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Os06g0493100

2014-05-28T01:22:40Z

WilliamE: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==
===Function===
The ''Brown planthopper induced008a'' (''Bphi008a''; AY256682) gene of rice (''Oryza sativa'') enhances the plant’s resistance to the brown planthopper (BPH; ''Nilaparvata lugens''). BPH feeding rapidly initiated the ethylene (ET) signaling pathway and up-regulated ''Bphi008a'' transcript levels after 6 to 96 h of feeding. In contrast, blocking ethylene transduction (using 1-methylcyclopropene) reduced ''Bphi008a'' transcript levels in wild-type plants fed upon by BPH. Because ''Bphi008a'' is a downstream gene of the Et signaling pathway in rice. The overexpressed (OE) plants had greater resistance to the BPH than wildtype plants (Fig. 1wD). In contrast, the change in susceptibility of rice to the BPH caused by RNAi of ''Bphi008a'' was not obvious. The level of ''Bphi008a'' expression affects BPH feeding on the phloem of rice (Fig. 1wE). The ''Bphi008a'' expression enhances rice resistance to the BPH by impairing BPH feeding (Fig.1wF). The ''Bphi008a'' can be phosphorylated by rice Mitogen-activated Protein Kinase5 (OsMPK5). Yeast two-hybrid assays demonstrated that the carboxyl-terminal proline-rich region of ''Bphi008a'' interacts directly with this kinase, and this interaction occurs in the nucleus. Subsequently, we found that ''Bphi008a'' up-regulation and down-regulation were accompanied by different changes in transcription levels of OsMPK5, OsMPK12, OsMPK13, and OsMPK17 in transgenic plants (Fig. 2w). Immunoblot analysis also showed that the OsMPK5 protein level increased in overexpressing plants and decreased in RNA interference plants after BPH feeding(Fig. 3w, A and B). The ''Bphi008a'' Overexpression Enhances Levels of AP2/ERF Transcription Factors and Defense-Related Genes. Yeast two-hybrid screening results showed that a clear interaction between ''Bphi008a''and a b-ZIP transcription factor (OsbZIP60), but a weak interaction between ''Bphi008a'' and a RNA polymerase polypeptide (SDRP) (Fig. 4wB). Furthermore, analysis of OsbZIP60 expression levels in wild-type and transgenic plants after BPH feeding from 0 to 96 h (Fig. 4wD) indicated that ''Bphi008a'' (possibly phosphorylated) might form a transcriptional complex with OsbZIP60 and SDRP in vivo that activates the transcription of target genes.<ref name="ref1" />[[File:Fig.1w.png]][[File:Fig.2w.png]][[File:Fig.3w.png]][[File:Fig.4w.png]]

===Expression===
In a previous study, ''Bphi008a'' expression was found to be induced by both BPH feeding and spraying plants with ethephon, which slowly releases Et.<ref name="ref2" />.In rice, a large gene family controls Et biosynthesis, composed of six 1-aminocyclopropane-1-carboxylic acid (ACC) synthase genes (designated OsACS1–OsACS6) and
seven ACC oxidase genes (designated OsACO1–OsACO7)<ref name="ref3" /><ref name="ref4" />. Experimental results indicate that Bphi008a transcription is stimulated by active Et(Fig. 5w).Bphi008a expression levels were highest in the roots of seedlings, followed (in order) by leaf blades and leaf sheaths at the heading stage, then leaf sheaths, leaf blades, and stems of seedlings. The lowest detected level of Bphi008a expression was in the flowers (Fig. 6w).[[File:Fig.5w.png]][[File:Fig.6w.png]]

===Evolution===
Please input evolution information here.

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==Labs working on this gene==
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, China.

College of Life Sciences, Xinyang Normal University, China.

