RiceWiki - User contributions [en]

Os03g0327800

2014-07-01T12:52:54Z

Suchao13: /* References */

Please input one-sentence summary here.

==Annotated Information==
===Function===

*Os03g0327800 encoding a Oryza sativa NAC-like, activated by apetala3/pistillata (OsNAP). Overexpression of OsNAP significantly promoted senescence, whereas knockdown of OsNAP produced a marked delay of senescence, confirming the role of this gene in the development of rice senescence.

*ABA Participates in Leaf Senescence and the expression of OsNAP can be induced by ABA. ABA-mediated leaf senescence might be dependent on the modulation of OsNAP expression, which, in turn, either directly or indirectly regulates the expression of senescence-associated genes (SAGs). Also, this is a feedback mechanism between ABA and OsNAP, OsNAP can inhibit the expression of key ABA biosynthesis genes.

[[File:nap1.jpg]]

===Expression===
* OsNAP is expressed preferentially in the leaf blade, leaf sheath, and endosperm with a low, but still detectable, level of expression in the root, culm,
and young panicle.

[[File:nap2.jpg]]

*OsNAP transcripts were higher in old, senescing leaves than in young, green leaves.

[[File:nap3.jpg]]

*OsNAP expression in flag leaves showed that, although the number of OsNAP transcripts increased gradually before heading, it increased dramatically with the beginning of grain filling.(The red arrow indicates the flowering time)

[[File:nap4.jpg]]

*OsNAP expression decreased gradually from the tip to the base of a fully expanded leaf.

[[File:nap5.jpg]]

*ABA can induce the expression of OsNAP, and in ABA biosynthetic mutants (aba1 and aba2) the expression is decreased.

[[File:nap6.jpg]]

===Mutation and Phenotype===
ps1-D is a dominant mutation caused by the T-DNA insertion.

[[File:nap7.jpg]]

PS1 contains a typical NAC structure at the N terminus. It belongs to the Va (1)/NAP subfamily of NAC proteins, which has three members in Arabidopsis, six members in rice, and five members in maize. Detailed domain analysis showed that the C-terminal region (amino acids 181–392) possessed high transcriptional activation activity,fragment containing subdomains 3 (amino acids 64–100) and 4 (amino acids 100–142) of the NAC motif showed stronger repression activity, whereas subdomains 1 and 2 (amino acids 1–64) and 5 (amino acids 157–181) exhibited weaker repression activity.

[[File:nap8.jpg]]

after grain filling, ps1-D mutants had an accelerated yellowing phenotype, displaying senescence 7–10 d earlier than the wild-type plants. In addition, after flowering, ps1-D mutants also exhibited faster chlorophyll degradation than the wild type plants.

[[File:nap9.jpg]]

OsNAP RNAi lines can delay the senescence.

[[File:nap10.jpg]]

===other information===

*Primers used in this study for molecular cloning of PS1

[[File:nap11.jpg]]

*Primers used to generate DNA constructs

[[File:nap12.jpg]]
[[File:nap13.jpg]]

==Labs working on this gene==
*State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology,
Chinese Academy of Sciences

*University of Chinese Academy of Sciences, Beijing 100049, China; and cSchool of Agriculture and Food Science, Zhejiang Agricultural and Forestry University, Hangzhou

==References==
Chengzhen Liang, Yiqin Wang, Yana Zhu, et al. OsNAP connects abscisic acid and leaf senescence by fine-tuning abscisic acid biosynthesis and directly
targeting senescence-associated genes in rice. PNAS, 2014

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0327800|
Description = No apical meristem (NAM) protein domain containing protein|
Version = NM_001056532.2 GI:297600855 GeneID:4332717|
Length = 2417 bp|
Definition = Oryza sativa Japonica Group Os03g0327800, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:12024636..12027052|
CDS = 12026216..12026613,12026708..12026873|
GCID = <gbrowseImage1>
name=NC_008396:12024636..12027052
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:12024636..12027052
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggttctgtcgaacccggcgatgctgccgccggggttccggttccacccgacggacgaggagctgatcgtgcactacctccgcaaccgggccgcctcctcgccgtgccccgtctccatcatcgccgacgtcgacatctacaagttcgatccgtgggacctcccatccaaggagaattacggggacagggagtggtacttcttcagcccgagggaccgcaagtacccgaacgggatccggccgaaccgcgccgcgggctccggctactggaaggccaccggcaccgacaagcccatccacagcagcggcggcgcggcgaccaacgagagcgtcggcgtcaagaaggcgctcgtcttctacaagggccgcccgcccaagggcaccaagaccaactggatcatgcacgagtaccgcctcgccgccgcagacgcccacgccgccaacacctaccgccccatgaagttccgcaacacctccatgagggtaagcaagctccatctacgttacctagcagccagattcaaattatctaaaccaaacaacttaattatgaacaaaccctag</cdnaseq>|
AA = <aaseq>MVLSNPAMLPPGFRFHPTDEELIVHYLRNRAASSPCPVSIIADV DIYKFDPWDLPSKENYGDREWYFFSPRDRKYPNGIRPNRAAGSGYWKATGTDKPIHSS GGAATNESVGVKKALVFYKGRPPKGTKTNWIMHEYRLAAADAHAANTYRPMKFRNTSM RVSKLHLRYLAARFKLSKPNNLIMNKP</aaseq>|
DNA = <dnaseqindica>440..837#180..345#aaattcgagccctcctccatctctcgcctcctcttgatacaagctcgaccctgatagatctcatagatcatagctgagaggggatcgagtggcttgatcttggaagtgagcagctatagctagataggttttgcttgtgcagggaggtgtgtgttgtgtgtgggtgactgccggagacgatggttctgtcgaacccggcgatgctgccgccggggttccggttccacccgacggacgaggagctgatcgtgcactacctccgcaaccgggccgcctcctcgccgtgccccgtctccatcatcgccgacgtcgacatctacaagttcgatccgtgggacctcccatgttagtacccaccctcacagctcgccgcgatcgatcgatcactgatttgcaattaatttcgttggtgatcgacttgtgttttgatttgcatcagccaaggagaattacggggacagggagtggtacttcttcagcccgagggaccgcaagtacccgaacgggatccggccgaaccgcgccgcgggctccggctactggaaggccaccggcaccgacaagcccatccacagcagcggcggcgcggcgaccaacgagagcgtcggcgtcaagaaggcgctcgtcttctacaagggccgcccgcccaagggcaccaagaccaactggatcatgcacgagtaccgcctcgccgccgcagacgcccacgccgccaacacctaccgccccatgaagttccgcaacacctccatgagggtaagcaagctccatctacgttacctagcagccagattcaaattatctaaaccaaacaacttaattatgaacaaaccctagcagctggatcttgctatcgatggagtagagagtagatcgtctattattcatctctgtctgatgtgctagtgcacttgcacagttgcacaccgtggtttttttgggtggttgtttctgatgatcgatacgtgttcgtcggcacgtcagtattatcgcatcggtttgtgccttgccaatggacgcaactcgctgcttttagtgtgtctgttttaaagagggtgttctttaggcaatacttggcatggttgcgcaagtcaatagatccgacaatgatagccacgatgcgtacgtgttgatgactggttcatctctgcatatgcgcgcatgcataccccctgaaattaatcccttctatttttgatactcttaatttcttaatcatatagtatatgcatccgtgtgattgatcgatcgatgtgcgtggatttttgtgcagctggatgactgggtgctgtgccggatctacaagaagagcagccacgcgtcgccgctggccgtgccgccgctctccgaccacgagcaggacgagccgtgcgccctggaggagaacgcgccgctgtacgcgccgtcgtcgtcgagcgccgcatccatgatcttgcagggcgctgccgccggcgccttcccgtcgctgcacgccgccgccgccgctacgcagaggacggcgatgcagaagatcccttccatttccgacctgctcaacgagtactcgctgtcgcagctcttcgacgacggcggcgccgccgccgccgcccccctgcaggagatggcgaggcagcccgaccaccaccaccaccaacaacaacaacacgccctctttggccaccccgtcatgaaccatttcatcgcgaacaacagcatggttcagctcgcgcacctggacccgtcctcctctgcggccgcctcgacgtcggcaggcgccgtcgtcgagccgccggccgtcaccgggaagcgcaagagatcatcggacggaggcgagccgacgatccaggcgctgcctcctgctgccgcggcggccaagaagccgaacggttcatgcgttggtgcaactttccaaataggcagcgccttgcagggatcgtcactgggactgagccaccagatgctgctccactctaacatggggatgaactgatagctcgttcttcgtacataataccacagcctgattaaacactgaacataattgcacatggcgaattgatgatttgaacagtgaggtctatttcgaaagcaaataggtccaaaacgatagatttaaatagtaaatatattgggaaagaaattgtacttaatttgtacttgtgtggactagctaggtcgtccgatcatgcatagctatatagtttgtggtgtgtatacattttttctcatggtactatactactgtgtactcaagctagctcagtactaaagattaattaagctagataggggttgataagactttgagatagacccactagaagtctggaacatgagatcaaggagaatgatgtcaagatcataaagccggatttatagctcatattcatgtaaatcaatcaataagtgattctttaatggcagccactcattttttt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001056532.2 RefSeq:Os03g0327800]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

Os03g0327800

2014-07-01T12:51:02Z

Suchao13: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==
===Function===

*Os03g0327800 encoding a Oryza sativa NAC-like, activated by apetala3/pistillata (OsNAP). Overexpression of OsNAP significantly promoted senescence, whereas knockdown of OsNAP produced a marked delay of senescence, confirming the role of this gene in the development of rice senescence.

*ABA Participates in Leaf Senescence and the expression of OsNAP can be induced by ABA. ABA-mediated leaf senescence might be dependent on the modulation of OsNAP expression, which, in turn, either directly or indirectly regulates the expression of senescence-associated genes (SAGs). Also, this is a feedback mechanism between ABA and OsNAP, OsNAP can inhibit the expression of key ABA biosynthesis genes.

[[File:nap1.jpg]]

===Expression===
* OsNAP is expressed preferentially in the leaf blade, leaf sheath, and endosperm with a low, but still detectable, level of expression in the root, culm,
and young panicle.

[[File:nap2.jpg]]

*OsNAP transcripts were higher in old, senescing leaves than in young, green leaves.

[[File:nap3.jpg]]

*OsNAP expression in flag leaves showed that, although the number of OsNAP transcripts increased gradually before heading, it increased dramatically with the beginning of grain filling.(The red arrow indicates the flowering time)

[[File:nap4.jpg]]

*OsNAP expression decreased gradually from the tip to the base of a fully expanded leaf.

[[File:nap5.jpg]]

*ABA can induce the expression of OsNAP, and in ABA biosynthetic mutants (aba1 and aba2) the expression is decreased.

[[File:nap6.jpg]]

===Mutation and Phenotype===
ps1-D is a dominant mutation caused by the T-DNA insertion.

[[File:nap7.jpg]]

PS1 contains a typical NAC structure at the N terminus. It belongs to the Va (1)/NAP subfamily of NAC proteins, which has three members in Arabidopsis, six members in rice, and five members in maize. Detailed domain analysis showed that the C-terminal region (amino acids 181–392) possessed high transcriptional activation activity,fragment containing subdomains 3 (amino acids 64–100) and 4 (amino acids 100–142) of the NAC motif showed stronger repression activity, whereas subdomains 1 and 2 (amino acids 1–64) and 5 (amino acids 157–181) exhibited weaker repression activity.

[[File:nap8.jpg]]

after grain filling, ps1-D mutants had an accelerated yellowing phenotype, displaying senescence 7–10 d earlier than the wild-type plants. In addition, after flowering, ps1-D mutants also exhibited faster chlorophyll degradation than the wild type plants.

[[File:nap9.jpg]]

OsNAP RNAi lines can delay the senescence.

[[File:nap10.jpg]]

===other information===

*Primers used in this study for molecular cloning of PS1

[[File:nap11.jpg]]

*Primers used to generate DNA constructs

[[File:nap12.jpg]]
[[File:nap13.jpg]]

==Labs working on this gene==
*State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology,
Chinese Academy of Sciences

*University of Chinese Academy of Sciences, Beijing 100049, China; and cSchool of Agriculture and Food Science, Zhejiang Agricultural and Forestry University, Hangzhou

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0327800|
Description = No apical meristem (NAM) protein domain containing protein|
Version = NM_001056532.2 GI:297600855 GeneID:4332717|
Length = 2417 bp|
Definition = Oryza sativa Japonica Group Os03g0327800, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:12024636..12027052|
CDS = 12026216..12026613,12026708..12026873|
GCID = <gbrowseImage1>
name=NC_008396:12024636..12027052
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:12024636..12027052
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggttctgtcgaacccggcgatgctgccgccggggttccggttccacccgacggacgaggagctgatcgtgcactacctccgcaaccgggccgcctcctcgccgtgccccgtctccatcatcgccgacgtcgacatctacaagttcgatccgtgggacctcccatccaaggagaattacggggacagggagtggtacttcttcagcccgagggaccgcaagtacccgaacgggatccggccgaaccgcgccgcgggctccggctactggaaggccaccggcaccgacaagcccatccacagcagcggcggcgcggcgaccaacgagagcgtcggcgtcaagaaggcgctcgtcttctacaagggccgcccgcccaagggcaccaagaccaactggatcatgcacgagtaccgcctcgccgccgcagacgcccacgccgccaacacctaccgccccatgaagttccgcaacacctccatgagggtaagcaagctccatctacgttacctagcagccagattcaaattatctaaaccaaacaacttaattatgaacaaaccctag</cdnaseq>|
AA = <aaseq>MVLSNPAMLPPGFRFHPTDEELIVHYLRNRAASSPCPVSIIADV DIYKFDPWDLPSKENYGDREWYFFSPRDRKYPNGIRPNRAAGSGYWKATGTDKPIHSS GGAATNESVGVKKALVFYKGRPPKGTKTNWIMHEYRLAAADAHAANTYRPMKFRNTSM RVSKLHLRYLAARFKLSKPNNLIMNKP</aaseq>|
DNA = <dnaseqindica>440..837#180..345#aaattcgagccctcctccatctctcgcctcctcttgatacaagctcgaccctgatagatctcatagatcatagctgagaggggatcgagtggcttgatcttggaagtgagcagctatagctagataggttttgcttgtgcagggaggtgtgtgttgtgtgtgggtgactgccggagacgatggttctgtcgaacccggcgatgctgccgccggggttccggttccacccgacggacgaggagctgatcgtgcactacctccgcaaccgggccgcctcctcgccgtgccccgtctccatcatcgccgacgtcgacatctacaagttcgatccgtgggacctcccatgttagtacccaccctcacagctcgccgcgatcgatcgatcactgatttgcaattaatttcgttggtgatcgacttgtgttttgatttgcatcagccaaggagaattacggggacagggagtggtacttcttcagcccgagggaccgcaagtacccgaacgggatccggccgaaccgcgccgcgggctccggctactggaaggccaccggcaccgacaagcccatccacagcagcggcggcgcggcgaccaacgagagcgtcggcgtcaagaaggcgctcgtcttctacaagggccgcccgcccaagggcaccaagaccaactggatcatgcacgagtaccgcctcgccgccgcagacgcccacgccgccaacacctaccgccccatgaagttccgcaacacctccatgagggtaagcaagctccatctacgttacctagcagccagattcaaattatctaaaccaaacaacttaattatgaacaaaccctagcagctggatcttgctatcgatggagtagagagtagatcgtctattattcatctctgtctgatgtgctagtgcacttgcacagttgcacaccgtggtttttttgggtggttgtttctgatgatcgatacgtgttcgtcggcacgtcagtattatcgcatcggtttgtgccttgccaatggacgcaactcgctgcttttagtgtgtctgttttaaagagggtgttctttaggcaatacttggcatggttgcgcaagtcaatagatccgacaatgatagccacgatgcgtacgtgttgatgactggttcatctctgcatatgcgcgcatgcataccccctgaaattaatcccttctatttttgatactcttaatttcttaatcatatagtatatgcatccgtgtgattgatcgatcgatgtgcgtggatttttgtgcagctggatgactgggtgctgtgccggatctacaagaagagcagccacgcgtcgccgctggccgtgccgccgctctccgaccacgagcaggacgagccgtgcgccctggaggagaacgcgccgctgtacgcgccgtcgtcgtcgagcgccgcatccatgatcttgcagggcgctgccgccggcgccttcccgtcgctgcacgccgccgccgccgctacgcagaggacggcgatgcagaagatcccttccatttccgacctgctcaacgagtactcgctgtcgcagctcttcgacgacggcggcgccgccgccgccgcccccctgcaggagatggcgaggcagcccgaccaccaccaccaccaacaacaacaacacgccctctttggccaccccgtcatgaaccatttcatcgcgaacaacagcatggttcagctcgcgcacctggacccgtcctcctctgcggccgcctcgacgtcggcaggcgccgtcgtcgagccgccggccgtcaccgggaagcgcaagagatcatcggacggaggcgagccgacgatccaggcgctgcctcctgctgccgcggcggccaagaagccgaacggttcatgcgttggtgcaactttccaaataggcagcgccttgcagggatcgtcactgggactgagccaccagatgctgctccactctaacatggggatgaactgatagctcgttcttcgtacataataccacagcctgattaaacactgaacataattgcacatggcgaattgatgatttgaacagtgaggtctatttcgaaagcaaataggtccaaaacgatagatttaaatagtaaatatattgggaaagaaattgtacttaatttgtacttgtgtggactagctaggtcgtccgatcatgcatagctatatagtttgtggtgtgtatacattttttctcatggtactatactactgtgtactcaagctagctcagtactaaagattaattaagctagataggggttgataagactttgagatagacccactagaagtctggaacatgagatcaaggagaatgatgtcaagatcataaagccggatttatagctcatattcatgtaaatcaatcaataagtgattctttaatggcagccactcattttttt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001056532.2 RefSeq:Os03g0327800]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

File:Nap13.jpg

2014-07-01T12:49:06Z

Suchao13:

File:Nap12.jpg

2014-07-01T12:48:45Z

Suchao13:

File:Nap11.jpg

2014-07-01T12:48:24Z

Suchao13:

Os03g0327800

2014-07-01T12:48:03Z

Suchao13: /* other information */

Please input one-sentence summary here.

==Annotated Information==
===Function===

*Os03g0327800 encoding a Oryza sativa NAC-like, activated by apetala3/pistillata (OsNAP). Overexpression of OsNAP significantly promoted senescence, whereas knockdown of OsNAP produced a marked delay of senescence, confirming the role of this gene in the development of rice senescence.

*ABA Participates in Leaf Senescence and the expression of OsNAP can be induced by ABA. ABA-mediated leaf senescence might be dependent on the modulation of OsNAP expression, which, in turn, either directly or indirectly regulates the expression of senescence-associated genes (SAGs). Also, this is a feedback mechanism between ABA and OsNAP, OsNAP can inhibit the expression of key ABA biosynthesis genes.

[[File:nap1.jpg]]

===Expression===
* OsNAP is expressed preferentially in the leaf blade, leaf sheath, and endosperm with a low, but still detectable, level of expression in the root, culm,
and young panicle.

[[File:nap2.jpg]]

*OsNAP transcripts were higher in old, senescing leaves than in young, green leaves.

[[File:nap3.jpg]]

*OsNAP expression in flag leaves showed that, although the number of OsNAP transcripts increased gradually before heading, it increased dramatically with the beginning of grain filling.(The red arrow indicates the flowering time)

[[File:nap4.jpg]]

*OsNAP expression decreased gradually from the tip to the base of a fully expanded leaf.

[[File:nap5.jpg]]

*ABA can induce the expression of OsNAP, and in ABA biosynthetic mutants (aba1 and aba2) the expression is decreased.

[[File:nap6.jpg]]

===Mutation and Phenotype===
ps1-D is a dominant mutation caused by the T-DNA insertion.

[[File:nap7.jpg]]

PS1 contains a typical NAC structure at the N terminus. It belongs to the Va (1)/NAP subfamily of NAC proteins, which has three members in Arabidopsis, six members in rice, and five members in maize. Detailed domain analysis showed that the C-terminal region (amino acids 181–392) possessed high transcriptional activation activity,fragment containing subdomains 3 (amino acids 64–100) and 4 (amino acids 100–142) of the NAC motif showed stronger repression activity, whereas subdomains 1 and 2 (amino acids 1–64) and 5 (amino acids 157–181) exhibited weaker repression activity.

[[File:nap8.jpg]]

after grain filling, ps1-D mutants had an accelerated yellowing phenotype, displaying senescence 7–10 d earlier than the wild-type plants. In addition, after flowering, ps1-D mutants also exhibited faster chlorophyll degradation than the wild type plants.

[[File:nap9.jpg]]

OsNAP RNAi lines can delay the senescence.

[[File:nap10.jpg]]

===other information===

*Primers used in this study for molecular cloning of PS1

[[File:nap11.jpg]]

*Primers used to generate DNA constructs

[[File:nap12.jpg]]
[[File:nap13.jpg]]

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0327800|
Description = No apical meristem (NAM) protein domain containing protein|
Version = NM_001056532.2 GI:297600855 GeneID:4332717|
Length = 2417 bp|
Definition = Oryza sativa Japonica Group Os03g0327800, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:12024636..12027052|
CDS = 12026216..12026613,12026708..12026873|
GCID = <gbrowseImage1>
name=NC_008396:12024636..12027052
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:12024636..12027052
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggttctgtcgaacccggcgatgctgccgccggggttccggttccacccgacggacgaggagctgatcgtgcactacctccgcaaccgggccgcctcctcgccgtgccccgtctccatcatcgccgacgtcgacatctacaagttcgatccgtgggacctcccatccaaggagaattacggggacagggagtggtacttcttcagcccgagggaccgcaagtacccgaacgggatccggccgaaccgcgccgcgggctccggctactggaaggccaccggcaccgacaagcccatccacagcagcggcggcgcggcgaccaacgagagcgtcggcgtcaagaaggcgctcgtcttctacaagggccgcccgcccaagggcaccaagaccaactggatcatgcacgagtaccgcctcgccgccgcagacgcccacgccgccaacacctaccgccccatgaagttccgcaacacctccatgagggtaagcaagctccatctacgttacctagcagccagattcaaattatctaaaccaaacaacttaattatgaacaaaccctag</cdnaseq>|
AA = <aaseq>MVLSNPAMLPPGFRFHPTDEELIVHYLRNRAASSPCPVSIIADV DIYKFDPWDLPSKENYGDREWYFFSPRDRKYPNGIRPNRAAGSGYWKATGTDKPIHSS GGAATNESVGVKKALVFYKGRPPKGTKTNWIMHEYRLAAADAHAANTYRPMKFRNTSM RVSKLHLRYLAARFKLSKPNNLIMNKP</aaseq>|
DNA = <dnaseqindica>440..837#180..345#aaattcgagccctcctccatctctcgcctcctcttgatacaagctcgaccctgatagatctcatagatcatagctgagaggggatcgagtggcttgatcttggaagtgagcagctatagctagataggttttgcttgtgcagggaggtgtgtgttgtgtgtgggtgactgccggagacgatggttctgtcgaacccggcgatgctgccgccggggttccggttccacccgacggacgaggagctgatcgtgcactacctccgcaaccgggccgcctcctcgccgtgccccgtctccatcatcgccgacgtcgacatctacaagttcgatccgtgggacctcccatgttagtacccaccctcacagctcgccgcgatcgatcgatcactgatttgcaattaatttcgttggtgatcgacttgtgttttgatttgcatcagccaaggagaattacggggacagggagtggtacttcttcagcccgagggaccgcaagtacccgaacgggatccggccgaaccgcgccgcgggctccggctactggaaggccaccggcaccgacaagcccatccacagcagcggcggcgcggcgaccaacgagagcgtcggcgtcaagaaggcgctcgtcttctacaagggccgcccgcccaagggcaccaagaccaactggatcatgcacgagtaccgcctcgccgccgcagacgcccacgccgccaacacctaccgccccatgaagttccgcaacacctccatgagggtaagcaagctccatctacgttacctagcagccagattcaaattatctaaaccaaacaacttaattatgaacaaaccctagcagctggatcttgctatcgatggagtagagagtagatcgtctattattcatctctgtctgatgtgctagtgcacttgcacagttgcacaccgtggtttttttgggtggttgtttctgatgatcgatacgtgttcgtcggcacgtcagtattatcgcatcggtttgtgccttgccaatggacgcaactcgctgcttttagtgtgtctgttttaaagagggtgttctttaggcaatacttggcatggttgcgcaagtcaatagatccgacaatgatagccacgatgcgtacgtgttgatgactggttcatctctgcatatgcgcgcatgcataccccctgaaattaatcccttctatttttgatactcttaatttcttaatcatatagtatatgcatccgtgtgattgatcgatcgatgtgcgtggatttttgtgcagctggatgactgggtgctgtgccggatctacaagaagagcagccacgcgtcgccgctggccgtgccgccgctctccgaccacgagcaggacgagccgtgcgccctggaggagaacgcgccgctgtacgcgccgtcgtcgtcgagcgccgcatccatgatcttgcagggcgctgccgccggcgccttcccgtcgctgcacgccgccgccgccgctacgcagaggacggcgatgcagaagatcccttccatttccgacctgctcaacgagtactcgctgtcgcagctcttcgacgacggcggcgccgccgccgccgcccccctgcaggagatggcgaggcagcccgaccaccaccaccaccaacaacaacaacacgccctctttggccaccccgtcatgaaccatttcatcgcgaacaacagcatggttcagctcgcgcacctggacccgtcctcctctgcggccgcctcgacgtcggcaggcgccgtcgtcgagccgccggccgtcaccgggaagcgcaagagatcatcggacggaggcgagccgacgatccaggcgctgcctcctgctgccgcggcggccaagaagccgaacggttcatgcgttggtgcaactttccaaataggcagcgccttgcagggatcgtcactgggactgagccaccagatgctgctccactctaacatggggatgaactgatagctcgttcttcgtacataataccacagcctgattaaacactgaacataattgcacatggcgaattgatgatttgaacagtgaggtctatttcgaaagcaaataggtccaaaacgatagatttaaatagtaaatatattgggaaagaaattgtacttaatttgtacttgtgtggactagctaggtcgtccgatcatgcatagctatatagtttgtggtgtgtatacattttttctcatggtactatactactgtgtactcaagctagctcagtactaaagattaattaagctagataggggttgataagactttgagatagacccactagaagtctggaacatgagatcaaggagaatgatgtcaagatcataaagccggatttatagctcatattcatgtaaatcaatcaataagtgattctttaatggcagccactcattttttt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001056532.2 RefSeq:Os03g0327800]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

File:Nap10.jpg

2014-07-01T12:43:00Z

Suchao13:

File:Nap9.jpg

2014-07-01T12:42:40Z

Suchao13:

File:Nap8.jpg

2014-07-01T12:42:18Z

Suchao13:

File:Nap7.jpg

2014-07-01T12:41:56Z

Suchao13:

Os03g0327800

2014-07-01T12:40:49Z

Suchao13: /* Mutation and Phenotype */

Please input one-sentence summary here.

