http://192.168.164.12:81/ricewiki/api.php?action=feedcontributions&feedformat=atom&user=Victor RiceWiki - User contributions [en] 2026-05-23T13:16:09Z User contributions MediaWiki 1.30.0 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=174041 Os09g0327600 2014-05-29T12:45:26Z <p>Victor: </p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot; /&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> The rice blast resistance gene was identified by analyzing 281 recombinant inbred lines (RILs) of an F7 recombinant inbred population of the Moroberekan/CO39 cross with 127 restriction fragment length polymorphism (RFLP) markers&lt;ref name=&quot;ref2&quot; /&gt;.<br /> ===Expression===<br /> ===Evolution===<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to ''Magnaporthe oryzae'' requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Wang G L, Mackill D J, Bonman J M, et al. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar[J]. Genetics, 1994, 136(4): 1421-1434.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = ''Magnaporthe grisea resistance-5''(pi5-1)|<br /> Description = rice blast resistance gene|<br /> Version = GI:213958601 GeneID:4346749|<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> AP = Chromosome 9:9,619,375..9,621,822|<br /> CDS = Chromosome 9:5278..5592,6568..7716,7915..9231,9672..9831,<br /> 10693..10829|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atg <br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga ag<br /> ggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcag<br /> aatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga g<br /> ccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca g<br /> ctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatga<br /> <br /> <br /> <br /> <br /> &lt;/cdnaseq&gt;|<br /> <br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=174038 Os09g0327600 2014-05-29T12:28:55Z <p>Victor: </p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot; /&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> The rice blast resistance gene was identified by analyzing 281 recombinant inbred lines (RILs) of an F7 recombinant inbred population of the Moroberekan/CO39 cross with 127 restriction fragment length polymorphism (RFLP) markers&lt;ref name=&quot;ref2&quot; /&gt;.<br /> ===Expression===<br /> ===Evolution===<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to ''Magnaporthe oryzae'' requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Wang G L, Mackill D J, Bonman J M, et al. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar[J]. Genetics, 1994, 136(4): 1421-1434.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene|<br /> Version = GI:213958601 GeneID:4346749|<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> AP = Chromosome 9:9,619,375..9,621,822|<br /> CDS = Chromosome 9:5278..5592,6568..7716,7915..9231,9672..9831,<br /> 10693..10829|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atg <br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga ag<br /> ggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcag<br /> aatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga g<br /> ccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca g<br /> ctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatga<br /> <br /> <br /> <br /> <br /> &lt;/cdnaseq&gt;|<br /> <br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=174037 Os09g0327600 2014-05-29T12:26:48Z <p>Victor: </p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot; /&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> The rice blast resistance gene was identified by analyzing 281 recombinant inbred lines (RILs) of an F7 recombinant inbred population of the Moroberekan/CO39 cross with 127 restriction fragment length polymorphism (RFLP) markers&lt;ref name=&quot;ref2&quot; /&gt;.<br /> ===Expression===<br /> ===Evolution===<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to ''Magnaporthe oryzae'' requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Wang G L, Mackill D J, Bonman J M, et al. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar[J]. Genetics, 1994, 136(4): 1421-1434.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene|<br /> Version = GeneID:4346749|<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> AP = Chromosome 9:9,619,375..9,621,822|<br /> CDS = Chromosome 9:5278..5592,6568..7716,7915..9231,9672..9831,<br /> 10693..10829|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atg <br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga ag<br /> ggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcag<br /> aatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga g<br /> ccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca g<br /> ctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatga<br /> <br /> <br /> <br /> <br /> &lt;/cdnaseq&gt;|<br /> <br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=174036 Os09g0327600 2014-05-29T12:17:35Z <p>Victor: </p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot; /&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> The rice blast resistance gene was identified by analyzing 281 recombinant inbred lines (RILs) of an F7 recombinant inbred population of the Moroberekan/CO39 cross with 127 restriction fragment length polymorphism (RFLP) markers&lt;ref name=&quot;ref2&quot; /&gt;.<br /> ===Expression===<br /> ===Evolution===<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to ''Magnaporthe oryzae'' requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Wang G L, Mackill D J, Bonman J M, et al. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar[J]. Genetics, 1994, 136(4): 1421-1434.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene|<br /> Version = GeneID:4346749|<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> AP = Chromosome 9:9,619,375..9,621,822|<br /> CDS = Chromosome 9:5278..5592,6568..7716,7915..9231,9672..9831,<br /> 10693..10829|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> <br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=174035 Os09g0327600 2014-05-29T12:16:19Z <p>Victor: </p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot; /&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> The rice blast resistance gene was identified by analyzing 281 recombinant inbred lines (RILs) of an F7 recombinant inbred population of the Moroberekan/CO39 cross with 127 restriction fragment length polymorphism (RFLP) markers&lt;ref name=&quot;ref2&quot; /&gt;.<br /> ===Expression===<br /> ===Evolution===<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to ''Magnaporthe oryzae'' requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Wang G L, Mackill D J, Bonman J M, et al. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar[J]. Genetics, 1994, 136(4): 1421-1434.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene|<br /> Version = GeneID:4346749|<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> AP = Chromosome 9:9,619,375..9,621,822|<br /> CDS = Chromosome 9:5278..5592,6568..7716,7915..9231,9672..9831|<br /> 10693..10829<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> <br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=174034 Os09g0327600 2014-05-29T12:07:44Z <p>Victor: </p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot; /&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> The rice blast resistance gene was identified by analyzing 281 recombinant inbred lines (RILs) of an F7 recombinant inbred population of the Moroberekan/CO39 cross with 127 restriction fragment length polymorphism (RFLP) markers&lt;ref name=&quot;ref2&quot; /&gt;.<br /> ===Expression===<br /> ===Evolution===<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to ''Magnaporthe oryzae'' requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Wang G L, Mackill D J, Bonman J M, et al. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar[J]. Genetics, 1994, 136(4): 1421-1434.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene|<br /> Version = GeneID:4346749|<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> AP = Chromosome 9:9,619,375..9,621,822|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> <br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=174033 Os09g0327600 2014-05-29T12:04:05Z <p>Victor: </p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot; /&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> The rice blast resistance gene was identified by analyzing 281 recombinant inbred lines (RILs) of an F7 recombinant inbred population of the Moroberekan/CO39 cross with 127 restriction fragment length polymorphism (RFLP) markers&lt;ref name=&quot;ref2&quot; /&gt;.<br /> ===Expression===<br /> ===Evolution===<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to ''Magnaporthe oryzae'' requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Wang G L, Mackill D J, Bonman J M, et al. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar[J]. Genetics, 1994, 136(4): 1421-1434.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene|<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> AP = Chromosome 9:9,619,375..9,621,822|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008402.1:9619375..9621822<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> <br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=174032 Os09g0327600 2014-05-29T11:47:53Z <p>Victor: </p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot; /&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> The rice blast resistance gene was identified by analyzing 281 recombinant inbred lines (RILs) of an F7 recombinant inbred population of the Moroberekan/CO39 cross with 127 restriction fragment length polymorphism (RFLP) markers&lt;ref name=&quot;ref2&quot; /&gt;.<br /> ===Expression===<br /> ===Evolution===<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to ''Magnaporthe oryzae'' requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Wang G L, Mackill D J, Bonman J M, et al. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar[J]. Genetics, 1994, 136(4): 1421-1434.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene|<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> AP = Chromosome 9:9,619,375..9,621,822|<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:19856181..19857859<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=174010 Os09g0327600 2014-05-29T09:29:07Z <p>Victor: </p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot; /&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> The rice blast resistance gene was identified by analyzing 281 recombinant inbred lines (RILs) of an F7 recombinant inbred population of the Moroberekan/CO39 cross with 127 restriction fragment length polymorphism (RFLP) markers&lt;ref name=&quot;ref2&quot; /&gt;.<br /> ===Expression===<br /> ===Evolution===<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to ''Magnaporthe oryzae'' requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Wang G L, Mackill D J, Bonman J M, et al. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar[J]. Genetics, 1994, 136(4): 1421-1434.