http://192.168.164.12:81/ricewiki/api.php?action=feedcontributions&feedformat=atom&user=Wuxt 2026-05-25T04:29:15Z User contributions MediaWiki 1.30.0 https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0898300&diff=176664 2014-06-03T10:45:38Z

<p>Wuxt: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> OsSPR1 was identified by map-based cloning. It encodes a novel mitochondrial protein with the Armadillo-like repeat domain. Osspr1 mutants exhibited decreased root cell elongation. The large reduction of Fe content observed in the leaves but not the roots of Osspr1 mutant plants under Fe(II) and Fe(III) nutrient supply, and the normal Fe deficiency response for both strategy I and II marker genes in the leaves but not the roots indicate a defect in Fe homeostasis. In addition, root development was also compromised in Osspr1 mutant plants. It is likely that the defective roots of Osspr1 plants will have a secondary effect on nutrient uptake. However, if reduced Fe uptake as a result of damaged roots was the primary reason for the reduced Fe content in the leaves, the Fe content and the Fe deficiency response would have been duced and activated, respectively, in the roots. However, the Fe content in roots of Osspr1 mutant plants was similar to that of wild-type plants (Fig. 6e). Also, no Fe deficiency response was observed in roots (Fig. 7). However, a lower Fe content was observed in the mutant leaves, which was inconsistent with the induction of Fe-responsive genes (Figs 6c, 7). These results indicate that OsSPR1 plays a role in maintaining Fe (and other metal) homeostasis between roots and leaves.<br /> <br /> <br /> [[File:Fig.7.jpg]]<br /> [[File:Fig.6c.jpg]]<br /> [[File:Fig.6e.jpg]]<br /> <br /> ===Expression===<br /> <br /> The expression pattern of OsSPR1 was determined usingt he GUS gene driven by the OsSPR1 promoter transferred into wild-type plants. GUS staining in transgenic plants indicated that OsSPR1 was ubiquitously expressed in all plant organs, including the root, leaf, stem and spikelet (Fig. 3a–r). OsSPR1 was expressed in primary and lateral roots, both in mature regions and in root tips. Strong expression was observed in the root tip (in the meristem and lateral root initiation regions), where dividing cells are present. The expression of OsSPR1 in the root is mainly associated with vascular tissues (Fig. 3b,d,h). Semiquantitative RT-PCR analysis using SPR1-specific primers showed that SPR1 was expressed at relatively similar levelsin all tissues, including the root, stem base, stem, leaf and panicles (Fig. 3s), consistent with the GUS staining patterns observed.<br /> <br /> [[File:Fig.3.jpg]]<br /> <br /> ===Evolution===<br /> Root elongation is determined by two successive processes: cell elongation and cell proliferation (Beemster et al., 2003). In rice, several genes controlling division and differentiation in root growth and development have been identified through genetic screens (Rebouillat et al., 2009). Here, we cloned OsSPR1, a novel mitochondrial gene involved in root cell elongation. Loss of function of OsSPR1 resulted in defective root elongation, including primary root, adventitious root and lateral root elongation; notably, the defects were more severe in lateral roots and adventitious roots. Analysis of the different root zones revealed similar arrangements of cells in those zones compared with the wild type, but reduced cell length was observed in the spr1 roots,<br /> suggesting that reduced root length in spr1 resulted from reduced cell elongation.<br /> ==Labs working on this gene==<br /> *Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Ghent University, Belgium.<br /> *State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China <br /> *ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, 6009 WA, Australia<br /> *Joint Laboratory in Genomics and Nutriomics, College of Life Science, Zhejiang University, Hangzhou 310058, China<br /> *Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China<br /> <br /> ==References==<br /> 1.Beemster GTS, Fiorani F, Inze´ D. 2003. Cell cycle: the key to plant growth control? Trends in Plant Science 8: 154–158.<br /> <br /> 2.Rebouillat J, Dievart A, Verdeil J, Escoute J, Giese G, Breitler J, Gantet P, Espeout S, Guiderdoni E, Pe´rin C. 2009. Molecular genetics of rice root development. Rice 2: 15–34.<br /> <br /> 3.Liqiang Jia;Zhongchang Wu;Xi Hao;Chris Carrie;Libin Zheng;James Whelan;Yunrong Wu;Shoufeng Wang;Ping Wu;Chuanzao Mao. Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa.New Phytologist, 2011, 189(3): 843-855.<br /> <br /> 4.Jia LiQiang . Is Reactive Oxygen Species (ROS) the underlying factor for inhibited root growth in Osspr1? Plant Signaling &amp; Behavior, 2011, 6(7): 1024-1025.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0898300|<br /> Description = Armadillo-like helical domain containing protein|<br /> Version = NM_001051627.1 GI:115441624 GeneID:4325109|<br /> Length = 6243 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0898300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:40808134..40814376|<br /> CDS = 40808350..40808496,40808589..40808780,40808893..40809024,40809123..40809260,40809362..40809562&lt;br&gt;,40809661..40809788,40810023..40810098,40810453..40810602,40810698..40811057&lt;br&gt;,40811144..40811296,40811384..40811676,40811792..40811988,40812071..40812228&lt;br&gt;,40812533..40812580,40812912..40813040,40813123..40813237,40813321..40813436&lt;br&gt;,40813737..40813811,40813982..40814116|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaaaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaaatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgatag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MGVMSRRVLPACSSLCYFCPSLRARSRQPVKRYKKIIAEIYQLP PDGEPNDRRIGKLCDYVSRNPTRIPKITEYLEERCYKDLRHENFTLAKVVPCIYRKLL CSCKDHTPLLATSTLSIIRTLLDQRMNDDLRVLGCLMLVDFLNGQVDSTHMFNLEGLI PKLCQISQELREDDKGFRLRCAALQALASMVQYMGDHSHISMELDEVVSVIVSCYEVN QTLSIKEVVRLQDDDDLVINGSLTGLPVSGQNSAKVASDTMSASENPAHWARVCLRNM ASIAKEATTVWRVLDPLFRLFDSHNYWSPENGIAFSILQEMQALMDKSGQNGHLLLSF TIKHIDHKSVAKKPAKQTSILKVASLLAKHAKLKASVTIASATSDLIKHLRKCMHCAV ESPNAQNDVDKWNSALYVALEECLVQLTEKVGDVGPVLDMVGVMLENLSCTATIARTT ISSVFRTVQIAASIHKSLYNQKAFPEALFHQLLLAMMHPDKKTRVGSHRVLSTIIAPS LLCPWSGISFPIPVKGNDSQSITLLALSAFSSEAVMDEVRIKSRTHEQLQNNVKPETV VGSENGYTHTEPNSRKSPGLGIPLKDENLKFMKLNSSQLVLLLSSIWSQAPLEDNSPA NFEAMCHTYNIALLCSMTKSSSHAALVRCFQLAFSLRRMSLNQENGLQPSRRRCLYTM ASAMLIFSAKVADIPQTIPLVKAAVPEKMVDPHLCLIDDCRLVISSPQSSNSGIVYGS EEDESDARNFLSCVNKNDTQLKEIVISHFKEKFENLSEKFNGIEEQLLQEFSLDDSFP LSAPLFMETPHSCSMYAEKDDHCFDEEVIPCEMDDDDDIVFEHSGSQSDRKTSGSMAS SDVLNVNQLIESVHETARQVANAPVSANLVPYDQMKSQCEALVMEKQQKMSVLLSFKH SRTDSRGSTAENGLETNESSARSEPETQSTRKERMRRSDSASSESDRSFRLPPASPYD KFMRAAGR&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5881..6027#5597..5788#5353..5484#5117..5254#4815..5015#4589..4716#4279..4354#3775..3924#3320..3679#3081..3233#2701..2993#2389..2585#2149..2306#1797..1844#1337..1465#1140..1254#941..1056#566..640#261..395#gaggtttgatcttggtttcgatccgaagggtctcggttggctgcgtgggaagggggaggaggaagaaggcgacctgctatctaatcctctaccccctctgctgcgaaaattctctgctgtttgctcaagcatcttgctgcttggagccttggatcgaagagagttttagttcttggagttctgagaattgtgttgcttgagccgaattcgatcccaaaaaggctcttttttgggaggctttgaggaggaggaggaagaagatgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggtatggcgattactgaatcctgtcttggcatttcagccatttctgatgcttagttgtagtctctgcgatttgggtctgtggtcaaatagttctagtttctgcgaattggagtctgtggtcaaatgtgtggagggagtttgttgattgagggtgtgatgttgttgctgtaggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaaggtaacacattttgatctcctctctgtttttactagaattgtcgcgcaatctgttagcttgaatagtttagactgaatttccagtatttctgaaagcagtctttataggtaccgagtagttaccacataatggctgatttacactgcattgctttagtgtgatggtgattccttttgccagagtacaaccgatcaactttcgctagcaccgatcactagctggaatgattttaggctgtttaagtaggacttaccgagatgaatgaatttgtggaactcggaactgattttatgtctgcagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacgtaagtgtaaaggctctattctgccagtcaagcacccatagcaaatgtttgctgctgtactgaccatattattcttgattcagaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttattgacgactgtaacaaattgcacctacttggtttgctagaaatctgccatctgtttggtttaatttgagttcatgtaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtaagaaaagtttttacttgattctctgatgaaatggataaggtagtcttatatgttgcgagctagccatttagggctaatttacagtagatttgttggaccagaaataataatgtagaacaaaataaggaactacattcaactattatacaatttgttttgcaaatataacatttttttaatgttggttatattgatattgcttgagaattcccaacagataatcctatacttgcattactggttcttgccaactgatgccattaaatcagtctattgattaaatttaactttttaatcctaatatactcatttaccactttgtaatcaggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagtaagtccttatcactctgtgttgggctagaatgttaagagttaagacttaattctcattcatggttgatagatgcctcctggtgaatatttgttctcttgttggaattgcataagattcatctttggagcatagacaagttgatgccattaatactacagatagacttgttgaataagtggaagatatttgattagtctatgaatagaaaaaaaaagtagcatgatagtttcatttgaagtgcgccagaaacagtaattcagtaagggttctgattagtctctgaattcattcttgaacccaggttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtgagaatttctgctaatgtaacggcccatttccacactcgatttgtgcccttgtcatcttaatgacttagtgtgttgacaggtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcaggtgcaaaatttttcacccatgctatttgtttctctttttgtgccattttttttggttttgttagtgtttaacagcagaaagatttagtgctcttctaaatcttgtttcttaacagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttcattatgctgtattattcctttgttacattactacattttgctttcatcagagagacattaaaggctgttaattcattgaataggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcaagttttctagtgtgttcaccattgcaggagaattttgctattgaacaaaagtgttaattggcaattccttgttaatatttcaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaagtatgtactccaactagtattcttattgaacttggtattttctggtcttggtgttttcggatgtccagctgacacattttcttccactgaagcagaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaaggtaggtcacttaatctagttcattcttgttatacaataggcaaaaagaattgttcaagtaacttgacttaacattgttctgcatgccttgaaattatcaaagaatagccaaagggaaagataccgtcttttcccttttggcatggagttatttcatattatataatatttttttatactctatttttacacaagatagattctttagaatattttattgatgtaataggcatggattaacactttttttaagatttccctgctagtgtgagtttgactagtgatgtcaacttctcctttggacttgaaattgtgtgctatatttgtgagattgaccattgaattttcgttgcagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaagtaagtttatcctcatttgcctgctctttaaagtttttgcttttgtatatcaaaatgatgatgcaaaaaaaaagtgacaactagctagggattttttccaaataggaatatcattttttctaataccagtatattttagccattgcgtccaacttcaagtttattatatttcctatcaatctagtcatttgatacctgcttgatttattcttacattttctactgtaaataaagatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggttagttgattcctgattttgaatatgcttgctctatatatatctctaatgtgacatttttcaatttactctgaattcgcttattgatgtttttgtaggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagaggtatacattcagatttacattatgagagcgtgttttcttgatttctctgttcacaatcaagaagttatttgacgattaagtgtcttcactgcaaattgcagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggtccgtactgattctctatagttaggtgattttttgttatttatgaattgctgttgtattaacttttttggttgcatctgcgtcccttgtgcttccaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggtatgcgccatttctgtctacaacaatggcacatgaagatcactcgctattttctagatctcttacagtcccctcatccgtttgtaatcaaacgacttttttttttcttcaggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgaggcatgttcatcttgacaggaaaaaaaaaatctatccatcactactgaaatctttgaaaatgtcctgaaaaactcatcaacatcttgttgcagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgataggctggtgcaatgcaatgcgatggcgccaaggatctgatactagttttcaagcctgtatacagaaatgtttttacagatacaagtcttctaaagctgtttcttttcattgtcgccttgtattttttattcttctgagtgtaaatttctcgagaaggtgtacaaatttctgttactgttgtagagacccagaataaataaaagtataggcattggtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051627.1 RefSeq:Os01g0898300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0898300&diff=176663 2014-06-03T10:45:20Z

<p>Wuxt: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> OsSPR1 was identified by map-based cloning. It encodes a novel mitochondrial protein with the Armadillo-like repeat domain. Osspr1 mutants exhibited decreased root cell elongation. The large reduction of Fe content observed in the leaves but not the roots of Osspr1 mutant plants under Fe(II) and Fe(III) nutrient supply, and the normal Fe deficiency response for both strategy I and II marker genes in the leaves but not the roots indicate a defect in Fe homeostasis. In addition, root development was also compromised in Osspr1 mutant plants. It is likely that the defective roots of Osspr1 plants will have a secondary effect on nutrient uptake. However, if reduced Fe uptake as a result of damaged roots was the primary reason for the reduced Fe content in the leaves, the Fe content and the Fe deficiency response would have been duced and activated, respectively, in the roots. However, the Fe content in roots of Osspr1 mutant plants was similar to that of wild-type plants (Fig. 6e). Also, no Fe deficiency response was observed in roots (Fig. 7). However, a lower Fe content was observed in the mutant leaves, which was inconsistent with the induction of Fe-responsive genes (Figs 6c, 7). These results indicate that OsSPR1 plays a role in maintaining Fe (and other metal) homeostasis between roots and leaves.<br /> <br /> <br /> [[File:Fig.7.jpg]]<br /> [[File:Fig.6c.jpg]]<br /> [[File:Fig.6e.jpg]]<br /> <br /> ===Expression===<br /> <br /> The expression pattern of OsSPR1 was determined usingt he GUS gene driven by the OsSPR1 promoter transferred into wild-type plants. GUS staining in transgenic plants indicated that OsSPR1 was ubiquitously expressed in all plant organs, including the root, leaf, stem and spikelet (Fig. 3a–r). OsSPR1 was expressed in primary and lateral roots, both in mature regions and in root tips. Strong expression was observed in the root tip (in the meristem and lateral root initiation regions), where dividing cells are present. The expression of OsSPR1 in the root is mainly associated with vascular tissues (Fig. 3b,d,h). Semiquantitative RT-PCR analysis using SPR1-specific primers showed that SPR1 was expressed at relatively similar levelsin all tissues, including the root, stem base, stem, leaf and panicles (Fig. 3s), consistent with the GUS staining patterns observed.<br /> <br /> ===Evolution===<br /> Root elongation is determined by two successive processes: cell elongation and cell proliferation (Beemster et al., 2003). In rice, several genes controlling division and differentiation in root growth and development have been identified through genetic screens (Rebouillat et al., 2009). Here, we cloned OsSPR1, a novel mitochondrial gene involved in root cell elongation. Loss of function of OsSPR1 resulted in defective root elongation, including primary root, adventitious root and lateral root elongation; notably, the defects were more severe in lateral roots and adventitious roots. Analysis of the different root zones revealed similar arrangements of cells in those zones compared with the wild type, but reduced cell length was observed in the spr1 roots,<br /> suggesting that reduced root length in spr1 resulted from reduced cell elongation.<br /> ==Labs working on this gene==<br /> *Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Ghent University, Belgium.<br /> *State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China <br /> *ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, 6009 WA, Australia<br /> *Joint Laboratory in Genomics and Nutriomics, College of Life Science, Zhejiang University, Hangzhou 310058, China<br /> *Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China<br /> <br /> ==References==<br /> 1.Beemster GTS, Fiorani F, Inze´ D. 2003. Cell cycle: the key to plant growth control? Trends in Plant Science 8: 154–158.<br /> <br /> 2.Rebouillat J, Dievart A, Verdeil J, Escoute J, Giese G, Breitler J, Gantet P, Espeout S, Guiderdoni E, Pe´rin C. 2009. Molecular genetics of rice root development. Rice 2: 15–34.<br /> <br /> 3.Liqiang Jia;Zhongchang Wu;Xi Hao;Chris Carrie;Libin Zheng;James Whelan;Yunrong Wu;Shoufeng Wang;Ping Wu;Chuanzao Mao. Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa.New Phytologist, 2011, 189(3): 843-855.<br /> <br /> 4.Jia LiQiang . Is Reactive Oxygen Species (ROS) the underlying factor for inhibited root growth in Osspr1? Plant Signaling &amp; Behavior, 2011, 6(7): 1024-1025.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0898300|<br /> Description = Armadillo-like helical domain containing protein|<br /> Version = NM_001051627.1 GI:115441624 GeneID:4325109|<br /> Length = 6243 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0898300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:40808134..40814376|<br /> CDS = 40808350..40808496,40808589..40808780,40808893..40809024,40809123..40809260,40809362..40809562&lt;br&gt;,40809661..40809788,40810023..40810098,40810453..40810602,40810698..40811057&lt;br&gt;,40811144..40811296,40811384..40811676,40811792..40811988,40812071..40812228&lt;br&gt;,40812533..40812580,40812912..40813040,40813123..40813237,40813321..40813436&lt;br&gt;,40813737..40813811,40813982..40814116|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaaaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaaatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgatag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MGVMSRRVLPACSSLCYFCPSLRARSRQPVKRYKKIIAEIYQLP PDGEPNDRRIGKLCDYVSRNPTRIPKITEYLEERCYKDLRHENFTLAKVVPCIYRKLL CSCKDHTPLLATSTLSIIRTLLDQRMNDDLRVLGCLMLVDFLNGQVDSTHMFNLEGLI PKLCQISQELREDDKGFRLRCAALQALASMVQYMGDHSHISMELDEVVSVIVSCYEVN QTLSIKEVVRLQDDDDLVINGSLTGLPVSGQNSAKVASDTMSASENPAHWARVCLRNM ASIAKEATTVWRVLDPLFRLFDSHNYWSPENGIAFSILQEMQALMDKSGQNGHLLLSF TIKHIDHKSVAKKPAKQTSILKVASLLAKHAKLKASVTIASATSDLIKHLRKCMHCAV ESPNAQNDVDKWNSALYVALEECLVQLTEKVGDVGPVLDMVGVMLENLSCTATIARTT ISSVFRTVQIAASIHKSLYNQKAFPEALFHQLLLAMMHPDKKTRVGSHRVLSTIIAPS LLCPWSGISFPIPVKGNDSQSITLLALSAFSSEAVMDEVRIKSRTHEQLQNNVKPETV VGSENGYTHTEPNSRKSPGLGIPLKDENLKFMKLNSSQLVLLLSSIWSQAPLEDNSPA NFEAMCHTYNIALLCSMTKSSSHAALVRCFQLAFSLRRMSLNQENGLQPSRRRCLYTM ASAMLIFSAKVADIPQTIPLVKAAVPEKMVDPHLCLIDDCRLVISSPQSSNSGIVYGS EEDESDARNFLSCVNKNDTQLKEIVISHFKEKFENLSEKFNGIEEQLLQEFSLDDSFP LSAPLFMETPHSCSMYAEKDDHCFDEEVIPCEMDDDDDIVFEHSGSQSDRKTSGSMAS SDVLNVNQLIESVHETARQVANAPVSANLVPYDQMKSQCEALVMEKQQKMSVLLSFKH SRTDSRGSTAENGLETNESSARSEPETQSTRKERMRRSDSASSESDRSFRLPPASPYD KFMRAAGR&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5881..6027#5597..5788#5353..5484#5117..5254#4815..5015#4589..4716#4279..4354#3775..3924#3320..3679#3081..3233#2701..2993#2389..2585#2149..2306#1797..1844#1337..1465#1140..1254#941..1056#566..640#261..395#gaggtttgatcttggtttcgatccgaagggtctcggttggctgcgtgggaagggggaggaggaagaaggcgacctgctatctaatcctctaccccctctgctgcgaaaattctctgctgtttgctcaagcatcttgctgcttggagccttggatcgaagagagttttagttcttggagttctgagaattgtgttgcttgagccgaattcgatcccaaaaaggctcttttttgggaggctttgaggaggaggaggaagaagatgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggtatggcgattactgaatcctgtcttggcatttcagccatttctgatgcttagttgtagtctctgcgatttgggtctgtggtcaaatagttctagtttctgcgaattggagtctgtggtcaaatgtgtggagggagtttgttgattgagggtgtgatgttgttgctgtaggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaaggtaacacattttgatctcctctctgtttttactagaattgtcgcgcaatctgttagcttgaatagtttagactgaatttccagtatttctgaaagcagtctttataggtaccgagtagttaccacataatggctgatttacactgcattgctttagtgtgatggtgattccttttgccagagtacaaccgatcaactttcgctagcaccgatcactagctggaatgattttaggctgtttaagtaggacttaccgagatgaatgaatttgtggaactcggaactgattttatgtctgcagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacgtaagtgtaaaggctctattctgccagtcaagcacccatagcaaatgtttgctgctgtactgaccatattattcttgattcagaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttattgacgactgtaacaaattgcacctacttggtttgctagaaatctgccatctgtttggtttaatttgagttcatgtaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtaagaaaagtttttacttgattctctgatgaaatggataaggtagtcttatatgttgcgagctagccatttagggctaatttacagtagatttgttggaccagaaataataatgtagaacaaaataaggaactacattcaactattatacaatttgttttgcaaatataacatttttttaatgttggttatattgatattgcttgagaattcccaacagataatcctatacttgcattactggttcttgccaactgatgccattaaatcagtctattgattaaatttaactttttaatcctaatatactcatttaccactttgtaatcaggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagtaagtccttatcactctgtgttgggctagaatgttaagagttaagacttaattctcattcatggttgatagatgcctcctggtgaatatttgttctcttgttggaattgcataagattcatctttggagcatagacaagttgatgccattaatactacagatagacttgttgaataagtggaagatatttgattagtctatgaatagaaaaaaaaagtagcatgatagtttcatttgaagtgcgccagaaacagtaattcagtaagggttctgattagtctctgaattcattcttgaacccaggttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtgagaatttctgctaatgtaacggcccatttccacactcgatttgtgcccttgtcatcttaatgacttagtgtgttgacaggtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcaggtgcaaaatttttcacccatgctatttgtttctctttttgtgccattttttttggttttgttagtgtttaacagcagaaagatttagtgctcttctaaatcttgtttcttaacagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttcattatgctgtattattcctttgttacattactacattttgctttcatcagagagacattaaaggctgttaattcattgaataggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcaagttttctagtgtgttcaccattgcaggagaattttgctattgaacaaaagtgttaattggcaattccttgttaatatttcaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaagtatgtactccaactagtattcttattgaacttggtattttctggtcttggtgttttcggatgtccagctgacacattttcttccactgaagcagaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaaggtaggtcacttaatctagttcattcttgttatacaataggcaaaaagaattgttcaagtaacttgacttaacattgttctgcatgccttgaaattatcaaagaatagccaaagggaaagataccgtcttttcccttttggcatggagttatttcatattatataatatttttttatactctatttttacacaagatagattctttagaatattttattgatgtaataggcatggattaacactttttttaagatttccctgctagtgtgagtttgactagtgatgtcaacttctcctttggacttgaaattgtgtgctatatttgtgagattgaccattgaattttcgttgcagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaagtaagtttatcctcatttgcctgctctttaaagtttttgcttttgtatatcaaaatgatgatgcaaaaaaaaagtgacaactagctagggattttttccaaataggaatatcattttttctaataccagtatattttagccattgcgtccaacttcaagtttattatatttcctatcaatctagtcatttgatacctgcttgatttattcttacattttctactgtaaataaagatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggttagttgattcctgattttgaatatgcttgctctatatatatctctaatgtgacatttttcaatttactctgaattcgcttattgatgtttttgtaggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagaggtatacattcagatttacattatgagagcgtgttttcttgatttctctgttcacaatcaagaagttatttgacgattaagtgtcttcactgcaaattgcagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggtccgtactgattctctatagttaggtgattttttgttatttatgaattgctgttgtattaacttttttggttgcatctgcgtcccttgtgcttccaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggtatgcgccatttctgtctacaacaatggcacatgaagatcactcgctattttctagatctcttacagtcccctcatccgtttgtaatcaaacgacttttttttttcttcaggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgaggcatgttcatcttgacaggaaaaaaaaaatctatccatcactactgaaatctttgaaaatgtcctgaaaaactcatcaacatcttgttgcagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgataggctggtgcaatgcaatgcgatggcgccaaggatctgatactagttttcaagcctgtatacagaaatgtttttacagatacaagtcttctaaagctgtttcttttcattgtcgccttgtattttttattcttctgagtgtaaatttctcgagaaggtgtacaaatttctgttactgttgtagagacccagaataaataaaagtataggcattggtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051627.1 RefSeq:Os01g0898300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0898300&diff=176662 2014-06-03T10:45:01Z