==Annotated Information==
===Function===

*Os03g0327800 encoding a Oryza sativa NAC-like, activated by apetala3/pistillata (OsNAP). Overexpression of OsNAP significantly promoted senescence, whereas knockdown of OsNAP produced a marked delay of senescence, confirming the role of this gene in the development of rice senescence.

*ABA Participates in Leaf Senescence and the expression of OsNAP can be induced by ABA. ABA-mediated leaf senescence might be dependent on the modulation of OsNAP expression, which, in turn, either directly or indirectly regulates the expression of senescence-associated genes (SAGs). Also, this is a feedback mechanism between ABA and OsNAP, OsNAP can inhibit the expression of key ABA biosynthesis genes.

[[File:nap1.jpg]]

===Expression===
* OsNAP is expressed preferentially in the leaf blade, leaf sheath, and endosperm with a low, but still detectable, level of expression in the root, culm,
and young panicle.

[[File:nap2.jpg]]

*OsNAP transcripts were higher in old, senescing leaves than in young, green leaves.

[[File:nap3.jpg]]

*OsNAP expression in flag leaves showed that, although the number of OsNAP transcripts increased gradually before heading, it increased dramatically with the beginning of grain filling.(The red arrow indicates the flowering time)

[[File:nap4.jpg]]

*OsNAP expression decreased gradually from the tip to the base of a fully expanded leaf.

[[File:nap5.jpg]]

*ABA can induce the expression of OsNAP, and in ABA biosynthetic mutants (aba1 and aba2) the expression is decreased.

[[File:nap6.jpg]]

===Mutation and Phenotype===
ps1-D is a dominant mutation caused by the T-DNA insertion.

[[File:nap7.jpg]]

PS1 contains a typical NAC structure at the N terminus. It belongs to the Va (1)/NAP subfamily of NAC proteins, which has three members in Arabidopsis, six members in rice, and five members in maize. Detailed domain analysis showed that the C-terminal region (amino acids 181–392) possessed high transcriptional activation activity,fragment containing subdomains 3 (amino acids 64–100) and 4 (amino acids 100–142) of the NAC motif showed stronger repression activity, whereas subdomains 1 and 2 (amino acids 1–64) and 5 (amino acids 157–181) exhibited weaker repression activity.

[[File:nap8.jpg]]

after grain filling, ps1-D mutants had an accelerated yellowing phenotype, displaying senescence 7–10 d earlier than the wild-type plants. In addition, after flowering, ps1-D mutants also exhibited faster chlorophyll degradation than the wild type plants.

[[File:nap9.jpg]]

OsNAP RNAi lines can delay the senescence.

[[File:nap10.jpg]]

===other information===

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0327800|
Description = No apical meristem (NAM) protein domain containing protein|
Version = NM_001056532.2 GI:297600855 GeneID:4332717|
Length = 2417 bp|
Definition = Oryza sativa Japonica Group Os03g0327800, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:12024636..12027052|
CDS = 12026216..12026613,12026708..12026873|
GCID = <gbrowseImage1>
name=NC_008396:12024636..12027052
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:12024636..12027052
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggttctgtcgaacccggcgatgctgccgccggggttccggttccacccgacggacgaggagctgatcgtgcactacctccgcaaccgggccgcctcctcgccgtgccccgtctccatcatcgccgacgtcgacatctacaagttcgatccgtgggacctcccatccaaggagaattacggggacagggagtggtacttcttcagcccgagggaccgcaagtacccgaacgggatccggccgaaccgcgccgcgggctccggctactggaaggccaccggcaccgacaagcccatccacagcagcggcggcgcggcgaccaacgagagcgtcggcgtcaagaaggcgctcgtcttctacaagggccgcccgcccaagggcaccaagaccaactggatcatgcacgagtaccgcctcgccgccgcagacgcccacgccgccaacacctaccgccccatgaagttccgcaacacctccatgagggtaagcaagctccatctacgttacctagcagccagattcaaattatctaaaccaaacaacttaattatgaacaaaccctag</cdnaseq>|
AA = <aaseq>MVLSNPAMLPPGFRFHPTDEELIVHYLRNRAASSPCPVSIIADV DIYKFDPWDLPSKENYGDREWYFFSPRDRKYPNGIRPNRAAGSGYWKATGTDKPIHSS GGAATNESVGVKKALVFYKGRPPKGTKTNWIMHEYRLAAADAHAANTYRPMKFRNTSM RVSKLHLRYLAARFKLSKPNNLIMNKP</aaseq>|
DNA = <dnaseqindica>440..837#180..345#aaattcgagccctcctccatctctcgcctcctcttgatacaagctcgaccctgatagatctcatagatcatagctgagaggggatcgagtggcttgatcttggaagtgagcagctatagctagataggttttgcttgtgcagggaggtgtgtgttgtgtgtgggtgactgccggagacgatggttctgtcgaacccggcgatgctgccgccggggttccggttccacccgacggacgaggagctgatcgtgcactacctccgcaaccgggccgcctcctcgccgtgccccgtctccatcatcgccgacgtcgacatctacaagttcgatccgtgggacctcccatgttagtacccaccctcacagctcgccgcgatcgatcgatcactgatttgcaattaatttcgttggtgatcgacttgtgttttgatttgcatcagccaaggagaattacggggacagggagtggtacttcttcagcccgagggaccgcaagtacccgaacgggatccggccgaaccgcgccgcgggctccggctactggaaggccaccggcaccgacaagcccatccacagcagcggcggcgcggcgaccaacgagagcgtcggcgtcaagaaggcgctcgtcttctacaagggccgcccgcccaagggcaccaagaccaactggatcatgcacgagtaccgcctcgccgccgcagacgcccacgccgccaacacctaccgccccatgaagttccgcaacacctccatgagggtaagcaagctccatctacgttacctagcagccagattcaaattatctaaaccaaacaacttaattatgaacaaaccctagcagctggatcttgctatcgatggagtagagagtagatcgtctattattcatctctgtctgatgtgctagtgcacttgcacagttgcacaccgtggtttttttgggtggttgtttctgatgatcgatacgtgttcgtcggcacgtcagtattatcgcatcggtttgtgccttgccaatggacgcaactcgctgcttttagtgtgtctgttttaaagagggtgttctttaggcaatacttggcatggttgcgcaagtcaatagatccgacaatgatagccacgatgcgtacgtgttgatgactggttcatctctgcatatgcgcgcatgcataccccctgaaattaatcccttctatttttgatactcttaatttcttaatcatatagtatatgcatccgtgtgattgatcgatcgatgtgcgtggatttttgtgcagctggatgactgggtgctgtgccggatctacaagaagagcagccacgcgtcgccgctggccgtgccgccgctctccgaccacgagcaggacgagccgtgcgccctggaggagaacgcgccgctgtacgcgccgtcgtcgtcgagcgccgcatccatgatcttgcagggcgctgccgccggcgccttcccgtcgctgcacgccgccgccgccgctacgcagaggacggcgatgcagaagatcccttccatttccgacctgctcaacgagtactcgctgtcgcagctcttcgacgacggcggcgccgccgccgccgcccccctgcaggagatggcgaggcagcccgaccaccaccaccaccaacaacaacaacacgccctctttggccaccccgtcatgaaccatttcatcgcgaacaacagcatggttcagctcgcgcacctggacccgtcctcctctgcggccgcctcgacgtcggcaggcgccgtcgtcgagccgccggccgtcaccgggaagcgcaagagatcatcggacggaggcgagccgacgatccaggcgctgcctcctgctgccgcggcggccaagaagccgaacggttcatgcgttggtgcaactttccaaataggcagcgccttgcagggatcgtcactgggactgagccaccagatgctgctccactctaacatggggatgaactgatagctcgttcttcgtacataataccacagcctgattaaacactgaacataattgcacatggcgaattgatgatttgaacagtgaggtctatttcgaaagcaaataggtccaaaacgatagatttaaatagtaaatatattgggaaagaaattgtacttaatttgtacttgtgtggactagctaggtcgtccgatcatgcatagctatatagtttgtggtgtgtatacattttttctcatggtactatactactgtgtactcaagctagctcagtactaaagattaattaagctagataggggttgataagactttgagatagacccactagaagtctggaacatgagatcaaggagaatgatgtcaagatcataaagccggatttatagctcatattcatgtaaatcaatcaataagtgattctttaatggcagccactcattttttt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001056532.2 RefSeq:Os03g0327800]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

File:Nap6.jpg

2014-07-01T12:28:01Z

Suchao13:

File:Nap5.jpg

2014-07-01T12:27:37Z

Suchao13:

File:Nap4.jpg

2014-07-01T12:27:16Z

Suchao13:

File:Nap3.jpg

2014-07-01T12:14:26Z

Suchao13:

File:Nap2.jpg

2014-07-01T12:14:08Z

Suchao13:

Os03g0327800

2014-07-01T12:11:08Z

Suchao13: /* Expression */

Please input one-sentence summary here.

==Annotated Information==
===Function===

*Os03g0327800 encoding a Oryza sativa NAC-like, activated by apetala3/pistillata (OsNAP). Overexpression of OsNAP significantly promoted senescence, whereas knockdown of OsNAP produced a marked delay of senescence, confirming the role of this gene in the development of rice senescence.

*ABA Participates in Leaf Senescence and the expression of OsNAP can be induced by ABA. ABA-mediated leaf senescence might be dependent on the modulation of OsNAP expression, which, in turn, either directly or indirectly regulates the expression of senescence-associated genes (SAGs). Also, this is a feedback mechanism between ABA and OsNAP, OsNAP can inhibit the expression of key ABA biosynthesis genes.

[[File:nap1.jpg]]

===Expression===
* OsNAP is expressed preferentially in the leaf blade, leaf sheath, and endosperm with a low, but still detectable, level of expression in the root, culm,
and young panicle.

[[File:nap2.jpg]]

*OsNAP transcripts were higher in old, senescing leaves than in young, green leaves.

[[File:nap3.jpg]]

*OsNAP expression in flag leaves showed that, although the number of OsNAP transcripts increased gradually before heading, it increased dramatically with the beginning of grain filling.(The red arrow indicates the flowering time)

[[File:nap4.jpg]]

*OsNAP expression decreased gradually from the tip to the base of a fully expanded leaf.

[[File:nap5.jpg]]

*ABA can induce the expression of OsNAP, and in ABA biosynthetic mutants (aba1 and aba2) the expression is decreased.

[[File:nap6.jpg]]

===Mutation and Phenotype===
Please input evolution information here.

===other information===

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0327800|
Description = No apical meristem (NAM) protein domain containing protein|
Version = NM_001056532.2 GI:297600855 GeneID:4332717|
Length = 2417 bp|
Definition = Oryza sativa Japonica Group Os03g0327800, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:12024636..12027052|
CDS = 12026216..12026613,12026708..12026873|
GCID = <gbrowseImage1>
name=NC_008396:12024636..12027052
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:12024636..12027052
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggttctgtcgaacccggcgatgctgccgccggggttccggttccacccgacggacgaggagctgatcgtgcactacctccgcaaccgggccgcctcctcgccgtgccccgtctccatcatcgccgacgtcgacatctacaagttcgatccgtgggacctcccatccaaggagaattacggggacagggagtggtacttcttcagcccgagggaccgcaagtacccgaacgggatccggccgaaccgcgccgcgggctccggctactggaaggccaccggcaccgacaagcccatccacagcagcggcggcgcggcgaccaacgagagcgtcggcgtcaagaaggcgctcgtcttctacaagggccgcccgcccaagggcaccaagaccaactggatcatgcacgagtaccgcctcgccgccgcagacgcccacgccgccaacacctaccgccccatgaagttccgcaacacctccatgagggtaagcaagctccatctacgttacctagcagccagattcaaattatctaaaccaaacaacttaattatgaacaaaccctag</cdnaseq>|
AA = <aaseq>MVLSNPAMLPPGFRFHPTDEELIVHYLRNRAASSPCPVSIIADV DIYKFDPWDLPSKENYGDREWYFFSPRDRKYPNGIRPNRAAGSGYWKATGTDKPIHSS GGAATNESVGVKKALVFYKGRPPKGTKTNWIMHEYRLAAADAHAANTYRPMKFRNTSM RVSKLHLRYLAARFKLSKPNNLIMNKP</aaseq>|
DNA = <dnaseqindica>440..837#180..345#aaattcgagccctcctccatctctcgcctcctcttgatacaagctcgaccctgatagatctcatagatcatagctgagaggggatcgagtggcttgatcttggaagtgagcagctatagctagataggttttgcttgtgcagggaggtgtgtgttgtgtgtgggtgactgccggagacgatggttctgtcgaacccggcgatgctgccgccggggttccggttccacccgacggacgaggagctgatcgtgcactacctccgcaaccgggccgcctcctcgccgtgccccgtctccatcatcgccgacgtcgacatctacaagttcgatccgtgggacctcccatgttagtacccaccctcacagctcgccgcgatcgatcgatcactgatttgcaattaatttcgttggtgatcgacttgtgttttgatttgcatcagccaaggagaattacggggacagggagtggtacttcttcagcccgagggaccgcaagtacccgaacgggatccggccgaaccgcgccgcgggctccggctactggaaggccaccggcaccgacaagcccatccacagcagcggcggcgcggcgaccaacgagagcgtcggcgtcaagaaggcgctcgtcttctacaagggccgcccgcccaagggcaccaagaccaactggatcatgcacgagtaccgcctcgccgccgcagacgcccacgccgccaacacctaccgccccatgaagttccgcaacacctccatgagggtaagcaagctccatctacgttacctagcagccagattcaaattatctaaaccaaacaacttaattatgaacaaaccctagcagctggatcttgctatcgatggagtagagagtagatcgtctattattcatctctgtctgatgtgctagtgcacttgcacagttgcacaccgtggtttttttgggtggttgtttctgatgatcgatacgtgttcgtcggcacgtcagtattatcgcatcggtttgtgccttgccaatggacgcaactcgctgcttttagtgtgtctgttttaaagagggtgttctttaggcaatacttggcatggttgcgcaagtcaatagatccgacaatgatagccacgatgcgtacgtgttgatgactggttcatctctgcatatgcgcgcatgcataccccctgaaattaatcccttctatttttgatactcttaatttcttaatcatatagtatatgcatccgtgtgattgatcgatcgatgtgcgtggatttttgtgcagctggatgactgggtgctgtgccggatctacaagaagagcagccacgcgtcgccgctggccgtgccgccgctctccgaccacgagcaggacgagccgtgcgccctggaggagaacgcgccgctgtacgcgccgtcgtcgtcgagcgccgcatccatgatcttgcagggcgctgccgccggcgccttcccgtcgctgcacgccgccgccgccgctacgcagaggacggcgatgcagaagatcccttccatttccgacctgctcaacgagtactcgctgtcgcagctcttcgacgacggcggcgccgccgccgccgcccccctgcaggagatggcgaggcagcccgaccaccaccaccaccaacaacaacaacacgccctctttggccaccccgtcatgaaccatttcatcgcgaacaacagcatggttcagctcgcgcacctggacccgtcctcctctgcggccgcctcgacgtcggcaggcgccgtcgtcgagccgccggccgtcaccgggaagcgcaagagatcatcggacggaggcgagccgacgatccaggcgctgcctcctgctgccgcggcggccaagaagccgaacggttcatgcgttggtgcaactttccaaataggcagcgccttgcagggatcgtcactgggactgagccaccagatgctgctccactctaacatggggatgaactgatagctcgttcttcgtacataataccacagcctgattaaacactgaacataattgcacatggcgaattgatgatttgaacagtgaggtctatttcgaaagcaaataggtccaaaacgatagatttaaatagtaaatatattgggaaagaaattgtacttaatttgtacttgtgtggactagctaggtcgtccgatcatgcatagctatatagtttgtggtgtgtatacattttttctcatggtactatactactgtgtactcaagctagctcagtactaaagattaattaagctagataggggttgataagactttgagatagacccactagaagtctggaacatgagatcaaggagaatgatgtcaagatcataaagccggatttatagctcatattcatgtaaatcaatcaataagtgattctttaatggcagccactcattttttt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001056532.2 RefSeq:Os03g0327800]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

File:Nap1.jpg

2014-07-01T12:00:28Z

Suchao13:

Os03g0327800

2014-07-01T11:59:59Z

Suchao13: /* Function */

Please input one-sentence summary here.

==Annotated Information==
===Function===

*Os03g0327800 encoding a Oryza sativa NAC-like, activated by apetala3/pistillata (OsNAP). Overexpression of OsNAP significantly promoted senescence, whereas knockdown of OsNAP produced a marked delay of senescence, confirming the role of this gene in the development of rice senescence.

*ABA Participates in Leaf Senescence and the expression of OsNAP can be induced by ABA. ABA-mediated leaf senescence might be dependent on the modulation of OsNAP expression, which, in turn, either directly or indirectly regulates the expression of senescence-associated genes (SAGs). Also, this is a feedback mechanism between ABA and OsNAP, OsNAP can inhibit the expression of key ABA biosynthesis genes.

[[File:nap1.jpg]]

===Expression===
Please input expression information here.

===Mutation and Phenotype===
Please input evolution information here.

===other information===

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0327800|
Description = No apical meristem (NAM) protein domain containing protein|
Version = NM_001056532.2 GI:297600855 GeneID:4332717|
Length = 2417 bp|
Definition = Oryza sativa Japonica Group Os03g0327800, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:12024636..12027052|
CDS = 12026216..12026613,12026708..12026873|
GCID = <gbrowseImage1>
name=NC_008396:12024636..12027052
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:12024636..12027052
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggttctgtcgaacccggcgatgctgccgccggggttccggttccacccgacggacgaggagctgatcgtgcactacctccgcaaccgggccgcctcctcgccgtgccccgtctccatcatcgccgacgtcgacatctacaagttcgatccgtgggacctcccatccaaggagaattacggggacagggagtggtacttcttcagcccgagggaccgcaagtacccgaacgggatccggccgaaccgcgccgcgggctccggctactggaaggccaccggcaccgacaagcccatccacagcagcggcggcgcggcgaccaacgagagcgtcggcgtcaagaaggcgctcgtcttctacaagggccgcccgcccaagggcaccaagaccaactggatcatgcacgagtaccgcctcgccgccgcagacgcccacgccgccaacacctaccgccccatgaagttccgcaacacctccatgagggtaagcaagctccatctacgttacctagcagccagattcaaattatctaaaccaaacaacttaattatgaacaaaccctag</cdnaseq>|
AA = <aaseq>MVLSNPAMLPPGFRFHPTDEELIVHYLRNRAASSPCPVSIIADV DIYKFDPWDLPSKENYGDREWYFFSPRDRKYPNGIRPNRAAGSGYWKATGTDKPIHSS GGAATNESVGVKKALVFYKGRPPKGTKTNWIMHEYRLAAADAHAANTYRPMKFRNTSM RVSKLHLRYLAARFKLSKPNNLIMNKP</aaseq>|
DNA = <dnaseqindica>440..837#180..345#aaattcgagccctcctccatctctcgcctcctcttgatacaagctcgaccctgatagatctcatagatcatagctgagaggggatcgagtggcttgatcttggaagtgagcagctatagctagataggttttgcttgtgcagggaggtgtgtgttgtgtgtgggtgactgccggagacgatggttctgtcgaacccggcgatgctgccgccggggttccggttccacccgacggacgaggagctgatcgtgcactacctccgcaaccgggccgcctcctcgccgtgccccgtctccatcatcgccgacgtcgacatctacaagttcgatccgtgggacctcccatgttagtacccaccctcacagctcgccgcgatcgatcgatcactgatttgcaattaatttcgttggtgatcgacttgtgttttgatttgcatcagccaaggagaattacggggacagggagtggtacttcttcagcccgagggaccgcaagtacccgaacgggatccggccgaaccgcgccgcgggctccggctactggaaggccaccggcaccgacaagcccatccacagcagcggcggcgcggcgaccaacgagagcgtcggcgtcaagaaggcgctcgtcttctacaagggccgcccgcccaagggcaccaagaccaactggatcatgcacgagtaccgcctcgccgccgcagacgcccacgccgccaacacctaccgccccatgaagttccgcaacacctccatgagggtaagcaagctccatctacgttacctagcagccagattcaaattatctaaaccaaacaacttaattatgaacaaaccctagcagctggatcttgctatcgatggagtagagagtagatcgtctattattcatctctgtctgatgtgctagtgcacttgcacagttgcacaccgtggtttttttgggtggttgtttctgatgatcgatacgtgttcgtcggcacgtcagtattatcgcatcggtttgtgccttgccaatggacgcaactcgctgcttttagtgtgtctgttttaaagagggtgttctttaggcaatacttggcatggttgcgcaagtcaatagatccgacaatgatagccacgatgcgtacgtgttgatgactggttcatctctgcatatgcgcgcatgcataccccctgaaattaatcccttctatttttgatactcttaatttcttaatcatatagtatatgcatccgtgtgattgatcgatcgatgtgcgtggatttttgtgcagctggatgactgggtgctgtgccggatctacaagaagagcagccacgcgtcgccgctggccgtgccgccgctctccgaccacgagcaggacgagccgtgcgccctggaggagaacgcgccgctgtacgcgccgtcgtcgtcgagcgccgcatccatgatcttgcagggcgctgccgccggcgccttcccgtcgctgcacgccgccgccgccgctacgcagaggacggcgatgcagaagatcccttccatttccgacctgctcaacgagtactcgctgtcgcagctcttcgacgacggcggcgccgccgccgccgcccccctgcaggagatggcgaggcagcccgaccaccaccaccaccaacaacaacaacacgccctctttggccaccccgtcatgaaccatttcatcgcgaacaacagcatggttcagctcgcgcacctggacccgtcctcctctgcggccgcctcgacgtcggcaggcgccgtcgtcgagccgccggccgtcaccgggaagcgcaagagatcatcggacggaggcgagccgacgatccaggcgctgcctcctgctgccgcggcggccaagaagccgaacggttcatgcgttggtgcaactttccaaataggcagcgccttgcagggatcgtcactgggactgagccaccagatgctgctccactctaacatggggatgaactgatagctcgttcttcgtacataataccacagcctgattaaacactgaacataattgcacatggcgaattgatgatttgaacagtgaggtctatttcgaaagcaaataggtccaaaacgatagatttaaatagtaaatatattgggaaagaaattgtacttaatttgtacttgtgtggactagctaggtcgtccgatcatgcatagctatatagtttgtggtgtgtatacattttttctcatggtactatactactgtgtactcaagctagctcagtactaaagattaattaagctagataggggttgataagactttgagatagacccactagaagtctggaacatgagatcaaggagaatgatgtcaagatcataaagccggatttatagctcatattcatgtaaatcaatcaataagtgattctttaatggcagccactcattttttt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001056532.2 RefSeq:Os03g0327800]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

Os03g0327800

2014-07-01T11:47:51Z

Suchao13: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.

===Expression===
Please input expression information here.

===Mutation and Phenotype===
Please input evolution information here.

