http://192.168.164.12:81/ricewiki/api.php?action=feedcontributions&feedformat=atom&user=Shixing 2026-08-27T11:29:56Z User contributions MediaWiki 1.30.0 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0586200&diff=169580 2014-05-19T01:27:07Z

<p>Shixing: Undo revision 169579 by Shixing (talk)</p> <hr /> <div>''OsJAR1'',one kind of Jasmonoyl-isoleucine (JA-Ile) synthase, belongs to class I ''GH3'' protein family in rice,and it is required for the response to wounding.<br /> ==Annotated Information==<br /> ===Function===<br /> [[File:Expression.figure1.png|thumb|right|'''Figure1:'''''Gene expression and hormone content in wild type and OsJAR1 TOS17 insertion lines after simulated herbivory (WOS)(frome reference&lt;ref name=ref1/&gt;).'']]<br /> [[File:Expression.figure2.png|thumb|right|'''Figure2:'''''The expression levels of OsJAR1 and OsJAR2 mRNA and the accumulation of JA and JA-amino acid conjugates in response to wounding(from reference&lt;ref name=ref2/&gt;).'']]<br /> ''OsJAR1'' contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine(JA-Ile) involved in defense responses in rice by the use of an osjar1 Tos17 mutant.&lt;ref name=ref4/&gt;It is involved in phytochrome and jasmonate signalling. In far-red and blue lights, osjar1 coleoptiles were longer if compared with the wild type (WT), indicating that ''OsJAR1'' participates in the suppression of coleoptile elongation in these light conditions, while the mutant did not show a clear phenotype in red light.''OsJar1'' modulates light and JA signalling in the photomorphogenesis of rice.&lt;ref name=ref3/&gt; Light can induce jasmonate-isoleucine conjugation via OsJAR1-dependent pathways in rice. ''OsJAR1'' is the most important JA-Ile conjugating enzyme in the wounding response during the vegetative stage.&lt;ref name=ref5/&gt;Plants produce jasmonic acid (JA) and its amino acid conjugate, jasmonoyl‐L‐isoleucine (JA‐Ile) as major defense signals in response to wounding and herbivory. OsJAR1‐deficient plants have strongly reduced JA‐Ile levels after WOS&lt;ref name=ref1/&gt;.<br /> <br /> ===Expression===<br /> <br /> (1)''OsJar1'' transcripts were up-regulated transiently in response to treatment with exogenous methyl-jasmonic acid (MeJA)&lt;ref name=ref3/&gt;.The expression levels of ''OsJAR1'' and ''OsJAR2'' were induced in response to wounding with the concomitant accumulation of JA-Ile.&lt;ref name=ref2/&gt;<br /> <br /> (2)Real-time PCR was conducted according to the QuantiTect SYBR Green PCR handbook (Qiagen). PCR was perfomed in ABI PRISM 7000 cycler (PE Applied Biosystems, Warrington, UK) using QuantiTect SYBR green-mastermix with HotStar Taq DNA polymerase following the recommended conditions [15 min 95 °C, 40× (15 s, 94 °C; 30 s, 60 °C; 30 s, 72 °C)]. JAR1F2 and JAR1R2 were used as primers to amplify OsJar1 cDNA, Rubg237F and Rubg304R for ubiquitin, which was used as the endogenous control gene.<br /> <br /> For RT-PCR, first-strand cDNA synthesis kit for RT-PCR (Roche, Mannheim, Germany) was performed according to the manufacturer's description. A 1 µL aliquot of a 1:10 dilution of the RT product was used as a template in the subsequent PCR reaction. For amplification of OsJAR1 cDNA, the primers JAR1F1 and JAR1R2 were used, for ubiquitin Rubg237F and Rubg 304R. The PCR conditions were 1 min 94 °C, 30× (94 °C, 1 min; 60 °C, 1 min; 72 °C, 2 min), 72 °C, 7 min.&lt;ref name=ref3/&gt;<br /> <br /> Primers used in real-time PCR and RT-PCR:<br /> <br /> JAR1F1: TAAGGCATTACGCAGGGAAC<br /> <br /> JAR1F2: AGTGCTGAGCATCAACATCG<br /> <br /> JAR1R1: CCAGATAACAGCACCAGAAGC<br /> <br /> JAR1R2: CACCACCAAGGCTTAGGAAA<br /> <br /> Rubg237F: GAGCCTCTGTTCGTCAAGTA<br /> <br /> Rubg304R: ACTCGATGGTCCATTAAACC&lt;ref name=ref3/&gt;<br /> <br /> <br /> ===Evolution===<br /> ''OsJar1'' belongs to the ''GH3'' family in rice. Another member of this family in Arabidopsis, ''DWARF IN LIGHT 2'' (DFL2), showed 47% identities. ''Dfl2'' was shown to be a light-dependent gene, but its gene product did not show enzymatic activity on plant hormones, presumably (Takase et al. 2003).'' OsJAR1'' contained three domains described to be important for the enzymatic function of ''GH3'' proteins (Chang, Xiang &amp; Dunaway-Mariano 1997; Staswick et al. 2002; Terol et al. 2006).&lt;ref name=ref3/&gt;<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> *Biotechnology Research Center, The University of Tokyo, Tokyo 113-8657, Japan.<br /> *Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan<br /> *National Agricultural Research Center of Hokkaido region, National Agriculture and Food Research Organization, Sapporo 062-8555, Japan <br /> *Botanical Institute, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany<br /> *Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena,Germany<br /> *Department of Plant Physiology, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan<br /> *RIKEN Plant Science Center, Tsurumi,Yokohama, Kanagawa 230-0045, Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> * &lt;ref name=&quot;ref1&quot;&gt;<br /> Kaori Fukumoto, Kabir Md Alamgir, Yuko Yamashita, Ivan Galis, et al. (2013) Response of rice to insect elicitors and the role of OsJAR1 in wound and herbivory-induced JA-Ile accumulation[J]. Integr Plant Biol 55: 775–784.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref2&quot;&gt;<br /> Shinji Wakutaa, Erika Suzukia, Wataru Saburia,*, Hideyuki Matsuuraa, Kensuke Nabetaa, Ryozo Imaib and Hirokazu Matsuia (2011) OsJAR1 and OsJAR2 are jasmonyl-L-isoleucine synthases involved in wound- and pathogen-induced jasmonic acid signaling[J].Biochemical and Biophysical Research Communications,409(4): 634-639.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref3&quot;&gt;<br /> MAREN RIEMANN, MICHAEL RIEMANN and MAKOTO TAKANO. (2008) Rice JASMONATE RESISTANT 1 is involved in phytochrome and jasmonate signalling. Plant, Cell &amp; Environment 31(6):783–792.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref4&quot;&gt;<br /> Shimizu T, Miyamoto K, Miyamoto K, Minami E, Nishizawa Y, Iino M, Nojiri H, Yamane H, Okada K. (2013) OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice[J].Bioscience, Biotechnology, and Biochemistry 77(7):1556-1564.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref5&quot;&gt;<br /> KATHARINA SVYATYNA, YUSUKE JIKUMARU, RITA BRENDEL, MICHAEL REICHELT, AXEL MITHÖFER, MAKOTO TAKANO, YUJI KAMIYA, PETER NICK &amp; MICHAEL RIEMANN. (2013) Light induces jasmonate-isoleucine conjugation via OsJAR1-dependent and -independent pathways in rice.Plant, Cell &amp; Environment 37(4):827-839.<br /> &lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0586200|<br /> Description = GH3 auxin-responsive promoter family protein|<br /> Version = NM_001062995.1 GI:115465720 GeneID:4339756|<br /> Length = 5516 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0586200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:29281780..29287295|<br /> CDS = 29284407..29284687,29284793..29284894,29285094..29285911,29286499..29287043|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccgtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MTICSCEETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNF GLGGRTDAESYKSCIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKP KFIPFNDELLETTLQIYRTSYAFRNREYPIGQGKALQFVYGSKQVITKGGILATTATT NLYRRQRYKEGMKDIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAH SLVHAFQTFEEVWEDLCTDIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIG LSNWYGVIPALWPNAKYVYGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNI DPTVPPEQVTYAVLPQVGYFEFIPLEKPIGEETENSASIHYIESDPVGLTEVEVGKIY EVVITNFAGLYRYRLGDVVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVE EASKFLEGEKLEVMDFTSFVERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDA GYTGSRKIKTIGPLELRILRKGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQIL SRNVTQSYFSTAYGF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2628..2908#3014..3115#3315..4132#4720..5264#acccgctctctcctctcatctcctcctcctgtgttcgtgttcttcttcttcttgtagcagcgcttaaccaacccatcattagcgaccaaacctctctccctctccccctctgactaatcaaaattaaacaatttcgatctccaccttcctcttcttgattccagttccaccagagagagacagggtcttctctaatccatgtggtgcttgttggtctcgcttgcttcgtcttcgtcttcgtcttcctcttctcgcacctagtcccgcctcgtcgcctcctccggagaccggagccggacgggacggttgccggcgagcggcggtcgctttcttctctcggccctactcacggtcagtgattggctcccttccttcctggtgttctattgatcctttgtgggggaaaaaaggcttcgatttggggggcatgttcttgcctttttcctgttttgggggagacgagacggatttcaatatatttttttaatctggggggattatgtgtggaaaagattgttcctcgtttatctgggtagtatttggagggtcctatggggatattctttgtaaagctgggatttttaggtggtaaaataatgttgatatactttcagaaagaatatactgcagaaagtttttcagagataacagataagattaggccgtccttaatctcctttctttgcgtctgttccctccaactaggatggaaaagaatggcggattgattagtcagaaactggctgctgaatactctgagatttcatttcatttcagcctctgttgattcctcatggctgtctctaacttctttgcttgtgcttccgatttctttggcggtaaatagcctccacgaatctctggtcaacgaggtattaaaacttaaaagggcttttgtctcaggttgacccgagccatgattcgcccccctcccatgctttgcaaacaagatatgatacgcagtgctggtacaattcagagcatatgttctttcctcttttgctttgtttcagacagcaacttttagcagccacgcctctcacaagttgataaaaaaaaattttgtgttgctctcctactactcatcactttctagtgcgatatagtagtagtagtagtaggagtatatgctagcactatccacagtttagcacacagattggtggcacctgggcagatataaaagcttcacatttttatcaatgtctgcgcagttatattgtcgatttgattccttttatagatattactaggtcaagtcgtgtcgcaattactccccccctttcctccattgtagggtgttcctgtttagtccgcgggggatgctgctccctgagcctgtgtgtgtctcttgatttttctttctttgctcgcacgcagcagcagcagcatgttttgtgtcggtgttaaggaccctacataaataattcatcaaatcaagtatcttttgtctgttttggggaatctcctttgcggttcgtccaaaatttcgcgaaagaaacaattctatatgactgcttttgtcgagaggttagacatcactgctagagtagtagagcaatgctacacgtactaaatttttcttactaaacttttactaataatcatctcaaccgtttgatttaagtagatggaatttacaaaaaatctagatgcactcgtagcatgacacatcagtgttagtaagaatttagtaagaaaaagttagtacgtatagccttttccagagtagtactagcagagtgttagattgtatcacctgctcgggcagttgtaaatggtatggtgagttggtgactggtttttacagttttttcatacttgggtttatggaggatttactgttatggagacctgtaaaaaatgttacatatattgctactttctcatttggatttcggatggctgttttttttatcattatgttggtaaagttttgcatcaagaatctgaaccagcttagcaatactaatagtagatggttcagtaagctgcttccatgtccttgcaactttgcaatgctggcaattttttttcgaaaaagagcaaggctggcaatatgtgtatagcttttgtttggcaatatatgttgcgattctagcgctggtggtatctcaagatcagaaaaagtgagcatcaatgtcttactttattggttttctgactgattacattagtaaacgctgtgtccaaaagtatagagtaatactagttatattttcttaattgtcagtgactcagtatccgcagatggtttgtaaagtgcccttatttttcatggtcgttagctttagtaggtatatagtgtactcttgatggtttatcctttttttcgcccctttctcctttgtcatctgggcgcacgttgctatgcagtttcatgagatggttagtgcgtatccaactattctgtcggcaataaagttccacaatcttcaaaattttctaccattctgatgccaactcctactgcactatcagctcttggtttccagctcagttagtggttagaaattgcagcctacatgaaaaagtaactaatagattttctcgtctccaccttttatagttattttcagtgactcttcttccaaaccaagtggtaaagccaggattctaccattacaatgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcaggtactgtacccattgcttgaaatttgtcaccttattatgcttatcgtttactagtttatgataataaatgttcatagtctggcttcttctgtggctttcatacagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccggtaaggatctctgatttattattatttatgctcaagtaatgctgctttttctgaatgttcaacaaactttgatgatcaggttcaacatgagttattgtttttatacactgttttgcatgctattaacttgttgcttctttgtgtattttttactctgcatcccagaatattagaataaccttctgttttgtgtttacagtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtatattctctcttttctatctattctgaatcttaaattgggttaaatctccattatctagaagtatagcaatgaaaattctacaaaaagcaaggggcagccatccctagttctgcttctggtgctgttatctggtcagacatgataccaacgggatttatcagaaataactagataagtttgtgtgacatattatttcaaaaaagaaatatttattagtcttgccccttcaatcttaaacctctttttgacaagagctccttattatgtaaaaaacataaaaatcacacggttgcaatgtatacatcaatattattaagcacatgggggagtaattattgtgaatgtttgtggtccactccttgaactctttgctgcttactcattatgattatcttaaaactctatcagatgatatatgtttctttttgtgtatgccaaccatgccagactcaccaataacatttttattctttaatatctctggctaacatacaattattttgttttaggacacagaaagtttacttattacggtgatgtttaagaaacactaaaatctttttcttttttctattttgttctaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttgatacaaagaaacatattagatgctagcagctgcatgtaattacctttttacctcttgtttttcagctttactgtagtctccaccggaagaaaacgtttggtttagtctcttgaataatctattttcttatcctgtttgtgactatctagaatctatgtaaatggcccagctctagtgaaagttatggtgatgttatcaggaaagaaaatgtggtgtttcccgtaacaagtgctgcaaagtattattcagactc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062995.1 RefSeq:Os05g0586200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0586200&diff=169579 2014-05-19T01:26:03Z

<p>Shixing: /* Annotated Information */</p> <hr /> <div>''OsJAR1'',one kind of Jasmonoyl-isoleucine (JA-Ile) synthase,belongs to class I GH3 progein family in rice,and it is required for the response to wounding.<br /> 差评<br /> ==Annotated Information==<br /> ===Function===<br /> [[File:Expression.figure1.png|thumb|right|'''Figure1:'''''Gene expression and hormone content in wild type and OsJAR1 TOS17 insertion lines after simulated herbivory (WOS)(frome reference&lt;ref name=ref1/&gt;).'']]<br /> [[File:Expression.figure2.png|thumb|right|'''Figure2:'''''The expression levels of OsJAR1 and OsJAR2 mRNA and the accumulation of JA and JA-amino acid conjugates in response to wounding(from reference&lt;ref name=ref2/&gt;).'']]<br /> ''OsJAR1'' contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine(JA-Ile) involved in defense responses in rice by the use of an osjar1 Tos17 mutant.&lt;ref name=ref4/&gt;It is involved in phytochrome and jasmonate signalling. In far-red and blue lights, osjar1 coleoptiles were longer if compared with the wild type (WT), indicating that ''OsJAR1'' participates in the suppression of coleoptile elongation in these light conditions, while the mutant did not show a clear phenotype in red light.''OsJar1'' modulates light and JA signalling in the photomorphogenesis of rice.&lt;ref name=ref3/&gt; Light can induce jasmonate-isoleucine conjugation via OsJAR1-dependent pathways in rice. ''OsJAR1'' is the most important JA-Ile conjugating enzyme in the wounding response during the vegetative stage.&lt;ref name=ref5/&gt;Plants produce jasmonic acid (JA) and its amino acid conjugate, jasmonoyl‐L‐isoleucine (JA‐Ile) as major defense signals in response to wounding and herbivory. OsJAR1‐deficient plants have strongly reduced JA‐Ile levels after WOS&lt;ref name=ref1/&gt;.<br /> <br /> ===Expression===<br /> <br /> (1)''OsJar1'' transcripts were up-regulated transiently in response to treatment with exogenous methyl-jasmonic acid (MeJA)&lt;ref name=ref3/&gt;.The expression levels of ''OsJAR1'' and ''OsJAR2'' were induced in response to wounding with the concomitant accumulation of JA-Ile.&lt;ref name=ref2/&gt;<br /> <br /> (2)Real-time PCR was conducted according to the QuantiTect SYBR Green PCR handbook (Qiagen). PCR was perfomed in ABI PRISM 7000 cycler (PE Applied Biosystems, Warrington, UK) using QuantiTect SYBR green-mastermix with HotStar Taq DNA polymerase following the recommended conditions [15 min 95 °C, 40× (15 s, 94 °C; 30 s, 60 °C; 30 s, 72 °C)]. JAR1F2 and JAR1R2 were used as primers to amplify OsJar1 cDNA, Rubg237F and Rubg304R for ubiquitin, which was used as the endogenous control gene.<br /> <br /> For RT-PCR, first-strand cDNA synthesis kit for RT-PCR (Roche, Mannheim, Germany) was performed according to the manufacturer's description. A 1 µL aliquot of a 1:10 dilution of the RT product was used as a template in the subsequent PCR reaction. For amplification of OsJAR1 cDNA, the primers JAR1F1 and JAR1R2 were used, for ubiquitin Rubg237F and Rubg 304R. The PCR conditions were 1 min 94 °C, 30× (94 °C, 1 min; 60 °C, 1 min; 72 °C, 2 min), 72 °C, 7 min.&lt;ref name=ref3/&gt;<br /> <br /> Primers used in real-time PCR and RT-PCR:<br /> <br /> JAR1F1: TAAGGCATTACGCAGGGAAC<br /> <br /> JAR1F2: AGTGCTGAGCATCAACATCG<br /> <br /> JAR1R1: CCAGATAACAGCACCAGAAGC<br /> <br /> JAR1R2: CACCACCAAGGCTTAGGAAA<br /> <br /> Rubg237F: GAGCCTCTGTTCGTCAAGTA<br /> <br /> Rubg304R: ACTCGATGGTCCATTAAACC&lt;ref name=ref3/&gt;<br /> <br /> <br /> ===Evolution===<br /> ''OsJar1'' belongs to the ''GH3'' family in rice. Another member of this family in Arabidopsis, ''DWARF IN LIGHT 2'' (DFL2), showed 47% identities. ''Dfl2'' was shown to be a light-dependent gene, but its gene product did not show enzymatic activity on plant hormones, presumably (Takase et al. 2003).'' OsJAR1'' contained three domains described to be important for the enzymatic function of ''GH3'' proteins (Chang, Xiang &amp; Dunaway-Mariano 1997; Staswick et al. 2002; Terol et al. 2006).&lt;ref name=ref3/&gt;<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> *Biotechnology Research Center, The University of Tokyo, Tokyo 113-8657, Japan.<br /> *Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan<br /> *National Agricultural Research Center of Hokkaido region, National Agriculture and Food Research Organization, Sapporo 062-8555, Japan <br /> *Botanical Institute, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany<br /> *Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena,Germany<br /> *Department of Plant Physiology, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan<br /> *RIKEN Plant Science Center, Tsurumi,Yokohama, Kanagawa 230-0045, Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> * &lt;ref name=&quot;ref1&quot;&gt;<br /> Kaori Fukumoto, Kabir Md Alamgir, Yuko Yamashita, Ivan Galis, et al. (2013) Response of rice to insect elicitors and the role of OsJAR1 in wound and herbivory-induced JA-Ile accumulation[J]. Integr Plant Biol 55: 775–784.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref2&quot;&gt;<br /> Shinji Wakutaa, Erika Suzukia, Wataru Saburia,*, Hideyuki Matsuuraa, Kensuke Nabetaa, Ryozo Imaib and Hirokazu Matsuia (2011) OsJAR1 and OsJAR2 are jasmonyl-L-isoleucine synthases involved in wound- and pathogen-induced jasmonic acid signaling[J].Biochemical and Biophysical Research Communications,409(4): 634-639.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref3&quot;&gt;<br /> MAREN RIEMANN, MICHAEL RIEMANN and MAKOTO TAKANO. (2008) Rice JASMONATE RESISTANT 1 is involved in phytochrome and jasmonate signalling. Plant, Cell &amp; Environment 31(6):783–792.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref4&quot;&gt;<br /> Shimizu T, Miyamoto K, Miyamoto K, Minami E, Nishizawa Y, Iino M, Nojiri H, Yamane H, Okada K. (2013) OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice[J].Bioscience, Biotechnology, and Biochemistry 77(7):1556-1564.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref5&quot;&gt;<br /> KATHARINA SVYATYNA, YUSUKE JIKUMARU, RITA BRENDEL, MICHAEL REICHELT, AXEL MITHÖFER, MAKOTO TAKANO, YUJI KAMIYA, PETER NICK &amp; MICHAEL RIEMANN. (2013) Light induces jasmonate-isoleucine conjugation via OsJAR1-dependent and -independent pathways in rice.Plant, Cell &amp; Environment 37(4):827-839.<br /> &lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0586200|<br /> Description = GH3 auxin-responsive promoter family protein|<br /> Version = NM_001062995.1 GI:115465720 GeneID:4339756|<br /> Length = 5516 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0586200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:29281780..29287295|<br /> CDS = 29284407..29284687,29284793..29284894,29285094..29285911,29286499..29287043|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccgtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MTICSCEETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNF GLGGRTDAESYKSCIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKP KFIPFNDELLETTLQIYRTSYAFRNREYPIGQGKALQFVYGSKQVITKGGILATTATT NLYRRQRYKEGMKDIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAH SLVHAFQTFEEVWEDLCTDIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIG LSNWYGVIPALWPNAKYVYGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNI DPTVPPEQVTYAVLPQVGYFEFIPLEKPIGEETENSASIHYIESDPVGLTEVEVGKIY EVVITNFAGLYRYRLGDVVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVE EASKFLEGEKLEVMDFTSFVERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDA GYTGSRKIKTIGPLELRILRKGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQIL SRNVTQSYFSTAYGF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2628..2908#3014..3115#3315..4132#4720..5264#acccgctctctcctctcatctcctcctcctgtgttcgtgttcttcttcttcttgtagcagcgcttaaccaacccatcattagcgaccaaacctctctccctctccccctctgactaatcaaaattaaacaatttcgatctccaccttcctcttcttgattccagttccaccagagagagacagggtcttctctaatccatgtggtgcttgttggtctcgcttgcttcgtcttcgtcttcgtcttcctcttctcgcacctagtcccgcctcgtcgcctcctccggagaccggagccggacgggacggttgccggcgagcggcggtcgctttcttctctcggccctactcacggtcagtgattggctcccttccttcctggtgttctattgatcctttgtgggggaaaaaaggcttcgatttggggggcatgttcttgcctttttcctgttttgggggagacgagacggatttcaatatatttttttaatctggggggattatgtgtggaaaagattgttcctcgtttatctgggtagtatttggagggtcctatggggatattctttgtaaagctgggatttttaggtggtaaaataatgttgatatactttcagaaagaatatactgcagaaagtttttcagagataacagataagattaggccgtccttaatctcctttctttgcgtctgttccctccaactaggatggaaaagaatggcggattgattagtcagaaactggctgctgaatactctgagatttcatttcatttcagcctctgttgattcctcatggctgtctctaacttctttgcttgtgcttccgatttctttggcggtaaatagcctccacgaatctctggtcaacgaggtattaaaacttaaaagggcttttgtctcaggttgacccgagccatgattcgcccccctcccatgctttgcaaacaagatatgatacgcagtgctggtacaattcagagcatatgttctttcctcttttgctttgtttcagacagcaacttttagcagccacgcctctcacaagttgataaaaaaaaattttgtgttgctctcctactactcatcactttctagtgcgatatagtagtagtagtagtaggagtatatgctagcactatccacagtttagcacacagattggtggcacctgggcagatataaaagcttcacatttttatcaatgtctgcgcagttatattgtcgatttgattccttttatagatattactaggtcaagtcgtgtcgcaattactccccccctttcctccattgtagggtgttcctgtttagtccgcgggggatgctgctccctgagcctgtgtgtgtctcttgatttttctttctttgctcgcacgcagcagcagcagcatgttttgtgtcggtgttaaggaccctacataaataattcatcaaatcaagtatcttttgtctgttttggggaatctcctttgcggttcgtccaaaatttcgcgaaagaaacaattctatatgactgcttttgtcgagaggttagacatcactgctagagtagtagagcaatgctacacgtactaaatttttcttactaaacttttactaataatcatctcaaccgtttgatttaagtagatggaatttacaaaaaatctagatgcactcgtagcatgacacatcagtgttagtaagaatttagtaagaaaaagttagtacgtatagccttttccagagtagtactagcagagtgttagattgtatcacctgctcgggcagttgtaaatggtatggtgagttggtgactggtttttacagttttttcatacttgggtttatggaggatttactgttatggagacctgtaaaaaatgttacatatattgctactttctcatttggatttcggatggctgttttttttatcattatgttggtaaagttttgcatcaagaatctgaaccagcttagcaatactaatagtagatggttcagtaagctgcttccatgtccttgcaactttgcaatgctggcaattttttttcgaaaaagagcaaggctggcaatatgtgtatagcttttgtttggcaatatatgttgcgattctagcgctggtggtatctcaagatcagaaaaagtgagcatcaatgtcttactttattggttttctgactgattacattagtaaacgctgtgtccaaaagtatagagtaatactagttatattttcttaattgtcagtgactcagtatccgcagatggtttgtaaagtgcccttatttttcatggtcgttagctttagtaggtatatagtgtactcttgatggtttatcctttttttcgcccctttctcctttgtcatctgggcgcacgttgctatgcagtttcatgagatggttagtgcgtatccaactattctgtcggcaataaagttccacaatcttcaaaattttctaccattctgatgccaactcctactgcactatcagctcttggtttccagctcagttagtggttagaaattgcagcctacatgaaaaagtaactaatagattttctcgtctccaccttttatagttattttcagtgactcttcttccaaaccaagtggtaaagccaggattctaccattacaatgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcaggtactgtacccattgcttgaaatttgtcaccttattatgcttatcgtttactagtttatgataataaatgttcatagtctggcttcttctgtggctttcatacagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccggtaaggatctctgatttattattatttatgctcaagtaatgctgctttttctgaatgttcaacaaactttgatgatcaggttcaacatgagttattgtttttatacactgttttgcatgctattaacttgttgcttctttgtgtattttttactctgcatcccagaatattagaataaccttctgttttgtgtttacagtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtatattctctcttttctatctattctgaatcttaaattgggttaaatctccattatctagaagtatagcaatgaaaattctacaaaaagcaaggggcagccatccctagttctgcttctggtgctgttatctggtcagacatgataccaacgggatttatcagaaataactagataagtttgtgtgacatattatttcaaaaaagaaatatttattagtcttgccccttcaatcttaaacctctttttgacaagagctccttattatgtaaaaaacataaaaatcacacggttgcaatgtatacatcaatattattaagcacatgggggagtaattattgtgaatgtttgtggtccactccttgaactctttgctgcttactcattatgattatcttaaaactctatcagatgatatatgtttctttttgtgtatgccaaccatgccagactcaccaataacatttttattctttaatatctctggctaacatacaattattttgttttaggacacagaaagtttacttattacggtgatgtttaagaaacactaaaatctttttcttttttctattttgttctaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttgatacaaagaaacatattagatgctagcagctgcatgtaattacctttttacctcttgtttttcagctttactgtagtctccaccggaagaaaacgtttggtttagtctcttgaataatctattttcttatcctgtttgtgactatctagaatctatgtaaatggcccagctctagtgaaagttatggtgatgttatcaggaaagaaaatgtggtgtttcccgtaacaagtgctgcaaagtattattcagactc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062995.1 RefSeq:Os05g0586200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0586200&diff=169578 2014-05-18T19:22:06Z

