6075032..6075107 ,6075308..6075400,6075628..6075693|
GCID = <gbrowseImage1>
name=NC_008396:6068581..6076008
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:6068581..6076008
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggatctcgtgtcgccgtccgagcacctgtgctacgtgcgctgcacctactgcaacaccgtgctcgcggttggagtcccatgcaagaggctgatggacaccgtgaccgtgaaatgtggccactgcaacaacctctccttcctcagcccgcggccgccgatggtgcagccgctctccccaactgatcaccccttgggcccgtttcagggaccttgcactgactgcaggaggaaccagccgctgccgctggtctcgccgacatcaaatgagggtagcccaagagcacccttcgttgtgaagcccccagagaagaaacaccgcctcccatctgcttacaaccgcttcatgagggaggaaatacagcgtatcaaagctgccaagccagatatccctcacagggaggccttcagcatggctgccaagaactgggcgaagtgcgacccccgctgctcatcgacggtttccacctccaacagcaaccccgagcccagagtagtagctgctcccattcctcatcaggagagggccaacgagcaggtggtcgagagcttcgacatcttcaagcagatggagcgcagcggctag</cdnaseq>|
AA = <aaseq>MDLVSPSEHLCYVRCTYCNTVLAVGVPCKRLMDTVTVKCGHCNN LSFLSPRPPMVQPLSPTDHPLGPFQGPCTDCRRNQPLPLVSPTSNEGSPRAPFVVKPP EKKHRLPSAYNRFMREEIQRIKAAKPDIPHREAFSMAAKNWAKCDPRCSSTVSTSNSN PEPRVVAAPIPHQERANEQVVESFDIFKQMERSG</aaseq>|
DNA = <dnaseqindica>102..170#1057..1194#2183..2276#2575..2623#6452..6527#6728..6820#7048..7113#ccccgcaccaccgctcacacttgctcctcctcctcctcctcctccgcctcagtgctagggctagcttgcttgtcgccgtcgccgccgtcgtcgccgccgcaatggatctcgtgtcgccgtccgagcacctgtgctacgtgcgctgcacctactgcaacaccgtgctcgcggtaaactcctcgagaaaccggagcagcggcttagcttcgcatgcagagaacgatcgatatatgctgctgctagctgcagctgcaccatggccttcgttctttggttatcttttgaattggacaagagagcaaagaacaaagtgttggttctgtctcgtgatctcctgctgctgcagattgcagagagggctctcactttggtttggtccaatcctttggcttgcttaatgtgcatcatggcatatgcttactctcacacaggacacagtgagagagagctagctagatagctgggaccaaatccagcttcttgcttgctctctctgtgtcctgaggagctagctgctaagctagtggaaacttccactggggattgttcatgacacgtgattgatgtgcccttgctgtcttcctgcatggtgttttattggctagctctctttttctttcttagataaaattcattggccatagctcttgatatgatgctttgtggtagaatgttctactcagctggttctctcgcagtgtcatgttccatctttcgcttccattatatccccttctttcattgttaccagctgcaggctgcatttttaaactggacaagaatccaatttagtagtacctgcatcttctgttcttccattaaaaaacaagtactactgaatcttctcttcttccttttctttttccctctctgttttgcatatcccactttatttgcttgctccatctagattccttccacacagatatgctgttctcctctactttttctccttaaggacagtgccatgcaacaaagagatgaaattaacgaagaaagtattggcttaattgcatgatgtgattaattaatttttcgtcttttgggtagctgcaggttggagtcccatgcaagaggctgatggacaccgtgaccgtgaaatgtggccactgcaacaacctctccttcctcagcccgcggccgccgatggtgcagccgctctccccaactgatcaccccttgggcccgtttcaggtcagtcctctcctctagctaattaagctagcgtttcattcttttctctctctctttctctgaatctccttgcagttcttgatatttttcctcctctttcttcttcttcttcttcgtcgtcttgatcttcttcagttctcgtgttgctgtttctagctactttctcttttctgcatatgggtagcttcttgcgaggagaaaggctggcgataaatctttctccttatacaagaaagggaagacatataattaaagaaattattaattgcacatggagagtgggacttttttttctcatgaaatatggtttagcagagctttggggtgttcttttgttcggtttctctctcctcttttcccttctctctctagctctctgtgtcctgtctcggctttatggagccactcagaatactgtttgcaaggcaagattaaacaaagctggattgcaaaagatttctctcttgcccatctgtggcctgggaaagcctcatgctgcaaaagcggccggccagttcatcttttttttttctcaggcagcatatgctgctagcagctgctctactgtggcattcatgcctctccaaattaaacctgcatttttatttatttacttctttttttttattttggtgtggaaagatgcattcattgcctgttttccgagttgactagtatagctacttctacccaagcagggagagagagagagagagagggagagagagagagagagagagagagagagggagagagagagagagagagagagagagagagagagagagagagagagagatctgagaaaatagttaggaaatgatgattttatcatcatccaactactactactactactactactactactactactactacactgatccatgcatggcaagcgtttgagagatcttgaaatgtgctagagctggaagctagtaattaaccttggttgcttcttgtgttggatatttcttggtgttagggaccttgcactgactgcaggaggaaccagccgctgccgctggtctcgccgacatcaaatgagggtagcccaagagcacccttcgttgtgaagcgtaagtagcaaagcaactatatgcacacttcacttgctatatcatacacgtgcatatataccagctagagtagatatcaatatctttctaattaaatttcttgcttgcttactgctcatttatatcagtcattcagtctctccttagtttcttgctaattgttaactgtcttcttctgacgagtccttcactagctctctgtgtcttgtgttcacatggaatatataaatatgaacaaacagctagctagcatatcttgtgtttcctctgacaaatgtttttatgtcttccattgtagccccagagaagaaacaccgcctcccatctgcttacaaccgcttcatgaggtgagaaactatttacctatttgtccatgcatttatcttatatatatgcttgtgatccctaatatggtttgcactcatgtgatcttatatatattttttctatttgttctgagtttttttttgagggagctagtagtggttcaatattttttttatttgtcctgtactataaactctatgagatctgcacctgttttgggagaaagtttcgattttattgaaggggatttatccccttctaaaagaaggaagaatatacataaaataatttatcatccaaggtcaggacatttttatattgtaaatctcagtacactaaataaaggctactatagctagtgtaattttgtgaattgaagaatattaattaatttgtttctgttaatcaaacaaaaacactctactccagtaatttacctagctagaccattgtgcatattatggatatctagcttgtttgtcattttgtggatattttcaaaaaaaaaaagaaataaagtgcatctatggtctgactttttttagggcatcgatctatggtctgatttaaaccttgtgatagcttgggggtttatacatggatatggtgaatgaattgtatatctaaaataaataccccattttacattttttttagttgccactatatatgttcttttctttaatatattttgttcttgttgatatatagtgaagggtctctctgtttccaggcttccatgtccagctgttttatttagctgagatctctgcatataattataatgtggtcctagttattgccaatttgcattgattttatcatatctctatctctctctacatatatgtatatgttgatatacacatttaacttagtttattatttaattaacacttatttttaacaaaagtacatttatttttgtttaatttattcctttccctgttaattgctcaggttttggaacagattccaggccatgatagtaataattaataaattagtaaacacattttcctttgaaaacacatgaccaatacacatctttattttttttgacaaaatacatctttatttaattcaatgagctgggcagtacgttcatcttgattcccaaataattttataaacacaagaaaaaaaacacttatatcatagttacccatatataactattaatgcctgtgtgcatcttatatctgatgcctgcagcctatgctagatatttatttacaatatctaaagcagttatgatggttaatttgtttcaacaaaatgaaagtaaaaaaaaagagttttggtgtttcctgttcacaccaacaatcctaatattttcatacattattcctgatatttgaactaccttttgtttgtgttatagaacgtaatcatcattattttttataaataactagtcaaagattatttttcaatatttacgacaaattattacatcaatacgtacgtgttacatatattttataaacaattggatacaattatgtttaatttcttcgatataaacactaagtataaatgctactccctccggcattacaagataattgagctattgccaccaaattattacaacaaaaaataatttgtagcaatatattgtactattgcaatgataatttttctgtggtaaaaaaatatattttattgcaatgatctacaattgttgttttatttagtacttttgttgcaatagaaaatgagatatcgccacaaaaaatcttactgttgcaaaaaaaatatgttctatcgccacaattttaatttttgtggcaatttacataataatggtacttttcactactttttgttgccacaatcagcaaattcgttgcaataaaaaaattgtcgtctcataatttaacttaatttttttcatatggatacggattgagacaaattttatatggaaattatagctctcgacgagatctataattttatagttgataacttgttcatatgaaatcttttagatattcaaatcttagttaggtaggctcaaatggaacaatatacatgggttcgaatcctagccaagacactgtataaagaaattaatagataggatgatagattaatatttgaacttttaatttggtacgtagaactaatgccacatatggagtggtggcaataatttttattacaacgatatgaagtgttgcaatatgattttttgccacatccattccgtggcaacatctaaatttattaccactaaataaaaattattgcaataacctattactacactatttattaccacggctagcaacgattttaa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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055910.1 RefSeq:Os03g0215200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

Os03g0215200

2014-06-10T12:31:44Z

Sean: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==
===Function===

''DL'' plays a regulatory role in the formation of rice carpel, flower meristem and development, while regulate the formation of rice leaf veins by promoting cell proliferation through the middle (Yamaguchi et al., 2004).
In the development of plants, related genes of tissue differentiation and organ development is regulated by time and space. ''DL'' is a member of the family YABBY, it promotes the formation and differentiation of leaf Mahogany leather veins. Consistent with these features, DL initial (presumably in the vein of position) and meristem presumed carpel position expressed in the central area of the leaf primordia.

===Expression===
Sequence analysis showed that, ''DL'' is one of the YABBY gene family. DL cDNA total length is 1037 bp, contains seven exons, and encoding a 194 amino acid protein product. The product contains two distinct domains: an amino-terminal zinc finger domain and a domain YABBY the carboxyl terminus. At the zinc finger domains, DL YABBY protein has similarity with other protein about 48.6 ~ 87.5%; and in the field of YABBY, DL protein has similarity with other YABBY proteins about 62.7 ~ 83.6%(Yamaguchi et al, 2004.). ''DL'' gene in rice small flower and leaf primordia skin-specific expression of the main veins, while being negative regulation by B genes such as the genes of SPW1(Nagasawa et al, 2003;. Yamaguchi et al, 2004.).

===Evolution===
Please input evolution information here.
''DL'' genes control the development of carpel rice flower and leaf veins, the gene abnormality causes leaf midrib and sheath hypoplasia, flowers have different degrees of deformity. Now this locus has found four recessive mutant alleles: dl-1, dl-2, dl-sup1 and dl-sup2.

==Labs working on this gene==
In order to clarify the mechanism of regulating expression of Os03g0215200, the authors identified cis-acting elements required for expression of this gene in time and space. The authors found that cis-acting elements in the vein of the leaf primordia assumed regional expression located in intron 2. Next they defined in the sequence of this region of about 200bp of the region and considered to be a conservative non-coding sequence, identified by phylogenetic blotting. Furthermore, some of the known sequences DG1, including a 5 'upstream region of about 7.4 kb intron 1 and 2, Os03g0215200 is proved sufficiently to cause expression of the assumption in the vein, and to suppress the expression of leaf primordia in the other regions, however it controls the expression of different heart Pete area is not located in DG1. The author use DG1 control the expressed sequence Os03g0215200-1 precisely, and then changed the morphology of rice: the resulting transgenic plants produced a blade at the end of the vein, this location is not in the vein of the wild type, and compared with the wild-type, it formed more upward the blade (Ohmori et al., 2011).

==References==
1. Yoshihiro Ohmori;Taiyo Toriba;Hidemitsu Nakamura;Hiroaki Ichikawa;Hiro-Yuki Hirano
Temporal and spatial regulation of DROOPING LEAF gene expression that promotes midrib formation in rice
The Plant Journal, 2011, 65(1): 77-86
2. Haifeng Li;Wanqi Liang;Yun Hua;Lu Zhu;Changsong Yin;Jie Xu;Ludovico Dreni;Martin M. Kater;Dabing Zhang
Rice MADS6 Interacts with the Floral Homeotic Genes SUPERWOMAN1, MADS3, MADS58, MADS13, and DROOPING LEAF in Specifying Floral Organ Identities and Meristem Fate
The Plant Cell, 2011, 23(7): 2536-2552
3. Haifeng Li;Wanqi Liang;Changsong Yin;Lu Zhu;Dabing Zhang
Genetic Interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in Specifying Rice Floral Organ Identities and Meristem Determinacy
Plant Physiology, 2011, 156(1): 263-274
4. Takahiro Yamaguchi;Nobuhiro Nagasawa;Shinji Kawasaki;Makoto Matsuoka;Yasuo Nagato;Hiro-Yuki Hirano
The YABBY Gene DROOPING LEAF Regulates Carpel Specification and Midrib Development in Oryza sativa
The Plant Cell, 2004, 16(2): 500-509
5. Nobuhiro Nagasawa;Masahiro Miyoshi;Yoshio Sano;Hikaru Satoh;Hiroyuki Hirano;Hajime Sakai;Yasuo Nagato
SUPERWOMAN1 and DROOPING LEAF genes control floral organ identity in rice
Development, 2003, 130(4): 705-718

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0215200|
Description = Drooping leaf protein|
Version = NM_001055910.1 GI:115451548 GeneID:4332058|
Length = 7428 bp|
Definition = Oryza sativa Japonica Group Os03g0215200, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:6068581..6076008|
CDS = 6068682..6068750,6069637..6069774,6070763..6070856,6071155..6071203,6075032..6075107 ,6075308..6075400,6075628..6075693|
GCID = <gbrowseImage1>
name=NC_008396:6068581..6076008
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:6068581..6076008
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggatctcgtgtcgccgtccgagcacctgtgctacgtgcgctgcacctactgcaacaccgtgctcgcggttggagtcccatgcaagaggctgatggacaccgtgaccgtgaaatgtggccactgcaacaacctctccttcctcagcccgcggccgccgatggtgcagccgctctccccaactgatcaccccttgggcccgtttcagggaccttgcactgactgcaggaggaaccagccgctgccgctggtctcgccgacatcaaatgagggtagcccaagagcacccttcgttgtgaagcccccagagaagaaacaccgcctcccatctgcttacaaccgcttcatgagggaggaaatacagcgtatcaaagctgccaagccagatatccctcacagggaggccttcagcatggctgccaagaactgggcgaagtgcgacccccgctgctcatcgacggtttccacctccaacagcaaccccgagcccagagtagtagctgctcccattcctcatcaggagagggccaacgagcaggtggtcgagagcttcgacatcttcaagcagatggagcgcagcggctag</cdnaseq>|
AA = <aaseq>MDLVSPSEHLCYVRCTYCNTVLAVGVPCKRLMDTVTVKCGHCNN LSFLSPRPPMVQPLSPTDHPLGPFQGPCTDCRRNQPLPLVSPTSNEGSPRAPFVVKPP EKKHRLPSAYNRFMREEIQRIKAAKPDIPHREAFSMAAKNWAKCDPRCSSTVSTSNSN PEPRVVAAPIPHQERANEQVVESFDIFKQMERSG</aaseq>|
DNA = <dnaseqindica>102..170#1057..1194#2183..2276#2575..2623#6452..6527#6728..6820#7048..7113#ccccgcaccaccgctcacacttgctcctcctcctcctcctcctccgcctcagtgctagggctagcttgcttgtcgccgtcgccgccgtcgtcgccgccgcaatggatctcgtgtcgccgtccgagcacctgtgctacgtgcgctgcacctactgcaacaccgtgctcgcggtaaactcctcgagaaaccggagcagcggcttagcttcgcatgcagagaacgatcgatatatgctgctgctagctgcagctgcaccatggccttcgttctttggttatcttttgaattggacaagagagcaaagaacaaagtgttggttctgtctcgtgatctcctgctgctgcagattgcagagagggctctcactttggtttggtccaatcctttggcttgcttaatgtgcatcatggcatatgcttactctcacacaggacacagtgagagagagctagctagatagctgggaccaaatccagcttcttgcttgctctctctgtgtcctgaggagctagctgctaagctagtggaaacttccactggggattgttcatgacacgtgattgatgtgcccttgctgtcttcctgcatggtgttttattggctagctctctttttctttcttagataaaattcattggccatagctcttgatatgatgctttgtggtagaatgttctactcagctggttctctcgcagtgtcatgttccatctttcgcttccattatatccccttctttcattgttaccagctgcaggctgcatttttaaactggacaagaatccaatttagtagtacctgcatcttctgttcttccattaaaaaacaagtactactgaatcttctcttcttccttttctttttccctctctgttttgcatatcccactttatttgcttgctccatctagattccttccacacagatatgctgttctcctctactttttctccttaaggacagtgccatgcaacaaagagatgaaattaacgaagaaagtattggcttaattgcatgatgtgattaattaatttttcgtcttttgggtagctgcaggttggagtcccatgcaagaggctgatggacaccgtgaccgtgaaatgtggccactgcaacaacctctccttcctcagcccgcggccgccgatggtgcagccgctctccccaactgatcaccccttgggcccgtttcaggtcagtcctctcctctagctaattaagctagcgtttcattcttttctctctctctttctctgaatctccttgcagttcttgatatttttcctcctctttcttcttcttcttcttcgtcgtcttgatcttcttcagttctcgtgttgctgtttctagctactttctcttttctgcatatgggtagcttcttgcgaggagaaaggctggcgataaatctttctccttatacaagaaagggaagacatataattaaagaaattattaattgcacatggagagtgggacttttttttctcatgaaatatggtttagcagagctttggggtgttcttttgttcggtttctctctcctcttttcccttctctctctagctctctgtgtcctgtctcggctttatggagccactcagaatactgtttgcaaggcaagattaaacaaagctggattgcaaaagatttctctcttgcccatctgtggcctgggaaagcctcatgctgcaaaagcggccggccagttcatcttttttttttctcaggcagcatatgctgctagcagctgctctactgtggcattcatgcctctccaaattaaacctgcatttttatttatttacttctttttttttattttggtgtggaaagatgcattcattgcctgttttccgagttgactagtatagctacttctacccaagcagggagagagagagagagagagggagagagagagagagagagagagagagagggagagagagagagagagagagagagagagagagagagagagagagagagatctgagaaaatagttaggaaatgatgattttatcatcatccaactactactactactactactactactactactactactacactgatccatgcatggcaagcgtttgagagatcttgaaatgtgctagagctggaagctagtaattaaccttggttgcttcttgtgttggatatttcttggtgttagggaccttgcactgactgcaggaggaaccagccgctgccgctggtctcgccgacatcaaatgagggtagcccaagagcacccttcgttgtgaagcgtaagtagcaaagcaactatatgcacacttcacttgctatatcatacacgtgcatatataccagctagagtagatatcaatatctttctaattaaatttcttgcttgcttactgctcatttatatcagtcattcagtctctccttagtttcttgctaattgttaactgtcttcttctgacgagtccttcactagctctctgtgtcttgtgttcacatggaatatataaatatgaacaaacagctagctagcatatcttgtgtttcctctgacaaatgtttttatgtcttccattgtagccccagagaagaaacaccgcctcccatctgcttacaaccgcttcatgaggtgagaaactatttacctatttgtccatgcatttatcttatatatatgcttgtgatccctaatatggtttgcactcatgtgatcttatatatattttttctatttgttctgagtttttttttgagggagctagtagtggttcaatattttttttatttgtcctgtactataaactctatgagatctgcacctgttttgggagaaagtttcgattttattgaaggggatttatccccttctaaaagaaggaagaatatacataaaataatttatcatccaaggtcaggacatttttatattgtaaatctcagtacactaaataaaggctactatagctagtgtaattttgtgaattgaagaatattaattaatttgtttctgttaatcaaacaaaaacactctactccagtaatttacctagctagaccattgtgcatattatggatatctagcttgtttgtcattttgtggatattttcaaaaaaaaaaagaaataaagtgcatctatggtctgactttttttagggcatcgatctatggtctgatttaaaccttgtgatagcttgggggtttatacatggatatggtgaatgaattgtatatctaaaataaataccccattttacattttttttagttgccactatatatgttcttttctttaatatattttgttcttgttgatatatagtgaagggtctctctgtttccaggcttccatgtccagctgttttatttagctgagatctctgcatataattataatgtggtcctagttattgccaatttgcattgattttatcatatctctatctctctctacatatatgtatatgttgatatacacatttaacttagtttattatttaattaacacttatttttaacaaaagtacatttatttttgtttaatttattcctttccctgttaattgctcaggttttggaacagattccaggccatgatagtaataattaataaattagtaaacacattttcctttgaaaacacatgaccaatacacatctttattttttttgacaaaatacatctttatttaattcaatgagctgggcagtacgttcatcttgattcccaaataattttataaacacaagaaaaaaaacacttatatcatagttacccatatataactattaatgcctgtgtgcatcttatatctgatgcctgcagcctatgctagatatttatttacaatatctaaagcagttatgatggttaatttgtttcaacaaaatgaaagtaaaaaaaaagagttttggtgtttcctgttcacaccaacaatcctaatattttcatacattattcctgatatttgaactaccttttgtttgtgttatagaacgtaatcatcattattttttataaataactagtcaaagattatttttcaatatttacgacaaattattacatcaatacgtacgtgttacatatattttataaacaattggatacaattatgtttaatttcttcgatataaacactaagtataaatgctactccctccggcattacaagataattgagctattgccaccaaattattacaacaaaaaataatttgtagcaatatattgtactattgcaatgataatttttctgtggtaaaaaaatatattttattgcaatgatctacaattgttgttttatttagtacttttgttgcaatagaaaatgagatatcgccacaaaaaatcttactgttgcaaaaaaaatatgttctatcgccacaattttaatttttgtggcaatttacataataatggtacttttcactactttttgttgccacaatcagcaaattcgttgcaataaaaaaattgtcgtctcataatttaacttaatttttttcatatggatacggattgagacaaattttatatggaaattatagctctcgacgagatctataattttatagttgataacttgttcatatgaaatcttttagatattcaaatcttagttaggtaggctcaaatggaacaatatacatgggttcgaatcctagccaagacactgtataaagaaattaatagataggatgatagattaatatttgaacttttaatttggtacgtagaactaatgccacatatggagtggtggcaataatttttattacaacgatatgaagtgttgcaatatgattttttgccacatccattccgtggcaacatctaaatttattaccactaaataaaaattattgcaataacctattactacactatttattaccacggctagcaacgattttaaaattgtggcaatatgtatctattgccacaattttagtattgatgccacgatttttttcgtggcaacaaataatttttctagtagtacgggttgataatacttgtcgttttggataagggcacggtcttcaaaaaaacaactttgaccattattttctatcataatatgtaaaaaaatattaacaaatatatgatcttattaaagtacttttaaagactaatctatacatgtggtcaccatatttgaaaaacaaatattttgaaaatgattcataatcaaagattttaaagtttgacctcacccttgtccaaaacgacaagtattatcagcccagagggagtatccttttctgaaattttttaactaaattaaacacacttaatttaaaagtagtttcacctaccaacctacctgctataagtaacacacacttgttgatgttaaattcctgataatgcaagccatcttgccttttaagtgttcattgttttgttgttagaaattactaacgtaactgtaaaagatggggctgttaatttgtgcgcccttgtagtataacagtttattaaaaaaactagatcattttgaataaaagacattaatttatatgtgccatcttaacgtgtacaacttatggaagtttcagcatgctcttagatgcccatccatgggatagttttaattaaattaaccgacccttccataaaatagtgggtgctataaatttaagttaatttgatactgcattgctactagattgaccatttctttaattaaatactaattgagttagtaaaccaattatttcactaaaactgaataagcctaggaccaatctatatatttttatggtgaaagttggatgcaggggaaatttcacagattcgtggaaaattaaaatgggacaatatactagcttgaacacaaataagtagtatcaaaaggtagaacatatgatatattagcaaaacaccacacatgcattattatccaatgtgtgcgagctaattattgtacatatgctaatgctactgtaatataaatcacatttagtactccctccgtcctagaatataagaagttttagagttgaacacggttattaagaaagtaggtagaagttagtggtggagggttgtgattggatgagtagtggagataggtgggaaaagtgaatggtggaagtttgtgattgattggaaagagaatgttggtggagaagttgttatattttaggataaattctaagagctgaaaattattatattttgagatggagagagtatcaattaatctttttacagacccgtacttcgatatatcatcgatgaagtaattaaccaaagggaataaaatatgcatgggacaactaaactgggatatatatgtagacaatgccagtttatatctaatcgacacaaccatatgcatatatgcattgcatgtacctacgtacgtactacactctcttctcagaaataaatctgcacatggatattttagcggatcaattaattataattaatatatgatgatgcagggaggaaatacagcgtatcaaagctgccaagccagatatccctcacagggaggccttcagcatggctgccaagaacgtaagctctactcatccccaatatctcccggcccccaatttgcatgtgataattgtgacagttattagctataatcaaccgatccatcggtctctagatctctcgcaccttgcttacacatcaattactagtacaataatattaattacttgcaccgatggagatgcaagtgtctgatgatcagctctgcatttatgcagtgggcgaagtgcgacccccgctgctcatcgacggtttccacctccaacagcaaccccgagcccagagtagtagctgctcccattcctcatcaggtgatgatcatattgacgataattaacttgccatagcaacagagacaccgatcatatatacatacacacaaaatgagagtataattaaggcagtttctctatatgatttgatttatttgatgcatggccaccacaaaacgattatgattttagatagttcttgtgtgatatgatctcaccgtggaattgttctttaattatgtgctatattattgatgttatatcaggagagggccaacgagcaggtggtcgagagcttcgacatcttcaagcagatggagcgcagcggctagggcggcggcggcggccggagccggcggcgatctatatcggcggtgaagctcgtatgaagctagctagcctgcaggccggccactggggagagtaccaaatttcagatcccccttattatcaccgtcgtcagctcagctcatgcatgcatgctcatcgttcccctttagcatatatctgtgctcgttttgtgtttattagttaattatgtttgatcttgttaatttgttgttgcatggagtatgtaccccctataagacccagctgctgctaccgtacgatatacgtacgtatgctatatatatatatatatatat</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055910.1 RefSeq:Os03g0215200]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

Os03g0285700

2014-06-10T12:10:30Z

Sean: /* Function */

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsAPX1 is a member of ascorbate peroxidase (APX) family. It is a haeme-containing enzyme of the ascorbate glutathione cycle that detoxifies reactive oxygen species in plants by catalyzing the conversion of hydrogen peroxide to water using ascorbate as a specific electron donor. The OsAPX1 expression is stress regulated[1].
OsAPX1 can affect the ability of rice plants to withstand chilling at the booting stage. When overexpress OsAPX1 in rice, it can increase APX activity which determine the antioxidant capacity. The levels of H2O2 and the MDA content increased by 1.5-fold and twofold, respectively in WT plants subjected to a 12℃ treatment for six days. In contrast, transgenic lines showed small changes in H2O2 levels and MDA content under cold stress, and H2O2 levels and MDA content were significantly lower than in WT plants. And the spikelet fertility was significantly higher in transgenic lines than in WT plants after a 12℃ treatment for six days. The reason is the APX activity enhances H2O2-scavenging capacity and protects spikelets, thereby increasing spikelet fertility under cold stress[2].

H2O2 may be involved in the regulation of HS- and Cd-increased APX and GR activities in leaves of rice seedlings.[4]

===Expression===
Different APX isoforms are present in discrete subcellular compartments in rice and their expression is stress regulated.And OsAPX1 exist in the cytoplasm of rice.
OsAPX1 show a weak constitutive expression in leaves. Their transcripts were up-regulated upon wounding (by cut), and diverse signals such as salicylic acid (SA), ethylene (using the ethylene generator, ethephon), abscisic acid (ABA), hydrogen peroxide, copper sulfate, protein phosphatase (PP) inhibitors, cantharidin (CN), endothall (EN) and okadaic acid (OA), and blast pathogen (Magnaporthe grisea) attack, but surprisingly not by jasmonic acid (JA).
OsAPX1 mRNAs expression manifested a clear rhythmicity under light/dark cycle[3].

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
[1]Saurabh Pandey; Yogesh Kumar Negi,et al.Modeling and phylogenetic analysis of cytosolic ascorbate peroxidase (OsAPX1) from rice reveal signature motifs that may play a role in stress tolerance.Bioinformation,2014,10(3):119-123

[2]Yutaka Sato;Yukari Masuta et al.Enhanced chilling tolerance at the booting stage in rice by transgenic overexpression of the ascorbate peroxidase gene, OsAPXa.Plant Cell Reports, 2011, 30(3): 399-406.

[3]Ganesh Kumar Agrawal, Nam-Soo Jwac et al.Importance of ascorbate peroxidases OsAPX1 and OsAPX2 in the rice pathogen response pathways and growth and reproduction revealed by their transcriptional profiling.Gene, 2003, 322: 93-103.

[4]Ting-Shao Chou;Yun-Yang Chao;Ching Huei Kao
Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings
Journal of Plant Physiology, 2012, 169(5): 478-486

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0285700|
Description = Similar to L-ascorbate peroxidase|
Version = NM_001056304.1 GI:115452336 GeneID:4332474|
Length = 3335 bp|
Definition = Oryza sativa Japonica Group Os03g0285700, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:9893999..9897333|
CDS = 9894067..9894185,9894281..9894455,9894550..9894615,9895972..9896020,9896354..9896439 ,9896526..9896605,9896701..9896803,9896921..9896979,9897066..9897081 |
GCID = <gbrowseImage1>
name=NC_008396:9893999..9897333
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:9893999..9897333
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctaagaactaccccgtcgtgagcgccgagtaccaggaggccgtcgagaaggccaggcagaagctgcgcgccctcatcgccgagaagagctgcgcccctctcatgctccgcctcgcgtggcactcggcggggacgttcgacgtgtcgtcgaagaccgggggcccgttcgggacgatgaagaccccggcggagctgtcgcacgccgccaacgcggggctggacatcgcggtgcggatgctcgagcccatcaaggaggagatacccaccatctcctacgccgatttctaccagcttgccggagttgtggccgtcgaggtgtccggtggacctgccgtccccttccacccaggaagggaggacaaacctgcacccccacctgagggccgtcttcctgatgctaccaagggttctgaccacctaaggcaggtcttcggtgcgcagatgggcttgagtgatcaggacattgttgccctctctggcggtcacaccctgggaaggtgccacaaggaaagatctggttttgagggaccttggacaagaaaccctctgcagtttgacaactcttacttcacggagcttctgagtggtgacaaggagggccttcttcagcttcctagtgacaaagccctgctgagtgaccctgccttccgcccactcgtcgagaaatatgctgcagatgagaaggctttctttgaagactacaaggaggcccacctcaagctctccgaactggggttcgctgatgcttaa</cdnaseq>|
AA = <aaseq>MAKNYPVVSAEYQEAVEKARQKLRALIAEKSCAPLMLRLAWHSA GTFDVSSKTGGPFGTMKTPAELSHAANAGLDIAVRMLEPIKEEIPTISYADFYQLAGV VAVEVSGGPAVPFHPGREDKPAPPPEGRLPDATKGSDHLRQVFGAQMGLSDQDIVALS GGHTLGRCHKERSGFEGPWTRNPLQFDNSYFTELLSGDKEGLLQLPSDKALLSDPAFR PLVEKYAADEKAFFEDYKEAHLKLSELGFADA</aaseq>|
DNA = <dnaseqindica>69..187#283..457#552..617#1974..2022#2356..2441#2528..2607#2703..2805#2923..2981#3068..3083#aagcctttcgagtcgtcttctccccttcttctcctcctcctcctcgattcggagctccacccgcagccatggctaagaactaccccgtcgtgagcgccgagtaccaggaggccgtcgagaaggccaggcagaagctgcgcgccctcatcgccgagaagagctgcgcccctctcatgctccgcctcgcgtaagccgtcgccctcgcactcgctcgcttctaggttttgtgtagaggcggatttccgtagactgatccggtttttgtggagacggatcgcgcaggtggcactcggcggggacgttcgacgtgtcgtcgaagaccgggggcccgttcgggacgatgaagaccccggcggagctgtcgcacgccgccaacgcggggctggacatcgcggtgcggatgctcgagcccatcaaggaggagatacccaccatctcctacgccgatttctaccaggttcgccgcgagccccggatagatctggatggacggccccgatctggtcaaacggacgtggttctgatgttttttccccgttgtttttgcgcagcttgccggagttgtggccgtcgaggtgtccggtggacctgccgtccccttccacccaggaagggaggtgagatttcctattacctttcaactcttggggtttttctcagggttctagttaggccttgatatggcagtggtaaactggatggcgtgtggatttgtttgtgatgccacgagattagtcgtaatttgttagtgctccattgatgtgtgtgcttttcatttgtggggactgggggttggtggttcctagagagtagacaaagtgatcaccactaatctaaggccagcagtataataatattttaacagtacggacttttctatattatatacccggataataccgtcaaattgggtttgttcgtattcttgttggtgggtcggcgttacttgacatcctctagatctagaggtggttaaatatccgtgacctcatttacatttccgtaagaccttgtttatttaccataaacatgctaattgttattttttagttattgttggctcatgtgtttgtgtcagttgagtcacataggattcattttcatgcatgctgtatggtgtgaagtttgttatccattcatgcacaaggatattatttttaaggatgtcgccttatcctttttctttgtagtctggctattctcaaagtagtcactgatttaccatggttgtttgcggcatttccctgttcagggttttgatagttactgatctttcattttatcatttttattttttactcaggatagtagttctcatcctaatcgatgtcatactctaaaccatgacaaataaacaattattttctgaattaatggaagaagccttggggacctggaagaagccttggggaccggggttgtttccgttctctatgcatgatatagtttgatctgttggattgtgccatgtaggatatttgttgtgctttgtgaatataaacttaagaaagcatccattatttacaagtctagttggttcagataaagtatattagggcctcccaacttattgcattgacataaaatgataattaggggttcaatctgaaggcagttggaaagcttaatgaaaaaacagtgggccgatatatatctatatgttatgttttaggaaactttagaaaggtgacttggtgcttgttttaggatataataactgttatggatatatctttttttataaatacataatgttcagtaattattatgcaccttcaacaatatcaccgatgatagtaatatatatcatatatgtaacattatacagtgcttaaaatttgcttgtgcatatctggctcatctttatatttatttctaaaatggagagatgattaagtgtttttttatgttccattttatattcattgtgttgttaattgagattgttttcatccttccttgaccttttgcaggacaaacctgcacccccacctgagggccgtcttcctgatgctaccaagggtatgttgccagtttacactactagtctcttccaggaactatagaaacattcaccactattgccataaaagaaacccttgcaaataagtcatcctttaaaatgtttttgtttctgcaatagttgaggtgttatttctgtgtagcacatctaaaatgcttagttatggtagaagtttttcccactgcacacttgacaaatgattcctgtgttgttgtaggcatgttgtatttttctgcatgcttcatttctgatatgatcctgctttgaccttgttctagtaaacctcatgtaaagcaccattgcttacactttgagcttgattgctttaccaggttctgaccacctaaggcaggtcttcggtgcgcagatgggcttgagtgatcaggacattgttgccctctctggcggtcacaccctggttagtttggctattaaccattgtatgcctctgcagtatgtaggagtacatgttctaatgtcggatttctgtgctttgttctgcagggaaggtgccacaaggaaagatctggttttgagggaccttggacaagaaaccctctgcagtttgacaactcttacttcacgtaagcgatgttacgagtggttgaattttgttcttatgctgaacaaagttggtaacaaaaatgttgaactcatctctccgcgacgattttaatagggagcttctgagtggtgacaaggagggccttcttcagcttcctagtgacaaagccctgctgagtgaccctgccttccgcccactcgtcgagaaatatgctgcagtatgtctcctatcaatcatcatcttgtgttccacgttttgcatcttgtgttcctcgttttgcagagatatgccagaacatgatactgattgtgttttttttccccaattctgctaggatgagaaggctttctttgaagactacaaggaggcccacctcaagctctccgaactggggtgagtaatggtttgtcaatccatttatcgtgttatctttcatgacaacaaggtcatggtagctaattgtactcttgtgatttcaggttcgctgatgcttaagaggtttctagtctactactgctagtacattgcccgtggtactcttgttttgcatctaggctagggccagtgtgaaccagcagactaccactgtgagtcgcctctgtaataaaatttgttcggcctatggccatcctcagtacgttgtcaatgtctcggtgggctgttatgctcaactgcaatgctgcatccactgaaacctctttctagatgtgttggtatgaaatgcggtattgcgttcccgatttcccc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001056304.1 RefSeq:Os03g0285700]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

Os03g0285700

2014-06-10T12:08:56Z

Sean: /* Function */

Please input one-sentence summary here.

==Annotated Information==
===Function===
OsAPX1 is a member of ascorbate peroxidase (APX) family. It is a haeme-containing enzyme of the ascorbate glutathione cycle that detoxifies reactive oxygen species in plants by catalyzing the conversion of hydrogen peroxide to water using ascorbate as a specific electron donor. The OsAPX1 expression is stress regulated[1].
OsAPX1 can affect the ability of rice plants to withstand chilling at the booting stage. When overexpress OsAPX1 in rice, it can increase APX activity which determine the antioxidant capacity. The levels of H2O2 and the MDA content increased by 1.5-fold and twofold, respectively in WT plants subjected to a 12℃ treatment for six days. In contrast, transgenic lines showed small changes in H2O2 levels and MDA content under cold stress, and H2O2 levels and MDA content were significantly lower than in WT plants. And the spikelet fertility was significantly higher in transgenic lines than in WT plants after a 12℃ treatment for six days. The reason is the APX activity enhances H2O2-scavenging capacity and protects spikelets, thereby increasing spikelet fertility under cold stress[2].

H2O2 may be involved in the regulation of HS- and Cd-increased APX and GR activities in leaves of rice seedlings.[4]

===Expression===
Different APX isoforms are present in discrete subcellular compartments in rice and their expression is stress regulated.And OsAPX1 exist in the cytoplasm of rice.
OsAPX1 show a weak constitutive expression in leaves. Their transcripts were up-regulated upon wounding (by cut), and diverse signals such as salicylic acid (SA), ethylene (using the ethylene generator, ethephon), abscisic acid (ABA), hydrogen peroxide, copper sulfate, protein phosphatase (PP) inhibitors, cantharidin (CN), endothall (EN) and okadaic acid (OA), and blast pathogen (Magnaporthe grisea) attack, but surprisingly not by jasmonic acid (JA).
OsAPX1 mRNAs expression manifested a clear rhythmicity under light/dark cycle[3].

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
[1]Saurabh Pandey; Yogesh Kumar Negi,et al.Modeling and phylogenetic analysis of cytosolic ascorbate peroxidase (OsAPX1) from rice reveal signature motifs that may play a role in stress tolerance.Bioinformation,2014,10(3):119-123

[2]Yutaka Sato;Yukari Masuta et al.Enhanced chilling tolerance at the booting stage in rice by transgenic overexpression of the ascorbate peroxidase gene, OsAPXa.Plant Cell Reports, 2011, 30(3): 399-406.

[3]Ganesh Kumar Agrawal, Nam-Soo Jwac et al.Importance of ascorbate peroxidases OsAPX1 and OsAPX2 in the rice pathogen response pathways and growth and reproduction revealed by their transcriptional profiling.Gene, 2003, 322: 93-103.

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0285700|
Description = Similar to L-ascorbate peroxidase|
Version = NM_001056304.1 GI:115452336 GeneID:4332474|
Length = 3335 bp|
Definition = Oryza sativa Japonica Group Os03g0285700, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:9893999..9897333|
CDS = 9894067..9894185,9894281..9894455,9894550..9894615,9895972..9896020,9896354..9896439 ,9896526..9896605,9896701..9896803,9896921..9896979,9897066..9897081 |
GCID = <gbrowseImage1>
name=NC_008396:9893999..9897333
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:9893999..9897333
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctaagaactaccccgtcgtgagcgccgagtaccaggaggccgtcgagaaggccaggcagaagctgcgcgccctcatcgccgagaagagctgcgcccctctcatgctccgcctcgcgtggcactcggcggggacgttcgacgtgtcgtcgaagaccgggggcccgttcgggacgatgaagaccccggcggagctgtcgcacgccgccaacgcggggctggacatcgcggtgcggatgctcgagcccatcaaggaggagatacccaccatctcctacgccgatttctaccagcttgccggagttgtggccgtcgaggtgtccggtggacctgccgtccccttccacccaggaagggaggacaaacctgcacccccacctgagggccgtcttcctgatgctaccaagggttctgaccacctaaggcaggtcttcggtgcgcagatgggcttgagtgatcaggacattgttgccctctctggcggtcacaccctgggaaggtgccacaaggaaagatctggttttgagggaccttggacaagaaaccctctgcagtttgacaactcttacttcacggagcttctgagtggtgacaaggagggccttcttcagcttcctagtgacaaagccctgctgagtgaccctgccttccgcccactcgtcgagaaatatgctgcagatgagaaggctttctttgaagactacaaggaggcccacctcaagctctccgaactggggttcgctgatgcttaa</cdnaseq>|
AA = <aaseq>MAKNYPVVSAEYQEAVEKARQKLRALIAEKSCAPLMLRLAWHSA GTFDVSSKTGGPFGTMKTPAELSHAANAGLDIAVRMLEPIKEEIPTISYADFYQLAGV VAVEVSGGPAVPFHPGREDKPAPPPEGRLPDATKGSDHLRQVFGAQMGLSDQDIVALS GGHTLGRCHKERSGFEGPWTRNPLQFDNSYFTELLSGDKEGLLQLPSDKALLSDPAFR PLVEKYAADEKAFFEDYKEAHLKLSELGFADA</aaseq>|
DNA = <dnaseqindica>69..187#283..457#552..617#1974..2022#2356..2441#2528..2607#2703..2805#2923..2981#3068..3083#aagcctttcgagtcgtcttctccccttcttctcctcctcctcctcgattcggagctccacccgcagccatggctaagaactaccccgtcgtgagcgccgagtaccaggaggccgtcgagaaggccaggcagaagctgcgcgccctcatcgccgagaagagctgcgcccctctcatgctccgcctcgcgtaagccgtcgccctcgcactcgctcgcttctaggttttgtgtagaggcggatttccgtagactgatccggtttttgtggagacggatcgcgcaggtggcactcggcggggacgttcgacgtgtcgtcgaagaccgggggcccgttcgggacgatgaagaccccggcggagctgtcgcacgccgccaacgcggggctggacatcgcggtgcggatgctcgagcccatcaaggaggagatacccaccatctcctacgccgatttctaccaggttcgccgcgagccccggatagatctggatggacggccccgatctggtcaaacggacgtggttctgatgttttttccccgttgtttttgcgcagcttgccggagttgtggccgtcgaggtgtccggtggacctgccgtccccttccacccaggaagggaggtgagatttcctattacctttcaactcttggggtttttctcagggttctagttaggccttgatatggcagtggtaaactggatggcgtgtggatttgtttgtgatgccacgagattagtcgtaatttgttagtgctccattgatgtgtgtgcttttcatttgtggggactgggggttggtggttcctagagagtagacaaagtgatcaccactaatctaaggccagcagtataataatattttaacagtacggacttttctatattatatacccggataataccgtcaaattgggtttgttcgtattcttgttggtgggtcggcgttacttgacatcctctagatctagaggtggttaaatatccgtgacctcatttacatttccgtaagaccttgtttatttaccataaacatgctaattgttattttttagttattgttggctcatgtgtttgtgtcagttgagtcacataggattcattttcatgcatgctgtatggtgtgaagtttgttatccattcatgcacaaggatattatttttaaggatgtcgccttatcctttttctttgtagtctggctattctcaaagtagtcactgatttaccatggttgtttgcggcatttccctgttcagggttttgatagttactgatctttcattttatcatttttattttttactcaggatagtagttctcatcctaatcgatgtcatactctaaaccatgacaaataaacaattattttctgaattaatggaagaagccttggggacctggaagaagccttggggaccggggttgtttccgttctctatgcatgatatagtttgatctgttggattgtgccatgtaggatatttgttgtgctttgtgaatataaacttaagaaagcatccattatttacaagtctagttggttcagataaagtatattagggcctcccaacttattgcattgacataaaatgataattaggggttcaatctgaaggcagttggaaagcttaatgaaaaaacagtgggccgatatatatctatatgttatgttttaggaaactttagaaaggtgacttggtgcttgttttaggatataataactgttatggatatatctttttttataaatacataatgttcagtaattattatgcaccttcaacaatatcaccgatgatagtaatatatatcatatatgtaacattatacagtgcttaaaatttgcttgtgcatatctggctcatctttatatttatttctaaaatggagagatgattaagtgtttttttatgttccattttatattcattgtgttgttaattgagattgttttcatccttccttgaccttttgcaggacaaacctgcacccccacctgagggccgtcttcctgatgctaccaagggtatgttgccagtttacactactagtctcttccaggaactatagaaacattcaccactattgccataaaagaaacccttgcaaataagtcatcctttaaaatgtttttgtttctgcaatagttgaggtgttatttctgtgtagcacatctaaaatgcttagttatggtagaagtttttcccactgcacacttgacaaatgattcctgtgttgttgtaggcatgttgtatttttctgcatgcttcatttctgatatgatcctgctttgaccttgttctagtaaacctcatgtaaagcaccattgcttacactttgagcttgattgctttaccaggttctgaccacctaaggcaggtcttcggtgcgcagatgggcttgagtgatcaggacattgttgccctctctggcggtcacaccctggttagtttggctattaaccattgtatgcctctgcagtatgtaggagtacatgttctaatgtcggatttctgtgctttgttctgcagggaaggtgccacaaggaaagatctggttttgagggaccttggacaagaaaccctctgcagtttgacaactcttacttcacgtaagcgatgttacgagtggttgaattttgttcttatgctgaacaaagttggtaacaaaaatgttgaactcatctctccgcgacgattttaatagggagcttctgagtggtgacaaggagggccttcttcagcttcctagtgacaaagccctgctgagtgaccctgccttccgcccactcgtcgagaaatatgctgcagtatgtctcctatcaatcatcatcttgtgttccacgttttgcatcttgtgttcctcgttttgcagagatatgccagaacatgatactgattgtgttttttttccccaattctgctaggatgagaaggctttctttgaagactacaaggaggcccacctcaagctctccgaactggggtgagtaatggtttgtcaatccatttatcgtgttatctttcatgacaacaaggtcatggtagctaattgtactcttgtgatttcaggttcgctgatgcttaagaggtttctagtctactactgctagtacattgcccgtggtactcttgttttgcatctaggctagggccagtgtgaaccagcagactaccactgtgagtcgcctctgtaataaaatttgttcggcctatggccatcctcagtacgttgtcaatgtctcggtgggctgttatgctcaactgcaatgctgcatccactgaaacctctttctagatgtgttggtatgaaatgcggtattgcgttcccgatttcccc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001056304.1 RefSeq:Os03g0285700]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

Os03g0170900

2014-06-10T11:27:14Z

Sean: /* Evolution */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.
It's the first sucrose transporter gene in rice, rice sucrose transport gene, named ''OsSUT1''. It might not play an important role in carbon metabolism of rice, but participate in the long distance transportation of assimilation product, and plays an important role in the process of sucrose by apoplast pathway.
The expression changes of ''OsSUT1'' gene adjusted salt stress response of the Taipei 309 rice varieties (Siahpoosh et al. 2012).

OsSUT1 didn’t directly involve in sucrose transport between epithelial cells in seed scutellum or between aleurone cells during the process of germination. However, in the companion cells and keratin vascular bundle, OsSUT1 may participate in the sucrose loading of phloems to transport to the developing buds and roots. OsSUT1 may also participate in regaining sucrose from the phloem tissue of taproots, coleoptiles and first and second leaves and other parts (Scofield et al., 2007).

Rice sucrose transporters OsSUT1, participate in transport of assimilation product over long distances. OsSUT1 function has been previously tested in the germination and early seedling growth. In this article, the author tested the role of OsSUT1 in assimilate during the whole transport over long distances (from flag blades to the base of the grouting seeds). GUS expression drived by OsSUT1 promoter and immune positioning with OsSUT1 shows that, in the process of grouting, GUS activity and OsSUT1 protein appeared in the mature phloem organized of all the nutrients involved in the assimilation product long-distance transportation way. In addition, OsSUT1 is detected expressing in flag foliage and leaf sheath before earing. GUS activity mainly appeared in the phloem companion cell cell, while its protein in the companion cell and molecular sieve tube. RT-PCR detection showed OsSUT1 transcriptions expressed in the toppest internode (internode 1). The location datas of OsSUT1 is concerned with the starch and sucrose content in these organizations and carbohydrates stored in the stems. In order to detect the connection between the cells, we proceeded the feeding dyeing experiments. The results showed that the symplast between blades of flag leaves, leaf sheath and internode 1 phloem have continuity with parenchyma cells around. Therefore, it’s believed that, during the process of sucrose transport, OsSUT1 may participate in recycling spilled sucrose (Scofield et al., 2007).

Destruction of rice sucrose transporters OsSUT1 damage the function of pollen but does not affect the mature of pollen. As is known to all, in all kinds of sucrose transport steps, sucrose transporters (SUTs) plays an important role in the process of sucrose by apoplast pathway. Due to the developing pollen is symplast separated withanther tissue, SUTs may play a role in the process of sucrose by apoplast pathway. In order to verify this possibility, we anlyze SUT gene family in pubertal pollen of rice by RT-PCR and laser micro-dissection. In five SUT genes, ''OsSUT1'' and ''OsSUT3'' expressed in pollen, while the expression of both have time specificity and are not identical. ''OsSUT1'' are expressed in pollen, determined by using ''OsSUT1'' promoter drive GUS expression. In order to further study ''OsSUT1'' function, we found insertion mutants.Tthe homozygous mutants (''SUT1'' - / -) can’t be obtained Heterozygous mutants (''SUT1'' + / -) have grain with normal grouting. However, the lose of ''OsSUT1'' does not affect the starch accumulation in the process of pollen development, suggesting that there may be other sucrose transporters involved in starch biosynthesis (Hirose et al., 2010).

Five sucrose transport gene of rice are expressed in xenopus oocyte and determined by the method of electrophysiology for their activities. It showed that OsSUT1 and OsSUT5 can generate sucrose-dependent electricity through the two electrode voltage clamp technique analysis. We tested the dynamic character of sucrose transport, substrate specificity, PH dependency and sucrose K0.5. OsSUT1 show the similar characteristics with ⅡSUT of all monocotyledon detected before: K0.5 is 7.50 mM, the optimal pH5.6. Compared with OsSUT1, substrate affinity of OsSUT5 is slightly higher (K0.5 is 2.32 mM, the optimal pH5.6). When compared with all Ⅱ SUTs before, substrate specificity of OsSUT5 is a little poorer, pH dependence lower. Meanwhile, the authour detected the regulation of reduced glutathione GSH and oxidized glutathione GSSG for each OsSUTs and ZmSUT1 in corn and HvSUT1 in barley, and found GSH and GSSG had no significant effect on these SUTs activity in xenopus oocyte. In conclusion, transport active differences between OsSUT1 and OsSUT5 has certain scope, which may be adapted to their functions in plants (Sun et al., 2010)

===Expression===
Please input expression information here.
''OsSUT1'' is expressed highly in the germination of the seed source and ears, lowly in roots (Aoki et al., 2003).

GUS expression drived by OsSUT1 promoter and immune positioning with OsSUT1 shows that, in the process of grouting, GUS activity and OsSUT1 protein appeared in the mature phloem organized of all the nutrients involved in the assimilation product long-distance transportation way. In addition, OsSUT1 is detected expressing in flag foliage and leaf sheath before earing. GUS activity mainly appeared in the phloem companion cell cell, while its protein in the companion cell and molecular sieve tube. （Scofield et al., 2007）

In five SUT genes, ''OsSUT1'' and ''OsSUT3'' expressed in pollen, while the expression of both have time specificity and are not identical. ''OsSUT1'' are expressed in pollen, determined by using ''OsSUT1'' promoter drive GUS expression. （Hirose et al., 2010）

===Evolution===
Please input evolution information here.
Using ''OsSUT1'' and ''HvSUT2'' blast rice genome and get four SUTs, ''OsSUT2'', ''OsSUT3'', ''OsSUT4'' and ''OsSUT5''. These four genes and ''OsSUT1'' constitutes the sucrose transporters of rice gene families. The structure prediction of proteins translated from five SUT genes show that all have 12 transmembrane helical and a domain structure highly conserved in all known SUTs, suggesting the four ''SUTs'' coding functional sucrose transporters.

==Labs working on this gene==
Please input related labs here.

1. National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602 Japan.

2. Hokuriku National Agricultural Experiment Station, 1-2-1 Inada, Joetsu, Niigata, 943-0193 Japan.

3. Hokuriku Research Center, National Agricultural Research Center, National Agriculture and Food Research Organization, Joetsu, Niigata 943-0193, Japan.

4. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou, Jiangsu, China.

5. Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.

6. CSIRO Plant Industry, Canberra, ACT 2601, Australia.

7. Department of Rice Research, National Agricultural Research Center, Joetsu, Niigata, 943-0193 Japan.

8. Shahid Chamran University, College of Agriculture, Department of Agronomy and Plant Breeding, P.O. Box 61357-83151, Ahwaz, Iran.

9. University of California, San Diego, Division of Biological Sciences, Section of Cell and Developmental Biology, 9500 Gilman Drive #0116, Muir Biology Building, La Jolla, CA 92093, USA.

==References==
Please input cited references here.
1. Mohammad R. Siahpoosh;Diego H. Sanchez;Armin Schlereth;Graham N. Scofield;Robert T. Furbank;Joost T. van Dongen;Joachim Kopka
Modification of OsSUT1 gene expression modulates the salt response of rice Oryza sativa cv. Taipei 309
Plant Science, 2012, 182: 101-111
2. Tatsuro Hirose;Zujian Zhang;Akio Miyao;Hirohiko Hirochika;Ryu Ohsugi;Tomio Terao
Disruption of a gene for rice sucrose transporter, OsSUT1, impairs pollen function but pollen maturation is unaffected
Journal of Experimental Botany, 2010, 61(13): 3639-3646
3. Ye Sun;Anke Reinders;Kathryn R. LaFleur;Toko Mori;John M. Ward
Transport Activity of Rice Sucrose Transporters OsSUT1 and OsSUT5
Plant and Cell Physiology, 2010, 51(1): 114-122
4. Graham N. Scofield;Naohiro Aoki;Tatsuro Hirose;Makoto Takano;Colin L. D. Jenkins;Robert T. Furbank
The role of the sucrose transporter, OsSUT1, in germination and early seedling growth and development of rice plants
Journal of Experimental Botany, 2007, 58(3): 483-495
5. Graham N. Scofield;Tatsuro Hirose;Naohiro Aoki;Robert T. Furbank
Involvement of the sucrose transporter, OsSUT1, in the long-distance pathway for assimilate transport in rice
Journal of Experimental Botany, 2007, 58(12): 3155-3169
6. Naohiro Aoki;Tatsuro Hirose;Graham N. Scofield;Paul R. Whitfeld;Robert T. Furbank
The Sucrose Transporter Gene Family in Rice
Plant and Cell Physiology, 2003, 44(3): 223-232
7. Graham N. Scofield;Tatsuro Hirose;Judy A. Gaudron;Robert T. Furbank;Narayana M. Upadhyaya;Ryu Ohsugi
Antisense suppression of the rice transporter gene, OsSUT1, leads to impaired grain filling and germination but does not affect photosynthesis
Functional Plant Biology, 2002, 29(7): 815-826
8. Ken Ishimaru;Tatsurou Hirose;Naohiro Aoki;Sakiko Takahashi;Kiyomi Ono;Shinichi Yamamoto;Jiangzhong Wu;Shoko Saji;Tomoya Baba;Masashi Ugaki;Takashi Matsumoto;Ryu Ohsugi
Antisense Expression of a Rice Sucrose Transporter OsSUT1 in Rice (Oryza sativa L.)
Plant and Cell Physiology, 2001, 42(10): 1181-1185

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0170900|
Description = Sucrose transporter|
Version = NM_001055646.1 GI:115451020 GeneID:4331775|
Length = 6326 bp|
Definition = Oryza sativa Japonica Group Os03g0170900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:3776143..3782468|
CDS = 3776388..3776561,3776678..3776721,3776805..3776868,3776971..3777204,3777314..3777457 ,3777576..3777770,3777996..3778071,3778238..3778337,3778504..3778537 ,3778625..3778709,3778775..3778839,3778929..3779027,3779359..3779424 ,3782034..3782270|
GCID = <gbrowseImage1>
name=NC_008396:3776143..3782468
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:3776143..3782468
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctcgcggcagcggggccggaggaggcggcggcggtggtggcggggggctggagctgtcggtcggggtcggcggcggcggcggcgcgcgtggtgggggaggcggtgaggcggcggcggcggtggagacggcggcgccgatcagcctcgggaggctcatcctctccgggatggtcgccggcggcgtgcagtacgggtgggcgctccagctctccctcctcaccccctacgtccagacactcggactgtcgcatgctcttacttcattcatgtggctctgtggtcctattgctggcatggtggttcaaccatgcgtgggtctatacagtgataggtgcacctcaaaatggggaagacgaaggccatatattcttacaggatgtgtgttgatatgcttggctgtcgtagttattggattttcagctgacattggatacgccatgggtgatacaaaggaagactgcagtgtctaccatggatcacggtggcatgctgctattgtatatgtcctgggattctggcttcttgatttttccaacaacactgtgcagggtccagcacgtgctctaatggctgatttatcaggacgtcatggacctggcacagctaactccatcttctgttcttggatggcaatgggaaacatcctaggatactcctctggttccacaaacaattggcacaagtggtttccattccttaaaacaagagcttgctgtgaagcttgtgcaaatttgaaaggtgcatttctggtggctgtgattttcctgtccctgtgcttggttataactctgatatttgccaaggaagttccattcaaaggaaatgcagccctcccaacaaaatcaaacgagccagctgagcctgaaggcactggtccactagcagtgttgaaaggcttcaggaacttacctactgggatgccttctgtgcttattgtgactggccttacctggttgtcttggttcccattcatcctctacgacactgactggatgggccgtgagatctaccacggtgacccaaagggaactgatcctcagatcgaggctttcaaccagggtgtcagggcaggtgcatttggcctgctactgaattcgattgtgctaggattcagctcattcttgattgaaccaatgtgccggaaagtcgggccaagggtggtgtgggtgacgagcaatttcctcgtctgcatcgccatggctgcaactgcactgatcagcttctggtcactcaaggacttccatggaactgttcagaaagccatcactgcagacaagagcatcaaggctgtctgcctcgtcctctttgcgtttcttggagtccctctcgccgttctgtacagtgttcccttcgcagtgacggcgcaattagcggccactagagggggtggccaagggctctgcaccggcgtcctcaacatctccatcgtcatccctcaggtggtcatcgcgctgggagccgggccgtgggacgagctgttcgggaaggggaacatcccggcgttcggcctcgcctcgggattcgcgctcatcggcggcgtcgccggaatattcctgctgcccaagatctccaagcgccagttccggtcggtcagcatgggcggcggtcactga</cdnaseq>|
AA = <aaseq>MARGSGAGGGGGGGGGGLELSVGVGGGGGARGGGGGEAAAAVET AAPISLGRLILSGMVAGGVQYGWALQLSLLTPYVQTLGLSHALTSFMWLCGPIAGMVV QPCVGLYSDRCTSKWGRRRPYILTGCVLICLAVVVIGFSADIGYAMGDTKEDCSVYHG SRWHAAIVYVLGFWLLDFSNNTVQGPARALMADLSGRHGPGTANSIFCSWMAMGNILG YSSGSTNNWHKWFPFLKTRACCEACANLKGAFLVAVIFLSLCLVITLIFAKEVPFKGN AALPTKSNEPAEPEGTGPLAVLKGFRNLPTGMPSVLIVTGLTWLSWFPFILYDTDWMG REIYHGDPKGTDPQIEAFNQGVRAGAFGLLLNSIVLGFSSFLIEPMCRKVGPRVVWVT SNFLVCIAMAATALISFWSLKDFHGTVQKAITADKSIKAVCLVLFAFLGVPLAVLYSV PFAVTAQLAATRGGGQGLCTGVLNISIVIPQVVIALGAGPWDELFGKGNIPAFGLASG FALIGGVAGIFLLPKISKRQFRSVSMGGGH</aaseq>|
DNA = <dnaseqindica>5908..6081#5748..5791#5601..5664#5265..5498#5012..5155#4699..4893#4398..4473#4132..4231#3932..3965#3760..3844#3630..3694#3442..3540#3045..3110#199..435#aaacacaaacccaccacctcctcctcctcctcccatccagcacgcgcctcctctctcgcgcggctttccatttccatctccccctcctcctcctacgtctccgccgctcctcacttcctccactcgatttcctttcttggcctctcctcctctgacacaggggtgtgcaggtttgtgtttgtgcgtggcgcgtccgccatggctcgcggcagcggggccggaggaggcggcggcggtggtggcggggggctggagctgtcggtcggggtcggcggcggcggcggcgcgcgtggtgggggaggcggtgaggcggcggcggcggtggagacggcggcgccgatcagcctcgggaggctcatcctctccgggatggtcgccggcggcgtgcagtacgggtgggcgctccagctctccctcctcaccccctacgtccaggtatatatatatatatactgtactcctgtgtattttgcacactctcatgcatgcatctgaaactctgaataatcctgcaaaaaatatatacaagtatctcacgtgtggatttgattttgttcgcgagtttttgctcgtgctagctgttctaatctgcaaggtttcatggtagtagattatatatgtcctggttacaatcttttcaaggttataatcttgtaatttttttctgctttgtcaatatttacttcgcactgtctggtgtgagtcgttaaaaaaatatatatcttgaaagattggggaatttttcttcatgtgtggggagagatttatttttttttgtttgaacggtagatttgactagtgtctaaaagtaccgtaataaattcaatctgcatgccttttgtaattttttttgctcatgtgtttatatacatgtggccaaatatgcatttgatgcactagctatcatttttgtcattttgtagttattccttttcctttcctttttttttcctttttctttttgttgtcactagaggattcatatcctctcgattcctctatgaaacgtcggcgttttctgcctcttttctgaagaatctgtatacctatataagaatcaaaatcttttataagttctttttgtgcactttggatcaatcaggctagccagcatgacacttagcatcagtaccggtctactgtaccgagattcactttacgcttgacacgtttctcaactccttgtttgattccattttacctcaattagactgtttgattccgttttatctcagatatgacactcctttttttaaaattccattttacctcagatgctgtacagtgccataaaaaaatatacatctgctccaactcaccttatccttcttgtaataaccacgtattaggttaatagctactaatagctagagatgtaaacaaaaataagcagacaagggcaaaagtgagcggtaataatggagaattaatctgtcgttcctccgcgcgcagttaaaggtaggcagatgacgggtccctcccctctattccatactaaccgcaagatgaaactaatattcttactgctagatatataaatcatccttcatttagcttcatctccctccttaagatgtgttcttttcttccatttcctaacttctactcccttcgtttgacgtacacatatttcctaaactgccaaacgatgtgtttctactaaaaatttatatggtttaaaaaacatattaatatatttttttttgtctttttagctaatatttaattaattatgtgctaaactaccgctcaattttgtgtaccgggagaaagggttctcaatcattacaaaagaacacagcctagtctccatgaaaaacatcacttttattttaagacagagcaagtggagcgattttataactttactatagtacaaaattaatggtcagaaatttaaaattttgaccgaattttatcgtaagcatcaaatatttattaccggagagatagagtattggttatttgcctctctgtcgtcaaatattctaaacaaagacgatcttcttagtggttaaacttaaatctcagtccttccaggcgtttatttgaaggatttatagcggagataaacagctccttttttttcggaaatacgtaaggagggcacttttgtattaaggggaataaaaagggtctgtacagggaaaaccaaaaaacagcaggggacaggaataaacagctcctcacttcctctttgtatcagtccatactaattaacaaagtgttccaaaatgttctgtactttttctgtagaacattatatgcagttcatcacccctgatggtccatgcatcaggtctagctacctactgttgtattgaagtatttctggcgaagtttatccttaccttagccttctcgagccaagtgccataaagatggttgctgtagaggcaactccaacccactttaaccgcgcacatgaaaaataaaggggtaaaacaaggaaattctaggtcagatatatatgtcctgtggactgtggcacagttgcaacagagcaattgcataggcaaatgagggcagatcatcatgtacccatagcctgttggtgctatgttagcatccaagcattgccattttaactattaagattcttcttcttatatttgacaagtgaaataaatttagtgactgaacatatatagaacgatgaacagtctttgtatgtttattcatgccatgggggaaagagaagtgtgatctgaagttattggatagatagctgttagttgtagcatgcaaggcaatgcatcctggaaccaacagaccaacagagctggggatatttcatctgctaaatttctgggaaaatttattgtgtggtaatcaatatgtgttcactctttgcaactggtacacgctctctgatgtttctgaatatcttccacagtgatgcagatcttgttgacacagcaaactcaacaaacatagtcatttttttaaggaatgaagcatgatcatctattgtcagagtagattacagtcaaagtttttttataagccaaaacaactgattttctaacctctgttatttacagacactcggactgtcgcatgctcttacttcattcatgtggctctgtggtcctattgctggcatggtggtaagtaacaataccaaattatctgctctatcgatatatacgagacaaagcttttgccctcttttaaaaaaaataaatcaaattaattcatgttatggaatctgtactttctgcaatgtaaattcaagttactttagtgtcttcactacaacaaaaacttgggtatcctgaactcctgtatctatcagagtttgaagctgcattttctgtagcttctgatatgtgtaatgtagatcatactgtcataattcataagctttcataaaaaatgtttgtgtacaagtcatattttttttaattcttgatccattatcatctcccaatcctgcaggttcaaccatgcgtgggtctatacagtgataggtgcacctcaaaatggggaagacgaaggccatatattcttacaggatgtgtgttgatatgcttggctgtaagaaccactgaaccttggaactcttattccttaagttgaagtccccaaattttctctcatattgacattgcactttatgtatgtaggtcgtagttattggattttcagctgacattggatacgccatgggtgatacaaaggaagactgcaggtaactgaacattttgcgtatcataattagcagataacagttatctgatacaccatgtaatgcagtgtctaccatggatcacggtggcatgctgctattgtatatgtcctgggattctggcttcttgatttttccaacaacactgtgcaggttagtgcctactatataataatcatgttaaagaaatttgtacaaagaatcataacaaaaatcttaattttgcaatgcaatttctagggtccagcacgtgctctaatggctgatttatcaggtaatttttcatagccttgtctttcagagttaactaccaaatttttattggtttccacagttcactaacacttgattgattccagaaaaaaaaaagttgcccttgtgttctgggcttctggtatcagtgggattttggatttgatctcctaattttcgttctgtaggacgtcatggacctggcacagctaactccatcttctgttcttggatggcaatgggaaacatcctaggatactcctctggttccacaaacaattggcacaagtaagttactcttatgtatcagtttcatacagattttctgtttagcacagtctattcagttgcattcttttctgaaagaaggcaagtgaaattcatattacattagtagagaaggtgaaatttgtttacaagtattgagttgcattctccctaattaattgcgcaggtggtttccattccttaaaacaagagcttgctgtgaagcttgtgcaaatttgaaaggtgcatttctggtggctgtggtaagttctgttattacctcataaactgcctgctgataatactttaacaatgtgctaatattagtctttgtaataagatagtactatactgaaaatattttagcgagtatgagtaatttaacttacatattgtattgctgttcctcttttttcaaccctgtcatattggttgctttttttcacagcctaacatgctcttgtttggtcattttcccctgttttcagattttcctgtccctgtgcttggttataactctgatatttgccaaggaagttccattcaaaggaaatgcagccctcccaacaaaatcaaacgagccagctgagcctgaaggcactggtccactagcagtgttgaaaggcttcaggaacttacctactgggatgccttctgtgcttattgtgactggccttacctgggtatgcaatatacatcagaaaaagaacttttcagattattgaaaaaattgctactagatcatatcttagttatatcttatagtaccttaccttttctatgatatgattgtccttgtagttgtcttggttcccattcatcctctacgacactgactggatgggccgtgagatctaccacggtgacccaaagggaactgatcctcagatcgaggctttcaaccagggtgtcagggcaggtgcatttggcctgctactgaattcggtaagatctgattggtttctgaattctgattgccacaactactgtcaaatctttaacactcaagccatgttctttggcagcaactaaccatgggcttctgatcgtgcagattgtgctaggattcagctcattcttgattgaaccaatgtgccggaaagtcgggccaagggtggtgtgggtgacgagcaatttcctcgtctgcatcgccatggctgcaactgcactgatcagcttctggtcactcaaggacttccatggaactgttcagaaagccatcactgcagacaagagcatcaaggctgtctgcctcgtcctctttgcgtttcttggagtccctctcgccgtaagaatcactgacacttcaacttaaacacattgctaaaaaaaattatttcctcaagaatgccttatcctgatgaactgtctgaaaatttgcgcgatgcaggttctgtacagtgttcccttcgcagtgacggcgcaattagcggccactagagggggtggccaaggtatgaatcccaacattcttgcagcttttcttgacataattcggtgttgtttggatgctggtctaatccatggcgatctgcagggctctgcaccggcgtcctcaacatctccatcgtcatccctcaggttaattagctgccaaattctaatccgtttcccttgaaaccgaatcagatatacttcatggattattagaatttgaatgctcatggattcatggttgcgtggtgttcttccctcaggtggtcatcgcgctgggagccgggccgtgggacgagctgttcgggaaggggaacatcccggcgttcggcctcgcctcgggattcgcgctcatcggcggcgtcgccggaatattcctgctgcccaagatctccaagcgccagttccggtcggtcagcatgggcggcggtcactgaccgccccgctctcgccgccggctgcggcggcggcggcggccggagcagcgccgccggaagtggtgagctttgtagttctcgtttgcaatggatcaaaggagccccctctttttttttacatagggtaattaattaattaaggctgttaattaggaaggggtgaggtggccctgcagctggggcccacgctttgttgccacctcggtagaagagaataagcagatcatgcagataatatggaactg</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055646.1 RefSeq:Os03g0170900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

Os03g0170900

2014-06-10T11:26:08Z

Sean: /* Evolution */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.
It's the first sucrose transporter gene in rice, rice sucrose transport gene, named ''OsSUT1''. It might not play an important role in carbon metabolism of rice, but participate in the long distance transportation of assimilation product, and plays an important role in the process of sucrose by apoplast pathway.
The expression changes of ''OsSUT1'' gene adjusted salt stress response of the Taipei 309 rice varieties (Siahpoosh et al. 2012).

OsSUT1 didn’t directly involve in sucrose transport between epithelial cells in seed scutellum or between aleurone cells during the process of germination. However, in the companion cells and keratin vascular bundle, OsSUT1 may participate in the sucrose loading of phloems to transport to the developing buds and roots. OsSUT1 may also participate in regaining sucrose from the phloem tissue of taproots, coleoptiles and first and second leaves and other parts (Scofield et al., 2007).

Rice sucrose transporters OsSUT1, participate in transport of assimilation product over long distances. OsSUT1 function has been previously tested in the germination and early seedling growth. In this article, the author tested the role of OsSUT1 in assimilate during the whole transport over long distances (from flag blades to the base of the grouting seeds). GUS expression drived by OsSUT1 promoter and immune positioning with OsSUT1 shows that, in the process of grouting, GUS activity and OsSUT1 protein appeared in the mature phloem organized of all the nutrients involved in the assimilation product long-distance transportation way. In addition, OsSUT1 is detected expressing in flag foliage and leaf sheath before earing. GUS activity mainly appeared in the phloem companion cell cell, while its protein in the companion cell and molecular sieve tube. RT-PCR detection showed OsSUT1 transcriptions expressed in the toppest internode (internode 1). The location datas of OsSUT1 is concerned with the starch and sucrose content in these organizations and carbohydrates stored in the stems. In order to detect the connection between the cells, we proceeded the feeding dyeing experiments. The results showed that the symplast between blades of flag leaves, leaf sheath and internode 1 phloem have continuity with parenchyma cells around. Therefore, it’s believed that, during the process of sucrose transport, OsSUT1 may participate in recycling spilled sucrose (Scofield et al., 2007).

Destruction of rice sucrose transporters OsSUT1 damage the function of pollen but does not affect the mature of pollen. As is known to all, in all kinds of sucrose transport steps, sucrose transporters (SUTs) plays an important role in the process of sucrose by apoplast pathway. Due to the developing pollen is symplast separated withanther tissue, SUTs may play a role in the process of sucrose by apoplast pathway. In order to verify this possibility, we anlyze SUT gene family in pubertal pollen of rice by RT-PCR and laser micro-dissection. In five SUT genes, ''OsSUT1'' and ''OsSUT3'' expressed in pollen, while the expression of both have time specificity and are not identical. ''OsSUT1'' are expressed in pollen, determined by using ''OsSUT1'' promoter drive GUS expression. In order to further study ''OsSUT1'' function, we found insertion mutants.Tthe homozygous mutants (''SUT1'' - / -) can’t be obtained Heterozygous mutants (''SUT1'' + / -) have grain with normal grouting. However, the lose of ''OsSUT1'' does not affect the starch accumulation in the process of pollen development, suggesting that there may be other sucrose transporters involved in starch biosynthesis (Hirose et al., 2010).

Five sucrose transport gene of rice are expressed in xenopus oocyte and determined by the method of electrophysiology for their activities. It showed that OsSUT1 and OsSUT5 can generate sucrose-dependent electricity through the two electrode voltage clamp technique analysis. We tested the dynamic character of sucrose transport, substrate specificity, PH dependency and sucrose K0.5. OsSUT1 show the similar characteristics with ⅡSUT of all monocotyledon detected before: K0.5 is 7.50 mM, the optimal pH5.6. Compared with OsSUT1, substrate affinity of OsSUT5 is slightly higher (K0.5 is 2.32 mM, the optimal pH5.6). When compared with all Ⅱ SUTs before, substrate specificity of OsSUT5 is a little poorer, pH dependence lower. Meanwhile, the authour detected the regulation of reduced glutathione GSH and oxidized glutathione GSSG for each OsSUTs and ZmSUT1 in corn and HvSUT1 in barley, and found GSH and GSSG had no significant effect on these SUTs activity in xenopus oocyte. In conclusion, transport active differences between OsSUT1 and OsSUT5 has certain scope, which may be adapted to their functions in plants (Sun et al., 2010)

===Expression===
Please input expression information here.
''OsSUT1'' is expressed highly in the germination of the seed source and ears, lowly in roots (Aoki et al., 2003).

GUS expression drived by OsSUT1 promoter and immune positioning with OsSUT1 shows that, in the process of grouting, GUS activity and OsSUT1 protein appeared in the mature phloem organized of all the nutrients involved in the assimilation product long-distance transportation way. In addition, OsSUT1 is detected expressing in flag foliage and leaf sheath before earing. GUS activity mainly appeared in the phloem companion cell cell, while its protein in the companion cell and molecular sieve tube. （Scofield et al., 2007）

In five SUT genes, ''OsSUT1'' and ''OsSUT3'' expressed in pollen, while the expression of both have time specificity and are not identical. ''OsSUT1'' are expressed in pollen, determined by using ''OsSUT1'' promoter drive GUS expression. （Hirose et al., 2010）

===Evolution===
Please input evolution information here.
Using ''OsSUT1'' and ''HvSUT2'' blast rice genome and get four SUTs, ''OsSUT2'', ''OsSUT3'', ''OsSUT4'' and ''OsSUT5''. These four genes and ''OsSUT1'' constitutes the sucrose transporters of rice gene families. The structure prediction of proteins translated from five SUT genes show that all have 12 transmembrane helical and a domain structure highly conserved in all known SUTs, suggesting the four ''SUTs'' coding functional sucrose transporters.

[[File:fig1.jpg|thumb|150px|An un-rooted dendrogram of plant SUTs, based on deduced amino acid sequences. The CLUSTALW program was used to show the relationship between the members of the OsSUT gene family (bold) and other plant SUTs.]]

==Labs working on this gene==
Please input related labs here.

1. National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602 Japan.

2. Hokuriku National Agricultural Experiment Station, 1-2-1 Inada, Joetsu, Niigata, 943-0193 Japan.

3. Hokuriku Research Center, National Agricultural Research Center, National Agriculture and Food Research Organization, Joetsu, Niigata 943-0193, Japan.

4. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou, Jiangsu, China.

5. Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.

6. CSIRO Plant Industry, Canberra, ACT 2601, Australia.

7. Department of Rice Research, National Agricultural Research Center, Joetsu, Niigata, 943-0193 Japan.

8. Shahid Chamran University, College of Agriculture, Department of Agronomy and Plant Breeding, P.O. Box 61357-83151, Ahwaz, Iran.

9. University of California, San Diego, Division of Biological Sciences, Section of Cell and Developmental Biology, 9500 Gilman Drive #0116, Muir Biology Building, La Jolla, CA 92093, USA.

==References==
Please input cited references here.
1. Mohammad R. Siahpoosh;Diego H. Sanchez;Armin Schlereth;Graham N. Scofield;Robert T. Furbank;Joost T. van Dongen;Joachim Kopka
Modification of OsSUT1 gene expression modulates the salt response of rice Oryza sativa cv. Taipei 309
Plant Science, 2012, 182: 101-111
2. Tatsuro Hirose;Zujian Zhang;Akio Miyao;Hirohiko Hirochika;Ryu Ohsugi;Tomio Terao
Disruption of a gene for rice sucrose transporter, OsSUT1, impairs pollen function but pollen maturation is unaffected
Journal of Experimental Botany, 2010, 61(13): 3639-3646
3. Ye Sun;Anke Reinders;Kathryn R. LaFleur;Toko Mori;John M. Ward
Transport Activity of Rice Sucrose Transporters OsSUT1 and OsSUT5
Plant and Cell Physiology, 2010, 51(1): 114-122
4. Graham N. Scofield;Naohiro Aoki;Tatsuro Hirose;Makoto Takano;Colin L. D. Jenkins;Robert T. Furbank
The role of the sucrose transporter, OsSUT1, in germination and early seedling growth and development of rice plants
Journal of Experimental Botany, 2007, 58(3): 483-495
5. Graham N. Scofield;Tatsuro Hirose;Naohiro Aoki;Robert T. Furbank
Involvement of the sucrose transporter, OsSUT1, in the long-distance pathway for assimilate transport in rice
Journal of Experimental Botany, 2007, 58(12): 3155-3169
6. Naohiro Aoki;Tatsuro Hirose;Graham N. Scofield;Paul R. Whitfeld;Robert T. Furbank
The Sucrose Transporter Gene Family in Rice
Plant and Cell Physiology, 2003, 44(3): 223-232
7. Graham N. Scofield;Tatsuro Hirose;Judy A. Gaudron;Robert T. Furbank;Narayana M. Upadhyaya;Ryu Ohsugi
Antisense suppression of the rice transporter gene, OsSUT1, leads to impaired grain filling and germination but does not affect photosynthesis
Functional Plant Biology, 2002, 29(7): 815-826
8. Ken Ishimaru;Tatsurou Hirose;Naohiro Aoki;Sakiko Takahashi;Kiyomi Ono;Shinichi Yamamoto;Jiangzhong Wu;Shoko Saji;Tomoya Baba;Masashi Ugaki;Takashi Matsumoto;Ryu Ohsugi
Antisense Expression of a Rice Sucrose Transporter OsSUT1 in Rice (Oryza sativa L.)
Plant and Cell Physiology, 2001, 42(10): 1181-1185

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0170900|
Description = Sucrose transporter|
Version = NM_001055646.1 GI:115451020 GeneID:4331775|
Length = 6326 bp|
Definition = Oryza sativa Japonica Group Os03g0170900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:3776143..3782468|
CDS = 3776388..3776561,3776678..3776721,3776805..3776868,3776971..3777204,3777314..3777457 ,3777576..3777770,3777996..3778071,3778238..3778337,3778504..3778537 ,3778625..3778709,3778775..3778839,3778929..3779027,3779359..3779424 ,3782034..3782270|
GCID = <gbrowseImage1>
name=NC_008396:3776143..3782468
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:3776143..3782468
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctcgcggcagcggggccggaggaggcggcggcggtggtggcggggggctggagctgtcggtcggggtcggcggcggcggcggcgcgcgtggtgggggaggcggtgaggcggcggcggcggtggagacggcggcgccgatcagcctcgggaggctcatcctctccgggatggtcgccggcggcgtgcagtacgggtgggcgctccagctctccctcctcaccccctacgtccagacactcggactgtcgcatgctcttacttcattcatgtggctctgtggtcctattgctggcatggtggttcaaccatgcgtgggtctatacagtgataggtgcacctcaaaatggggaagacgaaggccatatattcttacaggatgtgtgttgatatgcttggctgtcgtagttattggattttcagctgacattggatacgccatgggtgatacaaaggaagactgcagtgtctaccatggatcacggtggcatgctgctattgtatatgtcctgggattctggcttcttgatttttccaacaacactgtgcagggtccagcacgtgctctaatggctgatttatcaggacgtcatggacctggcacagctaactccatcttctgttcttggatggcaatgggaaacatcctaggatactcctctggttccacaaacaattggcacaagtggtttccattccttaaaacaagagcttgctgtgaagcttgtgcaaatttgaaaggtgcatttctggtggctgtgattttcctgtccctgtgcttggttataactctgatatttgccaaggaagttccattcaaaggaaatgcagccctcccaacaaaatcaaacgagccagctgagcctgaaggcactggtccactagcagtgttgaaaggcttcaggaacttacctactgggatgccttctgtgcttattgtgactggccttacctggttgtcttggttcccattcatcctctacgacactgactggatgggccgtgagatctaccacggtgacccaaagggaactgatcctcagatcgaggctttcaaccagggtgtcagggcaggtgcatttggcctgctactgaattcgattgtgctaggattcagctcattcttgattgaaccaatgtgccggaaagtcgggccaagggtggtgtgggtgacgagcaatttcctcgtctgcatcgccatggctgcaactgcactgatcagcttctggtcactcaaggacttccatggaactgttcagaaagccatcactgcagacaagagcatcaaggctgtctgcctcgtcctctttgcgtttcttggagtccctctcgccgttctgtacagtgttcccttcgcagtgacggcgcaattagcggccactagagggggtggccaagggctctgcaccggcgtcctcaacatctccatcgtcatccctcaggtggtcatcgcgctgggagccgggccgtgggacgagctgttcgggaaggggaacatcccggcgttcggcctcgcctcgggattcgcgctcatcggcggcgtcgccggaatattcctgctgcccaagatctccaagcgccagttccggtcggtcagcatgggcggcggtcactga</cdnaseq>|
AA = <aaseq>MARGSGAGGGGGGGGGGLELSVGVGGGGGARGGGGGEAAAAVET AAPISLGRLILSGMVAGGVQYGWALQLSLLTPYVQTLGLSHALTSFMWLCGPIAGMVV QPCVGLYSDRCTSKWGRRRPYILTGCVLICLAVVVIGFSADIGYAMGDTKEDCSVYHG SRWHAAIVYVLGFWLLDFSNNTVQGPARALMADLSGRHGPGTANSIFCSWMAMGNILG YSSGSTNNWHKWFPFLKTRACCEACANLKGAFLVAVIFLSLCLVITLIFAKEVPFKGN AALPTKSNEPAEPEGTGPLAVLKGFRNLPTGMPSVLIVTGLTWLSWFPFILYDTDWMG REIYHGDPKGTDPQIEAFNQGVRAGAFGLLLNSIVLGFSSFLIEPMCRKVGPRVVWVT SNFLVCIAMAATALISFWSLKDFHGTVQKAITADKSIKAVCLVLFAFLGVPLAVLYSV PFAVTAQLAATRGGGQGLCTGVLNISIVIPQVVIALGAGPWDELFGKGNIPAFGLASG FALIGGVAGIFLLPKISKRQFRSVSMGGGH</aaseq>|
DNA = <dnaseqindica>5908..6081#5748..5791#5601..5664#5265..5498#5012..5155#4699..4893#4398..4473#4132..4231#3932..3965#3760..3844#3630..3694#3442..3540#3045..3110#199..435#aaacacaaacccaccacctcctcctcctcctcccatccagcacgcgcctcctctctcgcgcggctttccatttccatctccccctcctcctcctacgtctccgccgctcctcacttcctccactcgatttcctttcttggcctctcctcctctgacacaggggtgtgcaggtttgtgtttgtgcgtggcgcgtccgccatggctcgcggcagcggggccggaggaggcggcggcggtggtggcggggggctggagctgtcggtcggggtcggcggcggcggcggcgcgcgtggtgggggaggcggtgaggcggcggcggcggtggagacggcggcgccgatcagcctcgggaggctcatcctctccgggatggtcgccggcggcgtgcagtacgggtgggcgctccagctctccctcctcaccccctacgtccaggtatatatatatatatactgtactcctgtgtattttgcacactctcatgcatgcatctgaaactctgaataatcctgcaaaaaatatatacaagtatctcacgtgtggatttgattttgttcgcgagtttttgctcgtgctagctgttctaatctgcaaggtttcatggtagtagattatatatgtcctggttacaatcttttcaaggttataatcttgtaatttttttctgctttgtcaatatttacttcgcactgtctggtgtgagtcgttaaaaaaatatatatcttgaaagattggggaatttttcttcatgtgtggggagagatttatttttttttgtttgaacggtagatttgactagtgtctaaaagtaccgtaataaattcaatctgcatgccttttgtaattttttttgctcatgtgtttatatacatgtggccaaatatgcatttgatgcactagctatcatttttgtcattttgtagttattccttttcctttcctttttttttcctttttctttttgttgtcactagaggattcatatcctctcgattcctctatgaaacgtcggcgttttctgcctcttttctgaagaatctgtatacctatataagaatcaaaatcttttataagttctttttgtgcactttggatcaatcaggctagccagcatgacacttagcatcagtaccggtctactgtaccgagattcactttacgcttgacacgtttctcaactccttgtttgattccattttacctcaattagactgtttgattccgttttatctcagatatgacactcctttttttaaaattccattttacctcagatgctgtacagtgccataaaaaaatatacatctgctccaactcaccttatccttcttgtaataaccacgtattaggttaatagctactaatagctagagatgtaaacaaaaataagcagacaagggcaaaagtgagcggtaataatggagaattaatctgtcgttcctccgcgcgcagttaaaggtaggcagatgacgggtccctcccctctattccatactaaccgcaagatgaaactaatattcttactgctagatatataaatcatccttcatttagcttcatctccctccttaagatgtgttcttttcttccatttcctaacttctactcccttcgtttgacgtacacatatttcctaaactgccaaacgatgtgtttctactaaaaatttatatggtttaaaaaacatattaatatatttttttttgtctttttagctaatatttaattaattatgtgctaaactaccgctcaattttgtgtaccgggagaaagggttctcaatcattacaaaagaacacagcctagtctccatgaaaaacatcacttttattttaagacagagcaagtggagcgattttataactttactatagtacaaaattaatggtcagaaatttaaaattttgaccgaattttatcgtaagcatcaaatatttattaccggagagatagagtattggttatttgcctctctgtcgtcaaatattctaaacaaagacgatcttcttagtggttaaacttaaatctcagtccttccaggcgtttatttgaaggatttatagcggagataaacagctccttttttttcggaaatacgtaaggagggcacttttgtattaaggggaataaaaagggtctgtacagggaaaaccaaaaaacagcaggggacaggaataaacagctcctcacttcctctttgtatcagtccatactaattaacaaagtgttccaaaatgttctgtactttttctgtagaacattatatgcagttcatcacccctgatggtccatgcatcaggtctagctacctactgttgtattgaagtatttctggcgaagtttatccttaccttagccttctcgagccaagtgccataaagatggttgctgtagaggcaactccaacccactttaaccgcgcacatgaaaaataaaggggtaaaacaaggaaattctaggtcagatatatatgtcctgtggactgtggcacagttgcaacagagcaattgcataggcaaatgagggcagatcatcatgtacccatagcctgttggtgctatgttagcatccaagcattgccattttaactattaagattcttcttcttatatttgacaagtgaaataaatttagtgactgaacatatatagaacgatgaacagtctttgtatgtttattcatgccatgggggaaagagaagtgtgatctgaagttattggatagatagctgttagttgtagcatgcaaggcaatgcatcctggaaccaacagaccaacagagctggggatatttcatctgctaaatttctgggaaaatttattgtgtggtaatcaatatgtgttcactctttgcaactggtacacgctctctgatgtttctgaatatcttccacagtgatgcagatcttgttgacacagcaaactcaacaaacatagtcatttttttaaggaatgaagcatgatcatctattgtcagagtagattacagtcaaagtttttttataagccaaaacaactgattttctaacctctgttatttacagacactcggactgtcgcatgctcttacttcattcatgtggctctgtggtcctattgctggcatggtggtaagtaacaataccaaattatctgctctatcgatatatacgagacaaagcttttgccctcttttaaaaaaaataaatcaaattaattcatgttatggaatctgtactttctgcaatgtaaattcaagttactttagtgtcttcactacaacaaaaacttgggtatcctgaactcctgtatctatcagagtttgaagctgcattttctgtagcttctgatatgtgtaatgtagatcatactgtcataattcataagctttcataaaaaatgtttgtgtacaagtcatattttttttaattcttgatccattatcatctcccaatcctgcaggttcaaccatgcgtgggtctatacagtgataggtgcacctcaaaatggggaagacgaaggccatatattcttacaggatgtgtgttgatatgcttggctgtaagaaccactgaaccttggaactcttattccttaagttgaagtccccaaattttctctcatattgacattgcactttatgtatgtaggtcgtagttattggattttcagctgacattggatacgccatgggtgatacaaaggaagactgcaggtaactgaacattttgcgtatcataattagcagataacagttatctgatacaccatgtaatgcagtgtctaccatggatcacggtggcatgctgctattgtatatgtcctgggattctggcttcttgatttttccaacaacactgtgcaggttagtgcctactatataataatcatgttaaagaaatttgtacaaagaatcataacaaaaatcttaattttgcaatgcaatttctagggtccagcacgtgctctaatggctgatttatcaggtaatttttcatagccttgtctttcagagttaactaccaaatttttattggtttccacagttcactaacacttgattgattccagaaaaaaaaaagttgcccttgtgttctgggcttctggtatcagtgggattttggatttgatctcctaattttcgttctgtaggacgtcatggacctggcacagctaactccatcttctgttcttggatggcaatgggaaacatcctaggatactcctctggttccacaaacaattggcacaagtaagttactcttatgtatcagtttcatacagattttctgtttagcacagtctattcagttgcattcttttctgaaagaaggcaagtgaaattcatattacattagtagagaaggtgaaatttgtttacaagtattgagttgcattctccctaattaattgcgcaggtggtttccattccttaaaacaagagcttgctgtgaagcttgtgcaaatttgaaaggtgcatttctggtggctgtggtaagttctgttattacctcataaactgcctgctgataatactttaacaatgtgctaatattagtctttgtaataagatagtactatactgaaaatattttagcgagtatgagtaatttaacttacatattgtattgctgttcctcttttttcaaccctgtcatattggttgctttttttcacagcctaacatgctcttgtttggtcattttcccctgttttcagattttcctgtccctgtgcttggttataactctgatatttgccaaggaagttccattcaaaggaaatgcagccctcccaacaaaatcaaacgagccagctgagcctgaaggcactggtccactagcagtgttgaaag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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055646.1 RefSeq:Os03g0170900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

File:Fig.1.jpg

2014-06-10T11:21:27Z

Sean: uploaded a new version of "File:Fig.1.jpg": An un-rooted dendrogram of plant SUTs, based on deduced amino acid sequences. The CLUSTALW program was used to show the relationship between the members of the OsSUT gene family (bold) and other pl

oul1 has increased bulliform cell number and size. A and B, Adaxial epidermal peels abutting the small veins of the wild type (wt; A) and oul1 (B). C to F, Cross sections of wild-type (C) and oul1 (D) mature leaf blades show significantly increased oul1 bulliform cell number (E) and area (F) between vascular bundle ridges. ab, Abaxial; ad, adaxial.
Red lines (C and D) show the bulliform cells. Data show means and SD values of biological replicates (n . 23) and statistical analysis by heteroscedastic t test indicating significant differences (** P , 0.01). Bars = 20 mm (A–D). G, Relative water content of the 10th leaf of 120- d-old greenhouse plants grown in the soil. oul1 had higher water content than the wild type. The data are means and SD (n . 5), with statistical analysis using the heteroscedastic t test showing significant differences (** P , 0.01).

Os03g0170900

2014-06-10T10:07:28Z

Sean: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.
It's the first sucrose transporter gene in rice, rice sucrose transport gene, named ''OsSUT1''. It might not play an important role in carbon metabolism of rice, but participate in the long distance transportation of assimilation product, and plays an important role in the process of sucrose by apoplast pathway.
The expression changes of ''OsSUT1'' gene adjusted salt stress response of the Taipei 309 rice varieties (Siahpoosh et al. 2012).

OsSUT1 didn’t directly involve in sucrose transport between epithelial cells in seed scutellum or between aleurone cells during the process of germination. However, in the companion cells and keratin vascular bundle, OsSUT1 may participate in the sucrose loading of phloems to transport to the developing buds and roots. OsSUT1 may also participate in regaining sucrose from the phloem tissue of taproots, coleoptiles and first and second leaves and other parts (Scofield et al., 2007).

Rice sucrose transporters OsSUT1, participate in transport of assimilation product over long distances. OsSUT1 function has been previously tested in the germination and early seedling growth. In this article, the author tested the role of OsSUT1 in assimilate during the whole transport over long distances (from flag blades to the base of the grouting seeds). GUS expression drived by OsSUT1 promoter and immune positioning with OsSUT1 shows that, in the process of grouting, GUS activity and OsSUT1 protein appeared in the mature phloem organized of all the nutrients involved in the assimilation product long-distance transportation way. In addition, OsSUT1 is detected expressing in flag foliage and leaf sheath before earing. GUS activity mainly appeared in the phloem companion cell cell, while its protein in the companion cell and molecular sieve tube. RT-PCR detection showed OsSUT1 transcriptions expressed in the toppest internode (internode 1). The location datas of OsSUT1 is concerned with the starch and sucrose content in these organizations and carbohydrates stored in the stems. In order to detect the connection between the cells, we proceeded the feeding dyeing experiments. The results showed that the symplast between blades of flag leaves, leaf sheath and internode 1 phloem have continuity with parenchyma cells around. Therefore, it’s believed that, during the process of sucrose transport, OsSUT1 may participate in recycling spilled sucrose (Scofield et al., 2007).

Destruction of rice sucrose transporters OsSUT1 damage the function of pollen but does not affect the mature of pollen. As is known to all, in all kinds of sucrose transport steps, sucrose transporters (SUTs) plays an important role in the process of sucrose by apoplast pathway. Due to the developing pollen is symplast separated withanther tissue, SUTs may play a role in the process of sucrose by apoplast pathway. In order to verify this possibility, we anlyze SUT gene family in pubertal pollen of rice by RT-PCR and laser micro-dissection. In five SUT genes, ''OsSUT1'' and ''OsSUT3'' expressed in pollen, while the expression of both have time specificity and are not identical. ''OsSUT1'' are expressed in pollen, determined by using ''OsSUT1'' promoter drive GUS expression. In order to further study ''OsSUT1'' function, we found insertion mutants.Tthe homozygous mutants (''SUT1'' - / -) can’t be obtained Heterozygous mutants (''SUT1'' + / -) have grain with normal grouting. However, the lose of ''OsSUT1'' does not affect the starch accumulation in the process of pollen development, suggesting that there may be other sucrose transporters involved in starch biosynthesis (Hirose et al., 2010).

Five sucrose transport gene of rice are expressed in xenopus oocyte and determined by the method of electrophysiology for their activities. It showed that OsSUT1 and OsSUT5 can generate sucrose-dependent electricity through the two electrode voltage clamp technique analysis. We tested the dynamic character of sucrose transport, substrate specificity, PH dependency and sucrose K0.5. OsSUT1 show the similar characteristics with ⅡSUT of all monocotyledon detected before: K0.5 is 7.50 mM, the optimal pH5.6. Compared with OsSUT1, substrate affinity of OsSUT5 is slightly higher (K0.5 is 2.32 mM, the optimal pH5.6). When compared with all Ⅱ SUTs before, substrate specificity of OsSUT5 is a little poorer, pH dependence lower. Meanwhile, the authour detected the regulation of reduced glutathione GSH and oxidized glutathione GSSG for each OsSUTs and ZmSUT1 in corn and HvSUT1 in barley, and found GSH and GSSG had no significant effect on these SUTs activity in xenopus oocyte. In conclusion, transport active differences between OsSUT1 and OsSUT5 has certain scope, which may be adapted to their functions in plants (Sun et al., 2010)

===Expression===
Please input expression information here.
''OsSUT1'' is expressed highly in the germination of the seed source and ears, lowly in roots (Aoki et al., 2003).

GUS expression drived by OsSUT1 promoter and immune positioning with OsSUT1 shows that, in the process of grouting, GUS activity and OsSUT1 protein appeared in the mature phloem organized of all the nutrients involved in the assimilation product long-distance transportation way. In addition, OsSUT1 is detected expressing in flag foliage and leaf sheath before earing. GUS activity mainly appeared in the phloem companion cell cell, while its protein in the companion cell and molecular sieve tube. （Scofield et al., 2007）

In five SUT genes, ''OsSUT1'' and ''OsSUT3'' expressed in pollen, while the expression of both have time specificity and are not identical. ''OsSUT1'' are expressed in pollen, determined by using ''OsSUT1'' promoter drive GUS expression. （Hirose et al., 2010）

===Evolution===
Please input evolution information here.
Using ''OsSUT1'' and ''HvSUT2'' blast rice genome and get four SUTs, ''OsSUT2'', ''OsSUT3'', ''OsSUT4'' and ''OsSUT5''. These four genes and ''OsSUT1'' constitutes the sucrose transporters of rice gene families. The structure prediction of proteins translated from five SUT genes show that all have 12 transmembrane helical and a domain structure highly conserved in all known SUTs, suggesting the four ''SUTs'' coding functional sucrose transporters.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

1. National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602 Japan.

2. Hokuriku National Agricultural Experiment Station, 1-2-1 Inada, Joetsu, Niigata, 943-0193 Japan.

3. Hokuriku Research Center, National Agricultural Research Center, National Agriculture and Food Research Organization, Joetsu, Niigata 943-0193, Japan.

4. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou, Jiangsu, China.

5. Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.

6. CSIRO Plant Industry, Canberra, ACT 2601, Australia.

7. Department of Rice Research, National Agricultural Research Center, Joetsu, Niigata, 943-0193 Japan.

8. Shahid Chamran University, College of Agriculture, Department of Agronomy and Plant Breeding, P.O. Box 61357-83151, Ahwaz, Iran.

9. University of California, San Diego, Division of Biological Sciences, Section of Cell and Developmental Biology, 9500 Gilman Drive #0116, Muir Biology Building, La Jolla, CA 92093, USA.

==References==
Please input cited references here.
1. Mohammad R. Siahpoosh;Diego H. Sanchez;Armin Schlereth;Graham N. Scofield;Robert T. Furbank;Joost T. van Dongen;Joachim Kopka
Modification of OsSUT1 gene expression modulates the salt response of rice Oryza sativa cv. Taipei 309
Plant Science, 2012, 182: 101-111
2. Tatsuro Hirose;Zujian Zhang;Akio Miyao;Hirohiko Hirochika;Ryu Ohsugi;Tomio Terao
Disruption of a gene for rice sucrose transporter, OsSUT1, impairs pollen function but pollen maturation is unaffected
Journal of Experimental Botany, 2010, 61(13): 3639-3646
3. Ye Sun;Anke Reinders;Kathryn R. LaFleur;Toko Mori;John M. Ward
Transport Activity of Rice Sucrose Transporters OsSUT1 and OsSUT5
Plant and Cell Physiology, 2010, 51(1): 114-122
4. Graham N. Scofield;Naohiro Aoki;Tatsuro Hirose;Makoto Takano;Colin L. D. Jenkins;Robert T. Furbank
The role of the sucrose transporter, OsSUT1, in germination and early seedling growth and development of rice plants
Journal of Experimental Botany, 2007, 58(3): 483-495
5. Graham N. Scofield;Tatsuro Hirose;Naohiro Aoki;Robert T. Furbank
Involvement of the sucrose transporter, OsSUT1, in the long-distance pathway for assimilate transport in rice
Journal of Experimental Botany, 2007, 58(12): 3155-3169
6. Naohiro Aoki;Tatsuro Hirose;Graham N. Scofield;Paul R. Whitfeld;Robert T. Furbank
The Sucrose Transporter Gene Family in Rice
Plant and Cell Physiology, 2003, 44(3): 223-232
7. Graham N. Scofield;Tatsuro Hirose;Judy A. Gaudron;Robert T. Furbank;Narayana M. Upadhyaya;Ryu Ohsugi
Antisense suppression of the rice transporter gene, OsSUT1, leads to impaired grain filling and germination but does not affect photosynthesis
Functional Plant Biology, 2002, 29(7): 815-826
8. Ken Ishimaru;Tatsurou Hirose;Naohiro Aoki;Sakiko Takahashi;Kiyomi Ono;Shinichi Yamamoto;Jiangzhong Wu;Shoko Saji;Tomoya Baba;Masashi Ugaki;Takashi Matsumoto;Ryu Ohsugi
Antisense Expression of a Rice Sucrose Transporter OsSUT1 in Rice (Oryza sativa L.)
Plant and Cell Physiology, 2001, 42(10): 1181-1185

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0170900|
Description = Sucrose transporter|
Version = NM_001055646.1 GI:115451020 GeneID:4331775|
Length = 6326 bp|
Definition = Oryza sativa Japonica Group Os03g0170900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:3776143..3782468|
CDS = 3776388..3776561,3776678..3776721,3776805..3776868,3776971..3777204,3777314..3777457 ,3777576..3777770,3777996..3778071,3778238..3778337,3778504..3778537 ,3778625..3778709,3778775..3778839,3778929..3779027,3779359..3779424 ,3782034..3782270|
GCID = <gbrowseImage1>
name=NC_008396:3776143..3782468
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:3776143..3782468
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctcgcggcagcggggccggaggaggcggcggcggtggtggcggggggctggagctgtcggtcggggtcggcggcggcggcggcgcgcgtggtgggggaggcggtgaggcggcggcggcggtggagacggcggcgccgatcagcctcgggaggctcatcctctccgggatggtcgccggcggcgtgcagtacgggtgggcgctccagctctccctcctcaccccctacgtccagacactcggactgtcgcatgctcttacttcattcatgtggctctgtggtcctattgctggcatggtggttcaaccatgcgtgggtctatacagtgataggtgcacctcaaaatggggaagacgaaggccatatattcttacaggatgtgtgttgatatgcttggctgtcgtagttattggattttcagctgacattggatacgccatgggtgatacaaaggaagactgcagtgtctaccatggatcacggtggcatgctgctattgtatatgtcctgggattctggcttcttgatttttccaacaacactgtgcagggtccagcacgtgctctaatggctgatttatcaggacgtcatggacctggcacagctaactccatcttctgttcttggatggcaatgggaaacatcctaggatactcctctggttccacaaacaattggcacaagtggtttccattccttaaaacaagagcttgctgtgaagcttgtgcaaatttgaaaggtgcatttctggtggctgtgattttcctgtccctgtgcttggttataactctgatatttgccaaggaagttccattcaaaggaaatgcagccctcccaacaaaatcaaacgagccagctgagcctgaaggcactggtccactagcagtgttgaaaggcttcaggaacttacctactgggatgccttctgtgcttattgtgactggccttacctggttgtcttggttcccattcatcctctacgacactgactggatgggccgtgagatctaccacggtgacccaaagggaactgatcctcagatcgaggctttcaaccagggtgtcagggcaggtgcatttggcctgctactgaattcgattgtgctaggattcagctcattcttgattgaaccaatgtgccggaaagtcgggccaagggtggtgtgggtgacgagcaatttcctcgtctgcatcgccatggctgcaactgcactgatcagcttctggtcactcaaggacttccatggaactgttcagaaagccatcactgcagacaagagcatcaaggctgtctgcctcgtcctctttgcgtttcttggagtccctctcgccgttctgtacagtgttcccttcgcagtgacggcgcaattagcggccactagagggggtggccaagggctctgcaccggcgtcctcaacatctccatcgtcatccctcaggtggtcatcgcgctgggagccgggccgtgggacgagctgttcgggaaggggaacatcccggcgttcggcctcgcctcgggattcgcgctcatcggcggcgtcgccggaatattcctgctgcccaagatctccaagcgccagttccggtcggtcagcatgggcggcggtcactga</cdnaseq>|
AA = <aaseq>MARGSGAGGGGGGGGGGLELSVGVGGGGGARGGGGGEAAAAVET AAPISLGRLILSGMVAGGVQYGWALQLSLLTPYVQTLGLSHALTSFMWLCGPIAGMVV QPCVGLYSDRCTSKWGRRRPYILTGCVLICLAVVVIGFSADIGYAMGDTKEDCSVYHG SRWHAAIVYVLGFWLLDFSNNTVQGPARALMADLSGRHGPGTANSIFCSWMAMGNILG YSSGSTNNWHKWFPFLKTRACCEACANLKGAFLVAVIFLSLCLVITLIFAKEVPFKGN AALPTKSNEPAEPEGTGPLAVLKGFRNLPTGMPSVLIVTGLTWLSWFPFILYDTDWMG REIYHGDPKGTDPQIEAFNQGVRAGAFGLLLNSIVLGFSSFLIEPMCRKVGPRVVWVT SNFLVCIAMAATALISFWSLKDFHGTVQKAITADKSIKAVCLVLFAFLGVPLAVLYSV PFAVTAQLAATRGGGQGLCTGVLNISIVIPQVVIALGAGPWDELFGKGNIPAFGLASG FALIGGVAGIFLLPKISKRQFRSVSMGGGH</aaseq>|
DNA = <dnaseqindica>5908..6081#5748..5791#5601..5664#5265..5498#5012..5155#4699..4893#4398..4473#4132..4231#3932..3965#3760..3844#3630..3694#3442..3540#3045..3110#199..435#aaacacaaacccaccacctcctcctcctcctcccatccagcacgcgcctcctctctcgcgcggctttccatttccatctccccctcctcctcctacgtctccgccgctcctcacttcctccactcgatttcctttcttggcctctcctcctctgacacaggggtgtgcaggtttgtgtttgtgcgtggcgcgtccgccatggctcgcggcagcggggccggaggaggcggcggcggtggtggcggggggctggagctgtcggtcggggtcggcggcggcggcggcgcgcgtggtgggggaggcggtgaggcggcggcggcggtggagacggcggcgccgatcagcctcgggaggctcatcctctccgggatggtcgccggcggcgtgcagtacgggtgggcgctccagctctccctcctcaccccctacgtccaggtatatatatatatatactgtactcctgtgtattttgcacactctcatgcatgcatctgaaactctgaataatcctgcaaaaaatatatacaagtatctcacgtgtggatttgattttgttcgcgagtttttgctcgtgctagctgttctaatctgcaaggtttcatggtagtagattatatatgtcctggttacaatcttttcaaggttataatcttgtaatttttttctgctttgtcaatatttacttcgcactgtctggtgtgagtcgttaaaaaaatatatatcttgaaagattggggaatttttcttcatgtgtggggagagatttatttttttttgtttgaacggtagatttgactagtgtctaaaagtaccgtaataaattcaatctgcatgccttttgtaattttttttgctcatgtgtttatatacatgtggccaaatatgcatttgatgcactagctatcatttttgtcattttgtagttattccttttcctttcctttttttttcctttttctttttgttgtcactagaggattcatatcctctcgattcctctatgaaacgtcggcgttttctgcctcttttctgaagaatctgtatacctatataagaatcaaaatcttttataagttctttttgtgcactttggatcaatcaggctagccagcatgacacttagcatcagtaccggtctactgtaccgagattcactttacgcttgacacgtttctcaactccttgtttgattccattttacctcaattagactgtttgattccgttttatctcagatatgacactcctttttttaaaattccattttacctcagatgctgtacagtgccataaaaaaatatacatctgctccaactcaccttatccttcttgtaataaccacgtattaggttaatagctactaatagctagagatgtaaacaaaaataagcagacaagggcaaaagtgagcggtaataatggagaattaatctgtcgttcctccgcgcgcagttaaaggtaggcagatgacgggtccctcccctctattccatactaaccgcaagatgaaactaatattcttactgctagatatataaatcatccttcatttagcttcatctccctccttaagatgtgttcttttcttccatttcctaacttctactcccttcgtttgacgtacacatatttcctaaactgccaaacgatgtgtttctactaaaaatttatatggtttaaaaaacatattaatatatttttttttgtctttttagctaatatttaattaattatgtgctaaactaccgctcaattttgtgtaccgggagaaagggttctcaatcattacaaaagaacacagcctagtctccatgaaaaacatcacttttattttaagacagagcaagtggagcgattttataactttactatagtacaaaattaatggtcagaaatttaaaattttgaccgaattttatcgtaagcatcaaatatttattaccggagagatagagtattggttatttgcctctctgtcgtcaaatattctaaacaaagacgatcttcttagtggttaaacttaaatctcagtccttccaggcgtttatttgaaggatttatagcggagataaacagctccttttttttcggaaatacgtaaggagggcacttttgtattaaggggaataaaaagggtctgtacagggaaaaccaaaaaacagcaggggacaggaataaacagctcctcacttcctctttgtatcagtccatactaattaacaaagtgttccaaaatgttctgtactttttctgtagaacattatatgcagttcatcacccctgatggtccatgcatcaggtctagctacctactgttgtattgaagtatttctggcgaagtttatccttaccttagccttctcgagccaagtgccataaagatggttgctgtagaggcaactccaacccactttaaccgcgcacatgaaaaataaaggggtaaaacaaggaaattctaggtcagatatatatgtcctgtggactgtggcacagttgcaacagagcaattgcataggcaaatgagggcagatcatcatgtacccatagcctgttggtgctatgttagcatccaagcattgccattttaactattaagattcttcttcttatatttgacaagtgaaataaatttagtgactgaacatatatagaacgatgaacagtctttgtatgtttattcatgccatgggggaaagagaagtgtgatctgaagttattggatagatagctgttagttgtagcatgcaaggcaatgcatcctggaaccaacagaccaacagagctggggatatttcatctgctaaatttctgggaaaatttattgtgtggtaatcaatatgtgttcactctttgcaactggtacacgctctctgatgtttctgaatatcttccacagtgatgcagatcttgttgacacagcaaactcaacaaacatagtcatttttttaaggaatgaagcatgatcatctattgtcagagtagattacagtcaaagtttttttataagccaaaacaactgattttctaacctctgttatttacagacactcggactgtcgcatgctcttacttcattcatgtggctctgtggtcctattgctggcatggtggtaagtaacaataccaaattatctgctctatcgatatatacgagacaaagcttttgccctcttttaaaaaaaataaatcaaattaattcatgttatggaatctgtactttctgcaatgtaaattcaagttactttagtgtcttcactacaacaaaaacttgggtatcctgaactcctgtatctatcagagtttgaagctgcattttctgtagcttctgatatgtgtaatgtagatcatactgtcataattcataagctttcataaaaaatgtttgtgtacaagtcatattttttttaattcttgatccattatcatctcccaatcctgcaggttcaaccatgcgtgggtctatacagtgataggtgcacctcaaaatggggaagacgaaggccatatattcttacaggatgtgtgttgatatgcttggctgtaagaaccactgaaccttggaactcttattccttaagttgaagtccccaaattttctctcatattgacattgcactttatgtatgtaggtcgtagttattggattttcagctgacattggatacgccatgggtgatacaaaggaagactgcaggtaactgaacattttgcgtatcataattagcagataacagttatctgatacaccatgtaatgcagtgtctaccatggatcacggtggcatgctgctattgtatatgtcctgggattctggcttcttgatttttccaacaacactgtgcaggttagtgcctactatataataatcatgttaaagaaatttgtacaaagaatcataacaaaaatcttaattttgcaatgcaatttctagggtccagcacgtgctctaatggctgatttatcaggtaatttttcatagccttgtctttcagagttaactaccaaatttttattggtttccacagttcactaacacttgattgattccagaaaaaaaaaagttgcccttgtgttctgggcttctggtatcagtgggattttggatttgatctcctaattttcgttctgtaggacgtcatggacctggcacagctaactccatcttctgttcttggatggcaatgggaaacatcctaggatactcctctggttccacaaacaattggcacaagtaagttactcttatgtatcagtttcatacagattttctgtttagcacagtctattcagttgcattcttttctgaaagaaggcaagtgaaattcatattacattagtagagaaggtgaaatttgtttacaagtattgagttgcattctccctaattaattgcgcaggtggtttccattccttaaaacaagagcttgctgtgaagcttgtgcaaatttgaaaggtgcatttctggtggctgtggtaagttctgttattacctcataaactgcctgctgataatactttaacaatgtgctaatattagtctttgtaataagatagtactatactgaaaatattttagcgagtatgagtaatttaacttacatattgtattgctgttcctcttttttcaaccctgtcatattggttgctttttttcacagcctaacatgctcttgtttggtcattttcccctgttttcagattttcctgtccctgtgcttggttataactctgatatttgccaaggaagttccattcaaaggaaatgcagccctcccaacaaaatcaaacgagccagctgagcctgaaggcactggtccactagcagtgttgaaaggcttcaggaacttacctactgggatgccttctgtgcttattgtgactggccttacctgggtatgcaatatacatcagaaaaagaacttttcagattattgaaaaaattgctactagatcatatcttagttatatcttatagtaccttaccttttctatgatatgattgtccttgtagttgtcttggttcccattcatcctctacgacactgactggatgggccgtgagatctaccacggtgacccaaagggaactgatcctcagatcgaggctttcaaccagggtgtcagggcaggtgcatttggcctgctactgaattcggtaagatctgattggtttctgaattctgattgccacaactactgtcaaatctttaacactcaagccatgttctttggcagcaactaaccatgggcttctgatcgtgcagattgtgctaggattcagctcattcttgattgaaccaatgtgccggaaagtcgggccaagggtggtgtgggtgacgagcaatttcctcgtctgcatcgccatggctgcaactgcactgatcagcttctggtcactcaaggacttccatggaactgttcagaaagccatcactgcagacaagagcatcaaggctgtctgcctcgtcctctttgcgtttcttggagtccctctcgccgtaagaatcactgacacttcaacttaaacacattgctaaaaaaaattatttcctcaagaatgccttatcctgatgaactgtctgaaaatttgcgcgatgcaggttctgtacagtgttcccttcgcagtgacggcgcaattagcggccactagagggggtggccaaggtatgaatcccaacattcttgcagcttttcttgacataattcggtgttgtttggatgctggtctaatccatggcgatctgcagggctctgcaccggcgtcctcaacatctccatcgtcatccctcaggttaattagctgccaaattctaatccgtttcccttgaaaccgaatcagatatacttcatggattattagaatttgaatgctcatggattcatggttgcgtggtgttcttccctcaggtggtcatcgcgctgggagccgggccgtgggacgagctgttcgggaaggggaacatcccggcgttcggcctcgcctcgggattcgcgctcatcggcggcgtcgccggaatattcctgctgcccaagatctccaagcgccagttccggtcggtcagcatgggcggcggtcactgaccgccccgctctcgccgccggctgcggcggcggcggcggccggagcagcgccgccggaagtggtgagctttgtagttctcgtttgcaatggatcaaaggagccccctctttttttttacatagggtaattaattaattaaggctgttaattaggaaggggtgaggtggccctgcagctggggcccacgctttgttgccacctcggtagaagagaataagcagatcatgcagataatatggaactg</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055646.1 RefSeq:Os03g0170900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

Os03g0170900

2014-06-10T10:06:49Z

Sean: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.
It's the first sucrose transporter gene in rice, rice sucrose transport gene, named ''OsSUT1''. It might not play an important role in carbon metabolism of rice, but participate in the long distance transportation of assimilation product, and plays an important role in the process of sucrose by apoplast pathway.
The expression changes of ''OsSUT1'' gene adjusted salt stress response of the Taipei 309 rice varieties (Siahpoosh et al. 2012).

OsSUT1 didn’t directly involve in sucrose transport between epithelial cells in seed scutellum or between aleurone cells during the process of germination. However, in the companion cells and keratin vascular bundle, OsSUT1 may participate in the sucrose loading of phloems to transport to the developing buds and roots. OsSUT1 may also participate in regaining sucrose from the phloem tissue of taproots, coleoptiles and first and second leaves and other parts (Scofield et al., 2007).

Rice sucrose transporters OsSUT1, participate in transport of assimilation product over long distances. OsSUT1 function has been previously tested in the germination and early seedling growth. In this article, the author tested the role of OsSUT1 in assimilate during the whole transport over long distances (from flag blades to the base of the grouting seeds). GUS expression drived by OsSUT1 promoter and immune positioning with OsSUT1 shows that, in the process of grouting, GUS activity and OsSUT1 protein appeared in the mature phloem organized of all the nutrients involved in the assimilation product long-distance transportation way. In addition, OsSUT1 is detected expressing in flag foliage and leaf sheath before earing. GUS activity mainly appeared in the phloem companion cell cell, while its protein in the companion cell and molecular sieve tube. RT-PCR detection showed OsSUT1 transcriptions expressed in the toppest internode (internode 1). The location datas of OsSUT1 is concerned with the starch and sucrose content in these organizations and carbohydrates stored in the stems. In order to detect the connection between the cells, we proceeded the feeding dyeing experiments. The results showed that the symplast between blades of flag leaves, leaf sheath and internode 1 phloem have continuity with parenchyma cells around. Therefore, it’s believed that, during the process of sucrose transport, OsSUT1 may participate in recycling spilled sucrose (Scofield et al., 2007).

Destruction of rice sucrose transporters OsSUT1 damage the function of pollen but does not affect the mature of pollen. As is known to all, in all kinds of sucrose transport steps, sucrose transporters (SUTs) plays an important role in the process of sucrose by apoplast pathway. Due to the developing pollen is symplast separated withanther tissue, SUTs may play a role in the process of sucrose by apoplast pathway. In order to verify this possibility, we anlyze SUT gene family in pubertal pollen of rice by RT-PCR and laser micro-dissection. In five SUT genes, ''OsSUT1'' and ''OsSUT3'' expressed in pollen, while the expression of both have time specificity and are not identical. ''OsSUT1'' are expressed in pollen, determined by using ''OsSUT1'' promoter drive GUS expression. In order to further study ''OsSUT1'' function, we found insertion mutants.Tthe homozygous mutants (''SUT1'' - / -) can’t be obtained Heterozygous mutants (''SUT1'' + / -) have grain with normal grouting. However, the lose of ''OsSUT1'' does not affect the starch accumulation in the process of pollen development, suggesting that there may be other sucrose transporters involved in starch biosynthesis (Hirose et al., 2010).

Five sucrose transport gene of rice are expressed in xenopus oocyte and determined by the method of electrophysiology for their activities. It showed that OsSUT1 and OsSUT5 can generate sucrose-dependent electricity through the two electrode voltage clamp technique analysis. We tested the dynamic character of sucrose transport, substrate specificity, PH dependency and sucrose K0.5. OsSUT1 show the similar characteristics with ⅡSUT of all monocotyledon detected before: K0.5 is 7.50 mM, the optimal pH5.6. Compared with OsSUT1, substrate affinity of OsSUT5 is slightly higher (K0.5 is 2.32 mM, the optimal pH5.6). When compared with all Ⅱ SUTs before, substrate specificity of OsSUT5 is a little poorer, pH dependence lower. Meanwhile, the authour detected the regulation of reduced glutathione GSH and oxidized glutathione GSSG for each OsSUTs and ZmSUT1 in corn and HvSUT1 in barley, and found GSH and GSSG had no significant effect on these SUTs activity in xenopus oocyte. In conclusion, transport active differences between OsSUT1 and OsSUT5 has certain scope, which may be adapted to their functions in plants (Sun et al., 2010)

===Expression===
Please input expression information here.
''OsSUT1'' is expressed highly in the germination of the seed source and ears, lowly in roots (Aoki et al., 2003).

GUS expression drived by OsSUT1 promoter and immune positioning with OsSUT1 shows that, in the process of grouting, GUS activity and OsSUT1 protein appeared in the mature phloem organized of all the nutrients involved in the assimilation product long-distance transportation way. In addition, OsSUT1 is detected expressing in flag foliage and leaf sheath before earing. GUS activity mainly appeared in the phloem companion cell cell, while its protein in the companion cell and molecular sieve tube. （Scofield et al., 2007）

In five SUT genes, ''OsSUT1'' and ''OsSUT3'' expressed in pollen, while the expression of both have time specificity and are not identical. ''OsSUT1'' are expressed in pollen, determined by using ''OsSUT1'' promoter drive GUS expression. （Hirose et al., 2010）

===Evolution===
Please input evolution information here.
Using ''OsSUT1'' and ''HvSUT2'' blast rice genome and get four SUTs, ''OsSUT2'', ''OsSUT3'', ''OsSUT4'' and ''OsSUT5''. These four genes and ''OsSUT1'' constitutes the sucrose transporters of rice gene families. The structure prediction of proteins translated from five SUT genes show that all have 12 transmembrane helical and a domain structure highly conserved in all known SUTs, suggesting the four ''SUTs'' coding functional sucrose transporters.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.
1. National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602 Japan
2. Hokuriku National Agricultural Experiment Station, 1-2-1 Inada, Joetsu, Niigata, 943-0193 Japan
3. Hokuriku Research Center, National Agricultural Research Center, National Agriculture and Food Research Organization, Joetsu, Niigata 943-0193, Japan
4. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou, Jiangsu, China.
5. Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan
6. CSIRO Plant Industry, Canberra, ACT 2601, Australia.
7. Department of Rice Research, National Agricultural Research Center, Joetsu, Niigata, 943-0193 Japan.
8. Shahid Chamran University, College of Agriculture, Department of Agronomy and Plant Breeding, P.O. Box 61357-83151, Ahwaz, Iran.
9. University of California, San Diego, Division of Biological Sciences, Section of Cell and Developmental Biology, 9500 Gilman Drive #0116, Muir Biology Building, La Jolla, CA 92093, USA.

==References==
Please input cited references here.
1. Mohammad R. Siahpoosh;Diego H. Sanchez;Armin Schlereth;Graham N. Scofield;Robert T. Furbank;Joost T. van Dongen;Joachim Kopka
Modification of OsSUT1 gene expression modulates the salt response of rice Oryza sativa cv. Taipei 309
Plant Science, 2012, 182: 101-111
2. Tatsuro Hirose;Zujian Zhang;Akio Miyao;Hirohiko Hirochika;Ryu Ohsugi;Tomio Terao
Disruption of a gene for rice sucrose transporter, OsSUT1, impairs pollen function but pollen maturation is unaffected
Journal of Experimental Botany, 2010, 61(13): 3639-3646
3. Ye Sun;Anke Reinders;Kathryn R. LaFleur;Toko Mori;John M. Ward
Transport Activity of Rice Sucrose Transporters OsSUT1 and OsSUT5
Plant and Cell Physiology, 2010, 51(1): 114-122
4. Graham N. Scofield;Naohiro Aoki;Tatsuro Hirose;Makoto Takano;Colin L. D. Jenkins;Robert T. Furbank
The role of the sucrose transporter, OsSUT1, in germination and early seedling growth and development of rice plants
Journal of Experimental Botany, 2007, 58(3): 483-495
5. Graham N. Scofield;Tatsuro Hirose;Naohiro Aoki;Robert T. Furbank
Involvement of the sucrose transporter, OsSUT1, in the long-distance pathway for assimilate transport in rice
Journal of Experimental Botany, 2007, 58(12): 3155-3169
6. Naohiro Aoki;Tatsuro Hirose;Graham N. Scofield;Paul R. Whitfeld;Robert T. Furbank
The Sucrose Transporter Gene Family in Rice
Plant and Cell Physiology, 2003, 44(3): 223-232
7. Graham N. Scofield;Tatsuro Hirose;Judy A. Gaudron;Robert T. Furbank;Narayana M. Upadhyaya;Ryu Ohsugi
Antisense suppression of the rice transporter gene, OsSUT1, leads to impaired grain filling and germination but does not affect photosynthesis
Functional Plant Biology, 2002, 29(7): 815-826
8. Ken Ishimaru;Tatsurou Hirose;Naohiro Aoki;Sakiko Takahashi;Kiyomi Ono;Shinichi Yamamoto;Jiangzhong Wu;Shoko Saji;Tomoya Baba;Masashi Ugaki;Takashi Matsumoto;Ryu Ohsugi
Antisense Expression of a Rice Sucrose Transporter OsSUT1 in Rice (Oryza sativa L.)
Plant and Cell Physiology, 2001, 42(10): 1181-1185

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0170900|
Description = Sucrose transporter|
Version = NM_001055646.1 GI:115451020 GeneID:4331775|
Length = 6326 bp|
Definition = Oryza sativa Japonica Group Os03g0170900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:3776143..3782468|
CDS = 3776388..3776561,3776678..3776721,3776805..3776868,3776971..3777204,3777314..3777457 ,3777576..3777770,3777996..3778071,3778238..3778337,3778504..3778537 ,3778625..3778709,3778775..3778839,3778929..3779027,3779359..3779424 ,3782034..3782270|
GCID = <gbrowseImage1>
name=NC_008396:3776143..3782468
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:3776143..3782468
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctcgcggcagcggggccggaggaggcggcggcggtggtggcggggggctggagctgtcggtcggggtcggcggcggcggcggcgcgcgtggtgggggaggcggtgaggcggcggcggcggtggagacggcggcgccgatcagcctcgggaggctcatcctctccgggatggtcgccggcggcgtgcagtacgggtgggcgctccagctctccctcctcaccccctacgtccagacactcggactgtcgcatgctcttacttcattcatgtggctctgtggtcctattgctggcatggtggttcaaccatgcgtgggtctatacagtgataggtgcacctcaaaatggggaagacgaaggccatatattcttacaggatgtgtgttgatatgcttggctgtcgtagttattggattttcagctgacattggatacgccatgggtgatacaaaggaagactgcagtgtctaccatggatcacggtggcatgctgctattgtatatgtcctgggattctggcttcttgatttttccaacaacactgtgcagggtccagcacgtgctctaatggctgatttatcaggacgtcatggacctggcacagctaactccatcttctgttcttggatggcaatgggaaacatcctaggatactcctctggttccacaaacaattggcacaagtggtttccattccttaaaacaagagcttgctgtgaagcttgtgcaaatttgaaaggtgcatttctggtggctgtgattttcctgtccctgtgcttggttataactctgatatttgccaaggaagttccattcaaaggaaatgcagccctcccaacaaaatcaaacgagccagctgagcctgaaggcactggtccactagcagtgttgaaaggcttcaggaacttacctactgggatgccttctgtgcttattgtgactggccttacctggttgtcttggttcccattcatcctctacgacactgactggatgggccgtgagatctaccacggtgacccaaagggaactgatcctcagatcgaggctttcaaccagggtgtcagggcaggtgcatttggcctgctactgaattcgattgtgctaggattcagctcattcttgattgaaccaatgtgccggaaagtcgggccaagggtggtgtgggtgacgagcaatttcctcgtctgcatcgccatggctgcaactgcactgatcagcttctggtcactcaaggacttccatggaactgttcagaaagccatcactgcagacaagagcatcaaggctgtctgcctcgtcctctttgcgtttcttggagtccctctcgccgttctgtacagtgttcccttcgcagtgacggcgcaattagcggccactagagggggtggccaagggctctgcaccggcgtcctcaacatctccatcgtcatccctcaggtggtcatcgcgctgggagccgggccgtgggacgagctgttcgggaaggggaacatcccggcgttcggcctcgcctcgggattcgcgctcatcggcggcgtcgccggaatattcctgctgcccaagatctccaagcgccagttccggtcggtcagcatgggcggcggtcactga</cdnaseq>|
AA = <aaseq>MARGSGAGGGGGGGGGGLELSVGVGGGGGARGGGGGEAAAAVET AAPISLGRLILSGMVAGGVQYGWALQLSLLTPYVQTLGLSHALTSFMWLCGPIAGMVV QPCVGLYSDRCTSKWGRRRPYILTGCVLICLAVVVIGFSADIGYAMGDTKEDCSVYHG SRWHAAIVYVLGFWLLDFSNNTVQGPARALMADLSGRHGPGTANSIFCSWMAMGNILG YSSGSTNNWHKWFPFLKTRACCEACANLKGAFLVAVIFLSLCLVITLIFAKEVPFKGN AALPTKSNEPAEPEGTGPLAVLKGFRNLPTGMPSVLIVTGLTWLSWFPFILYDTDWMG REIYHGDPKGTDPQIEAFNQGVRAGAFGLLLNSIVLGFSSFLIEPMCRKVGPRVVWVT SNFLVCIAMAATALISFWSLKDFHGTVQKAITADKSIKAVCLVLFAFLGVPLAVLYSV PFAVTAQLAATRGGGQGLCTGVLNISIVIPQVVIALGAGPWDELFGKGNIPAFGLASG FALIGGVAGIFLLPKISKRQFRSVSMGGGH</aaseq>|
DNA = 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gcttcaggaacttacctactgggatgccttctgtgcttattgtgactggccttacctgggtatgcaatatacatcagaaaaagaacttttcagattattgaaaaaattgctactagatcatatcttagttatatcttatagtaccttaccttttctatgatatgattgtccttgtagttgtcttggttcccattcatcctctacgacactgactggatgggccgtgagatctaccacggtgacccaaagggaactgatcctcagatcgaggctttcaaccagggtgtcagggcaggtgcatttggcctgctactgaattcggtaagatctgattggtttctgaattctgattgccacaactactgtcaaatctttaacactcaagccatgttctttggcagcaactaaccatgggcttctgatcgtgcagattgtgctaggattcagctcattcttgattgaaccaatgtgccggaaagtcgggccaagggtggtgtgggtgacgagcaatttcctcgtctgcatcgccatggctgcaactgcactgatcagcttctggtcactcaaggacttccatggaactgttcagaaagccatcactgcagacaagagcatcaaggctgtctgcctcgtcctctttgcgtttcttggagtccctctcgccgtaagaatcactgacacttcaacttaaacacattgctaaaaaaaattatttcctcaagaatgccttatcctgatgaactgtctgaaaatttgcgcgatgcaggttctgtacagtgttcccttcgcagtgacggcgcaattagcggccactagagggggtggccaaggtatgaatcccaacattcttgcagcttttcttgacataattcggtgttgtttggatgctggtctaatccatggcgatctgcagggctctgcaccggcgtcctcaacatctccatcgtcatccctcaggttaattagctgccaaattctaatccgtttcccttgaaaccgaatcagatatacttcatggattattagaatttgaatgctcatggattcatggttgcgtggtgttcttccctcaggtggtcatcgcgctgggagccgggccgtgggacgagctgttcgggaaggggaacatcccggcgttcggcctcgcctcgggattcgcgctcatcggcggcgtcgccggaatattcctgctgcccaagatctccaagcgccagttccggtcggtcagcatgggcggcggtcactgaccgccccgctctcgccgccggctgcggcggcggcggcggccggagcagcgccgccggaagtggtgagctttgtagttctcgtttgcaatggatcaaaggagccccctctttttttttacatagggtaattaattaattaaggctgttaattaggaaggggtgaggtggccctgcagctggggcccacgctttgttgccacctcggtagaagagaataagcagatcatgcagataatatggaactg</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055646.1 RefSeq:Os03g0170900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

Os03g0170900

2014-06-10T03:01:08Z

Sean: /* References */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.
It's the first sucrose transporter gene in rice, rice sucrose transport gene, named ''OsSUT1''. It might not play an important role in carbon metabolism of rice, but participate in the long distance transportation of assimilation product, and plays an important role in the process of sucrose by apoplast pathway.
The expression changes of ''OsSUT1'' gene adjusted salt stress response of the Taipei 309 rice varieties (Siahpoosh et al. 2012).

OsSUT1 didn’t directly involve in sucrose transport between epithelial cells in seed scutellum or between aleurone cells during the process of germination. However, in the companion cells and keratin vascular bundle, OsSUT1 may participate in the sucrose loading of phloems to transport to the developing buds and roots. OsSUT1 may also participate in regaining sucrose from the phloem tissue of taproots, coleoptiles and first and second leaves and other parts (Scofield et al., 2007).

Rice sucrose transporters OsSUT1, participate in transport of assimilation product over long distances. OsSUT1 function has been previously tested in the germination and early seedling growth. In this article, the author tested the role of OsSUT1 in assimilate during the whole transport over long distances (from flag blades to the base of the grouting seeds). GUS expression drived by OsSUT1 promoter and immune positioning with OsSUT1 shows that, in the process of grouting, GUS activity and OsSUT1 protein appeared in the mature phloem organized of all the nutrients involved in the assimilation product long-distance transportation way. In addition, OsSUT1 is detected expressing in flag foliage and leaf sheath before earing. GUS activity mainly appeared in the phloem companion cell cell, while its protein in the companion cell and molecular sieve tube. RT-PCR detection showed OsSUT1 transcriptions expressed in the toppest internode (internode 1). The location datas of OsSUT1 is concerned with the starch and sucrose content in these organizations and carbohydrates stored in the stems. In order to detect the connection between the cells, we proceeded the feeding dyeing experiments. The results showed that the symplast between blades of flag leaves, leaf sheath and internode 1 phloem have continuity with parenchyma cells around. Therefore, it’s believed that, during the process of sucrose transport, OsSUT1 may participate in recycling spilled sucrose (Scofield et al., 2007).

Destruction of rice sucrose transporters OsSUT1 damage the function of pollen but does not affect the mature of pollen. As is known to all, in all kinds of sucrose transport steps, sucrose transporters (SUTs) plays an important role in the process of sucrose by apoplast pathway. Due to the developing pollen is symplast separated withanther tissue, SUTs may play a role in the process of sucrose by apoplast pathway. In order to verify this possibility, we anlyze SUT gene family in pubertal pollen of rice by RT-PCR and laser micro-dissection. In five SUT genes, ''OsSUT1'' and ''OsSUT3'' expressed in pollen, while the expression of both have time specificity and are not identical. ''OsSUT1'' are expressed in pollen, determined by using ''OsSUT1'' promoter drive GUS expression. In order to further study ''OsSUT1'' function, we found insertion mutants.Tthe homozygous mutants (''SUT1'' - / -) can’t be obtained Heterozygous mutants (''SUT1'' + / -) have grain with normal grouting. However, the lose of ''OsSUT1'' does not affect the starch accumulation in the process of pollen development, suggesting that there may be other sucrose transporters involved in starch biosynthesis (Hirose et al., 2010).

Five sucrose transport gene of rice are expressed in xenopus oocyte and determined by the method of electrophysiology for their activities. It showed that OsSUT1 and OsSUT5 can generate sucrose-dependent electricity through the two electrode voltage clamp technique analysis. We tested the dynamic character of sucrose transport, substrate specificity, PH dependency and sucrose K0.5. OsSUT1 show the similar characteristics with ⅡSUT of all monocotyledon detected before: K0.5 is 7.50 mM, the optimal pH5.6. Compared with OsSUT1, substrate affinity of OsSUT5 is slightly higher (K0.5 is 2.32 mM, the optimal pH5.6). When compared with all Ⅱ SUTs before, substrate specificity of OsSUT5 is a little poorer, pH dependence lower. Meanwhile, the authour detected the regulation of reduced glutathione GSH and oxidized glutathione GSSG for each OsSUTs and ZmSUT1 in corn and HvSUT1 in barley, and found GSH and GSSG had no significant effect on these SUTs activity in xenopus oocyte. In conclusion, transport active differences between OsSUT1 and OsSUT5 has certain scope, which may be adapted to their functions in plants (Sun et al., 2010)

===Expression===
Please input expression information here.
''OsSUT1'' is expressed highly in the germination of the seed source and ears, lowly in roots (Aoki et al., 2003).

GUS expression drived by OsSUT1 promoter and immune positioning with OsSUT1 shows that, in the process of grouting, GUS activity and OsSUT1 protein appeared in the mature phloem organized of all the nutrients involved in the assimilation product long-distance transportation way. In addition, OsSUT1 is detected expressing in flag foliage and leaf sheath before earing. GUS activity mainly appeared in the phloem companion cell cell, while its protein in the companion cell and molecular sieve tube. （Scofield et al., 2007）

In five SUT genes, ''OsSUT1'' and ''OsSUT3'' expressed in pollen, while the expression of both have time specificity and are not identical. ''OsSUT1'' are expressed in pollen, determined by using ''OsSUT1'' promoter drive GUS expression. （Hirose et al., 2010）

===Evolution===
Please input evolution information here.
Using ''OsSUT1'' and ''HvSUT2'' blast rice genome and get four SUTs, ''OsSUT2'', ''OsSUT3'', ''OsSUT4'' and ''OsSUT5''. These four genes and ''OsSUT1'' constitutes the sucrose transporters of rice gene families. The structure prediction of proteins translated from five SUT genes show that all have 12 transmembrane helical and a domain structure highly conserved in all known SUTs, suggesting the four ''SUTs'' coding functional sucrose transporters.

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==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.
1. Mohammad R. Siahpoosh;Diego H. Sanchez;Armin Schlereth;Graham N. Scofield;Robert T. Furbank;Joost T. van Dongen;Joachim Kopka
Modification of OsSUT1 gene expression modulates the salt response of rice Oryza sativa cv. Taipei 309
Plant Science, 2012, 182: 101-111
2. Tatsuro Hirose;Zujian Zhang;Akio Miyao;Hirohiko Hirochika;Ryu Ohsugi;Tomio Terao
Disruption of a gene for rice sucrose transporter, OsSUT1, impairs pollen function but pollen maturation is unaffected
Journal of Experimental Botany, 2010, 61(13): 3639-3646
3. Ye Sun;Anke Reinders;Kathryn R. LaFleur;Toko Mori;John M. Ward
Transport Activity of Rice Sucrose Transporters OsSUT1 and OsSUT5
Plant and Cell Physiology, 2010, 51(1): 114-122
4. Graham N. Scofield;Naohiro Aoki;Tatsuro Hirose;Makoto Takano;Colin L. D. Jenkins;Robert T. Furbank
The role of the sucrose transporter, OsSUT1, in germination and early seedling growth and development of rice plants
Journal of Experimental Botany, 2007, 58(3): 483-495
5. Graham N. Scofield;Tatsuro Hirose;Naohiro Aoki;Robert T. Furbank
Involvement of the sucrose transporter, OsSUT1, in the long-distance pathway for assimilate transport in rice
Journal of Experimental Botany, 2007, 58(12): 3155-3169
6. Naohiro Aoki;Tatsuro Hirose;Graham N. Scofield;Paul R. Whitfeld;Robert T. Furbank
The Sucrose Transporter Gene Family in Rice
Plant and Cell Physiology, 2003, 44(3): 223-232
7. Graham N. Scofield;Tatsuro Hirose;Judy A. Gaudron;Robert T. Furbank;Narayana M. Upadhyaya;Ryu Ohsugi
Antisense suppression of the rice transporter gene, OsSUT1, leads to impaired grain filling and germination but does not affect photosynthesis
Functional Plant Biology, 2002, 29(7): 815-826
8. Ken Ishimaru;Tatsurou Hirose;Naohiro Aoki;Sakiko Takahashi;Kiyomi Ono;Shinichi Yamamoto;Jiangzhong Wu;Shoko Saji;Tomoya Baba;Masashi Ugaki;Takashi Matsumoto;Ryu Ohsugi
Antisense Expression of a Rice Sucrose Transporter OsSUT1 in Rice (Oryza sativa L.)
Plant and Cell Physiology, 2001, 42(10): 1181-1185

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0170900|
Description = Sucrose transporter|
Version = NM_001055646.1 GI:115451020 GeneID:4331775|
Length = 6326 bp|
Definition = Oryza sativa Japonica Group Os03g0170900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:3776143..3782468|
CDS = 3776388..3776561,3776678..3776721,3776805..3776868,3776971..3777204,3777314..3777457 ,3777576..3777770,3777996..3778071,3778238..3778337,3778504..3778537 ,3778625..3778709,3778775..3778839,3778929..3779027,3779359..3779424 ,3782034..3782270|
GCID = <gbrowseImage1>
name=NC_008396:3776143..3782468
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:3776143..3782468
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctcgcggcagcggggccggaggaggcggcggcggtggtggcggggggctggagctgtcggtcggggtcggcggcggcggcggcgcgcgtggtgggggaggcggtgaggcggcggcggcggtggagacggcggcgccgatcagcctcgggaggctcatcctctccgggatggtcgccggcggcgtgcagtacgggtgggcgctccagctctccctcctcaccccctacgtccagacactcggactgtcgcatgctcttacttcattcatgtggctctgtggtcctattgctggcatggtggttcaaccatgcgtgggtctatacagtgataggtgcacctcaaaatggggaagacgaaggccatatattcttacaggatgtgtgttgatatgcttggctgtcgtagttattggattttcagctgacattggatacgccatgggtgatacaaaggaagactgcagtgtctaccatggatcacggtggcatgctgctattgtatatgtcctgggattctggcttcttgatttttccaacaacactgtgcagggtccagcacgtgctctaatggctgatttatcaggacgtcatggacctggcacagctaactccatcttctgttcttggatggcaatgggaaacatcctaggatactcctctggttccacaaacaattggcacaagtggtttccattccttaaaacaagagcttgctgtgaagcttgtgcaaatttgaaaggtgcatttctggtggctgtgattttcctgtccctgtgcttggttataactctgatatttgccaaggaagttccattcaaaggaaatgcagccctcccaacaaaatcaaacgagccagctgagcctgaaggcactggtccactagcagtgttgaaaggcttcaggaacttacctactgggatgccttctgtgcttattgtgactggccttacctggttgtcttggttcccattcatcctctacgacactgactggatgggccgtgagatctaccacggtgacccaaagggaactgatcctcagatcgaggctttcaaccagggtgtcagggcaggtgcatttggcctgctactgaattcgattgtgctaggattcagctcattcttgattgaaccaatgtgccggaaagtcgggccaagggtggtgtgggtgacgagcaatttcctcgtctgcatcgccatggctgcaactgcactgatcagcttctggtcactcaaggacttccatggaactgttcagaaagccatcactgcagacaagagcatcaaggctgtctgcctcgtcctctttgcgtttcttggagtccctctcgccgttctgtacagtgttcccttcgcagtgacggcgcaattagcggccactagagggggtggccaagggctctgcaccggcgtcctcaacatctccatcgtcatccctcaggtggtcatcgcgctgggagccgggccgtgggacgagctgttcgggaaggggaacatcccggcgttcggcctcgcctcgggattcgcgctcatcggcggcgtcgccggaatattcctgctgcccaagatctccaagcgccagttccggtcggtcagcatgggcggcggtcactga</cdnaseq>|
AA = <aaseq>MARGSGAGGGGGGGGGGLELSVGVGGGGGARGGGGGEAAAAVET AAPISLGRLILSGMVAGGVQYGWALQLSLLTPYVQTLGLSHALTSFMWLCGPIAGMVV QPCVGLYSDRCTSKWGRRRPYILTGCVLICLAVVVIGFSADIGYAMGDTKEDCSVYHG SRWHAAIVYVLGFWLLDFSNNTVQGPARALMADLSGRHGPGTANSIFCSWMAMGNILG YSSGSTNNWHKWFPFLKTRACCEACANLKGAFLVAVIFLSLCLVITLIFAKEVPFKGN AALPTKSNEPAEPEGTGPLAVLKGFRNLPTGMPSVLIVTGLTWLSWFPFILYDTDWMG REIYHGDPKGTDPQIEAFNQGVRAGAFGLLLNSIVLGFSSFLIEPMCRKVGPRVVWVT SNFLVCIAMAATALISFWSLKDFHGTVQKAITADKSIKAVCLVLFAFLGVPLAVLYSV PFAVTAQLAATRGGGQGLCTGVLNISIVIPQVVIALGAGPWDELFGKGNIPAFGLASG FALIGGVAGIFLLPKISKRQFRSVSMGGGH</aaseq>|
DNA = <dnaseqindica>5908..6081#5748..5791#5601..5664#5265..5498#5012..5155#4699..4893#4398..4473#4132..4231#3932..3965#3760..3844#3630..3694#3442..3540#3045..3110#199..435#aaacacaaacccaccacctcctcctcctcctcccatccagcacgcgcctcctctctcgcgcggctttccatttccatctccccctcctcctcctacgtctccgccgctcctcacttcctccactcgatttcctttcttggcctctcctcctctgacacaggggtgtgcaggtttgtgtttgtgcgtggcgcgtccgccatggctcgcggcagcggggccggaggaggcggcggcggtggtggcggggggctggagctgtcggtcggggtcggcggcggcggcggcgcgcgtggtgggggaggcggtgaggcggcggcggcggtggagacggcggcgccgatcagcctcgggaggctcatcctctccgggatggtcgccggcggcgtgcagtacgggtgggcgctccagctctccctcctcaccccctacgtccaggtatatatatatatatactgtactcctgtgtattttgcacactctcatgcatgcatctgaaactctgaataatcctgcaaaaaatatatacaagtatctcacgtgtggatttgattttgttcgcgagtttttgctcgtgctagctgttctaatctgcaaggtttcatggtagtagattatatatgtcctggttacaatcttttcaaggttataatcttgtaatttttttctgctttgtcaatatttacttcgcactgtctggtgtgagtcgttaaaaaaatatatatcttgaaagattggggaatttttcttcatgtgtggggagagatttatttttttttgtttgaacggtagatttgactagtgtctaaaagtaccgtaataaattcaatctgcatgccttttgtaattttttttgctcatgtgtttatatacatgtggccaaatatgcatttgatgcactagctatcatttttgtcattttgtagttattccttttcctttcctttttttttcctttttctttttgttgtcactagaggattcatatcctctcgattcctctatgaaacgtcggcgttttctgcctcttttctgaagaatctgtatacctatataagaatcaaaatcttttataagttctttttgtgcactttggatcaatcaggctagccagcatgacacttagcatcagtaccggtctactgtaccgagattcactttacgcttgacacgtttctcaactccttgtttgattccattttacctcaattagactgtttgattccgttttatctcagatatgacactcctttttttaaaattccattttacctcagatgctgtacagtgccataaaaaaatatacatctgctccaactcaccttatccttcttgtaataaccacgtattaggttaatagctactaatagctagagatgtaaacaaaaataagcagacaagggcaaaagtgagcggtaataatggagaattaatctgtcgttcctccgcgcgcagttaaaggtaggcagatgacgggtccctcccctctattccatactaaccgcaagatgaaactaatattcttactgctagatatataaatcatccttcatttagcttcatctccctccttaagatgtgttcttttcttccatttcctaacttctactcccttcgtttgacgtacacatatttcctaaactgccaaacgatgtgtttctactaaaaatttatatggtttaaaaaacatattaatatatttttttttgtctttttagctaatatttaattaattatgtgctaaactaccgctcaattttgtgtaccgggagaaagggttctcaatcattacaaaagaacacagcctagtctccatgaaaaacatcacttttattttaagacagagcaagtggagcgattttataactttactatagtacaaaattaatggtcagaaatttaaaattttgaccgaattttatcgtaagcatcaaatatttattaccggagagatagagtattggttatttgcctctctgtcgtcaaatattctaaacaaagacgatcttcttagtggttaaacttaaatctcagtccttccaggcgtttatttgaaggatttatagcggagataaacagctccttttttttcggaaatacgtaaggagggcacttttgtattaaggggaataaaaagggtctgtacagggaaaaccaaaaaacagcaggggacaggaataaacagctcctcacttcctctttgtatcagtccatactaattaacaaagtgttccaaaatgttctgtactttttctgtagaacattatatgcagttcatcacccctgatggtccatgcatcaggtctagctacctactgttgtattgaagtatttctggcgaagtttatccttaccttagccttctcgagccaagtgccataaagatggttgctgtagaggcaactccaacccactttaaccgcgcacatgaaaaataaaggggtaaaacaaggaaattctaggtcagatatatatgtcctgtggactgtggcacagttgcaacagagcaattgcataggcaaatgagggcagatcatcatgtacccatagcctgttggtgctatgttagcatccaagcattgccattttaactattaagattcttcttcttatatttgacaagtgaaataaatttagtgactgaacatatatagaacgatgaacagtctttgtatgtttattcatgccatgggggaaagagaagtgtgatctgaagttattggatagatagctgttagttgtagcatgcaaggcaatgcatcctggaaccaacagaccaacagagctggggatatttcatctgctaaatttctgggaaaatttattgtgtggtaatcaatatgtgttcactctttgcaactggtacacgctctctgatgtttctgaatatcttccacagtgatgcagatcttgttgacacagcaaactcaacaaacatagtcatttttttaaggaatgaagcatgatcatctattgtcagagtagattacagtcaaagtttttttataagccaaaacaactgattttctaacctctgttatttacagacactcggactgtcgcatgctcttacttcattcatgtggctctgtggtcctattgctggcatggtggtaagtaacaataccaaattatctgctctatcgatatatacgagacaaagcttttgccctcttttaaaaaaaataaatcaaattaattcatgttatggaatctgtactttctgcaatgtaaattcaagttactttagtgtcttcactacaacaaaaacttgggtatcctgaactcctgtatctatcagagtttgaagctgcattttctgtagcttctgatatgtgtaatgtagatcatactgtcataattcataagctttcataaaaaatgtttgtgtacaagtcatattttttttaattcttgatccattatcatctcccaatcctgcaggttcaaccatgcgtgggtctatacagtgataggtgcacctcaaaatggggaagacgaaggccatatattcttacaggatgtgtgttgatatgcttggctgtaagaaccactgaaccttggaactcttattccttaagttgaagtccccaaattttctctcatattgacattgcactttatgtatgtaggtcgtagttattggattttcagctgacattggatacgccatgggtgatacaaaggaagactgcaggtaactgaacattttgcgtatcataattagcagataacagttatctgatacaccatgtaatgcagtgtctaccatggatcacggtggcatgctgctattgtatatgtcctgggattctggcttcttgatttttccaacaacactgtgcaggttagtgcctactatataataatcatgttaaagaaatttgtacaaagaatcataacaaaaatcttaattttgcaatgcaatttctagggtccagcacgtgctctaatggctgatttatcaggtaatttttcatagccttgtctttcagagttaactaccaaatttttattggtttccacagttcactaacacttgattgattccagaaaaaaaaaagttgcccttgtgttctgggcttctggtatcagtgggattttggatttgatctcctaattttcgttctgtaggacgtcatggacctggcacagctaactccatcttctgttcttggatggcaatgggaaacatcctaggatactcctctggttccacaaacaattggcacaagtaagttactcttatgtatcagtttcatacagattttctgtttagcacagtctattcagttgcattcttttctgaaagaaggcaagtgaaattcatattacattagtagagaaggtgaaatttgtttacaagtattgagttgcattctccctaattaattgcgcaggtggtttccattccttaaaacaagagcttgctgtgaagcttgtgcaaatttgaaaggtgcatttctggtggctgtggtaagttctgttattacctcataaactgcctgctgataatactttaacaatgtgctaatattagtctttgtaataagatagtactatactgaaaatattttagcgagtatgagtaatttaacttacatattgtattgctgttcctcttttttcaaccctgtcatattggttgctttttttcacagcctaacatgctcttgtttggtcattttcccctgttttcagattttcctgtccctgtgcttggttataactctgatatttgccaaggaagttccattcaaaggaaatgcagccctcccaacaaaatcaaacgagccagctgagcctgaaggcactggtccactagcagtgttgaaaggcttcaggaacttacctactgggatgccttctgtgcttattgtgactggccttacctgggtatgcaatatacatcagaaaaagaacttttcagattattgaaaaaattgctactagatcatatcttagttatatcttatagtaccttaccttttctatgatatgattgtccttgtagttgtcttggttcccattcatcctctacgacactgactggatgggccgtgagatctaccacggtgacccaaagggaactgatcctcagatcgaggctttcaaccagggtgtcagggcaggtgcatttggcctgctactgaattcggtaagatctgattggtttctgaattctgattgccacaactactgtcaaatctttaacactcaagccatgttctttggcagcaactaaccatgggcttctgatcgtgcagattgtgctaggattcagctcattcttgattgaaccaatgtgccggaaagtcgggccaagggtggtgtgggtgacgagcaatttcctcgtctgcatcgccatggctgcaactgcactgatcagcttctggtcactcaaggacttccatggaactgttcagaaagccatcactgcagacaagagcatcaaggctgtctgcctcgtcctctttgcgtttcttggagtccctctcgccgtaagaatcactgacacttcaacttaaacacattgctaaaaaaaattatttcctcaagaatgccttatcctgatgaactgtctgaaaatttgcgcgatgcaggttctgtacagtgttcccttcgcagtgacggcgcaattagcggccactagagggggtggccaaggtatgaatcccaacattcttgcagcttttcttgacataattcggtgttgtttggatgctggtctaatccatggcgatctgcagggctctgcaccggcgtcctcaacatctccatcgtcatccctcaggttaattagctgccaaattctaatccgtttcccttgaaaccgaatcagatatacttcatggattattagaatttgaatgctcatggattcatggttgcgtggtgttcttccctcaggtggtcatcgcgctgggagccgggccgtgggacgagctgttcgggaaggggaacatcccggcgttcggcctcgcctcgggattcgcgctcatcggcggcgtcgccggaatattcctgctgcccaagatctccaagcgccagttccggtcggtcagcatgggcggcggtcactgaccgccccgctctcgccgccggctgcggcggcggcggcggccggagcagcgccgccggaagtggtgagctttgtagttctcgtttgcaatggatcaaaggagccccctctttttttttacatagggtaattaattaattaaggctgttaattaggaaggggtgaggtggccctgcagctggggcccacgctttgttgccacctcggtagaagagaataagcagatcatgcagataatatggaactg</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055646.1 RefSeq:Os03g0170900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

Os03g0170900

2014-06-10T02:56:16Z

Sean: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.
It's the first sucrose transporter gene in rice, rice sucrose transport gene, named ''OsSUT1''. It might not play an important role in carbon metabolism of rice, but participate in the long distance transportation of assimilation product, and plays an important role in the process of sucrose by apoplast pathway.
The expression changes of ''OsSUT1'' gene adjusted salt stress response of the Taipei 309 rice varieties (Siahpoosh et al. 2012).

OsSUT1 didn’t directly involve in sucrose transport between epithelial cells in seed scutellum or between aleurone cells during the process of germination. However, in the companion cells and keratin vascular bundle, OsSUT1 may participate in the sucrose loading of phloems to transport to the developing buds and roots. OsSUT1 may also participate in regaining sucrose from the phloem tissue of taproots, coleoptiles and first and second leaves and other parts (Scofield et al., 2007).

Rice sucrose transporters OsSUT1, participate in transport of assimilation product over long distances. OsSUT1 function has been previously tested in the germination and early seedling growth. In this article, the author tested the role of OsSUT1 in assimilate during the whole transport over long distances (from flag blades to the base of the grouting seeds). GUS expression drived by OsSUT1 promoter and immune positioning with OsSUT1 shows that, in the process of grouting, GUS activity and OsSUT1 protein appeared in the mature phloem organized of all the nutrients involved in the assimilation product long-distance transportation way. In addition, OsSUT1 is detected expressing in flag foliage and leaf sheath before earing. GUS activity mainly appeared in the phloem companion cell cell, while its protein in the companion cell and molecular sieve tube. RT-PCR detection showed OsSUT1 transcriptions expressed in the toppest internode (internode 1). The location datas of OsSUT1 is concerned with the starch and sucrose content in these organizations and carbohydrates stored in the stems. In order to detect the connection between the cells, we proceeded the feeding dyeing experiments. The results showed that the symplast between blades of flag leaves, leaf sheath and internode 1 phloem have continuity with parenchyma cells around. Therefore, it’s believed that, during the process of sucrose transport, OsSUT1 may participate in recycling spilled sucrose (Scofield et al., 2007).

Destruction of rice sucrose transporters OsSUT1 damage the function of pollen but does not affect the mature of pollen. As is known to all, in all kinds of sucrose transport steps, sucrose transporters (SUTs) plays an important role in the process of sucrose by apoplast pathway. Due to the developing pollen is symplast separated withanther tissue, SUTs may play a role in the process of sucrose by apoplast pathway. In order to verify this possibility, we anlyze SUT gene family in pubertal pollen of rice by RT-PCR and laser micro-dissection. In five SUT genes, ''OsSUT1'' and ''OsSUT3'' expressed in pollen, while the expression of both have time specificity and are not identical. ''OsSUT1'' are expressed in pollen, determined by using ''OsSUT1'' promoter drive GUS expression. In order to further study ''OsSUT1'' function, we found insertion mutants.Tthe homozygous mutants (''SUT1'' - / -) can’t be obtained Heterozygous mutants (''SUT1'' + / -) have grain with normal grouting. However, the lose of ''OsSUT1'' does not affect the starch accumulation in the process of pollen development, suggesting that there may be other sucrose transporters involved in starch biosynthesis (Hirose et al., 2010).

Five sucrose transport gene of rice are expressed in xenopus oocyte and determined by the method of electrophysiology for their activities. It showed that OsSUT1 and OsSUT5 can generate sucrose-dependent electricity through the two electrode voltage clamp technique analysis. We tested the dynamic character of sucrose transport, substrate specificity, PH dependency and sucrose K0.5. OsSUT1 show the similar characteristics with ⅡSUT of all monocotyledon detected before: K0.5 is 7.50 mM, the optimal pH5.6. Compared with OsSUT1, substrate affinity of OsSUT5 is slightly higher (K0.5 is 2.32 mM, the optimal pH5.6). When compared with all Ⅱ SUTs before, substrate specificity of OsSUT5 is a little poorer, pH dependence lower. Meanwhile, the authour detected the regulation of reduced glutathione GSH and oxidized glutathione GSSG for each OsSUTs and ZmSUT1 in corn and HvSUT1 in barley, and found GSH and GSSG had no significant effect on these SUTs activity in xenopus oocyte. In conclusion, transport active differences between OsSUT1 and OsSUT5 has certain scope, which may be adapted to their functions in plants (Sun et al., 2010)

===Expression===
Please input expression information here.
''OsSUT1'' is expressed highly in the germination of the seed source and ears, lowly in roots (Aoki et al., 2003).

GUS expression drived by OsSUT1 promoter and immune positioning with OsSUT1 shows that, in the process of grouting, GUS activity and OsSUT1 protein appeared in the mature phloem organized of all the nutrients involved in the assimilation product long-distance transportation way. In addition, OsSUT1 is detected expressing in flag foliage and leaf sheath before earing. GUS activity mainly appeared in the phloem companion cell cell, while its protein in the companion cell and molecular sieve tube. （Scofield et al., 2007）

In five SUT genes, ''OsSUT1'' and ''OsSUT3'' expressed in pollen, while the expression of both have time specificity and are not identical. ''OsSUT1'' are expressed in pollen, determined by using ''OsSUT1'' promoter drive GUS expression. （Hirose et al., 2010）

===Evolution===
Please input evolution information here.
Using ''OsSUT1'' and ''HvSUT2'' blast rice genome and get four SUTs, ''OsSUT2'', ''OsSUT3'', ''OsSUT4'' and ''OsSUT5''. These four genes and ''OsSUT1'' constitutes the sucrose transporters of rice gene families. The structure prediction of proteins translated from five SUT genes show that all have 12 transmembrane helical and a domain structure highly conserved in all known SUTs, suggesting the four ''SUTs'' coding functional sucrose transporters.

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==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0170900|
Description = Sucrose transporter|
Version = NM_001055646.1 GI:115451020 GeneID:4331775|
Length = 6326 bp|
Definition = Oryza sativa Japonica Group Os03g0170900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:3776143..3782468|
CDS = 3776388..3776561,3776678..3776721,3776805..3776868,3776971..3777204,3777314..3777457 ,3777576..3777770,3777996..3778071,3778238..3778337,3778504..3778537 ,3778625..3778709,3778775..3778839,3778929..3779027,3779359..3779424 ,3782034..3782270|
GCID = <gbrowseImage1>
name=NC_008396:3776143..3782468
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:3776143..3782468
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctcgcggcagcggggccggaggaggcggcggcggtggtggcggggggctggagctgtcggtcggggtcggcggcggcggcggcgcgcgtggtgggggaggcggtgaggcggcggcggcggtggagacggcggcgccgatcagcctcgggaggctcatcctctccgggatggtcgccggcggcgtgcagtacgggtgggcgctccagctctccctcctcaccccctacgtccagacactcggactgtcgcatgctcttacttcattcatgtggctctgtggtcctattgctggcatggtggttcaaccatgcgtgggtctatacagtgataggtgcacctcaaaatggggaagacgaaggccatatattcttacaggatgtgtgttgatatgcttggctgtcgtagttattggattttcagctgacattggatacgccatgggtgatacaaaggaagactgcagtgtctaccatggatcacggtggcatgctgctattgtatatgtcctgggattctggcttcttgatttttccaacaacactgtgcagggtccagcacgtgctctaatggctgatttatcaggacgtcatggacctggcacagctaactccatcttctgttcttggatggcaatgggaaacatcctaggatactcctctggttccacaaacaattggcacaagtggtttccattccttaaaacaagagcttgctgtgaagcttgtgcaaatttgaaaggtgcatttctggtggctgtgattttcctgtccctgtgcttggttataactctgatatttgccaaggaagttccattcaaaggaaatgcagccctcccaacaaaatcaaacgagccagctgagcctgaaggcactggtccactagcagtgttgaaaggcttcaggaacttacctactgggatgccttctgtgcttattgtgactggccttacctggttgtcttggttcccattcatcctctacgacactgactggatgggccgtgagatctaccacggtgacccaaagggaactgatcctcagatcgaggctttcaaccagggtgtcagggcaggtgcatttggcctgctactgaattcgattgtgctaggattcagctcattcttgattgaaccaatgtgccggaaagtcgggccaagggtggtgtgggtgacgagcaatttcctcgtctgcatcgccatggctgcaactgcactgatcagcttctggtcactcaaggacttccatggaactgttcagaaagccatcactgcagacaagagcatcaaggctgtctgcctcgtcctctttgcgtttcttggagtccctctcgccgttctgtacagtgttcccttcgcagtgacggcgcaattagcggccactagagggggtggccaagggctctgcaccggcgtcctcaacatctccatcgtcatccctcaggtggtcatcgcgctgggagccgggccgtgggacgagctgttcgggaaggggaacatcccggcgttcggcctcgcctcgggattcgcgctcatcggcggcgtcgccggaatattcctgctgcccaagatctccaagcgccagttccggtcggtcagcatgggcggcggtcactga</cdnaseq>|
AA = <aaseq>MARGSGAGGGGGGGGGGLELSVGVGGGGGARGGGGGEAAAAVET AAPISLGRLILSGMVAGGVQYGWALQLSLLTPYVQTLGLSHALTSFMWLCGPIAGMVV QPCVGLYSDRCTSKWGRRRPYILTGCVLICLAVVVIGFSADIGYAMGDTKEDCSVYHG SRWHAAIVYVLGFWLLDFSNNTVQGPARALMADLSGRHGPGTANSIFCSWMAMGNILG YSSGSTNNWHKWFPFLKTRACCEACANLKGAFLVAVIFLSLCLVITLIFAKEVPFKGN AALPTKSNEPAEPEGTGPLAVLKGFRNLPTGMPSVLIVTGLTWLSWFPFILYDTDWMG REIYHGDPKGTDPQIEAFNQGVRAGAFGLLLNSIVLGFSSFLIEPMCRKVGPRVVWVT SNFLVCIAMAATALISFWSLKDFHGTVQKAITADKSIKAVCLVLFAFLGVPLAVLYSV PFAVTAQLAATRGGGQGLCTGVLNISIVIPQVVIALGAGPWDELFGKGNIPAFGLASG FALIGGVAGIFLLPKISKRQFRSVSMGGGH</aaseq>|
DNA = <dnaseqindica>5908..6081#5748..5791#5601..5664#5265..5498#5012..5155#4699..4893#4398..4473#4132..4231#3932..3965#3760..3844#3630..3694#3442..3540#3045..3110#199..435#aaacacaaacccaccacctcctcctcctcctcccatccagcacgcgcctcctctctcgcgcggctttccatttccatctccccctcctcctcctacgtctccgccgctcctcacttcctccactcgatttcctttcttggcctctcctcctctgacacaggggtgtgcaggtttgtgtttgtgcgtggcgcgtccgccatggctcgcggcagcggggccggaggaggcggcggcggtggtggcggggggctggagctgtcggtcggggtcggcggcggcggcggcgcgcgtggtgggggaggcggtgaggcggcggcggcggtggagacggcggcgccgatcagcctcgggaggctcatcctctccgggatggtcgccggcggcgtgcagtacgggtgggcgctccagctctccctcctcaccccctacgtccaggtatatatatatatatactgtactcctgtgtattttgcacactctcatgcatgcatctgaaactctgaataatcctgcaaaaaatatatacaagtatctcacgtgtggatttgattttgttcgcgagtttttgctcgtgctagctgttctaatctgcaaggtttcatggtagtagattatatatgtcctggttacaatcttttcaaggttataatcttgtaatttttttctgctttgtcaatatttacttcgcactgtctggtgtgagtcgttaaaaaaatatatatcttgaaagattggggaatttttcttcatgtgtggggagagatttatttttttttgtttgaacggtagatttgactagtgtctaaaagtaccgtaataaattcaatctgcatgccttttgtaattttttttgctcatgtgtttatatacatgtggccaaatatgcatttgatgcactagctatcatttttgtcattttgtagttattccttttcctttcctttttttttcctttttctttttgttgtcactagaggattcatatcctctcgattcctctatgaaacgtcggcgttttctgcctcttttctgaagaatctgtatacctatataagaatcaaaatcttttataagttctttttgtgcactttggatcaatcaggctagccagcatgacacttagcatcagtaccggtctactgtaccgagattcactttacgcttgacacgtttctcaactccttgtttgattccattttacctcaattagactgtttgattccgttttatctcagatatgacactcctttttttaaaattccattttacctcagatgctgtacagtgccataaaaaaatatacatctgctccaactcaccttatccttcttgtaataaccacgtattaggttaatagctactaatagctagagatgtaaacaaaaataagcagacaagggcaaaagtgagcggtaataatggagaattaatctgtcgttcctccgcgcgcagttaaaggtaggcagatgacgggtccctcccctctattccatactaaccgcaagatgaaactaatattcttactgctagatatataaatcatccttcatttagcttcatctccctccttaagatgtgttcttttcttccatttcctaacttctactcccttcgtttgacgtacacatatttcctaaactgccaaacgatgtgtttctactaaaaatttatatggtttaaaaaacatattaatatatttttttttgtctttttagctaatatttaattaattatgtgctaaactaccgctcaattttgtgtaccgggagaaagggttctcaatcattacaaaagaacacagcctagtctccatgaaaaacatcacttttattttaagacagagcaagtggagcgattttataactttactatagtacaaaattaatggtcagaaatttaaaattttgaccgaattttatcgtaagcatcaaatatttattaccggagagatagagtattggttatttgcctctctgtcgtcaaatattctaaacaaagacgatcttcttagtggttaaacttaaatctcagtccttccaggcgtttatttgaaggatttatagcggagataaacagctccttttttttcggaaatacgtaaggagggcacttttgtattaaggggaataaaaagggtctgtacagggaaaaccaaaaaacagcaggggacaggaataaacagctcctcacttcctctttgtatcagtccatactaattaacaaagtgttccaaaatgttctgtactttttctgtagaacattatatgcagttcatcacccctgatggtccatgcatcaggtctagctacctactgttgtattgaagtatttctggcgaagtttatccttaccttagccttctcgagccaagtgccataaagatggttgctgtagaggcaactccaacccactttaaccgcgcacatgaaaaataaaggggtaaaacaaggaaattctaggtcagatatatatgtcctgtggactgtggcacagttgcaacagagcaattgcataggcaaatgagggcagatcatcatgtacccatagcctgttggtgctatgttagcatccaagcattgccattttaactattaagattcttcttcttatatttgacaagtgaaataaatttagtgactgaacatatatagaacgatgaacagtctttgtatgtttattcatgccatgggggaaagagaagtgtgatctgaagttattggatagatagctgttagttgtagcatgcaaggcaatgcatcctggaaccaacagaccaacagagctggggatatttcatctgctaaatttctgggaaaatttattgtgtggtaatcaatatgtgttcactctttgcaactggtacacgctctctgatgtttctgaatatcttccacagtgatgcagatcttgttgacacagcaaactcaacaaacatagtcatttttttaaggaatgaagcatgatcatctattgtcagagtagattacagtcaaagtttttttataagccaaaacaactgattttctaacctctgttatttacagacactcggactgtcgcatgctcttacttcattcatgtggctctgtggtcctattgctggcatggtggtaagtaacaataccaaattatctgctctatcgatatatacgagacaaagcttttgccctcttttaaaaaaaataaatcaaattaattcatgttatggaatctgtactttctgcaatgtaaattcaagttactttagtgtcttcactacaacaaaaacttgggtatcctgaactcctgtatctatcagagtttgaagctgcattttctgtagcttctgatatgtgtaatgtagatcatactgtcataattcataagctttcataaaaaatgtttgtgtacaagtcatattttttttaattcttgatccattatcatctcccaatcctgcaggttcaaccatgcgtgggtctatacagtgataggtgcacctcaaaatggggaagacgaaggccatatattcttacaggatgtgtgttgatatgcttggctgtaagaaccactgaaccttggaactcttattccttaagttgaagtccccaaattttctctcatattgacattgcactttatgtatgtaggtcgtagttattggattttcagctgacattggatacgccatgggtgatacaaaggaagactgcaggtaactgaacattttgcgtatcataattagcagataacagttatctgatacaccatgtaatgcagtgtctaccatggatcacggtggcatgctgctattgtatatgtcctgggattctggcttcttgatttttccaacaacactgtgcaggttagtgcctactatataataatcatgttaaagaaatttgtacaaagaatcataacaaaaatcttaattttgcaatgcaatttctagggtccagcacgtgctctaatggctgatttatcaggtaatttttcatagccttgtctttcagagttaactaccaaatttttattggtttccacagttcactaacacttgattgattccagaaaaaaaaaagttgcccttgtgttctgggcttctggtatcagtgggattttggatttgatctcctaattttcgttctgtaggacgtcatggacctggcacagctaactccatcttctgttcttggatggcaatgggaaacatcctaggatactcctctggttccacaaacaattggcacaagtaagttactcttatgtatcagtttcatacagattttctgtttagcacagtctattcagttgcattcttttctgaaagaaggcaagtgaaattcatattacattagtagagaaggtgaaatttgtttacaagtattgagttgcattctccctaattaattgcgcaggtggtttccattccttaaaacaagagcttgctgtgaagcttgtgcaaatttgaaaggtgcatttctggtggctgtggtaagttctgttattacctcataaactgcctgctgataatactttaacaatgtgctaatattagtctttgtaataagatagtactatactgaaaatattttagcgagtatgagtaatttaacttacatattgtattgctgttcctcttttttcaaccctgtcatattggttgctttttttcacagcctaacatgctcttgtttggtcattttcccctgttttcagattttcctgtccctgtgcttggttataactctgatatttgccaaggaagttccattcaaaggaaatgcagccctcccaacaaaatcaaacgagccagctgagcctgaaggcactggtccactagcagtgttgaaaggcttcaggaacttacctactgggatgccttctgtgcttattgtgactggccttacctgggtatgcaatatacatcagaaaaagaacttttcagattattgaaaaaattgctactagatcatatcttagttatatcttatagtaccttaccttttctatgatatgattgtccttgtagttgtcttggttcccattcatcctctacgacactgactggatgggccgtgagatctaccacggtgacccaaagggaactgatcctcagatcgaggctttcaaccagggtgtcagggcaggtgcatttggcctgctactgaattcggtaagatctgattggtttctgaattctgattgccacaactactgtcaaatctttaacactcaagccatgttctttggcagcaactaaccatgggcttctgatcgtgcagattgtgctaggattcagctcattcttgattgaaccaatgtgccggaaagtcgggccaagggtggtgtgggtgacgagcaatttcctcgtctgcatcgccatggctgcaactgcactgatcagcttctggtcactcaaggacttccatggaactgttcagaaagccatcactgcagacaagagcatcaaggctgtctgcctcgtcctctttgcgtttcttggagtccctctcgccgtaagaatcactgacacttcaacttaaacacattgctaaaaaaaattatttcctcaagaatgccttatcctgatgaactgtctgaaaatttgcgcgatgcaggttctgtacagtgttcccttcgcagtgacggcgcaattagcggccactagagggggtggccaaggtatgaatcccaacattcttgcagcttttcttgacataattcggtgttgtttggatgctggtctaatccatggcgatctgcagggctctgcaccggcgtcctcaacatctccatcgtcatccctcaggttaattagctgccaaattctaatccgtttcccttgaaaccgaatcagatatacttcatggattattagaatttgaatgctcatggattcatggttgcgtggtgttcttccctcaggtggtcatcgcgctgggagccgggccgtgggacgagctgttcgggaaggggaacatcccggcgttcggcctcgcctcgggattcgcgctcatcggcggcgtcgccggaatattcctgctgcccaagatctccaagcgccagttccggtcggtcagcatgggcggcggtcactgaccgccccgctctcgccgccggctgcggcggcggcggcggccggagcagcgccgccggaagtggtgagctttgtagttctcgtttgcaatggatcaaaggagccccctctttttttttacatagggtaattaattaattaaggctgttaattaggaaggggtgaggtggccctgcagctggggcccacgctttgttgccacctcggtagaagagaataagcagatcatgcagataatatggaactg</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055646.1 RefSeq:Os03g0170900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]