===other information===

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os03g0327800|
Description = No apical meristem (NAM) protein domain containing protein|
Version = NM_001056532.2 GI:297600855 GeneID:4332717|
Length = 2417 bp|
Definition = Oryza sativa Japonica Group Os03g0327800, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
AP = Chromosome 3:12024636..12027052|
CDS = 12026216..12026613,12026708..12026873|
GCID = <gbrowseImage1>
name=NC_008396:12024636..12027052
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008396:12024636..12027052
source=RiceChromosome03
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggttctgtcgaacccggcgatgctgccgccggggttccggttccacccgacggacgaggagctgatcgtgcactacctccgcaaccgggccgcctcctcgccgtgccccgtctccatcatcgccgacgtcgacatctacaagttcgatccgtgggacctcccatccaaggagaattacggggacagggagtggtacttcttcagcccgagggaccgcaagtacccgaacgggatccggccgaaccgcgccgcgggctccggctactggaaggccaccggcaccgacaagcccatccacagcagcggcggcgcggcgaccaacgagagcgtcggcgtcaagaaggcgctcgtcttctacaagggccgcccgcccaagggcaccaagaccaactggatcatgcacgagtaccgcctcgccgccgcagacgcccacgccgccaacacctaccgccccatgaagttccgcaacacctccatgagggtaagcaagctccatctacgttacctagcagccagattcaaattatctaaaccaaacaacttaattatgaacaaaccctag</cdnaseq>|
AA = <aaseq>MVLSNPAMLPPGFRFHPTDEELIVHYLRNRAASSPCPVSIIADV DIYKFDPWDLPSKENYGDREWYFFSPRDRKYPNGIRPNRAAGSGYWKATGTDKPIHSS GGAATNESVGVKKALVFYKGRPPKGTKTNWIMHEYRLAAADAHAANTYRPMKFRNTSM RVSKLHLRYLAARFKLSKPNNLIMNKP</aaseq>|
DNA = <dnaseqindica>440..837#180..345#aaattcgagccctcctccatctctcgcctcctcttgatacaagctcgaccctgatagatctcatagatcatagctgagaggggatcgagtggcttgatcttggaagtgagcagctatagctagataggttttgcttgtgcagggaggtgtgtgttgtgtgtgggtgactgccggagacgatggttctgtcgaacccggcgatgctgccgccggggttccggttccacccgacggacgaggagctgatcgtgcactacctccgcaaccgggccgcctcctcgccgtgccccgtctccatcatcgccgacgtcgacatctacaagttcgatccgtgggacctcccatgttagtacccaccctcacagctcgccgcgatcgatcgatcactgatttgcaattaatttcgttggtgatcgacttgtgttttgatttgcatcagccaaggagaattacggggacagggagtggtacttcttcagcccgagggaccgcaagtacccgaacgggatccggccgaaccgcgccgcgggctccggctactggaaggccaccggcaccgacaagcccatccacagcagcggcggcgcggcgaccaacgagagcgtcggcgtcaagaaggcgctcgtcttctacaagggccgcccgcccaagggcaccaagaccaactggatcatgcacgagtaccgcctcgccgccgcagacgcccacgccgccaacacctaccgccccatgaagttccgcaacacctccatgagggtaagcaagctccatctacgttacctagcagccagattcaaattatctaaaccaaacaacttaattatgaacaaaccctagcagctggatcttgctatcgatggagtagagagtagatcgtctattattcatctctgtctgatgtgctagtgcacttgcacagttgcacaccgtggtttttttgggtggttgtttctgatgatcgatacgtgttcgtcggcacgtcagtattatcgcatcggtttgtgccttgccaatggacgcaactcgctgcttttagtgtgtctgttttaaagagggtgttctttaggcaatacttggcatggttgcgcaagtcaatagatccgacaatgatagccacgatgcgtacgtgttgatgactggttcatctctgcatatgcgcgcatgcataccccctgaaattaatcccttctatttttgatactcttaatttcttaatcatatagtatatgcatccgtgtgattgatcgatcgatgtgcgtggatttttgtgcagctggatgactgggtgctgtgccggatctacaagaagagcagccacgcgtcgccgctggccgtgccgccgctctccgaccacgagcaggacgagccgtgcgccctggaggagaacgcgccgctgtacgcgccgtcgtcgtcgagcgccgcatccatgatcttgcagggcgctgccgccggcgccttcccgtcgctgcacgccgccgccgccgctacgcagaggacggcgatgcagaagatcccttccatttccgacctgctcaacgagtactcgctgtcgcagctcttcgacgacggcggcgccgccgccgccgcccccctgcaggagatggcgaggcagcccgaccaccaccaccaccaacaacaacaacacgccctctttggccaccccgtcatgaaccatttcatcgcgaacaacagcatggttcagctcgcgcacctggacccgtcctcctctgcggccgcctcgacgtcggcaggcgccgtcgtcgagccgccggccgtcaccgggaagcgcaagagatcatcggacggaggcgagccgacgatccaggcgctgcctcctgctgccgcggcggccaagaagccgaacggttcatgcgttggtgcaactttccaaataggcagcgccttgcagggatcgtcactgggactgagccaccagatgctgctccactctaacatggggatgaactgatagctcgttcttcgtacataataccacagcctgattaaacactgaacataattgcacatggcgaattgatgatttgaacagtgaggtctatttcgaaagcaaataggtccaaaacgatagatttaaatagtaaatatattgggaaagaaattgtacttaatttgtacttgtgtggactagctaggtcgtccgatcatgcatagctatatagtttgtggtgtgtatacattttttctcatggtactatactactgtgtactcaagctagctcagtactaaagattaattaagctagataggggttgataagactttgagatagacccactagaagtctggaacatgagatcaaggagaatgatgtcaagatcataaagccggatttatagctcatattcatgtaaatcaatcaataagtgattctttaatggcagccactcattttttt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001056532.2 RefSeq:Os03g0327800]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 3]]
[[Category:Chromosome 3]]

Os01g0293100

2014-06-05T12:20:27Z

Suchao13: /* References */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.

TDR INTERACTING PROTEIN2 (TIP2) is a basic helix-loop-helix (bHLH) protein, functions as a crucial switch in the meristemoid transition and differentiation during early anther development.

* The tip2 mutants display undifferentiated inner three anther wall layers.

* The tip2 anthers have prolonged periclinal cell division activity after the formation of the inner two anther wall layers via periclinal cell division.

* The tip2 displayed undegenerated callose around meiocytes, suggesting defective callose degeneration in the tip2 mutant.

* The tip2 mutants display abort tapetal programmed cell death.

TIP2 acts upstream of TDR and EAT1 and directly regulates the expression of TDR and EAT1, suggested that the bHLH proteins TIP2, TDR, and EAT1 play
a central role in regulating differentiation, morphogenesis, and degradation of anther somatic cell layers.

[[File:tip1.jpg]]

===Mutation and Phenotype===
Please input information here.

TIP2 locus on chromosome 1 between Z6.1-5 and Z6.2-1, two markers that defined a 170-kb region.

[[File:tip4.jpg]]

tip2 is a deletion of two continuous G nucleotides in it second exon.

[[File:tip5.jpg]]

Compared with the wild type, tip2 has normal vegetative growth, inflorescence and flower morphology. However, tip2 has smaller anthers and does not produce
mature pollen grains during reproductive stage.

[[File:tip6.jpg]]

Compared with the wild type,the three inner somatic layers of tip2 anthers displayed no obvious difference in cell shape during stages 6 to 8.Furthermore, DAPI staining showed mutation in TIP2 does not directly affect meiotic initiation but causes the arrest of meiosis.
The rice tapetum marker gene,LIPID TRANSFER PROTEIN45 (LTP45)was preferentially expressed in tapetal cells at stage 7 and stage 8 in wild-type anthers, whereas in tip2 anthers its expression was not detectable.

[[File:tip7.jpg]]

In the wild type, meiocytes formed tetrads after meiosis, tapetal cells became condensed and initiated PCD-promoted degeneration, and the middle layer also started to be degraded.By contrast, cell morphology of the three inner anther wall layers of tip2 anthers remained similar, and no obvious tapetal PCD ignals were detected.

[[File:tip8.jpg]]

aniline blue showed tip2
displayed undegenerated callose around meiocytes.

[[File:tip9.jpg]]

===Expression===
Please input expression information here.

Using quantitative RT-PCR(qRT-PCR). TIP2 expression was specifically observed in anthers, from the meiosis stage to mitosis, with a maximum expression
level at stages 7 and 8.Interestingly, tip2 anthers displayed a lower level of TIP2 than the wild type from stage 6 to stage 8, but a higher level than the wild type after stage 9, when young microspores were released.

[[File:tip2.jpg]]

In situ hybridization showed strong expression of TIP2 in the middle layer and tapetum and weak expression in the endothecium in the wild type. By contrast, only background signal was detected in the same anther section with the sense probe.

[[File:tip3.jpg]]

===Evolution===
Please input evolution information here.

The TIP2 cDNA encodes a putative bHLH transcription factor containing a conserved bHLH domain and a Domain Unknown Function domain, which shares high sequence similarity with EAT1 and is homologous to Arabidopsis AtbHLH010, AtbHLH089, and AtbHLH091.

==Labs working on this gene==
Please input related labs here.

* State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University

* School of Life Science, Jiangsu Normal University

==References==
Please input cited references here.

* Zhenzhen Fu, Jing Yu, Xiaowei Cheng, et al. The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early
Anther Development.The Plant Cell, 2014

==Structured Information==
{{JaponicaGene|
GeneName = Os01g0293100|
Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|
Version = NM_001049330.1 GI:115436073 GeneID:4325164|
Length = 2112 bp|
Definition = Oryza sativa Japonica Group Os01g0293100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:10712748..10714859|
CDS = 10713003..10713074,10713260..10713712,10713812..10714426|
GCID = <gbrowseImage1>
name=NC_008394:10712748..10714859
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:10712748..10714859
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgtatcacccgcagtgcgagctcctgatgccgcttgagagcctggagatggacgtcggccagtcgcacctcgccgccgccgtcgcagcagccatgccgggggagctcaacttccacctcctccactcgctcgacgccgccgcggcggctgcctcctccaccgccgcctcggcctcctcccagcccaccgtcgactacttcttcggcggcgccgaccagcagccgccgccgccggcggcgatgcagtacgaccagctggcggcgccgcaccaccaccagacggtggccatgctgcgcgactactacggcggccactacccgccggcggcggcggcggcggcggccaccgaggcgtacttccgcggcgggccaaggacggccgggtcgtcgtcgctcgtgttcggcccggccgacgacgagtcggccttcatggtcggacccttcgagagctccccgacgccgcggtccggcggcggcaggaagcgtagccgcgccaccgccggcttccacggcggcgggccggccaacggcgtcgagaagaaggagaagcagcgccgcctgcggctcaccgagaagtacaacgccctcatgctcctcatccccaaccgcaccaaggaggatagagcgacggtgatctcagacgcgatcgagtacatccaggagctagggaggacggtggaggagctgacgctgctggtggagaagaagcggcggcggagggagatgcagggggacgtggtggacgcggcgacgtcgtcggtggtggcggggatggatcaggcggcggagagctcggagggcgaggtgatggcggcggcggcgatgggcgcggtggcaccgccgccgcggcaggcgccgatccggagcacgtacatccagcggcggagcaaggagacgttcgtggacgtgcggatcgtggaggacgacgtgaacatcaagctcaccaagcgccgccgcgacggctgtctcgccgccgcgtcgcgcgcgctggacgacctccgcctcgacctcgtccacctctccggcggcaagatcggcgactgccacatctacatgttcaacaccaagattcattcgggatctccagtgtttgcaagtgcagtggccagcaggctgattgaagtggtggatgagtactaa</cdnaseq>|
AA = <aaseq>MYHPQCELLMPLESLEMDVGQSHLAAAVAAAMPGELNFHLLHSL DAAAAAASSTAASASSQPTVDYFFGGADQQPPPPAAMQYDQLAAPHHHQTVAMLRDYY GGHYPPAAAAAAATEAYFRGGPRTAGSSSLVFGPADDESAFMVGPFESSPTPRSGGGR KRSRATAGFHGGGPANGVEKKEKQRRLRLTEKYNALMLLIPNRTKEDRATVISDAIEY IQELGRTVEELTLLVEKKRRRREMQGDVVDAATSSVVAGMDQAAESSEGEVMAAAAMG AVAPPPRQAPIRSTYIQRRSKETFVDVRIVEDDVNIKLTKRRRDGCLAAASRALDDLR LDLVHLSGGKIGDCHIYMFNTKIHSGSPVFASAVASRLIEVVDEY</aaseq>|
DNA = <dnaseqindica>1786..1857#1148..1600#434..1048#aagaaaccaactgctttctcctacccaatatcacccttgccccttttatatactcttcctctcatcaccttctcgatcggcctctctcctctcctctcatcagctcacacccccaaccaacaaacctagttaatttagctctagttggttcatccctgctgcactgcgagctcaagtaatcgatctgagctctgaagaaaaaggtctgtttcaaattgaaccctttcatctcatcttctcattactgtttgattcattaattctagtaatttagagtttaaaccatcgcttttctttttgtgtgtgttcttgcatatgcttggacgattttgagtttgttctttaaatttgggcttgaacataggttgattaagcgagaagagtttcatggtttggattcttgatttgttgcaggtggtagagtgcgaggaagatgtatcacccgcagtgcgagctcctgatgccgcttgagagcctggagatggacgtcggccagtcgcacctcgccgccgccgtcgcagcagccatgccgggggagctcaacttccacctcctccactcgctcgacgccgccgcggcggctgcctcctccaccgccgcctcggcctcctcccagcccaccgtcgactacttcttcggcggcgccgaccagcagccgccgccgccggcggcgatgcagtacgaccagctggcggcgccgcaccaccaccagacggtggccatgctgcgcgactactacggcggccactacccgccggcggcggcggcggcggcggccaccgaggcgtacttccgcggcgggccaaggacggccgggtcgtcgtcgctcgtgttcggcccggccgacgacgagtcggccttcatggtcggacccttcgagagctccccgacgccgcggtccggcggcggcaggaagcgtagccgcgccaccgccggcttccacggcggcgggccggccaacggcgtcgagaagaaggagaagcagcgccgcctgcggctcaccgagaagtacaacgccctcatgctcctcatccccaaccgcaccaaggtatatcacatgccctagctaccatcgtcttcggtagttcgatctcttcttgtgttcatccatggatcgatcgatgatgattttgtctttctttttcaggaggatagagcgacggtgatctcagacgcgatcgagtacatccaggagctagggaggacggtggaggagctgacgctgctggtggagaagaagcggcggcggagggagatgcagggggacgtggtggacgcggcgacgtcgtcggtggtggcggggatggatcaggcggcggagagctcggagggcgaggtgatggcggcggcggcgatgggcgcggtggcaccgccgccgcggcaggcgccgatccggagcacgtacatccagcggcggagcaaggagacgttcgtggacgtgcggatcgtggaggacgacgtgaacatcaagctcaccaagcgccgccgcgacggctgtctcgccgccgcgtcgcgcgcgctggacgacctccgcctcgacctcgtccacctctccggcggcaagatcggcgactgccacatctacatgttcaacaccaaggtcacaacaaccaactaaaaatatccccgcgtttatctacaaaactaaccaatctcataatagtaattaattctagaacaattttatcgtgttgtgatcaacataaacctatatatgcaaataaattgctgctagttaatctcatcattgatttattattgtttatttggatgcgatgcatgcagattcattcgggatctccagtgtttgcaagtgcagtggccagcaggctgattgaagtggtggatgagtactaactagctcgagctagctaattagccgaccgaccgatcgatatgatgaaagtttctatgttgctagctagctagggttcttggatgcatgagtactgagtagctctttaattaatttccttttaattttagactgtttaatttggattggtaaagactcgtgttagcttttgggagatctttggtatgtcatggtttgcatgtattattttggtctacttggataaataattgatgctctttgagacgttaattaat</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049330.1 RefSeq:Os01g0293100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

Os01g0293100

2014-06-05T12:19:18Z

Suchao13: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.

TDR INTERACTING PROTEIN2 (TIP2) is a basic helix-loop-helix (bHLH) protein, functions as a crucial switch in the meristemoid transition and differentiation during early anther development.

* The tip2 mutants display undifferentiated inner three anther wall layers.

* The tip2 anthers have prolonged periclinal cell division activity after the formation of the inner two anther wall layers via periclinal cell division.

* The tip2 displayed undegenerated callose around meiocytes, suggesting defective callose degeneration in the tip2 mutant.

* The tip2 mutants display abort tapetal programmed cell death.

TIP2 acts upstream of TDR and EAT1 and directly regulates the expression of TDR and EAT1, suggested that the bHLH proteins TIP2, TDR, and EAT1 play
a central role in regulating differentiation, morphogenesis, and degradation of anther somatic cell layers.

[[File:tip1.jpg]]

===Mutation and Phenotype===
Please input information here.

TIP2 locus on chromosome 1 between Z6.1-5 and Z6.2-1, two markers that defined a 170-kb region.

[[File:tip4.jpg]]

tip2 is a deletion of two continuous G nucleotides in it second exon.

[[File:tip5.jpg]]

Compared with the wild type, tip2 has normal vegetative growth, inflorescence and flower morphology. However, tip2 has smaller anthers and does not produce
mature pollen grains during reproductive stage.

[[File:tip6.jpg]]

Compared with the wild type,the three inner somatic layers of tip2 anthers displayed no obvious difference in cell shape during stages 6 to 8.Furthermore, DAPI staining showed mutation in TIP2 does not directly affect meiotic initiation but causes the arrest of meiosis.
The rice tapetum marker gene,LIPID TRANSFER PROTEIN45 (LTP45)was preferentially expressed in tapetal cells at stage 7 and stage 8 in wild-type anthers, whereas in tip2 anthers its expression was not detectable.

[[File:tip7.jpg]]

In the wild type, meiocytes formed tetrads after meiosis, tapetal cells became condensed and initiated PCD-promoted degeneration, and the middle layer also started to be degraded.By contrast, cell morphology of the three inner anther wall layers of tip2 anthers remained similar, and no obvious tapetal PCD ignals were detected.

[[File:tip8.jpg]]

aniline blue showed tip2
displayed undegenerated callose around meiocytes.

[[File:tip9.jpg]]

===Expression===
Please input expression information here.

Using quantitative RT-PCR(qRT-PCR). TIP2 expression was specifically observed in anthers, from the meiosis stage to mitosis, with a maximum expression
level at stages 7 and 8.Interestingly, tip2 anthers displayed a lower level of TIP2 than the wild type from stage 6 to stage 8, but a higher level than the wild type after stage 9, when young microspores were released.

[[File:tip2.jpg]]

In situ hybridization showed strong expression of TIP2 in the middle layer and tapetum and weak expression in the endothecium in the wild type. By contrast, only background signal was detected in the same anther section with the sense probe.

[[File:tip3.jpg]]

===Evolution===
Please input evolution information here.

The TIP2 cDNA encodes a putative bHLH transcription factor containing a conserved bHLH domain and a Domain Unknown Function domain, which shares high sequence similarity with EAT1 and is homologous to Arabidopsis AtbHLH010, AtbHLH089, and AtbHLH091.

==Labs working on this gene==
Please input related labs here.

* State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University

* School of Life Science, Jiangsu Normal University

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os01g0293100|
Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|
Version = NM_001049330.1 GI:115436073 GeneID:4325164|
Length = 2112 bp|
Definition = Oryza sativa Japonica Group Os01g0293100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:10712748..10714859|
CDS = 10713003..10713074,10713260..10713712,10713812..10714426|
GCID = <gbrowseImage1>
name=NC_008394:10712748..10714859
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:10712748..10714859
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgtatcacccgcagtgcgagctcctgatgccgcttgagagcctggagatggacgtcggccagtcgcacctcgccgccgccgtcgcagcagccatgccgggggagctcaacttccacctcctccactcgctcgacgccgccgcggcggctgcctcctccaccgccgcctcggcctcctcccagcccaccgtcgactacttcttcggcggcgccgaccagcagccgccgccgccggcggcgatgcagtacgaccagctggcggcgccgcaccaccaccagacggtggccatgctgcgcgactactacggcggccactacccgccggcggcggcggcggcggcggccaccgaggcgtacttccgcggcgggccaaggacggccgggtcgtcgtcgctcgtgttcggcccggccgacgacgagtcggccttcatggtcggacccttcgagagctccccgacgccgcggtccggcggcggcaggaagcgtagccgcgccaccgccggcttccacggcggcgggccggccaacggcgtcgagaagaaggagaagcagcgccgcctgcggctcaccgagaagtacaacgccctcatgctcctcatccccaaccgcaccaaggaggatagagcgacggtgatctcagacgcgatcgagtacatccaggagctagggaggacggtggaggagctgacgctgctggtggagaagaagcggcggcggagggagatgcagggggacgtggtggacgcggcgacgtcgtcggtggtggcggggatggatcaggcggcggagagctcggagggcgaggtgatggcggcggcggcgatgggcgcggtggcaccgccgccgcggcaggcgccgatccggagcacgtacatccagcggcggagcaaggagacgttcgtggacgtgcggatcgtggaggacgacgtgaacatcaagctcaccaagcgccgccgcgacggctgtctcgccgccgcgtcgcgcgcgctggacgacctccgcctcgacctcgtccacctctccggcggcaagatcggcgactgccacatctacatgttcaacaccaagattcattcgggatctccagtgtttgcaagtgcagtggccagcaggctgattgaagtggtggatgagtactaa</cdnaseq>|
AA = <aaseq>MYHPQCELLMPLESLEMDVGQSHLAAAVAAAMPGELNFHLLHSL DAAAAAASSTAASASSQPTVDYFFGGADQQPPPPAAMQYDQLAAPHHHQTVAMLRDYY GGHYPPAAAAAAATEAYFRGGPRTAGSSSLVFGPADDESAFMVGPFESSPTPRSGGGR KRSRATAGFHGGGPANGVEKKEKQRRLRLTEKYNALMLLIPNRTKEDRATVISDAIEY IQELGRTVEELTLLVEKKRRRREMQGDVVDAATSSVVAGMDQAAESSEGEVMAAAAMG AVAPPPRQAPIRSTYIQRRSKETFVDVRIVEDDVNIKLTKRRRDGCLAAASRALDDLR LDLVHLSGGKIGDCHIYMFNTKIHSGSPVFASAVASRLIEVVDEY</aaseq>|
DNA = <dnaseqindica>1786..1857#1148..1600#434..1048#aagaaaccaactgctttctcctacccaatatcacccttgccccttttatatactcttcctctcatcaccttctcgatcggcctctctcctctcctctcatcagctcacacccccaaccaacaaacctagttaatttagctctagttggttcatccctgctgcactgcgagctcaagtaatcgatctgagctctgaagaaaaaggtctgtttcaaattgaaccctttcatctcatcttctcattactgtttgattcattaattctagtaatttagagtttaaaccatcgcttttctttttgtgtgtgttcttgcatatgcttggacgattttgagtttgttctttaaatttgggcttgaacataggttgattaagcgagaagagtttcatggtttggattcttgatttgttgcaggtggtagagtgcgaggaagatgtatcacccgcagtgcgagctcctgatgccgcttgagagcctggagatggacgtcggccagtcgcacctcgccgccgccgtcgcagcagccatgccgggggagctcaacttccacctcctccactcgctcgacgccgccgcggcggctgcctcctccaccgccgcctcggcctcctcccagcccaccgtcgactacttcttcggcggcgccgaccagcagccgccgccgccggcggcgatgcagtacgaccagctggcggcgccgcaccaccaccagacggtggccatgctgcgcgactactacggcggccactacccgccggcggcggcggcggcggcggccaccgaggcgtacttccgcggcgggccaaggacggccgggtcgtcgtcgctcgtgttcggcccggccgacgacgagtcggccttcatggtcggacccttcgagagctccccgacgccgcggtccggcggcggcaggaagcgtagccgcgccaccgccggcttccacggcggcgggccggccaacggcgtcgagaagaaggagaagcagcgccgcctgcggctcaccgagaagtacaacgccctcatgctcctcatccccaaccgcaccaaggtatatcacatgccctagctaccatcgtcttcggtagttcgatctcttcttgtgttcatccatggatcgatcgatgatgattttgtctttctttttcaggaggatagagcgacggtgatctcagacgcgatcgagtacatccaggagctagggaggacggtggaggagctgacgctgctggtggagaagaagcggcggcggagggagatgcagggggacgtggtggacgcggcgacgtcgtcggtggtggcggggatggatcaggcggcggagagctcggagggcgaggtgatggcggcggcggcgatgggcgcggtggcaccgccgccgcggcaggcgccgatccggagcacgtacatccagcggcggagcaaggagacgttcgtggacgtgcggatcgtggaggacgacgtgaacatcaagctcaccaagcgccgccgcgacggctgtctcgccgccgcgtcgcgcgcgctggacgacctccgcctcgacctcgtccacctctccggcggcaagatcggcgactgccacatctacatgttcaacaccaaggtcacaacaaccaactaaaaatatccccgcgtttatctacaaaactaaccaatctcataatagtaattaattctagaacaattttatcgtgttgtgatcaacataaacctatatatgcaaataaattgctgctagttaatctcatcattgatttattattgtttatttggatgcgatgcatgcagattcattcgggatctccagtgtttgcaagtgcagtggccagcaggctgattgaagtggtggatgagtactaactagctcgagctagctaattagccgaccgaccgatcgatatgatgaaagtttctatgttgctagctagctagggttcttggatgcatgagtactgagtagctctttaattaatttccttttaattttagactgtttaatttggattggtaaagactcgtgttagcttttgggagatctttggtatgtcatggtttgcatgtattattttggtctacttggataaataattgatgctctttgagacgttaattaat</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049330.1 RefSeq:Os01g0293100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

Os01g0293100

2014-06-05T12:18:34Z

Suchao13: /* Evolution */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.

TDR INTERACTING PROTEIN2 (TIP2) is a basic helix-loop-helix (bHLH) protein, functions as a crucial switch in the meristemoid transition and differentiation during early anther development.