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> AP = Chromosome 9:9,619,375..9,621,822|<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:19856181..19857859<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=174004 Os09g0327600 2014-05-29T09:21:01Z <p>Victor: </p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot; /&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> The rice blast resistance gene was identified by analyzing 281 recombinant inbred lines (RILs) of an F7 recombinant inbred population of the Moroberekan/CO39 cross with 127 restriction fragment length polymorphism (RFLP) markers&lt;ref name=&quot;ref2&quot; /&gt;.<br /> ===Expression===<br /> ===Evolution===<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to ''Magnaporthe oryzae'' requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.&lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Wang G L, Mackill D J, Bonman J M, et al. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar[J]. Genetics, 1994, 136(4): 1421-1434.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:19856181..19857859<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=174002 Os09g0327600 2014-05-29T09:19:05Z <p>Victor: </p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot; /&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> The rice blast resistance gene was identified by analyzing 281 recombinant inbred lines (RILs) of an F7 recombinant inbred population of the Moroberekan/CO39 cross with 127 restriction fragment length polymorphism (RFLP) markers&lt;ref name=&quot;ref2&quot; /&gt;.<br /> ===Expression===<br /> ===Evolution===<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to ''Magnaporthe oryzae'' requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.&lt;/ref&gt;<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Wang G L, Mackill D J, Bonman J M, et al. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar[J]. Genetics, 1994, 136(4): 1421-1434.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:19856181..19857859<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=173996 Os09g0327600 2014-05-29T09:12:35Z <p>Victor: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot; /&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> The rice blast resistance gene was identified by analyzing 281 recombinant inbred lines (RILs) of an F7 recombinant inbred population of the Moroberekan/CO39 cross with 127 restriction fragment length polymorphism (RFLP) markers&lt;ref name=&quot;ref2&quot; /&gt;.<br /> ===Expression===<br /> ===Evolution===<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to ''Magnaporthe oryzae'' requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.&lt;/ref&gt;<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;Wang G L, Mackill D J, Bonman J M, et al. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar[J]. Genetics, 1994, 136(4): 1421-1434.&lt;/ref&gt;<br /> &lt;references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:19856181..19857859<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=173992 Os09g0327600 2014-05-29T09:10:51Z <p>Victor: </p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot; /&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> The rice blast resistance gene was identified by analyzing 281 recombinant inbred lines (RILs) of an F7 recombinant inbred population of the Moroberekan/CO39 cross with 127 restriction fragment length polymorphism (RFLP) markers&lt;ref name=&quot;ref2&quot; /&gt;.<br /> ===Expression===<br /> ===Evolution===<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to ''Magnaporthe oryzae'' requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.&lt;/ref&gt;<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;<br /> Wang G L, Mackill D J, Bonman J M, et al. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar[J]. Genetics, 1994, 136(4): 1421-1434.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:19856181..19857859<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=173984 Os09g0327600 2014-05-29T09:04:47Z <p>Victor: </p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot; /&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> identified by analyzing 281 recombinant inbred lines (RILs) of an F7 recombinant inbred population of the Moroberekan/CO39 cross with 127 restriction fragment<br /> length polymorphism (RFLP) markers&lt;ref name=&quot;ref2&quot; /&gt;.<br /> ===Expression===<br /> ===Evolution===<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to ''Magnaporthe oryzae'' requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.&lt;/ref&gt;<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;<br /> Wang G L, Mackill D J, Bonman J M, et al. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar[J]. Genetics, 1994, 136(4): 1421-1434.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:19856181..19857859<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=173972 Os09g0327600 2014-05-29T08:56:49Z <p>Victor: </p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot; /&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> ===Expression===<br /> ===Evolution===<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.&lt;/ref&gt;<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;<br /> Yi G, Lee S K, Hong Y K, et al. Use of Pi5 (t) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea[J]. Theoretical and applied genetics, 2004, 109(5): 978-985.&lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:19856181..19857859<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=173964 Os09g0327600 2014-05-29T08:44:00Z <p>Victor: </p> <hr /> <div>==Introduction==<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot;&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> ===Expression===<br /> ===Evolution===<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;<br /> Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.<br /> &lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;<br /> Yi G, Lee S K, Hong Y K, et al. Use of Pi5 (t) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea[J]. Theoretical and applied genetics, 2004, 109(5): 978-985.<br /> &lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:19856181..19857859<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=173959 Os09g0327600 2014-05-29T08:36:02Z <p>Victor: </p> <hr /> <div>==Introduction==<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot;&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> ==Expression==<br /> ==Evolution==<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;<br /> Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.<br /> &lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;<br /> Yi G, Lee S K, Hong Y K, et al. Use of Pi5 (t) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea[J]. Theoretical and applied genetics, 2004, 109(5): 978-985.<br /> &lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:19856181..19857859<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=173953 Os09g0327600 2014-05-29T08:31:43Z <p>Victor: </p> <hr /> <div>==Introduction==<br /> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice&lt;ref name=&quot;ref1&quot;&gt;. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> ===Expression===<br /> ===Evolution===<br /> ==Labs working on this gene==<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;<br /> Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.<br /> &lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;<br /> Yi G, Lee S K, Hong Y K, et al. Use of Pi5 (t) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea[J]. Theoretical and applied genetics, 2004, 109(5): 978-985.<br /> &lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:19856181..19857859<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=173949 Os09g0327600 2014-05-29T08:29:16Z <p>Victor: /* References */</p> <hr /> <div> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;<br /> Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.<br /> &lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;<br /> Yi G, Lee S K, Hong Y K, et al. Use of Pi5 (t) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea[J]. Theoretical and applied genetics, 2004, 109(5): 978-985.<br /> &lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:19856181..19857859<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=173110 Os09g0327600 2014-05-27T02:56:16Z <p>Victor: </p> <hr /> <div> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;<br /> S.Fukuoka;K.Okuno QTL analysis and mapping of pi21, a recessive gene for field resistance to rice blast in Japanese upland rice Theoretical and Applied Genetics, 2001, 103(2-3): 185-190<br /> &lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;<br /> Shuichi Fukuoka;Norikuni Saka;Hironori Koga;Kazuko Ono;Takehiko Shimizu;Kaworu Ebana;Nagao Hayashi;Akira Takahashi;Hirohiko Hirochika;Kazutoshi Okuno;Masahiro Yano Loss of Function of a Proline-Containing Protein Confers Durable Disease Resistance in Rice Science, 2009, 325(5943): 998-1001<br /> &lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:19856181..19857859<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# 1 aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> 61 ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> 121 gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> 181 ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> 241 gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> 301 cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> 361 catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> 421 tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> 481 acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> 541 ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> 601 ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> 661 ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> 721 tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> 781 tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> 841 cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> 901 atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> 961 agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> 1021 cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> 1081 cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> 1141 actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/213958600]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 9]]<br /> [[Category:Chromosome 9]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=173107 Os09g0327600 2014-05-27T02:49:55Z <p>Victor: </p> <hr /> <div> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;<br /> S.Fukuoka;K.Okuno QTL analysis and mapping of pi21, a recessive gene for field resistance to rice blast in Japanese upland rice Theoretical and Applied Genetics, 2001, 103(2-3): 185-190<br /> &lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;<br /> Shuichi Fukuoka;Norikuni Saka;Hironori Koga;Kazuko Ono;Takehiko Shimizu;Kaworu Ebana;Nagao Hayashi;Akira Takahashi;Hirohiko Hirochika;Kazutoshi Okuno;Masahiro Yano Loss of Function of a Proline-Containing Protein Confers Durable Disease Resistance in Rice Science, 2009, 325(5943): 998-1001<br /> &lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os09g0327600|<br /> Description = rice blast resistance gene<br /> Length = 11516bp|<br /> Definition = Oryza sativa Japonica Group Pi5-1 (Pi5-1) gene, complete cds.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:19856181..19857859<br /> source=RiceChromosome09<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> AA = &lt;aaseq&gt;MVGAEMLVAAAVSQVARKINDIVGVAQGEVKLCCNFSDDLEGIK<br /> DTLVYLETLLKNAENNSFGSDRANLRHWLGQIKSLAYDIEDIVDGYYSSKEQFDGGSY<br /> AQKGSLFCSLSNPMLLKGSMVYKMKSKREMLQQSQQLPNQYHFLSYINSAVHYFEEKQ<br /> TTSYRNTDIAIVGRDADLDHLMDLLMQNSAEELCIIPIVGPVGFGKTSLAQLVFNDTR<br /> TEVFSFRIWVHVSMGNINLEKIGRDIVSQTTEKIEGNMQLQSIKNAVQRVLNKYSCLI<br /> IIDSLWGKDEEVNELKQMLLTGRHTESKIIVTTHSNKVAKLISTVPLYKLAALSEDDC<br /> LKIFSQRAMTGPGDPLFREYGEEIVRRCEGTPLVANFLGSVVNAQRQRREIWQAAKDE<br /> EMWKIEEDYPQDKISPLFPSFKIIYYNMPHELRLCFVYCSIFPKGTVIEKKKLIQQWI<br /> ALDMIESKHGTLPLDVTAEKYIDELKAIYFLQVLERSQNDAERSSASEEMLRMHNLAH<br /> DLARSVAGEDILVILDAENERNARYCNYRYAQVSASSLESIDRKAWPSKARSLIFKNS<br /> GVDFEHVSEVLSVNKYLRVLDLSGCCVQDIPSPIFQLKQLRYLDVSSLSITALPLQIS<br /> SFHKLQMLDLSETELTELPPFISNLKGLNYLNLQGCQKLQRLNSLHLLHDLHYLNLSC<br /> CPEVTSFPESIENLTKLRFLNLSGCSKLSTLPIRFLESFASLCSLVDLNLSGFEFQML<br /> PDFFGNIYSLQYLNLSKCLKLEVLPQSFGQLAYLKSLNLSYCSDLKLLESFECLTSLR<br /> FLNLSNCSRLEYLPSCFDKLNNLESLNLSQCLGLKALPESLQNLKNLQLDVSGCQDCI<br /> VQSFSLSTRSSQSCQRSEKAEQVRSRNSEISEITYEEPAEIELLKNNPSKDLASISHL<br /> NEDRIEEPEVVTEPSATRGMVQQIPGNQLSSPSSHLSSFASSSAPFASSSSDTSTSEH<br /> PVPNEEAAALTVPRSKEKCDNTPMPVKDGLISEDDAPVHLHQKPLQATAMAAI&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531# aagcttgaaa gtgtgtgaag aaatgaagaa agtgaagtct acttatagga caggtataac<br /> ggttatgaga agtggaaatg tccgaactgc cccgcaaccg ccatcaggga gtgatctgga<br /> gcgtccacgc caaaccccag gatcaacggt gatcaccagg gttcggctga acctgggctg<br /> ggccagatgg cttccaaacc tagtctgggt ctcctcctcg acctgcctta tccaattctt<br /> gcaattttgg gatgtttctt tagtagttta aaagcccatt cttgcattga tagatttctc<br /> cttcactttt agtctggatt tcctcccaac ttctgggttt atcacctgca taaaaatata<br /> catcaacaat tgtggaatat gtgagtttta acacctatca ctatgttgga tattttatta<br /> tctggacttt atgcagacgt tgacggtata gatttggcat ttaacagtcg ccaacattat<br /> acaatcgggt aaatagtatt gatgtattcc atgaatctgt gtatagaagg tatgccatat<br /> ccttgacact gacaccatat gtgtttccgt ttttgagaaa acatttccat ttccaaaaat<br /> ttctgaccaa cactctctat ttccgaaaac tcatctgaaa tccgaaaact tttcaaaccg<br /> ttttcatccc agtaatgaat ggcaggctct acgtgcagac ccacaaatgc tccacaaaga<br /> tctcaaaaaa tttatcgtat aaatttcata cgtagactaa atttcacaaa aattcacttg<br /> tgtacccatt gaggtatgta ttttgttttc aaaaaaaaac acagtttacc ttgtggtgct<br /> cgggacatcg gccggagctc atgatcataa tggagtcagt cagggtggct actggagctt<br /> atggcggagg aggtcagggt ggtgggacgt ggggcagagt ttagtccggt cggcaaccag<br /> agctccgaga ggagatctgg gcagtagtga gagaagggtc aggaggcgcc agagtggcca<br /> cgacggaggc tgggaaagcg atgccgaaca gcaaagcatg cgttgaagta ggcggggtgg<br /> cagcagcctc ggggcggagc gggcatggaa caagggtaga gctggagtcg gtggccggct<br /> actgagacca ttcacagtgc gtcaccaagt tttgatattc ttcacatgcc acgtagatca<br /> 1201 gagcggtatt agccacatgt tacatagtgc aaaactctac aagaccacat atgggttgct<br /> 1261 tgtagtgggt cccactaata ccacttttac acttctcttt ttacttttcc cctctcctct<br /> 1321 ctcccctctc ttcctttccc caccggccac cgcagagagc cgggcggaga cggaggttgc<br /> 1381 gggggtcggg cggagcggcg gctcaagcgg aggtcgtgga ggcgagcagg agacggcgcc<br /> 1441 gggccgagtg gcgacggcgg tggggcctag caccgtgcct cccctcctcc tcctcctcta<br /> 1501 gtggtggcga atcgaggaag ccgccgccgc cgcgccccac ctcttcatct gatgcgggcg<br /> 1561 ggaagctgcc gccaccgccg tcgggcagat ccagatctcc ggcctccgtg tcgggcggct<br /> 1621 acgcggcggc gggcgtcggc gtcggtcagc gccctcctca cctggccagc ccaccgacta<br /> 1681 gccctaccgt cgtcatcatc cctggtcagc accctcctcc cctggcattc ggctgcctca<br /> 1741 cccctctcct ctctctcctc tagcggtggt gtgggtccca cctgtgggtc cctctgagac<br /> 1801 gaagaacgcg tagtcaactt tggctacgcg cggagctgag gaacgcacac gattggataa<br /> 1861 agtggcaaag gaatatttgc ttttttggtt gaggctaaat gctacgtaga tctactgtga<br /> 1921 atggcctgat ggcgcagcgg ctctcttgcg ggacagagtg ggacgtgcac gacgagaaat<br /> 1981 ttcctcttgc atgaataacc cacggatgta ggcctcctta actaggagct acgaggtggg<br /> 2041 atttgggagg ctttttctat ttcaaggctt aaagcctatc caatatgatc tctaatctta<br /> 2101 aaatttagtg tttatttttg aaaaaaagtt ggctccaata attttcctaa ttagcttcca<br /> 2161 aaaattggaa tcttactccc tatccttctc gctctccact agtggagaag cgtgctagtt<br /> 2221 cttaacacac gcatgggtgg agggatcaca tggatattat ggaaatatcc tcatggactg<br /> 2281 gtgattaaca tagtcctact aggagagaat tgagtttatt ctatatcgtt atcgacgttt<br /> 2341 ggtatcacga ctacgttatt tggatggtat ggggatcatc ggtactagag tatatgcgag<br /> 2401 attgaggtaa aagagatgga gacagatatt tttatactgg ttcgcccctt atctaacagg<br /> 2461 taatagccct acatcctgtt ggctaaagcc ggtattgctc ttattcatct gaatcgcaca<br /> 2521 agtataatat ttaggataac ctgtctagct gtcatcgact tggcggcatg gataaccaac<br /> 2581 tcgtagtcga tgacggggta gtatttctct tcgaatatga actcgtcgag atcagagatg<br /> 2641 gctctagatc tctcttgccg gtctcaggag gcaccagatg gggtatgcct aggctaatct<br /> 2701 ctaatgtcga tatttagcgg cgtattggct tgtgtgtatg ttatgtgttg tggctcgtca<br /> 2761 tctctcctcc tagggggctt gtatttatac ccatagatgc ccccttgtct aagtagaact<br /> 2821 agggagataa atatggatac gatccgagta gtccttgtcg tttccatata gaactctttt<br /> 2881 tgtcctttct tatccgaaac tccttttata tacgaggtat gattccatat aagacatggt<br /> 2941 atatggtggg ccctgccgag cttagtcagg aatgtggtat ccacaaccct gacaatcatg<br /> 3001 cagggttcat tgtctccgag ttctacgcgg agactaagac aactcaaggt ataaaaggcg<br /> 3061 cactctctaa ggggttcaaa agagcaaatc atagcacgaa cacacccacc atagtttacg<br /> 3121 aagccagagg ctgtgaagcc tactcgccag gagatcttgt cgactcatct cgacaaggat<br /> 3181 ctcgccggta acgctggatt catctcttct ctttgtactc cgtggtttcc atatcaatct<br /> 3241 catataaact ggattatggt tattatctta cgaggggtct aaaccagtat aatctttgtc<br /> 3301 tctctgattg ttttaatatc gtatcatgta gatcctcata ccaacttacc ctaatacact<br /> 3361 atttatccga tctacaggta tcccctatcg acaataggta tataagatat tataaaaaag<br /> 3421 atggagacat atgtgagctg atgatttagg ctgcattcgt tgcagcttct ttccaaccca<br /> 3481 tctccctcgt tttccgtgcg catgcttttc aaactgctaa acggtgcgtt ttttacaaaa<br /> 3541 agtttatata cgaaagttgc ttaaaaaaat catattgatc cattttttaa aaagatagca<br /> 3601 aaaaattaag taatcacacg ctaatggact actctgtttt ccgtgccgga ggatagcttt<br /> 3661 cccaacccag ggaaacgaac ccaaccttag gtgtgttcat tgctaggtgt tcccaacccc<br /> 3721 tctcccttat attccgtgcg catgcttttc aaactgttaa acggtgtgtc tctttttaaa<br /> 3781 aaatttctat acgaaagttg ctaaaaaaat catatcaata catttttgaa aaaaaaagct<br /> 3841 aatacttaat taatcacgta cgtactaata gaccgcttcg ttttctatgc gcagaagatt<br /> 3901 tgttcccaac ccccacaaca aacacagcct taacatgctt gcatttaaaa agctataaaa<br /> 3961 tttaataaat tataaaatta tagataatat aacatgctta cttgatgtga cactttacct<br /> 4021 gttaagtttt aggagtcggt gtttagattc aaactttttt tttcaaactt ccaacttttc<br /> 4081 catcagatca aatgtttggg cacatgcatt cagcaataaa tgtggacaaa aaaaaaccag<br /> 4141 ttgtacagtt tgcatgtaaa tcacgagatg aatcttttga gcctaattac gtcatgattt<br /> 4201 gacaatgtgg tgctaaagta aatatttact aatgacagat taattaggtt taatagattt<br /> 4261 gtctcgcagt ttacaggcga aatatataat tttttttgtt attagtttat atttaatact<br /> 4321 taaaatgtat gtccatatac ttaaaaaaat tttgtaccac gaactaaaca cagccaagtg<br /> 4381 gactctaact ctctctctct ctatatatat atatatatat atatagtaat gtgttcgtat<br /> 4441 gtcctggata gaaactcatt tcctccgcat agaaaacgga gcggtctatt aatacgtgat<br /> 4501 taattaagta ttagctattt ttttcgaaaa taaattaatt taatttttta aataacattt<br /> 4561 atatagaaac tttttaaaaa acacgttgat taaccatttg aaaagcgtgc gtgcacgcgg<br /> 4621 cgtgaaaaat gaggcagaga tgttgtgaaa aggagtgccg aacacagtca aagcctcagg<br /> 4681 tggtgtttgg atccagggac ttaacttaaa ctttagtccc tatatttaga cactaattta<br /> 4741 tagtattaaa tatagactac ttataaaact aattacataa atgaaagcta attcacgaga<br /> 4801 caattttttt aagcctaatt aatccataat taaagaatgt ttactgtagc atcacatagg<br /> 4861 ctaatcatgg attaattaga cttaatagat tcatctcgtg aattagtcca agattataga<br /> 4921 tgggctttat taatagtcta cgtttaatat ttataattaa ttttcaatcc aatatgatag<br /> 4981 gacttaaaat ttagtcccat ctacagggtc agaggattcg gtcggtctca gggcagtcct<br /> 5041 ctccgtataa cgcagcgccc gatatttttt atgggcataa atagtctgat tgctactgta<br /> 5101 gcattttgtt caactaactc cccaatgggc aatggctagt cgtcgagtgt cagtagtcaa<br /> 5161 gagtagactc cgcttcgctt cgccgtacct tctctcttct cgttctccta tttcacaaat<br /> 5221 cacaaccgga ttgctttctt ccttcctccg gtctggtccc caacctccgc ggcagccatg<br /> 5281 gttggcgccg agatgcttgt ggccgcggcg gtgagccagg tcgcccggaa gatcaacgac<br /> 5341 atcgtggggg tcgcgcaggg cgaggtgaag ctgtgctgca atttcagcga cgatttggag<br /> 5401 ggcatcaagg atacccttgt gtacctggaa accttgctga aaaatgcgga gaataactcc<br /> 5461 ttcggaagcg acagggccaa cctgcgccac tggcttggcc agatcaagtc cctggcttac<br /> 5521 gatatcgaag atatcgttga tgggtactac tcttccaagg agcagttcga tgggggcagc<br /> 5581 tatgcacaga aggtaacaga atctcattcc tttttcttca tcggtaaaat ttcttcaatt<br /> 5641 tcaactcaat tttagaatgc cccgcaaaaa aaaaatcaat tttagaatgg atctacatta<br /> 5701 atgagatgta gaggtgtatt actatgggca ggggaagcac cgtgtgtgca tccttagtta<br /> 5761 ctgcataggc aataagttat cctttccatt gtccaaaaaa attttgaagc aaggaggaaa<br /> 5821 agcttgaagc ttagtctttt agttttttct ttttgattat tttgttattt atctcagtta<br /> 5881 tgagattagg agtgtatatg cactcgtgta gcttttgtct gtgtgtggat atagaggctg<br /> 5941 gatttctatc cattatctta aataaattgt ccaagaaatt tatcagggga aatgcagtta<br /> 6001 gatgcaccat tagaattgct tcattgcctg tggaagagtg gaacagctct gagaattgtg<br /> 6061 attatgtttt atatatttaa acaatattga ttactagtac tagatttact ctcttttttt<br /> 6121 ttccttatga aattccttga tactggtagt agtgagagat aaacctaata attacatgcc<br /> 6181 acatacctgt agattgtact atacttcaac acccttttgc aaatagtgag agataaacct<br /> 6241 aatacttact tacatgccac atcgtcagag cactcaattc ttttttgttt tggtaagcaa<br /> 6301 tcagcttttg cctttacata gacaaaaatt gatcgacgaa tattttgaaa aaaaaaacct<br /> 6361 ttgtatttat atggaaaaga aaatgcaagg tactttacca aacaattgtg catctatgat<br /> 6421 ctatctgcta tgtaggggtt gataccggcc ttggttcctt gctgcaccaa ctgttattta<br /> 6481 cctatctcca tttttgttct tccagcaaca atagtatcaa aatactaaat gtctttcctg<br /> 6541 actaaggcta ccttcataaa tttacagggg tcattattct gctcgctatc caatccaatg<br /> 6601 cttctgaaag gtagcatggt ttataagatg aaatccaaga gagagatgct acagcaaagc<br /> 6661 caacagttgc ccaatcagta tcatttcctt tcatatatca attcagctgt gcattatttt<br /> 6721 gaggagaagc aaacaacatc atacagaaat actgacattg caattgtcgg gagggatgct<br /> 6781 gatttggatc atctcatgga tcttttaatg caaaacagcg ctgaagagct ttgtattata<br /> 6841 cccatagttg ggcctgtagg ttttggaaag acaagccttg cacagttagt tttcaatgat<br /> 6901 acaagaacag aggtattcag ctttaggata tgggttcatg tttccatggg taatatcaac<br /> 6961 cttgaaaaaa ttgggagaga tatagtttca caaactacag aaaaaattga gggaaatatg<br /> 7021 cagctgcagt caatcaagaa tgctgttcag cgtgtgctaa ataaatatag ttgcttgatc<br /> 7081 ataatagaca gcctttgggg aaaggatgaa gaagtgaatg aattgaagca gatgttgctt<br /> 7141 acaggtagac acacagaaag caagatcata gtgaccactc atagcaataa agtagctaag<br /> 7201 ctgatttcca ccgttccact gtacaagttg gcagctttat ctgaggatga ttgtttaaaa<br /> 7261 atattctctc aaagggcaat gacaggtccg ggtgacccgt tgttcaggga atatggagaa<br /> 7321 gaaatcgtta gaaggtgtga aggcacaccc ttggtagcca attttctcgg ttctgtggtg<br /> 7381 aatgctcaac gacaaaggcg tgagatttgg caagctgcaa aggatgaaga aatgtggaag<br /> 7441 atagaggaag attatcccca agacaaaatt tcaccactat ttccatcatt caagataata<br /> 7501 tattataata tgccccatga gctaagatta tgctttgtat attgttcaat cttccctaaa<br /> 7561 ggaactgtta tagaaaagaa gaaacttatt cagcaatgga ttgcacttga catgattgag<br /> 7621 tccaaacatg gaaccttgcc acttgatgta actgcggaga aatatattga tgaacttaaa<br /> 7681 gcaatctatt tccttcaagt tttagagcgg tctcaggtaa gttcatgggt tgctttttac<br /> 7741 cttctgtaca tatcctatgt aactagaatg tggttaaata tctccattaa gcatagtagc<br /> 7801 ttataccatt gttttatttc taaattctca ataagtttct gtaagaagat tgaccatgat<br /> 7861 agaatggcca atagtgatat ctcaacaaac aagtaacact gttttcctcc acagaatgat<br /> 7921 gcagaaagat ccagtgcttc tgaggaaatg cttcgcatgc ataacttggc tcatgatctt<br /> 7981 gctagatcgg ttgctggtga agatatcctt gttattttag atgccgagaa tgagcgcaat<br /> 8041 gccagatatt gcaattaccg ttatgcacag gtgtctgctt ctagtttaga gtcaatcgat<br /> 8101 cgcaaggcat ggccttccaa ggcaaggtca ctaattttca agaatagtgg tgtggacttt<br /> 8161 gagcatgtca gtgaagttct ttcagtgaac aaatacctgc gtgttttgga tctcagtgga<br /> 8221 tgttgtgttc aagatattcc atctcctatc tttcagctga aacaattgag atacctcgac<br /> 8281 gtttcatctt tatctattac agcactccct ctgcaaatta gtagctttca taagttacaa<br /> 8341 atgttggatc tttcagaaac tgaactaaca gagttgccac cctttataag caacttaaaa<br /> 8401 ggactgaatt atttgaatct ccaaggttgc cagaaacttc aacgattgaa tagccttcat<br /> 8461 ttgttgcatg atctacatta cctaaacttg tcatgctgcc ctgaagttac tagttttcct<br /> 8521 gaatctattg aaaatctgac caaactccgt ttcttgaatc tttctggatg ctctaagctt<br /> 8581 tcaacattac ctatcagatt tttggaatca tttgctagcc tctgttcttt ggtagatctt<br /> 8641 aacttaagtg gctttgaatt ccaaatgttg cccgactttt ttggcaacat atattcactt<br /> 8701 cagtatttaa atctgtcaaa atgtttgaaa cttgaggtat taccacaatc ttttggccaa<br /> 8761 cttgcatatc tgaaaagcct aaatctttca tattgttctg atcttaaact gctggaatcc<br /> 8821 tttgaatgcc ttacctctct tcggtttttg aatctctcga actgctctag gcttgaatat<br /> 8881 ttgccatcat gctttgacaa gcttaataat ttagagtctc tgaatttatc acaatgtctt<br /> 8941 ggacttaaag cactacctga atcacttcaa aaccttaaaa atcttcagct tgatgtttct<br /> 9001 gggtgtcagg attgtatagt acaatccttt tctctaagta ccagaagttc ccagtcctgc<br /> 9061 caacggtcgg agaaagctga gcaggtcaga tcaagaaaca gtgaaatttc agagatcact<br /> 9121 tatgaggaac ctgctgagat tgaactttta aagaataatc caagtaaaga tttggcctcc<br /> 9181 atctcacacc taaatgagga tagaattgag gagcctgaag ttgtcactga ggtaaactta<br /> 9241 cattttatta aagaaataaa aacaatttgc ctagtgtttc ctttgaaatt tccttatgtc<br /> 9301 aatacttaat ttatctttga tagatttgag ttactggtga ttgagaaagt tgtatgccaa<br /> 9361 tttgaaccag tttctcacta ccaactgaaa atgatgacga acggaaactt tattgtagtt<br /> 9421 gtgctcgaat tgaagatccc ttttctaatg aggtgatcta attgggtacc agaacatgaa<br /> 9481 tcatactttt ttcagtagtt cttaacattt cgtagagaaa atacatgagt gttccttcaa<br /> 9541 ttaaaaaact ccctagaggc cagcaagtta taaatttaaa tgggattctc cttattacct<br /> 9601 agatttatgt tttcagaaaa ttttgtaata tcatactaac taattgtcca tgtccttgtt<br /> 9661 tcttgatgta gccaagtgca actagaggta tggtacaaca gattccagga aaccagctct<br /> 9721 catcgccttc atctcatctt tcttcctttg catcaagctc agcgccattt gcatcctcct<br /> 9781 cttcggacac ctcaacaagt gagcatccag tgcctaatga agaggcggca ggtatggtac<br /> 9841 ttcaaataat tttctcccga tttaatcatc tcaagatgag gttcacttct ttatttgaat<br /> 9901 ataccattat aaggaaatag atcatgcgcc ctcttgttta aaggaatttg atgttttttt<br /> 9961 atattcgctc tctttgagat atacctgtca tccagcagtg gtaaaaagga tagtatatag<br /> 10021 ctgacaaata tgttcatata atttccttgt gggtaatttt gatattctct ttctatctta<br /> 10081 atacgtgtta tgagcaccgg ctaatgaccg aatggggtat gaatggaaca ccggaggtcg<br /> 10141 aggcttttgg cagcctcttc gacgtctggc ccatgatcgg cgacgaaagc aaaagcaggg<br /> 10201 gatgagagag tagagaattg gagacgagac tatagattga atcttgcttg gttcattcat<br /> 10261 gatttcgtgg cccttaatta ggcttacgat tgaactgaat tagctaataa aaaaggataa<br /> 10321 caaagtcttc tctaaatgta agcaatcgga tcactatcgc tcgtccggca tgccgcgtca<br /> 10381 gttttcagct cgcgtgatct ccttcgacgc cgcttggacc ggttgctgca tccgcgtagt<br /> 10441 tgcgtttctt catgtagacg attccgaccc tttacattac aactacttgg aattctttga<br /> 10501 gtgttctaaa ataatttgga tagtggtaca tatgtggtta cattttctta atggtgacat<br /> 10561 atagccaagt tggactaaga ttaattttaa tttgtgtgga atgagaaaga gtcaagacag<br /> 10621 tttgatacgt agaagtttgc atattcagaa ccttatactg gtttgtcttg gtttattttc<br /> 10681 ttgacaatgc agctttgaca gttcctcggt ccaaagagaa atgcgacaac actcccatgc<br /> 10741 cggtaaaaga tggcctgata tctgaagatg atgcaccggt acatctgcat cagaagcccc<br /> 10801 tgcaggcgac agccatggca gccatatgac tgacctgtaa tcctacaaga aaccaactga<br /> 10861 agattcatat gtggactgag tgaaattatg aaagttattg gaataaattg ttgctctgta<br /> 10921 tgtgagagca agcttcagtc cgttagcctg gttcctttta gtagtgttct actattggga<br /> 10981 gatcttcatc aacattttac atgaaacgtg atgtaatgaa cctctgttaa ttgttaattg<br /> 11041 tcagagctct agttttttgt ggtataaatt ttgttgcaag ggggcaatgc actcaacttt<br /> 11101 tgaatcattt gattggtatg gaaatcattg catttgtgca gagttcaggc agagttcagt<br /> 11161 tcttgcattt gcacatcgta ttactattac ctggccttgt ttggcacatc tccagttcca<br /> 11221 gctccacctc tcctagagct ggagctcagc caaacagttt cagctccacc gaaaatggga<br /> 11281 gcggagctgg gtggagcact ctaacaaaat gaactagaga ggtggagcta ggttaagctg<br /> 11341 ttccacaact ccacttcaga tctaactcct aaagttaaat ttaaaagttg aagctctacc<br /> 11401 aaacgagaaa acggacgata tgccactgta taggttgggt tcgaatgaaa tgccactcaa<br /> 11461 tacaatgtat cggataaaat gccactcaat acgttgccta ttggatgaaa tgccac<br /> &lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001059220.1 RefSeq:Os04g0401000]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 4]]<br /> [[Category:Chromosome 4]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=173080 Os09g0327600 2014-05-27T01:27:16Z <p>Victor: </p> <hr /> <div> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]<br /> ==References==<br /> &lt;references&gt;<br /> &lt;ref name=&quot;ref1&quot;&gt;<br /> S.Fukuoka;K.Okuno QTL analysis and mapping of pi21, a recessive gene for field resistance to rice blast in Japanese upland rice Theoretical and Applied Genetics, 2001, 103(2-3): 185-190<br /> &lt;/ref&gt;<br /> &lt;ref name=&quot;ref2&quot;&gt;<br /> Shuichi Fukuoka;Norikuni Saka;Hironori Koga;Kazuko Ono;Takehiko Shimizu;Kaworu Ebana;Nagao Hayashi;Akira Takahashi;Hirohiko Hirochika;Kazutoshi Okuno;Masahiro Yano Loss of Function of a Proline-Containing Protein Confers Durable Disease Resistance in Rice Science, 2009, 325(5943): 998-1001<br /> &lt;/ref&gt;<br /> &lt;/references&gt;<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os04g0401000|<br /> Description = Heavy metal transport/detoxification protein domain containing protein|<br /> Version = NM_001059220.1 GI:115458169 GeneID:4335725|<br /> Length = 1679 bp|<br /> Definition = Oryza sativa Japonica Group Os04g0401000, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|<br /> AP = Chromosome 4:19856181..19857859|<br /> CDS = 19856474..19856539,19856708..19857192,19857329..19857410|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008397:19856181..19857859<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008397:19856181..19857859<br /> source=RiceChromosome04<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgggtatattggtcatcttggtggacctgcaatgctgccgctgcgatgccaagatcaggaaggtcctgggctgccttgaagaggagtactgcatcgagaaggtggagtacgacgtgaagaacaacagggtgatcgtgcgcgggaagttcgacccggagaagctgtgcaagaagatctggtgcaaggccggcaagatcatcaaggagatcctcatcgtcgacgtctggccgccgccgctgccgcagccgccgccgccgtgcaagccgccgccgtgcgagaagcctccggaggactgcaagcccaagccctgccattgctgcagctgcgagaagcccaagcccaagcccaagccctgccactgcgagaagcccaagccctgtcactgcgagaagcccaagccatgcgagaagccgccgccgtgcaagccggaggagccgccgaagccgccgccggagaagccgccgccgaagccggagtgcaagctggtgccgtacccttacccggtgccgtacccgtacgccgggcagtggtgctgcccaaagcctgagccgccgaagccgccgccgcccgtctgcccgccgccgccgtggtgctacaccgaggacaacgccaacgcctgctccatcatgtga&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MGILVILVDLQCCRCDAKIRKVLGCLEEEYCIEKVEYDVKNNRV IVRGKFDPEKLCKKIWCKAGKIIKEILIVDVWPPPLPQPPPPCKPPPCEKPPEDCKPK PCHCCSCEKPKPKPKPCHCEKPKPCHCEKPKPCEKPPPCKPEEPPKPPPEKPPPKPEC KLVPYPYPVPYPYAGQWCCPKPEPPKPPPPVCPPPPWCYTEDNANACSIM&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;1321..1386#668..1152#450..531#atgcatcgcctccattattcatgcttcgattctccatgctttcctctactgctcattggtaacattcggcaaatttgacaggtgagctcagtattttaaatcttaatgtagtactttggtgtgctaatctttgctctgttcaaaagagaattctggtttctttgctattttgaaaagagaattttcgttacaggacttcaacttccataatacttttttttataattaaggcatgatatatatcttctttctgaattccacgggaattgcactttttcctgagaaatttgtaaagagcatgcctgttaattgcaaggggcccctaactctgttatgagaaaagagaactatataagatgctcaataagcacctctttttttttttctgttaactgaccaaagcctgtctatctgcatttttttttgttttttgtttttcttgtgtgcagatgggtatattggtcatcttggtggacctgcaatgctgccgctgcgatgccaagatcaggaaggtcctgggctgccttgaaggtataataaattctgcccgaatcgtccatgtttgattgaattttcaaggctaatcagcagtgttcctgctcaattgggagcaaaacctctgttaaaaagggtgtgtttgaatgaatataattgaatatgaacgcagaggagtactgcatcgagaaggtggagtacgacgtgaagaacaacagggtgatcgtgcgcgggaagttcgacccggagaagctgtgcaagaagatctggtgcaaggccggcaagatcatcaaggagatcctcatcgtcgacgtctggccgccgccgctgccgcagccgccgccgccgtgcaagccgccgccgtgcgagaagcctccggaggactgcaagcccaagccctgccattgctgcagctgcgagaagcccaagcccaagcccaagccctgccactgcgagaagcccaagccctgtcactgcgagaagcccaagccatgcgagaagccgccgccgtgcaagccggaggagccgccgaagccgccgccggagaagccgccgccgaagccggagtgcaagctggtgccgtacccttacccggtgccgtacccgtacgccgggcagtggtgctgcccaaagcctgagccgccgaagccgccgccggagccaccgaaggagccggagccgccgaagccgtgcgggtgctcgcacgccttcgtgtgcgtctgcaagccggcgccgccgccgccgccgccgtgcgggtgctcggggggccacgggaactgcggctgcggcatcaggccgtggccgccgcaggtgtggccgccgccgcccgtctgcccgccgccgccgtggtgctacaccgaggacaacgccaacgcctgctccatcatgtgatggccggccggcggtcggcgtcgatcacgatcatctctgctgcttaatttccttgcttgctactacctctgctcctttccttgcctcggaaatcggaataaattaaacacgaggctgatcgatgtgtttgtaattaatccatggtgtttgtgttgtgtgctgtgtgggctgtataataattaattacagtatgttcatgtaaatttgtttgtttgtttgtttatgttgttcgatatgtataattatgtacaataattaatcgtggagaggctcactaaactcataaactgtag&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001059220.1 RefSeq:Os04g0401000]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 4]]<br /> [[Category:Chromosome 4]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=172163 Os09g0327600 2014-05-25T06:21:56Z <p>Victor: </p> <hr /> <div> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:1.jpg]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:1.jpg&diff=172161 File:1.jpg 2014-05-25T06:20:23Z <p>Victor: uploaded a new version of &amp;quot;File:1.jpg&amp;quot;</p> <hr /> <div>Gross morphology of GID1-overexpressor and control plants. Scale<br /> bar, 50 cm.</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=172131 Os09g0327600 2014-05-25T05:44:13Z <p>Victor: </p> <hr /> <div> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.<br /> [[File:C:\Users\Administrator\Desktop\GEN18141627f3.jpg]]</div> Victor https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0327600&diff=172119 Os09g0327600 2014-05-25T05:26:25Z <p>Victor: Created page with &quot; Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice. To understand the molecular basis of Pi5-mediated resistance to ''...&quot;</p> <hr /> <div> Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.</div> Victor