<p>Wuxt: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> OsSPR1 was identified by map-based cloning. It encodes a novel mitochondrial protein with the Armadillo-like repeat domain. Osspr1 mutants exhibited decreased root cell elongation. The large reduction of Fe content observed in the leaves but not the roots of Osspr1 mutant plants under Fe(II) and Fe(III) nutrient supply, and the normal Fe deficiency response for both strategy I and II marker genes in the leaves but not the roots indicate a defect in Fe homeostasis. In addition, root development was also compromised in Osspr1 mutant plants. It is likely that the defective roots of Osspr1 plants will have a secondary effect on nutrient uptake. However, if reduced Fe uptake as a result of damaged roots was the primary reason for the reduced Fe content in the leaves, the Fe content and the Fe deficiency response would have been duced and activated, respectively, in the roots. However, the Fe content in roots of Osspr1 mutant plants was similar to that of wild-type plants (Fig. 6e). Also, no Fe deficiency response was observed in roots (Fig. 7). However, a lower Fe content was observed in the mutant leaves, which was inconsistent with the induction of Fe-responsive genes (Figs 6c, 7). These results indicate that OsSPR1 plays a role in maintaining Fe (and other metal) homeostasis between roots and leaves.<br /> <br /> <br /> [[File:Fig.7.jpg]]<br /> [[File:Fig.6c.jpg]]<br /> [[File:Fig6e.jpg]]<br /> <br /> ===Expression===<br /> <br /> The expression pattern of OsSPR1 was determined usingt he GUS gene driven by the OsSPR1 promoter transferred into wild-type plants. GUS staining in transgenic plants indicated that OsSPR1 was ubiquitously expressed in all plant organs, including the root, leaf, stem and spikelet (Fig. 3a–r). OsSPR1 was expressed in primary and lateral roots, both in mature regions and in root tips. Strong expression was observed in the root tip (in the meristem and lateral root initiation regions), where dividing cells are present. The expression of OsSPR1 in the root is mainly associated with vascular tissues (Fig. 3b,d,h). Semiquantitative RT-PCR analysis using SPR1-specific primers showed that SPR1 was expressed at relatively similar levelsin all tissues, including the root, stem base, stem, leaf and panicles (Fig. 3s), consistent with the GUS staining patterns observed.<br /> <br /> ===Evolution===<br /> Root elongation is determined by two successive processes: cell elongation and cell proliferation (Beemster et al., 2003). In rice, several genes controlling division and differentiation in root growth and development have been identified through genetic screens (Rebouillat et al., 2009). Here, we cloned OsSPR1, a novel mitochondrial gene involved in root cell elongation. Loss of function of OsSPR1 resulted in defective root elongation, including primary root, adventitious root and lateral root elongation; notably, the defects were more severe in lateral roots and adventitious roots. Analysis of the different root zones revealed similar arrangements of cells in those zones compared with the wild type, but reduced cell length was observed in the spr1 roots,<br /> suggesting that reduced root length in spr1 resulted from reduced cell elongation.<br /> ==Labs working on this gene==<br /> *Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Ghent University, Belgium.<br /> *State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China <br /> *ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, 6009 WA, Australia<br /> *Joint Laboratory in Genomics and Nutriomics, College of Life Science, Zhejiang University, Hangzhou 310058, China<br /> *Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China<br /> <br /> ==References==<br /> 1.Beemster GTS, Fiorani F, Inze´ D. 2003. Cell cycle: the key to plant growth control? Trends in Plant Science 8: 154–158.<br /> <br /> 2.Rebouillat J, Dievart A, Verdeil J, Escoute J, Giese G, Breitler J, Gantet P, Espeout S, Guiderdoni E, Pe´rin C. 2009. Molecular genetics of rice root development. Rice 2: 15–34.<br /> <br /> 3.Liqiang Jia;Zhongchang Wu;Xi Hao;Chris Carrie;Libin Zheng;James Whelan;Yunrong Wu;Shoufeng Wang;Ping Wu;Chuanzao Mao. Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa.New Phytologist, 2011, 189(3): 843-855.<br /> <br /> 4.Jia LiQiang . Is Reactive Oxygen Species (ROS) the underlying factor for inhibited root growth in Osspr1? Plant Signaling &amp; Behavior, 2011, 6(7): 1024-1025.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0898300|<br /> Description = Armadillo-like helical domain containing protein|<br /> Version = NM_001051627.1 GI:115441624 GeneID:4325109|<br /> Length = 6243 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0898300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:40808134..40814376|<br /> CDS = 40808350..40808496,40808589..40808780,40808893..40809024,40809123..40809260,40809362..40809562&lt;br&gt;,40809661..40809788,40810023..40810098,40810453..40810602,40810698..40811057&lt;br&gt;,40811144..40811296,40811384..40811676,40811792..40811988,40812071..40812228&lt;br&gt;,40812533..40812580,40812912..40813040,40813123..40813237,40813321..40813436&lt;br&gt;,40813737..40813811,40813982..40814116|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaaaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaaatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgatag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MGVMSRRVLPACSSLCYFCPSLRARSRQPVKRYKKIIAEIYQLP PDGEPNDRRIGKLCDYVSRNPTRIPKITEYLEERCYKDLRHENFTLAKVVPCIYRKLL CSCKDHTPLLATSTLSIIRTLLDQRMNDDLRVLGCLMLVDFLNGQVDSTHMFNLEGLI PKLCQISQELREDDKGFRLRCAALQALASMVQYMGDHSHISMELDEVVSVIVSCYEVN QTLSIKEVVRLQDDDDLVINGSLTGLPVSGQNSAKVASDTMSASENPAHWARVCLRNM ASIAKEATTVWRVLDPLFRLFDSHNYWSPENGIAFSILQEMQALMDKSGQNGHLLLSF TIKHIDHKSVAKKPAKQTSILKVASLLAKHAKLKASVTIASATSDLIKHLRKCMHCAV ESPNAQNDVDKWNSALYVALEECLVQLTEKVGDVGPVLDMVGVMLENLSCTATIARTT ISSVFRTVQIAASIHKSLYNQKAFPEALFHQLLLAMMHPDKKTRVGSHRVLSTIIAPS LLCPWSGISFPIPVKGNDSQSITLLALSAFSSEAVMDEVRIKSRTHEQLQNNVKPETV VGSENGYTHTEPNSRKSPGLGIPLKDENLKFMKLNSSQLVLLLSSIWSQAPLEDNSPA NFEAMCHTYNIALLCSMTKSSSHAALVRCFQLAFSLRRMSLNQENGLQPSRRRCLYTM ASAMLIFSAKVADIPQTIPLVKAAVPEKMVDPHLCLIDDCRLVISSPQSSNSGIVYGS EEDESDARNFLSCVNKNDTQLKEIVISHFKEKFENLSEKFNGIEEQLLQEFSLDDSFP LSAPLFMETPHSCSMYAEKDDHCFDEEVIPCEMDDDDDIVFEHSGSQSDRKTSGSMAS SDVLNVNQLIESVHETARQVANAPVSANLVPYDQMKSQCEALVMEKQQKMSVLLSFKH SRTDSRGSTAENGLETNESSARSEPETQSTRKERMRRSDSASSESDRSFRLPPASPYD KFMRAAGR&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5881..6027#5597..5788#5353..5484#5117..5254#4815..5015#4589..4716#4279..4354#3775..3924#3320..3679#3081..3233#2701..2993#2389..2585#2149..2306#1797..1844#1337..1465#1140..1254#941..1056#566..640#261..395#gaggtttgatcttggtttcgatccgaagggtctcggttggctgcgtgggaagggggaggaggaagaaggcgacctgctatctaatcctctaccccctctgctgcgaaaattctctgctgtttgctcaagcatcttgctgcttggagccttggatcgaagagagttttagttcttggagttctgagaattgtgttgcttgagccgaattcgatcccaaaaaggctcttttttgggaggctttgaggaggaggaggaagaagatgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggtatggcgattactgaatcctgtcttggcatttcagccatttctgatgcttagttgtagtctctgcgatttgggtctgtggtcaaatagttctagtttctgcgaattggagtctgtggtcaaatgtgtggagggagtttgttgattgagggtgtgatgttgttgctgtaggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaaggtaacacattttgatctcctctctgtttttactagaattgtcgcgcaatctgttagcttgaatagtttagactgaatttccagtatttctgaaagcagtctttataggtaccgagtagttaccacataatggctgatttacactgcattgctttagtgtgatggtgattccttttgccagagtacaaccgatcaactttcgctagcaccgatcactagctggaatgattttaggctgtttaagtaggacttaccgagatgaatgaatttgtggaactcggaactgattttatgtctgcagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacgtaagtgtaaaggctctattctgccagtcaagcacccatagcaaatgtttgctgctgtactgaccatattattcttgattcagaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttattgacgactgtaacaaattgcacctacttggtttgctagaaatctgccatctgtttggtttaatttgagttcatgtaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtaagaaaagtttttacttgattctctgatgaaatggataaggtagtcttatatgttgcgagctagccatttagggctaatttacagtagatttgttggaccagaaataataatgtagaacaaaataaggaactacattcaactattatacaatttgttttgcaaatataacatttttttaatgttggttatattgatattgcttgagaattcccaacagataatcctatacttgcattactggttcttgccaactgatgccattaaatcagtctattgattaaatttaactttttaatcctaatatactcatttaccactttgtaatcaggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagtaagtccttatcactctgtgttgggctagaatgttaagagttaagacttaattctcattcatggttgatagatgcctcctggtgaatatttgttctcttgttggaattgcataagattcatctttggagcatagacaagttgatgccattaatactacagatagacttgttgaataagtggaagatatttgattagtctatgaatagaaaaaaaaagtagcatgatagtttcatttgaagtgcgccagaaacagtaattcagtaagggttctgattagtctctgaattcattcttgaacccaggttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtgagaatttctgctaatgtaacggcccatttccacactcgatttgtgcccttgtcatcttaatgacttagtgtgttgacaggtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcaggtgcaaaatttttcacccatgctatttgtttctctttttgtgccattttttttggttttgttagtgtttaacagcagaaagatttagtgctcttctaaatcttgtttcttaacagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttcattatgctgtattattcctttgttacattactacattttgctttcatcagagagacattaaaggctgttaattcattgaataggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcaagttttctagtgtgttcaccattgcaggagaattttgctattgaacaaaagtgttaattggcaattccttgttaatatttcaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaagtatgtactccaactagtattcttattgaacttggtattttctggtcttggtgttttcggatgtccagctgacacattttcttccactgaagcagaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaaggtaggtcacttaatctagttcattcttgttatacaataggcaaaaagaattgttcaagtaacttgacttaacattgttctgcatgccttgaaattatcaaagaatagccaaagggaaagataccgtcttttcccttttggcatggagttatttcatattatataatatttttttatactctatttttacacaagatagattctttagaatattttattgatgtaataggcatggattaacactttttttaagatttccctgctagtgtgagtttgactagtgatgtcaacttctcctttggacttgaaattgtgtgctatatttgtgagattgaccattgaattttcgttgcagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaagtaagtttatcctcatttgcctgctctttaaagtttttgcttttgtatatcaaaatgatgatgcaaaaaaaaagtgacaactagctagggattttttccaaataggaatatcattttttctaataccagtatattttagccattgcgtccaacttcaagtttattatatttcctatcaatctagtcatttgatacctgcttgatttattcttacattttctactgtaaataaagatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggttagttgattcctgattttgaatatgcttgctctatatatatctctaatgtgacatttttcaatttactctgaattcgcttattgatgtttttgtaggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagaggtatacattcagatttacattatgagagcgtgttttcttgatttctctgttcacaatcaagaagttatttgacgattaagtgtcttcactgcaaattgcagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggtccgtactgattctctatagttaggtgattttttgttatttatgaattgctgttgtattaacttttttggttgcatctgcgtcccttgtgcttccaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggtatgcgccatttctgtctacaacaatggcacatgaagatcactcgctattttctagatctcttacagtcccctcatccgtttgtaatcaaacgacttttttttttcttcaggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgaggcatgttcatcttgacaggaaaaaaaaaatctatccatcactactgaaatctttgaaaatgtcctgaaaaactcatcaacatcttgttgcagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgataggctggtgcaatgcaatgcgatggcgccaaggatctgatactagttttcaagcctgtatacagaaatgtttttacagatacaagtcttctaaagctgtttcttttcattgtcgccttgtattttttattcttctgagtgtaaatttctcgagaaggtgtacaaatttctgttactgttgtagagacccagaataaataaaagtataggcattggtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051627.1 RefSeq:Os01g0898300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0898300&diff=176661 2014-06-03T10:42:53Z

<p>Wuxt: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> OsSPR1 was identified by map-based cloning. It encodes a novel mitochondrial protein with the Armadillo-like repeat domain. Osspr1 mutants exhibited decreased root cell elongation. The large reduction of Fe content observed in the leaves but not the roots of Osspr1 mutant plants under Fe(II) and Fe(III) nutrient supply, and the normal Fe deficiency response for both strategy I and II marker genes in the leaves but not the roots indicate a defect in Fe homeostasis. In addition, root development was also compromised in Osspr1 mutant plants. It is likely that the defective roots of Osspr1 plants will have a secondary effect on nutrient uptake. However, if reduced Fe uptake as a result of damaged roots was the primary reason for the reduced Fe content in the leaves, the Fe content and the Fe deficiency response would have been duced and activated, respectively, in the roots. However, the Fe content in roots of Osspr1 mutant plants was similar to that of wild-type plants (Fig. 6e). Also, no Fe deficiency response was observed in roots (Fig. 7). However, a lower Fe content was observed in the mutant leaves, which was inconsistent with the induction of Fe-responsive genes (Figs 6c, 7). These results indicate that OsSPR1 plays a role in maintaining Fe (and other metal) homeostasis between roots and leaves.<br /> <br /> <br /> [[File:Fig.7.jpg]]<br /> <br /> ===Expression===<br /> <br /> The expression pattern of OsSPR1 was determined usingt he GUS gene driven by the OsSPR1 promoter transferred into wild-type plants. GUS staining in transgenic plants indicated that OsSPR1 was ubiquitously expressed in all plant organs, including the root, leaf, stem and spikelet (Fig. 3a–r). OsSPR1 was expressed in primary and lateral roots, both in mature regions and in root tips. Strong expression was observed in the root tip (in the meristem and lateral root initiation regions), where dividing cells are present. The expression of OsSPR1 in the root is mainly associated with vascular tissues (Fig. 3b,d,h). Semiquantitative RT-PCR analysis using SPR1-specific primers showed that SPR1 was expressed at relatively similar levelsin all tissues, including the root, stem base, stem, leaf and panicles (Fig. 3s), consistent with the GUS staining patterns observed.<br /> <br /> ===Evolution===<br /> Root elongation is determined by two successive processes: cell elongation and cell proliferation (Beemster et al., 2003). In rice, several genes controlling division and differentiation in root growth and development have been identified through genetic screens (Rebouillat et al., 2009). Here, we cloned OsSPR1, a novel mitochondrial gene involved in root cell elongation. Loss of function of OsSPR1 resulted in defective root elongation, including primary root, adventitious root and lateral root elongation; notably, the defects were more severe in lateral roots and adventitious roots. Analysis of the different root zones revealed similar arrangements of cells in those zones compared with the wild type, but reduced cell length was observed in the spr1 roots,<br /> suggesting that reduced root length in spr1 resulted from reduced cell elongation.<br /> ==Labs working on this gene==<br /> *Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Ghent University, Belgium.<br /> *State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China <br /> *ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, 6009 WA, Australia<br /> *Joint Laboratory in Genomics and Nutriomics, College of Life Science, Zhejiang University, Hangzhou 310058, China<br /> *Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China<br /> <br /> ==References==<br /> 1.Beemster GTS, Fiorani F, Inze´ D. 2003. Cell cycle: the key to plant growth control? Trends in Plant Science 8: 154–158.<br /> <br /> 2.Rebouillat J, Dievart A, Verdeil J, Escoute J, Giese G, Breitler J, Gantet P, Espeout S, Guiderdoni E, Pe´rin C. 2009. Molecular genetics of rice root development. Rice 2: 15–34.<br /> <br /> 3.Liqiang Jia;Zhongchang Wu;Xi Hao;Chris Carrie;Libin Zheng;James Whelan;Yunrong Wu;Shoufeng Wang;Ping Wu;Chuanzao Mao. Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa.New Phytologist, 2011, 189(3): 843-855.<br /> <br /> 4.Jia LiQiang . Is Reactive Oxygen Species (ROS) the underlying factor for inhibited root growth in Osspr1? Plant Signaling &amp; Behavior, 2011, 6(7): 1024-1025.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0898300|<br /> Description = Armadillo-like helical domain containing protein|<br /> Version = NM_001051627.1 GI:115441624 GeneID:4325109|<br /> Length = 6243 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0898300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:40808134..40814376|<br /> CDS = 40808350..40808496,40808589..40808780,40808893..40809024,40809123..40809260,40809362..40809562&lt;br&gt;,40809661..40809788,40810023..40810098,40810453..40810602,40810698..40811057&lt;br&gt;,40811144..40811296,40811384..40811676,40811792..40811988,40812071..40812228&lt;br&gt;,40812533..40812580,40812912..40813040,40813123..40813237,40813321..40813436&lt;br&gt;,40813737..40813811,40813982..40814116|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaaaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaaatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgatag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MGVMSRRVLPACSSLCYFCPSLRARSRQPVKRYKKIIAEIYQLP PDGEPNDRRIGKLCDYVSRNPTRIPKITEYLEERCYKDLRHENFTLAKVVPCIYRKLL CSCKDHTPLLATSTLSIIRTLLDQRMNDDLRVLGCLMLVDFLNGQVDSTHMFNLEGLI PKLCQISQELREDDKGFRLRCAALQALASMVQYMGDHSHISMELDEVVSVIVSCYEVN QTLSIKEVVRLQDDDDLVINGSLTGLPVSGQNSAKVASDTMSASENPAHWARVCLRNM ASIAKEATTVWRVLDPLFRLFDSHNYWSPENGIAFSILQEMQALMDKSGQNGHLLLSF TIKHIDHKSVAKKPAKQTSILKVASLLAKHAKLKASVTIASATSDLIKHLRKCMHCAV ESPNAQNDVDKWNSALYVALEECLVQLTEKVGDVGPVLDMVGVMLENLSCTATIARTT ISSVFRTVQIAASIHKSLYNQKAFPEALFHQLLLAMMHPDKKTRVGSHRVLSTIIAPS LLCPWSGISFPIPVKGNDSQSITLLALSAFSSEAVMDEVRIKSRTHEQLQNNVKPETV VGSENGYTHTEPNSRKSPGLGIPLKDENLKFMKLNSSQLVLLLSSIWSQAPLEDNSPA NFEAMCHTYNIALLCSMTKSSSHAALVRCFQLAFSLRRMSLNQENGLQPSRRRCLYTM ASAMLIFSAKVADIPQTIPLVKAAVPEKMVDPHLCLIDDCRLVISSPQSSNSGIVYGS EEDESDARNFLSCVNKNDTQLKEIVISHFKEKFENLSEKFNGIEEQLLQEFSLDDSFP LSAPLFMETPHSCSMYAEKDDHCFDEEVIPCEMDDDDDIVFEHSGSQSDRKTSGSMAS SDVLNVNQLIESVHETARQVANAPVSANLVPYDQMKSQCEALVMEKQQKMSVLLSFKH SRTDSRGSTAENGLETNESSARSEPETQSTRKERMRRSDSASSESDRSFRLPPASPYD KFMRAAGR&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5881..6027#5597..5788#5353..5484#5117..5254#4815..5015#4589..4716#4279..4354#3775..3924#3320..3679#3081..3233#2701..2993#2389..2585#2149..2306#1797..1844#1337..1465#1140..1254#941..1056#566..640#261..395#gaggtttgatcttggtttcgatccgaagggtctcggttggctgcgtgggaagggggaggaggaagaaggcgacctgctatctaatcctctaccccctctgctgcgaaaattctctgctgtttgctcaagcatcttgctgcttggagccttggatcgaagagagttttagttcttggagttctgagaattgtgttgcttgagccgaattcgatcccaaaaaggctcttttttgggaggctttgaggaggaggaggaagaagatgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggtatggcgattactgaatcctgtcttggcatttcagccatttctgatgcttagttgtagtctctgcgatttgggtctgtggtcaaatagttctagtttctgcgaattggagtctgtggtcaaatgtgtggagggagtttgttgattgagggtgtgatgttgttgctgtaggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaaggtaacacattttgatctcctctctgtttttactagaattgtcgcgcaatctgttagcttgaatagtttagactgaatttccagtatttctgaaagcagtctttataggtaccgagtagttaccacataatggctgatttacactgcattgctttagtgtgatggtgattccttttgccagagtacaaccgatcaactttcgctagcaccgatcactagctggaatgattttaggctgtttaagtaggacttaccgagatgaatgaatttgtggaactcggaactgattttatgtctgcagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacgtaagtgtaaaggctctattctgccagtcaagcacccatagcaaatgtttgctgctgtactgaccatattattcttgattcagaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttattgacgactgtaacaaattgcacctacttggtttgctagaaatctgccatctgtttggtttaatttgagttcatgtaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtaagaaaagtttttacttgattctctgatgaaatggataaggtagtcttatatgttgcgagctagccatttagggctaatttacagtagatttgttggaccagaaataataatgtagaacaaaataaggaactacattcaactattatacaatttgttttgcaaatataacatttttttaatgttggttatattgatattgcttgagaattcccaacagataatcctatacttgcattactggttcttgccaactgatgccattaaatcagtctattgattaaatttaactttttaatcctaatatactcatttaccactttgtaatcaggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagtaagtccttatcactctgtgttgggctagaatgttaagagttaagacttaattctcattcatggttgatagatgcctcctggtgaatatttgttctcttgttggaattgcataagattcatctttggagcatagacaagttgatgccattaatactacagatagacttgttgaataagtggaagatatttgattagtctatgaatagaaaaaaaaagtagcatgatagtttcatttgaagtgcgccagaaacagtaattcagtaagggttctgattagtctctgaattcattcttgaacccaggttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtgagaatttctgctaatgtaacggcccatttccacactcgatttgtgcccttgtcatcttaatgacttagtgtgttgacaggtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcaggtgcaaaatttttcacccatgctatttgtttctctttttgtgccattttttttggttttgttagtgtttaacagcagaaagatttagtgctcttctaaatcttgtttcttaacagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttcattatgctgtattattcctttgttacattactacattttgctttcatcagagagacattaaaggctgttaattcattgaataggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcaagttttctagtgtgttcaccattgcaggagaattttgctattgaacaaaagtgttaattggcaattccttgttaatatttcaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaagtatgtactccaactagtattcttattgaacttggtattttctggtcttggtgttttcggatgtccagctgacacattttcttccactgaagcagaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaaggtaggtcacttaatctagttcattcttgttatacaataggcaaaaagaattgttcaagtaacttgacttaacattgttctgcatgccttgaaattatcaaagaatagccaaagggaaagataccgtcttttcccttttggcatggagttatttcatattatataatatttttttatactctatttttacacaagatagattctttagaatattttattgatgtaataggcatggattaacactttttttaagatttccctgctagtgtgagtttgactagtgatgtcaacttctcctttggacttgaaattgtgtgctatatttgtgagattgaccattgaattttcgttgcagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaagtaagtttatcctcatttgcctgctctttaaagtttttgcttttgtatatcaaaatgatgatgcaaaaaaaaagtgacaactagctagggattttttccaaataggaatatcattttttctaataccagtatattttagccattgcgtccaacttcaagtttattatatttcctatcaatctagtcatttgatacctgcttgatttattcttacattttctactgtaaataaagatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggttagttgattcctgattttgaatatgcttgctctatatatatctctaatgtgacatttttcaatttactctgaattcgcttattgatgtttttgtaggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagaggtatacattcagatttacattatgagagcgtgttttcttgatttctctgttcacaatcaagaagttatttgacgattaagtgtcttcactgcaaattgcagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggtccgtactgattctctatagttaggtgattttttgttatttatgaattgctgttgtattaacttttttggttgcatctgcgtcccttgtgcttccaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggtatgcgccatttctgtctacaacaatggcacatgaagatcactcgctattttctagatctcttacagtcccctcatccgtttgtaatcaaacgacttttttttttcttcaggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgaggcatgttcatcttgacaggaaaaaaaaaatctatccatcactactgaaatctttgaaaatgtcctgaaaaactcatcaacatcttgttgcagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgataggctggtgcaatgcaatgcgatggcgccaaggatctgatactagttttcaagcctgtatacagaaatgtttttacagatacaagtcttctaaagctgtttcttttcattgtcgccttgtattttttattcttctgagtgtaaatttctcgagaaggtgtacaaatttctgttactgttgtagagacccagaataaataaaagtataggcattggtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051627.1 RefSeq:Os01g0898300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0898300&diff=176660 2014-06-03T10:42:24Z