* The tip2 mutants display undifferentiated inner three anther wall layers.

* The tip2 anthers have prolonged periclinal cell division activity after the formation of the inner two anther wall layers via periclinal cell division.

* The tip2 displayed undegenerated callose around meiocytes, suggesting defective callose degeneration in the tip2 mutant.

* The tip2 mutants display abort tapetal programmed cell death.

TIP2 acts upstream of TDR and EAT1 and directly regulates the expression of TDR and EAT1, suggested that the bHLH proteins TIP2, TDR, and EAT1 play
a central role in regulating differentiation, morphogenesis, and degradation of anther somatic cell layers.

[[File:tip1.jpg]]

===Mutation and Phenotype===
Please input information here.

TIP2 locus on chromosome 1 between Z6.1-5 and Z6.2-1, two markers that defined a 170-kb region.

[[File:tip4.jpg]]

tip2 is a deletion of two continuous G nucleotides in it second exon.

[[File:tip5.jpg]]

Compared with the wild type, tip2 has normal vegetative growth, inflorescence and flower morphology. However, tip2 has smaller anthers and does not produce
mature pollen grains during reproductive stage.

[[File:tip6.jpg]]

Compared with the wild type,the three inner somatic layers of tip2 anthers displayed no obvious difference in cell shape during stages 6 to 8.Furthermore, DAPI staining showed mutation in TIP2 does not directly affect meiotic initiation but causes the arrest of meiosis.
The rice tapetum marker gene,LIPID TRANSFER PROTEIN45 (LTP45)was preferentially expressed in tapetal cells at stage 7 and stage 8 in wild-type anthers, whereas in tip2 anthers its expression was not detectable.

[[File:tip7.jpg]]

In the wild type, meiocytes formed tetrads after meiosis, tapetal cells became condensed and initiated PCD-promoted degeneration, and the middle layer also started to be degraded.By contrast, cell morphology of the three inner anther wall layers of tip2 anthers remained similar, and no obvious tapetal PCD ignals were detected.

[[File:tip8.jpg]]

aniline blue showed tip2
displayed undegenerated callose around meiocytes.

[[File:tip9.jpg]]

===Expression===
Please input expression information here.

Using quantitative RT-PCR(qRT-PCR). TIP2 expression was specifically observed in anthers, from the meiosis stage to mitosis, with a maximum expression
level at stages 7 and 8.Interestingly, tip2 anthers displayed a lower level of TIP2 than the wild type from stage 6 to stage 8, but a higher level than the wild type after stage 9, when young microspores were released.

[[File:tip2.jpg]]

In situ hybridization showed strong expression of TIP2 in the middle layer and tapetum and weak expression in the endothecium in the wild type. By contrast, only background signal was detected in the same anther section with the sense probe.

[[File:tip3.jpg]]

===Evolution===
Please input evolution information here.

The TIP2 cDNA encodes a putative bHLH transcription factor containing a conserved bHLH domain and a Domain Unknown Function domain, which shares high sequence similarity with EAT1 and is homologous to Arabidopsis AtbHLH010, AtbHLH089, and AtbHLH091.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os01g0293100|
Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|
Version = NM_001049330.1 GI:115436073 GeneID:4325164|
Length = 2112 bp|
Definition = Oryza sativa Japonica Group Os01g0293100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:10712748..10714859|
CDS = 10713003..10713074,10713260..10713712,10713812..10714426|
GCID = <gbrowseImage1>
name=NC_008394:10712748..10714859
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:10712748..10714859
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgtatcacccgcagtgcgagctcctgatgccgcttgagagcctggagatggacgtcggccagtcgcacctcgccgccgccgtcgcagcagccatgccgggggagctcaacttccacctcctccactcgctcgacgccgccgcggcggctgcctcctccaccgccgcctcggcctcctcccagcccaccgtcgactacttcttcggcggcgccgaccagcagccgccgccgccggcggcgatgcagtacgaccagctggcggcgccgcaccaccaccagacggtggccatgctgcgcgactactacggcggccactacccgccggcggcggcggcggcggcggccaccgaggcgtacttccgcggcgggccaaggacggccgggtcgtcgtcgctcgtgttcggcccggccgacgacgagtcggccttcatggtcggacccttcgagagctccccgacgccgcggtccggcggcggcaggaagcgtagccgcgccaccgccggcttccacggcggcgggccggccaacggcgtcgagaagaaggagaagcagcgccgcctgcggctcaccgagaagtacaacgccctcatgctcctcatccccaaccgcaccaaggaggatagagcgacggtgatctcagacgcgatcgagtacatccaggagctagggaggacggtggaggagctgacgctgctggtggagaagaagcggcggcggagggagatgcagggggacgtggtggacgcggcgacgtcgtcggtggtggcggggatggatcaggcggcggagagctcggagggcgaggtgatggcggcggcggcgatgggcgcggtggcaccgccgccgcggcaggcgccgatccggagcacgtacatccagcggcggagcaaggagacgttcgtggacgtgcggatcgtggaggacgacgtgaacatcaagctcaccaagcgccgccgcgacggctgtctcgccgccgcgtcgcgcgcgctggacgacctccgcctcgacctcgtccacctctccggcggcaagatcggcgactgccacatctacatgttcaacaccaagattcattcgggatctccagtgtttgcaagtgcagtggccagcaggctgattgaagtggtggatgagtactaa</cdnaseq>|
AA = <aaseq>MYHPQCELLMPLESLEMDVGQSHLAAAVAAAMPGELNFHLLHSL DAAAAAASSTAASASSQPTVDYFFGGADQQPPPPAAMQYDQLAAPHHHQTVAMLRDYY GGHYPPAAAAAAATEAYFRGGPRTAGSSSLVFGPADDESAFMVGPFESSPTPRSGGGR KRSRATAGFHGGGPANGVEKKEKQRRLRLTEKYNALMLLIPNRTKEDRATVISDAIEY IQELGRTVEELTLLVEKKRRRREMQGDVVDAATSSVVAGMDQAAESSEGEVMAAAAMG AVAPPPRQAPIRSTYIQRRSKETFVDVRIVEDDVNIKLTKRRRDGCLAAASRALDDLR LDLVHLSGGKIGDCHIYMFNTKIHSGSPVFASAVASRLIEVVDEY</aaseq>|
DNA = <dnaseqindica>1786..1857#1148..1600#434..1048#aagaaaccaactgctttctcctacccaatatcacccttgccccttttatatactcttcctctcatcaccttctcgatcggcctctctcctctcctctcatcagctcacacccccaaccaacaaacctagttaatttagctctagttggttcatccctgctgcactgcgagctcaagtaatcgatctgagctctgaagaaaaaggtctgtttcaaattgaaccctttcatctcatcttctcattactgtttgattcattaattctagtaatttagagtttaaaccatcgcttttctttttgtgtgtgttcttgcatatgcttggacgattttgagtttgttctttaaatttgggcttgaacataggttgattaagcgagaagagtttcatggtttggattcttgatttgttgcaggtggtagagtgcgaggaagatgtatcacccgcagtgcgagctcctgatgccgcttgagagcctggagatggacgtcggccagtcgcacctcgccgccgccgtcgcagcagccatgccgggggagctcaacttccacctcctccactcgctcgacgccgccgcggcggctgcctcctccaccgccgcctcggcctcctcccagcccaccgtcgactacttcttcggcggcgccgaccagcagccgccgccgccggcggcgatgcagtacgaccagctggcggcgccgcaccaccaccagacggtggccatgctgcgcgactactacggcggccactacccgccggcggcggcggcggcggcggccaccgaggcgtacttccgcggcgggccaaggacggccgggtcgtcgtcgctcgtgttcggcccggccgacgacgagtcggccttcatggtcggacccttcgagagctccccgacgccgcggtccggcggcggcaggaagcgtagccgcgccaccgccggcttccacggcggcgggccggccaacggcgtcgagaagaaggagaagcagcgccgcctgcggctcaccgagaagtacaacgccctcatgctcctcatccccaaccgcaccaaggtatatcacatgccctagctaccatcgtcttcggtagttcgatctcttcttgtgttcatccatggatcgatcgatgatgattttgtctttctttttcaggaggatagagcgacggtgatctcagacgcgatcgagtacatccaggagctagggaggacggtggaggagctgacgctgctggtggagaagaagcggcggcggagggagatgcagggggacgtggtggacgcggcgacgtcgtcggtggtggcggggatggatcaggcggcggagagctcggagggcgaggtgatggcggcggcggcgatgggcgcggtggcaccgccgccgcggcaggcgccgatccggagcacgtacatccagcggcggagcaaggagacgttcgtggacgtgcggatcgtggaggacgacgtgaacatcaagctcaccaagcgccgccgcgacggctgtctcgccgccgcgtcgcgcgcgctggacgacctccgcctcgacctcgtccacctctccggcggcaagatcggcgactgccacatctacatgttcaacaccaaggtcacaacaaccaactaaaaatatccccgcgtttatctacaaaactaaccaatctcataatagtaattaattctagaacaattttatcgtgttgtgatcaacataaacctatatatgcaaataaattgctgctagttaatctcatcattgatttattattgtttatttggatgcgatgcatgcagattcattcgggatctccagtgtttgcaagtgcagtggccagcaggctgattgaagtggtggatgagtactaactagctcgagctagctaattagccgaccgaccgatcgatatgatgaaagtttctatgttgctagctagctagggttcttggatgcatgagtactgagtagctctttaattaatttccttttaattttagactgtttaatttggattggtaaagactcgtgttagcttttgggagatctttggtatgtcatggtttgcatgtattattttggtctacttggataaataattgatgctctttgagacgttaattaat</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049330.1 RefSeq:Os01g0293100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

File:Tip9.jpg

2014-06-05T12:16:42Z

Suchao13:

File:Tip8.jpg

2014-06-05T12:16:01Z

Suchao13:

File:Tip7.jpg

2014-06-05T12:15:10Z

Suchao13:

File:Tip6.jpg

2014-06-05T12:13:25Z

Suchao13:

File:Tip5.jpg

2014-06-05T12:12:36Z

Suchao13:

File:Tip4.jpg

2014-06-05T12:12:15Z

Suchao13:

Os01g0293100

2014-06-05T12:11:13Z

Suchao13: /* Mutation and Phenotype */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.

TDR INTERACTING PROTEIN2 (TIP2) is a basic helix-loop-helix (bHLH) protein, functions as a crucial switch in the meristemoid transition and differentiation during early anther development.

* The tip2 mutants display undifferentiated inner three anther wall layers.

* The tip2 anthers have prolonged periclinal cell division activity after the formation of the inner two anther wall layers via periclinal cell division.

* The tip2 displayed undegenerated callose around meiocytes, suggesting defective callose degeneration in the tip2 mutant.

* The tip2 mutants display abort tapetal programmed cell death.

TIP2 acts upstream of TDR and EAT1 and directly regulates the expression of TDR and EAT1, suggested that the bHLH proteins TIP2, TDR, and EAT1 play
a central role in regulating differentiation, morphogenesis, and degradation of anther somatic cell layers.

[[File:tip1.jpg]]

===Mutation and Phenotype===
Please input information here.

TIP2 locus on chromosome 1 between Z6.1-5 and Z6.2-1, two markers that defined a 170-kb region.

[[File:tip4.jpg]]

tip2 is a deletion of two continuous G nucleotides in it second exon.

[[File:tip5.jpg]]

Compared with the wild type, tip2 has normal vegetative growth, inflorescence and flower morphology. However, tip2 has smaller anthers and does not produce
mature pollen grains during reproductive stage.

[[File:tip6.jpg]]

Compared with the wild type,the three inner somatic layers of tip2 anthers displayed no obvious difference in cell shape during stages 6 to 8.Furthermore, DAPI staining showed mutation in TIP2 does not directly affect meiotic initiation but causes the arrest of meiosis.
The rice tapetum marker gene,LIPID TRANSFER PROTEIN45 (LTP45)was preferentially expressed in tapetal cells at stage 7 and stage 8 in wild-type anthers, whereas in tip2 anthers its expression was not detectable.

[[File:tip7.jpg]]

In the wild type, meiocytes formed tetrads after meiosis, tapetal cells became condensed and initiated PCD-promoted degeneration, and the middle layer also started to be degraded.By contrast, cell morphology of the three inner anther wall layers of tip2 anthers remained similar, and no obvious tapetal PCD ignals were detected.

[[File:tip8.jpg]]

aniline blue showed tip2
displayed undegenerated callose around meiocytes.

[[File:tip9.jpg]]

===Expression===
Please input expression information here.

Using quantitative RT-PCR(qRT-PCR). TIP2 expression was specifically observed in anthers, from the meiosis stage to mitosis, with a maximum expression
level at stages 7 and 8.Interestingly, tip2 anthers displayed a lower level of TIP2 than the wild type from stage 6 to stage 8, but a higher level than the wild type after stage 9, when young microspores were released.

[[File:tip2.jpg]]

In situ hybridization showed strong expression of TIP2 in the middle layer and tapetum and weak expression in the endothecium in the wild type. By contrast, only background signal was detected in the same anther section with the sense probe.

[[File:tip3.jpg]]

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os01g0293100|
Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|
Version = NM_001049330.1 GI:115436073 GeneID:4325164|
Length = 2112 bp|
Definition = Oryza sativa Japonica Group Os01g0293100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:10712748..10714859|
CDS = 10713003..10713074,10713260..10713712,10713812..10714426|
GCID = <gbrowseImage1>
name=NC_008394:10712748..10714859
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:10712748..10714859
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgtatcacccgcagtgcgagctcctgatgccgcttgagagcctggagatggacgtcggccagtcgcacctcgccgccgccgtcgcagcagccatgccgggggagctcaacttccacctcctccactcgctcgacgccgccgcggcggctgcctcctccaccgccgcctcggcctcctcccagcccaccgtcgactacttcttcggcggcgccgaccagcagccgccgccgccggcggcgatgcagtacgaccagctggcggcgccgcaccaccaccagacggtggccatgctgcgcgactactacggcggccactacccgccggcggcggcggcggcggcggccaccgaggcgtacttccgcggcgggccaaggacggccgggtcgtcgtcgctcgtgttcggcccggccgacgacgagtcggccttcatggtcggacccttcgagagctccccgacgccgcggtccggcggcggcaggaagcgtagccgcgccaccgccggcttccacggcggcgggccggccaacggcgtcgagaagaaggagaagcagcgccgcctgcggctcaccgagaagtacaacgccctcatgctcctcatccccaaccgcaccaaggaggatagagcgacggtgatctcagacgcgatcgagtacatccaggagctagggaggacggtggaggagctgacgctgctggtggagaagaagcggcggcggagggagatgcagggggacgtggtggacgcggcgacgtcgtcggtggtggcggggatggatcaggcggcggagagctcggagggcgaggtgatggcggcggcggcgatgggcgcggtggcaccgccgccgcggcaggcgccgatccggagcacgtacatccagcggcggagcaaggagacgttcgtggacgtgcggatcgtggaggacgacgtgaacatcaagctcaccaagcgccgccgcgacggctgtctcgccgccgcgtcgcgcgcgctggacgacctccgcctcgacctcgtccacctctccggcggcaagatcggcgactgccacatctacatgttcaacaccaagattcattcgggatctccagtgtttgcaagtgcagtggccagcaggctgattgaagtggtggatgagtactaa</cdnaseq>|
AA = <aaseq>MYHPQCELLMPLESLEMDVGQSHLAAAVAAAMPGELNFHLLHSL DAAAAAASSTAASASSQPTVDYFFGGADQQPPPPAAMQYDQLAAPHHHQTVAMLRDYY GGHYPPAAAAAAATEAYFRGGPRTAGSSSLVFGPADDESAFMVGPFESSPTPRSGGGR KRSRATAGFHGGGPANGVEKKEKQRRLRLTEKYNALMLLIPNRTKEDRATVISDAIEY IQELGRTVEELTLLVEKKRRRREMQGDVVDAATSSVVAGMDQAAESSEGEVMAAAAMG AVAPPPRQAPIRSTYIQRRSKETFVDVRIVEDDVNIKLTKRRRDGCLAAASRALDDLR LDLVHLSGGKIGDCHIYMFNTKIHSGSPVFASAVASRLIEVVDEY</aaseq>|
DNA = <dnaseqindica>1786..1857#1148..1600#434..1048#aagaaaccaactgctttctcctacccaatatcacccttgccccttttatatactcttcctctcatcaccttctcgatcggcctctctcctctcctctcatcagctcacacccccaaccaacaaacctagttaatttagctctagttggttcatccctgctgcactgcgagctcaagtaatcgatctgagctctgaagaaaaaggtctgtttcaaattgaaccctttcatctcatcttctcattactgtttgattcattaattctagtaatttagagtttaaaccatcgcttttctttttgtgtgtgttcttgcatatgcttggacgattttgagtttgttctttaaatttgggcttgaacataggttgattaagcgagaagagtttcatggtttggattcttgatttgttgcaggtggtagagtgcgaggaagatgtatcacccgcagtgcgagctcctgatgccgcttgagagcctggagatggacgtcggccagtcgcacctcgccgccgccgtcgcagcagccatgccgggggagctcaacttccacctcctccactcgctcgacgccgccgcggcggctgcctcctccaccgccgcctcggcctcctcccagcccaccgtcgactacttcttcggcggcgccgaccagcagccgccgccgccggcggcgatgcagtacgaccagctggcggcgccgcaccaccaccagacggtggccatgctgcgcgactactacggcggccactacccgccggcggcggcggcggcggcggccaccgaggcgtacttccgcggcgggccaaggacggccgggtcgtcgtcgctcgtgttcggcccggccgacgacgagtcggccttcatggtcggacccttcgagagctccccgacgccgcggtccggcggcggcaggaagcgtagccgcgccaccgccggcttccacggcggcgggccggccaacggcgtcgagaagaaggagaagcagcgccgcctgcggctcaccgagaagtacaacgccctcatgctcctcatccccaaccgcaccaaggtatatcacatgccctagctaccatcgtcttcggtagttcgatctcttcttgtgttcatccatggatcgatcgatgatgattttgtctttctttttcaggaggatagagcgacggtgatctcagacgcgatcgagtacatccaggagctagggaggacggtggaggagctgacgctgctggtggagaagaagcggcggcggagggagatgcagggggacgtggtggacgcggcgacgtcgtcggtggtggcggggatggatcaggcggcggagagctcggagggcgaggtgatggcggcggcggcgatgggcgcggtggcaccgccgccgcggcaggcgccgatccggagcacgtacatccagcggcggagcaaggagacgttcgtggacgtgcggatcgtggaggacgacgtgaacatcaagctcaccaagcgccgccgcgacggctgtctcgccgccgcgtcgcgcgcgctggacgacctccgcctcgacctcgtccacctctccggcggcaagatcggcgactgccacatctacatgttcaacaccaaggtcacaacaaccaactaaaaatatccccgcgtttatctacaaaactaaccaatctcataatagtaattaattctagaacaattttatcgtgttgtgatcaacataaacctatatatgcaaataaattgctgctagttaatctcatcattgatttattattgtttatttggatgcgatgcatgcagattcattcgggatctccagtgtttgcaagtgcagtggccagcaggctgattgaagtggtggatgagtactaactagctcgagctagctaattagccgaccgaccgatcgatatgatgaaagtttctatgttgctagctagctagggttcttggatgcatgagtactgagtagctctttaattaatttccttttaattttagactgtttaatttggattggtaaagactcgtgttagcttttgggagatctttggtatgtcatggtttgcatgtattattttggtctacttggataaataattgatgctctttgagacgttaattaat</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049330.1 RefSeq:Os01g0293100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

Os01g0293100

2014-06-05T12:00:46Z

Suchao13: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.

TDR INTERACTING PROTEIN2 (TIP2) is a basic helix-loop-helix (bHLH) protein, functions as a crucial switch in the meristemoid transition and differentiation during early anther development.

* The tip2 mutants display undifferentiated inner three anther wall layers.

* The tip2 anthers have prolonged periclinal cell division activity after the formation of the inner two anther wall layers via periclinal cell division.

* The tip2 displayed undegenerated callose around meiocytes, suggesting defective callose degeneration in the tip2 mutant.

* The tip2 mutants display abort tapetal programmed cell death.

TIP2 acts upstream of TDR and EAT1 and directly regulates the expression of TDR and EAT1, suggested that the bHLH proteins TIP2, TDR, and EAT1 play
a central role in regulating differentiation, morphogenesis, and degradation of anther somatic cell layers.

[[File:tip1.jpg]]

===Mutation and Phenotype===
Please input information here.

===Expression===
Please input expression information here.

Using quantitative RT-PCR(qRT-PCR). TIP2 expression was specifically observed in anthers, from the meiosis stage to mitosis, with a maximum expression
level at stages 7 and 8.Interestingly, tip2 anthers displayed a lower level of TIP2 than the wild type from stage 6 to stage 8, but a higher level than the wild type after stage 9, when young microspores were released.

[[File:tip2.jpg]]

In situ hybridization showed strong expression of TIP2 in the middle layer and tapetum and weak expression in the endothecium in the wild type. By contrast, only background signal was detected in the same anther section with the sense probe.

[[File:tip3.jpg]]

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os01g0293100|
Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|
Version = NM_001049330.1 GI:115436073 GeneID:4325164|
Length = 2112 bp|
Definition = Oryza sativa Japonica Group Os01g0293100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:10712748..10714859|
CDS = 10713003..10713074,10713260..10713712,10713812..10714426|
GCID = <gbrowseImage1>
name=NC_008394:10712748..10714859
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:10712748..10714859
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgtatcacccgcagtgcgagctcctgatgccgcttgagagcctggagatggacgtcggccagtcgcacctcgccgccgccgtcgcagcagccatgccgggggagctcaacttccacctcctccactcgctcgacgccgccgcggcggctgcctcctccaccgccgcctcggcctcctcccagcccaccgtcgactacttcttcggcggcgccgaccagcagccgccgccgccggcggcgatgcagtacgaccagctggcggcgccgcaccaccaccagacggtggccatgctgcgcgactactacggcggccactacccgccggcggcggcggcggcggcggccaccgaggcgtacttccgcggcgggccaaggacggccgggtcgtcgtcgctcgtgttcggcccggccgacgacgagtcggccttcatggtcggacccttcgagagctccccgacgccgcggtccggcggcggcaggaagcgtagccgcgccaccgccggcttccacggcggcgggccggccaacggcgtcgagaagaaggagaagcagcgccgcctgcggctcaccgagaagtacaacgccctcatgctcctcatccccaaccgcaccaaggaggatagagcgacggtgatctcagacgcgatcgagtacatccaggagctagggaggacggtggaggagctgacgctgctggtggagaagaagcggcggcggagggagatgcagggggacgtggtggacgcggcgacgtcgtcggtggtggcggggatggatcaggcggcggagagctcggagggcgaggtgatggcggcggcggcgatgggcgcggtggcaccgccgccgcggcaggcgccgatccggagcacgtacatccagcggcggagcaaggagacgttcgtggacgtgcggatcgtggaggacgacgtgaacatcaagctcaccaagcgccgccgcgacggctgtctcgccgccgcgtcgcgcgcgctggacgacctccgcctcgacctcgtccacctctccggcggcaagatcggcgactgccacatctacatgttcaacaccaagattcattcgggatctccagtgtttgcaagtgcagtggccagcaggctgattgaagtggtggatgagtactaa</cdnaseq>|
AA = <aaseq>MYHPQCELLMPLESLEMDVGQSHLAAAVAAAMPGELNFHLLHSL DAAAAAASSTAASASSQPTVDYFFGGADQQPPPPAAMQYDQLAAPHHHQTVAMLRDYY GGHYPPAAAAAAATEAYFRGGPRTAGSSSLVFGPADDESAFMVGPFESSPTPRSGGGR KRSRATAGFHGGGPANGVEKKEKQRRLRLTEKYNALMLLIPNRTKEDRATVISDAIEY IQELGRTVEELTLLVEKKRRRREMQGDVVDAATSSVVAGMDQAAESSEGEVMAAAAMG AVAPPPRQAPIRSTYIQRRSKETFVDVRIVEDDVNIKLTKRRRDGCLAAASRALDDLR LDLVHLSGGKIGDCHIYMFNTKIHSGSPVFASAVASRLIEVVDEY</aaseq>|
DNA = <dnaseqindica>1786..1857#1148..1600#434..1048#aagaaaccaactgctttctcctacccaatatcacccttgccccttttatatactcttcctctcatcaccttctcgatcggcctctctcctctcctctcatcagctcacacccccaaccaacaaacctagttaatttagctctagttggttcatccctgctgcactgcgagctcaagtaatcgatctgagctctgaagaaaaaggtctgtttcaaattgaaccctttcatctcatcttctcattactgtttgattcattaattctagtaatttagagtttaaaccatcgcttttctttttgtgtgtgttcttgcatatgcttggacgattttgagtttgttctttaaatttgggcttgaacataggttgattaagcgagaagagtttcatggtttggattcttgatttgttgcaggtggtagagtgcgaggaagatgtatcacccgcagtgcgagctcctgatgccgcttgagagcctggagatggacgtcggccagtcgcacctcgccgccgccgtcgcagcagccatgccgggggagctcaacttccacctcctccactcgctcgacgccgccgcggcggctgcctcctccaccgccgcctcggcctcctcccagcccaccgtcgactacttcttcggcggcgccgaccagcagccgccgccgccggcggcgatgcagtacgaccagctggcggcgccgcaccaccaccagacggtggccatgctgcgcgactactacggcggccactacccgccggcggcggcggcggcggcggccaccgaggcgtacttccgcggcgggccaaggacggccgggtcgtcgtcgctcgtgttcggcccggccgacgacgagtcggccttcatggtcggacccttcgagagctccccgacgccgcggtccggcggcggcaggaagcgtagccgcgccaccgccggcttccacggcggcgggccggccaacggcgtcgagaagaaggagaagcagcgccgcctgcggctcaccgagaagtacaacgccctcatgctcctcatccccaaccgcaccaaggtatatcacatgccctagctaccatcgtcttcggtagttcgatctcttcttgtgttcatccatggatcgatcgatgatgattttgtctttctttttcaggaggatagagcgacggtgatctcagacgcgatcgagtacatccaggagctagggaggacggtggaggagctgacgctgctggtggagaagaagcggcggcggagggagatgcagggggacgtggtggacgcggcgacgtcgtcggtggtggcggggatggatcaggcggcggagagctcggagggcgaggtgatggcggcggcggcgatgggcgcggtggcaccgccgccgcggcaggcgccgatccggagcacgtacatccagcggcggagcaaggagacgttcgtggacgtgcggatcgtggaggacgacgtgaacatcaagctcaccaagcgccgccgcgacggctgtctcgccgccgcgtcgcgcgcgctggacgacctccgcctcgacctcgtccacctctccggcggcaagatcggcgactgccacatctacatgttcaacaccaaggtcacaacaaccaactaaaaatatccccgcgtttatctacaaaactaaccaatctcataatagtaattaattctagaacaattttatcgtgttgtgatcaacataaacctatatatgcaaataaattgctgctagttaatctcatcattgatttattattgtttatttggatgcgatgcatgcagattcattcgggatctccagtgtttgcaagtgcagtggccagcaggctgattgaagtggtggatgagtactaactagctcgagctagctaattagccgaccgaccgatcgatatgatgaaagtttctatgttgctagctagctagggttcttggatgcatgagtactgagtagctctttaattaatttccttttaattttagactgtttaatttggattggtaaagactcgtgttagcttttgggagatctttggtatgtcatggtttgcatgtattattttggtctacttggataaataattgatgctctttgagacgttaattaat</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049330.1 RefSeq:Os01g0293100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

File:Tip3.jpg

2014-06-05T11:59:13Z

Suchao13:

File:Tip2.jpg

2014-06-05T11:58:44Z

Suchao13:

Os01g0293100

2014-06-05T11:57:45Z

Suchao13: /* Expression */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.