<p>Shixing: Undo revision 169573 by Shixing (talk)</p> <hr /> <div>''OsJAR1'',one kind of Jasmonoyl-isoleucine (JA-Ile) synthase,belongs to class I GH3 progein family in rice,and it is required for the response to wounding.<br /> ==Annotated Information==<br /> ===Function===<br /> [[File:Expression.figure1.png|thumb|right|'''Figure1:'''''Gene expression and hormone content in wild type and OsJAR1 TOS17 insertion lines after simulated herbivory (WOS)(frome reference&lt;ref name=ref1/&gt;).'']]<br /> [[File:Expression.figure2.png|thumb|right|'''Figure2:'''''The expression levels of OsJAR1 and OsJAR2 mRNA and the accumulation of JA and JA-amino acid conjugates in response to wounding(from reference&lt;ref name=ref2/&gt;).'']]<br /> ''OsJAR1'' contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine(JA-Ile) involved in defense responses in rice by the use of an osjar1 Tos17 mutant.&lt;ref name=ref4/&gt;It is involved in phytochrome and jasmonate signalling. In far-red and blue lights, osjar1 coleoptiles were longer if compared with the wild type (WT), indicating that ''OsJAR1'' participates in the suppression of coleoptile elongation in these light conditions, while the mutant did not show a clear phenotype in red light.''OsJar1'' modulates light and JA signalling in the photomorphogenesis of rice.&lt;ref name=ref3/&gt; Light can induce jasmonate-isoleucine conjugation via OsJAR1-dependent pathways in rice. ''OsJAR1'' is the most important JA-Ile conjugating enzyme in the wounding response during the vegetative stage.&lt;ref name=ref5/&gt;Plants produce jasmonic acid (JA) and its amino acid conjugate, jasmonoyl‐L‐isoleucine (JA‐Ile) as major defense signals in response to wounding and herbivory. OsJAR1‐deficient plants have strongly reduced JA‐Ile levels after WOS&lt;ref name=ref1/&gt;.<br /> <br /> ===Expression===<br /> <br /> (1)''OsJar1'' transcripts were up-regulated transiently in response to treatment with exogenous methyl-jasmonic acid (MeJA)&lt;ref name=ref3/&gt;.The expression levels of ''OsJAR1'' and ''OsJAR2'' were induced in response to wounding with the concomitant accumulation of JA-Ile.&lt;ref name=ref2/&gt;<br /> <br /> (2)Real-time PCR was conducted according to the QuantiTect SYBR Green PCR handbook (Qiagen). PCR was perfomed in ABI PRISM 7000 cycler (PE Applied Biosystems, Warrington, UK) using QuantiTect SYBR green-mastermix with HotStar Taq DNA polymerase following the recommended conditions [15 min 95 °C, 40× (15 s, 94 °C; 30 s, 60 °C; 30 s, 72 °C)]. JAR1F2 and JAR1R2 were used as primers to amplify OsJar1 cDNA, Rubg237F and Rubg304R for ubiquitin, which was used as the endogenous control gene.<br /> <br /> For RT-PCR, first-strand cDNA synthesis kit for RT-PCR (Roche, Mannheim, Germany) was performed according to the manufacturer's description. A 1 µL aliquot of a 1:10 dilution of the RT product was used as a template in the subsequent PCR reaction. For amplification of OsJAR1 cDNA, the primers JAR1F1 and JAR1R2 were used, for ubiquitin Rubg237F and Rubg 304R. The PCR conditions were 1 min 94 °C, 30× (94 °C, 1 min; 60 °C, 1 min; 72 °C, 2 min), 72 °C, 7 min.&lt;ref name=ref3/&gt;<br /> <br /> Primers used in real-time PCR and RT-PCR:<br /> <br /> JAR1F1: TAAGGCATTACGCAGGGAAC<br /> <br /> JAR1F2: AGTGCTGAGCATCAACATCG<br /> <br /> JAR1R1: CCAGATAACAGCACCAGAAGC<br /> <br /> JAR1R2: CACCACCAAGGCTTAGGAAA<br /> <br /> Rubg237F: GAGCCTCTGTTCGTCAAGTA<br /> <br /> Rubg304R: ACTCGATGGTCCATTAAACC&lt;ref name=ref3/&gt;<br /> <br /> <br /> ===Evolution===<br /> ''OsJar1'' belongs to the ''GH3'' family in rice. Another member of this family in Arabidopsis, ''DWARF IN LIGHT 2'' (DFL2), showed 47% identities. ''Dfl2'' was shown to be a light-dependent gene, but its gene product did not show enzymatic activity on plant hormones, presumably (Takase et al. 2003).'' OsJAR1'' contained three domains described to be important for the enzymatic function of ''GH3'' proteins (Chang, Xiang &amp; Dunaway-Mariano 1997; Staswick et al. 2002; Terol et al. 2006).&lt;ref name=ref3/&gt;<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> *Biotechnology Research Center, The University of Tokyo, Tokyo 113-8657, Japan.<br /> *Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan<br /> *National Agricultural Research Center of Hokkaido region, National Agriculture and Food Research Organization, Sapporo 062-8555, Japan <br /> *Botanical Institute, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany<br /> *Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena,Germany<br /> *Department of Plant Physiology, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan<br /> *RIKEN Plant Science Center, Tsurumi,Yokohama, Kanagawa 230-0045, Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> * &lt;ref name=&quot;ref1&quot;&gt;<br /> Kaori Fukumoto, Kabir Md Alamgir, Yuko Yamashita, Ivan Galis, et al. (2013) Response of rice to insect elicitors and the role of OsJAR1 in wound and herbivory-induced JA-Ile accumulation[J]. Integr Plant Biol 55: 775–784.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref2&quot;&gt;<br /> Shinji Wakutaa, Erika Suzukia, Wataru Saburia,*, Hideyuki Matsuuraa, Kensuke Nabetaa, Ryozo Imaib and Hirokazu Matsuia (2011) OsJAR1 and OsJAR2 are jasmonyl-L-isoleucine synthases involved in wound- and pathogen-induced jasmonic acid signaling[J].Biochemical and Biophysical Research Communications,409(4): 634-639.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref3&quot;&gt;<br /> MAREN RIEMANN, MICHAEL RIEMANN and MAKOTO TAKANO. (2008) Rice JASMONATE RESISTANT 1 is involved in phytochrome and jasmonate signalling. Plant, Cell &amp; Environment 31(6):783–792.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref4&quot;&gt;<br /> Shimizu T, Miyamoto K, Miyamoto K, Minami E, Nishizawa Y, Iino M, Nojiri H, Yamane H, Okada K. (2013) OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice[J].Bioscience, Biotechnology, and Biochemistry 77(7):1556-1564.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref5&quot;&gt;<br /> KATHARINA SVYATYNA, YUSUKE JIKUMARU, RITA BRENDEL, MICHAEL REICHELT, AXEL MITHÖFER, MAKOTO TAKANO, YUJI KAMIYA, PETER NICK &amp; MICHAEL RIEMANN. (2013) Light induces jasmonate-isoleucine conjugation via OsJAR1-dependent and -independent pathways in rice.Plant, Cell &amp; Environment 37(4):827-839.<br /> &lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0586200|<br /> Description = GH3 auxin-responsive promoter family protein|<br /> Version = NM_001062995.1 GI:115465720 GeneID:4339756|<br /> Length = 5516 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0586200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:29281780..29287295|<br /> CDS = 29284407..29284687,29284793..29284894,29285094..29285911,29286499..29287043|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccgtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MTICSCEETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNF GLGGRTDAESYKSCIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKP KFIPFNDELLETTLQIYRTSYAFRNREYPIGQGKALQFVYGSKQVITKGGILATTATT NLYRRQRYKEGMKDIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAH SLVHAFQTFEEVWEDLCTDIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIG LSNWYGVIPALWPNAKYVYGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNI DPTVPPEQVTYAVLPQVGYFEFIPLEKPIGEETENSASIHYIESDPVGLTEVEVGKIY EVVITNFAGLYRYRLGDVVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVE EASKFLEGEKLEVMDFTSFVERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDA GYTGSRKIKTIGPLELRILRKGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQIL SRNVTQSYFSTAYGF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2628..2908#3014..3115#3315..4132#4720..5264#acccgctctctcctctcatctcctcctcctgtgttcgtgttcttcttcttcttgtagcagcgcttaaccaacccatcattagcgaccaaacctctctccctctccccctctgactaatcaaaattaaacaatttcgatctccaccttcctcttcttgattccagttccaccagagagagacagggtcttctctaatccatgtggtgcttgttggtctcgcttgcttcgtcttcgtcttcgtcttcctcttctcgcacctagtcccgcctcgtcgcctcctccggagaccggagccggacgggacggttgccggcgagcggcggtcgctttcttctctcggccctactcacggtcagtgattggctcccttccttcctggtgttctattgatcctttgtgggggaaaaaaggcttcgatttggggggcatgttcttgcctttttcctgttttgggggagacgagacggatttcaatatatttttttaatctggggggattatgtgtggaaaagattgttcctcgtttatctgggtagtatttggagggtcctatggggatattctttgtaaagctgggatttttaggtggtaaaataatgttgatatactttcagaaagaatatactgcagaaagtttttcagagataacagataagattaggccgtccttaatctcctttctttgcgtctgttccctccaactaggatggaaaagaatggcggattgattagtcagaaactggctgctgaatactctgagatttcatttcatttcagcctctgttgattcctcatggctgtctctaacttctttgcttgtgcttccgatttctttggcggtaaatagcctccacgaatctctggtcaacgaggtattaaaacttaaaagggcttttgtctcaggttgacccgagccatgattcgcccccctcccatgctttgcaaacaagatatgatacgcagtgctggtacaattcagagcatatgttctttcctcttttgctttgtttcagacagcaacttttagcagccacgcctctcacaagttgataaaaaaaaattttgtgttgctctcctactactcatcactttctagtgcgatatagtagtagtagtagtaggagtatatgctagcactatccacagtttagcacacagattggtggcacctgggcagatataaaagcttcacatttttatcaatgtctgcgcagttatattgtcgatttgattccttttatagatattactaggtcaagtcgtgtcgcaattactccccccctttcctccattgtagggtgttcctgtttagtccgcgggggatgctgctccctgagcctgtgtgtgtctcttgatttttctttctttgctcgcacgcagcagcagcagcatgttttgtgtcggtgttaaggaccctacataaataattcatcaaatcaagtatcttttgtctgttttggggaatctcctttgcggttcgtccaaaatttcgcgaaagaaacaattctatatgactgcttttgtcgagaggttagacatcactgctagagtagtagagcaatgctacacgtactaaatttttcttactaaacttttactaataatcatctcaaccgtttgatttaagtagatggaatttacaaaaaatctagatgcactcgtagcatgacacatcagtgttagtaagaatttagtaagaaaaagttagtacgtatagccttttccagagtagtactagcagagtgttagattgtatcacctgctcgggcagttgtaaatggtatggtgagttggtgactggtttttacagttttttcatacttgggtttatggaggatttactgttatggagacctgtaaaaaatgttacatatattgctactttctcatttggatttcggatggctgttttttttatcattatgttggtaaagttttgcatcaagaatctgaaccagcttagcaatactaatagtagatggttcagtaagctgcttccatgtccttgcaactttgcaatgctggcaattttttttcgaaaaagagcaaggctggcaatatgtgtatagcttttgtttggcaatatatgttgcgattctagcgctggtggtatctcaagatcagaaaaagtgagcatcaatgtcttactttattggttttctgactgattacattagtaaacgctgtgtccaaaagtatagagtaatactagttatattttcttaattgtcagtgactcagtatccgcagatggtttgtaaagtgcccttatttttcatggtcgttagctttagtaggtatatagtgtactcttgatggtttatcctttttttcgcccctttctcctttgtcatctgggcgcacgttgctatgcagtttcatgagatggttagtgcgtatccaactattctgtcggcaataaagttccacaatcttcaaaattttctaccattctgatgccaactcctactgcactatcagctcttggtttccagctcagttagtggttagaaattgcagcctacatgaaaaagtaactaatagattttctcgtctccaccttttatagttattttcagtgactcttcttccaaaccaagtggtaaagccaggattctaccattacaatgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcaggtactgtacccattgcttgaaatttgtcaccttattatgcttatcgtttactagtttatgataataaatgttcatagtctggcttcttctgtggctttcatacagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccggtaaggatctctgatttattattatttatgctcaagtaatgctgctttttctgaatgttcaacaaactttgatgatcaggttcaacatgagttattgtttttatacactgttttgcatgctattaacttgttgcttctttgtgtattttttactctgcatcccagaatattagaataaccttctgttttgtgtttacagtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtatattctctcttttctatctattctgaatcttaaattgggttaaatctccattatctagaagtatagcaatgaaaattctacaaaaagcaaggggcagccatccctagttctgcttctggtgctgttatctggtcagacatgataccaacgggatttatcagaaataactagataagtttgtgtgacatattatttcaaaaaagaaatatttattagtcttgccccttcaatcttaaacctctttttgacaagagctccttattatgtaaaaaacataaaaatcacacggttgcaatgtatacatcaatattattaagcacatgggggagtaattattgtgaatgtttgtggtccactccttgaactctttgctgcttactcattatgattatcttaaaactctatcagatgatatatgtttctttttgtgtatgccaaccatgccagactcaccaataacatttttattctttaatatctctggctaacatacaattattttgttttaggacacagaaagtttacttattacggtgatgtttaagaaacactaaaatctttttcttttttctattttgttctaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttgatacaaagaaacatattagatgctagcagctgcatgtaattacctttttacctcttgtttttcagctttactgtagtctccaccggaagaaaacgtttggtttagtctcttgaataatctattttcttatcctgtttgtgactatctagaatctatgtaaatggcccagctctagtgaaagttatggtgatgttatcaggaaagaaaatgtggtgtttcccgtaacaagtgctgcaaagtattattcagactc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062995.1 RefSeq:Os05g0586200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169577 2014-05-18T19:18:48Z

<p>Shixing: /* Annotated Information */</p> <hr /> <div>''OsbHLH148'' is a basic helix-loop-helix protein, and it interacts with OsJAZ proteins in a jasmonate signalling pathway leading to drought tolerance in rice.<br /> ==Annotated Information==<br /> ===Function===<br /> ''OsbHLH148'' interacts with ''OsJAZ'' proteins in a jasmonate signalling pathway leading to drought tolerance in rice. The over-expressed ''OsbHLH148'' can cause the up-regulation of some genes involved in stress responses and the jasmonate signalling pathway such as OsDREB and OsJAZ genes. ''OsbHLH14''8 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signalling module in the upstream signalling pathway to improve drought tolerance in rice&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> <br /> As figure1 shows, ''OsbHLH148'' transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding&lt;ref name=&quot;ref1&quot; /&gt;。[[File:Expression.gif|thumb|right|''Figure1: OsDREB148 was rapidly induced by 15 min of exposure to MeJA and was gradually responsive to drought conditions（from reference &lt;ref name=&quot;ref1&quot; /&gt;）.'']].<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> *Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> *Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> *GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> *School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Ju-Seok Seo1, Joungsu Joo2, Min-Jeong Kim1, Yang Do Choi1 et al.(2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.The Plant Journal<br /> Volume 65, Issue 6:907–921.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169576 2014-05-18T19:17:42Z

<p>Shixing: </p> <hr /> <div>''OsbHLH148'' is a basic helix-loop-helix protein, and it interacts with OsJAZ proteins in a jasmonate signalling pathway leading to drought tolerance in rice.<br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 interacts with OsJAZ proteins in a jasmonate signalling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signalling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signalling module in the upstream signalling pathway to improve drought tolerance in rice&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> <br /> As figure1 shows, OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding&lt;ref name=&quot;ref1&quot; /&gt;。[[File:Expression.gif|thumb|right|''Figure1: OsDREB148 was rapidly induced by 15 min of exposure to MeJA and was gradually responsive to drought conditions（from reference &lt;ref name=&quot;ref1&quot; /&gt;）.'']].<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> *Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> *Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> *GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> *School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Ju-Seok Seo1, Joungsu Joo2, Min-Jeong Kim1, Yang Do Choi1 et al.(2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.The Plant Journal<br /> Volume 65, Issue 6:907–921.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169575 2014-05-18T19:16:20Z

<p>Shixing: Undo revision 169574 by Shixing (talk)</p> <hr /> <div>''''OsbHLH148'''' is a basic helix-loop-helix protein, and it interacts with OsJAZ proteins in a jasmonate signalling pathway leading to drought tolerance in rice.<br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 interacts with OsJAZ proteins in a jasmonate signalling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signalling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signalling module in the upstream signalling pathway to improve drought tolerance in rice&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> <br /> As figure1 shows, OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding&lt;ref name=&quot;ref1&quot; /&gt;。[[File:Expression.gif|thumb|right|''Figure1: OsDREB148 was rapidly induced by 15 min of exposure to MeJA and was gradually responsive to drought conditions（from reference &lt;ref name=&quot;ref1&quot; /&gt;）.'']].<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> *Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> *Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> *GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> *School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Ju-Seok Seo1, Joungsu Joo2, Min-Jeong Kim1, Yang Do Choi1 et al.(2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.The Plant Journal<br /> Volume 65, Issue 6:907–921.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169574 2014-05-18T19:08:17Z

<p>Shixing: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> good<br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 is a basic helix-loop-helix protein, which interacts with OsJAZ proteins in a jasmonate signalling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signalling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signalling module in the upstream signalling pathway to improve drought tolerance in rice&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> <br /> As figure1 shows, OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding&lt;ref name=&quot;ref1&quot; /&gt;。[[File:Expression.gif|thumb|right|''Figure1: OsDREB148 was rapidly induced by 15 min of exposure to MeJA and was gradually responsive to drought conditions（from reference &lt;ref name=&quot;ref1&quot; /&gt;）.'']].<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> *Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> *Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> *GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> *School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Ju-Seok Seo1, Joungsu Joo2, Min-Jeong Kim1, Yang Do Choi1 et al.(2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.The Plant Journal<br /> Volume 65, Issue 6:907–921.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0586200&diff=169573 2014-05-18T19:06:37Z

