BGIOSGA033504

2014-06-02T11:04:02Z

Wangll:

csld4:biosynthesis of cell walls and plant growth

==Annotated Information==
[[File:Figure 1.jpg|200px|thumb|right|Fig.1.Vegetative phenotypes of wild-type and mutation]]
[[File:Figure 2.png|200px|thumb|right|Fig.2. Development of the third-leaf blade in wild-type and mutation.]]
[[File:Figure 3.jpg|200px|thumb|right|Fig.3. Reproductive phenotypes of wild-type and mutation .]]
[[File:Figure 4.png|200px|thumb|right|Fig.4.In situhybridization of OsCSLD4in longitudinal sections of 10-d-old shoot apex]]
[[File:Figure 5.jpg|200px|thumb|right|Fig.5.Double-target in situhybridization of OsCSLD4and CDKB2;1in the shoot apex of wild-type plants. ]]

===Function===
Cellulose synthase-like (CSL) genes are predicted to catalyse the biosynthesis of non-cellulosic polysaccharides such as the β-d-glycan backbone of hemicelluloses and are classified into nine subfamilies (CSLA–CSLH and CSLJ). The CSLD subfamily is conserved in all land plants, and among the nine CSL subfamilies, it shows the highest sequence similarity to the cellulose synthase genes, suggesting that it plays fundamental roles in plant development. The mutants of csld4 showed rolled and narrow leaf blades and a reduction in plant height（figure1）. The narrow leaf blade of sle1 was caused by reduced cell proliferation beginning at the P3 primordial stage （figure 2）. In addition to the size reduction of organs, sle1 mutants exhibited serious developmental defects in pollen formation , anther dehiscence（figure 3）, stomata formation, and cell arrangement in various tissues.

===Expression===
In situ hybridization experiments showed that OsCSLD4 was expressed in a patchy pattern in developing organs（figure 4）. Double-target in situ hybridization and quantitative RT-PCR analyses revealed that SLE1 was expressed specifically during the M-phase of the cell cycle（figure 5）, and suggested that the cell-cycle regulation was altered in sle1 mutants. These results suggest that the OsCSLD4 protein plays a pivotal role in the M phase to regulate cell proliferation.

==Labs working on this gene==
1 Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113–8657, Japan
2 National Institute of Genetics, 1111 Yata, Mishima, Shizuoka, 411–8540, Japan

==References==
1. Takanori Yoshikawa;Mitsugu Eiguchi;Ken-Ichiro Hibara;Jun-Ichi Ito;Yasuo Nagato, Rice SLENDER LEAF 1 gene encodes cellulose synthase-like D4 and is specifically expressed in M-phase cells to regulate cell proliferation. Journal of Experimental Botany, 2013, 64(7): 2049-2061
2. Jiang Hu;Li Zhu;Dali Zeng;Zhenyu Gao;Longbiao Guo;Yunxia Fang;Guangheng Zhang;Guojun Dong;Meixian Yan;Jian Liu;Qian Qian.. Identification and characterization of NARROW AND ROLLED LEAF 1, a novel gene regulating leaf morphology and plant architecture in rice. Plant Molecular Biology, 2010, 73(3): 283-292
3. Chao Wu;Yaping Fu;Guocheng Hu;Huamin Si;Shihua Cheng;Wenzhen Liu. Isolation and characterization of a rice mutant with narrow and rolled leaves. Planta, 2010, 232(2): 313-324

==Structured Information==
{{IndicaGene|
GeneName = BGIOSGA033504|
Description = Csld4-cellulose synthase-like family d|
Definition = Oryza sativa Indica Group BGIOSGA033504, complete gene.|
tag = OSJNBA0056G17.16|
tid = BGIOSGA033504-TA|
pid = BGIOSGA033504-PA|
Length = 3474|
Source = Oryza sativa Indica Group