TDR INTERACTING PROTEIN2 (TIP2) is a basic helix-loop-helix (bHLH) protein, functions as a crucial switch in the meristemoid transition and differentiation during early anther development.

* The tip2 mutants display undifferentiated inner three anther wall layers.

* The tip2 anthers have prolonged periclinal cell division activity after the formation of the inner two anther wall layers via periclinal cell division.

* The tip2 displayed undegenerated callose around meiocytes, suggesting defective callose degeneration in the tip2 mutant.

* The tip2 mutants display abort tapetal programmed cell death.

TIP2 acts upstream of TDR and EAT1 and directly regulates the expression of TDR and EAT1, suggested that the bHLH proteins TIP2, TDR, and EAT1 play
a central role in regulating differentiation, morphogenesis, and degradation of anther somatic cell layers.

[[File:tip1.jpg]]

===Expression===
Please input expression information here.

Using quantitative RT-PCR(qRT-PCR). TIP2 expression was specifically observed in anthers, from the meiosis stage to mitosis, with a maximum expression
level at stages 7 and 8.Interestingly, tip2 anthers displayed a lower level of TIP2 than the wild type from stage 6 to stage 8, but a higher level than the wild type after stage 9, when young microspores were released.

[[File:tip2.jpg]]

In situ hybridization showed strong expression of TIP2 in the middle layer and tapetum and weak expression in the endothecium in the wild type. By contrast, only background signal was detected in the same anther section with the sense probe.

[[File:tip3.jpg]]

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os01g0293100|
Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|
Version = NM_001049330.1 GI:115436073 GeneID:4325164|
Length = 2112 bp|
Definition = Oryza sativa Japonica Group Os01g0293100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:10712748..10714859|
CDS = 10713003..10713074,10713260..10713712,10713812..10714426|
GCID = <gbrowseImage1>
name=NC_008394:10712748..10714859
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:10712748..10714859
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgtatcacccgcagtgcgagctcctgatgccgcttgagagcctggagatggacgtcggccagtcgcacctcgccgccgccgtcgcagcagccatgccgggggagctcaacttccacctcctccactcgctcgacgccgccgcggcggctgcctcctccaccgccgcctcggcctcctcccagcccaccgtcgactacttcttcggcggcgccgaccagcagccgccgccgccggcggcgatgcagtacgaccagctggcggcgccgcaccaccaccagacggtggccatgctgcgcgactactacggcggccactacccgccggcggcggcggcggcggcggccaccgaggcgtacttccgcggcgggccaaggacggccgggtcgtcgtcgctcgtgttcggcccggccgacgacgagtcggccttcatggtcggacccttcgagagctccccgacgccgcggtccggcggcggcaggaagcgtagccgcgccaccgccggcttccacggcggcgggccggccaacggcgtcgagaagaaggagaagcagcgccgcctgcggctcaccgagaagtacaacgccctcatgctcctcatccccaaccgcaccaaggaggatagagcgacggtgatctcagacgcgatcgagtacatccaggagctagggaggacggtggaggagctgacgctgctggtggagaagaagcggcggcggagggagatgcagggggacgtggtggacgcggcgacgtcgtcggtggtggcggggatggatcaggcggcggagagctcggagggcgaggtgatggcggcggcggcgatgggcgcggtggcaccgccgccgcggcaggcgccgatccggagcacgtacatccagcggcggagcaaggagacgttcgtggacgtgcggatcgtggaggacgacgtgaacatcaagctcaccaagcgccgccgcgacggctgtctcgccgccgcgtcgcgcgcgctggacgacctccgcctcgacctcgtccacctctccggcggcaagatcggcgactgccacatctacatgttcaacaccaagattcattcgggatctccagtgtttgcaagtgcagtggccagcaggctgattgaagtggtggatgagtactaa</cdnaseq>|
AA = <aaseq>MYHPQCELLMPLESLEMDVGQSHLAAAVAAAMPGELNFHLLHSL DAAAAAASSTAASASSQPTVDYFFGGADQQPPPPAAMQYDQLAAPHHHQTVAMLRDYY GGHYPPAAAAAAATEAYFRGGPRTAGSSSLVFGPADDESAFMVGPFESSPTPRSGGGR KRSRATAGFHGGGPANGVEKKEKQRRLRLTEKYNALMLLIPNRTKEDRATVISDAIEY IQELGRTVEELTLLVEKKRRRREMQGDVVDAATSSVVAGMDQAAESSEGEVMAAAAMG AVAPPPRQAPIRSTYIQRRSKETFVDVRIVEDDVNIKLTKRRRDGCLAAASRALDDLR LDLVHLSGGKIGDCHIYMFNTKIHSGSPVFASAVASRLIEVVDEY</aaseq>|
DNA = <dnaseqindica>1786..1857#1148..1600#434..1048#aagaaaccaactgctttctcctacccaatatcacccttgccccttttatatactcttcctctcatcaccttctcgatcggcctctctcctctcctctcatcagctcacacccccaaccaacaaacctagttaatttagctctagttggttcatccctgctgcactgcgagctcaagtaatcgatctgagctctgaagaaaaaggtctgtttcaaattgaaccctttcatctcatcttctcattactgtttgattcattaattctagtaatttagagtttaaaccatcgcttttctttttgtgtgtgttcttgcatatgcttggacgattttgagtttgttctttaaatttgggcttgaacataggttgattaagcgagaagagtttcatggtttggattcttgatttgttgcaggtggtagagtgcgaggaagatgtatcacccgcagtgcgagctcctgatgccgcttgagagcctggagatggacgtcggccagtcgcacctcgccgccgccgtcgcagcagccatgccgggggagctcaacttccacctcctccactcgctcgacgccgccgcggcggctgcctcctccaccgccgcctcggcctcctcccagcccaccgtcgactacttcttcggcggcgccgaccagcagccgccgccgccggcggcgatgcagtacgaccagctggcggcgccgcaccaccaccagacggtggccatgctgcgcgactactacggcggccactacccgccggcggcggcggcggcggcggccaccgaggcgtacttccgcggcgggccaaggacggccgggtcgtcgtcgctcgtgttcggcccggccgacgacgagtcggccttcatggtcggacccttcgagagctccccgacgccgcggtccggcggcggcaggaagcgtagccgcgccaccgccggcttccacggcggcgggccggccaacggcgtcgagaagaaggagaagcagcgccgcctgcggctcaccgagaagtacaacgccctcatgctcctcatccccaaccgcaccaaggtatatcacatgccctagctaccatcgtcttcggtagttcgatctcttcttgtgttcatccatggatcgatcgatgatgattttgtctttctttttcaggaggatagagcgacggtgatctcagacgcgatcgagtacatccaggagctagggaggacggtggaggagctgacgctgctggtggagaagaagcggcggcggagggagatgcagggggacgtggtggacgcggcgacgtcgtcggtggtggcggggatggatcaggcggcggagagctcggagggcgaggtgatggcggcggcggcgatgggcgcggtggcaccgccgccgcggcaggcgccgatccggagcacgtacatccagcggcggagcaaggagacgttcgtggacgtgcggatcgtggaggacgacgtgaacatcaagctcaccaagcgccgccgcgacggctgtctcgccgccgcgtcgcgcgcgctggacgacctccgcctcgacctcgtccacctctccggcggcaagatcggcgactgccacatctacatgttcaacaccaaggtcacaacaaccaactaaaaatatccccgcgtttatctacaaaactaaccaatctcataatagtaattaattctagaacaattttatcgtgttgtgatcaacataaacctatatatgcaaataaattgctgctagttaatctcatcattgatttattattgtttatttggatgcgatgcatgcagattcattcgggatctccagtgtttgcaagtgcagtggccagcaggctgattgaagtggtggatgagtactaactagctcgagctagctaattagccgaccgaccgatcgatatgatgaaagtttctatgttgctagctagctagggttcttggatgcatgagtactgagtagctctttaattaatttccttttaattttagactgtttaatttggattggtaaagactcgtgttagcttttgggagatctttggtatgtcatggtttgcatgtattattttggtctacttggataaataattgatgctctttgagacgttaattaat</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049330.1 RefSeq:Os01g0293100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

File:Tip1.jpg

2014-06-05T11:53:11Z

Suchao13:

Os01g0293100

2014-06-05T11:52:07Z

Suchao13: /* Function */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.

TDR INTERACTING PROTEIN2 (TIP2) is a basic helix-loop-helix (bHLH) protein, functions as a crucial switch in the meristemoid transition and differentiation during early anther development.

* The tip2 mutants display undifferentiated inner three anther wall layers.

* The tip2 anthers have prolonged periclinal cell division activity after the formation of the inner two anther wall layers via periclinal cell division.

* The tip2 displayed undegenerated callose around meiocytes, suggesting defective callose degeneration in the tip2 mutant.

* The tip2 mutants display abort tapetal programmed cell death.

TIP2 acts upstream of TDR and EAT1 and directly regulates the expression of TDR and EAT1, suggested that the bHLH proteins TIP2, TDR, and EAT1 play
a central role in regulating differentiation, morphogenesis, and degradation of anther somatic cell layers.

[[File:tip1.jpg]]

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os01g0293100|
Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|
Version = NM_001049330.1 GI:115436073 GeneID:4325164|
Length = 2112 bp|
Definition = Oryza sativa Japonica Group Os01g0293100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
AP = Chromosome 1:10712748..10714859|
CDS = 10713003..10713074,10713260..10713712,10713812..10714426|
GCID = <gbrowseImage1>
name=NC_008394:10712748..10714859
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008394:10712748..10714859
source=RiceChromosome01
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgtatcacccgcagtgcgagctcctgatgccgcttgagagcctggagatggacgtcggccagtcgcacctcgccgccgccgtcgcagcagccatgccgggggagctcaacttccacctcctccactcgctcgacgccgccgcggcggctgcctcctccaccgccgcctcggcctcctcccagcccaccgtcgactacttcttcggcggcgccgaccagcagccgccgccgccggcggcgatgcagtacgaccagctggcggcgccgcaccaccaccagacggtggccatgctgcgcgactactacggcggccactacccgccggcggcggcggcggcggcggccaccgaggcgtacttccgcggcgggccaaggacggccgggtcgtcgtcgctcgtgttcggcccggccgacgacgagtcggccttcatggtcggacccttcgagagctccccgacgccgcggtccggcggcggcaggaagcgtagccgcgccaccgccggcttccacggcggcgggccggccaacggcgtcgagaagaaggagaagcagcgccgcctgcggctcaccgagaagtacaacgccctcatgctcctcatccccaaccgcaccaaggaggatagagcgacggtgatctcagacgcgatcgagtacatccaggagctagggaggacggtggaggagctgacgctgctggtggagaagaagcggcggcggagggagatgcagggggacgtggtggacgcggcgacgtcgtcggtggtggcggggatggatcaggcggcggagagctcggagggcgaggtgatggcggcggcggcgatgggcgcggtggcaccgccgccgcggcaggcgccgatccggagcacgtacatccagcggcggagcaaggagacgttcgtggacgtgcggatcgtggaggacgacgtgaacatcaagctcaccaagcgccgccgcgacggctgtctcgccgccgcgtcgcgcgcgctggacgacctccgcctcgacctcgtccacctctccggcggcaagatcggcgactgccacatctacatgttcaacaccaagattcattcgggatctccagtgtttgcaagtgcagtggccagcaggctgattgaagtggtggatgagtactaa</cdnaseq>|
AA = <aaseq>MYHPQCELLMPLESLEMDVGQSHLAAAVAAAMPGELNFHLLHSL DAAAAAASSTAASASSQPTVDYFFGGADQQPPPPAAMQYDQLAAPHHHQTVAMLRDYY GGHYPPAAAAAAATEAYFRGGPRTAGSSSLVFGPADDESAFMVGPFESSPTPRSGGGR KRSRATAGFHGGGPANGVEKKEKQRRLRLTEKYNALMLLIPNRTKEDRATVISDAIEY IQELGRTVEELTLLVEKKRRRREMQGDVVDAATSSVVAGMDQAAESSEGEVMAAAAMG AVAPPPRQAPIRSTYIQRRSKETFVDVRIVEDDVNIKLTKRRRDGCLAAASRALDDLR LDLVHLSGGKIGDCHIYMFNTKIHSGSPVFASAVASRLIEVVDEY</aaseq>|
DNA = <dnaseqindica>1786..1857#1148..1600#434..1048#aagaaaccaactgctttctcctacccaatatcacccttgccccttttatatactcttcctctcatcaccttctcgatcggcctctctcctctcctctcatcagctcacacccccaaccaacaaacctagttaatttagctctagttggttcatccctgctgcactgcgagctcaagtaatcgatctgagctctgaagaaaaaggtctgtttcaaattgaaccctttcatctcatcttctcattactgtttgattcattaattctagtaatttagagtttaaaccatcgcttttctttttgtgtgtgttcttgcatatgcttggacgattttgagtttgttctttaaatttgggcttgaacataggttgattaagcgagaagagtttcatggtttggattcttgatttgttgcaggtggtagagtgcgaggaagatgtatcacccgcagtgcgagctcctgatgccgcttgagagcctggagatggacgtcggccagtcgcacctcgccgccgccgtcgcagcagccatgccgggggagctcaacttccacctcctccactcgctcgacgccgccgcggcggctgcctcctccaccgccgcctcggcctcctcccagcccaccgtcgactacttcttcggcggcgccgaccagcagccgccgccgccggcggcgatgcagtacgaccagctggcggcgccgcaccaccaccagacggtggccatgctgcgcgactactacggcggccactacccgccggcggcggcggcggcggcggccaccgaggcgtacttccgcggcgggccaaggacggccgggtcgtcgtcgctcgtgttcggcccggccgacgacgagtcggccttcatggtcggacccttcgagagctccccgacgccgcggtccggcggcggcaggaagcgtagccgcgccaccgccggcttccacggcggcgggccggccaacggcgtcgagaagaaggagaagcagcgccgcctgcggctcaccgagaagtacaacgccctcatgctcctcatccccaaccgcaccaaggtatatcacatgccctagctaccatcgtcttcggtagttcgatctcttcttgtgttcatccatggatcgatcgatgatgattttgtctttctttttcaggaggatagagcgacggtgatctcagacgcgatcgagtacatccaggagctagggaggacggtggaggagctgacgctgctggtggagaagaagcggcggcggagggagatgcagggggacgtggtggacgcggcgacgtcgtcggtggtggcggggatggatcaggcggcggagagctcggagggcgaggtgatggcggcggcggcgatgggcgcggtggcaccgccgccgcggcaggcgccgatccggagcacgtacatccagcggcggagcaaggagacgttcgtggacgtgcggatcgtggaggacgacgtgaacatcaagctcaccaagcgccgccgcgacggctgtctcgccgccgcgtcgcgcgcgctggacgacctccgcctcgacctcgtccacctctccggcggcaagatcggcgactgccacatctacatgttcaacaccaaggtcacaacaaccaactaaaaatatccccgcgtttatctacaaaactaaccaatctcataatagtaattaattctagaacaattttatcgtgttgtgatcaacataaacctatatatgcaaataaattgctgctagttaatctcatcattgatttattattgtttatttggatgcgatgcatgcagattcattcgggatctccagtgtttgcaagtgcagtggccagcaggctgattgaagtggtggatgagtactaactagctcgagctagctaattagccgaccgaccgatcgatatgatgaaagtttctatgttgctagctagctagggttcttggatgcatgagtactgagtagctctttaattaatttccttttaattttagactgtttaatttggattggtaaagactcgtgttagcttttgggagatctttggtatgtcatggtttgcatgtattattttggtctacttggataaataattgatgctctttgagacgttaattaat</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049330.1 RefSeq:Os01g0293100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 1]]
[[Category:Chromosome 1]]

Os04g0653000

2014-06-04T08:42:26Z

Suchao13: /* References */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.

* EG2(extra glume 2)is a JA signalling repressor that interacts with a putative JA receptor, OsCOI1b, to trigger OsJAZ1’s degradation during spikelet development.

* Its high sequence similarity with the Arabidopsis JAZ proteins so also called OsJAZ1.

*It belong to TIFY family so also called TIFY family.

===Expression===
Please input expression information here.

EG2 expressed ubiquitously in the plant, from roots, stems and leaves, to spikelets and seeds.
[[File:eg-1.jpg]]

===Mutation and Phenotype===
Please input information here.

The EG2 gene was mapped to chromosome 4 between CQQ2 and CQQ3, two In-Del markers that define a 2.2 cM.

[[File:eg-2.jpg]]

The mutation of EG2 is eg2-1D, which caused by C-to-G transition, resulting in a substitution of Ala by Gly in the fifth amino acid of the Jas domain. This motif was known to be critical for JAZ’s interaction with the JA receptor COI1.

[[File:eg-3.jpg]]

mutants displayed defects in spikelet development,including extra glume-like structures between the sterile lemma and lemma and altered floral organ numbers and identities.

[[File:eg-4.jpg]][[File:eg-5.jpg]]

===EG2 is a repressor of JA response===
Please input information here.

yeast two-hybrid (Y2H) assays show OsJAZ1 can interact with OsCOI1b, and the interaction was dependent on JA-Ile’s mimic, coronatine(COR), but not JA or MeJA. No interaction was observed between OsJAZ1 and OsCOI1a or OsCOI1c.

[[File:eg-6.jpg]]

Furthermore, the Ala-to-Gly mutation in the Jas domain of OsJAZ1 in eg2-1D blocked the interact between OsJAZ1 and JOsCOI1b, suggesting that the Jas domain is required for the interaction between OsJAZ1 and OsCOI1b. because osjaz1-1D can't interact with OsCOI1b, so it can't be JA-dependent proteasome-mediated degradation.

[[File:eg-7.jpg]]

JA regulates early spikelet development possibly by activating the expression of OsMADS1 through OsMYC2, which is directly regulates OsMADS1 expression.And, OsJAZ1 interacts with OsMYC2 and suppresses the activity of OsMYC2 in triggering the transcription of OsMADS1.Both OsJAZ1 and osjaz1-1D interacted with OsMYC2, and the interaction required the Jas domain.

Inconclude,eg2-1D can't interact with OsCOI1b, so it not degradation, but it also can interact with OsMYC2 to block OsMYC2 regulates the OsMADS1 expression.

[[File:eg-9.jpg]]

===Extend knowledge===
Please input information here.

The TIFY family is a novel plant-specific gene family involved in the regulation of diverse plant-specific biologic processes. This report identifies 20 TIFY genes in rice, On the basis of their protein structures, members of the TIFY family can be divided into two groups. Almost all the OsTIFY genes were responsive to one or more abiotic stresses including drought, salinity, and low temperature.Suggesting that the OsTIFY family may have important roles in response to
abiotic stresses.

To analyze the evolutionary relationships of the OsTIFY genes, a phylogenetic tree was generated using the sequence alignments of 38 full-length TIFY proteins,
including 20 from rice and 18 from Arabidopsis thaliana. The result show in the picture.

[[File:eg-10.jpg]]

==Labs working on this gene==
Please input related labs here.

*State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University

*Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University

*Department of Energy Plant Research Laboratory, Michigan State University

*National Key Laboratory of Crop Genetic Improvement,National Center of Plant Gene Research,Huazhong Agricultural University

==References==
Please input cited references here.