<p>Wuxt: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> OsSPR1 was identified by map-based cloning. It encodes a novel mitochondrial protein with the Armadillo-like repeat domain. Osspr1 mutants exhibited decreased root cell elongation. The large reduction of Fe content observed in the leaves but not the roots of Osspr1 mutant plants under Fe(II) and Fe(III) nutrient supply, and the normal Fe deficiency response for both strategy I and II marker genes in the leaves but not the roots indicate a defect in Fe homeostasis. In addition, root development was also compromised in Osspr1 mutant plants. It is likely that the defective roots of Osspr1 plants will have a secondary effect on nutrient uptake. However, if reduced Fe uptake as a result of damaged roots was the primary reason for the reduced Fe content in the leaves, the Fe content and the Fe deficiency response would have been duced and activated, respectively, in the roots. However, the Fe content in roots of Osspr1 mutant plants was similar to that of wild-type plants (Fig. 6e). Also, no Fe deficiency response was observed in roots (Fig. 7). However, a lower Fe content was observed in the mutant leaves, which was inconsistent with the induction of Fe-responsive genes (Figs 6c, 7). These results indicate that OsSPR1 plays a role in maintaining Fe (and other metal) homeostasis between roots and leaves.<br /> [[File:Fig.7.jpg]]<br /> <br /> ===Expression===<br /> <br /> The expression pattern of OsSPR1 was determined usingt he GUS gene driven by the OsSPR1 promoter transferred into wild-type plants. GUS staining in transgenic plants indicated that OsSPR1 was ubiquitously expressed in all plant organs, including the root, leaf, stem and spikelet (Fig. 3a–r). OsSPR1 was expressed in primary and lateral roots, both in mature regions and in root tips. Strong expression was observed in the root tip (in the meristem and lateral root initiation regions), where dividing cells are present. The expression of OsSPR1 in the root is mainly associated with vascular tissues (Fig. 3b,d,h). Semiquantitative RT-PCR analysis using SPR1-specific primers showed that SPR1 was expressed at relatively similar levelsin all tissues, including the root, stem base, stem, leaf and panicles (Fig. 3s), consistent with the GUS staining patterns observed.<br /> <br /> ===Evolution===<br /> Root elongation is determined by two successive processes: cell elongation and cell proliferation (Beemster et al., 2003). In rice, several genes controlling division and differentiation in root growth and development have been identified through genetic screens (Rebouillat et al., 2009). Here, we cloned OsSPR1, a novel mitochondrial gene involved in root cell elongation. Loss of function of OsSPR1 resulted in defective root elongation, including primary root, adventitious root and lateral root elongation; notably, the defects were more severe in lateral roots and adventitious roots. Analysis of the different root zones revealed similar arrangements of cells in those zones compared with the wild type, but reduced cell length was observed in the spr1 roots,<br /> suggesting that reduced root length in spr1 resulted from reduced cell elongation.<br /> ==Labs working on this gene==<br /> *Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Ghent University, Belgium.<br /> *State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China <br /> *ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, 6009 WA, Australia<br /> *Joint Laboratory in Genomics and Nutriomics, College of Life Science, Zhejiang University, Hangzhou 310058, China<br /> *Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China<br /> <br /> ==References==<br /> 1.Beemster GTS, Fiorani F, Inze´ D. 2003. Cell cycle: the key to plant growth control? Trends in Plant Science 8: 154–158.<br /> <br /> 2.Rebouillat J, Dievart A, Verdeil J, Escoute J, Giese G, Breitler J, Gantet P, Espeout S, Guiderdoni E, Pe´rin C. 2009. Molecular genetics of rice root development. Rice 2: 15–34.<br /> <br /> 3.Liqiang Jia;Zhongchang Wu;Xi Hao;Chris Carrie;Libin Zheng;James Whelan;Yunrong Wu;Shoufeng Wang;Ping Wu;Chuanzao Mao. Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa.New Phytologist, 2011, 189(3): 843-855.<br /> <br /> 4.Jia LiQiang . Is Reactive Oxygen Species (ROS) the underlying factor for inhibited root growth in Osspr1? Plant Signaling &amp; Behavior, 2011, 6(7): 1024-1025.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0898300|<br /> Description = Armadillo-like helical domain containing protein|<br /> Version = NM_001051627.1 GI:115441624 GeneID:4325109|<br /> Length = 6243 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0898300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:40808134..40814376|<br /> CDS = 40808350..40808496,40808589..40808780,40808893..40809024,40809123..40809260,40809362..40809562&lt;br&gt;,40809661..40809788,40810023..40810098,40810453..40810602,40810698..40811057&lt;br&gt;,40811144..40811296,40811384..40811676,40811792..40811988,40812071..40812228&lt;br&gt;,40812533..40812580,40812912..40813040,40813123..40813237,40813321..40813436&lt;br&gt;,40813737..40813811,40813982..40814116|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaaaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaaatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgatag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MGVMSRRVLPACSSLCYFCPSLRARSRQPVKRYKKIIAEIYQLP PDGEPNDRRIGKLCDYVSRNPTRIPKITEYLEERCYKDLRHENFTLAKVVPCIYRKLL CSCKDHTPLLATSTLSIIRTLLDQRMNDDLRVLGCLMLVDFLNGQVDSTHMFNLEGLI PKLCQISQELREDDKGFRLRCAALQALASMVQYMGDHSHISMELDEVVSVIVSCYEVN QTLSIKEVVRLQDDDDLVINGSLTGLPVSGQNSAKVASDTMSASENPAHWARVCLRNM ASIAKEATTVWRVLDPLFRLFDSHNYWSPENGIAFSILQEMQALMDKSGQNGHLLLSF TIKHIDHKSVAKKPAKQTSILKVASLLAKHAKLKASVTIASATSDLIKHLRKCMHCAV ESPNAQNDVDKWNSALYVALEECLVQLTEKVGDVGPVLDMVGVMLENLSCTATIARTT ISSVFRTVQIAASIHKSLYNQKAFPEALFHQLLLAMMHPDKKTRVGSHRVLSTIIAPS LLCPWSGISFPIPVKGNDSQSITLLALSAFSSEAVMDEVRIKSRTHEQLQNNVKPETV VGSENGYTHTEPNSRKSPGLGIPLKDENLKFMKLNSSQLVLLLSSIWSQAPLEDNSPA NFEAMCHTYNIALLCSMTKSSSHAALVRCFQLAFSLRRMSLNQENGLQPSRRRCLYTM ASAMLIFSAKVADIPQTIPLVKAAVPEKMVDPHLCLIDDCRLVISSPQSSNSGIVYGS EEDESDARNFLSCVNKNDTQLKEIVISHFKEKFENLSEKFNGIEEQLLQEFSLDDSFP LSAPLFMETPHSCSMYAEKDDHCFDEEVIPCEMDDDDDIVFEHSGSQSDRKTSGSMAS SDVLNVNQLIESVHETARQVANAPVSANLVPYDQMKSQCEALVMEKQQKMSVLLSFKH SRTDSRGSTAENGLETNESSARSEPETQSTRKERMRRSDSASSESDRSFRLPPASPYD KFMRAAGR&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5881..6027#5597..5788#5353..5484#5117..5254#4815..5015#4589..4716#4279..4354#3775..3924#3320..3679#3081..3233#2701..2993#2389..2585#2149..2306#1797..1844#1337..1465#1140..1254#941..1056#566..640#261..395#gaggtttgatcttggtttcgatccgaagggtctcggttggctgcgtgggaagggggaggaggaagaaggcgacctgctatctaatcctctaccccctctgctgcgaaaattctctgctgtttgctcaagcatcttgctgcttggagccttggatcgaagagagttttagttcttggagttctgagaattgtgttgcttgagccgaattcgatcccaaaaaggctcttttttgggaggctttgaggaggaggaggaagaagatgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggtatggcgattactgaatcctgtcttggcatttcagccatttctgatgcttagttgtagtctctgcgatttgggtctgtggtcaaatagttctagtttctgcgaattggagtctgtggtcaaatgtgtggagggagtttgttgattgagggtgtgatgttgttgctgtaggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaaggtaacacattttgatctcctctctgtttttactagaattgtcgcgcaatctgttagcttgaatagtttagactgaatttccagtatttctgaaagcagtctttataggtaccgagtagttaccacataatggctgatttacactgcattgctttagtgtgatggtgattccttttgccagagtacaaccgatcaactttcgctagcaccgatcactagctggaatgattttaggctgtttaagtaggacttaccgagatgaatgaatttgtggaactcggaactgattttatgtctgcagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacgtaagtgtaaaggctctattctgccagtcaagcacccatagcaaatgtttgctgctgtactgaccatattattcttgattcagaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttattgacgactgtaacaaattgcacctacttggtttgctagaaatctgccatctgtttggtttaatttgagttcatgtaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtaagaaaagtttttacttgattctctgatgaaatggataaggtagtcttatatgttgcgagctagccatttagggctaatttacagtagatttgttggaccagaaataataatgtagaacaaaataaggaactacattcaactattatacaatttgttttgcaaatataacatttttttaatgttggttatattgatattgcttgagaattcccaacagataatcctatacttgcattactggttcttgccaactgatgccattaaatcagtctattgattaaatttaactttttaatcctaatatactcatttaccactttgtaatcaggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagtaagtccttatcactctgtgttgggctagaatgttaagagttaagacttaattctcattcatggttgatagatgcctcctggtgaatatttgttctcttgttggaattgcataagattcatctttggagcatagacaagttgatgccattaatactacagatagacttgttgaataagtggaagatatttgattagtctatgaatagaaaaaaaaagtagcatgatagtttcatttgaagtgcgccagaaacagtaattcagtaagggttctgattagtctctgaattcattcttgaacccaggttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtgagaatttctgctaatgtaacggcccatttccacactcgatttgtgcccttgtcatcttaatgacttagtgtgttgacaggtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcaggtgcaaaatttttcacccatgctatttgtttctctttttgtgccattttttttggttttgttagtgtttaacagcagaaagatttagtgctcttctaaatcttgtttcttaacagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttcattatgctgtattattcctttgttacattactacattttgctttcatcagagagacattaaaggctgttaattcattgaataggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcaagttttctagtgtgttcaccattgcaggagaattttgctattgaacaaaagtgttaattggcaattccttgttaatatttcaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaagtatgtactccaactagtattcttattgaacttggtattttctggtcttggtgttttcggatgtccagctgacacattttcttccactgaagcagaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaaggtaggtcacttaatctagttcattcttgttatacaataggcaaaaagaattgttcaagtaacttgacttaacattgttctgcatgccttgaaattatcaaagaatagccaaagggaaagataccgtcttttcccttttggcatggagttatttcatattatataatatttttttatactctatttttacacaagatagattctttagaatattttattgatgtaataggcatggattaacactttttttaagatttccctgctagtgtgagtttgactagtgatgtcaacttctcctttggacttgaaattgtgtgctatatttgtgagattgaccattgaattttcgttgcagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaagtaagtttatcctcatttgcctgctctttaaagtttttgcttttgtatatcaaaatgatgatgcaaaaaaaaagtgacaactagctagggattttttccaaataggaatatcattttttctaataccagtatattttagccattgcgtccaacttcaagtttattatatttcctatcaatctagtcatttgatacctgcttgatttattcttacattttctactgtaaataaagatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggttagttgattcctgattttgaatatgcttgctctatatatatctctaatgtgacatttttcaatttactctgaattcgcttattgatgtttttgtaggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagaggtatacattcagatttacattatgagagcgtgttttcttgatttctctgttcacaatcaagaagttatttgacgattaagtgtcttcactgcaaattgcagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggtccgtactgattctctatagttaggtgattttttgttatttatgaattgctgttgtattaacttttttggttgcatctgcgtcccttgtgcttccaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggtatgcgccatttctgtctacaacaatggcacatgaagatcactcgctattttctagatctcttacagtcccctcatccgtttgtaatcaaacgacttttttttttcttcaggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgaggcatgttcatcttgacaggaaaaaaaaaatctatccatcactactgaaatctttgaaaatgtcctgaaaaactcatcaacatcttgttgcagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgataggctggtgcaatgcaatgcgatggcgccaaggatctgatactagttttcaagcctgtatacagaaatgtttttacagatacaagtcttctaaagctgtttcttttcattgtcgccttgtattttttattcttctgagtgtaaatttctcgagaaggtgtacaaatttctgttactgttgtagagacccagaataaataaaagtataggcattggtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051627.1 RefSeq:Os01g0898300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Fig.3.jpg&diff=176655 2014-06-03T10:39:04Z

<p>Wuxt: The Oryza sativa short postembryonic roots 1 (OsSPR1) expression pattern revealed by GUS staining and RT-PCR. The images show the GUS staining pattern obtained in OsSPR1 promoter:GUS transgenic plants in (a) a primary root; (b) a primary root elongation z</p> <hr /> <div>The Oryza sativa short postembryonic roots 1 (OsSPR1) expression pattern revealed by GUS staining and RT-PCR. The images show the GUS staining pattern obtained in OsSPR1 promoter:GUS transgenic plants in (a) a primary root; (b) a primary root elongation zone; (c) a primary root tip; (d) a mature lateral root; (e) the base of a mature lateral root; (f) a mature lateral root tip; (g) an elongating lateral root; (h) an elongating lateral root tip; (i) the node region of a young uppermost internode; (j) an auricle; (k) a cross-section of an uppermost node; (l) a cross-section of a stem; (m) young spikelets; (n) flowering spikelets; (o) the pedicel of a young panicle; (p) pistils; (q) a stem; (r) a leaf. Bars: (a, d, g, k–p) 100 lm; (i, j, q, r) 200 lm; (b, c, e, f, h) 50 lm. (s) Expression pattern of OsSPR1 obtained using RT-PCR. cDNA was amplified from the root (R), stem base (SB), stem (S), leaf (L), and panicles (P) for the wild type (WT) and spr1 mutant (mutant), respectively.</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Fig.6e.jpg&diff=176652 2014-06-03T10:36:20Z

<p>Wuxt: The Fe contents of roots of 15-d-old seedlings growing under different Fe supplies.</p> <hr /> <div>The Fe contents of roots of 15-d-old seedlings growing under different Fe supplies.</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Fig.6c.jpg&diff=176651 2014-06-03T10:34:59Z

<p>Wuxt: Chlorophyll content in the leaves of 15-d-old wild-type and spr1 seedlings grown in Fe(II) and Fe(III) solutions. The chlorophyll content of the fully expanded youngest leaf was measured using a SPAD-502 chlorophyll meter.</p> <hr /> <div>Chlorophyll content in the leaves of 15-d-old wild-type and spr1 seedlings grown in Fe(II) and Fe(III) solutions. The chlorophyll content of the fully expanded youngest leaf was measured using a SPAD-502 chlorophyll meter.</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=File:Fig.7.jpg&diff=176650 2014-06-03T10:33:49Z

<p>Wuxt: Semiquantitative RT-PCR analysis of the iron (Fe)-responsive genes. RNA was isolated from roots and leaves of 2-wk-old seedlings grown in culture solution with Fe(III) or Fe(II) supply, or without Fe ()Fe). OsActin primers were used as an endogenous contr</p> <hr /> <div>Semiquantitative RT-PCR analysis of the iron (Fe)-responsive genes. RNA was isolated from roots and leaves of 2-wk-old seedlings grown in culture solution with Fe(III) or Fe(II) supply, or without Fe ()Fe). OsActin primers were used as an endogenous control. WL, wild-type leaf; WR, wild-type root; SL, spr1 leaf; SR, spr1 root; NAS, nicotianamine synthase; NRAMP, natural resistanceassociated macrophage protein; YSL, yellow stripe-like.</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0898300&diff=176648 2014-06-03T10:29:01Z

<p>Wuxt: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> OsSPR1 was identified by map-based cloning. It encodes a novel mitochondrial protein with the Armadillo-like repeat domain. Osspr1 mutants exhibited decreased root cell elongation. The large reduction of Fe content observed in the leaves but not the roots of Osspr1 mutant plants under Fe(II) and Fe(III) nutrient supply, and the normal Fe deficiency response for both strategy I and II marker genes in the leaves but not the roots indicate a defect in Fe homeostasis. In addition, root development was also compromised in Osspr1 mutant plants. It is likely that the defective roots of Osspr1 plants will have a secondary effect on nutrient uptake. However, if reduced Fe uptake as a result of damaged roots was the primary reason for the reduced Fe content in the leaves, the Fe content and the Fe deficiency response would have been duced and activated, respectively, in the roots. However, the Fe content in roots of Osspr1 mutant plants was similar to that of wild-type plants (Fig. 6e). Also, no Fe deficiency response was observed in roots (Fig. 7). However, a lower Fe content was observed in the mutant leaves, which was inconsistent with the induction of Fe-responsive genes (Figs 6c, 7). These results indicate that OsSPR1 plays a role in maintaining Fe (and other metal) homeostasis between roots and leaves.<br /> <br /> ===Expression===<br /> <br /> The expression pattern of OsSPR1 was determined usingt he GUS gene driven by the OsSPR1 promoter transferred into wild-type plants. GUS staining in transgenic plants indicated that OsSPR1 was ubiquitously expressed in all plant organs, including the root, leaf, stem and spikelet (Fig. 3a–r). OsSPR1 was expressed in primary and lateral roots, both in mature regions and in root tips. Strong expression was observed in the root tip (in the meristem and lateral root initiation regions), where dividing cells are present. The expression of OsSPR1 in the root is mainly associated with vascular tissues (Fig. 3b,d,h). Semiquantitative RT-PCR analysis using SPR1-specific primers showed that SPR1 was expressed at relatively similar levelsin all tissues, including the root, stem base, stem, leaf and panicles (Fig. 3s), consistent with the GUS staining patterns observed.<br /> <br /> ===Evolution===<br /> Root elongation is determined by two successive processes: cell elongation and cell proliferation (Beemster et al., 2003). In rice, several genes controlling division and differentiation in root growth and development have been identified through genetic screens (Rebouillat et al., 2009). Here, we cloned OsSPR1, a novel mitochondrial gene involved in root cell elongation. Loss of function of OsSPR1 resulted in defective root elongation, including primary root, adventitious root and lateral root elongation; notably, the defects were more severe in lateral roots and adventitious roots. Analysis of the different root zones revealed similar arrangements of cells in those zones compared with the wild type, but reduced cell length was observed in the spr1 roots,<br /> suggesting that reduced root length in spr1 resulted from reduced cell elongation.<br /> ==Labs working on this gene==<br /> *Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Ghent University, Belgium.<br /> *State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China <br /> *ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, 6009 WA, Australia<br /> *Joint Laboratory in Genomics and Nutriomics, College of Life Science, Zhejiang University, Hangzhou 310058, China<br /> *Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China<br /> <br /> ==References==<br /> 1.Beemster GTS, Fiorani F, Inze´ D. 2003. Cell cycle: the key to plant growth control? Trends in Plant Science 8: 154–158.<br /> <br /> 2.Rebouillat J, Dievart A, Verdeil J, Escoute J, Giese G, Breitler J, Gantet P, Espeout S, Guiderdoni E, Pe´rin C. 2009. Molecular genetics of rice root development. Rice 2: 15–34.<br /> <br /> 3.Liqiang Jia;Zhongchang Wu;Xi Hao;Chris Carrie;Libin Zheng;James Whelan;Yunrong Wu;Shoufeng Wang;Ping Wu;Chuanzao Mao. Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa.New Phytologist, 2011, 189(3): 843-855.<br /> <br /> 4.Jia LiQiang . Is Reactive Oxygen Species (ROS) the underlying factor for inhibited root growth in Osspr1? Plant Signaling &amp; Behavior, 2011, 6(7): 1024-1025.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0898300|<br /> Description = Armadillo-like helical domain containing protein|<br /> Version = NM_001051627.1 GI:115441624 GeneID:4325109|<br /> Length = 6243 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0898300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:40808134..40814376|<br /> CDS = 40808350..40808496,40808589..40808780,40808893..40809024,40809123..40809260,40809362..40809562&lt;br&gt;,40809661..40809788,40810023..40810098,40810453..40810602,40810698..40811057&lt;br&gt;,40811144..40811296,40811384..40811676,40811792..40811988,40812071..40812228&lt;br&gt;,40812533..40812580,40812912..40813040,40813123..40813237,40813321..40813436&lt;br&gt;,40813737..40813811,40813982..40814116|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaaaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaaatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgatag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MGVMSRRVLPACSSLCYFCPSLRARSRQPVKRYKKIIAEIYQLP PDGEPNDRRIGKLCDYVSRNPTRIPKITEYLEERCYKDLRHENFTLAKVVPCIYRKLL CSCKDHTPLLATSTLSIIRTLLDQRMNDDLRVLGCLMLVDFLNGQVDSTHMFNLEGLI PKLCQISQELREDDKGFRLRCAALQALASMVQYMGDHSHISMELDEVVSVIVSCYEVN QTLSIKEVVRLQDDDDLVINGSLTGLPVSGQNSAKVASDTMSASENPAHWARVCLRNM ASIAKEATTVWRVLDPLFRLFDSHNYWSPENGIAFSILQEMQALMDKSGQNGHLLLSF TIKHIDHKSVAKKPAKQTSILKVASLLAKHAKLKASVTIASATSDLIKHLRKCMHCAV ESPNAQNDVDKWNSALYVALEECLVQLTEKVGDVGPVLDMVGVMLENLSCTATIARTT ISSVFRTVQIAASIHKSLYNQKAFPEALFHQLLLAMMHPDKKTRVGSHRVLSTIIAPS LLCPWSGISFPIPVKGNDSQSITLLALSAFSSEAVMDEVRIKSRTHEQLQNNVKPETV VGSENGYTHTEPNSRKSPGLGIPLKDENLKFMKLNSSQLVLLLSSIWSQAPLEDNSPA NFEAMCHTYNIALLCSMTKSSSHAALVRCFQLAFSLRRMSLNQENGLQPSRRRCLYTM ASAMLIFSAKVADIPQTIPLVKAAVPEKMVDPHLCLIDDCRLVISSPQSSNSGIVYGS EEDESDARNFLSCVNKNDTQLKEIVISHFKEKFENLSEKFNGIEEQLLQEFSLDDSFP LSAPLFMETPHSCSMYAEKDDHCFDEEVIPCEMDDDDDIVFEHSGSQSDRKTSGSMAS SDVLNVNQLIESVHETARQVANAPVSANLVPYDQMKSQCEALVMEKQQKMSVLLSFKH SRTDSRGSTAENGLETNESSARSEPETQSTRKERMRRSDSASSESDRSFRLPPASPYD KFMRAAGR&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5881..6027#5597..5788#5353..5484#5117..5254#4815..5015#4589..4716#4279..4354#3775..3924#3320..3679#3081..3233#2701..2993#2389..2585#2149..2306#1797..1844#1337..1465#1140..1254#941..1056#566..640#261..395#gaggtttgatcttggtttcgatccgaagggtctcggttggctgcgtgggaagggggaggaggaagaaggcgacctgctatctaatcctctaccccctctgctgcgaaaattctctgctgtttgctcaagcatcttgctgcttggagccttggatcgaagagagttttagttcttggagttctgagaattgtgttgcttgagccgaattcgatcccaaaaaggctcttttttgggaggctttgaggaggaggaggaagaagatgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggtatggcgattactgaatcctgtcttggcatttcagccatttctgatgcttagttgtagtctctgcgatttgggtctgtggtcaaatagttctagtttctgcgaattggagtctgtggtcaaatgtgtggagggagtttgttgattgagggtgtgatgttgttgctgtaggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaaggtaacacattttgatctcctctctgtttttactagaattgtcgcgcaatctgttagcttgaatagtttagactgaatttccagtatttctgaaagcagtctttataggtaccgagtagttaccacataatggctgatttacactgcattgctttagtgtgatggtgattccttttgccagagtacaaccgatcaactttcgctagcaccgatcactagctggaatgattttaggctgtttaagtaggacttaccgagatgaatgaatttgtggaactcggaactgattttatgtctgcagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacgtaagtgtaaaggctctattctgccagtcaagcacccatagcaaatgtttgctgctgtactgaccatattattcttgattcagaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttattgacgactgtaacaaattgcacctacttggtttgctagaaatctgccatctgtttggtttaatttgagttcatgtaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtaagaaaagtttttacttgattctctgatgaaatggataaggtagtcttatatgttgcgagctagccatttagggctaatttacagtagatttgttggaccagaaataataatgtagaacaaaataaggaactacattcaactattatacaatttgttttgcaaatataacatttttttaatgttggttatattgatattgcttgagaattcccaacagataatcctatacttgcattactggttcttgccaactgatgccattaaatcagtctattgattaaatttaactttttaatcctaatatactcatttaccactttgtaatcaggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagtaagtccttatcactctgtgttgggctagaatgttaagagttaagacttaattctcattcatggttgatagatgcctcctggtgaatatttgttctcttgttggaattgcataagattcatctttggagcatagacaagttgatgccattaatactacagatagacttgttgaataagtggaagatatttgattagtctatgaatagaaaaaaaaagtagcatgatagtttcatttgaagtgcgccagaaacagtaattcagtaagggttctgattagtctctgaattcattcttgaacccaggttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtgagaatttctgctaatgtaacggcccatttccacactcgatttgtgcccttgtcatcttaatgacttagtgtgttgacaggtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcaggtgcaaaatttttcacccatgctatttgtttctctttttgtgccattttttttggttttgttagtgtttaacagcagaaagatttagtgctcttctaaatcttgtttcttaacagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttcattatgctgtattattcctttgttacattactacattttgctttcatcagagagacattaaaggctgttaattcattgaataggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcaagttttctagtgtgttcaccattgcaggagaattttgctattgaacaaaagtgttaattggcaattccttgttaatatttcaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaagtatgtactccaactagtattcttattgaacttggtattttctggtcttggtgttttcggatgtccagctgacacattttcttccactgaagcagaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaaggtaggtcacttaatctagttcattcttgttatacaataggcaaaaagaattgttcaagtaacttgacttaacattgttctgcatgccttgaaattatcaaagaatagccaaagggaaagataccgtcttttcccttttggcatggagttatttcatattatataatatttttttatactctatttttacacaagatagattctttagaatattttattgatgtaataggcatggattaacactttttttaagatttccctgctagtgtgagtttgactagtgatgtcaacttctcctttggacttgaaattgtgtgctatatttgtgagattgaccattgaattttcgttgcagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaagtaagtttatcctcatttgcctgctctttaaagtttttgcttttgtatatcaaaatgatgatgcaaaaaaaaagtgacaactagctagggattttttccaaataggaatatcattttttctaataccagtatattttagccattgcgtccaacttcaagtttattatatttcctatcaatctagtcatttgatacctgcttgatttattcttacattttctactgtaaataaagatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggttagttgattcctgattttgaatatgcttgctctatatatatctctaatgtgacatttttcaatttactctgaattcgcttattgatgtttttgtaggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagaggtatacattcagatttacattatgagagcgtgttttcttgatttctctgttcacaatcaagaagttatttgacgattaagtgtcttcactgcaaattgcagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggtccgtactgattctctatagttaggtgattttttgttatttatgaattgctgttgtattaacttttttggttgcatctgcgtcccttgtgcttccaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggtatgcgccatttctgtctacaacaatggcacatgaagatcactcgctattttctagatctcttacagtcccctcatccgtttgtaatcaaacgacttttttttttcttcaggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgaggcatgttcatcttgacaggaaaaaaaaaatctatccatcactactgaaatctttgaaaatgtcctgaaaaactcatcaacatcttgttgcagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgataggctggtgcaatgcaatgcgatggcgccaaggatctgatactagttttcaagcctgtatacagaaatgtttttacagatacaagtcttctaaagctgtttcttttcattgtcgccttgtattttttattcttctgagtgtaaatttctcgagaaggtgtacaaatttctgttactgttgtagagacccagaataaataaaagtataggcattggtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051627.1 RefSeq:Os01g0898300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0898300&diff=176641 2014-06-03T10:20:34Z