ORGANISM Oryza sativa Indica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Indica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:22128267..22131740|
CDS = 22128267..22130942,22131033..22131740|
GCID = <gbrowseImage1>
name=IndicaChromosome10:22128267..22131740
source=RiceIndica10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=IndicaChromosome10:22128267..22131740
source=RiceIndica10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>ATGGCGTCGAAGGGCATCCTCAAGAACGGCGGTAAGCCGCCGACGGCGCCATCGTCGGCGGCGCCCACGGTGGTGTTCGGTCGGCGGACGGACTCGGGGCGATTCATCAGCTACTCCCGCGACGACCTTGACTCGGAGATCAGCAGCGTGGACTTCCAGGACTACCACGTCCACATCCCCATGACGCCGGACAACCAGCCCATGGACCCTGCCGCCGGCGACGAGCAGCAGTACGTGTCCAGCTCGCTCTTCACCGGCGGGTTCAACAGCGTGACACGCGCGCACGTGATGGAGAAGCAGGCGTCGTCGGCGAGGGCGACGGTGTCGGCGTGCATGGTGCAGGGGTGCGGGTCCAAGATCATGCGCAACGGGCGCGGCGCCGACATCCTCCCCTGCGAGTGCGACTTCAAGATCTGCGTGGACTGCTTCACCGACGCCGTCAAGGGCGGCGGCGGCGTGTGCCCGGGGTGCAAGGAGCCGTACAAGCACGCGGAGTGGGAGGAGGTGGTGTCGGCGTCCAACCACGACGCCATCAACAGGGCGCTGTCGCTGCCGCACGGGCACGGGCATGGCCCCAAGATGGAGAGGCGGCTGTCGCTGGTGAAGCAGAACGGGGGGGCGCCCGGCGAGTTCGACCACAACCGCTGGCTCTTCGAGACCAAGGGCACCTACGGCTACGGCAACGCAATCTGGCCCGAGGACGACGGCGTGGCGGGGCACCCCAAGGAGCTGATGAGCAAGCCATGGCGGCCGCTGACCCGCAAGCTCCGGATCCAGGCGGCGGTGATCAGCCCGTACAGGCTGCTGGTCCTGATCCGGCTGGTGGCGCTGGGGTTGTTCCTGATGTGGCGCATCAAGCACCAGAACGAGGACGCCATCTGGCTGTGGGGGATGTCCATCGTGTGCGAGCTGTGGTTCGCCTTGTCGTGGGTGCTGGACCAGCTGCCCAAGCTGTGCCCCATCAACCGCGCCACCGACCTGAGCGTGCTCAAGGACAAGTTCGAGACGCCCACGCCGAGCAACCCCACCGGCAAGTCCGACCTCCCCGGGATCGACATCTTCGTCTCCACCGCCGACCCGGAGAAGGAGCCCGTGCTGGTGACGGCCAACACCATCCTCTCCATCCTGGCCGCCGACTACCCCGTCGACAAGCTGGCGTGCTACGTGTCCGACGACGGCGGGGCGCTGCTGACGTTCGAGGCCATGGCGGAGGCGGCGAGCTTCGCCAACCTGTGGGTGCCATTCTGCCGGAAGCACGAGATCGAGCCGAGGAACCCCGACAGCTACTTCAACCTCAAGAGGGACCCGTTCAAGAACAAGGTGAAGGGCGACTTCGTCAAGGACAGGCGGCGGGTGAAGCGGGAGTACGACGAGTTCAAGGTCCGCGTCAATGGCCTCCCCGACGCCATCCGCCGCCGCTCCGACGCGTACCACGCCCGCGAGGAGATCCAGGCCATGAACCTGCAGCGGGAGAAGATGAAGGCCGGCGGCGACGAGCAGCAGCTGGAGCCGATAAAGATCCCCAAGGCGACGTGGATGGCGGACGGCACGCACTGGCCGGGGACGTGGCTGCAGGCGTCGCCGGAGCACGCGAGGGGCGACCACGCGGGGATCATACAGGTGATGCTAAAGCCTCCCAGTCCCAGTCCCAGCAGCAGCGGCGGCGACATGGAAAAGAGGGTGGATTTGTCGGGCGTGGACACCCGGCTGCCGATGCTGGTGTACGTGTCGCGGGAGAAGCGGCCGGGGTACGACCACAACAAGAAGGCGGGCGCCATGAACGCGCTGGTGCGGGCGTCGGCGATCATGTCCAACGGGCCGTTCATCCTCAACCTGGACTGCGACCACTACGTGTACAACTCCAAGGCGTTCCGGGAGGGGATGTGCTTCATGATGGACCGCGGCGGCGACCGCCTCTGCTACGTCCAGTTCCCGCAGCGCTTCGAGGGCATCGACCCCTCCGACCGCTACGCCAACCACAACACCGTCTTCTTCGACGTCAACATGCGCGCCCTCGACGGCCTCCAGGGCCCCGTCTATGTCGGAACTGGCTGCCTCTTCCGCCGCATCGCGCTCTACGGCTTCGACCCGCCCAGGTCCAAGGACCACACCACCCCGTGGTCCTGCTGCCTGCCACGTCGCCGCCGAACAAGATCCCAGCCCCAGCCCCAGGAGGAGGAGGAGGAGACGATGGCCCTCCGCATGGACATGGACGGCGCGATGAACATGGCGAGCTTCCCCAAGAAGTTCGGCAACTCGAGCTTCCTGATCGACTCCATCCCGGTGGCGGAGTTCCAGGGACGGCCGCTGGCGGACCACCCGTCGGTGAAGAACGGGCGGCCGCCGGGGGCGCTGACGATCCCGCGGGAGACGCTGGACGCGTCGATCGTGGCGGAGGCGATCAGCGTGGTGTCGTGCTGGTACGAGGAGAAGACGGAGTGGGGGACGAGGGTGGGTTGGATATACGGGTCGGTGACGGAGGACGTGGTCACCGGCTACCGCATGCACAACCGGGGGTGGAAGTCCGTCTACTGCGTCACCCACCGCGACGCCTTCCGCGGCACCGCCCCCATCAACCTCACCGACCGCCTCCACCAGGTCCTCCGATGGGCCACTGGCTCCGTCGAGATCTTCTTCTCACGCAACAACGCACTCTTCGCTTCCTCAAAGATGAAGGTTCTCCAGCGCATCGCTTACCTCAACGTTGGCATCTACCCCTTCACCTCCGTCTTCCTCATCGTCTACTGCTTCCTCCCGGCGCTCTCCCTCTTCTCGGGGCAGTTCATCGTGCAGACGCTCAACGTCACCTTCCTCACCTACCTGCTCATCATCACCATCACGCTCTGCCTCCTGGCCATGCTGGAGATCAAGTGGTCCGGCATCGCGCTGGAGGAGTGGTGGCGGAACGAGCAGTTCTGGCTCATCGGCGGAACCAGCGCCCACCTCGCCGCCGTCCTGCAGGGCCTTCTCAAGGTCATCGCCGGCATCGAGATCTCCTTCACCCTCACCTCCAAGCAGCTCGGCGACGACGTCGACGACGAGTTCGCCGAGCTCTACGCGGTCAAGTGGACGTCCCTCATGATACCGCCGCTCACCATCATAATGATCAACCTCGTCGCCATCGCCGTCGGCTTCAGCCGCACCATCTACAGCACCATCCCGCAGTGGAGCAAGCTTCTCGGTGGAGTCTTCTTCAGCTTCTGGGTCCTCGCGCATCTCTACCCCTTCGCCAAGGGCCTCATGGGCAGGAGGGGAAGGACACCCACCATCGTCTACGTCTGGTCTGGCCTCGTCGCCATCACCATCTCCTTGCTCTGGATTGCAATCAAGCCTCCTTCTGCACAAGCCAACTCACAGCTAGGAGGATCCTTCTCTTTCCCCTGA</cdnaseq>|
AA = <aaseq>MASKGILKNGGKPPTAPSSAAPTVVFGRRTDSGRFISYSRDDLDSEISSVDFQDYHVHIPMTPDNQPMDPAAGDEQQYVSSSLFTGGFNSVTRAHVMEKQASSARATVSACMVQGCGSKIMRNGRGADILPCECDFKICVDCFTDAVKGGGGVCPGCKEPYKHAEWEEVVSASNHDAINRALSLPHGHGHGPKMERRLSLVKQNGGAPGEFDHNRWLFETKGTYGYGNAIWPEDDGVAGHPKELMSKPWRPLTRKLRIQAAVISPYRLLVLIRLVALGLFLMWRIKHQNEDAIWLWGMSIVCELWFALSWVLDQLPKLCPINRATDLSVLKDKFETPTPSNPTGKSDLPGIDIFVSTADPEKEPVLVTANTILSILAADYPVDKLACYVSDDGGALLTFEAMAEAASFANLWVPFCRKHEIEPRNPDSYFNLKRDPFKNKVKGDFVKDRRRVKREYDEFKVRVNGLPDAIRRRSDAYHAREEIQAMNLQREKMKAGGDEQQLEPIKIPKATWMADGTHWPGTWLQASPEHARGDHAGIIQVMLKPPSPSPSSSGGDMEKRVDLSGVDTRLPMLVYVSREKRPGYDHNKKAGAMNALVRASAIMSNGPFILNLDCDHYVYNSKAFREGMCFMMDRGGDRLCYVQFPQRFEGIDPSDRYANHNTVFFDVNMRALDGLQGPVYVGTGCLFRRIALYGFDPPRSKDHTTPWSCCLPRRRRTRSQPQPQEEEEETMALRMDMDGAMNMASFPKKFGNSSFLIDSIPVAEFQGRPLADHPSVKNGRPPGALTIPRETLDASIVAEAISVVSCWYEEKTEWGTRVGWIYGSVTEDVVTGYRMHNRGWKSVYCVTHRDAFRGTAPINLTDRLHQVLRWATGSVEIFFSRNNALFASSKMKVLQRIAYLNVGIYPFTSVFLIVYCFLPALSLFSGQFIVQTLNVTFLTYLLIITITLCLLAMLEIKWSGIALEEWWRNEQFWLIGGTSAHLAAVLQGLLKVIAGIEISFTLTSKQLGDDVDDEFAELYAVKWTSLMIPPLTIIMINLVAIAVGFSRTIYSTIPQWSKLLGGVFFSFWVLAHLYPFAKGLMGRRGRTPTIVYVWSGLVAITISLLWIAIKPPSAQANSQLGGSFSFP</aaseq>|
DNA = <dnaseqindica>1..2676#2767..3474#ATGGCGTCGAAGGGCATCCTCAAGAACGGCGGTAAGCCGCCGACGGCGCCATCGTCGGCGGCGCCCACGGTGGTGTTCGGTCGGCGGACGGACTCGGGGCGATTCATCAGCTACTCCCGCGACGACCTTGACTCGGAGATCAGCAGCGTGGACTTCCAGGACTACCACGTCCACATCCCCATGACGCCGGACAACCAGCCCATGGACCCTGCCGCCGGCGACGAGCAGCAGTACGTGTCCAGCTCGCTCTTCACCGGCGGGTTCAACAGCGTGACACGCGCGCACGTGATGGAGAAGCAGGCGTCGTCGGCGAGGGCGACGGTGTCGGCGTGCATGGTGCAGGGGTGCGGGTCCAAGATCATGCGCAACGGGCGCGGCGCCGACATCCTCCCCTGCGAGTGCGACTTCAAGATCTGCGTGGACTGCTTCACCGACGCCGTCAAGGGCGGCGGCGGCGTGTGCCCGGGGTGCAAGGAGCCGTACAAGCACGCGGAGTGGGAGGAGGTGGTGTCGGCGTCCAACCACGACGCCATCAACAGGGCGCTGTCGCTGCCGCACGGGCACGGGCATGGCCCCAAGATGGAGAGGCGGCTGTCGCTGGTGAAGCAGAACGGGGGGGCGCCCGGCGAGTTCGACCACAACCGCTGGCTCTTCGAGACCAAGGGCACCTACGGCTACGGCAACGCAATCTGGCCCGAGGACGACGGCGTGGCGGGGCACCCCAAGGAGCTGATGAGCAAGCCATGGCGGCCGCTGACCCGCAAGCTCCGGATCCAGGCGGCGGTGATCAGCCCGTACAGGCTGCTGGTCCTGATCCGGCTGGTGGCGCTGGGGTTGTTCCTGATGTGGCGCATCAAGCACCAGAACGAGGACGCCATCTGGCTGTGGGGGATGTCCATCGTGTGCGAGCTGTGGTTCGCCTTGTCGTGGGTGCTGGACCAGCTGCCCAAGCTGTGCCCCATCAACCGCGCCACCGACCTGAGCGTGCTCAAGGACAAGTTCGAGACGCCCACGCCGAGCAACCCCACCGGCAAGTCCGACCTCCCCGGGATCGACATCTTCGTCTCCACCGCCGACCCGGAGAAGGAGCCCGTGCTGGTGACGGCCAACACCATCCTCTCCATCCTGGCCGCCGACTACCCCGTCGACAAGCTGGCGTGCTACGTGTCCGACGACGGCGGGGCGCTGCTGACGTTCGAGGCCATGGCGGAGGCGGCGAGCTTCGCCAACCTGTGGGTGCCATTCTGCCGGAAGCACGAGATCGAGCCGAGGAACCCCGACAGCTACTTCAACCTCAAGAGGGACCCGTTCAAGAACAAGGTGAAGGGCGACTTCGTCAAGGACAGGCGGCGGGTGAAGCGGGAGTACGACGAGTTCAAGGTCCGCGTCAATGGCCTCCCCGACGCCATCCGCCGCCGCTCCGACGCGTACCACGCCCGCGAGGAGATCCAGGCCATGAACCTGCAGCGGGAGAAGATGAAGGCCGGCGGCGACGAGCAGCAGCTGGAGCCGATAAAGATCCCCAAGGCGACGTGGATGGCGGACGGCACGCACTGGCCGGGGACGTGGCTGCAGGCGTCGCCGGAGCACGCGAGGGGCGACCACGCGGGGATCATACAGGTGATGCTAAAGCCTCCCAGTCCCAGTCCCAGCAGCAGCGGCGGCGACATGGAAAAGAGGGTGGATTTGTCGGGCGTGGACACCCGGCTGCCGATGCTGGTGTACGTGTCGCGGGAGAAGCGGCCGGGGTACGACCACAACAAGAAGGCGGGCGCCATGAACGCGCTGGTGCGGGCGTCGGCGATCATGTCCAACGGGCCGTTCATCCTCAACCTGGACTGCGACCACTACGTGTACAACTCCAAGGCGTTCCGGGAGGGGATGTGCTTCATGATGGACCGCGGCGGCGACCGCCTCTGCTACGTCCAGTTCCCGCAGCGCTTCGAGGGCATCGACCCCTCCGACCGCTACGCCAACCACAACACCGTCTTCTTCGACGTCAACATGCGCGCCCTCGACGGCCTCCAGGGCCCCGTCTATGTCGGAACTGGCTGCCTCTTCCGCCGCATCGCGCTCTACGGCTTCGACCCGCCCAGGTCCAAGGACCACACCACCCCGTGGTCCTGCTGCCTGCCACGTCGCCGCCGAACAAGATCCCAGCCCCAGCCCCAGGAGGAGGAGGAGGAGACGATGGCCCTCCGCATGGACATGGACGGCGCGATGAACATGGCGAGCTTCCCCAAGAAGTTCGGCAACTCGAGCTTCCTGATCGACTCCATCCCGGTGGCGGAGTTCCAGGGACGGCCGCTGGCGGACCACCCGTCGGTGAAGAACGGGCGGCCGCCGGGGGCGCTGACGATCCCGCGGGAGACGCTGGACGCGTCGATCGTGGCGGAGGCGATCAGCGTGGTGTCGTGCTGGTACGAGGAGAAGACGGAGTGGGGGACGAGGGTGGGTTGGATATACGGGTCGGTGACGGAGGACGTGGTCACCGGCTACCGCATGCACAACCGGGGGTGGAAGTCCGTCTACTGCGTCACCCACCGCGACGCCTTCCGCGGCACCGCCCCCATCAACCTCACCGACCGCCTCCACCAGGTCCTCCGATGGGCCACTGGCTCCGTCGAGATCTTCTTCTCACGCAACAACGCACTCTTCGCTTCCTCAAAGATGAAGGTGCCTAGCTTGCTATTCGATCGATTATTTTTCTTCTTCTTCTTCTTCTTCCGCTGCCGCCAAACAAACAATTATCCATTGCATTTGCAGGTTCTCCAGCGCATCGCTTACCTCAACGTTGGCATCTACCCCTTCACCTCCGTCTTCCTCATCGTCTACTGCTTCCTCCCGGCGCTCTCCCTCTTCTCGGGGCAGTTCATCGTGCAGACGCTCAACGTCACCTTCCTCACCTACCTGCTCATCATCACCATCACGCTCTGCCTCCTGGCCATGCTGGAGATCAAGTGGTCCGGCATCGCGCTGGAGGAGTGGTGGCGGAACGAGCAGTTCTGGCTCATCGGCGGAACCAGCGCCCACCTCGCCGCCGTCCTGCAGGGCCTTCTCAAGGTCATCGCCGGCATCGAGATCTCCTTCACCCTCACCTCCAAGCAGCTCGGCGACGACGTCGACGACGAGTTCGCCGAGCTCTACGCGGTCAAGTGGACGTCCCTCATGATACCGCCGCTCACCATCATAATGATCAACCTCGTCGCCATCGCCGTCGGCTTCAGCCGCACCATCTACAGCACCATCCCGCAGTGGAGCAAGCTTCTCGGTGGAGTCTTCTTCAGCTTCTGGGTCCTCGCGCATCTCTACCCCTTCGCCAAGGGCCTCATGGGCAGGAGGGGAAGGACACCCACCATCGTCTACGTCTGGTCTGGCCTCGTCGCCATCACCATCTCCTTGCTCTGGATTGCAATCAAGCCTCCTTCTGCACAAGCCAACTCACAGCTAGGAGGATCCTTCTCTTTCCCCTGA</dnaseqindica>|
Link = [http://plants.ensembl.org/Oryza_indica/Gene/Summary?g=BGIOSGA033504 EnsemblPlants:BGIOSGA033504]|
}}
[[Category:Genes]]
[[Category:Indica mRNA]]
[[Category:Oryza Sativa Indica Group]]
[[Category:Indica Genes]]
[[Category:Indica Chromosome 10]]
[[Category:Chromosome 10]]

File:Figure 5.jpg

2014-06-02T10:49:42Z

Wangll: uploaded a new version of "File:Figure 5.jpg"

Expression pattern ofGS3.(A–H) Expression ofGS3in various tissues
revealed by in situ hybridization: (A) panicle at stage of pollen mother cell
formation, (B) panicle at pollen mother cell meiosis, (C) panicle at pollen grain
filling, (D) root at trefoil stage, (E) stem at heading stage, (F) leaf at tillering
stage, (G) endosperm and embryo 14 d afterflowering, and (H) longitudinal
section of 7-d-old seedling. (I) Negative control by hybridizing the young
Figure 5:panicle with the sense probe. All tissues were sampled from Zhenshan 97
(medium grain). (Scale bars, 200 μm.) (J) Comparative expression pattern of
GS3in Minghui 63 and NIL(c7) in various organs using real-time RT-PCR
analysis: S, seedling at trefoil stage; R, root at trefoil stage; L, leaf at tillering
stage; St, stem at heading stage; P1, young panicle<1 cm in length; P2, young
panicle<5 cm in length; P3, young panicle 5–10 cm in length; P4, panicle
before heading>10 cm in length; P5, panicle 5 d after heading; Pl, plumule

File:Figure 4.png

2014-06-02T10:49:29Z

Wangll: uploaded a new version of "File:Figure 4.png"

Phylogenetic Tree of An-1 in Rice

File:Figure 3.jpg

2014-06-02T10:49:18Z

Wangll: uploaded a new version of "File:Figure 3.jpg"

Figure 3: GUS staining of pistils transformed with the GS3promoter::GUSfusion construct and development of the stigma. (A) Pistils
from panicles approximately 7 cm, 10 cm, 13 cm and 15 cm long and
after heading. Scale bar, 500µm. (B) Relationship between panicle
length and SNBP (stigma non-brush-shaped part) length. Error bars
indicate SE (n=4).

File:Figure 2.png

2014-06-02T10:49:08Z

Wangll: uploaded a new version of "File:Figure 2.png"

Grain Length among between different plants

File:Figure 1.jpg

2014-06-02T10:48:46Z

Wangll: uploaded a new version of "File:Figure 1.jpg"