* Qiang Cai, Zheng Yuan, Mingjiao Chen, et al. Jasmonic acid regulates spikelet development in rice. NATURE COMMUNICATIONS, 2014

* Haiyan Ye, Hao Du, Ning Tang, et al. Identification and expression profiling analysis of TIFY family genes involved in stress and phytohormone responses in rice. Plant Mol Biol, 2009

==Structured Information==
{{JaponicaGene|
GeneName = Os04g0653000|
Description = The start codon is not identified.|
Version = NM_001060638.1 GI:115461005 GeneID:4337240|
Length = 3445 bp|
Definition = Oryza sativa Japonica Group Os04g0653000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|
AP = Chromosome 4:33705210..33708654|
CDS = 33705211..33705317,33707373..33707475,33707567..33707792,33707906..33707963,33708275..33708365 |
GCID = <gbrowseImage1>
name=NC_008397:33705210..33708654
source=RiceChromosome04
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008397:33705210..33708654
source=RiceChromosome04
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>ggagaggagaggaaggaggaagaggtggtggtggaggagaagagccatcagcagcagcagcagcaaggggaggaggagctcgtcggcctctcgctcgccggcggcaggcccaaagtatttccaatgtcgagccctccacctaatccttcgcaacttacaattttctatggtggatcagtatgtgtttatgactcagtgccgccagagaaggctcaagcaatcatgcttattgccgcagcagctgctgcagcggcgtctgccaccaaaagcaatgctgccattgctgttaagccccctgtgatgcctgcagccaatgctacccaagcagcagtctctcctgtgctcacaagatcgctctcattgcagagcacttctgtagcaactggacaacctcaagttgccgctgaccctagctcaatttgcaagctccaggctgatctccccattgccaggaggcactcccttcagcgcttcctagagaaacgccgtgacaggttggtgagcaaagctccatatcccacaaaatcctccgagggcatggaagcatcagggatggaagtaactgctgagggcaaggcccagtaa</cdnaseq>|
AA = <aaseq>GEERKEEEVVVEEKSHQQQQQQGEEELVGLSLAGGRPKVFPMSS PPPNPSQLTIFYGGSVCVYDSVPPEKAQAIMLIAAAAAAAASATKSNAAIAVKPPVMP AANATQAAVSPVLTRSLSLQSTSVATGQPQVAADPSSICKLQADLPIARRHSLQRFLE KRRDRLVSKAPYPTKSSEGMEASGMEVTAEGKAQ</aaseq>|
DNA = <dnaseqindica>2..108#2164..2266#2358..2583#2697..2754#3066..3156#gggagaggagaggaaggaggaagaggtggtggtggaggagaagagccatcagcagcagcagcagcaaggggaggaggagctcgtcggcctctcgctcgccggcggcaggtagttttccggctccgccttctcctctcgcaagtcgagtcctattcagctttgggttgatcgattgggtcgcgggagagagagtatttggttggttgttcgggaaatgcatctcgcgcgggattctagccgcttttcagttcatagtatcgccccttctccgagaactcgccgccggggagccgcttcctgctgggagccgagcggcgccgcagccgcctccgaagtggtttaccggaggggatggattttctccggtactggactcctggtgtttgaatttttgggtggaaccggagactctttttttggtcgggtgaaattccttggaccgaccaaaaaaatctcgaatccactcggagtttcctccgttccagatggagctcttgcgttgaaaattttcctaaaaatagagtcaaaatgtttcgacttcgagttaagagtccaccctacaccgagttgcaactttcccttttttacgagttggtgtaatcatggaagagcccttagctttgctgcagtggatcaaattggtgggggttcttgcctaggtcatgtgaagattcttctgattagtggtagtctcatgcttctcctcggcctgcaatccaaatcccctttattcctttcccgctctcttgcgcttggcgtcagctgttcttaaccttgaacaaattagggtgtcattctagttgcacgtgttcttatccagatgttgggagtgcggtgaactgctgatattctactaaaccgatatcaattatatggatgttcaatttggttcacgtcactaggtaaatgatttcccaccttagaccaaacttaacggataggaaagaacttttggcaagtggcagatccaggtagtttttttccttccctttttctgtaattggcagttacttgttctccaaatacttggaactgctgtgaaaagtttccaattgagccttggaatcaggagcaatggggaactcttctaagttacttgcatttcttctttaaagactcagaaataataagcaagaaacacacatagatctggtgaaacttgagttagcgtgcagtttttaacacctccatgtaccaaggggattcaatggtaaaggcctcatccccttgtcttatctttacttcaagactaaatctgtagctggggaccaagtaccatagtttcgcaactcttatataatccactaggctgctgtgaaaacaatagatgcagtgtttgttttgaagtaagtaaggtgaaagtaaaaatacctggtaatggctaatatgaatcgtggctattattaaatgtttcagcattttctgaataggtaaaccacatatatgtaactatgtaagatgtttttaaatctagatgttctattgagcctgtaacctggtcttttagactagattatgctgagactttgaagaaaaaaatttcagtacattccgtccatcttggacaacctttggacagattattaactctgaatcagtcccatcatgctgcacatgttggatatttcagagtggaccataaagttgcaggcctagtttgctatttttagattttagatcttgtgagttttaatcttgatcatgctattagactcagaatgtgtgtaccaaaaacattgataatggtatctatcctgacttgggtataacttgtgttactggcagctgtgggagggctatgttggcgtagcgtactgaccaagagataatcctctcatcaagtagaatatagagagtagaataattatttatgaaggagatgggcctttatctggctgcagacaaggtgcatagtaaagctcattaaacctctaaaaaaggctggaaatacggcctacggtgtaattgcacattatacatcagttagctaggttggcctccaattttatatggcactttacccttggatgcccatcttatgtattgccatgtactcatgtatgcaattagtcaagaacggcccacttatgccaatatctgtgtaactatgcaatctgttattaccttcttgcacatttactaatttcgctttgtcataatcaggcccaaagtatttccaatgtcgagccctccacctaatccttcgcaacttacaattttctatggtggatcagtatgtgtttatgactcagtgccgccagagaaggtcatattcaccccacttatcttcgttatatcctccgactaactgttgaaagtttatgctaattcgttatctgcaacattgtcatcaacaggctcaagcaatcatgcttattgccgcagcagctgctgcagcggcgtctgccaccaaaagcaatgctgccattgctgttaagccccctgtgatgcctgcagccaatgctacccaagcagcagtctctcctgtgctcacaagatcgctctcattgcagagcacttctgtagcaactggacaacctcaagttgccgctgaccctagctcaatttgcaagctccaggctggtaagactacaagatctttttgtgaatctaaaatggggctgttttaatccctcgtcatatgatgtactaagtgattgaagatgctacaaatttattctttatttgctgtgcagatctccccattgccaggaggcactcccttcagcgcttcctagagaaacgccgtgacaggttggcatgaatgaatgtagaaatagcattctatatttgtctgatgcatttccttaggtttagtggtccattagaacatgctaaaagaatgtggatagctggaaactgacttgtaggggataaaacatttgctttagcttgacttggatacgaaccaagtatatgtgcaaggactgttttcagtttcattaccatagcccatgttttcatgatacataacaaagtagccagtagttatagaaacacagctttctgcaaatttgagaaatccttagcttcaactgattaagccaaatgaccatgttaagcaggttggtgagcaaagctccatatcccacaaaatcctccgagggcatggaagcatcagggatggaagtaactgctgagggcaaggcccagtaactgtcaacttcgaatggtggaggctgacgtttggagggggaaggaaggaaaggcgtcgcgttcattgtttagatggtgtcgcaccctagaaccttactaataatcgaacctatgcccttattatcgttgtactgttccttatgtgctagtctattctgtcatgaatttgtttactcgaacagcaaactgctatgatagtagtatatcattgtacatcatgcatatcattgcctgacaatgttatcgtgttcgtctagtaatgatatcatccaggttaattgttactagt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001060638.1 RefSeq:Os04g0653000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 4]]
[[Category:Chromosome 4]]

Os04g0653000

2014-06-04T08:38:38Z

Suchao13: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.

* EG2(extra glume 2)is a JA signalling repressor that interacts with a putative JA receptor, OsCOI1b, to trigger OsJAZ1’s degradation during spikelet development.

* Its high sequence similarity with the Arabidopsis JAZ proteins so also called OsJAZ1.

*It belong to TIFY family so also called TIFY family.

===Expression===
Please input expression information here.

EG2 expressed ubiquitously in the plant, from roots, stems and leaves, to spikelets and seeds.
[[File:eg-1.jpg]]

===Mutation and Phenotype===
Please input information here.

The EG2 gene was mapped to chromosome 4 between CQQ2 and CQQ3, two In-Del markers that define a 2.2 cM.

[[File:eg-2.jpg]]

The mutation of EG2 is eg2-1D, which caused by C-to-G transition, resulting in a substitution of Ala by Gly in the fifth amino acid of the Jas domain. This motif was known to be critical for JAZ’s interaction with the JA receptor COI1.

[[File:eg-3.jpg]]

mutants displayed defects in spikelet development,including extra glume-like structures between the sterile lemma and lemma and altered floral organ numbers and identities.

[[File:eg-4.jpg]][[File:eg-5.jpg]]

===EG2 is a repressor of JA response===
Please input information here.

yeast two-hybrid (Y2H) assays show OsJAZ1 can interact with OsCOI1b, and the interaction was dependent on JA-Ile’s mimic, coronatine(COR), but not JA or MeJA. No interaction was observed between OsJAZ1 and OsCOI1a or OsCOI1c.

[[File:eg-6.jpg]]

Furthermore, the Ala-to-Gly mutation in the Jas domain of OsJAZ1 in eg2-1D blocked the interact between OsJAZ1 and JOsCOI1b, suggesting that the Jas domain is required for the interaction between OsJAZ1 and OsCOI1b. because osjaz1-1D can't interact with OsCOI1b, so it can't be JA-dependent proteasome-mediated degradation.

[[File:eg-7.jpg]]

JA regulates early spikelet development possibly by activating the expression of OsMADS1 through OsMYC2, which is directly regulates OsMADS1 expression.And, OsJAZ1 interacts with OsMYC2 and suppresses the activity of OsMYC2 in triggering the transcription of OsMADS1.Both OsJAZ1 and osjaz1-1D interacted with OsMYC2, and the interaction required the Jas domain.

Inconclude,eg2-1D can't interact with OsCOI1b, so it not degradation, but it also can interact with OsMYC2 to block OsMYC2 regulates the OsMADS1 expression.

[[File:eg-9.jpg]]

===Extend knowledge===
Please input information here.

The TIFY family is a novel plant-specific gene family involved in the regulation of diverse plant-specific biologic processes. This report identifies 20 TIFY genes in rice, On the basis of their protein structures, members of the TIFY family can be divided into two groups. Almost all the OsTIFY genes were responsive to one or more abiotic stresses including drought, salinity, and low temperature.Suggesting that the OsTIFY family may have important roles in response to
abiotic stresses.

To analyze the evolutionary relationships of the OsTIFY genes, a phylogenetic tree was generated using the sequence alignments of 38 full-length TIFY proteins,
including 20 from rice and 18 from Arabidopsis thaliana. The result show in the picture.

[[File:eg-10.jpg]]

==Labs working on this gene==
Please input related labs here.

*State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University

*Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University

*Department of Energy Plant Research Laboratory, Michigan State University

*National Key Laboratory of Crop Genetic Improvement,National Center of Plant Gene Research,Huazhong Agricultural University

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os04g0653000|
Description = The start codon is not identified.|
Version = NM_001060638.1 GI:115461005 GeneID:4337240|
Length = 3445 bp|
Definition = Oryza sativa Japonica Group Os04g0653000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|
AP = Chromosome 4:33705210..33708654|
CDS = 33705211..33705317,33707373..33707475,33707567..33707792,33707906..33707963,33708275..33708365 |
GCID = <gbrowseImage1>
name=NC_008397:33705210..33708654
source=RiceChromosome04
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008397:33705210..33708654
source=RiceChromosome04
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>ggagaggagaggaaggaggaagaggtggtggtggaggagaagagccatcagcagcagcagcagcaaggggaggaggagctcgtcggcctctcgctcgccggcggcaggcccaaagtatttccaatgtcgagccctccacctaatccttcgcaacttacaattttctatggtggatcagtatgtgtttatgactcagtgccgccagagaaggctcaagcaatcatgcttattgccgcagcagctgctgcagcggcgtctgccaccaaaagcaatgctgccattgctgttaagccccctgtgatgcctgcagccaatgctacccaagcagcagtctctcctgtgctcacaagatcgctctcattgcagagcacttctgtagcaactggacaacctcaagttgccgctgaccctagctcaatttgcaagctccaggctgatctccccattgccaggaggcactcccttcagcgcttcctagagaaacgccgtgacaggttggtgagcaaagctccatatcccacaaaatcctccgagggcatggaagcatcagggatggaagtaactgctgagggcaaggcccagtaa</cdnaseq>|
AA = <aaseq>GEERKEEEVVVEEKSHQQQQQQGEEELVGLSLAGGRPKVFPMSS PPPNPSQLTIFYGGSVCVYDSVPPEKAQAIMLIAAAAAAAASATKSNAAIAVKPPVMP AANATQAAVSPVLTRSLSLQSTSVATGQPQVAADPSSICKLQADLPIARRHSLQRFLE KRRDRLVSKAPYPTKSSEGMEASGMEVTAEGKAQ</aaseq>|
DNA = <dnaseqindica>2..108#2164..2266#2358..2583#2697..2754#3066..3156#gggagaggagaggaaggaggaagaggtggtggtggaggagaagagccatcagcagcagcagcagcaaggggaggaggagctcgtcggcctctcgctcgccggcggcaggtagttttccggctccgccttctcctctcgcaagtcgagtcctattcagctttgggttgatcgattgggtcgcgggagagagagtatttggttggttgttcgggaaatgcatctcgcgcgggattctagccgcttttcagttcatagtatcgccccttctccgagaactcgccgccggggagccgcttcctgctgggagccgagcggcgccgcagccgcctccgaagtggtttaccggaggggatggattttctccggtactggactcctggtgtttgaatttttgggtggaaccggagactctttttttggtcgggtgaaattccttggaccgaccaaaaaaatctcgaatccactcggagtttcctccgttccagatggagctcttgcgttgaaaattttcctaaaaatagagtcaaaatgtttcgacttcgagttaagagtccaccctacaccgagttgcaactttcccttttttacgagttggtgtaatcatggaagagcccttagctttgctgcagtggatcaaattggtgggggttcttgcctaggtcatgtgaagattcttctgattagtggtagtctcatgcttctcctcggcctgcaatccaaatcccctttattcctttcccgctctcttgcgcttggcgtcagctgttcttaaccttgaacaaattagggtgtcattctagttgcacgtgttcttatccagatgttgggagtgcggtgaactgctgatattctactaaaccgatatcaattatatggatgttcaatttggttcacgtcactaggtaaatgatttcccaccttagaccaaacttaacggataggaaagaacttttggcaagtggcagatccaggtagtttttttccttccctttttctgtaattggcagttacttgttctccaaatacttggaactgctgtgaaaagtttccaattgagccttggaatcaggagcaatggggaactcttctaagttacttgcatttcttctttaaagactcagaaataataagcaagaaacacacatagatctggtgaaacttgagttagcgtgcagtttttaacacctccatgtaccaaggggattcaatggtaaaggcctcatccccttgtcttatctttacttcaagactaaatctgtagctggggaccaagtaccatagtttcgcaactcttatataatccactaggctgctgtgaaaacaatagatgcagtgtttgttttgaagtaagtaaggtgaaagtaaaaatacctggtaatggctaatatgaatcgtggctattattaaatgtttcagcattttctgaataggtaaaccacatatatgtaactatgtaagatgtttttaaatctagatgttctattgagcctgtaacctggtcttttagactagattatgctgagactttgaagaaaaaaatttcagtacattccgtccatcttggacaacctttggacagattattaactctgaatcagtcccatcatgctgcacatgttggatatttcagagtggaccataaagttgcaggcctagtttgctatttttagattttagatcttgtgagttttaatcttgatcatgctattagactcagaatgtgtgtaccaaaaacattgataatggtatctatcctgacttgggtataacttgtgttactggcagctgtgggagggctatgttggcgtagcgtactgaccaagagataatcctctcatcaagtagaatatagagagtagaataattatttatgaaggagatgggcctttatctggctgcagacaaggtgcatagtaaagctcattaaacctctaaaaaaggctggaaatacggcctacggtgtaattgcacattatacatcagttagctaggttggcctccaattttatatggcactttacccttggatgcccatcttatgtattgccatgtactcatgtatgcaattagtcaagaacggcccacttatgccaatatctgtgtaactatgcaatctgttattaccttcttgcacatttactaatttcgctttgtcataatcaggcccaaagtatttccaatgtcgagccctccacctaatccttcgcaacttacaattttctatggtggatcagtatgtgtttatgactcagtgccgccagagaaggtcatattcaccccacttatcttcgttatatcctccgactaactgttgaaagtttatgctaattcgttatctgcaacattgtcatcaacaggctcaagcaatcatgcttattgccgcagcagctgctgcagcggcgtctgccaccaaaagcaatgctgccattgctgttaagccccctgtgatgcctgcagccaatgctacccaagcagcagtctctcctgtgctcacaagatcgctctcattgcagagcacttctgtagcaactggacaacctcaagttgccgctgaccctagctcaatttgcaagctccaggctggtaagactacaagatctttttgtgaatctaaaatggggctgttttaatccctcgtcatatgatgtactaagtgattgaagatgctacaaatttattctttatttgctgtgcagatctccccattgccaggaggcactcccttcagcgcttcctagagaaacgccgtgacaggttggcatgaatgaatgtagaaatagcattctatatttgtctgatgcatttccttaggtttagtggtccattagaacatgctaaaagaatgtggatagctggaaactgacttgtaggggataaaacatttgctttagcttgacttggatacgaaccaagtatatgtgcaaggactgttttcagtttcattaccatagcccatgttttcatgatacataacaaagtagccagtagttatagaaacacagctttctgcaaatttgagaaatccttagcttcaactgattaagccaaatgaccatgttaagcaggttggtgagcaaagctccatatcccacaaaatcctccgagggcatggaagcatcagggatggaagtaactgctgagggcaaggcccagtaactgtcaacttcgaatggtggaggctgacgtttggagggggaaggaaggaaaggcgtcgcgttcattgtttagatggtgtcgcaccctagaaccttactaataatcgaacctatgcccttattatcgttgtactgttccttatgtgctagtctattctgtcatgaatttgtttactcgaacagcaaactgctatgatagtagtatatcattgtacatcatgcatatcattgcctgacaatgttatcgtgttcgtctagtaatgatatcatccaggttaattgttactagt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001060638.1 RefSeq:Os04g0653000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 4]]
[[Category:Chromosome 4]]

Os04g0653000

2014-06-04T08:36:51Z

Suchao13: /* Function */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.

* EG2(extra glume 2)is a JA signalling repressor that interacts with a putative JA receptor, OsCOI1b, to trigger OsJAZ1’s degradation during spikelet development.

* Its high sequence similarity with the Arabidopsis JAZ proteins so also called OsJAZ1.

*It belong to TIFY family so also called TIFY family.

===Expression===
Please input expression information here.

EG2 expressed ubiquitously in the plant, from roots, stems and leaves, to spikelets and seeds.
[[File:eg-1.jpg]]

===Mutation and Phenotype===
Please input information here.

The EG2 gene was mapped to chromosome 4 between CQQ2 and CQQ3, two In-Del markers that define a 2.2 cM.

[[File:eg-2.jpg]]

The mutation of EG2 is eg2-1D, which caused by C-to-G transition, resulting in a substitution of Ala by Gly in the fifth amino acid of the Jas domain. This motif was known to be critical for JAZ’s interaction with the JA receptor COI1.

[[File:eg-3.jpg]]

mutants displayed defects in spikelet development,including extra glume-like structures between the sterile lemma and lemma and altered floral organ numbers and identities.

[[File:eg-4.jpg]][[File:eg-5.jpg]]

===EG2 is a repressor of JA response===
Please input information here.

yeast two-hybrid (Y2H) assays show OsJAZ1 can interact with OsCOI1b, and the interaction was dependent on JA-Ile’s mimic, coronatine(COR), but not JA or MeJA. No interaction was observed between OsJAZ1 and OsCOI1a or OsCOI1c.

[[File:eg-6.jpg]]

Furthermore, the Ala-to-Gly mutation in the Jas domain of OsJAZ1 in eg2-1D blocked the interact between OsJAZ1 and JOsCOI1b, suggesting that the Jas domain is required for the interaction between OsJAZ1 and OsCOI1b. because osjaz1-1D can't interact with OsCOI1b, so it can't be JA-dependent proteasome-mediated degradation.

[[File:eg-7.jpg]]

JA regulates early spikelet development possibly by activating the expression of OsMADS1 through OsMYC2, which is directly regulates OsMADS1 expression.And, OsJAZ1 interacts with OsMYC2 and suppresses the activity of OsMYC2 in triggering the transcription of OsMADS1.Both OsJAZ1 and osjaz1-1D interacted with OsMYC2, and the interaction required the Jas domain.

Inconclude,eg2-1D can't interact with OsCOI1b, so it not degradation, but it also can interact with OsMYC2 to block OsMYC2 regulates the OsMADS1 expression.

[[File:eg-9.jpg]]

===Extend knowledge===
Please input information here.

The TIFY family is a novel plant-specific gene family involved in the regulation of diverse plant-specific biologic processes. This report identifies 20 TIFY genes in rice, On the basis of their protein structures, members of the TIFY family can be divided into two groups. Almost all the OsTIFY genes were responsive to one or more abiotic stresses including drought, salinity, and low temperature.Suggesting that the OsTIFY family may have important roles in response to
abiotic stresses.

To analyze the evolutionary relationships of the OsTIFY genes, a phylogenetic tree was generated using the sequence alignments of 38 full-length TIFY proteins,
including 20 from rice and 18 from Arabidopsis thaliana. The result show in the picture.

[[File:eg-10.jpg]]

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os04g0653000|
Description = The start codon is not identified.|
Version = NM_001060638.1 GI:115461005 GeneID:4337240|
Length = 3445 bp|
Definition = Oryza sativa Japonica Group Os04g0653000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|
AP = Chromosome 4:33705210..33708654|
CDS = 33705211..33705317,33707373..33707475,33707567..33707792,33707906..33707963,33708275..33708365 |
GCID = <gbrowseImage1>
name=NC_008397:33705210..33708654
source=RiceChromosome04
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008397:33705210..33708654
source=RiceChromosome04
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>ggagaggagaggaaggaggaagaggtggtggtggaggagaagagccatcagcagcagcagcagcaaggggaggaggagctcgtcggcctctcgctcgccggcggcaggcccaaagtatttccaatgtcgagccctccacctaatccttcgcaacttacaattttctatggtggatcagtatgtgtttatgactcagtgccgccagagaaggctcaagcaatcatgcttattgccgcagcagctgctgcagcggcgtctgccaccaaaagcaatgctgccattgctgttaagccccctgtgatgcctgcagccaatgctacccaagcagcagtctctcctgtgctcacaagatcgctctcattgcagagcacttctgtagcaactggacaacctcaagttgccgctgaccctagctcaatttgcaagctccaggctgatctccccattgccaggaggcactcccttcagcgcttcctagagaaacgccgtgacaggttggtgagcaaagctccatatcccacaaaatcctccgagggcatggaagcatcagggatggaagtaactgctgagggcaaggcccagtaa</cdnaseq>|
AA = <aaseq>GEERKEEEVVVEEKSHQQQQQQGEEELVGLSLAGGRPKVFPMSS PPPNPSQLTIFYGGSVCVYDSVPPEKAQAIMLIAAAAAAAASATKSNAAIAVKPPVMP AANATQAAVSPVLTRSLSLQSTSVATGQPQVAADPSSICKLQADLPIARRHSLQRFLE KRRDRLVSKAPYPTKSSEGMEASGMEVTAEGKAQ</aaseq>|
DNA = <dnaseqindica>2..108#2164..2266#2358..2583#2697..2754#3066..3156#gggagaggagaggaaggaggaagaggtggtggtggaggagaagagccatcagcagcagcagcagcaaggggaggaggagctcgtcggcctctcgctcgccggcggcaggtagttttccggctccgccttctcctctcgcaagtcgagtcctattcagctttgggttgatcgattgggtcgcgggagagagagtatttggttggttgttcgggaaatgcatctcgcgcgggattctagccgcttttcagttcatagtatcgccccttctccgagaactcgccgccggggagccgcttcctgctgggagccgagcggcgccgcagccgcctccgaagtggtttaccggaggggatggattttctccggtactggactcctggtgtttgaatttttgggtggaaccggagactctttttttggtcgggtgaaattccttggaccgaccaaaaaaatctcgaatccactcggagtttcctccgttccagatggagctcttgcgttgaaaattttcctaaaaatagagtcaaaatgtttcgacttcgagttaagagtccaccctacaccgagttgcaactttcccttttttacgagttggtgtaatcatggaagagcccttagctttgctgcagtggatcaaattggtgggggttcttgcctaggtcatgtgaagattcttctgattagtggtagtctcatgcttctcctcggcctgcaatccaaatcccctttattcctttcccgctctcttgcgcttggcgtcagctgttcttaaccttgaacaaattagggtgtcattctagttgcacgtgttcttatccagatgttgggagtgcggtgaactgctgatattctactaaaccgatatcaattatatggatgttcaatttggttcacgtcactaggtaaatgatttcccaccttagaccaaacttaacggataggaaagaacttttggcaagtggcagatccaggtagtttttttccttccctttttctgtaattggcagttacttgttctccaaatacttggaactgctgtgaaaagtttccaattgagccttggaatcaggagcaatggggaactcttctaagttacttgcatttcttctttaaagactcagaaataataagcaagaaacacacatagatctggtgaaacttgagttagcgtgcagtttttaacacctccatgtaccaaggggattcaatggtaaaggcctcatccccttgtcttatctttacttcaagactaaatctgtagctggggaccaagtaccatagtttcgcaactcttatataatccactaggctgctgtgaaaacaatagatgcagtgtttgttttgaagtaagtaaggtgaaagtaaaaatacctggtaatggctaatatgaatcgtggctattattaaatgtttcagcattttctgaataggtaaaccacatatatgtaactatgtaagatgtttttaaatctagatgttctattgagcctgtaacctggtcttttagactagattatgctgagactttgaagaaaaaaatttcagtacattccgtccatcttggacaacctttggacagattattaactctgaatcagtcccatcatgctgcacatgttggatatttcagagtggaccataaagttgcaggcctagtttgctatttttagattttagatcttgtgagttttaatcttgatcatgctattagactcagaatgtgtgtaccaaaaacattgataatggtatctatcctgacttgggtataacttgtgttactggcagctgtgggagggctatgttggcgtagcgtactgaccaagagataatcctctcatcaagtagaatatagagagtagaataattatttatgaaggagatgggcctttatctggctgcagacaaggtgcatagtaaagctcattaaacctctaaaaaaggctggaaatacggcctacggtgtaattgcacattatacatcagttagctaggttggcctccaattttatatggcactttacccttggatgcccatcttatgtattgccatgtactcatgtatgcaattagtcaagaacggcccacttatgccaatatctgtgtaactatgcaatctgttattaccttcttgcacatttactaatttcgctttgtcataatcaggcccaaagtatttccaatgtcgagccctccacctaatccttcgcaacttacaattttctatggtggatcagtatgtgtttatgactcagtgccgccagagaaggtcatattcaccccacttatcttcgttatatcctccgactaactgttgaaagtttatgctaattcgttatctgcaacattgtcatcaacaggctcaagcaatcatgcttattgccgcagcagctgctgcagcggcgtctgccaccaaaagcaatgctgccattgctgttaagccccctgtgatgcctgcagccaatgctacccaagcagcagtctctcctgtgctcacaagatcgctctcattgcagagcacttctgtagcaactggacaacctcaagttgccgctgaccctagctcaatttgcaagctccaggctggtaagactacaagatctttttgtgaatctaaaatggggctgttttaatccctcgtcatatgatgtactaagtgattgaagatgctacaaatttattctttatttgctgtgcagatctccccattgccaggaggcactcccttcagcgcttcctagagaaacgccgtgacaggttggcatgaatgaatgtagaaatagcattctatatttgtctgatgcatttccttaggtttagtggtccattagaacatgctaaaagaatgtggatagctggaaactgacttgtaggggataaaacatttgctttagcttgacttggatacgaaccaagtatatgtgcaaggactgttttcagtttcattaccatagcccatgttttcatgatacataacaaagtagccagtagttatagaaacacagctttctgcaaatttgagaaatccttagcttcaactgattaagccaaatgaccatgttaagcaggttggtgagcaaagctccatatcccacaaaatcctccgagggcatggaagcatcagggatggaagtaactgctgagggcaaggcccagtaactgtcaacttcgaatggtggaggctgacgtttggagggggaaggaaggaaaggcgtcgcgttcattgtttagatggtgtcgcaccctagaaccttactaataatcgaacctatgcccttattatcgttgtactgttccttatgtgctagtctattctgtcatgaatttgtttactcgaacagcaaactgctatgatagtagtatatcattgtacatcatgcatatcattgcctgacaatgttatcgtgttcgtctagtaatgatatcatccaggttaattgttactagt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001060638.1 RefSeq:Os04g0653000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 4]]
[[Category:Chromosome 4]]

File:Eg-10.jpg

2014-06-04T08:36:27Z

Suchao13:

Os04g0653000

2014-06-04T08:35:13Z

Suchao13: /* Extend knowledge */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.