<p>Wuxt: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> OsSPR1 was identified by map-based cloning. It encodes a novel mitochondrial protein with the Armadillo-like repeat domain. Osspr1 mutants exhibited decreased root cell elongation. The large reduction of Fe content observed in the leaves but not the roots of Osspr1 mutant plants under Fe(II) and Fe(III) nutrient supply, and the normal Fe deficiency response for both strategy I and II marker genes in the leaves but not the roots indicate a defect in Fe homeostasis. In addition, root development was also compromised in Osspr1 mutant plants. It is likely that the defective roots of Osspr1 plants will have a secondary effect on nutrient uptake. However, if reduced Fe uptake as a result of damaged roots was the primary reason for the reduced Fe content in the leaves, the Fe content and the Fe deficiency response would have been duced and activated, respectively, in the roots. However, the Fe content in roots of Osspr1 mutant plants was similar to that of wild-type plants (Fig. 6e). Also, no Fe deficiency response was observed in roots (Fig. 7). However, a lower Fe content was observed in the mutant leaves, which was inconsistent with the induction of Fe-responsive genes (Figs 6c, 7). These results indicate that OsSPR1 plays a role in maintaining Fe (and other metal) homeostasis between roots and leaves.<br /> <br /> ===Expression===<br /> <br /> The expression pattern of OsSPR1 was determined usingt he GUS gene driven by the OsSPR1 promoter transferred into wild-type plants. GUS staining in transgenic plants indicated that OsSPR1 was ubiquitously expressed in all plant organs, including the root, leaf, stem and spikelet (Fig. 3a–r). OsSPR1 was expressed in primary and lateral roots, both in mature regions and in root tips. Strong expression was observed in the root tip (in the meristem and lateral root initiation regions), where dividing cells are present. The expression of OsSPR1 in the root is mainly associated with vascular tissues (Fig. 3b,d,h). Semiquantitative RT-PCR analysis using SPR1-specific primers showed that SPR1 was expressed at relatively similar levelsin all tissues, including the root, stem base, stem, leaf and panicles (Fig. S3), consistent with the GUS staining patterns observed.<br /> <br /> ===Evolution===<br /> Root elongation is determined by two successive processes: cell elongation and cell proliferation (Beemster et al., 2003). In rice, several genes controlling division and differentiation in root growth and development have been identified through genetic screens (Rebouillat et al., 2009). Here, we cloned OsSPR1, a novel mitochondrial gene involved in root cell elongation. Loss of function of OsSPR1 resulted in defective root elongation, including primary root, adventitious root and lateral root elongation; notably, the defects were more severe in lateral roots and adventitious roots. Analysis of the different root zones revealed similar arrangements of cells in those zones compared with the wild type, but reduced cell length was observed in the spr1 roots,<br /> suggesting that reduced root length in spr1 resulted from reduced cell elongation.<br /> ==Labs working on this gene==<br /> *Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Ghent University, Belgium.<br /> <br /> ==References==<br /> 1.Beemster GTS, Fiorani F, Inze´ D. 2003. Cell cycle: the key to plant growth control? Trends in Plant Science 8: 154–158.<br /> <br /> 2.Rebouillat J, Dievart A, Verdeil J, Escoute J, Giese G, Breitler J, Gantet P, Espeout S, Guiderdoni E, Pe´rin C. 2009. Molecular genetics of rice root development. Rice 2: 15–34.<br /> <br /> 3.Liqiang Jia;Zhongchang Wu;Xi Hao;Chris Carrie;Libin Zheng;James Whelan;Yunrong Wu;Shoufeng Wang;Ping Wu;Chuanzao Mao. Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa.New Phytologist, 2011, 189(3): 843-855.<br /> <br /> 4.Jia LiQiang . Is Reactive Oxygen Species (ROS) the underlying factor for inhibited root growth in Osspr1? Plant Signaling &amp; Behavior, 2011, 6(7): 1024-1025.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0898300|<br /> Description = Armadillo-like helical domain containing protein|<br /> Version = NM_001051627.1 GI:115441624 GeneID:4325109|<br /> Length = 6243 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0898300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:40808134..40814376|<br /> CDS = 40808350..40808496,40808589..40808780,40808893..40809024,40809123..40809260,40809362..40809562&lt;br&gt;,40809661..40809788,40810023..40810098,40810453..40810602,40810698..40811057&lt;br&gt;,40811144..40811296,40811384..40811676,40811792..40811988,40812071..40812228&lt;br&gt;,40812533..40812580,40812912..40813040,40813123..40813237,40813321..40813436&lt;br&gt;,40813737..40813811,40813982..40814116|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaaaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaaatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgatag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MGVMSRRVLPACSSLCYFCPSLRARSRQPVKRYKKIIAEIYQLP PDGEPNDRRIGKLCDYVSRNPTRIPKITEYLEERCYKDLRHENFTLAKVVPCIYRKLL CSCKDHTPLLATSTLSIIRTLLDQRMNDDLRVLGCLMLVDFLNGQVDSTHMFNLEGLI PKLCQISQELREDDKGFRLRCAALQALASMVQYMGDHSHISMELDEVVSVIVSCYEVN QTLSIKEVVRLQDDDDLVINGSLTGLPVSGQNSAKVASDTMSASENPAHWARVCLRNM ASIAKEATTVWRVLDPLFRLFDSHNYWSPENGIAFSILQEMQALMDKSGQNGHLLLSF TIKHIDHKSVAKKPAKQTSILKVASLLAKHAKLKASVTIASATSDLIKHLRKCMHCAV ESPNAQNDVDKWNSALYVALEECLVQLTEKVGDVGPVLDMVGVMLENLSCTATIARTT ISSVFRTVQIAASIHKSLYNQKAFPEALFHQLLLAMMHPDKKTRVGSHRVLSTIIAPS LLCPWSGISFPIPVKGNDSQSITLLALSAFSSEAVMDEVRIKSRTHEQLQNNVKPETV VGSENGYTHTEPNSRKSPGLGIPLKDENLKFMKLNSSQLVLLLSSIWSQAPLEDNSPA NFEAMCHTYNIALLCSMTKSSSHAALVRCFQLAFSLRRMSLNQENGLQPSRRRCLYTM ASAMLIFSAKVADIPQTIPLVKAAVPEKMVDPHLCLIDDCRLVISSPQSSNSGIVYGS EEDESDARNFLSCVNKNDTQLKEIVISHFKEKFENLSEKFNGIEEQLLQEFSLDDSFP LSAPLFMETPHSCSMYAEKDDHCFDEEVIPCEMDDDDDIVFEHSGSQSDRKTSGSMAS SDVLNVNQLIESVHETARQVANAPVSANLVPYDQMKSQCEALVMEKQQKMSVLLSFKH SRTDSRGSTAENGLETNESSARSEPETQSTRKERMRRSDSASSESDRSFRLPPASPYD KFMRAAGR&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5881..6027#5597..5788#5353..5484#5117..5254#4815..5015#4589..4716#4279..4354#3775..3924#3320..3679#3081..3233#2701..2993#2389..2585#2149..2306#1797..1844#1337..1465#1140..1254#941..1056#566..640#261..395#gaggtttgatcttggtttcgatccgaagggtctcggttggctgcgtgggaagggggaggaggaagaaggcgacctgctatctaatcctctaccccctctgctgcgaaaattctctgctgtttgctcaagcatcttgctgcttggagccttggatcgaagagagttttagttcttggagttctgagaattgtgttgcttgagccgaattcgatcccaaaaaggctcttttttgggaggctttgaggaggaggaggaagaagatgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggtatggcgattactgaatcctgtcttggcatttcagccatttctgatgcttagttgtagtctctgcgatttgggtctgtggtcaaatagttctagtttctgcgaattggagtctgtggtcaaatgtgtggagggagtttgttgattgagggtgtgatgttgttgctgtaggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaaggtaacacattttgatctcctctctgtttttactagaattgtcgcgcaatctgttagcttgaatagtttagactgaatttccagtatttctgaaagcagtctttataggtaccgagtagttaccacataatggctgatttacactgcattgctttagtgtgatggtgattccttttgccagagtacaaccgatcaactttcgctagcaccgatcactagctggaatgattttaggctgtttaagtaggacttaccgagatgaatgaatttgtggaactcggaactgattttatgtctgcagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacgtaagtgtaaaggctctattctgccagtcaagcacccatagcaaatgtttgctgctgtactgaccatattattcttgattcagaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttattgacgactgtaacaaattgcacctacttggtttgctagaaatctgccatctgtttggtttaatttgagttcatgtaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtaagaaaagtttttacttgattctctgatgaaatggataaggtagtcttatatgttgcgagctagccatttagggctaatttacagtagatttgttggaccagaaataataatgtagaacaaaataaggaactacattcaactattatacaatttgttttgcaaatataacatttttttaatgttggttatattgatattgcttgagaattcccaacagataatcctatacttgcattactggttcttgccaactgatgccattaaatcagtctattgattaaatttaactttttaatcctaatatactcatttaccactttgtaatcaggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagtaagtccttatcactctgtgttgggctagaatgttaagagttaagacttaattctcattcatggttgatagatgcctcctggtgaatatttgttctcttgttggaattgcataagattcatctttggagcatagacaagttgatgccattaatactacagatagacttgttgaataagtggaagatatttgattagtctatgaatagaaaaaaaaagtagcatgatagtttcatttgaagtgcgccagaaacagtaattcagtaagggttctgattagtctctgaattcattcttgaacccaggttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtgagaatttctgctaatgtaacggcccatttccacactcgatttgtgcccttgtcatcttaatgacttagtgtgttgacaggtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcaggtgcaaaatttttcacccatgctatttgtttctctttttgtgccattttttttggttttgttagtgtttaacagcagaaagatttagtgctcttctaaatcttgtttcttaacagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttcattatgctgtattattcctttgttacattactacattttgctttcatcagagagacattaaaggctgttaattcattgaataggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcaagttttctagtgtgttcaccattgcaggagaattttgctattgaacaaaagtgttaattggcaattccttgttaatatttcaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaagtatgtactccaactagtattcttattgaacttggtattttctggtcttggtgttttcggatgtccagctgacacattttcttccactgaagcagaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaaggtaggtcacttaatctagttcattcttgttatacaataggcaaaaagaattgttcaagtaacttgacttaacattgttctgcatgccttgaaattatcaaagaatagccaaagggaaagataccgtcttttcccttttggcatggagttatttcatattatataatatttttttatactctatttttacacaagatagattctttagaatattttattgatgtaataggcatggattaacactttttttaagatttccctgctagtgtgagtttgactagtgatgtcaacttctcctttggacttgaaattgtgtgctatatttgtgagattgaccattgaattttcgttgcagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaagtaagtttatcctcatttgcctgctctttaaagtttttgcttttgtatatcaaaatgatgatgcaaaaaaaaagtgacaactagctagggattttttccaaataggaatatcattttttctaataccagtatattttagccattgcgtccaacttcaagtttattatatttcctatcaatctagtcatttgatacctgcttgatttattcttacattttctactgtaaataaagatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggttagttgattcctgattttgaatatgcttgctctatatatatctctaatgtgacatttttcaatttactctgaattcgcttattgatgtttttgtaggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagaggtatacattcagatttacattatgagagcgtgttttcttgatttctctgttcacaatcaagaagttatttgacgattaagtgtcttcactgcaaattgcagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggtccgtactgattctctatagttaggtgattttttgttatttatgaattgctgttgtattaacttttttggttgcatctgcgtcccttgtgcttccaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggtatgcgccatttctgtctacaacaatggcacatgaagatcactcgctattttctagatctcttacagtcccctcatccgtttgtaatcaaacgacttttttttttcttcaggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgaggcatgttcatcttgacaggaaaaaaaaaatctatccatcactactgaaatctttgaaaatgtcctgaaaaactcatcaacatcttgttgcagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgataggctggtgcaatgcaatgcgatggcgccaaggatctgatactagttttcaagcctgtatacagaaatgtttttacagatacaagtcttctaaagctgtttcttttcattgtcgccttgtattttttattcttctgagtgtaaatttctcgagaaggtgtacaaatttctgttactgttgtagagacccagaataaataaaagtataggcattggtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051627.1 RefSeq:Os01g0898300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0898300&diff=176633 2014-06-03T10:13:34Z

<p>Wuxt: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> This study has identified a novel mitochondrial protein, OsSPR1, that is involved in maintaining Fe homeostasis in rice. The large reduction of Fe content observed in the leaves but not the roots of Osspr1 mutant plants under Fe(II) and Fe(III) nutrient supply, and the normal Fe deficiency response for both strategy I and II marker genes in the leaves but not the roots indicate a defect in Fe homeostasis. In addition, root development was also compromised in Osspr1 mutant plants. It is likely that the defective roots of Osspr1 plants will have a secondary effect on nutrient uptake. However, if reduced Fe uptake as a result of damaged roots was the primary reason for the reduced Fe content in the leaves, the Fe content and the Fe deficiency response would have been duced and activated, respectively, in the roots. However, the Fe content in roots of Osspr1 mutant plants was similar to that of wild-type plants (Fig. 6e). Also, no Fe deficiency response was observed in roots (Fig. 7). However, a lower Fe content was observed in the mutant leaves, which was inconsistent with the induction of Fe-responsive genes (Figs 6c, 7). These results indicate that OsSPR1 plays a role in maintaining Fe (and other metal) homeostasis between roots and leaves.<br /> <br /> ===Expression===<br /> <br /> The expression pattern of OsSPR1 was determined usingt he GUS gene driven by the OsSPR1 promoter transferred into wild-type plants. GUS staining in transgenic plants indicated that OsSPR1 was ubiquitously expressed in all plant organs, including the root, leaf, stem and spikelet (Fig. 3a–r). OsSPR1 was expressed in primary and lateral roots, both in mature regions and in root tips. Strong expression was observed in the root tip (in the meristem and lateral root initiation regions), where dividing cells are present. The expression of OsSPR1 in the root is mainly associated with vascular tissues (Fig. 3b,d,h). Semiquantitative RT-PCR analysis using SPR1-specific primers showed that SPR1 was expressed at relatively similar levelsin all tissues, including the root, stem base, stem, leaf and panicles (Fig. S3), consistent with the GUS staining patterns observed.<br /> <br /> ===Evolution===<br /> Root elongation is determined by two successive processes: cell elongation and cell proliferation (Beemster et al., 2003). In rice, several genes controlling division and differentiation in root growth and development have been identified through genetic screens (Rebouillat et al., 2009). Here, we cloned OsSPR1, a novel mitochondrial gene involved in root cell elongation. Loss of function of OsSPR1 resulted in defective root elongation, including primary root, adventitious root and lateral root elongation; notably, the defects were more severe in lateral roots and adventitious roots. Analysis of the different root zones revealed similar arrangements of cells in those zones compared with the wild type, but reduced cell length was observed in the spr1 roots,<br /> suggesting that reduced root length in spr1 resulted from reduced cell elongation.<br /> ==Labs working on this gene==<br /> <br /> ==References==<br /> 1.Beemster GTS, Fiorani F, Inze´ D. 2003. Cell cycle: the key to plant growth control? Trends in Plant Science 8: 154–158.<br /> <br /> 2.Rebouillat J, Dievart A, Verdeil J, Escoute J, Giese G, Breitler J, Gantet P, Espeout S, Guiderdoni E, Pe´rin C. 2009. Molecular genetics of rice root development. Rice 2: 15–34.<br /> <br /> 3.Liqiang Jia;Zhongchang Wu;Xi Hao;Chris Carrie;Libin Zheng;James Whelan;Yunrong Wu;Shoufeng Wang;Ping Wu;Chuanzao Mao. Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa New Phytologist, 2011, 189(3): 843-855.<br /> <br /> 4.Jia LiQiang . Is Reactive Oxygen Species (ROS) the underlying factor for inhibited root growth in Osspr1? Plant Signaling &amp; Behavior, 2011, 6(7): 1024-1025.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0898300|<br /> Description = Armadillo-like helical domain containing protein|<br /> Version = NM_001051627.1 GI:115441624 GeneID:4325109|<br /> Length = 6243 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0898300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:40808134..40814376|<br /> CDS = 40808350..40808496,40808589..40808780,40808893..40809024,40809123..40809260,40809362..40809562&lt;br&gt;,40809661..40809788,40810023..40810098,40810453..40810602,40810698..40811057&lt;br&gt;,40811144..40811296,40811384..40811676,40811792..40811988,40812071..40812228&lt;br&gt;,40812533..40812580,40812912..40813040,40813123..40813237,40813321..40813436&lt;br&gt;,40813737..40813811,40813982..40814116|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaaaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaaatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgatag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MGVMSRRVLPACSSLCYFCPSLRARSRQPVKRYKKIIAEIYQLP PDGEPNDRRIGKLCDYVSRNPTRIPKITEYLEERCYKDLRHENFTLAKVVPCIYRKLL CSCKDHTPLLATSTLSIIRTLLDQRMNDDLRVLGCLMLVDFLNGQVDSTHMFNLEGLI PKLCQISQELREDDKGFRLRCAALQALASMVQYMGDHSHISMELDEVVSVIVSCYEVN QTLSIKEVVRLQDDDDLVINGSLTGLPVSGQNSAKVASDTMSASENPAHWARVCLRNM ASIAKEATTVWRVLDPLFRLFDSHNYWSPENGIAFSILQEMQALMDKSGQNGHLLLSF TIKHIDHKSVAKKPAKQTSILKVASLLAKHAKLKASVTIASATSDLIKHLRKCMHCAV ESPNAQNDVDKWNSALYVALEECLVQLTEKVGDVGPVLDMVGVMLENLSCTATIARTT ISSVFRTVQIAASIHKSLYNQKAFPEALFHQLLLAMMHPDKKTRVGSHRVLSTIIAPS LLCPWSGISFPIPVKGNDSQSITLLALSAFSSEAVMDEVRIKSRTHEQLQNNVKPETV VGSENGYTHTEPNSRKSPGLGIPLKDENLKFMKLNSSQLVLLLSSIWSQAPLEDNSPA NFEAMCHTYNIALLCSMTKSSSHAALVRCFQLAFSLRRMSLNQENGLQPSRRRCLYTM ASAMLIFSAKVADIPQTIPLVKAAVPEKMVDPHLCLIDDCRLVISSPQSSNSGIVYGS EEDESDARNFLSCVNKNDTQLKEIVISHFKEKFENLSEKFNGIEEQLLQEFSLDDSFP LSAPLFMETPHSCSMYAEKDDHCFDEEVIPCEMDDDDDIVFEHSGSQSDRKTSGSMAS SDVLNVNQLIESVHETARQVANAPVSANLVPYDQMKSQCEALVMEKQQKMSVLLSFKH SRTDSRGSTAENGLETNESSARSEPETQSTRKERMRRSDSASSESDRSFRLPPASPYD KFMRAAGR&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5881..6027#5597..5788#5353..5484#5117..5254#4815..5015#4589..4716#4279..4354#3775..3924#3320..3679#3081..3233#2701..2993#2389..2585#2149..2306#1797..1844#1337..1465#1140..1254#941..1056#566..640#261..395#gaggtttgatcttggtttcgatccgaagggtctcggttggctgcgtgggaagggggaggaggaagaaggcgacctgctatctaatcctctaccccctctgctgcgaaaattctctgctgtttgctcaagcatcttgctgcttggagccttggatcgaagagagttttagttcttggagttctgagaattgtgttgcttgagccgaattcgatcccaaaaaggctcttttttgggaggctttgaggaggaggaggaagaagatgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggtatggcgattactgaatcctgtcttggcatttcagccatttctgatgcttagttgtagtctctgcgatttgggtctgtggtcaaatagttctagtttctgcgaattggagtctgtggtcaaatgtgtggagggagtttgttgattgagggtgtgatgttgttgctgtaggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaaggtaacacattttgatctcctctctgtttttactagaattgtcgcgcaatctgttagcttgaatagtttagactgaatttccagtatttctgaaagcagtctttataggtaccgagtagttaccacataatggctgatttacactgcattgctttagtgtgatggtgattccttttgccagagtacaaccgatcaactttcgctagcaccgatcactagctggaatgattttaggctgtttaagtaggacttaccgagatgaatgaatttgtggaactcggaactgattttatgtctgcagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacgtaagtgtaaaggctctattctgccagtcaagcacccatagcaaatgtttgctgctgtactgaccatattattcttgattcagaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttattgacgactgtaacaaattgcacctacttggtttgctagaaatctgccatctgtttggtttaatttgagttcatgtaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtaagaaaagtttttacttgattctctgatgaaatggataaggtagtcttatatgttgcgagctagccatttagggctaatttacagtagatttgttggaccagaaataataatgtagaacaaaataaggaactacattcaactattatacaatttgttttgcaaatataacatttttttaatgttggttatattgatattgcttgagaattcccaacagataatcctatacttgcattactggttcttgccaactgatgccattaaatcagtctattgattaaatttaactttttaatcctaatatactcatttaccactttgtaatcaggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagtaagtccttatcactctgtgttgggctagaatgttaagagttaagacttaattctcattcatggttgatagatgcctcctggtgaatatttgttctcttgttggaattgcataagattcatctttggagcatagacaagttgatgccattaatactacagatagacttgttgaataagtggaagatatttgattagtctatgaatagaaaaaaaaagtagcatgatagtttcatttgaagtgcgccagaaacagtaattcagtaagggttctgattagtctctgaattcattcttgaacccaggttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtgagaatttctgctaatgtaacggcccatttccacactcgatttgtgcccttgtcatcttaatgacttagtgtgttgacaggtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcaggtgcaaaatttttcacccatgctatttgtttctctttttgtgccattttttttggttttgttagtgtttaacagcagaaagatttagtgctcttctaaatcttgtttcttaacagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttcattatgctgtattattcctttgttacattactacattttgctttcatcagagagacattaaaggctgttaattcattgaataggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcaagttttctagtgtgttcaccattgcaggagaattttgctattgaacaaaagtgttaattggcaattccttgttaatatttcaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaagtatgtactccaactagtattcttattgaacttggtattttctggtcttggtgttttcggatgtccagctgacacattttcttccactgaagcagaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaaggtaggtcacttaatctagttcattcttgttatacaataggcaaaaagaattgttcaagtaacttgacttaacattgttctgcatgccttgaaattatcaaagaatagccaaagggaaagataccgtcttttcccttttggcatggagttatttcatattatataatatttttttatactctatttttacacaagatagattctttagaatattttattgatgtaataggcatggattaacactttttttaagatttccctgctagtgtgagtttgactagtgatgtcaacttctcctttggacttgaaattgtgtgctatatttgtgagattgaccattgaattttcgttgcagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaagtaagtttatcctcatttgcctgctctttaaagtttttgcttttgtatatcaaaatgatgatgcaaaaaaaaagtgacaactagctagggattttttccaaataggaatatcattttttctaataccagtatattttagccattgcgtccaacttcaagtttattatatttcctatcaatctagtcatttgatacctgcttgatttattcttacattttctactgtaaataaagatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggttagttgattcctgattttgaatatgcttgctctatatatatctctaatgtgacatttttcaatttactctgaattcgcttattgatgtttttgtaggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagaggtatacattcagatttacattatgagagcgtgttttcttgatttctctgttcacaatcaagaagttatttgacgattaagtgtcttcactgcaaattgcagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggtccgtactgattctctatagttaggtgattttttgttatttatgaattgctgttgtattaacttttttggttgcatctgcgtcccttgtgcttccaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggtatgcgccatttctgtctacaacaatggcacatgaagatcactcgctattttctagatctcttacagtcccctcatccgtttgtaatcaaacgacttttttttttcttcaggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgaggcatgttcatcttgacaggaaaaaaaaaatctatccatcactactgaaatctttgaaaatgtcctgaaaaactcatcaacatcttgttgcagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgataggctggtgcaatgcaatgcgatggcgccaaggatctgatactagttttcaagcctgtatacagaaatgtttttacagatacaagtcttctaaagctgtttcttttcattgtcgccttgtattttttattcttctgagtgtaaatttctcgagaaggtgtacaaatttctgttactgttgtagagacccagaataaataaaagtataggcattggtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051627.1 RefSeq:Os01g0898300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0898300&diff=176632 2014-06-03T10:13:21Z

<p>Wuxt: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> <br /> This study has identified a novel mitochondrial protein, OsSPR1, that is involved in maintaining Fe homeostasis in rice. The large reduction of Fe content observed in the leaves but not the roots of Osspr1 mutant plants under Fe(II) and Fe(III) nutrient supply, and the normal Fe deficiency response for both strategy I and II marker genes in the leaves but not the roots indicate a defect in Fe homeostasis. In addition, root development was also compromised in Osspr1 mutant plants. It is likely that the defective roots of Osspr1 plants will have a secondary effect on nutrient uptake. However, if reduced Fe uptake as a result of damaged roots was the primary reason for the reduced Fe content in the leaves, the Fe content and the Fe deficiency response would have been duced and activated, respectively, in the roots. However, the Fe content in roots of Osspr1 mutant plants was similar to that of wild-type plants (Fig. 6e). Also, no Fe deficiency response was observed in roots (Fig. 7). However, a lower Fe content was observed in the mutant leaves, which was inconsistent with the induction of Fe-responsive genes (Figs 6c, 7). These results indicate that OsSPR1 plays a role in maintaining Fe (and other metal) homeostasis between roots and leaves.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The expression pattern of OsSPR1 was determined usingt he GUS gene driven by the OsSPR1 promoter transferred into wild-type plants. GUS staining in transgenic plants indicated that OsSPR1 was ubiquitously expressed in all plant organs, including the root, leaf, stem and spikelet (Fig. 3a–r). OsSPR1 was expressed in primary and lateral roots, both in mature regions and in root tips. Strong expression was observed in the root tip (in the meristem and lateral root initiation regions), where dividing cells are present. The expression of OsSPR1 in the root is mainly associated with vascular tissues (Fig. 3b,d,h). Semiquantitative RT-PCR analysis using SPR1-specific primers showed that SPR1 was expressed at relatively similar levelsin all tissues, including the root, stem base, stem, leaf and panicles (Fig. S3), consistent with the GUS staining patterns observed.<br /> <br /> ===Evolution===<br /> Root elongation is determined by two successive processes: cell elongation and cell proliferation (Beemster et al., 2003). In rice, several genes controlling division and differentiation in root growth and development have been identified through genetic screens (Rebouillat et al., 2009). Here, we cloned OsSPR1, a novel mitochondrial gene involved in root cell elongation. Loss of function of OsSPR1 resulted in defective root elongation, including primary root, adventitious root and lateral root elongation; notably, the defects were more severe in lateral roots and adventitious roots. Analysis of the different root zones revealed similar arrangements of cells in those zones compared with the wild type, but reduced cell length was observed in the spr1 roots,<br /> suggesting that reduced root length in spr1 resulted from reduced cell elongation.<br /> ==Labs working on this gene==<br /> <br /> ==References==<br /> 1.Beemster GTS, Fiorani F, Inze´ D. 2003. Cell cycle: the key to plant growth control? Trends in Plant Science 8: 154–158.<br /> <br /> 2.Rebouillat J, Dievart A, Verdeil J, Escoute J, Giese G, Breitler J, Gantet P, Espeout S, Guiderdoni E, Pe´rin C. 2009. Molecular genetics of rice root development. Rice 2: 15–34.<br /> <br /> 3.Liqiang Jia;Zhongchang Wu;Xi Hao;Chris Carrie;Libin Zheng;James Whelan;Yunrong Wu;Shoufeng Wang;Ping Wu;Chuanzao Mao. Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa New Phytologist, 2011, 189(3): 843-855.<br /> <br /> 4.Jia LiQiang . Is Reactive Oxygen Species (ROS) the underlying factor for inhibited root growth in Osspr1? Plant Signaling &amp; Behavior, 2011, 6(7): 1024-1025.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0898300|<br /> Description = Armadillo-like helical domain containing protein|<br /> Version = NM_001051627.1 GI:115441624 GeneID:4325109|<br /> Length = 6243 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0898300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:40808134..40814376|<br /> CDS = 40808350..40808496,40808589..40808780,40808893..40809024,40809123..40809260,40809362..40809562&lt;br&gt;,40809661..40809788,40810023..40810098,40810453..40810602,40810698..40811057&lt;br&gt;,40811144..40811296,40811384..40811676,40811792..40811988,40812071..40812228&lt;br&gt;,40812533..40812580,40812912..40813040,40813123..40813237,40813321..40813436&lt;br&gt;,40813737..40813811,40813982..40814116|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaaaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaaatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgatag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MGVMSRRVLPACSSLCYFCPSLRARSRQPVKRYKKIIAEIYQLP PDGEPNDRRIGKLCDYVSRNPTRIPKITEYLEERCYKDLRHENFTLAKVVPCIYRKLL CSCKDHTPLLATSTLSIIRTLLDQRMNDDLRVLGCLMLVDFLNGQVDSTHMFNLEGLI PKLCQISQELREDDKGFRLRCAALQALASMVQYMGDHSHISMELDEVVSVIVSCYEVN QTLSIKEVVRLQDDDDLVINGSLTGLPVSGQNSAKVASDTMSASENPAHWARVCLRNM ASIAKEATTVWRVLDPLFRLFDSHNYWSPENGIAFSILQEMQALMDKSGQNGHLLLSF TIKHIDHKSVAKKPAKQTSILKVASLLAKHAKLKASVTIASATSDLIKHLRKCMHCAV ESPNAQNDVDKWNSALYVALEECLVQLTEKVGDVGPVLDMVGVMLENLSCTATIARTT ISSVFRTVQIAASIHKSLYNQKAFPEALFHQLLLAMMHPDKKTRVGSHRVLSTIIAPS LLCPWSGISFPIPVKGNDSQSITLLALSAFSSEAVMDEVRIKSRTHEQLQNNVKPETV VGSENGYTHTEPNSRKSPGLGIPLKDENLKFMKLNSSQLVLLLSSIWSQAPLEDNSPA NFEAMCHTYNIALLCSMTKSSSHAALVRCFQLAFSLRRMSLNQENGLQPSRRRCLYTM ASAMLIFSAKVADIPQTIPLVKAAVPEKMVDPHLCLIDDCRLVISSPQSSNSGIVYGS EEDESDARNFLSCVNKNDTQLKEIVISHFKEKFENLSEKFNGIEEQLLQEFSLDDSFP LSAPLFMETPHSCSMYAEKDDHCFDEEVIPCEMDDDDDIVFEHSGSQSDRKTSGSMAS SDVLNVNQLIESVHETARQVANAPVSANLVPYDQMKSQCEALVMEKQQKMSVLLSFKH SRTDSRGSTAENGLETNESSARSEPETQSTRKERMRRSDSASSESDRSFRLPPASPYD KFMRAAGR&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5881..6027#5597..5788#5353..5484#5117..5254#4815..5015#4589..4716#4279..4354#3775..3924#3320..3679#3081..3233#2701..2993#2389..2585#2149..2306#1797..1844#1337..1465#1140..1254#941..1056#566..640#261..395#gaggtttgatcttggtttcgatccgaagggtctcggttggctgcgtgggaagggggaggaggaagaaggcgacctgctatctaatcctctaccccctctgctgcgaaaattctctgctgtttgctcaagcatcttgctgcttggagccttggatcgaagagagttttagttcttggagttctgagaattgtgttgcttgagccgaattcgatcccaaaaaggctcttttttgggaggctttgaggaggaggaggaagaagatgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggtatggcgattactgaatcctgtcttggcatttcagccatttctgatgcttagttgtagtctctgcgatttgggtctgtggtcaaatagttctagtttctgcgaattggagtctgtggtcaaatgtgtggagggagtttgttgattgagggtgtgatgttgttgctgtaggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaaggtaacacattttgatctcctctctgtttttactagaattgtcgcgcaatctgttagcttgaatagtttagactgaatttccagtatttctgaaagcagtctttataggtaccgagtagttaccacataatggctgatttacactgcattgctttagtgtgatggtgattccttttgccagagtacaaccgatcaactttcgctagcaccgatcactagctggaatgattttaggctgtttaagtaggacttaccgagatgaatgaatttgtggaactcggaactgattttatgtctgcagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacgtaagtgtaaaggctctattctgccagtcaagcacccatagcaaatgtttgctgctgtactgaccatattattcttgattcagaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttattgacgactgtaacaaattgcacctacttggtttgctagaaatctgccatctgtttggtttaatttgagttcatgtaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtaagaaaagtttttacttgattctctgatgaaatggataaggtagtcttatatgttgcgagctagccatttagggctaatttacagtagatttgttggaccagaaataataatgtagaacaaaataaggaactacattcaactattatacaatttgttttgcaaatataacatttttttaatgttggttatattgatattgcttgagaattcccaacagataatcctatacttgcattactggttcttgccaactgatgccattaaatcagtctattgattaaatttaactttttaatcctaatatactcatttaccactttgtaatcaggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagtaagtccttatcactctgtgttgggctagaatgttaagagttaagacttaattctcattcatggttgatagatgcctcctggtgaatatttgttctcttgttggaattgcataagattcatctttggagcatagacaagttgatgccattaatactacagatagacttgttgaataagtggaagatatttgattagtctatgaatagaaaaaaaaagtagcatgatagtttcatttgaagtgcgccagaaacagtaattcagtaagggttctgattagtctctgaattcattcttgaacccaggttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtgagaatttctgctaatgtaacggcccatttccacactcgatttgtgcccttgtcatcttaatgacttagtgtgttgacaggtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcaggtgcaaaatttttcacccatgctatttgtttctctttttgtgccattttttttggttttgttagtgtttaacagcagaaagatttagtgctcttctaaatcttgtttcttaacagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttcattatgctgtattattcctttgttacattactacattttgctttcatcagagagacattaaaggctgttaattcattgaataggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcaagttttctagtgtgttcaccattgcaggagaattttgctattgaacaaaagtgttaattggcaattccttgttaatatttcaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaagtatgtactccaactagtattcttattgaacttggtattttctggtcttggtgttttcggatgtccagctgacacattttcttccactgaagcagaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaaggtaggtcacttaatctagttcattcttgttatacaataggcaaaaagaattgttcaagtaacttgacttaacattgttctgcatgccttgaaattatcaaagaatagccaaagggaaagataccgtcttttcccttttggcatggagttatttcatattatataatatttttttatactctatttttacacaagatagattctttagaatattttattgatgtaataggcatggattaacactttttttaagatttccctgctagtgtgagtttgactagtgatgtcaacttctcctttggacttgaaattgtgtgctatatttgtgagattgaccattgaattttcgttgcagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaagtaagtttatcctcatttgcctgctctttaaagtttttgcttttgtatatcaaaatgatgatgcaaaaaaaaagtgacaactagctagggattttttccaaataggaatatcattttttctaataccagtatattttagccattgcgtccaacttcaagtttattatatttcctatcaatctagtcatttgatacctgcttgatttattcttacattttctactgtaaataaagatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggttagttgattcctgattttgaatatgcttgctctatatatatctctaatgtgacatttttcaatttactctgaattcgcttattgatgtttttgtaggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagaggtatacattcagatttacattatgagagcgtgttttcttgatttctctgttcacaatcaagaagttatttgacgattaagtgtcttcactgcaaattgcagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggtccgtactgattctctatagttaggtgattttttgttatttatgaattgctgttgtattaacttttttggttgcatctgcgtcccttgtgcttccaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggtatgcgccatttctgtctacaacaatggcacatgaagatcactcgctattttctagatctcttacagtcccctcatccgtttgtaatcaaacgacttttttttttcttcaggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgaggcatgttcatcttgacaggaaaaaaaaaatctatccatcactactgaaatctttgaaaatgtcctgaaaaactcatcaacatcttgttgcagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgataggctggtgcaatgcaatgcgatggcgccaaggatctgatactagttttcaagcctgtatacagaaatgtttttacagatacaagtcttctaaagctgtttcttttcattgtcgccttgtattttttattcttctgagtgtaaatttctcgagaaggtgtacaaatttctgttactgttgtagagacccagaataaataaaagtataggcattggtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051627.1 RefSeq:Os01g0898300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0898300&diff=176627 2014-06-03T10:10:47Z

<p>Wuxt: Undo revision 176623 by Wuxt (talk)</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study has identified a novel mitochondrial protein, OsSPR1, that is involved in maintaining Fe homeostasis in rice. The large reduction of Fe content observed in the leaves but not the roots of Osspr1 mutant plants under Fe(II) and Fe(III) nutrient supply, and the normal Fe deficiency response for both strategy I and II marker genes in the leaves but not the roots indicate a defect in Fe homeostasis. In addition, root development was also compromised in Osspr1 mutant plants. It is likely that the defective roots of Osspr1 plants will have a secondary effect on nutrient uptake. However, if reduced Fe uptake as a result of damaged roots was the primary reason for the reduced Fe content in the leaves, the Fe content and the Fe deficiency response would have been duced and activated, respectively, in the roots. However, the Fe content in roots of Osspr1 mutant plants was similar to that of wild-type plants (Fig. 6e). Also, no Fe deficiency response was observed in roots (Fig. 7). However, a lower Fe content was observed in the mutant leaves, which was inconsistent with the induction of Fe-responsive genes (Figs 6c, 7). These results indicate that OsSPR1 plays a role in maintaining Fe (and other metal) homeostasis between roots and leaves.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The expression pattern of OsSPR1 was determined usingt he GUS gene driven by the OsSPR1 promoter transferred into wild-type plants. GUS staining in transgenic plants indicated that OsSPR1 was ubiquitously expressed in all plant organs, including the root, leaf, stem and spikelet (Fig. 3a–r). OsSPR1 was expressed in primary and lateral roots, both in mature regions and in root tips. Strong expression was observed in the root tip (in the meristem and lateral root initiation regions), where dividing cells are present. The expression of OsSPR1 in the root is mainly associated with vascular tissues (Fig. 3b,d,h). Semiquantitative RT-PCR analysis using SPR1-specific primers showed that SPR1 was expressed at relatively similar levelsin all tissues, including the root, stem base, stem, leaf and panicles (Fig. S3), consistent with the GUS staining patterns observed.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> Root elongation is determined by two successive processes: cell elongation and cell proliferation (Beemster et al., 2003). In rice, several genes controlling division and differentiation in root growth and development have been identified through genetic screens (Rebouillat et al., 2009). Here, we cloned OsSPR1, a novel mitochondrial gene involved in root cell elongation. Loss of function of OsSPR1 resulted in defective root elongation, including primary root, adventitious root and lateral root elongation; notably, the defects were more severe in lateral roots and adventitious roots. Analysis of the different root zones revealed similar arrangements of cells in those zones compared with the wild type, but reduced cell length was observed in the spr1 roots,<br /> suggesting that reduced root length in spr1 resulted from reduced cell elongation.<br /> <br /> ==References==<br /> Please input cited references here.<br /> Beemster GTS, Fiorani F, Inze´ D. 2003. Cell cycle: the key to plant growth control? Trends in Plant Science 8: 154–158.<br /> Rebouillat J, Dievart A, Verdeil J, Escoute J, Giese G, Breitler J, Gantet P, Espeout S, Guiderdoni E, Pe´rin C. 2009. Molecular genetics of rice root development. Rice 2: 15–34.<br /> Liqiang Jia;Zhongchang Wu;Xi Hao;Chris Carrie;Libin Zheng;James Whelan;Yunrong Wu;Shoufeng Wang;Ping Wu;Chuanzao Mao. Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa New Phytologist, 2011, 189(3): 843-855.<br /> Jia LiQiang . Is Reactive Oxygen Species (ROS) the underlying factor for inhibited root growth in Osspr1? Plant Signaling &amp; Behavior, 2011, 6(7): 1024-1025.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0898300|<br /> Description = Armadillo-like helical domain containing protein|<br /> Version = NM_001051627.1 GI:115441624 GeneID:4325109|<br /> Length = 6243 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0898300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:40808134..40814376|<br /> CDS = 40808350..40808496,40808589..40808780,40808893..40809024,40809123..40809260,40809362..40809562&lt;br&gt;,40809661..40809788,40810023..40810098,40810453..40810602,40810698..40811057&lt;br&gt;,40811144..40811296,40811384..40811676,40811792..40811988,40812071..40812228&lt;br&gt;,40812533..40812580,40812912..40813040,40813123..40813237,40813321..40813436&lt;br&gt;,40813737..40813811,40813982..40814116|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaaaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaaatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgatag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MGVMSRRVLPACSSLCYFCPSLRARSRQPVKRYKKIIAEIYQLP PDGEPNDRRIGKLCDYVSRNPTRIPKITEYLEERCYKDLRHENFTLAKVVPCIYRKLL CSCKDHTPLLATSTLSIIRTLLDQRMNDDLRVLGCLMLVDFLNGQVDSTHMFNLEGLI PKLCQISQELREDDKGFRLRCAALQALASMVQYMGDHSHISMELDEVVSVIVSCYEVN QTLSIKEVVRLQDDDDLVINGSLTGLPVSGQNSAKVASDTMSASENPAHWARVCLRNM ASIAKEATTVWRVLDPLFRLFDSHNYWSPENGIAFSILQEMQALMDKSGQNGHLLLSF TIKHIDHKSVAKKPAKQTSILKVASLLAKHAKLKASVTIASATSDLIKHLRKCMHCAV ESPNAQNDVDKWNSALYVALEECLVQLTEKVGDVGPVLDMVGVMLENLSCTATIARTT ISSVFRTVQIAASIHKSLYNQKAFPEALFHQLLLAMMHPDKKTRVGSHRVLSTIIAPS LLCPWSGISFPIPVKGNDSQSITLLALSAFSSEAVMDEVRIKSRTHEQLQNNVKPETV VGSENGYTHTEPNSRKSPGLGIPLKDENLKFMKLNSSQLVLLLSSIWSQAPLEDNSPA NFEAMCHTYNIALLCSMTKSSSHAALVRCFQLAFSLRRMSLNQENGLQPSRRRCLYTM ASAMLIFSAKVADIPQTIPLVKAAVPEKMVDPHLCLIDDCRLVISSPQSSNSGIVYGS EEDESDARNFLSCVNKNDTQLKEIVISHFKEKFENLSEKFNGIEEQLLQEFSLDDSFP LSAPLFMETPHSCSMYAEKDDHCFDEEVIPCEMDDDDDIVFEHSGSQSDRKTSGSMAS SDVLNVNQLIESVHETARQVANAPVSANLVPYDQMKSQCEALVMEKQQKMSVLLSFKH SRTDSRGSTAENGLETNESSARSEPETQSTRKERMRRSDSASSESDRSFRLPPASPYD KFMRAAGR&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5881..6027#5597..5788#5353..5484#5117..5254#4815..5015#4589..4716#4279..4354#3775..3924#3320..3679#3081..3233#2701..2993#2389..2585#2149..2306#1797..1844#1337..1465#1140..1254#941..1056#566..640#261..395#gaggtttgatcttggtttcgatccgaagggtctcggttggctgcgtgggaagggggaggaggaagaaggcgacctgctatctaatcctctaccccctctgctgcgaaaattctctgctgtttgctcaagcatcttgctgcttggagccttggatcgaagagagttttagttcttggagttctgagaattgtgttgcttgagccgaattcgatcccaaaaaggctcttttttgggaggctttgaggaggaggaggaagaagatgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggtatggcgattactgaatcctgtcttggcatttcagccatttctgatgcttagttgtagtctctgcgatttgggtctgtggtcaaatagttctagtttctgcgaattggagtctgtggtcaaatgtgtggagggagtttgttgattgagggtgtgatgttgttgctgtaggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaaggtaacacattttgatctcctctctgtttttactagaattgtcgcgcaatctgttagcttgaatagtttagactgaatttccagtatttctgaaagcagtctttataggtaccgagtagttaccacataatggctgatttacactgcattgctttagtgtgatggtgattccttttgccagagtacaaccgatcaactttcgctagcaccgatcactagctggaatgattttaggctgtttaagtaggacttaccgagatgaatgaatttgtggaactcggaactgattttatgtctgcagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacgtaagtgtaaaggctctattctgccagtcaagcacccatagcaaatgtttgctgctgtactgaccatattattcttgattcagaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttattgacgactgtaacaaattgcacctacttggtttgctagaaatctgccatctgtttggtttaatttgagttcatgtaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtaagaaaagtttttacttgattctctgatgaaatggataaggtagtcttatatgttgcgagctagccatttagggctaatttacagtagatttgttggaccagaaataataatgtagaacaaaataaggaactacattcaactattatacaatttgttttgcaaatataacatttttttaatgttggttatattgatattgcttgagaattcccaacagataatcctatacttgcattactggttcttgccaactgatgccattaaatcagtctattgattaaatttaactttttaatcctaatatactcatttaccactttgtaatcaggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagtaagtccttatcactctgtgttgggctagaatgttaagagttaagacttaattctcattcatggttgatagatgcctcctggtgaatatttgttctcttgttggaattgcataagattcatctttggagcatagacaagttgatgccattaatactacagatagacttgttgaataagtggaagatatttgattagtctatgaatagaaaaaaaaagtagcatgatagtttcatttgaagtgcgccagaaacagtaattcagtaagggttctgattagtctctgaattcattcttgaacccaggttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtgagaatttctgctaatgtaacggcccatttccacactcgatttgtgcccttgtcatcttaatgacttagtgtgttgacaggtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcaggtgcaaaatttttcacccatgctatttgtttctctttttgtgccattttttttggttttgttagtgtttaacagcagaaagatttagtgctcttctaaatcttgtttcttaacagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttcattatgctgtattattcctttgttacattactacattttgctttcatcagagagacattaaaggctgttaattcattgaataggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcaagttttctagtgtgttcaccattgcaggagaattttgctattgaacaaaagtgttaattggcaattccttgttaatatttcaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaagtatgtactccaactagtattcttattgaacttggtattttctggtcttggtgttttcggatgtccagctgacacattttcttccactgaagcagaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaaggtaggtcacttaatctagttcattcttgttatacaataggcaaaaagaattgttcaagtaacttgacttaacattgttctgcatgccttgaaattatcaaagaatagccaaagggaaagataccgtcttttcccttttggcatggagttatttcatattatataatatttttttatactctatttttacacaagatagattctttagaatattttattgatgtaataggcatggattaacactttttttaagatttccctgctagtgtgagtttgactagtgatgtcaacttctcctttggacttgaaattgtgtgctatatttgtgagattgaccattgaattttcgttgcagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaagtaagtttatcctcatttgcctgctctttaaagtttttgcttttgtatatcaaaatgatgatgcaaaaaaaaagtgacaactagctagggattttttccaaataggaatatcattttttctaataccagtatattttagccattgcgtccaacttcaagtttattatatttcctatcaatctagtcatttgatacctgcttgatttattcttacattttctactgtaaataaagatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggttagttgattcctgattttgaatatgcttgctctatatatatctctaatgtgacatttttcaatttactctgaattcgcttattgatgtttttgtaggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagaggtatacattcagatttacattatgagagcgtgttttcttgatttctctgttcacaatcaagaagttatttgacgattaagtgtcttcactgcaaattgcagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggtccgtactgattctctatagttaggtgattttttgttatttatgaattgctgttgtattaacttttttggttgcatctgcgtcccttgtgcttccaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggtatgcgccatttctgtctacaacaatggcacatgaagatcactcgctattttctagatctcttacagtcccctcatccgtttgtaatcaaacgacttttttttttcttcaggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgaggcatgttcatcttgacaggaaaaaaaaaatctatccatcactactgaaatctttgaaaatgtcctgaaaaactcatcaacatcttgttgcagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgataggctggtgcaatgcaatgcgatggcgccaaggatctgatactagttttcaagcctgtatacagaaatgtttttacagatacaagtcttctaaagctgtttcttttcattgtcgccttgtattttttattcttctgagtgtaaatttctcgagaaggtgtacaaatttctgttactgttgtagagacccagaataaataaaagtataggcattggtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051627.1 RefSeq:Os01g0898300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0898300&diff=176623 2014-06-03T10:06:08Z

<p>Wuxt: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study has identified a novel mitochondrial protein, OsSPR1, that is involved in maintaining Fe homeostasis in rice. The large reduction of Fe content observed in the leaves but not the roots of Osspr1 mutant plants under Fe(II) and Fe(III) nutrient supply, and the normal Fe deficiency response for both strategy I and II marker genes in the leaves but not the roots indicate a defect in Fe homeostasis. In addition, root development was also compromised in Osspr1 mutant plants. It is likely that the defective roots of Osspr1 plants will have a secondary effect on nutrient uptake. However, if reduced Fe uptake as a result of damaged roots was the primary reason for the reduced Fe content in the leaves, the Fe content and the Fe deficiency response would have been duced and activated, respectively, in the roots. However, the Fe content in roots of Osspr1 mutant plants was similar to that of wild-type plants (Fig. 6e). Also, no Fe deficiency response was observed in roots (Fig. 7). However, a lower Fe content was observed in the mutant leaves, which was inconsistent with the induction of Fe-responsive genes (Figs 6c, 7). These results indicate that OsSPR1 plays a role in maintaining Fe (and other metal) homeostasis between roots and leaves.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The expression pattern of OsSPR1 was determined usingt he GUS gene driven by the OsSPR1 promoter transferred into wild-type plants. GUS staining in transgenic plants indicated that OsSPR1 was ubiquitously expressed in all plant organs, including the root, leaf, stem and spikelet (Fig. 3a–r). OsSPR1 was expressed in primary and lateral roots, both in mature regions and in root tips. Strong expression was observed in the root tip (in the meristem and lateral root initiation regions), where dividing cells are present. The expression of OsSPR1 in the root is mainly associated with vascular tissues (Fig. 3b,d,h). Semiquantitative RT-PCR analysis using SPR1-specific primers showed that SPR1 was expressed at relatively similar levelsin all tissues, including the root, stem base, stem, leaf and panicles (Fig. S3), consistent with the GUS staining patterns observed.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> Root elongation is determined by two successive processes: cell elongation and cell proliferation (Beemster et al., 2003). In rice, several genes controlling division and differentiation in root growth and development have been identified through genetic screens (Rebouillat et al., 2009). Here, we cloned OsSPR1, a novel mitochondrial gene involved in root cell elongation. Loss of function of OsSPR1 resulted in defective root elongation, including primary root, adventitious root and lateral root elongation; notably, the defects were more severe in lateral roots and adventitious roots. Analysis of the different root zones revealed similar arrangements of cells in those zones compared with the wild type, but reduced cell length was observed in the spr1 roots,<br /> suggesting that reduced root length in spr1 resulted from reduced cell elongation.<br /> <br /> 1.Beemster GTS, Fiorani F, Inze´ D. 2003. Cell cycle: the key to plant growth control? Trends in Plant Science 8: 154–158.<br /> <br /> 2.Rebouillat J, Dievart A, Verdeil J, Escoute J, Giese G, Breitler J, Gantet P, Espeout S, Guiderdoni E, Pe´rin C. 2009. Molecular genetics of rice root development. Rice 2: 15–34.<br /> <br /> 3.Liqiang Jia;Zhongchang Wu;Xi Hao;Chris Carrie;Libin Zheng;James Whelan;Yunrong Wu;Shoufeng Wang;Ping Wu;Chuanzao Mao. Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa New Phytologist, 2011, 189(3): 843-855.<br /> <br /> 4.Jia LiQiang . Is Reactive Oxygen Species (ROS) the underlying factor for inhibited root growth in Osspr1? Plant Signaling &amp; Behavior, 2011, 6(7): 1024-1025.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0898300|<br /> Description = Armadillo-like helical domain containing protein|<br /> Version = NM_001051627.1 GI:115441624 GeneID:4325109|<br /> Length = 6243 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0898300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:40808134..40814376|<br /> CDS = 40808350..40808496,40808589..40808780,40808893..40809024,40809123..40809260,40809362..40809562&lt;br&gt;,40809661..40809788,40810023..40810098,40810453..40810602,40810698..40811057&lt;br&gt;,40811144..40811296,40811384..40811676,40811792..40811988,40812071..40812228&lt;br&gt;,40812533..40812580,40812912..40813040,40813123..40813237,40813321..40813436&lt;br&gt;,40813737..40813811,40813982..40814116|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaaaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaaatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgatag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MGVMSRRVLPACSSLCYFCPSLRARSRQPVKRYKKIIAEIYQLP PDGEPNDRRIGKLCDYVSRNPTRIPKITEYLEERCYKDLRHENFTLAKVVPCIYRKLL CSCKDHTPLLATSTLSIIRTLLDQRMNDDLRVLGCLMLVDFLNGQVDSTHMFNLEGLI PKLCQISQELREDDKGFRLRCAALQALASMVQYMGDHSHISMELDEVVSVIVSCYEVN QTLSIKEVVRLQDDDDLVINGSLTGLPVSGQNSAKVASDTMSASENPAHWARVCLRNM ASIAKEATTVWRVLDPLFRLFDSHNYWSPENGIAFSILQEMQALMDKSGQNGHLLLSF TIKHIDHKSVAKKPAKQTSILKVASLLAKHAKLKASVTIASATSDLIKHLRKCMHCAV ESPNAQNDVDKWNSALYVALEECLVQLTEKVGDVGPVLDMVGVMLENLSCTATIARTT ISSVFRTVQIAASIHKSLYNQKAFPEALFHQLLLAMMHPDKKTRVGSHRVLSTIIAPS LLCPWSGISFPIPVKGNDSQSITLLALSAFSSEAVMDEVRIKSRTHEQLQNNVKPETV VGSENGYTHTEPNSRKSPGLGIPLKDENLKFMKLNSSQLVLLLSSIWSQAPLEDNSPA NFEAMCHTYNIALLCSMTKSSSHAALVRCFQLAFSLRRMSLNQENGLQPSRRRCLYTM ASAMLIFSAKVADIPQTIPLVKAAVPEKMVDPHLCLIDDCRLVISSPQSSNSGIVYGS EEDESDARNFLSCVNKNDTQLKEIVISHFKEKFENLSEKFNGIEEQLLQEFSLDDSFP LSAPLFMETPHSCSMYAEKDDHCFDEEVIPCEMDDDDDIVFEHSGSQSDRKTSGSMAS SDVLNVNQLIESVHETARQVANAPVSANLVPYDQMKSQCEALVMEKQQKMSVLLSFKH SRTDSRGSTAENGLETNESSARSEPETQSTRKERMRRSDSASSESDRSFRLPPASPYD KFMRAAGR&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5881..6027#5597..5788#5353..5484#5117..5254#4815..5015#4589..4716#4279..4354#3775..3924#3320..3679#3081..3233#2701..2993#2389..2585#2149..2306#1797..1844#1337..1465#1140..1254#941..1056#566..640#261..395#gaggtttgatcttggtttcgatccgaagggtctcggttggctgcgtgggaagggggaggaggaagaaggcgacctgctatctaatcctctaccccctctgctgcgaaaattctctgctgtttgctcaagcatcttgctgcttggagccttggatcgaagagagttttagttcttggagttctgagaattgtgttgcttgagccgaattcgatcccaaaaaggctcttttttgggaggctttgaggaggaggaggaagaagatgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggtatggcgattactgaatcctgtcttggcatttcagccatttctgatgcttagttgtagtctctgcgatttgggtctgtggtcaaatagttctagtttctgcgaattggagtctgtggtcaaatgtgtggagggagtttgttgattgagggtgtgatgttgttgctgtaggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaaggtaacacattttgatctcctctctgtttttactagaattgtcgcgcaatctgttagcttgaatagtttagactgaatttccagtatttctgaaagcagtctttataggtaccgagtagttaccacataatggctgatttacactgcattgctttagtgtgatggtgattccttttgccagagtacaaccgatcaactttcgctagcaccgatcactagctggaatgattttaggctgtttaagtaggacttaccgagatgaatgaatttgtggaactcggaactgattttatgtctgcagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacgtaagtgtaaaggctctattctgccagtcaagcacccatagcaaatgtttgctgctgtactgaccatattattcttgattcagaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttattgacgactgtaacaaattgcacctacttggtttgctagaaatctgccatctgtttggtttaatttgagttcatgtaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtaagaaaagtttttacttgattctctgatgaaatggataaggtagtcttatatgttgcgagctagccatttagggctaatttacagtagatttgttggaccagaaataataatgtagaacaaaataaggaactacattcaactattatacaatttgttttgcaaatataacatttttttaatgttggttatattgatattgcttgagaattcccaacagataatcctatacttgcattactggttcttgccaactgatgccattaaatcagtctattgattaaatttaactttttaatcctaatatactcatttaccactttgtaatcaggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagtaagtccttatcactctgtgttgggctagaatgttaagagttaagacttaattctcattcatggttgatagatgcctcctggtgaatatttgttctcttgttggaattgcataagattcatctttggagcatagacaagttgatgccattaatactacagatagacttgttgaataagtggaagatatttgattagtctatgaatagaaaaaaaaagtagcatgatagtttcatttgaagtgcgccagaaacagtaattcagtaagggttctgattagtctctgaattcattcttgaacccaggttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtgagaatttctgctaatgtaacggcccatttccacactcgatttgtgcccttgtcatcttaatgacttagtgtgttgacaggtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcaggtgcaaaatttttcacccatgctatttgtttctctttttgtgccattttttttggttttgttagtgtttaacagcagaaagatttagtgctcttctaaatcttgtttcttaacagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttcattatgctgtattattcctttgttacattactacattttgctttcatcagagagacattaaaggctgttaattcattgaataggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcaagttttctagtgtgttcaccattgcaggagaattttgctattgaacaaaagtgttaattggcaattccttgttaatatttcaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaagtatgtactccaactagtattcttattgaacttggtattttctggtcttggtgttttcggatgtccagctgacacattttcttccactgaagcagaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaaggtaggtcacttaatctagttcattcttgttatacaataggcaaaaagaattgttcaagtaacttgacttaacattgttctgcatgccttgaaattatcaaagaatagccaaagggaaagataccgtcttttcccttttggcatggagttatttcatattatataatatttttttatactctatttttacacaagatagattctttagaatattttattgatgtaataggcatggattaacactttttttaagatttccctgctagtgtgagtttgactagtgatgtcaacttctcctttggacttgaaattgtgtgctatatttgtgagattgaccattgaattttcgttgcagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaagtaagtttatcctcatttgcctgctctttaaagtttttgcttttgtatatcaaaatgatgatgcaaaaaaaaagtgacaactagctagggattttttccaaataggaatatcattttttctaataccagtatattttagccattgcgtccaacttcaagtttattatatttcctatcaatctagtcatttgatacctgcttgatttattcttacattttctactgtaaataaagatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggttagttgattcctgattttgaatatgcttgctctatatatatctctaatgtgacatttttcaatttactctgaattcgcttattgatgtttttgtaggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagaggtatacattcagatttacattatgagagcgtgttttcttgatttctctgttcacaatcaagaagttatttgacgattaagtgtcttcactgcaaattgcagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggtccgtactgattctctatagttaggtgattttttgttatttatgaattgctgttgtattaacttttttggttgcatctgcgtcccttgtgcttccaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggtatgcgccatttctgtctacaacaatggcacatgaagatcactcgctattttctagatctcttacagtcccctcatccgtttgtaatcaaacgacttttttttttcttcaggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgaggcatgttcatcttgacaggaaaaaaaaaatctatccatcactactgaaatctttgaaaatgtcctgaaaaactcatcaacatcttgttgcagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgataggctggtgcaatgcaatgcgatggcgccaaggatctgatactagttttcaagcctgtatacagaaatgtttttacagatacaagtcttctaaagctgtttcttttcattgtcgccttgtattttttattcttctgagtgtaaatttctcgagaaggtgtacaaatttctgttactgttgtagagacccagaataaataaaagtataggcattggtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051627.1 RefSeq:Os01g0898300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0898300&diff=176043 2014-06-02T06:25:38Z

<p>Wuxt: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study has identified a novel mitochondrial protein, OsSPR1, that is involved in maintaining Fe homeostasis in rice. The large reduction of Fe content observed in the leaves but not the roots of Osspr1 mutant plants under Fe(II) and Fe(III) nutrient supply, and the normal Fe deficiency response for both strategy I and II marker genes in the leaves but not the roots indicate a defect in Fe homeostasis. In addition, root development was also compromised in Osspr1 mutant plants. It is likely that the defective roots of Osspr1 plants will have a secondary effect on nutrient uptake. However, if reduced Fe uptake as a result of damaged roots was the primary reason for the reduced Fe content in the leaves, the Fe content and the Fe deficiency response would have been duced and activated, respectively, in the roots. However, the Fe content in roots of Osspr1 mutant plants was similar to that of wild-type plants (Fig. 6e). Also, no Fe deficiency response was observed in roots (Fig. 7). However, a lower Fe content was observed in the mutant leaves, which was inconsistent with the induction of Fe-responsive genes (Figs 6c, 7). These results indicate that OsSPR1 plays a role in maintaining Fe (and other metal) homeostasis between roots and leaves.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The expression pattern of OsSPR1 was determined usingt he GUS gene driven by the OsSPR1 promoter transferred into wild-type plants. GUS staining in transgenic plants indicated that OsSPR1 was ubiquitously expressed in all plant organs, including the root, leaf, stem and spikelet (Fig. 3a–r). OsSPR1 was expressed in primary and lateral roots, both in mature regions and in root tips. Strong expression was observed in the root tip (in the meristem and lateral root initiation regions), where dividing cells are present. The expression of OsSPR1 in the root is mainly associated with vascular tissues (Fig. 3b,d,h). Semiquantitative RT-PCR analysis using SPR1-specific primers showed that SPR1 was expressed at relatively similar levelsin all tissues, including the root, stem base, stem, leaf and panicles (Fig. S3), consistent with the GUS staining patterns observed.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> Root elongation is determined by two successive processes: cell elongation and cell proliferation (Beemster et al., 2003). In rice, several genes controlling division and differentiation in root growth and development have been identified through genetic screens (Rebouillat et al., 2009). Here, we cloned OsSPR1, a novel mitochondrial gene involved in root cell elongation. Loss of function of OsSPR1 resulted in defective root elongation, including primary root, adventitious root and lateral root elongation; notably, the defects were more severe in lateral roots and adventitious roots. Analysis of the different root zones revealed similar arrangements of cells in those zones compared with the wild type, but reduced cell length was observed in the spr1 roots,<br /> suggesting that reduced root length in spr1 resulted from reduced cell elongation.<br /> <br /> ==References==<br /> Please input cited references here.<br /> Beemster GTS, Fiorani F, Inze´ D. 2003. Cell cycle: the key to plant growth control? Trends in Plant Science 8: 154–158.<br /> Rebouillat J, Dievart A, Verdeil J, Escoute J, Giese G, Breitler J, Gantet P, Espeout S, Guiderdoni E, Pe´rin C. 2009. Molecular genetics of rice root development. Rice 2: 15–34.<br /> Liqiang Jia;Zhongchang Wu;Xi Hao;Chris Carrie;Libin Zheng;James Whelan;Yunrong Wu;Shoufeng Wang;Ping Wu;Chuanzao Mao. Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa New Phytologist, 2011, 189(3): 843-855.<br /> Jia LiQiang . Is Reactive Oxygen Species (ROS) the underlying factor for inhibited root growth in Osspr1? Plant Signaling &amp; Behavior, 2011, 6(7): 1024-1025.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0898300|<br /> Description = Armadillo-like helical domain containing protein|<br /> Version = NM_001051627.1 GI:115441624 GeneID:4325109|<br /> Length = 6243 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0898300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:40808134..40814376|<br /> CDS = 40808350..40808496,40808589..40808780,40808893..40809024,40809123..40809260,40809362..40809562&lt;br&gt;,40809661..40809788,40810023..40810098,40810453..40810602,40810698..40811057&lt;br&gt;,40811144..40811296,40811384..40811676,40811792..40811988,40812071..40812228&lt;br&gt;,40812533..40812580,40812912..40813040,40813123..40813237,40813321..40813436&lt;br&gt;,40813737..40813811,40813982..40814116|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaaaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaaatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgatag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MGVMSRRVLPACSSLCYFCPSLRARSRQPVKRYKKIIAEIYQLP PDGEPNDRRIGKLCDYVSRNPTRIPKITEYLEERCYKDLRHENFTLAKVVPCIYRKLL CSCKDHTPLLATSTLSIIRTLLDQRMNDDLRVLGCLMLVDFLNGQVDSTHMFNLEGLI PKLCQISQELREDDKGFRLRCAALQALASMVQYMGDHSHISMELDEVVSVIVSCYEVN QTLSIKEVVRLQDDDDLVINGSLTGLPVSGQNSAKVASDTMSASENPAHWARVCLRNM ASIAKEATTVWRVLDPLFRLFDSHNYWSPENGIAFSILQEMQALMDKSGQNGHLLLSF TIKHIDHKSVAKKPAKQTSILKVASLLAKHAKLKASVTIASATSDLIKHLRKCMHCAV ESPNAQNDVDKWNSALYVALEECLVQLTEKVGDVGPVLDMVGVMLENLSCTATIARTT ISSVFRTVQIAASIHKSLYNQKAFPEALFHQLLLAMMHPDKKTRVGSHRVLSTIIAPS LLCPWSGISFPIPVKGNDSQSITLLALSAFSSEAVMDEVRIKSRTHEQLQNNVKPETV VGSENGYTHTEPNSRKSPGLGIPLKDENLKFMKLNSSQLVLLLSSIWSQAPLEDNSPA NFEAMCHTYNIALLCSMTKSSSHAALVRCFQLAFSLRRMSLNQENGLQPSRRRCLYTM ASAMLIFSAKVADIPQTIPLVKAAVPEKMVDPHLCLIDDCRLVISSPQSSNSGIVYGS EEDESDARNFLSCVNKNDTQLKEIVISHFKEKFENLSEKFNGIEEQLLQEFSLDDSFP LSAPLFMETPHSCSMYAEKDDHCFDEEVIPCEMDDDDDIVFEHSGSQSDRKTSGSMAS SDVLNVNQLIESVHETARQVANAPVSANLVPYDQMKSQCEALVMEKQQKMSVLLSFKH SRTDSRGSTAENGLETNESSARSEPETQSTRKERMRRSDSASSESDRSFRLPPASPYD KFMRAAGR&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5881..6027#5597..5788#5353..5484#5117..5254#4815..5015#4589..4716#4279..4354#3775..3924#3320..3679#3081..3233#2701..2993#2389..2585#2149..2306#1797..1844#1337..1465#1140..1254#941..1056#566..640#261..395#gaggtttgatcttggtttcgatccgaagggtctcggttggctgcgtgggaagggggaggaggaagaaggcgacctgctatctaatcctctaccccctctgctgcgaaaattctctgctgtttgctcaagcatcttgctgcttggagccttggatcgaagagagttttagttcttggagttctgagaattgtgttgcttgagccgaattcgatcccaaaaaggctcttttttgggaggctttgaggaggaggaggaagaagatgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggtatggcgattactgaatcctgtcttggcatttcagccatttctgatgcttagttgtagtctctgcgatttgggtctgtggtcaaatagttctagtttctgcgaattggagtctgtggtcaaatgtgtggagggagtttgttgattgagggtgtgatgttgttgctgtaggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaaggtaacacattttgatctcctctctgtttttactagaattgtcgcgcaatctgttagcttgaatagtttagactgaatttccagtatttctgaaagcagtctttataggtaccgagtagttaccacataatggctgatttacactgcattgctttagtgtgatggtgattccttttgccagagtacaaccgatcaactttcgctagcaccgatcactagctggaatgattttaggctgtttaagtaggacttaccgagatgaatgaatttgtggaactcggaactgattttatgtctgcagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacgtaagtgtaaaggctctattctgccagtcaagcacccatagcaaatgtttgctgctgtactgaccatattattcttgattcagaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttattgacgactgtaacaaattgcacctacttggtttgctagaaatctgccatctgtttggtttaatttgagttcatgtaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtaagaaaagtttttacttgattctctgatgaaatggataaggtagtcttatatgttgcgagctagccatttagggctaatttacagtagatttgttggaccagaaataataatgtagaacaaaataaggaactacattcaactattatacaatttgttttgcaaatataacatttttttaatgttggttatattgatattgcttgagaattcccaacagataatcctatacttgcattactggttcttgccaactgatgccattaaatcagtctattgattaaatttaactttttaatcctaatatactcatttaccactttgtaatcaggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagtaagtccttatcactctgtgttgggctagaatgttaagagttaagacttaattctcattcatggttgatagatgcctcctggtgaatatttgttctcttgttggaattgcataagattcatctttggagcatagacaagttgatgccattaatactacagatagacttgttgaataagtggaagatatttgattagtctatgaatagaaaaaaaaagtagcatgatagtttcatttgaagtgcgccagaaacagtaattcagtaagggttctgattagtctctgaattcattcttgaacccaggttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtgagaatttctgctaatgtaacggcccatttccacactcgatttgtgcccttgtcatcttaatgacttagtgtgttgacaggtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcaggtgcaaaatttttcacccatgctatttgtttctctttttgtgccattttttttggttttgttagtgtttaacagcagaaagatttagtgctcttctaaatcttgtttcttaacagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttcattatgctgtattattcctttgttacattactacattttgctttcatcagagagacattaaaggctgttaattcattgaataggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcaagttttctagtgtgttcaccattgcaggagaattttgctattgaacaaaagtgttaattggcaattccttgttaatatttcaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaagtatgtactccaactagtattcttattgaacttggtattttctggtcttggtgttttcggatgtccagctgacacattttcttccactgaagcagaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaaggtaggtcacttaatctagttcattcttgttatacaataggcaaaaagaattgttcaagtaacttgacttaacattgttctgcatgccttgaaattatcaaagaatagccaaagggaaagataccgtcttttcccttttggcatggagttatttcatattatataatatttttttatactctatttttacacaagatagattctttagaatattttattgatgtaataggcatggattaacactttttttaagatttccctgctagtgtgagtttgactagtgatgtcaacttctcctttggacttgaaattgtgtgctatatttgtgagattgaccattgaattttcgttgcagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaagtaagtttatcctcatttgcctgctctttaaagtttttgcttttgtatatcaaaatgatgatgcaaaaaaaaagtgacaactagctagggattttttccaaataggaatatcattttttctaataccagtatattttagccattgcgtccaacttcaagtttattatatttcctatcaatctagtcatttgatacctgcttgatttattcttacattttctactgtaaataaagatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggttagttgattcctgattttgaatatgcttgctctatatatatctctaatgtgacatttttcaatttactctgaattcgcttattgatgtttttgtaggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagaggtatacattcagatttacattatgagagcgtgttttcttgatttctctgttcacaatcaagaagttatttgacgattaagtgtcttcactgcaaattgcagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggtccgtactgattctctatagttaggtgattttttgttatttatgaattgctgttgtattaacttttttggttgcatctgcgtcccttgtgcttccaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggtatgcgccatttctgtctacaacaatggcacatgaagatcactcgctattttctagatctcttacagtcccctcatccgtttgtaatcaaacgacttttttttttcttcaggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgaggcatgttcatcttgacaggaaaaaaaaaatctatccatcactactgaaatctttgaaaatgtcctgaaaaactcatcaacatcttgttgcagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgataggctggtgcaatgcaatgcgatggcgccaaggatctgatactagttttcaagcctgtatacagaaatgtttttacagatacaagtcttctaaagctgtttcttttcattgtcgccttgtattttttattcttctgagtgtaaatttctcgagaaggtgtacaaatttctgttactgttgtagagacccagaataaataaaagtataggcattggtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051627.1 RefSeq:Os01g0898300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0898300&diff=176042 2014-06-02T06:23:55Z

<p>Wuxt: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study has identified a novel mitochondrial protein, OsSPR1, that is involved in maintaining Fe homeostasis in rice. The large reduction of Fe content observed in the leaves but not the roots of Osspr1 mutant plants under Fe(II) and Fe(III) nutrient supply, and the normal Fe deficiency response for both strategy I and II marker genes in the leaves but not the roots indicate a defect in Fe homeostasis. In addition, root development was also compromised in Osspr1 mutant plants. It is likely that the defective roots of Osspr1 plants will have a secondary effect on nutrient uptake. However, if reduced Fe uptake as a result of damaged roots was the primary reason for the reduced Fe content in the leaves, the Fe content and the Fe deficiency response would have been duced and activated, respectively, in the roots. However, the Fe content in roots of Osspr1 mutant plants was similar to that of wild-type plants (Fig. 6e). Also, no Fe deficiency response was observed in roots (Fig. 7). However, a lower Fe content was observed in the mutant leaves, which was inconsistent with the induction of Fe-responsive genes (Figs 6c, 7). These results indicate that OsSPR1 plays a role in maintaining Fe (and other metal) homeostasis between roots and leaves.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The expression pattern of OsSPR1 was determined usingt he GUS gene driven by the OsSPR1 promoter transferred into wild-type plants. GUS staining in transgenic plants indicated that OsSPR1 was ubiquitously expressed in all plant organs, including the root, leaf, stem and spikelet (Fig. 3a–r). OsSPR1 was expressed in primary and lateral roots, both in mature regions and in root tips. Strong expression was observed in the root tip (in the meristem and lateral root initiation regions), where dividing cells are present. The expression of OsSPR1 in the root is mainly associated with vascular tissues (Fig. 3b,d,h). Semiquantitative RT-PCR analysis using SPR1-specific primers showed that SPR1 was expressed at relatively similar levelsin all tissues, including the root, stem base, stem, leaf and panicles (Fig. S3), consistent with the GUS staining patterns observed.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> Root elongation is determined by two successive processes: cell elongation and cell proliferation (Beemster et al., 2003). In rice, several genes controlling division and differentiation in root growth and development have been identified through genetic screens (Rebouillat et al., 2009). Here, we cloned OsSPR1, a novel mitochondrial gene involved in root cell elongation. Loss of function of OsSPR1 resulted in defective root elongation, including primary root, adventitious root and lateral root elongation; notably, the defects were more severe in lateral roots and adventitious roots. Analysis of the different root zones revealed similar arrangements of cells in those zones compared with the wild type, but reduced cell length was observed in the spr1 roots,<br /> suggesting that reduced root length in spr1 resulted from reduced cell elongation.<br /> <br /> ==References==<br /> Please input cited references here.<br /> Beemster GTS, Fiorani F, Inze´ D. 2003. Cell cycle: the key to plant growth control? Trends in Plant Science 8: 154–158.<br /> Rebouillat J, Dievart A, Verdeil J, Escoute J, Giese G, Breitler J, Gantet P, Espeout S, Guiderdoni E, Pe´rin C. 2009. Molecular genetics of rice root development. Rice 2: 15–34.<br /> Liqiang Jia;Zhongchang Wu;Xi Hao;Chris Carrie;Libin Zheng;James Whelan;Yunrong Wu;Shoufeng Wang;Ping Wu;Chuanzao Mao. Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa New Phytologist, 2011, 189(3): 843-855.<br /> Jia LiQiang . Is Reactive Oxygen Species (ROS) the underlying factor for inhibited root growth in Osspr1? Plant Signaling &amp; Behavior, 2011, 6(7): 1024-1025.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0898300|<br /> Description = Armadillo-like helical domain containing protein|<br /> Version = NM_001051627.1 GI:115441624 GeneID:4325109|<br /> Length = 6243 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0898300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:40808134..40814376|<br /> CDS = 40808350..40808496,40808589..40808780,40808893..40809024,40809123..40809260,40809362..40809562&lt;br&gt;,40809661..40809788,40810023..40810098,40810453..40810602,40810698..40811057&lt;br&gt;,40811144..40811296,40811384..40811676,40811792..40811988,40812071..40812228&lt;br&gt;,40812533..40812580,40812912..40813040,40813123..40813237,40813321..40813436&lt;br&gt;,40813737..40813811,40813982..40814116|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaaaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaaatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgatag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MGVMSRRVLPACSSLCYFCPSLRARSRQPVKRYKKIIAEIYQLP PDGEPNDRRIGKLCDYVSRNPTRIPKITEYLEERCYKDLRHENFTLAKVVPCIYRKLL CSCKDHTPLLATSTLSIIRTLLDQRMNDDLRVLGCLMLVDFLNGQVDSTHMFNLEGLI PKLCQISQELREDDKGFRLRCAALQALASMVQYMGDHSHISMELDEVVSVIVSCYEVN QTLSIKEVVRLQDDDDLVINGSLTGLPVSGQNSAKVASDTMSASENPAHWARVCLRNM ASIAKEATTVWRVLDPLFRLFDSHNYWSPENGIAFSILQEMQALMDKSGQNGHLLLSF TIKHIDHKSVAKKPAKQTSILKVASLLAKHAKLKASVTIASATSDLIKHLRKCMHCAV ESPNAQNDVDKWNSALYVALEECLVQLTEKVGDVGPVLDMVGVMLENLSCTATIARTT ISSVFRTVQIAASIHKSLYNQKAFPEALFHQLLLAMMHPDKKTRVGSHRVLSTIIAPS LLCPWSGISFPIPVKGNDSQSITLLALSAFSSEAVMDEVRIKSRTHEQLQNNVKPETV VGSENGYTHTEPNSRKSPGLGIPLKDENLKFMKLNSSQLVLLLSSIWSQAPLEDNSPA NFEAMCHTYNIALLCSMTKSSSHAALVRCFQLAFSLRRMSLNQENGLQPSRRRCLYTM ASAMLIFSAKVADIPQTIPLVKAAVPEKMVDPHLCLIDDCRLVISSPQSSNSGIVYGS EEDESDARNFLSCVNKNDTQLKEIVISHFKEKFENLSEKFNGIEEQLLQEFSLDDSFP LSAPLFMETPHSCSMYAEKDDHCFDEEVIPCEMDDDDDIVFEHSGSQSDRKTSGSMAS SDVLNVNQLIESVHETARQVANAPVSANLVPYDQMKSQCEALVMEKQQKMSVLLSFKH SRTDSRGSTAENGLETNESSARSEPETQSTRKERMRRSDSASSESDRSFRLPPASPYD KFMRAAGR&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5881..6027#5597..5788#5353..5484#5117..5254#4815..5015#4589..4716#4279..4354#3775..3924#3320..3679#3081..3233#2701..2993#2389..2585#2149..2306#1797..1844#1337..1465#1140..1254#941..1056#566..640#261..395#gaggtttgatcttggtttcgatccgaagggtctcggttggctgcgtgggaagggggaggaggaagaaggcgacctgctatctaatcctctaccccctctgctgcgaaaattctctgctgtttgctcaagcatcttgctgcttggagccttggatcgaagagagttttagttcttggagttctgagaattgtgttgcttgagccgaattcgatcccaaaaaggctcttttttgggaggctttgaggaggaggaggaagaagatgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggtatggcgattactgaatcctgtcttggcatttcagccatttctgatgcttagttgtagtctctgcgatttgggtctgtggtcaaatagttctagtttctgcgaattggagtctgtggtcaaatgtgtggagggagtttgttgattgagggtgtgatgttgttgctgtaggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaaggtaacacattttgatctcctctctgtttttactagaattgtcgcgcaatctgttagcttgaatagtttagactgaatttccagtatttctgaaagcagtctttataggtaccgagtagttaccacataatggctgatttacactgcattgctttagtgtgatggtgattccttttgccagagtacaaccgatcaactttcgctagcaccgatcactagctggaatgattttaggctgtttaagtaggacttaccgagatgaatgaatttgtggaactcggaactgattttatgtctgcagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacgtaagtgtaaaggctctattctgccagtcaagcacccatagcaaatgtttgctgctgtactgaccatattattcttgattcagaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttattgacgactgtaacaaattgcacctacttggtttgctagaaatctgccatctgtttggtttaatttgagttcatgtaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtaagaaaagtttttacttgattctctgatgaaatggataaggtagtcttatatgttgcgagctagccatttagggctaatttacagtagatttgttggaccagaaataataatgtagaacaaaataaggaactacattcaactattatacaatttgttttgcaaatataacatttttttaatgttggttatattgatattgcttgagaattcccaacagataatcctatacttgcattactggttcttgccaactgatgccattaaatcagtctattgattaaatttaactttttaatcctaatatactcatttaccactttgtaatcaggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagtaagtccttatcactctgtgttgggctagaatgttaagagttaagacttaattctcattcatggttgatagatgcctcctggtgaatatttgttctcttgttggaattgcataagattcatctttggagcatagacaagttgatgccattaatactacagatagacttgttgaataagtggaagatatttgattagtctatgaatagaaaaaaaaagtagcatgatagtttcatttgaagtgcgccagaaacagtaattcagtaagggttctgattagtctctgaattcattcttgaacccaggttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtgagaatttctgctaatgtaacggcccatttccacactcgatttgtgcccttgtcatcttaatgacttagtgtgttgacaggtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcaggtgcaaaatttttcacccatgctatttgtttctctttttgtgccattttttttggttttgttagtgtttaacagcagaaagatttagtgctcttctaaatcttgtttcttaacagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttcattatgctgtattattcctttgttacattactacattttgctttcatcagagagacattaaaggctgttaattcattgaataggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcaagttttctagtgtgttcaccattgcaggagaattttgctattgaacaaaagtgttaattggcaattccttgttaatatttcaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaagtatgtactccaactagtattcttattgaacttggtattttctggtcttggtgttttcggatgtccagctgacacattttcttccactgaagcagaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaaggtaggtcacttaatctagttcattcttgttatacaataggcaaaaagaattgttcaagtaacttgacttaacattgttctgcatgccttgaaattatcaaagaatagccaaagggaaagataccgtcttttcccttttggcatggagttatttcatattatataatatttttttatactctatttttacacaagatagattctttagaatattttattgatgtaataggcatggattaacactttttttaagatttccctgctagtgtgagtttgactagtgatgtcaacttctcctttggacttgaaattgtgtgctatatttgtgagattgaccattgaattttcgttgcagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaagtaagtttatcctcatttgcctgctctttaaagtttttgcttttgtatatcaaaatgatgatgcaaaaaaaaagtgacaactagctagggattttttccaaataggaatatcattttttctaataccagtatattttagccattgcgtccaacttcaagtttattatatttcctatcaatctagtcatttgatacctgcttgatttattcttacattttctactgtaaataaagatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggttagttgattcctgattttgaatatgcttgctctatatatatctctaatgtgacatttttcaatttactctgaattcgcttattgatgtttttgtaggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagaggtatacattcagatttacattatgagagcgtgttttcttgatttctctgttcacaatcaagaagttatttgacgattaagtgtcttcactgcaaattgcagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggtccgtactgattctctatagttaggtgattttttgttatttatgaattgctgttgtattaacttttttggttgcatctgcgtcccttgtgcttccaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggtatgcgccatttctgtctacaacaatggcacatgaagatcactcgctattttctagatctcttacagtcccctcatccgtttgtaatcaaacgacttttttttttcttcaggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgaggcatgttcatcttgacaggaaaaaaaaaatctatccatcactactgaaatctttgaaaatgtcctgaaaaactcatcaacatcttgttgcagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgataggctggtgcaatgcaatgcgatggcgccaaggatctgatactagttttcaagcctgtatacagaaatgtttttacagatacaagtcttctaaagctgtttcttttcattgtcgccttgtattttttattcttctgagtgtaaatttctcgagaaggtgtacaaatttctgttactgttgtagagacccagaataaataaaagtataggcattggtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051627.1 RefSeq:Os01g0898300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0898300&diff=176041 2014-06-02T06:23:15Z

<p>Wuxt: /* References */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study has identified a novel mitochondrial protein, OsSPR1, that is involved in maintaining Fe homeostasis in rice. The large reduction of Fe content observed in the leaves but not the roots of Osspr1 mutant plants under Fe(II) and Fe(III) nutrient supply, and the normal Fe deficiency response for both strategy I and II marker genes in the leaves but not the roots indicate a defect in Fe homeostasis. In addition, root development was also compromised in Osspr1 mutant plants. It is likely that the defective roots of Osspr1 plants will have a secondary effect on nutrient uptake. However, if reduced Fe uptake as a result of damaged roots was the primary reason for the reduced Fe content in the leaves, the Fe content and the Fe deficiency response would have been duced and activated, respectively, in the roots. However, the Fe content in roots of Osspr1 mutant plants was similar to that of wild-type plants (Fig. 6e). Also, no Fe deficiency response was observed in roots (Fig. 7). However, a lower Fe content was observed in the mutant leaves, which was inconsistent with the induction of Fe-responsive genes (Figs 6c, 7). These results indicate that OsSPR1 plays a role in maintaining Fe (and other metal) homeostasis between roots and leaves.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The expression pattern of OsSPR1 was determined usingt he GUS gene driven by the OsSPR1 promoter transferred into wild-type plants. GUS staining in transgenic plants indicated that OsSPR1 was ubiquitously expressed in all plant organs, including the root, leaf, stem and spikelet (Fig. 3a–r). OsSPR1 was expressed in primary and lateral roots, both in mature regions and in root tips. Strong expression was observed in the root tip (in the meristem and lateral root initiation regions), where dividing cells are present. The expression of OsSPR1 in the root is mainly associated with vascular tissues (Fig. 3b,d,h). Semiquantitative RT-PCR analysis using SPR1-specific primers showed that SPR1 was expressed at relatively similar levelsin all tissues, including the root, stem base, stem, leaf and panicles (Fig. S3), consistent with the GUS staining patterns observed.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> Root elongation is determined by two successive processes: cell elongation and cell proliferation (Beemster et al., 2003). In rice, several genes controlling division and differentiation in root growth and development have been identified through genetic screens (Rebouillat et al., 2009). Here, we cloned OsSPR1, a novel mitochondrial gene involved in root cell elongation. Loss of function of OsSPR1 resulted in defective root elongation, including primary root, adventitious root and lateral root elongation; notably, the defects were more severe in lateral roots and adventitious roots. Analysis of the different root zones revealed similar arrangements of cells in those zones compared with the wild type, but reduced cell length was observed in the spr1 roots,<br /> suggesting that reduced root length in spr1 resulted from reduced cell elongation.<br /> <br /> ==References==<br /> Please input cited references here.<br /> Beemster GTS, Fiorani F, Inze´ D. 2003. Cell cycle: the key to plant growth control? Trends in Plant Science 8: 154–158.<br /> Rebouillat J, Dievart A, Verdeil J, Escoute J, Giese G, Breitler J, Gantet P, Espeout S, Guiderdoni E, Pe´rin C. 2009. Molecular genetics of rice root development. Rice 2: 15–34.<br /> Liqiang Jia;Zhongchang Wu;Xi Hao;Chris Carrie;Libin Zheng;James Whelan;Yunrong Wu;Shoufeng Wang;Ping Wu;Chuanzao Mao. Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa New Phytologist, 2011, 189(3): 843-855.<br /> Jia LiQiang . Is Reactive Oxygen Species (ROS) the underlying factor for inhibited root growth in Osspr1? Plant Signaling &amp; Behavior, 2011, 6(7): 1024-1025.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0898300|<br /> Description = Armadillo-like helical domain containing protein|<br /> Version = NM_001051627.1 GI:115441624 GeneID:4325109|<br /> Length = 6243 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0898300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:40808134..40814376|<br /> CDS = 40808350..40808496,40808589..40808780,40808893..40809024,40809123..40809260,40809362..40809562&lt;br&gt;,40809661..40809788,40810023..40810098,40810453..40810602,40810698..40811057&lt;br&gt;,40811144..40811296,40811384..40811676,40811792..40811988,40812071..40812228&lt;br&gt;,40812533..40812580,40812912..40813040,40813123..40813237,40813321..40813436&lt;br&gt;,40813737..40813811,40813982..40814116|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaaaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaaatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgatag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MGVMSRRVLPACSSLCYFCPSLRARSRQPVKRYKKIIAEIYQLP PDGEPNDRRIGKLCDYVSRNPTRIPKITEYLEERCYKDLRHENFTLAKVVPCIYRKLL CSCKDHTPLLATSTLSIIRTLLDQRMNDDLRVLGCLMLVDFLNGQVDSTHMFNLEGLI PKLCQISQELREDDKGFRLRCAALQALASMVQYMGDHSHISMELDEVVSVIVSCYEVN QTLSIKEVVRLQDDDDLVINGSLTGLPVSGQNSAKVASDTMSASENPAHWARVCLRNM ASIAKEATTVWRVLDPLFRLFDSHNYWSPENGIAFSILQEMQALMDKSGQNGHLLLSF TIKHIDHKSVAKKPAKQTSILKVASLLAKHAKLKASVTIASATSDLIKHLRKCMHCAV ESPNAQNDVDKWNSALYVALEECLVQLTEKVGDVGPVLDMVGVMLENLSCTATIARTT ISSVFRTVQIAASIHKSLYNQKAFPEALFHQLLLAMMHPDKKTRVGSHRVLSTIIAPS LLCPWSGISFPIPVKGNDSQSITLLALSAFSSEAVMDEVRIKSRTHEQLQNNVKPETV VGSENGYTHTEPNSRKSPGLGIPLKDENLKFMKLNSSQLVLLLSSIWSQAPLEDNSPA NFEAMCHTYNIALLCSMTKSSSHAALVRCFQLAFSLRRMSLNQENGLQPSRRRCLYTM ASAMLIFSAKVADIPQTIPLVKAAVPEKMVDPHLCLIDDCRLVISSPQSSNSGIVYGS EEDESDARNFLSCVNKNDTQLKEIVISHFKEKFENLSEKFNGIEEQLLQEFSLDDSFP LSAPLFMETPHSCSMYAEKDDHCFDEEVIPCEMDDDDDIVFEHSGSQSDRKTSGSMAS SDVLNVNQLIESVHETARQVANAPVSANLVPYDQMKSQCEALVMEKQQKMSVLLSFKH SRTDSRGSTAENGLETNESSARSEPETQSTRKERMRRSDSASSESDRSFRLPPASPYD KFMRAAGR&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5881..6027#5597..5788#5353..5484#5117..5254#4815..5015#4589..4716#4279..4354#3775..3924#3320..3679#3081..3233#2701..2993#2389..2585#2149..2306#1797..1844#1337..1465#1140..1254#941..1056#566..640#261..395#gaggtttgatcttggtttcgatccgaagggtctcggttggctgcgtgggaagggggaggaggaagaaggcgacctgctatctaatcctctaccccctctgctgcgaaaattctctgctgtttgctcaagcatcttgctgcttggagccttggatcgaagagagttttagttcttggagttctgagaattgtgttgcttgagccgaattcgatcccaaaaaggctcttttttgggaggctttgaggaggaggaggaagaagatgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggtatggcgattactgaatcctgtcttggcatttcagccatttctgatgcttagttgtagtctctgcgatttgggtctgtggtcaaatagttctagtttctgcgaattggagtctgtggtcaaatgtgtggagggagtttgttgattgagggtgtgatgttgttgctgtaggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaaggtaacacattttgatctcctctctgtttttactagaattgtcgcgcaatctgttagcttgaatagtttagactgaatttccagtatttctgaaagcagtctttataggtaccgagtagttaccacataatggctgatttacactgcattgctttagtgtgatggtgattccttttgccagagtacaaccgatcaactttcgctagcaccgatcactagctggaatgattttaggctgtttaagtaggacttaccgagatgaatgaatttgtggaactcggaactgattttatgtctgcagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacgtaagtgtaaaggctctattctgccagtcaagcacccatagcaaatgtttgctgctgtactgaccatattattcttgattcagaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttattgacgactgtaacaaattgcacctacttggtttgctagaaatctgccatctgtttggtttaatttgagttcatgtaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtaagaaaagtttttacttgattctctgatgaaatggataaggtagtcttatatgttgcgagctagccatttagggctaatttacagtagatttgttggaccagaaataataatgtagaacaaaataaggaactacattcaactattatacaatttgttttgcaaatataacatttttttaatgttggttatattgatattgcttgagaattcccaacagataatcctatacttgcattactggttcttgccaactgatgccattaaatcagtctattgattaaatttaactttttaatcctaatatactcatttaccactttgtaatcaggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagtaagtccttatcactctgtgttgggctagaatgttaagagttaagacttaattctcattcatggttgatagatgcctcctggtgaatatttgttctcttgttggaattgcataagattcatctttggagcatagacaagttgatgccattaatactacagatagacttgttgaataagtggaagatatttgattagtctatgaatagaaaaaaaaagtagcatgatagtttcatttgaagtgcgccagaaacagtaattcagtaagggttctgattagtctctgaattcattcttgaacccaggttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtgagaatttctgctaatgtaacggcccatttccacactcgatttgtgcccttgtcatcttaatgacttagtgtgttgacaggtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcaggtgcaaaatttttcacccatgctatttgtttctctttttgtgccattttttttggttttgttagtgtttaacagcagaaagatttagtgctcttctaaatcttgtttcttaacagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttcattatgctgtattattcctttgttacattactacattttgctttcatcagagagacattaaaggctgttaattcattgaataggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcaagttttctagtgtgttcaccattgcaggagaattttgctattgaacaaaagtgttaattggcaattccttgttaatatttcaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaagtatgtactccaactagtattcttattgaacttggtattttctggtcttggtgttttcggatgtccagctgacacattttcttccactgaagcagaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaaggtaggtcacttaatctagttcattcttgttatacaataggcaaaaagaattgttcaagtaacttgacttaacattgttctgcatgccttgaaattatcaaagaatagccaaagggaaagataccgtcttttcccttttggcatggagttatttcatattatataatatttttttatactctatttttacacaagatagattctttagaatattttattgatgtaataggcatggattaacactttttttaagatttccctgctagtgtgagtttgactagtgatgtcaacttctcctttggacttgaaattgtgtgctatatttgtgagattgaccattgaattttcgttgcagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaagtaagtttatcctcatttgcctgctctttaaagtttttgcttttgtatatcaaaatgatgatgcaaaaaaaaagtgacaactagctagggattttttccaaataggaatatcattttttctaataccagtatattttagccattgcgtccaacttcaagtttattatatttcctatcaatctagtcatttgatacctgcttgatttattcttacattttctactgtaaataaagatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggttagttgattcctgattttgaatatgcttgctctatatatatctctaatgtgacatttttcaatttactctgaattcgcttattgatgtttttgtaggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagaggtatacattcagatttacattatgagagcgtgttttcttgatttctctgttcacaatcaagaagttatttgacgattaagtgtcttcactgcaaattgcagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggtccgtactgattctctatagttaggtgattttttgttatttatgaattgctgttgtattaacttttttggttgcatctgcgtcccttgtgcttccaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggtatgcgccatttctgtctacaacaatggcacatgaagatcactcgctattttctagatctcttacagtcccctcatccgtttgtaatcaaacgacttttttttttcttcaggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgaggcatgttcatcttgacaggaaaaaaaaaatctatccatcactactgaaatctttgaaaatgtcctgaaaaactcatcaacatcttgttgcagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgataggctggtgcaatgcaatgcgatggcgccaaggatctgatactagttttcaagcctgtatacagaaatgtttttacagatacaagtcttctaaagctgtttcttttcattgtcgccttgtattttttattcttctgagtgtaaatttctcgagaaggtgtacaaatttctgttactgttgtagagacccagaataaataaaagtataggcattggtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051627.1 RefSeq:Os01g0898300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0898300&diff=176039 2014-06-02T06:14:41Z

<p>Wuxt: /* Labs working on this gene */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study has identified a novel mitochondrial protein, OsSPR1, that is involved in maintaining Fe homeostasis in rice. The large reduction of Fe content observed in the leaves but not the roots of Osspr1 mutant plants under Fe(II) and Fe(III) nutrient supply, and the normal Fe deficiency response for both strategy I and II marker genes in the leaves but not the roots indicate a defect in Fe homeostasis. In addition, root development was also compromised in Osspr1 mutant plants. It is likely that the defective roots of Osspr1 plants will have a secondary effect on nutrient uptake. However, if reduced Fe uptake as a result of damaged roots was the primary reason for the reduced Fe content in the leaves, the Fe content and the Fe deficiency response would have been duced and activated, respectively, in the roots. However, the Fe content in roots of Osspr1 mutant plants was similar to that of wild-type plants (Fig. 6e). Also, no Fe deficiency response was observed in roots (Fig. 7). However, a lower Fe content was observed in the mutant leaves, which was inconsistent with the induction of Fe-responsive genes (Figs 6c, 7). These results indicate that OsSPR1 plays a role in maintaining Fe (and other metal) homeostasis between roots and leaves.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The expression pattern of OsSPR1 was determined usingt he GUS gene driven by the OsSPR1 promoter transferred into wild-type plants. GUS staining in transgenic plants indicated that OsSPR1 was ubiquitously expressed in all plant organs, including the root, leaf, stem and spikelet (Fig. 3a–r). OsSPR1 was expressed in primary and lateral roots, both in mature regions and in root tips. Strong expression was observed in the root tip (in the meristem and lateral root initiation regions), where dividing cells are present. The expression of OsSPR1 in the root is mainly associated with vascular tissues (Fig. 3b,d,h). Semiquantitative RT-PCR analysis using SPR1-specific primers showed that SPR1 was expressed at relatively similar levelsin all tissues, including the root, stem base, stem, leaf and panicles (Fig. S3), consistent with the GUS staining patterns observed.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> Root elongation is determined by two successive processes: cell elongation and cell proliferation (Beemster et al., 2003). In rice, several genes controlling division and differentiation in root growth and development have been identified through genetic screens (Rebouillat et al., 2009). Here, we cloned OsSPR1, a novel mitochondrial gene involved in root cell elongation. Loss of function of OsSPR1 resulted in defective root elongation, including primary root, adventitious root and lateral root elongation; notably, the defects were more severe in lateral roots and adventitious roots. Analysis of the different root zones revealed similar arrangements of cells in those zones compared with the wild type, but reduced cell length was observed in the spr1 roots,<br /> suggesting that reduced root length in spr1 resulted from reduced cell elongation.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0898300|<br /> Description = Armadillo-like helical domain containing protein|<br /> Version = NM_001051627.1 GI:115441624 GeneID:4325109|<br /> Length = 6243 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0898300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:40808134..40814376|<br /> CDS = 40808350..40808496,40808589..40808780,40808893..40809024,40809123..40809260,40809362..40809562&lt;br&gt;,40809661..40809788,40810023..40810098,40810453..40810602,40810698..40811057&lt;br&gt;,40811144..40811296,40811384..40811676,40811792..40811988,40812071..40812228&lt;br&gt;,40812533..40812580,40812912..40813040,40813123..40813237,40813321..40813436&lt;br&gt;,40813737..40813811,40813982..40814116|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaaaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaaatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgatag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MGVMSRRVLPACSSLCYFCPSLRARSRQPVKRYKKIIAEIYQLP PDGEPNDRRIGKLCDYVSRNPTRIPKITEYLEERCYKDLRHENFTLAKVVPCIYRKLL CSCKDHTPLLATSTLSIIRTLLDQRMNDDLRVLGCLMLVDFLNGQVDSTHMFNLEGLI PKLCQISQELREDDKGFRLRCAALQALASMVQYMGDHSHISMELDEVVSVIVSCYEVN QTLSIKEVVRLQDDDDLVINGSLTGLPVSGQNSAKVASDTMSASENPAHWARVCLRNM ASIAKEATTVWRVLDPLFRLFDSHNYWSPENGIAFSILQEMQALMDKSGQNGHLLLSF TIKHIDHKSVAKKPAKQTSILKVASLLAKHAKLKASVTIASATSDLIKHLRKCMHCAV ESPNAQNDVDKWNSALYVALEECLVQLTEKVGDVGPVLDMVGVMLENLSCTATIARTT ISSVFRTVQIAASIHKSLYNQKAFPEALFHQLLLAMMHPDKKTRVGSHRVLSTIIAPS LLCPWSGISFPIPVKGNDSQSITLLALSAFSSEAVMDEVRIKSRTHEQLQNNVKPETV VGSENGYTHTEPNSRKSPGLGIPLKDENLKFMKLNSSQLVLLLSSIWSQAPLEDNSPA NFEAMCHTYNIALLCSMTKSSSHAALVRCFQLAFSLRRMSLNQENGLQPSRRRCLYTM ASAMLIFSAKVADIPQTIPLVKAAVPEKMVDPHLCLIDDCRLVISSPQSSNSGIVYGS EEDESDARNFLSCVNKNDTQLKEIVISHFKEKFENLSEKFNGIEEQLLQEFSLDDSFP LSAPLFMETPHSCSMYAEKDDHCFDEEVIPCEMDDDDDIVFEHSGSQSDRKTSGSMAS SDVLNVNQLIESVHETARQVANAPVSANLVPYDQMKSQCEALVMEKQQKMSVLLSFKH SRTDSRGSTAENGLETNESSARSEPETQSTRKERMRRSDSASSESDRSFRLPPASPYD KFMRAAGR&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5881..6027#5597..5788#5353..5484#5117..5254#4815..5015#4589..4716#4279..4354#3775..3924#3320..3679#3081..3233#2701..2993#2389..2585#2149..2306#1797..1844#1337..1465#1140..1254#941..1056#566..640#261..395#gaggtttgatcttggtttcgatccgaagggtctcggttggctgcgtgggaagggggaggaggaagaaggcgacctgctatctaatcctctaccccctctgctgcgaaaattctctgctgtttgctcaagcatcttgctgcttggagccttggatcgaagagagttttagttcttggagttctgagaattgtgttgcttgagccgaattcgatcccaaaaaggctcttttttgggaggctttgaggaggaggaggaagaagatgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggtatggcgattactgaatcctgtcttggcatttcagccatttctgatgcttagttgtagtctctgcgatttgggtctgtggtcaaatagttctagtttctgcgaattggagtctgtggtcaaatgtgtggagggagtttgttgattgagggtgtgatgttgttgctgtaggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaaggtaacacattttgatctcctctctgtttttactagaattgtcgcgcaatctgttagcttgaatagtttagactgaatttccagtatttctgaaagcagtctttataggtaccgagtagttaccacataatggctgatttacactgcattgctttagtgtgatggtgattccttttgccagagtacaaccgatcaactttcgctagcaccgatcactagctggaatgattttaggctgtttaagtaggacttaccgagatgaatgaatttgtggaactcggaactgattttatgtctgcagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacgtaagtgtaaaggctctattctgccagtcaagcacccatagcaaatgtttgctgctgtactgaccatattattcttgattcagaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttattgacgactgtaacaaattgcacctacttggtttgctagaaatctgccatctgtttggtttaatttgagttcatgtaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtaagaaaagtttttacttgattctctgatgaaatggataaggtagtcttatatgttgcgagctagccatttagggctaatttacagtagatttgttggaccagaaataataatgtagaacaaaataaggaactacattcaactattatacaatttgttttgcaaatataacatttttttaatgttggttatattgatattgcttgagaattcccaacagataatcctatacttgcattactggttcttgccaactgatgccattaaatcagtctattgattaaatttaactttttaatcctaatatactcatttaccactttgtaatcaggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagtaagtccttatcactctgtgttgggctagaatgttaagagttaagacttaattctcattcatggttgatagatgcctcctggtgaatatttgttctcttgttggaattgcataagattcatctttggagcatagacaagttgatgccattaatactacagatagacttgttgaataagtggaagatatttgattagtctatgaatagaaaaaaaaagtagcatgatagtttcatttgaagtgcgccagaaacagtaattcagtaagggttctgattagtctctgaattcattcttgaacccaggttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtgagaatttctgctaatgtaacggcccatttccacactcgatttgtgcccttgtcatcttaatgacttagtgtgttgacaggtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcaggtgcaaaatttttcacccatgctatttgtttctctttttgtgccattttttttggttttgttagtgtttaacagcagaaagatttagtgctcttctaaatcttgtttcttaacagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttcattatgctgtattattcctttgttacattactacattttgctttcatcagagagacattaaaggctgttaattcattgaataggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcaagttttctagtgtgttcaccattgcaggagaattttgctattgaacaaaagtgttaattggcaattccttgttaatatttcaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaagtatgtactccaactagtattcttattgaacttggtattttctggtcttggtgttttcggatgtccagctgacacattttcttccactgaagcagaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaaggtaggtcacttaatctagttcattcttgttatacaataggcaaaaagaattgttcaagtaacttgacttaacattgttctgcatgccttgaaattatcaaagaatagccaaagggaaagataccgtcttttcccttttggcatggagttatttcatattatataatatttttttatactctatttttacacaagatagattctttagaatattttattgatgtaataggcatggattaacactttttttaagatttccctgctagtgtgagtttgactagtgatgtcaacttctcctttggacttgaaattgtgtgctatatttgtgagattgaccattgaattttcgttgcagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaagtaagtttatcctcatttgcctgctctttaaagtttttgcttttgtatatcaaaatgatgatgcaaaaaaaaagtgacaactagctagggattttttccaaataggaatatcattttttctaataccagtatattttagccattgcgtccaacttcaagtttattatatttcctatcaatctagtcatttgatacctgcttgatttattcttacattttctactgtaaataaagatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggttagttgattcctgattttgaatatgcttgctctatatatatctctaatgtgacatttttcaatttactctgaattcgcttattgatgtttttgtaggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagaggtatacattcagatttacattatgagagcgtgttttcttgatttctctgttcacaatcaagaagttatttgacgattaagtgtcttcactgcaaattgcagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggtccgtactgattctctatagttaggtgattttttgttatttatgaattgctgttgtattaacttttttggttgcatctgcgtcccttgtgcttccaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggtatgcgccatttctgtctacaacaatggcacatgaagatcactcgctattttctagatctcttacagtcccctcatccgtttgtaatcaaacgacttttttttttcttcaggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgaggcatgttcatcttgacaggaaaaaaaaaatctatccatcactactgaaatctttgaaaatgtcctgaaaaactcatcaacatcttgttgcagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgataggctggtgcaatgcaatgcgatggcgccaaggatctgatactagttttcaagcctgtatacagaaatgtttttacagatacaagtcttctaaagctgtttcttttcattgtcgccttgtattttttattcttctgagtgtaaatttctcgagaaggtgtacaaatttctgttactgttgtagagacccagaataaataaaagtataggcattggtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051627.1 RefSeq:Os01g0898300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0898300&diff=176038 2014-06-02T06:11:23Z

<p>Wuxt: /* Function */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> This study has identified a novel mitochondrial protein, OsSPR1, that is involved in maintaining Fe homeostasis in rice. The large reduction of Fe content observed in the leaves but not the roots of Osspr1 mutant plants under Fe(II) and Fe(III) nutrient supply, and the normal Fe deficiency response for both strategy I and II marker genes in the leaves but not the roots indicate a defect in Fe homeostasis. In addition, root development was also compromised in Osspr1 mutant plants. It is likely that the defective roots of Osspr1 plants will have a secondary effect on nutrient uptake. However, if reduced Fe uptake as a result of damaged roots was the primary reason for the reduced Fe content in the leaves, the Fe content and the Fe deficiency response would have been duced and activated, respectively, in the roots. However, the Fe content in roots of Osspr1 mutant plants was similar to that of wild-type plants (Fig. 6e). Also, no Fe deficiency response was observed in roots (Fig. 7). However, a lower Fe content was observed in the mutant leaves, which was inconsistent with the induction of Fe-responsive genes (Figs 6c, 7). These results indicate that OsSPR1 plays a role in maintaining Fe (and other metal) homeostasis between roots and leaves.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The expression pattern of OsSPR1 was determined usingt he GUS gene driven by the OsSPR1 promoter transferred into wild-type plants. GUS staining in transgenic plants indicated that OsSPR1 was ubiquitously expressed in all plant organs, including the root, leaf, stem and spikelet (Fig. 3a–r). OsSPR1 was expressed in primary and lateral roots, both in mature regions and in root tips. Strong expression was observed in the root tip (in the meristem and lateral root initiation regions), where dividing cells are present. The expression of OsSPR1 in the root is mainly associated with vascular tissues (Fig. 3b,d,h). Semiquantitative RT-PCR analysis using SPR1-specific primers showed that SPR1 was expressed at relatively similar levelsin all tissues, including the root, stem base, stem, leaf and panicles (Fig. S3), consistent with the GUS staining patterns observed.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0898300|<br /> Description = Armadillo-like helical domain containing protein|<br /> Version = NM_001051627.1 GI:115441624 GeneID:4325109|<br /> Length = 6243 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0898300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:40808134..40814376|<br /> CDS = 40808350..40808496,40808589..40808780,40808893..40809024,40809123..40809260,40809362..40809562&lt;br&gt;,40809661..40809788,40810023..40810098,40810453..40810602,40810698..40811057&lt;br&gt;,40811144..40811296,40811384..40811676,40811792..40811988,40812071..40812228&lt;br&gt;,40812533..40812580,40812912..40813040,40813123..40813237,40813321..40813436&lt;br&gt;,40813737..40813811,40813982..40814116|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaaaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaaatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgatag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MGVMSRRVLPACSSLCYFCPSLRARSRQPVKRYKKIIAEIYQLP PDGEPNDRRIGKLCDYVSRNPTRIPKITEYLEERCYKDLRHENFTLAKVVPCIYRKLL CSCKDHTPLLATSTLSIIRTLLDQRMNDDLRVLGCLMLVDFLNGQVDSTHMFNLEGLI PKLCQISQELREDDKGFRLRCAALQALASMVQYMGDHSHISMELDEVVSVIVSCYEVN QTLSIKEVVRLQDDDDLVINGSLTGLPVSGQNSAKVASDTMSASENPAHWARVCLRNM ASIAKEATTVWRVLDPLFRLFDSHNYWSPENGIAFSILQEMQALMDKSGQNGHLLLSF TIKHIDHKSVAKKPAKQTSILKVASLLAKHAKLKASVTIASATSDLIKHLRKCMHCAV ESPNAQNDVDKWNSALYVALEECLVQLTEKVGDVGPVLDMVGVMLENLSCTATIARTT ISSVFRTVQIAASIHKSLYNQKAFPEALFHQLLLAMMHPDKKTRVGSHRVLSTIIAPS LLCPWSGISFPIPVKGNDSQSITLLALSAFSSEAVMDEVRIKSRTHEQLQNNVKPETV VGSENGYTHTEPNSRKSPGLGIPLKDENLKFMKLNSSQLVLLLSSIWSQAPLEDNSPA NFEAMCHTYNIALLCSMTKSSSHAALVRCFQLAFSLRRMSLNQENGLQPSRRRCLYTM ASAMLIFSAKVADIPQTIPLVKAAVPEKMVDPHLCLIDDCRLVISSPQSSNSGIVYGS EEDESDARNFLSCVNKNDTQLKEIVISHFKEKFENLSEKFNGIEEQLLQEFSLDDSFP LSAPLFMETPHSCSMYAEKDDHCFDEEVIPCEMDDDDDIVFEHSGSQSDRKTSGSMAS SDVLNVNQLIESVHETARQVANAPVSANLVPYDQMKSQCEALVMEKQQKMSVLLSFKH SRTDSRGSTAENGLETNESSARSEPETQSTRKERMRRSDSASSESDRSFRLPPASPYD KFMRAAGR&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5881..6027#5597..5788#5353..5484#5117..5254#4815..5015#4589..4716#4279..4354#3775..3924#3320..3679#3081..3233#2701..2993#2389..2585#2149..2306#1797..1844#1337..1465#1140..1254#941..1056#566..640#261..395#gaggtttgatcttggtttcgatccgaagggtctcggttggctgcgtgggaagggggaggaggaagaaggcgacctgctatctaatcctctaccccctctgctgcgaaaattctctgctgtttgctcaagcatcttgctgcttggagccttggatcgaagagagttttagttcttggagttctgagaattgtgttgcttgagccgaattcgatcccaaaaaggctcttttttgggaggctttgaggaggaggaggaagaagatgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggtatggcgattactgaatcctgtcttggcatttcagccatttctgatgcttagttgtagtctctgcgatttgggtctgtggtcaaatagttctagtttctgcgaattggagtctgtggtcaaatgtgtggagggagtttgttgattgagggtgtgatgttgttgctgtaggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaaggtaacacattttgatctcctctctgtttttactagaattgtcgcgcaatctgttagcttgaatagtttagactgaatttccagtatttctgaaagcagtctttataggtaccgagtagttaccacataatggctgatttacactgcattgctttagtgtgatggtgattccttttgccagagtacaaccgatcaactttcgctagcaccgatcactagctggaatgattttaggctgtttaagtaggacttaccgagatgaatgaatttgtggaactcggaactgattttatgtctgcagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacgtaagtgtaaaggctctattctgccagtcaagcacccatagcaaatgtttgctgctgtactgaccatattattcttgattcagaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttattgacgactgtaacaaattgcacctacttggtttgctagaaatctgccatctgtttggtttaatttgagttcatgtaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtaagaaaagtttttacttgattctctgatgaaatggataaggtagtcttatatgttgcgagctagccatttagggctaatttacagtagatttgttggaccagaaataataatgtagaacaaaataaggaactacattcaactattatacaatttgttttgcaaatataacatttttttaatgttggttatattgatattgcttgagaattcccaacagataatcctatacttgcattactggttcttgccaactgatgccattaaatcagtctattgattaaatttaactttttaatcctaatatactcatttaccactttgtaatcaggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagtaagtccttatcactctgtgttgggctagaatgttaagagttaagacttaattctcattcatggttgatagatgcctcctggtgaatatttgttctcttgttggaattgcataagattcatctttggagcatagacaagttgatgccattaatactacagatagacttgttgaataagtggaagatatttgattagtctatgaatagaaaaaaaaagtagcatgatagtttcatttgaagtgcgccagaaacagtaattcagtaagggttctgattagtctctgaattcattcttgaacccaggttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtgagaatttctgctaatgtaacggcccatttccacactcgatttgtgcccttgtcatcttaatgacttagtgtgttgacaggtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcaggtgcaaaatttttcacccatgctatttgtttctctttttgtgccattttttttggttttgttagtgtttaacagcagaaagatttagtgctcttctaaatcttgtttcttaacagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttcattatgctgtattattcctttgttacattactacattttgctttcatcagagagacattaaaggctgttaattcattgaataggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcaagttttctagtgtgttcaccattgcaggagaattttgctattgaacaaaagtgttaattggcaattccttgttaatatttcaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaagtatgtactccaactagtattcttattgaacttggtattttctggtcttggtgttttcggatgtccagctgacacattttcttccactgaagcagaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaaggtaggtcacttaatctagttcattcttgttatacaataggcaaaaagaattgttcaagtaacttgacttaacattgttctgcatgccttgaaattatcaaagaatagccaaagggaaagataccgtcttttcccttttggcatggagttatttcatattatataatatttttttatactctatttttacacaagatagattctttagaatattttattgatgtaataggcatggattaacactttttttaagatttccctgctagtgtgagtttgactagtgatgtcaacttctcctttggacttgaaattgtgtgctatatttgtgagattgaccattgaattttcgttgcagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaagtaagtttatcctcatttgcctgctctttaaagtttttgcttttgtatatcaaaatgatgatgcaaaaaaaaagtgacaactagctagggattttttccaaataggaatatcattttttctaataccagtatattttagccattgcgtccaacttcaagtttattatatttcctatcaatctagtcatttgatacctgcttgatttattcttacattttctactgtaaataaagatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggttagttgattcctgattttgaatatgcttgctctatatatatctctaatgtgacatttttcaatttactctgaattcgcttattgatgtttttgtaggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagaggtatacattcagatttacattatgagagcgtgttttcttgatttctctgttcacaatcaagaagttatttgacgattaagtgtcttcactgcaaattgcagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggtccgtactgattctctatagttaggtgattttttgttatttatgaattgctgttgtattaacttttttggttgcatctgcgtcccttgtgcttccaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggtatgcgccatttctgtctacaacaatggcacatgaagatcactcgctattttctagatctcttacagtcccctcatccgtttgtaatcaaacgacttttttttttcttcaggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgaggcatgttcatcttgacaggaaaaaaaaaatctatccatcactactgaaatctttgaaaatgtcctgaaaaactcatcaacatcttgttgcagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgataggctggtgcaatgcaatgcgatggcgccaaggatctgatactagttttcaagcctgtatacagaaatgtttttacagatacaagtcttctaaagctgtttcttttcattgtcgccttgtattttttattcttctgagtgtaaatttctcgagaaggtgtacaaatttctgttactgttgtagagacccagaataaataaaagtataggcattggtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051627.1 RefSeq:Os01g0898300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Wuxt https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0898300&diff=176037 2014-06-02T06:05:26Z

<p>Wuxt: /* Expression */</p> <hr /> <div>Please input one-sentence summary here.<br /> <br /> ==Annotated Information==<br /> ===Function===<br /> Please input function information here.<br /> <br /> ===Expression===<br /> Please input expression information here.<br /> The expression pattern of OsSPR1 was determined usingt he GUS gene driven by the OsSPR1 promoter transferred into wild-type plants. GUS staining in transgenic plants indicated that OsSPR1 was ubiquitously expressed in all plant organs, including the root, leaf, stem and spikelet (Fig. 3a–r). OsSPR1 was expressed in primary and lateral roots, both in mature regions and in root tips. Strong expression was observed in the root tip (in the meristem and lateral root initiation regions), where dividing cells are present. The expression of OsSPR1 in the root is mainly associated with vascular tissues (Fig. 3b,d,h). Semiquantitative RT-PCR analysis using SPR1-specific primers showed that SPR1 was expressed at relatively similar levelsin all tissues, including the root, stem base, stem, leaf and panicles (Fig. S3), consistent with the GUS staining patterns observed.<br /> <br /> ===Evolution===<br /> Please input evolution information here.<br /> <br /> You can also add sub-section(s) at will.<br /> <br /> ==Labs working on this gene==<br /> Please input related labs here.<br /> <br /> ==References==<br /> Please input cited references here.<br /> <br /> ==Structured Information==<br /> {{JaponicaGene|<br /> GeneName = Os01g0898300|<br /> Description = Armadillo-like helical domain containing protein|<br /> Version = NM_001051627.1 GI:115441624 GeneID:4325109|<br /> Length = 6243 bp|<br /> Definition = Oryza sativa Japonica Group Os01g0898300, complete gene.|<br /> Source = Oryza sativa Japonica Group<br /> <br /> ORGANISM Oryza sativa Japonica Group<br /> Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;<br /> Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP<br /> clade; Ehrhartoideae; Oryzeae; Oryza.<br /> |<br /> Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|<br /> AP = Chromosome 1:40808134..40814376|<br /> CDS = 40808350..40808496,40808589..40808780,40808893..40809024,40809123..40809260,40809362..40809562&lt;br&gt;,40809661..40809788,40810023..40810098,40810453..40810602,40810698..40811057&lt;br&gt;,40811144..40811296,40811384..40811676,40811792..40811988,40812071..40812228&lt;br&gt;,40812533..40812580,40812912..40813040,40813123..40813237,40813321..40813436&lt;br&gt;,40813737..40813811,40813982..40814116|<br /> GCID = &lt;gbrowseImage1&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage1&gt;|<br /> GSID = &lt;gbrowseImage2&gt;<br /> name=NC_008394:40808134..40814376<br /> source=RiceChromosome01<br /> preset=GeneLocation<br /> &lt;/gbrowseImage2&gt;|<br /> CDNA = &lt;cdnaseq&gt;atgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaaaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaaatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgatag&lt;/cdnaseq&gt;|<br /> AA = &lt;aaseq&gt;MGVMSRRVLPACSSLCYFCPSLRARSRQPVKRYKKIIAEIYQLP PDGEPNDRRIGKLCDYVSRNPTRIPKITEYLEERCYKDLRHENFTLAKVVPCIYRKLL CSCKDHTPLLATSTLSIIRTLLDQRMNDDLRVLGCLMLVDFLNGQVDSTHMFNLEGLI PKLCQISQELREDDKGFRLRCAALQALASMVQYMGDHSHISMELDEVVSVIVSCYEVN QTLSIKEVVRLQDDDDLVINGSLTGLPVSGQNSAKVASDTMSASENPAHWARVCLRNM ASIAKEATTVWRVLDPLFRLFDSHNYWSPENGIAFSILQEMQALMDKSGQNGHLLLSF TIKHIDHKSVAKKPAKQTSILKVASLLAKHAKLKASVTIASATSDLIKHLRKCMHCAV ESPNAQNDVDKWNSALYVALEECLVQLTEKVGDVGPVLDMVGVMLENLSCTATIARTT ISSVFRTVQIAASIHKSLYNQKAFPEALFHQLLLAMMHPDKKTRVGSHRVLSTIIAPS LLCPWSGISFPIPVKGNDSQSITLLALSAFSSEAVMDEVRIKSRTHEQLQNNVKPETV VGSENGYTHTEPNSRKSPGLGIPLKDENLKFMKLNSSQLVLLLSSIWSQAPLEDNSPA NFEAMCHTYNIALLCSMTKSSSHAALVRCFQLAFSLRRMSLNQENGLQPSRRRCLYTM ASAMLIFSAKVADIPQTIPLVKAAVPEKMVDPHLCLIDDCRLVISSPQSSNSGIVYGS EEDESDARNFLSCVNKNDTQLKEIVISHFKEKFENLSEKFNGIEEQLLQEFSLDDSFP LSAPLFMETPHSCSMYAEKDDHCFDEEVIPCEMDDDDDIVFEHSGSQSDRKTSGSMAS SDVLNVNQLIESVHETARQVANAPVSANLVPYDQMKSQCEALVMEKQQKMSVLLSFKH SRTDSRGSTAENGLETNESSARSEPETQSTRKERMRRSDSASSESDRSFRLPPASPYD KFMRAAGR&lt;/aaseq&gt;|<br /> DNA = &lt;dnaseqindica&gt;5881..6027#5597..5788#5353..5484#5117..5254#4815..5015#4589..4716#4279..4354#3775..3924#3320..3679#3081..3233#2701..2993#2389..2585#2149..2306#1797..1844#1337..1465#1140..1254#941..1056#566..640#261..395#gaggtttgatcttggtttcgatccgaagggtctcggttggctgcgtgggaagggggaggaggaagaaggcgacctgctatctaatcctctaccccctctgctgcgaaaattctctgctgtttgctcaagcatcttgctgcttggagccttggatcgaagagagttttagttcttggagttctgagaattgtgttgcttgagccgaattcgatcccaaaaaggctcttttttgggaggctttgaggaggaggaggaagaagatgggggtgatgtcgcggcgggtgctgcctgcctgcagcagcctctgctacttctgcccctcgctgcgggcgcgctcgcgtcagcccgtcaagaggtacaagaagatcatcgcggagatctaccagcttccaccggtatggcgattactgaatcctgtcttggcatttcagccatttctgatgcttagttgtagtctctgcgatttgggtctgtggtcaaatagttctagtttctgcgaattggagtctgtggtcaaatgtgtggagggagtttgttgattgagggtgtgatgttgttgctgtaggatggtgaaccgaatgataggaggattgggaagctctgtgattatgtctcgagaaatccgacgcggatacccaaggtaacacattttgatctcctctctgtttttactagaattgtcgcgcaatctgttagcttgaatagtttagactgaatttccagtatttctgaaagcagtctttataggtaccgagtagttaccacataatggctgatttacactgcattgctttagtgtgatggtgattccttttgccagagtacaaccgatcaactttcgctagcaccgatcactagctggaatgattttaggctgtttaagtaggacttaccgagatgaatgaatttgtggaactcggaactgattttatgtctgcagatcacagagtaccttgaggagaggtgctataaagatctgagacatgagaatttcaccttggccaaagttgtaccatgtatatacaggaagcttctgtgttcatgcaaggatcatacgtaagtgtaaaggctctattctgccagtcaagcacccatagcaaatgtttgctgctgtactgaccatattattcttgattcagaccattgctggccacaagcacattgagcattattcgtactcttctcgatcagagaatgaatgatgatttgcgggtcctgggttgtctaatgcttgttgactttctcaacggccaggttattgacgactgtaacaaattgcacctacttggtttgctagaaatctgccatctgtttggtttaatttgagttcatgtaggttgatagcacgcatatgtttaatctggaaggcctcataccaaaactttgccaaattagtcaagaactaagggaagatgacaaagggttccgcctccgatgtgctgcacttcaggctcttgcttctatggtaagaaaagtttttacttgattctctgatgaaatggataaggtagtcttatatgttgcgagctagccatttagggctaatttacagtagatttgttggaccagaaataataatgtagaacaaaataaggaactacattcaactattatacaatttgttttgcaaatataacatttttttaatgttggttatattgatattgcttgagaattcccaacagataatcctatacttgcattactggttcttgccaactgatgccattaaatcagtctattgattaaatttaactttttaatcctaatatactcatttaccactttgtaatcaggtccaatacatgggtgaccactcacacatttccatggaacttgatgaagtaagtccttatcactctgtgttgggctagaatgttaagagttaagacttaattctcattcatggttgatagatgcctcctggtgaatatttgttctcttgttggaattgcataagattcatctttggagcatagacaagttgatgccattaatactacagatagacttgttgaataagtggaagatatttgattagtctatgaatagaaaaaaaaagtagcatgatagtttcatttgaagtgcgccagaaacagtaattcagtaagggttctgattagtctctgaattcattcttgaacccaggttgtgtccgtgatcgtaagttgttatgaggttaatcaaactctctcaataaaagaggttgtcaggcttcaagatgatgatgatttggtaatcaatggaagtctgactgggttgccagtatctgggcaaaatagtgccaaagtggcatctgatacaatgtgagaatttctgctaatgtaacggcccatttccacactcgatttgtgcccttgtcatcttaatgacttagtgtgttgacaggtctgcatctgaaaatccagcacattgggcaagggtttgcttacgtaatatggccagtatcgccaaggaggcaacaacagtgtggcgtgttcttgatcctcttttccgcctttttgatagccacaactattggtctcctgaaaatgggattgcattttctattctacaagaaatgcaggcactgatggacaaatcaggtgcaaaatttttcacccatgctatttgtttctctttttgtgccattttttttggttttgttagtgtttaacagcagaaagatttagtgctcttctaaatcttgtttcttaacagggcaaaatggccatttgctgttatctttcaccataaaacacatagatcataagagtgttgccaagaagcctgccaagcaaacaagcattttaaaagttgcttcgcttcttgcaaaacatgcaaagttgaaggcgtcggtgacaatagcaagtgcaacaagcgacttaataaagcacttgcgcaaatgcatgcattgtgctgttgaatcaccaaatgctcaaaatgatgttgacaagtggaacagtgcactttatgtggccttggaggagtgcctggtgcaattgacagaaaaggttcattatgctgtattattcctttgttacattactacattttgctttcatcagagagacattaaaggctgttaattcattgaataggttggtgatgttgggcctgttcttgacatggtgggagtaatgcttgagaatctctcttgtactgccaccattgccagaacaacaatttcatcagttttccgcacagtgcaaatagcagcttccatccacaagtcattgtacaaccagaaggcaagttttctagtgtgttcaccattgcaggagaattttgctattgaacaaaagtgttaattggcaattccttgttaatatttcaggcatttcctgaagctttgtttcatcagcttctcttagcaatgatgcacccagacaaaaagacaagggttggttcacatcgtgtcctatccaccattattgcaccttcacttctatgcccatggtcaggcataagttttcctatccctgtgaagggcaatgattcacagagcattactttgttggctctttctgccttttcttctgaggccgtaatggatgaagtccggatcaaaagcaggacccatgaacagttacaaaacaatgtaaaaccagaaactgtagttggctctgagaacggatacacacatacagaaccaaattcaaggaagagccctggtcttggaattccattgaaagatgaagtatgtactccaactagtattcttattgaacttggtattttctggtcttggtgttttcggatgtccagctgacacattttcttccactgaagcagaatcttaagttcatgaagctgaacagtagccaacttgttctcctcctttcatcaatttggagtcaagcacctctagaagataactcaccagcaaattttgaagcaatgtgccatacctacaatattgctttattgtgttcaatgacaaaggtaggtcacttaatctagttcattcttgttatacaataggcaaaaagaattgttcaagtaacttgacttaacattgttctgcatgccttgaaattatcaaagaatagccaaagggaaagataccgtcttttcccttttggcatggagttatttcatattatataatatttttttatactctatttttacacaagatagattctttagaatattttattgatgtaataggcatggattaacactttttttaagatttccctgctagtgtgagtttgactagtgatgtcaacttctcctttggacttgaaattgtgtgctatatttgtgagattgaccattgaattttcgttgcagagctctagtcacgcagcattagttcgatgtttccagttggccttctctctcaggaggatgtcccttaaccaagaaagtaagtttatcctcatttgcctgctctttaaagtttttgcttttgtatatcaaaatgatgatgcaaaaaaaaagtgacaactagctagggattttttccaaataggaatatcattttttctaataccagtatattttagccattgcgtccaacttcaagtttattatatttcctatcaatctagtcatttgatacctgcttgatttattcttacattttctactgtaaataaagatggtttgcagccatcacgtaggaggtgtttgtatacaatggcatcagcaatgctgattttttcagcaaaagttgctgatattcctcagacaattcctcttgtgaaagcagcagttccagagaaaatggttagttgattcctgattttgaatatgcttgctctatatatatctctaatgtgacatttttcaatttactctgaattcgcttattgatgtttttgtaggtcgaccctcatctttgcctgattgatgattgcagactcgttattagttcaccacagtcatctaacagtgggatagtttatggttctgaggaagatgaaagtgatgcacgcaattttctttcttgtgtaaataaaaatgatacacagttaaaagaaattgtgatatcccacttcaaggagaagtttgaaaatctatcagaggtatacattcagatttacattatgagagcgtgttttcttgatttctctgttcacaatcaagaagttatttgacgattaagtgtcttcactgcaaattgcagaagtttaatggtatagaagagcagcttcttcaggagttttccctggatgattcattccctctcagtgctccactattcatggaaacaccacactcttgttcgatgtacgctgaaaaggatgaccattgttttgatgaggtccgtactgattctctatagttaggtgattttttgttatttatgaattgctgttgtattaacttttttggttgcatctgcgtcccttgtgcttccaggaagtcattccttgtgagatggatgatgacgatgacatcgtttttgaacacagtggatcgcaatctgacaggaaaacctctggatctatggcttcatcagatgttctaaacgtcaatcaactaatagaatcggtatgcgccatttctgtctacaacaatggcacatgaagatcactcgctattttctagatctcttacagtcccctcatccgtttgtaatcaaacgacttttttttttcttcaggttcatgagacagcaaggcaggttgctaatgctccagtttctgccaaccttgtaccctacgatcaaatgaagagccaatgcgaagccctagtcatggagaagcaacagaagatgtctgttctcctgagcttcaaacactcgaggaccgattcccgtggctcgactgcagaaaatggactggaaacaaacgaggcatgttcatcttgacaggaaaaaaaaaatctatccatcactactgaaatctttgaaaatgtcctgaaaaactcatcaacatcttgttgcagtcatctgctcggtctgagcctgagacgcagtcgacgaggaaggagcgaatgcgacgcagcgactcggcatcaagcgaatctgaccggtcgttcaggctgccaccggcaagcccatatgacaagttcatgagagctgctgggcgataggctggtgcaatgcaatgcgatggcgccaaggatctgatactagttttcaagcctgtatacagaaatgtttttacagatacaagtcttctaaagctgtttcttttcattgtcgccttgtattttttattcttctgagtgtaaatttctcgagaaggtgtacaaatttctgttactgttgtagagacccagaataaataaaagtataggcattggtgc&lt;/dnaseqindica&gt;|<br /> Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051627.1 RefSeq:Os01g0898300]|<br /> }}<br /> [[Category:Genes]]<br /> [[Category:Japonica mRNA]]<br /> [[Category:Oryza Sativa Japonica Group]]<br /> [[Category:Japonica Genes]]<br /> [[Category:Japonica Chromosome 1]]<br /> [[Category:Chromosome 1]]</div>

Wuxt