==References==
<references>
<ref name="ref1">Jing Hu, Jiangbo Zhou, Xinxin Peng, Henghao Xu, Caixiang Liu, Bo Du, Hongyu Yuan, Lili Zhu, and Guangcun He. The Bphi008a Gene Interacts with the Ethylene Pathway and Transcriptionally Regulates MAPK Genes in the Response of Rice to Brown Planthopper Feeding1[C][W][OA][J].Plant Physiology, 2011, 156: 856-872.
</ref>
<ref name="ref2">Yuan H, Chen X, Zhu L, He G. Isolation and characterization of a novel rice gene encoding a putative insect-inducible protein homologous to wheat Wir1[J].Plant Physiol, 2004, 161: 79-85.
</ref>
<ref name="ref3">Yang SF, Hoffman NE. Ethylene biosynthesis and its regulation in higher plants[J]. Annu Rev Plant Physiol, 1984, 35: 155-189.
</ref>
<ref name="ref4">Iwai T, Miyasaka A, Seo S, Ohashi Y. Contribution of ethylene biosynthesis for resistance to blast fungus infection in young rice plants[J]. Plant Physiol, 2006, 142: 1202-1215.
</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0493100|
Description = Conserved hypothetical protein|
Version = NM_001064216.1 GI:115468163 GeneID:4341087|
Length = 1685 bp|
Definition = Oryza sativa Japonica Group Os06g0493100, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:17943931..17945615|
CDS = 17944169..17944311,17945468..17945573|
GCID = <gbrowseImage1>
name=NC_008399:17943931..17945615
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:17943931..17945615
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcgtctcttagagccaccacactccttgtgttgatgcaaattattatggtgttttacaccgtcatcttaagctgctcgttttctgatgcccgaactttcccaggcggagaaggtgggttagacccaaatcatccagtatgcgttggcggcgcgtgtccaacaccaggtctaccatacactaatccacgtgatccttgcatataccgtaacaggtgcaatccaccaggacggatgggtgatccataa</cdnaseq>|
AA = <aaseq>MASLRATTLLVLMQIIMVFYTVILSCSFSDARTFPGGEGGLDPN HPVCVGGACPTPGLPYTNPRDPCIYRNRCNPPGRMGDP</aaseq>|
DNA = <dnaseqindica>1305..1447#43..148#atatatcaagcatacacaagaaggatttattgaaggccaaccatggcgtctcttagagccaccacactccttgtgttgatgcaaattattatggtgttttacaccgtcatcttaagctgctcgttttctgatgcccgaactttcccaggtgagtgtgtcaatttttagcctttttttccttgagcaatatatattagttccaccgttctatactcaccccaccacggcataaaacttacaaggttgggttaaaatagaagggcgtggaatttctacatttttaacaaaagaatatatatatatatatatatatatatatatatatatatatatatatatatatatatatgtaggttagatataattgtgagataaccatcaactgttatgggcatatatatatccataaattgttatgttgacatatttgcacttcagtagtaacacctcagctactgtgtttacgtacagtgaaattctagttcttgaactttctaatgcaaaataatttaactactttaaaataaagtaaaccttccgtaagtattagcaaggccagactatctctattggcaaggctaagggtgcctttttttttaattatgattttggttgtttagctatacgtagaacgtgaaacatgattattatatgattagttgagtattaattattaaaacttacaaaatatatttatttaatttttaaaacattttctatatatagagtttttcacacaacgagacaaggatttaactatgctgtccatagtgttgtggcaagccaccatcgtcaatgtcgtcgtgccatgagcagagtagtactgtatagtaacttgccacaccaccgaaatatttatatttaaaaatatgtttttatgtggtactccctctatttcaggttataagatatttaagttttttttaccaaatttatagaaaaaaatagtaatattttcgacccaagataaatttattataaaaataaatttaattactaatttaataaaactaatttagtaatgtaaatattacaatatttatctataaatttaattaaacttaaagcagtttaactttagctaaagtcaaaacatcttataatctgaaatggatggaatatattttaaaaactacacgaaaaaggttaagaaaaatttacatcaccttgggtccatatatagatatctcgcatgctaactgtttggaaaaaaaaaccctgaaatatatataggcctggtaaatataatggcaaataacgtgttgctagttcgtctaatataatggcaaataaccaatgtacaggcggagaaggtgggttagacccaaatcatccagtatgcgttggcggcgcgtgtccaacaccaggtctaccatacactaatccacgtgatccttgcatataccgtaacaggtgcaatccaccaggacggatgggtgatccataagtaattttcatcatcaatatttatcgttgtgatccagtatacgtccacatcgtccacatgacaatagtgtgtgtttgtgtatgttatactagtattcactagctgctcacactgcatgagagctagttaattaagagaatgtctatgtatgacaaataatgtactgtggtactatatatgtatccccctgtctcggcaatatatggattaataagacatgtgtactctggatctgatt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001064216.1 RefSeq:Os06g0493100]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

2014-05-26T08:04:43Z

WilliamE:

File:Fig.1w.png

2014-05-26T08:03:40Z

WilliamE: Figure 1. Northern- and Southern-blot analysis of Bphi008a in wild-type and transgenic plants, and evaluation of its contribution to resistance to the BPH. A, Bphi008a expression levels in normal wild-type (WT) and six T0 OE plants. OE17 was a three-copy