* EG2(extra glume 2)is a JA signalling repressor that interacts with a putative JA receptor, OsCOI1b, to trigger OsJAZ1’s degradation during spikelet development[1].

* Its high sequence similarity with the Arabidopsis JAZ proteins so also called OsJAZ1[1].

*It belong to TIFY family so also called TIFY family[2].

===Expression===
Please input expression information here.

EG2 expressed ubiquitously in the plant, from roots, stems and leaves, to spikelets and seeds.
[[File:eg-1.jpg]]

===Mutation and Phenotype===
Please input information here.

The EG2 gene was mapped to chromosome 4 between CQQ2 and CQQ3, two In-Del markers that define a 2.2 cM.

[[File:eg-2.jpg]]

The mutation of EG2 is eg2-1D, which caused by C-to-G transition, resulting in a substitution of Ala by Gly in the fifth amino acid of the Jas domain. This motif was known to be critical for JAZ’s interaction with the JA receptor COI1.

[[File:eg-3.jpg]]

mutants displayed defects in spikelet development,including extra glume-like structures between the sterile lemma and lemma and altered floral organ numbers and identities.

[[File:eg-4.jpg]][[File:eg-5.jpg]]

===EG2 is a repressor of JA response===
Please input information here.

yeast two-hybrid (Y2H) assays show OsJAZ1 can interact with OsCOI1b, and the interaction was dependent on JA-Ile’s mimic, coronatine(COR), but not JA or MeJA. No interaction was observed between OsJAZ1 and OsCOI1a or OsCOI1c.

[[File:eg-6.jpg]]

Furthermore, the Ala-to-Gly mutation in the Jas domain of OsJAZ1 in eg2-1D blocked the interact between OsJAZ1 and JOsCOI1b, suggesting that the Jas domain is required for the interaction between OsJAZ1 and OsCOI1b. because osjaz1-1D can't interact with OsCOI1b, so it can't be JA-dependent proteasome-mediated degradation.

[[File:eg-7.jpg]]

JA regulates early spikelet development possibly by activating the expression of OsMADS1 through OsMYC2, which is directly regulates OsMADS1 expression.And, OsJAZ1 interacts with OsMYC2 and suppresses the activity of OsMYC2 in triggering the transcription of OsMADS1.Both OsJAZ1 and osjaz1-1D interacted with OsMYC2, and the interaction required the Jas domain.

Inconclude,eg2-1D can't interact with OsCOI1b, so it not degradation, but it also can interact with OsMYC2 to block OsMYC2 regulates the OsMADS1 expression.

[[File:eg-9.jpg]]

===Extend knowledge===
Please input information here.

The TIFY family is a novel plant-specific gene family involved in the regulation of diverse plant-specific biologic processes. This report identifies 20 TIFY genes in rice, On the basis of their protein structures, members of the TIFY family can be divided into two groups. Almost all the OsTIFY genes were responsive to one or more abiotic stresses including drought, salinity, and low temperature.Suggesting that the OsTIFY family may have important roles in response to
abiotic stresses.

To analyze the evolutionary relationships of the OsTIFY genes, a phylogenetic tree was generated using the sequence alignments of 38 full-length TIFY proteins,
including 20 from rice and 18 from Arabidopsis thaliana. The result show in the picture.

[[File:eg-10.jpg]]

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os04g0653000|
Description = The start codon is not identified.|
Version = NM_001060638.1 GI:115461005 GeneID:4337240|
Length = 3445 bp|
Definition = Oryza sativa Japonica Group Os04g0653000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|
AP = Chromosome 4:33705210..33708654|
CDS = 33705211..33705317,33707373..33707475,33707567..33707792,33707906..33707963,33708275..33708365 |
GCID = <gbrowseImage1>
name=NC_008397:33705210..33708654
source=RiceChromosome04
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008397:33705210..33708654
source=RiceChromosome04
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>ggagaggagaggaaggaggaagaggtggtggtggaggagaagagccatcagcagcagcagcagcaaggggaggaggagctcgtcggcctctcgctcgccggcggcaggcccaaagtatttccaatgtcgagccctccacctaatccttcgcaacttacaattttctatggtggatcagtatgtgtttatgactcagtgccgccagagaaggctcaagcaatcatgcttattgccgcagcagctgctgcagcggcgtctgccaccaaaagcaatgctgccattgctgttaagccccctgtgatgcctgcagccaatgctacccaagcagcagtctctcctgtgctcacaagatcgctctcattgcagagcacttctgtagcaactggacaacctcaagttgccgctgaccctagctcaatttgcaagctccaggctgatctccccattgccaggaggcactcccttcagcgcttcctagagaaacgccgtgacaggttggtgagcaaagctccatatcccacaaaatcctccgagggcatggaagcatcagggatggaagtaactgctgagggcaaggcccagtaa</cdnaseq>|
AA = <aaseq>GEERKEEEVVVEEKSHQQQQQQGEEELVGLSLAGGRPKVFPMSS PPPNPSQLTIFYGGSVCVYDSVPPEKAQAIMLIAAAAAAAASATKSNAAIAVKPPVMP AANATQAAVSPVLTRSLSLQSTSVATGQPQVAADPSSICKLQADLPIARRHSLQRFLE KRRDRLVSKAPYPTKSSEGMEASGMEVTAEGKAQ</aaseq>|
DNA = <dnaseqindica>2..108#2164..2266#2358..2583#2697..2754#3066..3156#gggagaggagaggaaggaggaagaggtggtggtggaggagaagagccatcagcagcagcagcagcaaggggaggaggagctcgtcggcctctcgctcgccggcggcaggtagttttccggctccgccttctcctctcgcaagtcgagtcctattcagctttgggttgatcgattgggtcgcgggagagagagtatttggttggttgttcgggaaatgcatctcgcgcgggattctagccgcttttcagttcatagtatcgccccttctccgagaactcgccgccggggagccgcttcctgctgggagccgagcggcgccgcagccgcctccgaagtggtttaccggaggggatggattttctccggtactggactcctggtgtttgaatttttgggtggaaccggagactctttttttggtcgggtgaaattccttggaccgaccaaaaaaatctcgaatccactcggagtttcctccgttccagatggagctcttgcgttgaaaattttcctaaaaatagagtcaaaatgtttcgacttcgagttaagagtccaccctacaccgagttgcaactttcccttttttacgagttggtgtaatcatggaagagcccttagctttgctgcagtggatcaaattggtgggggttcttgcctaggtcatgtgaagattcttctgattagtggtagtctcatgcttctcctcggcctgcaatccaaatcccctttattcctttcccgctctcttgcgcttggcgtcagctgttcttaaccttgaacaaattagggtgtcattctagttgcacgtgttcttatccagatgttgggagtgcggtgaactgctgatattctactaaaccgatatcaattatatggatgttcaatttggttcacgtcactaggtaaatgatttcccaccttagaccaaacttaacggataggaaagaacttttggcaagtggcagatccaggtagtttttttccttccctttttctgtaattggcagttacttgttctccaaatacttggaactgctgtgaaaagtttccaattgagccttggaatcaggagcaatggggaactcttctaagttacttgcatttcttctttaaagactcagaaataataagcaagaaacacacatagatctggtgaaacttgagttagcgtgcagtttttaacacctccatgtaccaaggggattcaatggtaaaggcctcatccccttgtcttatctttacttcaagactaaatctgtagctggggaccaagtaccatagtttcgcaactcttatataatccactaggctgctgtgaaaacaatagatgcagtgtttgttttgaagtaagtaaggtgaaagtaaaaatacctggtaatggctaatatgaatcgtggctattattaaatgtttcagcattttctgaataggtaaaccacatatatgtaactatgtaagatgtttttaaatctagatgttctattgagcctgtaacctggtcttttagactagattatgctgagactttgaagaaaaaaatttcagtacattccgtccatcttggacaacctttggacagattattaactctgaatcagtcccatcatgctgcacatgttggatatttcagagtggaccataaagttgcaggcctagtttgctatttttagattttagatcttgtgagttttaatcttgatcatgctattagactcagaatgtgtgtaccaaaaacattgataatggtatctatcctgacttgggtataacttgtgttactggcagctgtgggagggctatgttggcgtagcgtactgaccaagagataatcctctcatcaagtagaatatagagagtagaataattatttatgaaggagatgggcctttatctggctgcagacaaggtgcatagtaaagctcattaaacctctaaaaaaggctggaaatacggcctacggtgtaattgcacattatacatcagttagctaggttggcctccaattttatatggcactttacccttggatgcccatcttatgtattgccatgtactcatgtatgcaattagtcaagaacggcccacttatgccaatatctgtgtaactatgcaatctgttattaccttcttgcacatttactaatttcgctttgtcataatcaggcccaaagtatttccaatgtcgagccctccacctaatccttcgcaacttacaattttctatggtggatcagtatgtgtttatgactcagtgccgccagagaaggtcatattcaccccacttatcttcgttatatcctccgactaactgttgaaagtttatgctaattcgttatctgcaacattgtcatcaacaggctcaagcaatcatgcttattgccgcagcagctgctgcagcggcgtctgccaccaaaagcaatgctgccattgctgttaagccccctgtgatgcctgcagccaatgctacccaagcagcagtctctcctgtgctcacaagatcgctctcattgcagagcacttctgtagcaactggacaacctcaagttgccgctgaccctagctcaatttgcaagctccaggctggtaagactacaagatctttttgtgaatctaaaatggggctgttttaatccctcgtcatatgatgtactaagtgattgaagatgctacaaatttattctttatttgctgtgcagatctccccattgccaggaggcactcccttcagcgcttcctagagaaacgccgtgacaggttggcatgaatgaatgtagaaatagcattctatatttgtctgatgcatttccttaggtttagtggtccattagaacatgctaaaagaatgtggatagctggaaactgacttgtaggggataaaacatttgctttagcttgacttggatacgaaccaagtatatgtgcaaggactgttttcagtttcattaccatagcccatgttttcatgatacataacaaagtagccagtagttatagaaacacagctttctgcaaatttgagaaatccttagcttcaactgattaagccaaatgaccatgttaagcaggttggtgagcaaagctccatatcccacaaaatcctccgagggcatggaagcatcagggatggaagtaactgctgagggcaaggcccagtaactgtcaacttcgaatggtggaggctgacgtttggagggggaaggaaggaaaggcgtcgcgttcattgtttagatggtgtcgcaccctagaaccttactaataatcgaacctatgcccttattatcgttgtactgttccttatgtgctagtctattctgtcatgaatttgtttactcgaacagcaaactgctatgatagtagtatatcattgtacatcatgcatatcattgcctgacaatgttatcgtgttcgtctagtaatgatatcatccaggttaattgttactagt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001060638.1 RefSeq:Os04g0653000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 4]]
[[Category:Chromosome 4]]

Os04g0653000

2014-06-04T08:28:21Z

Suchao13: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.

* EG2(extra glume 2)is a JA signalling repressor that interacts with a putative JA receptor, OsCOI1b, to trigger OsJAZ1’s degradation during spikelet development[1].

* Its high sequence similarity with the Arabidopsis JAZ proteins so also called OsJAZ1[1].

*It belong to TIFY family so also called TIFY family[2].

===Expression===
Please input expression information here.

EG2 expressed ubiquitously in the plant, from roots, stems and leaves, to spikelets and seeds.
[[File:eg-1.jpg]]

===Mutation and Phenotype===
Please input information here.

The EG2 gene was mapped to chromosome 4 between CQQ2 and CQQ3, two In-Del markers that define a 2.2 cM.

[[File:eg-2.jpg]]

The mutation of EG2 is eg2-1D, which caused by C-to-G transition, resulting in a substitution of Ala by Gly in the fifth amino acid of the Jas domain. This motif was known to be critical for JAZ’s interaction with the JA receptor COI1.

[[File:eg-3.jpg]]

mutants displayed defects in spikelet development,including extra glume-like structures between the sterile lemma and lemma and altered floral organ numbers and identities.

[[File:eg-4.jpg]][[File:eg-5.jpg]]

===EG2 is a repressor of JA response===
Please input information here.

yeast two-hybrid (Y2H) assays show OsJAZ1 can interact with OsCOI1b, and the interaction was dependent on JA-Ile’s mimic, coronatine(COR), but not JA or MeJA. No interaction was observed between OsJAZ1 and OsCOI1a or OsCOI1c.

[[File:eg-6.jpg]]

Furthermore, the Ala-to-Gly mutation in the Jas domain of OsJAZ1 in eg2-1D blocked the interact between OsJAZ1 and JOsCOI1b, suggesting that the Jas domain is required for the interaction between OsJAZ1 and OsCOI1b. because osjaz1-1D can't interact with OsCOI1b, so it can't be JA-dependent proteasome-mediated degradation.

[[File:eg-7.jpg]]

JA regulates early spikelet development possibly by activating the expression of OsMADS1 through OsMYC2, which is directly regulates OsMADS1 expression.And, OsJAZ1 interacts with OsMYC2 and suppresses the activity of OsMYC2 in triggering the transcription of OsMADS1.Both OsJAZ1 and osjaz1-1D interacted with OsMYC2, and the interaction required the Jas domain.

Inconclude,eg2-1D can't interact with OsCOI1b, so it not degradation, but it also can interact with OsMYC2 to block OsMYC2 regulates the OsMADS1 expression.

[[File:eg-9.jpg]]

===Extend knowledge===
Please input information here.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os04g0653000|
Description = The start codon is not identified.|
Version = NM_001060638.1 GI:115461005 GeneID:4337240|
Length = 3445 bp|
Definition = Oryza sativa Japonica Group Os04g0653000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|
AP = Chromosome 4:33705210..33708654|
CDS = 33705211..33705317,33707373..33707475,33707567..33707792,33707906..33707963,33708275..33708365 |
GCID = <gbrowseImage1>
name=NC_008397:33705210..33708654
source=RiceChromosome04
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008397:33705210..33708654
source=RiceChromosome04
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>ggagaggagaggaaggaggaagaggtggtggtggaggagaagagccatcagcagcagcagcagcaaggggaggaggagctcgtcggcctctcgctcgccggcggcaggcccaaagtatttccaatgtcgagccctccacctaatccttcgcaacttacaattttctatggtggatcagtatgtgtttatgactcagtgccgccagagaaggctcaagcaatcatgcttattgccgcagcagctgctgcagcggcgtctgccaccaaaagcaatgctgccattgctgttaagccccctgtgatgcctgcagccaatgctacccaagcagcagtctctcctgtgctcacaagatcgctctcattgcagagcacttctgtagcaactggacaacctcaagttgccgctgaccctagctcaatttgcaagctccaggctgatctccccattgccaggaggcactcccttcagcgcttcctagagaaacgccgtgacaggttggtgagcaaagctccatatcccacaaaatcctccgagggcatggaagcatcagggatggaagtaactgctgagggcaaggcccagtaa</cdnaseq>|
AA = <aaseq>GEERKEEEVVVEEKSHQQQQQQGEEELVGLSLAGGRPKVFPMSS PPPNPSQLTIFYGGSVCVYDSVPPEKAQAIMLIAAAAAAAASATKSNAAIAVKPPVMP AANATQAAVSPVLTRSLSLQSTSVATGQPQVAADPSSICKLQADLPIARRHSLQRFLE KRRDRLVSKAPYPTKSSEGMEASGMEVTAEGKAQ</aaseq>|
DNA = <dnaseqindica>2..108#2164..2266#2358..2583#2697..2754#3066..3156#gggagaggagaggaaggaggaagaggtggtggtggaggagaagagccatcagcagcagcagcagcaaggggaggaggagctcgtcggcctctcgctcgccggcggcaggtagttttccggctccgccttctcctctcgcaagtcgagtcctattcagctttgggttgatcgattgggtcgcgggagagagagtatttggttggttgttcgggaaatgcatctcgcgcgggattctagccgcttttcagttcatagtatcgccccttctccgagaactcgccgccggggagccgcttcctgctgggagccgagcggcgccgcagccgcctccgaagtggtttaccggaggggatggattttctccggtactggactcctggtgtttgaatttttgggtggaaccggagactctttttttggtcgggtgaaattccttggaccgaccaaaaaaatctcgaatccactcggagtttcctccgttccagatggagctcttgcgttgaaaattttcctaaaaatagagtcaaaatgtttcgacttcgagttaagagtccaccctacaccgagttgcaactttcccttttttacgagttggtgtaatcatggaagagcccttagctttgctgcagtggatcaaattggtgggggttcttgcctaggtcatgtgaagattcttctgattagtggtagtctcatgcttctcctcggcctgcaatccaaatcccctttattcctttcccgctctcttgcgcttggcgtcagctgttcttaaccttgaacaaattagggtgtcattctagttgcacgtgttcttatccagatgttgggagtgcggtgaactgctgatattctactaaaccgatatcaattatatggatgttcaatttggttcacgtcactaggtaaatgatttcccaccttagaccaaacttaacggataggaaagaacttttggcaagtggcagatccaggtagtttttttccttccctttttctgtaattggcagttacttgttctccaaatacttggaactgctgtgaaaagtttccaattgagccttggaatcaggagcaatggggaactcttctaagttacttgcatttcttctttaaagactcagaaataataagcaagaaacacacatagatctggtgaaacttgagttagcgtgcagtttttaacacctccatgtaccaaggggattcaatggtaaaggcctcatccccttgtcttatctttacttcaagactaaatctgtagctggggaccaagtaccatagtttcgcaactcttatataatccactaggctgctgtgaaaacaatagatgcagtgtttgttttgaagtaagtaaggtgaaagtaaaaatacctggtaatggctaatatgaatcgtggctattattaaatgtttcagcattttctgaataggtaaaccacatatatgtaactatgtaagatgtttttaaatctagatgttctattgagcctgtaacctggtcttttagactagattatgctgagactttgaagaaaaaaatttcagtacattccgtccatcttggacaacctttggacagattattaactctgaatcagtcccatcatgctgcacatgttggatatttcagagtggaccataaagttgcaggcctagtttgctatttttagattttagatcttgtgagttttaatcttgatcatgctattagactcagaatgtgtgtaccaaaaacattgataatggtatctatcctgacttgggtataacttgtgttactggcagctgtgggagggctatgttggcgtagcgtactgaccaagagataatcctctcatcaagtagaatatagagagtagaataattatttatgaaggagatgggcctttatctggctgcagacaaggtgcatagtaaagctcattaaacctctaaaaaaggctggaaatacggcctacggtgtaattgcacattatacatcagttagctaggttggcctccaattttatatggcactttacccttggatgcccatcttatgtattgccatgtactcatgtatgcaattagtcaagaacggcccacttatgccaatatctgtgtaactatgcaatctgttattaccttcttgcacatttactaatttcgctttgtcataatcaggcccaaagtatttccaatgtcgagccctccacctaatccttcgcaacttacaattttctatggtggatcagtatgtgtttatgactcagtgccgccagagaaggtcatattcaccccacttatcttcgttatatcctccgactaactgttgaaagtttatgctaattcgttatctgcaacattgtcatcaacaggctcaagcaatcatgcttattgccgcagcagctgctgcagcggcgtctgccaccaaaagcaatgctgccattgctgttaagccccctgtgatgcctgcagccaatgctacccaagcagcagtctctcctgtgctcacaagatcgctctcattgcagagcacttctgtagcaactggacaacctcaagttgccgctgaccctagctcaatttgcaagctccaggctggtaagactacaagatctttttgtgaatctaaaatggggctgttttaatccctcgtcatatgatgtactaagtgattgaagatgctacaaatttattctttatttgctgtgcagatctccccattgccaggaggcactcccttcagcgcttcctagagaaacgccgtgacaggttggcatgaatgaatgtagaaatagcattctatatttgtctgatgcatttccttaggtttagtggtccattagaacatgctaaaagaatgtggatagctggaaactgacttgtaggggataaaacatttgctttagcttgacttggatacgaaccaagtatatgtgcaaggactgttttcagtttcattaccatagcccatgttttcatgatacataacaaagtagccagtagttatagaaacacagctttctgcaaatttgagaaatccttagcttcaactgattaagccaaatgaccatgttaagcaggttggtgagcaaagctccatatcccacaaaatcctccgagggcatggaagcatcagggatggaagtaactgctgagggcaaggcccagtaactgtcaacttcgaatggtggaggctgacgtttggagggggaaggaaggaaaggcgtcgcgttcattgtttagatggtgtcgcaccctagaaccttactaataatcgaacctatgcccttattatcgttgtactgttccttatgtgctagtctattctgtcatgaatttgtttactcgaacagcaaactgctatgatagtagtatatcattgtacatcatgcatatcattgcctgacaatgttatcgtgttcgtctagtaatgatatcatccaggttaattgttactagt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001060638.1 RefSeq:Os04g0653000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 4]]
[[Category:Chromosome 4]]

File:Eg-9.jpg

2014-06-04T08:25:58Z

Suchao13:

Os04g0653000

2014-06-04T08:25:44Z

Suchao13: /* EG2 is a repressor of JA response */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.

* EG2(extra glume 2)is a JA signalling repressor that interacts with a putative JA receptor, OsCOI1b, to trigger OsJAZ1’s degradation during spikelet development[1].

* Its high sequence similarity with the Arabidopsis JAZ proteins so also called OsJAZ1[1].

*It belong to TIFY family so also called TIFY family[2].

===Expression===
Please input expression information here.

EG2 expressed ubiquitously in the plant, from roots, stems and leaves, to spikelets and seeds.
[[File:eg-1.jpg]]

===Mutation and Phenotype===
Please input information here.

The EG2 gene was mapped to chromosome 4 between CQQ2 and CQQ3, two In-Del markers that define a 2.2 cM.

[[File:eg-2.jpg]]

The mutation of EG2 is eg2-1D, which caused by C-to-G transition, resulting in a substitution of Ala by Gly in the fifth amino acid of the Jas domain. This motif was known to be critical for JAZ’s interaction with the JA receptor COI1.

[[File:eg-3.jpg]]

mutants displayed defects in spikelet development,including extra glume-like structures between the sterile lemma and lemma and altered floral organ numbers and identities.

[[File:eg-4.jpg]][[File:eg-5.jpg]]

===EG2 is a repressor of JA response===
Please input information here.

yeast two-hybrid (Y2H) assays show OsJAZ1 can interact with OsCOI1b, and the interaction was dependent on JA-Ile’s mimic, coronatine(COR), but not JA or MeJA. No interaction was observed between OsJAZ1 and OsCOI1a or OsCOI1c.

[[File:eg-6.jpg]]

Furthermore, the Ala-to-Gly mutation in the Jas domain of OsJAZ1 in eg2-1D blocked the interact between OsJAZ1 and JOsCOI1b, suggesting that the Jas domain is required for the interaction between OsJAZ1 and OsCOI1b. because osjaz1-1D can't interact with OsCOI1b, so it can't be JA-dependent proteasome-mediated degradation.

[[File:eg-7.jpg]]

JA regulates early spikelet development possibly by activating the expression of OsMADS1 through OsMYC2, which is directly regulates OsMADS1 expression.And, OsJAZ1 interacts with OsMYC2 and suppresses the activity of OsMYC2 in triggering the transcription of OsMADS1.Both OsJAZ1 and osjaz1-1D interacted with OsMYC2, and the interaction required the Jas domain.

Inconclude,eg2-1D can't interact with OsCOI1b, so it not degradation, but it also can interact with OsMYC2 to block OsMYC2 regulates the OsMADS1 expression.