<p>Shixing: </p> <hr /> <div>''''OsJAR1'''',one kind of JA-Ile synthase,belongs to class I GH3 progein family in rice,and it is required for the response to wounding.<br /> ==Annotated Information==<br /> ===Function===<br /> [[File:Expression.figure1.png|thumb|right|'''Figure1:'''''Gene expression and hormone content in wild type and OsJAR1 TOS17 insertion lines after simulated herbivory (WOS)(frome reference&lt;ref name=ref1/&gt;).'']]<br /> [[File:Expression.figure2.png|thumb|right|'''Figure2:'''''The expression levels of OsJAR1 and OsJAR2 mRNA and the accumulation of JA and JA-amino acid conjugates in response to wounding(from reference&lt;ref name=ref2/&gt;).'']]<br /> OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine(JA-Ile) involved in defense responses in rice by the use of an osjar1 Tos17 mutant.&lt;ref name=ref4/&gt;It is involved in phytochrome and jasmonate signalling. In far-red and blue lights, osjar1 coleoptiles were longer if compared with the wild type (WT), indicating that OsJAR1 participates in the suppression of coleoptile elongation in these light conditions, while the mutant did not show a clear phenotype in red light.OsJar1 modulates light and JA signalling in the photomorphogenesis of rice.&lt;ref name=ref3/&gt; Light can induce jasmonate-isoleucine conjugation via OsJAR1-dependent pathways in rice. OsJAR1 is the most important JA-Ile conjugating enzyme in the wounding response during the vegetative stage.&lt;ref name=ref5/&gt;Plants produce jasmonic acid (JA) and its amino acid conjugate, jasmonoyl‐L‐isoleucine (JA‐Ile) as major defense signals in response to wounding and herbivory. OsJAR1‐deficient plants have strongly reduced JA‐Ile levels after WOS&lt;ref name=ref1/&gt;.<br /> <br /> ===Expression===<br /> <br /> (1)OsJar1 transcripts were up-regulated transiently in response to treatment with exogenous methyl-jasmonic acid (MeJA)&lt;ref name=ref3/&gt;.The expression levels of OsJAR1 and OsJAR2 were induced in response to wounding with the concomitant accumulation of JA-Ile.&lt;ref name=ref2/&gt;<br /> <br /> (2)Real-time PCR was conducted according to the QuantiTect SYBR Green PCR handbook (Qiagen). PCR was perfomed in ABI PRISM 7000 cycler (PE Applied Biosystems, Warrington, UK) using QuantiTect SYBR green-mastermix with HotStar Taq DNA polymerase following the recommended conditions [15 min 95 °C, 40× (15 s, 94 °C; 30 s, 60 °C; 30 s, 72 °C)]. JAR1F2 and JAR1R2 were used as primers to amplify OsJar1 cDNA, Rubg237F and Rubg304R for ubiquitin, which was used as the endogenous control gene.<br /> <br /> For RT-PCR, first-strand cDNA synthesis kit for RT-PCR (Roche, Mannheim, Germany) was performed according to the manufacturer's description. A 1 µL aliquot of a 1:10 dilution of the RT product was used as a template in the subsequent PCR reaction. For amplification of OsJAR1 cDNA, the primers JAR1F1 and JAR1R2 were used, for ubiquitin Rubg237F and Rubg 304R. The PCR conditions were 1 min 94 °C, 30× (94 °C, 1 min; 60 °C, 1 min; 72 °C, 2 min), 72 °C, 7 min.&lt;ref name=ref3/&gt;<br /> <br /> Primers used in real-time PCR and RT-PCR:<br /> <br /> JAR1F1: TAAGGCATTACGCAGGGAAC<br /> <br /> JAR1F2: AGTGCTGAGCATCAACATCG<br /> <br /> JAR1R1: CCAGATAACAGCACCAGAAGC<br /> <br /> JAR1R2: CACCACCAAGGCTTAGGAAA<br /> <br /> Rubg237F: GAGCCTCTGTTCGTCAAGTA<br /> <br /> Rubg304R: ACTCGATGGTCCATTAAACC&lt;ref name=ref3/&gt;<br /> <br /> <br /> ===Evolution===<br /> OsJar1 belongs to the GH3 family in rice. Another member of this family in Arabidopsis, DWARF IN LIGHT 2 (DFL2), showed 47% identities. Dfl2 was shown to be a light-dependent gene, but its gene product did not show enzymatic activity on plant hormones, presumably (Takase et al. 2003). OsJAR1 contained three domains described to be important for the enzymatic function of GH3 proteins (Chang, Xiang &amp; Dunaway-Mariano 1997; Staswick et al. 2002; Terol et al. 2006).&lt;ref name=ref3/&gt;<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> *Biotechnology Research Center, The University of Tokyo, Tokyo 113-8657, Japan.<br /> *Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan<br /> *National Agricultural Research Center of Hokkaido region, National Agriculture and Food Research Organization, Sapporo 062-8555, Japan <br /> *Botanical Institute, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany<br /> *Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena,Germany<br /> *Department of Plant Physiology, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan<br /> *RIKEN Plant Science Center, Tsurumi,Yokohama, Kanagawa 230-0045, Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> * &lt;ref name=&quot;ref1&quot;&gt;<br /> Kaori Fukumoto, Kabir Md Alamgir, Yuko Yamashita, Ivan Galis, et al. (2013) Response of rice to insect elicitors and the role of OsJAR1 in wound and herbivory-induced JA-Ile accumulation[J]. Integr Plant Biol 55: 775–784.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref2&quot;&gt;<br /> Shinji Wakutaa, Erika Suzukia, Wataru Saburia,*, Hideyuki Matsuuraa, Kensuke Nabetaa, Ryozo Imaib and Hirokazu Matsuia (2011) OsJAR1 and OsJAR2 are jasmonyl-L-isoleucine synthases involved in wound- and pathogen-induced jasmonic acid signaling[J].Biochemical and Biophysical Research Communications,409(4): 634-639.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref3&quot;&gt;<br /> MAREN RIEMANN, MICHAEL RIEMANN and MAKOTO TAKANO. (2008) Rice JASMONATE RESISTANT 1 is involved in phytochrome and jasmonate signalling. Plant, Cell &amp; Environment 31(6):783–792.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref4&quot;&gt;<br /> Shimizu T, Miyamoto K, Miyamoto K, Minami E, Nishizawa Y, Iino M, Nojiri H, Yamane H, Okada K. (2013) OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice[J].Bioscience, Biotechnology, and Biochemistry 77(7):1556-1564.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref5&quot;&gt;<br /> KATHARINA SVYATYNA, YUSUKE JIKUMARU, RITA BRENDEL, MICHAEL REICHELT, AXEL MITHÖFER, MAKOTO TAKANO, YUJI KAMIYA, PETER NICK &amp; MICHAEL RIEMANN. (2013) Light induces jasmonate-isoleucine conjugation via OsJAR1-dependent and -independent pathways in rice.Plant, Cell &amp; Environment 37(4):827-839.<br /> &lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0586200|<br /> Description = GH3 auxin-responsive promoter family protein|<br /> Version = NM_001062995.1 GI:115465720 GeneID:4339756|<br /> Length = 5516 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0586200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:29281780..29287295|<br /> CDS = 29284407..29284687,29284793..29284894,29285094..29285911,29286499..29287043|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccgtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MTICSCEETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNF GLGGRTDAESYKSCIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKP KFIPFNDELLETTLQIYRTSYAFRNREYPIGQGKALQFVYGSKQVITKGGILATTATT NLYRRQRYKEGMKDIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAH SLVHAFQTFEEVWEDLCTDIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIG LSNWYGVIPALWPNAKYVYGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNI DPTVPPEQVTYAVLPQVGYFEFIPLEKPIGEETENSASIHYIESDPVGLTEVEVGKIY EVVITNFAGLYRYRLGDVVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVE EASKFLEGEKLEVMDFTSFVERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDA GYTGSRKIKTIGPLELRILRKGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQIL SRNVTQSYFSTAYGF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2628..2908#3014..3115#3315..4132#4720..5264#acccgctctctcctctcatctcctcctcctgtgttcgtgttcttcttcttcttgtagcagcgcttaaccaacccatcattagcgaccaaacctctctccctctccccctctgactaatcaaaattaaacaatttcgatctccaccttcctcttcttgattccagttccaccagagagagacagggtcttctctaatccatgtggtgcttgttggtctcgcttgcttcgtcttcgtcttcgtcttcctcttctcgcacctagtcccgcctcgtcgcctcctccggagaccggagccggacgggacggttgccggcgagcggcggtcgctttcttctctcggccctactcacggtcagtgattggctcccttccttcctggtgttctattgatcctttgtgggggaaaaaaggcttcgatttggggggcatgttcttgcctttttcctgttttgggggagacgagacggatttcaatatatttttttaatctggggggattatgtgtggaaaagattgttcctcgtttatctgggtagtatttggagggtcctatggggatattctttgtaaagctgggatttttaggtggtaaaataatgttgatatactttcagaaagaatatactgcagaaagtttttcagagataacagataagattaggccgtccttaatctcctttctttgcgtctgttccctccaactaggatggaaaagaatggcggattgattagtcagaaactggctgctgaatactctgagatttcatttcatttcagcctctgttgattcctcatggctgtctctaacttctttgcttgtgcttccgatttctttggcggtaaatagcctccacgaatctctggtcaacgaggtattaaaacttaaaagggcttttgtctcaggttgacccgagccatgattcgcccccctcccatgctttgcaaacaagatatgatacgcagtgctggtacaattcagagcatatgttctttcctcttttgctttgtttcagacagcaacttttagcagccacgcctctcacaagttgataaaaaaaaattttgtgttgctctcctactactcatcactttctagtgcgatatagtagtagtagtagtaggagtatatgctagcactatccacagtttagcacacagattggtggcacctgggcagatataaaagcttcacatttttatcaatgtctgcgcagttatattgtcgatttgattccttttatagatattactaggtcaagtcgtgtcgcaattactccccccctttcctccattgtagggtgttcctgtttagtccgcgggggatgctgctccctgagcctgtgtgtgtctcttgatttttctttctttgctcgcacgcagcagcagcagcatgttttgtgtcggtgttaaggaccctacataaataattcatcaaatcaagtatcttttgtctgttttggggaatctcctttgcggttcgtccaaaatttcgcgaaagaaacaattctatatgactgcttttgtcgagaggttagacatcactgctagagtagtagagcaatgctacacgtactaaatttttcttactaaacttttactaataatcatctcaaccgtttgatttaagtagatggaatttacaaaaaatctagatgcactcgtagcatgacacatcagtgttagtaagaatttagtaagaaaaagttagtacgtatagccttttccagagtagtactagcagagtgttagattgtatcacctgctcgggcagttgtaaatggtatggtgagttggtgactggtttttacagttttttcatacttgggtttatggaggatttactgttatggagacctgtaaaaaatgttacatatattgctactttctcatttggatttcggatggctgttttttttatcattatgttggtaaagttttgcatcaagaatctgaaccagcttagcaatactaatagtagatggttcagtaagctgcttccatgtccttgcaactttgcaatgctggcaattttttttcgaaaaagagcaaggctggcaatatgtgtatagcttttgtttggcaatatatgttgcgattctagcgctggtggtatctcaagatcagaaaaagtgagcatcaatgtcttactttattggttttctgactgattacattagtaaacgctgtgtccaaaagtatagagtaatactagttatattttcttaattgtcagtgactcagtatccgcagatggtttgtaaagtgcccttatttttcatggtcgttagctttagtaggtatatagtgtactcttgatggtttatcctttttttcgcccctttctcctttgtcatctgggcgcacgttgctatgcagtttcatgagatggttagtgcgtatccaactattctgtcggcaataaagttccacaatcttcaaaattttctaccattctgatgccaactcctactgcactatcagctcttggtttccagctcagttagtggttagaaattgcagcctacatgaaaaagtaactaatagattttctcgtctccaccttttatagttattttcagtgactcttcttccaaaccaagtggtaaagccaggattctaccattacaatgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcaggtactgtacccattgcttgaaatttgtcaccttattatgcttatcgtttactagtttatgataataaatgttcatagtctggcttcttctgtggctttcatacagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccggtaaggatctctgatttattattatttatgctcaagtaatgctgctttttctgaatgttcaacaaactttgatgatcaggttcaacatgagttattgtttttatacactgttttgcatgctattaacttgttgcttctttgtgtattttttactctgcatcccagaatattagaataaccttctgttttgtgtttacagtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtatattctctcttttctatctattctgaatcttaaattgggttaaatctccattatctagaagtatagcaatgaaaattctacaaaaagcaaggggcagccatccctagttctgcttctggtgctgttatctggtcagacatgataccaacgggatttatcagaaataactagataagtttgtgtgacatattatttcaaaaaagaaatatttattagtcttgccccttcaatcttaaacctctttttgacaagagctccttattatgtaaaaaacataaaaatcacacggttgcaatgtatacatcaatattattaagcacatgggggagtaattattgtgaatgtttgtggtccactccttgaactctttgctgcttactcattatgattatcttaaaactctatcagatgatatatgtttctttttgtgtatgccaaccatgccagactcaccaataacatttttattctttaatatctctggctaacatacaattattttgttttaggacacagaaagtttacttattacggtgatgtttaagaaacactaaaatctttttcttttttctattttgttctaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttgatacaaagaaacatattagatgctagcagctgcatgtaattacctttttacctcttgtttttcagctttactgtagtctccaccggaagaaaacgtttggtttagtctcttgaataatctattttcttatcctgtttgtgactatctagaatctatgtaaatggcccagctctagtgaaagttatggtgatgttatcaggaaagaaaatgtggtgtttcccgtaacaagtgctgcaaagtattattcagactc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062995.1 RefSeq:Os05g0586200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0586200&diff=169572 2014-05-18T19:05:06Z

<p>Shixing: Undo revision 169571 by Shixing (talk)</p> <hr /> <div>''''OsJAR1'''',one kind of Jasmonoyl-isoleucine (JA-Ile) synthase,belongs to class I GH3 progein family in rice,and it is required for the response to wounding.<br /> ==Annotated Information==<br /> ===Function===<br /> [[File:Expression.figure1.png|thumb|right|'''Figure1:'''''Gene expression and hormone content in wild type and OsJAR1 TOS17 insertion lines after simulated herbivory (WOS)(frome reference&lt;ref name=ref1/&gt;).'']]<br /> [[File:Expression.figure2.png|thumb|right|'''Figure2:'''''The expression levels of OsJAR1 and OsJAR2 mRNA and the accumulation of JA and JA-amino acid conjugates in response to wounding(from reference&lt;ref name=ref2/&gt;).'']]<br /> OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine(JA-Ile) involved in defense responses in rice by the use of an osjar1 Tos17 mutant.&lt;ref name=ref4/&gt;It is involved in phytochrome and jasmonate signalling. In far-red and blue lights, osjar1 coleoptiles were longer if compared with the wild type (WT), indicating that OsJAR1 participates in the suppression of coleoptile elongation in these light conditions, while the mutant did not show a clear phenotype in red light.OsJar1 modulates light and JA signalling in the photomorphogenesis of rice.&lt;ref name=ref3/&gt; Light can induce jasmonate-isoleucine conjugation via OsJAR1-dependent pathways in rice. OsJAR1 is the most important JA-Ile conjugating enzyme in the wounding response during the vegetative stage.&lt;ref name=ref5/&gt;Plants produce jasmonic acid (JA) and its amino acid conjugate, jasmonoyl‐L‐isoleucine (JA‐Ile) as major defense signals in response to wounding and herbivory. OsJAR1‐deficient plants have strongly reduced JA‐Ile levels after WOS&lt;ref name=ref1/&gt;.<br /> <br /> ===Expression===<br /> <br /> (1)OsJar1 transcripts were up-regulated transiently in response to treatment with exogenous methyl-jasmonic acid (MeJA)&lt;ref name=ref3/&gt;.The expression levels of OsJAR1 and OsJAR2 were induced in response to wounding with the concomitant accumulation of JA-Ile.&lt;ref name=ref2/&gt;<br /> <br /> (2)Real-time PCR was conducted according to the QuantiTect SYBR Green PCR handbook (Qiagen). PCR was perfomed in ABI PRISM 7000 cycler (PE Applied Biosystems, Warrington, UK) using QuantiTect SYBR green-mastermix with HotStar Taq DNA polymerase following the recommended conditions [15 min 95 °C, 40× (15 s, 94 °C; 30 s, 60 °C; 30 s, 72 °C)]. JAR1F2 and JAR1R2 were used as primers to amplify OsJar1 cDNA, Rubg237F and Rubg304R for ubiquitin, which was used as the endogenous control gene.<br /> <br /> For RT-PCR, first-strand cDNA synthesis kit for RT-PCR (Roche, Mannheim, Germany) was performed according to the manufacturer's description. A 1 µL aliquot of a 1:10 dilution of the RT product was used as a template in the subsequent PCR reaction. For amplification of OsJAR1 cDNA, the primers JAR1F1 and JAR1R2 were used, for ubiquitin Rubg237F and Rubg 304R. The PCR conditions were 1 min 94 °C, 30× (94 °C, 1 min; 60 °C, 1 min; 72 °C, 2 min), 72 °C, 7 min.&lt;ref name=ref3/&gt;<br /> <br /> Primers used in real-time PCR and RT-PCR:<br /> <br /> JAR1F1: TAAGGCATTACGCAGGGAAC<br /> <br /> JAR1F2: AGTGCTGAGCATCAACATCG<br /> <br /> JAR1R1: CCAGATAACAGCACCAGAAGC<br /> <br /> JAR1R2: CACCACCAAGGCTTAGGAAA<br /> <br /> Rubg237F: GAGCCTCTGTTCGTCAAGTA<br /> <br /> Rubg304R: ACTCGATGGTCCATTAAACC&lt;ref name=ref3/&gt;<br /> <br /> <br /> ===Evolution===<br /> OsJar1 belongs to the GH3 family in rice. Another member of this family in Arabidopsis, DWARF IN LIGHT 2 (DFL2), showed 47% identities. Dfl2 was shown to be a light-dependent gene, but its gene product did not show enzymatic activity on plant hormones, presumably (Takase et al. 2003). OsJAR1 contained three domains described to be important for the enzymatic function of GH3 proteins (Chang, Xiang &amp; Dunaway-Mariano 1997; Staswick et al. 2002; Terol et al. 2006).&lt;ref name=ref3/&gt;<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> *Biotechnology Research Center, The University of Tokyo, Tokyo 113-8657, Japan.<br /> *Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan<br /> *National Agricultural Research Center of Hokkaido region, National Agriculture and Food Research Organization, Sapporo 062-8555, Japan <br /> *Botanical Institute, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany<br /> *Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena,Germany<br /> *Department of Plant Physiology, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan<br /> *RIKEN Plant Science Center, Tsurumi,Yokohama, Kanagawa 230-0045, Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> * &lt;ref name=&quot;ref1&quot;&gt;<br /> Kaori Fukumoto, Kabir Md Alamgir, Yuko Yamashita, Ivan Galis, et al. (2013) Response of rice to insect elicitors and the role of OsJAR1 in wound and herbivory-induced JA-Ile accumulation[J]. Integr Plant Biol 55: 775–784.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref2&quot;&gt;<br /> Shinji Wakutaa, Erika Suzukia, Wataru Saburia,*, Hideyuki Matsuuraa, Kensuke Nabetaa, Ryozo Imaib and Hirokazu Matsuia (2011) OsJAR1 and OsJAR2 are jasmonyl-L-isoleucine synthases involved in wound- and pathogen-induced jasmonic acid signaling[J].Biochemical and Biophysical Research Communications,409(4): 634-639.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref3&quot;&gt;<br /> MAREN RIEMANN, MICHAEL RIEMANN and MAKOTO TAKANO. (2008) Rice JASMONATE RESISTANT 1 is involved in phytochrome and jasmonate signalling. Plant, Cell &amp; Environment 31(6):783–792.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref4&quot;&gt;<br /> Shimizu T, Miyamoto K, Miyamoto K, Minami E, Nishizawa Y, Iino M, Nojiri H, Yamane H, Okada K. (2013) OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice[J].Bioscience, Biotechnology, and Biochemistry 77(7):1556-1564.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref5&quot;&gt;<br /> KATHARINA SVYATYNA, YUSUKE JIKUMARU, RITA BRENDEL, MICHAEL REICHELT, AXEL MITHÖFER, MAKOTO TAKANO, YUJI KAMIYA, PETER NICK &amp; MICHAEL RIEMANN. (2013) Light induces jasmonate-isoleucine conjugation via OsJAR1-dependent and -independent pathways in rice.Plant, Cell &amp; Environment 37(4):827-839.<br /> &lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0586200|<br /> Description = GH3 auxin-responsive promoter family protein|<br /> Version = NM_001062995.1 GI:115465720 GeneID:4339756|<br /> Length = 5516 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0586200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:29281780..29287295|<br /> CDS = 29284407..29284687,29284793..29284894,29285094..29285911,29286499..29287043|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccgtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MTICSCEETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNF GLGGRTDAESYKSCIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKP KFIPFNDELLETTLQIYRTSYAFRNREYPIGQGKALQFVYGSKQVITKGGILATTATT NLYRRQRYKEGMKDIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAH SLVHAFQTFEEVWEDLCTDIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIG LSNWYGVIPALWPNAKYVYGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNI DPTVPPEQVTYAVLPQVGYFEFIPLEKPIGEETENSASIHYIESDPVGLTEVEVGKIY EVVITNFAGLYRYRLGDVVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVE EASKFLEGEKLEVMDFTSFVERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDA GYTGSRKIKTIGPLELRILRKGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQIL SRNVTQSYFSTAYGF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2628..2908#3014..3115#3315..4132#4720..5264#acccgctctctcctctcatctcctcctcctgtgttcgtgttcttcttcttcttgtagcagcgcttaaccaacccatcattagcgaccaaacctctctccctctccccctctgactaatcaaaattaaacaatttcgatctccaccttcctcttcttgattccagttccaccagagagagacagggtcttctctaatccatgtggtgcttgttggtctcgcttgcttcgtcttcgtcttcgtcttcctcttctcgcacctagtcccgcctcgtcgcctcctccggagaccggagccggacgggacggttgccggcgagcggcggtcgctttcttctctcggccctactcacggtcagtgattggctcccttccttcctggtgttctattgatcctttgtgggggaaaaaaggcttcgatttggggggcatgttcttgcctttttcctgttttgggggagacgagacggatttcaatatatttttttaatctggggggattatgtgtggaaaagattgttcctcgtttatctgggtagtatttggagggtcctatggggatattctttgtaaagctgggatttttaggtggtaaaataatgttgatatactttcagaaagaatatactgcagaaagtttttcagagataacagataagattaggccgtccttaatctcctttctttgcgtctgttccctccaactaggatggaaaagaatggcggattgattagtcagaaactggctgctgaatactctgagatttcatttcatttcagcctctgttgattcctcatggctgtctctaacttctttgcttgtgcttccgatttctttggcggtaaatagcctccacgaatctctggtcaacgaggtattaaaacttaaaagggcttttgtctcaggttgacccgagccatgattcgcccccctcccatgctttgcaaacaagatatgatacgcagtgctggtacaattcagagcatatgttctttcctcttttgctttgtttcagacagcaacttttagcagccacgcctctcacaagttgataaaaaaaaattttgtgttgctctcctactactcatcactttctagtgcgatatagtagtagtagtagtaggagtatatgctagcactatccacagtttagcacacagattggtggcacctgggcagatataaaagcttcacatttttatcaatgtctgcgcagttatattgtcgatttgattccttttatagatattactaggtcaagtcgtgtcgcaattactccccccctttcctccattgtagggtgttcctgtttagtccgcgggggatgctgctccctgagcctgtgtgtgtctcttgatttttctttctttgctcgcacgcagcagcagcagcatgttttgtgtcggtgttaaggaccctacataaataattcatcaaatcaagtatcttttgtctgttttggggaatctcctttgcggttcgtccaaaatttcgcgaaagaaacaattctatatgactgcttttgtcgagaggttagacatcactgctagagtagtagagcaatgctacacgtactaaatttttcttactaaacttttactaataatcatctcaaccgtttgatttaagtagatggaatttacaaaaaatctagatgcactcgtagcatgacacatcagtgttagtaagaatttagtaagaaaaagttagtacgtatagccttttccagagtagtactagcagagtgttagattgtatcacctgctcgggcagttgtaaatggtatggtgagttggtgactggtttttacagttttttcatacttgggtttatggaggatttactgttatggagacctgtaaaaaatgttacatatattgctactttctcatttggatttcggatggctgttttttttatcattatgttggtaaagttttgcatcaagaatctgaaccagcttagcaatactaatagtagatggttcagtaagctgcttccatgtccttgcaactttgcaatgctggcaattttttttcgaaaaagagcaaggctggcaatatgtgtatagcttttgtttggcaatatatgttgcgattctagcgctggtggtatctcaagatcagaaaaagtgagcatcaatgtcttactttattggttttctgactgattacattagtaaacgctgtgtccaaaagtatagagtaatactagttatattttcttaattgtcagtgactcagtatccgcagatggtttgtaaagtgcccttatttttcatggtcgttagctttagtaggtatatagtgtactcttgatggtttatcctttttttcgcccctttctcctttgtcatctgggcgcacgttgctatgcagtttcatgagatggttagtgcgtatccaactattctgtcggcaataaagttccacaatcttcaaaattttctaccattctgatgccaactcctactgcactatcagctcttggtttccagctcagttagtggttagaaattgcagcctacatgaaaaagtaactaatagattttctcgtctccaccttttatagttattttcagtgactcttcttccaaaccaagtggtaaagccaggattctaccattacaatgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcaggtactgtacccattgcttgaaatttgtcaccttattatgcttatcgtttactagtttatgataataaatgttcatagtctggcttcttctgtggctttcatacagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccggtaaggatctctgatttattattatttatgctcaagtaatgctgctttttctgaatgttcaacaaactttgatgatcaggttcaacatgagttattgtttttatacactgttttgcatgctattaacttgttgcttctttgtgtattttttactctgcatcccagaatattagaataaccttctgttttgtgtttacagtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtatattctctcttttctatctattctgaatcttaaattgggttaaatctccattatctagaagtatagcaatgaaaattctacaaaaagcaaggggcagccatccctagttctgcttctggtgctgttatctggtcagacatgataccaacgggatttatcagaaataactagataagtttgtgtgacatattatttcaaaaaagaaatatttattagtcttgccccttcaatcttaaacctctttttgacaagagctccttattatgtaaaaaacataaaaatcacacggttgcaatgtatacatcaatattattaagcacatgggggagtaattattgtgaatgtttgtggtccactccttgaactctttgctgcttactcattatgattatcttaaaactctatcagatgatatatgtttctttttgtgtatgccaaccatgccagactcaccaataacatttttattctttaatatctctggctaacatacaattattttgttttaggacacagaaagtttacttattacggtgatgtttaagaaacactaaaatctttttcttttttctattttgttctaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttgatacaaagaaacatattagatgctagcagctgcatgtaattacctttttacctcttgtttttcagctttactgtagtctccaccggaagaaaacgtttggtttagtctcttgaataatctattttcttatcctgtttgtgactatctagaatctatgtaaatggcccagctctagtgaaagttatggtgatgttatcaggaaagaaaatgtggtgtttcccgtaacaagtgctgcaaagtattattcagactc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062995.1 RefSeq:Os05g0586200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0586200&diff=169571 2014-05-18T18:35:37Z

<p>Shixing: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information<br /> haha<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> [[File:Expression.figure1.png|thumb|right|'''Figure1:'''''Gene expression and hormone content in wild type and OsJAR1 TOS17 insertion lines after simulated herbivory (WOS)(frome reference&lt;ref name=ref1/&gt;).'']]<br /> [[File:Expression.figure2.png|thumb|right|'''Figure2:'''''The expression levels of OsJAR1 and OsJAR2 mRNA and the accumulation of JA and JA-amino acid conjugates in response to wounding(from reference&lt;ref name=ref2/&gt;).'']]<br /> OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice by the use of an osjar1 Tos17 mutant.&lt;ref name=ref1/&gt;&lt;ref name=ref4/&gt;It is involved in phytochrome and jasmonate signalling. In far-red and blue lights, osjar1 coleoptiles were longer if compared with the wild type (WT), indicating that OsJar1participates in the suppression of coleoptile elongation in these light conditions, while the mutant did not show a clear phenotype in red light.OsJar1 modulates light and JA signalling in the photomorphogenesis of rice.&lt;ref name=ref3/&gt; Light can induce jasmonate-isoleucine conjugation via OsJAR1-dependent pathways in rice. OsJAR1 is the most important JA-Ile conjugating enzyme in the wounding response during the vegetative stage.&lt;ref name=ref5/&gt;Plants produce jasmonic acid (JA) and its amino acid conjugate, jasmonoyl‐L‐isoleucine (JA‐Ile) as major defense signals in response to wounding and herbivory. OsJAR1‐deficient plants have strongly reduced JA‐Ile levels after WOS&lt;ref name=ref1/&gt;.<br /> <br /> ===Expression===<br /> <br /> (1)OsJar1 transcripts were up-regulated transiently in response to treatment with exogenous methyl-jasmonic acid (MeJA)&lt;ref name=ref3/&gt;.The expression levels of OsJAR1 and OsJAR2 were induced in response to wounding with the concomitant accumulation of JA-Ile.&lt;ref name=ref2/&gt;<br /> <br /> (2)Real-time PCR was conducted according to the QuantiTect SYBR Green PCR handbook (Qiagen). PCR was perfomed in ABI PRISM 7000 cycler (PE Applied Biosystems, Warrington, UK) using QuantiTect SYBR green-mastermix with HotStar Taq DNA polymerase following the recommended conditions [15 min 95 °C, 40× (15 s, 94 °C; 30 s, 60 °C; 30 s, 72 °C)]. JAR1F2 and JAR1R2 were used as primers to amplify OsJar1 cDNA, Rubg237F and Rubg304R for ubiquitin, which was used as the endogenous control gene.<br /> <br /> For RT-PCR, first-strand cDNA synthesis kit for RT-PCR (Roche, Mannheim, Germany) was performed according to the manufacturer's description. A 1 µL aliquot of a 1:10 dilution of the RT product was used as a template in the subsequent PCR reaction. For amplification of OsJAR1 cDNA, the primers JAR1F1 and JAR1R2 were used, for ubiquitin Rubg237F and Rubg 304R. The PCR conditions were 1 min 94 °C, 30× (94 °C, 1 min; 60 °C, 1 min; 72 °C, 2 min), 72 °C, 7 min.&lt;ref name=ref3/&gt;<br /> <br /> Primers used in real-time PCR and RT-PCR:<br /> <br /> JAR1F1: TAAGGCATTACGCAGGGAAC<br /> <br /> JAR1F2: AGTGCTGAGCATCAACATCG<br /> <br /> JAR1R1: CCAGATAACAGCACCAGAAGC<br /> <br /> JAR1R2: CACCACCAAGGCTTAGGAAA<br /> <br /> Rubg237F: GAGCCTCTGTTCGTCAAGTA<br /> <br /> Rubg304R: ACTCGATGGTCCATTAAACC&lt;ref name=ref3/&gt;<br /> <br /> <br /> ===Evolution===<br /> OsJar1 belongs to the GH3 family in rice. Another member of this family in Arabidopsis, DWARF IN LIGHT 2 (DFL2), showed 47% identities. Dfl2 was shown to be a light-dependent gene, but its gene product did not show enzymatic activity on plant hormones, presumably (Takase et al. 2003). OsJAR1 contained three domains described to be important for the enzymatic function of GH3 proteins (Chang, Xiang &amp; Dunaway-Mariano 1997; Staswick et al. 2002; Terol et al. 2006).&lt;ref name=ref3/&gt;<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> *Biotechnology Research Center, The University of Tokyo, Tokyo 113-8657, Japan.<br /> *Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan<br /> *National Agricultural Research Center of Hokkaido region, National Agriculture and Food Research Organization, Sapporo 062-8555, Japan <br /> *Botanical Institute, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany<br /> *Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena,Germany<br /> *Department of Plant Physiology, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan<br /> *RIKEN Plant Science Center, Tsurumi,Yokohama, Kanagawa 230-0045, Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> * &lt;ref name=&quot;ref1&quot;&gt;<br /> Kaori Fukumoto, Kabir Md Alamgir, Yuko Yamashita, Ivan Galis, et al. (2013) Response of rice to insect elicitors and the role of OsJAR1 in wound and herbivory-induced JA-Ile accumulation[J]. Integr Plant Biol 55: 775–784.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref2&quot;&gt;<br /> Shinji Wakutaa, Erika Suzukia, Wataru Saburia,*, Hideyuki Matsuuraa, Kensuke Nabetaa, Ryozo Imaib and Hirokazu Matsuia (2011) OsJAR1 and OsJAR2 are jasmonyl-L-isoleucine synthases involved in wound- and pathogen-induced jasmonic acid signaling[J].Biochemical and Biophysical Research Communications,409(4): 634-639.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref3&quot;&gt;<br /> MAREN RIEMANN, MICHAEL RIEMANN and MAKOTO TAKANO. (2008) Rice JASMONATE RESISTANT 1 is involved in phytochrome and jasmonate signalling. Plant, Cell &amp; Environment 31(6):783–792.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref4&quot;&gt;<br /> Shimizu T, Miyamoto K, Miyamoto K, Minami E, Nishizawa Y, Iino M, Nojiri H, Yamane H, Okada K. (2013) OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice[J].Bioscience, Biotechnology, and Biochemistry 77(7):1556-1564.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref5&quot;&gt;<br /> KATHARINA SVYATYNA, YUSUKE JIKUMARU, RITA BRENDEL, MICHAEL REICHELT, AXEL MITHÖFER, MAKOTO TAKANO, YUJI KAMIYA, PETER NICK &amp; MICHAEL RIEMANN. (2013) Light induces jasmonate-isoleucine conjugation via OsJAR1-dependent and -independent pathways in rice.Plant, Cell &amp; Environment 37(4):827-839.<br /> &lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0586200|<br /> Description = GH3 auxin-responsive promoter family protein|<br /> Version = NM_001062995.1 GI:115465720 GeneID:4339756|<br /> Length = 5516 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0586200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:29281780..29287295|<br /> CDS = 29284407..29284687,29284793..29284894,29285094..29285911,29286499..29287043|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccgtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MTICSCEETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNF GLGGRTDAESYKSCIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKP KFIPFNDELLETTLQIYRTSYAFRNREYPIGQGKALQFVYGSKQVITKGGILATTATT NLYRRQRYKEGMKDIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAH SLVHAFQTFEEVWEDLCTDIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIG LSNWYGVIPALWPNAKYVYGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNI DPTVPPEQVTYAVLPQVGYFEFIPLEKPIGEETENSASIHYIESDPVGLTEVEVGKIY EVVITNFAGLYRYRLGDVVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVE EASKFLEGEKLEVMDFTSFVERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDA GYTGSRKIKTIGPLELRILRKGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQIL SRNVTQSYFSTAYGF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2628..2908#3014..3115#3315..4132#4720..5264#acccgctctctcctctcatctcctcctcctgtgttcgtgttcttcttcttcttgtagcagcgcttaaccaacccatcattagcgaccaaacctctctccctctccccctctgactaatcaaaattaaacaatttcgatctccaccttcctcttcttgattccagttccaccagagagagacagggtcttctctaatccatgtggtgcttgttggtctcgcttgcttcgtcttcgtcttcgtcttcctcttctcgcacctagtcccgcctcgtcgcctcctccggagaccggagccggacgggacggttgccggcgagcggcggtcgctttcttctctcggccctactcacggtcagtgattggctcccttccttcctggtgttctattgatcctttgtgggggaaaaaaggcttcgatttggggggcatgttcttgcctttttcctgttttgggggagacgagacggatttcaatatatttttttaatctggggggattatgtgtggaaaagattgttcctcgtttatctgggtagtatttggagggtcctatggggatattctttgtaaagctgggatttttaggtggtaaaataatgttgatatactttcagaaagaatatactgcagaaagtttttcagagataacagataagattaggccgtccttaatctcctttctttgcgtctgttccctccaactaggatggaaaagaatggcggattgattagtcagaaactggctgctgaatactctgagatttcatttcatttcagcctctgttgattcctcatggctgtctctaacttctttgcttgtgcttccgatttctttggcggtaaatagcctccacgaatctctggtcaacgaggtattaaaacttaaaagggcttttgtctcaggttgacccgagccatgattcgcccccctcccatgctttgcaaacaagatatgatacgcagtgctggtacaattcagagcatatgttctttcctcttttgctttgtttcagacagcaacttttagcagccacgcctctcacaagttgataaaaaaaaattttgtgttgctctcctactactcatcactttctagtgcgatatagtagtagtagtagtaggagtatatgctagcactatccacagtttagcacacagattggtggcacctgggcagatataaaagcttcacatttttatcaatgtctgcgcagttatattgtcgatttgattccttttatagatattactaggtcaagtcgtgtcgcaattactccccccctttcctccattgtagggtgttcctgtttagtccgcgggggatgctgctccctgagcctgtgtgtgtctcttgatttttctttctttgctcgcacgcagcagcagcagcatgttttgtgtcggtgttaaggaccctacataaataattcatcaaatcaagtatcttttgtctgttttggggaatctcctttgcggttcgtccaaaatttcgcgaaagaaacaattctatatgactgcttttgtcgagaggttagacatcactgctagagtagtagagcaatgctacacgtactaaatttttcttactaaacttttactaataatcatctcaaccgtttgatttaagtagatggaatttacaaaaaatctagatgcactcgtagcatgacacatcagtgttagtaagaatttagtaagaaaaagttagtacgtatagccttttccagagtagtactagcagagtgttagattgtatcacctgctcgggcagttgtaaatggtatggtgagttggtgactggtttttacagttttttcatacttgggtttatggaggatttactgttatggagacctgtaaaaaatgttacatatattgctactttctcatttggatttcggatggctgttttttttatcattatgttggtaaagttttgcatcaagaatctgaaccagcttagcaatactaatagtagatggttcagtaagctgcttccatgtccttgcaactttgcaatgctggcaattttttttcgaaaaagagcaaggctggcaatatgtgtatagcttttgtttggcaatatatgttgcgattctagcgctggtggtatctcaagatcagaaaaagtgagcatcaatgtcttactttattggttttctgactgattacattagtaaacgctgtgtccaaaagtatagagtaatactagttatattttcttaattgtcagtgactcagtatccgcagatggtttgtaaagtgcccttatttttcatggtcgttagctttagtaggtatatagtgtactcttgatggtttatcctttttttcgcccctttctcctttgtcatctgggcgcacgttgctatgcagtttcatgagatggttagtgcgtatccaactattctgtcggcaataaagttccacaatcttcaaaattttctaccattctgatgccaactcctactgcactatcagctcttggtttccagctcagttagtggttagaaattgcagcctacatgaaaaagtaactaatagattttctcgtctccaccttttatagttattttcagtgactcttcttccaaaccaagtggtaaagccaggattctaccattacaatgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcaggtactgtacccattgcttgaaatttgtcaccttattatgcttatcgtttactagtttatgataataaatgttcatagtctggcttcttctgtggctttcatacagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccggtaaggatctctgatttattattatttatgctcaagtaatgctgctttttctgaatgttcaacaaactttgatgatcaggttcaacatgagttattgtttttatacactgttttgcatgctattaacttgttgcttctttgtgtattttttactctgcatcccagaatattagaataaccttctgttttgtgtttacagtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtatattctctcttttctatctattctgaatcttaaattgggttaaatctccattatctagaagtatagcaatgaaaattctacaaaaagcaaggggcagccatccctagttctgcttctggtgctgttatctggtcagacatgataccaacgggatttatcagaaataactagataagtttgtgtgacatattatttcaaaaaagaaatatttattagtcttgccccttcaatcttaaacctctttttgacaagagctccttattatgtaaaaaacataaaaatcacacggttgcaatgtatacatcaatattattaagcacatgggggagtaattattgtgaatgtttgtggtccactccttgaactctttgctgcttactcattatgattatcttaaaactctatcagatgatatatgtttctttttgtgtatgccaaccatgccagactcaccaataacatttttattctttaatatctctggctaacatacaattattttgttttaggacacagaaagtttacttattacggtgatgtttaagaaacactaaaatctttttcttttttctattttgttctaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttgatacaaagaaacatattagatgctagcagctgcatgtaattacctttttacctcttgtttttcagctttactgtagtctccaccggaagaaaacgtttggtttagtctcttgaataatctattttcttatcctgtttgtgactatctagaatctatgtaaatggcccagctctagtgaaagttatggtgatgttatcaggaaagaaaatgtggtgtttcccgtaacaagtgctgcaaagtattattcagactc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062995.1 RefSeq:Os05g0586200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0586200&diff=169570 2014-05-18T18:34:24Z

<p>Shixing: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information<br /> haha<br /> ==Annotated Information==<br /> ===Function===<br /> [[File:Expression.figure1.png|thumb|right|'''Figure1:'''''Gene expression and hormone content in wild type and OsJAR1 TOS17 insertion lines after simulated herbivory (WOS)(frome reference&lt;ref name=ref1/&gt;).'']]<br /> [[File:Expression.figure2.png|thumb|right|'''Figure2:'''''The expression levels of OsJAR1 and OsJAR2 mRNA and the accumulation of JA and JA-amino acid conjugates in response to wounding(from reference&lt;ref name=ref2/&gt;).'']]<br /> OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice by the use of an osjar1 Tos17 mutant.&lt;ref name=ref1/&gt;&lt;ref name=ref4/&gt;It is involved in phytochrome and jasmonate signalling. In far-red and blue lights, osjar1 coleoptiles were longer if compared with the wild type (WT), indicating that OsJar1participates in the suppression of coleoptile elongation in these light conditions, while the mutant did not show a clear phenotype in red light.OsJar1 modulates light and JA signalling in the photomorphogenesis of rice.&lt;ref name=ref3/&gt; Light can induce jasmonate-isoleucine conjugation via OsJAR1-dependent pathways in rice. OsJAR1 is the most important JA-Ile conjugating enzyme in the wounding response during the vegetative stage.&lt;ref name=ref5/&gt;Plants produce jasmonic acid (JA) and its amino acid conjugate, jasmonoyl‐L‐isoleucine (JA‐Ile) as major defense signals in response to wounding and herbivory. OsJAR1‐deficient plants have strongly reduced JA‐Ile levels after WOS&lt;ref name=ref1/&gt;.<br /> <br /> ===Expression===<br /> <br /> (1)OsJar1 transcripts were up-regulated transiently in response to treatment with exogenous methyl-jasmonic acid (MeJA)&lt;ref name=ref3/&gt;.The expression levels of OsJAR1 and OsJAR2 were induced in response to wounding with the concomitant accumulation of JA-Ile.&lt;ref name=ref2/&gt;<br /> <br /> (2)Real-time PCR was conducted according to the QuantiTect SYBR Green PCR handbook (Qiagen). PCR was perfomed in ABI PRISM 7000 cycler (PE Applied Biosystems, Warrington, UK) using QuantiTect SYBR green-mastermix with HotStar Taq DNA polymerase following the recommended conditions [15 min 95 °C, 40× (15 s, 94 °C; 30 s, 60 °C; 30 s, 72 °C)]. JAR1F2 and JAR1R2 were used as primers to amplify OsJar1 cDNA, Rubg237F and Rubg304R for ubiquitin, which was used as the endogenous control gene.<br /> <br /> For RT-PCR, first-strand cDNA synthesis kit for RT-PCR (Roche, Mannheim, Germany) was performed according to the manufacturer's description. A 1 µL aliquot of a 1:10 dilution of the RT product was used as a template in the subsequent PCR reaction. For amplification of OsJAR1 cDNA, the primers JAR1F1 and JAR1R2 were used, for ubiquitin Rubg237F and Rubg 304R. The PCR conditions were 1 min 94 °C, 30× (94 °C, 1 min; 60 °C, 1 min; 72 °C, 2 min), 72 °C, 7 min.&lt;ref name=ref3/&gt;<br /> <br /> Primers used in real-time PCR and RT-PCR:<br /> <br /> JAR1F1: TAAGGCATTACGCAGGGAAC<br /> <br /> JAR1F2: AGTGCTGAGCATCAACATCG<br /> <br /> JAR1R1: CCAGATAACAGCACCAGAAGC<br /> <br /> JAR1R2: CACCACCAAGGCTTAGGAAA<br /> <br /> Rubg237F: GAGCCTCTGTTCGTCAAGTA<br /> <br /> Rubg304R: ACTCGATGGTCCATTAAACC&lt;ref name=ref3/&gt;<br /> <br /> <br /> ===Evolution===<br /> OsJar1 belongs to the GH3 family in rice. Another member of this family in Arabidopsis, DWARF IN LIGHT 2 (DFL2), showed 47% identities. Dfl2 was shown to be a light-dependent gene, but its gene product did not show enzymatic activity on plant hormones, presumably (Takase et al. 2003). OsJAR1 contained three domains described to be important for the enzymatic function of GH3 proteins (Chang, Xiang &amp; Dunaway-Mariano 1997; Staswick et al. 2002; Terol et al. 2006).&lt;ref name=ref3/&gt;<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> *Biotechnology Research Center, The University of Tokyo, Tokyo 113-8657, Japan.<br /> *Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan<br /> *National Agricultural Research Center of Hokkaido region, National Agriculture and Food Research Organization, Sapporo 062-8555, Japan <br /> *Botanical Institute, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany<br /> *Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena,Germany<br /> *Department of Plant Physiology, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan<br /> *RIKEN Plant Science Center, Tsurumi,Yokohama, Kanagawa 230-0045, Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> * &lt;ref name=&quot;ref1&quot;&gt;<br /> Kaori Fukumoto, Kabir Md Alamgir, Yuko Yamashita, Ivan Galis, et al. (2013) Response of rice to insect elicitors and the role of OsJAR1 in wound and herbivory-induced JA-Ile accumulation[J]. Integr Plant Biol 55: 775–784.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref2&quot;&gt;<br /> Shinji Wakutaa, Erika Suzukia, Wataru Saburia,*, Hideyuki Matsuuraa, Kensuke Nabetaa, Ryozo Imaib and Hirokazu Matsuia (2011) OsJAR1 and OsJAR2 are jasmonyl-L-isoleucine synthases involved in wound- and pathogen-induced jasmonic acid signaling[J].Biochemical and Biophysical Research Communications,409(4): 634-639.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref3&quot;&gt;<br /> MAREN RIEMANN, MICHAEL RIEMANN and MAKOTO TAKANO. (2008) Rice JASMONATE RESISTANT 1 is involved in phytochrome and jasmonate signalling. Plant, Cell &amp; Environment 31(6):783–792.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref4&quot;&gt;<br /> Shimizu T, Miyamoto K, Miyamoto K, Minami E, Nishizawa Y, Iino M, Nojiri H, Yamane H, Okada K. (2013) OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice[J].Bioscience, Biotechnology, and Biochemistry 77(7):1556-1564.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref5&quot;&gt;<br /> KATHARINA SVYATYNA, YUSUKE JIKUMARU, RITA BRENDEL, MICHAEL REICHELT, AXEL MITHÖFER, MAKOTO TAKANO, YUJI KAMIYA, PETER NICK &amp; MICHAEL RIEMANN. (2013) Light induces jasmonate-isoleucine conjugation via OsJAR1-dependent and -independent pathways in rice.Plant, Cell &amp; Environment 37(4):827-839.<br /> &lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0586200|<br /> Description = GH3 auxin-responsive promoter family protein|<br /> Version = NM_001062995.1 GI:115465720 GeneID:4339756|<br /> Length = 5516 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0586200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:29281780..29287295|<br /> CDS = 29284407..29284687,29284793..29284894,29285094..29285911,29286499..29287043|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccgtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MTICSCEETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNF GLGGRTDAESYKSCIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKP KFIPFNDELLETTLQIYRTSYAFRNREYPIGQGKALQFVYGSKQVITKGGILATTATT NLYRRQRYKEGMKDIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAH SLVHAFQTFEEVWEDLCTDIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIG LSNWYGVIPALWPNAKYVYGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNI DPTVPPEQVTYAVLPQVGYFEFIPLEKPIGEETENSASIHYIESDPVGLTEVEVGKIY EVVITNFAGLYRYRLGDVVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVE EASKFLEGEKLEVMDFTSFVERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDA GYTGSRKIKTIGPLELRILRKGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQIL SRNVTQSYFSTAYGF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2628..2908#3014..3115#3315..4132#4720..5264#acccgctctctcctctcatctcctcctcctgtgttcgtgttcttcttcttcttgtagcagcgcttaaccaacccatcattagcgaccaaacctctctccctctccccctctgactaatcaaaattaaacaatttcgatctccaccttcctcttcttgattccagttccaccagagagagacagggtcttctctaatccatgtggtgcttgttggtctcgcttgcttcgtcttcgtcttcgtcttcctcttctcgcacctagtcccgcctcgtcgcctcctccggagaccggagccggacgggacggttgccggcgagcggcggtcgctttcttctctcggccctactcacggtcagtgattggctcccttccttcctggtgttctattgatcctttgtgggggaaaaaaggcttcgatttggggggcatgttcttgcctttttcctgttttgggggagacgagacggatttcaatatatttttttaatctggggggattatgtgtggaaaagattgttcctcgtttatctgggtagtatttggagggtcctatggggatattctttgtaaagctgggatttttaggtggtaaaataatgttgatatactttcagaaagaatatactgcagaaagtttttcagagataacagataagattaggccgtccttaatctcctttctttgcgtctgttccctccaactaggatggaaaagaatggcggattgattagtcagaaactggctgctgaatactctgagatttcatttcatttcagcctctgttgattcctcatggctgtctctaacttctttgcttgtgcttccgatttctttggcggtaaatagcctccacgaatctctggtcaacgaggtattaaaacttaaaagggcttttgtctcaggttgacccgagccatgattcgcccccctcccatgctttgcaaacaagatatgatacgcagtgctggtacaattcagagcatatgttctttcctcttttgctttgtttcagacagcaacttttagcagccacgcctctcacaagttgataaaaaaaaattttgtgttgctctcctactactcatcactttctagtgcgatatagtagtagtagtagtaggagtatatgctagcactatccacagtttagcacacagattggtggcacctgggcagatataaaagcttcacatttttatcaatgtctgcgcagttatattgtcgatttgattccttttatagatattactaggtcaagtcgtgtcgcaattactccccccctttcctccattgtagggtgttcctgtttagtccgcgggggatgctgctccctgagcctgtgtgtgtctcttgatttttctttctttgctcgcacgcagcagcagcagcatgttttgtgtcggtgttaaggaccctacataaataattcatcaaatcaagtatcttttgtctgttttggggaatctcctttgcggttcgtccaaaatttcgcgaaagaaacaattctatatgactgcttttgtcgagaggttagacatcactgctagagtagtagagcaatgctacacgtactaaatttttcttactaaacttttactaataatcatctcaaccgtttgatttaagtagatggaatttacaaaaaatctagatgcactcgtagcatgacacatcagtgttagtaagaatttagtaagaaaaagttagtacgtatagccttttccagagtagtactagcagagtgttagattgtatcacctgctcgggcagttgtaaatggtatggtgagttggtgactggtttttacagttttttcatacttgggtttatggaggatttactgttatggagacctgtaaaaaatgttacatatattgctactttctcatttggatttcggatggctgttttttttatcattatgttggtaaagttttgcatcaagaatctgaaccagcttagcaatactaatagtagatggttcagtaagctgcttccatgtccttgcaactttgcaatgctggcaattttttttcgaaaaagagcaaggctggcaatatgtgtatagcttttgtttggcaatatatgttgcgattctagcgctggtggtatctcaagatcagaaaaagtgagcatcaatgtcttactttattggttttctgactgattacattagtaaacgctgtgtccaaaagtatagagtaatactagttatattttcttaattgtcagtgactcagtatccgcagatggtttgtaaagtgcccttatttttcatggtcgttagctttagtaggtatatagtgtactcttgatggtttatcctttttttcgcccctttctcctttgtcatctgggcgcacgttgctatgcagtttcatgagatggttagtgcgtatccaactattctgtcggcaataaagttccacaatcttcaaaattttctaccattctgatgccaactcctactgcactatcagctcttggtttccagctcagttagtggttagaaattgcagcctacatgaaaaagtaactaatagattttctcgtctccaccttttatagttattttcagtgactcttcttccaaaccaagtggtaaagccaggattctaccattacaatgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcaggtactgtacccattgcttgaaatttgtcaccttattatgcttatcgtttactagtttatgataataaatgttcatagtctggcttcttctgtggctttcatacagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccggtaaggatctctgatttattattatttatgctcaagtaatgctgctttttctgaatgttcaacaaactttgatgatcaggttcaacatgagttattgtttttatacactgttttgcatgctattaacttgttgcttctttgtgtattttttactctgcatcccagaatattagaataaccttctgttttgtgtttacagtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtatattctctcttttctatctattctgaatcttaaattgggttaaatctccattatctagaagtatagcaatgaaaattctacaaaaagcaaggggcagccatccctagttctgcttctggtgctgttatctggtcagacatgataccaacgggatttatcagaaataactagataagtttgtgtgacatattatttcaaaaaagaaatatttattagtcttgccccttcaatcttaaacctctttttgacaagagctccttattatgtaaaaaacataaaaatcacacggttgcaatgtatacatcaatattattaagcacatgggggagtaattattgtgaatgtttgtggtccactccttgaactctttgctgcttactcattatgattatcttaaaactctatcagatgatatatgtttctttttgtgtatgccaaccatgccagactcaccaataacatttttattctttaatatctctggctaacatacaattattttgttttaggacacagaaagtttacttattacggtgatgtttaagaaacactaaaatctttttcttttttctattttgttctaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttgatacaaagaaacatattagatgctagcagctgcatgtaattacctttttacctcttgtttttcagctttactgtagtctccaccggaagaaaacgtttggtttagtctcttgaataatctattttcttatcctgtttgtgactatctagaatctatgtaaatggcccagctctagtgaaagttatggtgatgttatcaggaaagaaaatgtggtgtttcccgtaacaagtgctgcaaagtattattcagactc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062995.1 RefSeq:Os05g0586200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0586200&diff=169569 2014-05-18T18:32:18Z

<p>Shixing: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information<br /> ==Annotated Information==<br /> ===Function===<br /> [[File:Expression.figure1.png|thumb|right|'''Figure1:'''''Gene expression and hormone content in wild type and OsJAR1 TOS17 insertion lines after simulated herbivory (WOS)(frome reference&lt;ref name=ref1/&gt;).'']]<br /> [[File:Expression.figure2.png|thumb|right|'''Figure2:'''''The expression levels of OsJAR1 and OsJAR2 mRNA and the accumulation of JA and JA-amino acid conjugates in response to wounding(from reference&lt;ref name=ref2/&gt;).'']]<br /> OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice by the use of an osjar1 Tos17 mutant.&lt;ref name=ref1/&gt;&lt;ref name=ref4/&gt;It is involved in phytochrome and jasmonate signalling. In far-red and blue lights, osjar1 coleoptiles were longer if compared with the wild type (WT), indicating that OsJar1participates in the suppression of coleoptile elongation in these light conditions, while the mutant did not show a clear phenotype in red light.OsJar1 modulates light and JA signalling in the photomorphogenesis of rice.&lt;ref name=ref3/&gt; Light can induce jasmonate-isoleucine conjugation via OsJAR1-dependent pathways in rice. OsJAR1 is the most important JA-Ile conjugating enzyme in the wounding response during the vegetative stage.&lt;ref name=ref5/&gt;Plants produce jasmonic acid (JA) and its amino acid conjugate, jasmonoyl‐L‐isoleucine (JA‐Ile) as major defense signals in response to wounding and herbivory. OsJAR1‐deficient plants have strongly reduced JA‐Ile levels after WOS&lt;ref name=ref1/&gt;.<br /> <br /> ===Expression===<br /> <br /> (1)OsJar1 transcripts were up-regulated transiently in response to treatment with exogenous methyl-jasmonic acid (MeJA)&lt;ref name=ref3/&gt;.The expression levels of OsJAR1 and OsJAR2 were induced in response to wounding with the concomitant accumulation of JA-Ile.&lt;ref name=ref2/&gt;<br /> <br /> (2)Real-time PCR was conducted according to the QuantiTect SYBR Green PCR handbook (Qiagen). PCR was perfomed in ABI PRISM 7000 cycler (PE Applied Biosystems, Warrington, UK) using QuantiTect SYBR green-mastermix with HotStar Taq DNA polymerase following the recommended conditions [15 min 95 °C, 40× (15 s, 94 °C; 30 s, 60 °C; 30 s, 72 °C)]. JAR1F2 and JAR1R2 were used as primers to amplify OsJar1 cDNA, Rubg237F and Rubg304R for ubiquitin, which was used as the endogenous control gene.<br /> <br /> For RT-PCR, first-strand cDNA synthesis kit for RT-PCR (Roche, Mannheim, Germany) was performed according to the manufacturer's description. A 1 µL aliquot of a 1:10 dilution of the RT product was used as a template in the subsequent PCR reaction. For amplification of OsJAR1 cDNA, the primers JAR1F1 and JAR1R2 were used, for ubiquitin Rubg237F and Rubg 304R. The PCR conditions were 1 min 94 °C, 30× (94 °C, 1 min; 60 °C, 1 min; 72 °C, 2 min), 72 °C, 7 min.&lt;ref name=ref3/&gt;<br /> <br /> Primers used in real-time PCR and RT-PCR:<br /> <br /> JAR1F1: TAAGGCATTACGCAGGGAAC<br /> <br /> JAR1F2: AGTGCTGAGCATCAACATCG<br /> <br /> JAR1R1: CCAGATAACAGCACCAGAAGC<br /> <br /> JAR1R2: CACCACCAAGGCTTAGGAAA<br /> <br /> Rubg237F: GAGCCTCTGTTCGTCAAGTA<br /> <br /> Rubg304R: ACTCGATGGTCCATTAAACC&lt;ref name=ref3/&gt;<br /> <br /> <br /> ===Evolution===<br /> OsJar1 belongs to the GH3 family in rice. Another member of this family in Arabidopsis, DWARF IN LIGHT 2 (DFL2), showed 47% identities. Dfl2 was shown to be a light-dependent gene, but its gene product did not show enzymatic activity on plant hormones, presumably (Takase et al. 2003). OsJAR1 contained three domains described to be important for the enzymatic function of GH3 proteins (Chang, Xiang &amp; Dunaway-Mariano 1997; Staswick et al. 2002; Terol et al. 2006).&lt;ref name=ref3/&gt;<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> *Biotechnology Research Center, The University of Tokyo, Tokyo 113-8657, Japan.<br /> *Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan<br /> *National Agricultural Research Center of Hokkaido region, National Agriculture and Food Research Organization, Sapporo 062-8555, Japan <br /> *Botanical Institute, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany<br /> *Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena,Germany<br /> *Department of Plant Physiology, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan<br /> *RIKEN Plant Science Center, Tsurumi,Yokohama, Kanagawa 230-0045, Japan<br /> <br /> ==References==<br /> &lt;references&gt;<br /> * &lt;ref name=&quot;ref1&quot;&gt;<br /> Kaori Fukumoto, Kabir Md Alamgir, Yuko Yamashita, Ivan Galis, et al. (2013) Response of rice to insect elicitors and the role of OsJAR1 in wound and herbivory-induced JA-Ile accumulation[J]. Integr Plant Biol 55: 775–784.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref2&quot;&gt;<br /> Shinji Wakutaa, Erika Suzukia, Wataru Saburia,*, Hideyuki Matsuuraa, Kensuke Nabetaa, Ryozo Imaib and Hirokazu Matsuia (2011) OsJAR1 and OsJAR2 are jasmonyl-L-isoleucine synthases involved in wound- and pathogen-induced jasmonic acid signaling[J].Biochemical and Biophysical Research Communications,409(4): 634-639.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref3&quot;&gt;<br /> MAREN RIEMANN, MICHAEL RIEMANN and MAKOTO TAKANO. (2008) Rice JASMONATE RESISTANT 1 is involved in phytochrome and jasmonate signalling. Plant, Cell &amp; Environment 31(6):783–792.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref4&quot;&gt;<br /> Shimizu T, Miyamoto K, Miyamoto K, Minami E, Nishizawa Y, Iino M, Nojiri H, Yamane H, Okada K. (2013) OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice[J].Bioscience, Biotechnology, and Biochemistry 77(7):1556-1564.<br /> &lt;/ref&gt;<br /> * &lt;ref name=&quot;ref5&quot;&gt;<br /> KATHARINA SVYATYNA, YUSUKE JIKUMARU, RITA BRENDEL, MICHAEL REICHELT, AXEL MITHÖFER, MAKOTO TAKANO, YUJI KAMIYA, PETER NICK &amp; MICHAEL RIEMANN. (2013) Light induces jasmonate-isoleucine conjugation via OsJAR1-dependent and -independent pathways in rice.Plant, Cell &amp; Environment 37(4):827-839.<br /> &lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0586200|<br /> Description = GH3 auxin-responsive promoter family protein|<br /> Version = NM_001062995.1 GI:115465720 GeneID:4339756|<br /> Length = 5516 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0586200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:29281780..29287295|<br /> CDS = 29284407..29284687,29284793..29284894,29285094..29285911,29286499..29287043|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccgtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MTICSCEETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNF GLGGRTDAESYKSCIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKP KFIPFNDELLETTLQIYRTSYAFRNREYPIGQGKALQFVYGSKQVITKGGILATTATT NLYRRQRYKEGMKDIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAH SLVHAFQTFEEVWEDLCTDIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIG LSNWYGVIPALWPNAKYVYGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNI DPTVPPEQVTYAVLPQVGYFEFIPLEKPIGEETENSASIHYIESDPVGLTEVEVGKIY EVVITNFAGLYRYRLGDVVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVE EASKFLEGEKLEVMDFTSFVERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDA GYTGSRKIKTIGPLELRILRKGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQIL SRNVTQSYFSTAYGF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2628..2908#3014..3115#3315..4132#4720..5264#acccgctctctcctctcatctcctcctcctgtgttcgtgttcttcttcttcttgtagcagcgcttaaccaacccatcattagcgaccaaacctctctccctctccccctctgactaatcaaaattaaacaatttcgatctccaccttcctcttcttgattccagttccaccagagagagacagggtcttctctaatccatgtggtgcttgttggtctcgcttgcttcgtcttcgtcttcgtcttcctcttctcgcacctagtcccgcctcgtcgcctcctccggagaccggagccggacgggacggttgccggcgagcggcggtcgctttcttctctcggccctactcacggtcagtgattggctcccttccttcctggtgttctattgatcctttgtgggggaaaaaaggcttcgatttggggggcatgttcttgcctttttcctgttttgggggagacgagacggatttcaatatatttttttaatctggggggattatgtgtggaaaagattgttcctcgtttatctgggtagtatttggagggtcctatggggatattctttgtaaagctgggatttttaggtggtaaaataatgttgatatactttcagaaagaatatactgcagaaagtttttcagagataacagataagattaggccgtccttaatctcctttctttgcgtctgttccctccaactaggatggaaaagaatggcggattgattagtcagaaactggctgctgaatactctgagatttcatttcatttcagcctctgttgattcctcatggctgtctctaacttctttgcttgtgcttccgatttctttggcggtaaatagcctccacgaatctctggtcaacgaggtattaaaacttaaaagggcttttgtctcaggttgacccgagccatgattcgcccccctcccatgctttgcaaacaagatatgatacgcagtgctggtacaattcagagcatatgttctttcctcttttgctttgtttcagacagcaacttttagcagccacgcctctcacaagttgataaaaaaaaattttgtgttgctctcctactactcatcactttctagtgcgatatagtagtagtagtagtaggagtatatgctagcactatccacagtttagcacacagattggtggcacctgggcagatataaaagcttcacatttttatcaatgtctgcgcagttatattgtcgatttgattccttttatagatattactaggtcaagtcgtgtcgcaattactccccccctttcctccattgtagggtgttcctgtttagtccgcgggggatgctgctccctgagcctgtgtgtgtctcttgatttttctttctttgctcgcacgcagcagcagcagcatgttttgtgtcggtgttaaggaccctacataaataattcatcaaatcaagtatcttttgtctgttttggggaatctcctttgcggttcgtccaaaatttcgcgaaagaaacaattctatatgactgcttttgtcgagaggttagacatcactgctagagtagtagagcaatgctacacgtactaaatttttcttactaaacttttactaataatcatctcaaccgtttgatttaagtagatggaatttacaaaaaatctagatgcactcgtagcatgacacatcagtgttagtaagaatttagtaagaaaaagttagtacgtatagccttttccagagtagtactagcagagtgttagattgtatcacctgctcgggcagttgtaaatggtatggtgagttggtgactggtttttacagttttttcatacttgggtttatggaggatttactgttatggagacctgtaaaaaatgttacatatattgctactttctcatttggatttcggatggctgttttttttatcattatgttggtaaagttttgcatcaagaatctgaaccagcttagcaatactaatagtagatggttcagtaagctgcttccatgtccttgcaactttgcaatgctggcaattttttttcgaaaaagagcaaggctggcaatatgtgtatagcttttgtttggcaatatatgttgcgattctagcgctggtggtatctcaagatcagaaaaagtgagcatcaatgtcttactttattggttttctgactgattacattagtaaacgctgtgtccaaaagtatagagtaatactagttatattttcttaattgtcagtgactcagtatccgcagatggtttgtaaagtgcccttatttttcatggtcgttagctttagtaggtatatagtgtactcttgatggtttatcctttttttcgcccctttctcctttgtcatctgggcgcacgttgctatgcagtttcatgagatggttagtgcgtatccaactattctgtcggcaataaagttccacaatcttcaaaattttctaccattctgatgccaactcctactgcactatcagctcttggtttccagctcagttagtggttagaaattgcagcctacatgaaaaagtaactaatagattttctcgtctccaccttttatagttattttcagtgactcttcttccaaaccaagtggtaaagccaggattctaccattacaatgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcaggtactgtacccattgcttgaaatttgtcaccttattatgcttatcgtttactagtttatgataataaatgttcatagtctggcttcttctgtggctttcatacagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccggtaaggatctctgatttattattatttatgctcaagtaatgctgctttttctgaatgttcaacaaactttgatgatcaggttcaacatgagttattgtttttatacactgttttgcatgctattaacttgttgcttctttgtgtattttttactctgcatcccagaatattagaataaccttctgttttgtgtttacagtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtatattctctcttttctatctattctgaatcttaaattgggttaaatctccattatctagaagtatagcaatgaaaattctacaaaaagcaaggggcagccatccctagttctgcttctggtgctgttatctggtcagacatgataccaacgggatttatcagaaataactagataagtttgtgtgacatattatttcaaaaaagaaatatttattagtcttgccccttcaatcttaaacctctttttgacaagagctccttattatgtaaaaaacataaaaatcacacggttgcaatgtatacatcaatattattaagcacatgggggagtaattattgtgaatgtttgtggtccactccttgaactctttgctgcttactcattatgattatcttaaaactctatcagatgatatatgtttctttttgtgtatgccaaccatgccagactcaccaataacatttttattctttaatatctctggctaacatacaattattttgttttaggacacagaaagtttacttattacggtgatgtttaagaaacactaaaatctttttcttttttctattttgttctaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttgatacaaagaaacatattagatgctagcagctgcatgtaattacctttttacctcttgtttttcagctttactgtagtctccaccggaagaaaacgtttggtttagtctcttgaataatctattttcttatcctgtttgtgactatctagaatctatgtaaatggcccagctctagtgaaagttatggtgatgttatcaggaaagaaaatgtggtgtttcccgtaacaagtgctgcaaagtattattcagactc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062995.1 RefSeq:Os05g0586200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169568 2014-05-18T18:14:06Z

<p>Shixing: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 is a basic helix-loop-helix protein, which interacts with OsJAZ proteins in a jasmonate signalling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signalling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signalling module in the upstream signalling pathway to improve drought tolerance in rice&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> <br /> As figure1 shows, OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding&lt;ref name=&quot;ref1&quot; /&gt;。[[File:Expression.gif|thumb|right|''Figure1: OsDREB148 was rapidly induced by 15 min of exposure to MeJA and was gradually responsive to drought conditions（from reference &lt;ref name=&quot;ref1&quot; /&gt;）.'']].<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> *Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> *Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> *GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> *School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Ju-Seok Seo1, Joungsu Joo2, Min-Jeong Kim1, Yang Do Choi1 et al.(2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.The Plant Journal<br /> Volume 65, Issue 6:907–921.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0586200&diff=169567 2014-05-18T18:13:32Z

<p>Shixing: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information<br /> ==Annotated Information==<br /> ===Function===<br /> [[File:Expression.figure1.png|thumb|right|'''Figure1:'''''Gene expression and hormone content in wild type and OsJAR1 TOS17 insertion lines after simulated herbivory (WOS)(frome reference&lt;ref name=ref1/&gt;).'']]<br /> [[File:Expression.figure2.png|thumb|right|'''Figure2:'''''The expression levels of OsJAR1 and OsJAR2 mRNA and the accumulation of JA and JA-amino acid conjugates in response to wounding(from reference&lt;ref name=ref2/&gt;).'']]<br /> OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice by the use of an osjar1 Tos17 mutant.&lt;ref name=ref1/&gt;&lt;ref name=ref4/&gt;It is involved in phytochrome and jasmonate signalling. In far-red and blue lights, osjar1 coleoptiles were longer if compared with the wild type (WT), indicating that OsJar1participates in the suppression of coleoptile elongation in these light conditions, while the mutant did not show a clear phenotype in red light.OsJar1 modulates light and JA signalling in the photomorphogenesis of rice.&lt;ref name=ref3/&gt; Light can induce jasmonate-isoleucine conjugation via OsJAR1-dependent pathways in rice. OsJAR1 is the most important JA-Ile conjugating enzyme in the wounding response during the vegetative stage.&lt;ref name=ref5/&gt;Plants produce jasmonic acid (JA) and its amino acid conjugate, jasmonoyl‐L‐isoleucine (JA‐Ile) as major defense signals in response to wounding and herbivory. OsJAR1‐deficient plants have strongly reduced JA‐Ile levels after WOS&lt;ref name=ref1/&gt;.<br /> <br /> ===Expression===<br /> <br /> (1)OsJar1 transcripts were up-regulated transiently in response to treatment with exogenous methyl-jasmonic acid (MeJA)&lt;ref name=ref3/&gt;.The expression levels of OsJAR1 and OsJAR2 were induced in response to wounding with the concomitant accumulation of JA-Ile.&lt;ref name=ref2/&gt;<br /> <br /> (2)Real-time PCR was conducted according to the QuantiTect SYBR Green PCR handbook (Qiagen). PCR was perfomed in ABI PRISM 7000 cycler (PE Applied Biosystems, Warrington, UK) using QuantiTect SYBR green-mastermix with HotStar Taq DNA polymerase following the recommended conditions [15 min 95 °C, 40× (15 s, 94 °C; 30 s, 60 °C; 30 s, 72 °C)]. JAR1F2 and JAR1R2 were used as primers to amplify OsJar1 cDNA, Rubg237F and Rubg304R for ubiquitin, which was used as the endogenous control gene.<br /> <br /> For RT-PCR, first-strand cDNA synthesis kit for RT-PCR (Roche, Mannheim, Germany) was performed according to the manufacturer's description. A 1 µL aliquot of a 1:10 dilution of the RT product was used as a template in the subsequent PCR reaction. For amplification of OsJAR1 cDNA, the primers JAR1F1 and JAR1R2 were used, for ubiquitin Rubg237F and Rubg 304R. The PCR conditions were 1 min 94 °C, 30× (94 °C, 1 min; 60 °C, 1 min; 72 °C, 2 min), 72 °C, 7 min.&lt;ref name=ref3/&gt;<br /> <br /> Primers used in real-time PCR and RT-PCR:<br /> <br /> JAR1F1: TAAGGCATTACGCAGGGAAC<br /> <br /> JAR1F2: AGTGCTGAGCATCAACATCG<br /> <br /> JAR1R1: CCAGATAACAGCACCAGAAGC<br /> <br /> JAR1R2: CACCACCAAGGCTTAGGAAA<br /> <br /> Rubg237F: GAGCCTCTGTTCGTCAAGTA<br /> <br /> Rubg304R: ACTCGATGGTCCATTAAACC&lt;ref name=ref3/&gt;<br /> <br /> <br /> ===Evolution===<br /> OsJar1 belongs to the GH3 family in rice. Another member of this family in Arabidopsis, DWARF IN LIGHT 2 (DFL2), showed 47% identities. Dfl2 was shown to be a light-dependent gene, but its gene product did not show enzymatic activity on plant hormones, presumably (Takase et al. 2003). OsJAR1 contained three domains described to be important for the enzymatic function of GH3 proteins (Chang, Xiang &amp; Dunaway-Mariano 1997; Staswick et al. 2002; Terol et al. 2006).&lt;ref name=ref3/&gt;<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> *Biotechnology Research Center, The University of Tokyo, Tokyo 113-8657, Japan.<br /> *Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan<br /> *National Agricultural Research Center of Hokkaido region, National Agriculture and Food Research Organization, Sapporo 062-8555, Japan <br /> *Botanical Institute, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany<br /> *Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena,Germany<br /> *Department of Plant Physiology, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan<br /> *RIKEN Plant Science Center, Tsurumi,Yokohama, Kanagawa 230-0045, Japan<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0586200|<br /> Description = GH3 auxin-responsive promoter family protein|<br /> Version = NM_001062995.1 GI:115465720 GeneID:4339756|<br /> Length = 5516 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0586200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:29281780..29287295|<br /> CDS = 29284407..29284687,29284793..29284894,29285094..29285911,29286499..29287043|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccgtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MTICSCEETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNF GLGGRTDAESYKSCIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKP KFIPFNDELLETTLQIYRTSYAFRNREYPIGQGKALQFVYGSKQVITKGGILATTATT NLYRRQRYKEGMKDIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAH SLVHAFQTFEEVWEDLCTDIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIG LSNWYGVIPALWPNAKYVYGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNI DPTVPPEQVTYAVLPQVGYFEFIPLEKPIGEETENSASIHYIESDPVGLTEVEVGKIY EVVITNFAGLYRYRLGDVVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVE EASKFLEGEKLEVMDFTSFVERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDA GYTGSRKIKTIGPLELRILRKGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQIL SRNVTQSYFSTAYGF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2628..2908#3014..3115#3315..4132#4720..5264#acccgctctctcctctcatctcctcctcctgtgttcgtgttcttcttcttcttgtagcagcgcttaaccaacccatcattagcgaccaaacctctctccctctccccctctgactaatcaaaattaaacaatttcgatctccaccttcctcttcttgattccagttccaccagagagagacagggtcttctctaatccatgtggtgcttgttggtctcgcttgcttcgtcttcgtcttcgtcttcctcttctcgcacctagtcccgcctcgtcgcctcctccggagaccggagccggacgggacggttgccggcgagcggcggtcgctttcttctctcggccctactcacggtcagtgattggctcccttccttcctggtgttctattgatcctttgtgggggaaaaaaggcttcgatttggggggcatgttcttgcctttttcctgttttgggggagacgagacggatttcaatatatttttttaatctggggggattatgtgtggaaaagattgttcctcgtttatctgggtagtatttggagggtcctatggggatattctttgtaaagctgggatttttaggtggtaaaataatgttgatatactttcagaaagaatatactgcagaaagtttttcagagataacagataagattaggccgtccttaatctcctttctttgcgtctgttccctccaactaggatggaaaagaatggcggattgattagtcagaaactggctgctgaatactctgagatttcatttcatttcagcctctgttgattcctcatggctgtctctaacttctttgcttgtgcttccgatttctttggcggtaaatagcctccacgaatctctggtcaacgaggtattaaaacttaaaagggcttttgtctcaggttgacccgagccatgattcgcccccctcccatgctttgcaaacaagatatgatacgcagtgctggtacaattcagagcatatgttctttcctcttttgctttgtttcagacagcaacttttagcagccacgcctctcacaagttgataaaaaaaaattttgtgttgctctcctactactcatcactttctagtgcgatatagtagtagtagtagtaggagtatatgctagcactatccacagtttagcacacagattggtggcacctgggcagatataaaagcttcacatttttatcaatgtctgcgcagttatattgtcgatttgattccttttatagatattactaggtcaagtcgtgtcgcaattactccccccctttcctccattgtagggtgttcctgtttagtccgcgggggatgctgctccctgagcctgtgtgtgtctcttgatttttctttctttgctcgcacgcagcagcagcagcatgttttgtgtcggtgttaaggaccctacataaataattcatcaaatcaagtatcttttgtctgttttggggaatctcctttgcggttcgtccaaaatttcgcgaaagaaacaattctatatgactgcttttgtcgagaggttagacatcactgctagagtagtagagcaatgctacacgtactaaatttttcttactaaacttttactaataatcatctcaaccgtttgatttaagtagatggaatttacaaaaaatctagatgcactcgtagcatgacacatcagtgttagtaagaatttagtaagaaaaagttagtacgtatagccttttccagagtagtactagcagagtgttagattgtatcacctgctcgggcagttgtaaatggtatggtgagttggtgactggtttttacagttttttcatacttgggtttatggaggatttactgttatggagacctgtaaaaaatgttacatatattgctactttctcatttggatttcggatggctgttttttttatcattatgttggtaaagttttgcatcaagaatctgaaccagcttagcaatactaatagtagatggttcagtaagctgcttccatgtccttgcaactttgcaatgctggcaattttttttcgaaaaagagcaaggctggcaatatgtgtatagcttttgtttggcaatatatgttgcgattctagcgctggtggtatctcaagatcagaaaaagtgagcatcaatgtcttactttattggttttctgactgattacattagtaaacgctgtgtccaaaagtatagagtaatactagttatattttcttaattgtcagtgactcagtatccgcagatggtttgtaaagtgcccttatttttcatggtcgttagctttagtaggtatatagtgtactcttgatggtttatcctttttttcgcccctttctcctttgtcatctgggcgcacgttgctatgcagtttcatgagatggttagtgcgtatccaactattctgtcggcaataaagttccacaatcttcaaaattttctaccattctgatgccaactcctactgcactatcagctcttggtttccagctcagttagtggttagaaattgcagcctacatgaaaaagtaactaatagattttctcgtctccaccttttatagttattttcagtgactcttcttccaaaccaagtggtaaagccaggattctaccattacaatgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcaggtactgtacccattgcttgaaatttgtcaccttattatgcttatcgtttactagtttatgataataaatgttcatagtctggcttcttctgtggctttcatacagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccggtaaggatctctgatttattattatttatgctcaagtaatgctgctttttctgaatgttcaacaaactttgatgatcaggttcaacatgagttattgtttttatacactgttttgcatgctattaacttgttgcttctttgtgtattttttactctgcatcccagaatattagaataaccttctgttttgtgtttacagtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtatattctctcttttctatctattctgaatcttaaattgggttaaatctccattatctagaagtatagcaatgaaaattctacaaaaagcaaggggcagccatccctagttctgcttctggtgctgttatctggtcagacatgataccaacgggatttatcagaaataactagataagtttgtgtgacatattatttcaaaaaagaaatatttattagtcttgccccttcaatcttaaacctctttttgacaagagctccttattatgtaaaaaacataaaaatcacacggttgcaatgtatacatcaatattattaagcacatgggggagtaattattgtgaatgtttgtggtccactccttgaactctttgctgcttactcattatgattatcttaaaactctatcagatgatatatgtttctttttgtgtatgccaaccatgccagactcaccaataacatttttattctttaatatctctggctaacatacaattattttgttttaggacacagaaagtttacttattacggtgatgtttaagaaacactaaaatctttttcttttttctattttgttctaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttgatacaaagaaacatattagatgctagcagctgcatgtaattacctttttacctcttgtttttcagctttactgtagtctccaccggaagaaaacgtttggtttagtctcttgaataatctattttcttatcctgtttgtgactatctagaatctatgtaaatggcccagctctagtgaaagttatggtgatgttatcaggaaagaaaatgtggtgtttcccgtaacaagtgctgcaaagtattattcagactc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062995.1 RefSeq:Os05g0586200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0586200&diff=169566 2014-05-18T18:11:26Z

<p>Shixing: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information<br /> ==Annotated Information==<br /> ===Function===<br /> [[File:Expression.figure1.png|thumb|right|'''Figure1:'''''Gene expression and hormone content in wild type and OsJAR1 TOS17 insertion lines after simulated herbivory (WOS)(frome reference&lt;ref name=ref1/&gt;).'']]<br /> [[File:Expression.figure2.png|thumb|right|'''Figure2:'''''The expression levels of OsJAR1 and OsJAR2 mRNA and the accumulation of JA and JA-amino acid conjugates in response to wounding(from reference&lt;ref name=ref2/&gt;).'']]<br /> OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice by the use of an osjar1 Tos17 mutant.&lt;ref name=ref1/&gt;&lt;ref name=ref4/&gt;It is involved in phytochrome and jasmonate signalling. In far-red and blue lights, osjar1 coleoptiles were longer if compared with the wild type (WT), indicating that OsJar1participates in the suppression of coleoptile elongation in these light conditions, while the mutant did not show a clear phenotype in red light.OsJar1 modulates light and JA signalling in the photomorphogenesis of rice.&lt;ref name=ref3/&gt; Light can induce jasmonate-isoleucine conjugation via OsJAR1-dependent pathways in rice. OsJAR1 is the most important JA-Ile conjugating enzyme in the wounding response during the vegetative stage.&lt;ref name=ref5/&gt;Plants produce jasmonic acid (JA) and its amino acid conjugate, jasmonoyl‐L‐isoleucine (JA‐Ile) as major defense signals in response to wounding and herbivory. OsJAR1‐deficient plants have strongly reduced JA‐Ile levels after WOS&lt;ref name=ref1/&gt;.<br /> <br /> ===Expression===<br /> <br /> (1)OsJar1 transcripts were up-regulated transiently in response to treatment with exogenous methyl-jasmonic acid (MeJA)&lt;ref name=ref3/&gt;.The expression levels of OsJAR1 and OsJAR2 were induced in response to wounding with the concomitant accumulation of JA-Ile.&lt;ref name=ref2/&gt;<br /> <br /> (2)Real-time PCR was conducted according to the QuantiTect SYBR Green PCR handbook (Qiagen). PCR was perfomed in ABI PRISM 7000 cycler (PE Applied Biosystems, Warrington, UK) using QuantiTect SYBR green-mastermix with HotStar Taq DNA polymerase following the recommended conditions [15 min 95 °C, 40× (15 s, 94 °C; 30 s, 60 °C; 30 s, 72 °C)]. JAR1F2 and JAR1R2 were used as primers to amplify OsJar1 cDNA, Rubg237F and Rubg304R for ubiquitin, which was used as the endogenous control gene.<br /> <br /> For RT-PCR, first-strand cDNA synthesis kit for RT-PCR (Roche, Mannheim, Germany) was performed according to the manufacturer's description. A 1 µL aliquot of a 1:10 dilution of the RT product was used as a template in the subsequent PCR reaction. For amplification of OsJAR1 cDNA, the primers JAR1F1 and JAR1R2 were used, for ubiquitin Rubg237F and Rubg 304R. The PCR conditions were 1 min 94 °C, 30× (94 °C, 1 min; 60 °C, 1 min; 72 °C, 2 min), 72 °C, 7 min.&lt;ref name=ref3/&gt;<br /> <br /> Primers used in real-time PCR and RT-PCR:<br /> <br /> JAR1F1: TAAGGCATTACGCAGGGAAC<br /> <br /> JAR1F2: AGTGCTGAGCATCAACATCG<br /> <br /> JAR1R1: CCAGATAACAGCACCAGAAGC<br /> <br /> JAR1R2: CACCACCAAGGCTTAGGAAA<br /> <br /> Rubg237F: GAGCCTCTGTTCGTCAAGTA<br /> <br /> Rubg304R: ACTCGATGGTCCATTAAACC&lt;ref name=ref3/&gt;<br /> <br /> <br /> ===Evolution===<br /> OsJar1 belongs to the GH3 family in rice. Another member of this family in Arabidopsis, DWARF IN LIGHT 2 (DFL2), showed 47% identities. Dfl2 was shown to be a light-dependent gene, but its gene product did not show enzymatic activity on plant hormones, presumably (Takase et al. 2003). OsJAR1 contained three domains described to be important for the enzymatic function of GH3 proteins (Chang, Xiang &amp; Dunaway-Mariano 1997; Staswick et al. 2002; Terol et al. 2006).&lt;ref name=ref3/&gt;<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0586200|<br /> Description = GH3 auxin-responsive promoter family protein|<br /> Version = NM_001062995.1 GI:115465720 GeneID:4339756|<br /> Length = 5516 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0586200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:29281780..29287295|<br /> CDS = 29284407..29284687,29284793..29284894,29285094..29285911,29286499..29287043|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccgtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MTICSCEETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNF GLGGRTDAESYKSCIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKP KFIPFNDELLETTLQIYRTSYAFRNREYPIGQGKALQFVYGSKQVITKGGILATTATT NLYRRQRYKEGMKDIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAH SLVHAFQTFEEVWEDLCTDIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIG LSNWYGVIPALWPNAKYVYGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNI DPTVPPEQVTYAVLPQVGYFEFIPLEKPIGEETENSASIHYIESDPVGLTEVEVGKIY EVVITNFAGLYRYRLGDVVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVE EASKFLEGEKLEVMDFTSFVERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDA GYTGSRKIKTIGPLELRILRKGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQIL SRNVTQSYFSTAYGF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2628..2908#3014..3115#3315..4132#4720..5264#acccgctctctcctctcatctcctcctcctgtgttcgtgttcttcttcttcttgtagcagcgcttaaccaacccatcattagcgaccaaacctctctccctctccccctctgactaatcaaaattaaacaatttcgatctccaccttcctcttcttgattccagttccaccagagagagacagggtcttctctaatccatgtggtgcttgttggtctcgcttgcttcgtcttcgtcttcgtcttcctcttctcgcacctagtcccgcctcgtcgcctcctccggagaccggagccggacgggacggttgccggcgagcggcggtcgctttcttctctcggccctactcacggtcagtgattggctcccttccttcctggtgttctattgatcctttgtgggggaaaaaaggcttcgatttggggggcatgttcttgcctttttcctgttttgggggagacgagacggatttcaatatatttttttaatctggggggattatgtgtggaaaagattgttcctcgtttatctgggtagtatttggagggtcctatggggatattctttgtaaagctgggatttttaggtggtaaaataatgttgatatactttcagaaagaatatactgcagaaagtttttcagagataacagataagattaggccgtccttaatctcctttctttgcgtctgttccctccaactaggatggaaaagaatggcggattgattagtcagaaactggctgctgaatactctgagatttcatttcatttcagcctctgttgattcctcatggctgtctctaacttctttgcttgtgcttccgatttctttggcggtaaatagcctccacgaatctctggtcaacgaggtattaaaacttaaaagggcttttgtctcaggttgacccgagccatgattcgcccccctcccatgctttgcaaacaagatatgatacgcagtgctggtacaattcagagcatatgttctttcctcttttgctttgtttcagacagcaacttttagcagccacgcctctcacaagttgataaaaaaaaattttgtgttgctctcctactactcatcactttctagtgcgatatagtagtagtagtagtaggagtatatgctagcactatccacagtttagcacacagattggtggcacctgggcagatataaaagcttcacatttttatcaatgtctgcgcagttatattgtcgatttgattccttttatagatattactaggtcaagtcgtgtcgcaattactccccccctttcctccattgtagggtgttcctgtttagtccgcgggggatgctgctccctgagcctgtgtgtgtctcttgatttttctttctttgctcgcacgcagcagcagcagcatgttttgtgtcggtgttaaggaccctacataaataattcatcaaatcaagtatcttttgtctgttttggggaatctcctttgcggttcgtccaaaatttcgcgaaagaaacaattctatatgactgcttttgtcgagaggttagacatcactgctagagtagtagagcaatgctacacgtactaaatttttcttactaaacttttactaataatcatctcaaccgtttgatttaagtagatggaatttacaaaaaatctagatgcactcgtagcatgacacatcagtgttagtaagaatttagtaagaaaaagttagtacgtatagccttttccagagtagtactagcagagtgttagattgtatcacctgctcgggcagttgtaaatggtatggtgagttggtgactggtttttacagttttttcatacttgggtttatggaggatttactgttatggagacctgtaaaaaatgttacatatattgctactttctcatttggatttcggatggctgttttttttatcattatgttggtaaagttttgcatcaagaatctgaaccagcttagcaatactaatagtagatggttcagtaagctgcttccatgtccttgcaactttgcaatgctggcaattttttttcgaaaaagagcaaggctggcaatatgtgtatagcttttgtttggcaatatatgttgcgattctagcgctggtggtatctcaagatcagaaaaagtgagcatcaatgtcttactttattggttttctgactgattacattagtaaacgctgtgtccaaaagtatagagtaatactagttatattttcttaattgtcagtgactcagtatccgcagatggtttgtaaagtgcccttatttttcatggtcgttagctttagtaggtatatagtgtactcttgatggtttatcctttttttcgcccctttctcctttgtcatctgggcgcacgttgctatgcagtttcatgagatggttagtgcgtatccaactattctgtcggcaataaagttccacaatcttcaaaattttctaccattctgatgccaactcctactgcactatcagctcttggtttccagctcagttagtggttagaaattgcagcctacatgaaaaagtaactaatagattttctcgtctccaccttttatagttattttcagtgactcttcttccaaaccaagtggtaaagccaggattctaccattacaatgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcaggtactgtacccattgcttgaaatttgtcaccttattatgcttatcgtttactagtttatgataataaatgttcatagtctggcttcttctgtggctttcatacagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccggtaaggatctctgatttattattatttatgctcaagtaatgctgctttttctgaatgttcaacaaactttgatgatcaggttcaacatgagttattgtttttatacactgttttgcatgctattaacttgttgcttctttgtgtattttttactctgcatcccagaatattagaataaccttctgttttgtgtttacagtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtatattctctcttttctatctattctgaatcttaaattgggttaaatctccattatctagaagtatagcaatgaaaattctacaaaaagcaaggggcagccatccctagttctgcttctggtgctgttatctggtcagacatgataccaacgggatttatcagaaataactagataagtttgtgtgacatattatttcaaaaaagaaatatttattagtcttgccccttcaatcttaaacctctttttgacaagagctccttattatgtaaaaaacataaaaatcacacggttgcaatgtatacatcaatattattaagcacatgggggagtaattattgtgaatgtttgtggtccactccttgaactctttgctgcttactcattatgattatcttaaaactctatcagatgatatatgtttctttttgtgtatgccaaccatgccagactcaccaataacatttttattctttaatatctctggctaacatacaattattttgttttaggacacagaaagtttacttattacggtgatgtttaagaaacactaaaatctttttcttttttctattttgttctaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttgatacaaagaaacatattagatgctagcagctgcatgtaattacctttttacctcttgtttttcagctttactgtagtctccaccggaagaaaacgtttggtttagtctcttgaataatctattttcttatcctgtttgtgactatctagaatctatgtaaatggcccagctctagtgaaagttatggtgatgttatcaggaaagaaaatgtggtgtttcccgtaacaagtgctgcaaagtattattcagactc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062995.1 RefSeq:Os05g0586200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0586200&diff=169565 2014-05-18T18:10:38Z

<p>Shixing: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information<br /> ==Annotated Information==<br /> ===Function===<br /> [[File:Expression.figure1.png|thumb|right|'''Figure1:'''''Gene expression and hormone content in wild type and OsJAR1 TOS17 insertion lines after simulated herbivory (WOS)(frome reference&lt;ref name=ref1/&gt;).'']]<br /> [[File:Expression.figure2.png|thumb|right|'''Figure2:'''''The expression levels of OsJAR1 and OsJAR2 mRNA and the accumulation of JA and JA-amino acid conjugates in response to wounding(from reference&lt;ref name=ref2/&gt;).'']]<br /> OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice by the use of an osjar1 Tos17 mutant.&lt;ref name=ref1/&gt;&lt;ref name=ref4/&gt;It is involved in phytochrome and jasmonate signalling. In far-red and blue lights, osjar1 coleoptiles were longer if compared with the wild type (WT), indicating that OsJar1participates in the suppression of coleoptile elongation in these light conditions, while the mutant did not show a clear phenotype in red light.OsJar1 modulates light and JA signalling in the photomorphogenesis of rice.&lt;ref name=ref3/&gt; Light can induce jasmonate-isoleucine conjugation via OsJAR1-dependent pathways in rice. OsJAR1 is the most important JA-Ile conjugating enzyme in the wounding response during the vegetative stage.&lt;ref name=ref5/&gt;Plants produce jasmonic acid (JA) and its amino acid conjugate, jasmonoyl‐L‐isoleucine (JA‐Ile) as major defense signals in response to wounding and herbivory. OsJAR1‐deficient plants have strongly reduced JA‐Ile levels after WOS&lt;ref name=ref1/&gt;.<br /> <br /> ===Expression===<br /> <br /> (1)OsJar1 transcripts were up-regulated transiently in response to treatment with exogenous methyl-jasmonic acid (MeJA)&lt;ref name=ref3/&gt;.The expression levels of OsJAR1 and OsJAR2 were induced in response to wounding with the concomitant accumulation of JA-Ile.&lt;ref name=ref2/&gt;<br /> <br /> (2)Real-time PCR was conducted according to the QuantiTect SYBR Green PCR handbook (Qiagen). PCR was perfomed in ABI PRISM 7000 cycler (PE Applied Biosystems, Warrington, UK) using QuantiTect SYBR green-mastermix with HotStar Taq DNA polymerase following the recommended conditions [15 min 95 °C, 40× (15 s, 94 °C; 30 s, 60 °C; 30 s, 72 °C)]. JAR1F2 and JAR1R2 were used as primers to amplify OsJar1 cDNA, Rubg237F and Rubg304R for ubiquitin, which was used as the endogenous control gene.<br /> <br /> For RT-PCR, first-strand cDNA synthesis kit for RT-PCR (Roche, Mannheim, Germany) was performed according to the manufacturer's description. A 1 µL aliquot of a 1:10 dilution of the RT product was used as a template in the subsequent PCR reaction. For amplification of OsJAR1 cDNA, the primers JAR1F1 and JAR1R2 were used, for ubiquitin Rubg237F and Rubg 304R. The PCR conditions were 1 min 94 °C, 30× (94 °C, 1 min; 60 °C, 1 min; 72 °C, 2 min), 72 °C, 7 min.&lt;ref name=ref3/&gt;<br /> <br /> Primers used in real-time PCR and RT-PCR:<br /> <br /> JAR1F1: TAAGGCATTACGCAGGGAAC<br /> <br /> JAR1F2: AGTGCTGAGCATCAACATCG<br /> <br /> JAR1R1: CCAGATAACAGCACCAGAAGC<br /> <br /> JAR1R2: CACCACCAAGGCTTAGGAAA<br /> <br /> Rubg237F: GAGCCTCTGTTCGTCAAGTA<br /> <br /> Rubg304R: ACTCGATGGTCCATTAAACC&lt;ref name=ref3/&gt;<br /> <br /> <br /> ===Evolution===<br /> OsJar1 belongs to the GH3 family in rice. Another member of this family in Arabidopsis, DWARF IN LIGHT 2 (DFL2), showed 47% identities. Dfl2 was shown to be a light-dependent gene, but its gene product did not show enzymatic activity on plant hormones, presumably (Takase et al. 2003). OsJAR1 contained three domains described to be important for the enzymatic function of GH3 proteins (Chang, Xiang &amp; Dunaway-Mariano 1997; Staswick et al. 2002; Terol et al. 2006).&lt;ref name=ref3/&gt;<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ===Expression===<br /> <br /> (1)OsJar1 transcripts were up-regulated transiently in response to treatment with exogenous methyl-jasmonic acid (MeJA)&lt;ref name=ref3/&gt;.The expression levels of OsJAR1 and OsJAR2 were induced in response to wounding with the concomitant accumulation of JA-Ile.&lt;ref name=ref2/&gt;<br /> <br /> (2)Real-time PCR was conducted according to the QuantiTect SYBR Green PCR handbook (Qiagen). PCR was perfomed in ABI PRISM 7000 cycler (PE Applied Biosystems, Warrington, UK) using QuantiTect SYBR green-mastermix with HotStar Taq DNA polymerase following the recommended conditions [15 min 95 °C, 40× (15 s, 94 °C; 30 s, 60 °C; 30 s, 72 °C)]. JAR1F2 and JAR1R2 were used as primers to amplify OsJar1 cDNA, Rubg237F and Rubg304R for ubiquitin, which was used as the endogenous control gene.<br /> <br /> For RT-PCR, first-strand cDNA synthesis kit for RT-PCR (Roche, Mannheim, Germany) was performed according to the manufacturer's description. A 1 µL aliquot of a 1:10 dilution of the RT product was used as a template in the subsequent PCR reaction. For amplification of OsJAR1 cDNA, the primers JAR1F1 and JAR1R2 were used, for ubiquitin Rubg237F and Rubg 304R. The PCR conditions were 1 min 94 °C, 30× (94 °C, 1 min; 60 °C, 1 min; 72 °C, 2 min), 72 °C, 7 min.&lt;ref name=ref3/&gt;<br /> <br /> Primers used in real-time PCR and RT-PCR:<br /> <br /> JAR1F1: TAAGGCATTACGCAGGGAAC<br /> <br /> JAR1F2: AGTGCTGAGCATCAACATCG<br /> <br /> JAR1R1: CCAGATAACAGCACCAGAAGC<br /> <br /> JAR1R2: CACCACCAAGGCTTAGGAAA<br /> <br /> Rubg237F: GAGCCTCTGTTCGTCAAGTA<br /> <br /> Rubg304R: ACTCGATGGTCCATTAAACC&lt;ref name=ref3/&gt;<br /> <br /> <br /> ===Evolution===<br /> OsJar1 belongs to the GH3 family in rice. Another member of this family in Arabidopsis, DWARF IN LIGHT 2 (DFL2), showed 47% identities. Dfl2 was shown to be a light-dependent gene, but its gene product did not show enzymatic activity on plant hormones, presumably (Takase et al. 2003). OsJAR1 contained three domains described to be important for the enzymatic function of GH3 proteins (Chang, Xiang &amp; Dunaway-Mariano 1997; Staswick et al. 2002; Terol et al. 2006).&lt;ref name=ref3/&gt;<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0586200|<br /> Description = GH3 auxin-responsive promoter family protein|<br /> Version = NM_001062995.1 GI:115465720 GeneID:4339756|<br /> Length = 5516 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0586200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:29281780..29287295|<br /> CDS = 29284407..29284687,29284793..29284894,29285094..29285911,29286499..29287043|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccgtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MTICSCEETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNF GLGGRTDAESYKSCIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKP KFIPFNDELLETTLQIYRTSYAFRNREYPIGQGKALQFVYGSKQVITKGGILATTATT NLYRRQRYKEGMKDIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAH SLVHAFQTFEEVWEDLCTDIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIG LSNWYGVIPALWPNAKYVYGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNI DPTVPPEQVTYAVLPQVGYFEFIPLEKPIGEETENSASIHYIESDPVGLTEVEVGKIY EVVITNFAGLYRYRLGDVVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVE EASKFLEGEKLEVMDFTSFVERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDA GYTGSRKIKTIGPLELRILRKGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQIL SRNVTQSYFSTAYGF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2628..2908#3014..3115#3315..4132#4720..5264#acccgctctctcctctcatctcctcctcctgtgttcgtgttcttcttcttcttgtagcagcgcttaaccaacccatcattagcgaccaaacctctctccctctccccctctgactaatcaaaattaaacaatttcgatctccaccttcctcttcttgattccagttccaccagagagagacagggtcttctctaatccatgtggtgcttgttggtctcgcttgcttcgtcttcgtcttcgtcttcctcttctcgcacctagtcccgcctcgtcgcctcctccggagaccggagccggacgggacggttgccggcgagcggcggtcgctttcttctctcggccctactcacggtcagtgattggctcccttccttcctggtgttctattgatcctttgtgggggaaaaaaggcttcgatttggggggcatgttcttgcctttttcctgttttgggggagacgagacggatttcaatatatttttttaatctggggggattatgtgtggaaaagattgttcctcgtttatctgggtagtatttggagggtcctatggggatattctttgtaaagctgggatttttaggtggtaaaataatgttgatatactttcagaaagaatatactgcagaaagtttttcagagataacagataagattaggccgtccttaatctcctttctttgcgtctgttccctccaactaggatggaaaagaatggcggattgattagtcagaaactggctgctgaatactctgagatttcatttcatttcagcctctgttgattcctcatggctgtctctaacttctttgcttgtgcttccgatttctttggcggtaaatagcctccacgaatctctggtcaacgaggtattaaaacttaaaagggcttttgtctcaggttgacccgagccatgattcgcccccctcccatgctttgcaaacaagatatgatacgcagtgctggtacaattcagagcatatgttctttcctcttttgctttgtttcagacagcaacttttagcagccacgcctctcacaagttgataaaaaaaaattttgtgttgctctcctactactcatcactttctagtgcgatatagtagtagtagtagtaggagtatatgctagcactatccacagtttagcacacagattggtggcacctgggcagatataaaagcttcacatttttatcaatgtctgcgcagttatattgtcgatttgattccttttatagatattactaggtcaagtcgtgtcgcaattactccccccctttcctccattgtagggtgttcctgtttagtccgcgggggatgctgctccctgagcctgtgtgtgtctcttgatttttctttctttgctcgcacgcagcagcagcagcatgttttgtgtcggtgttaaggaccctacataaataattcatcaaatcaagtatcttttgtctgttttggggaatctcctttgcggttcgtccaaaatttcgcgaaagaaacaattctatatgactgcttttgtcgagaggttagacatcactgctagagtagtagagcaatgctacacgtactaaatttttcttactaaacttttactaataatcatctcaaccgtttgatttaagtagatggaatttacaaaaaatctagatgcactcgtagcatgacacatcagtgttagtaagaatttagtaagaaaaagttagtacgtatagccttttccagagtagtactagcagagtgttagattgtatcacctgctcgggcagttgtaaatggtatggtgagttggtgactggtttttacagttttttcatacttgggtttatggaggatttactgttatggagacctgtaaaaaatgttacatatattgctactttctcatttggatttcggatggctgttttttttatcattatgttggtaaagttttgcatcaagaatctgaaccagcttagcaatactaatagtagatggttcagtaagctgcttccatgtccttgcaactttgcaatgctggcaattttttttcgaaaaagagcaaggctggcaatatgtgtatagcttttgtttggcaatatatgttgcgattctagcgctggtggtatctcaagatcagaaaaagtgagcatcaatgtcttactttattggttttctgactgattacattagtaaacgctgtgtccaaaagtatagagtaatactagttatattttcttaattgtcagtgactcagtatccgcagatggtttgtaaagtgcccttatttttcatggtcgttagctttagtaggtatatagtgtactcttgatggtttatcctttttttcgcccctttctcctttgtcatctgggcgcacgttgctatgcagtttcatgagatggttagtgcgtatccaactattctgtcggcaataaagttccacaatcttcaaaattttctaccattctgatgccaactcctactgcactatcagctcttggtttccagctcagttagtggttagaaattgcagcctacatgaaaaagtaactaatagattttctcgtctccaccttttatagttattttcagtgactcttcttccaaaccaagtggtaaagccaggattctaccattacaatgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcaggtactgtacccattgcttgaaatttgtcaccttattatgcttatcgtttactagtttatgataataaatgttcatagtctggcttcttctgtggctttcatacagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccggtaaggatctctgatttattattatttatgctcaagtaatgctgctttttctgaatgttcaacaaactttgatgatcaggttcaacatgagttattgtttttatacactgttttgcatgctattaacttgttgcttctttgtgtattttttactctgcatcccagaatattagaataaccttctgttttgtgtttacagtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtatattctctcttttctatctattctgaatcttaaattgggttaaatctccattatctagaagtatagcaatgaaaattctacaaaaagcaaggggcagccatccctagttctgcttctggtgctgttatctggtcagacatgataccaacgggatttatcagaaataactagataagtttgtgtgacatattatttcaaaaaagaaatatttattagtcttgccccttcaatcttaaacctctttttgacaagagctccttattatgtaaaaaacataaaaatcacacggttgcaatgtatacatcaatattattaagcacatgggggagtaattattgtgaatgtttgtggtccactccttgaactctttgctgcttactcattatgattatcttaaaactctatcagatgatatatgtttctttttgtgtatgccaaccatgccagactcaccaataacatttttattctttaatatctctggctaacatacaattattttgttttaggacacagaaagtttacttattacggtgatgtttaagaaacactaaaatctttttcttttttctattttgttctaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttgatacaaagaaacatattagatgctagcagctgcatgtaattacctttttacctcttgtttttcagctttactgtagtctccaccggaagaaaacgtttggtttagtctcttgaataatctattttcttatcctgtttgtgactatctagaatctatgtaaatggcccagctctagtgaaagttatggtgatgttatcaggaaagaaaatgtggtgtttcccgtaacaagtgctgcaaagtattattcagactc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062995.1 RefSeq:Os05g0586200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0586200&diff=169564 2014-05-18T18:09:38Z

<p>Shixing: /* Annotated InformationFile:http://ricewiki.big.ac.cn/skins/common/images/button_media.png */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information<br /> ===Function===<br /> [[File:Expression.figure1.png|thumb|right|'''Figure1:'''''Gene expression and hormone content in wild type and OsJAR1 TOS17 insertion lines after simulated herbivory (WOS)(frome reference&lt;ref name=ref1/&gt;).'']]<br /> [[File:Expression.figure2.png|thumb|right|'''Figure2:'''''The expression levels of OsJAR1 and OsJAR2 mRNA and the accumulation of JA and JA-amino acid conjugates in response to wounding(from reference&lt;ref name=ref2/&gt;).'']]<br /> OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice by the use of an osjar1 Tos17 mutant.&lt;ref name=ref1/&gt;&lt;ref name=ref4/&gt;It is involved in phytochrome and jasmonate signalling. In far-red and blue lights, osjar1 coleoptiles were longer if compared with the wild type (WT), indicating that OsJar1participates in the suppression of coleoptile elongation in these light conditions, while the mutant did not show a clear phenotype in red light.OsJar1 modulates light and JA signalling in the photomorphogenesis of rice.&lt;ref name=ref3/&gt; Light can induce jasmonate-isoleucine conjugation via OsJAR1-dependent pathways in rice. OsJAR1 is the most important JA-Ile conjugating enzyme in the wounding response during the vegetative stage.&lt;ref name=ref5/&gt;Plants produce jasmonic acid (JA) and its amino acid conjugate, jasmonoyl‐L‐isoleucine (JA‐Ile) as major defense signals in response to wounding and herbivory. OsJAR1‐deficient plants have strongly reduced JA‐Ile levels after WOS&lt;ref name=ref1/&gt;.<br /> <br /> ===Expression===<br /> <br /> (1)OsJar1 transcripts were up-regulated transiently in response to treatment with exogenous methyl-jasmonic acid (MeJA)&lt;ref name=ref3/&gt;.The expression levels of OsJAR1 and OsJAR2 were induced in response to wounding with the concomitant accumulation of JA-Ile.&lt;ref name=ref2/&gt;<br /> <br /> (2)Real-time PCR was conducted according to the QuantiTect SYBR Green PCR handbook (Qiagen). PCR was perfomed in ABI PRISM 7000 cycler (PE Applied Biosystems, Warrington, UK) using QuantiTect SYBR green-mastermix with HotStar Taq DNA polymerase following the recommended conditions [15 min 95 °C, 40× (15 s, 94 °C; 30 s, 60 °C; 30 s, 72 °C)]. JAR1F2 and JAR1R2 were used as primers to amplify OsJar1 cDNA, Rubg237F and Rubg304R for ubiquitin, which was used as the endogenous control gene.<br /> <br /> For RT-PCR, first-strand cDNA synthesis kit for RT-PCR (Roche, Mannheim, Germany) was performed according to the manufacturer's description. A 1 µL aliquot of a 1:10 dilution of the RT product was used as a template in the subsequent PCR reaction. For amplification of OsJAR1 cDNA, the primers JAR1F1 and JAR1R2 were used, for ubiquitin Rubg237F and Rubg 304R. The PCR conditions were 1 min 94 °C, 30× (94 °C, 1 min; 60 °C, 1 min; 72 °C, 2 min), 72 °C, 7 min.&lt;ref name=ref3/&gt;<br /> <br /> Primers used in real-time PCR and RT-PCR:<br /> <br /> JAR1F1: TAAGGCATTACGCAGGGAAC<br /> <br /> JAR1F2: AGTGCTGAGCATCAACATCG<br /> <br /> JAR1R1: CCAGATAACAGCACCAGAAGC<br /> <br /> JAR1R2: CACCACCAAGGCTTAGGAAA<br /> <br /> Rubg237F: GAGCCTCTGTTCGTCAAGTA<br /> <br /> Rubg304R: ACTCGATGGTCCATTAAACC&lt;ref name=ref3/&gt;<br /> <br /> <br /> ===Evolution===<br /> OsJar1 belongs to the GH3 family in rice. Another member of this family in Arabidopsis, DWARF IN LIGHT 2 (DFL2), showed 47% identities. Dfl2 was shown to be a light-dependent gene, but its gene product did not show enzymatic activity on plant hormones, presumably (Takase et al. 2003). OsJAR1 contained three domains described to be important for the enzymatic function of GH3 proteins (Chang, Xiang &amp; Dunaway-Mariano 1997; Staswick et al. 2002; Terol et al. 2006).&lt;ref name=ref3/&gt;<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0586200|<br /> Description = GH3 auxin-responsive promoter family protein|<br /> Version = NM_001062995.1 GI:115465720 GeneID:4339756|<br /> Length = 5516 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0586200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:29281780..29287295|<br /> CDS = 29284407..29284687,29284793..29284894,29285094..29285911,29286499..29287043|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccgtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MTICSCEETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNF GLGGRTDAESYKSCIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKP KFIPFNDELLETTLQIYRTSYAFRNREYPIGQGKALQFVYGSKQVITKGGILATTATT NLYRRQRYKEGMKDIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAH SLVHAFQTFEEVWEDLCTDIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIG LSNWYGVIPALWPNAKYVYGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNI DPTVPPEQVTYAVLPQVGYFEFIPLEKPIGEETENSASIHYIESDPVGLTEVEVGKIY EVVITNFAGLYRYRLGDVVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVE EASKFLEGEKLEVMDFTSFVERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDA GYTGSRKIKTIGPLELRILRKGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQIL SRNVTQSYFSTAYGF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2628..2908#3014..3115#3315..4132#4720..5264#acccgctctctcctctcatctcctcctcctgtgttcgtgttcttcttcttcttgtagcagcgcttaaccaacccatcattagcgaccaaacctctctccctctccccctctgactaatcaaaattaaacaatttcgatctccaccttcctcttcttgattccagttccaccagagagagacagggtcttctctaatccatgtggtgcttgttggtctcgcttgcttcgtcttcgtcttcgtcttcctcttctcgcacctagtcccgcctcgtcgcctcctccggagaccggagccggacgggacggttgccggcgagcggcggtcgctttcttctctcggccctactcacggtcagtgattggctcccttccttcctggtgttctattgatcctttgtgggggaaaaaaggcttcgatttggggggcatgttcttgcctttttcctgttttgggggagacgagacggatttcaatatatttttttaatctggggggattatgtgtggaaaagattgttcctcgtttatctgggtagtatttggagggtcctatggggatattctttgtaaagctgggatttttaggtggtaaaataatgttgatatactttcagaaagaatatactgcagaaagtttttcagagataacagataagattaggccgtccttaatctcctttctttgcgtctgttccctccaactaggatggaaaagaatggcggattgattagtcagaaactggctgctgaatactctgagatttcatttcatttcagcctctgttgattcctcatggctgtctctaacttctttgcttgtgcttccgatttctttggcggtaaatagcctccacgaatctctggtcaacgaggtattaaaacttaaaagggcttttgtctcaggttgacccgagccatgattcgcccccctcccatgctttgcaaacaagatatgatacgcagtgctggtacaattcagagcatatgttctttcctcttttgctttgtttcagacagcaacttttagcagccacgcctctcacaagttgataaaaaaaaattttgtgttgctctcctactactcatcactttctagtgcgatatagtagtagtagtagtaggagtatatgctagcactatccacagtttagcacacagattggtggcacctgggcagatataaaagcttcacatttttatcaatgtctgcgcagttatattgtcgatttgattccttttatagatattactaggtcaagtcgtgtcgcaattactccccccctttcctccattgtagggtgttcctgtttagtccgcgggggatgctgctccctgagcctgtgtgtgtctcttgatttttctttctttgctcgcacgcagcagcagcagcatgttttgtgtcggtgttaaggaccctacataaataattcatcaaatcaagtatcttttgtctgttttggggaatctcctttgcggttcgtccaaaatttcgcgaaagaaacaattctatatgactgcttttgtcgagaggttagacatcactgctagagtagtagagcaatgctacacgtactaaatttttcttactaaacttttactaataatcatctcaaccgtttgatttaagtagatggaatttacaaaaaatctagatgcactcgtagcatgacacatcagtgttagtaagaatttagtaagaaaaagttagtacgtatagccttttccagagtagtactagcagagtgttagattgtatcacctgctcgggcagttgtaaatggtatggtgagttggtgactggtttttacagttttttcatacttgggtttatggaggatttactgttatggagacctgtaaaaaatgttacatatattgctactttctcatttggatttcggatggctgttttttttatcattatgttggtaaagttttgcatcaagaatctgaaccagcttagcaatactaatagtagatggttcagtaagctgcttccatgtccttgcaactttgcaatgctggcaattttttttcgaaaaagagcaaggctggcaatatgtgtatagcttttgtttggcaatatatgttgcgattctagcgctggtggtatctcaagatcagaaaaagtgagcatcaatgtcttactttattggttttctgactgattacattagtaaacgctgtgtccaaaagtatagagtaatactagttatattttcttaattgtcagtgactcagtatccgcagatggtttgtaaagtgcccttatttttcatggtcgttagctttagtaggtatatagtgtactcttgatggtttatcctttttttcgcccctttctcctttgtcatctgggcgcacgttgctatgcagtttcatgagatggttagtgcgtatccaactattctgtcggcaataaagttccacaatcttcaaaattttctaccattctgatgccaactcctactgcactatcagctcttggtttccagctcagttagtggttagaaattgcagcctacatgaaaaagtaactaatagattttctcgtctccaccttttatagttattttcagtgactcttcttccaaaccaagtggtaaagccaggattctaccattacaatgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcaggtactgtacccattgcttgaaatttgtcaccttattatgcttatcgtttactagtttatgataataaatgttcatagtctggcttcttctgtggctttcatacagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccggtaaggatctctgatttattattatttatgctcaagtaatgctgctttttctgaatgttcaacaaactttgatgatcaggttcaacatgagttattgtttttatacactgttttgcatgctattaacttgttgcttctttgtgtattttttactctgcatcccagaatattagaataaccttctgttttgtgtttacagtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtatattctctcttttctatctattctgaatcttaaattgggttaaatctccattatctagaagtatagcaatgaaaattctacaaaaagcaaggggcagccatccctagttctgcttctggtgctgttatctggtcagacatgataccaacgggatttatcagaaataactagataagtttgtgtgacatattatttcaaaaaagaaatatttattagtcttgccccttcaatcttaaacctctttttgacaagagctccttattatgtaaaaaacataaaaatcacacggttgcaatgtatacatcaatattattaagcacatgggggagtaattattgtgaatgtttgtggtccactccttgaactctttgctgcttactcattatgattatcttaaaactctatcagatgatatatgtttctttttgtgtatgccaaccatgccagactcaccaataacatttttattctttaatatctctggctaacatacaattattttgttttaggacacagaaagtttacttattacggtgatgtttaagaaacactaaaatctttttcttttttctattttgttctaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttgatacaaagaaacatattagatgctagcagctgcatgtaattacctttttacctcttgtttttcagctttactgtagtctccaccggaagaaaacgtttggtttagtctcttgaataatctattttcttatcctgtttgtgactatctagaatctatgtaaatggcccagctctagtgaaagttatggtgatgttatcaggaaagaaaatgtggtgtttcccgtaacaagtgctgcaaagtattattcagactc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062995.1 RefSeq:Os05g0586200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os05g0586200&diff=169563 2014-05-18T18:08:41Z

<p>Shixing: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information[[File:http://ricewiki.big.ac.cn/skins/common/images/button_media.png]]==<br /> ===Function===<br /> [[File:Expression.figure1.png|thumb|right|'''Figure1:'''''Gene expression and hormone content in wild type and OsJAR1 TOS17 insertion lines after simulated herbivory (WOS)(frome reference&lt;ref name=ref1/&gt;).'']]<br /> [[File:Expression.figure2.png|thumb|right|'''Figure2:'''''The expression levels of OsJAR1 and OsJAR2 mRNA and the accumulation of JA and JA-amino acid conjugates in response to wounding(from reference&lt;ref name=ref2/&gt;).'']]<br /> OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice by the use of an osjar1 Tos17 mutant.&lt;ref name=ref1/&gt;&lt;ref name=ref4/&gt;It is involved in phytochrome and jasmonate signalling. In far-red and blue lights, osjar1 coleoptiles were longer if compared with the wild type (WT), indicating that OsJar1participates in the suppression of coleoptile elongation in these light conditions, while the mutant did not show a clear phenotype in red light.OsJar1 modulates light and JA signalling in the photomorphogenesis of rice.&lt;ref name=ref3/&gt; Light can induce jasmonate-isoleucine conjugation via OsJAR1-dependent pathways in rice. OsJAR1 is the most important JA-Ile conjugating enzyme in the wounding response during the vegetative stage.&lt;ref name=ref5/&gt;Plants produce jasmonic acid (JA) and its amino acid conjugate, jasmonoyl‐L‐isoleucine (JA‐Ile) as major defense signals in response to wounding and herbivory. OsJAR1‐deficient plants have strongly reduced JA‐Ile levels after WOS&lt;ref name=ref1/&gt;.<br /> <br /> ===Expression===<br /> <br /> (1)OsJar1 transcripts were up-regulated transiently in response to treatment with exogenous methyl-jasmonic acid (MeJA)&lt;ref name=ref3/&gt;.The expression levels of OsJAR1 and OsJAR2 were induced in response to wounding with the concomitant accumulation of JA-Ile.&lt;ref name=ref2/&gt;<br /> <br /> (2)Real-time PCR was conducted according to the QuantiTect SYBR Green PCR handbook (Qiagen). PCR was perfomed in ABI PRISM 7000 cycler (PE Applied Biosystems, Warrington, UK) using QuantiTect SYBR green-mastermix with HotStar Taq DNA polymerase following the recommended conditions [15 min 95 °C, 40× (15 s, 94 °C; 30 s, 60 °C; 30 s, 72 °C)]. JAR1F2 and JAR1R2 were used as primers to amplify OsJar1 cDNA, Rubg237F and Rubg304R for ubiquitin, which was used as the endogenous control gene.<br /> <br /> For RT-PCR, first-strand cDNA synthesis kit for RT-PCR (Roche, Mannheim, Germany) was performed according to the manufacturer's description. A 1 µL aliquot of a 1:10 dilution of the RT product was used as a template in the subsequent PCR reaction. For amplification of OsJAR1 cDNA, the primers JAR1F1 and JAR1R2 were used, for ubiquitin Rubg237F and Rubg 304R. The PCR conditions were 1 min 94 °C, 30× (94 °C, 1 min; 60 °C, 1 min; 72 °C, 2 min), 72 °C, 7 min.&lt;ref name=ref3/&gt;<br /> <br /> Primers used in real-time PCR and RT-PCR:<br /> <br /> JAR1F1: TAAGGCATTACGCAGGGAAC<br /> <br /> JAR1F2: AGTGCTGAGCATCAACATCG<br /> <br /> JAR1R1: CCAGATAACAGCACCAGAAGC<br /> <br /> JAR1R2: CACCACCAAGGCTTAGGAAA<br /> <br /> Rubg237F: GAGCCTCTGTTCGTCAAGTA<br /> <br /> Rubg304R: ACTCGATGGTCCATTAAACC&lt;ref name=ref3/&gt;<br /> <br /> <br /> ===Evolution===<br /> OsJar1 belongs to the GH3 family in rice. Another member of this family in Arabidopsis, DWARF IN LIGHT 2 (DFL2), showed 47% identities. Dfl2 was shown to be a light-dependent gene, but its gene product did not show enzymatic activity on plant hormones, presumably (Takase et al. 2003). OsJAR1 contained three domains described to be important for the enzymatic function of GH3 proteins (Chang, Xiang &amp; Dunaway-Mariano 1997; Staswick et al. 2002; Terol et al. 2006).&lt;ref name=ref3/&gt;<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os05g0586200|<br /> Description = GH3 auxin-responsive promoter family protein|<br /> Version = NM_001062995.1 GI:115465720 GeneID:4339756|<br /> Length = 5516 bp|<br /> Definition = Oryza sativa Japonica Group Os05g0586200, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|<br /> AP = Chromosome 5:29281780..29287295|<br /> CDS = 29284407..29284687,29284793..29284894,29285094..29285911,29286499..29287043|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008398:29281780..29287295<br /> source=RiceChromosome05<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccgtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MTICSCEETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNF GLGGRTDAESYKSCIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKP KFIPFNDELLETTLQIYRTSYAFRNREYPIGQGKALQFVYGSKQVITKGGILATTATT NLYRRQRYKEGMKDIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAH SLVHAFQTFEEVWEDLCTDIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIG LSNWYGVIPALWPNAKYVYGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNI DPTVPPEQVTYAVLPQVGYFEFIPLEKPIGEETENSASIHYIESDPVGLTEVEVGKIY EVVITNFAGLYRYRLGDVVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVE EASKFLEGEKLEVMDFTSFVERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDA GYTGSRKIKTIGPLELRILRKGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQIL SRNVTQSYFSTAYGF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;2628..2908#3014..3115#3315..4132#4720..5264#acccgctctctcctctcatctcctcctcctgtgttcgtgttcttcttcttcttgtagcagcgcttaaccaacccatcattagcgaccaaacctctctccctctccccctctgactaatcaaaattaaacaatttcgatctccaccttcctcttcttgattccagttccaccagagagagacagggtcttctctaatccatgtggtgcttgttggtctcgcttgcttcgtcttcgtcttcgtcttcctcttctcgcacctagtcccgcctcgtcgcctcctccggagaccggagccggacgggacggttgccggcgagcggcggtcgctttcttctctcggccctactcacggtcagtgattggctcccttccttcctggtgttctattgatcctttgtgggggaaaaaaggcttcgatttggggggcatgttcttgcctttttcctgttttgggggagacgagacggatttcaatatatttttttaatctggggggattatgtgtggaaaagattgttcctcgtttatctgggtagtatttggagggtcctatggggatattctttgtaaagctgggatttttaggtggtaaaataatgttgatatactttcagaaagaatatactgcagaaagtttttcagagataacagataagattaggccgtccttaatctcctttctttgcgtctgttccctccaactaggatggaaaagaatggcggattgattagtcagaaactggctgctgaatactctgagatttcatttcatttcagcctctgttgattcctcatggctgtctctaacttctttgcttgtgcttccgatttctttggcggtaaatagcctccacgaatctctggtcaacgaggtattaaaacttaaaagggcttttgtctcaggttgacccgagccatgattcgcccccctcccatgctttgcaaacaagatatgatacgcagtgctggtacaattcagagcatatgttctttcctcttttgctttgtttcagacagcaacttttagcagccacgcctctcacaagttgataaaaaaaaattttgtgttgctctcctactactcatcactttctagtgcgatatagtagtagtagtagtaggagtatatgctagcactatccacagtttagcacacagattggtggcacctgggcagatataaaagcttcacatttttatcaatgtctgcgcagttatattgtcgatttgattccttttatagatattactaggtcaagtcgtgtcgcaattactccccccctttcctccattgtagggtgttcctgtttagtccgcgggggatgctgctccctgagcctgtgtgtgtctcttgatttttctttctttgctcgcacgcagcagcagcagcatgttttgtgtcggtgttaaggaccctacataaataattcatcaaatcaagtatcttttgtctgttttggggaatctcctttgcggttcgtccaaaatttcgcgaaagaaacaattctatatgactgcttttgtcgagaggttagacatcactgctagagtagtagagcaatgctacacgtactaaatttttcttactaaacttttactaataatcatctcaaccgtttgatttaagtagatggaatttacaaaaaatctagatgcactcgtagcatgacacatcagtgttagtaagaatttagtaagaaaaagttagtacgtatagccttttccagagtagtactagcagagtgttagattgtatcacctgctcgggcagttgtaaatggtatggtgagttggtgactggtttttacagttttttcatacttgggtttatggaggatttactgttatggagacctgtaaaaaatgttacatatattgctactttctcatttggatttcggatggctgttttttttatcattatgttggtaaagttttgcatcaagaatctgaaccagcttagcaatactaatagtagatggttcagtaagctgcttccatgtccttgcaactttgcaatgctggcaattttttttcgaaaaagagcaaggctggcaatatgtgtatagcttttgtttggcaatatatgttgcgattctagcgctggtggtatctcaagatcagaaaaagtgagcatcaatgtcttactttattggttttctgactgattacattagtaaacgctgtgtccaaaagtatagagtaatactagttatattttcttaattgtcagtgactcagtatccgcagatggtttgtaaagtgcccttatttttcatggtcgttagctttagtaggtatatagtgtactcttgatggtttatcctttttttcgcccctttctcctttgtcatctgggcgcacgttgctatgcagtttcatgagatggttagtgcgtatccaactattctgtcggcaataaagttccacaatcttcaaaattttctaccattctgatgccaactcctactgcactatcagctcttggtttccagctcagttagtggttagaaattgcagcctacatgaaaaagtaactaatagattttctcgtctccaccttttatagttattttcagtgactcttcttccaaaccaagtggtaaagccaggattctaccattacaatgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcaggtactgtacccattgcttgaaatttgtcaccttattatgcttatcgtttactagtttatgataataaatgttcatagtctggcttcttctgtggctttcatacagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccggtaaggatctctgatttattattatttatgctcaagtaatgctgctttttctgaatgttcaacaaactttgatgatcaggttcaacatgagttattgtttttatacactgttttgcatgctattaacttgttgcttctttgtgtattttttactctgcatcccagaatattagaataaccttctgttttgtgtttacagtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtatattctctcttttctatctattctgaatcttaaattgggttaaatctccattatctagaagtatagcaatgaaaattctacaaaaagcaaggggcagccatccctagttctgcttctggtgctgttatctggtcagacatgataccaacgggatttatcagaaataactagataagtttgtgtgacatattatttcaaaaaagaaatatttattagtcttgccccttcaatcttaaacctctttttgacaagagctccttattatgtaaaaaacataaaaatcacacggttgcaatgtatacatcaatattattaagcacatgggggagtaattattgtgaatgtttgtggtccactccttgaactctttgctgcttactcattatgattatcttaaaactctatcagatgatatatgtttctttttgtgtatgccaaccatgccagactcaccaataacatttttattctttaatatctctggctaacatacaattattttgttttaggacacagaaagtttacttattacggtgatgtttaagaaacactaaaatctttttcttttttctattttgttctaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttgatacaaagaaacatattagatgctagcagctgcatgtaattacctttttacctcttgtttttcagctttactgtagtctccaccggaagaaaacgtttggtttagtctcttgaataatctattttcttatcctgtttgtgactatctagaatctatgtaaatggcccagctctagtgaaagttatggtgatgttatcaggaaagaaaatgtggtgtttcccgtaacaagtgctgcaaagtattattcagactc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062995.1 RefSeq:Os05g0586200]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 5]]<br /> [[Category:Chromosome 5]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Expression.figure2.png&diff=169561 2014-05-18T17:57:36Z

<p>Shixing: </p> <hr /> <div></div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Expresion.figure3.png&diff=169559 2014-05-18T17:43:30Z

<p>Shixing: </p> <hr /> <div></div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169555 2014-05-18T17:29:54Z

<p>Shixing: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 is a basic helix-loop-helix protein, which interacts with OsJAZ proteins in a jasmonate signalling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signalling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signalling module in the upstream signalling pathway to improve drought tolerance in rice&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> <br /> As figure1 shows, OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding&lt;ref name=&quot;ref1&quot; /&gt;。[[File:Expression.gif|thumb|right|''Figure1: OsDREB148 was rapidly induced by 15 min of exposure to MeJA and was gradually responsive to drought conditions（from reference &lt;ref name=&quot;ref1&quot; /&gt;）.'']].<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> <br /> Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> <br /> GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> <br /> School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Ju-Seok Seo1, Joungsu Joo2, Min-Jeong Kim1, Yang Do Choi1 et al.(2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.The Plant Journal<br /> Volume 65, Issue 6:907–921.&lt;/ref&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169553 2014-05-18T17:26:33Z

<p>Shixing: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 is a basic helix-loop-helix protein, which interacts with OsJAZ proteins in a jasmonate signalling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signalling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signalling module in the upstream signalling pathway to improve drought tolerance in rice&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> <br /> As figure1 shows, OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding&lt;ref name=&quot;ref1&quot; /&gt;。[[File:Expression.gif|thumb|right|''Figure1: OsDREB148 was rapidly induced by 15 min of exposure to MeJA and was gradually responsive to drought conditions（from reference &lt;ref name=&quot;ref1&quot; /&gt;）.'']].<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> <br /> Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> <br /> GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> <br /> School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> 1. Ju-Seok Seo1, Joungsu Joo2, Min-Jeong Kim1, Yang Do Choi1 et al.(2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.The Plant Journal<br /> Volume 65, Issue 6:907–921<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169552 2014-05-18T17:24:37Z

<p>Shixing: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 is a basic helix-loop-helix protein, which interacts with OsJAZ proteins in a jasmonate signalling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signalling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signalling module in the upstream signalling pathway to improve drought tolerance in rice.&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> <br /> As figure1 shows, OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding.&lt;ref name=&quot;ref1&quot; /&gt;[[File:Expression.gif|thumb|right|Figure1: OsDREB148 was rapidly induced by 15 min of exposure to MeJA and was gradually responsive to drought conditions（from reference &lt;ref name=&quot;ref1&quot; /&gt;）.]].<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> <br /> Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> <br /> GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> <br /> School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> 1. Ju-Seok Seo1, Joungsu Joo2, Min-Jeong Kim1, Yang Do Choi1 et al.(2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.The Plant Journal<br /> Volume 65, Issue 6:907–921<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169551 2014-05-18T17:23:57Z

<p>Shixing: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 is a basic helix-loop-helix protein, which interacts with OsJAZ proteins in a jasmonate signalling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signalling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signalling module in the upstream signalling pathway to improve drought tolerance in rice.&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> <br /> As figure1 shows, OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding.&lt;ref name=&quot;ref1&quot; /&gt;[[File:Expression.gif|thumb|right|Figure1: OsDREB148 was rapidly induced by 15 min of exposure to MeJA and was gradually responsive to drought conditions（from reference&lt;ref name=&quot;ref1&quot; /&gt;）.]].<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> <br /> Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> <br /> GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> <br /> School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> 1. Ju-Seok Seo1, Joungsu Joo2, Min-Jeong Kim1, Yang Do Choi1 et al.(2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.The Plant Journal<br /> Volume 65, Issue 6:907–921<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169550 2014-05-18T17:23:08Z

<p>Shixing: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 is a basic helix-loop-helix protein, which interacts with OsJAZ proteins in a jasmonate signalling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signalling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signalling module in the upstream signalling pathway to improve drought tolerance in rice.&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Expression===<br /> <br /> As figure1 shows, OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding.[[File:Expression.gif|thumb|right|Figure1: OsDREB148 was rapidly induced by 15 min of exposure to MeJA and was gradually responsive to drought conditions（from reference&lt;ref name=&quot;ref1&quot; /&gt;）.]]&lt;ref name=&quot;ref1&quot; /&gt;.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> <br /> Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> <br /> GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> <br /> School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> 1. Ju-Seok Seo1, Joungsu Joo2, Min-Jeong Kim1, Yang Do Choi1 et al.(2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.The Plant Journal<br /> Volume 65, Issue 6:907–921<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Expression.figure1.png&diff=169547 2014-05-18T17:15:40Z

<p>Shixing: </p> <hr /> <div></div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169542 2014-05-18T16:20:42Z

<p>Shixing: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 is a basic helix-loop-helix protein, which interacts with OsJAZ proteins in a jasmonate signalling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signalling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signalling module in the upstream signalling pathway to improve drought tolerance in rice.[1]<br /> <br /> ===Expression===<br /> <br /> As figure1 shows, OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding.[[File:Expression.gif|thumb|right|Figure1: OsDREB148 was rapidly induced by 15 min of exposure to MeJA and was gradually responsive to drought conditions]][1]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> <br /> Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> <br /> GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> <br /> School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> 1. Ju-Seok Seo1, Joungsu Joo2, Min-Jeong Kim1, Yang Do Choi1 et al.(2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.The Plant Journal<br /> Volume 65, Issue 6:907–921<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169532 2014-05-18T15:28:38Z

<p>Shixing: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 is a basic helix-loop-helix protein, which interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signaling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signaling module in the upstream signaling pathway to improve drought tolerance in rice.[1]<br /> <br /> ===Expression===<br /> <br /> As figure1 shows, OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding.[[File:Expression.gif|thumb|right|Figure1: OsDREB148 was rapidly induced by 15 min of exposure to MeJA and was gradually responsive to drought conditions]][1]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> <br /> Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> <br /> GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> <br /> School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> 1. Ju-Seok Seo1, Joungsu Joo2, Min-Jeong Kim1, Yang Do Choi1 et al.(2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.The Plant Journal<br /> Volume 65, Issue 6:907–921<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Expression.gif&diff=169530 2014-05-18T15:22:05Z

<p>Shixing: uploaded a new version of &amp;quot;File:Expression.gif&amp;quot;: OsDREB1A was rapidly induced by 15 min of exposure to MeJA and was gradually responsive to drought conditions.</p> <hr /> <div>OsDREB1A was rapidly induced by 15 min of exposure to MeJA and was gradually responsive to drought conditions</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169529 2014-05-18T15:16:44Z

<p>Shixing: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 is a basic helix-loop-helix protein, which interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signaling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signaling module in the upstream signaling pathway to improve drought tolerance in rice.[1]<br /> <br /> ===Expression===<br /> <br /> OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding.[[Expression.gif]][1]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> <br /> Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> <br /> GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> <br /> School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> 1. Ju-Seok Seo1, Joungsu Joo2, Min-Jeong Kim1, Yang Do Choi1 et al.(2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.The Plant Journal<br /> Volume 65, Issue 6:907–921<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169528 2014-05-18T15:07:59Z

<p>Shixing: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 is a basic helix-loop-helix protein, which interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signaling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signaling module in the upstream signaling pathway to improve drought tolerance in rice.[1]<br /> <br /> ===Expression===<br /> <br /> OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding.[1]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> <br /> Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> <br /> GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> <br /> School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> 1. Ju-Seok Seo1, Joungsu Joo2, Min-Jeong Kim1, Yang Do Choi1 et al.(2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.The Plant Journal<br /> Volume 65, Issue 6:907–921<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169527 2014-05-18T15:06:24Z

<p>Shixing: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 is a basic helix-loop-helix protein, which interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signaling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signaling module in the upstream signaling pathway to improve drought tolerance in rice.[[1]]<br /> <br /> ===Expression===<br /> <br /> OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding.[1]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> <br /> Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> <br /> GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> <br /> School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> 1. Ju-Seok Seo1, Joungsu Joo2, Min-Jeong Kim1, Yang Do Choi1 et al.(2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.The Plant Journal<br /> Volume 65, Issue 6:907–921<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169526 2014-05-18T15:05:19Z

<p>Shixing: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 is a basic helix-loop-helix protein, which interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signaling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signaling module in the upstream signaling pathway to improve drought tolerance in rice.[[Link title]]<br /> <br /> ===Expression===<br /> <br /> OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding.[1]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> <br /> Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> <br /> GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> <br /> School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> 1. Ju-Seok Seo1, Joungsu Joo2, Min-Jeong Kim1, Yang Do Choi1 et al.(2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.The Plant Journal<br /> Volume 65, Issue 6:907–921<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169525 2014-05-18T15:04:25Z

<p>Shixing: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 is a basic helix-loop-helix protein, which interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signaling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signaling module in the upstream signaling pathway to improve drought tolerance in rice.[1]<br /> <br /> ===Expression===<br /> <br /> OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding.[1]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> <br /> Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> <br /> GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> <br /> School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> 1. Ju-Seok Seo1, Joungsu Joo2, Min-Jeong Kim1, Yang Do Choi1 et al.(2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.The Plant Journal<br /> Volume 65, Issue 6:907–921<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169524 2014-05-18T15:03:43Z

<p>Shixing: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 is a basic helix-loop-helix protein, which interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signaling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signaling module in the upstream signaling pathway to improve drought tolerance in rice.<br /> <br /> ===Expression===<br /> <br /> OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> <br /> Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> <br /> GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> <br /> School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> 1. Ju-Seok Seo1, Joungsu Joo2, Min-Jeong Kim1, Yang Do Choi1 et al.(2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.The Plant Journal<br /> Volume 65, Issue 6:907–921<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169523 2014-05-18T15:02:33Z

<p>Shixing: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 is a basic helix-loop-helix protein, which interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signaling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signaling module in the upstream signaling pathway to improve drought tolerance in rice.<br /> <br /> ===Expression===<br /> <br /> OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding.[[Media:C:\Users\sx14\Desktop\expression]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> <br /> Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> <br /> GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> <br /> School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> 1. Ju-Seok Seo1, Joungsu Joo2, Min-Jeong Kim1, Yang Do Choi1 et al.(2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.The Plant Journal<br /> Volume 65, Issue 6:907–921<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169522 2014-05-18T14:57:13Z

<p>Shixing: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 is a basic helix-loop-helix protein, which interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signaling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signaling module in the upstream signaling pathway to improve drought tolerance in rice.<br /> <br /> ===Expression===<br /> <br /> OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding.[[Media:C:\Users\sx14\Desktop\expression]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> <br /> Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> <br /> GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> <br /> School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169521 2014-05-18T14:56:51Z

<p>Shixing: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 is a basic helix-loop-helix protein, which interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signaling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signaling module in the upstream signaling pathway to improve drought tolerance in rice.<br /> <br /> ===Expression===<br /> <br /> OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding.[[Media:C:\Users\sx14\Desktop\expression]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea<br /> Division of Bioscience and Bioinformatics, Myongi University, Yongin 449-728, Korea<br /> GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea<br /> School of Biological Science, Seoul National University, Seoul 151-742, Korea<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0741100&diff=169518 2014-05-18T14:53:20Z

<p>Shixing: /* Annotated Information */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> OsbHLH148 is a basic helix-loop-helix protein, which interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice. The over-expressed OsbHLH148 can cause the up-regulation of some genes involved in stress responses and the jasmonate signaling pathway such as OsDREB and OsJAZ genes. OsbHLH148 acts in the initial response of jasmonates by forming an OsbHLH148–OsJAZ1–OsCOI1 signaling module in the upstream signaling pathway to improve drought tolerance in rice.<br /> <br /> ===Expression===<br /> <br /> OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding.[[Media:C:\Users\sx14\Desktop\expression]]<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os03g0741100|<br /> Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|<br /> Version = NM_001057756.1 GI:115455240 GeneID:4334060|<br /> Length = 2513 bp|<br /> Definition = Oryza sativa Japonica Group Os03g0741100, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|<br /> AP = Chromosome 3:31169988..31172500|<br /> CDS = 31170066..31170542,31170910..31171197,31172163..31172297|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008396:31169988..31172500<br /> source=RiceChromosome03<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacgattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPE QAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGE GEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKG DKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSID SMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQ PDSDAPFPGSKGWTQTSHVQNVF&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;79..555#923..1210#2176..2310#ggaaaccatcgccaaccatcacatctgatcgagaacaagaagaagaagaagaagaagcaagaacagcaagaggaagagatgcaaatggagtcgtactacggcgcgttccacgccgacgaggccgcgttcttcttcccccaccacgtgccggcgtcgccggagctgccgttcggcctcattgcgtcgccggagccggagccggagccggagcaggcggcggcggaggcgaggcagagcgcgttccaggagtacggcggcgcggtgcacgccggagcgccggcggcggcgggggctgtcaccaccggtgggacgaacatccaccggagggtgatggacgtgctgggcaggatgggcggcggcggcggcgggggggagaagggggagggtgaggagatggaggaggaggaggaggtgccgcagcggcggcggcgcgggcagggcgccgacgtggagagcagccgcggtttccgccacatgatgcgcgagcgccagcgccgcgagaagctcagccagagctacgccgacctctacgccatggtctcctctcgctccaaggttcgtcggcctcctctgctcacagcctaagcttatcactctcacttagtactctacttcacttcgccgctcacttcatttcaagctccatttcatctttcacctagattccatcgagaaatgcctaatgtagactgctaatttggccttctttaagctcagattttcgtttaccatcgcattgtcccatttcattggcttcaatttgcctcttcttgatttccccaatgttaatctgaaattcagagcatcttcagctcaaaccaattctgttcagcttcagaatgttcgaaatccccctttaatacctcttaaatggagtgtttaccaaaatcgggctgacaaaaaaccgttcgtgtcactgcagggggacaagaactcgatcgtgcagtcggcggcgatctacatccacgagctgaaggtcgcgagggaccagctgcagaggaggaacgaggagctgaaggcccagatcatgggccacgacgagcagcagccgtgcgtcacggtgcagttcgaggtcgacgagccgtcgtcgtcgatcgactccatgatcgccgcgctccggcggctcaagggcatgagcgtcaaggcgcgcgggatccggtcgagcatgtccgggaacaggctgtggacggagatgaacgtcgagaccacggtgagagctctctctctctctctctctctctctccccccccccccccccccccccccccccccccccccccccttggcttgcaattcttgaaaatttttgcattttttcccctctgaacttagcctacctgccttgttgacagtaactgtcagagttttaattaggttaggaaagatgcagcagttcgtctcctacaagattagcctcattttcgtgttttatcaatacactgtatcttgccttggatagctacggattagattgttacgtacagtagtagataggaaaccgtagtggcaacgggataactggataaggatgaagagttctaatcaacttcagaaatggaagatggaagcatattggttgggagaatttgggctatttttttttcgaataagaagaatttgggctaaattagagaagcatcagagtaacgtttccgaaggaaaagattagtgcagtaattttgctgaactattttatatcctgtccacttgaggtcaagatgcaggaattccactcgaaaccccccaatttgacattagtcaaccgtgtcaagagttcttgtcgaatcttttggtttatgcataaatcatttagcagatcctcaaccccaagaccccaattacaaatagctgtaattagaggtttaattaatcagcaactgttgttagttagcactggtcatccaactgccaaatcaatctttcctcaaaagattattacaagtagttcacaaacatccaaacatgatctttcaaaaggaaacatccaaattccaaacagccataccatattttatactacagagcaacaatcggggccaattacagtccatgcctatccaatattaaactgaaaaagtttattataattataacactagcagttgattaataatagtaagccaattgtacactctaactaacgacgtttaatttcgcatgtgtcattttcacagattgcggcttgtgaagtggaaaaggcagtggaagaggctctcaaggaagtagagaggaaccagcctgacagcgatgccccatttcctggaagcaaaggctggacacagacatctcatgtgcaaaatgtgttttgatcggttttgaaacatgatcctgctgggacccccatttgttcatttttttaaaagcttgtgatggagcaagtggaaaaaaaatctccatatctccttgtcaatgtaccttttttttgtccatgtagatacaatcatacaaataatagcttcctctcttttctacaaggaaaaaagaaagagaagagtacaaaaaagatttaagc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057756.1 RefSeq:Os03g0741100]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 3]]<br /> [[Category:Chromosome 3]]</div>

Shixing https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Expression.gif&diff=169516 2014-05-18T14:51:42Z

<p>Shixing: OsDREB1A was rapidly induced by 15 min of exposure to MeJA and was gradually responsive to drought conditions</p> <hr /> <div>OsDREB1A was rapidly induced by 15 min of exposure to MeJA and was gradually responsive to drought conditions</div>

Shixing