[[File:eg-9.jpg]]

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os04g0653000|
Description = The start codon is not identified.|
Version = NM_001060638.1 GI:115461005 GeneID:4337240|
Length = 3445 bp|
Definition = Oryza sativa Japonica Group Os04g0653000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|
AP = Chromosome 4:33705210..33708654|
CDS = 33705211..33705317,33707373..33707475,33707567..33707792,33707906..33707963,33708275..33708365 |
GCID = <gbrowseImage1>
name=NC_008397:33705210..33708654
source=RiceChromosome04
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008397:33705210..33708654
source=RiceChromosome04
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>ggagaggagaggaaggaggaagaggtggtggtggaggagaagagccatcagcagcagcagcagcaaggggaggaggagctcgtcggcctctcgctcgccggcggcaggcccaaagtatttccaatgtcgagccctccacctaatccttcgcaacttacaattttctatggtggatcagtatgtgtttatgactcagtgccgccagagaaggctcaagcaatcatgcttattgccgcagcagctgctgcagcggcgtctgccaccaaaagcaatgctgccattgctgttaagccccctgtgatgcctgcagccaatgctacccaagcagcagtctctcctgtgctcacaagatcgctctcattgcagagcacttctgtagcaactggacaacctcaagttgccgctgaccctagctcaatttgcaagctccaggctgatctccccattgccaggaggcactcccttcagcgcttcctagagaaacgccgtgacaggttggtgagcaaagctccatatcccacaaaatcctccgagggcatggaagcatcagggatggaagtaactgctgagggcaaggcccagtaa</cdnaseq>|
AA = <aaseq>GEERKEEEVVVEEKSHQQQQQQGEEELVGLSLAGGRPKVFPMSS PPPNPSQLTIFYGGSVCVYDSVPPEKAQAIMLIAAAAAAAASATKSNAAIAVKPPVMP AANATQAAVSPVLTRSLSLQSTSVATGQPQVAADPSSICKLQADLPIARRHSLQRFLE KRRDRLVSKAPYPTKSSEGMEASGMEVTAEGKAQ</aaseq>|
DNA = <dnaseqindica>2..108#2164..2266#2358..2583#2697..2754#3066..3156#gggagaggagaggaaggaggaagaggtggtggtggaggagaagagccatcagcagcagcagcagcaaggggaggaggagctcgtcggcctctcgctcgccggcggcaggtagttttccggctccgccttctcctctcgcaagtcgagtcctattcagctttgggttgatcgattgggtcgcgggagagagagtatttggttggttgttcgggaaatgcatctcgcgcgggattctagccgcttttcagttcatagtatcgccccttctccgagaactcgccgccggggagccgcttcctgctgggagccgagcggcgccgcagccgcctccgaagtggtttaccggaggggatggattttctccggtactggactcctggtgtttgaatttttgggtggaaccggagactctttttttggtcgggtgaaattccttggaccgaccaaaaaaatctcgaatccactcggagtttcctccgttccagatggagctcttgcgttgaaaattttcctaaaaatagagtcaaaatgtttcgacttcgagttaagagtccaccctacaccgagttgcaactttcccttttttacgagttggtgtaatcatggaagagcccttagctttgctgcagtggatcaaattggtgggggttcttgcctaggtcatgtgaagattcttctgattagtggtagtctcatgcttctcctcggcctgcaatccaaatcccctttattcctttcccgctctcttgcgcttggcgtcagctgttcttaaccttgaacaaattagggtgtcattctagttgcacgtgttcttatccagatgttgggagtgcggtgaactgctgatattctactaaaccgatatcaattatatggatgttcaatttggttcacgtcactaggtaaatgatttcccaccttagaccaaacttaacggataggaaagaacttttggcaagtggcagatccaggtagtttttttccttccctttttctgtaattggcagttacttgttctccaaatacttggaactgctgtgaaaagtttccaattgagccttggaatcaggagcaatggggaactcttctaagttacttgcatttcttctttaaagactcagaaataataagcaagaaacacacatagatctggtgaaacttgagttagcgtgcagtttttaacacctccatgtaccaaggggattcaatggtaaaggcctcatccccttgtcttatctttacttcaagactaaatctgtagctggggaccaagtaccatagtttcgcaactcttatataatccactaggctgctgtgaaaacaatagatgcagtgtttgttttgaagtaagtaaggtgaaagtaaaaatacctggtaatggctaatatgaatcgtggctattattaaatgtttcagcattttctgaataggtaaaccacatatatgtaactatgtaagatgtttttaaatctagatgttctattgagcctgtaacctggtcttttagactagattatgctgagactttgaagaaaaaaatttcagtacattccgtccatcttggacaacctttggacagattattaactctgaatcagtcccatcatgctgcacatgttggatatttcagagtggaccataaagttgcaggcctagtttgctatttttagattttagatcttgtgagttttaatcttgatcatgctattagactcagaatgtgtgtaccaaaaacattgataatggtatctatcctgacttgggtataacttgtgttactggcagctgtgggagggctatgttggcgtagcgtactgaccaagagataatcctctcatcaagtagaatatagagagtagaataattatttatgaaggagatgggcctttatctggctgcagacaaggtgcatagtaaagctcattaaacctctaaaaaaggctggaaatacggcctacggtgtaattgcacattatacatcagttagctaggttggcctccaattttatatggcactttacccttggatgcccatcttatgtattgccatgtactcatgtatgcaattagtcaagaacggcccacttatgccaatatctgtgtaactatgcaatctgttattaccttcttgcacatttactaatttcgctttgtcataatcaggcccaaagtatttccaatgtcgagccctccacctaatccttcgcaacttacaattttctatggtggatcagtatgtgtttatgactcagtgccgccagagaaggtcatattcaccccacttatcttcgttatatcctccgactaactgttgaaagtttatgctaattcgttatctgcaacattgtcatcaacaggctcaagcaatcatgcttattgccgcagcagctgctgcagcggcgtctgccaccaaaagcaatgctgccattgctgttaagccccctgtgatgcctgcagccaatgctacccaagcagcagtctctcctgtgctcacaagatcgctctcattgcagagcacttctgtagcaactggacaacctcaagttgccgctgaccctagctcaatttgcaagctccaggctggtaagactacaagatctttttgtgaatctaaaatggggctgttttaatccctcgtcatatgatgtactaagtgattgaagatgctacaaatttattctttatttgctgtgcagatctccccattgccaggaggcactcccttcagcgcttcctagagaaacgccgtgacaggttggcatgaatgaatgtagaaatagcattctatatttgtctgatgcatttccttaggtttagtggtccattagaacatgctaaaagaatgtggatagctggaaactgacttgtaggggataaaacatttgctttagcttgacttggatacgaaccaagtatatgtgcaaggactgttttcagtttcattaccatagcccatgttttcatgatacataacaaagtagccagtagttatagaaacacagctttctgcaaatttgagaaatccttagcttcaactgattaagccaaatgaccatgttaagcaggttggtgagcaaagctccatatcccacaaaatcctccgagggcatggaagcatcagggatggaagtaactgctgagggcaaggcccagtaactgtcaacttcgaatggtggaggctgacgtttggagggggaaggaaggaaaggcgtcgcgttcattgtttagatggtgtcgcaccctagaaccttactaataatcgaacctatgcccttattatcgttgtactgttccttatgtgctagtctattctgtcatgaatttgtttactcgaacagcaaactgctatgatagtagtatatcattgtacatcatgcatatcattgcctgacaatgttatcgtgttcgtctagtaatgatatcatccaggttaattgttactagt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001060638.1 RefSeq:Os04g0653000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 4]]
[[Category:Chromosome 4]]

File:Eg-7.jpg

2014-06-04T08:23:59Z

Suchao13:

File:Eg-6.jpg

2014-06-04T08:23:35Z

Suchao13:

Os04g0653000

2014-06-04T08:23:18Z

Suchao13: /* EG2 is a repressor of JA response */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.

* EG2(extra glume 2)is a JA signalling repressor that interacts with a putative JA receptor, OsCOI1b, to trigger OsJAZ1’s degradation during spikelet development[1].

* Its high sequence similarity with the Arabidopsis JAZ proteins so also called OsJAZ1[1].

*It belong to TIFY family so also called TIFY family[2].

===Expression===
Please input expression information here.

EG2 expressed ubiquitously in the plant, from roots, stems and leaves, to spikelets and seeds.
[[File:eg-1.jpg]]

===Mutation and Phenotype===
Please input information here.

The EG2 gene was mapped to chromosome 4 between CQQ2 and CQQ3, two In-Del markers that define a 2.2 cM.

[[File:eg-2.jpg]]

The mutation of EG2 is eg2-1D, which caused by C-to-G transition, resulting in a substitution of Ala by Gly in the fifth amino acid of the Jas domain. This motif was known to be critical for JAZ’s interaction with the JA receptor COI1.

[[File:eg-3.jpg]]

mutants displayed defects in spikelet development,including extra glume-like structures between the sterile lemma and lemma and altered floral organ numbers and identities.

[[File:eg-4.jpg]][[File:eg-5.jpg]]

===EG2 is a repressor of JA response===
Please input information here.

yeast two-hybrid (Y2H) assays show OsJAZ1 can interact with OsCOI1b, and the interaction was dependent on JA-Ile’s mimic, coronatine(COR), but not JA or MeJA. No interaction was observed between OsJAZ1 and OsCOI1a or OsCOI1c.

[[File:eg-6.jpg]]

Furthermore, the Ala-to-Gly mutation in the Jas domain of OsJAZ1 in eg2-1D blocked the interact between OsJAZ1 and JOsCOI1b, suggesting that the Jas domain is required for the interaction between OsJAZ1 and OsCOI1b. because osjaz1-1D can't interact with OsCOI1b, so it can't be JA-dependent proteasome-mediated degradation.

[[File:eg-7.jpg]]

JA regulates early spikelet development possibly by activating the expression of OsMADS1 through OsMYC2, which is directly regulates OsMADS1 expression.And, OsJAZ1 interacts with OsMYC2 and suppresses the activity of OsMYC2 in triggering the transcription of OsMADS1.Both OsJAZ1 and osjaz1-1D interacted with OsMYC2, and the interaction required the Jas domain.

[[File:eg-8.jpg]]

Inconclude,eg2-1D can't interact with OsCOI1b, so it not degradation, but it also can interact with OsMYC2 to block OsMYC2 regulates the OsMADS1 expression.

[[File:eg-9.jpg]]

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os04g0653000|
Description = The start codon is not identified.|
Version = NM_001060638.1 GI:115461005 GeneID:4337240|
Length = 3445 bp|
Definition = Oryza sativa Japonica Group Os04g0653000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|
AP = Chromosome 4:33705210..33708654|
CDS = 33705211..33705317,33707373..33707475,33707567..33707792,33707906..33707963,33708275..33708365 |
GCID = <gbrowseImage1>
name=NC_008397:33705210..33708654
source=RiceChromosome04
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008397:33705210..33708654
source=RiceChromosome04
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>ggagaggagaggaaggaggaagaggtggtggtggaggagaagagccatcagcagcagcagcagcaaggggaggaggagctcgtcggcctctcgctcgccggcggcaggcccaaagtatttccaatgtcgagccctccacctaatccttcgcaacttacaattttctatggtggatcagtatgtgtttatgactcagtgccgccagagaaggctcaagcaatcatgcttattgccgcagcagctgctgcagcggcgtctgccaccaaaagcaatgctgccattgctgttaagccccctgtgatgcctgcagccaatgctacccaagcagcagtctctcctgtgctcacaagatcgctctcattgcagagcacttctgtagcaactggacaacctcaagttgccgctgaccctagctcaatttgcaagctccaggctgatctccccattgccaggaggcactcccttcagcgcttcctagagaaacgccgtgacaggttggtgagcaaagctccatatcccacaaaatcctccgagggcatggaagcatcagggatggaagtaactgctgagggcaaggcccagtaa</cdnaseq>|
AA = <aaseq>GEERKEEEVVVEEKSHQQQQQQGEEELVGLSLAGGRPKVFPMSS PPPNPSQLTIFYGGSVCVYDSVPPEKAQAIMLIAAAAAAAASATKSNAAIAVKPPVMP AANATQAAVSPVLTRSLSLQSTSVATGQPQVAADPSSICKLQADLPIARRHSLQRFLE KRRDRLVSKAPYPTKSSEGMEASGMEVTAEGKAQ</aaseq>|
DNA = <dnaseqindica>2..108#2164..2266#2358..2583#2697..2754#3066..3156#gggagaggagaggaaggaggaagaggtggtggtggaggagaagagccatcagcagcagcagcagcaaggggaggaggagctcgtcggcctctcgctcgccggcggcaggtagttttccggctccgccttctcctctcgcaagtcgagtcctattcagctttgggttgatcgattgggtcgcgggagagagagtatttggttggttgttcgggaaatgcatctcgcgcgggattctagccgcttttcagttcatagtatcgccccttctccgagaactcgccgccggggagccgcttcctgctgggagccgagcggcgccgcagccgcctccgaagtggtttaccggaggggatggattttctccggtactggactcctggtgtttgaatttttgggtggaaccggagactctttttttggtcgggtgaaattccttggaccgaccaaaaaaatctcgaatccactcggagtttcctccgttccagatggagctcttgcgttgaaaattttcctaaaaatagagtcaaaatgtttcgacttcgagttaagagtccaccctacaccgagttgcaactttcccttttttacgagttggtgtaatcatggaagagcccttagctttgctgcagtggatcaaattggtgggggttcttgcctaggtcatgtgaagattcttctgattagtggtagtctcatgcttctcctcggcctgcaatccaaatcccctttattcctttcccgctctcttgcgcttggcgtcagctgttcttaaccttgaacaaattagggtgtcattctagttgcacgtgttcttatccagatgttgggagtgcggtgaactgctgatattctactaaaccgatatcaattatatggatgttcaatttggttcacgtcactaggtaaatgatttcccaccttagaccaaacttaacggataggaaagaacttttggcaagtggcagatccaggtagtttttttccttccctttttctgtaattggcagttacttgttctccaaatacttggaactgctgtgaaaagtttccaattgagccttggaatcaggagcaatggggaactcttctaagttacttgcatttcttctttaaagactcagaaataataagcaagaaacacacatagatctggtgaaacttgagttagcgtgcagtttttaacacctccatgtaccaaggggattcaatggtaaaggcctcatccccttgtcttatctttacttcaagactaaatctgtagctggggaccaagtaccatagtttcgcaactcttatataatccactaggctgctgtgaaaacaatagatgcagtgtttgttttgaagtaagtaaggtgaaagtaaaaatacctggtaatggctaatatgaatcgtggctattattaaatgtttcagcattttctgaataggtaaaccacatatatgtaactatgtaagatgtttttaaatctagatgttctattgagcctgtaacctggtcttttagactagattatgctgagactttgaagaaaaaaatttcagtacattccgtccatcttggacaacctttggacagattattaactctgaatcagtcccatcatgctgcacatgttggatatttcagagtggaccataaagttgcaggcctagtttgctatttttagattttagatcttgtgagttttaatcttgatcatgctattagactcagaatgtgtgtaccaaaaacattgataatggtatctatcctgacttgggtataacttgtgttactggcagctgtgggagggctatgttggcgtagcgtactgaccaagagataatcctctcatcaagtagaatatagagagtagaataattatttatgaaggagatgggcctttatctggctgcagacaaggtgcatagtaaagctcattaaacctctaaaaaaggctggaaatacggcctacggtgtaattgcacattatacatcagttagctaggttggcctccaattttatatggcactttacccttggatgcccatcttatgtattgccatgtactcatgtatgcaattagtcaagaacggcccacttatgccaatatctgtgtaactatgcaatctgttattaccttcttgcacatttactaatttcgctttgtcataatcaggcccaaagtatttccaatgtcgagccctccacctaatccttcgcaacttacaattttctatggtggatcagtatgtgtttatgactcagtgccgccagagaaggtcatattcaccccacttatcttcgttatatcctccgactaactgttgaaagtttatgctaattcgttatctgcaacattgtcatcaacaggctcaagcaatcatgcttattgccgcagcagctgctgcagcggcgtctgccaccaaaagcaatgctgccattgctgttaagccccctgtgatgcctgcagccaatgctacccaagcagcagtctctcctgtgctcacaagatcgctctcattgcagagcacttctgtagcaactggacaacctcaagttgccgctgaccctagctcaatttgcaagctccaggctggtaagactacaagatctttttgtgaatctaaaatggggctgttttaatccctcgtcatatgatgtactaagtgattgaagatgctacaaatttattctttatttgctgtgcagatctccccattgccaggaggcactcccttcagcgcttcctagagaaacgccgtgacaggttggcatgaatgaatgtagaaatagcattctatatttgtctgatgcatttccttaggtttagtggtccattagaacatgctaaaagaatgtggatagctggaaactgacttgtaggggataaaacatttgctttagcttgacttggatacgaaccaagtatatgtgcaaggactgttttcagtttcattaccatagcccatgttttcatgatacataacaaagtagccagtagttatagaaacacagctttctgcaaatttgagaaatccttagcttcaactgattaagccaaatgaccatgttaagcaggttggtgagcaaagctccatatcccacaaaatcctccgagggcatggaagcatcagggatggaagtaactgctgagggcaaggcccagtaactgtcaacttcgaatggtggaggctgacgtttggagggggaaggaaggaaaggcgtcgcgttcattgtttagatggtgtcgcaccctagaaccttactaataatcgaacctatgcccttattatcgttgtactgttccttatgtgctagtctattctgtcatgaatttgtttactcgaacagcaaactgctatgatagtagtatatcattgtacatcatgcatatcattgcctgacaatgttatcgtgttcgtctagtaatgatatcatccaggttaattgttactagt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001060638.1 RefSeq:Os04g0653000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 4]]
[[Category:Chromosome 4]]

Os04g0653000

2014-06-04T08:05:10Z

Suchao13: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Please input function information here.

* EG2(extra glume 2)is a JA signalling repressor that interacts with a putative JA receptor, OsCOI1b, to trigger OsJAZ1’s degradation during spikelet development[1].

* Its high sequence similarity with the Arabidopsis JAZ proteins so also called OsJAZ1[1].

*It belong to TIFY family so also called TIFY family[2].

===Expression===
Please input expression information here.

EG2 expressed ubiquitously in the plant, from roots, stems and leaves, to spikelets and seeds.
[[File:eg-1.jpg]]

===Mutation and Phenotype===
Please input information here.

The EG2 gene was mapped to chromosome 4 between CQQ2 and CQQ3, two In-Del markers that define a 2.2 cM.

[[File:eg-2.jpg]]

The mutation of EG2 is eg2-1D, which caused by C-to-G transition, resulting in a substitution of Ala by Gly in the fifth amino acid of the Jas domain. This motif was known to be critical for JAZ’s interaction with the JA receptor COI1.

[[File:eg-3.jpg]]

mutants displayed defects in spikelet development,including extra glume-like structures between the sterile lemma and lemma and altered floral organ numbers and identities.

[[File:eg-4.jpg]][[File:eg-5.jpg]]

===EG2 is a repressor of JA response===
Please input information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os04g0653000|
Description = The start codon is not identified.|
Version = NM_001060638.1 GI:115461005 GeneID:4337240|
Length = 3445 bp|
Definition = Oryza sativa Japonica Group Os04g0653000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|
AP = Chromosome 4:33705210..33708654|
CDS = 33705211..33705317,33707373..33707475,33707567..33707792,33707906..33707963,33708275..33708365 |
GCID = <gbrowseImage1>
name=NC_008397:33705210..33708654
source=RiceChromosome04
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008397:33705210..33708654
source=RiceChromosome04
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>ggagaggagaggaaggaggaagaggtggtggtggaggagaagagccatcagcagcagcagcagcaaggggaggaggagctcgtcggcctctcgctcgccggcggcaggcccaaagtatttccaatgtcgagccctccacctaatccttcgcaacttacaattttctatggtggatcagtatgtgtttatgactcagtgccgccagagaaggctcaagcaatcatgcttattgccgcagcagctgctgcagcggcgtctgccaccaaaagcaatgctgccattgctgttaagccccctgtgatgcctgcagccaatgctacccaagcagcagtctctcctgtgctcacaagatcgctctcattgcagagcacttctgtagcaactggacaacctcaagttgccgctgaccctagctcaatttgcaagctccaggctgatctccccattgccaggaggcactcccttcagcgcttcctagagaaacgccgtgacaggttggtgagcaaagctccatatcccacaaaatcctccgagggcatggaagcatcagggatggaagtaactgctgagggcaaggcccagtaa</cdnaseq>|
AA = <aaseq>GEERKEEEVVVEEKSHQQQQQQGEEELVGLSLAGGRPKVFPMSS PPPNPSQLTIFYGGSVCVYDSVPPEKAQAIMLIAAAAAAAASATKSNAAIAVKPPVMP AANATQAAVSPVLTRSLSLQSTSVATGQPQVAADPSSICKLQADLPIARRHSLQRFLE KRRDRLVSKAPYPTKSSEGMEASGMEVTAEGKAQ</aaseq>|
DNA = <dnaseqindica>2..108#2164..2266#2358..2583#2697..2754#3066..3156#gggagaggagaggaaggaggaagaggtggtggtggaggagaagagccatcagcagcagcagcagcaaggggaggaggagctcgtcggcctctcgctcgccggcggcaggtagttttccggctccgccttctcctctcgcaagtcgagtcctattcagctttgggttgatcgattgggtcgcgggagagagagtatttggttggttgttcgggaaatgcatctcgcgcgggattctagccgcttttcagttcatagtatcgccccttctccgagaactcgccgccggggagccgcttcctgctgggagccgagcggcgccgcagccgcctccgaagtggtttaccggaggggatggattttctccggtactggactcctggtgtttgaatttttgggtggaaccggagactctttttttggtcgggtgaaattccttggaccgaccaaaaaaatctcgaatccactcggagtttcctccgttccagatggagctcttgcgttgaaaattttcctaaaaatagagtcaaaatgtttcgacttcgagttaagagtccaccctacaccgagttgcaactttcccttttttacgagttggtgtaatcatggaagagcccttagctttgctgcagtggatcaaattggtgggggttcttgcctaggtcatgtgaagattcttctgattagtggtagtctcatgcttctcctcggcctgcaatccaaatcccctttattcctttcccgctctcttgcgcttggcgtcagctgttcttaaccttgaacaaattagggtgtcattctagttgcacgtgttcttatccagatgttgggagtgcggtgaactgctgatattctactaaaccgatatcaattatatggatgttcaatttggttcacgtcactaggtaaatgatttcccaccttagaccaaacttaacggataggaaagaacttttggcaagtggcagatccaggtagtttttttccttccctttttctgtaattggcagttacttgttctccaaatacttggaactgctgtgaaaagtttccaattgagccttggaatcaggagcaatggggaactcttctaagttacttgcatttcttctttaaagactcagaaataataagcaagaaacacacatagatctggtgaaacttgagttagcgtgcagtttttaacacctccatgtaccaaggggattcaatggtaaaggcctcatccccttgtcttatctttacttcaagactaaatctgtagctggggaccaagtaccatagtttcgcaactcttatataatccactaggctgctgtgaaaacaatagatgcagtgtttgttttgaagtaagtaaggtgaaagtaaaaatacctggtaatggctaatatgaatcgtggctattattaaatgtttcagcattttctgaataggtaaaccacatatatgtaactatgtaagatgtttttaaatctagatgttctattgagcctgtaacctggtcttttagactagattatgctgagactttgaagaaaaaaatttcagtacattccgtccatcttggacaacctttggacagattattaactctgaatcagtcccatcatgctgcacatgttggatatttcagagtggaccataaagttgcaggcctagtttgctatttttagattttagatcttgtgagttttaatcttgatcatgctattagactcagaatgtgtgtaccaaaaacattgataatggtatctatcctgacttgggtataacttgtgttactggcagctgtgggagggctatgttggcgtagcgtactgaccaagagataatcctctcatcaagtagaatatagagagtagaataattatttatgaaggagatgggcctttatctggctgcagacaaggtgcatagtaaagctcattaaacctctaaaaaaggctggaaatacggcctacggtgtaattgcacattatacatcagttagctaggttggcctccaattttatatggcactttacccttggatgcccatcttatgtattgccatgtactcatgtatgcaattagtcaagaacggcccacttatgccaatatctgtgtaactatgcaatctgttattaccttcttgcacatttactaatttcgctttgtcataatcaggcccaaagtatttccaatgtcgagccctccacctaatccttcgcaacttacaattttctatggtggatcagtatgtgtttatgactcagtgccgccagagaaggtcatattcaccccacttatcttcgttatatcctccgactaactgttgaaagtttatgctaattcgttatctgcaacattgtcatcaacaggctcaagcaatcatgcttattgccgcagcagctgctgcagcggcgtctgccaccaaaagcaatgctgccattgctgttaagccccctgtgatgcctgcagccaatgctacccaagcagcagtctctcctgtgctcacaagatcgctctcattgcagagcacttctgtagcaactggacaacctcaagttgccgctgaccctagctcaatttgcaagctccaggctggtaagactacaagatctttttgtgaatctaaaatggggctgttttaatccctcgtcatatgatgtactaagtgattgaagatgctacaaatttattctttatttgctgtgcagatctccccattgccaggaggcactcccttcagcgcttcctagagaaacgccgtgacaggttggcatgaatgaatgtagaaatagcattctatatttgtctgatgcatttccttaggtttagtggtccattagaacatgctaaaagaatgtggatagctggaaactgacttgtaggggataaaacatttgctttagcttgacttggatacgaaccaagtatatgtgcaaggactgttttcagtttcattaccatagcccatgttttcatgatacataacaaagtagccagtagttatagaaacacagctttctgcaaatttgagaaatccttagcttcaactgattaagccaaatgaccatgttaagcaggttggtgagcaaagctccatatcccacaaaatcctccgagggcatggaagcatcagggatggaagtaactgctgagggcaaggcccagtaactgtcaacttcgaatggtggaggctgacgtttggagggggaaggaaggaaaggcgtcgcgttcattgtttagatggtgtcgcaccctagaaccttactaataatcgaacctatgcccttattatcgttgtactgttccttatgtgctagtctattctgtcatgaatttgtttactcgaacagcaaactgctatgatagtagtatatcattgtacatcatgcatatcattgcctgacaatgttatcgtgttcgtctagtaatgatatcatccaggttaattgttactagt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001060638.1 RefSeq:Os04g0653000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 4]]
[[Category:Chromosome 4]]

File:Eg-5.jpg

2014-06-04T08:03:10Z

Suchao13:

File:Eg-4.jpg

2014-06-04T08:02:52Z

Suchao13: